LOCUS       DAYYGO010000001        77035 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000001 DAYYGO010000000
VERSION     DAYYGO010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 77035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.001"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(76..1221)
                     /locus_tag="ACHA5T_000001"
     CDS             complement(76..1221)
                     /locus_tag="ACHA5T_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8885614.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(1282..1929)
                     /locus_tag="ACHA5T_000002"
     CDS             complement(1282..1929)
                     /locus_tag="ACHA5T_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8885615.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            2123..2416
                     /locus_tag="ACHA5T_000003"
     CDS             2123..2416
                     /locus_tag="ACHA5T_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8885616.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            2460..2771
                     /locus_tag="ACHA5T_000004"
     CDS             2460..2771
                     /locus_tag="ACHA5T_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8885617.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            2768..3392
                     /locus_tag="ACHA5T_000005"
                     /pseudo
     CDS             2768..3392
                     /locus_tag="ACHA5T_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            3533..3691
                     /locus_tag="ACHA5T_000006"
     CDS             3533..3691
                     /locus_tag="ACHA5T_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8885618.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            3692..4054
                     /locus_tag="ACHA5T_000007"
     CDS             3692..4054
                     /locus_tag="ACHA5T_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8885619.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            4091..4939
                     /locus_tag="ACHA5T_000008"
     CDS             4091..4939
                     /locus_tag="ACHA5T_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HFM8885620.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            4929..6404
                     /locus_tag="ACHA5T_000009"
     CDS             4929..6404
                     /locus_tag="ACHA5T_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HFM8885621.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            6670..7077
                     /locus_tag="ACHA5T_000010"
     CDS             6670..7077
                     /locus_tag="ACHA5T_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HFM8885622.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            7080..7295
                     /locus_tag="ACHA5T_000011"
     CDS             7080..7295
                     /locus_tag="ACHA5T_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8885623.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            7299..7679
                     /locus_tag="ACHA5T_000012"
     CDS             7299..7679
                     /locus_tag="ACHA5T_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFM8885624.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            7813..7968
                     /locus_tag="ACHA5T_000013"
     CDS             7813..7968
                     /locus_tag="ACHA5T_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HFM8885625.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            8037..8510
                     /locus_tag="ACHA5T_000014"
     CDS             8037..8510
                     /locus_tag="ACHA5T_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFM8885626.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            8507..10201
                     /locus_tag="ACHA5T_000015"
     CDS             8507..10201
                     /locus_tag="ACHA5T_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFM8885627.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            10167..10352
                     /locus_tag="ACHA5T_000016"
     CDS             10167..10352
                     /locus_tag="ACHA5T_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8885628.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            10356..11531
                     /locus_tag="ACHA5T_000017"
     CDS             10356..11531
                     /locus_tag="ACHA5T_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8885629.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            11524..13047
                     /locus_tag="ACHA5T_000018"
     CDS             11524..13047
                     /locus_tag="ACHA5T_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM8885630.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            13103..13387
                     /locus_tag="ACHA5T_000019"
     CDS             13103..13387
                     /locus_tag="ACHA5T_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFM8885631.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            13374..13709
                     /locus_tag="ACHA5T_000020"
     CDS             13374..13709
                     /locus_tag="ACHA5T_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM8885632.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            14004..14630
                     /locus_tag="ACHA5T_000021"
     CDS             14004..14630
                     /locus_tag="ACHA5T_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8885633.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            15372..16208
                     /locus_tag="ACHA5T_000022"
     CDS             15372..16208
                     /locus_tag="ACHA5T_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFM8885634.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLINLYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            16272..16703
                     /locus_tag="ACHA5T_000023"
     CDS             16272..16703
                     /locus_tag="ACHA5T_000023"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8885635.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(16765..18087)
                     /locus_tag="ACHA5T_000024"
     CDS             complement(16765..18087)
                     /locus_tag="ACHA5T_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFM8885636.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(18241..19881)
                     /locus_tag="ACHA5T_000025"
     CDS             complement(18241..19881)
                     /locus_tag="ACHA5T_000025"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFM8885637.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            20214..20684
                     /locus_tag="ACHA5T_000026"
     CDS             20214..20684
                     /locus_tag="ACHA5T_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFM8885638.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            20906..22009
                     /locus_tag="ACHA5T_000027"
     CDS             20906..22009
                     /locus_tag="ACHA5T_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFM8885639.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLKDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            22293..22973
                     /locus_tag="ACHA5T_000028"
     CDS             22293..22973
                     /locus_tag="ACHA5T_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM8885640.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            23063..24097
                     /locus_tag="ACHA5T_000029"
     CDS             23063..24097
                     /locus_tag="ACHA5T_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFM8885641.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            24111..24446
                     /locus_tag="ACHA5T_000030"
     CDS             24111..24446
                     /locus_tag="ACHA5T_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8885642.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            24430..26910
                     /locus_tag="ACHA5T_000031"
     CDS             24430..26910
                     /locus_tag="ACHA5T_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8885643.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWKLTAGPTS"
     gene            26966..28525
                     /locus_tag="ACHA5T_000032"
     CDS             26966..28525
                     /locus_tag="ACHA5T_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8885644.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            28522..29886
                     /locus_tag="ACHA5T_000033"
     CDS             28522..29886
                     /locus_tag="ACHA5T_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFM8885645.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            30057..31127
                     /locus_tag="ACHA5T_000034"
     CDS             30057..31127
                     /locus_tag="ACHA5T_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8885646.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            31142..31810
                     /locus_tag="ACHA5T_000035"
     CDS             31142..31810
                     /locus_tag="ACHA5T_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8885647.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            31830..32408
                     /locus_tag="ACHA5T_000036"
     CDS             31830..32408
                     /locus_tag="ACHA5T_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8885648.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            32809..33720
                     /locus_tag="ACHA5T_000037"
     CDS             32809..33720
                     /locus_tag="ACHA5T_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM8885649.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(33808..34725)
                     /gene="rnhC"
                     /locus_tag="ACHA5T_000038"
     CDS             complement(33808..34725)
                     /gene="rnhC"
                     /locus_tag="ACHA5T_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFM8885650.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            34927..35364
                     /gene="zapA"
                     /locus_tag="ACHA5T_000039"
     CDS             34927..35364
                     /gene="zapA"
                     /locus_tag="ACHA5T_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFM8885651.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            35423..35971
                     /locus_tag="ACHA5T_000040"
     CDS             35423..35971
                     /locus_tag="ACHA5T_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFM8885652.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            36086..38446
                     /locus_tag="ACHA5T_000041"
     CDS             36086..38446
                     /locus_tag="ACHA5T_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFM8885653.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEEQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLYDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            38533..38847
                     /gene="trxA"
                     /locus_tag="ACHA5T_000042"
     CDS             38533..38847
                     /gene="trxA"
                     /locus_tag="ACHA5T_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFM8885654.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            39029..40807
                     /gene="uvrC"
                     /locus_tag="ACHA5T_000043"
     CDS             39029..40807
                     /gene="uvrC"
                     /locus_tag="ACHA5T_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFM8885655.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            40952..41851
                     /locus_tag="ACHA5T_000044"
     CDS             40952..41851
                     /locus_tag="ACHA5T_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8885656.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            41844..43106
                     /locus_tag="ACHA5T_000045"
     CDS             41844..43106
                     /locus_tag="ACHA5T_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8885657.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            43267..45165
                     /locus_tag="ACHA5T_000046"
     CDS             43267..45165
                     /locus_tag="ACHA5T_000046"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFM8885658.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(45495..46214)
                     /locus_tag="ACHA5T_000047"
     CDS             complement(45495..46214)
                     /locus_tag="ACHA5T_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFM8885659.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     misc_feature    46404..46451
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            46551..46883
                     /locus_tag="ACHA5T_000048"
     CDS             46551..46883
                     /locus_tag="ACHA5T_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8885660.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            46870..48873
                     /locus_tag="ACHA5T_000049"
     CDS             46870..48873
                     /locus_tag="ACHA5T_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFM8885661.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            48892..49362
                     /locus_tag="ACHA5T_000050"
     CDS             48892..49362
                     /locus_tag="ACHA5T_000050"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFM8885662.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            49383..49967
                     /locus_tag="ACHA5T_000051"
     CDS             49383..49967
                     /locus_tag="ACHA5T_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HFM8885663.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            49982..50968
                     /locus_tag="ACHA5T_000052"
     CDS             49982..50968
                     /locus_tag="ACHA5T_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFM8885664.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            50958..51269
                     /locus_tag="ACHA5T_000053"
     CDS             50958..51269
                     /locus_tag="ACHA5T_000053"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFM8885665.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            51291..53072
                     /locus_tag="ACHA5T_000054"
     CDS             51291..53072
                     /locus_tag="ACHA5T_000054"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFM8885666.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            53065..54441
                     /locus_tag="ACHA5T_000055"
     CDS             53065..54441
                     /locus_tag="ACHA5T_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFM8885667.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            54448..55083
                     /locus_tag="ACHA5T_000056"
     CDS             54448..55083
                     /locus_tag="ACHA5T_000056"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFM8885668.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            55113..56468
                     /locus_tag="ACHA5T_000057"
     CDS             55113..56468
                     /locus_tag="ACHA5T_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFM8885669.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(56660..58054)
                     /locus_tag="ACHA5T_000058"
     CDS             complement(56660..58054)
                     /locus_tag="ACHA5T_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFM8885670.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRTQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            58146..59117
                     /locus_tag="ACHA5T_000059"
     CDS             58146..59117
                     /locus_tag="ACHA5T_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFM8885671.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            59114..59896
                     /locus_tag="ACHA5T_000060"
     CDS             59114..59896
                     /locus_tag="ACHA5T_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFM8885672.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(60065..60436)
                     /locus_tag="ACHA5T_000061"
     CDS             complement(60065..60436)
                     /locus_tag="ACHA5T_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8885673.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            60570..61013
                     /locus_tag="ACHA5T_000062"
     CDS             60570..61013
                     /locus_tag="ACHA5T_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFM8885674.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(61056..61865)
                     /gene="yidA"
                     /locus_tag="ACHA5T_000063"
     CDS             complement(61056..61865)
                     /gene="yidA"
                     /locus_tag="ACHA5T_000063"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFM8885675.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(61884..63251)
                     /locus_tag="ACHA5T_000064"
     CDS             complement(61884..63251)
                     /locus_tag="ACHA5T_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8885676.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(63235..64065)
                     /locus_tag="ACHA5T_000065"
     CDS             complement(63235..64065)
                     /locus_tag="ACHA5T_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFM8885677.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            64228..64665
                     /locus_tag="ACHA5T_000066"
     CDS             64228..64665
                     /locus_tag="ACHA5T_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFM8885678.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            64741..65361
                     /gene="rpoE"
                     /locus_tag="ACHA5T_000067"
     CDS             64741..65361
                     /gene="rpoE"
                     /locus_tag="ACHA5T_000067"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFM8885679.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            65550..66056
                     /locus_tag="ACHA5T_000068"
     CDS             65550..66056
                     /locus_tag="ACHA5T_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFM8885680.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            66073..66972
                     /locus_tag="ACHA5T_000069"
     CDS             66073..66972
                     /locus_tag="ACHA5T_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFM8885681.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            67059..67943
                     /locus_tag="ACHA5T_000070"
     CDS             67059..67943
                     /locus_tag="ACHA5T_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFM8885682.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(68135..68206)
                     /locus_tag="ACHA5T_000071"
     tRNA            complement(68135..68206)
                     /locus_tag="ACHA5T_000071"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(68171..68173),aa:Arg,seq:ccg)
     gene            68266..69600
                     /gene="rpoN"
                     /locus_tag="ACHA5T_000072"
     CDS             68266..69600
                     /gene="rpoN"
                     /locus_tag="ACHA5T_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFM8885683.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            69749..70840
                     /locus_tag="ACHA5T_000073"
     CDS             69749..70840
                     /locus_tag="ACHA5T_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFM8885684.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            70881..71882
                     /gene="gap"
                     /locus_tag="ACHA5T_000074"
     CDS             70881..71882
                     /gene="gap"
                     /locus_tag="ACHA5T_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFM8885685.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            71992..73182
                     /gene="pgk"
                     /locus_tag="ACHA5T_000075"
     CDS             71992..73182
                     /gene="pgk"
                     /locus_tag="ACHA5T_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFM8885686.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            73286..74041
                     /gene="tpiA"
                     /locus_tag="ACHA5T_000076"
     CDS             73286..74041
                     /gene="tpiA"
                     /locus_tag="ACHA5T_000076"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFM8885687.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            74157..75455
                     /gene="eno"
                     /locus_tag="ACHA5T_000077"
     CDS             74157..75455
                     /gene="eno"
                     /locus_tag="ACHA5T_000077"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFM8885688.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            75854..76993
                     /locus_tag="ACHA5T_000078"
     CDS             75854..76993
                     /locus_tag="ACHA5T_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFM8885689.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT        25692 a        13253 c        16156 g        21934 t
ORIGIN      
        1 aaagctagga gaaaccttga tatgataggg tttctccttt ttttattttt tactgtatgc
       61 catattgtat gccatctagt caatatttaa gtgttgtact aatttttcaa cagtcttttc
      121 tttcgtttct tttgccaagt gaccgtaaca atcgatcgtc tgactaattt ttgtatgccg
      181 taatctagtt tgaacttcta ccatagtagc tccagcgtcc aagagcattg aagcatgagt
      241 gtgtcttgct acatgcacac cgagaaatgg aaccatagct ttttcacaaa tagtaatcaa
      301 tcttgcataa atagcgtggt gtgtgattag ttctttatag ataccacaaa aaatcatagt
      361 cggatcagtt acaccaagtg tcagcatata tttccgctga ttaatctgcc atttttttaa
      421 tatagaaatt gttctttcat ctagtggaag aaccccagca ctacaatcag ttttaggatc
      481 actgatttta tagccatttt tggattggct tagtgtttta ttgatggtga ccgtattttc
      541 ttgaaaatca atgtctgacc aattcaatgc aagggcttca ctgatcctaa ccccgctata
      601 aaagagaaag cgtagtaacg ttttatcaaa gtcgtttcga taagtttcag gtttgctgtt
      661 taggtaatca aacaaggtga tcagttgttc tttactgtaa actttgacct tttctttatg
      721 cttagtggtg gttcttttag gcattaatac gttgtctagt ggattagtct caataatccc
      781 aagattgata gcgtagctaa aaatacgctt agaagttgaa tgaagtacct tgtattggtc
      841 agtagtttca gcccagctat ttacaatttg ttgggctttt tttaccgtga cttcattcac
      901 acgaagattt ccatattttg gaagaatcca ctttctaaac ttgatttcag ttttcatgaa
      961 ggtggcttct cggacagtgg ttttatatgt ttctagccac aagtcataaa cttctttaaa
     1021 cgtttcgttg ttcgcttttt taataccgtt tttatcgtag tccacttgca agcgtgacaa
     1081 ggcaagctgg gcttcttttt ttgttttgta acctcgtcta cgaaccttat caggtttgcc
     1141 agtcattttg ttcattccaa gataggcttc atagagccaa aatttacctt ttttagtttc
     1201 atattgcttg aatgttgcca taatagtttt ccctccatag tgtagcaagc cgtgggggcg
     1261 gttggaggag gcagtttatt attattttga atagtgttta tttagctcag taacaagagc
     1321 tgtgaactct tttagaagat cattttcata aaattcttga tactcttttt tctcatctgt
     1381 atttttttct gaatcattaa ttaatgagtc atatcgtaaa agcgtattta gaagagctgc
     1441 gagataagca attggggtac tcccgtcagg aaattcaatt cgagatcctg ttttaagata
     1501 ttttattgcg tttgttaaat aaatcttctg tgagacagtc aggttatctt cttcgatgtt
     1561 attgatagca ttagaaagat tagaatcaat tatagagtta tttttttcaa tagttttttt
     1621 caattgcttt tcaagcacat ttcgcttaat ttcgggcatc atatgcatat ctcttaatgt
     1681 ataagatccg tttaatagat aggtagtgga tacatctaat acctttgaaa gttgtttaag
     1741 cctggtgtta ttgggaagat tatagccatt ttcccaatta ctaacggcac ctttactggc
     1801 cggtggggat aatttacctc caagttgttc cattgagagt cccttttcaa ttctagccag
     1861 tctaattctt tgtcctgtct ccactgcttc ggggtatttt tttctagcca cattttcacc
     1921 accattcata tattttctct actatatcac gatttaaata tgtgtgaaac ataaaagttc
     1981 aaaaaagtat gttaaaattc ttgcaaatga aaaggtatga tggtatattg agtttataag
     2041 aggtatagaa atgtattcaa gagcctcttg cattttttta aaatctttgt atagaaatgt
     2101 atgctaacga ggggggataa atatggaatt aaaaattgac aaactcacgc ttgatgatcc
     2161 agcttttaaa attattgacg atcgagttgt tcaacaagta gagcaaactc aaggaacgtt
     2221 actttcatta ttagaaaaaa agtaccagtt tccaaaatat atgaacaagg cacaagctgc
     2281 taagtatatg aacgtttcat acaatactat gatgcagaaa tatgttccta acggtttaaa
     2341 gttgattata gttgatggag tcgtaagaat ttcacaagat gaatgtgatc gctttatgga
     2401 agaataccaa aaataaacgc aagccgtggg ggcaaaggga ttaataggag gaaataataa
     2461 tggaatcgac acaaaataaa gcaatcgaaa aagtattatc aacaattctt acagaagaca
     2521 cagcggcaga attagcaaac ttggaaggca aagcgctaga agaaacgttt gaatgtttgt
     2581 acgaacaaat ggactatcaa aaattgctac cacaagaacc aacggtaagc ggagtattgc
     2641 gtggcttgaa cgatttagta caagcagagt ttaaagaacg actttctatt gaagagtatc
     2701 aagaaatttt gtatcagcaa gttgagttgt tagcgagctt gctagggctt gaactggagg
     2761 acgttgaatg aaaactgaaa tttggaacgg acacattata agatttgtcg atattaatga
     2821 tgaatggtgg gcagtggcga aagatgttgc ggaagcgtta ggtcttaaac aggttactag
     2881 agccattcac tctttgccta aagatggggt tactactagt aaggtcatcg attcattggg
     2941 cagaacacaa gatgtaaaca tcatcaacga aaaaaaatat ttaccgcatg gcattcaaaa
     3001 gccgtaaaaa agaagccgag gcatttcaag actggatttt cgacatcatc aaagaactac
     3061 gccagtccac aggacttgaa ggctttcaag tattccgcat gctagacaaa gaacaccaaa
     3121 aagaagcaat gactaaatta agccacgcta ttactgaacc aaaaccagta gactacatta
     3181 aagcgaatgt gatagccaat aaagctgtat caactattta tggtcattcc aaaatggtaa
     3241 aaaagaaaga tatgactcct gaaatgttgg ttgatcgtga acctatttta gatgagacag
     3301 tagaattaat gactgttaaa gaaaaatatg ggttgcagtt ttcagtgagt gagaaaattt
     3361 ataatcgttc tgctgaattg cagacaactt aggagcatta acatgatcga gaacaaagaa
     3421 aaagccttat ccataacagt cttggcggac aggataaagg caacttaatt gggataaaac
     3481 ccgatattct ttgttccgat tataccatat taaaaaatcg gagggaacca ttatgaaacg
     3541 aaacttaatt ataggaagca ttttaatcgg cggaattttc ggcttattac cattaattgc
     3601 aaaaatagct atctttgcag gattagcgtt atatctcttc actgcttatg acgagtacga
     3661 ctatcaatta agaactggag ggcataagta gatgtacaaa acaaaactat taaaccaact
     3721 cgatagcctt gaattagagg aaattaatca agggatcgct gaactagaaa ataatatcgg
     3781 gaaaacctac tttggaaatt cattcaatga aaaactaaca gttttgtatg tactcaaaaa
     3841 acatgcagaa cacaaaataa tttgtcgaga aatcaacgag ctaaaaaatc agattttaac
     3901 cgcatggtta aacattactg atatgcaaga agcaagggtg aaaacattta atacatgggt
     3961 aaagtatcag aatcaattaa aaggtgctga atttgttcgg gatgggttga aatacgaact
     4021 tgagcagttg aagctcatgg aggtgtctga atgatttata ccagtatttt gaaaaaggag
     4081 gagaggttta ttggcaaata attatcaagc agcaacggaa ttacaaaagt cgggatggtc
     4141 aatttatccc ttatctcctg gaactaaaat tccaataaaa ggctctagtg gttggaatga
     4201 tgcaactaac gattctaatc aaattaatga atggtggacg gaagatgcaa ctagaaatat
     4261 tgggttaatg cttgaagatc atcaaatggt ggtagttgac ctagatcaac attctgaaac
     4321 acgaaacggc gttgagaatt ataaaaaact tgcttcaatg tatgatccat ttccttctac
     4381 ttatacggaa atcacaccag gtaaaggaat ccatttcttc tttaaaattc ctgatggtgt
     4441 aattatccaa caagaaacag gtgcatttgc tgatatcttc gggcgtgatg ataaaggtaa
     4501 aagtttaagc gggattgata ttatcactaa aggagttcca atagcgccaa ctatgattat
     4561 ttcaagaagt gttgggagag catatgaacc tattgaaaac gtatctctaa ataatattgc
     4621 accagctccc gattggctag tgaacctact gctaaaaaaa ccgcaagaaa taaagactca
     4681 ataccgaccc gtagcaaaaa cagggacagc aaaaaaatta gatatgattg ttcaaggcgc
     4741 tggcgaaggt agtagaaacg atcatttaac taagttatgc ggttggttac tatggcacgg
     4801 tgtagataat caaactttag cagaattgat ttatacagca aatgcgtaca atgaaccacc
     4861 gctaaaagat aaagaagtga atcaaattat tcgatcgatg attaaaaaag atttgagggg
     4921 gaaagctcat ggctcataag cataaattag atagtatttt agactttccc gaagccagcg
     4981 agcgtgaaga caatatcatt gaattgaaaa cctggatgtc acgattaaga tgcaataaag
     5041 acgatcagat taaaagtaac agtgttgtga atgcagaatt gattttaacc aatgatagca
     5101 atttagctgg aacaattgct tataacgaat ttagcggata cattcaccta ttaaaagatt
     5161 ctccttggat caatcgtacc gctggagaat gggaagacag ttttgaggat gccttaacgg
     5221 catatattga agaaaactac aatgtagttt ttgatgacaa caaaatacac aaagcagtag
     5281 taaatgtggc tagaaagaat gttttcaacc cagtaaaaga acgtatcgaa aaagttaaat
     5341 gggatcaaca gcctagactg gaaacactgt ttattgacct tttaggggta gacgacaacc
     5401 tttacacacg agaagtaaca aaacgttgga tagtcggatc agtcgcacgt atctataaac
     5461 caggaataaa gtttgagatc gttccagttt tggatggacc gcaagggatt ggtaagagta
     5521 ccgttccagc attactttac acggatgatt ttttcaccga ttcattagat tcgctaggtg
     5581 aaaagaaaga tgattatatg cagcttcaag gcaatgtaat tattgaactt ggagaattat
     5641 cttcaatgaa caaaactaaa attgaacaga taaaaaactt catcagtgcc aagatcgata
     5701 aaattagacc tccttatggt cggaatgtca tagcttgggc aaggcaatgt gtatttattg
     5761 gaacgtcgaa tgatgggcag tatttgaaag atgatacagg aaaccgccgt ttttatcctt
     5821 tgccttgtaa gaataagcca aagatgaatc cgtttaaaac aaaggatgaa tattttcttc
     5881 aagtgttagc tgaagcaaag gtgctttttg ataaaggaca acgaatctat tttagtcccg
     5941 atgaagataa agaagtctta gaaattgcaa aagactatca agaggatgcc aagatggaga
     6001 atccaattaa agaagcaatc actaaatatt tggaaatgga aatcccgtat aaatgggaag
     6061 aagctccagc gtgggcgaga cgttcttatt atcagaacta tcctgacagt aaatgtaatg
     6121 agaaaaattt acagcatttc tataatggag ctactatgca acaagagttt tatttagttg
     6181 atagcgtact caccgctgat attttggaag ctgtttttga taaacaagca aaggatttat
     6241 tgaatggtcg atcagatgca gaaactaaaa agattgcctt gattattagc agtattcctg
     6301 gttgggaacg gaagcaactg ccaaaaagaa ataaacgacg aggattttac aataagtcaa
     6361 atgcctatga aaataaaaat cgagctggaa agtacaaaaa gtaacgggta gtaacgggtc
     6421 agtaacacct agcccgttac tagtttaccc ttactgcccc aagggataca gcaatttggt
     6481 aacggggtaa caccttattt ccttaaaact tttgaaatag ttagctaata tgctggggaa
     6541 taatgttact ggattttacc cgttacctgt taccaaaacg ctagaaccct tgtccccgtt
     6601 gggattgagt ggtaacgggt acctgttacg taggtgttac cacgtgttac gaatcaagga
     6661 ggaaagtaaa tgctcaatat tatttcaaca aacaaagcac cgaactttca atatacagat
     6721 gaaatggatc ggtttctaat gaacacgctg gcatttagtg tgggattggt aacagaagat
     6781 tattccacat ttgatccaga agtattaaag ataatggaag aagaacctga ttggctgcag
     6841 gagtcagtag cgtggtgtca atcgctagta gtgggatcat tggtagatag tggcaactat
     6901 gatgatacag gtgagctaat ggatgaattt aattgtctgc ttaacctata tgatcgagcg
     6961 agacaacgag agcttacatc gaatgaggat aatttgtttt taaacatcca tgataagttc
     7021 ttggctttgc tactaacaga tgatgaattg ataactaatt tattggaggt agaataatta
     7081 tgcatatgga aagtattaga tacttcgatg gtgagaaaca cattgagtat tcaaacactc
     7141 aagaagatgt agactttatt agttttgagg aagataaaat cactaaagtt aattttaaag
     7201 acggaacgtg cttgaagata gtttcgcctt atattgaata caaatcaaaa tgggaacaag
     7261 atgattctga cgaacgagat cgctttggtt ggtaatctat ggcagttaaa aaacaatgta
     7321 atcatgctgg atgtaaaaca ttgattgatt ataggcagaa gtattgtggg aagcataaag
     7381 caaagcaaac ggcaattaag cgtgaagaaa gaaagcaaag cgaaggtaaa tattttcaat
     7441 tctatcaaag tagaacatgg cgaaaggctt catacttgta tcggttaaat catccagtat
     7501 gtgaggattg cctagaagaa ggcttgataa ggaaagctga tgttgtagat cacaaaattg
     7561 aactaaaaga tgattggaat agacggttag atgaaactaa ttttcgttct ttatgccatg
     7621 cacaccataa ttccaagaca gccaatgaaa gacaaagacg agaaaagagt accctttgag
     7681 ttaagggggc atagtgtgaa acttactgac aatcgatgcc tactcatttt ggtacaaata
     7741 accgttgtga aaaggcataa gggtaattac aaataacgat tacacttgta atcacaagtg
     7801 aaaaagtata taatgaaagt aggagattta ttgaaaaata caattgttat tactgataaa
     7861 acagggaaac agcgcaccat tgagttacct cattttggtc aagtgaccat tcaaatgcag
     7921 aacggaaaaa tcatttatat agataaatta gataaagaaa aattctgatc gaaaaacgaa
     7981 ggaatgtgag cttaattgct tacgttcctt tttcttttgt ctgaaaggag gacaacatgg
     8041 gacaccctaa aatattacac gatacaaaag gcaatatatc aagtgaagaa aaagcaatcc
     8101 gtgtggacgc tagagaagaa ttgttcaagc agcaaccatt aataaatatc acgccccctg
     8161 actggatggc agcgagcgct agaagtgaat ggaatcgtat cgtaccaaca ttaaaaaaag
     8221 attatccatt gagtgaagca gattatggtt cattggtagc ctattgttta gcctttgctc
     8281 gaatgaaaac agccgaagcc gagataagaa aatcaggtac gtttatcaca tgcgaaaacg
     8341 gagtaaagaa agccaatcca gcggttcggg ttcaatcaca agctatgagt gatttgaaaa
     8401 aacaagccac ctcactaggt atgaccttag aatcacgatc aaaactagct ttgaacaagg
     8461 ttaaaaatga tgaacccgaa gacccgttca aagagttgat gaattcatga atgattacat
     8521 tgagaaagtc ctatcaggta agctgattgc acccaagaaa attatccaag cgtgtgagcg
     8581 tcatataagc gatttggagc gttctaagtc agatagcttc ccttatgtgt tcgatgaaga
     8641 acaagccacc aaagcgatta aattcattga gttgctacca tctacggacg gtaaagcaat
     8701 caagatgtta ggatttcaaa aattcattct aggcagtctt tatggctggc gtactaaaga
     8761 agggaattac aggcgattca atcgagcgtt taccagtatg agccgtaaga atgggaaaac
     8821 gtatatcgca agtggcatgg ctgccaatgc gttgattatg gaacaagaac cagcagaagc
     8881 aaggcaagta ttgtttgtaa gtaacgcttt gaaacaagct aaattgggct atgatatgct
     8941 gtctaattca cttagaaacg tggtcaagtc tagtaagttt ttaagaccac aactgaaaat
     9001 tatgaactct aagattcagc acttgccctc taattcgttc gctatggcac tggctagtga
     9061 aaccagcacg ctagatgggt ttgcaccaac aaccgcaatt cttgacgagt ggcacgaagc
     9121 aaaaactcgt aaaacgtaca acgtcattaa gtcaggaatg acccaacaaa agaacggctt
     9181 attgtgtgtt attagtaccg ctgggcttga tttaaacgtt cctatgtacg aggaatactt
     9241 attgttagat cgtgtgctaa aaggcgaaga acaagccgac aggtacttta tagcgatatg
     9301 ggaattggac gatcccgaag aaattcacga tcaagagaaa tggatcaaag ccaatccgat
     9361 ttttgaaagt gaagaaatca aaaaagtaat gattccaacc attcaagatg atgtgaacct
     9421 tgctttgaaa caaaataacc ttaattctgt attggtgaaa aacttcaatt tatggagaca
     9481 agcgagtgag gacagctata tgattgctga agactggcag gcaaccgaag tagaaccaca
     9541 agatattaca ggcaagcccg tttatatcgg agtggattta tctaaaacag atgatttaac
     9601 tagcgtttca tggatcgtac cgctagataa cggcgaactt tattgtgatt cacatagctt
     9661 tgtagccacc aaatatgggc ttcaagacaa agaaaagcgc gacggtttgc cttatcgaga
     9721 actagaaaaa gctggtgagt gttccattac tcaattagaa agtggaatcg tggactatga
     9781 ccaagtattt cagtttattc aagatttgat tcaagaaaat gatttggaat gtatggggat
     9841 ttgttatgac ccgtataacg ctaattcgct tatcagtaaa gctgaaaaag ccaactaccc
     9901 aatgttagaa gtaagacaag gaacgattac tctaaacgtt ccgacccgaa cttttagaga
     9961 acaagtttat gaaggcaacg ttattcacaa taaaaatacg attctcaccc atgcagtgaa
    10021 caacgctatt ttaaaaacgg ataacaacgg cattcagatt aataaatcaa agaacagtaa
    10081 caaaattgat ccaatagctg cattaatcaa tgcctatgtg tttgcaatgg attacttcac
    10141 cacaacggaa ggagcgaaag cagacaatga attttataca agtgaagaat tttctttcta
    10201 attacattca taccgttctt ttacttcttg gattggtgtg tgtgttggtt gctatcacct
    10261 tactaacaaa tgtctactat ggcttgttag cgctgggcat agtgcttatt ggggtagcgg
    10321 tcatgctaaa tacagaacag aagggaggtt aaaagatggc attttttaaa gcgagacaaa
    10381 atacaacggg agatcctttc ctacaacatg tggtatcaat ccaatcggat gattacacca
    10441 ccagctttac aagcgttcgt gcattaagaa atagtgatgt gtttgcagcc gttcgaatta
    10501 ttgctagtga tattgcttca agtccgattc aattggtcaa aaacaatatg ccacaagctg
    10561 ataatgaact ggtgaagtta ctaaacgaga aacctaattc agaaatggat ggttggcatt
    10621 taaagtttgc tttggcggtc aatatgttgc taaacggtaa tagctttgca gagattaaac
    10681 gcaacggtga aaaggtagaa gaaattcact tattacctaa ctcaagtgta acggttactc
    10741 aattagataa tggtacgttg tcttatcaga ttggtgataa aaaaagacgt gtgaagtcta
    10801 gcgatatttt gcacttcaaa tatttcaccc aagatggttt aacaggatta ccaccacttt
    10861 atgcgttacg tgatgaactg aaaattcaac aagctggcaa ccgcacattg cacaatttct
    10921 ttactcgtgg tgtcagtggg tcaggtattt tgaaggttca taagtcagat ttagacggat
    10981 cagcaaagaa cgcaattcgt gaaaagtttg aagaagccaa cggttcaagt agtggagata
    11041 atgctcttag aacgatcatt cttgatgaaa caatggacta caaaacatta gaaataaaca
    11101 ccgatgtttt gaagcttatc aattccaatg attggaacac aaaacaaatt gccaaggcgt
    11161 ttggtgtacc gattgagcgt ttaggcgttg aaaatgaaca ttctagcacc gttcaaagta
    11221 atctccagta tatccaaagt acgctgattc attactttaa tgtgtttgtg agtgaattag
    11281 atacgaaact tgaaaccaat atccgcttta attccgatca gttactagag actgatcctg
    11341 aaacaaaagt aaaaaatgta ttggatcagg tcaaagggtc acttctcacg attaatgagg
    11401 ggcggtcgaa aatggggcta ccccccatgg atggtggcga tcgtttacta gcaagtttga
    11461 actttacgta tttagatacg ttggagaaat atcaattaaa agaacaggaa ggagttacac
    11521 cagttgaata atgaagaaga aaaggaaaaa cggctgacag aagaagctga gctaacagcc
    11581 gattctccca aagtagagaa agaaaatgaa aaacaaccaa cagacggcaa aactatttca
    11641 ggctatgcgt tgaaattcgg gcaaccgtca aaagatttag gcggctttgt agaagtgatt
    11701 acacccgaag cattaaaaga ggtggattta tcaaatgtgt tcttattata tggacacgat
    11761 tattcaaagc cactagctag tgtcaaagca ggtacgttga aattgaatgt cgatgataca
    11821 ggattacatt ttgaagcaga gctaaccgac acgacctatt caaatgatgt gtatgagaat
    11881 atctcaaaag gggttgttga ttctatgtcc tttgggttcg tgcttggatt agattcattc
    11941 gacaaaaaag aagatggcac aattgaacga tcaatagata aaatcaaagc acttaatgaa
    12001 attagtgtcg tgaccgttcc cgcttatgat tcatcaaatg tccaagtaaa taagcgttct
    12061 tacgaatcgt ttatgagtaa caaccaagca aaacaaacaa acaatagctt agaatccact
    12121 tctaaagcac aaaaggagag taataacatg gaaaaaactt taatcgataa cgaaaaaaca
    12181 gaattgcgtg ggtatgaaga atatatccgt tcacaaggcg aagtgcgtga tggagtcact
    12241 actgtaaatg cagcggcagt tgttcccgaa gaagtaatcg gtgaagtctt tgatttgaaa
    12301 cgttcaaatt ataacttagc tcaatatgca acagtaaaaa cagtatcaaa tggacaaggt
    12361 aaatatccag tagcaactaa ccaacaagca gtgttagcaa caaaagctga acttgctgaa
    12421 attggtgata ttgacgctga aatgtttact tcagttgatt ataaagtaga aactcgtgct
    12481 ggtaagattg ccttatcaaa tgaggttgtg gaagattcag cagtgaatat tgtacaagag
    12541 gtcaaagatc agttagcgaa attggtagaa aacactgaca ataagcatat catggattta
    12601 ttaaaaacat tcactaagaa aacggctgct acgttggacg atttgaaaca actatacaat
    12661 gtggcattag acccagcatt aaataaaatg gtaattctaa accaaagcgg atattaccac
    12721 ctagatacat tgaaagattc agatggacgt tacattttac aacccgatgt gacagcacct
    12781 agtggtaaat cattattcgg tatgccagta gtattgattg cagatacatt gtttgccaat
    12841 cctaaagctg gaacattccc tatgattatg ggggatattg cacaatctat ctttgttgct
    12901 cgtagaaatc aagtaacgac tcaatgggaa aaattcgatt actactcaca aggacttgca
    12961 gtgatcgttc gcaacgatta taagaaaatt gatcaaaatg cttcagtgta tattgagttt
    13021 acgccagttg taacaccaaa agtatagaaa aatattgggc ggcggtttat cccactgcct
    13081 ttttttcatt aggagagata aaatggtaat tttggatagc ataaaaaaaa gtatgcggat
    13141 cgatcacact attgatgatg actttattca acaattgatt gatacagcag gcgaatatat
    13201 aaaaagtgct attgatagta gcgcaacgga taaagatatg gataattatc agcaatttga
    13261 tttggcggtg tcattactta ctcaacattg gtatttgaac cgtcaagaag ccagcagcga
    13321 acggatacca gtaacggtac aagcattagt acaacaaatg agaggtgctt attatgccaa
    13381 tcattaagaa tgtaaatgaa ttgactgaga gagtcaagtt taaaaagaca aaacgggtaa
    13441 aagatgaaga tgggcagatg gtggatagtg aagaaactgt atttgaatgt tgggctaatg
    13501 tgcgttcaca aatgctaaaa gacgtgcttg ctagtgtagg taccattctt gaaggaacgt
    13561 tgacgtttat tattcgatac gatcaagatt atgaactaac aaacgatatg aaagtagctt
    13621 ggaaaaacaa gagttataaa attatttcaa tcaatgaagg aacggcgttt aaagattata
    13681 caacgatcat agctaaacag atttcttaga atgattacac ttgtaaacgt tgtgggtatt
    13741 tagtataatt aaggtagtaa atgaagggtt agctgcctga attttgcaag acttagctag
    13801 tcgaaatcta ttgttggact gaaaattgta ggtgtggttg caaacatgct ggactaagaa
    13861 agatgataga tgatgaacgt tcaattttaa tacaggcatg ctaggactga ttggatggtt
    13921 ttggcaagct gattgtgtag aaatcaaaga acacgtctgt tgtggacgtg ttttttttgt
    13981 tataattcaa aagaggtgat ttaatggaca aagcagagtt tacaaatttt tattggaagt
    14041 attatctaca tttagaaaat gagtttatta ataccacaga ttttgtgatg ctcgatgaga
    14101 ataatttcaa aacattctct attgaatatc aaaaactact actagcgatt ggttcggagt
    14161 gtgagattat tttcaaagaa ctttgcggat ttgatagcag tagcaataaa agcataactg
    14221 attttaaagc tgttattaaa tctagcgaat taatgtgttt ggataatggt gttagggtta
    14281 tgaattcttt aacattgact tcactaaaac cttttggaga tgggtggcct gaagatacac
    14341 cagattggtg gaaaatatat aacagagtaa aacatggtcg ttcttcaaat tataaggaag
    14401 caaatttgga aaatgtgttg tatgctttag caagtctata tttattagag gaatatttgc
    14461 ataagaaagt aatactagaa ggtgaagtag actttataag tccagagtca aatctattta
    14521 cgttatcttg ggaaagaaaa cattcgtcga tgcaaaaagt gacgtttgaa aaagtggatt
    14581 caaattatgt agctccactc attgatttta aagaacaaga agaaatttaa aaacccccac
    14641 ggcttattga agtttttgta tgccatattg tatgccattt ggtttaaatc gcttgaaaaa
    14701 tagtgataga taactctaat tcatttagtc ataaacgctg ttttgataac ctttatagtt
    14761 ctttgatatg ggtttctcat tcatggcgga agaagaagaa tagaacaaaa gaatgaatta
    14821 ataagatata aaaaaaggga ttgtgacaaa cgtcttgtca caatcccttt ttccaaataa
    14881 acgatgttca atcagctgcc cctcggcatt cgctcgaaaa acggcgaaat atgaagaact
    14941 attttgccat ttgtttctct aataatcagg tcaaaacgtt gacttcacag tctcttcctt
    15001 aaacggtgtt ctcctgccaa ctcctcggtc acaagtcaca atattcaaca aacatgagaa
    15061 gctgtttttt gaaattgttc cttgtgactc ggagctgaac ggcaggctca cgacctcttt
    15121 cttaaacggt gttcaaaagc ttgcatctgc ggtaataagt tcaaccaaac gaaaattatg
    15181 aggagctatt tttgtttggc tgttcttatt acttgatgca ggacagcttt ttcacaacct
    15241 cttttcgttt agcactaaat ttccttaaaa agtagaaaag ctttcgaagc ctcgtttgtt
    15301 tcttttactg tataataaaa gaaaagagta acaatcgcta aaagatataa acaagagaaa
    15361 ggaaatacaa gatgattggt ctgatttatt ttgtggtgat cgttctggcg aacacagttg
    15421 gcgctatttc cgggatgggt ggaggcgtgt tgatcaaacc gattcttgat cttatcggcg
    15481 cccattctgt tgcagggatt tctttttact caacagttgc ggtattcacg atgtccattg
    15541 tttcgactgt gcgacaagta tcaagcggga aatcactgaa ttggcagatt gtcggttggg
    15601 tatctagtgg agctgttgtt ggaggagtag tcggcaacat tgtattcgaa gtttttttac
    15661 agttatttga aaacgagaag catgtgcagc tgatccaaat ttttttgacg gtattgacct
    15721 tggtttttgc gttcttctac accaagcatc atcagccaaa atttcatttg acgtcatgga
    15781 cttggtacct tatatgtggg ggggttctgg gctttttagc tagtttttta ggtattggtg
    15841 gtggaccagt caatgtttct ttgttgatgc tgatgtttgc attgccaata aaagaagcaa
    15901 cactatattc tctatctacg attttctttt ctcaactggc aaaattggta acgattgctt
    15961 taacaagtag ctttatgcgc tttgatttat ctatgttgtt ttatgttatc cccgctgctg
    16021 ttattggagg tttgtgggga gcaagattta gcagggtttt gtctccaaaa aaagtgacct
    16081 ttatttttca agtaattgtc ctcgtggttc ttttgatcaa tctttacaat gctttcgtca
    16141 tcttacaaac attaggagaa tctagactaa cgcaagtcaa aatcactgaa acatattgtt
    16201 ctaaatgaaa aaaggagtat actgtgataa aaaaattcat ttaaaaaaat aaaaattggc
    16261 tggtgatttg gatgttgcgg gaaatgcgtt taggagatgc agaagcacta cgagaaatca
    16321 atgcggaaca attaggatat gatgtaccat taaccgttac ggtgagacaa atgaagaaac
    16381 tgttgaagga gccagaaaaa aattttttct tggtctatga agatcctact tctaaaaaag
    16441 cagctggcta tgtacaagcg gagtcctatg aatcgatttt cagcgatccg atgttcaata
    16501 ttatggcact tgctgtgtct aaacatgctg aaaaaagagg aatcggaaag gccttgatga
    16561 ctggcgtaga agaagcgaaa agacgaggaa tatcagcaat aagacttaat tctgcagaat
    16621 acagagtaga agcacaccga ttttatgaac gcatcggtta tcattccgat aaaatgcaaa
    16681 aaagattttt gaaaatcctg taaattttta tcaaaaaata cctggtcatc aagccgatac
    16741 ttgatgatca ggtatttttt ttgactaaga aagtaattgc atcaagaaac ctgagatgat
    16801 caaaacgatg gcaccaccaa gccgattgcc catttgagca aaagcaataa gttccatccg
    16861 attagctgca gataagacag ctacatttcc tgtaccaccc atcgagttgt tgcataagcc
    16921 agcagtaata gttgattcga ttggatataa accaaacaaa cgaccgacaa atcctgaaac
    16981 tactgaaatc gcgataacac ttgtcaaaca taagacaaca aattgccatg tcaaagcaga
    17041 agccaataca tttagattca ataacgcaat accgataccg gcaagaacag ctgctgtcaa
    17101 gtttttgaca atcaagttgt tgaacatgat agctgcatct tcgtaatatt ctggtaatag
    17161 gtttgcgatt ttacagataa caacgataat gatcatgaat gcgtaagcat ggactttagg
    17221 aacaaaatag ttacagattt gtcctaagat gaagaaagtc aatgatacca tcaaaccgac
    17281 acctaattgt ggaatggaag gttttacatt tctaggtttt ccatcgccaa ggtcgccttt
    17341 ttccattaat ttaccattac cgttgaattt tggcattgat tgtcccgcac gagcaactaa
    17401 cgctgcacca atgatagcta ggacatttgt cattgctgtc gcaggaatca ggcgggaaat
    17461 gatagaagaa gcgtccgtac ctaaaacatt tgcataaatc gttgataaag gtacagctcc
    17521 ggctccaaca ccaccagcca ttgcgggcaa tgagatatat aaaacggaat ttgcaaaacc
    17581 atttccgata agcataccta cgagtcctac ggcaaaaaag gagacgacca ttgaaataag
    17641 tgcaacggga ataaaacgaa cagaagcttt catcaataga ttacggttca taccaaggat
    17701 gctaccagta atcaaggcag caatataaaa gtctaaaaag cccatattgc tgacaaagtt
    17761 ttctgtggat tttactacat actcaggtaa gatcccaaat gtagcaatcg cggcagaggc
    17821 aaagatcgca aaaacggagc caccacctaa ataacttttg acgataggga tttttgttcc
    17881 gataaaatga aataaattac cgaaaatgac caaaacagcg attgggccaa tcatattcgt
    17941 cggtaatttt cctaaagcca ttgcaatgat caggacaagg accatgatca aataaagggg
    18001 caaacttacg ccggtaattt ttgttgacca aaacccttct tttttctttt cctcaactgc
    18061 agttgcggtt gttgtttttt gtgccatttt tacagatcct ttcttattct gtatcgtatg
    18121 aaaaaagcca aagagaagcg gcaaaagagc agtagttggc tggctgctgt tttgccgcag
    18181 tcgctttttt ctttttagac gtcatacaaa gtttctgtat gttcttttac tataacaatc
    18241 ttaatcttct aaagatttat attcaggaac ccatttagca gattcaacag cttctttgat
    18301 atccgtgatt tcttcacgat tcaatttttg tgcaacaact gattttgcga cagtttcagc
    18361 aatcgtttga gagaattcag tgatttttgc tactggaggc aagatagctg ctcctggttt
    18421 tgttacatca acgattccgc ctaatgctcg actagcttga gaaatgattt cagcatttac
    18481 tcgtgtagct gttgaagcaa tcaaaccaag ccctaaacct gggtacatca acgcattatt
    18541 tgcttgaccg atttggtaag ttacaccgtt atattccaca tcagcagcag ggatacctgt
    18601 tccaactaaa gcttttccgt ccgtccattc aatcaaatct tttgcttttg cttctgccaa
    18661 ttttgttgga ttagacaaag gcatgatgat tggacgtggg gtatgagccg ccatttcttt
    18721 cacgatttct tcagtgaatg ctccaggttg ggtagaagta ccgatcatga tcgttggatg
    18781 gattgctttt acaactgctt ctaagtttgt caattcatca gcattagtga attctgagcg
    18841 tttacgagcg aatgggattt gaccaggagt caatcctttt gtatcttcaa acaataaacc
    18901 ctgtttatct acttgataga aatgttggcg tgcttcttct tcaggtacac cttgacggat
    18961 catttcatct aaaaggatgt ttgcgatacc tacaccagca gtaccagcac caaatgtcaa
    19021 gatcgtttga tctttcaatt gctcacctga tatattcaat ccacctaata caccagctag
    19081 tacaacgatt cctgtgcctt ggatatcatc attgaaagta gtgattttat cttcgtattt
    19141 ttctaagatc gttgcagcat ttcctcgtcc aaaatcttcc caatgtaata acaattctgg
    19201 gaataattca gtagctgatt gaacgaattg atcaatgaat tcgtagtaag tttcgccttc
    19261 tacacgagca tgacgattcc ctaagtataa tggattattc aataattctt cgttattagt
    19321 accagcgtcg attgatacag gcaagacttg tgcaggattg ataccagccg cagcagtgta
    19381 gaccatcaat ttaccgatag cgatgtcaac accgttcaca ccccagtcac ccatacctaa
    19441 gattccttct gcatctgtca caacgatcaa acgaatgtcg cgtccatctg ctgcgttttt
    19501 caaacttgct ttgatatttt ctggttcgtc gattgaaaga aatgcagcat cttgtggttt
    19561 caagaaaatt tcgttgtatt gttcgattga atccgcaacg acaggatcat aaacgacagg
    19621 catgaattca actaaatgtt cgcccatcaa tttataaaac aatgtccggt tcgtattgaa
    19681 tagattcatt aagaagatac gtttttccaa atcggtctgt ttgcttaagt attgaccgta
    19741 agcttggaca gattgttgtt ccaatgtttg gacagtactt ggaagcatac cagtgattcc
    19801 gtattttgcc cgttcttctt ttgtaaaagc tgttccttta tttagaaaag gattatttaa
    19861 taagtttagc cctgtaagca tgaatgattc ctcctaagtt ttaatacaaa cgaagtatag
    19921 gagtcagatg aggatatagt caaaaagagc aggtataata aattttattt atatgagtaa
    19981 tagagtcaaa agaaatcaaa atagcggtat ttgttctttt tatatcaaca atccttgctt
    20041 gattactatg cgtatctctt atatgctaaa gaaagaaaag tagaagaata aaggatatgt
    20101 tagatagata aatagaatct ctgaagaata aatcacgtga tcgtgacgat aaaatacact
    20161 tacgtcaggt gataaaaaat acataagtag aaaactaaga ggaggaacag aaaatgaaag
    20221 aagttcaatt gatcagaaaa agcgaactat cagaaggtgg ttgcaatgca tgcggcgtag
    20281 tagaagcaac aagctataca ttaaagcttg gtgccaacaa agcgataatc tctgagttga
    20341 cagtaggcgg tcttgtggac agtctggcgc ttgctgaagg ttttattgga gaagatattt
    20401 atgagatgtt ttcagaaatc cgtcagttaa aaaaagggga aaactgcata gaggttcatc
    20461 atgagagccc gaatgtgaga ttcaaacgtg gtgataatga gatgatcttc aataatcatg
    20521 tgtccgatca caccgagcta tatggaatcg tgaatcaaat actaactgag ctttttggat
    20581 tgggacctta tgcgttcaag gaagaaaacg gtaatccaaa attaaacgaa gaatggcaag
    20641 aaacgattga aatacaaaga aacaacccgc acttatttca ataggcatcc acagtatttc
    20701 gtaaaaggga ctttgacacg acttttgtca aagtcccatc gttttttatc tatttgtgtt
    20761 ctaaacattt ctttcagagt agtaacaact ggaatgactg aaatgtaaac agattatttg
    20821 cctgtatcaa tacggaagag taatataaag tgtgtaaacg ctatcatatt attcattcaa
    20881 ttttttatag agggaaggca ttgaaatgga aaaaacgtat caagcaggta caaatgaagg
    20941 aatagtggat tttatcaata tggaagattt agaaatagcc gcttcacaag tgattccagc
    21001 tggtggttat ggttacatca gcagtggtgc tggcgactta tttacttatc aagaaaatga
    21061 acgggcattc aatcatcagc tgattattcc tcatgtatta aaagatgtag aattacccga
    21121 tacaactaca cattttgaca aagaaacgct gactgcacca atcatcatgg caccagttgc
    21181 tgctcatggc ttagctcatg tgaaggcgga gaaagcttct gcaaaaggtg tagcagattt
    21241 tgggacgatc tatacagcaa gttcttacgc ttcttgtaca ttagaagaga taagagaagc
    21301 aggcggggag aaagcaccgc aatggtttca attttatatg agcaaagatg atgggatcaa
    21361 tttggatatt cttgaagtgg ccaagcgtaa tggagcaaaa gcaatcgtat tgacagcgga
    21421 tgcaacagtt ggcggaaacc gtgaaacaga ccgcagaaat gggtttacct tcccactgcc
    21481 aatgccgatc gttcaggctt atcaatcagg agtaggccaa acgatggatg ctgtgtacaa
    21541 atcttccaaa cagaagctta gtccaaaaga tgtagaattt attgctgctc attctgattt
    21601 gccagtctat gtgaaaggtg tccagtcaga agaagatgtc tatcgttcat tagaatctgg
    21661 tgctggcgga atctgggtat ctaatcatgg tggccgtcaa ctagatggtg gtccagcagc
    21721 gtttgattcg ttgcaatatg tggcagaagc agtagataaa cgtgtaccaa tcgtgtttga
    21781 cagcggtgta cgacgcggac agcatgtttt caaagcaatc gcatcaggtg cagatcttgt
    21841 tgctattggc agacccgtta tttatggatt gtctttagga ggaagtacag gagtccatca
    21901 agtgtttgat ttctttaaaa cagaattgga aatggtcatg caattagctg gaacacaaac
    21961 agtagaagat atcaaaaaaa taaaattgcg tgagaatcgt tttatttaag aaacaaatga
    22021 cgaaaaaaat aggagtgcgg cagatgatgc cgtactccta tttttatagt tcaaggatgt
    22081 cactaataga ataatttgaa acgaaaaaat aaaattgaaa tattttttca tacttaataa
    22141 aaaaggaaga atattcatgt aaacgatttt attgtttttt tctcttttat acaaaaaggg
    22201 tcataatttc aagtaagcgg atgaattcat gaggattttt tgcatactgt ctctactata
    22261 cttaggtcac taagaaaaag gagtgattta ttatgaaaaa gaacatgatt gggacattat
    22321 tactttcaac acttcttcta ggcggcttgg caacaccagc ttttgctgag ggtcaagcga
    22381 cttcaaaagg agatattacc tttactgaac caacaaatac cgtggagcct cttaacccta
    22441 ctgatccttc aaaaccagta gagccagcag atccggaaaa tccagcaact ggccagacag
    22501 gtcctttaac actggatgta gttcccgaat tgccatttgg tacacacgag attgaaagcg
    22561 gaacaaaaac gtatcaagta gacgcttcaa aaaacgatac accttatctc caagtatcag
    22621 atcgtcgagg tgtaggtgca gatggccaag cacaagggtg gaacgtgaca gtttcggttt
    22681 cagattttgt aaacggttca caagttttgc aaggcgctga attagatttt ggaacatcca
    22741 ctgttaaaag tacgtcagat aacgaatcca ctggaccaac tagtcaaact gtaactgggt
    22801 tgagtaaagc aagtgcagcg acaccaatat ttacagcagc aaaagatcaa ggtctgggaa
    22861 catggctttc tgtttatgat ccagcaaata tcacactgaa agtgccgaaa gcagcagctg
    22921 gaacattcac ggctgatttg acttggaacc tcgtggctgg cccagtagca tagagtatga
    22981 aataaattca acaaactgcg gtgaaaatac tataggtgaa ggatttttta ccgcagtttt
    23041 tgttgaaagg atgtggtgga caatgaatca aagtaaacga ctgtttgttt cgtttttttc
    23101 tattttaagt ctatttttca tttttccgtc aatcagccaa gcaaaggatt cagcaggaga
    23161 ctttggaatc aaaccggtat ttccagagaa ccaaatagat aaagcaatcg gttattttga
    23221 tttattagtg gcaccggaac aaaatcagac actagaagta attatcagca attcttcaga
    23281 tgaagaacga acatttgagg tctctgtcaa tccggcggta acgagtgatg gtggaacgat
    23341 cgattatagt caaaaaaacc caacgttaga tgagacattg ccgtttgatg tccgtgacgt
    23401 tttgctcatt gcgaaaaagg aaatcaatgt atcagcacat gcagaaacaa ctgtacctat
    23461 agaagtgaaa atcccagcaa aaagctttaa gggacgtgta ttagcgggta ttcatgtttc
    23521 acctaaagaa gaagctgaaa cagaaaatgc tacagaagga gcacaaatca aaaatcgtat
    23581 tgcttataat ctcgcagtcg ttttacaaga aagtcaagag acgatagaac cggatctgaa
    23641 acttttatca ggagatttgg acgaggtcaa cgcaaagcca actgttcagc ttcgtttcca
    23701 aaatcctcag cctaggatta tcagcaattt gatatttacg agcaagatct tttatgaaaa
    23761 tcagctttat attgaaaata cgtctaatgc atttttagtt gcaccaaatt cgaattttca
    23821 cttgaatctt gatttagctg gtgataaagc aaaagccggc gattaccgcg cagaaatcat
    23881 agcaaaatca ggcgacagta atgaatggcg atttacacaa aactttacga tcaaaaaaga
    23941 aaaagcacaa aaagtaaatg aaaattccgt ttttgcagta caagaacaat ctttcccatg
    24001 gctttatgca ggattggttg ctgcagttct agtgttatgg ttgattttgc tgtttttgtg
    24061 ggtccacaag aaaaagaaaa aaggagaaac aacatgatga ggtgatttcg ttgaaaacat
    24121 cgatcttatt gggagggatt attgcaagtt tgctgttctt cagctggcaa gagccagtcc
    24181 aagcagatac caaaagttca ggtacgatta cattttatga tctaagtcca attccgcatc
    24241 caagtggttc agtcaatggc gcatcaaaac agcagacaca gctaccgatt tataaagaat
    24301 ctaatcaaca tggcgtattc ccggagacga atgaacaaaa gatccgtttt ctttatttga
    24361 tcattggtat agaattatgg cttcttgcgt ggttgttgtt atggagaaga aaggaaaagg
    24421 aggatgaaca tgttgctaca aaataaattt tcattttttc gctccttctt ttttacttcg
    24481 ctttttgttt taagtatatt tatcttttca ccttcggcag aagctgttgg agcaacgcct
    24541 cctgccattt cttatgatcc atacacgtat gaaaatatcg tacctccaaa tggtggacgt
    24601 agtgcagtca atggcgggaa gcttgtccaa ctaactgggg ctaaccaatt gcctaaattg
    24661 actgctactt atttgaacga tggttcaaat gatccttgga aaggtggcac ggtccgtttt
    24721 cattgggaaa gccatgtgaa actaacaaat gtacttgatt tggtgaatat cactgcacaa
    24781 ggatttgcat ttgtggcgat catcaatttg ccggaacatg tatcaagcag tgatgtatta
    24841 aaagcaattg actggaacaa cgcctacttg tatattgccg gtgcccccat caaggttact
    24901 agcggaagtt tattatcagc aggaagccat accttgcgtt ttgggatggg gtcgtgggat
    24961 acaaatactc tattgacaga tattattgct attattacgc gaggcgaatt caacgtggac
    25021 gatatcccgc ttcaatttga tgttacagct aatgtcgcga agatgacaga aaacggagat
    25081 aaatatgata cgagtccaaa caaaattttg accaagggta aattacaacc ttctatgtct
    25141 aaaaaggccg ttttttctgt tgatttttat gattcgaata atattatcca agatcgtatg
    25201 ttcaattcga ttttagctcc agctgcaggt agattttatc ctaaattaac tggcggaaac
    25261 ttgaatccag gagcatatgc attttctgca agtgcagaga taaatacgtg gaatagttac
    25321 atctcaccaa gtgattcaac aggaaaatat aatacatttg ctgataccac agaatacgtg
    25381 gatggaaata accagaacct tccaggctct accaatagtc ggacattgac gatggggctg
    25441 actgaaggac gttttgatca gttgccattt agtcgctttg atcgcgtagt caactatttt
    25501 acaggaaaaa acgttacaca aggagcaata ctgactcata ctccagcaag cacaagtgga
    25561 ttgaatcaga cagccaatat cgtgtacagt gggaaagatg cgcaaggcgg attgctttct
    25621 cctgttgctt taaaggtatt ggagcagtac aagctagacg gaacggtttt accaacaaac
    25681 ttaacagccg atagtcagtg gggaaagctc ctttcttctg catcgtacca agtcacggcc
    25741 aattggtcag caactgaact aaaaaagggt tcgatccatt acaggcttta ttcgaaagga
    25801 acccaaaaat tagcgacagg atggacagat caacttttcc aaacgatcca atcaaataac
    25861 gggggtgtaa atacagctac ttctacacta cctgcattgg cttcagggca atattatttt
    25921 gattatcgct tggtggacga tgtacttact aacatgtatc cgtcttttgc gtacaaatgg
    25981 cagtctgaac aagctggaat caccactctt cctcaaataa cagtggctaa ttttccatct
    26041 gtgagtgcag atagtcagct aaaaaacctt agcagacaac ctgagacagg agatcccctg
    26101 tctgctctat caggagatac gattcaagaa aatgtgacat tcacgttaac aaaatctggg
    26161 gatgcgataa ccgacaaaaa aatcacgatc tcacttcctg aaaatactac ttatatcaat
    26221 ggttccttaa aattaaatgg tacaactatc gcagataccg gcattcaaca aggagtttct
    26281 gtcccagcag atctattgag caaaatagga gataccattc atcttactta caactatcaa
    26341 ttaaatactg tagatacttc tttacaatcc gtcagcatac tgacaaaggc agctgtactt
    26401 tccagcaata ttacattagc agatggtgct aagcttccta acccagttgt ccaaacatct
    26461 gctaaaacaa ttcttgtccc caaacaagaa ttaacattag ttaatgtacc agatgatttt
    26521 acatttggta acgatctccc caaaccgttg aagacaagtt attatgaatc aaaaggcgat
    26581 ttttcttttg atgtgcggga tactcggctt ccaagcacgt ctccttggca actgacaggt
    26641 acattgactt ctttatttaa gaatgatcaa ggtcaagaat taagcggaac aaaattatac
    26701 ttcaatcatt ctggatcaaa gcaattgatt cagcaaggtc aaaacacgtt gatttatgag
    26761 agtgatggaa cagctaaagg agaggttctc gttgactttc ccgataccga tggtttactc
    26821 ttagaggtaa atagcagtac gaatgctcaa cctggcgcaa cgtatcaagg catggtcaca
    26881 tggaaactga cagctgggcc aaccagctaa gtgattagaa tttgtatgga tctgtgataa
    26941 ctgtcacaga tcctcttttt caaggatgag aaggaaactt ctgctgttaa aatcagaaat
    27001 gcttgaagga gtggacttgg aacttttcag tttgcttgaa aaaaatgaac aacggcaaat
    27061 caaacttttc caacttttat tagaggaaaa aggagaggtt tccgttaata acgtacttac
    27121 ccgcttatca gttgatcggg ctaccttgaa agaagatctg ttttatttac aagctaactt
    27181 ggaaaatatc aaacaattgg aaattgttat ccaagaaggg aatattttat taatcagaca
    27241 tggaaatata gcagcagtcg atgtttatta tcattgttac ttgcgctatt ctacaaaata
    27301 tcagatactt ctttatctgt tggaaaatgg ctcatttgat cggcagaaac ttgtcatgga
    27361 acttaatatc agttcggcta ctttgtctcg cagagtgaag gaattaaatg attctttaaa
    27421 tgagtttcat ctgaaaataa aaagcgggcg gctgaccggt ccagaaagtc aaattcgtta
    27481 tttttatttc caattatttt ggtttggacg tccctatctt gaaaacaaaa aagaatacag
    27541 tgatttatca ggaaacactt ttttagatct gctgcaaaaa gagtttagtt ttgctattac
    27601 agaagaagga cgattgaaat ttgaaatatg gctttccatt atgaagaagc gaatgcgctg
    27661 tacgaaaaaa tccaaaccag attatagcaa tcttcctgtt ggattgtttc aaacgacaca
    27721 atttttattg gacctccata catttttgac ccgttacttc tttcaatatg cctttccttg
    27781 gaatcaaaat gaaacgttgg ttttttatct tttcatgatc agtaacttta cattagatac
    27841 acataactcg catgtccagg agtttttgat aagcgaaaaa tacaaagacg acgtagtagc
    27901 tcaattgaat cgaacgttta aacaatcgat agaagaatat ttctcttttt atgtttttcc
    27961 agaagagatt tcgcaaaaga ttgcgttgac agtgatgcaa aatcacgctc attttgccta
    28021 ttttagagga tggatgaata tttttggcca agaaggatta aaacagcggt taactgaagt
    28081 agaagataaa caattcctgg ttttatgtga aagattggtc agacaaacat ttgctattgt
    28141 tgattttact gaagtagacg aggaaaatgc ccgctttgaa ttatttgctc gctatgcatc
    28201 tattttacgg acgctgatca agcagttaga attacgtctg caaattgctt atgatcttcc
    28261 ttatgaaagg ctaatggcgg atatcgtgaa agaaatcatc caggaacaat tgctgaatac
    28321 gatcaaatat gatttagtcg aatacgagaa agaaaagcag tatgatatca ttttgactag
    28381 ccaattgaaa gagtacccgt cacagaagtc cgctaaagtt tttgtattca ctagcaacga
    28441 atataattat gatttcccct atttgaacca atttttaaaa gaatgttatt tagaaaaatt
    28501 gaatttaagg atgaataaaa catgaaaatc gtgatcattg gtgcttccca tgcaggcatc
    28561 acagctgccc taaatctaag aaaacttcaa ccgaaagcag aagttttact gatcgatgaa
    28621 gatcataaag atggactagg ctatgtttca aatggaatca acctttattt aaaaggaaag
    28681 atacgtagtt tagctgaagt tgcccataac atgcggactc ttcaagaaag tggggcaaaa
    28741 ctgatcaccg aatggagagt aactgaactt gatccagaca aacatcagct catcctcgca
    28801 tccaaagaag gaaaaaacga gaccataacc tatgataaat taatcgtagc gacaggttcc
    28861 tctcctgtca ccttgtataa acaaattgaa gctgaaaatg tctacaccta taaaaatcta
    28921 gtgcaatcta agcaagtact tgccgcttta aaagaagcaa aagaagtagt gatcttcggt
    28981 gctggttaca ttggattgga attagcagat gctttgagaa ataaaggata cattattcat
    29041 ctagttgatt atatgccgaa tgttttatca agatatttcg ataaggatat gatcaacagt
    29101 tttcaaaatc agctgcaaac taagcaaata aatttttatc caaatgaatt cttgatcgat
    29161 tggaaaaaat cagaagagaa agtagtatcc gtacaacttc tgtctcaaac aataaaggca
    29221 gatatggtca ttttttctgc ccaaactcgt cctaatacga cattgttaaa agaaaaagtg
    29281 gctttgtatg aggatgagac tgtgatagtc aatgaatatc tgcagacaag tgatccggac
    29341 atctatgcga ttggagatat cgtcccagtc agttttgaca aaaataaacg gcatctcttt
    29401 ttacctttag ttactcgagc tgtacatatg gcaagagcag tagcgctaac tttgagtggt
    29461 cagccaaccg cttacgacct cagacaaaag ataacagcag cagtcattat agactacttt
    29521 ttaggaactg tagggctgac agaagatgaa gcgccattct tggaacaaaa taccggttcg
    29581 tgttcaggag aatttgatct atttccacaa tacgacgaag aaaacaaaac agtaaatgca
    29641 aagctgatct accatccgga tacattagaa ataattggcg gacagctgat aagccaagaa
    29701 ttcttattat cagatttgaa cctgcttgcg gatatcgtga aacacaaaac tacgattcca
    29761 cagctagcag ttgaggattt tggatttctg tctgaataca cctctcgttt tcactacctc
    29821 aatgagttag cttttaaagt actagctaag agagcaaata gcaatagagt agataaaaga
    29881 ctgtgagaaa tatcacggtc ttttattgtg tggggatata ataaatatta atcaatcgag
    29941 aataaaaaat tcatataatg aaatagcgga agaatttttt gagcataata ttcgccaaaa
    30001 atttatcaag tgataaaatt gttgtataaa atgattattt caggaggagg agttaaatgt
    30061 ttttagcatt aaatgaaatt atgcattcaa agttacgtta tgcgttagtt gcaggggtga
    30121 tgtttttgat cgcctacctg gtattttttt tgactgggtt agcttatggc ttagctcaag
    30181 ataatcgaac agcagtcgac aagtgggagg cagattcgat tgtgctttcg aaagatgcaa
    30241 attcaaatct agggatgtcc atgattacta agaagatagc agaagaagtc gaaggtggga
    30301 aagtggcgta tcttgcccaa acacctggag tcgtgactag taaagacagt acagaagaag
    30361 gaaaaatcaa tgttagcttc ttcggtatcg ataaaaatca attcatcatg cccaatcttg
    30421 ttgaaggaaa agcttttgat aacgatgatg aggcagtagg cgatattagt ttgaaagaag
    30481 aatatggtct agccatagga gatacagtta agttatctgg tagtgacaag acgtttaaac
    30541 taactggctt tacggatcat gccaaattca atgtctcacc agttctctat acaaccatca
    30601 atgcttacca agaaatccgc ttcgaaaaag aagatacaag tgaaaatgca cggattaacg
    30661 caatagttgt cagaggtaaa atcagtaatt tacctgaaga tttagaacag atcaaaattt
    30721 ctaaattcat caatgaatta cctggatata atgcgcaagt attgactttt ggatttatga
    30781 tcggattttt gatcgttatt gcggctattg tgatcgggat tttcatttat gtactgacga
    30841 tgcaaaagat taatatcttt ggtgtaatga aagcacaagg gatcacagga ggatttattg
    30901 cgcgttctgt agtggcacaa acattcattt tatcatttgt tggtattctt cttggtctat
    30961 taggaacagt gggaacatcc cttgttctac ctgatgcggt tccattccaa agcaattggc
    31021 tgttttttgg cgtcattagc ttgttgatgt tagtagtagc tgtgttaggc gcactatttt
    31081 ctgttcggac aatcgtaaaa atcgatccgt tgaaggcaat tggataagaa ggaggaagaa
    31141 catgaaagca atagaattta aagaagtaga aaaaagtttc caagatggcg accaagtagt
    31201 taaagcattg aaagaaacaa ccatgagtat cgataaaggg gaatttgttg caatcatcgg
    31261 tccttctggt tcaggtaaaa gtactttttt gactttggca ggcggattgc agacgcctag
    31321 cagtggctct gtatttatca atgatcaaga attcagtaat caaaaagaaa gcgaacgcgt
    31381 taaactacgt tttaaagaaa ttggctttat tttgcaagct tccaatctgg ttccttttct
    31441 gacagtaaaa aagcagttat tattagtaga taagattaaa catgaacatc gtcaaaaaga
    31501 agcaaacgaa ttatttgagc agcttggcgt ggataagttg atcaacaagt atccagaaga
    31561 actatctggt ggagaacggc aaagggtcgc aatcgctcgt gcattgtaca atgatccgtc
    31621 cattattcta gcagatgaac caactgctag tttggattca gaaaaggcac gagaagtagt
    31681 cgatatcttg gcaaaagaat caaaagacaa aaataaagca acgatcatgg ttactcacga
    31741 tacacggctg attgacaaat gcgatagaat atttatgatg gaagatggtg tattgaaaga
    31801 aaaagaataa ccaaaaagca ggtggaaaaa tggtccatcg atacaacaaa gatacacagc
    31861 aaaaaatttt gaaagaagcg actgacttgt ttatgtccaa gggatatctc ggcacttcaa
    31921 caagagaaat cgcacaaaaa gttgggatca cccagcccaa tctctatcat tatttcggtg
    31981 ataaagaaaa actatatacg gcagttttag aaaaccactt aaaagatgtt ggaaaagctt
    32041 tgagagaaat cgttcaaaca agcgaaacgg gatttcaagt aaccttgaca aaaatggcgc
    32101 agtttttgat tgagacacat ctggtcgatt tgtttctgat gcttcacgat cttgaaagca
    32161 atctttcaaa ggaaacaaga gatcatcttt tctttctatg gaagaaaaat tatcgtgaac
    32221 cttttgaaga aatcttttca gcaaaccaat ctgtgctaag aaatgggatt tcaaaagaaa
    32281 tcgcagcaag gcattttttc ttagtgttag caccttacat cactcggtcg aaaagacctg
    32341 tggaagaacc gctaaatgtg gcgcaattga tcgatttata tttgaatggt gtattaggag
    32401 aagcgtaaag ctgttatata gggagaaaat agttaggtga agaagcaaga tatgacgaag
    32461 atcgttatat cttgcttctt tatataggta gtttttcata aagacaaatt tgttattctc
    32521 tcatgattat cagggtatat ttttatttat tatgtcggtg ttccaggaag tgttttaatt
    32581 taataacgaa gaaattaatt gtttgtagaa tgaaggaaac tatttattgg gtagatgaat
    32641 caccacgaat agtcaaaagg agatttcagg atatgccaaa aatgaaaaaa atcattttag
    32701 cagtaatgtt attttctacg gttatatctt ttacagcccc ttttttgctt acttaagctg
    32761 attttaagtt ttggccctta gtctggatct acgaggtgag agtcaagaat gaaaaaaata
    32821 attggagctt ttgtatttgc aatcattatc attattatct gttttcaaat agatgttttt
    32881 ggtcaaaaaa agatagttgg acaaacggtc agcagtacga acagtacggt tacaaagaca
    32941 aaggaatcat ttgatgatac agaacaagag acattaatca aaatcggaaa aaatgaccgt
    33001 acactatata cagatgactc gctaactacc ccacttgcaa ctataaacgg cggagagctg
    33061 gcggagtttc tttctgaaac aaaaaatgct tatcagatca agacaaatga tggctatact
    33121 ggatatttag ccctcgaaga tggaaccaaa gtcacaaaga atatacaaac aaaaccggaa
    33181 gagctatctg atgcagtgat tgtattagat cctgggcatg gcggaaatga tactggggca
    33241 ttaagcaatg atgaacaaac agaagaaaaa gatataacat taagtacagc cataaaagta
    33301 aaaaaggcgc tagaggatgc tggagcaacg gtttatttga cccatactac agatgaatta
    33361 gtacagcttg gagatatatg cgactacagt gaagaaaaaa aggcagatgt ctttatcagt
    33421 ttacacgcag actctacgga atatgcgaat gaagcaactg gaatcacgac atattattac
    33481 tatggacaag aggaaacatt agcacagaca attgctgact ccttcactga tctgcctctg
    33541 gattcaagag gaatctccac tgggaactat caagttcttc gagaaaatct tcaaccttct
    33601 attttaatcg agatgggtta tatgaataat gatagtgatt taaaggaatt agtaacagac
    33661 agttatcagc agcagattgc tgcaagtttg acacaagcat tgaccactta ttttcagtaa
    33721 taaataagcc cagccaaaca aaaatagctc ctcatatttt tcgtttggcc gggcttatta
    33781 tcgcagatgc aagcttttga acaccgttta tttttttagt agcttcaaag ctttttctgt
    33841 atttgcaaag tgaagtttgg catatttttc aagcataggc atgccgcctt tctccaatag
    33901 ttttgccgct acttggtcag atttcgttcc agctcctgat ggaagggtga ttccagcttc
    33961 agctgatgct ttgtgtaact cttccaaaaa gctggcacga ctgatgatag aagcagcagc
    34021 aacagctaaa tgatattgtt ctcctttagt aacgaagtac agtttttctt gtaattgatt
    34081 tttttctaaa cgcgcatatt ttttaaaatt tgcttccggc gtaaactgat cgatcaaaat
    34141 cgcttctggt ttttctggct cgattttttt tagcagcaaa tggatcgctt gattgtgcaa
    34201 agcgactttc atccgaacgg cattgtattt tggttggatc tcattgtatt tttctggaga
    34261 taagacaagc agtttatagg ggatggtttt ttttaagatg tcagcaagct ggatgatttg
    34321 gctatctttt aactcttttg aatctctaac accaagttta cgtaattctg caagctgatc
    34381 acgtttcaca taagcagcac atactgttag cggaccgaaa tagctgccat ttccgacctc
    34441 atcactacct aaaacggata attgactgat attttttggc aaagaagagg ctgtttttgc
    34501 tggcttggct tgtttatcct gtgcctcccc ccatttatca gcttctattg ctgcattcgt
    34561 accttgaaac atcacttttc cggatgtata agcggtaatg gtcgtcccgt ctttttttgc
    34621 agcaaagcta gtataaggaa tctgtttatt taacaaataa gagcgatagt atgctttcat
    34681 ttgactaagt tggtcgtttg ttgctttgat aatgacgttg ctcataaaat tctcctcgtt
    34741 tttatacata aaactatacc gcaactcacg acttttcgtt ttctaagaga ttgcgagaca
    34801 tagcattttt ttaaagttta tcatataata gttaagtatg tgaaaaatat ttgtggagaa
    34861 cggtgaaaga accaacttca tggtacacta aataaaactt tcatcaaata ggaggaaatg
    34921 attcgtatgg cgcacgaaaa aacacgctac aaagcggtga ttgccaacca aacatatact
    34981 attatcggac gggaaaccaa gcatcatatg gatatcgtaa cgaagctgat caatgaacag
    35041 ttagcagaat tgaaacaatt atcaccacaa atggataatg aacaagcagc aatcttgatg
    35101 gctgtcaatg cgctttcgga tcaattgaag aaacaagaaa gaatcttgga gctggaagaa
    35161 gaaacagcag aattaaagaa aaaaatgatc aaatttacag agcttgaaaa tcgtgtgaaa
    35221 cgcattgaag caatcgagaa cgaagctcgg gaagtattga aagaaaatgg tcaagcagat
    35281 cataccatta aaaatcatgt ggaagcacaa cagattttaa acgagaaacg aaaaggacaa
    35341 attaaacaaa aatcttctaa ttaagatcct tttatcaaaa taagaaaaat ttaaaaaaag
    35401 ttagaagaga aaggaacgta gcatgctgag tttgctgatt ttatttttat tgctaattgc
    35461 gtttttttct ggagcacgtc gcgggtttgc tttgcaaatc atattcgcga ttggatatgt
    35521 attgtcattt attgctgccc agcattttta caaaccatta gcaagtcatt tggaattata
    35581 cattccatac ccagctgtca cgccaacatc aaagttagca ttttttgatc aggtatttgc
    35641 ctttcaccta gatgaagctt tttatgcagg aactgctttt ctgattattt tgctaattgg
    35701 ctggctgctt acccgatttg tgggggtttt tgtccatgga ttgacgtatg tgccgatctt
    35761 gagacaggta gactggattg caggaggcat cctgtcacta atcatggctt acgttactat
    35821 atttttgatt ttgcagttac ttgcctttgt tccattagat gtggtccaaa atcaattcgg
    35881 cgagcatagt ttcgctcgct ttatcgttga gagaacgcca tttcttacca acaagatgta
    35941 tgagctttgg gtaacgaata ttttgaatta aggaaaatag tgctggaaaa tatttcgttt
    36001 tttgtacaga gtagattatt taagaaggct gcgtagcgta tgtccggcac agctcttttt
    36061 tcatagtgaa agataaggtg ataaaatgaa taaccgaatc cttgaaacat tggaatttga
    36121 aaaagtcaaa cagatggtca gacaatttgt agtgactgca caaggaaaag aagaactggc
    36181 agaattggta ccagtgagcg aggaacaaac gatcacaaac tggcttcaag aaactgagga
    36241 tggattgaaa gtgcaacgtc tgcgaggcgg gatccctata cctaaacttg aaaatatccg
    36301 tccccatatg aaacggattg agatcggagc agacttgaat ggagtggagc ttgcacaagt
    36361 ttccagagta ttgtctacga caagcgaatt gaatcgtttc attgatgatc tttatgacag
    36421 tgaaatcgaa tttgcacgtt tgtatatgtg ggcagatcag ttagtcacga tccctgtttt
    36481 gagccgccgt ttaaaggaag caatcgatga agacggacgc gtgactgatg acgcttctcc
    36541 tgagttaaag agcataagac agaacattcg gcgaagtgaa caagctgttc gtgaacagtt
    36601 agatgggatc gtccgtggga aaaatgcaaa gtatttaagt gatgcgatca tcaccatgcg
    36661 aaatgaccgc tacgttatcc cagtcaaaca agaataccgc ggtgtatttg gcggtgtagt
    36721 ccacgatcaa agtgcttcag gacagacttt atttattgaa ccaaaacaag tggtggattt
    36781 gaataatcgt ttgcgtcaat accaaatcgc tgaacgaaat gagattcaac gtattttaag
    36841 tgaactttct gctgaacttg ttccccatcg tcaagaaatc atccacaatg cttatgtgat
    36901 tggtaaaatg gatctgatga atgcaaaagc acgatttggt aaagaagtaa aagctatcgt
    36961 gccgggaatc agtgaagaca atcatgtcgt cttgaaacaa gcaagacatc cactgatcga
    37021 tcaggaaaaa gtagtttcta atgacatcac aatcggaaaa gactatcaag ccatcgtcat
    37081 tacaggacct aacacaggtg ggaaaacgat cacgttgaag actttaggtc tgttacaact
    37141 gatggggcaa gcaggattac cgattcctgc aggtgaagaa agtcaaatcg gtatttttga
    37201 ggaagtattt gcggatatag gagacgaaca gtcgattgag caaagtctat cgaccttttc
    37261 ttctcatatg acgaacactg tagatatttt atcaaaagtc aatgaaaaaa gtctggtatt
    37321 atttgacgag ctcggtgcag gaacagatcc gcaagaaggt gctgctctag caatcgcaat
    37381 cttagatgat cttggtaaaa agtctgctta tgtgatggca acaacgcatt atcctgaatt
    37441 gaaagtttat ggatataatc gggcaaatac gattaatgcc agcatggagt ttgatgtgga
    37501 tacattaagt ccaacgtatc gtttgctgat tggcgttcct ggacgaagca atgcgtttga
    37561 aatctctagc cgattaggtc ttgatacaga agttattgat gaagcgaagc agttgatgaa
    37621 tgatgagagc caagatttaa acgaaatgat cacagattta gagaatcgcc ggaaaatggc
    37681 agagaccgaa tatctggaaa tgcgtcattt tgtttctgag gcacaagaac ttcacgatga
    37741 tttgaaagaa gcttacagct atttctttga agaacgtgaa aaagaaatgg aaaaagcaaa
    37801 gaaaaaagca aatgaagtcg tgtcagaggc ggaagaaaaa gcagaaaaaa tcattgctga
    37861 tattcgtaaa atgcaacagc agattgggca aggaaatgtg aaagagcatc agttgatcga
    37921 tgcaaagacc caattagcaa atctacatca agaagaaact ttgaagaaaa ataaagtatt
    37981 gaagaaggcg aaagaacaaa aaacgttgaa accgggagac gaagtgttgg ttacgactta
    38041 cggccaaaga gggacgcttt tacgtaaaaa cgggaatcag tggcaagtag aaatcggtat
    38101 attaaaaatg aatgtgtccg aagacgagct gacgcctgtt gctcctcaaa aagagccgac
    38161 acaacgagtt atccatgctg tacgatctga atctagtagt cacgtgccga atcaattgga
    38221 ccttcgagga aaacgatacg aagaagcgct aagtgaggta gatcaatatt tggattctgc
    38281 cattctagct ggttatcctc aagtgactat tgtccacgga aaaggaacag gcgcacttcg
    38341 caagggaatt actgattatc ttaaaaacca tcggagcgtc aaaagctttg agtttgcacc
    38401 ggcaaaccaa ggcggaaatg gtgcgacaat tgttaaattt aagtaaagct tactaaaggt
    38461 aagcagatgg ttcgctatcc aagtatagaa atgctatact agtcatagat tttaaaaatg
    38521 gaggtctttg atatgtcaca agtaattaca gataagacat ttaatgaaga aacagataaa
    38581 ggacttgtat tgattgattt ttgggcaact tggtgtggtc catgtcgtat gcaagcaccg
    38641 attttggatc agttggaaca agaatatgat gaagaagaat tcagaattgc taaaatggat
    38701 gtggatgaaa atccagaaac tcctcaacaa ttcggtatca tgagcattcc aacattaatg
    38761 ttgaaaaaag atggtcaagt agtagaaaaa gctgttggtg ttcattcaaa agaacaacta
    38821 cgtcaaatga tcgatcaata tttataaaaa cttacagcaa aaataagctg agggtgggac
    38881 tgaatccagt tccgtcctcg gtttttttgc ggttgagtta aggttattca aattaatatt
    38941 ttttgagtat aattacgttg tcaggctgga ttgttttttt gacagacatg ctataataac
    39001 tttaattgaa tatgaagggg agtaccagat gaacgaacga atcaaaaaca aattagcttt
    39061 acttcctgat cagcccggct gttacttgat gaaagataaa aatggaacga tcatttatgt
    39121 cggcaaagcc aaaatattaa aaaatcgtgt ccgctcatat tttactggta gccataatac
    39181 gaaaacggaa cggcttgtta gtgaaatcga agactttgaa tacatcgtta cagaatcgaa
    39241 tatcgaagca ttgctcttag agatcaatct gataaagaaa aatgatccta aatacaacat
    39301 tatgttaaag gatgataaaa cctatccttt ccttaagata acgaatgaaa aatatccccg
    39361 tttggtgatt actcgtaagg tactaaaaga caaagcactg tattttggac catatcccga
    39421 tgtcggtgcg gcgaatgaga caaagaagat cctcgatcga ttatttcctc tgagaaaatg
    39481 caaacctagt cagacgaagg agccttgtct ttactatcat ttgggacaat gtctgtgtcc
    39541 ttattatttc gacgtagatc cgaaagtcta tacgaaaatc gttgatgaag tgaaaaactt
    39601 tttaaacgga ggacacgaaa aaatcgagtc agagatccat cagaaaatgg aaaaagctgc
    39661 tgaaaacatg gagtttgaaa aagctgcaga ataccgagac cagatccgtg ccattgagac
    39721 aatcatgact cgccaaaaaa tgacaaatac tgatttactg gatcgagatg tatttggtta
    39781 tgcgattgat aaaggatgga tgtgtgtcca agtgtttttc gtaagacaag gaaagctaat
    39841 tgaacgagat gtttcaatgt ttcctttcta taacgaagca gaagaagatt tcttgactta
    39901 cctcggtcag ttttatcagg aaaatgagca tttcattcct aaagaagtct tgattcctga
    39961 tacgatcgac aagcccagtg tagaagcttt gttagctacg aaagtgctgc aaccaaaacg
    40021 aggagaaaag aaaaaattag ttgaattggc gagtaagaat gcctcagttg ctttgaatga
    40081 acggtttgat ctgattgcca gaaaacagga acggacgatc ggagcagttg aacgtttagg
    40141 aaacgcgatg aatatcccga ccccaatacg tatcgagtct tttgataact ctaatatcat
    40201 ggggacgaat cctgtggctg cgatggtcgt attcattgat ggaaaaccgg caagaaaaga
    40261 atatcggaaa tataaaataa aaacagtcaa cggaccggat gattatgcat caatgagaga
    40321 agtcatctat cgcagatatt cgcgagtgat tcgcgaaggg ttgcctttac ctgatttgat
    40381 cttgatcgat ggaggaaaag gacaagtaga tgtggcaaaa gatgtcttag aaaatcaatt
    40441 aggaatcgat attcctattg ctggcatggc aaaaaacgat aaacataaaa caaatgagtt
    40501 gctattcggt acagatttgg ctgtagtacc gcttgagcga aattcaccag agtttttctt
    40561 actccagcga atccaagatg aagtccatcg attcgcaatc actttccatc ggcagctacg
    40621 cagtaaaaac agttttgctt cccgattaga tgaaattgaa gggttaggac caaaacgaaa
    40681 aaaaatgctg cttaaagagt tcaaatcgtt gaaaaatatc acagcagcca cgacagaaga
    40741 attacaagaa atcggactgc cgaaaaatgt cgcacaaaac gtatttgata aattacatca
    40801 ggaatgagga aagggcactt tacaaagaaa gtaaagtgtc ctatcatttt atgtacaaca
    40861 aaaagagcgt ttacgttttg ttggtaaata ataaatcgct tacgtctggt gtctaaggat
    40921 atatgtgatt tcttgaagaa ggagtgaaga aatgttagaa gtcaaggatc ttgttaaaac
    40981 ttttgggaac tacacagctg tcgatcatgt ctctttccaa atccccgatg gaaaaatcat
    41041 ggggctaatt ggccaaaacg gggctgggaa gacaacgact tttcgtttga tcttggattt
    41101 tttgacacaa gaccaaggag aagtgttatg gaatggccat catcttgggg agaaagatta
    41161 tgatgtcatc ggttatcttc cggaggaacg gggactttat ccgaaagtaa cgattcaaga
    41221 ccaactgctt tattttgcag aacttagagg gaaatcaaga aaagagattg aaccaaaaat
    41281 cgacttttgg atggaaaaat ttcaggtaaa agggaaaaag acggataaag taaaatcttt
    41341 gtctaaagga aatcagcaaa aagtccagct gatcgccacg ttaatccatg aacctaaatt
    41401 gatcattttg gatgagccat tcagcgggtt agatccggta aatgctgaat tgctaaaaga
    41461 tggaatattg gaattgaaaa aacatggttc atgtgtcatt ttttctagcc acaatatgga
    41521 taatgtagaa aaaatctgcg atcatttgat catgttgcga aacggaaaaa tggttttgaa
    41581 tggaaaagta catgagattc gtgaatcatt tggccgtacg aagttattct tggaatctgg
    41641 tttaagccaa caggaagtag aagaaatcga aggcgtgaaa aaagtgatcc ttcatgaaga
    41701 caatacgtta gagattacct tagaagatcc tgaagctggg aaagaaatct ttactcgtgc
    41761 gactcagttc ggctacattc ctatgtttaa ccagcaaccg ccaactttgg aagagatttt
    41821 caaattgaaa gcaggtgaaa tcgatgcgta agttttggat tattaccaaa gatgtctatt
    41881 taaaaaatgt taagtcgatt tctttcttga tcatgatatt agtccctttt atcatgctgg
    41941 gtatcattta tgtagcagga aactttgctc aacaaaatag tgatgtcaat aaaatcggcg
    42001 tattggctga taatgagcaa atcagtcagc aaatgagcca agtcaaaaca gatgattttg
    42061 agttcaaagc agttgattct gaagaaaaag cacaaaaaga gctttcggaa gaaaaaatcg
    42121 atgcgtattt agtagtctca actgagaatg ataagataaa tgggatgctt tatagcgaaa
    42181 attcattagg acaaagtaca caactactga tacaacaaca actgactggc ttgcaatcga
    42241 tgcttcgagc gagtcaatta ggcatttcac cagaagaagt agctagcttg agccaacaag
    42301 caagtttgac tcgacaaaaa gtcaattttg attcaaaagg aaaaatgaca gtcggagaag
    42361 ataactcgac agtccaatac gtcgtaagtt atgtagcaac gattgtcttg ttcattatta
    42421 ttttgacgta tgcgcagatc attgcacaag agatcgcttc tgaaaaaggc acgcggatca
    42481 tggaagtgat cctgtccagt acgactgcac aaaagcattt ttacggaaaa ttaacaggtg
    42541 ttttacttgt tgcattaacg caaatggcat tatacggtat tatttttggt ataggataca
    42601 gtcaattcaa agatatggaa atggtcaaaa atatattaga cggtatctca ttggataact
    42661 tgattggacc attcttgtgg tattctcttc tattcatgat ctttggcatt ttgatttttt
    42721 ctgttttagc agctttatgt ggctctttgg tcaacaaagc agaagataca gcaaaagcaa
    42781 ttttgccagt gacttatcta tctctaggcg gatacatgct tggattgata ctaggagctt
    42841 cagatccaaa caatatcgtt attcggatca cttcgtatat tccattctta tcttcatata
    42901 ttatgccggt tcgcttagca aatgagacag tcggtatggg tggagcattg atttccttgt
    42961 tggtacttgt agccgtaaca ttcttattga tgatcttgtc tgcaaacatg tacaaatcga
    43021 atgtactcgt ttatagcgaa ggcggattat ggagttcatt gaaacaatcg atttcaatta
    43081 tgaggaatga acgaaaaaaa gcataaaaca atcctaaaat acagaaggaa gctggacatc
    43141 tattgttccc agcttctttt tgtatagacc tattttattt attttactat attggatata
    43201 tattcattga aaatagtgta taacaacttt ataatattga gtataccaat ttttggaggc
    43261 gttttaatgg accgttattt agatttactt tcagaaaaat tcccctcaac ggaggatgtc
    43321 atcacggaaa tcatcaatct cgaagcaatt ttgcagcttc caaaaggaac agagttattt
    43381 ttgagtgata tacatggcga atttcctgct tttgatcata ttctgcgtat cggttcagga
    43441 aatcttaaag aaaagatcaa agaccttttt tctgaacaaa tgacggaaac ggatatcagc
    43501 caattcacta tttttacggc ttatccagaa tatgcattaa cgactgattg gtacaaagaa
    43561 caagataaat cgcagctgat ccatcatttg atcgatcttt tacggtttac gacagtcaaa
    43621 tatacccggt caaaagttcg aaaaggattg ccgaaagaat atagctatat cattgaggaa
    43681 ctcttgtata tcgatgaccg tatcaatgga aaaaaagact atgtaaaaaa aatcattgaa
    43741 caacttgttt tgatgaagga agaagaaaaa tttttaacga aacttgcaca gacgatccaa
    43801 aaatctgtca tcgatcattt gcatatcgtt ggagatattt ttgaccgagg aactcaagca
    43861 gccaaagtga tggatcgaat catggatttt tcttctgtag atatccaatg gggaaatcat
    43921 gatattttgt ggattggtgc gtattgcggc tctgaggcat gtttactgac acttttacgg
    43981 attgcagcac gctataatta tttatttgaa ttagagaaag aatatgggtt gaatttacgt
    44041 ccattgtttt cttttgctca tcaaacttat cagaagaatc cagtatttgc gcctaaaaac
    44101 agtaaaaatc tatccgaaag agagcaagaa gaattagaaa aaatccatca ggcactttct
    44161 atccttcaat tcaaatcaga acatcagtta ttggatcgtc gacctgaatt taaaatggac
    44221 gatcgtaaat tgctggaaaa aatcgactat gaagcatcaa cgattgattt agaaggtcag
    44281 ctctatgagt tgaaaggaac ttgttttcaa acgatccagc cagatgatcc aaaaaaattc
    44341 ttggaagagg aacaaaaagt gattgatagt ttgatgtatt cattccagca ctctttgaga
    44401 atgcaaaaac atatgacgtt tttgatcgaa aaagggggaa tgtatctcac gaacaatcag
    44461 catgtgctgt ttcatggctg tatacctgta gacgagaagg gacagctctt agcatttgaa
    44521 ttagaccaat cttatcgcgg aaaagaactg ctgggctttt ttgaaaaaca gataactgaa
    44581 agcgcaaaag atctgactgc aaaagaagat ttagcaaccg atttgatttg gtatgcgtgg
    44641 agtgggccat tttcgccttt attcggcaag agtaaaatgg cgacttttga acgttatttt
    44701 ctcacagagc ctcatacgca cgtcgagaaa agcaatcctt attatcactt gcgagatgaa
    44761 gagtggttca gtgaaaaaat tttagcagaa ttcgacacaa aagaagaagg gtcagcaatc
    44821 attaatgggc acacgccagt aaaagtaaaa aaaggggagt caccgatcaa agcaaatgga
    44881 aaagtatttg tcattgacgg aggtttatcc aaagcttatc aaaaaacgac aggaattgcc
    44941 ggttattcac tattatgcaa ttcttatggt ttccaaatcg tgacacatta tccgtttttg
    45001 cctattgaac aacaatttgc aaaaaaatcc gaccaaacaa atgtgaaaaa agtaatcgaa
    45061 cagcaactac cacgcaaact caatcgtgat acaacaaaag gtatggaact gcagcagcag
    45121 attacccagc tcaaaaggct gttggactat cgaaaggacg attgagaaga attccagtga
    45181 acattgttcc atagactgga gactgagaca aaagtcgtgt cgcagtctct ttttccggat
    45241 aaacggtgtt cttctgccaa ctcctcggtc acaagtcaca ctattcaaca aacatgaaaa
    45301 gctgtttttt gaaattgctc cttgtgactc agagttgaac ggcaggctca cgacctcttg
    45361 gtataaacgg tgttcaaaag ccagtttctc ggtattaagc caaatttatg caaaaatgtg
    45421 aaaagccatt ttcacaaaat ttttcttaat actcgaaact aaacagcttt ttcacaaccc
    45481 cctcagaaag tctattaaaa ttcattcaca aagaaatcga ataagcgctg ttcagatgga
    45541 tgtacacggt gggtaagttc cggatgccat tgaacaccga ggattttatg catatcatct
    45601 ttgctttgaa ccgcttcaat gagtccatcg ttagaaaaag cgatggcttt taataaaggt
    45661 gaaagatctt tgactgcctg atgatggtaa gaattcacaa aatatggatc ggggagaagg
    45721 ttgcttaaca aactgtctga aacaatcttg acttcgtgag tcgtaaattg cggggcagtg
    45781 ggctgttgtt catgttttac tgaccacttt ggatataatg aaagatcttg ataaagtgta
    45841 ccttccaaaa cgacattcaa tagttggagt ccccggcaga ctgcaaaaat tggttttctt
    45901 tgtttcaatg cttccaagat cagtgcctgt tcaaattggt ctcgttgaat atttgtttcc
    45961 gttagctttg gatgaggatc ttccatgtaa agttgtggac tgacatcttg accacctgca
    46021 agaataagtt tgtcgatttg tccaatcagt tgaggagctg aatctggaga catgactggc
    46081 agaataagag gcaatccttg agcatctaaa actgcatcca cgaatccttg cggtgtatag
    46141 ctcacttgat ttccttggaa agtatctgtt gctcgaatca gttgatttcc ggcaatgccg
    46201 atgattggtt tcatttgtat tccccctaaa gtagacttat tgtaacaagg atttaaaaaa
    46261 aattaaacta tgtaagactt gcccctatac ttatctgttc ttgctgtgtt actatctgta
    46321 aagagaaatt tcgtgtgaaa ttcatgacga tagttgaaaa tattgcttaa tttgttataa
    46381 taaaaagtaa gcagaaataa aaaggttggg cgtaagcttc aaggatctag ttcttcaagg
    46441 ggtgagaagg acattttatt gaaggggcct gcctatacta agttatcttt agaggtacat
    46501 actttcaatg aaaaaagaaa gcatgtacct cttttttaaa tcgaaaggaa gtgaagcaag
    46561 tgttagaagc tttggaacaa atccgaaaag cagaagaaga aaatgaaaag cgtgctcaaa
    46621 cactgcggca gcagctgaag atctatgcaa cagaaaaaga gcaggaattg gaaacgatcc
    46681 aaacaaacta tcgacaacaa ctaaaacagt tggtggaaga aaaagaacaa tttgaaatac
    46741 aagaattaga aaaagaaaaa atgaaattga aagcagaagc agacaaggtg caagcaactt
    46801 tgaaagatca atatcaaaag catcagagtc aagcgatcgg tgcgatcatt gaaagggtga
    46861 aagaaactta tggccgtcac taagatggaa aaagtgaccc tcatttcaga taaaagaaat
    46921 caggagagga ttttacaagc agtacaagga atccaaaacg ttgaaatccg ggatctgtat
    46981 caaaatagaa caaacaacca gtgggtggag aagtattttc cagataccac gattattgat
    47041 aaagaagcaa aattaagcgc tttaggtaat cggttgaaag acatccaaga agctatccaa
    47101 ttcatacaac accacggcga tagccgtcaa aagaaactcc atttgaaacg tcaagaactt
    47161 tccttacaag atttggaagc caattattcc gaagaaaatt tcataaataa attgaaagaa
    47221 gtccttgatt taaaagaaaa gtgggaacaa ctaactgaag aacgtgatcg tttagaagaa
    47281 gaggaagaat ggcttcttca ttggcaacag ctggatattg ctccaagtat gtatgcaagt
    47341 aagacgacca tttttgagat ggggactgtg agtgcagcaa attttgaaag tttcaaggaa
    47401 gaactgagta agcttgatga cgtttatatg gaagaaatag attatacgcc aaaacaagtt
    47461 tatctttcgt atgttgcttt aaaaaaatca gttgctgtta tagaagaaat tgctgggaaa
    47521 tacgggttca ataaagaaac gtatccttat gaacaaaatc ctaaagaaaa actgcaagag
    47581 accaagcagc aattatccat tgttcacgag cagcaaaaga agataacgac agcgttaggt
    47641 cagtgcagtg gctatatcca agaatttgaa tgggtagaag aagtcacact tgccgttgca
    47701 gaacgagaaa aaataaaaga acggtttatc catgcgactt atctgatcgt tttgcaaggt
    47761 tgggtagatg cagaagaaaa acagaatttg ctgcatgtgt taaacgaaac cgtatcagaa
    47821 gaagatatat acgtcagttt cgaaacacca aactcagaag agattcagca ggaagtccct
    47881 actaaattga aaaaccatcc gcttgtcgaa ccttttgaat tgctgactga aatgtacagt
    47941 ctgccaaaat atgaagaagt agatccaaca ccttggatga caccatttta tcttgtcttc
    48001 ttcgggatga tggtcgcaga tgttggttat ggtttgctga tgctcattgg gacaatacta
    48061 gctcaaaaac tacttgttct accacgcggg atgaaacgtt tcgtaaaatt ctttgagatt
    48121 ctctcgatac catcgatcat ctggggattg atctacagtt catttttcgg acagtcatta
    48181 ccaaaagaac tgtttggcat ccgcttgcct ttcccaatcc tatctacgac ggatgacgtc
    48241 aatacgattt tgattttatc cgtgattttc ggtctgatac agattttggt tggattattt
    48301 gtggcagcaa aggaaaatat caggagaaaa gattacttga gtgctgtcag tgatggattt
    48361 gcctggcaag ggatcctaat tgggatcgtt attgcgttgg ttggtgccat gttgataaaa
    48421 aatacagcat ttgtttacct tggcgctggg ctatcgatca ttgctgcctt atgtatcttg
    48481 atcgttccga ttatccaaac gccgtctaaa gtcaaaggtg ctgccaaagg tgcgtataat
    48541 ctttacggac tgaccggcta tatcggtgat ttagtcagtt atacacgttt gatggcattg
    48601 ggtatttcag gcggaagtat tggagcggca ttcaatatgc tagttgcttt catgccgcca
    48661 gctgctcgat tcagtgtagg gatccttttg atcatcgctt tacacgcact gaatatgttt
    48721 ttgacactgc taagtgccta tgtccatggc gcacgattgc agtatgtcga attttttggg
    48781 aagttttata caggaggcgg acgtgccttt gatccgctga aaacggcgga aaaatacgtc
    48841 aacatcaatc acaagaaaaa aaacaataaa taattggagg atttttatat aatgatggat
    48901 tacttaatta gtcaaaatgg tggaatggta tttgcggtat tagcgatggc aacagcgacg
    48961 attttttcag ggatcggttc agctaaaggt gttggaatga caggtgaggc agcagcagca
    49021 ttgacaacaa gtcaacccga aaaattcggg caggctctga ttctgcaatt gcttccaggt
    49081 acgcaaggtt tgtatggatt cgttattgcc tttttgattt ttatcaactt aggaaatgac
    49141 atgtcagttg tacaaggact gaactttcta ggtgcttctc tgccgattgc ttttacaggc
    49201 ttgttttcag gtattgccca aggaaaagtc gcagcggcag gtattcaaat tttagcgaaa
    49261 aaacctgaac atgctacaaa aggaatcatt tttgctgcga tggttgaaac atacgcgatt
    49321 ttaggtttcg ttatttcctt cctattagtg ttgaatgcct aagcataaag gaggaaaagc
    49381 agatgaacgc cattgaaaaa atcatctctc agatgaacga agcagcagag caggaaagag
    49441 cggcgttaga acaagaagag cgaatgaaga tcgatcaaaa ctttgaacaa aaacgtacgc
    49501 aagttgaaac agaacatcaa aaacaaaaag aaaaacaaat cgaattactc gaaaaaaaat
    49561 accgtcagct aaggaatcgc cagcaagtag aagtacgaca agaaaatttg aatgcgaagc
    49621 aggaattttt aagacgattg tttgcagatg cggtaactga aatggaaaat tgggatgaat
    49681 ctgaacaaat ccagtttatc aaaaatgcat tgtattcatt acctttgaca ggaaaagtag
    49741 cattcattgc tggagaaaaa tcagcagcct atctttctca gacgttgttg gatgaatgga
    49801 acaatgaact tccctttatg atggttttaa gtgatgaaac tgttgctgat caagcgggtt
    49861 ttctgataaa cgatcaagga gttcagtata attttctttt cagcagcctt gtacaagata
    49921 tccaaggaac gatgagtttt gagattgcca atcaattatt cgaataagga gggatcagtg
    49981 tatggaatac cacgaactaa atcccttgat tcgcggaaga gagcttgaac ttttgtccaa
    50041 tgagacattt gaacggatga ttcaaacaaa ttctgtcgaa gcacttggtg aaatattgaa
    50101 atccacgatt tacagtccat atatttatga cggatatgaa tatgacttcg aacagaatct
    50161 tttaaaggag cagagccgtc tatttcgctg gttaaaggaa tcagcaccgg agcctgaaat
    50221 cgtttggatc tatacgatgc gttatacttt ccataatctg aaggttttga caaaagctga
    50281 gatgacaaat caaaatctgg accatttgta tatcgacgat ggattttatt cattagaaac
    50341 cttgaaggat gccattcata cgcaagtttc aaatgaactt cctgaaagca ttatggacag
    50401 tatacgagaa gtacgtgatt attttgaaga gtctaccatt cttcagggaa ttgacgtgat
    50461 ttatgatcga cagtttttga cagagcagcg acggcttgcc gataagctag gatatcaaga
    50521 attgctgaca gaagtcattg cttttattga cttgaccaat atcacaacga tggctcgagg
    50581 aatattgcag aaaagaacat atgggttcat gacgacggta ctttccagct cgggaagtat
    50641 cgaaaaagaa gtatttttga actttgtaga taaagatatg gaagattaca cgaattttct
    50701 attagagaca gattatgctg attttctgcg ccctgtcatc cgtgagggaa atattgatct
    50761 cgtaaagctt gaacagttga aagatgatta tctaagttca ttgtatcaag aagcacaaac
    50821 ccaggctttc ggaccactgc cactactagc ctttttgaac gcaaaagagg tagaaagcaa
    50881 aaaccttcgt ttactggtta tcggcaaaag aagccatttc gaatcagaaa caatcagaaa
    50941 gcgggtgaga caagtctatg acgcataaga tcggtgtagt tggcgacaaa gattctgttt
    51001 tgccattcaa attgtttggc tttgatgttc gttatggtac aaccaagcaa accgttcgtc
    51061 aggcaattga agaaatggca aagaatgagt atggcgtgat ttatgtcaca gagcaatgtg
    51121 cagcattgag cccagaaacg atcgaacgtt acaaggaaca aatgatacct gctatcgtat
    51181 tgattcccaa tcatcaagga tcattaggga tagggctgga agaaattcaa aaaaacgtag
    51241 aaaaagcagt aggtcaaaat atcttatagt atgaaaagga gatagaaatt ttgcaaattg
    51301 gaaaaatcat aaaagtctcc ggacctcttg taatggctga gaacatgtct gatgccagta
    51361 tccaagacat gtgtctagtg ggagatctag gagttatcgg agaaattatc gagatgcgtg
    51421 gcgatgtggc gtctattcaa gtatatgaag agacttctgg aattggtcca ggcgaaccgg
    51481 ttcgctctac tggtgaagct ttatctgttg aattaggacc agggatcatt tcacaaatgt
    51541 ttgacggaat ccaacggcca ttagatacct ttatggaaat cacacaaagt aatttcttag
    51601 ggcgcggagt tcaattgccc gcattggacc atgagaaaaa atggtggttc gaaccaacag
    51661 tagaagccgg agaaacggtg agtgctggag atgttatcgg gatcgtagaa gaaacaaaag
    51721 ttataaaaca taaaatcatg gtgccaaatg ggataaaagg aacgatcaaa acaatcgaaa
    51781 aaggttcatt cactattgat gaaacagtat gtgtcattga aaccgaaaat ggtgacaaaa
    51841 atttgacgat gcttcaaaaa tggccagtaa gacgtagtcg gccaatcaaa gaaaaattaa
    51901 acccggatgc acccatgatc actggacagc gagtgattga tacattcttc ccagtgacaa
    51961 aaggaggagc agcagctgta cctggtcctt ttggtgcagg gaaaacagtc gttcagcatc
    52021 aaattgcaaa atgggccgat gtggacatgg ttgtctatgt gggatgtggc gagcgtggaa
    52081 atgagatgac ggatgtttta aatgaattcc ctgaattgat tgaccctaat acaggagaat
    52141 cgttgatgga acgtacggta ttgattgcta atacatcaaa catgccagta gcagctcgag
    52201 aagcttctat ctatacggga atcacaattg cggaatattt ccgtgacatg ggttatgacg
    52261 ttgctatcat ggcagactcc acttcacgat gggcagaagc acttcgtgag atgagcggac
    52321 gtttggaaga aatgcctggt gatgaaggtt atccagctta tcttggttca cggcttgcgg
    52381 aatactatga acgatctgga agagtcattg cattaggatc agaaggtcgc gaaggaagta
    52441 taaccgcaat cagtgctgta tccccgtctg gaggggatat ttcggaacca gtcacacaaa
    52501 atacattgcg ggtcgtaaaa gtattctggg gtcttgattc cagtttagcc caaaaaagac
    52561 attttccttc gatcgattgg atacaaagct attctcttta ttcaacagaa gtagggaagt
    52621 atatggatca tatccttcaa gctgactggg catccatggt caccgagggc atgcgtattt
    52681 tgcaggaaga acagcagctg aatgaaatcg tgcgtttggt agggattgat tccctttcag
    52741 ataatgaccg tctgacatta gaagtggcaa aatccattcg tgaagattat ttgcagcaaa
    52801 atgcttttga tgatgtcgat acattcactt caagagagaa acaattcaaa atgttgaaga
    52861 tgatcttgac ctttggtgaa gaaggacgcc gtgcgttgtc gttaggtgct tatttaagtg
    52921 agatcatgga cggtaccgta tcgattcgtg aaaggatcgg acgaagcaaa tatatcccgg
    52981 aaacagaatt agaaaaacta gatcaaatta ttggtgaaat caagcaaacg atgcaaacga
    53041 tcgtttcaga aggaggcatg agcaatgatt aaagaatatc gtacgatcgg tgaagtcgtc
    53101 ggtccattga tggcggttga aaaagttgct ggtgtcaaat atgaagaatt aatcgaagtc
    53161 cgtatgcaaa atggcgagat tcgtcgtgga caagtattgg aagtccaaga agataaagca
    53221 atggtccaaa tattcgaagg aacaagtggt atcaatttac gtgactcttc tgtacgattt
    53281 ttaggccatc ctttagaact aggtgtatcg gaagacatga tcggtcgagt attcgatggt
    53341 ttgggacgac cgaaagataa cggaccagag atcctgcctg aaaagcattt ggatattaac
    53401 ggagaagtaa tcaatccagt agctcgggat tatccagatg agtttatcca aacaggtatc
    53461 tctgcaatcg atcatttgaa tacattagtt cgtgggcaaa aattaccagt attttctggc
    53521 tctggtcttc ctcataaaga attagctgct cagatcgcgc gacaagcaac ggtactcgat
    53581 tcatcagatg atttcgccgt agtctttgct gctattggaa tcacttttga agaagctgaa
    53641 tttttcatgg aagattttcg tcaaacgggc gcaatcgacc gttctgtcat gtttatgaat
    53701 ttagcaaacg accctgcaat cgaacgtatc gctactcctc gtatggcttt gactgctgca
    53761 gaatatcttg cttatgaaaa gggaatgcat gttttggtta tcatgacaga catgaccaac
    53821 tatgcagagg cattgcgaga gatttctgca gcacggcgag aagttccagg aagaagagga
    53881 tatcctgggt atctttacac gaacttagct actttattcg aacgagcagg aagaatccgc
    53941 ggactaaaag gatcggttac tcaaattccg attctaacaa tgccagaaga cgataaaact
    54001 catccaatcc ctgacttgac tggttatatt acagaaggac agatcatctt gactagagag
    54061 ctttacaaga gtgggattca gccaccaatt gatgtactcc cgtctctttc gcggttaaaa
    54121 gataagggaa ctggacccgg aaagacaaga gaagaccatg cagctacgat gaatcagctt
    54181 tttgcagcct atgcacaagg aaaacaggca aaagaattag ccgtagtttt aggtgaatct
    54241 gctttatctg atgtagacaa gatctatgcg aagtttgctg agcggtttga aaaagaatat
    54301 gtcaatcaag gattctacac gaaccgttcg attactgaaa cacttgatct agggtgggaa
    54361 ctgctatcga tgttgccaag aaccgaatta aaacgaatca aagacgacat gctggacaaa
    54421 tatctgccag aagggaagtg agttctgatg gcacgattga atgtgaatcc gacccgcatg
    54481 gaattgactc gattgaaaaa acagttgaca actgccacaa gagggcataa attattaaaa
    54541 gataagcaag atgaactgat gcgtcaattc atcctgttga ttcgaaagaa caaccagctg
    54601 cgtcaagaaa tggaagagtc tatgacagaa gcaatgtctg attttgtatt ggcaaatgct
    54661 tcgatcacgg aacctttcat cgaagaatta tttgttttgc cggcggaaaa cgttgatttg
    54721 tctatcgtag acaaaaatat catgagtgta aaagtacctg tgatgaattt tgaatatgat
    54781 gaacagctga cagacacacc tttggaatac gggtatttga attccagtgc cgaactggat
    54841 cggtcaatcg atcggtttac taccctgctt ccaaaacttt tggaactgac agaaatcgaa
    54901 aaaacttgtc aactgatggc gagtgaaatc gaaaagacac gtcggcgagt caatgcatta
    54961 gaatatatga cgatccctca attagaagaa acgatctatt atattcgtat gaagctggaa
    55021 gaaaacgagc gcgcagaagt aacaaggttg atcaaagtaa aaaatatggg taccactgac
    55081 taagtggtat ctttttttaa aagaggaaaa cgatgattaa acaaagcaaa caaataaaga
    55141 aacgtttgcc ggctgttcag cttctcgcat taggattttt tctcctgatt ttagttggcg
    55201 gcggcctact taccctacca ttttttagta atagtgggca tgggacaaat tttatcgatg
    55261 cgcttttcac tgcaacttct gctgtctgtg taacgggatt gacaacattg aatacagcag
    55321 agcattggaa cagtgctggt caatttttga tcatgctttt gattgagatc ggcggtcttg
    55381 gttttatgat ggttccgatt ctatgctttg cattgataaa gaagaaagtc agtctcagca
    55441 cacgtattat attaaaagaa gcgctgaatc tagaagaagt atcaggtgtg atgaagctca
    55501 tgctttacat cctaaaattg gcctttgtga tccaattgat tggtacggtt gctttatctt
    55561 ttgtatttgt gcctgaattt ggctggttga aaggtatctg gtacagtttg ttccatgcaa
    55621 tctctagttt ttgtaatgca gggtttgatt tgttaggaga cagtttagcc aatcaccagc
    55681 aaaatgttta tttgatcatg gtggtatctg ctttgatcat ttctggtggg ttaggcttca
    55741 tcgtatggcg tgatttactg gaatatcatc agaaaaagaa gatgacgatt cattcaaaaa
    55801 tcgcattaac cgttacaggt acccttttat taggcggatt cgttgtgttc tttttcacag
    55861 agcgcaatgg ggcaacgcta gtggaaggga atatgtttca acgattggca aacacctttt
    55921 ttatgagtgt gacacctaga acggctggat attattcagt tgattacctc catatgagcc
    55981 atgcaggtct gattttaacg atgtttttga tgtacatcgg cggaacatct gggtcgacgg
    56041 ccggcggatt gaaaacaact acgttaggtg tgttgctgat ccagatgcgt tcgatgttga
    56101 aaggacgtac gagagcggaa gcatttggcc gtaccatccg acaaggcgca gtgttgcggg
    56161 cacttacttt attttttgtt acgttgacgt tgtgtgtagt cgccatcatg attttatcgg
    56221 tgactgaaac gatacctaaa acttctggca tcgaatatat tgcttttgag gtcttctctg
    56281 cgtttggtac agtaggtctt acgatgggat tgacgccaga cttgactgca atagggaaat
    56341 tgatcattat agcgcttatg tacattggac gagttggtgt attgactgtg gtcttctctc
    56401 tgatgacaaa agcacaaaga acacaatcaa aatacaaata tccagaagaa agtgtcatga
    56461 ttggataatt tcatcttctg acagaaacct agtgatttag taaaataaga aaataaaata
    56521 atttttcttg cttcttggat actttaagtg caaaatgttc gtttattttt tgaggcacta
    56581 cccgttgttg aacctcgggt agtgcctctt ttttattgaa aggaatcaat aagatttgat
    56641 tcctttattt atcaataggt tataattcat atttttttat tttttctttt acgatactta
    56701 gtccagaagc taaaatttca aggttattag aggataggga aattcgaacg aatgcatctg
    56761 gttgcgtacg tgtagtaaag cgtctggaat gataaagtcg gataccgtta gcaagaaagt
    56821 cattctcaat tatcttaggg tcaatctcag gatcaactgg aagaattcga tagtaaggga
    56881 cacgattcgt tggtttttct actttgaaaa gagaatcaaa aagctgattg gctctttttg
    56941 taagacttaa tttagtttct aagatcctat ttgcttcatt tgaatggagg atttgagtga
    57001 tgacttccac atctaatcca gaagttttca catttatatt aaaaattgct tgttctatct
    57061 tgctttggac atgtactgga actacaagat aagcaactcg caatccagaa caaataaatt
    57121 ttgtcattcc agctaagtag attgtttggt taggtaataa attttggaaa ggaggaatga
    57181 tcgtatgttg atagtaagtt gttaaaaatg aatggatatc atcttcaata acccatagac
    57241 cttctttttc aatcacttcg gctaattgct ctcgtctttt gtgagagatt tgaaacccta
    57301 taggattatt acaagaaggc ataagaaaga caccatgaat cttctttttt ctacattcct
    57361 gaatcaatat atcaggtcgc attccttgct cgtcaaaatc aattggaacg atctctaaat
    57421 gatataactg agccaattca atgaaattag cgtatgtata tcggtctgtt gctattctat
    57481 cacctggaga gaaaagtgca gaaagtgtga tagccaatcc attttgaaca ccggaaacaa
    57541 tagatagaga ttctaaggac ggaatgactc cttgagtttt taaccaattt gctgcagtag
    57601 tcaattgaaa atttgtgcct ttgggatctt ggtaattaag caaaccaaca agatttgggt
    57661 tattactagt tgattgaata aacgggacaa cgaatttatt acattcttca aaagaactaa
    57721 ccagaccaaa atcaatgata tgttcggtta cttcattggt agagatggtg atggactcaa
    57781 agacattggg agaaataaat gtgccacttc caatattcgt atacagcaaa cctttctcaa
    57841 ttccatattt ataagccttg ccaacggtag taaaatttaa atctaaataa tcagctagct
    57901 ctctttggga gggtaactga gtgtttttct gtaatttatt ctttaaaata tcttgttcta
    57961 attgttgaat caacgatcga taataaggac gcttcaattt ttgtttgtct ggtcgccaag
    58021 ttaacgggta attgtcataa gaattaatag gcattttctc cactccttaa aattgtatac
    58081 catacaattt taccattgat tgtattgatt ttagaagtta atcttttgtg aggagggaat
    58141 tgtctatgaa aattattaat tttgaggaag tagtagcttc ttttagtttt gaagaagcga
    58201 ttcaagtaat gaaaaaatgt ttttttgatt atgaaagtgg taaaatcagc caaaatcctc
    58261 gattggtgga aattttacct gacggaaaag gcaaaaatat gtttgcgatg atgcctgctt
    58321 atttggggga gaacagatat tttggcacaa aagtcattac cgcttttccg gataatcatc
    58381 agtatcattt accttctcat ttaggagaga ttttcttgtt tgatgctcaa aacggactac
    58441 ccaaagcatt gataaacgca aatgcagtca cctggattcg aacagctgct gtatctgcgc
    58501 tggcaacaga ttacttagca aatccgcatg cagaaacact tgctttaatt ggagctggtc
    58561 aacaagcatt gtcgcatttg actgcgattt ctaccattcg tcccatcact acagtgtttg
    58621 tttacgatat tttagaagaa agacgaaata cttttatcgc taaagctaaa aaacagtatc
    58681 ctaaaataac gtttataaat gcaatatcag tagaagatgc agtacgtcca gcggaaatca
    58741 tttgtacgtt gacctctagt aaagaggcat ttcttcaaaa agagtgggta agggaagacg
    58801 cacatatcaa tgctgtgggc acatttactc ctgatacaag agagataagt acatcgttga
    58861 tggaaacttc tcaaatctat gtagatgatt actcagctgc cttaacagaa agcggggacc
    58921 tgcttatagc catggcagaa ggagatttag aggaatcttg tattgttggt acattaggcg
    58981 aattagtttc tgagaaaaaa acattttata aaggagaatc gaggccgacg attttcgacg
    59041 cagttggtct agctattgaa gatctttgtt gtgcagaata tatttataac aaattagaga
    59101 aaggggcttt aaaatgaact tagattatta cgtagttgat gcatttgcag atgaggtgtt
    59161 caaaggaaat ccagcagcgg tttatgtgct agaagaatgg ctgccagaag gtacgatgca
    59221 aaaaattgcc attgaaaaca atttatctga aacagcattt acagtgaaga aaaaccaaga
    59281 atttgagctt agatggttta cgccagatcg agaaatcgat ctttgtggtc acgcgacatt
    59341 agcaacagca tttgttttat ttaactatta caaaatacct gatgaaacga ttaaattttc
    59401 tactcaaagc ggtaatctat ttgtgacaag aattaaagat tattattaca tggattttcc
    59461 tagtattatg ccgaaaaaag tgccgatatt gtctgaatat gaagaagcga ttggggctaa
    59521 gattaaagaa gcctacttag cacgtgattt atttttcgta ttagaagatg aaaaaacggt
    59581 ggctaaattg cagccagatt ttacagctat caaaaatttt gaattaggaa taggagtcat
    59641 cgtgacagca gaaagtgtgc aaaaagactt tgtttctaga accttctttc caaaattaac
    59701 aatcaatgaa gatccagtat gtggatcagc acattctaat cttattcctt attgggcaga
    59761 aagaatgaat aaaacaaaat tgtcagctta tcaaatgtca tcaaggggcg gggatctaga
    59821 atgtgaatta aaagaagatc gtgtgataat aggaggaaaa gcagttttgt tttctaaagg
    59881 tacctcgttt atttgatttc tttcaaatga attaaaaata aattggtgga gagtggcaac
    59941 cttatagcta caatggttgt taaaaaaaga cgcaaacttt ttcaatttaa tgaaaagttt
    60001 gcgtctcatt ttaattattg gtgtttgttt tttattcaat caagagtaaa atgccccata
    60061 ctaactatcc tcggatgact tcgattttat aaccatcagg gtcaatgaca aagtagtaag
    60121 aaggcgcagt gccaggtaat ccttttaagt cagtaatctc aagaccagca gctttatgtt
    60181 gctcgtgtaa tttttctaga tcatcagctg ctatagcgat atgaccatag ccgtctccta
    60241 gatcatacgc accatgatca tagttgtaag tcaactctaa ttcatagtca tccccaggaa
    60301 gtgtcaaata aacaagtgta aatttgtttt ctggaaaatc acggcggcgg ctttcttcaa
    60361 aaccaaatgc cttttgataa aattcgattg atgcttcaag atctttcact cgaacacatg
    60421 tgtgagccat tttcatagat tcatctctcc ttttcctttt actcaatcat aacaaaaaga
    60481 agtaaggaaa tagttgattt gtgcttgttc atgagaaatt tctcatgtaa aatggataga
    60541 aaataaaact gttaaggtgg aaactattca tggataaacc aatttttggt gaaaaaaagc
    60601 aatttgctga ttatcaaacc cgttatgcag catatattgt catagaaaga gatcagcaga
    60661 ttgcagtaat cgaggctcca aatggtgctt tttttcttcc tggcggggaa attgaaggag
    60721 aagagacgaa agaagaagcg attgtccgtg agctgataga agaaatggga attgtggcag
    60781 aaatttcttc ttatctggga gaagctgatg agtattttta ctcgaattat cgtgcaactt
    60841 attattataa tccaggatat ttctttgttg ctgattcttg gaagcgtata ggagaaccaa
    60901 ctgaaaagac caataaaatt tggtgggtga gccctcaaga agcgcttgtg aaattgaaac
    60961 gtggtagcca ccgatgggca gtcgagagat ggatcgaaaa aaataaaaac tgaaagaaag
    61021 cgagtagatc agctttctct cagttttttt atttattaca gtacatattt tttgattgct
    61081 tcagctacac cgtgctcgtc gtttgatgaa gtgatgacat ttgctgcttc tttgacaaga
    61141 gaaacggcat ttcccattgc tactcctaga ccagcatatt caatcatgga taagtcgttc
    61201 tcgttatctc cgatagccat gatttcatct tgatcaatac caaggtgctg tgcgagattc
    61261 gcaactgccg cacctttact tgcttcttta tttagtactt cgaagtaata gggcgcactt
    61321 ttgaccgttg tgtatttatc acggaaagtt tgcggtaaac gcgcaattgc tgcatctaaa
    61381 atttctggtt cgtcgatcat catcattttg acgatgttca tgtctggtgt catttcttca
    61441 ggtgttcgat attttagcgg catcttgact aaacttgctt cgtggatcgt ataagcgctg
    61501 atatctcgat taggcgtgta gatcgctttg tcatcgatcg aatggagatg actgccgaca
    61561 cggcgtgcca tcacttcgat ctctaggaaa tcttcatgtg tcagtccgtg acgggcgatg
    61621 attttacctg atttcgtttg ttgtacgagt gccccgttat aagtaataac gtaatcatta
    61681 tcttgaaaaa ggttcaactc gtttaactgt tcctggacac ctggtaaggg acggccagtg
    61741 cataaaacga tcgaaacacc ttttgcaaca gcagcggcaa tcgcttcttt tacttctttt
    61801 gttacttctc ttttctcatt taataaagta ccatcgatgt cgatagcgac tagtttgata
    61861 gacataattt cctccaatat attttaattt atttcaatca atgcgccatt acggatatgg
    61921 ctggcaaatt cttgataagt ttcgtcaaat aaatcataat gatcacgcaa ggacggatca
    61981 accatttctt ttggaaagaa aaatcgctca tctccttgtt cttgtcccgc taatgcagca
    62041 acaagctgac taacctgtga taattctatc aaagttccgt catttcgcaa aatctcgatt
    62101 tgtgtgcgat gtctgccttg ttctgggcga taaaaatcat aaggtagatc gtagctagaa
    62161 ttcacagcag tgtaataaac aggattatat ccgacctttt ctactaacaa agtgagttct
    62221 tggactaatt ttgaatcccg gttgccagaa aaagtagcag atttcagtgg tttacgattc
    62281 aaaaaacgtt tagctaaatc atttaaaata ggatcagcag attcagacca ttgagtaaag
    62341 taagtgccca agaccccatc atcaagtttg aggtattctt ctaaagtaaa gcttccgtca
    62401 agaaatggca gaagtaattg agaatggagt tcaaaactgc tggcatcttc ttgatacaac
    62461 tcgtgtgcgc gatgaagcag atgctccaaa attacttcca taccacggga aacaggatgg
    62521 aagtacactt gcacatacat ttgatatcgg ctaacgatat aatcttccac tgcatgcatg
    62581 ccactcatgg aaaaagcgat cccgccttca taaggacgaa tcacacgtaa tattcgggta
    62641 agatcaaatg ttccatattc tgttccagtg aaataagcat cccgaagcaa atagtccatt
    62701 cgatctgcat cgatctgact agaaatcatt tggacaactt gtgggttggg gtaagttttt
    62761 tggatcacac tagctacctt ttcaggaaaa ccttcttcta ctcggttaag aatctgatac
    62821 acttctgttt ctggagaagt gatgatctct acagtgatgg cttcatgatt cgtatggaag
    62881 atatgttcaa atgtatgaga gtaaggacca tgaccgacat catgcaacag tgccgcacat
    62941 aaagtcacca gccgttcctg atcatcccag cctccgttgc ctattttttc ttttgaaaaa
    63001 tttcgagaaa agatatcgca gattcgtctt gaaatctcat aaacgcctaa tgaatgagta
    63061 aaacggctat gttctgctcc gtgaaaagtg aaagaagaag tgcctaattg tttaatgcgt
    63121 cgtagacgtt ggacttcttt cgaattgatc aaatctaaga ttacttgatg ctggacatgg
    63181 atatagttat ggactggatc acgaaaaact ttttccatag gcagcatttg atatttataa
    63241 ggggttgtca taatgtagct cctttatgtt attacgttgt tccattctct ggatttggtt
    63301 gttccattgc tccagctgga tttctttttt tagccagtcg gtagaatcct gtaaatcact
    63361 tgcagaatac agcttttcaa aagcttttgc aaatgcttct ttgacttctt ttacaaaaag
    63421 gggttggtca agcagttctt caagtgttgc cattgtagca ggatccactg caggatagcc
    63481 atttgtacca aagtcttctt ttaaagcaga ttgataaaat cgcttcatga cttgcccgcg
    63541 tttttcctga ttaccgttga cacttaagta catcataaca gaaatcccat ttttgatccg
    63601 acgctgagcg ataccggcaa attttttgcc atggatgctt aaatcataag ttccaggaca
    63661 ataagaatgg gtgatttcga atgcttgtat cggtgccttt ggaaaagcgt atttagtcaa
    63721 taggagcatt tgctcgtaag cgtcatctat ggttattttt tttgtgtcac tttgggggaa
    63781 aatcaaagaa atattcaaaa ttccttcatc tgatacgatc cctaatccac ctgaatttct
    63841 gatcactgga gcgtaacctg catctttcag tgtttggatc ccgtcactta gaaaaggagt
    63901 tcgaacatcc ttcataccta agatgactgt gcagtcatat tgccagaaat gtaaaaagat
    63961 ttgctggtgt tctttagaat attccgttaa tatatctgtt aatgcaaaag gggaaaagtc
    64021 gtttttttga taaatgtgct gatctagtcg aatcgcaggg ttcattcaac cggctccttt
    64081 gtataaattc atattttcta caatattata ctctaaaatc aaagagttgt tttggaaaaa
    64141 aggttttctg taaaaatgga caagttggtt gacaaaacaa aagaaactag gaacaatgta
    64201 gctatgagaa gtaaaggaga atgaataatg gaattatcac aaggaacacc gatttcaatc
    64261 aaattgcgta caaaagtaaa acagcaaggg gaagtgcaag atttttattt tgatttgaaa
    64321 gggcaaatgg tgacaattgg cgatacgctg tacatacgtt ataaagagat tcaggaggag
    64381 acgggcgatg agattcctgt aacgatcaaa ttagttccag atggtcatgt acagctgatc
    64441 cgtgcaggag aaatgcggat gcgtttgaaa tttggctata aagaacggtt agaaacatcc
    64501 tatcgaacac catacggtat gatccagatt gctactttta cgaaagaact gcatgttagt
    64561 ttgaaagacc gtcccacagc agggaaggtt cgaatcgatt atgatctgtt catgggaccg
    64621 gaaaaaatcg gcgaatatta tttgaccttg gattttacag cgtaaatata aaagaaaagt
    64681 ttgatttttt gtgtctgatt ttgtatgata aggagtagac tgtgaaagga cgtgtcctga
    64741 ttggaaatta gtgtatttga aggtgcaaac aaaagcgaat tatcaatgat cgaagtagca
    64801 cacgcgatct tagaacaacg tggcgatgtc atggattttt ctgatttagc taaccagatc
    64861 caaaactatc ttgggaaatc agacagtgaa attcgtgatt cactcgcaca attttatact
    64921 gacttgaata ttgacggcag ctttatctca ttaggggata atcgttgggg cttgcgttca
    64981 tggtatgcta tcgattcgat cgatgaagaa gtaaaccatg gaatggacga agaggatgaa
    65041 gatactccac gtcgccgtaa acgcaagaaa gtcaacgcct ttatcaatga tgatgaagac
    65101 gcgatcgatt acaatgacga tgacccagaa gatacagatc tgacagaaga agatgacgat
    65161 gatctgtttg atgacgatga tgatgaagat gaagaaatcg ctgcttataa ttctgacttg
    65221 caagaaatcg gagcagatga cccgacagat gatgaagaag acttgccagg aaatatcgaa
    65281 gaagatttaa gtatcataga cgaagacgat gatgatgaag attatgaaga ggaagaattt
    65341 tctgactctg aaaaagaata aacattattt tcttacgaga ggttgtgaca gagacgatca
    65401 gcctatcaga ataaggaggt gtgacacgat ttttgtcaca ccttcttttt tgtatataaa
    65461 aatcccaagt aaatacataa aaaataaaaa aattgaaact tttagcgcac cttgtacgtc
    65521 atattaagtg aggcagaaag gagtggagaa tgatttttac tggatataag aaaaaacggc
    65581 agatcgaaaa ataccgtcac ctgctgaatg gactcatcga aactgattat caaaaaatgt
    65641 tccgtatcac gatgaattac gtacatgata aagaagaggc cttagatgtg atgcaagata
    65701 gttttcataa agccctgaac tattttgaaa aagaaaaaga gatcgagcat tttagtgcat
    65761 ggttttatcg gatattgatc cgcacggcat tagatagttg gcgcagacaa aaaaggacgc
    65821 ctgtggactt atttgaaatt caagatatcc cccaacagtt acctttagaa acttccgttg
    65881 cagagctcca tatgatttta gataaaatag acagtccgga aaaagagatt ctgatcctct
    65941 acttttttga aggatttcgt ttgaaagaaa ttgcgactat cttggatatg aatgaaaaca
    66001 cagttaaaac gaaaatgtac cgctcattga atgctttacg tcaaatccta gaatgaagga
    66061 gagaagagtg ccatgtattc ccaaaaagaa aatttaaaaa aactaaagaa aacttataaa
    66121 gaacaagcaa ttcccaaagg gcttaaagag gagatccgcc agcgttttat tatcgaagag
    66181 caatcattta taaaagcaaa gcggagaaag agacgtctcc aagggttcgg agtatctttg
    66241 gctatcgtaa tgattacaac aagtagcctt ctttttaata gtcaagttcg aagctttgca
    66301 gaagatttac ccatcttagg atcagtcatt gaattgatct taggcgaacg ctttactgat
    66361 cgatctgaaa aaatcgatat tcaggtaccg aagatcaata cacaaaatga gaaagaaaat
    66421 aaaacaattc atggattaaa tcaaaaatac tttcgcgaag gacaagcaga ttttgaaaaa
    66481 gctgaaaaag aatatggaaa ttttgaaacg gatcattacc aagttttggg agattatcag
    66541 aaaatcgtag acgataaccg gtttctcgtg attgaaaggc agatcaccca aacagcagca
    66601 gatagccatg ttgaaaaaag atacgacaca atcgacaaga aaaacagtgt ccagctctca
    66661 cttccacttt tattcaaaga tgacacctat ctatctgttt tgacaaaaga agtcaaacgg
    66721 cagatggccg aacaagtcaa ggaagatcca tctaagtact attggactga gcaagatatt
    66781 caagaaggga cgatagaaaa accaacactt gttacgccta cacgaagttt ttatttgaac
    66841 aaagagcatc aattagtttt gactttttct caatacgaaa ttgctcctgg ttatatgggt
    66901 acaccagaat ttgtcattcc taagtccgtg acaaaaacga ttttggcatc ggaagattat
    66961 ttggatcatt agacgagaag tagttgcgac tagcagtttc aattttcaag taagtacaag
    67021 taaataataa gaagagaaaa agagaaagaa gagatatagt gaaaagaaat tatttagtta
    67081 tcagtattat tgctttatcc gtgcttttgt ttttcgaaat tacttcggtg gcctccttga
    67141 tcaccgaagt aaaaggacaa acacactctt cggagcattc gtcacaatct ttcaaacaat
    67201 cagaacaatc aaccattaaa agccaatcaa ctgaaacagc taacgtcaaa gaagccattc
    67261 aagaatcagc ggaaaaggta caaagtcagt catcacaatc aacacaagac agtacaaaaa
    67321 cgagccgaga tccggctgtc aaaagcgtcg caatcagctt tgatgatgga ccaggagcga
    67381 ccacaacacc gcagcttctg cgaatcttaa aagaaaaaaa cgtgcatgcc acttttttcg
    67441 tcttaggaga aaatacggcg cagcatcctg aaatcgtgaa gcagacagct gaagcagggc
    67501 acgagattgg caatcacaca tacgatcatc agaatctggc cactctttct gcccagtcga
    67561 tgacggagga agtgacgaaa gcagacactg agatcaaaaa agtcactggc aaaaccccca
    67621 cttttgttcg tccgccctat ggttctgtca cgaatgtagg agcaacgatc atccagcgac
    67681 cgatcatcga atggtctgtt gattcagaag attggaagac aagaaatcct gatttgattt
    67741 tgcagaagat ccaagcaaca gtatacgatg gggcaattat tttattccat gatatctatc
    67801 ctgaaacgat tcgtgccgtt cctcaagtga tcgattatct gaaagagcaa gggtatcgca
    67861 tcacgacagt tggggattta ttagggcatc caacggctgt cgagaactat tatggcagaa
    67921 acgatcatcg gccagttcag taagtgagac aagtatcttt aataaataac acattttaat
    67981 aagtagttga acacataact accttacttg caaaggctta acaatatcta tacaggtaca
    68041 acaaaaaact gtccaccaat tttctttggc ggacagtttt cacttaaaaa taaagaagca
    68101 actattcgta aattatgcta aaatagcagt agaacgcgtc ctgagggaat cgaacccccg
    68161 tctcaagaac cggaatctta cgtgatatcc actacactaa ggacgcaagc actgggataa
    68221 ttgtaactaa acagataaaa aattacaagg ggattggaca aaaaaatgaa atttgagcaa
    68281 aatttttcac aaaaacagca gcagacacag aaactagcca tgacgcaaca gttgcaacag
    68341 tccatccaag ttttacaata taacagtgag gagctgttgg cattcgttga gaatcaagca
    68401 atggaaaatc cattggtgga agttgttgag ccggaatggc agccagatta cataaaagct
    68461 tcttcatctt cttatgaagg agaagagaca aattacctaa atcagattcc tgatacaaaa
    68521 gggtctttat ttgattcatt gatcgaacaa gtccatttga attaccgaga tacttttttg
    68581 aggaaaatcg ttctttatct tgtagaatac atcgatttga acggattttt gacgattagt
    68641 ttagaagaag ccggaaaaga gattggagct acgcctatcc aaatgctcga tgcgttgact
    68701 ctgattcagc aattagaacc agctggagta ggtgcacgta gtttgcaaga atgtctcatg
    68761 cttcaaacag aacgagatga ttatgcgccg gaattagctt atatcgtgtt ggaagaatgt
    68821 tttgaggaac tggtggagcg aaaatggaaa gaaatcgcac aacgtttttc tgttgatcta
    68881 catgctgttc agcagatatt cgactattta caaacgttga gtccaagccc tggccggatt
    68941 ttcgaccgta gcagcgaatt gtacattatt cctgatgtcc gggtattagt ggacgacgat
    69001 aaaaatgtac aagttatctc aaaccgaaaa aatcagccaa atatacgctt tcaagagagt
    69061 tacttcaaac aaatgtcaca acaagcagat aaagaaactg aaaattattt aaaagaacgg
    69121 aaacaggaat tcgaatggtt gaagaaaacg atcctacaac gtggggatac aattttgcgg
    69181 gttgctcaag tcattgtttc ccggcaaaaa gctttcttta tcgacaagga acgtccaatc
    69241 aagcctctta cgttaaagga agttgcaact gagatagatg tgcatgaatc cacggtcagt
    69301 cgtgcagtca acgggaaata tttggaaaca gacttcggtg tatttgaatt aaaaaaattt
    69361 ttcactacac gaattcctac gaatagtaca gaacagacag aagacctttc agcagataca
    69421 gcgaagaaaa agctgcagga attagtcgat caagaagaca aaaacaagcc gttgtctgac
    69481 cagaaattgg ttgaattgct gaaaaaagat gagatagcga tctcaagacg aacagtagca
    69541 aaatatcgtg atttattagg aatcccgagt tcatcaaaaa gaaaacgtta tgataactaa
    69601 tcagtcaaaa gactagaaaa agtatttcac tttttctagt ctttttagtt gaaaaacgag
    69661 agcaaaactg ttagacttac agtcgtgagg ttgagatttc cctttattga tgtgaaattc
    69721 gaatagttaa tttctttttt tatcgtatat gggtcgtttt aagtctacat tggacgtaaa
    69781 gtgtccaact aaggagatcg ctatgctgga tgaaataaaa atgattgaat ctgttgcacc
    69841 agatattatc gaagtgttgc aagaacgcta taaaatttta cgaaatatct attggatgca
    69901 gccgattgga cgtcgaagtt tatcggaaag tatggggatc acagaacgag tattgcgaac
    69961 agaaacagat cttttgaaaa atctgaattt gatcgacacc tcaaaaagcg gtatgacgct
    70021 gacaactaag ggcgaagaag tttatcaaag ccttgaaaac ttcatggatc agcttttagg
    70081 tacgcatcaa accgaacaac agttggcaga atattttggt aaccaacgct gtatcgttgt
    70141 ttcaggaaac agcgaagagc agaccaaagt ggctgatgca ttcggcgaag cgctgagcga
    70201 agccctagac cgactacttc cagaaggcga gaatatcatc gctgtgatgg gaggaactac
    70261 tatggctgtg gtagccgaac aattgtctaa tctggaaaat aagaaacggc ataacctgtt
    70321 tgtccctgca agaggcggga ttggtgaagc gataactgta caggcaaatt ctgtcagtgc
    70381 aagaatggct gctaaagcaa acggtaacca ccgtgcactt tatgttccgg aacaattaag
    70441 tctggcaact tacaattctt tactcaatga gccatctatc caagaagtct taaacttgat
    70501 tagtgaagcg aattgtgtta ttcatagtat tgggcgtgca ttacacatgg cagcacgtcg
    70561 taagatgact gaaaaagaat tagtcatgct gaaacaagca aatgctgttg cggaatcttt
    70621 tggctacttt ttcaatgaaa aaggtgaagt cgtttataag gtccctagaa tcggcataga
    70681 attgcaagat ttggaaaaag tcccgatcat catggcaatc gcgggcggaa aatcaaaagc
    70741 taaagcaatt cgagcttata tgaaaaatgc accaaaacaa acgtggctca tcactgatga
    70801 agctgctgca aatgagattt taaaaggggt aaccctttaa aataaatatt tttttgattt
    70861 tcataaggag gaaatcttaa atgacagtta aagtaggtat taatggtttt ggacgtattg
    70921 gacgtctagc attccgtcgt atccaagatg tagatggaat cgaagtagta gcaatcaacg
    70981 acttgacaga tgctaaaatg ttggcacact tgttaaaata cgacacaact caaggacgtt
    71041 tcaacggaac agttgaagtt catgaaggtt cattcaacgt aaacggaaaa gaagttaaag
    71101 tattagctaa ccgcaaccca gaagaattac catggggcga actaggcgta gatatcgttc
    71161 tagaatgtac tggtttcttc acttctaaat cagcagctga aaaacattta acagctggtg
    71221 ctaaacgcgt tgttatctct gctcctggcg gaaacgatgt tccaacaatc gtttataaca
    71281 caaaccacga aacattaact ggtaaagaaa cagttatctc aggtgcttca tgtactacta
    71341 actgtttagc tcctatggct aaaacattga acgacaaatt tggtgttgtt gaaggattaa
    71401 tgacaactat ccacgcttac acaggtgacc aaatgactct agacggacct catcctaaag
    71461 gtgacttccg ccgtgcacgc gctgctgctg caaacatcgt tcctaactca acaggtgctg
    71521 ctaaagctat cggtttagta attcctgaat tgaacggtaa attagacgga gctgctcaac
    71581 gtgttcctgt accaacaggt tcattaacag aattagtaac agttcttgaa aaagaagtaa
    71641 ctgttgacga aatcaatgca gcaatgaaag aagcttcaaa cgaatcttac ggatacaaca
    71701 cagacgaaat cgtttcttca gatatcgttg gtatgactta cggttcatta tttgatgcta
    71761 cacaaactaa agtaatgaca gttggcgaca aacaattagt taaaactgtt gcttggtacg
    71821 acaacgaaat gtcatacact gcacaattag tacgtacttt agaatacttc gctaacttat
    71881 aagatagaca aacgtacaag tgtaaattat agacatgtac atttaaaagc ggggaagcaa
    71941 tcgcgcttct tccgcttttt ttgataataa gtttattcag gaggaaataa aatggctaag
    72001 aaaacagtga aagatatcga tttaaaagac aaaaaagttc ttgtccgtgt tgacttcaac
    72061 gttcctttga aagacggcgt gatcactgac gacactcgta tcaaagcagc attgccaaca
    72121 atcaactacg ttctagaaca aggcggaaaa gcaatccttt tctctcacct tggacgtgta
    72181 aaaacagaag aagataaaga aggaaaatct ttagctccag ttgcaaaacg tctaggcgaa
    72241 ttattaggta aagaagtaac attcgtacct gaaactcgcg gagaacaatt agaagaagca
    72301 atccgcaaca tgaatgacgg cgacgttgtc gtattcgaaa atacacgatt tgaagatatt
    72361 gacggtaaaa aagaaagcaa aaatgataca gaactaggta aatactgggc ttcattaggc
    72421 gatgtatttg ttaacgatgc tttcggtaca gctcaccgtg cacatgcttc taacgtaggt
    72481 atcgcttcaa ctggtatccc aacagttgct ggattcttga tggaaaaaga aatcaagttc
    72541 atcggtgaag cagtagaaga accaaaacga ccaatgattg caatccttgg tggcgcaaaa
    72601 gtttctgata aaatcggcgt aatcgaaaac ttgattccta aagcagataa aatcttgatc
    72661 ggtggcggaa tgacttacac attctacgaa gcaaaaggaa tcaagatcgg taactctcta
    72721 gtagaagctg ataaagtgga actagcaaaa gaattaatcg aaaaagcagg cgacaaactt
    72781 gtcttgccaa tcgacaatgt ctgtgcacca gaattttcaa atgatgttga aacacaagta
    72841 atcgaaggag acattcctga tggtttgatg gctttagaca tcggcccagc atcagtaaaa
    72901 ttatttgctg atacattaca aggtgcaaaa acagttgttt ggaacggacc tatgggtgta
    72961 tttgaaatga gtaactttgc taaaggtaca atcggtgttt gtgaagcaat cgctaactta
    73021 gaaggcgcaa caacaatcat cggtggcgga gactcagccg cagcagcaga acaattagga
    73081 tttgctgaca aattcacaca catctcaaca ggtggcggcg caagcttgga attgcttgaa
    73141 ggtaaagaat taccaggact agcagcaatc aacgataaat aaacgaccaa gtttgctcaa
    73201 ttctgacgag taatcgaaca aaaggtgaag ggagttttca tgactcccta gccatttttc
    73261 aataaattaa ggagtgtgtt cctcaatgcg taaacctatt atcgcaggta actggaaaat
    73321 gaacaaaact gcatcagaag caaaagaatt tgctgaagca gtaaaaaaca aaatcccttc
    73381 aaatgacaaa gttgattcag tcatcggatc tcctgcatta ttcttacaag aattaatgac
    73441 atcagctgaa ggtacagaac taaaaatctc agcacaaaac tgctactggg aaaactcagg
    73501 tgcgttcact ggtgaaactt caccagcagc tcttgctgat ctaggtgttc attatgtgat
    73561 catcggacat tcagaacgtc gtgaatattt ccatgaaaca gacgaagaca tcaataaaaa
    73621 agcgaaagct atctttgcaa atggtatgac accaatccta tgctgtggcg aatctttaga
    73681 aacctatgaa gcaggcaaaa ctgctgaatg gatcgaagga caaattacag ctggtttagc
    73741 tgatctttca gctgaacagg tgtcaaacat ggttattgct tatgaaccaa tctgggcaat
    73801 cggtactggt aaatcagctg acgcacaaat cgctgacgaa atttgtggag ttgtacgtca
    73861 aacagttgaa aaactttatg gaaaagaagt atcagaagca gttcgtatcc aatacggcgg
    73921 ttctgtaaaa ccagaaaata ttgctgaata catggcaaaa gaaaatgttg acggtgcttt
    73981 agtcggtggc gcaagcttgc aagcagattc attcttgtca ttgttagacg ctgtaaaata
    74041 attgttgaaa atttgcgaaa aatgcaagaa atgtttgcac taatgagcaa aattaactaa
    74101 aatcgtatat gagggaaatc ccttaggaaa aaacaaactc aaaggagaga caaaacatgt
    74161 caattattac tgatgtttac gctcgcgaga tcttagactc acgtggtaac ccaacaatcg
    74221 aagtagaagt atacacagaa agcggagctt ttggccgcgg aatggttcct tcaggtgctt
    74281 caactggtga atacgaagca gttgaattac gtgacggaga caaagctcgt tatggcggta
    74341 aaggtgtaac taaagctgtc gacaacgtaa acaacatcat tgctgaagca atcattggct
    74401 acgatgttcg tgaccaaatg gctatcgaca aagctatgat cgctttagac ggaactccta
    74461 acaaaggtaa attaggtgcg aacgctattt taggtgtttc tatcgctgtt gcacgtgcag
    74521 ctgctgacta cctagaagta cctttatacc actacctagg cggattcaac acaaaagtat
    74581 tgccaacacc aatgatgaac atcatcaacg gcggatcaca tgctgataac tcaatcgact
    74641 tccaagaatt catgatcatg cctgttggcg ctccaacatt caaagaagct ctacgtatgg
    74701 gtgctgaagt attccacgca ttagcttcaa tcttgaaagc tcgcggatta gcaacttctg
    74761 ttggtgacga aggtggattt gctccaaacc ttggttcaaa cgaagaaggt tttgaagtaa
    74821 tcatcgaagc aatcgaaaaa gctggctatg tacctggtaa agacgttgtt cttgctatgg
    74881 atgctgcttc ttcagaattc tacgacaaag aaaaaggtgt ttacgtacta gctgattcag
    74941 gcgaaggcga aaaaacaact gacgaaatga tcaaattcta cgaagaatta gtttctaaat
    75001 atccaatcat ctcaatcgaa gacggattag acgaaaacga ctgggacgga ttcaagaaat
    75061 taactgacgt attaggcgac aaagttcaat tagttggtga cgacttgttc gtaacaaaca
    75121 ctcaaaaatt atctgaaggt atcgaaaaag gaattgctaa ctcaatcctt atcaaagtga
    75181 accaaatcgg tacattaaca gaaactttcg aagctatcga aatggctaaa gaagctggct
    75241 acacagcagt tgtatctcac cgttctggtg aaacagaaga ttcaacaatc tctgatatcg
    75301 ctgttgcaac aaacgctggc caaatcaaaa ctggttctct atcacgtact gaccgtattg
    75361 ctaaatacaa ccaattacta cgtatcgaag accaacttgg tgaagttgca gaatacaaag
    75421 gtttgaaatc tttctacaac ttaaaaaaca aataattctt ttctaatgaa gatacatgat
    75481 cgactattga aagatagtcg agtatgatca cttagttaag taatcgaact ctctacttgc
    75541 tgaaatatca gcaggtagag agtttttttg caactagctt ctgtatatga tgtttacaaa
    75601 gaatggtaca agaaaagatc atccttgaaa aaggaaagct aacaataact agcatcatat
    75661 aagagctttt aaggatagaa cagtagaata ggaatctatg agcaagtcct ctcgtataaa
    75721 taaaatagga gggaagtagc agcatatttt tcataaagtg gctctctttc ttcattttta
    75781 ctttggttgc tcctatttga aaattcgagc acactcaaat acaataaaaa gaaatggcaa
    75841 gggagaggat tagatgaaaa aagaagaaaa aatcgcgatt ttacaagaga tcatccgaat
    75901 taaatcagtg aatggaaacg aaggtgaagt agcagcctat ttgaataagc ttttggcaaa
    75961 gcatgacata acaggagaga ttgtttctta cagagatggt agagataatt tgattgctcg
    76021 ttaccaaaag ggccaatcag gaaaagtgtt aggcctatct gggcatatgg atgtcgtggc
    76081 ggctggtgat gaatcgtctt ggacctatgc accctttgca gcagaaatcc atgggaaccg
    76141 tttgtacgga cgtggtgcta cagatatgaa gagtggcttg gcagcaatgg tgattgccat
    76201 gatcgaactg aaagagtcag gaaaaccatt taacggaaca gtcaaattat tagccactgt
    76261 cggagaagaa gtgggtgaac taggtgggga acagctcacg aaagcaggct atgtagatga
    76321 cttagatgcc ttgatcatcg gagagccaac caattacagt ttgatgtata cgcacatggg
    76381 atcaatcaac tatacagtca cctcgcacgg aaaagaagcg catagctcta tgccagacca
    76441 aggttataat gcaatcaatc acctaaatga attcattacg aaagcaaatg ctgaaatgaa
    76501 tcacctcgct gaaacaatcg agaatcctgt cctaggaaaa acaatccata atgttacatt
    76561 gatcagtggc gggaatcaag tcaacagcat cccaagccat gcgcagctcc aagggaacat
    76621 tcgatcgatt ccagaatacc caaatgataa aatcattgca ttgctgcaat caatcgtcaa
    76681 tgaattgaat caagagacag actatcattt ggaattgatg atcgattaca acaaaatacc
    76741 tgtcaaagct gatccagatt ctcctttgat tcattgcatt cagcaacaat tcagccagcc
    76801 attaccttta gttggagcgg cagcaacaac ggatgcagcc gaatttacga aagctaacca
    76861 ttccttcgac tttgtcgtct ttgggcctgg tgtcgttact ttgcctcatc aagtagatga
    76921 gtatgttgaa atcgataatt atttggatat gatcgaaaaa tatcaaggaa ttattttatc
    76981 ctacttagcg taagattttt ttaataaatg aaaaaacaga gccagattag catta
//
LOCUS       DAYYGO010000002        64641 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000002 DAYYGO010000000
VERSION     DAYYGO010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 64641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64641
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.002"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(61..726)
                     /locus_tag="ACHA5T_000079"
     CDS             complement(61..726)
                     /locus_tag="ACHA5T_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFM8885690.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(873..1478)
                     /gene="dhaL"
                     /locus_tag="ACHA5T_000080"
     CDS             complement(873..1478)
                     /gene="dhaL"
                     /locus_tag="ACHA5T_000080"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFM8885691.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(1506..2516)
                     /gene="dhaK"
                     /locus_tag="ACHA5T_000081"
     CDS             complement(1506..2516)
                     /gene="dhaK"
                     /locus_tag="ACHA5T_000081"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFM8885692.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(2491..2886)
                     /gene="dhaM"
                     /locus_tag="ACHA5T_000082"
     CDS             complement(2491..2886)
                     /gene="dhaM"
                     /locus_tag="ACHA5T_000082"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFM8885693.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            3040..3360
                     /locus_tag="ACHA5T_000083"
     CDS             3040..3360
                     /locus_tag="ACHA5T_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFM8885694.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(3472..4329)
                     /locus_tag="ACHA5T_000084"
     CDS             complement(3472..4329)
                     /locus_tag="ACHA5T_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM8885695.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(4437..5741)
                     /locus_tag="ACHA5T_000085"
     CDS             complement(4437..5741)
                     /locus_tag="ACHA5T_000085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8885696.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(5738..6247)
                     /locus_tag="ACHA5T_000086"
     CDS             complement(5738..6247)
                     /locus_tag="ACHA5T_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8885697.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(6422..8557)
                     /locus_tag="ACHA5T_000087"
     CDS             complement(6422..8557)
                     /locus_tag="ACHA5T_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFM8885698.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(8640..10295)
                     /locus_tag="ACHA5T_000088"
     CDS             complement(8640..10295)
                     /locus_tag="ACHA5T_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8885699.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(10297..10611)
                     /locus_tag="ACHA5T_000089"
     CDS             complement(10297..10611)
                     /locus_tag="ACHA5T_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM8885700.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            10781..11041
                     /locus_tag="ACHA5T_000090"
     CDS             10781..11041
                     /locus_tag="ACHA5T_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFM8885701.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            11055..11369
                     /locus_tag="ACHA5T_000091"
     CDS             11055..11369
                     /locus_tag="ACHA5T_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFM8885702.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(12099..12653)
                     /locus_tag="ACHA5T_000092"
     CDS             complement(12099..12653)
                     /locus_tag="ACHA5T_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFM8885703.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(12779..14708)
                     /locus_tag="ACHA5T_000093"
                     /pseudo
     CDS             complement(12779..14708)
                     /locus_tag="ACHA5T_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(15014..15730)
                     /locus_tag="ACHA5T_000094"
     CDS             complement(15014..15730)
                     /locus_tag="ACHA5T_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFM8885704.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(15731..17554)
                     /gene="glpO"
                     /locus_tag="ACHA5T_000095"
     CDS             complement(15731..17554)
                     /gene="glpO"
                     /locus_tag="ACHA5T_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFM8885705.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(17568..19064)
                     /gene="glpK"
                     /locus_tag="ACHA5T_000096"
     CDS             complement(17568..19064)
                     /gene="glpK"
                     /locus_tag="ACHA5T_000096"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFM8885706.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(19200..20660)
                     /locus_tag="ACHA5T_000097"
     CDS             complement(19200..20660)
                     /locus_tag="ACHA5T_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8885707.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(20650..21945)
                     /locus_tag="ACHA5T_000098"
     CDS             complement(20650..21945)
                     /locus_tag="ACHA5T_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8885708.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(22277..22954)
                     /locus_tag="ACHA5T_000099"
     CDS             complement(22277..22954)
                     /locus_tag="ACHA5T_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFM8885709.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(22984..24075)
                     /locus_tag="ACHA5T_000100"
     CDS             complement(22984..24075)
                     /locus_tag="ACHA5T_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFM8885710.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(24176..24610)
                     /locus_tag="ACHA5T_000101"
     CDS             complement(24176..24610)
                     /locus_tag="ACHA5T_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFM8885711.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(25194..26651)
                     /locus_tag="ACHA5T_000102"
     CDS             complement(25194..26651)
                     /locus_tag="ACHA5T_000102"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8885712.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(26648..28543)
                     /locus_tag="ACHA5T_000103"
     CDS             complement(26648..28543)
                     /locus_tag="ACHA5T_000103"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM8885713.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(28601..30376)
                     /locus_tag="ACHA5T_000104"
     CDS             complement(28601..30376)
                     /locus_tag="ACHA5T_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFM8885714.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(30426..31922)
                     /locus_tag="ACHA5T_000105"
     CDS             complement(30426..31922)
                     /locus_tag="ACHA5T_000105"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8885715.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(32116..33738)
                     /locus_tag="ACHA5T_000106"
     CDS             complement(32116..33738)
                     /locus_tag="ACHA5T_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFM8885716.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(34066..35379)
                     /locus_tag="ACHA5T_000107"
     CDS             complement(34066..35379)
                     /locus_tag="ACHA5T_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFM8885717.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(35624..35848)
                     /locus_tag="ACHA5T_000108"
     CDS             complement(35624..35848)
                     /locus_tag="ACHA5T_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8885718.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(35868..36290)
                     /locus_tag="ACHA5T_000109"
     CDS             complement(35868..36290)
                     /locus_tag="ACHA5T_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8885719.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(36432..37142)
                     /locus_tag="ACHA5T_000110"
     CDS             complement(36432..37142)
                     /locus_tag="ACHA5T_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFM8885720.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(37160..37657)
                     /locus_tag="ACHA5T_000111"
     CDS             complement(37160..37657)
                     /locus_tag="ACHA5T_000111"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8885721.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(37694..38146)
                     /locus_tag="ACHA5T_000112"
     CDS             complement(37694..38146)
                     /locus_tag="ACHA5T_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8885722.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(38281..38886)
                     /locus_tag="ACHA5T_000113"
     CDS             complement(38281..38886)
                     /locus_tag="ACHA5T_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFM8885723.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(39336..39956)
                     /locus_tag="ACHA5T_000114"
     CDS             complement(39336..39956)
                     /locus_tag="ACHA5T_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8885724.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(39958..40827)
                     /locus_tag="ACHA5T_000115"
     CDS             complement(39958..40827)
                     /locus_tag="ACHA5T_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFM8885725.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(40850..41257)
                     /locus_tag="ACHA5T_000116"
     CDS             complement(40850..41257)
                     /locus_tag="ACHA5T_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFM8885726.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(41276..42565)
                     /locus_tag="ACHA5T_000117"
     CDS             complement(41276..42565)
                     /locus_tag="ACHA5T_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8885727.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(42592..42894)
                     /locus_tag="ACHA5T_000118"
     CDS             complement(42592..42894)
                     /locus_tag="ACHA5T_000118"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8885728.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(42919..45012)
                     /locus_tag="ACHA5T_000119"
     CDS             complement(42919..45012)
                     /locus_tag="ACHA5T_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8885729.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(45501..46265)
                     /locus_tag="ACHA5T_000120"
     CDS             complement(45501..46265)
                     /locus_tag="ACHA5T_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFM8885730.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(46376..47245)
                     /gene="mdcB"
                     /locus_tag="ACHA5T_000121"
     CDS             complement(46376..47245)
                     /gene="mdcB"
                     /locus_tag="ACHA5T_000121"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFM8885731.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(47270..48346)
                     /locus_tag="ACHA5T_000122"
     CDS             complement(47270..48346)
                     /locus_tag="ACHA5T_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFM8885732.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(48370..48963)
                     /locus_tag="ACHA5T_000123"
     CDS             complement(48370..48963)
                     /locus_tag="ACHA5T_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFM8885733.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(48967..50643)
                     /gene="mdcD"
                     /locus_tag="ACHA5T_000124"
     CDS             complement(48967..50643)
                     /gene="mdcD"
                     /locus_tag="ACHA5T_000124"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFM8885734.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(50633..50932)
                     /locus_tag="ACHA5T_000125"
     CDS             complement(50633..50932)
                     /locus_tag="ACHA5T_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFM8885735.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(51034..52680)
                     /gene="mdcA"
                     /locus_tag="ACHA5T_000126"
     CDS             complement(51034..52680)
                     /gene="mdcA"
                     /locus_tag="ACHA5T_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HFM8885736.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(52687..53598)
                     /locus_tag="ACHA5T_000127"
     CDS             complement(52687..53598)
                     /locus_tag="ACHA5T_000127"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFM8885737.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            53718..54575
                     /locus_tag="ACHA5T_000128"
     CDS             53718..54575
                     /locus_tag="ACHA5T_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8885738.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(54762..55124)
                     /locus_tag="ACHA5T_000129"
     CDS             complement(54762..55124)
                     /locus_tag="ACHA5T_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFM8885739.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(55313..55495)
                     /locus_tag="ACHA5T_000130"
     CDS             complement(55313..55495)
                     /locus_tag="ACHA5T_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8885740.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            55470..56909
                     /locus_tag="ACHA5T_000131"
     CDS             55470..56909
                     /locus_tag="ACHA5T_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8885741.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(56916..59468)
                     /locus_tag="ACHA5T_000132"
     CDS             complement(56916..59468)
                     /locus_tag="ACHA5T_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFM8885742.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(59582..60913)
                     /locus_tag="ACHA5T_000133"
     CDS             complement(59582..60913)
                     /locus_tag="ACHA5T_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8885743.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(60879..62357)
                     /locus_tag="ACHA5T_000134"
     CDS             complement(60879..62357)
                     /locus_tag="ACHA5T_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8885744.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(62662..64497)
                     /gene="tdc"
                     /locus_tag="ACHA5T_000135"
     CDS             complement(62662..64497)
                     /gene="tdc"
                     /locus_tag="ACHA5T_000135"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFM8885745.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
BASE COUNT        18402 a        13867 c        10966 g        21406 t
ORIGIN      
        1 aaaccttcac tcgcaagccg agagtaaggt ttgtttttcg cattgcttat ttcgtgttgt
       61 ctattgaaat ttttccgcac ctagcttttg atatagtggt gctagttctt gtaaggtttc
      121 agcggttatt tgctcaacgt gttcgatttc ttgcggatag aagaatagtt tacccatttg
      181 aaaggttcgg tctctcatcg cttttttcaa ctcttcttgt ttctttgtat cggacaaata
      241 gtcagacaaa gctaagtggt cgtcaagttc atgttctggt tgcggccata tgtaataatc
      301 cttgatatcg actgttttgc tccactcttt cagtatcgca agcaactggt tatacgctgc
      361 aatgctacca tatcgttctt cgcttttata atgttggtac atcaaataga tttggtattg
      421 tttttgattc aacaaaactg ctagacaggc attctcgtct tgacgagatt cgcttcggta
      481 cgcgctccaa aaatgactgc gtaaattcca gccattcgtc cagctttcga ttttaggttt
      541 gctcatgccg tacatatcag gcaaaagtgc agctgtctgg agctgaatct ctttccattt
      601 ttgccagtgt tctttatatt ctttcttgat ttgttcaatc ttatctgttg gatggctctc
      661 tttgagctgt ttgaatgcgt agattggtcg atcaaacaac tgataatctg attttccaag
      721 ttccattgtt tcgcctcttt ccgttatctt ttgtattcta cctgaatttt gcgatatgga
      781 caaatagagg ttgtgaaaaa gctgttctgc atcaagtcat aagaacagcc aaacaaaaaa
      841 ctgcgatgag catcaccgca gttttattga acttaaggtt gattgatcac atctaaaagc
      901 gttgaaaata aaatggcact agaagcagct cctgggtcga tatgtccgat tgcacgctct
      961 cctagatagg aagctcgtcc ttttttagct accaagactt tggtgtgttc ttttgcctgg
     1021 tcaatgattt cttttgttaa attccctgcc ttcaatgcct ctgtgactgg cagccaaaca
     1081 tctaacatcg ttttatcgtc agcttctgcc tttcctcggc tttgaatacc cgctgttccc
     1141 tgctcgatca cttcgcctaa tttttcttga gaatcaatag aatcgacctc ttttgtcgct
     1201 ttcatcatgt tcatgaatgc agatccgtat aacggaccag aagccccacc gactttcgat
     1261 atcatcgcca tagagaatac tttgaatgta tcgctgattg tttcaggatg atcggcttga
     1321 aaagcttctt cataagctga aacccctctt cccatgttat ttccatggtc tccatcgcca
     1381 atcggggtat ctagatcact gagatattgt ttgttctgat tgattgcctc tgcaaattgc
     1441 gtgagccatt cttggatttc ttgaacggtt agtttcatat tatcaccctt ttatcttgag
     1501 attttttacc atgaaatcgt ctgtacattg ctttctaaag aatctttcca ttgtgtctcc
     1561 gtcaagtcga tcagtgttag agaaagacct tgcatatcta gtgaggtcat aaaattaccg
     1621 acttttcgga aaactacatt gacgcctctt tcttccaaca gagctaaaac atcattcatg
     1681 aacacgaact gttccatcaa aggcgtcccg cccattccgt tgactagtac acctgcttct
     1741 tttggtaaat tcgagtaatc atctaaaatc ttagtaacaa gttcctttgc cagtacttta
     1801 gaaggctgca tcttttctcg acgatagcct ggctcaccgt ggataccaac accatactcg
     1861 atctcatctt ctcctagttc gaatcctggt ttccctactt ctggtacagt tgctgctcga
     1921 agcgctagac cgatcgtctt agttgcctgt acgacctttt ctcccagttc tttcaactca
     1981 gctagggaag caccgtttct tgctgcatcg cccacgattt tgtgtacaag gacagtacct
     2041 gcaactccgc gttttccagc agtatacgta ctattttcaa cggcgatatc atcatctacg
     2101 atcacacttt ctacttcaat atcgtccata gcagctaatt cctttgccat atcgaagttc
     2161 aatgcatccc ctgtataatt cttaacgatc agcaaaactc cttttccact atcggcttcc
     2221 ttaatagccg tctgaatctg atctggagtt ggagaagtaa acacatcacc aagcaccgct
     2281 gcacttaaca tcccttcccc tacaaacccg gcatgggatg gttcatgtcc gcttcctcca
     2341 ccagaaacca aaccaacttg ttccgctttc tgattcttgg ctacaacacg ggaagattct
     2401 acacgatgaa caagttctgg atagcttttt actaatcctt ccagcatttc ttcgacaata
     2461 ttgcttggat cattcatgat ttttttcaat ttattcccat cctttctttc cggcattttg
     2521 tgcttctgtt aagatttgct gagtatcatc tgttataccg gcagtgatgg ctgctgcaaa
     2581 agctccttcc actaaaggcg catccaccag atgataacgt tgctgctgct cttcttccaa
     2641 catgtctttt gccagttccg agttcaatac cgcgctaccg atatccgcaa aaacaaaata
     2701 actagctgca tcgcttgatt cattgactgc ttctacgatt ttcatcgggt ctgatccgat
     2761 ctcgccattt attccgccga gagaaaaaat ttgcacctct tcggattttt gcatctgctc
     2821 aatcatttct ttaataccat ctgttacttt ttgactatga gaaataagca aaatactttt
     2881 tttcacaaga atcttccttt cttttacata gttgctaacg attggtttcg ctttcattaa
     2941 tgaacaattc aagtatataa taaatttcta attttgggaa aatatgtgta gtgcttcttg
     3001 aagtttgtta tactttaatt aaaagagagg agtgatcgaa tgattacaac aacaacgaac
     3061 agtgtagagg gaaaagccgt agtagcttat aaagggatcg tttttggtga ggtcattact
     3121 gggatccatg catttaaaga cttggaggcg gggttgcgaa atatctttgg cggccgctca
     3181 aaaagctatg aaaacgaact aatgggtgca agagaagaag cacttgccga aatggccgaa
     3241 cgtgcaaaag cattaggagc tgatgcgatc attggcgtaa aaatggacta tgaagtatta
     3301 ggcagcgaca atggcatgct gatggtcaca tgtagtggaa cagctgtctc cacgagataa
     3361 gtaggggaca tctgttttaa taactaaaga gagatgggac atgtagtggc actcaagaat
     3421 tagatttttc agtctaactt cgaggtactc taccttccca tcctctcttt tttatttctt
     3481 tttgcggatg attccgtaag ctgctgcgac taacacaccc gcccctgcta aggaataact
     3541 gacagaagct gttttttcat ttgtttttgg caaagtctga cttgttgatt tggatggctc
     3601 tgtcgtttgc tgaactgttt ctgtcgtgct tccagaggtc actgattttt ctcctgccga
     3661 atgcaaaata tcttttattc gttggaaact agctgctaag tcagcttttt ttgcttcaaa
     3721 atcattagca actgtttgtg cttcgctgat ctttccgtgg attgcttgac tggccgctgt
     3781 gatatccgct tgagtttgac ttagctttcc agaagctgtt tgagcaacct gatttaattg
     3841 ctgattgaat tcttttgctt gttggatctt cccgtcaaga tctgtttgga cagcttccgc
     3901 ttttgctttc gcttcggcaa gctttttctc aagttgtgca cggtcatttc ccactttatt
     3961 tttaagatca gctaaatctt gatcgattgc tgcttggtct tctttcaatt tgttttttgc
     4021 agcttctaat gcttcttcga gttcttttct tttattttcc aagtcttgca aatgatcgat
     4081 ttggtcagaa gaggtagaac tatttattga tgtatcttct gaagatgaaa tcgtttctcc
     4141 tgaattatct gtcggtacat tggaagtcat ttctgtttcc gaggcaccgt tttcagcacc
     4201 agaagagctt atggttgttg aaggttctgt ttgagcaggt tcagaagttt ccgcaaaagc
     4261 tgtctgtccc aataagaaaa tactggaaac agcaattggc cagattaacg gcttcattct
     4321 ttttttcatc acttttccca tccttactac tttttaacta atttcttatt ataagcaaaa
     4381 atgtttggaa gaaccataaa cacaacaggc tgtttgtcta aaaaacggtg catcttttat
     4441 ttttttgtac gttccgggct tttccatgtc ccgcttccat aacctaaaat cgttttgact
     4501 gctggaatga aagtcgtcac gatcgttatc gcgttcacta tccagaacaa aagcatatat
     4561 agtggtgcga aaagaaggta tttcaatttt cttcgtcgat catctacaat cagcgaagct
     4621 agcaactgga aaaatcctgc gaccatttca aagcagatga acacaaaact gaatgtaaaa
     4681 gtaatataaa tctgttcgta gtttccttga tatacataat gcccgattaa tccagcaaaa
     4741 agaactacgg aaatacaaaa gaatattgac cagacaatac tcaatgtttg atcaatgaag
     4801 atgatcgttc ttccaatatg tttaaaagga tggagcatca cttttttgaa attcgtcagc
     4861 cagacttctg ttccaccttt tgcccaacgt tttctgtgtc gatatagcat cttcaacgtc
     4921 acaggtactt ccatgaaaaa gataatctca ggagcaaaaa cagataacca gtcatttaac
     4981 tgatgatccc aagcaatact gatgtcttct gtcgctcgat cttgtctgaa tccgcccaca
     5041 tcgatcaagg cttcttttcg atacatcgta tttgctccac tgtacgcata aagaccaccg
     5101 aaaacagctg actgtgttcg tttaatgatg cccacgatac ttgagaattc aaccgtttga
     5161 gattttgcaa tcaatttggt acgattttgg acatccatat ttgcagtgac cgcagcaata
     5221 tgtcttgcac cagggcgaat gaaataattc acatacctaa tcagtgcatc tggctccggt
     5281 accgtgtctg catcattgct taaaatcaat ttcccttttg caaaggcaag tccgatatta
     5341 aacgcgtggg cttttccttt atttttttcg atccgaacca cacgaagatt agcgtatttt
     5401 ttcatcagtc tagctaatat ttctggtgtt tggtccgttg aaccatcgtc tgtcactaat
     5461 acctcatagt tgtgataatt caatttcgtc atcaaatact cgattgtata ttcgatcaca
     5521 atttcttcat tatgggctgg caccatgatc gtgatgaacg gttcgacact caagggaaca
     5581 tctacccatt ctttttgctt atgtttgtat aaaaacacat aacaccagac accaataaac
     5641 catacaaatc cgcctaaaat cggataagaa acgagaaaaa aatacataat agacaaacaa
     5701 gtatctaaga ataaattacc tgtatgggtg attgagatca tttcagttcc accccatttt
     5761 ctttatacaa atctgcaatg aatgtcgtat ctagattttt ctcttcggag acagaataaa
     5821 aacggaccgt ttctctctct tcttttgttc caaaacgttc cgtatacact tcttcaagta
     5881 attcttgtct ttttttcaat ttttcatctt catattcaaa agatttattt acttttttgt
     5941 atagatttcg attgttccac ctagtcaata caaatgaggt aatcaggaaa ctgaaaaaaa
     6001 tcaatacaaa aaagccgatt aagaaataca gcattttctt ttctgataaa aaagcgtaca
     6061 aggaaaaatc cttggctctt tctggtctcg ttaatgggaa aaagacccat ataaaaggaa
     6121 gaacaacgat catccatcct aaaagagcca ctaacgtttg ccaaatcttt aaccagtaat
     6181 taccttttga aaaatactta tctttcacca aaattgatgc aacttcttct cttgttactt
     6241 ttttcatatc ctcacctcac gtttttttgt tacgacgtag gagtttacca tacatttcat
     6301 ttcttttcac ataaagtgtt cctaaatttt tcctaagaaa aagaacaaaa catttctcat
     6361 cagcaaatga aaaatatttt gttcttttta tcagttgttc tttttatttt ttatctctta
     6421 attatgttgc tctttccaat acgacaatgc ttttcattcc tctagatatc gccgtgtaca
     6481 tttgtctgcg tcttctttgc tcatctccat cgttttcaaa tggatataag attacatgat
     6541 caaattccag tccttttgct agactcaaag gaatgatttg gcagttttct aatccatatg
     6601 aatttttttt aatcgattct tgtagcgttt gggtttcctc ttctgtttgg gtgataactg
     6661 ccgctttttc tccttttaga gaaaccaaaa tctcagctag tattttcaac aattcttcag
     6721 aggatcgaaa agagcggtat tcgggttctt ttccttgcgg tcggatcgat acgatgtcga
     6781 tcttctcttg atccacagca taggttttga aaagctctgt gattgctccg ctagaacggt
     6841 aactatttct aagatcatac cgatggatcg gcagatggta gtcgtcgaaa caacgcataa
     6901 tatcagcaaa agtagtggaa gaattaaaga ttgcctgatt ctcgtcacct actaaagtaa
     6961 agtgagtttt tggaaataat tcgatcaata gacctaattg tgccagggta taatcttgta
     7021 cttcatcaat caatagatag cgaaatgctg gtacatttac cttttcaaca agtaaatgac
     7081 gaatagctaa tataatgact gcttcatcaa cagtacgtgt gggttgccaa gcataagctg
     7141 agccatatcg tttttcataa attctttcaa acaactgatg ctcatccacc catgccattt
     7201 cttcgatttg tctggttatt tttcgatatt ttttttgtaa aagtttcctt gcataagcta
     7261 caatactttg ttccgaatca tcagaaatca atttaccgaa taattccaac tgcctatctt
     7321 ctgtcaacga gagtacctga ttctgcacag caggactttt tgcttgtttc aaaagatgat
     7381 tcttccattc gctcatcagt gcctttttcg ttgcttgaag cctatcgatc aatttaggat
     7441 gagcaggaac tttctcatat attttttcga tcgttttttt agaaaggact gtttttccat
     7501 ttttagtaag tccacggata aaattcggat gatctaaaaa caaaggatct gattgtttta
     7561 gattatcgat aaactttttg ctacgcaaac gttctgtctg ctccgataca ttttctcccg
     7621 tgatacgttt gaaataagcc tcttcacctt ctatttcctc tgctgaaagc tgtgaaacca
     7681 gctgtatgat ggttagattc agtggatttc tttcacctaa tgatggtagg acattcgaaa
     7741 tatagtcgat aaaccgatga ttaggtgata gaatcagcaa gtcatcggat gtcatcgtat
     7801 ctcgatataa atagagcaga tacgcaatac gctgcatgat agcagaggtt ttaccacttc
     7861 ctgctacgcc atttaccaag atattttgac ttttcacatc ccgaatgatt gtgttttgtt
     7921 ccgattggat cgtagcagtg atagccttca tttcattggt ctcatcttgg cctaagactt
     7981 ctaataatac gtcatcttga atagctacag acgtatcaaa aaacttgacc aaacgatctc
     8041 tttccaaaag aaattgtcgt ctttctttaa taaccgtatc gattttattt tgacgaaccg
     8101 tataagaaga tggaccaatc atgttattgt aaaaaagttc ggcaattggt gaacgccaat
     8161 cataaacaag attttcatct gcatcatctg tgaaatttgc tgtaccgata taaaaggatt
     8221 cttttggctc attatccaga aagtctacaa cgacttttcc aaagtaggga cttttcaata
     8281 aacgttcgat ctttgctagc tgattttctg cgtttttctg tttgatattc atctgatcga
     8341 tttctctatt tttcatttca atcatcgaaa aagtatccag gttatctaag taactgtcaa
     8401 aattcaatcg aagctcgcca gccatttctt tcaacgactg gtttccttct atttttgccg
     8461 tatccatcga ttcccgcagt tcgttttttt tatcaattag cttttgatac acgttcttca
     8521 gatatgcttc ttctaactgt ttttgtgtgt tgaccataca cattctcctc tttttttaaa
     8581 gtggtggaac cgattagttt acctctttta agagtcgaat gcaagctttt taacgagtat
     8641 catttgttag gatatatcag ttcttcaggt cggacagttt gatataaaga aaatgcacca
     8701 tcaaggtttt ttacgtggta gccagactgc ttcaagattc tttccgctag atagctacgt
     8761 aatccgctat gacagctgac gatatattct tgttggctgt ctaattcatt taatcggtcc
     8821 cgaagttcat tcaaagggat atgtttggct tccttgaatt tgcccttttc caattcttct
     8881 gctgttcgca catctaataa aatttttcct tttgctagtt ctgtcggcaa ttcatgccat
     8941 tgtacggttt cactaagccc ttctgcaatg ttcattgctg catatcctag catgtttact
     9001 ggatctttgg ctgagccaaa tggcggtgca tacgtcaatt ctaattccgg taaatcgaag
     9061 atcgtcaaat gccctttgat tgctgttgct aaaatatcga tccgtttatc cacacctttt
     9121 gccccaacac cttgtgcacc ataaatctcg ccagtctctg gatggaagat caacttcagt
     9181 aaaatatctg ttgcttctgg ataatagctt gcatgatctt ttcctgatac atgaatgact
     9241 tcaaaagaca gttcgttttc tttggctgtt ctttcactta aaccggtgga tgcggcagtc
     9301 attccaaacg ttcgtataat tgctgttcca ataccgcctt gatttcttcg agaaattccg
     9361 gaaatcgtgt ccgcaacttg tctaccttga cgattggctg gtgaagcaag agaaataagt
     9421 gcatcttttc cagtgatttg ctgttttaca acgatagcat cccccaccgc ataaatatca
     9481 ggaatattcg tttgataacg ttcgtctact aggatacccc cgcgtatgcc taattcgatt
     9541 ccagcggtca ctgctagctg gttatcagga cgaacaccaa tggataaaat aactaaatca
     9601 gcagggatgg tacgcccatc atccagtcgc aatcgtttcc cgtggtcttc aaatccagcg
     9661 acagcatgag aagtaatcac ttctactcct tgatgagaca attctttttc tataaaagct
     9721 gccatttctt catccagtgt tggaagaata tgtggtgctt gttcgatcac catgacttct
     9781 aaacctcttc tttttaggtt ttctgccatt tccaatccga tgaatcctgc accaacgatc
     9841 acggcacgct ttggctgttt ttctaatgca tgcatcactt tatcgatatc tggtacattt
     9901 ctaatagaaa aaacattgtc ggcttctgtt atccctggaa tcgatggaac aactggtttt
     9961 gcaccaggag ataaaaccaa tgcatcgtac tgttcaatgt gtttttgacc ctcatgtacg
    10021 acttctaccg ttttgttttc gggaaagatg gctgtcactt catgatttgg acgcacatct
    10081 aaacaaaaac gagcggataa tgattctggc gtttggacaa gaagattttc ccgttcactg
    10141 atttctcccg aaagataata aggcaagccg caatttgcaa aagaaacata cggtcctttt
    10201 tcaaaaacaa cgatttctgc atcttccatt aatcggcgaa gccgtgtagc agcagacatt
    10261 ccacctgcta cacctccgac aataacgatt ttcatcttat ttccctccta ctgttcgatc
    10321 aaaccattga ttcatgccac cacgtacatt cactacatca taaccagatt tctttaattc
    10381 tttagctgct tgtttactcc tcatccctga ttgacaaatc acatagacgg tcttttcttt
    10441 gtctccatca tatctgttta tactttgtaa tggaacattt ttagctcctt ttatatgccc
    10501 gccccggtat tctgcaggtg ttctaacatc aagtaacgct ggattttcca aaagcttttc
    10561 ctttaattct gggactgtta tcgattttat tgattgaaat agcaaattca ttttctgcac
    10621 ctcatttaca tttttataaa tacctcatgg ggtatattaa acaggagctt tattaatatg
    10681 tcaaccagaa tgactcttca tttttttcac tactttttat atttgcataa aataccgata
    10741 aggggtatta tctaattata aaaatagagg agaatgtgtt atggagattg ataataaaaa
    10801 tatcctgaac cgtttgaaac gtacagaggg acaaattcgt ggtatccaaa aaatgatcga
    10861 agacgaaaaa gaatgtatgg atgtcatcac tcaattaagc gctgttcgtt ctagtattga
    10921 tcgcgtaatg ggcatgatcg ttgcagataa tctgaaaaat tgctttgaga aacctgatag
    10981 taatccagaa gaacaagcgg ctaaactaga acaagcgatc aagatgatta ttaaaaagta
    11041 aaggaatgat ttcaatgaaa gtcgttttcc atattgatga attagaaaaa tggtcagaaa
    11101 cagggaaaaa cgtaaaaaac ctgataaaag cttctccaga aacgacgatt gtcgtatctg
    11161 taaatggcat cgcgattacc ggttatctag actctgccaa tgcagaattc ttagatttgc
    11221 aaggagtcgt ttttcacgct tgcgccaatg ctatgcgtgc taatcatatt tcagaaagtt
    11281 ctttacctga acaagtaatc gttgtaccag caggtgtcct tgatttagtt gaactgcaat
    11341 cccaaggtta tgcttatatc aagccttagt cagtagaggt tgcgacagaa gtgatcagct
    11401 tcaagaaatt tcggcttatt tttcgaagaa gctacttctg gaaaaccgtg aatcaaaggt
    11461 tgtgaaaacg ctgtcctgca tcaagtaata agaacagcca aacaaaaata gcttctcata
    11521 tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttaaggaag
    11581 aggttgtgaa atcagcggtt tgacctaagt attagagaaa aaaacgggaa aatagctcct
    11641 catattttgc cgttttttga gctaataccg aaggtcagct gattgaacac cgtttaagga
    11701 agagattgtg aaaaagtcgc ttagtttcga gtattaagaa aaattttgtg aaaatggatt
    11761 tctacatttt tgcataaatt tggcttaata ccgagaaact ggcttttgaa taccgtttaa
    11821 ggaagaggtt gtaaaatcag cggtttgacc tgagtattag agaaacaaac ggaaaaatag
    11881 ctgctcatat tttgccgttt tttgagctaa taccgaaggt cagctgattg aacaccgttt
    11941 accaagaggt tgtgagccta ccatttaact ccgaatcaca agaaataatt tcaaaaacaa
    12001 cttctcctgt ttttggttcg gttggcttat tatcgagaag tagctactgg gagtctattt
    12061 atcaaaaaaa ggaatgcgac ataggtcaca cccctttttt attttctttg aaacatcttt
    12121 cggtactcga cagcatacaa gacaaaagat tgtaaaagac cgtaagtgat gacggcaaaa
    12181 atgactctca cccacagcgt tggtaattct gggaaaagca gcaaaaggat catgccaaca
    12241 tagatgacag cagtcgctac tttgccatac cacttcgctc cacttaattt gatattttta
    12301 gatagcccga ttaatccagc tacaaacata aagccttctt ttatcaagaa tagaccaaac
    12361 aacagccaaa gccacggtcg ataccatgcc atagacaaaa tcagaaaaag ttgtgtcaat
    12421 ttatctgcaa acggatcaag caccttccct aagtctgtta cctgattata tctccgagca
    12481 atgaccccgt ctagataatc agacaaacct gagacaacaa tgaccgctcc tgctatataa
    12541 aatgactgtc tcagcacaaa ataaagataa atcggcagca aaaaaatacg gaatatcgat
    12601 aaaagattcg gaatcgtttg tatttcttta cgccagtctg tgcttaattt cattttatcg
    12661 ccctacctcc tcagcactct tttcttttta ttttagctta cacggggaat aaaaccaatg
    12721 atttcactgt ttttattttt ctttacagac actgatatta tgagctaatt tgagataatc
    12781 acttgctgtt ttcatcgttt actcttcttt cttcctgtcg tttgcgcatt tcttttacta
    12841 gtactttatg agcttttttt gatcctcgct ctaggtcacg ctgccgttta cgtgttttat
    12901 aatcgataaa cagactgtct aaatcgtaac ctgtcgggta cagatcttct gctgaacgca
    12961 ccagacgaac acgctgacga ggcacgtgga ttattttttt gtctaaatat acatcaatcg
    13021 tatcttcacc tatatcttca taaactaacg cttctttttg gtattcattg acaaatatcc
    13081 gatcaccttt tgcaaagagc tgacgttctt ttttattctc cttcctattt gtaactgctg
    13141 tcttaggaag aaattctttt ttctgtgtcc gataggcttt ttccgccaag taacgctctg
    13201 cttgatggat caatttcatc gacatatcca ttttatgagc aatccacaaa gcttggcttt
    13261 ctcctgtttc ttccaacccg caactgatat ttaggacgaa gtgcttcccg atcgaaagcc
    13321 atagcagccg gcacaaaatc ttcatgatct ccggcgaatt tcttgatttc tccataatgg
    13381 gtcgtggcaa tgatcaatgc cccttgctca tacatagatt ccatgattgc gatcgctagt
    13441 gcagcccctt catttggctc tgttccactg ccaatctcat ctaaaagtac caacgtattc
    13501 ctttttatct ttttcaaaat agcagcgatg ttttgcatat gaccagaaaa tgtactcaac
    13561 gcattttcta aattttggtg gtcaccgaca tcgacgaaaa tctcatcaaa gacagcaagc
    13621 tctgtccctt cttttgccgg aacttgcata ccgaacatcg ccatcaaagt caataaaccg
    13681 actgtcttca acacgactgt tttgcctccg gcatttgctc cggtgataat caagcctcga
    13741 tagtctttac cgatttcaac atccaatggt accgcatgct caggtaaaaa tggatgtctg
    13801 ccttgcttga taatgatatg ttccgattta ttgattctag gtgtgcttcc attgatttct
    13861 cggctaaatt ttgcccgagc aaagatgata tctaacaccg tgatcgtttc gatcaataaa
    13921 tcgatcactt cttcgttttc tgcgatagca ccagtcaatg ccgccaggac ttgatattct
    13981 tcactgattt cttcagcttt taacaactga tagtgttcat tcaatttaga aatcacagtt
    14041 ggttcaataa aaaccgtcgt tcttttattt gattcatcga taatcgttcc atctatttta
    14101 tttttatagc tggctttgat aggaatcgta tagtattcgc ctttttgaac aatcattgct
    14161 tcttggatca tttctttgtt tttcggatga cgcaagaatt tcagtaattt tgactggatt
    14221 tctttttcga ttagttgtaa ttgtttgcgt actttacgca aattacgtga cgcgtcgtca
    14281 ctgactttat tattttttat tttctggtca atcaactctt ctacatttat caaatcagga
    14341 agatgtttgc tataagcaaa caacaagggt gtctggtatt gattcttatc aaaaaacttt
    14401 gtgatcatcc gactgcttct aagaaaatcc gcatattcga tcaaatcggt tggctgcaga
    14461 atcaaccctt ttttgacttg ttctgtcagt gcatcgatcc tcggcaaccc cataaatgga
    14521 acatgctgac tgctttctaa gatcaagcgt gcttctcttg tttcttcttg acgatccaag
    14581 acagtttgta ggtttgtcga gaccgtcata tcgctgattc gttttttgct atagtttcca
    14641 attgctcttg cttggatttc ctttttgatc cgatcaaatt gtaattgact aatcatttcg
    14701 ttgtccatat tctctccttt tcttgaagag atacctgtcc caaaataaat aagggcctag
    14761 agataaataa aaggatagaa cacagcttaa aaaagtcatg tcctatcctt attagaatac
    14821 aaagatattg ttttcctgac aggtaccgtt tcataagcac accaaataaa ctttgtttat
    14881 tggctattcg acttactgat tagataccag ctacactcac aaaacatacc ccattcttct
    14941 aggtcttatt tattttccac ttaactatag caagacacat tttggttgtc aaactttagt
    15001 atgttttata gatttacacc attaatgaat agacacctac cgcgataatt gctccgatca
    15061 ttggacctgc gattggtacc catgaatatg cccaatctga agttccttta ttagctattg
    15121 gcaataccgc atgtgctaat cgaggaccaa agtcccgagc agggttgatt gcatatcctg
    15181 ttggccctcc taatgaaaga ccgatggcta agatcaatcc acctacagcg aatacgttcg
    15241 ttccaggtgc aaattcgttt tgtccaaaag ctagcaaccc aagaactaat acaaatgtac
    15301 caattaattc tgtgatcaca tttgctggat aattccgtat agcaggacct gttgcaaacg
    15361 ttcctaaaat cgcgctttca tctttcgttg cattccaatg tggcaaataa gaaagccaaa
    15421 caagaatggc ccctgcaaaa gctcctaaaa cttgcgcaat gatgaaagga acgacaagat
    15481 tccaactaat tgcccctgtc atcgccatag ctagagagac tgcaggattc aaatgagctg
    15541 ggcccatgaa tccagaaata tagacagcca ttgttacagc gagtccccaa ccaaatgcga
    15601 tgacaatcca gccagaagcc tgtgctttac ttttgtttaa attcacagcc gcacacacac
    15661 catctccaag caaaactagg atcagtgtcc caataaactc ccccatgatt tgagtcatat
    15721 cactgttcat ttatttctcc ccctttagtt cccgcagatc tgattctgca atcaatgctt
    15781 ctaaacgttt agtctgttgc gccttctcct cttctgtcca ttcaaaatat ccagcaattg
    15841 catcgatcac aggttgcttg atctcatcaa gctgatctcg ttcaaacaac agatgatttg
    15901 tacgacgaat gagataatcg cctggggcca aaaccatttc ttcttctaaa ccataacgta
    15961 accgagctga ctcagcgaga ctcaaaccag gataaggtgc catttctttt gctaattcaa
    16021 agatacgtcg ggcattagtc ccgtaaaaat cagcgatata agtagcatct tcttcctcta
    16081 aaccggctgc tactccttct tttgctaatt ctgtcactgt ttcttctaat ttagttggat
    16141 cgaagtttcc acctgaaatc tgatattttt tagagtcgat ttctttcgtc tctattccgt
    16201 attcttcttt taataattgg cgtatcaatc gcaaagctcc ttctgccatc ttacggtaat
    16261 cggtgatttt cccaccagat aaagtcagca aaccatctgg ttctctttct aaagaactac
    16321 ctctggaaat cgtagaaggt gctttttcat cacggctgtt ttccaaatgg ttcaacacgt
    16381 cttctacttc agcacgagaa actttgtttt ccttatattc acttactgta tcgacaactt
    16441 tattgaagct cttgtctgaa atcgatccat tatctccacc attgtaatca gaaccagaat
    16501 taccaatcaa cagtggacga agtcctgccc agcttgcttc gatatcattc aatgtgatat
    16561 ttgcttctgg atagcgatga ttgatcacat ccaatagata atctacatct tcttgtgtga
    16621 ctttcggatc cgtaaaatct ccttggtaat ccgtatctgt cgtaccaaag taagtcttgt
    16681 tttctcttgg aatagcaaaa accatccgcc catcttgctt tcctgtatcg aagtatgtgg
    16741 gttgcggtac aggcagtttt ttcgcatcta caactaaatg gatcccttta gttggacgca
    16801 ttttaggaga gactggacgc gtaaaattca agtttcgtac tttatctacc caaggaccac
    16861 tcgtattgat cactaatttt gctttgattt cgatcacttc atctgtcagc aggtcacgag
    16921 ctttgacgcc aacgatttga tcgccatcat ataaaaagcc aaccgctttc attttactta
    16981 ctagataagc cccatcttcg gcagcctttt tgatattatc aatcactaaa cgggcatcat
    17041 tgttgcggaa atctagatag acacccgcgc cttttagccc ttcttttttc aaaaacggtt
    17101 ctctttccaa tacttcttct ggtgtcagcg tataattctc atatttggtt cctgtcacat
    17161 ttgccagttt gtcataaagg tccattgcta ctttgaccga gaacatattg aaggttgttg
    17221 ctccttcgcc ctcatagatt ggcaaaagca ttggatctgg ttttgggatg tgtggggcaa
    17281 tactttgtac gactgcacgt tcaccaactg tgtcagctac tacttccaca tcaaatgtct
    17341 tcagataacg aataccgcca tgaacaagtt tggtcgagcg agaggaagtc ccttctgcaa
    17401 aatcctgcat ttcgatcaat cctgttttga ttcctgatgc tgctgactgg actgccacac
    17461 cggcaccagt gattccgcca ccgatgatca acagatccaa ttcttcttgc tgcatttttt
    17521 gaatgctatc ttgtcttgtc ttgtttgaaa acatatggat ttcctcctta ttcaaatgct
    17581 tgggtagctt ttactgcttt tttccaaccg ttgtaaagtt tttctcggcg ctcttcctcc
    17641 atttgaactt caaatagctt gccagcttca taaaattcgc ggatctcgtc tgtatctttc
    17701 caataaccga cagctaatcc tgctaaaaat gctgccccta gcgccgttgt ttccaagttt
    17761 tctgctcgtt ggatcggtac attcaagata tctgtttgga attgcatcag atattcattg
    17821 tttgctgcac cgccatctac tttcaataca ggaatcttga ttccagtgtc atcttgcatc
    17881 gtatcgatga tatctctcac ttgataagca atcgactgta aagtagcttt aatgatatct
    17941 tcttttgtcg taccacgtgt caagccaaac atagcccctc gtgctgcctg atcccaataa
    18001 ggagcaccta gcccaacgaa tgcaggaaca acataaacct catcttcgct tgtagagcgt
    18061 ttcgctgcgt cttctgaatc gctagctttt tgcaacattt gaagtccatc tctcagccat
    18121 tgaatcgaag aaccagccac aaaaatactt ccttctaaag catagtaaac ctttccattt
    18181 atgccgtaac caattgtcgt caataaatta tttttggata attgcggttt ttcaccggta
    18241 ttcatgacaa taaatgaacc tgtaccataa gtattcttga ccatgcctgg ttcgaatgcc
    18301 atttgtccga ataatgcggc ttgctggtct cctgccatcc ctgaaattgg tgcttcgctt
    18361 ccataaaaat gataaccttg cgtataacca taaacttctg agttagaagt gacttttggt
    18421 aacatcactc gaggaatatt tagtaaatct aaaatctcct gatcccaatc tagctgatgg
    18481 atattgaaca acatcgtccg actggcattc gagtaatcag taacatgtgc ttgtccatcg
    18541 gtcagtttcc atactaacca agaatcgatc gttccaaaca tcaattcgcc tttttcagct
    18601 cgttcttgcg cgccttctac atgatcaaga atccagcgta tcttcgttgc tgagaaatag
    18661 gcatcgatca ctaaccctgt tttttcgtgg aaaaattctt gatagccttc attttttaag
    18721 ttatctgcaa ttccagctga ctgtctggat tgccaaacaa ctgcatgata gattggacgt
    18781 cctgtatctt tttcccatat tactgttgtt tcacgctggt tcgttatgcc aatcccagca
    18841 atctgatttg gtttgatccc tgattcgatg aacgctccgg caataactga ctgtacagaa
    18901 ttccagattt catttgcatc atgttccacc caactttctt gcgggaaata ttgcgtgaac
    18961 tctttttgag aactgccgat gtgtcttcct ttcttgtcaa agataattgc tcgtgagcta
    19021 gttgtacctt ggtcaatcgc catgatatac gttgattctg tcattgtgat tccctccatt
    19081 atatgcaaac gctttcttta actactctta tcatactact gttttaaaaa aagaaactgt
    19141 caaaatttta ttcttttttt ctttttttga cagtttttta ttatgaagct attttttaat
    19201 caattctttt cttttttcag cgtctctatt cttcttttca atcgaataat gtcgtaaggt
    19261 gacgcaaatt ctgacagata atattcacac tgttttttgt ttctctggca aaatgtgatc
    19321 accaaatcat attctttacc ttcttcataa cttccaatct caataccgcc gattcccctc
    19381 agttctctga tcagatattg ttgaaaagca atacgataaa tcggcagact ttctaaatcg
    19441 attccaatca ctactggttt tgcaatataa aaatcgatca tcaaaagaac attggcatac
    19501 tggatcatca ggtaactaag ctcactatct tcagctcttt tcctcggcaa ttgctgcacc
    19561 gctgtttcct gtaatttcgc cagtaactgt gttaaggaat gcccgagcat tttctgctgc
    19621 tgttccaaaa gccgatcacg ctcatagact tcgatctttc caacaaagaa aaaccattca
    19681 ttgttgatct gggaaatttg gtagcctact gccttttctt ctttgatttt caaacgacga
    19741 ggacgataat gccataacat cgtttcccga atgtaagtat ctaaaacagc ggttggtaac
    19801 tttttgctcc gtgtcaaatc atactgataa aatatttctt cgtctactat cgaaaaagaa
    19861 acaaaaaagc tgtaaaacat cattgtttcg tatacattca gttctgctgc tgttcgacta
    19921 agatgcatca cgatgattgg atcgattgct tgataaagcc ggtccgactg atagagtaat
    19981 ttcttttctt tcaaagttgc gtacgtactt ttttcattca atagtctttt tttcgtaatc
    20041 ccgagccagc aagcaatctg tgcttctgct gatgccgtaa acgttgtttc taatacacgg
    20101 tccaatccta gaatgaacgg tcttttttct ggtgtatttt gcaaaaaaag cggtcgctga
    20161 tcttccggga tatattggaa tagttcataa taaaaatatc gtatctgtag ctcttctccg
    20221 tataattgac cgtttttgat ttgtaatcca aattcctcta agagctggtt caattctttg
    20281 atctttcgaa aaacagaaga ctcactgata ttaaacgctg tcgctaatcg aacgatggat
    20341 acttgctgat gttctaataa atagacgagc atcttaaact tcaagctttc ctgcaataaa
    20401 aacaatagaa ttttttccaa acttacatct tcgtccaaca caagcagcgc ctgatactca
    20461 tttcgctgta cttctacttt tttttccatc attcggccaa tcaattcaat atcttccaaa
    20521 tattgatcaa acgaggcttt tgacagttgg acatgctctc tcaaaccatt gatagaggct
    20581 gttccccctt gcagaacaag gtttcgcaaa atcaggatct gtaactgctc ttttctttct
    20641 aataatgctt caatcttcat aggcagtatt cgccaccttt gaccacacca tatctggcac
    20701 acggtattct ggctggaaaa atagctcatg ttcttctaac tgttcaaacg tccatccttc
    20761 ttctactgca attgtcagtt ggtcaagaag gtggaacatc tcttttttgc tttggagctg
    20821 tgctccaaga atcttttttt cagaatcaaa tagaaatttg agtttaactt tgctttgatt
    20881 tattgctgta agcttgtagt ctacgtatcc tgtttcaatt ttttggggat aaaaaatgct
    20941 ctcttcttct gtcaaaccta cgcttcctaa gaaatagcca aaccactgat tgccgactgg
    21001 acgataggtt ccctgatcct ttgtctgtga gccactaatt gtcttgctga catttcgtgc
    21061 tgtacggaca gcgttattta ctagagaaac atacattttt tcatttgttg gacgaaaagt
    21121 tacttggatc gcatcgccaa tcgcataaac gtctttttga gacgtttgca agtagtcatc
    21181 tacccagatc gttccatcat cattcaatgt taattgttct tcccacatct gattgtcggg
    21241 acgagaatga ttagccagca aaacaagatc atagttctcc tttctttttt ccgtctctac
    21301 aatagcggtt tcattttcgg tcaaagacac aacttgttct tctaaaaaaa gtgaaagccc
    21361 ttgatttttc atttccttta ctaacggttc tgtcatttca ggatctagat aacgaaaaag
    21421 aattgacttt cggctttcgt aaagatggat ttgttttttt cttgatgcca acccttcagc
    21481 tatttcgatt cctgcttgtc ctgcaccaat aattgcaatt ttctttgcat tcatgatctt
    21541 ttgctgaaga gccgttacgt tttgtatctt tttaactggg tgaataagcg ttgattctgc
    21601 agaaatattt cgaaactttt gatttgaacc agtcgctaga atcaaacgat caaaatccaa
    21661 tttttttcct cctgctaaaa cgactttttt ttctttcaat cctattactg tcttttctgt
    21721 gtaaacatgg attcgatagc ggtcagtcag ttcttctttc gtgatccaag aaagttgttt
    21781 ttcactggta acctttcctt ttaatatcaa attgaacccg ctgggaatcc atcctaactt
    21841 cgattgcttt tcaatcaaat atatttcact tgttggcatc aattggcgaa gataaatagc
    21901 cgcatgaatc cctgcaaacg acccgccgac aattacaatg ttcatctgca ttcctctttc
    21961 ttataaggtg ttctactgct aacttctcaa tcagaaaaca cagtattcat aactcgcttt
    22021 caaccttatg atacatagcg atgcagcaga atcaaccctc tccatcatac cgtgttctag
    22081 gtctgcttct caataatgta ctagtggacc taattgaaga gtatcatttc tgaagagatg
    22141 tttcaaaaaa tgtactctta taaaagaaaa aaactcgcct tttacgtacc tctgtccaaa
    22201 taggaaacag aaaagaacga aaagacgagt tttagcgtgt gtaatgtaat agttagaata
    22261 gacacatcat ttatttttaa ttctttaatg cttcaacaac tgcttcgcct aattgatgag
    22321 cagattgagg attttgccct gtaagaagcc gcccatcaat ttgaaccact tctgaaaaag
    22381 ctgctccaga ctgatagttt gctccttttt ctttcaaagc atcttctgct aagaatggta
    22441 cttcattcgt tgtaccattt gcttcctctt cttcattact gaacccagtc actgtacgtc
    22501 cagctaaaat agattttcca gactcatctg taactggcaa caatcctaca acaccgtgac
    22561 aaacagctga gaccagccct tcatttcgat agatttgtga agcgactttc ccaagatcta
    22621 ttgcttctgg aaaatcccac attgttccgt ggccaccagc ataataaacc gctgcataat
    22681 catttgcatc cacatcatca ggacgtaagg tatttcccaa ggcccgattt ctgaaatctt
    22741 catcttcata ataggcccaa tcgatttcat ccatattcaa aacactttgc ggttctaaag
    22801 gtacatatcc gcctttagga ctgacaaaat ctgcatcgat cccatttttt actaatacat
    22861 cataaaaatg agtcagttca cttaaccaca aacctgtagg acgctcgatc ttttcgtact
    22921 tttctacatt tgttaatacg atcaatactt tcattttccc atcctctttc tataaaaatt
    22981 gcgttattga taacgtgctt tcaagaaatt atataatggg cttggcaaat aaagctcttt
    23041 atatgcttct tcaaaaggtt tcgttacaaa ttcatcctca cggttttcca ataatttctc
    23101 tgcccagtca ggatcgacga tcatttgctg tccaattgct gcaagttctg catatttcaa
    23161 tacctgctct acatcttcgc gattgcggat cccaccgaca ccaactaaag ggatctttcc
    23221 ttgaatcgtg ttatggacca cttcaagaat cgatttatcg ttgtatttat cagaacgagc
    23281 tattcgatca tacatattta atgaaacatg cagataatct agttttgact cacgtaatcg
    23341 ttcaagaagc cagatcgtgt cttcaaaacg gatacctggt gtttcatatt cttctggtga
    23401 gaaacgatac cctacgatga atggtttttt agcatattgt tcaaccgctg agaaaaccgt
    23461 tgttaataat tcttcaataa aacgataacg attttccaag ctaccgcccc attgatcctc
    23521 tcgtctattc gaatgcggag agaaaaattg ttggatcaaa tacgtatttg ctccatgcaa
    23581 ctcgattcca tcaaatcctg attcaatcgc tctacgtgtg gctttgccaa atgcttggat
    23641 tgtttcaacg atttcttctt cactcattgc tcgtggtgtt tccgcatcag gacgttttgc
    23701 tgcaactgca cttgcagata caggctgttc tccagataaa gttgctcgac ttgtcattcg
    23761 tccaccgtgg aaaatctgaa taatcgcttt tgcgccttct ccttggattg ctttcgctag
    23821 ctcacttaat cgaggaatca tttcatcatg agcgatgctg agttcacctg gccagccttt
    23881 tccgatatct tggacatttg cagcaccagt aataacagca ccaagacctt tagaacgttg
    23941 tttataataa gtgatctcat cttcggtgat cactccattg aaaaagctga gttgtgtcgt
    24001 cataggcgac agcatcaatc ggttcttcaa ttgaactcct gaaggtaaag taattgattg
    24061 atcaaattgt gacattgtca ttcctcccat aattcgctct gattaaccta tcgagaatca
    24121 tctatcatta aaccactcga ttagttgtac atgcaactaa tttgcttaaa aaattttatt
    24181 ttttttgtaa cttatctgtt gcatcattca ttgtctgggc taattcttta cttgcttcat
    24241 ttcccaaaac tgctttgtat gactgatgaa gttcttccca gctaccatcg acttccttag
    24301 caaattggcg tccttgttct gtaagataaa ctaatgacaa gcgaccttcg ctaactgttt
    24361 tgacgtatcc tttatattct aatttttcaa caaaacgagc aatcgttgat ggggcggaat
    24421 agagcatttg tgcaatatct ttttgtggta ttccatcttt ttcttttaat atcaataaaa
    24481 taaaaccata agttggtgcc agacctgtcc ttctaaacgc ttcatcagct aatttattca
    24541 acatgcgatc tagctttttc acgctaaaga aaagacattc atctaacgct ttttcaattg
    24601 gcttttccat ttcatcccct ccattggcgt acatatcata gtatgatttt tattttttgt
    24661 caaaaaacac ggaataatgt ccgcctcctc tgtctagatt tgaaaaaaag aagtagccat
    24721 cttcaaagaa tttcggcttg ttttcgacaa aactccttct ggaacaccgt gaatcagagg
    24781 ttgtgaaaaa gtcgtttagt tttgagtatg aagaaaaatt ttgtgaaaat ggctttccac
    24841 atttttgcat aaatttggct taataccgaa aaactggctt ttgaacaccg tttatatcag
    24901 aggttgtgaa aaagccgtcc tgcatcaagt aataagaaca gccaaacaaa aatagttcct
    24961 catattttgt cgttttttga gctaatgccg atggtcagct gattgtacac cgtttattga
    25021 gaggtcgtga gcctgccgtt gaactccgag tcacaaggag caatttcaaa aaacagcttc
    25081 tcatgtttta tgactatggt gacttgtgat cgaggagttg gcaggagaat actgtttatt
    25141 cgtaaagaag gggctgtgac aagacttttg tcacagtccc ttatttcctt ttattatagg
    25201 atttcgccat ttgattggat gacttgtctg taccaatcaa atgatttttt ctttaaccgt
    25261 ttctttgtgc cattcccttc gtcatcttga tcaacataga tatatccata acgttttgac
    25321 atttgtgaag tagaagcgct gacgatatca atgcagcccc acgacgtata accaaataaa
    25381 tctactccat ctttgatagc ttccttcatc tgctcaatat gctgcttcaa atagtcaatg
    25441 cggtaatcgt cttgaacggt tccatcttct tctactttgt ctatagctcc gagtccgttt
    25501 tcagctatga acagtggttt ttgataccta tcatataatt gattcaaagc aattctcaaa
    25561 cccttaggat caatctgcca gccccattca cttgatggaa gatacggatt tttaattcct
    25621 ccagttatta agtttccttg gaccccttcc caattttctg gatgagcact gactgcacta
    25681 gacatgtagt agctaaaaga aacaaaatca acagggtagg ctgctaagat ttcttcatca
    25741 cctacttcta tttttaagtt gacacctttt tcttcaaaca ttcgttgcgt atacgctgga
    25801 taatatcctc ttgcttgaac atcactaaag aacaaagaag ttctttgctg ctcaattgtt
    25861 ttttgaatat catccggatc acaagaatac ggaacaagaa gctgatagct gatcatacaa
    25921 ccgacttgtg aacctttgat aatttcatga caagcttttg tagctaaagc acttgctata
    25981 aactgatggt gtgcaccttg gtatttcgct tgttccaaat ggggatggtt ttcttcaatt
    26041 actcctacgc taacaaaagg gtgatgtttt acacagttga tctcattgaa agtcatccaa
    26101 tattttactt tatttttata gcgtttgaat actgtttcag cgaaacgaac gtaaaactga
    26161 ataagctttc gattaggcca tccaccatat tctgtaacca aatataacgg catttcataa
    26221 tgggaaagag aaacgactgg ctcaatccca tattttagac attcgtcaaa cacttgatca
    26281 taaaaaaata atcccgcttc attgggtgtt tcttcatcac catgaggaaa aattcttgtc
    26341 catgcgatag acatccgata gcatttaaaa cccatttcag caaataaacg gatgtcttct
    26401 ttaaagtgat gatagaaatc atttccgtgt cttttaggat aataatactt gctgttggga
    26461 tcctgcgccg cttttacttg ctgatgcgtc atcatatgcc attgatctgt ccatttttct
    26521 ttaggtaaat cgctgtcgaa tgtatacaca tcagacacag ataatccttt ttcatctaca
    26581 tcccatgcac cttcacattg attggccgct gtcgctccgc cccataaaaa tccttctgga
    26641 aattttttca tatcgatccc cctaaacttg ctagttttct tgtttgatta tttttcttat
    26701 gactagtaat gatatttttg aatctactac tatttgtaac aataaccatt gtggtcggat
    26761 catatccctc tttgattaat tcttgcaaat ctacttccat caataaatcg cctttacgta
    26821 ctttttttcc atcagatact gcactttgaa agtatttgcc atttaaattc actgtatcaa
    26881 taccgatatg gatcagtatc tctgctcctt cttcactgac taccccaatt gcatgtttcg
    26941 ttggaaaaac tgttgaaact tccccgttta cagaactgta gatttttccg tccgttggaa
    27001 tgatcgcaaa cccttctcct aaagatccac tagcaaatgt tggatcgttt acttcagata
    27061 aatcaattat ttcacctgct gcaggagtac ttaacactat atcctttgag gattcttctt
    27121 cgatttcatc cttgtagccg aaaagaaaag ttagcataca ggaactgatc atcgctaaga
    27181 aataactagc aactagccaa acaaatcctt caccaatata aatcgggaaa gacaaaatac
    27241 ttcttgtagc aactgccaaa gcaccggctc ctgcggcacc agctactcca ccaaatatag
    27301 ctccacatat acaagcaatg taaaatggtc gtttatattt aagagtcaca ccataaacag
    27361 caggttcagt gattccaaat aatgctgtga taaatgcaga taaagataaa ctttttactt
    27421 tcttattttt cgttttcaaa aataccccaa gtactgctcc tgcttgcgca gctacggcag
    27481 tcatacatgc tggacctagc gtatcacgac cgtagaaaga aagattgttg gtcattactg
    27541 gtacaagtcc ccaatgtaac ccaaaaatca ctaacacttg ccaaaatccg ccgataacag
    27601 caccagataa aacagggcta aagttataaa cagctaaata tccagctgca atcagctgac
    27661 ttgcataatc tgcaattgga cctacaacta ataaaataat tgggaccatc acgatgatac
    27721 aaatgaatgg tgtcaataaa tttcgaatac tttcgtgcaa gcttttgcga atcttaggct
    27781 caatataaga aagaacccat acagctaaaa ttgctggaat aaccgtactt gtataccttg
    27841 ctagaatcac gggcatccct agaaatcgca aggttatgct atcactatat gcactgacaa
    27901 tcgtaggata aacgagagct gctgcaataa ccatagcgat gaaacgatct gtattgaact
    27961 tttttgcagc agtatatgcc aaaaatatag gcaagaaata gaaaaatgca tcagctgctg
    28021 cattcaaaat gatataagtt cctgattcag tagttaataa attcaaactc gtacataagg
    28081 cgagtactcc tttgatcaaa ccacttcctg ccaagacgcc taaagtcggg gcaaaaacag
    28141 cggcgatcat gtctataaaa gagtttaaaa tgcctttttt ctcagttcct ctctttccac
    28201 tagatgcatc atcttctagt ttcatttgtt tggataatac gtcatacact tcatttactg
    28261 catttcctac tacgacttga aacattcctc cagcttcgac tacagtgatc acaccttcta
    28321 gttgctctac cttctcctta tctgccttct tattatcttt tagggtaaag cgtaaccttg
    28381 ttgcacaatg gtaaacattg ctgacatttt cttcgcctcc gacaaatcgg acaatatctt
    28441 tcgctaattg tgaatagtcc atttttactt cccccttgtt aattaatgcc gtagcaatcc
    28501 ttcttacaag ataagtatag ttcttagaaa acggtttctc cattcaatta gtaccccttc
    28561 taatagcgaa cgaaactgta caatagtgtg tatggtttac ttagccgagc aaatcactca
    28621 ctgcttgaaa gaaaacaagt atatcgtttc tcttatagtc aaaggagaca aaaaaaacac
    28681 gcttgatctt cctttgctga tgtataagtg gtatctcaag ctgatactct gtgatttttg
    28741 tttctacttg gcttttgata agacagcaca acaaagtctc ggcatttaac ggaagcataa
    28801 ctaggtcaga actaggagag attagttgct gaatggaatc tatcttttta tggattttta
    28861 tttgattttc ttctttgtag taatgatcaa gcagttttgt tttctttttt tcttcaacta
    28921 tggtcagctt ttcacgcaaa atctgataaa tctcccaaga ttcaaactca tctagaacac
    28981 taggtagata aatcaaatca tcccactcaa aaataagctc ttgctcagta gtcaaaattg
    29041 catcatattc cgttattttg tttgattgat ttttgaaaag atacactggt tctatccggc
    29101 attctttgat ccacatgttc atactttgtt gaatctgttt ttttagtgta ttcaaaatac
    29161 taattggctg gttactaaca atcagtatct ccccttgttt tcgaaaagga ttcaacgctt
    29221 cggcaagata caaaacaaaa taagcgcttt cagctaaatt gggacgtaat tgaaaatgta
    29281 ttgggaagaa acgccgaatt aaataaattt ctaaaggata acgggagatg atcttttgta
    29341 caaaatgatt catggtatta tgtccggcat ccatcctcgt tttcatttgt ttgatatggg
    29401 ttagcaaaaa ttctgtttca tggaacaata acgaaacagg aagatctaaa aaacgaataa
    29461 tcgtttgctc aaatgctctt agattcttct ctatttcttt gtttttttca tcattaatca
    29521 tcaaataatt taactctaat agtcgatttt caattgcttg tttttcttga ttggaaaaag
    29581 aatagggtag ttctgatgat aacttttttg tgagggtctc taccatcgga ataaataatg
    29641 tatacctgtt tgttttttct aaaagttctt ctcttcgaag aatggtcaaa acgagatacc
    29701 gagaaaatcg tatgaagtcc tctcctgaaa caatataatt atatgatata agaacctctt
    29761 ttaatgtctt ttggacagtc ggcatgatat tggaaaaaat tgtactgatt tgttgaggaa
    29821 accttgcgtg ttggatcacc gtttctgttc caacaagaga taagaaaatt ctttttgaat
    29881 tttcttcagc aatgattttt agtccttttg tcgacgaaac ttccagttct aatttaggat
    29941 tgcgctcgat ccaacgcaaa atcgttttta tttgtaatga tacggctgtt tttgaaagaa
    30001 agaaaatgtc tgctatcttt tgcatactga catacccttt ttgaaacaat aagtacgtca
    30061 caatagttga agctcggaaa tccatgctgg aatagatttt atgtttcgtc acctcttgta
    30121 tgatttgctg tttagtttca gattcaattg attctaaaaa ataacctgaa cgtttatttg
    30181 actggataat gattttcttc tcgtagatag caagcatatt tttgatttca gtttggatcg
    30241 ttttcttgtt ggtacctaaa gcttcagcta attcttcgct tgttatccaa tgattcttat
    30301 ttcttaatac tacaagaatt tggagttgtc gtttatttaa gtccatttgc ttacacctcc
    30361 tctttgttat aaacatacag gaaaaacctc tcaaatgaca atagtcactt gcagaggttt
    30421 aaaaatcatt tgaagaaaga tctatattca aggtttttac cattagactg gataatttgt
    30481 ttataatatt caaaactatc ttttttcaat ctcctaaagc ttccgctccc atcattttgt
    30541 ctatccacat aaataaagcc atatcgcttc ctcatttccc ctgttcctgc cgatacgata
    30601 tcgattggtc cccaccataa gtaaccaatg atttggcaac catcttttat tgcctcagca
    30661 atttcctgaa gatgtatttt tagatactca caacgttttg gatcgtggat tcgattcaat
    30721 tcatctggtg cttcatctag tccaatgcca ttttcaacta tgaataatgg taattcataa
    30781 cggtctgtca attcattcat gacataacga aaccctagag gatcaattgg ccaactccaa
    30841 ggttcgggag atttttcctt caaaaaggga ttatcgaaac catttgctcc gccagtatct
    30901 acgcgcattt ctgcttcttt tttatagaca gcgctacgat aataactaaa tgcaatataa
    30961 tctgccgtgt tagcttgcaa gcactgttcg tctccatcta acattactgg tgcacagtct
    31021 tcttccttcc atgtcctgta aatataagct ggataatgcc ctcggcacat gacatcggag
    31081 aaaaaccatg aattgcgttt aaattgcagt gaaccaaata cattctctgg atcacagtca
    31141 tatgcataag tcgctaatgc agaaaagcta cacataatac ccatcttagc atttggatcg
    31201 attttcttta ataattgagt agcacgggcg ttcgctagaa acatatgatg acaggcttga
    31261 taaatcactt tttttgttag tgtagcaatg ggttcaagtt tatctctagc agggaatgta
    31321 accaatccag cagctgcata aggcatacga aaagcattat tgatctcatt aaaggtcatc
    31381 caatatttca ctttcccttg gtagcgttca aagacggtct gcgcataaaa ttcaaaaaag
    31441 tcgattaatt ttcgattttg ccacccccca tactctccta ccaatgctaa gggggtttca
    31501 taatgactaa gagtaattac tggctcgata tcatatcgat ttaatgtatt gattagtctg
    31561 tcataaaata cgagaccagc ctcatttggc ttctgttcgt ctcctcttgg aaatattcgt
    31621 gcccatgaga tgcttgttcg aaaagctttt aaacctaact ccttcagtaa acgaatatcc
    31681 tcttcaaatc gatgataaaa atcaatcgct tcatgagaaa gataatggag tgattcgtct
    31741 aaagcaatct cccaaatctt tttttcttca ttccactgaa tactaggatg ctggttcatg
    31801 atcccgataa gcgtatctgt cacattaggt aatttgccgt cttccaacca tgcaccttca
    31861 cattgattag cagctactgc tccgccccat aaaaaatttt ctggaaatat ttcctgatcc
    31921 ataactatct ctcctcgcta tcaacaattt cttacttgaa gtatagctcg attcatcaaa
    31981 acaagatatt ctaactattt cggcagaaga gaagaaaaaa attgaatgct actcttcatt
    32041 tttaatattt atatataaca aatacataag acgatccccg aaagaaaaca ctcgttaacc
    32101 atctaagcaa acgatttatt ttacttccaa cacaaatgct tcataaggtc gtaaatcaac
    32161 tttgccgtct ggtaactgtt tcagaggata atttgacaca ataaccctta aagcttcacg
    32221 tggaatagtg aacgtctgct tttcactgga catattacaa ataacgagcc attgctggtc
    32281 ttcatatatg cgagaatagg caaagacatg ctcaggcgtc ttttccacta attcatagtc
    32341 tccccagacg atgatgtcat gttttctacg caaatcgatc aatttcttgt acatataaaa
    32401 aacagaatct tgatctgcta acgcttcttc tgcattgatt tctttataat ttggattgac
    32461 tcgaagccat ggaattccag tagtaaaccc agcatgttca cgggcgttcc attgcatagg
    32521 cgtcctagca ttgtctcttc ctttcgcctt gattgattcc aagatttcct cttttgtaaa
    32581 tccagaagaa atcctttcgt tatacatatt gattgtctca atatcttttg cttccgcgat
    32641 atcatcgatt gggcaattcg tcatgcctaa ctcttctcct tgatagatat aaggggtgcc
    32701 tttcatcata tggagcaaga tagcaaacat cttggcactt tcttttcgat acacatcgtc
    32761 atttccccaa cgagaaacaa ttcgcggaag atcgtgatta ttccaaaata aactattcca
    32821 tccttcatca cctaaagatg tttgccattt ggataatact tttttcagtt cagagatact
    32881 caagggtttc aaatcccatt tgtctttgcc ttcttgctga tctaatccta tgtgttcgaa
    32941 ttggaagacc atggataatt catttctttt aggattggaa taaagtttag cgatttcggt
    33001 agttgctccc catgtttcgc caacagtcat cagatttttg tcaccaaaag ttgcttgatt
    33061 catttcctga agatactcat gcagtttagg tccattactt gtgatttctt tatccggttc
    33121 tttgccaatc atatcgatca cgtccatacg aaagccccca atacctttat cgatccagaa
    33181 attcatcatt tgataaatct cttggcgaac ctgtttattt ttccaattca aatcaggttg
    33241 acgcttgcta tacaagtgca gaaaatattg ccccgttttt tcatccaact gccaagcaga
    33301 accactgaat attgagcgca atctattagg ctcttttcca ttgaccggat cacgccaaat
    33361 ataatagtcc cgataaggat tttctttatt tttacgggcc tcaatgaacc acggatgttc
    33421 atcagaagta tgattcacca ccagatccat gatgatacgg atatttcttt tctttgcttc
    33481 ttcaatcagt tcttccattt cttccatcgt accgaattct gccatgattg cttgataatt
    33541 actgatatca tacccattgt catcattcgg agactcgtat actggagaaa gccaaattgc
    33601 atcaatacct aaatacgcca gataatccaa cctggaaata atcccccgca aatctccaat
    33661 accatcttga tttgaatctt gaaaacttct tggatagatt tgataaacaa ctgaatggtg
    33721 ccaccatttt tgttccatga ttttcctcct ataaatggat gtttctattt tttgcttaat
    33781 catttatttt agtctctctc actaaaaatt gtaacgcttt caaatagcta ttgcaccttg
    33841 ttatcggtaa cagaataaaa caaaaaaaca gctgaggaaa ttccctcaac tgttttttga
    33901 aaggattgat ttatgcaggg aagaatgctg atttcacaac ttctttataa acggtgttca
    33961 ctcagctgtc ccttggtatt agctcaaaaa acggcaaaat ataagaaact attttttcgt
    34021 ttttttctct aatactcagg ccagaacgct gatttcacaa cctctttatt tcacattcaa
    34081 tatccacatg aatcccacaa atacaaagaa cagaccatac atgattggag atacttcttt
    34141 tcctctttta gctgctaaca tggtaattgg atagaagata aaacctaatg caatcccatc
    34201 tgaaatacta taagtcaaag gcatgccgat caagatcaag aatgaaggga tcgcaatctc
    34261 taagtttccc cattcgattt gtcgcaaaga ttgtgccatc aatactccta ccataatcaa
    34321 tgctggtgca gttacttgtg aagtaacaac tgatagtaat ggagaaaaga acaagcctaa
    34381 caagaagaaa atccctgtag taatagctgt gatacctgaa cgtcctccaa cggcgatacc
    34441 agctgatgat tctacaaatg caccaacagg agaagtacca aataaggaac ccgcaagcat
    34501 agctgttgaa tcagaagcta aagctttccc tacacgaggc atcttgttgt ctttcatgaa
    34561 acctgcttgg tttgctaatc caactaaagt tcctgctgta tcaaagaaag tcacaagcaa
    34621 gaatgtcagc actacgaccc acatttgaag agaattgatg tcccctacat gttttaatgc
    34681 aactaaaaat gttggtttca aacttggtgc tgcggcaaca atttcagaag gcattggaat
    34741 caaacctgtg accagaccta aaatcgttgt agctgccatc ccgatgaaga tgccacctgg
    34801 tacacgccga acaagcaaga tagcagttac gactaatcca aaaatcgtca accaagtaga
    34861 accaacgttg agtgatccta atcctactaa tgtagattca ttcgaaacga tgatcccacc
    34921 ttcgctcaga ccaaggaaag cgataaatag accgataccg ccagaaatcg catatttcaa
    34981 atctgctggg atcgcatcaa tgatcatttc ccgcagcttg aagattgtga tcaaaataaa
    35041 aatcaaagaa gcaacgaata cgccggctaa tgctgtttcc catggaatcc ccattcctaa
    35101 acaaacagaa taagcaaaaa aagcattgat cccaagagca ggtgccgttc caattggata
    35161 acgggctaat acccccatta aaacacagcc gatcgcactg gctaatgcag tcgcagtaaa
    35221 cactgctcct tcatccattc cagaagcacc taatacagtt ggatttacaa ataagatata
    35281 agccattgag ataaatgttg taaatcctgc cagtacctca cgttttaccg tggtgttcaa
    35341 tttttctatt tcgaagtagg aaagtatttt ttccttcatc ttccatcccc ctaaatccga
    35401 acattataaa taaagtccgt taaaatttcg atttattaat aaaacaacgt gttttatcat
    35461 atactaaaac aaaacataag taaacaaaaa attcggattt taagcaaaat ccgaacgttc
    35521 catctgatta aatcccttta tctcaaagtt catgaacatt cactttggat tacaagaatt
    35581 tctttattgt gttaataaag tttcttcatt ctagtctcaa aaatcaatag tgtgatttga
    35641 ttttaccatt tcgtttctgt tcttctttga tgaattcttt tgtatcgaca ggattcagat
    35701 tatttagtcc gccaaaacct gtatcgacca tttttcccca gccttttgtc ttcttttttc
    35761 ctgatttgag attctttgct cgaagcttct cactatgttt tgctcgccga tacttcaaat
    35821 aaccttctaa tcttcccatt agatccactg tttttcctcc tttttattca atcagacgac
    35881 tcagcttttc ctataacaaa ataattgcct tcagggtcag aaaaattgaa agtcaagatg
    35941 ccattgttat ctgtgatttc tcctggttct tccatgttct cgtagatagt ttcaaattca
    36001 tccgtaaaaa atactaaaga aggcggtggt cctaatacct ctggtgaaaa cttctgcaca
    36061 aatacttttg ggaatacgcc taattctaat tcttttgaga tgctgagtac aatactctta
    36121 aattcttctg gcaattcgat tgtttcagaa atttctgcac caattttttc tacaaagaat
    36181 cggcaaatca tctctacgtc attgacataa agcatcacac gtgttttttg tatcatcatt
    36241 tgacccttct ttctatttga ttttctttcc tgtctataaa ctgattccat tatatcacga
    36301 aagcgcaaac accttgtgtt tgatactatt caagaaaaat aaagcaatag tttctaggat
    36361 tgctttcctc cacaaacttt ttttacaaaa aaaaaacggc aatgaatgcc gctcttctta
    36421 tttcgattct tttataattc tgcttctgat tcatctttgg ttagtgtgtg tctaacacta
    36481 gttacgccaa atgtttgatc gattttttcc ttcaacgctt cgattgcctg tgcgccacgt
    36541 gccgcttgtt cagcattgat ggattcaatg accagcaccg ctgcttgtgt ttcttccgtc
    36601 agcatcgttc tttgggcttc acgccagttg agacagcgac agatcgctcc ttgttcatca
    36661 tagtagatga tttcttccgg aagtgccgga gcatcggaat ctgcacctaa aggcaggaat
    36721 gattcgccac cttgggcttt tcctaaatgc agatttcctg cgatcttttc gatatcttct
    36781 ccgccgcaag gtacgccaaa agacaaggaa atactattat agatatctac aagcggattg
    36841 attgggaaaa attcccgttc ttgatttgca cgtttcagta aagcttcaat agaagaacgt
    36901 gcaccttttt tagtcttgaa tgtggtaaat gcctgacgcc actgagcaat gacttcgttt
    36961 tggctgaatg tttcttctgt aaggtaattt cttgcttctt ttgctgcctc attcaataat
    37021 tgatgatacg tttcttcact ttcttttgtt acgtggttat cgataccatt tacggataag
    37081 atttcgatgg ttgcttcagg aaatacttcc cagaaattgg tatccactat tacttttttc
    37141 atctgttcac acctcttgat tagaataatt gcttgatgaa tagatagtct gtttggggat
    37201 catctcccat ataaaaaaca tgttcgctgt atttgataaa tcctttttta cgataaaaag
    37261 cgagcgcaac atcgttgtgt tcccaaacac cgagccaaat ggctgttttt tgcttttctt
    37321 ttgctttttc aatagctgta tctagtaaaa atgtaccgat accttgatgt ttaaattttt
    37381 ttcgtacata gattcgctcg atttctaaaa aatgatcagc catttcctca gattgactcg
    37441 ttcctgtatt tagtttcagg taacccgcag gctcttgatc gatactcact aggaaaaaat
    37501 cggtttcttt atcttttagc tcttctgtaa gtttttctag tgcataagct tgtgtcaagt
    37561 aatcagctaa atgctccggt gaattatctt ttccaaatgt atctgtaaat gtttcgatac
    37621 taatttgttg tagtaagtct acatctgaag gcgtcatttt tcgaatttcg tatttattca
    37681 cggcttcttc ctttcaatag atccttttat tgcctttttt gacaaaattc caatcttcgc
    37741 taacattctt gcatacttta tttaaaagat attccaattg ttttgcttct tcgtcagaaa
    37801 gaccatttaa tgcgactgca ttggagtagt tgttttctcg aatgatgaaa ggatagattt
    37861 ctgctccttt tttagtcgga tagagtttct tgattttttt attttcttta tcttccaaac
    37921 gttcgatcat cccattggat tctaatttct tgattgctcg ggcagtcgtt gtacgatcta
    37981 ctttgatcat ttctgctagt ttttcttgga tgattccagg attttcgcaa atacgtacta
    38041 aataaagata ctgtcctctt gttaaatcga cttctttgaa ttcgatattg ctaatagaat
    38101 ctagcgctcg ggcaatcatt ccgatgtctc gaaggatttc agccatttca tcccaccttt
    38161 attttgtttg taagactagt tcacctcttt ttgttgcaaa tgcaactatt ttcaataaaa
    38221 aaatcaagtt cttttttaat aactagtaaa gtatattctt ttcttctttt acgaaaaagc
    38281 tcaatcacaa tagatctggt ttttgttttc taaaataagt aactgtttct tcgttgttcg
    38341 cttttctgat agctttggca actcttctat actaaaaaat cccatgttgg aagtttcatt
    38401 attctcaata aacttggcct cttcaccgtg gatcgcacaa gcaaaaacaa gtttataata
    38461 ctgaaaaagc tgtggaatat ctttttgttt attcgtatca aaaactgctc gcaactgagt
    38521 agtttctact gttaatcctg tttcttcata gacttcttta cgcacatttt cttcaggaga
    38581 caatcctatt tcagcaaatc caccaggaag tgaccattct ttcgtccgtt tatcttcgat
    38641 tagtaggacc ttgccatctt ttttgatata ggcacgaaca tctacttttg gtgttggatg
    38701 tccttctcca atcgttgtga gcgcttttat ttcttcgaaa gtttcttttc ctatttcact
    38761 gatcagttcc aatgaaatcg tccggatctc ctcaaaacgc tctttatcaa atacatcttt
    38821 tccataatat agaccagctt gagaaagtgc gagtagtctt ttgtattgag ccaacttctt
    38881 gttcattctc ttcccttctt ttttagcaaa tgtatgacta tgtaatcaag tatagaagac
    38941 tggtttaatt acggcaaata aatataatga aatctgcaat aaataaaaga atgttaaaaa
    39001 actcgagaac aaaagacatt cgacttctct ggggctgtga ataaatggcg ttcaaaagct
    39061 tgcatctgcg gtaataagcc ccaccaaacg aaaaatatga cgagctattc ttgtctggct
    39121 gttcttatta attgaggcag gacagctttt tcacaacctc cccactaaac ggtgttctcc
    39181 taccaactcc tcggtcacaa gtcacaatat tcatcaaaca tgagaagctg tttttttgaa
    39241 attgctcctt gtgactcgga gctgaacgac aggctcacga cctctcggta aacagcgttc
    39301 aaaagccaat ttttcgatat taagccattt ctttgttact tattgtcatt gatttctttt
    39361 tctaatttat ccaatagtgg agtgtaatcc acttgagtat agtctgttac atcaaattca
    39421 tatagtgttc ctaactggaa tttgtcatat tttcggtcgg cgatgatctt tttgaatgct
    39481 tctttcgtaa tatgattggt ccctttcgtt cgatcttcca atacgtcacc atagtgataa
    39541 tgatccatga tatagctcaa atgacgatct gattcacgat cacgtttgta ccgacgttgc
    39601 cataacactt caaagtctgc tgttaggcga atcgtgatca cctgatatcc gtatctatct
    39661 gctaattctt ggagtcgtgc tttttgctta tcactaaaag gatactcgga cacaatcact
    39721 ttctttcctg cttccatata caaatcaagt gctttgtaat agaaaggata aactttcgtc
    39781 ctttcttgtt ccgcccgttc ttccaaattg ttaaacccga tggattctgc atagtattcc
    39841 ttgatttcat cgagcgtgat ggcatacata tctggaaatc gttcccgtag tttattcata
    39901 agatatgttt ttcctgtacc aggactacca gccagaagaa tgaaatactt tttcatctca
    39961 tttatttcct aagttaattg gactttccaa gttcataaac agtccaatct tatgcttgac
    40021 aatttctttt actgcttcat ttgctggaat cacattatgg cgaagagact tgttgatttc
    40081 tgtttcacta aaggtttctt tagctacttt cgtccagttg actaaaagct ccgttccaac
    40141 attgaatttg cggatcccgt tattgatcaa ttcaggataa tcttcttctt tcacacctgt
    40201 tcctccatgg ataactaggg ggacatcaac tactgcatcg atttctttca gcaagggaat
    40261 gtttacttct gtttttgatt tgaattgtcc atgattcgta ccgatagcaa tagctaatgc
    40321 atccacgcct gtagcttcta caaatttcac tgcgtcttcc ggtcttgtat ataccttgtc
    40381 attttcagcg acatggattc cttcttctgt accgccgatt gttccaagtt ctccttctac
    40441 ggaaacaccg tgcgcatggg catattctac tactgctttt gtcttcagga tattttcttt
    40501 gaaaggcaga tgggacccat catacatgac agaagtatat ccgctgtcga ttgcttcttt
    40561 gatatcatca aagtctcggg cgtggtctaa gtgcaagact gcatcaacca tttcttcttc
    40621 tgcaatagct gtcattacat tgactaacac atgcatgccg atatatttag cggtatcgac
    40681 acttgtttga ataatgatcg gcgaacccat ttcttttgct gcgcctagca tagcaggaag
    40741 catttctagg ttatgcgtat taaaagctcc aactgtaaaa ttcaactctt ctgctgtttt
    40801 tgttacttct ttcaatgttg tatacatctt ttattgccac cttatctttt tatttgtttc
    40861 ctttgatcgt tacttgtttt gcaatttgtc gcatctcatc cattttgccc atgtagtaat
    40921 cgatcccttg atcatcacca gcacgagcag catctgcacg cttctggtta tagaggctca
    40981 tcagtttttt atacccatct ccgatcgtct gtttattttc caaactcttt tctaaatatt
    41041 ggatggcttg atcgttcaca tacaattcag aaaatttaag tccaacttgt tcataaagtt
    41101 tcgctaattt atgcttatct tgggtagtgg aaatctcttg tttcaattgt gtaatctctg
    41161 attgaatctt ttcggtcgct tctggcgata gggctttttc ttcttttgcc agaacttttt
    41221 tcggttcttt ttcttttttc ttaaaaaatc caaacatcat tttccctact ttcaattaaa
    41281 atgctttcaa aactgggctt aagatccagg catataagag tgctgcggct actgtatcta
    41341 catcggttgc tgtagcattg ttgaatccca tcgaattaaa gatcgttact agcaaagcgg
    41401 gcaataatgt aataaagaaa ccatgagcaa taccgccaat gatcgctcct cggcgtccgc
    41461 caactgcgtt gccaaatatc cctgctgtcc ctccggcaaa gaaattggtt agcataccag
    41521 gtaaaatcat cgctaagccg aacatgggca aagtaaacat ggcaataatc gtaccaatcg
    41581 ttgtcgtaat gaaacctaaa atgacagcat ttgggctata aggaaagaaa actggacaat
    41641 ctaaggcagg gatcgcatca ggaactaatt tcatagctat ccctcgaaat gccggcacga
    41701 tttcacctaa cagtagacgt acaccagcta gcaatacata cacacccact acgaattgga
    41761 tggcttgtaa aaaggcaaac atgatatagt tttgatctcc tgataattca gaagcgaatg
    41821 gttcgcctgc aaataaaaca gtgatgatat atagcgggac catcacgacc atgacagaca
    41881 aataagtatc ttgcagaaat tcgaaatgtg tcggcaactt gatatcttcg atcgactttt
    41941 tattttcgcc tttctcacca aaaagcttag caacaccggc ttcaaataag tacccgatcg
    42001 tacaaaaatg acctaaagca atatcatttg atccagtgat tttacgaatg atcggttgcg
    42061 ctactgccgg catagcaatg gcgaaacaag ctccgataaa accaccgacc aagatcaata
    42121 cgatccctcg cagaccggca aaatagccaa agactgtcgt catcgttgcc atccataaaa
    42181 ttgcttgacc agttaaaaaa atgtacttcc attttgtgaa acgagccaaa ataatattga
    42241 atacaaaaat ggctaagaac gtcagtgcaa tatctctacc taaacccaaa tcattcattg
    42301 cttgaccatt gatagattca atagaaggga tgatcccttg catatgaaat ccttcagtaa
    42361 agattttccc gaaataagtt aaacttccaa cgatgatgct cgaaccggca ctcaacactt
    42421 gaaatcctag gagtgtcttt aacgtgccag agatgacttg tcctgctgat ttcttctgca
    42481 gtagtagacc caacatagca atcaatgcaa tggtgatcga tgcctgagtc aaaatatttt
    42541 cgatgataaa attgataacg cccatgtttt taccctcttt tctttttgta tttagataac
    42601 tcccttatct tgcagaactg gggtgatttt ttcttcaatt tcattttttg aaacaatatt
    42661 tttcaaatag atcatcgccg ttttaggatc aactgcgaac ttttcgaact gcgaacgaaa
    42721 attttctgct gtgatgatca catctggttt catggaaact gcggaagaaa tatcacaatg
    42781 gtccagtttg gcttctactc ctaatttgtt caaaacatcc tcagcagaca tctgtgctgc
    42841 aaaactactt cctaaacctg caccgcatac gaataaaatg ttcaatttgt tcatctttgt
    42901 tctcccccgt atcatttatt atttctcaac tactgaaaaa agcagttgtt tgaaatcttc
    42961 gatatttgtg cattcaatca atcgatccaa cttctcttcg ttgttgatca attcaattag
    43021 ttctttcatg atgttcagat gcgagtagga gtctactgct gccaaacaaa aaataatctt
    43081 tactggatcc atttcaggat taccgaaatc gattggttgc tcaatcgtag ctacgctgac
    43141 tcctaattta ttgaccccat cttctggtcg ggcatgggct agtgccatgt gtttgccaat
    43201 cacgatatac ggtccgtaat cattcactgc acgaatcatc gcttcgacat agcgctcttc
    43261 gacaatgttt tctttaacca atggtctagc agctagttca atcgattcct cccaagtagt
    43321 ggctttttct ttgatcatga tattgctgtc cgtcaaaata tcttttaaca taggctggat
    43381 ctcccttttg ttgatttcta aattgtttgt ttcgaataat ttttctaact tgccatagat
    43441 gattcgatct actcggccac cactttcttt catgacatct attatttgag tgaataactc
    43501 cgtcgattca tgactgttag gctctaaccg ctgtagttct tcatttcttt tcaaaaagtc
    43561 ttctataacc aagatattcg tctcagttaa aatcggatcg acaactaaaa gcggcttatt
    43621 tcgataattc aattgaaagg tgctgaaaac cagattgaca tccaatttat ctactaattc
    43681 gatttctttt gaacttaaaa ctgcagtaat ctcgatctga ggaaactttt ctttcagatt
    43741 ctctgctaac agatttccag tagccatccc atggttacag acaacgactg atttataaac
    43801 ataattcaag tctcgcttga ttacactggc gatcgtcgaa aagtggatga caagaaaagc
    43861 tatctcgtcc tccagtatct tgccaccaat tacttcttcg atcgtaggaa caaaatgagt
    43921 gactgcgcgg tagattggga aataatttgt tcggatgttt tctttcaatg gattcatgac
    43981 ttggatatga ttcttgattc ttacaatcag tgcagccatg tgcttataga gattttcgaa
    44041 taaagcttct tcctttagat ggaaaggtac gtgtgtctcg ttctctacaa attggatcaa
    44101 ttggatcgtt agtaattgag cttgtaccca ctcgattgaa ttggagatat ctctagcatt
    44161 gaacgtctca atagtgaaac gaagataatt tctttctact tgcgggacat tgtccaattg
    44221 aaattcctcg atcatgtggt caataaatgc agaaaagcta ctgtctttta tttctgtatt
    44281 tttccaatta atggcagaga taaattcatg tttcctgatt cgctggatcc agatcaatgt
    44341 aaaaagcagg atttgttttt tataaatatc atcttcttgt ttcaacaact tattggaata
    44401 gatctcctgt atttttttga tttccaccaa aggaaaataa tggagaaaga ttttttgagc
    44461 cggtgttaat tgtgcatctt gattcataaa gaagtcggtc attcccagat tctgattgat
    44521 aaaatcatag atcatcgtac ggatagatcg ttctgaacct ttataaacca gtccttgctt
    44581 tccataactg acgatctcta tcccataact gaccagtgtt gctcgtaacc gtctcatatc
    44641 ttcatctaat gtacttttag agatttgaaa ttcttcttct tttttgttta gatggatcgg
    44701 gattcggctg taagcaacgg ctaaaactag atcaaaaata cgttcgcgcc gattcaaaaa
    44761 gccgtctttc acatctttta tttcagtttc taatcgagct tgcttttcct tttccagctt
    44821 caacaaaaaa cctttgttgc gaattgtacg gatctccgga agctttttac tgctcaacca
    44881 gatattcgct tcaagaactt cctgacggat cgtccgctga ctgacattga actcacttgc
    44941 tagatgtttt gcttttactg gatattgggc tgtcatgatt ctgtgaagta tttcaattgt
    45001 tcttgttttc atagaaatga tctccttctg atttcaagta tatggttccg cttacataat
    45061 aaaaagtaga tatttctgcc acttgaagtg gaaagaaagt aagcaaagca aacaaaaaat
    45121 aagccatatg ctttttcatc tcctcaacga aaaagacata cgcctattat tacaaaaatt
    45181 ttgcccatct atacaaaaaa gaggttgtga aaaagctgtc ctgcatcaag taataagaac
    45241 aactaaacta aaatagtgct tcctattttt cgtttggttg ttcttattat cgcagatgca
    45301 agcttttaaa caccatttag tggagaggtt gtgacagaga tgttcagcct tgagcgataa
    45361 gaagcgattt tgaaaaatcg cttcccatat tttacaaaat cgtgccttat tgccgatagg
    45421 ctatctctgg cacaccgttt attcagaaaa aggagctgcg acaaggcttt tgtcacaact
    45481 ccgttcataa atatttcctg tcaatccagc ataatcggct gaataaaatc ggcttgccat
    45541 cttttttctt tctcagaaaa atcaaaatac catatctgag aaaaatactg tcgttgtgtc
    45601 ttacttcctg agatcaatcg cccatctttt cgatcgactg aataatcaca gcatctgaaa
    45661 tcaattcgga tggcaaaact attttcgtgg atctgtcgat aatttcctac ttcctgaata
    45721 gttatttttt tagtatgatt tcttacaccg tctgctttgt ttttttgcag aactttctga
    45781 tgttcggcaa acagcttgtc tgtataacag cttctggctt tttcaggagt ttcttcttcc
    45841 caagcggatt ggatcttcat gaatatcttt tggatttcca gaaactgtgc tttcttttct
    45901 ttagccgttc ctgacatgcg ggacaatatc tccgctttca gccttttctt ttgtctttta
    45961 ttcttgacga tagaaaaagc agaaacccct cctactgaca aaacagctaa tgcaagaaat
    46021 cccgtttgct cataactaga aggacctgta cctggaccat aatacccata agaatctctg
    46081 ccgtaagaac gactgccaaa ataactgccc ccatatgaac gaccaccaaa tgaacctctg
    46141 cccgaacttc gaccgctaga tgtacctcca ccatgaccac ccgcgacagc gaatccttgg
    46201 gtcggcagag caaacaaaca taggcccact aacaaaatga ttgctaaata attgattttt
    46261 ttcattgtct tcctcgttat cttttattac aaaaaactat ttttatgata gccttgctaa
    46321 aaggaagagt caataaccag atgcataaac tgttttttta gcaattttgt cttatttagt
    46381 cattgagtaa catcggacaa atgtatgtgt cggcagtcca taattcctct atcttcagca
    46441 tgaacaagct tgcagacaag agatctgcgc ttcctccagg agagatttgg ttttcctgac
    46501 aaaagtgcaa cagtgctgta aatggacgat ttggtaaagc actttgacta gcagcatgag
    46561 caagtttttg catttccact aacgttttct gatcactgcg atgcaatacg catgtgtcat
    46621 ctagcgaact catcaaaact aacaacatat gcatttgttt ctgtacatat gaatcattat
    46681 ctagctcctt agctgcttga attgcaagcc tgatatgcgg ataccctaat atcgcttctt
    46741 catatgcacc atttacgcca tatttttgct tcaccctatg accatgagtg gtttttttat
    46801 gacgatattt tgtatcagga aaagcagcaa gcttgctgac atcatcaagt attttcgata
    46861 aagaaaaagg ctgttccata ctgatttttt gtgcaatgac actgatcaat agccccattg
    46921 cccaaatcgc tcctttatgg gtattgatgc catttgtagc aatcagcatt gccttttccg
    46981 cttttcgccc aatctcagca attctttctc ttaactcctg atcaatagca tgatgccatg
    47041 ctatttgagc catttcttca aaaaaaggag ttagcgtctt agcagactta agaaataaag
    47101 catagtccat atcggaatgt gcacccgcat ccagtgcatc cactaatcca ggttttggac
    47161 ttaatgttac ttctgcgatc aatgcttcct ctaccaacgc agcgagttgc tgagtcactg
    47221 ctttttttcg ttcgtcattt atcatgacac tcactccttc ttcctactat tacttgctga
    47281 aacctttttt gatagctacc caaatgatat gaagtaacca gcttaaagca aatgcacaac
    47341 ttgccgtcat tagataagct acaagaatca tgactcctga aggtaactgc agccaactat
    47401 ctttaaacaa taataacagt acttggaaaa caaatacatt ggctaaatac gtacgataag
    47461 cataaacaga caaccatttg aacaatggca attttggcga atcgtttaca atcattttgg
    47521 aagcgcctat gaagacagca aagatgatga ctaaactgta taaactttgg atcgtattca
    47581 agtaaggtgc attcgcaaag ctcaagtctc ctggatgtgc gagcaatgaa cggacagaaa
    47641 agaacgcgat aactaaggca acaggtaaga aaagataacg tactctgtcc aaatattgat
    47701 agactttttc gtgataaaca aatgcggcta ccccataaat catgaaacta gcaacaaacc
    47761 gatctaataa gtaccagctg tcatgataag gcccagtcaa tacttgtgtt tgatagaaca
    47821 gataccatgc gacatataaa atcgttgaac caatcaatac tggccataca tattttttat
    47881 tagcaaatac tttgtaaccg agccaaacaa aaaacggcat gaacaattgg atctgcagca
    47941 tcataacggt ataccataaa tgaggcgctc catcacctgt gacatatttc agtaaaaagc
    48001 ttgcagtatc cgtatacgga gtttgttgtt ggactgctgg aaatagccaa agataagccg
    48061 tcgtccatag aagatacgga acgactagtt caaaaaactt ttctttcaaa aattcccaat
    48121 aagacaaatg ctgacttgtt tttaccatat tataaacaat tgcaaaaata aacattggtg
    48181 ctgtgtattt aacgatgacg taaaaggtac caagaaaagc tgtatttgtt gcgctgtggc
    48241 tgtttgccaa agcaaagctc aatatcgttt gcatcataac agccgttaca gctgatactt
    48301 tctggaagtc acttacgttg attttgattt ttttgttttc aaacatctct ttctctctct
    48361 ttttctttat taccaaagtt ctttggttaa tttaggtcct tcgactgttt tcaatagaat
    48421 ttttttctct ggctctctca aatactcttt tagtgaaaaa ccgccttgcc gagtcactac
    48481 ttgggtatcg aattgctcaa aaaaagcagg atgcgattga attacctgaa ttggcaattc
    48541 aatcggtgaa tttgcgtaca gcagcaaatc aaaatcactg gtttctttta ctgccggaat
    48601 cccagtcgat aattcaaatc ctaagctgcc gccgattccc catttacatc ctgataatat
    48661 gtttctagca gcatgaaatt gctgataaac aggataatcc atgattttct cttgtctata
    48721 cgattcttgt tcgatgatgt cccacggatg aatgacttgt ttgacggcat gttttgagat
    48781 ttctgttgac catctctgat tgcgggcagg tccacgtaaa ccaatcggta gtatttctgc
    48841 gtgatccgtc gacgcacgac gaactgttcc gaacatcttt tcagttagaa cgacccattc
    48901 aggcaaggtg aacccttcta tttcatgttt atcaaaaagg acaatatcat gtgcttgaat
    48961 cattttttat gcatcccatt cttcgtccat tttggcacgg actttatttg tttcgacacg
    49021 tccgaatgtt ttggctgttt cgtttgtgta tcgatttgtc agcatcgtgt catttgtatt
    49081 gcggacatct tgaatggctt cattgattgc ttcgatgact ttgctgacat tttgttctga
    49141 agcagcttgg tcggtgatat cttctaggaa atgatagatc gctcctagtt ttgtataatt
    49201 attgatatcg taagcgatag ctggaacttt ttcagcagct gcttcgattt cagcaaccga
    49261 acgttttgtc acacgtgcag cactttcttt agacatcgct tggacggtaa tttctttatt
    49321 gtttaaagca atcaagcggc ttgattgtaa tccatgtgct aaaaatgcac ctgaaatagc
    49381 atttcctgca atcaatccaa taactggatg tccagcttga cggagttttg cataagcagc
    49441 agcactatta gccaaagcca catggatacc gatcaattct tcgttatagc cgtatgcttg
    49501 gcttggtaca tcgatcacaa cgatgattgg ccgtttcact tttttgtctt tatcttcttc
    49561 gtaaacgtga tccaaaattt gtcccatgcg gaaaccttca actaaaccaa cttcaccgtt
    49621 tcgtacacgg tacatcgggt ttttaggatc aggtacgatt gatgtcacga tggcttcttc
    49681 tatgaattta cttgatttac cataatacac agtactaata gaagaagttg gatttttcat
    49741 gccagttagt gccgcaaacc aatcgtaacc aacacttgct gcagctggtt catttccttc
    49801 tgctacttct ggtaagtcaa ttgattttgc ttgatggtta gcaattgctt cattgtaatc
    49861 gtcaatggcc attggttttg aaagatctaa agaatcaagt aatgataaat aaaatgctga
    49921 gctttcactg cgatgcgtgt cttttcgttc gtccattgct gcgatgacag cagctttgat
    49981 tgcttctaca ctatcagtca ctaattcatc aatgattttt gattctaccc gttgacgaga
    50041 tcctaatgta ttccaaatca agtctttgtc actagaatca aattctcgaa cgcctgcttc
    50101 ttgttcaata acttctggtc cattcaaacc gactctcgct ttttttgttg cgattaaata
    50161 actcattaat gctgaagtaa tcgacatacc gccaaatgat cctacacgtc ctggaatcaa
    50221 accgatgact ggtacatatt gttttagagc aatgattaca tttccgattt ctgaaataga
    50281 taacagtccg tagtttgctt cttgcaaacg aacaccacct gtatctagta caatgactgg
    50341 aaagatctca tttccttttt tattttcttc taacacatgt tcaagtgcag caacgatttt
    50401 tgcaccggaa acttcaccga taccgccacc ttggaatttt ccttccatag cgattactac
    50461 tgcttgtttg ccattgatcg tgccacggct gacaacgatc ccgtcatcac tttcagggac
    50521 gattccttgt gctggtaaac ttggcgcgat gatttgatcg aatggatcaa tcaattcacg
    50581 tccattgtca agtagagcgt tcactcgttc tctggcgttt aattctacaa aactattttt
    50641 catctgaaag ttcctccatt gcttgcgtca atcgtaggta cacaactcct ggtgtagcac
    50701 cgaagtcatt gatcacgata tctgctgaaa ttggataacg atcaaaaaat ctcgttaata
    50761 cattgttcca tacttcgccg aagccatcgc ttcctgtaca aatttttaca gtagattctg
    50821 ttttttctgt tggaaacatc aagatttcca aatctcctga accgaccact cctacatgga
    50881 ttttcttatt gatcggctgt ttcgttggat atttataagc taattgttcc atggatctct
    50941 cccctttttg taaaacagag catacacgtt ctgcttattc gtctttcttc ttccatctgg
    51001 gtaaatggcg acaaaaggtt tttctcgcca attttatgac caagttctga atttcattgg
    51061 tggttcatac aggccatctg accattcgat caaatcatcg atggtttgtg cagctaataa
    51121 tgaacgtttc gcttcactac gtttgatgcc tagatcttct ggtaatttca cgatgccggc
    51181 tgcacgcaag ctatctagtg attctttcgt gctagtcatt ccaagaggtg taacaccgcc
    51241 aattgctgca attgctgctt gtcgttcttc ttttgttttt gctttgtaca aataagcaat
    51301 tccttcttct gtcacgatat gtgtagtatc ttcgctataa atcatcactg gtgcattggc
    51361 taagcctgct tgtttttgaa cagctagtgc atctaattct tcgacaaaga ctggtttttt
    51421 atttgaacca tatgtttcaa ccatttgaac aactaatttt tggcctttcc ctaatggatc
    51481 atcattgtcg cgaagtgatt gccatgctgg tgtagaatga cgacgaccgc caggattatg
    51541 tcccatgtta ggcgcaccgc caaaaccaga taaacgtcct tttgttacgg tagaagagtt
    51601 tccttcgtaa tctaattgca aggttgaacc gataaacata tcgatggcat attgtcctgc
    51661 agcttgtgat aatgttcggt tactgcgcat cgtgccgtct ttaccgacaa aaaagacatc
    51721 tgggcgagca gcaatatatt tttccatacc aacttcgcca ccaaaactat ggatgctttc
    51781 aacccaacct gattcgatgg ctggaatcat cgttggatgt gggttcaata cccagttctt
    51841 cgcgattttc ccttttaacc ctagtgattc accataagtt ggcagtaaca attcgatagc
    51901 tgctgtattg aatccaatac cgtgattcaa agattgtacg ccgtgttctg cgtagattcc
    51961 tttgattgcc atcattgcca ttagaatttg caattctgtg atgttttgag gatctcgtgt
    52021 gaataaagct tctaattgat aaggtttatc tgctttcacg acaacgtcta cccagtcacc
    52081 agggatatct acacgtggta aagaatcgac aacttcgtta acttgaacga tcacgatacc
    52141 atctttgaaa gctgcagctt cgatgattgt tggtgtttct tccgtattga aacctgtgta
    52201 caagttgccg tctttatctg ctttatctgc agctactaaa acaacatttg gaatcaaatc
    52261 gatgaataag cgtccataaa gttccaaata agtatggata tcacctaatt tcattgtgcc
    52321 gtcttcaatc atttgggcga cacgcaagct ttgtgcacca gcataagaga aatcgatctt
    52381 gcttgcgatt cctttttcaa aaatattcaa atgttctggt cgtgagatac tagacatgat
    52441 caaatgcaga tcattcactt tttctgcatc tactttttca agactttctg aaagaaaact
    52501 tgcttgtttt tgattgtcgc cttctaatac aactttgtcg ccgggagcaa tcagtttttc
    52561 cattacttct acgatttttt cagtttctac atacttgcca ttgagcaagt tgctgatttt
    52621 ttccaatcgc tgcgcttttt tttcgcgttt agaagcccag ttgcggtttg gtttttccat
    52681 aattagtcat ctccattttt gatatagaaa cgtggcgtca tcaataccat gttggtctag
    52741 attgatcgta cggatccctt tatctacacc aaattttgta tgaatcagct ttgtcaaagt
    52801 attgccgggg ggaaattcaa tcgtcagatc cattcctaat tcttttgcca agtccatcat
    52861 ttggttccac tgcacaggga aagctaggtt ttccaacaaa tctttacgaa tctcttctgc
    52921 gtcctgagtg gctcttccat ccgtattttt tatatacaaa catctagcat cagatagatg
    52981 gacagactgc atatacggct tgaatttctt gaccgtatca ttcatcaaga gacagtgaga
    53041 tggcacaggg actttcagca gcttcgctgt ttgcgcttgg tgccgtttgg cttctgccaa
    53101 cgtaacggat aaaccttcga tactcccaga taacgtatgt tgcatcggac aattttcatt
    53161 tgacagataa actggatggt caggatcaaa tgtttttgct acaatttcct cagcttcgct
    53221 tctcgtcaca cctactacta cgcccattgc ataaccgctt ggataagctt ctttcattaa
    53281 gactgcgcgg tgatagacta gttccgctgc attctcaaat ctcaaagatc gacaagcaac
    53341 agctgcacaa aaagcaccaa tcgaatgtcc tgctgcaata tcaggctgaa taccgttttt
    53401 ttccatttca tctaaataaa atactgcttt tgtcagtaat gctagctgga taaaaaccgt
    53461 atcttgatag ttttcttctt tatcaactaa ccgatacccc gtcacatttt ctaaacgttc
    53521 catgtacatt tcagggatat ccgctaacat ccctaacttc tgactgcctt gtccaggaaa
    53581 taagaaacct gtactcatga gcctcgcctc ctcagcactt actttttcca ctgctatctt
    53641 agtcaatttc tgctataata aaaaatgcca aaaaaagaaa aaaccataac aaaaaggtat
    53701 ggatgggatg gataattatg aaattgcttc atctgcagta ttttaaaaaa gtattcgaaa
    53761 caaaaaatgt tacccaagca gccaaagaat tatttatttc tcaacctgcc ttgagccgtg
    53821 cgatcaaaca tttggaaaac gaattaggtg tccctttgtt ttaccatagc ggcagaaaca
    53881 ttgagacgac cgtttatgca gaagaatttt atccatatgc agtcaagaca ttagacacat
    53941 tagaagaagg aatcaaatta ttgtcttcgg tcaatgagaa gatcgttact tccgtcatat
    54001 tgtatttaga ggtagcctct gtttctattc cgaacttggt ccgaattttt atggaaaagc
    54061 atcctgatat ccaattaacg atcatgcaac atcatttacc agatgatatt accgatcctg
    54121 tactgtacat cacttcggag atgaagcctg gcatgaccaa tattcctatc atgaaagaac
    54181 ctgtttatgt cgccattcct aagaaacatc ctcttgctaa aaaagaacat ctgcaattag
    54241 aagatatcca acgcaccccc atcttgatgc tgtcaaagag caacgccttc cgtcatacac
    54301 tggatgcagc acttgaagcg aaaagtatcg aattgacgat cagttcgacg acagatgatc
    54361 ctgcgacatt acgttctatt gtcaagcaag gattagctgt tagctttttc ccaaaagttt
    54421 cttggtctta cgataaaaat gatcctttcg tcttgcggga aatcaaagat ttgcccttga
    54481 cacgaacaat ttatttatct tctagcttca cagaagatca tcctttgatc aaaatgattg
    54541 caaaaacgat gcgggaattt tatttaggaa aataaaaaaa aaagagctgg gacaaaattc
    54601 ttgtcgcagc tcttttttcg aacaaacggt gttccagaag taatttctta gaaaataagc
    54661 cgaaatttcc caaaaatttg agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct
    54721 aaacatttct gtcacaacct cttctctatt gtcctagaca ttcattgcgt tgatttaaaa
    54781 cttccatcaa tcaaatctgc tagttcatct agcttttcta ttttacgtag atcaatagag
    54841 atccaatgtt ctttattcat gtgataggct ggaaaatacc cctcttttcc tcgcaaaatt
    54901 ccgccaagtt caggatcgac cttcaaatca agaatattga tgatcctatg gtcacttgca
    54961 tcatcataga ttttttcttc tggaacagac atcaggaccc caaaccattt tttgcttttt
    55021 tgatgtctga atacgcaata atcaggaaat tttttaaaaa tatattctgg agacaaattg
    55081 tatgtttcat tgatgtgatc gattagttct tttttcgtta gcatctgctt ccatctcttt
    55141 cttattgatt cgctttattc aaagatacca cgtaagaata gaaaaatgaa tacaagtagt
    55201 tgctaatcaa atacttcgat atttttcttt tttatcaact cttaaatgtt caatccgcct
    55261 gtgttggtct tgctaccatc tactattaaa atgtttatta ttgtttgccc acctattttt
    55321 tgattttagt ggtatcattt ttcaagttca tgatgaaatt cttatcatca gtaaatgctc
    55381 cagaaaaata aacaatgatt gaaaaatcag ttgaaacttg accaccgttt attttctccg
    55441 tattcttaga atagaaggga gtctgattga tgtattcact aatgaaaaaa atcattaccg
    55501 aaaaagatat tcgacgtcat gtttctttag tagaacaatt gctgaatcat acaaagataa
    55561 ccgtaaatga attagctgaa atcataggga caaccgaacg aacgattttt tctgaccttc
    55621 aatctattcg ctctcagctg cccgaaggct gggacatcat cagcgatcaa gcaggaatca
    55681 gtttgcaaaa ccagcaaaat ttattaacaa atgacctttg ggagattttt ttcaaacaat
    55741 cagtcagtgt cgaactgctg aaaaatctgc tttttacaaa aaaagttgct gtccctgatt
    55801 ttctagccga ccatgggctt tcttatggga cattgaaaag acatgtgact aaaatcaatc
    55861 agcgtttagc aagttacgac ttgcagatcg atttgacgaa atatacagct tgtattcttg
    55921 gaaaagagcg tgcgattcgt actttttatc atcgattgct gattcctttc actcataaca
    55981 actatttttt cgaggattat tcgatccatg aatcccatta ttttcaattt ttaagaaacc
    56041 tcagtcaaac tgaactagcg gttgaaacag aagagatttt cggaacatgt tggttcttta
    56101 tcaatacgat ccgtataaaa gcgaattgcc gattagattc ttctatccat accaacagca
    56161 cactctcctc cctgtatgac tctgcattga aaaaattgta tttaacggaa ggtatttatc
    56221 ttaaagacac tgagctttct tttgcttctt tctgctttct ggaaagttgg aattacaaca
    56281 ataactatgg acaagaaacc gctcgctgcc tgcatcattc tccattttta gaggtcctcg
    56341 aaacgtttgt agaggaacta gcttcagagc tgtctcttga tcaactaaaa aaaacatctc
    56401 tgactgataa cttggctctc ctcatattaa aatatcatga atcgccgata ttgattgaag
    56461 cattggaccg tcaataccat cgttttttag aaacatatga tcagcaatat gcacacttgt
    56521 atcaacagaa agagaattta ctgaacgatc taaaaaaaga aatagaaatc aatgaaccaa
    56581 attactttct ccaactcttg tctctactga tccaacaaac gattttttct atcaaaccga
    56641 atttgttcaa tgtctatttt attttccaag gagaaccttc atggaaggcg ttcttgcagc
    56701 aggaattaaa agattactta ggaaaacgtg tctgcttctt acctatcgaa ctaaccgatc
    56761 tttccactat ttcttttgaa aaaacagata tcttgatttc caatttccct ctagattctc
    56821 tagatatccc tattgtatac atttcttcta ttccaacgaa aaatgagctc aatcgtttga
    56881 ctgaactcac tttcaaaact tttttatagt aacggctagc gactgtattc tctagctgtt
    56941 ttttcttttt tgcgaaacag tttgttcatg atcccttttt ctatttctac aactaagaat
    57001 agaagaatca catttgctcc aatcaataac tgttgtgtca tcgttaaact tactgtaccg
    57061 atcagctgct ggccgactgg tagaaaaata accatccctt gcaataaagc aaggatacct
    57121 aatgaaagaa aaagcgcttt gttttgcaaa agaccttggt tcaatgacgg cttggacaac
    57181 tctctgcaat tgatcatgta aacagcttga gctaagacga tattttgcag aagaatggtt
    57241 tgctgtaagc tttgcccttc aaactgcata gccaataaat aggcaggaac catgatcagt
    57301 agggagacat agatgatccg aaacaaatga taaccagata ggattccttg ctttggatct
    57361 cttggtgggc gcttcatgat ttctggatct ggcgactcga accctagtgc ataagataaa
    57421 gtgattgtcg ttaccatatt gacccataaa atctgtactg gggacaaagg tagcgggcga
    57481 ttcagcataa gtgcccaaat caagatcaat ccttgagcta atgcagttgg caagaagaaa
    57541 ttgatggttt tttgcaaatt atccaaaatc cgccggccct cttttacggc ttttgcaatc
    57601 gtatggaaat tatcatctcc taaaaccata tcagctgcct gtttgcttac ttcactacct
    57661 ttgattccca tagcaattcc tacatcggat cttttcaaag caggtgcatc atttacgccg
    57721 tctccggtca ttccgacgat ttctccattt ttctgcaaag ctttaacaat tcgcagttta
    57781 tgttctggtg tcgttcgagc aaagacatcg actattggca cttgctgaat caactcttca
    57841 tcagacataa gatccaaatc gatcccttct aaaactttgg cagtatgttt taagccaatt
    57901 tgttccgcaa tcgcttttgc tgtgtctttg tgatcccctg taatcatttt cactgagatc
    57961 cctgcttgtt ggcattcctt tacagctttg actgcacttt cttttggcgg atcgatcaat
    58021 ccggcgatcc ctgcaaaagt taattgggtt aaagtctcat gcgtgagttc ctgtttcgaa
    58081 tctacttttt tataagcaaa tcctaaaaca cgctgacctt ttctcgcaaa ttctgcagct
    58141 gtctgactcc atgcttcttg ttctgcagaa gaaagagttg atttagcaaa taatacttct
    58201 ggtgcacctt tgacgtaaat caccgctcct ttttcctccg catgcatcgt tgccatatat
    58261 ttgtattcag aactgaacgg aatctttgct tctatttttt tcaaaggtag attggctgtt
    58321 tctgtgtgtc gaatcagcgc aacttctgtc ggattgccac ttaaattttc tgttttctgt
    58381 tcttcttttg tttttaattc ctgacagttt ttcataatgg acaacacgca ggcttcttct
    58441 tttgcagcta catccacaac tgtcatttca ttttttgtca atgttcctgt cttatctgaa
    58501 cagatcactg tcatagaacc taatgtttct accgaaggaa gtcctttgat gatcgccttt
    58561 tcttttgcca tttcatgtac tccaacggat agaatcatgg tcaaaacagc tggcaatcct
    58621 tcgggtacca ttgacaccac caaagcaatc gtagccgaaa gaagaatatg ccaatccata
    58681 ccatatcgaa atgtcgtaaa aataactaag aataggctca aacaaagaat gccttgaaaa
    58741 atttgtttgt ttaatcggtt gattttttta atcagaggcg tcgtttgttg ttcgaccgat
    58801 tgcaatgctt gattgatttt accgatttct gtcgtgtttc ctgtttctac aacgacaccg
    58861 actgcagaac cagattgtac aagcgttcct gaaaaaccca tatttttctg gtcgccgatc
    58921 gattcttctt ctgtcaaaac ggcatgactt ttttcagatg cttctgattc tcccgtcaaa
    58981 atcgattctt ccactaataa atcatgtgct tcgattaatc ggatatctgc tggaacgaca
    59041 tctccggcac gtaagacaat gacatctcct tttaccagtt ctttcgcagg gattttttct
    59101 tgcatcccgt ttgatagtac tatcgcttct tgtcccatca gctgcttcaa accattcaat
    59161 gattcttccg cttttctttc ttgccagtaa ctaacgagtc cattgacgat cactacgaat
    59221 aaaataatac ttccttcaat atattcacta gaagcaaatt tcaaaaaaga ggcaatgatc
    59281 aacacgatca tcagcagatc agtaaaatgc tttgctattt ttttccatcg ttttgtctgc
    59341 tgccttgctt ttatttcatt atatccttga tcagtcaggc gtttctgtcg ttcttcttgc
    59401 gacaagcctt ctgttgatgt ttgatagttt ttcaatacat cttctaccgt caatctgtac
    59461 cagttcatcc tatcccctcc tgtttaattg atactgttat tatacgcagg gaagtttttt
    59521 caggcgtatc gattatttca ggggggtctg aaataattcg ttttgcaaaa tgtatctggg
    59581 cctaactgat atttctattg cttattcttt tatatacaaa atttaagaat acaaaccaga
    59641 taaccaataa aaaaggaata aagcgtgtcg tgctttcgaa taacaagctg aaagcaattg
    59701 aagtaaaaaa caccaacaaa gcaagatctt tatatctttg tgtagtgttc cgtttctttt
    59761 ctttaataaa ttgaacatgt gcacacaaga tcgaagacca cacaaataga aaacaactcg
    59821 ttgtcatgcc tgccaaaagc tgaaaaaaca atcgactatc agctacaaaa aaatgtagac
    59881 aaagaccggc aaaaaataac attcctgtaa ttaaaatacc gtgtaaaggt acgcctttct
    59941 tagaaagcgc acaaaaccaa tggggcaaac ttttttcttt tgctgtttga tataaaatcc
    60001 tgctagtact gtagactgca ctatttgctg aggacaaaga agcaaccaaa atcattagat
    60061 tgacaattgc tgctccgcct tggcaatgga tcacttgcag catagtcact aaaggacttt
    60121 cttttaaatc taaacaatgc catggaacaa caaataaaat cgacaaaatc gttcctatat
    60181 agaaaaagag aatacgccaa ggcagacagt tgattgcttt tttcaaattc ttttgcggat
    60241 cacttgtctc gccaacggtt aagccgatca tttcaattcc agcaaaactg aataaaacca
    60301 taggaaatga gactaccatt ccagcaaagc caaacggtag taattcatcg atctgtccaa
    60361 aattttcttt gattccgata gactgggtat gaaaatctga gacactcaat cctataccca
    60421 ataagataaa aaggaaaatc cccaagatct tgatgattga gaaaaaagat tccaattcac
    60481 caaaccactt gactgcaaca aggttgactg ccattaagat cattagtgcg ctacctccgc
    60541 ttatcaaagc tggtacatgt acaaaccaga aatggatata caatcctatt gctgttaatt
    60601 ccgcaagtcc agctccaagc cagcaaagcc aataagtcca attgacaaaa aatgcccact
    60661 ttttgcctaa ataatagtca acgaaatcac tgatagagtt aaatgtacta tttgctaaaa
    60721 ataaatcacc catggcccgc atcaaaccat aaatagccaa accgcatatc aaataaacga
    60781 gaataacagc tggccctgct gatgcaattg cctttgctga acctaaaaat aatcctgttc
    60841 caattgcgcc acctaatgcg attaactgga tatttcgatt agttagagtc cgtttcattg
    60901 ccggtttttc catcaagttc acctttttct ttttcaagca gcatggcgac ttcctgttcc
    60961 caagaaggtt tctttatttt ttcaatacag agtggtacta caaacaaaat gacactgatg
    61021 gctgtgactg caattagatt gatccatgtt ccagctttca tcgtcagtcc gacagaaaat
    61081 ccgattgccg caaataaaac aataacgaca gtccaaactc caagattgct ctttttctct
    61141 ttttttccaa ttctaaatgg tcgattcaac tttgcatgtg tataacgcaa tctcaaaact
    61201 gcgatcccca tgatgacata gacgatacag tagataacag tagttgaatt tacgagcatc
    61261 aaaaatgctt gattgacgcc aggaatcagc aaataaaaaa gtgcaaagat tgagataatg
    61321 acagactgag tcaaaataac ggatttggat acgtcaaatt gattcgtctt ccaaaaattg
    61381 aattttggtg gatacgcacc ttttctagca ctttctgtca ctgtctttgc tggtcctgag
    61441 gcccaagcag ataattggac tgctacacca atgaagacaa gtaaactaaa cagattcaca
    61501 ataagatgtg gcaaaccaag aatttggcaa taaatcgaaa tagattgcgc gatattattc
    61561 aattccattt gtccttttgg tacgacattt gctacaacta aagcattcaa aatattgacg
    61621 aaaaatataa aaattaacgc ggcaaatact ccgcgaatat acgttttaac tggattttcc
    61681 aatcgagtga tatacacaga ggacatttcg ataccagtaa aaataaacat gatcggcgca
    61741 aaataaacaa atgaggatgc agtcgtagta tctggtatta atttgtccca tgaaaaactg
    61801 cctaatgttc cggttggtac gatcccaact ttgatccacg cagcaaaccc taataagagc
    61861 atcatcgtca aaggcacgta caatcctaac caaacaccga cttttccgcc gatttttgcc
    61921 atatcaaatt tcaaattcaa aaaagtgatg atccaataaa cgactaaaat gacaactaat
    61981 gtaaatttgc tgttcaaacc aagcggcaca ttatcgataa tattaccaaa caacggagcg
    62041 agtgctgaag cgaccatcac catcccagga aacatttgga cccataacag ccatgagaca
    62101 acgaaccccc atttttttcc tagagcattc gatacccaaa gttctggacc acctttttgg
    62161 ggaaacattc cagcaaattc accagagatc aatgagattg gaaaagcgta caataatact
    62221 gcaaccagca tgtaaaaaat catcgtccag ctcgttgctg ctacatctgg aatatttcgt
    62281 acacttgcta caagggcgca ggtcattccg ataaaggtca tggtcgtgat ttgctttgat
    62341 gaaccagtat ctttcatctt tattccttct ttcaatagcg acatatgttt ttgttttctt
    62401 ttatttttgt tcaagaacag aaatgacaag gtttcatcga ttcagtacaa aacccagtat
    62461 caccgaaaca tccattgaac gatcttagtg aactgggttt gatacatgaa acgaacgatc
    62521 caacttatag aacgttttat aaaaaagaat atcttggcga ggaatatatg aaaaacaggg
    62581 ggaaatacaa ttgttttgat gaagcaaata attgtctgtt ctccatatgt ctcttgttat
    62641 tctttcacac aaaaatcagt tctattggat gatttcgttt agtttctttt ccattgcttt
    62701 ggcgattttt tttgtataaa aattgaaaat tcgatcatca ttcaaataag gagtcatgat
    62761 agccgcacgt agcagagtga ttttctgttc ttttttccat tcttctttcg ttagacccat
    62821 tctttcaaca aaacgaatcg gagaatcacc gtaatcttct tgtgtaaatg tagtatgaga
    62881 agtaataaac cgttcaccat aaacatcgcc atccatataa gaagaccgat cgaacatttt
    62941 ttcatttaat tcattgatag cgttcaaatc tgtacagcct tgttctttga atacccaatt
    63001 gaccatatta aaatcaggat gattcaatgg atagacttcg attgtttttc ctttaacagt
    63061 aaaagtcaga tgatcgagaa attctctgaa tctttgagct gcctcgattg aagccccaat
    63121 caattgacca tatccggtca cattcaatgg caatacacga tgtgctgtcc agactgcggc
    63181 ggcggttgct ccagcttttg atccttccaa aatataagct cctaacatat ctggagcctt
    63241 tactgatttt tcaaacacat atggcgcgaa ataagaaatg atatttctca tatttttatg
    63301 tttgattacg attcctccag cagcataagg gacatagccc atcttatgag gatctatcgt
    63361 gacagaatcc gcttctgaaa tagctctgaa cccttcatat acctctttgt caatcgttac
    63421 gcagtggtgg aaaacatgat gttcttcaaa gaattcagct aaggaagcat acggcacaaa
    63481 ttctcctgct tcgtttagaa acagtgaacg cgcatagccg ccatatgcgg catcaacatg
    63541 taaatagaaa tagatccctt catcttttaa cctttctctt aattggacga ttttatctac
    63601 actgtctact tgaccttctt ctgtcgttcc tacgacagca accacaccta gaattgggat
    63661 tttttgtcca gctaattcac gaattgtttt ttccaaagca ttaatatcca ttcgataatc
    63721 ctcttgaact gggatagcaa ccatctgatc taagccgact ccgcaaatat ctaacgcctt
    63781 catccaagaa tagtgttttg tttgagggac cagccatttc cctaatcttt ggatattttt
    63841 tccgctacgg gaagaagctg ctttgacctc atccatttct tcgtccgtaa agctttcggt
    63901 catttccaaa atttcttcaa cagataaatt caaaagttcc cactcagaca tttttttcac
    63961 tttttcaggc agcacttcct tgacagctaa aggaatcgat ttgatgcaac gggcgtacca
    64021 taatccttca agattagcaa tagaaccatc tgctgcaatg tgtccccatc cgtctgtcat
    64081 attgaataat gaagcaaaat cctgtcctac ttctgcttcc atttgcgaag ttgccataga
    64141 agattccaat gcaacattat ttgggttcca cagcatcgca tagttgtaag ctaataaagc
    64201 aggcatcaat gtttcagcat tcatttgtcc ccagtaacgg cctgctgaat gccatgggat
    64261 cgatcctgcg cgtaatcgct gactgacttc atccaagacc tctatcatat ggtcgcgggt
    64321 ggctagataa tctggagtga gcttatctcc ttcgctaatt gcaggcatat ctgaaggaat
    64381 ataattttct ctccatccaa gatgttcatc taccatcttg ttcaacaaca ttttataaac
    64441 tggtccattt tcagctttgt cgcctataaa gacggccttg atatccatat ctttcatttt
    64501 tcttccctcc atattttgtt ggtcaatgtc ttgcagatac atgtgtttat ctgtaacacc
    64561 tcttcactat agcgccaaaa atgagaattt tttttttata acatcaaatc cacgtagatt
    64621 tttacaaaca aaaataaata a
//
LOCUS       DAYYGO010000003        61712 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000003 DAYYGO010000000
VERSION     DAYYGO010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.003"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(132..509)
                     /locus_tag="ACHA5T_000136"
     CDS             complement(132..509)
                     /locus_tag="ACHA5T_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8885746.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            765..1493
                     /gene="purC"
                     /locus_tag="ACHA5T_000137"
     CDS             765..1493
                     /gene="purC"
                     /locus_tag="ACHA5T_000137"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFM8885747.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1493..1747
                     /gene="purS"
                     /locus_tag="ACHA5T_000138"
     CDS             1493..1747
                     /gene="purS"
                     /locus_tag="ACHA5T_000138"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFM8885748.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1749..2420
                     /gene="purQ"
                     /locus_tag="ACHA5T_000139"
     CDS             1749..2420
                     /gene="purQ"
                     /locus_tag="ACHA5T_000139"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFM8885749.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2421..4643
                     /gene="purL"
                     /locus_tag="ACHA5T_000140"
     CDS             2421..4643
                     /gene="purL"
                     /locus_tag="ACHA5T_000140"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFM8885750.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4628..6067
                     /gene="purF"
                     /locus_tag="ACHA5T_000141"
     CDS             4628..6067
                     /gene="purF"
                     /locus_tag="ACHA5T_000141"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFM8885751.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6090..7142
                     /gene="purM"
                     /locus_tag="ACHA5T_000142"
     CDS             6090..7142
                     /gene="purM"
                     /locus_tag="ACHA5T_000142"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFM8885752.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            7139..7717
                     /gene="purN"
                     /locus_tag="ACHA5T_000143"
     CDS             7139..7717
                     /gene="purN"
                     /locus_tag="ACHA5T_000143"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFM8885753.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7714..9249
                     /gene="purH"
                     /locus_tag="ACHA5T_000144"
     CDS             7714..9249
                     /gene="purH"
                     /locus_tag="ACHA5T_000144"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFM8885754.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9265..10539
                     /gene="purD"
                     /locus_tag="ACHA5T_000145"
     CDS             9265..10539
                     /gene="purD"
                     /locus_tag="ACHA5T_000145"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFM8885755.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10661..11575
                     /locus_tag="ACHA5T_000146"
     CDS             10661..11575
                     /locus_tag="ACHA5T_000146"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFM8885756.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11708..13273)
                     /locus_tag="ACHA5T_000147"
     CDS             complement(11708..13273)
                     /locus_tag="ACHA5T_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8885757.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13266..14717)
                     /locus_tag="ACHA5T_000148"
     CDS             complement(13266..14717)
                     /locus_tag="ACHA5T_000148"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8885758.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14731..15747)
                     /locus_tag="ACHA5T_000149"
     CDS             complement(14731..15747)
                     /locus_tag="ACHA5T_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8885759.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15967..17055
                     /gene="chvE"
                     /locus_tag="ACHA5T_000150"
     CDS             15967..17055
                     /gene="chvE"
                     /locus_tag="ACHA5T_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM8885760.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17109..18635
                     /gene="mmsA"
                     /locus_tag="ACHA5T_000151"
     CDS             17109..18635
                     /gene="mmsA"
                     /locus_tag="ACHA5T_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8885761.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18637..19842
                     /gene="mmsB"
                     /locus_tag="ACHA5T_000152"
     CDS             18637..19842
                     /gene="mmsB"
                     /locus_tag="ACHA5T_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFM8885762.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20099..21076
                     /gene="guaC"
                     /locus_tag="ACHA5T_000153"
     CDS             20099..21076
                     /gene="guaC"
                     /locus_tag="ACHA5T_000153"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFM8885763.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21340..22764
                     /locus_tag="ACHA5T_000154"
     CDS             21340..22764
                     /locus_tag="ACHA5T_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFM8885764.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(22806..23063)
                     /locus_tag="ACHA5T_000155"
     CDS             complement(22806..23063)
                     /locus_tag="ACHA5T_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8885765.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            23225..24334
                     /gene="pdhA"
                     /locus_tag="ACHA5T_000156"
     CDS             23225..24334
                     /gene="pdhA"
                     /locus_tag="ACHA5T_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFM8885766.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24337..25314
                     /locus_tag="ACHA5T_000157"
     CDS             24337..25314
                     /locus_tag="ACHA5T_000157"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFM8885767.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            25339..26982
                     /locus_tag="ACHA5T_000158"
     CDS             25339..26982
                     /locus_tag="ACHA5T_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFM8885768.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            26990..28396
                     /gene="lpdA"
                     /locus_tag="ACHA5T_000159"
     CDS             26990..28396
                     /gene="lpdA"
                     /locus_tag="ACHA5T_000159"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFM8885769.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            28558..28833
                     /locus_tag="ACHA5T_000160"
     CDS             28558..28833
                     /locus_tag="ACHA5T_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFM8885770.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            28839..29606
                     /locus_tag="ACHA5T_000161"
     CDS             28839..29606
                     /locus_tag="ACHA5T_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFM8885771.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            29774..31603
                     /gene="typA"
                     /locus_tag="ACHA5T_000162"
     CDS             29774..31603
                     /gene="typA"
                     /locus_tag="ACHA5T_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFM8885772.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            31864..33636
                     /gene="recQ"
                     /locus_tag="ACHA5T_000163"
     CDS             31864..33636
                     /gene="recQ"
                     /locus_tag="ACHA5T_000163"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFM8885773.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            33750..34052
                     /locus_tag="ACHA5T_000164"
     CDS             33750..34052
                     /locus_tag="ACHA5T_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFM8885774.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            34055..35218
                     /locus_tag="ACHA5T_000165"
     CDS             34055..35218
                     /locus_tag="ACHA5T_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFM8885775.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            35244..38672
                     /locus_tag="ACHA5T_000166"
     CDS             35244..38672
                     /locus_tag="ACHA5T_000166"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFM8885776.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            38693..39817
                     /locus_tag="ACHA5T_000167"
     CDS             38693..39817
                     /locus_tag="ACHA5T_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFM8885777.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            39798..40244
                     /locus_tag="ACHA5T_000168"
     CDS             39798..40244
                     /locus_tag="ACHA5T_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM8885778.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            40228..40566
                     /locus_tag="ACHA5T_000169"
     CDS             40228..40566
                     /locus_tag="ACHA5T_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFM8885779.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            40685..41245
                     /gene="rsmD"
                     /locus_tag="ACHA5T_000170"
     CDS             40685..41245
                     /gene="rsmD"
                     /locus_tag="ACHA5T_000170"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFM8885780.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            41242..41733
                     /gene="coaD"
                     /locus_tag="ACHA5T_000171"
     CDS             41242..41733
                     /gene="coaD"
                     /locus_tag="ACHA5T_000171"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFM8885781.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            41723..42766
                     /locus_tag="ACHA5T_000172"
     CDS             41723..42766
                     /locus_tag="ACHA5T_000172"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFM8885782.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            42925..43653
                     /locus_tag="ACHA5T_000173"
     CDS             42925..43653
                     /locus_tag="ACHA5T_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFM8885783.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            43769..44014
                     /locus_tag="ACHA5T_000174"
     CDS             43769..44014
                     /locus_tag="ACHA5T_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM8885784.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            44011..44229
                     /locus_tag="ACHA5T_000175"
     CDS             44011..44229
                     /locus_tag="ACHA5T_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFM8885785.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            44365..44904
                     /locus_tag="ACHA5T_000176"
     CDS             44365..44904
                     /locus_tag="ACHA5T_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFM8885786.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            44897..47170
                     /locus_tag="ACHA5T_000177"
     CDS             44897..47170
                     /locus_tag="ACHA5T_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFM8885787.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            47208..48254
                     /gene="holA"
                     /locus_tag="ACHA5T_000178"
     CDS             47208..48254
                     /gene="holA"
                     /locus_tag="ACHA5T_000178"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFM8885788.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(48343..48597)
                     /gene="rpsT"
                     /locus_tag="ACHA5T_000179"
     CDS             complement(48343..48597)
                     /gene="rpsT"
                     /locus_tag="ACHA5T_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFM8885789.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(48997..49266)
                     /locus_tag="ACHA5T_000180"
     CDS             complement(48997..49266)
                     /locus_tag="ACHA5T_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8885790.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(49528..49656)
                     /locus_tag="ACHA5T_000181"
     CDS             complement(49528..49656)
                     /locus_tag="ACHA5T_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8885791.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            complement(49736..50191)
                     /locus_tag="ACHA5T_000182"
     CDS             complement(49736..50191)
                     /locus_tag="ACHA5T_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFM8885792.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(50329..52257)
                     /locus_tag="ACHA5T_000183"
     CDS             complement(50329..52257)
                     /locus_tag="ACHA5T_000183"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8885793.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(52454..53287)
                     /locus_tag="ACHA5T_000184"
     CDS             complement(52454..53287)
                     /locus_tag="ACHA5T_000184"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFM8885794.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(53479..54183)
                     /locus_tag="ACHA5T_000185"
     CDS             complement(53479..54183)
                     /locus_tag="ACHA5T_000185"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8885795.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(54206..54832)
                     /locus_tag="ACHA5T_000186"
     CDS             complement(54206..54832)
                     /locus_tag="ACHA5T_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8885796.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            55158..57533
                     /locus_tag="ACHA5T_000187"
     CDS             55158..57533
                     /locus_tag="ACHA5T_000187"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFM8885797.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(57557..58048)
                     /locus_tag="ACHA5T_000188"
     CDS             complement(57557..58048)
                     /locus_tag="ACHA5T_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFM8885798.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            58235..59155
                     /locus_tag="ACHA5T_000189"
     CDS             58235..59155
                     /locus_tag="ACHA5T_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8885799.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            59309..59653
                     /locus_tag="ACHA5T_000190"
     CDS             59309..59653
                     /locus_tag="ACHA5T_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFM8885800.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(59843..60343)
                     /locus_tag="ACHA5T_000191"
     CDS             complement(59843..60343)
                     /locus_tag="ACHA5T_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8885801.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            60871..60954
                     /locus_tag="ACHA5T_000192"
     tRNA            60871..60954
                     /locus_tag="ACHA5T_000192"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:60905..60907,aa:Leu,seq:cag)
     gene            complement(61278..>61712)
                     /locus_tag="ACHA5T_000193"
     CDS             complement(61278..>61712)
                     /locus_tag="ACHA5T_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFM8885802.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        20241 a        10663 c        13217 g        17591 t
ORIGIN      
        1 caaaaaagaa gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc
       61 aataatagga acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa
      121 aaatagagga attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga
      181 tgttgcagca agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg
      241 atacgataat gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct
      301 ttgtgttctg tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct
      361 cggcggattt ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg
      421 atcgacagca aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa
      481 agctgacctt gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa
      541 atttttagtt aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta
      601 taagattttc gtttctgata aaacacgaac attaaaagaa tctaatgcct taaatgtaca
      661 tatttttgtt gatttataaa aagatgattg ctaaaattta aagtgaaaaa acgaacaaaa
      721 aacgatgtat ttgcttttaa tgtaaataaa aaggagagac gtacatggag aagaaagaac
      781 ttttgtatga aggaaaagcc aaaaaaatat atcttacttc tgatgagaac ttgatttgga
      841 tagaatattt agatcaagca actgcgttga atggcgcgag gaaagaccag ataagtggaa
      901 aaggagaatt gaataatcaa atcacaagtg caattttccg ttatctgact caaagaggaa
      961 taaaaaatca ttttattgaa caattgtcta aaaccgaaca attagcaaaa aaagtacaaa
     1021 ttatccctct ggaagtggtt atccgaaata ttgcagcagg aagtttctca aaacgtctag
     1081 gtgttcccga aggtaaagaa ttcatcaaac caatcattga attctactat aaggatgacc
     1141 agttggatga tccatttatc aatgaagagc atatctattt cttacaactt gcaaagccgg
     1201 aagaaatcag tgagctgaaa gcccaagcgt tggctattaa tgaaattttg accgaattat
     1261 ttgctaaggt cggtttggtt ttagtagatt tcaagctaga atttggccgg tcgaatggac
     1321 aaattttgct tgcggatgaa gtaacaccgg atacttgccg attatgggat gaacgtacga
     1381 atgaacatat ggacaaagac gtttaccgca gagatctagg tgatttgatc ccagtctatc
     1441 aagaagtata taaccgtcta tcggaactaa ctgaagaaaa ggagcgagta tgatgtattt
     1501 cgtaaaagta tatgtaacct ataaagattc agtgttagat cctcaagggg aggcagtaaa
     1561 aaaagcagtc catcgtatgg gatatgaaac aatcgaagat atccgaatag gaaaatactt
     1621 tgagatacaa cttgctgatg cagaagaacc aatcgataca gtgattgaaa cgatttgtga
     1681 caagctgctt gcaaatgtaa atatggaaac ataccgatat gaaatcaaaa aaatggagga
     1741 agtataacat gaaatttgcg gtcatcgttt ttccaggttc aaattgtgac gcagatatgt
     1801 tatgggcagt tcgcgagatc atgggcgctg atgccgaatt tgtacggcat gatgagtcct
     1861 ctttagaagg atttgatggg gttttactgc cgggaggatt ctcctatggt gactatttga
     1921 gatgcggtgc gattgctcgg ttttctacaa tcatggaaga agtgattcgt tttgctgaag
     1981 aagggaaacc agtattcggt acatgcaatg gattccagat attgacagaa gcaggacttt
     2041 tgccaggtgt actgagacga aatgagtctt tgcattttat ttgcaaaact gtaccattga
     2101 aagtcaaccc caagacggcc tttacttcag agtatgaagc cgacgaaata atctatcttc
     2161 cagttgctca tggggaagga aattatttct gtgacgaaga aacacttcaa gaactaaaaa
     2221 aaaataatca gattgccttt acttataatg acaacatcaa cggcagcgtg gaaaacattg
     2281 cagggattac gaacaaagca gggaatgtgt taggaatgat gcctcatcca gaacgtgcag
     2341 tagaagactt gctaggatca gaagatggga aacggttctt tgcttccatt ttaaaaaact
     2401 tcggaaaggt gaccgtatag atgaaaacat tgaaagaacc gacagctgaa gagatcaaga
     2461 aaaaaagaat ctatgccgaa tggggattga ctgatgaaga atatcgaatg atcgaagaag
     2521 atatcttagg aagaatgcct aattatacgg aaactggact gttttctgtt atgtggagcg
     2581 aacattgttc ctataaaaat tctaaaccgg ttctacgcaa atttcctact agtggacctc
     2641 atgtgttgca aggccctgga gaaggcgcag gcatcgtcga catcggtgat ggactagcag
     2701 ttgtcttcaa agcagaaagt cacaatcatc cttcagccgt ggagccttat gaaggagctg
     2761 cgacaggagt tggcggaatc atcagagata ttttcagcat gggggctcgt ccaattgcgt
     2821 tgctagattc attgaggttc ggcgaattag ataatccaag aacgaaatat ttactagaag
     2881 aagtagttgc aggtatcggc ggatacggca attgtatcgg cataccgact gtaggcggcg
     2941 aaattgcatt tgatccttgt tatgaaggaa acccattagt caatgcaatg tgcgtaggtt
     3001 tgattgaaca caaacatatc cagaaagggc aagccgctgg cgtcggtaat tcgatcatgt
     3061 atgtaggtgc aaaaacaggt cgcgacggta tccacggagc aacttttgcc tcagaagaat
     3121 ttaatcaaga agaagaacag caacgttctg ctgtacaagt aggcgatcct tttatggaaa
     3181 agctgctgct tgaagcgtgt ttggaattaa tcttgcatca ttcggattct ctaataggta
     3241 tacaggatat gggagcagca ggacttgttt catctagtgc ggaaatggct tcaaaagctg
     3301 gctcaggact tatattgaca ttagatgaag tcccacaaag agaaactggg atgacacctt
     3361 atgaaatgat gttatcggaa tcacaagaac ggatgttgat ttgtgttaaa tcaggagagg
     3421 aagaaaaaat ccaagaacta tttcagaagt atgatctaga tgcagtcact atcggtaaag
     3481 taacagacga cgggcaatat cggctatatc atcaaggaga agaagtagca aatctgccag
     3541 tcgatgcatt agctgaagat gcaccagttt atcataaaga aatgaaagaa ccagctcgaa
     3601 tcgtagaatt tcagcagtta gctccttatc agccagttat cgaagaacct ggacaagtat
     3661 tgcttgatct gctgcagcaa ccgacgattg cttcaaaacg ttcaatctac gaaacttatg
     3721 attcacaagt acaaaccaat acagttgtcc gtccaggaag cgatgctgct gtgatgaggg
     3781 ttagagggac gaataaagcg ttagctatga ccacagactg caacgcgcgc tatctgtatt
     3841 tggatccaaa aatcggagga caaatagcag tggcagaagc tgctagaaat attattgcta
     3901 gcggtggtaa gcccttggcg atcaccgact gcctgaacta tggttctcct gataaaccag
     3961 aagttttttg ggaattatcg acttcagctg acggaattgc tgctgcgtgt gaaacgttag
     4021 gaactcctgt tatttcagga aatgtctctt tatataatga aacggatggt caagcaatct
     4081 atccaacacc aatgattggt atggtcggat tgattgagga tcataaacat atcactactc
     4141 aggaattcaa aaaatcaggg gatctaatct atatattagg taagacgttt gcagattttg
     4201 atggcagtga attacaaaag atgcagcttg gaagaatcga aggtgtgatc cgaaactttg
     4261 atctatccat tgaaaagaga aaccaagagt tagtattgac agccatccaa aacggattga
     4321 ttgaaagtgc tcatgactgt tcagaaggtg gcttggcaat tgcactggca gaatcagctt
     4381 tcaaacatca gctagggata tctgtacagt ttgaattgtc ctctgctcaa ttatttgcag
     4441 aaactcaatc aagatttgtg ttgacagtcg caccggaaaa taagacacga ttcgaagaaa
     4501 tgatgggaga tgctgctgtt ttagcaggta aggtgacgga tgaagcgata atcgaaattt
     4561 cagcaactga cggtcagatc aaaatagaaa ctgcagtagc aagaaagtgc tgggaggatg
     4621 caatcgtatg tcttatgaag tgaagagttt aaatgaagaa tgcgggatct tcggtgtttg
     4681 gggtcatcca gacgccgcta gtgtggcata cttcgggctc catagtcttc agcatcgagg
     4741 acaagaaggt gcaggtatcg tgtccaatga tcgaggaaag cttaaggggc atcgagattt
     4801 ggggctgctt tctgaggtat tcaaagatca aagaaaattg cagcagttaa caggagaagc
     4861 tgcaatcgga catgtacgtt atgcaacagc aggcaatgga agtgtggaca atattcagcc
     4921 ttttctattc aaatttcatg atcagtcgat cggcttagcg cataatggta acttgatcaa
     4981 tgctcgttcc cttagaaaag agttagagga aaatggggcg atcttccatt ctaactccga
     5041 tacggagata ttgatgcatt tgatccgacg aagtgaagaa acagattttt taaataaatt
     5101 aaaaagtgct ttgaatgaag tcaaaggtgg ctttgcctat ttattaatga cagaaacggc
     5161 aatgattgcc gctttggacc caaatggttt tcgtccatta gcaatcggac aaatgaaaaa
     5221 cggagcatac gtgattgcct cggaaacttg tgcattagaa gttattgggg cacgttttat
     5281 aagagatgtc cagcctggtg agatagtgat catcaatgac gaaggaatcc agattgattg
     5341 ctttacgaag gacacgcagc tttctatttg ttcgatggaa tatatctatt ttgccagacc
     5401 agattcaaac atcgcaggga tcaatgtgca tactgcacgg aaaaatatgg gacggaattt
     5461 agcgaaagaa tcgccagtaa aagcagatat ggtcgttggc gtacctaatt cttctttgtc
     5521 agccgcaagc ggttatgcgg aagctagtgg aatcccttat gaaatcggat tagtaaaaaa
     5581 tcaatacatt gcccgaacat ttatccagcc aacgcctgag ctaagagagc aaggtgttcg
     5641 aatgaaatta tctgctgtac gaggagtagt ggaaggtaaa aaggtgatta tggtcgatga
     5701 ttcaattgtt cggggtacga caagcagacg gatcgtccat cttttgaaag aagcaggggc
     5761 aaaagaagtc catgtgagga ttgcttctcc tcctttgaaa tatccatgtt tttacgggat
     5821 tgatattcaa acgcgtaaag aactcattgc ggcaaatcac acgattgatg aaatcaaaga
     5881 atgtatcggt gcagattctt taggtttttt aagtgaagag ggtctgatcg aatcgatcgg
     5941 tctggaaaaa gatgctccgt attcaggatt gtgtatggct tatttcaacg gagattatcc
     6001 gacgccgtta tatgactatg aagaaaaata tcaggaatca ctaaaagaaa aagtatcgtt
     6061 cttttaaaag cagaccaaga agggagtata tgaattcaat ggcaaatgca tacgcaaaag
     6121 ctggcgtaga tgtcgaagct ggatatgaag tagtagaacg gatcaagaag catgtcaaaa
     6181 aaacggaacg tttaggtatg atgggcgcta taggaggatt cggcggttgt tttgatttat
     6241 cggtctttca attaaaagag ccagtccttg tctcggggac cgatggcgtg gggacgaaat
     6301 tagcagtggc aatcgaagca gacaaacacg atacaatcgg gatcgactgc gtagcgatgt
     6361 gtgtcaatga tatcttagca caaggcgcag agccgttata tttcttagac tacttagcaa
     6421 cggggaaaaa tgatccgtta cgtttggaaa atgtagtaaa aggtgtagca gatggatgtg
     6481 ttcaaggaaa tatggcgctg attggcggag aaacagctga aatgcctggt atgtactcag
     6541 gtaatgatta tgatctagcc ggatttgctg tagggatagc tgaaaaatct caattattga
     6601 caggagaaac gatcaaccca ggagatgtgt tgcttggaat agcatctagc gggattcatt
     6661 caaatgggta ctctcttgtt cgcaaaattt tctttgagca gcagcagttt tctttatcat
     6721 caaaactgcc agaattgaaa aaaacacttg gcgaagaatt gcttgagcca acgaaaatct
     6781 atgtttcttc cttgcttccg ctgatcaaag aaaagaaaat aaaaggagct gcacatatca
     6841 ctggtggagg gtttgtagaa aatatcccgc gaatgttacc agaatctatg gctgcacaaa
     6901 tcaaacttgg ctcatggccg attcttccaa tattcggctg tttacaaaaa tatggtgaaa
     6961 ttcctgagat ggagatgtat gaaatcttca atatgggcat cggtatggtc ctggcagtcc
     7021 aagaatcgaa agtaaaagaa atagtacacg aaattgagca agctggcgaa aaagtttatg
     7081 aaatcgggaa agtgatcgcc aaagaaaaac atcgtgtggt gtttatagga gaagaacgat
     7141 gagaatcgct gtttttgctt ctggaaacgg aagcaacttt caagcgctag ccgattatct
     7201 atcgaaaaaa ggattggaat cttcgattga ctggttgttt tgtgatcagc ctgaagcata
     7261 tgttttgaaa cgtgcaactg cgttgagtgt accggcagat tgtttttcac ctaaagaatt
     7321 tgattcaaaa aaagagtatg aagaagcaat tttgcataaa ttgaaagaaa aaaagatcga
     7381 tttgatcgta ttagctggat atatgcgaat cattggaccg gtattgctga aaaattatga
     7441 taaaagaatc atcaatatcc atccgtctct tttacctgcc ttccctggtt tgcatggcat
     7501 acgtgatgcc tttgaagctg gagtaaaaga aacgggtgtc acaattcatt atatcgacca
     7561 aggtgtagat actggaccaa tcatccggca ggaaaaagtg agaattgaac aggaggacac
     7621 gttcgattct ttagaagaaa aaatccatag agtagagcat cgaatctatc cagaagtgat
     7681 tagtgaaatt attgaaaatg gaggctatct gaaatgacaa gagcattgat cagtgtatca
     7741 gataaaactg ggattgtagc attcgcccaa ggtttgattg aacaaggagt agaaatcatt
     7801 tcaacaggag gaacaaagca tgcgttggat aaagctggga tcaaaacgct ttcgatcgaa
     7861 gaagtgacag ggtttcctga aatgatggat ggccgtgtca aaacacttca tcctaaaatc
     7921 catggtggtc ttttaggaag acgcgatctc cctagccata tggaggcaat ggaaacggaa
     7981 aatatccaac caatcgactt tgtttgtgtc aatttatatc ctttcaaaga aacgattatg
     8041 aaaccagatg tcgaaatagc agaagctatt gaaaacattg atatcggtgg tcctagtatg
     8101 ctccgtagtg cagcaaaaaa ccatgaattt gtcacctctg ttgttgatcc atctgactat
     8161 gaagaagtac tttcagaatt acgtcagtat gggcatactt cactttcttt cagacaaaaa
     8221 ctagcggcaa aagtctttcg tcatactgcg gcatatgatg cactgatcgc tcaatactta
     8281 acggattggg tggaagaaaa agaaccggaa aaattgacac ttacgtatga acgaaaacaa
     8341 acgcttcgtt acggtgaaaa tagccatcaa gccgctgcct tttatcagtc cgctcttctg
     8401 gaaaaatatt cgatcgcagc agcaaaacaa ctccatggga aagaactttc ttataataat
     8461 atccgagatg cagatgctgc gctacggatc atgaaggaat atgacgagcc aacggtagtc
     8521 gccttgaaac acatgaatcc ttgtggtatt gggacagcat ctacgatttt agaagcgtgg
     8581 gaagcagcgt atgaagcaga tcctgtttca atctttggtg ggatcatcgt tttgaatcga
     8641 gaagtagatc tgccaacagc agaacagatg cataagctgt ttctagaaat catcattgcc
     8701 ccttcttata cacaagaagc tttggaatta ctgcaaaaaa agaaaaacat ccgattactg
     8761 acagtagact ttgcgcagga agaaaatcaa aatcgagaag aaacggtctc agtcttaggt
     8821 ggacttcttg tacaagatca agatctggca atagaaaatg aagaagagtg gaaagtcgtg
     8881 acaaaacgta agccgactcc agcagaagca aaagcattag cgtttgcttg gagagctgtc
     8941 aaacatgtga aatctaatgc gatcgtactg gcaaacgaac gtcagacagt aggaatcgga
     9001 gccgggcaga tgaatcgagt cggttcagta aaaattgcga ttgaacaagc gcaagagaaa
     9061 atggaaggtg cagttttagc tagcgatgct tattttccaa tggatgattc ggttgaatat
     9121 gcagccaaac atggtataaa agcaatcatt cagccaggag gaagcatcaa agaccaagat
     9181 tctattgata tggcgaataa atacgatcta gcaatggtct ttactgatgt tcggcatttt
     9241 agacattaga gaggagtcgg ataaatgaac attttggtga tcggtgcagg tggtcgtgaa
     9301 catgcaataa gtaaaaaact tttagccagt ccgaaggtgg atacggtgta ttgtgcgcca
     9361 ggaaatccgg ggatgacaaa agatggtatc aattgtgtcg ctatcagtga gcgagatcat
     9421 tcagcgttaa tccattttgt taagaaagag gctattgatt ggagtatcgt tggtcctgaa
     9481 gttcctttgt taaatgggat cgtcgatgat tttatggcgg aaggattgaa aatcttcggt
     9541 ccgacaaaag cagcagctct gatcgaaggt tcgaaaagtt ttgctaaaca attaatggat
     9601 acttatcaaa tcccgacagc taaatccaga agttttgaat gttacgaaaa agcagcagcg
     9661 tatatcaaag aaaatggtgc gccaatcgta gtgaaagcgg acggcttagc agctgggaaa
     9721 ggtgtagtcg tggctaccac agtgaaggaa gcgctggatg cggcagaaca aatgttgaac
     9781 cagcatcgat ttggggatag tagcaaaaaa atcgtcgtag aagagttttt ggcaggagaa
     9841 gaattctccc ttttggcgtt tgtccgtaat accaaggtgt atccgatggt catttcgcaa
     9901 gatcataagc gggcatttga taacgataaa gggccaaata caggcggaat gggtgcttat
     9961 tcaccagtac cgcaaatttc aaaaaagatg attgaaaaag ctgtggaaga agttttaaaa
    10021 ccagcggctt tgggaatgaa agaactagga cgctctttca cgggtgtttt atatgcgggt
    10081 ttgattgcta cgaaggaagg accaaaagtg atcgaattca atgcgcgttt cggtgatcct
    10141 gaaacgcaag tcgttctttc tcggttgaag agtgactttg cgcaagtaat cgatgattta
    10201 ttagagaatc gtacagtaga gttaaagtgg cagcaagaag gatacaatgt aggcgttgtg
    10261 gttgcagctg ccggttaccc cgaagaatat gagacaggaa tggttctacc agattttctg
    10321 gaagaagagc tttctgttta ctatgctgga gtttcctcaa ataacggcaa actagttgga
    10381 gcaggaggac gtctttattt agtagaagca tacggtgaga cgatcaaaga agcacagaaa
    10441 aaaatttaca ccgcgttaat ggataaaacg acagatggaa cattttatcg tacagatatt
    10501 ggagaaaaag ctgccaatag cgaactgatc gtgaagtaaa tcagctgctt ccatttataa
    10561 aaaatacaac aaacataatc ggaagcgccg attttagtga agccttttca taaagagtgt
    10621 ggtacacttt tgttgtgtat ataaataagg aggagttcag atgaaaaaaa ttcaattagg
    10681 tacttctgat cagaaagcag ctgcagtgat cttaggctgt atgcgtatca acaatgcgga
    10741 agatccagta aaagtgattg agacagctta tgaaaacggc attgattttt ttgaccatgc
    10801 agatatttat ggaaacggtg aatgtgaaga aatctttgca gatgctctag caaaaacatc
    10861 aatcaaacga gaagatcttt ttattcaaac aaaatgtgga atcgttccag gtgtgatgtt
    10921 tgatttttct aaaaaacata tcattgaatc tgtagaaaaa agtttgaccc gtctgaaaat
    10981 ggattatgtg gatgctttac ttcttcatcg tccagataca ttggtagaac cagaggaagt
    11041 agctgaagcg tttgatgaat tggaacggtc aggaaaagtt cgttattttg gcgtcagcaa
    11101 tcaaaaacca atgcaaatcg aattgctgaa aaaatatgtt cgtcaaccat tagtagctaa
    11161 tcagctgcaa tttggcttga aacatactgg aatgatcgat caagggattc atgtcaatat
    11221 gacagatgaa gcaagtatcg atcatgacgg tagtgtactt gattattccc gattgcatga
    11281 tatgacgatc caagcatggt caccatacca gtatggattt tttgaaggtg tatttatcgg
    11341 gaacgaaaaa ttccctgaat taaaccaaac attagaagaa ctagcagcta aatatcaaac
    11401 gacaccaact ggcttggctt ctgcttggat tttacgccat ccagccaata tgcaagtgat
    11461 cgccggtacg atgaacgtag ggcgaatcaa ggaaatcgct caagcttcag aaattaatat
    11521 gtctcgggaa gattggtacg aattgtaccg tgctgctgga aacattttac cttaaagatt
    11581 gtcataagca ggtcaaaagg gctataaatt aaaaagacag aataacttga aagctgtttt
    11641 cagcttcgag atattctgtc tttttttatt gcaaatggaa agagtatcat tgtacggatt
    11701 tcgtttatta gttatttttg tttttaagga aaagctgtgt ccgatattct tttggagaaa
    11761 tcccttgttt tttcttaaat aaataactga aatagttagg gtcattataa ccaatttcaa
    11821 aagcaatatc tgaaatacga tagtctgatt gctgcaataa tttttttgct aattcgattc
    11881 tttgttctgt cagataatcg atgaatgttt ggttaaacga ttgagaaaaa atcgtactga
    11941 aatgagaggg acttaatgca acttgttgag cgacccaagt taaggacata tctggatcgg
    12001 taaaattttc tttgataaaa tccaatgcat ggttcaaaat tggctgatat ttgttccttg
    12061 aaggctgtga gggcagagca gttaagaaag aaatgaacat ataaactgtc gaacggtaaa
    12121 gcgagtcgtc ggcgctgatt gctaagcgtt gactggtgga tgtgcaatga accatttctt
    12181 gcgggatttt cttttctttc aggcagacat gctggagtag cttagtcaag acaaataaac
    12241 gatataatcg tgttcgagtt ggtgtgcctt gtggtttcgt caagtgttcg atgtacttag
    12301 atacttcgtt tttcgataat gaagacaaat caagtgagag gtcaaagtaa aagacagaat
    12361 caactggaag tttcgcttct tgaatcaaat cctcatagct gataatttgt tctttaaggg
    12421 aactggcggt ttcaagtatc atctgcgtga tctcaaaaga tgcaggtaat tcgctgattc
    12481 tttctgcgac aggaccaatg gcgacagaaa aattattttt ttgtcccttt tctaattcat
    12541 ggaccaacgt ttgggctacc tgataactgg tctcgatcac attttcttca agagaatcaa
    12601 ctattaaaaa tttgatgaat cgagaggaaa cagcagagaa gatcacttgt tgattctccc
    12661 taaataaaaa cttcaaataa tcactgagat gagcgtaatc ttcaaagtct gtactgactt
    12721 ggtttgctgc taataatacg gtcatttttt tccctgctaa agagcgattg aaagattcag
    12781 caagtttcaa tgtttcactg atcgtaagtg agcctttgaa caaaccgttc aagaaatgat
    12841 tttttttcaa ttcgaaaaca agatcagaag aagtgtgcgt ttctttttgc tgcaaatcta
    12901 aagcagttac tgctttttct attgcttgtt tcaattcgct acttttaatc ggtttaagca
    12961 gataatcatc tgcctgtacc tggattgctc ctttcatata atcgaaatcg tcgaatccac
    13021 taataaatat gagacgagtc caaggaagta gtttccttgc ttcttttgaa aaagtcaacc
    13081 cgtccatgaa aggcatttgg atatcagtca gaatgatgtc gggctgtaag tcgataattg
    13141 aagacagtgc catctcacca tctccagctt cgccgacata agtgattgga taataagaac
    13201 caaggacagt tatttgttcc cgcaagttat tgcgaatgag gtattcgtct tcgatgataa
    13261 aaactttata catgtttgtt tcctccaatt gttttgggag atagagggat catcagtgtg
    13321 acagttgttc cttctcggta tttgctgtca atcgtgatag gtttttgatc atttccgtaa
    13381 taaagaagga gacgcttgtt tacgttgtaa agtccgtagc cgatactgaa atctgaatcg
    13441 atccctttgg ctaactcttt acgtatttct gccagttttt ctgcagggat gccaatcccg
    13501 ttgtctataa ctttgaattg gatcatctca tcaattttgc atgtggtaat acgaataaat
    13561 cctttgtgac ggacgtactt cgttccatgg tatacagcat tttcaatcaa tggctgcaag
    13621 atcattttta aaatagaaac aggaaaaagt gtttctggta tatcgatctt atagtcaagc
    13681 atatggccgt atcttatttt taagatgact agataatcat tgatatggct aatttctttt
    13741 tcaagaggaa tccagtcttg tcctttgctt aaagacgtac gaaaaaagcc ggaaagtgca
    13801 tgggtcatag tcagagcttc ttcaaagcag tcttgttcga tcaaagaaat aatggcatcc
    13861 aaactgttat aaataaaatg gggagtaatc tgagcttgca atacacgaac ttcactttgt
    13921 gctaaatggt attgtttcaa agcgttctcc cgtaaagact ggtttagatc ttccgccatt
    13981 ttattcatgc tagcggtaag tgtttgtaat tccagtgttt ccggcagggt taaacgaaag
    14041 ccaaggttcc ctgaagaaag ttgttctgac atctcgatca gctgattgat cggttcttgg
    14101 acctttctct ctaaaaagcg acgattcttt ttcgcataga aaaaaatggt actaagaact
    14161 ccaagttcca ctattgttaa tagaatgagc gagcggacaa gtgtcgtatt ttttttgctt
    14221 gctaagtcga tctccacacg aacaaaatct tgtaagatat ctgacagcag ttgagtgaca
    14281 gaatctacct gattgatgat ctcttcattg gtatccataa aatcttttgc caagatatta
    14341 ttgatcagct cgttttgata atcttccagt gtatcgatcg tacgtagcga tacatctaac
    14401 gtactaattt ctttaagtgt atttgtatta tttttgatct ctcgaatatc attcctaagt
    14461 tcttctagga tattttcctc attgaactca tctacggtta cttggccaga aatcaagtcc
    14521 cacatacttt ccaaaacttt ttgatcaacc ttcaaagcaa ttgcatctgc tttttggata
    14581 tttgcgatcg tctgttgata aatcaaaagc tgttgcgaat ataacagtat gctcagtagc
    14641 aaagggataa cagcaaatag aagcatggct ttgtttgctt gggcaaaaat ctgttttatg
    14701 cttaaatttt ttgtcatgat acttctccag tcaataattt ctggttggga tgttagctat
    14761 attctgctgg gaaaagacag tctcggaaat aaaaatatct ttaggtaatg tttttcctgt
    14821 ttgaatattt ccaaaataac gagtaacggt attggctaca taccagccga catcaggttt
    14881 acactcgact acacaattga cgattcctga gcgtagcttc tcgatcatat cttcttgtcc
    14941 atcaatagtt acaatgatca aatcttctcc aggtttgatt gatgtttctt ttatcgcagc
    15001 taaagcaccg tgagtcattt catcactatg gctgtataat acatcgattt cggataaatc
    15061 atttgtttct aaatatcgtt tcatctggtc ttttcctttc atccgaataa aatcaccgac
    15121 aattcggtga acaatagtca tcggaggttt atggtcgatg gttccatctt tttttagggt
    15181 tggtttacga ttgattgatt ccaaaaatcc ttcggagcgt agcttggtcg gtgtggagtt
    15241 ctctgaacca gcaagttcta ggattctgat ttcctgtttc tttgaatttg tgaagtgatt
    15301 gctgacaaat aaacccgccc gatttccttc tgctttgaaa ctggggccaa tgtgggtgac
    15361 gaaaagctcg ggatcattcg tttgaatatg tctgtcgaca agtatgacag ggatccctgc
    15421 tcgctttgct tcttttaata cagagtccca cccggattct tgcaaaggag taaaaatgat
    15481 catatccact ttataggcaa tgaacgtccg tatatcttgg atttgccgtt cctgattcat
    15541 gtagccattt cggtagagaa cttggtaatc ttctttttca agcgcagtga taattgattc
    15601 agtatgcttt ttccgccagc tactctccgt tcctgattgc gaaaaaccaa cgactagtgg
    15661 gtcaggtgct tcctttgtat taggtgtaca ccccatcatt tggtaagcga ttacaacaag
    15721 ggctaaaaaa aataaaacac gtttcacttt aaatcactcc atctggattt gttagtacta
    15781 tcttatcaga taaaagggaa aatcaaagtc aaaaggaatc aatcgaaaaa aatccaagaa
    15841 acttaaaaaa atccaagaca aaaaaagtac acctaaaaaa tttaaagtaa atcccataaa
    15901 aaaatcaatg ttaacgcttt ctttattagc tatactcggt gtatcaattg aaatggggga
    15961 taaaaaatga aaaacaaatt tagaaagatt gttgggacta tcgctatttt gaccgcaagc
    16021 attctattaa gtgcgtgtgg caattcagga tctgcagatg atagtacggg gtatgtagga
    16081 atcgctatgc cgaccaaatc agcagaaaga tggatcgctg atgggaacaa catggtctcg
    16141 gaattagaaa aactgggtta taaaacagat cttcaatatg gagaagataa agtagaaaat
    16201 caagtagcac aaatagaaaa tatgattaca aaaggggtag atacacttgt gattgcttct
    16261 atcgatggtt cggctttaac tgatgtgtta gctaaagcaa aagaagcaga tatcaaggtg
    16321 atcgcctatg atcgtttact gatgaattca gaaaatgtcg attattacgc gacttttgat
    16381 aattttggag taggtgtatc gcaagcagct tatattgaag agcacttagg attgaaagaa
    16441 ggaaaaggac cattcacgat tgaattattt ggtggctcgc ctgacgacaa caacgcacta
    16501 atcaattaca acggagtgat gtctgtttta caaccatata tggacaacgg tcagttggtc
    16561 gtgccatctg gacagacgag tttcagtcaa atcgctacgc ttcgctggga tggttctacg
    16621 gcgcaagcaa ggatggataa tctgttaagc gcaaattata ctgaccaaac attggatgct
    16681 gttttgtcgc catatgatcc aataagttta ggaatcattt cgtcattaaa aggcgtgggg
    16741 tatgggagtg aaagtaaacc tttacctgtg attactggac aagatgcgac agttgctggc
    16801 gttaaatcga tcattgccgg ggaacaaaca caaacgatct ttaaagacac acgtatctta
    16861 gcaaaaaata cgattgaaat gatcaaagca atcagtgatg gagaagaggt tccggtcaat
    16921 gatactgaaa cttatgataa tggggtgaaa actgtcccta cgtatttagc aaatacagta
    16981 tctgtcgata aagataatta tcaagcagag ctgatcgaca ccgattatta taaagaatcc
    17041 gatctgaaaa attaagagga accaaacaag gaactaaaga gatctaagtc agagagagga
    17101 taagaattat ggcggattgt attttaaaaa tggcagatat caccaaaaaa ttttctggaa
    17161 tccgagcgtt agattgcgtg aatctgatga ttgaacgtgg ggaaatccat gcattgtgcg
    17221 gagaaaacgg agcgggaaaa tcaacattga tgaatgtctt gagcggcttg tatccatatg
    17281 gctcctatga cggggaaatc ttttatgatg gaaagctgtg tcaatttaaa gatttgaaag
    17341 atagtgaaga aaaaggcatc gtcatcattc atcaagaatt agcactaagt ccttatcttt
    17401 caataaaaga gaatattttt ctcggaaatg aacaagcaga aagaggcgtg attgattggg
    17461 aactgacaga aaagaaaaca tatgaactgc tgaataaagt cggtttgtct atcgacccga
    17521 atcttctagt atcccagatt ggcgtagggc aacagcaact agttgaaata gctaaagcat
    17581 tatcaaaatc cgtccgtctg ttgattttgg atgagcctac cgctgcattg aatgaagaag
    17641 aaagcgcgaa tttgctgaga ttgatccgag agttgaaaga gcaaggtgta acctccatca
    17701 ttatttcaca taagctgaat gaaatcgtgg atatcgcaga tcgaatcacc attttacggg
    17761 atggacagac gattgagaca ctagaaaatg aaatgatcag cgaagagcat attattcgcg
    17821 gaatggtggg cagagattta gttgatcgtt atcctgaaag aaaaccggat attggagaag
    17881 tttattttaa agtagaagac tggacggtct atcaccctct tgatcacgag cgaatcataa
    17941 acgaccacat caacttgcat ttgaaaaaag gagagattgt aggaatagct ggactgatgg
    18001 gagcgggcag aactgaattc gctatgagtc tatttggaca ttcttatgga agcaagatat
    18061 ccggacgtat attaaaggaa ggggcagaga tatcagttaa gaccgttcct tcagccattg
    18121 caaacggact cgcttatgtc tcggaggacc gaaaatcttt aggactgaat ctgctgatgg
    18181 atattcggga aaacgtgacg ctgagcagtt tggataaaat aagtcaaaaa ggagtattag
    18241 ataaagaaaa agaagtaact gaggcagaaa atttccggaa aaagatgcgt ataaaaacaa
    18301 actccatcca tcaaattgtc agtagtctta gtggtgggaa tcaacaaaaa gtcgttttat
    18361 ccaagtggct gatgacacaa cccgatattc tttttttgga tgagccaacc cgtgggatag
    18421 atgttggggc aaaatatgag atctacacga ttatcgaaga aatggcagca aatggcaaag
    18481 ctgtctgtct tatctcttct gaacttcctg aaatattagg gatgtgtgat cgaatctata
    18541 cgatgaacga aggacgtatt acaggagaag tctcgcgaga agatgcagat caggaagtct
    18601 tgatgaaatt aatgacgaaa gaagaggtgg gttaagttgg aacaggcaaa aaatcttacg
    18661 gataaaaaag agaaaaaact aaatttgaaa agtagagcag ctgatctttt tggtcgctac
    18721 agcatggtga tcattttagc gggactactt attgcgtttc aattgatgac ggatggtatt
    18781 ttctggcgtc cattgaatat aactaacctt gttctgcaaa atagccatat attagtacta
    18841 gctgcaggga tgttattagt tgtgctctta ggtcatgtgg atttgtcggt agggtcagtg
    18901 atggcgtttg ttggtgcaat tgcgggcatg atgatggtca atcgccaaat gaacccgtgg
    18961 atgacagtta tcgcatgtct tgcagtaggc gggctgatcg gcgcatggca aggtttttgg
    19021 gtagcttatg cagggatacc ggcgtttatc gtgactttag caggattgct gatgtttcgt
    19081 ggactgacac aagttgttct aggaggccaa tctctagcac cttttccagc tgttttccaa
    19141 aaaatgtcta cgggttattt accagactta agcgaaggaa gcagccttca cttcttgaca
    19201 ttgattttag gaatgatcat cacgatcgtg ttagttgttg gtcaatggcg aacaagaaat
    19261 cgtcggaaaa atcatttatt tgaagtggaa tctccaacag ctttcttggt aaaggcggta
    19321 tttacagcag tcgttgtact aggaataact tatatctttg cctcttatca agggtatccg
    19381 attattttga tcattcttgg tttgatcgtt gcagcatatg ggtttttgac aaataaaaca
    19441 gtagcaggtc gacagattta tgcgacgggc ggaaatcgaa aagcggctga actatcaggg
    19501 atcaaaacaa agcgaatcac gttctgggta ttcgtcaaca tgggagtcat ggcagcttta
    19561 gctggattag ttttagctgc tcgattgaat gcggcaacac ctcaagcagg tacatcgatg
    19621 gaactggatg caatggcggc agtatatttt ggtggtgctt cgacttccgg cggcattgga
    19681 acaatcatgg gtgcgattgt cggtggcttg gtcatgggtg tattgaacaa tggcatgtcg
    19741 attttaggcg tcggggtaga ctggcagcag gcaatcaaag gattgatctt gcttttagcc
    19801 gttgtgctag acttgtataa taagaaaaaa cgaacaacgt aggagaacgc cggttattca
    19861 gaaaaaagac tgtgacaaga catttgtcac agtctttttt tcgctaaaag cgaacattca
    19921 ttgtttcgtt tgtatagact aaaaataata tatgtttttt taccattcgt ataaattctc
    19981 tttttgctct tgattttttt gcttaattga gttaaattac atagggcgct aaaaaaagcc
    20041 ttgattaaat taataatgtt cgtgttttgc tggatgtgaa catcaggagg ataatgtaat
    20101 gaaagtattt gattacgaag atattcaatt aattcctaat aaatgtatcg taaatagccg
    20161 ttctgaatgt gatacaacgg tcacattagg taaacataca ttcaaaatgc cagtagtgcc
    20221 tgccaacatg cagacgatta ttgatgaaac aatcgcagaa ttcttagcgg aaaatggtta
    20281 tttctatatc atgcatcggt ttgatgaagc agctcgaatt ccgtttatca aaaaaatgaa
    20341 aaagcgtgga ttgattacct cgatcagtgt tggcgtcaag aaagaggaat attcctttat
    20401 agagaagttg gcagaagaat cgctaaatcc tgattatatc actatcgaca tcgcgcatgg
    20461 acatgctaac tcagttattg atatgattca gcatattaaa aaatatttgc ctgaaacttt
    20521 tgtgattgcc ggtaatgttg gtacaccgga agccgtccgt gaattggaaa atgcgggcgc
    20581 agatgcaaca aaagtcggga ttggtccagg aaaagtatgt attacaaaaa tcaagacagg
    20641 ctttgggact ggaggctggc aactggcagc tcttagatgg tgtgcgaaag cagctcgcaa
    20701 gccgatcatc gctgacggtg ggatcagaac acatggcgat attgcaaaat ctgtccgttt
    20761 tggtgcgaca atggtcatga ttggttcatt gtttgccggt cacgaggaat ctcctggcga
    20821 aacaaaagtc gaaaatggaa tcgtgtataa agaatacttt ggttctgctt cagaattcca
    20881 aaaaggcgaa aaacgaaatg ttgaaggaaa gaaaatctgg atccaacaca aaggatcttt
    20941 gaaagataca ctagtggaaa tgcaacaaga ccttcaatca tctatttctt atgctggagg
    21001 aagagatctg gaagccattc gaaaagttga ctatgtcatc gtgaaaaatt cgatttttaa
    21061 tggagacaca atctaaaaaa taaataaaat attaggatgt tgtgaaaaag ttgtcctgca
    21121 ttaagtaata agaacagcca aacaaaaata gttctttata tgttttgttt ggctggaatt
    21181 attatcgcag atacaagctt ttgaacatca ttattcagaa taagggactg tgataagatt
    21241 tttgtcacag tcccttatgt tttgcaaaaa aagtttttct tttttctcgt aaattcggta
    21301 aaatcaaaag aaaaaataga caatagaaaa gagaagctta tgtgggaaaa agtagagaaa
    21361 aaacaaggac cgatttatcg tcaaattatg gaacagatta tgagagaaat cgaaaacgga
    21421 aggatcaatc ctggaagtca acttgaatca gaacgaaaac tagggcaact ttttggtgtg
    21481 aatcgttcca ccgttgtcca tgcattggaa gagcttaggg agcttggggt actaacgagc
    21541 aaacgtggca gcggatggtt tgttaatcag actgaatggg ggaaattcgc tgttccaaga
    21601 atcgattggc ggcaaatgat ctctcctcgt tatgagcaga cggactggta tgagcaaaaa
    21661 ataagagaag caaagcagat ccaaaaaaca tcgtttcttg atttatacgc tagcgagatg
    21721 ccagatgagc ttttgccgaa ttttgagttt ccttcttatt ctcttgaaga aatcttagcg
    21781 gaagaaaaag aaatggatct tttaggctat actccgttac gtaacaaaat caaacggtat
    21841 ttggaaagta aatttgaatt ggcattttcc cccaaccagc ttcttgtgac tggtggcggt
    21901 cagcaagcaa tctttttgat tttacaaacg attctttcag ctggagaggg gatagctgta
    21961 gagtcacctt ctttttttta ccgattacct cttttcaaag cttcaggaac gcgtttattt
    22021 gggataccga tggatgaaga agggatcgac ttagaaaaac tgaaagaatc gatccataaa
    22081 aataaattga aagcagtgct tatcaacccg aattttcaaa accctacagg aaaagtgatg
    22141 agtcaaaaac gacgagaaaa gctagtagaa ttgtgtcgac agtatcggct tccaattatt
    22201 gaagacgatg tatttacgga tctttcattt ctaaaccaag aagaagtcct tcctatacga
    22261 acgattgatc cggataatgt gttatacgtc ggttcgttat ctagggtttt aggaaaaaca
    22321 acgaaaatcg gttgggtgat aggacctgag acttttatct cgcaactagc tcaagcgcaa
    22381 gaaatgatgg agttttcgat gaatatcctt acacaaatca cagtggctaa tgtatttaat
    22441 gaagcaattg atttgaaaat gcaagatgta agaaaaaagt tgaaagaaaa aagccgactg
    22501 ataagcgctt ggggagattc acaatcgttt ttcaagcttc attcaatttt aggtggttac
    22561 tatgcctgga ttacttggga tgggaaaaaa ctagaccccg aactagcaga aaaagtagta
    22621 gatatgggtc ttggaattgc accaagtcta ctttttgggc aagatataaa tggaatcaga
    22681 atcaattaca gtaggctgga tgaaaaccaa ataccgttgt tttattcaaa gatgaaaatt
    22741 ctagaaaagt ggttaacaga ataatttcac aaagtaaata gattaaagaa tataacagct
    22801 tggatttaaa tttcaagctg tttctttttt ttcggaaaaa gaatacgata taaagaaact
    22861 aagaaaaaac cattatttgt acgtccgttc acgatgtgta aataaaataa atgtttgcaa
    22921 caaaactgta taacaccaac gtttttaaat agaaacagat ggttgtaagt ttgattagaa
    22981 aatagaaaaa atgcagatga aatataaaac gctttcctta ttgtgatttt attcactaat
    23041 gctgttaata tgcggattgg cataatttcc tttcatcgtg aaaatttgtg cttttagctt
    23101 gtatttctca taaatccatg ataatttatt aatgaagacg gttaaatagt gatacagttt
    23161 tattcactgt ttcaatcgca gaatgaagag attttcttat agatgagaga ggaatgtgtg
    23221 acaaatggca aacaaaaaag caccgatcga cttccaagca ctgctagatg aagtcaatgc
    23281 agatttccca gtatatcaag cacttgatca agatggaaac gtagttaaca aagatttagt
    23341 acctgattta tccgatgatg aactcgttga attaatgact catatggtat ggtctcgtgt
    23401 attagaccaa cgttcaacag cgctgaaccg ccaaggacgc ctaggctttt ttgcaccaac
    23461 tgctggacaa gaggcaagtc agctggctag tgcttacgca tttgataaag aagatgttct
    23521 tcttccaggt tatcgggatg tacctcaact agtaaaacat ggtttacctt tatctcaagc
    23581 ttttttatgg tctcgaggac atgctgcagg taattcttat ccggaagaat tgaaagctct
    23641 accaccacaa attatcatcg gtgcacaata tgtacaagca gccggtgttg cattaggttt
    23701 gaagaaacga aacaagaaaa atgtcgtctt cacttatact ggtgatggcg gttcatcaca
    23761 aggtgatttt tacgaagcaa tcaatttcgc tggcgcttac catgcaaatg ccgtatttta
    23821 tatccaaaac aacgggtatg cgatctctac acctcgtgaa aaacaaactg cagcaaaaac
    23881 acttgcacaa aaagctgtcg cagctgggat tcctggtatt caagtcgatg gaatggatcc
    23941 gcttgcagta tatactgtat ctaaaatggc acgggaatgg gcgatcagtg gaaatggacc
    24001 agtcctaatc gaaacactga cttatcgtta tggaccacat acactatctg gtgacgatcc
    24061 aacccgctac cgtacaaaag acacagatga cgattggcag aaaaaagatc cattgatccg
    24121 cttccgtaaa tatttgacgg aaaaaggact ttggtctgaa gaaaaagaag aacaagtcat
    24181 cgaagcaact aaagaagaaa tcaaagcagc tattgctgaa gccgacaaag taccaaaaca
    24241 aaaggtttca gatttcttga aaaacatgtt cgaagtatct ccacaatcaa tcaaagaaca
    24301 aattgcaatt tatgaagcga aggagtcgaa ataatcatgg cacaaaaaac aatgatccaa
    24361 gcaatcacag atgccttagc acttgaacta gaaaatgacg agaatgtcgt tgtttttggt
    24421 gaagatgttg ggaaaaacgg aggagttttc cgggcaactg aaggattgca ggaaaagttc
    24481 ggagaagacc gagtattcga tactccttta gctgaatctg gaatcgcagg attgagtttc
    24541 ggtcttgcgt tagaaggatt tcgtccagtt cctgaaatcc aattctttgg ttttattttc
    24601 gaagcaatgg acgaagtagt agctcaaatg gctcgtacaa gatatcgaat gagcggcact
    24661 agaaatctac ccatcacaat tcgttcgcca tttggtggcg gtgttcatac accagaattg
    24721 cattcggata atttagaagg tttgatcgct cagtctcctg gtattcgagt agtgattcct
    24781 tccaaccctt atgatgcaaa aggactattg atttcttcta ttagaagtaa cgatccggtt
    24841 gttttcctag aacacatgaa actatatcgc tcattccgag aagaagtgcc ggatgaagct
    24901 tacgaagttc ctttggataa agcagcagtt actcgtgagg gaacagatgt atctattatc
    24961 acatacggag caatggtgcg tgaagcaatc aaagcagcag acaatcttgc aaaagaaaat
    25021 atatctgttg aaatcattga tcttcgtaca gttgcgccat tagatgtaga aacgatcatt
    25081 caatccgtgg aaaaaactgg tcgcgtagtt gttgtccaag aagcacaacg acaagcgggc
    25141 gttgccgcgc aagtagtttc tgagatctca gaacgcgcta tcctttcatt agaagcccca
    25201 atcggacgag tttctgcacc tgatacagtc ttcccatttg gacaagcaga aaatgtctgg
    25261 ttgccaaatg caaaagatat cgaagacaaa gtaaaagaaa tcgcggaatt ttaagaaaag
    25321 aagggacgaa acaaagtcat ggcttatcaa tttaaattgc cggatatcgg tgaaggaatc
    25381 gcagaaggtg aaatcgtcaa atggttcgtc aaaccaggag atacgatcaa tgaagatgat
    25441 acattactag aagttcaaaa tgacaaatca gtagaagaaa tcccgtcacc tgttacagga
    25501 acggttaaaa atgtgattgt tccagaaggt accgtagcaa atgttgggga tgtattagta
    25561 gaaatcgatg caccaggaca cgaagacaat gaaggggaca gtggtgtagc cgcagaatcc
    25621 caaactccag ctaaaccggc tgctgaaccc actgttgata cagaatcagc tggatcgtca
    25681 agcgaaggag tcttccaatt taagttaccg gatatcggtg aaggaatcgc agaaggtgaa
    25741 atcgtcaaat ggttcgtcaa accaggagat acgatcaatg aagatgatac attactagaa
    25801 gttcaaaatg acaaatcagt agaagaaatt ccgtcacctg tcacagggac agttaaaaac
    25861 gtgattgttc cagaaggcac tgtagcaaat gttggggatg tattagtaga aatcgatgct
    25921 cctggacaca atagtgcacc gtcaactagt gcgccatcag ctgaagcacc aaaagaaaaa
    25981 gtggaaacat caggttcagc aagtgtcgtt gaagctgccg atccaaataa acgtgtattg
    26041 gctatgccat ctgttcgtca atttgcccgt gaaaaagatg tagatatctc acaagtaact
    26101 gcaactggaa aaggtggcag agtaacaaaa gaggacatcg aaaacttctt ggcaggtggt
    26161 ccaagcagtg caccagcgaa atcagaagcg ccagaagcgg cagcaccgaa agaagcagct
    26221 cctgcagcag aaagcaaacc agctgcacca gcaaaaccat tcaagtccaa cttaggagac
    26281 ttggaagaac gtgtagcaat gacgccaaca cgtaaagcta tcgctaaagc aatggtcaac
    26341 agtaagcata cagctccaca cgtgacattg catgatgaag tagaagtgtc taaactatgg
    26401 gataatcgga aacgatttaa agaagttgct gctgcaaatg gtacgaaact tactttcttg
    26461 ccatatgtcg tgaaagcttt aacagcgact gttaagaaat acccagtatt gaatgcatct
    26521 atcgacgatg caaaccaaga aatcgtttat aaacattact acaatatcgg tattgcaact
    26581 gatacggatc acggactata tgtaccaaat gtgaaagatg cagaccgcaa aggaatgttt
    26641 gctattgcag acgaaatcaa tgagaaagca aaattagctc atgacggcaa actatctgca
    26701 gaagatatgc gcaatggaac aattacgatc agcaatatcg gatctgtagg tggcggttgg
    26761 tttacaccag tcatcaacta tccagaagta gctattttag gtgtaggaac gattgcacaa
    26821 caaccaatcg taaatgctga aggcgaaatc gttgtcggtc gtgtgatgaa actgtcatta
    26881 agtttcgatc accgtatcgt agatggagca actgcgcaac aagcaatgaa taatatcaaa
    26941 cgtcttttag ctgatccaga gctattaatg atggaaggat gataaacaaa tggtagttgg
    27001 agattttgct gtagaattag atacagttgt aattggtgcg ggacctggtg gctatgtagc
    27061 tgccatccgc gctgctgaaa tgggacaaaa agtagcaatc attgaaagag agtatattgg
    27121 cggtgtttgt ttgaatgtcg gctgtattcc ttcaaaagca ctgatagctg caggacacca
    27181 ttatcaagag tcactagatt ctgaaatgtt tggagtaact agtgaaaacg tcaaactaga
    27241 ttttgcaaaa acacaagaat ggaaagaaaa caaagttgtc catacgttga cttctggtgt
    27301 tggtttccta ttaaaaaaac ataaggttga aacaatcgaa ggagaagcgt tcttcgtaga
    27361 tgatcacaca ctacgcgtca ttcatccaga ttcagctcaa acttattcgt tcaataacgc
    27421 aatcatcgca acaggttccc gtccaatcga gattccagga tttaaattcg gaggacgtgt
    27481 gttagattct actggcggat tagcattgaa agaagtacct aagaaatttg tgatcatcgg
    27541 tggcggtgtg attggtgccg aattgggcgg agcgtacgca aacttaggag cagaagtaac
    27601 gattcttgaa ggctcaccac aaattttacc aacttacgaa aaagatctag taaaattagt
    27661 agaagatgat ttcaagaaaa aaggcgtaac agtcgtaacg aatgcaatgg cgaaagaagc
    27721 agtcgataac ggtgacagcg tgacagtgaa atatgcagtg gacgggaaag aagaatccgt
    27781 tactgctgat tatgtgatgg tcactgtagg tcgtcgtccg aatacagatg atatgggctt
    27841 agaacaagcg ggtgtcgaag taggagaacg aggattgatc acggtggata aacaaggacg
    27901 tacgaacgta cctaacatct atgccatcgg tgatatcgtt ccaggggcgg cattagcaca
    27961 caaagccagt tatgaagcaa aaatcgctgc tgaagcaatc tctggcaaaa aagtagctgt
    28021 agattataaa gcaatgccgg cagtagcctt cactgatcct gaattggctt ctgtaggtat
    28081 gacgatcaaa gaagcaaaag atgctggtat cgaagcaaaa gcatataaat tcccattttc
    28141 agggaacggt cgggcattat cgcttggtaa aacagaagga ttcatccgtc tagttacgac
    28201 gatcgaagat aacgtgctta ttggcgcgca aatcggtgga gtcggcgcaa gtgatatggt
    28261 ctctgaatta gctttagcaa tcgaatcagg aatgaatgct gaagatatcg ccttgacgat
    28321 tcatcctcat ccatctcttg gcgaaatcgt catggatgct tcggaattag cattaggttt
    28381 accaatccat atttgatttg aataatacag gcgaaacgca actgaaaaat cagttgcgtt
    28441 ttgtttttta gaattatcaa taatattatt atgtaaactt aaaaatatga caacttcttg
    28501 gtttattacc acttcctatg ctacactgaa gaggaatatg aaataggaga gttgaagatg
    28561 caagaatatc agtatccact tgatttagat tggacaacag acgaaatggt cattgtaatg
    28621 aagatgtggg aagatctgga acgtgcgaat gaaaaaggga taaacaatga agcttttttg
    28681 aagacttatc agcagtttaa aactgttgtc aaatccattg gtgaggaaaa acgattggga
    28741 cgggaatttg aaaaagcttc cggttattct ttatatcgta cagtgcagca agcaaaaaaa
    28801 aatccaaaca aaactttgag aatgaacggg tgagataaat ggaacagatg attgatcata
    28861 tcaagtcctg gcttgaagaa gctggtgaga aaatcaaatc ttctcaacaa acattaacaa
    28921 tctcgcaaaa atcaaatcga acggatctag taacaaatgt cgatcgaaac atccagaatt
    28981 ttctaatcga aaaaatccat tcctttgatc cagaggcgaa aatattggca gaagaagaag
    29041 ggtgtaatca attatcggat ctcgatggaa gagtctttat catcgatcct attgatggca
    29101 ccttgaattt tgtgattgaa ggggaaaatt tttgtattat gctcgctgtt tatgaatccg
    29161 gcgttggaaa gcttggtttc atttatgacg tgatgagaaa tgaacttttt tggggtggaa
    29221 aaggaatagg cgtgtataaa aatgatgagc cattgcctag accagcaaat aaatccttat
    29281 cggaaggctt acttggtgtg aatagttatc ttttcggtca taataaatat ggaatccgag
    29341 aaattgcgga aagcagcatg gggacacgta tgtacggatg cgctggtctt gaactagtgg
    29401 caatcttgaa aggaaaccac attgcttata tttcaaacct tagcccttgg gattatgcag
    29461 cgggaaatgt cttattggaa gagtttggta tggtaacaag cggtcttttt ggtactcctt
    29521 tgacatttga aaaaagggaa tactatttag cagggacttc ttgtgcatat gcagaaatcg
    29581 cagacctttt gaaaaaagaa ggctgaaaaa aaggtagaaa tcattcacaa aaatgataaa
    29641 aacgagcaaa agattaattt tctcgaaaaa atgaaaacat tttactatat ttcatgaaaa
    29701 aagtctgcta taattagcag tcgagtaaaa tttgccctgt cataaagaac caataaaaaa
    29761 ggagttagtt acattgaatt atagaaacga tatccgtaac gtcgcaatta ttgcccacgt
    29821 tgaccacgga aaaacaacat tagttgatga actattaaaa caatcagata cattagacgc
    29881 acatacgcaa ttgcaagaac gtgcgatgga ctcaaatgcg ctagaaaaag aacgtggtat
    29941 cacgatttta gccaaaaaca cagctgtaga ctataaaggt attcgcgtaa acatcatgga
    30001 tactccagga cacgcggact tcggtggcga agtagaacgg atcatgaaaa tggttgacgg
    30061 cgttgtgttg gtcgtagatg cgtatgaagg aacaatgcct caaacacgtt ttgtattgaa
    30121 aaaagcactg gaacaacata tcacaccaat cgttgtcgta aacaagattg acaaaccttc
    30181 tgctcgtcct gagcatgtcg tagatgaagt tcttgaacta ttcatcgaat taggagcaga
    30241 tgatgatcag ttagactttc ctgtgattta tgcatctgca ctaaatggta cgtcaagctt
    30301 gtcagatgat ccagcagatc aagaaccaac catggcacct atttttgata caattattga
    30361 aaaaatccct gctcctgtcg ataacagcga cgaaccatta caattccaag tttcattact
    30421 tgactacaac gattatgtag gtcgtatcgg aatcggacgt gtatttagag gaacaatcaa
    30481 agtaggagac caagtagcat taattaaatt agacggaaca gttaagaaat tccgtgtaac
    30541 gaaattattt ggtttctttg gactaaaacg tctagagatt caagaagcaa aagctggaga
    30601 cttgattgct gtctcaggta tggaagatat ctttgtcggt gaaacagtta ctcctgtgga
    30661 tcatcaagat gcgttaccta tccttcacat tgacgaacca acattgcaaa tgactttctt
    30721 agttaataat tcaccatttg ccggtcgaga aggtaaattc gtaaccgctc gtaagattga
    30781 agaacgtttg atggcagaac ttcaaacaga tgtatcctta cgagttgaac caacaaactc
    30841 tccagatgca tggacagttt ctggtcgtgg agaattgcat ttgtctatct tgattgaaaa
    30901 catgcgccgt gagggatacg aattgcaagt atctcgtcca gaagttattg aaaaagaaat
    30961 cgacggtgta aaatgcgaac cgtttgaacg cgtgcaaatc gatacaccag aagaatatat
    31021 gggtagtgtt atcgagtcct tgagcttacg taaaggtgaa atgcaagata tggtccatac
    31081 aggaaacgga caaatccgtt tgacattctt aacacctgcc cgcggtttga ttggttattc
    31141 aactgaattc ctttcaatga cacgtggtta cggaatcatg aaccatactt ttgaccaata
    31201 tctgccaatg cttcctggtc aaattggcgg acgtcaccaa ggggcgcttg tttcgatcga
    31261 tacaggaaaa gcaacgactt attctatcat gagtatcgaa gaacgcggta cagtcttcgt
    31321 agaaccaggt acagaagtat atgaaggaat gattatcggg gaaaacagcc gtgataatga
    31381 tttgactgtc aacatcacga aagcaaaaca aatgacaaac gtacggtctg ctactaaaga
    31441 ccaaacatct gtcatcaaaa aaccaaaaca attgacgttg gaagaatctt tggaattctt
    31501 gaatgatgat gaatattgcg aagtaacacc agaaagcatt cgcttgagaa aacaaatttt
    31561 agagaaaaat gctcgtgaaa aagcatctaa aaagaaaaaa taatctgtaa aaggatgaag
    31621 agagaaacca gcttgttata aaaaatagct ggtttctttt tttgtccaac tcttttctgt
    31681 ggagaagtca tctttgtcaa tggacataaa ttcagaaaat acgccgaaag acgtttaaaa
    31741 gacttttaaa aaacttattt ctagagagtt tttgtttcat aataagataa tgtttcacga
    31801 aaggagatca acttgtcctt cgaagaaaat cgaagtaaaa tggcaagaga aaggagcggg
    31861 aagatgaacg acttattaaa acaatacttt ggctatgatg agtttagacc tggtcaaaaa
    31921 gaaattatcc agaaagtcat tgatcaggaa aatgtattag gaatcatgcc taccggaagt
    31981 gggaaatcaa tctgttacca gcttccagct ttactattag atgggcttac tgtggttgtg
    32041 tcccctttaa tctctttgat gaaggatcag gtggacgcgg cgaatcaatt aggtataccc
    32101 gccactttta tcaatagctc gttagatggt tacgaaacag ctagacgctt ccaagaaatc
    32161 gatagacagc agtaccgttt actttatatc gctcctgaac gttttatcat gccggatttt
    32221 atccaagcaa tgaagcgatg gaacgtccgt atgatcgcta ttgacgaggc acattgtatc
    32281 tctcagtggg ggcatgattt ccgcccaagt tatttacaga tggcgaacca gctagatcaa
    32341 ctgccaaatc gtcctgtaat tgttgctttg acagctacag cgactgtgca agtagctgct
    32401 gacataaaaa gacttttaaa aattccagaa aacaaccata ttcagactgg ttttgaacga
    32461 gaaaatctac gatttcaagt aattaaagat caaaaaaaag aacaatatct aatcgaatat
    32521 ttaaagatca acaaaaatca atctggtatc atttatgcag caacgagaaa agaagtagac
    32581 cgtctctatc atttgcttaa aaaattcgat ttttccgtgg gtagatatca tggtggtcta
    32641 aatgagaatg agcgaacaga aatgcaagaa gcctttttat acgatcgctt gcagctgatt
    32701 gtagcgacaa atgcatttgg tatgggtata aataaaagca atgtccgttt tgttatccat
    32761 tatcaaattc ctggctctct agaagcatac tatcaggaag ctggtcgggc gggaagagat
    32821 ggtctttcca gtgaagccat cctgcttttc tctcctcaag atatccaagt ccaaaagttt
    32881 tttatccagc agtcccaaag agaagaaggg caaaagcaaa aagaatatga aaaattaaaa
    32941 gcgatgacag aatacgtgta tattgagtcc tgtcttcagc aatacatcct aaactatttt
    33001 ggcgagacaa gcagtccgtg caaccgttgt ggtaactgtt tagatgacag agaaatagta
    33061 gaagtaacga cagaagcaca gatggttctt tcttgcttaa aacgtatggg tgagaactat
    33121 ggtaaacaaa tgctgatgaa agtcttagca ggatcgaaag aacaaaagct gcaagcttta
    33181 ggttttggac atttatctac ctatggactg atgaaaaacc aatcgcaaaa agaaacgatg
    33241 cagttaattg aatatttgat atcgaatggc tatcttctga ccatcaatgg tgagtatcct
    33301 gttttgaaag taacagaaag agggatacaa gtattgaaag gacaagaatc tgtttatcga
    33361 aaggaaccca agaaagtcca gcagctatcg gatgaagaaa ccgatacact atttgaagtg
    33421 ctgcgggaac tgcgaacgga tctagcatca gaagcaggcg ttcccccgta tgtcgtattt
    33481 tcagattcca cattgaaaga aatgagccga atacgtccat caagcagatt ggagatgctt
    33541 cagataaaag gagttggcca aagcaagctt gataaatatg gcgaagcctt tttatctcgg
    33601 ataaaaaatg cggactctaa cgttctggat aagtagaaag aacgaatacg taaaaatgaa
    33661 taaaaacaac tggttaaatc tgtttttttt gctccttatg aaaatcaaat atgttataat
    33721 taaggaatta gaatgggagg taagcacaga tggattcttt gtcgaaaaaa cttgcgatcc
    33781 aagtattaaa tgatgaagca gaacggatca aacgattaat ccgaaatcaa aacaacagtc
    33841 tctgtatctc acaatgcaaa gccttcgaag aagtggtaga tacacagatg tacggctttt
    33901 cccggcaagt cggttttgct gtccgtatag gagtaatcgg aaagaacgag ggccagctga
    33961 tgttaagtga gctagaacga gaattgaatc agttatatag tgaggtttac caagaaattt
    34021 atgataaaaa agaagttggc aaggaggaat agacttgcct aagaaagtga aaaaaaggca
    34081 tctattagac tatagcatct tgattcccta tttaatttta tgtgtattgg gattgatcat
    34141 ggtatatagt tctacgtcgt atctattatt agaaaacgga caaaatccgt ccgcatcagt
    34201 cattaaccag agtatttttt gggtgcttag cttgattgct atcgcactac tttataaaat
    34261 gaaaacagat gttttgaaaa accagcgttt gatcatggca gccatcgcag ttttgacgat
    34321 tttgctgctg attgttgtgt tttttggtaa ggagatcaat ggggcgaaag gttggctgca
    34381 aattgcagga ttctcgatcc aacctgccga atacctaaaa atcatttcta tctggtacct
    34441 gtctctgact ttatctaaaa gacaaaacag tgtacaaaaa gattttcttg ggactgtgaa
    34501 acgtccactt gcaatggtta tcggacttac tgcattagtc gctattcttc ccgattttgg
    34561 taatgcggca gttatctttt tgattatttt ggttctgctt ttagccagcg gagtcaatta
    34621 tgtgtatact ttgattgtag gagtaggagg cttttgtcta agtactttta cgatctggct
    34681 gatcaatatc acaaacggaa agatcttacc cggacgttta cagtatatct ataatcgatt
    34741 tgctatttac caaaatcctt tctcagacga attaaataaa gggcatcaac tagtcaacgg
    34801 ctactatgcc atgttcaacg gaggactatt tggcagggga ttaggtaaca gtatccagaa
    34861 aaaaggattt ttacaagaag cacaaaccga ttttatttat gcaatcgtag tggaagaact
    34921 gggagtgatt atggggattt tgatattagc gttgctattc tttatgattg tgcgcattgt
    34981 ccttgtaggt atccgttcaa aagatccgtt caattcgctt ttgtgcattg ggatcggcgc
    35041 aatgtttttg atccaagtat ttgtcaatct tggaggaatt actggcgtta ttcctttgac
    35101 agggatcacg ttcccatttt taagtcaagg aggctccagt ttgctgatgc tttcgatttg
    35161 tgtcggcttt gtcctcaaca tcagtgcgga tgaaaaacgc aagagcttag gtttataata
    35221 aaaaaagatg taggagtggt tcgatgaaaa aagttttagt tgctaacagg ggtgaaatcg
    35281 ctgtacgtgt atttcgtgct tgtacagaat taggaattaa aactgttgga atctatgcag
    35341 aagaagatga atattccgta caccgtttca aagcggatga agcatattta gtaggacaag
    35401 gaaaaaaacc gattgatgcg tatctagata ttgaagggat catttctatc gcgaaagaat
    35461 gcggagctga cgctattcat ccgggttacg gtcttttgtc ggaaaacttg aattttgcgc
    35521 aacggtgcga agaagaaggc atcatttttg ttggacccaa actgcatcat ttagatattt
    35581 ttggagataa aatcaaagca aaagctgcag ctatcgaagc ggggattgct tcgattccag
    35641 gcacagatgg accaatcgct tctatagacg atgccttgga gtttgcgaaa caatacggtt
    35701 atccgattat gatcaaggca gctctaggcg gcggcggacg cggcatgcgt gtagctcacg
    35761 atgaaaagag tgcaagagag ggctacgaac gtgcgaaaag cgaagcaaaa gcagcctttg
    35821 gtagtgatga agtgtatgtt gaaaaataca tcgctaatcc taagcatatc gaagtacaga
    35881 ttctcgggga tacgcatgga aacgtcatcc atctttttga aagagattgt tctgttcagc
    35941 gccgtcacca aaaagtagta gaagtagcgc cttgtgtctc tatgaatgaa cagcaacgtc
    36001 aaaaaatctg ccaggcagct gtccaattga tgaaatatgt cgggtatgtc aacgcaggta
    36061 cagtagaatt tcttgttgaa ggagatgatt tttactttat cgaagtcaac cctcgcgtcc
    36121 aagtggaaca tacgatcaca gaaatgatta cagatattga tatcgtaaca acgcaactgt
    36181 tgattgctca aggtttggat ctgcataaag aaatcggatt gcctcagcaa gaaggaatta
    36241 aattaaatgg ttcagccatt caatgccgaa tcactacaga agacccgttg aataactttt
    36301 taccagacac agggaaaatc gatacgtatc gttcacctgg tggttttggc gtacgtctgg
    36361 atgtcggaaa tgcttacgcg ggctatgtag tgacaccgta ttttgattca ttgctagtaa
    36421 aagtatgtac gcatggtgct acctttgaaa cagccattca gaaaatggaa cgttgcttga
    36481 aagaatttag aatccgtgga gtgaaaacaa acattccatt tatgctcaat gtcatcacac
    36541 atcctgaatt ccaatcagga aatgcaaaaa caaccttcat tgatagtaca gcaacattat
    36601 ttgaatttcc tcgactacga gaccgcggaa acaaaacgat gaaatatatc ggtgaaatta
    36661 ctgtgaatgg ttttccaggt atcgaaagtg gcgaaaaacc attttatgaa gaaccacgta
    36721 tgcctaaaga tctaatcaca agaactgatt acgtaacggc taagaatgta ttagatgcga
    36781 agggtgccga tgctttggtc gaatggatca aaggacagga aaatttgttg ttgacggata
    36841 cgacatttag agacgcccat caaagtctgc tagcaactcg tgttcggaca aaggatttca
    36901 agcagattgc acgtttgact ggagaaggtc ttcctgaact cttttcaagt gagatgtggg
    36961 ggggagcaac atttgatgtg gcttaccgct ttttgaatga agatccttgg caacggttga
    37021 gaaaaattcg ttctttaatg cctaatacgt tgctgcaaat gcttttcaga ggttcaaatg
    37081 ccgttgggta ttctaattat cctgacaatg tattggtgga atttgtcaaa gaagcagcag
    37141 cgcaaggaat cgatgtattt cgaatctttg atagtttgaa ctggactcca caaatggaaa
    37201 aaagtatcca agctgtacgg gatactggta aaatcgcaga ggcagctatt tgctatactg
    37261 gcgatatcaa cgatccaagt cgggcaaaat acaatgttca gtattataaa gacatggcaa
    37321 aggaactaga acaactgggt gcgcatatca ttgcaatcaa agatatggct gggctgctta
    37381 aaccgcaggc agcttatcgt ttgattagtg aattgaaaga aacgacagat ctgccaatcc
    37441 atctgcatac tcatgatacc agcgggaatg gaatcatcac ttattctgct gcttcaaaag
    37501 caggagtaga catcgttgac gtggcaatga gtgcaatgag tgggaatact agtcagccta
    37561 gcatgagcag cttgtattat gcactggtca atggtccacg gctgccagaa atcacgattg
    37621 aaaatgcaca gaaattaaac cattattggg aagatgtgcg catgtattac aaaccgtttg
    37681 aaaacggctt gaatgctcct gaaacagaag tatatatgca tgaaatgcca ggaggacagt
    37741 attcgaatct acagcaacag gcaaaagctg tcggattagg tcatcgctgg gatgaaatca
    37801 aacaaatgta tcatactgtc aacttgatgt ttggcgatat cgttaaagta actccatcat
    37861 ctaaagtagt tggagatatg gcactattta tggtccaaaa tgatcttaca gaagaagata
    37921 tttatgaaaa aggcgaaaca ctaagtttcc ctgaatctgt tgtgactttc ttccaaggag
    37981 aactagggca acccgttggc ggatttccta aaaaattgca aaagattatt cttaaaggca
    38041 gacctgcatt gaatgaacgt ccaggtcttt tggctgaatc agtcgacttc aacgaagtaa
    38101 aaaaagaatt ggcagaaaaa attggctatg aaccaaaaca agacgaagta ttaagctatt
    38161 tgatgtatcc gcaagtcttt ttggactatc aaaaagcata taatcaattt ggcgatgtta
    38221 cattactgga cacaccaaca tttttccaag gtatacgttt gggtgaaaca atcaatgtac
    38281 aaatcgaacg cggaaaaacg cttatcattc gtttagatga aatcggagaa cccgatatcg
    38341 aagggaatcg cgtactgttc ttcaatttga atggacaaag acgtgaaatc actatcaaag
    38401 ataattcgat catcagtacg gtccaaaccc gattgaaagc tgaaccgaca aaccgagaac
    38461 aaattggagc aacgatgtct ggttctgttt tggatgtatt agtgaaaaaa ggcgataacg
    38521 ttaaaaaagg tgatacgttg atgattactg aagcgatgaa aatggaaacg gccatcgaag
    38581 cacgttttga cggagaagtc gcacatgtat atgtgtcttc aggagacact atttcgtctg
    38641 gtgacctttt gattgaagtg actgagaaat aataagcgaa aggtggggaa acatgaaaag
    38701 actgggtttg ttttggctga ttctgctatt agttattgtg gcattctaca tggaaccagt
    38761 cttgtttccg ccaaaacttg aagtgaagga aaactctgat caacaaatat caaaccaaat
    38821 gacaacgtgg aaataccagg agcttaaagc aactggtttt tctacttata tcggaaaacc
    38881 gatcgatcag ctggaaaagg attttggcca agcctatgat cgattaacta gcggatttgg
    38941 ttttgaaaca agatattatg aaaataaagc tagtcatctc acatttgaag cgaacatcca
    39001 agacagcaga gtcacaactg ttaaagtact gcaaacaggg cctgctgata tcgcgccgtt
    39061 ttatttaggg atgacgatgc aagatcttac gaaaatcact actatctata ctaatttcac
    39121 ttttgagtac catgatacag aagttggaat cgaactaatg gaagaggata tgaattatcg
    39181 tccgctaatt gcttttgata acgacacttt tgctatttta ttttttgatc aaacaacaaa
    39241 cggcctattt tcagttgtgt atttagataa agatagtctt ttgaaactat tgccatatca
    39301 agtgtttggt gaaggccttc cacattatca agcagataaa tcggctgatt gggaatcaat
    39361 caatcgcgtt aaagaacgaa aaagtacgac tttattaaat atgcttagaa gagaagacga
    39421 acttcccata tacaatcaaa cacttgcctt tcaaaaccac agtcagctgc tactacatga
    39481 ttttttgaca aggcctgagg aaatattgtc agaggaacgt atggctgatt ggcaaaaatc
    39541 gctaggcagt gctcaaacga cagtgaaatt ctcactgacg aatgatgaat tggagaagtt
    39601 aatcagtaaa caaaaattag aaaatactag tgggctattt attcatccgg cgattgacgc
    39661 tactttttcg tttttatatt ggtacagcga tccttacaac catgaccgtt tcatggaccc
    39721 gggaacggat tctgtaggca ttgctttttc taaagaaaat atgttagtct tattgcaaga
    39781 agaaaacgga gaaagtagtg atagcagtga tcgttaacga agagctgttt caactggagg
    39841 atcaatgcct ccaagtcgct gaaatgataa aaaagagtaa aacgatggct tcttatcttg
    39901 aacataaaca gtcaatggaa gaatctgcaa aagtttgcca gttgaaagaa gcgttcttgt
    39961 ctaagaaaga agcatttgaa aatgtagcgg catatggtga gtatgcacct gattatcgtg
    40021 aaaaaaaacg agctgttcga aaagcaaaaa gacagcttga tatggatgag catgttgcag
    40081 ctttccgata tagtgaaaca cagctgcaaa gtctgttgga tcaaattgtc caagtacttg
    40141 ccgaagaagt ttctaatgaa atcaaagtag atgcaggtag cccgtttttt gaaacgaaaa
    40201 aacactttgg atgcggaggt aattgtcatg caattagaca atagtgaatt tgaaataaca
    40261 aaacgacgcg ggctgatcgt atgggtctac agcttaaaac aattaaagaa tctgaaaaag
    40321 ttcggcttgt tgcattatgt ctcaagaaga atgaaatatg tcgtgcttta cttaaatgaa
    40381 gagacattgg aagaaacaga agaacgaatc cgtaaattac attttgttcg aaacgtagaa
    40441 cgttcttacc gaccggatat cgaaatgaat tttgccgaaa aaataggaaa caaaacagta
    40501 ttaaaagacg aaggtttcga aattgaagag ctaaatacag aaatccgatt agctgaacat
    40561 ctttaaaaat ctcttatgag cagtttttaa ataaaaaata acttggataa cgatcgattt
    40621 ttgatcattg tccaagtatt tttttggtat actagtacca gtaccatttc taaggagtga
    40681 gtagatgcgg ataatatcag gaaattatgg cgggcgccgt ttaaaaagtt tagcaggggc
    40741 gaacaccaga ccaactacag ataaagtaaa agaatccata ttcaatatga ttggaccata
    40801 ttttgatgga gagacagtat tggatctgtt tgcaggaagc ggtggtttag ctatcgaagc
    40861 agtatctaga ggctgcagtc atgcggtatg cgtagacaaa aattaccaag cgctaaaaat
    40921 catcaaagaa aacatcgaaa ttacgaaaga accagagaaa tttaccacaa taaaacttga
    40981 tgcggacaaa gcaatcgaac ggctagcagc ggagaaacaa caattcgact acttgtttct
    41041 tgatccgcca tatgcaaaac aaaaaataat cgatcaaatc cataaaatgg aagaactggg
    41101 acttttttct aaaaccgcag ttatcgtatg cgaaacggat aaaacagtcg aattgccaga
    41161 aactgtggct ggattccatc aaataagaaa acagatttat ggcataacgg ccgtaactat
    41221 ctatagaaaa gagggaaatc aatgaaacgt gcattattcc caggaagttt tgatcctttt
    41281 acaaaaggtc acttagatac ggtggaacgt gcggctaaat tattcgatga ggtagtaatt
    41341 ggtgttttta tcaatacttc aaagaaaagt ttgtttcccc cagaagaaag aatgacattg
    41401 atcacaaaag ctgtttctca tcttcctaat gtaaaagtca tgcatcaaga aaatcaattg
    41461 acggtagaaa cagcaaaaga gattggtgca gatgcgttga tcagaggcat acgaagtatc
    41521 aaagactttg aatatgaacg agagattgct cagatgaatc atcatctata tgatgaattg
    41581 gaaactgtct tcttattagc aaaaccagaa tatagtcacg tgagttccag tattttaaaa
    41641 gaagtactgc actttggcgg agatgtgagc agctacttac cgccagtgat caacgaagct
    41701 ttagctggaa agcgtgaatc aaatgaaagc taaacaatgg ttgaaaacag tactgattat
    41761 catatttgga atgttgatca ttggatttgt agtagttccc ttgccgtatt atgtagaatc
    41821 accgggagcc acgatcgatc tgaaagaact aattacggtc aatgataagg aagataaagg
    41881 ttctgggtct ttctctctta cctctgttgg tatcaggcaa gctacaggtt tcttagcttt
    41941 aaaagctaaa atgacccctt ttgaagaatt gatcagtgaa gaagaattga ctggtggggc
    42001 gacaaatgaa gaatataatc aaatgcaaca gtattatatg gattcttccc aaaatgcagc
    42061 aatcgaacaa gcgttaaaac ttgcagacaa gccttataaa atggaattca aaggcgtata
    42121 tgtcatgagt gtagagaaaa catcgaattt ctttggcaag ctttcagttg gagatacagt
    42181 aacaaaagta gacgggaaaa gctttcaaag tacagaagca tttatggatt atgtaaaaag
    42241 ccaaaaagtt ggacaaacaa tcgaaatcag ttatttgcat aatggtgaag aaaaaacagc
    42301 ttctggtgat ctgatcgagt taccaacaga taaaaaggca ggaataggta tcacgcttac
    42361 tgaccatacc gagatcgaat cagatacaga tgttcgtatt aattctggct ctatcggtgg
    42421 gccatcagcg ggattgatgt tcactttaga aatctatgaa caagtcactg gtaaaaactt
    42481 gcgacatggt aagcaaattg caggaactgg aacgatcaac agtgaaggag aagttggccg
    42541 aatcggagga atcgataaaa aagtggcaag tgcggataaa gctggcgtag agattttctt
    42601 tgcaccagat gatacgattt cagacgatgt taagaaagaa tatcctgaaa taaaaaccaa
    42661 ctacgaagaa gcaaaagctg ccgctaaaaa attgaattcc tcaatgaaaa tcgtaccagt
    42721 aaaaacagta caagaagcgc ttgactatct agagaaaatg aaatgaaact aaataattaa
    42781 agaggggttg tgacacgagt tctgtcacaa cccctctttt tcgtcaaaaa tcttcacgat
    42841 aaaaataaaa acaaaagaaa aaattgacgt aattagatgt tgaagggacg attttgccag
    42901 agctcactgt ttcttcaatt ttaaatgaaa ggagtaatga atgattttta ctggtcattc
    42961 tttgaaaaaa gaacaatgat agaaaaaatc agcaagaaaa tactagcttc acccaaattg
    43021 atcatttgga tgtctatgat agttcttatc ttttgtatcg caagtggttt tttattatca
    43081 aatttgctat tttatagaga aacagaagag actgctgcaa aagaaatact ttcttcgttt
    43141 agttccagtg gagaagatcc atcagctgaa ggaaacgatg tagaagagga tgctcgtcca
    43201 aaggtcatgt acaccgatat caaaggaagt gtgaaggagc ctggtatcta ttccttttct
    43261 tcagaagaac gggtctatga tgtactaaag cgtgcaggtg gattattaga agaagcggat
    43321 agcgatcgaa tcaacttttc tgcgaagata gaagatcagc aagtcctata tatccctgct
    43381 gtaggtgaag agctgccaga acatcttgat cagttggcat cctcggaagg aaaacagcct
    43441 acagcagatt ccgagcctcc caaaataaat atcaatacag ccactccatc agaacttcag
    43501 cagattcctg ggatcggatc agtcaaagcg caagaaatca ttcgatttag agaagaaaac
    43561 ggatccttcc agaaagtgga agacctgcag gagatttctg gtatcggtga aaagacagtt
    43621 gaaaaactaa aaaacttcgt tacaattaaa tagaatgatt caaaatcgac tggcacgagc
    43681 aataaatgga cgctaatgga tactaagaat aatccaattc taagagcttg gcaggacgag
    43741 gaggtagtat ttttggatag gaaatagctt gattgttcgc aaagaaacat taaaaaaacc
    43801 aatgctgaat gtctatctcc aaaataaaat atctgggatt catattatga atacggctgt
    43861 cagcggcaat aacagtcaag ctctacgaga acgctttgcc aaagatgtac ttagctatac
    43921 agcagataaa gtatttattc tcattgggac gaatgatcta gcagagcata aacaactttc
    43981 taaagaaacg taccaaaaaa tctgcagtgg atgatagttc agctgaaaaa agactatagt
    44041 tctgatcatg ctttctttat cacgccacca gcagtcgatg agacaaaaca aaaatatcgg
    44101 aataacaagt tgatcaaaga atatatctct gttctcacag aagtagcaaa acgccatgat
    44161 tgtgaagtat cgatttttat acggaatttc ttgaacaaat ggattcgaag aatttttata
    44221 atgggctaat ggatgacgga cttcatttcg gcagtgaagg gtaggatctt ttgtcagaca
    44281 ggatgattcg ttatatttaa agaatatttt gtttaagcaa tgagtacttt ttaatcaact
    44341 tgaaaaaacg gaggaatata aaacatgtta gaaaaatctt caaaagcatg cgatacgaat
    44401 aaagaaaaag ttactgataa gtcacataat gaacgcattc cttgggacca gtactttatg
    44461 gctcaggcag tactgttatc tttacgtagt acgtgtacac gtttagaagt aggagcgaca
    44521 ctcgttcgag aaaaaaggat cattgctggc ggctataatg gagcagtcag tggagatgtc
    44581 cactgcatag atgaagggtg ttacgtcgtg gatggacact gcttgcgaac gatccatgca
    44641 gagatgaatg cactcctaca gtgtgcaaaa cttggtatcc cgacggaagg ttcagaaatc
    44701 tatgtcacac attttccatg tttggcctgt acaaaggctt tgttgcaagc tggtgtgaaa
    44761 gggatcaatt atctccatga ctatcgaaat gatccttatg cccaagcgtt gatcgaacaa
    44821 ttaggtgtat ctgtacacca agttgcttta gactcacagc atttttcaaa acttcaaaat
    44881 gaattaacag gaaaacatgt ctagcgtttt agaaaggcat gttcgaaatc ggttgatttt
    44941 tccggcaatt acgtcgatga ttatctgttt gttgtattac actgacaatt ggggttatcg
    45001 tttgcttctt gccggaattt ttttatggtt ttcgctaaaa gaaggacgtt ttatcgcgta
    45061 cttgtgtatt ttgtgtagca taacggtatc catttcttat aggctacaag agtattcatt
    45121 tttagaggaa aaaacaacat ctgcgcaaat tactgtattt acagatacga ttcgtgcaaa
    45181 tggtaatttt ttgacaatgg aaggaaggtt atcagagaat aaacaaaagc tccagctttc
    45241 ttatcaagta aacacagaag cagaattgaa aacatggctt aacttccgag gaaagggaat
    45301 agaattgact gctgaaggcg agttcatctc tccagcaaaa aagcggaatc tttataattt
    45361 tgaccaagaa gcttacttca ggaaccatag aatttctgga cggttccaaa ttcaaaagat
    45421 tgaacagatc accatgcaaa atcactggcg aaactggctt cagagaaaaa gaggatcttt
    45481 tatctccttt gtccaatctg aatttccgca aaaaacaagt gtatatatta attctttatt
    45541 atggggctat aaagatgcag gattcagaga accagaagaa atttttaggg agagtggttt
    45601 gctgcatctg ttcagtctat cgggtctaca catccaattt tatttagggt ggctgtatta
    45661 tttgtttcga cgtatcggat ggccactaca cgtcagtatt attccgttga gtctactaac
    45721 tatctgttat gtttctattg caggaagttc tatttctgtt ttgcgtgcta gtttgctgtt
    45781 tttattgcga ttgctagtca aattgctgaa taagcattat tccaccatgg acttatttgc
    45841 agttgtcttt tggcttcttt tactatttga tcctcgattt ctttttcatg cgggtggaca
    45901 gcttagtttt ttcatgagtt ttttgtttat cctgatttct tttgatggag attggttgaa
    45961 aaaaacggcc tctcacgtcc tgtttactat gatcacgatg ccgttgattg tatggcattt
    46021 ttatgagtgg cctattttag gtagtctttt tacattactg attgcaccgg tattcaagtt
    46081 tctttttctt cctggtgtgt tatttctcac actcttcaat cgctggctgc cggaaacttt
    46141 acttttgctg atagaagagg gattaatctt atttgagcga ataatcgatt tttcttcagg
    46201 gtcgtctctc attataggac gtttgcccct tgtgctatct gggatgtata ttttcggatt
    46261 actgatttgt atggataggt tgaaagaaca acctgtgaaa cacttgtttt atgttggtct
    46321 tttttctttt atttttttga ctttgccatt tttacgcttt tcttcaacga tcgcttttat
    46381 cgatgtcggt caaggagaca gtatcttttt gcaaagtcct tttcgaaaag aaacgatttt
    46441 gatcgatact ggaggtcagt tacactttga aagagaaaag tgggcgcaag gtatgatacg
    46501 tcctcctgct gaaagaaata tcgttccata tctaaaaggt caggggataa gtgtaatcga
    46561 taaactcatc ttgacacacg acgatacgga tcatgtggga gaattaccca cgttatctca
    46621 gcattttact atcaaaaaaa tttatatcgg ttggggcgct ggacgaaatg agccattaca
    46681 aaggcattta tctgaagctc aaaaaaatgg tactgagatc attgaaatca aacatgggga
    46741 caggatcagc ggttattatg atctttatgt attgactcct tttgaaaagg gagagggaag
    46801 aaatgaagat tcgattggtc tatggatgga atataacaat aggcgctttt tatttttagg
    46861 agatttgaat caagcaatgg aaaaacaatt gcttttgatt tatcctaatc tgaaagccga
    46921 tgtagtcaaa ttaggtcatc acggtagccg tacgtcaagt aacacagatt ttctttccca
    46981 tattgaagca aaacatggga ttatatcctg cggcgtaaat aatcgttatg gtcacccgca
    47041 cagcgaagta ttggagacac tagaagaaaa tcagataaaa acatggcgaa cggatcaaca
    47101 aggaatgatc acctatcgct ggcatccagt ttttcatcct catgggctag tggaaacaat
    47161 gatagactag taattctcat ctgctcatgg taggataatt tttgaaagtg gtgagtaatt
    47221 tgagtataca agcagagtta caaaaaatac gtaaagaaaa acttgcatct tgttacttga
    47281 ttttaggaac agaaaagttt ctccaagatc aagttcggac agaaattgtc aaaaaaatcc
    47341 aattagaggg cgaagatgac ttaaattttt taagttttga catggaaaat gcaagtttag
    47401 atgaggtagt agctgaagca gagacgttgc cgtttttcgg agatcaacgg ttagtattcg
    47461 tagagaatcc ttatttttta acaggtgaaa aagtaaacaa tggaatcgaa caaaatactg
    47521 atttattgac ggcttatttg aaagaaccat tagaatcgac tgtattagta ttctttgcgc
    47581 catatgaaaa gttggatgaa cggaaaaaag taacgaaaca attgaaaaaa acagcaatta
    47641 tcatcaatgt ccaacagttg aatgaaaaag aagtacgtca atatctgatg aataccttag
    47701 agaattcctc aatcaaaatg gaacgaagtg caattgattt attcttgagg ttgaccgact
    47761 tagatttatc caaaatgatg agagagttac agaaattggt cctttatgga caaaatcaac
    47821 agcaaatcac ggtaagagaa gtagaagaat tagttcctaa aacgttagaa cataatattt
    47881 ttgacatgac ccaatatatt ttaaaaggga agacggaaca agctttgcgt ttatacgaag
    47941 atttagttat gcaaggggaa gaaacaatca aaattaatgc gattttgctt tctcaattac
    48001 gtttattttt acagacaaaa tttcttgtaa agattggtta tcagcaggca aatattgctg
    48061 aaacattgaa aatccatcca tatcgtgtaa aacttgccat gcaggaagta aaaaaattcg
    48121 atgaacattt gctggtacag ttgtttgatc aattagttga gatggattat caaatcaaaa
    48181 cgggacaaat cgaaaaagaa ttaagttttc aactattcgt tttgcgcaca gggcagttgt
    48241 taagaaatcg ataagaagaa agacgagggc aaagcatgcg ggaaggtcgt ttgtttttgt
    48301 cctttttttg acaaataaaa aaatcggccg aagccgattc ttttatttag cgattttttt
    48361 gcttaaacga gatttgtcgc ggttagcttt gtttttgtgg attagacctt ttgattcagc
    48421 catatcaata gctttaacag cttctttata taacgcatct acgttgtcag cacctgaagc
    48481 tacagcatct tcaaattttt tgattgcagt acgcatagcg cttgtttgag atgaattttt
    48541 cacattagcg ttttcgcttg tacgaacacg tttgattgca gattcaatgt ttggcattag
    48601 tttcacctcc gataacttaa gttcttacgc gtgttaccgc ataattcaac attcattatt
    48661 atacctaaag gcagcgtgct ttgcaatatg aacatgacaa gtttttttcc ttaacagtct
    48721 tttattagga ttttttttca gtaaaaacgg atagtacaag ctgaaaaaaa tcaaaaagac
    48781 gctattgtgg gaggcaatag cgtcaaacca aactagattt ttatttatct atttaaacaa
    48841 agtttgaatg ggaatgtttg gtactaagtt attatataac aaagagaacc gtttgcaaag
    48901 ttttttttga aaagggatac gattccctgt tgttctttgt ttttctatta agaaactgca
    48961 gttattttat aaccagtatc ctttgatcag taaaggctat tctggtgtac cgattatttt
    49021 aatgtattca tcgatatttt ttatcaacat tttttttatg accaactggt tccgacggtt
    49081 ccatttgcca cttacttcca gcacatattt atttaggggc aaacgtaaga tatattttgc
    49141 caaatagcga tctttaagca ggccattgat cacacggtcc tcaagctgca agttgatccc
    49201 agtcagcatc attggctgtt cttgtaaaac gtacgtatca attaaaagac cttcaaaagt
    49261 ttccacaagc gtcttccttt cttttcaaag taacctgaaa acaggcacta attcttccat
    49321 tttgatattc gttgaagtgg atgcgatcat tctttctttt gtccactgta atctagtata
    49381 acaaaaaaga gcagaagagc caaaaagaaa aaagagcatc gaattctata ggaagtctca
    49441 ttggcacggc ctaaattatt acaaaacatt gattaaaaaa agcccctgca tcgcaggagc
    49501 atcccctata tcgttttaat aggtcaatca aaaagcagta tcaaacacat tttgtatcaa
    49561 atcaggaaat aatagataag ctaagcccag aatcaaaagt accgctagaa gaaaccagcc
    49621 gattgttcct atgaaatact ttaatttttc gaacattgta tcacctcgtt gtcagtatat
    49681 cacggatcag taaattgggg gtgttaagaa tctcctaaaa tagacaaaat ttgttctatt
    49741 ttcctaaagc aaatacttgt aagatacagt aagtgatacc agaagcaatg accggtccgg
    49801 cggcgattcc tcttaagaag acaacaccta aaatcgtgcc gaaaacaagt gcaacagtga
    49861 tttgtggatc agaagcaatc aatcctacac ccttggaaga taagacagcc actagcccac
    49921 cgcaaaaaac agctacccag ccaatagggg atttaaaggc ttctaataga tcacgtagac
    49981 cgatttctcc tgttgcgata gggattagaa tagccgctgt gatgatcgtc accccaatat
    50041 tgattccttt gccttgaagc caagggaaaa aggaacggga ggctggtatg agcttcataa
    50101 tcaaaacaaa agctgccgct atcatcagac tttgattttt agctaaaatt gctacaagta
    50161 aaataattcc taaaaaaagc caactttcca tgaaatatat tcctcacttt cttcgaaaca
    50221 atcataacat gtttttagaa agttacaaaa gtaggaattc atccttcaag acctattcat
    50281 aagctgtttt tttcataaaa taaacaaaat cataaaaaaa cactttgctt atttgacaga
    50341 agacagccgc gctttgtata gtggacaaga cttacatttt tttgaagaat cagaaagatc
    50401 aggtaaaggt cttagtaaac gcaagccatt cagaataacc aatattgtgc ttccttcgtg
    50461 tcctacaact ccaagaggta gatttaaaaa ttgcaggaag ttagaagcaa tcaacaagag
    50521 aataacaaga atcgaaaaaa taatattttg tttgatgatc cgccgcattt ttaaagatag
    50581 ccgatgactg tagacaaatt tatttaagtc gcttttcatt aagacgatgt ctgcgatatc
    50641 catagcggca tctgtccctt gccccatagc gacacctaaa gaagcggctg ctaaagcagg
    50701 cgcatcattg actccatcac caaccatcgc atttacttgg taaagttcat tctctttttt
    50761 gactagttgt gttttttctt ccggagtaca gcttgcatag taatgttcaa tccctacttg
    50821 ttcagcaatg gcctttgctg tgccagaatg gtcgccggtt atcatacttg tatggatgtt
    50881 ttgggattta aagtaggaaa ttacttgttg ggtatttgct tttggaatat ctagcagacc
    50941 taaaacagca attaactgct gatctttaga aagataaatc acggttttgc cttgattttc
    51001 caaacgttcg atcatttccg caatctcttt gctgattatc atcgtctcag gatcatatgc
    51061 gtgcttgcca actttccatt gcgcacctaa ataggtagtt tgtaatccaa aaccagtaat
    51121 ttcttctatt ggtaagtatt ggatttcttc aggtattgtg caatcgaaat attgcaaaat
    51181 cgcatgagca agtggatgcg tcgttttttg ttccatagca acaagaagtt gttgtgccaa
    51241 atccttttca tcaaagaaaa agctgtcagt tacaactggt tttcctttag tgagcgttcc
    51301 tgttttatca aaggcaatcg cttttaattc agaaagcttt tctaaatgga taccgccttt
    51361 gattaaaatc ccattttttg cagaatttga aagagcagct agagaagctg gtgtagcaga
    51421 tgcgacaagc gcacaaggag aagccacaac taataatacc attcctcgat aaaagctttc
    51481 atcccaactc caacctagga aaaagtgggg gagaaggatc attagaggaa cagcaagcaa
    51541 aatcaatttt acataattat tttcgattcg ttcgataaat gaagctgttt gagttggcgt
    51601 attctgcgct tcttctacta attggataat cttagcaaag acagtttcgg aactcgtttt
    51661 tgttactcga atcgtgatgg gatttccttg attcagcgtt cctgcataca tctcatcgcc
    51721 taaatatttt tctacaggca cagattcacc gcttaatgca gactcatcga tcgttgcctg
    51781 tgtgctttct aaaaagccat ctatcggaac agctgctcct ttagcgacaa ataccaagtt
    51841 atccaccttt agttgagaaa catctacttc ttgcatttgt ccattttctt ttaataattg
    51901 ggctttttgt ggttgaagat tcatcaaagc agtaatttct ttttgacttt tattagttgt
    51961 gtattcctct aatgcaccgc ttaaacaaaa gatgaatgtc aacattgctc cttcaaagta
    52021 attaccgatc aaacaggcac caatagcagc taaagccatc aataaatcca cattcagtgt
    52081 ccgatcattc cacaattctt tcaagccttc cgatgtttgt ttatatccac cgatgataat
    52141 ggcactgata taaaaaagtg gggtgatggt aagagagaat cggtcgaaga taaaaccgaa
    52201 gatcataaat aaaaggcagc cgattgttgc tgcaaaagct tttttctgat tagtcatcgt
    52261 tattagacct ccttgtcaaa acaaggatag cataataaaa atgtaattga taataataac
    52321 agctaaatga gaacgataat ggttatcatt tggtgattaa gcttgatgtt gtttttatta
    52381 tttataatga ttattattaa agaaaataaa aaaccagaac atttctcaaa taagagaagt
    52441 gttctggttt tttctacatt gcaataaacc aataaccaat caaaatgata ccaaggataa
    52501 tacgatacca gccaaaagcg gtaaagtcgt ttttcttcaa atagcttagt aagaatttga
    52561 tagctaagac ggaaaccaca aaagatacga atgttcctac aagaagaaca acagtttctt
    52621 ccattttgaa tgaattccct tcagctaaat atttgacgat cttcaagccg cttgcgccaa
    52681 acatgacagg aatccctaag aaaaatgaaa actctgtagc gacaaaacgc gaagcaccaa
    52741 tcaagataga tcctaggatc gttgcgccag aacgggatgt tccgggaaca agtgccaaga
    52801 cttggaataa cccaacaaga agagctgctt ggtacgtaaa gtcattcaga gaagtccaac
    52861 gaggtgtttt gtttttgttg cgtttttcga caataataaa ggcaataccg taaacgatca
    52921 aaacaatggc aactggtaag aacttgtgga aatttgcttc taaccaatca tccaaaggca
    52981 aaccaatgat gatggctggt atgcaagcaa cgatcacttt aaaccaaagt gtccacgtat
    53041 cttttttttc taccgttgat ttacttggag aaaatgggtt taacttgtga aaataaagaa
    53101 cgacgacagc caagatcgcc cctaattgga taacgacatt gaacatcgac ataaattgtg
    53161 aactggcatc cagtttgatg aattcatctg ctaggatcaa atgaccagta ctactgatgg
    53221 gcagccattc cgttattcct tcaataatac cgagaatcac tgctttgata atatttgcaa
    53281 aaaacataaa ttccttcctt cccaattaag atacatctta tagtataccg ttattttttg
    53341 agaaacccaa tctgtttttt aatattaaga aaaacttgag aaataaagca gataagtaca
    53401 cgaagagaaa aaagaagaca aagccctcgt tcagactttg ccttttatga ttctctggta
    53461 aaaattaaaa tatccccatt atagaaacga gtcgcgacta tgtgttttcc cgttatcgat
    53521 ataagacgta tcattgatgc cgtggataga aaatcgaccg tttgcataag tgacctttgt
    53581 tacgctgcca tttaaaagcc cagcacgaac aggctgttta ggatcgatca gattaagcaa
    53641 aaaggcgatc gtcaaaccat gagatacgac taaaacattt ccaccaccat ttttttcgca
    53701 gtgatgggcg atatcttcaa atcctgtcca tacacggtct cgcaatgttt cataagtttc
    53761 cgcccaccca gcggtatcta agttgtatac agcattagca atttgttcaa aagaaacttc
    53821 tgtggtaaac atttcatcat atgttttaaa attcaataca cgagggatca caccccacat
    53881 ttccatgtca tatccacctt caagagaccc gaaacaccat tctctaatcc ttttttcaat
    53941 aaaataagga atttcttttc ccttagaatg ttctcctaga ataatacggg cagtttcgat
    54001 tgcacgacca ctgtcactag aataagcaaa tgaaaagtcg acatttttta acccgcgacc
    54061 taaataatga atgccttctt gtccttgctt tgtgaggggt gtatcacacc agccttgtac
    54121 tcgttcaatt gtattgaaca tcgtttttcc gtgtcgtacg atataaagtg tagtttctgt
    54181 cattttattc tcctcctaag tcgttttaac gattgaaaaa aggatttgtc cttttttcct
    54241 gttcaatcgt cgttgcatcg ccatgtcctg gatagactgc aaattcatca ggtaatacaa
    54301 acagctgggt ccgaatacta tgaagtaatt gttctaaatt ccctgtatac agatcggttc
    54361 taccgatact tcctctaaac agggcgtcgc cagaaacgac aaaatcatca aaaataaagc
    54421 tgacgcttcc tatagaatgc cctggggtag ggacgacgcg aaaagacatg tttcctaaag
    54481 tataagtttt catttcaaat tcatattctg caggcgagac aataacatca gcaatatcat
    54541 catgtctacc gagtccagaa agattcaaaa tcggatcacc tagccatttt tgctctagtg
    54601 ggctgacata aactgggatt tgataatacg cgcgtaaagg ttccactgca ccgatatgat
    54661 cgtagtgagt atgggtaagc aaaatcgcta ccggctgttg gtttgttttt ttgatttctt
    54721 gtttgattcg ttccgcatct tctccagggt caatgatcaa aagtgcttct tcgttgtaga
    54781 ccaaataaca gttttcttca atcattcctg tttttaatct ttcgatatgc atgtgcattc
    54841 ctccttgata gccaaaactt ctttcatcca ttagtataac gcattcattg ctaggaaaca
    54901 aaaacaggta cttatttctc tttacttatc tgaattatca gactatatga aaagtatttg
    54961 gatattctaa agagaaggaa agtaagtttt catagcattt taatatgtcc ttgttacatt
    55021 agattcatcc aaaaatccaa attctatatc ctatatccct gcctaatcag cagggtgttt
    55081 ttttatgttt ggaaagagag ctctttttgc taaactaggt tttttcaggt aatcttagag
    55141 agaaagaggt ggtggaaatg atcaaacagt caaaaatatc tgtgcggcac ttagtagaat
    55201 ttattttgcg acgtggaagt atcgacaatc gtaaaaaaag taaccatact gcattagaag
    55261 gagcgagaat ccaccgcaag ctccaaaaag atgcgggaga aaactatgaa aaagaagtct
    55321 ttttaaaaac aacagttcaa ttagatgaat attacctgat cgttgaaggc cgtgcagacg
    55381 gtattttcaa aagagacggt atttattata tagatgaaat caaaacctcc gaaccacgat
    55441 ttgaagattt agaaccagaa caagtggaac ttttctttca tcaagcaagg gtgtatgcct
    55501 atatctatag tcaagaacat gatttacaag aaataaatct gcaattgact tacttccaga
    55561 cggcagaaga agtgatcacg agaaaggttg aacatcagac aagagagcag ttagaaactt
    55621 tttttaaaaa gctgactgaa gattataaag aatggcttgt ttttcaagaa aactggcgca
    55681 ctgtacgcaa tgcctctttg atggctttga aattccccca tgatgagtat aggaaaggtc
    55741 agcgtgaact agcggtagct gcttataaaa cgatccgtac gaaacagaaa ttgtttgtag
    55801 aagcacctac cggaacagga aaaacaatct ctacgttatt ccccgctttg aaagctgtag
    55861 gggaagaaga gggagaaaag atcttttatc taacggctaa aacaataact agacaagtag
    55921 ctgaagatgc tatgacagca ttaaaagata caggagctga agtaaaaagt gtcactttga
    55981 cggcgaaaga caagatctgc tttttagatg agacgacctg taatccagat cagtgcccat
    56041 atgccaatgg ttattacaat cggattaatg aaggtttatg ggatcttttg aatcatgaga
    56101 atcagatcac tcgtgaagtc atcgaaactt atgcaagaaa gcatacactt tgtcctttcg
    56161 aactatcatt agatgtcagt atctggtgtg acgtaatcat tggggattac aattatttat
    56221 ttgatccaac tgtttattta agacgttttt ttgaagatga aaaaaacgag gactatctgt
    56281 ttttgattga cgaagcacat aatttggtga atcgttcccg agagatgtat tcggcagaac
    56341 tatcttatga aaaaacgaaa cgaagtaagg aagctatacc aaaggaattc aaaaaacttc
    56401 atcgccgttt caacaaacta ttaaaagaat ttgattcgat ccgagaaata gcaaaagaag
    56461 atcattggga ttatcatcat caaaaagcac cggctgaatc tttagtaaag gcaggttatc
    56521 aattaagcga aaaaattaaa gaatggctgg cagaatttcc tgagcaccca tcacaagaac
    56581 aattgcttcc ttattatttt gatttgcttc attttttgaa ggtcagtgaa ttttatgatg
    56641 atcactacga aacaacagtc gaaaaaacgt atcgcgatct gatagtaaaa gagttttgta
    56701 ttgatccaag cttgtttctt gagcagtcgt tggataaagg aaaaagcagt attctttttt
    56761 ctgctagttt ttcaccactc tcttattatc aggaaacact ggggggacaa aaaagtctgg
    56821 cttacaaact gccgagtcct ttcccagaag gaaaccagca agttttcatc gcaaattacc
    56881 tagagacgac ttatcgaaaa agggaacaca gtctggcaaa attagtagag acgattcatg
    56941 cttttgctga tggaaaggtc gggaattatt tcgttttctt tccttcttat caatatttag
    57001 atcttgcggt ggaagcattc agacagcaat atcccgacag caatgtgcta atccaagaaa
    57061 cggacatgaa tgaggaagaa cgcgaacgat ttttaacaaa atttcaaaca aatcccgctg
    57121 aaacattgat tggattttgc gtacttggcg ggattttttc agaaggaatc gatttgagag
    57181 gggaccgatt gatcggcagt atgatcgtag gtgtaggttt gccgcagatg aatcatgagc
    57241 aggaactgat caaatcatat tatgatgaga aggaacacct tggatttgct tacgcttatc
    57301 agcttcctgg aatgaacaaa gtcttgcaag ctggtggtcg agtgattcgt ggcatggcgg
    57361 atcaaggaat cgttgtattg gcagatcaac gattttctag tttttcttat cgacgtttat
    57421 tcccacaaca ttggcaaaca gcaagagaag tacgaagtgt tggggaattg gaagaaggaa
    57481 tacagcgttt ttggttatca aaagaagcag taaatcaaaa aaacgaggaa tgacagacgt
    57541 cagcctcgtt ttttgattaa cgatagttag tgaattgcaa ctcgattggt aagtcaagtt
    57601 tacgtaataa agcaatcaca gcttgtagat catcacggtt cttacctgtc actctaatct
    57661 gatcttcttg gatttgtgct tttactttca gtttttcatt tttgatagct gtagtgattt
    57721 tcttcgcgtt ttcacgatcg attccgttga ccaaatcggc agtttgtcga gctttgccac
    57781 ctagagcgtg ttctgattcg gaaaaatgga tatttttgat tggcacattt cttttgatca
    57841 gcttaccaaa taatacatct ttgatctgtt cgattttgaa atcatcatcg ctcaatacaa
    57901 ctagattgct gttctctaat ttgatttcta ctgtagaatc cttgaagtca aagcgattgg
    57961 acaattcttt tgttgtttgc tgaatcgcat tttttacttc ttctgtattg acttctgata
    58021 caatgtcgaa gctagcatct tttgccatag gaaccctcct gatatacata taaatattta
    58081 ccatacatct atcttatcat aggattaggg cagattctta cctgtttgct aaaattcttc
    58141 tcatttgagt tcgaagtatt ttattttaat agataaactg ttattcttac atttggaata
    58201 ccaaagtagg ggagaggtgc ttgtgagtaa aattatgact gcgtatcata aaaatgaaag
    58261 attaaagaaa atagtggtta ttttgataac cggattgacc gctgcggtcg ctttaaatta
    58321 ttttttgatt ccagccaatg tcttttctgc agggatgaat gggatcgctc aaatcatttc
    58381 ctcgctttta tatgaatggt ttcatatcaa gatagacaca ggaacattta tttttctttt
    58441 gaatatccct gtgtttgtat tagggtttct caaagtagga aaacgagcaa cgatccttag
    58501 ttttgtgaat gttgtatgtg tatcggtgat gacgatgctg cttccagtcg gacagatcac
    58561 tgataatatt ttgatgaatg ccctagtagg cggtgtttta ttaggtcttg gcgtaggatt
    58621 atctttaaaa atgggcttta caactggtgg gatggatatc atctctcttg tactttcaca
    58681 aacaactgga aaaacagtcg gaaactacat gctgattcta aatggtatca ttgtagtcat
    58741 cgcaggcttc ttctttaact gggaaagtgc cttgtatacg attatttcca tttatgcaat
    58801 gactcaagta attgatgcca tccacacgag tcatcaaaaa gtaacagcaa tgatcgttac
    58861 cgtcaagcca gaagaggtaa cagaagaact agcaaaacgg atggtacgtg gaatgacatt
    58921 actgccgtct gtcggtggct attcaaaaaa acaagggaac atgatcatga tggtcatcac
    58981 ccgctacgaa atgtatgatc ttgatcagat cgttcatgaa atcgacgacg atgcatttat
    59041 caatatttta ccaactcatt cagtatttgg ccggtttgca aatgaaaacg aacaaaaaat
    59101 gtatcgaaca actggcgtat tgccagaaat aaaaaatcgt tcacaaaaaa aatgaggaat
    59161 caggtttatt ttagaataaa tttaatctat tacatctaat aagaacattt ttataaattt
    59221 aaacgagaat cttacttcta aattctcatt tacaagcagc taaaaaaaga gtatgataaa
    59281 taaaaatcgt gttaaggagt gtcgtacgat gagcgaagca aaccaagaac ggtctggaca
    59341 agaaatagaa ctaatcaaag aacgtattct tgctgccttg gaaatggtga ttgatcctga
    59401 attaggaatc gacatcgtca atttaggctt gatttatgat atagaattca atcctgaaaa
    59461 tggagaaaca gtcattaaga tgactctaac aactatgggc tgtccattag cagatatttt
    59521 aacagaatct atccatgacg cattaaaaga agtgccagaa gtaacgaaag cagaagtcaa
    59581 actagtctgg tatccagcat ggacgacaga taagatgagc cgttatgcac ggattgcgtt
    59641 ggggatacga tagaaatctt ggctgactag ctatgagaag caaataggaa aaacaaaaaa
    59701 gccttttatc ctttctagaa gaaaagagat aaaaggtttc tttgtttata aaattttgtc
    59761 tgctatatta taagttaatt aaataaatgt gttctattga taaaggacat tcaaatccct
    59821 ttccttttgg aatccttctt ttttacatca gtttgatttt cgtatagtat tctttctcat
    59881 cttcacgata aatcgcagcc ccattttttt gttgtgcttt aagactgcca agattttttt
    59941 tatttacaga caaaagtgcc tcatcaatgc cgattttttt tgcctcttgt agtactaatg
    60001 ctaatccttg tgtggcatat ccttttttac gataattagg ggagatgccg taaccaatat
    60061 ggccagcgcc gtttcttaat gtttcgttca gtttatggcg caaattaaaa attcctacat
    60121 actcatgttc atcactttct aaaataaaga cggttgcggg aacgtaactc tcaggaagat
    60181 cgattcctaa agacgttttt tcgcattttt ttacataatc taaaaattca gcaaatgtat
    60241 agccataagc attgttgata aatccactct catttggtcc aaagctagtt tgtagctgat
    60301 aagctttctt taaatctgct tcccatagtt tgatcagttt catcgtattc acctctttaa
    60361 gtaaccttat tcatttgttt tctactatag cactattgtt gtaaatagcc atgcctattt
    60421 ttatttattg tgtcatatcg tattaagaat catcgaacaa tacaaatatg aattacaata
    60481 aaaaaattat ttgaagttcg ttcattcgtt acttatgatc tctagaagaa agaaaacgtg
    60541 aattacttca gcgtctagaa ttccagaagg atagtaatca acagaagaaa taatcaatat
    60601 ccaaatgaaa gaagatagct gaatcgagaa aaagaggaaa aaagaaatga agaaaaaaat
    60661 aaaatagtta ctaatcccag agtcgttcgt ttaacattga aatcgatcat atggataaaa
    60721 cagatttttt caccaaccct caatagatga tggagttgct cggatgaatt atttgtgtag
    60781 atggcataag ggagggcgta cagaaatgaa aaagaacaaa aatatgttct tgacaaaaaa
    60841 cacatgtaat cctaaactaa aagatgtgat gcggctatgg cggaattggc agacgcgcta
    60901 gcttcaggcg ctagtgaggg tgacctcgtg gaagttcgag tcttcttagc cgcataattt
    60961 gtttttttat aaagagaagg tacacctgga gcgatgagaa agaaaataac atattatcta
    61021 aaagaatata ggaaaaattt taaacaacgg aaccagattc aaataaatca aaaaaaattt
    61081 gtgtgaaggt gggacagttt aaaacctaat agattgattc aaatagtaga tttatagatt
    61141 ctgatacaag aaatactggc aaacgatagt taaggaataa agactaaact taaactcaaa
    61201 aagaggtcat gcgaaagaat aaatcgcatg acctcttact atattttgaa tgtgtttaaa
    61261 cgttcaaata atgaatctta gtaccaaccg tttgcttccc agaatgcttt cgccgcttcc
    61321 caagaaccat aacgtgaagt aacatattgt tctgctactc tttcttggtt tgcagcagag
    61381 tagtcaccat ttaagtaaga agaatctaat tggtaacggc cgatgtaacg tccgttcgtt
    61441 gctgtgtaag aaccgcttga ttctttttgt gcgatccatt cttttgcaga gcttgtactt
    61501 gctggagtag cagcagtttc tgttacagca ggagcaggtt gtgtttcagc aacaggcgct
    61561 gcttgtgttt ccgtttgaac cgtttcttgt acaacaggtt gttcagcttg aacaggttca
    61621 gcttggactg gtgcttcttg aacggctgct tgaggttcag cagctgcttg tgcagagcct
    61681 tctgttggga ttgttaattg ttgtcctgag ta
//
LOCUS       DAYYGO010000004        57094 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000004 DAYYGO010000000
VERSION     DAYYGO010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57094
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.004"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(502..1005)
                     /locus_tag="ACHA5T_000194"
     CDS             complement(502..1005)
                     /locus_tag="ACHA5T_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM8885803.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1364..2389
                     /locus_tag="ACHA5T_000195"
     CDS             1364..2389
                     /locus_tag="ACHA5T_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM8885804.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2712..3590
                     /locus_tag="ACHA5T_000196"
     CDS             2712..3590
                     /locus_tag="ACHA5T_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HFM8885805.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            4066..4182
                     /locus_tag="ACHA5T_000197"
     CDS             4066..4182
                     /locus_tag="ACHA5T_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFM8885806.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            5110..5448
                     /locus_tag="ACHA5T_000198"
     CDS             5110..5448
                     /locus_tag="ACHA5T_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8885807.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5542..5772
                     /locus_tag="ACHA5T_000199"
     CDS             5542..5772
                     /locus_tag="ACHA5T_000199"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFM8885808.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            6172..6543
                     /locus_tag="ACHA5T_000200"
     CDS             6172..6543
                     /locus_tag="ACHA5T_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFM8885809.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(6817..7509)
                     /locus_tag="ACHA5T_000201"
     CDS             complement(6817..7509)
                     /locus_tag="ACHA5T_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8885810.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(7512..8297)
                     /locus_tag="ACHA5T_000202"
     CDS             complement(7512..8297)
                     /locus_tag="ACHA5T_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8885811.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            8534..9934
                     /gene="lacG"
                     /locus_tag="ACHA5T_000203"
     CDS             8534..9934
                     /gene="lacG"
                     /locus_tag="ACHA5T_000203"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFM8885812.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            9959..10276
                     /locus_tag="ACHA5T_000204"
     CDS             9959..10276
                     /locus_tag="ACHA5T_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM8885813.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            10289..11962
                     /locus_tag="ACHA5T_000205"
     CDS             10289..11962
                     /locus_tag="ACHA5T_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFM8885814.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            11976..13607
                     /locus_tag="ACHA5T_000206"
     CDS             11976..13607
                     /locus_tag="ACHA5T_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFM8885815.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(14098..15663)
                     /gene="guaA"
                     /locus_tag="ACHA5T_000207"
     CDS             complement(14098..15663)
                     /gene="guaA"
                     /locus_tag="ACHA5T_000207"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFM8885816.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(15722..16645)
                     /gene="coaA"
                     /locus_tag="ACHA5T_000208"
     CDS             complement(15722..16645)
                     /gene="coaA"
                     /locus_tag="ACHA5T_000208"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFM8885817.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            16841..17446
                     /locus_tag="ACHA5T_000209"
     CDS             16841..17446
                     /locus_tag="ACHA5T_000209"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8885818.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            17754..18770
                     /locus_tag="ACHA5T_000210"
     CDS             17754..18770
                     /locus_tag="ACHA5T_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8885819.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            18911..20212
                     /locus_tag="ACHA5T_000211"
     CDS             18911..20212
                     /locus_tag="ACHA5T_000211"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFM8885820.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            20258..20920
                     /gene="deoC"
                     /locus_tag="ACHA5T_000212"
     CDS             20258..20920
                     /gene="deoC"
                     /locus_tag="ACHA5T_000212"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFM8885821.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            20941..21339
                     /locus_tag="ACHA5T_000213"
     CDS             20941..21339
                     /locus_tag="ACHA5T_000213"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFM8885822.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            21405..22487
                     /locus_tag="ACHA5T_000214"
     CDS             21405..22487
                     /locus_tag="ACHA5T_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8885823.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            22699..24261
                     /locus_tag="ACHA5T_000215"
     CDS             22699..24261
                     /locus_tag="ACHA5T_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8885824.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            24254..25387
                     /locus_tag="ACHA5T_000216"
     CDS             24254..25387
                     /locus_tag="ACHA5T_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8885825.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            25387..26343
                     /locus_tag="ACHA5T_000217"
     CDS             25387..26343
                     /locus_tag="ACHA5T_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8885826.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            26442..27608
                     /gene="deoB"
                     /locus_tag="ACHA5T_000218"
     CDS             26442..27608
                     /gene="deoB"
                     /locus_tag="ACHA5T_000218"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFM8885827.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            27632..28450
                     /locus_tag="ACHA5T_000219"
     CDS             27632..28450
                     /locus_tag="ACHA5T_000219"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFM8885828.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            28467..29174
                     /gene="deoD"
                     /locus_tag="ACHA5T_000220"
     CDS             28467..29174
                     /gene="deoD"
                     /locus_tag="ACHA5T_000220"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFM8885829.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(29472..30158)
                     /gene="gpmA"
                     /locus_tag="ACHA5T_000221"
     CDS             complement(29472..30158)
                     /gene="gpmA"
                     /locus_tag="ACHA5T_000221"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFM8885830.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(30330..30911)
                     /locus_tag="ACHA5T_000222"
     CDS             complement(30330..30911)
                     /locus_tag="ACHA5T_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8885831.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(31044..31727)
                     /gene="rpiA"
                     /locus_tag="ACHA5T_000223"
     CDS             complement(31044..31727)
                     /gene="rpiA"
                     /locus_tag="ACHA5T_000223"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFM8885832.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            32046..32459
                     /gene="rpsL"
                     /locus_tag="ACHA5T_000224"
     CDS             32046..32459
                     /gene="rpsL"
                     /locus_tag="ACHA5T_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFM8885833.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            32543..33013
                     /gene="rpsG"
                     /locus_tag="ACHA5T_000225"
     CDS             32543..33013
                     /gene="rpsG"
                     /locus_tag="ACHA5T_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFM8885834.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            33107..35194
                     /gene="fusA"
                     /locus_tag="ACHA5T_000226"
     CDS             33107..35194
                     /gene="fusA"
                     /locus_tag="ACHA5T_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFM8885835.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            35366..36553
                     /gene="tuf"
                     /locus_tag="ACHA5T_000227"
     CDS             35366..36553
                     /gene="tuf"
                     /locus_tag="ACHA5T_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFM8885836.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            36960..37268
                     /gene="rpsJ"
                     /locus_tag="ACHA5T_000228"
     CDS             36960..37268
                     /gene="rpsJ"
                     /locus_tag="ACHA5T_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFM8885837.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            37299..37928
                     /gene="rplC"
                     /locus_tag="ACHA5T_000229"
     CDS             37299..37928
                     /gene="rplC"
                     /locus_tag="ACHA5T_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFM8885838.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            37956..38579
                     /gene="rplD"
                     /locus_tag="ACHA5T_000230"
     CDS             37956..38579
                     /gene="rplD"
                     /locus_tag="ACHA5T_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFM8885839.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            38579..38869
                     /gene="rplW"
                     /locus_tag="ACHA5T_000231"
     CDS             38579..38869
                     /gene="rplW"
                     /locus_tag="ACHA5T_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFM8885840.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            38908..39741
                     /gene="rplB"
                     /locus_tag="ACHA5T_000232"
     CDS             38908..39741
                     /gene="rplB"
                     /locus_tag="ACHA5T_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFM8885841.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            39784..40062
                     /gene="rpsS"
                     /locus_tag="ACHA5T_000233"
     CDS             39784..40062
                     /gene="rpsS"
                     /locus_tag="ACHA5T_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFM8885842.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            40085..40432
                     /gene="rplV"
                     /locus_tag="ACHA5T_000234"
     CDS             40085..40432
                     /gene="rplV"
                     /locus_tag="ACHA5T_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFM8885843.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            40446..41102
                     /gene="rpsC"
                     /locus_tag="ACHA5T_000235"
     CDS             40446..41102
                     /gene="rpsC"
                     /locus_tag="ACHA5T_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFM8885844.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            41105..41539
                     /gene="rplP"
                     /locus_tag="ACHA5T_000236"
     CDS             41105..41539
                     /gene="rplP"
                     /locus_tag="ACHA5T_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFM8885845.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            41529..41717
                     /gene="rpmC"
                     /locus_tag="ACHA5T_000237"
     CDS             41529..41717
                     /gene="rpmC"
                     /locus_tag="ACHA5T_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFM8885846.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            41742..42008
                     /gene="rpsQ"
                     /locus_tag="ACHA5T_000238"
     CDS             41742..42008
                     /gene="rpsQ"
                     /locus_tag="ACHA5T_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFM8885847.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            42066..42434
                     /gene="rplN"
                     /locus_tag="ACHA5T_000239"
     CDS             42066..42434
                     /gene="rplN"
                     /locus_tag="ACHA5T_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFM8885848.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            42471..42779
                     /gene="rplX"
                     /locus_tag="ACHA5T_000240"
     CDS             42471..42779
                     /gene="rplX"
                     /locus_tag="ACHA5T_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFM8885849.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            42807..43346
                     /gene="rplE"
                     /locus_tag="ACHA5T_000241"
     CDS             42807..43346
                     /gene="rplE"
                     /locus_tag="ACHA5T_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFM8885850.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            43365..43550
                     /locus_tag="ACHA5T_000242"
     CDS             43365..43550
                     /locus_tag="ACHA5T_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFM8885851.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            43586..43984
                     /gene="rpsH"
                     /locus_tag="ACHA5T_000243"
     CDS             43586..43984
                     /gene="rpsH"
                     /locus_tag="ACHA5T_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFM8885852.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            44016..44552
                     /gene="rplF"
                     /locus_tag="ACHA5T_000244"
     CDS             44016..44552
                     /gene="rplF"
                     /locus_tag="ACHA5T_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFM8885853.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            44713..45069
                     /gene="rplR"
                     /locus_tag="ACHA5T_000245"
     CDS             44713..45069
                     /gene="rplR"
                     /locus_tag="ACHA5T_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFM8885854.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            45090..45590
                     /gene="rpsE"
                     /locus_tag="ACHA5T_000246"
     CDS             45090..45590
                     /gene="rpsE"
                     /locus_tag="ACHA5T_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFM8885855.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            45605..45784
                     /gene="rpmD"
                     /locus_tag="ACHA5T_000247"
     CDS             45605..45784
                     /gene="rpmD"
                     /locus_tag="ACHA5T_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFM8885856.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            45828..46268
                     /gene="rplO"
                     /locus_tag="ACHA5T_000248"
     CDS             45828..46268
                     /gene="rplO"
                     /locus_tag="ACHA5T_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFM8885857.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            46268..47563
                     /gene="secY"
                     /locus_tag="ACHA5T_000249"
     CDS             46268..47563
                     /gene="secY"
                     /locus_tag="ACHA5T_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFM8885858.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            47625..48272
                     /locus_tag="ACHA5T_000250"
     CDS             47625..48272
                     /locus_tag="ACHA5T_000250"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFM8885859.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            48463..48681
                     /gene="infA"
                     /locus_tag="ACHA5T_000251"
     CDS             48463..48681
                     /gene="infA"
                     /locus_tag="ACHA5T_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFM8885860.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            48714..48830
                     /gene="rpmJ"
                     /locus_tag="ACHA5T_000252"
     CDS             48714..48830
                     /gene="rpmJ"
                     /locus_tag="ACHA5T_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFM8885861.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            48848..49213
                     /gene="rpsM"
                     /locus_tag="ACHA5T_000253"
     CDS             48848..49213
                     /gene="rpsM"
                     /locus_tag="ACHA5T_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFM8885862.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            49241..49630
                     /gene="rpsK"
                     /locus_tag="ACHA5T_000254"
     CDS             49241..49630
                     /gene="rpsK"
                     /locus_tag="ACHA5T_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFM8885863.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            49710..50648
                     /locus_tag="ACHA5T_000255"
     CDS             49710..50648
                     /locus_tag="ACHA5T_000255"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFM8885864.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            50676..51056
                     /gene="rplQ"
                     /locus_tag="ACHA5T_000256"
     CDS             50676..51056
                     /gene="rplQ"
                     /locus_tag="ACHA5T_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFM8885865.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            51328..52167
                     /locus_tag="ACHA5T_000257"
     CDS             51328..52167
                     /locus_tag="ACHA5T_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8885866.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            52143..53012
                     /locus_tag="ACHA5T_000258"
     CDS             52143..53012
                     /locus_tag="ACHA5T_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8885867.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            53002..53799
                     /locus_tag="ACHA5T_000259"
     CDS             53002..53799
                     /locus_tag="ACHA5T_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFM8885868.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            54002..54748
                     /gene="truA"
                     /locus_tag="ACHA5T_000260"
     CDS             54002..54748
                     /gene="truA"
                     /locus_tag="ACHA5T_000260"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFM8885869.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            55412..55606
                     /locus_tag="ACHA5T_000261"
                     /pseudo
     CDS             55412..55606
                     /locus_tag="ACHA5T_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(55794..56570)
                     /locus_tag="ACHA5T_000262"
     CDS             complement(55794..56570)
                     /locus_tag="ACHA5T_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8885870.1"
                     /translation="MNIHHIRNATAILDYGGKKFLIDPMLAEKGTLPPFISKGPFPSP
                     RKDQMNPLVDLPMPVEDIINGIDAVIVTHLHLDHWDDIAKEVLPKEIKLFVQDENDAN
                     EIRLYGFKNVEVLREDTVFESIQLVKTKGKHGRGEILNYVGNVCGIVFKHSTEKTLYF
                     TGDTVWYEAVKQTIDQYNPEIIVANAGDNHFLVGESLIMGKDDLYELHKAIPDSTIIA
                     VHMEAVNHWGLSREELKSFNKEKGISEKVLVPEDGDNIFI"
     gene            56693..>57094
                     /locus_tag="ACHA5T_000263"
     CDS             56693..>57094
                     /locus_tag="ACHA5T_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HFM8885871.1"
                     /translation="MLDQTDINIIEELSKNSRITMKELGEKVHLTGPAVSARVTKLEE
                     SGVIENYTVKLNMEKLGFSIHAFLIIITQNLNHKPYLSFIKNHEKFLIRNYKISGGGC
                     YLLECRFTSKKSMNSFLEQLNEYANYKLSIVI"
BASE COUNT        18256 a        10181 c        12172 g        16485 t
ORIGIN      
        1 tttgttattt tatacttaaa aaataacgac acagaaaata ccgtgtctca gatttctcta
       61 ctacttctaa aaactactgt ttttttcctt tgaaatgcct tcaaaaagta ggatcaggca
      121 gaatatactg gcagaatcaa ggagaaggga gcctaaataa ggagaaaaac catagaaaat
      181 ataaagtaat tacttataaa acaagctttt tatagaaaaa taatatacct gagtagggta
      241 taaattaaga cggaattcct ttctagattg agatattgac taggaagaat gttaagattt
      301 taagccttta agctaaaaaa aaaccctttt tgttaaataa ggggaatata gcctgtcagt
      361 ttcaaactgc aagtcgtcct gctgcaataa tatagtagat tagagatgaa aatagatttt
      421 tttaaaccaa tgactgaata aaaagaccgt catagataca cattggaatg tgtctattac
      481 ggtctttttt tacgaacgat tttattgaag acggacagtt cgggtcaaaa ataaaaaagc
      541 tacgatcaga aataagaata aaatgtatcc caaaataatg gctagacggt ctaacttatt
      601 tgtcgataga tgtttgttta gaagttgata ggcccataaa cctatcagtc ctcctgttgt
      661 attagttatc aaatcagtga tgtccgctac gccaataccg aataggaatt gaatcaattc
      721 tgccaagaag cttacaagga aaataatccc taatttttta gaaaaagaaa gtctcttgaa
      781 attgacattc aaaagcaccc caaaaggaaa aaagacaatc aagttataag tggtctcacg
      841 taataccgtc gtattttcaa aagaaaaagt agaaaaaggg acgagattga cattgctgta
      901 agaataagtg agaaattgtg gaagattaaa tgatagcttg aataaaatca accataatag
      961 aaagaataaa taaatcccta ataaaaattt tgatacatac cgcatagaca tctcctccat
     1021 ttaaaaagta taccatcgaa aaccgctgaa aaatcataaa aaagtatgaa gtttaaggac
     1081 caacgtagtt atcgtacgaa aattagaagc ttttttttgt tgggaatccg gtttctttaa
     1141 caaagctaag atagcgagtt gcttggaaaa gttgctatct tttttctgtc aaaacgactc
     1201 ggaatgttcg ttttttgttc taatttctcc aagttgttta ctttttgata ctaagtttat
     1261 gaaaaatcaa gaaatagttg ttatactagg taatgctgac aaacgggatt ggcatttcat
     1321 cttggttcag ctttttttcc aattactagg aaggagggat agattgttca agacttattt
     1381 tcgtttcaat aaaatacaag ataatccttt tcgtatcatg aatctgatgc tacttatctt
     1441 tattgtatta tggttaggtt acataaatca tgattttttg atttcaagtt tttcatcatt
     1501 agggatcttt acttttttct attatcaaaa cataccgatg aaatatttgc tgaaacgtat
     1561 gtttctgatc ggttgcggac tgttggttgc tttcgttttg gggatcttat cgacttatgt
     1621 tttatggctt gaaccatttg ccgtagcttt ggttgctttt tccagcagat tcgtgttgcg
     1681 attattccat atttctaaac caggtggctt gttttttgcg atgctttctg ccatgggaac
     1741 gagcatgcag ctgccaatcg ctcaattacc gatcgtctca ctttattttt ttatgggtgt
     1801 cgtttttgcc ctgattgcgg cagtgattac aaaattactg gattcacggc ctgagcagac
     1861 aattgaaaaa gcaactttaa aagaaagatt tcacgaagag cctttagtga tcattgattc
     1921 tgttttctac agcgctgcct tatttctttc cgtttatgtc agtcacggct tgaatctgca
     1981 taatccttat tggttgacct tgtcatgtgc atcgattttg ctagcagaaa acttggatgc
     2041 gatgaagcat cgacaagtcc aatatctgat tggatcgatg ggtgggttat gtgtgtcggc
     2101 attcttgtca tttgtgccgt ttacgcagtt gcagaccatt tttctgatca cttttctata
     2161 tggaatcgca caatttctag ttgctagaaa ttatgcagtt gctaatattt ttcttaatcc
     2221 gatggcgttg atgctatcta cacttattcg tggagcatac ttgatttctt tgatcgaata
     2281 tcgtttttta gggatcgtca taggaagttt catcggttta ggtgttgcgt gggtaatgac
     2341 ggttggtttg caacattatt taacggttgt tcgtcaaaaa ttagaataga aggacaaata
     2401 tatgtgccaa aagaggttat gacaagattt ttgtcacaac ctctaattca aggtgttcaa
     2461 aagccagatt ctcagcatta agtcaaattc acacaaaaat gtggaaagga actttcaaaa
     2521 aaattttata tttattctat caaaaaattt tgtttatgat ggtaactgaa taaactttcc
     2581 tgagatggtc aatgctttcc ttgaaagtct tacagatatt ctttcttcat agaaagttta
     2641 agttttatag ttagtgtaca ataaaagtat gtttatacca tatggtgtcc ggataatcag
     2701 tcatttaaac aatgaggttc aaagatatga aaaggggaaa tttgatgaag aaaataagca
     2761 atgaaatcca aattctggca gaaactttaa ctcttcccaa agtcgttata gagcaaatga
     2821 ttgtacaagc aaaaaacatc cttgatgagg tgtttgagca atatagtcat gaactaatgg
     2881 atcctaataa agcaaaggcg actgcaaaga aaatagacaa agtctatgaa gaggaacctt
     2941 ttgcagcatt agctatttat ctatatgcag cttctcaaag ttggaaaaaa atttatcaac
     3001 cattgaagat tccaagaact atctatttgg caacaatgaa tgcttttact agatttattc
     3061 atgaacattt ccaagtaaca ggaagctatc gctttgaccg tggtttttgg atttggcatt
     3121 acactagtgg cttgatcttt cggattggtg aattggagta cgaaatgaca tattttccga
     3181 aagggtacac ggatctaaga ttagaaggaa agcagtgcct ttctcttcat attccttccg
     3241 atgcagattt atctcagaat aagataagag atagttacga aagtgccatt cgtttttttg
     3301 aactttattt tcctgattac cattaccaag tgatgtacac agatacttgg ttactatcac
     3361 ccaatcttac taaatggttg aaaaaagagt caaaaatctg tctttttgca gccgattatc
     3421 gattattatc agttgatgaa caggacgata gtggagtccc ttggatcttt ggacgagtag
     3481 atgcccaaat acatgattat ccagaaagta cgagtttaca gcgacaagca aaagagcaat
     3541 tgcttgcagg cgagcacatt ggctctggct taggtattct tacttcataa gtttagtact
     3601 tcgaaatagt ttaaaagagc gtgagacaaa agtaaagaac acttttgtct cacgctctaa
     3661 acacgtataa acggcggaag cagaagcaac tccttcggaa ataagctgaa attcacaaaa
     3721 atttgaaaag caattttcgt gaatttcttc ttatttcttg gagttaaacg cttctgtccc
     3781 agcctctttt ttagtgttct atcgaacaca cagtacgttc aataggtaaa ctagctataa
     3841 caatagatat taaagttaac ggacaagagg gcctttttta ggtagctaaa acgtaataat
     3901 gaaaatttat ttccagagat aggagtcaat cagctagtct gatcgactgt tttaacaagt
     3961 tgactttcat tttaactttt gataccattt aaaacacaaa tcgtaattgt ttcgatttgt
     4021 gttttttagc atgactacaa tcaaagaaga aagtgggtcg tccatatgga aaaaacagat
     4081 atctcaagtg cataccgccg cttgaaaagc ccaaatatca aaacacgaaa acgtgcgctg
     4141 aagatcatca aagatgtgaa acggaatagt gggaaaaggt aagaagaagg aaacttaatt
     4201 aaaaaaatag ccgaaaaacg tttttcggct attttcaata atcggttttt gtgtaaggtt
     4261 tccgaaagaa ctgctgttga ttttccattt ttaacatatg ataaattggt agaatctttc
     4321 ttttcaaatt gataccattc cctaattcca gttatatagt taactacaga accacaaagc
     4381 acgtacgttt ataaaagtat tatctgtagt tatttttcaa ttccctttca acctcttatt
     4441 ttgaccagta gtattgatca ctttaccttt taacaccttt gttcgttttt gaaacatttt
     4501 tagtcaagaa tggaaacgta actacataat caaaggtttt gcgaaatatt tcatgaaata
     4561 tttcatgtag aagcgagcaa tacggttgca aataaccatt atcgtttaat aagcagttac
     4621 gaaagctccc aatatctcta acgttcagct cactccattt cttttcttca aaaaaatcag
     4681 tgattcgtat ctctttctat ttgtcgaaaa agttaacttt aaaaagtaac gccgaaaaaa
     4741 tatgcaaaaa aataaaaaag cagttactat tgctagaata acatcaagga ataataagtt
     4801 ttggttttca gataatagtc gataagttgt gtattggaat tttagataca gtaataaagt
     4861 atttttcatg aaattttgtg agaatcacta ttacaagtat ctttaataaa attaatggtt
     4921 aaggaggaac ttaaagtaga atgtaaatga atagttgtag tcagtcgatt gtgatactag
     4981 actcaaagtt gcaggaataa gtttttcaat ttgtgattgg aagataaaaa atgaaaaaga
     5041 agggcttttt tttatacttt gttgattgta gtgtttattt tccctctgtt tagccaagta
     5101 acattttcta tgactcaacc agtttcaagt gaggaacatg ataacctttt tcgtgctaca
     5161 gaagagttta agaaatatca ggaaaatatt gttccggaga ctccattagc tagtgagcca
     5221 attactgata ttgatggaaa ccaaatagga tggtatatac agtacgaagt tatggtggta
     5281 aataatgact caatagtaaa aaatacagta ggcttagagc taacaccgat tgaaaaggtg
     5341 agttcattgt taacgtatat ttatactgat gaaagtgacg agactgagat cattttaacg
     5401 gattactcaa aaattgctac tgataaaaag tatatattgt ggatgtagac actagtgaaa
     5461 tactttatga tatagaaatt gatgaaaatt tactagatgt agcacaagaa atcgatcatc
     5521 aaaagcagga agctattcga tatgctggta gaaaagggaa aagaaaatat ttattacgtg
     5581 aatgtagcaa aagttagaga agatgaaaat gagtggaaag aattcaagag tcgctactca
     5641 attaactcga ctccgacatt tacagtttac agagaaggat cgatagaaaa aactgttttt
     5701 tggactaaag aaagtggaat gtctttagct gaagtagaag agtttttgga ttatgttagt
     5761 atgcaacagt aagggagttc ttattatgaa aaattttgac acatattatg ttattgtttt
     5821 ttcgatatct ttgctgttaa tattactaac tttaatgcag tataaagagt atccagagta
     5881 ttttatttta acttttgtat atttagtaag tagctacaca cttctatata aattcagaag
     5941 ttcttctaga gtttctaaag tttgtttctt aattagctta ttgattctat aatatttttg
     6001 atttatatag gaaattaaaa atattataga atcaacactc agagaacggg ttggttttta
     6061 atagatttaa taagtactga taatgaaatt tctcctggtt gggaaaacga agttggttac
     6121 cctgtagaag aattttttca agattacttt gcggagtatg ccgataggat aatgagagat
     6181 tacgtaacag ataaaactca agaagtcatc aatcaatccc agtcattaaa tgaattatct
     6241 gataaggcga aagaagaagc agttgccatt atttcttcag ggattagtga ctatattgat
     6301 atgagtggtg aaatcaatta tggcgataaa gacaaaattg tacgcatgat aaaagctttt
     6361 atcaaaactt ttgatgtgaa ggcagatctt aagtttaatg atacttacct agtaggaaaa
     6421 ttaattaata tccttgggac agagaaagat acggaaacgt ttatttcctc tttgtctatg
     6481 ttgtttacag ataagcattt ttcaactcag cttggtggag aggaactagt ggaaatcttt
     6541 taaccattac gcccagcatt aataaaagta tatcaaaaaa gtactcctga tgatttttaa
     6601 gattggtttg agaaataatg tgaaaacact atttttaatg ttaattatag atagagatga
     6661 acgcgtcata tggttaaaag aatgaaaaag tcaaagatac actgctatat ttcactaagg
     6721 atatagcagt gtatctttca tgtcgtttca ctggcagttt tttataaaag gaggtgtgca
     6781 tttcagttgc ttttctttgg tagttagttg aatcgtttaa gaattaacgg atgtggcaga
     6841 caggtgctct gtccaaaaac cagtaaaatc tttgatggtt ccatctttat ttagctgtgc
     6901 ctgttcaaat actgttttgt ttttaccgaa tggatcaatt gcaataattg ttccgatccc
     6961 cgcacgtttg gcagcaagta atcctgagta agcatcttcg ataacaagac aatcagccgg
     7021 agctaagctc agtttgtctg ccgctttaag aaaaatatcc ggttgcggtt tcccagggaa
     7081 agagccgtcg tcatagacaa tcttgtccca gtcaaaccag cgggctaaat cgaaaatatc
     7141 aaaataaaaa gcaacattct ctttgacagt agccgtggca atcgttaatg gaatctgttg
     7201 gtcagtcagc tggtcaagag tcgcttcaag cccttttgtc aactgaagct cgttttcatt
     7261 ttgcaagcaa agcatacgat aatagctttc tttttcttct gacaatacgg cgatctcaga
     7321 ggcggtcaaa tgctctgaaa tgaaatgttt caaaatctcc gtattcgtcc gaccatgaat
     7381 attttttaaa atatcctcgt ctgataaacc atcttttgta tgatcatgta tcatatgcag
     7441 ccaagcagct tcatgcagat gagagtccaa aaacattgtt ccgttgaaat caaatataat
     7501 ccctttcata gttacgctcc tatagtggta ttttcggttg aatcagcaat ttgcagatcg
     7561 acatattgtg catagtgctc ttgaacctcc cgcgagatct ggcgatccgt aatgagcaga
     7621 tcgaaatcat gcaaattata aaacacataa aaatcatctc gattgaattt tttattatca
     7681 acgagtagat atttcttacg ggaattattt agagcgatct gctgtgcttc tccttcttcg
     7741 gtactgtaag tagagatcgc ttcgttgtga acgccatttg aactgataaa tgcttttgta
     7801 aactttagtc ttcttaaatt gtcgttggtg atcggtccga caaatgcgcc ggtatttttc
     7861 cggaaatcac cgccaatcag tagaatttct ggtccatctg cttgagcaag aatatcaaaa
     7921 actggataat tatttgtaat gatccgaatt ttcttgttca gtaaatgcgt cgccaatagt
     7981 tcgattgttg tacctggtcc taagaaaatc gtttctccct cgtgaatcaa ggaggctgct
     8041 gtggcagcaa tttctttttt ttcttgaatc tgcagagtgg ctttttcagt gtgagaaagt
     8101 tcttgatcta aagtgtagga aagactttga gcccctccat ggacacgcag cagcttgccc
     8161 gatttttcta attcatctaa atctctgcga acggtcatat cagaaacatc taattgattg
     8221 atgatttcat taacggtaag gattcctttt ttattgacca tttctacgat agtaagcaat
     8281 ctttcttttt tcaacattgt cttcacctct aatttaagga taatctatca tataaacgga
     8341 caacagtcaa tctaattcga acaaaagtaa acatttaggg tggtttttat gtttgttaca
     8401 atagatttta ttgctgtttt ctggttttta tgattatttg tgtttgtttt tgttgacaaa
     8461 tgttttggat gcgtttacaa tgcaatcagt tagaaagagc gaaaactgat tatagatttg
     8521 aggagagatg tacatgctta aattaccaga agattttatt tttggcggag ccacagcagc
     8581 ctatcaggtt gaaggagcca ctaaggaagg tggcaaaggc gctgttgctt gggatgactt
     8641 tttagaggaa caagggcgat tcagtcccga tccggcaagt gatttttatc atcaatatgc
     8701 aaaggacatt gaattgtgtg aacgttttgg tgtaaatggt ctaagattat ccattgcttg
     8761 gagccgcatt tttcctgatg gagctgggaa accaaatcca gaaggaatcg ctttttatca
     8821 tcgtgtgttc gaagaatgta agaaaagaaa cgtaacgccg tttgtgacgt tgcatcattt
     8881 tgatacccct aaacgtttgt ttgaccatgg agattttttg aatcgagaaa caatagaagc
     8941 atttgtttcc tatgccattt tttgttttca tgaattcaaa gaagtcaagg tgtggagtac
     9001 ctttaatgaa atctatccag ttgcaaccaa tcaatatttg ttaggtgttt tcccaccagg
     9061 aatcaaatat gattttacaa agatcgtcgc ctgtcttcat aatatgatgg ttgctcatgc
     9121 ccgtgtagtg aactatttca aagaaaacga acttccgggt gaaattggtg tagttcactc
     9181 attagagacg aaatatgcgg caacagacgc gccggaagat aagcatgctg cctttttaga
     9241 cgatgcattg tcgatccgtt tcttattaga tgctacctat ttaggctatt attcaactga
     9301 aaccttgact gctcttgatg agatatgtga agcaaatcag gccagctatc attttcctga
     9361 agaagatttc gttgaattga aaaaagcatc cacgcgtaat gactacttag ggatcaatca
     9421 ctaccaatgc cattttgtca aagcttacga tggagaaaat gcaatccatc ataacggtac
     9481 gggtgaaaaa gggacctctg tttataaagt gaagggaatc ggagaacgta tttataaaga
     9541 aggaattcct cgaactgatt gggactggct gatttatcca gaaggtctat atgacttgtt
     9601 gctgcgaatt aaaagcgatt acccacatta caacaaaatc tatatcactg agaacgggat
     9661 ggggtataaa gatcagtttg aagatggcat tattatggat cagccgagaa tcgactattt
     9721 gagagtctat ttggaatcct taagcaaggc gataacagcc ggggtaaatg tgaaggggta
     9781 ttttttgtgg tcgcttatgg atcttttctc atggacaaat gggtataaca aaaggtatgg
     9841 cttgttttat gttgattttg aaacccagaa gcgttaccca aaagaatcag cttactggta
     9901 caagttggtc agtgaaacca aaacgattat ttaataagtg ataatggagg aagaaaagat
     9961 gaataaagag gaattacaaa tgcttggttt tgagatcgtt gcatattcag gcgacgctcg
    10021 cagtacatta ttgaaattat tgaaagaagt aagacaagga aactttgaac atgttgattc
    10081 ggcgttgaaa gaagccgacg aaaatttaac ccttgcacat aattcgcaaa caaaaatttt
    10141 agcggaagaa gcatctggga aagagatgga gatgggattc atttttatcc atgggcagga
    10201 tcatttaatg accactttat tgctgagaga tctgattcaa gactttattg tactgtacag
    10261 acagaatcaa ggataggaga agaacgctat ggatggaatc attaaacaaa tcgaaaaagg
    10321 gaaaccattt tttgaaaaaa tctcacgtaa tagttattta ggagccgttc gtgacggatt
    10381 tttaacagcg atgccagcta ttttattttc aagcattttc attttgattg ctgcggttcc
    10441 tgagattttt ggttaccaat ggcctgaatc tgtctccaca tggctatgga aagtctataa
    10501 ttattcgatg ggtgttgtag ggttactcgt ttcagcaacc actgctcgct gcttggctga
    10561 ttcgatgaat cgtcgcttac cgaaaaacaa agtaatcaac accacctctg tgatgctggc
    10621 ctctgtggta agtttcctgt tgttaagcgt ctctccgatt gaaggtggat tttcaacgga
    10681 atatatgggg acaaagggac tactggcttc ctttgtcgct gcatttttaa cagttaattt
    10741 atataagttt tgtgtgctga aagatattac cattaaaatg cctaaagaag ttcctggaac
    10801 gatttcgcaa accttccgag atatttttcc tttctctttt tcagtatttg cggccgtttt
    10861 aattgattta ggcattcgac aaatcttcca aatctcattt gcagaagcaa tcattacggt
    10921 gctgcagccg ctatttacag cagctgacgg gtacttagga atcgctatta tctggggagc
    10981 gatggcgctg ttttggtttg tcggtgttca tggaccgtcg atcgtggagc cggcaattgc
    11041 ggcaattatc tatgccaatg tggaaaccaa tctgcaattg ttcaattccg gtgaacaagc
    11101 ttctaaagta ctgacggtgg gtttagggaa ttttgtagga actatgggag gaactggggc
    11161 aacattggtt gttcctttta tcttcctact ctttgctaag tctaagcagt tgaaggcagt
    11221 tgggaaagct tcctttattc cagtaagttt cgctgtcaac gaaccattgc tatttgcggc
    11281 acccatgatt ttgaaccctt atttttttat tccatttttg ttaacacctg ttgtaaacgt
    11341 ttgcctgttc aagttttttg tcgatgtatt gggcatgaac agctttatgt acgttttgcc
    11401 ttgggcaact ccagcaccga tcggtttggt tctaggaacg ggcatgggta tcttagcttt
    11461 tgttctagca gcggttctaa tcgtcgttga cttcctgatc tatctaccat tttgcaaagc
    11521 atacgatgct gtcttagtag agaaagaagc acaaaaagca gaggtgatcg caaccgagga
    11581 aggtgcttct gccgatgtac gtgaggaagt acctgtggca gcgagtcaag ttatcagcga
    11641 tgcaaataaa gaaatcaatg tgttggtact ttgtgctggc gcagggacaa gtgcgatgtt
    11701 agctaatgct ttaaaaaaag gtgcatctga atcgaaagca ccgatcagtg caatggcggg
    11761 tgcttatggt tctcattacg aaatgctgca ggattttgat atggtggtct tagcaccgca
    11821 agtcaattct tactatgatg atatcaaaga agacaccgat cgcttaggaa tcaagctggt
    11881 tgcgacaaaa ggagcagaat atatcggatt aacgagagat cctgaaaaag ctgttcagtt
    11941 tattctgaac caatttcaat aaaggaggag ccataatgaa aatgggaatt tggttgcgta
    12001 aagctgtttt atttgcagct gtgttagggg ctttgggggc tgtttcaaaa actgttgaag
    12061 catctgccag cgaatttatt cgcggagcag atatttcgat cctgtcagat atggaaaaat
    12121 ctggtgcaaa atattatgaa gacgatattt caaaagatgc cttagagatt ttaaaaaata
    12181 atggtgtcaa ctatgttcga ctgcgtttgt ggcaggatcc ttataatagt aatggagaaa
    12241 gttatggagc agggacaaat gatctggaaa cgacgattga ccttgcaaaa cgagcaaaaa
    12301 atctgggact taaggtgctg ctggattttc actacagtga tttttgggtt gatcctggga
    12361 aacagaatct tcctaaagca tggcaaggtc tgacctttga agaaatgaat acggcattgt
    12421 acgactatac aaagaacgtt ctgtcggaaa tgaagcagct ggacgtttat cctgatatgg
    12481 ttcagatcgg caatgaactg aatagcggaa tgctctggcc ttatggcaag tcttggggag
    12541 aaggcggggg agaatttgat cgattggctg cttttttgaa gtctggtatc caagctgttc
    12601 gagacactca acctaaaaca actccagtaa tgctccactt agctgatggt ggcgatacag
    12661 gagcatttac ttggtggttt gatgaaatca cgagtcgagg tgtgtcattt gatttaattg
    12721 gagtgtcgta ttatccgtat tggcacggtt caatgaatga acttcaaacc aatatgaatg
    12781 cgatcagcca acggtatggt aaagaagtga tcgtagttga aacagcttat gggcatacaa
    12841 cagcgaatgc cgatactatg ccaaatgctt ttggtgaagc cgaagcagct gcggggggat
    12901 atgaaccaac accgacagga caagcagagt atttgctgga tcttgccgat cggattcagg
    12961 cagtaccgaa taatcgcggg gcgggcttct tctattggga acctctttgg tacaacggaa
    13021 atgtgagttg ggcaacacaa gcaggtatga attatctcag tgttcaatcc actatgggaa
    13081 acgaatggga caaccaagcg atgtttgatt ttgctggaaa cgccttgcca agcttgcgtg
    13141 cgtttaaaca agcaggggcg caaataaatc tagttaaaaa tgcaagtttc gaaagcgatg
    13201 gaccaacgac cagtccttcc aattggcaaa cctggtttca acaaggaacc gctaagggta
    13261 cgatcaaaac ggaagcgggc actttttttg gggactacaa actaactttt tggcaggatc
    13321 aagcatacga ggcatcagct tatcaaaatg ttagtggcat aagcaaagga acttacacat
    13381 tgagtgccta tgtaatgtct gggggagagt ttgatactaa tcagttatat atcaaaaatt
    13441 atggtggagc ggaactaaat actgcggttg ttaaaacaag tgaatggaca aaaattacaa
    13501 tcaaagacat tccaattaca acaggtgttt gtgaaattgg ggtttatagc aaggcttcag
    13561 gaaatgcttg gacgaacgtt gattttgttt cattggtgaa acaatagact gcggatcgtt
    13621 tcgattcaag atcggaggtt cccttcagcc acatcgttag cgtagaaggc aggaatcgaa
    13681 cctgcacctc gcctttgcca aaaataacca gaaacgattt ccagtcattt ttaccaaaac
    13741 gagtaaagca ccagacttca tctacacttt gtattatgct aaagagagtt caacgatttc
    13801 gtaagaagca atgaaaaaat tgcaaaaagt tgtgcagata acataaagtt tgtttaagaa
    13861 agggtaacct tagaattccg tcgtgaatat gatttgagca cttagatgga tagaaaaaag
    13921 ttctcggaaa acagttcgtt tttcgagaac ttctttttat gataagaatg aaacaaatta
    13981 acttatatta tagtctcttt tggtagggaa ccatcttatt tttaatgaaa atcaagcatt
    14041 ttagctattg aagaatgttg atttataggg attccccctc catattatgt tggctcttta
    14101 ttcccactcc actgttgctg gtggtttaga agtgatgtca tacacgatac ggttgacgtg
    14161 ggctacttcg tttacgatac gtacagagat tttttgcaag acttcccatg ggatacgggc
    14221 aaagtcagcg gtcattccgt cgattgaagt tacagcacgg ataccgactg tgtagtcgta
    14281 agtacgtccg tcacccataa caccgacaga gcggatccct ggaagaacag tgaaatattg
    14341 ccagatatca cggtcaagtc cagcagctgc gatttcttca cgcaagatag catcagattc
    14401 gcgtacgatt tctagcttgt cttcagtgat ttcgccaaga acacgaatac caagaccagg
    14461 accagggaat ggctgacgcc aaacgattga atcaggcatg cctaactgag tccctaattc
    14521 acggacttcg tctttgaata atgtgtttaa tggttcgatc aattcaaatt gcatatcttc
    14581 tggcagtcca cctacattat ggtgagattt gatcgtctgt gctgtttctg taccagattc
    14641 gataacgtca gtatacaacg ttccttgtgc taggaaagag acgccttctt caccagcaag
    14701 ttttgttgct tcgtcatcaa aaacatatac gaattcattc ccaatgattt tacgtttttg
    14761 ttctgggtca gatacacctt tcaacttgtc taagaagcga tctcttgcat tgactttgat
    14821 gatgttcaag ccgaattttc cgcctagact ttccattact tgttccgctt cgcctttgcg
    14881 tagcagaccg tggtcaacaa agatagatgt caattggtca ccgatcgctt tttgtaaaag
    14941 aaccccaacg acactagaat caacgccacc tgaaagacca agcaagactt ttttgtcacc
    15001 aacttgttca cggatttttt ctacttgcat atcgataaag ttatccattg tccagttacc
    15061 ttcacagtga caaacatcaa aggcaaagtg gcgcagcaat tcgattccgt attcggaatg
    15121 acgtacttct gcatggaatt ggataccata aaaattacgt gttgtgtctt ggatagaagc
    15181 aatcggacaa tctttgcttg ttccgactgt ttcaaagcct tcaggaactt tagttaccaa
    15241 gtcaccatgg ctcatccata ctgtttgttg ttttggtgtt tctttaaaca agacagcttg
    15301 atcgcttgtt acttctaaca aggctttccc atattcgcgg tttttagcag gctctacgga
    15361 accgccaagt ttgtacgtga tcaattgcat accatagcaa atccctaata caggaatacc
    15421 taaattgaag atctcaggat cgatcccaaa tgcattctca tcatatacac tgtttggtcc
    15481 gccagaaaga atgattccct ttggattcat cgcttttact tcttctgcag taatgcggtg
    15541 gctcaataat tcggaaaaga ctccaagatc acgaatacgt cttgtgatca attggttata
    15601 ctgactaccg tagtctaata cgataatctt ttcaacgttc gtcatatcgg caacgtttgt
    15661 caaatttatt acccctatcc ctttaaattt tatctcaaaa tagtcaaact attttaaaat
    15721 gctaatattt tcgcatgaaa atcgaatcga tgatatgatt ttcagtttta tggagaatta
    15781 tatcggctct tcctcgtgta ggcaagatat attcctgtaa atttttcaaa ttcacatttt
    15841 tccatacatc ttttgccatt ttaaaggctt cttcacgatc tccgatagca aaacgataat
    15901 aataattctc tggattttgg aaagccgtat ccagtaacgc accaaaacga tccaaatacc
    15961 atttttcgat cagcacggga tcggcatcga catagacaga aaaatcaaaa aagtcgctga
    16021 cgtagatttg ctgattcgca ggtaattgaa gcgtattgat cccctcaacg atcaagatat
    16081 caggctgctg gatcaattcg tacttgtctg gaatgatatc atacacatca tgtgagtaaa
    16141 caggtacttt tatctcttcc tgaccagatt ttacacggtt caagaaatcg atcagcatct
    16201 ccatatcata actttcagga aatcctttac ggtccatgat tccacgctct tttaatatac
    16261 gatttggata aagaaatccg tcagtcgtga tcagctgaac gtttcttcgt ttgaaggtac
    16321 gagacaaaat cgtctgcagc agtctggctg ttgtactttt tccaacggct acacttccgg
    16381 cgatcccgat aataaaaggc ggtacttcta cgtatttatg aagaaataaa cctttactca
    16441 agaacaaaga ttcaaattct ttcatataaa gataaatcag atgagcaaga ggtacataga
    16501 tatctcgaac atcctgcatg gaaatgcggt cattcaaact tttgatatta tctagttcat
    16561 cttgtgtcaa aggtgcttgc ccattacgat aaaattctcg ccattcttct cttgccacac
    16621 ggtagtagtt cattggatca ttcatattag cctcctgcgt tctcccatca atggaactat
    16681 tttaacacaa aataaacgca aaccccacca cttaagagag attgccgatc tttttgtata
    16741 atctttataa tagaaagaaa cggagtctta ttcttgactt gcgcacggat acataaaagg
    16801 atagactgtt gtcatgaaag aaatgagaag gatggaagac atgacaaatc attactattc
    16861 tgaacgccca gatacggcgc acgattttga acaatgggct tttgagttaa aaggcagaac
    16921 ctttcaattt gtaacagatt ctggtgtatt ttcacgtgaa acagtggatt atggttcacg
    16981 tgtcctgatt gatgctttcg attgggaaga gttacctgat gaaggaaaga tcttagatgt
    17041 gggatgcggg tatggaccga ttgggttagc tttggcattt gcgacacagc ggtttgttga
    17101 aatggtcgac attaatgaac gagcggtaag tctagcgcaa ggaaacgcaa agcgtaacca
    17161 aatcgaacat gttgatatcc atcaatcgaa tatctatgaa gcagttcatg aagaaaccta
    17221 tgcagctatc gtaagcaatc cgccgatccg tgctggaaaa aaagttgtac atgaaatttt
    17281 gacaggcgca tatcctcggt tgaagaaggg cggtacactg acaattgtga tccagaagaa
    17341 acaaggagcg ccgagtgcgc aaaagaaaat ggaagaaaca tttggtaatg cagaaatcgt
    17401 cacaaaagat aaaggatatt atatcatcaa aagtgtgaaa gaataactag ctgaaaaata
    17461 gtggagcgac gaaagagtgg aactgggcgt gcatgtacgg gtacgtatca gttctttttt
    17521 tgttgagaag tttttaaaaa caaagaacta ttttttcttc aaaaagaaat agtaggaaca
    17581 gagtcgttgt tttttacgat aaaataaata agatacagaa cttgtataga agactgccaa
    17641 gtcggtagat aaaggaggtt tttatcaaaa aacatacttt ccttttttct ctattctttt
    17701 cttgggggtt ttggtacact gatagggaat ttagatagaa gggagccaat aggatgaagc
    17761 tgaccatcag agatatcgca gaaatggcag gcgtatcagt aacaactgtc tcacagattt
    17821 tgaataacaa aggaagcaga tttagtgata agacaagaaa aaaagtgttg tcgatcgtga
    17881 atgaacatca ttataagccc gattatttcg cttcaaatgt gatcaatcga cattcaaaga
    17941 ccatcggaat gatcgtccct gatgtcactg atttcttctt ctccaaactg atcgaaggtg
    18001 tagagagcta tttgaatcct ttgggctatg tgatcatgtt atgcaattct cgtcatagcc
    18061 aagaaaatga gatcaagtat ttacaagagc tttcccaccg ctcagtggac ggaatcttgc
    18121 tggccacacc aaatatttta cctgagaaat atgcattaga cagcggtttt tatcaaaaaa
    18181 tgccaatcat tttaatcgat cgcggattga atcgtcggga taacggacgg cttatcgtaa
    18241 aggaatatga gggcgcctat caagctgttt cgctgtttat tgaaaatggc catacgaaga
    18301 tcggcatgct gaaggaaacc actggatact atcaattaga agaacgtttc aacggatatc
    18361 gtcatgcgct gaaagatcat ggcctgcctt ttaatgggaa atttgttgaa catggagagt
    18421 tgactgtcca aggtggatat actgcatcaa aaaaattact caaacataaa gatatcacgg
    18481 ctattttttg cgggaatgat gccatggcga tcggatgcta tcaggcgatc gatgaattgg
    18541 gcaaaaaaat cccagaagat atctcagtca taggattcga tggattaaag ctgtcagaat
    18601 acatgattcc gcaattgacg acagtgcagc agccaagttt tgatattgga ttttatgctg
    18661 cacgtttcct gatcgatacg atcgaatttc ctcaaagaaa agttccgaac aaagtgttcg
    18721 aaacaaaatt aattattcgt gagagcgtaa aagtattgac aaagggctaa ctcggcggta
    18781 tattgcttat gtaagcattt acaaaggcta gttagtgctt tttttatttt tagtaaacca
    18841 gtttactaaa gtggtttacc tttcctaaaa ataatgtata ataccgaatg aagacaagga
    18901 ggatcaggca atgcgaatgg tggatctgat tgaaaaaaag agggatggaa acgagctgtc
    18961 gaaagaggaa atcgagtaca tcgtcacgaa ctatacgaat ggaaagattc cagattatca
    19021 agtcagtgca ttattgatgg caatttttta tcaagacatg acaaatgaag aaatcacgaa
    19081 cttaacattg gcgattgcta attctggtga tgtcatcgat ttgagttctc ttgaaggaat
    19141 aaaggtagat aagcattcga caggtggcgt tggtgacacg accacattga ttttagcccc
    19201 cctagtagct agtgtcggag tgacagtggc taaaatgtct ggacgcggtc taggttacac
    19261 aggtggtaca ctagacaagc tagaagcaat tcccggattt cagatcgagc tttcggatga
    19321 agaatttgta cgcattgtta atgaaagtaa agtagcggta attggtcaat caggaaactt
    19381 ggcaccggcg gacaaaaaat tgtatgcctt gcgtgacgtc acagcgaccg ttgattctct
    19441 tccgctgatt gccagttcga ttatgagtaa aaaaatcgcg gcaggagcag atgcaatcgt
    19501 tttggatgtg accactggag acggtgcttt catgaagaac atcgaagatg caagacgtct
    19561 ggcaaagacc atgacgagca ttgggaaact ggctaaccga gaaacagtgg cggttatctc
    19621 agatatgtca gaacctcttg gcgaagctat cggaaatagt ctagaagttg tcgaggcaat
    19681 cgaaacatta caaggaaatg gtccagaaga tctagtagaa atgtgctacg ccttaggaag
    19741 ccaaatggtt gttcttgctg gcaaagctaa gacaaccgat gaagcaagaa cgttgcttca
    19801 agaagctctg gaatcaggta aagcgttagc aaaattcaaa gaaatgatcc aaaatcaagg
    19861 gggagaccca gcgatcgtgg aacatccaga acgaattttg actgcacgct acacaatgga
    19921 gcttcctgct aaacaaagcg gagttgtgtc aaaaatcgtg gcaaatgaat taggaattgc
    19981 tgcaatgatg ctaggtgctg ggcgtaaaac caaagaagaa gatattgatc acgctgttgg
    20041 attgaaactg cataaaaaaa ttggtgacac tgtgacaaaa ggtgagtcac tattgacgat
    20101 ctatagtaac gacgaagaaa tcacttctgt cattgaatta ttatataaaa atattgaaat
    20161 tggtgaatcg gcaatgaagc caacgttgat tcatgacatc atcacagaat aaaggaaaaa
    20221 agcggacttt accgttttaa agaaaagggg aaaagaaatg gaactaaacc gaatgatcga
    20281 ccatactatt ttgaaagcag acgcatctaa agaagctgtg atgcaaatta tcgaagaagc
    20341 aaaaaaatac catttttatt ctgtctgcat caatccagca tgggttgcgc tagcgaaaga
    20401 acaattacaa ggtgagccag tagccgtatg tacagtaatc gggtttcctc taggagcaaa
    20461 cacacccgag acaaaagcat ttgaaacaac agatgccatc aataacggtg cagatgaagt
    20521 cgatatggtc atcaatatcg gtgctttgaa atcaaaggat gatcaacaag tccaaaaaga
    20581 tatcgaagca gttgttgaag cagcaaaaga tcgtgcgctt gtcaaagtta ttattgaaac
    20641 atcgttatta gatagagaag aaatcatccg agcttgcgaa atagcaaaag ctgcaggcgc
    20701 tgattttgtc aaaacatcaa ctggtttctc aacaggcgga gcaaaggtag aggatgtccg
    20761 tttgatgcgt gaaactgtag gaccagaaat gggtgtcaaa gcttccggcg gtatccataa
    20821 cgaagaagaa gcaatggcaa tgatcgaagc tggtgctaca agaatcggaa caagcgcagg
    20881 agtagcaatc gtatctggtt caactggtga aggctattaa ttttttcaaa ggagaagctc
    20941 atggcagaac tcaaacaaga atggatcaac acagctattg aagcgttaga taaagcctat
    21001 gttccttatt cccattttcc ggtaggcgct tgtctcgtaa cggaatcagg caagatttat
    21061 caaggcatca acattgagaa tgcatcattt ggattaacga attgtgccga gcgcacagct
    21121 tttttcaaag cagtatctga aggagaaaaa acatttacgc atctagtcgt agcggggcac
    21181 acccccgatc ctatctctcc ttgtggtgca tgtcgacaag taatggcaga attctgttcc
    21241 cctgatatgc cggttacgct agtaggagac aatggtgtga cgaaggcaac gaccgttcgt
    21301 gcactgctgc cttatgcatt tactgaaaaa gatctataac tcatgttcat tcagtcagct
    21361 gactgttgaa aaataaaaca attattttag gaggctttac agaaatgaaa aaagcaaaat
    21421 tatttggttt aggtgctgtc gcactagcag cagggttatt tttaggtgca tgcggcaaca
    21481 acggctctac tgattcatcc ggtggcaaaa gctcatcaga cacaacaact gcagcattga
    21541 tcacagacac aggtggtgta gacgaccgtt cattcaacca gtcagcttgg gaaggactag
    21601 aaaaatgggg gaaagatcaa ggactttcac gaggaaatga tggcttccaa tatttccaat
    21661 cttcaaatga atcagattac atcccaaata ttgaccaagc attgaatgct ggattcaaaa
    21721 cgatttttgg tattggttat aaactaaaac cagcgatcca agaacaagca acaaacaata
    21781 caggtacgaa ctttgttatt atcgatgatg tcatcgatgg attagataac gttgtatctg
    21841 caactttcaa agacaacgaa gcttcttatc ttgcaggtgt tgcagcagct tatacgactg
    21901 aaacaaacgt agtcggcttt atcggcggtg taaaaggtga agtaatcgat cgttttgatg
    21961 ctggattcaa agccggtgtt gatgcaggtg cgaaagaatt aggaaaagaa atcaaagtat
    22021 tgaatcaata tgctggtgac ttctctgctc cagataaagg acgttcaatt gctcaaggta
    22081 tgtatgcaca aaacgcagat atcatcttcc atgcttcagg cggtacaggt aacggtgtat
    22141 tccaagaagc aaaatcattg aacgaatcag gcgacaaaaa agtttgggtt atcggtgttg
    22201 accgcgacca atcagacgaa ggcgaataca cattaaacgg cgaaaagaaa aacttcacat
    22261 tgacatctac actgaaagca gttggaacag tagtagaaga tttggcacaa aaatcagcgg
    22321 acggtaaatt ccctggcggc gaacacactg tttatggatt gaaagaagac ggtgttggtc
    22381 taacagaagg acaactttct gacgaagcga aaaaagcagt tgatgaagca aaagaaaaaa
    22441 tcatttcagg tgacgtaaaa gttccagaaa ctcctgaaga aaactaaaac catttctata
    22501 gccttgttag acacatgttt gacaaggcta tggtttgatt agaacaagaa ttgttcattt
    22561 atccaagctg ccttgtgaca gtttggataa gggaacaact ccctgaagta atgtttgttt
    22621 caaaagaaga acgctcacta agcgtgaatc ttagtcaatc gttgccctag atagaaatcg
    22681 ttaggatgtg aaaaaaaggt ggttcaagaa aatattgtca tagaaatgca gaatattacc
    22741 aaagaatttg gaacattcaa ggcaaatgac aacatcaatt tacaagtaaa ggcaggagaa
    22801 attcatgcgt tgcttggaga aaacggtgct ggcaaatcta cattgatgaa cgtgctttcc
    22861 ggattattag agccgacatc agggaaaatt ttgatgcgtg ggaaagaagt aaagatcaca
    22921 agtccgacca aagccaacca attagggatt gggatggtcc atcagcactt tatgcttgtt
    22981 gatgccttta ctgtaacaga aaacatcgtg ttgggaagcg agcctagtcg cgcagggatg
    23041 cttgaccata aaaaagcacg aaaagagatc caaaaagttt ctgaacagta tggattgtca
    23101 gtcaacccag atgcttacgt tcgtgatatt tcagttggaa tggaacaacg ggtagaaatt
    23161 ttaaaaacac tttaccgagg agcagatgta ctgatttttg atgagccgac agctgtattg
    23221 acccctcaag aaattgatga attaatcgtg atcatgaagg aattagtcaa agaaggcaag
    23281 tcgatcattt tgattacgca taagttagat gaaatcaaag cagtagctga ccgctgtaca
    23341 gttatccgcc gtggaaaagg aatcggtaca gtcaacgtta aagacgtaac ctcacaacaa
    23401 ttagccgata tgatggttgg aagaacggtt tcattcaaaa cgatgaaaaa agaagcgaag
    23461 cctcaagaag tcgttttgtc tattgaaaat ctagtggtga aagaaaatcg tggattagaa
    23521 gccgtgaaaa acttgaactt agaggttcgt gctggcgaag tacttggtat cgctggaatc
    23581 gatggaaacg ggcagtcgga gttgatccaa gctttgactg gtttgcgaaa ggcagaaagc
    23641 ggacatatca agctaaaagg ggaagacatc actaataaaa aaccccgaaa aatcactgaa
    23701 catggtgtag gacacgtgcc agaagaccgt cataaatacg ggttagtcct agatatgaca
    23761 ttatctgaaa atatcgcact acaaacctat catcaaaaac cttacagtaa aaacggcatg
    23821 ctgaattatt cagtaatgaa tgaacatgcc agagaattga tcgaagaata tgatgttcga
    23881 acaacgaatg aacttgttcc cgcaaaagct ttatcaggcg gaaatcagca aaaagcaatt
    23941 atcgctcgtg aagtcgaccg agatcctgat ctgttgattg ttgcaaatcc aactcgtgga
    24001 ctggatgtag gtgcgatcga atttattcat aaacgtctga tcgaacaaag ggacaaatac
    24061 aaagcagtgt tactgatcag tttcgaatta gaagaaattt taaatgtttc ggatcgtatt
    24121 gctgttatcc atgaaggaga aattgtcggt atcgttgatc cgaaagaaac atctgaaaat
    24181 gaattaggtc tattgatggc gggctatacc ttagaagaag cacgaaaaga gttagaaaag
    24241 ggggctggcg cacatgaatg atcgcagcga aaaattccgt aatttattag tgcctgtact
    24301 ttcagttgtt ttaggattga tcttaggcgc gatattgatg gctgcttttg gttttcagcc
    24361 catccaaggt tattcttcta tgctagatgc agcccttggt tcgcaaagag ggataggtga
    24421 aacgttaagg caagcaacgc cgttgatttt tacagcatta ggtttttctg tagctaactc
    24481 agctgggttt ttcaatattg ggctttctgg acaagcactt tgcgggtgga tcgtcagtgt
    24541 ctgggtggca cttgcgtttc cagatctgcc aaaagttgtt ttgcttccgt tatgcgtgat
    24601 cttaggtgcc ttagctggtg cgatggcagc ggctgttcca ggtttacttc gagcattttt
    24661 tggaacaagt gaagtgatcg taacaatcat gatgaactat atcttattat acgtaagcac
    24721 atttgcttta catgaagtga tgcctgaaag ctatcgctca agtttggact cttccaatat
    24781 gatcagtcaa aatgcctcac tgcggttacc atggttaaca caagcttttg gcggctctcg
    24841 cgtaaactca ggattatttt tagcaatcat cgcgcttatt ttcgtttggc tgctgatgaa
    24901 gaaaacgact ttaggatttg aaatccgttc tgttggattg aatccttttg cttctgagta
    24961 tgcaggaatg agtagtaaac gtacgatcgt tttgtcgatg gtcatttctg gcgctttggc
    25021 agggcttggc ggtgtggttg aaggattagg aacgtatcaa aacttctttg ttcaaacgac
    25081 ttcgctatct attggatttg acggaatggc tgtttccttg cttggttctg gatcagcaat
    25141 cggtattctt ttatcagcga tcttgttcag tattttgaag atcggtggac ttggcatgca
    25201 gacaggagca ggtgttccgt ttgagatcgt caatgtatcg attgctttga ttatcttctt
    25261 tgtcgggatc aacttcctga ttcgtttcgt catggctaaa gtcttccaag ggaaaaaaca
    25321 agaagagatc gttgcgacaa tagaatcgaa accaacgaat caaaaccatg aagggggcga
    25381 actataatgt cgactatcga attaattgcc ttgatcctta cttctacact ggtttattca
    25441 acaccgctaa ttctaacgtc actaggcgga actttttctg aacgaagcgg gatcgtaaac
    25501 gtagggctag agggaatcat ggtcatgggt gcttttagtt ccatcgtttt caacttgacg
    25561 atgagtccat cacttggttc tatgacacca tggatcgcta ttttagtggg tggcgtcgta
    25621 ggtatcctat tttctctgat tcacgcagta gcaacagtca gtttgcgtgc tgaccatatt
    25681 atcagcggga cagtaattaa tctgatggca cctgctttag gtgttttctt gatcaaagca
    25741 tggtatggta aaggtcaaac tgataacatc acgcagaatc tggggtattt ttctttccca
    25801 ggattagcag atattccggt cattggacca attttcttta aaaatacatt cttacctgct
    25861 tattgtgcta ttttgattgc tgtcctagct tgggtgatca tcttcaagac gaaattcggc
    25921 ttgcgcctcc gttcagttgg tgaaaatcct caagctgccg acacattagg tatcaatgta
    25981 tatgctatgc gttattcagg cgtactgatt tctggcttgt taggcggtat gggcggtgct
    26041 gtttttgctc agtcgatttc cggtaacttt tcagctggta cgatcgttgg acaagggttc
    26101 atctccatgg ctgctatgat ctttggtaag tggaatcctt taggggcaat gggtgcctca
    26161 ttgttctttg gttttgctca aagcttgagt atcattggcg cacaattacc agttatttct
    26221 tcgattccgc cagtattgct gcaaattgct ccatatttgc tgacaatcat tgtgctggtt
    26281 gttttcttag ggaaagcttc tggtccaaaa gcaaacggga agaactatat caaatcaaaa
    26341 taatctttta gcgaataaaa agggtgggtt acgggaagtg tttttcgtct cgcccttttt
    26401 gttttaaagg caataaaaat aaaaagaaag aaggaataat catgtttaaa cgcgtacatc
    26461 tgatcgtcat ggattcagta ggaattggag aagcaccaga tgccgataag ttcggtgatg
    26521 ttggttctga tacattagga cacattgcga aagaagcagg attgaacgtt ccacatttag
    26581 agcagcttgg tttaggaacg atccgtcctc ttgaaggtgt ggaatcagtg aaagatcatc
    26641 aaggctatgc gacgaaatta gaagaagttt ctgtagggaa agatacgatg acaggccact
    26701 gggaaatcat gggattgaat atcaaaactc catttcgtgt gtttcctaat ggattccctg
    26761 atgatctgat caaacaaatc gaagaatttt ctggccgtaa agtcgtatgt aacaaacctt
    26821 atagcggaac agctgttatt gacgattacg gtgaacacca aatgaaaaca ggcgatttaa
    26881 ttgtatatac ttctgccgat cctgtccttc agatcgcggc acatgaagaa atcattcctt
    26941 tagaagaatt atatcggatt tgccaattcg ttcgtgatat aacaaaagat gatccgtata
    27001 tgattggccg aatcattgca cgtccgtacg tgggtgaacc aggagacttc acacgaacaa
    27061 gcaatcgcca tgattatgcg ttgaatccat tcggacacac agtccttgat tctttgaaag
    27121 aagcaggaaa ggacgtgatt gctgttggaa agatcaacga tattttcaac ggacaaggaa
    27181 tcactgaatc tgtccgcacg aaaagcaata tggatggcgt tgaccagctg ctgaaagtta
    27241 tgaaaaacga tttttcagga atcagcttta ccaatttagt agattttgat gcattatacg
    27301 gtcatcgtcg ggatgtagtc ggctatgcgc atgcgattga agaatttgac ttacgaattc
    27361 cagaattgct tgaagcgatg gaagaagacg acttgctaat gattacagcc gatcacggaa
    27421 acgatccgac gtttactggt accgatcata cgcgagaata tgtaccgtta ttggtttaca
    27481 gtaaaaaaat gaaagaacaa ggggcattgc ctcaaggata ttattcagat atttcagcaa
    27541 cgattgctga gaactttagt gtttcttcga ctgaaaatgg cgaaagcttc ttgaaattat
    27601 taaaataatc tttttaaata ggagaaagca aatgaccaag ttaagcgaga tgttacaaga
    27661 aacgacccag tttatccttg ataaaggagt aaaagaaatt gattttggtt tgattctagg
    27721 ttcaggatta ggtgaattag cagatgaaat cgaagatgct gtaatgattt cttatgatga
    27781 gatcccgttc tttcctgttt cgacagtagt tgggcatgca ggaaagctag tttatgggtc
    27841 tctggcaggc aagaaagtgt tagccatgca agggagattc cactactatg aaggtcattc
    27901 catgcaaaca gtgacctatc cagtacgtgt gatggccgga ctaggggccc attcagtcat
    27961 tgtgactaat gcatgtggtg gtgtcaacga atcatttgta ccaggagatt tgatgctgat
    28021 taccgatcat atcaatttta ctggtgaaaa tcctttgatc ggtccgaatg aagatgcaat
    28081 gggccctaga tttccagata tgagtcaagc ttatacgcct gactatcaag caacagcaaa
    28141 agcagcagca gaaaaactag gtttggatct taaagaaggc gtatatatgg gctattctgg
    28201 accaacttat gaaacgcctg cggagatccg aatgtctcgg acaataggag ccgatgcagt
    28261 agggatgtcg actgttccag aagtcatcgt agcagctcac agcggattaa aagtgttagg
    28321 aatctcttgt attaccaact tagcagcagg tatgcaagcg aatctgagcc atgaagaagt
    28381 tgtagaaaca actcagagag tcaaacaatc ttttaaagca ttggttaaag aaacattgac
    28441 attattgtaa taggaggaac aataaaatga gtgtgcatat tgaagcgaaa gaaggcgaga
    28501 tcgccgataa gattttactt ccgggagatc ctttgcgagc taaatatatt gcggagactt
    28561 ttttggaaga tccagtttgt tacaatcagg tacgcggtat gttaggcttt accggcaaat
    28621 ataaaggaga acgtatttct gttcaaggaa caggaatggg aatgccatca gccaccatct
    28681 atgcccatga attgatccag tcgtatggtg taaagaaatt gattcgagta ggtacatgcg
    28741 gtgcattgtc aaaagacgtc catgttcgtg atttagtact agcacaaggt gcagcgacca
    28801 gttcttcgat gatcgaaaaa aatttccaag cctttcattt tcctccaatc agtgatttca
    28861 atctattact gaaagcatat gagatagcta aagaaaaggg ctatacggtt catgtaggaa
    28921 atgttctttc ggaagattct ttctacaaag atgatttgac agagacgttc caattagctg
    28981 aacttggcgt tttaggagtg gaaatggaag cagcggctct ttattactta ggcgcgaaat
    29041 accatgtgca gacattaagt ttgatgaccg ttagcgacca tttgatcacc ggtgaagaaa
    29101 caacagctgc agaacgtcaa tctacgttca atgaaatgat cgaagtagga ttggaaacag
    29161 cgatagcaga ataagaagga aatgaaaaag ctgccctgca ttaagtaata agaacatcca
    29221 agcaaaaata gctcctcatg tttttgactt ggctggactt attattgcaa atgtaagctt
    29281 ttgaacaccg tttaccaaga ggttgtgaat ctgccgttta actctgagtc acaaggaaca
    29341 atttcaaaaa acagcttttc atgtttgatg aatattgtga cttgtgaccg agaagttggc
    29401 aggagaaaac cgtttatacg aaaaaaggga ctgtgacaaa tcgtgtcaca gtcccttttt
    29461 tatagtgttt tttataggta gtatttttta gcaactgtta agtcatcatt taattcataa
    29521 acaagtggtt gacctgttgg aatttcaaga tccatgatgt cttcgtcaga gataccttcg
    29581 atatgtttag ctagggcgcg taatgagtta ccatgtgcag ctactaaaac agttttgttg
    29641 tctagtaatg ctggagcaat ttcatcttgc cagaatggca aagcgcgttc taatgtcact
    29701 ttcaagtttt ctccaccagg aatatcgcgt ttgtctaaca ttgcgtaacg acggtcattt
    29761 gctgcagaac cttcgtcagt ttcttccatt aatggaggaa gagtatcgta agaacgacgc
    29821 cagatgtgca cttgttcgtc gccgtatttt tcagctgttt cttttttgtt caaaccttgt
    29881 aatttaccat agtgacgttc gtttaagcgc catgatttga tttgtggtac ccaaagttga
    29941 tctgaatatt ctaagatcaa gttacatgtt ttgattgcac gagttaaaac agaagtgtaa
    30001 gcaacatcaa attcgattcc agcttctttg attttgcgtc cgccttcttt tgcttcttcg
    30061 attccttctg gtgctagatt tacatcagcc caaccagtaa attggttcaa tgcattccat
    30121 tcactaagac cgtgacgaga aaaaactaat tttggcatgt ttagctctcc ttttaagata
    30181 ggtttatatt tctgaaattt tcagttctgt tttattgtac aattttttgg aagagatttc
    30241 catcgaaaaa ttagataaga ttctatgaga aaacaagtag agaatataaa aaaacgaaca
    30301 gtgcccacgc aactgttcgt ttttaggtat tagatgcata attgctcgat ttctttttca
    30361 ggtaaattga tatatttata acatgttcca actgaaacac cgcattttga agaaataaat
    30421 tggatcgttt cttttttttc ataatagaga cgtcggattt ttttgatcgt ttttgcatcg
    30481 atttttggtc ttccgccgat ttttcctttt ttacgagctt catctaatcc aactaatgta
    30541 cgttctttga tcaatgcgca ctccatgttt gctaggtttt ccattaattg gaaatagatt
    30601 tttcccattt cgcgcgtatc gattccttca tcaagactaa tcaagtgcag cccgcgatct
    30661 ttgaataatt gcgtgagttc tgttaattgc cgcgtagatt tgcctaatcg atgaagttgg
    30721 caaatgacta gtgcatcgcc ttgttgcatg tcagcgatca ctgactccaa atgaacagca
    30781 gtagaatcat tttgttccac ttcatatgtc tcatggaaaa tttcttgaca accaaagtca
    30841 gataattgat caacctgagt tgttaaatca acgtcattta tggttgtgcg tgcataacca
    30901 ataattttca tggtgattcc tttctagcag tgattttcta actggatata acagtaaaaa
    30961 tgtagcataa agtacgtcaa aaagatagtt ttttcgcaaa ttttccgctg tcttttttta
    31021 ttggatgatc aagcagtttt tctttattta cgacggattg cgtcaagaat ctcaacgcct
    31081 ttttctgagc cgacaactac tttagaaacc atgtttagga aaagcccatg ttctactacc
    31141 ccaacaagtt catctaaata ggtagctaag ctttctggct gttcgatgac ttctagatgc
    31201 agatcaatga tgtaatgacc accatctgtg gttaagactt ctccgttttc gtctgtacgc
    31261 aagacgggtt ggaatctttt ttcttcaaaa atatgaagta gctgttggga gccataaggg
    31321 atcacttcaa ctgggagtgg gaacttccca agtttatcga ctaatttaga agaatcaacg
    31381 atccaaatcg tttcttttga gtacgtagcg actattttct cgaaaagcaa agcagcgccg
    31441 ccgcctttga tcccttgaaa atcagcactg atttcatcag caccgtctat tgtcaaatcg
    31501 actacgggga cttcatcaat actctttaac ggtattccta atttttccgc ttgttctttt
    31561 gtcgcatttg aagtagtaac tcctgtgata cgtaaacctt cttcacgcat acggcgacca
    31621 atctcttcta ccatataata ggcggttgaa ccggtcccga gacctacgat catcccgtcc
    31681 tttacgaatt tcgcggcttc aatgccagct aattgtttta aattcatctt tgcgtccacc
    31741 tttttttagt ttgagcatcg tgctttcctc actattttag cgtgatttac ggtaaaaaga
    31801 taccattctt ttttattcca ataaagcttt ttcaaaaaaa attgcatctc ttgttgacat
    31861 gttttttcat tcgtgatatt ctaacaaagg tgctttatct acaggtttct gtgaagaaac
    31921 gtgctgactg tttatcaaag catatttgat aaggatgcag acattgcatt tattttttat
    31981 cacaaaaaaa gaaccacctg gatgtgtgga actagacgca aatcgtggga aggaggaaac
    32041 aaggaatgcc tacaattaac caattagtac gtaaacctcg taaatcaaag gtggaaaaat
    32101 ctaattcacc tgctttgaac aaaggatata acagctttaa gaaaactcaa actaacgtaa
    32161 actcacctca aaaacgtggg gtagcaactc gtgtgggtac aatgacacct aaaaaaccga
    32221 actcagcttt acgtaaatat gcacgtgttc gtttgtcaaa cttaattgaa gttactgctt
    32281 atatcccagg gattggtcat aacttacaag aacacagtgt tgtattatta cgcggtggtc
    32341 gtgtaaaaga cttaccaggg gtacgttacc atatcgttcg cggtgcttta gatactgctg
    32401 gtgttaacga ccgtaaacaa agccgttcta aatacggaac taaaaaacct aaagcttaat
    32461 tttaattatt aaacaaatga aacttgcttc tactatatag aagtaattac tatgaacaat
    32521 atttcggaag gaggagttac ggatgccacg taaaggtcct gttgcaaaac gtgatgtttt
    32581 accagatcct atttataact caaaattagt aactcgcttg atcaaccgtg taatggttga
    32641 cggaaaacgc gggattgcag ctaacattat ctataatgcc tttgacttga tcaaagaatc
    32701 tacaggtaac gatccattag aagtgtttga acaagcaatg aaaaacgttc aacctgttct
    32761 tgaagttaaa gcacgtcgtg ttgggggttc aaactaccaa gtaccagttg aagttcgccc
    32821 tgaacgccgc gttactctag gtcttcgttg ggtagtaaac tacgctcgct tgcgcggtga
    32881 acacacaatg gaagaacgct tagcaaaaga aatcatggat gctgcaaaca acacaggtgc
    32941 ttctgttaaa aaacgtgaag acactcataa aatggctgat gccaaccgtg cgtttgctca
    33001 ctatcgctgg taagattctc tctgtttggt cggcttttat aagtcgacct cagatagtgt
    33061 ccttattttt agcaactaga tagtaaagag aggagaaaca aggagaatga ccagagaatt
    33121 ttctttacaa aacactcgta atatcggtat catggcccac atcgatgctg gtaaaacgac
    33181 tgctactgaa cgtatcttgt actatactgg taaaatccat aaaattggtg aaactcatga
    33241 aggagcttca caaatggact ggatggaaca agaacaagaa cgtggtatta cgatcacttc
    33301 tgctgctact acagcacaat ggaagggtca ccgaatcaac atcatcgaca caccaggaca
    33361 cgtagacttc acagttgaag ttgaacgttc attgcgtgta cttgatggtg ctgtaactgt
    33421 tcttgatgca caatcaggtg ttgaacctca aactgaaaca gtttggcgtc aagcaacaac
    33481 ttatggtgta cctcgtatcg tatttgcaaa caaaatggac aagatcggtg ctgacttctt
    33541 atattcagta tcaactttac atgaccgttt gcaagcaaac gctcacccaa tccaattacc
    33601 aattggtgcg gaagacgact tcacaggaat tatcgacctt gtaacaatga aggctgaaat
    33661 gtatacaaac gatcttggaa cagagatcga agaaacagaa attcctgaag aataccgcga
    33721 attagctgaa gaatggcgcg aaaaattggt tgaagctgta gctgaaactg atgaagaact
    33781 tactttgaaa tacttagaag gtgaagaaat cactgaagct gagttaaaag aaggtatccg
    33841 ccgcgcaaca gttaacgtag aattctaccc agtactatgt ggttcagcat ttaagaacaa
    33901 aggggttcaa ttactattgg acgctgttct tgattacttg ccatcaccgt tagatatccc
    33961 agctatcaaa gggattgacc ctaaaactga tgaagaagta gaacgtcctg cagacgacag
    34021 cgctcctttc tcagctttag catttaaagt tatgactgac ccattcgtag gtcgtttaac
    34081 tttcttccgt gtgtactctg gtgttttaca atcaggttca tacgtacaaa acgcaacaaa
    34141 aggcaaacgt gaacgtgttg gtcgtatcct acagatgcat gccaactctc gttcagaaat
    34201 ctctgaagtt tacgctggag atatcgctgc tgctgttggt ttgaaagaca caactactgg
    34261 ggacacacta tgtgatgaaa agagtctagt tattcttgaa tccatggaat tcccagaacc
    34321 agttatccaa gtcgctattg aacctaaatc aaaagctgac caagataaaa tgggtgtggc
    34381 tttacaaaaa ctatctgaag aagatcctac tttccgtgct gaaacaaacg tggaaacagg
    34441 cgaaacaatc atcgctggta tgggtgaact tcacttagat atcatcgttg accgtatgcg
    34501 tcgtgaattc aaagttgaag ctaacgtagg tgcgcctcaa gtatcttacc gtgaaacttt
    34561 ccgtgctggc actcaagccg aaggtaagtt tgtacgtcag tctggtggta aaggtcaata
    34621 cggtcacgta tggattgaat ttacaccaaa tgaagaaggt gctggtttcg aatttgaaaa
    34681 tgcgatcgtc ggtggggttg ttcctcgtga atacattcca gctgttgaaa ctggtttgaa
    34741 agatgctatg gaaaatggtg tcttagctgg ctatccatta gtagatatca aagctaaact
    34801 ttacgatggt tcataccatg atgtcgattc aaatgaaact gccttccgtg ttgctgcttc
    34861 tatggcatta cgtgcagcag ctaagaaagc taatccagta atccttgaac caatcatggc
    34921 tgtagaagtt gttatccctg aagattattt aggtgatgtt atgggtcacg taactgctcg
    34981 tcgtggacgt gttgaaggta tggaagctcg cgctggcggc caacaagttg ttcgtgcgat
    35041 ggttccactt gctgaaatgt ttggttatgc tacaactctt cgttctgcta ctcaaggtcg
    35101 tggtacattt acaatgacat ttgaccacta cgaagatgta ccaaaatcag tacaagaaga
    35161 aatcattaag aaaaacggcg gaaaagctga ataatttctt aaaaccggat agtcctgcaa
    35221 agagattatc caaagagact atatttttaa tatagtttcg tgtaaaataa ttatcgatga
    35281 tatgggcatg tggtgtacct tgtgtacatc atatacctat cagacaaata aaaaatatat
    35341 tgtattttta ggaggacatt ttaaaatggc aaaagaaaaa tttgaccgtt ctaaaccaca
    35401 cgttaacatt ggtactatcg gacacgttga ccatggtaaa actacattaa cagctgcaat
    35461 cacaactgta ctatctaaga aaaacggcgg ccaagctatg gcttacgatc aaatcgatgg
    35521 tgctccagaa gaacgcgaac gcggaatcac aatctcaaca gctcacgttg agtatgaaac
    35581 agatacacgc cactacgctc acgttgactg cccaggacac gcggattacg ttaaaaacat
    35641 gatcactggt gctgctcaaa tggacggagc tatcttggta gtttctgctg ctgacggccc
    35701 aatgcctcaa actcgtgaac acatcctatt gtctcgtcaa gttggtgttc cttacatcgt
    35761 tgtattcttg aacaaagtag acatggttga tgacgaagaa ttactagaat tagttgaaat
    35821 ggaagttcgt gacctattaa cagaatacga attccctggt gacgatgttc ctgtagttgc
    35881 tggatcagct ttgaaagctc tagaaggcga cgcttcatac gaagaaaaaa ttcttgaatt
    35941 aatggctgca gttgacgaat acatcccaac tccagaacgt gacaacgaca aaccattcat
    36001 gatgccagtt gaagacgtgt tctcaattac tggacgtggt actgttgcta caggtcgtgt
    36061 tgaacgtgga caagttcgcg ttggtgacga agttgaagtt gttggtattg ctgaagaaac
    36121 ttcaaaaaca acagttactg gtgttgaaat gttccgtaaa ttgttagact acgctgaagc
    36181 tggagacaac attggtgctt tactacgtgg tgttgcacgt gaagacatcc aacgtggaca
    36241 agttttagct aaaccaggta caatcacacc tcatacaaaa ttctctgcag aagtatacgt
    36301 gttgacaaaa gaagaaggtg gacgtcatac tccattcttc actaactacc gtccacaatt
    36361 ctacttccgt acaactgacg taacaggtgt tgttgaatta ccagaaggaa ctgaaatggt
    36421 tatgcctggc gacaacgtaa caatggaagt tgaattaatc cacccaatcg ctatcgaaaa
    36481 cggtactaaa ttctcaatcc gtgaaggcgg acgtacagtt ggtgccggtg tcgttacaga
    36541 aatcaaagct taattcactt aagttttaaa gtaaagcaac atttattcgg acgtaatcgc
    36601 tcggaacgaa ggtcttagga tctttgttcc gagctttttt atttattgca ggatcaggaa
    36661 aaaagacagg agtattataa atagaagaaa cacgaaataa aagagtcagt attttctaaa
    36721 tgaaacttta tgaaaacaaa atgtttttta caagaaaaag ccttgctttg tgagtttata
    36781 tatattataa tgacaaaggt gcgtttactt tagtgaattg cacagacgta atcagaatga
    36841 tttcgtcggc taagaaacga gaggttgcga cacgcccgga cgctttgcca cgaacgagcg
    36901 tgacggaaat tttcgtggag ctatgtctac ttttcaaaat aggcgaagga gggaacaaga
    36961 tggcaaaaca aaaaattcgt atccgtttaa aagcgtatga acaccgtatt ttagatcaat
    37021 cagcggataa aatcgtggaa actgcaaaaa gaactggagc tagcgtgtct ggtccaattc
    37081 cattaccaac tgaacgcagt ctttacacaa tcatccgtgc gactcataaa tacaaagatt
    37141 cacgcgaaca attcgaaatg cgtacacaca aacgtcttat cgacattgtg aacccaacac
    37201 caaaaactgt tgatgctttg atgaagctag acttaccatc tggtgtaaac atcgaaatca
    37261 aattataaaa ctaaaacaaa aataatggag gtgtactcat gaccaaagga atcttaggga
    37321 aaaaagtggg aatgacacaa atcttcactg aatctggtga attaattcca gtaactgttg
    37381 tagaagctac tccaaacgta gtactacaag tgaaaacaat ggaaacggat ggctacgaag
    37441 ctattcaagt tggttaccaa gatatgcgtg aagttttatc aaacaaacct gcgaaaggtc
    37501 atgttgcaaa agcaaacacg gctcctaagc gcttcattcg tgaattcaaa aatgttgagc
    37561 taggagaata tgaagtagga aaagaaatta cagtagatgt tttccaagca ggagacatta
    37621 ttgatgtaac aggtactacg aaaggtaaag gattccaagg ggttatcaaa cgccacggac
    37681 aaagccgcgg accaatggct cacggttctc gttaccatcg tcgtcctggg tcaatgggtc
    37741 cagttgcacc taaccgtgta tttaaaaaca aacgtcttgc tggccgtatg ggtggcaacc
    37801 gcgtaactat ccaaaacttg gaagttgtac gtgtcgatgc agaaagaaat gttatcttga
    37861 tcaaaggtaa tgttcctgga gctaaaaaat ctttaattac tatcaaatca gctgtgaaag
    37921 ctaaataatt gtaggaagaa aggaggaact aaggaatgcc gaatgtagca ttattcaaac
    37981 aagatggaag ccaaaacggt gaaatcacac ttaacgaaga aatcttcgga attgaaccta
    38041 atgaaagtgt tgtttacgat gcaatcgtaa tgcaacgtgc gtcattaaga caaggaacac
    38101 acgcggttaa aaaccgtagc gctgttcgcg gcggcggacg taaaccatgg cgtcaaaaag
    38161 gaacaggtcg tgcacgtcaa ggatctatcc gctcaccaca atggcgtgga ggtggcgtag
    38221 tctttggacc aacaccacgt tcttacagct acaaacttcc taaaaaagtt cgtcgtttag
    38281 caatgaaatc tgtattgtca gaaaaagttg ctgaaaacaa cttggtagca atcgaaggct
    38341 tgaacttcga cgcaccaaaa acaaaagaat tcaaacaagt tcttgctaac ttatctatcg
    38401 actctaaagt attggttgtt ctagaaaaag gtaacgactt tgctgcttta tcagcacgta
    38461 acttaccaaa cgtttctgta gtaacttctg ataatgtgag tgtgctagac gttgtatcaa
    38521 atacaaaagt gttagcaaca caaactgctc ttactcaaat tgaggaggtg cttgcataat
    38581 gaacttacta gacgtaatca aacgcccagt gatcactgaa aaatcaatgc ttgccatgga
    38641 tgacaagaaa tacacttttg aagtggacac tcgcgcaaac aaaactttag taaaacaagc
    38701 ggtagaagct gcttttgacg ttaaagtcaa aaacgtgaac atcgtgaacg tacgtgcgaa
    38761 attcaaacgt atgggcaaat atgcaggata tacaaaaaaa cgtcgcaaag cgatcgttac
    38821 attgactgaa gattcaaaag aaatccaatt attcgaagct gctgaataag cactaataga
    38881 taactcatta aattaggagg gaaaaacgtg gcgattaaaa agtacaaacc tacctcaaat
    38941 ggtcgtcgta acatgacagg ttctgatttt gctgaaatca caacatcaac acctgaaaaa
    39001 tcattgttac aaccattgaa aaaccatgct ggacgtaaca acaacggccg catcactgta
    39061 cgtcatcaag gtggcggaca caaacgccaa tatcgcgtga tcgacttcaa acgtaacaaa
    39121 gataacgttg ttgcaactgt tcaaacgatc gaatatgatc caaaccgttc agctaacatc
    39181 gcgttagtac actatgaaga tggagtgaaa gcttacatct tagcacctaa aggactagaa
    39241 gtgggtatga aacttgtttc aggtccagaa gcagatatca aagtcggaaa tgctcttcca
    39301 ttagaaaaca ttccagttgg tactgttatc cacaacattg aaatgaaacc tggtaaaggt
    39361 ggacaattga tccgttctgc tggtacaagt gctcaagtac ttggtaaaga aggcaaatac
    39421 gtattgatcc gcttgaactc tggtgaagtt cgtatgatct tagctgcatg ccgtgctaca
    39481 atcggttctg taggtaacga acaacacgaa ttaatcaaca tcggtaaagc tggacgctct
    39541 cgttggatgc gtaaacgccc aactgtacgt ggtagcgtaa tgaaccctaa tgatcaccca
    39601 cacggtggtg gtgaaggtaa agctcctatc ggacgtaaag caccagtttc tccttggggt
    39661 caaccagctc ttggttacaa aactcgtaac aaaaaagcta aatcagacaa acttattgtt
    39721 cgtcgtcgca aaaataaata attactttga ccatgtgtcg cacattttga gaggaggttc
    39781 accatgggtc gtagtttaaa gaaaggacct tttgtcgatg agcatttaat gaaaaaagtt
    39841 gaagctcaag caggggttga aaagaaaaaa gttatcaaaa cttggtcacg ccgttcaaca
    39901 gttttcccaa gttttgttgg atataccatc gcagtttatg atggacgtaa acatgtacca
    39961 gtttacatcc aagaagacat ggtaggacac aaattaggtg aatttgcacc tacaagaact
    40021 tatcgtggac atgctgctga cgataagaaa acgaaacgct aatttgaggg gaggataaat
    40081 ccaaatggca gaacaaatca catcagctaa agcaactgca aaaacagttc gcacttcacc
    40141 tcgaaaagcc cgtttagtaa ttgaccttat cagaggtaaa agtgtcgcag atgcaatttc
    40201 aatcttgaaa ttcacaccaa acaaatctgc tggaatcatt gaaaaagtat tgatgtcagc
    40261 agttgcgaat gcagaaaata actttgactt agatgttgaa aacttggtag taagcgaagc
    40321 ttttgttaac gaaggaccta caatgaaacg tttccgtcct cgtgcaaaag gatcagcttc
    40381 tccaatcaac aaacgtacaa gtcatatcac ggtagtcgta tcagaaaaat aaggagggat
    40441 aatcagtggg tcaaaaagta catccaattg gaatgcgtgt aggcatcatc cgcgactggg
    40501 atgctaaatg gtatgctgaa aaagagtatg ctgagttctt acacgaagat ttaagaatcc
    40561 gtaaatttat ttcaactaaa cttgctgatg ctgctgtatc aacagttgaa atcgaacgcg
    40621 caacaggtcg cgtaaacatt tcaattcaca cagctaaacc aggtatggtt attggtaaag
    40681 gcggttctga agtcgaaagc ctaagaaaag aattaaacaa attaactggt aaaagaatcc
    40741 atatcaatat cgtggaaatc aaaaaaccag atctagatgc aaaattagta ggtgaaggaa
    40801 tcgcacgtca attagaaaac cgtgttgctt tccgtcgcgc tcaaaaacaa gccatccaac
    40861 gctcaatgcg tgctggcgct caagggatca aaactcaagt atctggtcgt ctaaacggag
    40921 cagatatcgc tcgttcagaa ggatactcag aaggaactgt tcctcttcat actttacgtg
    40981 cggacattga ttacgcatgg gaagaagcag atacaacata cggaaaacta ggagttaaag
    41041 tgtggatcta tcgtggagaa attcttccaa ctaaaaaaaa cactgagaaa ggagggaaat
    41101 aatcatgtta gtacctaaac gtgtaaaaca ccgtcgtgaa ttccgcggaa aaatgcgcgg
    41161 ggaagctaaa ggcggaaaag aagtagcatt cggtgaatac ggtttgcaag ctgttgattc
    41221 acattggatc acaaaccgcc aaatcgaagc tgctcgtatc gcaatgactc gttacatgaa
    41281 acgtggtggg aaagtatgga ttaaaatttt ccctcacaaa tcttatactg ccaaagcaat
    41341 tggggtacgt atgggttctg gtaaaggggc acctgaagga tgggttgcac cagtaaaacg
    41401 tggtaaaatc atgtttgaaa tcgcaggcgt tcctgaagaa gtagctcgtg aagcgttacg
    41461 tctagcttct cacaaattac caatgaaaac taagatcgta aaacgtgagg aaatgggtgg
    41521 tgaatcgaat gaaggttaaa gaaatcagag aattaaccac tgccgaaatg cttgatcaag
    41581 aaaaacaatt aaaagaagaa ttgtttaatc ttagattcca attagcaaca ggtcaattag
    41641 aaaacactgc acgtattaaa gaagtacgta aatcgattgc acgcatcaaa actgtgttgc
    41701 gtgaacaagc taagtaatta tggaaggagg ccattaagcg tatgactgaa ggaagaaatc
    41761 aacgcaaagt ttatcaaggt cgcgtggttt cagacaaaat ggataaaaca attaccgttg
    41821 ctgtcgaaac aaagaaaaac caccctatct atggcaaacg catgaactat tctaagaaat
    41881 acaaagtgca tgatgaaaac aacacagcaa aagttggcga catcgtgaga attatggaaa
    41941 ctcgtccatt atcagctaca aaacgtttcc gtttgttgga agtagtcgaa gaagcagtta
    42001 ttatctaata acacgagatt ttcctatata gattgaatgc aaaatctgaa aggaggatac
    42061 acaacgtgat ccaagcagaa agtcgtttaa aagtcgctga taactcaggc gcacgtgaaa
    42121 ttttgacgat caaagtcctt ggtggatctg gtcgtaaaac tgctaacatc ggtgatgtga
    42181 tcgttgctac ggttaaacaa gcaacgccag gtggggttgt caaaaaaggt gacgtcgtta
    42241 aagccgttat cgttcgaaca aaatcaggag ctcgtcgtac agacggttca tatattaaat
    42301 ttgatgaaaa tgctgcagtg attattcgtg atgataaaag ccctcgtgga acacgtatct
    42361 ttgggccagt tgcacgtgaa ctacgtgaaa acaacttcat gaagatcgtt tcactagcac
    42421 cagaagtttt ataatcttga gacacttatc aaaggaggtg cgaaacagtt atgtttgtta
    42481 aaaaaggcga taaagttaaa gttatcactg gaaaagacaa aaacaaagaa ggcgttgtac
    42541 tagcagcgtt cccgaaaaaa gataaagtta ttgtcgaagg tgtaaatgtc gttaaaaaac
    42601 accaaaaacc ttctcaagct gctcctcaag gtggaattgt tgagatggaa gcgccaattc
    42661 atgtttctaa cgtgatggtg gttgactcta ctggtgtagc tggccgagtt ggctacaaag
    42721 aagtagacgg caaaaaagtc cgtgtttcta aaaaaaccgg tgaagtttta gataaataaa
    42781 agtttaagga aggagggtct tactgaatga accgcctaaa agaaaaatat cttaaagaag
    42841 taactccatc attgatggaa aaatttaact atagctctgt tatgcaaaca cctaaagttg
    42901 aaaaaatcgt catcaacatg ggtgttggtg atgcggtatc aaacgctaaa aacttagata
    42961 aagcagttga agaattaact ttgatcactg gtcaaaaacc aatgattaca aaagctaaaa
    43021 aatcaatcgc tggattccgt ttacgtgaag gaatgccaat tggtgcaaaa gttacattgc
    43081 gcggagaaag aatgtacgaa ttcttagaca aattggtttc tgtttcacta ccacgtgtac
    43141 gtgacttcca tggtgtaagt aaaaaagcct ttgacggtcg tggaaactat actttaggta
    43201 ttaaagaaca attgatcttc ccagaagtag attatgattt agtagataaa gtacgtggta
    43261 tggacatcgt tattgtaaca acagcaaact ctgatgaaga atctcgtgag ttgttgacac
    43321 aattaggcat gccattccaa aaataattaa aggaggcgaa ctacgtggct aaaaaatcaa
    43381 tgattgctaa aaacaaacgt cctgcaaaat tttcaacaca agcttatact cgttgcgaac
    43441 gttgcggacg tccacattca gtttatcgta agttccatct ttgccgtatt tgcttccgcg
    43501 aacttgccta taaaggtcaa attcccggcg tgaagaaagc tagctggtaa aaagtaaatt
    43561 cgcataagaa ggaggtaaaa gatcaatggt catgacagat ccaattgcag attttctaac
    43621 tcgtatccgt aacgcaaaca tggttaaaca tgagtcttta gaagtacctg catcaaaaat
    43681 taaacgtgac atcgctgaaa tcttgaaacg tgaaggtttc gttcgtgatg ttgaatacat
    43741 cgaagatgac aaacaaggcg tgatccgtgt tttcctaaaa tttggtaaaa acggcgaacg
    43801 tgttatcaca aacttaaaac gtatctctaa accaggtttg cgtgcttatg tcaaagctga
    43861 cgaagtacca aaagtattga acggtttagg tatcgcaatc atttcgactt ctgaaggtgt
    43921 tgtcactgat aaagaagctc gtgaaaaaaa tatcggcggc gaagttatcg cttacgtatg
    43981 gtaatcaagt agaatctaca aggaggtgtc tttaagtgag ccgtatcgga aataaagtcg
    44041 ttgttatccc tgaaggagta acagttactc aagacggaaa caacgttaca gttaaaggac
    44101 caaaaggtga attgactcgt actttttcag cagatatcaa aatgaatatc gaaggaaacg
    44161 aagtaacttt cactcgtcca aacgacagta aagaaatgaa aacaattcat ggaacaactc
    44221 gtgcgaactt caacaacatg gttgtaggtg tcagcgaagg tttccaaaaa gcgttagaac
    44281 taatcggggt tgggtaccgt gcacaattac aaggatcaaa acttgtattg aacgttggtt
    44341 actcacatcc agtagaaatc acaccaccag ctggagtaac tgttgaagta ccagctaaca
    44401 cacaagtaat tgttaaaggt gcgaacaaag aagaagttgg cgaattagct gcaaacattc
    44461 gtggaactcg tcctccagaa ccttataaag gtaaaggtat tcgttatgtt ggcgaatatg
    44521 tacgccgtaa agaaggtaaa actggtaaat aatcgtttag aaaaattgca tagaagccag
    44581 cagcaggaaa aaggcatttg gctgaagttt accttcaaaa aaatacattt ttacttgtgc
    44641 cgcagcttct atttttcgaa acgtatttac tgcttgaaat agtagtacaa taaaaataaa
    44701 gaggtgacaa ttgtgattac aaaaccagat aagaacaaaa cacgtcaaaa gagacatcgt
    44761 cgtgtacgta acaatatctc tggtactgct gagcgcccac gcttgaacgt ttttcgttct
    44821 aacaaaaaca tctacgctca aattattgat gacgtagcgg gtgtaacgct agcaagtgcc
    44881 tctaccttgg ataaagaaat ttcaggtgga acaaaaacag aagcagcaca agctgtcggt
    44941 aaactagttg ctgaacgtgc agctgaaaaa ggcattaaag tagtagtctt tgaccgtggt
    45001 ggataccttt accatggccg tgtgcaagct ttagctgaag ctgctcgcga aaatggacta
    45061 gaattttaga agaaggagga ataccattca tggtttatat cgatccaaaa catttggaat
    45121 tagaagaccg cgttgttgca attaaccgtg taacaaaagt tgttaaaggt ggacgtcgtc
    45181 tacgttttgc tgctttagtt gttgtcggtg acaaaaacgg acacgtaggg tttggtacag
    45241 gtaaagctca agaagttcct gaagcaatcc gtaaagcaat tgaagatgcg aagaaaaact
    45301 tagttgaagt acctatggtt ggttcaacaa tcccacacga agtaatcggt gcttttagtg
    45361 gtggtagaat cctaatgaaa cctgctgtag aaggttctgg ggttgctgct ggtggaccag
    45421 ttcgtgccgt attagaatta gctggggtag cagatatcac ttctaaatct ttaggctcaa
    45481 atacaccaat caacgttgtt cgcgcaacag ttgaaggttt gaaacaattg aaacgtgcag
    45541 aagaagttgc agaacttcgc ggcaaatcag ttgaagaatt aatcggataa ggaggacaac
    45601 aataatggct gaattaaaag ttactttaaa acgcagtatt atcggacgtc ctcaaaacca
    45661 acgcgataca gttaaagcgt taggtcttac aaagattaac agttcagtgg ttaagccttc
    45721 taatgaagca attaaaggca tgatcaatac tgtttctcac ctagtggacg ttgaagaaat
    45781 ttaatttaga aaaatcataa ctagaacatt aaggaggtgc caaacctatg aaacttcatg
    45841 aattaaaacc tgctgaaggt tcacgccaag tacgtaaccg tgttggtcgt ggtacttcat
    45901 ctggtaacgg aaaaactgcc ggacgcggac aaaaaggaca aaaagctcgt tcaggcggcg
    45961 gtgtgcgttt aggatttgaa gggggacaaa caccattgtt ccgtcgttta ccaaaacgag
    46021 gattcacaaa cgtgaaccgt aaagactatg cagtcattaa tcttgatgta ttgaaccgct
    46081 ttgaagacgg agctgaagta actcctgttg ctttagttga agctggaatc gtgaaaaacg
    46141 aaaaagctgg aatcaaagta ttagctaatg gggaactaaa caagaaatta actgtgaaag
    46201 cagcaaaatt ctctaaagca gctcaagaag caattgaagc ggctggtgga tcaatcgagg
    46261 tgatctaatg tttagactat taaaagatgc ttttaaagta aaagacatta gatcgaaaat
    46321 cctatttact gtatttgtgt tgtttgtatt tcgcctaggt gcacatatta ctgtaccagg
    46381 cgtaaatgct gcttcattgc aaaacctttc ttctttgcca tttttcaaca tgttgaactt
    46441 ggtaagtggt agtgcgatgc aaaacttttc cattttctca atgggggttt caccatacat
    46501 tactgcttct atcgtcattc agcttcttca aatggacatc gtgcctcgat ttgttgaatg
    46561 gtcgaaacaa ggggaagttg gtcgtaaaaa actgaatcaa gcgactcgat atctgacatt
    46621 agtattggca tttatccaat ctgtcggtat cactgctgga ttcaatcaat acgctcaatt
    46681 aggtttcgtg aataatccta acgtagcaac ttacgtaacg atcggtttga ttctaacagc
    46741 cggtacgatg tttgttactt ggctaggaga acagatcaca gaaaaaggta tcggaaatgg
    46801 cgtatcaatg atcatttttg caggtatcat ttctagattg cctgaaagta tcagagaaat
    46861 ttatgaagat tactttataa atattgatcc ttcagacata tggaaatctg ctatttttgt
    46921 ggccatttta ctagtagcgg tcttgttgat cgtgacattc gtaacatttt tccaacaagc
    46981 tgaacgcaag atcccgatcc aatatacgaa acgcgtagct ggcgcgccta caagcagcta
    47041 tctgccgttg aaagtaaatg cagctggagt tattccagtt atctttgcta gttcgtttat
    47101 tgctacgcca aatgctgttt tacaagcatt gagcggtaga ttcggttctg aaaactggta
    47161 caaagtgatg atggagatct ttaactataa tacagcacct ggtgctacga tttatacgat
    47221 tctgatcgtt gccttcactt tcttctacgc ctttgttcaa gtaaaccctg agaaattagc
    47281 ggaaaacttg caaaagcaag gaagttatat accaagcgta cgccctggta aggggactga
    47341 agagtatgtc tcaagattac tgatgcgttt aagtacagtt ggtgcagtct tccttggttt
    47401 agtggcattg cttccaatca ttgctcaaat ggtttgggat cttccacaat caatcggatt
    47461 aggcggtaca agcttactga tcgttatcgg tgtagcttta gaaactgcga aacagttaga
    47521 aggcttaatg ctgaaacgta aatatgttgg attcattgag taagaaagcg tgttgaggtt
    47581 tcccctcaac acacgttctc acacatttat taggagggaa aacaatgaac ctcattttaa
    47641 tggggctgcc aggcgcaggt aaaggaacac aagctgaaaa aatcattgat gcttatggta
    47701 ttccgcacat ctcaacagga gatatgttcc gcgcagcgat gcaaaacgaa acagctcttg
    47761 gcctggaagc aaagtcttat atggataaag gtgcattagt tcctgatgaa gtaacaaatg
    47821 gaatcgtgaa agagcgacta gctgaaccag atacagagaa aggcttctta ttagatggct
    47881 ttccacgtac gctagatcaa gcaaaagctc tggacgcaat gctaaaagat ttgaataaaa
    47941 aaattgatgc tgtcatcgat atccatgttg gtgaagaaat cttggtcgaa cgtctagccg
    48001 gccgatttat ctgcagcaat tgtggagcaa cttaccataa aattttcaac cctacaaaag
    48061 ttgaagacac atgtgatcgt tgcggtgggc atgaattcta tcaaagagaa gatgataaac
    48121 ctgagacggt taaaaatcgt ctggctatca acatcaaaaa tagtgaacca atcttggctt
    48181 attataaaga acaaggtttg ctaaacacta ttgatggtgc tcgtgaaatc gatgctgtat
    48241 ttgcagatgt acaaaaaatc attgaaaaat agcacgattg cccgtagccg acttgcatta
    48301 tgcagttact cttgtcaagt tcaataaatt atgatagaat atgtcagtcc gacttctaga
    48361 gaatcctcta gatgatccat tttgagaatt ttgaggtggg aactgcagtt tccgccattt
    48421 tatcgtgtga aaatgcgaaa caagtacaag gaggtactgt gcgtggcaaa agaagatatg
    48481 attgaagtcg aaggtacagt cgtcgaaact ttgccgaatg caatgtttaa agttgaacta
    48541 gaaaacggac accaagttct tgctactgtt tcaggtaaaa ttcgtatgca ttacatccgt
    48601 atcttacctg gtgacaaagt aacggttgaa ttgtctccat acgatcttaa ccgtggccgt
    48661 attacttatc gctttaaata attgtactcc gtaaaactac aggaggtatt atcatgaaag
    48721 taagaccatc agtaaaacca atgtgcgaac attgtaaagt aattcgtcgt aaaggacgcg
    48781 ttatggtgat ttgcccagca aatccaaaac ataaacaacg tcaaggataa ttggaggtgc
    48841 tataaatatg gctcgtattg caggagtaga tattcctcgt gataaacgtg tagtagtttc
    48901 acttacttat atctatggta ttggtaacac tactgctaaa aagatcttag ctgatgctgg
    48961 tgtatctgaa gatgttcgcg ttcgtgaatt aacgaatgaa caaacagatg ctatccgtgc
    49021 agaagtagat aaattaaaag ttgaagggga tcttcgtcgt gaagtgaact tgaacatcaa
    49081 acgtttgatg gaaatcggtt cttaccgtgg aatccgtcat cgccgtggat tgcctgttcg
    49141 tggacaaaac acgaaaaaca atgcacgtac tcgtaaaggc ccagctcgcg ctatcgctgg
    49201 caagaaaaaa taattcttaa gtgaaggagg ttaaaacttc atggcagcaa aaaaagtgaa
    49261 tcgtaaacgc cgtgtgaaga agaatataga agcaggtatt gcacatatcc attctacatt
    49321 caataacact atcatcatga tcactgatac tcacggaaat gcattagcgt ggtcatcagc
    49381 tggttcatta ggtttcaaag gaagcaaaaa atcaactcct tttgccgctc aaatggcagc
    49441 agaaactgct acaaaagcag caatggaaca cggattaaag actgttgaag taacagttaa
    49501 aggacctggt tctggacgtg aagcagcaat tcgttcatta caagctacag gtttagaagt
    49561 gactgcaatt cgtgacgtga ctccagttcc tcataatggt gcccgccctc caaaacgccg
    49621 tcgtgtttaa tgagtgccgc tcattttgag ttttatcagt aacgtcattg aacaagacac
    49681 ttttcgtttt gaaaggggta aagataagaa tgattgagtt cgaaaaacca agaatcgcaa
    49741 aaattgatga agaaaaagat tatggcaagt tcatcgttga acctcttgaa agagggtatg
    49801 ggactacttt agggaattcc ctacgtcgta ttttattatc ttctttgcca ggtgctgcca
    49861 tcactaatat tcaaatcgat ggtgtactgc atgaattctc taccatcaaa ggtgttagag
    49921 aagacgtcac tcaaatcatc ttgaacatta aaggcttagc gctgaaaatg tatggccaag
    49981 aagaaaaaac ccttgaaatc gatattaccg gacctgctac agtaactgct ggcgatatta
    50041 tcgttgatag tgatgtagaa attcttaaca aagatatgta catctgtagt gtctctgaag
    50101 gagctacgtt ccatgctcgc ttaacagtga aacctggacg tggttatgtt caagcagatg
    50161 aaaataaaaa agaagatatg ccaattggcg ttcttccagt cgattcaatc tacacacctg
    50221 ttcgtcgtgt gaactatcaa gttgagaaca cacgtgttgg acaccgtgaa gattttgata
    50281 aattgacgat ggagatttgg acagatggtt caattgaacc gttggaagca atgagtttgt
    50341 ctgcgaaaat catgactgaa catttggata tttttgtgaa cttaactgat gaagcaaaaa
    50401 atgctgaaat catggtcgaa aaagaagaaa cacaaaaaga aaaaatgttg gaaatgacga
    50461 tcgaagagtt agacttgtct gttcgctctt acaattgttt gaaacgtgca ggaatcaata
    50521 ctgtacaaga acttacaaat aaatctgagc ctgaaatgat caaagtccgt aacttaggac
    50581 gtaaatcatt agaagaagtt aaagcaaaat tgcacgactt aggtttaggt ttacgtaaag
    50641 aagattaaaa ccttttacga aggagggaaa ccaacgtgag ttatcgtaaa ctaggacgca
    50701 catctagcca acgtaaagcg atgttgcgtg atttaacaac tgatttatta atcaacgaac
    50761 gtattgtgac tactgaagct cgtgcgaaag aagttcgctc aactgcagaa aaaatgatca
    50821 ctttaggtaa acgcggagat ttgcatgcac gtcgtcaagc tgcggctttc gtacgtaatg
    50881 aagttgccag cgtgcgtgaa gaaaatgagg acatcgtcat tgaatcagct ttacaaaaac
    50941 tgttcaacga tattgcacct cgttacgcag aacgtcaagg cggatacaca cgcattttga
    51001 agacagaacc aagacgcggt gatggtgcac caatggttgt cttagaactt gtctaaaatc
    51061 acactaataa catcacttta tagatgattc ttttagagtg ttatgatgtt ggaagatatt
    51121 tttccgagtc tagctcagcg ctgctcctca agcgaatatc gcttggggag taagcactca
    51181 tcataaagtg tttttttata ccttcaatta cctgtcatga cagatatgtt tgaaggcata
    51241 aaaaaacatt ttaagcgaga aataattgaa aattttgtct ttttttgata gtgttagtag
    51301 gaactttagg atgaagaagg agtaatcatg gagccaatta ttgaattaga aaagataaat
    51361 tataaatacc agccagatga tcttcgcccc gcattgaaag atgtctcctt tacaattgat
    51421 aaaggtgagt ggatcgctat tattggacac aatggttcag gaaaatcgac acttgccaaa
    51481 acgatcaatg gattgcttct gccagaatca gggatcgtga aagtgggaaa tcaaatattg
    51541 gatgaagaaa atatttggac gatccgacag atggttggaa tggtttttca aaatccagac
    51601 aatcagtttg ttggttcgac agtagaagat gacgttgctt ttggtcttga aaatcaaggg
    51661 attcccagag aagaaatgct agtgcgtgtg aaagatgcac ttgagaaagt tagaatggct
    51721 gagtttgctt cacgcgagcc tgctcgttta tcaggagggc aaaaacaacg tgttgccatt
    51781 gccggcgtgg ttgccttacg acctgatatc attattttag atgaagcaac cagtatgcta
    51841 gatccagaag gtcgtgaaga agtgatctct acgatcaaga agatcaaaga agaaagtcaa
    51901 ttgaccgtta tatctatcac tcatgatatt gatgaagcag ctaatgccaa tcggatacta
    51961 gtgatgagac aaggcgaact tgtccgtgaa ggtacaccaa aagaaatttt ttctgctggt
    52021 cctgaactga tcgacttagg tctagatcta ccatttcctg aaaaactgaa gagcgcactg
    52081 aaagaacgtg gggttgatgt gcccagtgaa tatatgacag aagaaaggat ggtggattgg
    52141 ttatggacat ccgttttgaa caagtagact tcacttatca gccgaataca ccatttgaac
    52201 aaagagcctt atttgacatc aatatgacga tcaaagaaaa cagttatact gcattagtag
    52261 ggcataccgg aagcgggaaa tcaaccttac ttcagcattt gaatgcgctt gttaagccaa
    52321 caagcggtac agtccatatt ggagaacgag atattcagcc ggatacggat aataagaatt
    52381 taaagcccat ccgaaaaaaa gttggcatcg tctttcagtt tccagaagca cagctattcg
    52441 aagaaacggt agcaaaagac attgctttcg gtcctaaaaa ctttggtgtc agcgaagaag
    52501 aagcactagt cctagcaaaa gaaacattag aacaagttgg gctggatgaa agctatttgg
    52561 aacgttcgcc atttgaactt tcaggagggc aaatgcgtag agtagcaatc gctggtgtgc
    52621 ttgccatgag gccggaagta cttgtattgg atgaaccaac ggcaggactt gatccccagg
    52681 gtagaaaaga aatgatggag atgttttggc gtcttcataa ggaacagcag atcactattg
    52741 tattagttac gcatttaatg gatgatgtag ccaattttgc agattatgtc tatgtgttgg
    52801 aaaagggacg aatcgttaat agcggggagc cgcaagaggt ctttcaaaac attgaatggc
    52861 taaaagaaaa acaactaggc gtacctactg ccaccgagtt tgcagaagaa ttaatggcta
    52921 aagggatgaa ctttgctacc ttgcctttga cagctgaaga attagcagac gctatcgtcc
    52981 attatgctgg aggaaatgcc catgatgaat aaattgatat ttggtcgtta tatgcctgga
    53041 gattccttga tccatcgatt ggatccccga gctaaattat tagccagttt ttattttatc
    53101 ggaatcattt ttttagcaaa taattgggaa acgtaccttt tgttggcatt tttcacatta
    53161 ctttctgtct tcttgtcaaa agtagatttg aaatttttta tccgaggcat tcgcccattg
    53221 atctggctaa tcctcttcac tgtagctttg caggtcttgt ttacaagtgg cggagaaact
    53281 tattggagtt ggggtgtttt ccatatcact gaatttggtg tgatgaacgg cctctttatt
    53341 ttctgtcgat tcgtgttgat catttttatg tcgactctac tcacgcttac tacaccgcct
    53401 ttggaattat cggatgcgat tgagtacttg cttcgtccat tgaaagccgt acggttccca
    53461 gtccacgaaa tttcattgat gctgtcgatt gctcttcgtt ttgtgcctac attgatggac
    53521 gaaacagaaa agatcatgaa cgctcagcgc gctcgcggtg ttgattttgg tgaaggaaac
    53581 ctagttcaga aaatgaaagc agtcattcct ttgctgattc ctttatttgt cagcagcttc
    53641 aaccgtgcgg aagatcttgc gactgctatg gaagcaagag gctacaaagg tggggaagga
    53701 agaacgaaat atcgtgtatt gcattggcat aatagagaca cgatggttat cattgctttt
    53761 gtattattaa ctgtgctact gatctttttg agaggataaa cgttcagtgg gccgctatct
    53821 attcttttct tgctgatctg tttgcaagaa aggatgcgag cagtgtatga caagaaatat
    53881 tagggtctgt ggcagactaa ggtcacaggc ctctttcttt tatctgaaaa gaaagaacga
    53941 aaaaatttta taaaaaaatc attattccag ataacctttt aggaacaaag gagagaaaaa
    54001 catggtacgt tacaaagcaa tcattgcata cgacggaaca aattttaatg gattccaaaa
    54061 acagccaaat gggcgtactg ttcaagaaga agtagaaaaa acattacaaa aaatggcaaa
    54121 tggaaaagaa atcacggtct ttggttcggg aaggacagat gccggagtac atgcgattgg
    54181 gcaagtaatc cattttgatt atccagagga gcgtccgtta gaacgaatgc gtttcgcgct
    54241 agatacccag tcaccagagg atattgctgt aagacaagta gaaattgttt cagaagactt
    54301 ccacgcaaga tatttggtca aagaaaagac gtatcaattt cgagtggata tagggaaacc
    54361 acgcagtcct tttcgacgcc attatgcaag ttattttcct taccctcttg atttggaaaa
    54421 aattcagcgg gcgcttcctg atttattagg gactcacgat ttcacctctt tttgtgcatc
    54481 aggaagctcg atagaggata aagtacgtac gatctacgag gctaagatgg aggtaaatga
    54541 aacaaaagac gaattgttgt ttacatttag gggaaatggc ttcttgtata agatgatacg
    54601 gatcttagta ggaacgcttt tgaaaattgg caatggccga ttgcctgaag atagtatccc
    54661 agatattatt gcgaaaaaag atcgtaatgc agcaggacct acagcacatc ctgaaggact
    54721 ttatttgtat gaagtgaaat atgaataaga ggttgtgaca gaagtggtca gcttcaagaa
    54781 ataagaagga atttacgaaa attacttctc aaaattttgc aaaatttcgg cttattttcg
    54841 aaggagctac ttctggaaca ccgtgaatca gaggttgtga aaaagctgcc ctgcatcaag
    54901 taataagaac aaccaaacaa aaatagctcg tcatattttt cgtttggttg ggcttattac
    54961 cgcagatgca agcttttgaa caccgtttaa ggaagaggtt gtgaaatcag cgttttgacc
    55021 tgagtattag agaaacaaac ggaaaaatag ttcctcatat tttgccgttt gttgagctaa
    55081 taccgaaggt cagctgattg aacaccgttt aagaaagagg tcgtgagcct gtcatttaac
    55141 tctgagtcac aagtaacaat ttcaaaaaac agcttctcat ctttgatgaa tatcgtgact
    55201 tgtggacgag gcgttgataa gtgaatcatc tttttgttac ctattttttc tgtaaccaaa
    55261 aaacgatgga acgttgtttt gattgccctt aatgaggaca gtcggaacag gtttgtcatt
    55321 tttatattta aatggcacaa atttttctaa ttatttgtca tttagcgctg aaaatttttg
    55381 ctatttatct aaaaaagtga taactactta agatgaagca gaatatttac gccatacggc
    55441 atacaaccgc gaaaaatata aaaaaaagaa aacgattgaa cgcgtttttg cagtcgcaaa
    55501 ggaaaagcat ggaatgcgct acatcaagta tcgagacttg gaaaacaatc aggtgtatac
    55561 gctgattgtt tttgctgcaa tgaatctgaa aaagttgtcc acttggcagt tggaacatat
    55621 gtcttcatat cttttattat acaaaaatag caaaagcacc agtctacact cttagtaaag
    55681 aggaaactga tccttttgtc tacattctta aagtacttaa actaagtact ttattttact
    55741 ctttaggatt gatacagtag catctatccc tttattaata atcttttatt tttttaaata
    55801 aagatgttgt ctccatcttc aggcactaaa actttttcgg aaattccttt ttctttattg
    55861 aaagacttta attcttctct tgacagtccc caatggttta cagcttccat atgaacggca
    55921 ataattgtgg aatctggtat tgctttatgc aattcatata aatcgtcttt ccccataata
    55981 agagattctc caactaaaaa atgattatct ccagcatttg ctactatgat ttctggatta
    56041 tactgatcaa tagtttgttt aacagcttca taccaaactg tatctcctgt aaagtatagt
    56101 gtcttctcag tcgaatgttt gaaaacaata ccgcaaacat tacctacata attcaagatt
    56161 tctcctctgc catgtttgcc ttttgtttta accaattgta tagattcaaa aactgtgtct
    56221 tctcgtagta cctccacatt tttaaaacca tatagtcgaa tttcattcgc atcattttca
    56281 tcttgcacaa ataattttat ttctttaggt aggacctctt tcgctatatc gtcccaatga
    56341 tctagatgta aatgtgttac aataacagca tcaataccgt tgataatatc ttcgactggc
    56401 attggcaaat caactagagg attcatttga tcttttcttg gtgatggaaa aggacctttc
    56461 gaaataaatg gtggaagtgt tcctttttcc gctaacattg gatcaattaa aaatttttta
    56521 cctccatagt ctaagatagc tgttgcatta cgaatgtgat gtatattcat aaacgttgtc
    56581 tcctttttgt tgatatgaaa tatttttagt ataattcaat tccatacaat aaataaaccg
    56641 aacaaaaaaa gtcttttact atttttactt aatttatgaa aggttgttta ccatgttaga
    56701 ccaaacagat ataaatatca ttgaggaact atcaaaaaat agtcgaatca caatgaaaga
    56761 acttggagaa aaagtacatt tgactggacc cgctgtctcg gcgagagtga caaaactaga
    56821 ggaaagtgga gttattgaaa attatacagt aaaattaaat atggaaaagc tagggttttc
    56881 gattcacgct tttttaatta taattacgca aaatttaaat cataaaccct atttatcatt
    56941 tattaaaaat catgaaaaat tcttgattag aaattataag attagtggag ggggctgtta
    57001 tcttttggaa tgtagattta cttcaaaaaa atcaatgaac agctttttgg aacagcttaa
    57061 tgaatatgcg aattataaat tatctattgt tata
//
LOCUS       DAYYGO010000005        50050 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000005 DAYYGO010000000
VERSION     DAYYGO010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.005"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(22..1722)
                     /locus_tag="ACHA5T_000264"
     CDS             complement(22..1722)
                     /locus_tag="ACHA5T_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8885872.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            2229..3389
                     /locus_tag="ACHA5T_000265"
     CDS             2229..3389
                     /locus_tag="ACHA5T_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8885873.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            3405..3848
                     /locus_tag="ACHA5T_000266"
     CDS             3405..3848
                     /locus_tag="ACHA5T_000266"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8885874.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(4331..4861)
                     /locus_tag="ACHA5T_000267"
     CDS             complement(4331..4861)
                     /locus_tag="ACHA5T_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFM8885875.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            4966..5517
                     /locus_tag="ACHA5T_000268"
     CDS             4966..5517
                     /locus_tag="ACHA5T_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8885876.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(5670..6143)
                     /locus_tag="ACHA5T_000269"
     CDS             complement(5670..6143)
                     /locus_tag="ACHA5T_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8885877.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            6281..7753
                     /locus_tag="ACHA5T_000270"
     CDS             6281..7753
                     /locus_tag="ACHA5T_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFM8885878.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            7750..8460
                     /locus_tag="ACHA5T_000271"
     CDS             7750..8460
                     /locus_tag="ACHA5T_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFM8885879.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            8812..9096
                     /locus_tag="ACHA5T_000272"
     CDS             8812..9096
                     /locus_tag="ACHA5T_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFM8885880.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(9413..9796)
                     /locus_tag="ACHA5T_000273"
     CDS             complement(9413..9796)
                     /locus_tag="ACHA5T_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFM8885881.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIK"
     gene            complement(9798..10382)
                     /locus_tag="ACHA5T_000274"
     CDS             complement(9798..10382)
                     /locus_tag="ACHA5T_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFM8885882.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(10429..11904)
                     /locus_tag="ACHA5T_000275"
     CDS             complement(10429..11904)
                     /locus_tag="ACHA5T_000275"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFM8885883.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            12085..12396
                     /locus_tag="ACHA5T_000276"
     CDS             12085..12396
                     /locus_tag="ACHA5T_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8885884.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(12511..14847)
                     /locus_tag="ACHA5T_000277"
     CDS             complement(12511..14847)
                     /locus_tag="ACHA5T_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFM8885885.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            14989..15657
                     /locus_tag="ACHA5T_000278"
     CDS             14989..15657
                     /locus_tag="ACHA5T_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8885886.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            15660..16703
                     /locus_tag="ACHA5T_000279"
     CDS             15660..16703
                     /locus_tag="ACHA5T_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8885887.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(16940..17821)
                     /locus_tag="ACHA5T_000280"
     CDS             complement(16940..17821)
                     /locus_tag="ACHA5T_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8885888.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            17980..18708
                     /locus_tag="ACHA5T_000281"
     CDS             17980..18708
                     /locus_tag="ACHA5T_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFM8885889.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            18864..19181
                     /locus_tag="ACHA5T_000282"
     CDS             18864..19181
                     /locus_tag="ACHA5T_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8885890.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            19174..19782
                     /locus_tag="ACHA5T_000283"
     CDS             19174..19782
                     /locus_tag="ACHA5T_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFM8885891.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            19837..20628
                     /locus_tag="ACHA5T_000284"
     CDS             19837..20628
                     /locus_tag="ACHA5T_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFM8885892.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            20766..21254
                     /locus_tag="ACHA5T_000285"
     CDS             20766..21254
                     /locus_tag="ACHA5T_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFM8885893.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            21305..22177
                     /locus_tag="ACHA5T_000286"
     CDS             21305..22177
                     /locus_tag="ACHA5T_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFM8885894.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            22353..22715
                     /locus_tag="ACHA5T_000287"
     CDS             22353..22715
                     /locus_tag="ACHA5T_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8885895.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            22724..23476
                     /locus_tag="ACHA5T_000288"
     CDS             22724..23476
                     /locus_tag="ACHA5T_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8885896.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            23490..25292
                     /locus_tag="ACHA5T_000289"
     CDS             23490..25292
                     /locus_tag="ACHA5T_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8885897.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            25569..26609
                     /locus_tag="ACHA5T_000290"
     CDS             25569..26609
                     /locus_tag="ACHA5T_000290"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM8885898.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(26661..28805)
                     /locus_tag="ACHA5T_000291"
     CDS             complement(26661..28805)
                     /locus_tag="ACHA5T_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8885899.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(29162..30535)
                     /locus_tag="ACHA5T_000292"
     CDS             complement(29162..30535)
                     /locus_tag="ACHA5T_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8885900.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(30535..30930)
                     /gene="rbsD"
                     /locus_tag="ACHA5T_000293"
     CDS             complement(30535..30930)
                     /gene="rbsD"
                     /locus_tag="ACHA5T_000293"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFM8885901.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(30958..31875)
                     /gene="rbsK"
                     /locus_tag="ACHA5T_000294"
     CDS             complement(30958..31875)
                     /gene="rbsK"
                     /locus_tag="ACHA5T_000294"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFM8885902.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(31983..32996)
                     /locus_tag="ACHA5T_000295"
     CDS             complement(31983..32996)
                     /locus_tag="ACHA5T_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFM8885903.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(33077..33676)
                     /locus_tag="ACHA5T_000296"
     CDS             complement(33077..33676)
                     /locus_tag="ACHA5T_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFM8885904.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            33862..34563
                     /locus_tag="ACHA5T_000297"
     CDS             33862..34563
                     /locus_tag="ACHA5T_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8885905.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            34573..37284
                     /locus_tag="ACHA5T_000298"
     CDS             34573..37284
                     /locus_tag="ACHA5T_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFM8885906.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            37735..39363
                     /gene="treC"
                     /locus_tag="ACHA5T_000299"
     CDS             37735..39363
                     /gene="treC"
                     /locus_tag="ACHA5T_000299"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFM8885907.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(39423..40604)
                     /locus_tag="ACHA5T_000300"
     CDS             complement(39423..40604)
                     /locus_tag="ACHA5T_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8885908.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            40856..42379
                     /locus_tag="ACHA5T_000301"
     CDS             40856..42379
                     /locus_tag="ACHA5T_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFM8885909.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(42448..43176)
                     /locus_tag="ACHA5T_000302"
     CDS             complement(42448..43176)
                     /locus_tag="ACHA5T_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM8885910.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(43189..44469)
                     /locus_tag="ACHA5T_000303"
     CDS             complement(43189..44469)
                     /locus_tag="ACHA5T_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFM8885911.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(44610..45212)
                     /locus_tag="ACHA5T_000304"
     CDS             complement(44610..45212)
                     /locus_tag="ACHA5T_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFM8885912.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            45457..45663
                     /locus_tag="ACHA5T_000305"
     CDS             45457..45663
                     /locus_tag="ACHA5T_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8885913.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            45975..46694
                     /locus_tag="ACHA5T_000306"
     CDS             45975..46694
                     /locus_tag="ACHA5T_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM8885914.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(46775..47458)
                     /locus_tag="ACHA5T_000307"
     CDS             complement(46775..47458)
                     /locus_tag="ACHA5T_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFM8885915.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            47677..49863
                     /locus_tag="ACHA5T_000308"
     CDS             47677..49863
                     /locus_tag="ACHA5T_000308"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFM8885916.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT        16277 a         8857 c         9984 g        14932 t
ORIGIN      
        1 ggatgtattt atcccttttg cctaagcacg taaaagcttg tatctatgat aaataaataa
       61 acatagcaag gtaaaccagc caatcaaagt aagagaaaga atgaaataga aatagattgg
      121 cgtttgataa tgaagcgtga gctcgttgat tcctttttgc tgcttcacga ttggggtccc
      181 gatatctgtt acttcataat cagtcaatgt ttttccattt tgctgtagca cagtccgatc
      241 gtagacgata acagggatat gaacagtttt gtccccagtg tttttccact tgattgtcaa
      301 aagaccgcct ggatgtgttt ttataaaagt tgtttcgttt tctagtattt cttcgttata
      361 ccggtcgtat ttattgtctt ttgtctcctt atagattggc agatagtcag gtgtggtttt
      421 ttggaaaacg aacaagctgg tagacatatc tttgctgaag aatgttttcc ttgcttcttc
      481 cggcgtatcg aatagataag tatgtcgact gacaaatgtt tcattttccc acttttccag
      541 atgctggtat aagttctgga tcgtctgtgc tgaaaaaata agaatgccaa atatacttaa
      601 aagacggaac cacttttgtt cagaccactg gttcagtgaa agggcaaggt aaagtagaag
      661 caatagcgta aatggcacga aaaatcgaaa tggaaactgg atcaaactaa taaaaggcac
      721 atttttttcg atcaatgtcg tccatggaat caagctagta gaaagaaaaa agaaaacaag
      781 acttgtgtac aacacgattt tttggaataa ggtgttcttt tgttgaaaga aaataaaaag
      841 caaaaaacct gataatatca atataggaaa ggccttaggg taatggagcc aataactgcc
      901 atccagatct atcgcctttt gactcatttc tctgttaaca aagggaggaa gaatcttatt
      961 cccactgtaa acaatagcta atcctgccca aatatttgct gtcagaacaa aataaagcaa
     1021 gaccgactta atcagttgaa gcagtgcttt tcctttttca gtctggttaa aaaagtaata
     1081 aagataaaaa gggatataca tcaaagctaa catgatcgaa gacagtaaat ggatctgtgt
     1141 cataagtccc actgagacag cgactttgat aacagggaac tttttatgaa ggacgaaatc
     1201 agcaataggg atcatacata aaggataaaa acaagtcccc cagctagtga atccttggtt
     1261 gatagaccag tattgaatag aatacgtact catgaataaa acggctactg gtacacttac
     1321 ccaatttcga acattgacat atctcagtaa aatcagcata gaaaatcctg aaataagata
     1381 aagaatgaaa tttgaaagaa tctgataatg aaaccagcta cctgcaagca acacgatcaa
     1441 tccttgtaaa taagcgaaaa atgggccgta aacggcattc acgatcctgc cactttgctg
     1501 gaatccataa agggaaggaa aataatggaa atccccatat ttaatttgtt gcgcggcatc
     1561 atagaaccga ttgtaatgga atggggcatc agcacccaaa attagtttat gtgtgaataa
     1621 atgaggcatg agaaatgcca aagcaatgaa aaaaattgct aaaaatgggc catatttttt
     1681 caattgtcga actcctttgc aaactttaga taaattcatc atatatccta tgcgagaggt
     1741 aggcaagaaa ttagaaaatt catgaaaata tttatgaatt ttacaagaaa aacataagtg
     1801 aaagttctaa ggagagagga tgcgacataa gttttgttgc atctccttgt tttgaaacag
     1861 aatttttctt aatactcgaa acaaagtagc tttttcatga cctctcggta aacggtgttc
     1921 aatcagctgc ttctcggtaa taagctcact agcctagaaa atatgagaag ctattttctg
     1981 actagcgctc ttattactca aagcataacg cagttttcac aacctcttcc ttaaacggtg
     2041 ttcaaaagct tgcatctgcg gtaataagcc caacccaaca aaaaatatga gtagctattt
     2101 ttttgggctg tacttattac ttgatgcagg acagcttttt cacaacctca tcggctttca
     2161 ttttctattt ttttcctgaa cccatataat aaaagaaaga acaaaaaaac ttgcaaggga
     2221 ggacacatgt gaagcagagt aagcaaaaac agtctcgtcg acttttgttt cgtttgtttt
     2281 tattaatatg tatcgtcgta gggttactga tcgctttata tccattttat gtggattctt
     2341 taaatagttt tattgatcaa aaacgtatcg aacaagtaca aaaacggacc gcagctgaaa
     2401 ttgaagtgca gcgaaagaaa atggaggaga aaaaccaacg attaactgac caaggattca
     2461 atcctggtac tgatcctttc aatgaaaaga acagaaatga aagtacaaca tccagccaat
     2521 tagaggaatg gctgatcgga agtatcaata tacctaagat ccaattaaat atttcacttt
     2581 atgatcggct caatggtagg atccttgaaa atggagcagg cgtcttgcaa ggaacctctt
     2641 tcccgcttgg tggaaattcg acgcattcag tgatctctgc tcacagcggt ttacctaatc
     2701 gacggttatt taccgaatta gatcgtttag aaaacggaga cacgtttatt ttgactgttt
     2761 tgggggagaa attggcttat caagttaaaa atatacaagt ggtactgcca gatgacacgt
     2821 ctgttctaac gatttcagaa ggagaagact tagttacctt gcttacatgt acgccgtata
     2881 tgatcaacac gcatcgtttg ctagtgacag gacatcggat tccttataac gaatcggtaa
     2941 aaaaagaaga aggaaaagga aatcaggaaa gaaccatgcg gcaatggttt attcttgcag
     3001 gaacgataat agctgtagtc atactgttag tgtttatcgg acgactaatc tatcagtata
     3061 gattaagtaa aaaagtgctt gatttttcat ttattattag tgattcgtca gggaaaccgg
     3121 taaatggtag ttcattcatt ttgaaacata agaaaaaaac acttactaga aatggtgtgc
     3181 cgttttcagt acaaagcgac catcatggga aagtcaagct ggatcaattg cctggtggaa
     3241 cttatcgaat cgtttcagct gatgatccta aagttgctgt ttcttttggt attagaaaag
     3301 taaaacaaga gaagatatac ttttttgagg ggcgaaaact tgtaaaagag cttcaaaaaa
     3361 atggttttta ttttaaaatc aatgaataag taaggagaga aaaaatgatc cgcacaacaa
     3421 caaaattgtc tgccaaagaa ctggaatctg ttctatcgat ttggctgcat gctaacttgg
     3481 attctcatga attcattcca tcaacttatt gggagaatca agtagaatct gttaggaaag
     3541 ctttaccaga tgcagtgatt tacttagcag aaatagatca agaaattatt ggttttgctg
     3601 gggtgattga tcagtttatc gcaggaatct ttgtgaaaag ggaataccgg caacaaagaa
     3661 tcggtacgca actaatgaaa aaaataaaag aaaccaataa aacactgact ttgacagtct
     3721 atcaaaaaaa tcaatcagct attcatttct atctttcaca aggttttaca attcaaacac
     3781 agcaaaaaga tgaagcagta aatgaggagg aacttgtgat gaaatggaat caaaacgctg
     3841 atttttgatg aacagaataa cgaaataatg ctcccagcct aattttttgc tgggagcatt
     3901 atttcatttt gaagtcacta atttaactgt gtgaatatgc agttgttaca atctttgata
     3961 aacggtgttc aatcagctga ccctcggtat tagctcaaaa aacggcaaaa tatgaggaac
     4021 tatttttcca tttgtttctc taatactcag gtcaaaacgc tgatttcaca acctcttgat
     4081 aaacggtgtt caaacggctg cttctcggta ataagcccac tagcctagaa aatatgaaaa
     4141 gctattttct aactagcgtt cttattactc aaagcataac gctgatttca caacctcttg
     4201 ataaacggtg ttcaatcagc tgaccctcgg tattagctca aaaaacggca aaacatgagg
     4261 aactattttt ccgtttcttt ctctaatact caggtcagaa cgctgatttc acaacctctc
     4321 tttcacaacc ttattttact cgtttataat tttcatcgaa gtattttccg ctacggatat
     4381 catctggcat tccatcgtat ggttcttcgc taataacatc ttcattgtct atacctgcat
     4441 cacccatata agtgatcgta gcaaattcag gaacaagtaa ttttggataa gtttcatatt
     4501 tttcacgagc tgcttccatt tctgcgatat gttcgttttg gaaagtaaca gtgatttctg
     4561 gatgttcttc aacccattta gggaaaaaga tcattccgtg aagtttcttt tcatttggca
     4621 taaagtccaa tgccacgtct gtccctgttg gctctgtcca agtgattttg aatacgccgt
     4681 ccgtcaattt aacgatatca gcttcttggt cttttaccca acggcctttg accattccgc
     4741 catggatacg gtaatcaaca gtatggtcat ttttggcata ccattcatat tcccaaccat
     4801 tgtcatacgt atagataaag tgagttccaa taaaatcttc caattcttta aattctttca
     4861 tgtttaagca tctccttata aattaaaaca tgtaataaac tacatgtttt cgattactca
     4921 tttatgatag aatggattta aagatttgtc aaggagggaa aactgatggc aagaaaaaat
     4981 acacttcgat atattttgtt agggttatta agtaagaaga agatgacagg atatgaattg
     5041 aatcaatcgt tcaaaaatga aataggagaa ttttggcaag caaaacatag tcagatttat
     5101 cctgaacttg caaaaatgga agagcaagga atcattcagc atcaagtaga gatcacaggt
     5161 gaaaaattgg aaaagaaagt ttatgaatta acagaagaag ggaaggaaca attggcagag
     5221 tggattcata cacccaccaa tgaactaccg gttaatcgag acgaatttgt tctgaaattg
     5281 tatttcgtca aagacgtgaa tgatccggca ttagtagaaa ttattcagca gcagcgtgac
     5341 cttcatgaag aaaaacgact tcatctatta gagcgaaaaa aaacgatctt tcctactgaa
     5401 aaagaaaaac atgaaaatta cggacactac ctgattttag cgcatgcgat caatcgggaa
     5461 acagaatatg caaattggct aacagatgta ttagaagaaa gtaaccgaaa aaaataaaaa
     5521 aatatccgaa cagtgatgga gaattccttt ctatatgaaa gaaacgcaga tcaccgttcg
     5581 gatattctta tgttcaaaca ggcctttttt caagtattta tgtagcatct acttattaaa
     5641 aagcttggta cttaggagat tagtggcgtt tagagttctt ttaacaactg ttcgtagtca
     5701 gctttttcat cgacaggaag gagcttgtaa gttaattcgt ctgtctcgtt gtcatgaatg
     5761 atgtacatct tagcattctc ttctttgaag ttaccgattt gtaaaatcgt atagcgttcg
     5821 tcgatcaatt cgatatcttt gattgctttt gaaaaaactt tacggacatg gtgcattttc
     5881 ttttggcgga tttctgcctt ttctgcagct tttgacaaag taaatccgtc atctaaatat
     5941 tttttgatca gttctacttt tatgactgta ggtaaacgat attttctagg acaattcgca
     6001 tcagcttcga tcgaacgaat gaatcccttt tgttcccaat accttaattg acgtggagac
     6061 acacctacga tatcacttaa ttcactgatg ccaacaacca attgatcatt ttccaacaaa
     6121 cgtttaaggg aagccccaac catttttgtc actccttttg tcctcttgct tcccttaatt
     6181 atatccgtat taacttatgt gtcaagtagg ttgacaaata gcgaatttag gactaaagta
     6241 ttctagtagt ttcagaaaaa attcagaaag aaggagaaat atgagtaaca accaacccgt
     6301 agatatttac ggaaaaccat ataatcggac acttttagtt gttgtcttat taattggtac
     6361 attttgtacc gttttgaatc agaccttatt gacgacagct tttcctacat tgatgaaagc
     6421 tttcgatatt tcagcgtctg atgtgcaatg gttaacgact ggtttcttgc tagtcaacgg
     6481 gatcatgatc ccaataacag catggctgat caataaattc agttcaagaa atttatatct
     6541 ttctgccatg acgatctttt tgatcggaac gattacttgc tttacagcac ctaattttag
     6601 tacattacta atcggtcgtt tgatccaagc ttgcggtgtg ggagtatcga tgccattatt
     6661 gcaaaatatc atgctatcta ttttcccgcc agaaaaaaga ggatcagcaa tggggatgtc
     6721 agggatcgtt attggacttg ctcctgcttt aggccctaca ttatctggtt atatcattga
     6781 taactaccat tggcgtgact tgtttggtat ggtactgcca atcgttgttc ttgtcttggt
     6841 tttagcattc ttcctaatga aaagtgttat tccattgtca aatcctagca ttgatatttt
     6901 atccgctatt ttatcaacga ttggattcgg tagtttactt tacggatttt ctagtgtagg
     6961 agatgacggc tggggaagct tgaaagtaat tggtttctta gttgttggtg tgattttcat
     7021 cgccttgttc tcatggcgtc aattacattt ggaacatcca ttcttggaac ttcgtgtatt
     7081 caaatcatct acattcacga ttgcagctgt tttagctggt gtaacaaata tggcgatggt
     7141 aggggcagaa atggtcgtac cattgtatat tcaaaatatt cgtggagaat cagcattcca
     7201 ttctggcttg atgttgttgc cgggtgcatt gatcatgggg gctatgatgc ctgtgacggg
     7261 tgcgattttc gataaatacg gcgcgaaacg tttagccatc acagggatgt tcattttgac
     7321 agcggcaact gcaccatttg ctttcttgac aaaaacgact ccagttttat atatcgtgat
     7381 cctttatgcg atccgtatgt ttgggatctc catggtcatg atgccggtaa cgacttctgg
     7441 tatgaatgca ttgccaaata acttgatcag tcatgggaca gcggtaaata atacattccg
     7501 tcaaatcgca agctcgattg gtacagctat tttgatcagt gtgttgacga atgtgacaaa
     7561 agcaaatatc ccaagcaaat ctgtattgga taatacgcca ttagcttata aagatcaggc
     7621 aatcaatgcc acactgtcag gttaccatgc cgcgtttatc gtagcgatca tctttgggat
     7681 tcttggcttt attattgcct ttttattgaa gaaaaaagaa cctgttctaa tgaaagcggg
     7741 ggataatgca tgattattgg agttttgatt gtcagcttgc ttgcttttgc tttaacgaac
     7801 gtgttcgcta aaaaaacatg gcaaaccttt ttatctttga ttttcggact aatcttcgtc
     7861 gcttcactaa gtttgatcgt cgctaatttg tccaatcact ttggaatgga aaaagtaacg
     7921 gaaacaaaaa cagaaaaaat tgtttccagt gctgattccc aaggagcaga tatgcttttg
     7981 tataaagctt tagggaatgg aaaagaaaaa gtgtatcttt accgaacaaa tgaaaaacaa
     8041 gaaaaaccaa aagcaactgg tacggataac gaaacaaata aagtagaaaa gacagacggc
     8101 gatgctgaaa aagtgacgaa aactacttat tgggaataca aaaacgacat gtataaattc
     8161 tggttcaata ttgctgataa caatcacgaa tatgacagcc gagtgaatac attcaaaatc
     8221 ccagagacat gggtagaatt aagtacggac caagcagcca agttagctga attagtgaag
     8281 aaacagcaaa gtacgatgga aagtgaagca aaagcttatg ttcaagacgg tatggtaaaa
     8341 gcaatgactg aaaatccaaa aatgagcaaa gcagagcaag aacaacgtac gaaagcctta
     8401 gctgctgaat tccaacaaca agcattcgca aaattagtga aagaagcaaa aggcgaataa
     8461 tattttgaac accatgtagg aggttgtgaa aaagctgctc tgcatcaagc agtaagaaca
     8521 atcagataaa aatagcactc catatttttc atctgattgg gcttagtgcc gtagatgcaa
     8581 gcttttgaac accgtttaag gaagaggttg tgagcctgcc gtttaactct gagtcacaag
     8641 gagcaatttc aaaaaacagc ttctcatgtt tgttgactat tgtgacttgt gaccgaggag
     8701 ttggcaggag aacaccgttt attcgaaaaa aggaactgcg acaagacttt tgtcacagtt
     8761 ccttcttttt tttgctatgc tatactagat gtaaaaaata ggagaaataa aatgagggaa
     8821 atttattctt ttgaatcaaa aatctatgct tccgaagtcg gcaagggcgg ggcatatatt
     8881 atttttccgt atgatataag aaaagaattc ggtgctggaa gagttaaagt ccaagcaact
     8941 tttgacaatg tgccgtatga aggcagtatc gtgaatatgg gcgtgaaaaa tccagatggt
     9001 tcagtctgct acatccttgg actacgaaaa gatattcgta aaaacatcgg aaaagaaatc
     9061 ggtgatattg tagcagttac agtgaagcaa aaataaaaga ggttgtgaca aaagacgtgt
     9121 cacaacctct tgataaacgg tgttcaaaag ccagtttctc ggtagtaagt caaatttatg
     9181 caaaaatgtg gaaacccatg tttacaaaat ttttcttact actcgaaacg aagcggcttt
     9241 atcacaacct cttgataaac ggcgttcaat cagctgtccc tcggtattag ctcaaaaacg
     9301 gcaaaatatg agaagctatt tttccatttt tttctctcat actcaggtca taacgctgat
     9361 ttcacaacct cttctttcat aatggttttt caccatcttt ataataaact ggttatttga
     9421 tatggaaatc tattttttcc aaccaatcta aaatcttgta acctaactct tctgacatcg
     9481 gaaaagaggc gttgatttgt gcattggaga tatggtcaac atggataccg gaagtgatcg
     9541 tacaacttcc cggaacggca gttttgatga tcacagctat tttttcggct aagacatcgt
     9601 ctttatggaa ccgaccatcg tggctaggga aacgtacagt ttgcatctct gtatcacgag
     9661 aaaatgtagt gactgttccg atatgtggtg tatctccgcc tgttaaagtg atcaataagt
     9721 ctttaccgat tactttaaca gcagcttgga tcgtgaaacc tagtttggtt acttcaacta
     9781 tcgtttcttt ttccatatta atcaccctgc caacgttttg aatgctcatt tacaatttca
     9841 aattgatcca aaagtttcat tgtatggtga ttaacaagat cttggatcgt ttgaggctga
     9901 ttataaaaag caggaattgg tggtatgatt tgcacaccta gtcgtgctaa tttagtcaag
     9961 ttttccaagt gaatggcatt taatggcgtt tcacgaggaa caatgactaa ctttcgttgc
    10021 tccttcattg ttacatcagc agcacgtgca atcaggttgt cgcctaaacc aacagagata
    10081 cctgcgactg ttttcatact tgccggtacg atgatcattc catcggtcaa aaatgatccg
    10141 ctggcgacag cagcacctaa atctttgttt gaataatggt aatcaaacag gctttcgaat
    10201 tcagttagtg ataagtcgga ttccagcttt aagttctgtt ttgcccatgg actgatgatt
    10261 ccgtgtgttt ctatcatcgg atattctttt agtttcttca ctaggttgat ggcataaatg
    10321 gtgcctgaag cgccacttac accaatcacg atttttttct tccgttgtgc caattgttcc
    10381 attttccgca ctccttttta tgatgtcaaa acagttgcag atcattcatt atttcaaata
    10441 tttttcccaa tcttctactt cttggaattt tgcacgtaca aaatgttctt tcatatcgaa
    10501 aggaactgta ccgtcaaaga tagtttttgc acttatccca cggaaacgaa tagagccagg
    10561 attatattct ggtaattctg atgggtccaa tggatggttc ctcattcctg ggatcacgat
    10621 gatatcttta tctccttgga aacgagtgtt gaaagtccaa actacatcgt tcatgtcgaa
    10681 aatatcaaca tcttcatcga ctaaaatgac tgtcttcaat tctttgaagg ctgataaagc
    10741 aagaatagct gcttgacgct gaataccttc atccgcttcg ttttctttac ggatttgcat
    10801 gatggtcatc aatttaccac cacctgcagg aggattgtag acatttacta cttttcctgg
    10861 aatagcttta tcgaccaagg caagaatact tgcttctgta ggaataccgg ccatagatac
    10921 gtgttcttca ctgggaccaa tcgttgtttg catgattgga ttgtcttttc ggtgagtaac
    10981 tgctttgact ttcactactt gtacagctgg attagcatcc ccagtatagc caggaaattc
    11041 tggcattgct tttcctgagt tcgtgttgat atcttcttgc attgtttcat ttggcatgat
    11101 ttctgcttca atgatgaatt cagcacgagc aatacccact tcatcgactg ctataccatc
    11161 aacgatttga ataggttcgt tacgcaaagc tcctgcacac catagttcat tgtagccaag
    11221 tggagtggta ggtggttcga aagtcgtgcc aatggcaatt gctggatcta aaccaatatt
    11281 gatagtgata ggcattggtt tattcaaggc ttcgaattgt ttttggaagt gaccaatgtg
    11341 gcgtccgcca ggcatgatgt acataccgat cgtatcttta tcttctagaa ccatacgatg
    11401 gatcgttaca tcgctcattg ttttttctgg atctgatcct aagactacac ccattgtgat
    11461 gtatggacca gcatcttcag gagtatttgt aggtgcaggc aaaatgttgc gaatatcgaa
    11521 gtctgcatcg gtagctagat gaacgacttc ttgtgctgga gcgtctgctt tgtctacttt
    11581 cacaggtttg attggatttt ctacagcgtc tttcaagaaa cgaccaagtg ttcgataatc
    11641 atggtgcaaa atttcaccta cacgtttacg actagccatt gttccgatgt tcacacgtgt
    11701 gccggggaaa cctttgatat tgttgaaagt catagctggt ccttcttgag tcggacgttt
    11761 tactgtaccg ccagcaccga tataacggta aacaccagat aattctgctt caggatcgat
    11821 ttcaatatct gtttcgtgat attgacctgg aagtgttttt agttcttcga tgacttttct
    11881 taaatcatac ggttgatctg tcatgagaga ttcctcctta gttgtcttta cctatctata
    11941 gtaaaaggtg aaaaagagga aaacaattca acttgttcgg taaagtattc tacaatggaa
    12001 tggatgcgtt tttttgataa tagaataaac tatgatatac ttatcactaa aagaaagcgc
    12061 atacttagtg ttggaggaag gaaagtgaaa aaaatggagg agtcatttcc taaagcagta
    12121 aaagtggaaa atatagcgaa tattttgaaa gttacatttg aaaacggaga agtaaaatac
    12181 gtgaaatctc attggacaga agagataacc gatgccttgc aatttggaaa aaaaggaaga
    12241 ggaaaaagaa aaaatcttct cgcactttct cgtaatatgt ggattgggac tgaagtaacg
    12301 attgaagcag atggaacagt attcataaat ggaaaagaca ggtatacgcc agaagagttg
    12361 tggtacaaag gaaagaaaag tataccagaa ttataaaaaa taattaaaaa aaacagcgcg
    12421 aaacaaggaa catattactt cctcgtttct tgctgttttc tactatttca gcgctcttat
    12481 gactcaaatc taaatgtggt tgtcacaacc ttattctgca cgcaaagcga tagcggcgtc
    12541 ttttttcgct gccattcgtg caggaagatg accgccaagc atcgtcaata tagtagagac
    12601 gatcactaga ataatcgcat ggacaggatt tagttgagca acatttttta gatccgtcat
    12661 gttgtataag atgctattga tagggaacgt agctagccat gcaataaaca cacctaatat
    12721 cccagaagaa atacctaaaa tacacgtttc tgcgtcaaat acgcgagtga tgtctttttt
    12781 acgtgcacct aaagctttta gtacaccgat ttcttttgtc cgttcaatca cagatgtata
    12841 agtaatgata ctgatcatga tcatacttgt tactaaagag atccctgcaa aagcaataag
    12901 gacataagtg atagcatcca tcaatccgcc agtcagttca gtcatcgttc cagctaaatc
    12961 agtataaatg attttatctt catctgattt gcctttattg taatcatcta gataatccaa
    13021 gatcttttct ttgtcttcaa aattgttcgg ataaatcatg atacttgaag gcaagctgtc
    13081 tccacctaaa taactaagca atgtttgttt ggcattttcg tcaacttttt cagtagttaa
    13141 gacatttaca ccactgtctc tttgagcttt gacgatttct gattctttgt tttcgttgac
    13201 gatttgagtc gttaattgat cgctataagc gattcctgga gataagagat tcattgtaga
    13261 agaagatttc acgcgtagga tccccgagat cttcaattca tcagaagcat tctgatacat
    13321 cgcatcataa tctgtattag ggataaaatt cccagttgga agttttgtat agtaagtatt
    13381 gttgtttact aatttgaatg ttgtaccaac gatatcatca aaatcaagtt tttcgtcttc
    13441 tttcacatca aatccaagat tcttcaatgc gttgatatta gtattattat tgccgtctac
    13501 gatcagcacg acatctgttg ctgaggctgg ataacttccg gcaagtaaag aataattgtc
    13561 ttttaaaaag ttttctttgc tagtgtcaag ctgtgtaggg aaacttgaca ctcccacacc
    13621 tgtcattgct gacatcgtac tagataaaga aagagattcc gtatcaggat tttgattcga
    13681 gaagctgaca ggctgtactt tgccatttac atcacgtaaa agattgattc ctgtagtacg
    13741 ggtaaatccg atattattac ttaagttagg atcgatgtct gttacgtaat cgacgtatgt
    13801 ttgatcaatc ttattcgtgt gctgtgcttt atcttcttcg ctgacttttg cagtaatcgt
    13861 tttgctgtct ggataatccg ctttgcttgc acccaatccg gcatcgtcac gagtctggtt
    13921 agtcgttacc ttagaaatag taatcggaaa tttcgccatc gtttcagctt gcgtgttatc
    13981 gatttgtttt tggaatccag aagaaaggga cagaacgatc gcgattccga tgatcccaat
    14041 actggaagca aaggaggtca agaaagtacg tccttttttt gttcggatat tgttgaagga
    14101 aagctttaac gctgtccaaa aactcatctt tgttctgcgt aagttaaatt gatcatcttt
    14161 tggtcgttcg atgtgaggat gggaatctgt taagatctta ccatcagaaa attcaatgat
    14221 tcgatctgcg tattgatggg ccagttcagg attatgtgtg accatgatca ctaatttttc
    14281 tttcgataac tcttggatca attccatgat ctggatactt gtttctgtat ctaacgctcc
    14341 ggttggttca tcgcacaaaa ggatatctgg gtcattggct agtgctcgag cgatggccac
    14401 tcgctgcatt tgtccaccag ataactgatt tggcttttta tgcatatgat ttgtcaagcc
    14461 gactcggtgc aatgcatctt ctgcccgttt tcgtttttcg tctttagaaa caccacttaa
    14521 tgtcatccca agttcaacgt tttcaataat gcctagatga ccaatcaagt tgtaactttg
    14581 aaaaatgaaa ccgatagagt tgtttcggta ggcatcccag tcactgtcct taaagtcttt
    14641 agtagacttt ccgttgatta ccatatcacc ggaatcataa ttatctagtc caccaatcac
    14701 attcagcata gtcgttttac cagaaccact aggacctaaa atcgctacga attctttttg
    14761 tctgaaagcg acagacacac catccaatgc tttggtggtg gtttcgccga ctttataata
    14821 tttttttata tcttttaatt gaagcataat cacccttctc ccgtattgaa atattcggta
    14881 tactaataag tatatcagaa caatgatcgt agtatacgaa ataaatgtta actgaagatg
    14941 aactacaaga aaaaatctag aaatttttca aaaaacagag ggagatcaat gaatcgtatt
    15001 ttagtagtag aagatgatcc gcaattacag ttgctttacc gttctgtgtt ggaacgagcg
    15061 ggcttttcag tcatcaccgc attgaatgga gaggatgcgc tgaaaaaact agaagcgaca
    15121 cgtatagaat taatcattac agatatcatg atgccgaaaa tggatggcta tgacttgcta
    15181 gactcattac gtagcaccag atttgacaca cctgttctaa tgattacggc aaaggccgat
    15241 tttgaggata agaaacgcgg gttcaaatta ggagcagatg attatatgac aaaacccatc
    15301 gatgtcaatg agatgatcct acgtgtggaa gcattgcttc gacgttcaaa gatcaattac
    15361 agcagtagct tgataatcgg acaaacaaaa ttagagctag atgcttatac agtgttacaa
    15421 gatcagcatt cgttgattct gcctcaaaaa gaatttctgc ttctgtataa attactgtct
    15481 tatcccaaca agatcttcac tcgccaacag ttgatggatg atatttgggg attagatacg
    15541 aatacagaag aacgaacgat cgatgtacat atcaaacgtt taaggacccg ttttgaaaag
    15601 acggaagatt ttcaaatcat cacagtacgg ggactaggct ataaggcggt tatttaaaga
    15661 tgaaaaaaaa gctattttca caattatgga tctattttgc tagtattgtc ttcatttcga
    15721 tcttagtgac actgctgtgt tttctcggtt ttattttctt attgtctcga cattctatct
    15781 cgcctgctga atcggctgga aaaccaacgt tatacccgtt attcgtcttt gcggggttca
    15841 gtatggtcgt gggtacagga atatcgattt ttgttgggcg gcgtatcctg catccaatca
    15901 gtgcgctagg gacaaatatg agtctggtag caacgggaga tttttcgatt cgaatggatg
    15961 aacagcagaa agtagcagaa gtacagcaac tgtataaaga tttcaacgtt atggtccaag
    16021 aactcaatag tatcgagaca ctgaggaatg actttgtgtc cagtgtatcc catgaattca
    16081 aaacaccact agcaacaatc caaggatatg ttcaattgtt acaagcccca aatctctctg
    16141 acgaagaacg ccaaatattt ttatatcgga ttatcgaaag tatcacgcag ctttcacaat
    16201 taacagagaa catcttaaaa ttgaacaaac tggaaaacca gcgtatccaa ctggaaaaga
    16261 aagagtatcg attagatgaa caaatcagag aagtaatcgt tttttttcag cctaaatggg
    16321 agaaagaaca gctagaattg gatatcgaac ttgctgcagt aaattacaca ggcaatgaag
    16381 agtttttgta ccaagtgtgg ctgaatatca tggataacgc cataaagtac aatcaaatca
    16441 atggacagat tcatatcaaa ctatttgaga cagccacaga gattgtactg gaagtgaccg
    16501 attcaggtgt aggaatgaat gaagagacca gagatcgaat gtttgaaaaa ttttatcaag
    16561 gagatacaag tcgtcaaatt tctgggaatg ggctaggact ttcactggtt aaaaaaattc
    16621 tagaactgca tgatggacgt attgattata gcagtatcga aggagtagga acgacagcaa
    16681 tgatacggtt aagtaaacaa taggaaaaag aataaaaccc ctaaaaattc tattgaaggt
    16741 ttttaggggt ttttagtcgt ttttttgtga ttagggaaat gttcttacgg ttcttttggc
    16801 ataaaaatcg ctgctttctt attgaactac gagaaatgtt agaggtgtca ttgaaacaag
    16861 gacatgatgc aagaaagaga gcgaaagtca tgcgtcgctc tctttttttg ggtggataga
    16921 tggacagaaa atgtagggct tagttttctt cttgatttaa atactcccaa aatgtatctg
    16981 aagccaaaga atgctcaccg actttacgag taaatgcaag ataactctct gttgttggag
    17041 aaatagggag aatcacagta ttttctttta atagattctt gactgttttt tccataacaa
    17101 tagcaatccc catgtcattt gcaatcatat tgatgatcaa gtcgattctt gctcctttat
    17161 aagagatttt tggttcaaat ccagcttcgc gacacagatc tagtacaggt tgatacaagt
    17221 tcgtagtggg gccaaggagc aaaaagttct cctttttcag ttcgcttaga tccaatttct
    17281 ttttactagc taaaggatga tttttaggta gaactgctac aaaatcgtct gtttcagtag
    17341 gcaaccactc aatcgaactt ggtggggcct ggaaacttct agcaaagtgg atcgtgtcac
    17401 tatgttcatt ggcgtcattg taagttaatt cgacactttc cacttctttc aactggactg
    17461 taaattcagg atggcgtttc aaaaactcgg tgattttgga aaaaccttta taattgatca
    17521 ttgtcggtat cgttaagata tttaatgaaa gattgttgtc tttcgtatag gtttcaagca
    17581 cttcttgcat agcatggtat tgttctacga cattttttac atatgggagc aataagcttc
    17641 cagcttcggt caattcgatt tttctatggg cgcgttcgaa taatttgacg tctaattctg
    17701 tttctaacga aagaatctgt ttagaaatat ttccttgggt ggtgaattgt cgatcagcag
    17761 ttttggtgaa attcattgtt tcggctaaat caagaaaaac ttcgaattta tggatatcca
    17821 tcatattcct cctacttatt ccaataaaga atactttaac acaaaaaatg aattggtgta
    17881 atcttctctg tctgttacaa tctttcttgc agcgaaagca ataattacag aaatttttct
    17941 gatttttttc gcttcgtaga aaaagagagg agttttttga tgccaactgg agttttgatc
    18001 aatgtaggtt ccgttttgtt tggcggacta attggcggat tagtaggaaa taaattaagt
    18061 gaacatttta aagcacagct gacgatggtt tttggtgtgt gttcgatggg gatgggaatc
    18121 tattccattg cgccgatgaa aaatatgccg gctgttattt ttgctttagt gataggaaca
    18181 gcgattggtt tgattgttca tttaggtaat gggataaata agggggctgc gctgatgcaa
    18241 gtacctattt ctaaaatctt ccctagtgaa aagcttggta tgacccacga tgaatttatc
    18301 agcacattag tgacggtcat tgttctattt tgtgcaagtg gtacggggat ctatggaagt
    18361 ttagattctg ggatgacagg agacagtacg attttgattt ctaaatctgt ccttgatttt
    18421 ttcacagcag ctatttttgc gtgtaattta gggtatgtcg tttctgtgat agcaattccc
    18481 caattcatta ttttttatat tttgttctta ttggcaaaat ttatttatcc attgactaca
    18541 ccagacatga tcttagattt taaagcttgc ggcggattct tgatggtcgc taccggtttt
    18601 agaatgatca acgtgaaaat gttcccagta gctgacatga tacctgcaat gatcgttatc
    18661 atgccactga gctggatgtg gacaaattgg attatgccac tgttataata aaaatcaaag
    18721 agaagccatt ttttgctgac taaaaaggat caaatctttt agtcagtttt tttgtatttg
    18781 acaaactgat tttgaaacta tattatatgt tgtataatat agattgaaat aaaatattac
    18841 acttgagaat agaaggtgac gtaatgcaca tccagattcc aactgttctt cttgatggta
    18901 ctgtgctagc agcattaagc aaggaagata tgtatgggta tgccttaaca aaaatcgtac
    18961 aagaaagtct ctctgtcagt gaatctacac tatatccggt tttgagaaga ttgaaaaaag
    19021 aaggatatct tgagacctat gatgaacctt ttgaaggcag aaaccgtcga tattactcgt
    19081 tgacaccaat gggaagtcgt catttagaag aaattattga tgaatggatt gtttttcgag
    19141 attctttaga ccggttattg gaggagagca caaatgaatg aatatttgaa tgaagttgtg
    19201 gatcatcttc aagaaattcc tgaagacgat cgttttgatt tgatccagta ttatgaggaa
    19261 tattttttag attctggaaa aacattagat cagatcattg atgaatatgg tacaccaaag
    19321 cagtttgcct taaaattaaa aatcagttac ttttcagaag aagaacaagc agaaaacgag
    19381 ttagtgaaaa atccaaaaag acaaattcgc ctgatttggt taatcattat cggtttattt
    19441 gcttctcctg tcctcattcc actagcactg gggtttatat tggttattgg gggactattg
    19501 gttgctttac tagcgctcat ttttagtatc tatgttttat gtatcagtat cttaggtgtt
    19561 ggcctattca tgatcatctc aggaatcagt gtgtttggac aatcgattct gagtggatta
    19621 ttctttatcg gtgtcggtct gttcgccaca ggaagcgcag tatttatttc gccactgttg
    19681 ctgaaaggaa caaaagggct tttttatgtc atgatgaact ttgttaaatg gatgggacga
    19741 cgttttatta caaaaagaga tctgcattca gcgggcgttt gaaggaggac acataaattg
    19801 agattgaaac attttttgat taccggtatt ggtctgatgc ttactggtgg cctcctagca
    19861 ggtgcaactt atacattagg cgctcaaaag agtctgactt gggataatgg tcctcggttg
    19921 atcgatatgg ttactgagac aaaaaaaatc aaggatgcaa acaaaatcgt catccatgca
    19981 gaaaatcaaa cagtttcggt catacgcgga ttggattttg agataaaaac gacttatgat
    20041 aagacagaaa agccagaaat aaaggagaaa aatggcgtgt tgactgtgaa tgcagtcaaa
    20101 aaatcaacag gtgcaatcat tagtttagaa gacacaagtg cacaaatcac tattgttgtc
    20161 cctgcagatc atgaattatc tgaattgaca atcgaaggga ataatagtgc aattgatatc
    20221 aacgaagtat caatcaaaaa agtgaatact aagacacatg atggctgggt atcgttcaat
    20281 gaatttacag gaggaacaat aactgcgaaa gatgaattag gctcacttcg attagatgag
    20341 gccaatgtgg ataacttgat cgtcacaggt gaaaacagca acttccatct agaagacagt
    20401 ttgatcagca aagaaggaaa agtcatccta aacggtggca atttatcttt gcatgagatg
    20461 gaaaacagtg gctatactat ccaaacgtct ggtgaatctt atattgaaaa agactaccag
    20521 ccaataacaa atactttgtt agaacaagga cagaaaaagc taaagatcac tgctaaagac
    20581 acatcgatca atattacgac ggatcaagag gacaatgaaa cagattgatc ctgtactcaa
    20641 ctggatattt taagaaaaaa cagcaaagat ccttccaatc tttgctgttt ttcatcgctt
    20701 aacgcacata actatagtaa aataagaaag aaaatatcta aaaagaaaac agggtgaaag
    20761 agtatatgaa ggtagaagaa ggtcaaagag aaaagttaaa aacagaaatg gaaagattac
    20821 atacttatat cactcaacta agccaaacct tctatgatcc agacaaagaa aaagtgatgg
    20881 ttaattaccc gaacaattca gaaggtcgcc aattagagca agtgtaccat gaagttttca
    20941 aacatttatt gacggtgaag aaagagctgg attattattc tttaccgatt atcgatacag
    21001 gaatattgaa gtatgatggg aagaaggaac gatttatctt taaatctgtt cgggaagatt
    21061 tgcctctttc agcagggatg gatctagagg tcttggtaga agactatttt acagaagaaa
    21121 agcactgggt tcgaacaaaa ttggattatt taccgcaggc agccggaggg acacaggcaa
    21181 gcggttggta catcactgaa gacaaagagt tagaactaga aggtgtaatg gcgcgtattc
    21241 gaaaaaaaaa ttaaaaaagt aacattagat atttattgaa ggataaagga gcgcaggaag
    21301 tagcatgaga gaaaaaagag cattttatat caatggttat gcagggattg tcggattggt
    21361 cgtattagct ttagttggat tgtttttctt ctatttaggt atgtggcaag cgaaagtttg
    21421 ggcacttttt ttaagtattt ttctttggtt gatcacgtta ttgttgttaa gttcggcaac
    21481 tgtagtcagt cctaatcaag caaaagtaat tttgtttttt ggacaatatt taggtacgat
    21541 tcgagaaaat ggctttttcc ttacgatacc tttggcacaa aaaatgactg tttcgttgaa
    21601 agttcggaat ttcaatagtt ctgttttaaa agtcaatgat ttggatggaa accccatcga
    21661 aatctcagca gtagtagtct ttaaagtcgt tgatacagca aaggcattat tcgatgtagc
    21721 gtattatcaa gattttgtag aaatccaaag tgagacagcc attcggcata tcgctagcca
    21781 atacccgtat gatacattca atgagaatga tttgacgctt cgaggaaaca cgacggcagt
    21841 gtctgacgag ttgcaaaaag agctgcaaga gcgtttagca gtagcaggtg tcgaagtaat
    21901 cgaaacacgc ttgaaccatt tagcctatgc aacagaaatc gctagtgcca tgctccaacg
    21961 tcaacaagca aaagccattt tgtctgctcg acaaaccatt gttgaaggag ctgtcacaat
    22021 cactcaaatg gccttagagc aaatcgaaga tggacaagaa atcaatttca ccgatgatcg
    22081 gaaagtccaa ttgatcaata atttacttgt ctcaatcatt acagacaaag gaacgcaacc
    22141 tgttatcaat acaggcgaca tgaaagaccg taagtgacat actttttgta agataggaag
    22201 aaggagttta ggacggatta ttgtcttgaa ctcttttttt agttgtttta aagaaaatga
    22261 caaaattgaa agaagaatga tagttgattc gaaggcagaa ttgttcgctt ttttctataa
    22321 tcagtatata aagtaagaga gaaggggaaa aagtgaaaaa acttctgatc ggtctacttg
    22381 ttttcatcgt tgtggtactt gcaggggtga aaatcgcaga ttacgtagtt atgggtggag
    22441 aagaatacta tgtacaaatt acgacagatg gtacaaaaac agtttcaaaa tcagactctg
    22501 gacaagaaac agtcaactat agatatatcc tgcctggcta tacgaaagag ggtaaggaaa
    22561 aagaactgga cttcaaggga caattgactc gaccgcttcg taaaggagca tatttaaaaa
    22621 tcacctggaa caaaaataaa ggtgtcactt cgtatgaaga agtcgaaaaa aaagacgtgc
    22681 cgaaaacagc actggaaaaa ctaattaaag ggtagggaaa gaaatgaaca aaatagtcga
    22741 agtacagaat gtaacaaaag tatacggaaa aaacaatgag aaaaagacgc aagcattaag
    22801 cggcatcagc ttcgatgtag aaaaaggcga atttattggg atcatgggcg cttctggttc
    22861 tggtaagtcg acacttttga atattttatc cacattagat aaaccaacag atgggcatat
    22921 ccgaatcaat caaaaagatg tgaccacatt aaaaggaaat caactagcag attttcgtgc
    22981 gaatgagatt ggctttattt tccaagattt caatttatta gaaagcttga cagcacaaga
    23041 aaatatcgct gttcctcttt ctttacaagg cgtcagacca aaagaaatca agcaacgtgt
    23101 ccaaaaaatt gcagaacgtt tatccatctc tcatattttg gaaagctatc cttctgagat
    23161 ttccggtgga caaaaacaac gggtcgccgc cgcacgtgca ttgatcacac aaccaacgat
    23221 tctattaggg gatgagccta caggtgcgct ggattccaaa agtgcccggg atcttttaga
    23281 tacgatggac gaattgaata caaaagatca tgtatcgatt cttcttgtga cgcatgatcc
    23341 gttttctgca agttattgcc aacgtatttt attcatcaag gatggcggta tccatcaaga
    23401 agtgaaacgt ggagatcaaa gccgtgattc cttctatcga gaaatactat ctattcttgg
    23461 aaacttggaa cagtaggggg agattgacta tgctttggaa attatcactt actgggatca
    23521 agagccgttt aagagactac ttggtgttat tctcaggatt agttatggca tctgctattt
    23581 tttacatgtt tgaatcaatg gcaagcaacg aagctttttt gaaaagtaat acgatcgttg
    23641 cggctacagt tatcattttc cgattcggtt ctgttttact agggatcatc acattcgttt
    23701 atattttata tgcaaattcc tttttgatga cgatgcggca aaaagactat gcaatgttta
    23761 tgatgctggg agcaaaaggc agaaaaatcg cacagatgat tttttctgaa acattcttag
    23821 tagggatcgc agctacttta gtgggttcag ctgtcggtgt aggattgaca agtatcgtcc
    23881 accgattatt agtggatcag ttagatctca atatttcgca ttttacacct tttagtattc
    23941 acgggttgtt gatcactgtt ttgttttttg ctgttttgtt tttattagct gctgttgtca
    24001 atgctttttc gattgtcaaa aaaccgattt tagcattgat tcgtgcggat cagacaccaa
    24061 caagaatcaa gcagcataaa ttcttgtttt tccttgaagt agtactggga attgcttttc
    24121 taggctgcgg ttactatatg atgaaacacg tggggaccta tcaagtcttt ggtatagggg
    24181 tagccttagt tacgatcgta ttaggaactt atttcatttt ccattctgtg atcattttct
    24241 ttttatcatt actaaagaaa tcagaacaga tttcattgaa aaaattgaac aatttcacat
    24301 tgtcacagtt aaactttcgt attcgtgaat atacacaaat gctctcaatg gtcgctatgc
    24361 tgtttgcctt ggcactgggc gctttgacgg taggattagg ttttaaaaat gaaattgcag
    24421 acttgacaga aaacaattct ggatatgatc tagtgatgaa taatcctcgg gcagaagatc
    24481 agcaaaaaat caaagaactt tcaccaacac tttctgcttc ttatactcaa aaagaagatg
    24541 cgggaacgat ttactataat gcaagtgaat ttgcggctca accattgatc atgataaaac
    24601 atgaagatgg aacaccgccg aagatcacga aagaaaaagt tcctgctaaa aaaatgaatg
    24661 aactgactgt acaagaggaa cttcgaagct atgaattgcc ggaacagcaa gaaaaagaaa
    24721 tcaaggtagt cagtcaatca gaatttgatc aactgaactt gccacaatcc actttacagc
    24781 tagttcaggt aaaagacttc caagcaaacc tgaaaccaat cgaaacactt gttgctcaaa
    24841 ataaaatgaa caatccatct ttgcaaaaag tagagtacag caaccagaaa tatgaaatgt
    24901 atgaagtgta taatggtcta ttctctggtt ttgaatttat gggattcttc ctaggtttag
    24961 ctttcttgac catgcttgct agctgtttga tgttcaagat cttgtcagga agcaagagtg
    25021 acattgctcg ttatgaaatg cttgaaaaga taggaacacg acgcgggttg cttaaacaat
    25081 cgatccgtag agaagtaggc gtgttattct tagcaccagg aatcttaggt gtagtccatg
    25141 tcttattcgg attacaaatg tttggtctat tcatgcaaaa tccatacaaa gatatttggg
    25201 ttccgtttac gatattcttc gtattgtatt tcatctacta tgtactgacg acatggttat
    25261 atactggaat cgtattgaaa aagagattgt gaaaaagctg tcctgcatca agtaataaga
    25321 acagccaagc aaaaatagct cctcatattt ttcgtttggt tgggcttatt accgcagatg
    25381 caagcttttg aacaccgttt gttcggaaaa agaggttgtg acaatatttt tgtcacaacc
    25441 tttttttcac ggtcttttat tagaatatag taaatttttt ctcgttactc acggaataag
    25501 ctgttaagaa aacggtttcc atgtatactg gaatcagtgc aaatgaaaga aaagagggtg
    25561 caaggaacat ggaaatcaat gaacaaaatt ttggcgaaaa ctatcacttg attacgatca
    25621 cgaatcaaaa tgggacatct ttgtctgtca gtgatcttgg ggcacgggtt gttcattttc
    25681 aaacaataat cgataatgaa aaacgagagt tgatcttagg ttttgatgaa gcaaaagaat
    25741 atctagaaaa agacccttat atcggtgctt caatcggacg tatggctgga cggattacga
    25801 atggcacatt caaattagat gatgaaacgt tcgaagtaaa aacagacccg gacactggtc
    25861 attgtttaca tggtggtgca cctagctttg aagccaagca gtggcattat acagtcatca
    25921 atggtgaaaa cgaagcatcg gtcattttct atcagactag cccagatatg gaaaacggat
    25981 ttcctggaac tttagatgta gaagtacggt atacgctgac gaacgatgat acatggcgag
    26041 tgacggtcca agggatcagt gaccgcaaaa cagtattcaa tcctacaaat catgtctatt
    26101 tcaatttaac cggtgatcca gcacagtcaa tcgacgaaca tatactttgg ttgaatagcc
    26161 gattatttgc tacgctcaat aaagatacaa cgcctacagg agaagtagtt tcagtaagtg
    26221 gcacagcatt tgacttccaa cgtcctaaaa aattaggtga ggtctttgcc agtgattttc
    26281 gccaatcaat gatagtgggc ggcatcgatc atccattttt gttgaaaaaa caatctggtt
    26341 tactaaatgc tgcggaattg acaagcccag atgaaaaagt atcgatccaa gtaaaaacag
    26401 atgcttcagg aatcgtgatt tttacagcaa attttggaga agatggaccg gaaatgcgcg
    26461 ggaataaatt agtaaatcat ggcggtatca catttgagac tcaagaactg ccaggagcgg
    26521 agcgacttga aagttttgga gatgtcactc tggaacctaa ccaagtacgc gagtttgtaa
    26581 cggaatacaa aataaatata aagaaataga ataaattaaa aagggagtgt gacagtgtca
    26641 catccccttt ttaatttatt ctatttcttt aaaggcagat caagccagaa tgatttgtct
    26701 aaacttaaca aggttagtac aggaacagtt gcagctttaa ctgtacgagt agttgtttct
    26761 ggtatctgtc ttgccgtaaa attgttccac aaatcccaga ttgaagcgac acggaagatg
    26821 gaagaagcgt tgttgacgcg ttttatcgtt gaaaattcta atcctgtgat tggcacgcca
    26881 tctgttgtcc atttgattgg cacatcgaat cgcttagaca tattgaggat ttcagcgttt
    26941 gcttcttcag ttgttgctgc ttcttttttc ggttcatcat attttgtcag gtcgtacgct
    27001 tcgatgatcg cgttcaattt ttgtgaatat tggcgatctg tagcataacg tccttccaaa
    27061 tatttggttg cttctttgta gtcttttgtt tccgacttcc aagttggttt gtagaaatca
    27121 ggattgccag tcagtccatt tttgatcaag tcggcatagt cttccaaact ttccttgtaa
    27181 gaaggatatt tacggaattg tgcgcggatt tggtagcttt gtccggaact atcttgttca
    27241 agtgtattga aggtcacgct gcttccttta tatgatcctt tgataccaaa aaggttatga
    27301 tttggttcgc ttgataataa gctgtttcca gatccacttt caaggatagc ttgagccatc
    27361 atgactgatg cgtagattcc ttctttttgc ccgatttcct gtgcatcatc tgcaatcttt
    27421 ttcaagaact gatcagttgt ttggttttga ctgaaatgga acgagccatc taatccatct
    27481 aaaatagatg agccgcctgt cagcgaacct gaagcagcag gtaaacctgc taagtttact
    27541 cggacagcaa aatcaggcgt gaaatctgaa atagaagtta ctcgaacttt atctcctggt
    27601 tgaggcgcat ggataaattg tccgttccca atatagatac ctacatggtg agtaacaccg
    27661 gaagaaggtc cccagaagac gaggtcgcca ggttgggcct gacttactgg aatctgcgta
    27721 cctgcatatt gctgttcgcc tgtccagcca ccgatataat gaccggttgc tttcatatat
    27781 acgtaataag tcaaaccaga acagtcaaaa gtatttggac cttttgctcc ccatacatat
    27841 ggttttccaa gatgcttgaa tgcttctcga acgattgctt gttggacatt cgttgtattt
    27901 gtaggcaata cagcatcaat tggtgatttt acaacaaaat cagaagaagc agaagatgga
    27961 ttctctatac cgctttgatt tgctgtattt ggacgagatg aagcattgtt cgttccgcca
    28021 ttcgatgaac cattgtttgg atggtttggt tctctctgag tatttccatt gttattcgat
    28081 ccagatgggt ttgtttgatt cgaagaattc ccgctgttcg aattattgtt attattgttt
    28141 gaattatttg aaccattatt attgttgtta ttacccacat ctggtttgtt tggcgtttcc
    28201 ggatctggtg tactagggac ttcttcacta ggatcagttg gtttctctgt tgaagtatcc
    28261 ggtccagctt taaattctgc agtaatagat aaattgttcg tacctactgt aaaggtcttt
    28321 gcatcataat ttacttcttt ggcaccatta acagcgactg aagcaatttg ttcaccttca
    28381 gctggcgtaa tagtgtaagt taccttcgtt tccgctaata aaccttctac cgttccactt
    28441 tccgtaatat ccaaaggttg ctgttcaggg gaatccgctg tcgaagcagt cagttggatc
    28501 ttaccaatca aagaaggagc aattgtaagg gaatatgtct cggctggctt ttcattttct
    28561 gatggatcag ccggatcaat ttcagttggc tcggtcgatc ctgttgttga gttagttgtt
    28621 ccagttgggt cttctgtaaa agaatccgtt gtttcactag tagctgagct ggatgtagtc
    28681 gtatctgttg tgactgtttg atcagctatt tgattatcta cggcaaatac ggtattggtc
    28741 gacaaaacag acggagcaat cagccaagta gcagctagca aacgtatcgt ttttttattt
    28801 ttcataataa actagtcctt ttcctttcat tgattttctg tcttattcag cattcattag
    28861 acattttttg gaacagataa tcgtgtcgaa aagctttttc ttttcataaa tctgtctgtg
    28921 tattctaaat aaaacaattt gtaaataagc aacgagcatt cattcgtttt tctttgacgt
    28981 tcattttatt gcatagacca cctttgtttg gggataaaca agtggggata aaaatggtta
    29041 tgcatatgta aattatacca aagtttggaa agtatcacaa aaatttataa aaataatgat
    29101 cctaaaaaga aaaagactga aatatgtgat aaacatctca tacttcagtc tttttcatta
    29161 actattcata agacattagg tcttcatcaa acccgctaac gagagtagtg atcattgcta
    29221 cgactagact aacagccagt cctgctaagt accagaaaaa agtaccggaa atgaatgcag
    29281 gcaatgtggt cagactacca aatacaaaag cgatcgcttt cacttgggca aatcccatta
    29341 cagctccacc agcagctgca ccaatgcaag cagcaacaaa tggttttttc aatgggatcg
    29401 caattccgta aacagttggt tcggtcactc ccgcaagtaa aatcgaagca aaagcagaac
    29461 cggagatcga acgggttttt gaacgtttac ttcgcagaaa tacacctaaa gctgcaccag
    29521 ccattccaaa attggctgct cctgtcaacg gaacgattcg gtcgaaccca taagcagaga
    29581 tattactgat catgacaggg tttacagccc attggatacc aaatattacc aaaatattcc
    29641 aaccaccagc gatgattgcg ccactgatcg gtccgttaat atccatcatc caagtaattg
    29701 cggcagctaa accttcgcca aggtaaacac ccgcaggccc gaataccatt aacgtcaaag
    29761 gaaccgtgat aactaatgaa agtaaaggta caaatactaa ttgcatattt tcgcgaatat
    29821 attttttgag gaatttttca acaaaagaat aaaagaaaat acctaaaatc gccggcatga
    29881 cagtagacgt atactgcatc agtactacag gaatccccag aaaatcagta gctgctttgc
    29941 cactgtctga aagtaaagag gtcagattcg gttcaatcag tgctgcagca atgacagccg
    30001 caatatatcc attcacttta aacgttttgg cagcagaaaa agcaagaaag attggcagaa
    30061 aataaaagac ggcattcgac gcagcagaca aaatatgata agttccgctt gtttcactaa
    30121 gccagccaat cgtagtcgct aaaacaacca gaccttttaa tataccagaa ccggcaaaca
    30181 gcggaatgat cggcgtaaag atttccgata tcaaaccaaa tagtttttcg atcggattta
    30241 gcttttcttt cggagctgaa tgatcgattt gacgcgtcaa ttccttgtaa gcaggagcca
    30301 atccctgatc catgattatt tctgcatatc tatcgagtaa aatgaaatac ttaatggaag
    30361 ggatttgctg ttttaatttt tctgcattgg ctactggctg ttttttcaat ttgatacgaa
    30421 gccgagtcgc acatggaagg acagaatcaa tattgtcttt tcctcccaac tgttcaatca
    30481 gcatatgaat ctgctcggac agtaccatct gttcaggatt cattgtcgtt tccattagaa
    30541 gaccactcca ctcactaata taatattggc gtaaggtgtc atttcgcctg tacgaataaa
    30601 tgcatgacag ttattcaact cttttttcat ttcttcatgt gggataaagc taatcggtgt
    30661 atctggtaac aataatttga ttttttccaa tatttccggg ttcattgtct ttatttcttc
    30721 cgccaattca atcgattgta cctcaagttc ttctaaaacg ttttctaaaa cagataaaaa
    30781 acttggaaga ccgtttgaga cagccaaatc tattttttca gtaccaaagg ggacaggcat
    30841 gcctgcatcc ccgatactta atttatcaaa gtgccccatt tgtgcgatta ctcgtgaaat
    30901 atctgaattg attacttttc cttttttcat gtttgtttct tcctctcatt gttaatttta
    30961 gactgctgat tgttctaaag cttcgcggta agggatagaa ggttgcgcac cataagactg
    31021 cacagcaaca gaagaggcta gatttccata aaggatcgct tctttcaagt tactaaggtc
    31081 tggctgaagt gtgctgctta atgcgccaat aaatgtatca cctgcggctg ttgtgtcaac
    31141 ggcatccact ttaaaagcag ggacgatacc gtgttctttt tctgtatgat agaaagcgcc
    31201 ggcactgcct agtgtaatga ttactgttcc aatacctaat tcatggagtt tttcggctgc
    31261 tgcgaccaaa ctgttatgat ctgtcactct gattcctgta atgatttctg tttctgtttc
    31321 atttggtata atgatatctg tcttttcaag tagttcagta ggaatcgttt cacgagctgg
    31381 tgcaggattc aggatcgttg tttttcctgc gtcttttgca atagaaaaag ctaggatcgt
    31441 cgcatctaag ctactttcaa actgagcaat aataaagtca ctgtttgtaa tcagttgctt
    31501 gttttttagg acttcttgtt catggaaagc attatttgcc cctgcatgga tcaagatact
    31561 attttctcct gctgcgtcta ccataatcat tgcttgtccg gttgtggcgc cttggatttc
    31621 agctatccca gatgtatcga tgttttcttt tgtcagtaga tctaataact gttgtccttc
    31681 gctgtcagcc ccaacgcctc caataaaaga tgtccgtgcg cctgaacgtt tggctgccac
    31741 tgcttggttg gcacctttac ctccgccagc gtgaaaaatc tcatgcgcat gcatcgtttc
    31801 gcctggcttg ggcatgttgg ttaaacgaag ggtagtgtcc atattaatac ttccaattac
    31861 tgtaaccgtg ttcattttac aaacctccaa ctatctcatt tattttgatg ggaaaattat
    31921 tttttaaaac gttttagtaa aacgttttaa tagttcgata aaaatatagc acaccttgaa
    31981 agctatttca agggtgcagt tgaaaatctt ttttctaatc gtacaggtaa tcttttttct
    32041 tcccattctt tttctgggaa ttggatccga tctaacaata acttggcaga tgtttgtccc
    32101 aattcgaaga ttggttgtgc gatcgttgtc agtttcggct tgatatactc gcacatatct
    32161 atattatcat agccaataat gctgtagtct tcagggatgc tttttccagc ttcctctaat
    32221 ccgcgatata atccaaaagc taattcatca tttaaagcaa acacaccagt tgcagcagaa
    32281 tcaagaagtt ctgctgtgat ctggtagcca ccttgttttg agaactgtgt aggtaagagg
    32341 atcagttggt cgtgagggat ttggtaaaca tcaagagcag atttaaaccc ctcaatccga
    32401 gcgtggacat tttccggtgg attttcagga tagacaaggg cgatcgtttg atgtcctaga
    32461 ctaagcaagt gcatgcccgc taggtaacca ccgcgaaaat cgtctgttcg tacggcatca
    32521 ctaaatcctt cagatttttt ctgatccaaa acaatgaagg gccggccttg ttttttcaaa
    32581 ttttcgttga ttgcatctgt agagacagca gaagtcgcga taatgaatcc atccacacct
    32641 cgacgtgtga gctcggctag atattcgatt tctttttgat gatcagaatc agcattacat
    32701 aatatcgtga cgaaattctg cttatacaag atatcctcaa ttcctcgcat cagtgtactg
    32761 aagaatggat tcgtgatatc aggaacgagt acaccaattg ttttcgtttc cttggtaatc
    32821 atttgtctag caaaatagtc aggctgatag cctaattctt ctttggcagc taaaactttc
    32881 tgaacagttt tcggactgaa tttctccgta tttccattta atatcaagga gacagttgtg
    32941 attgaaacgc cggaaagttt agcgacttct ttgattgtga tctttttttt caccatgttc
    33001 atactcctat ctaaatcctg tggttatttt cccatgaaat caaaaagaag taaatgaaaa
    33061 tcacgtgtgc cagtacttat ttgctttgtt ggatagcttc gattatttgt ttcgttgagc
    33121 gtactcgtgc agttttcgga aatatctttt ttatgaacaa ttgatgggat tctttgtcac
    33181 gatcactgca agcatcttca gcaagcacga tatggtaccc gtgttgaaag gcatctaatg
    33241 cagtagcata tacaccgttt gttgtcgcta caccgcttaa aatgatagtt tcgattccac
    33301 gccggcgaag ttgaagatca agagaagtac caaaaaaagc acttggattg tattttgtga
    33361 tagtcaacat attatctgta tctttcaaag aatccttcaa aagtaaatca gtgaattctg
    33421 gaggtacaga agcagtcatt ccacccatat cagtttgttg actcaaataa gggaaagtgg
    33481 agatgtctac gttcactaaa actttcaagg cagcggtacg tttcaatgtt cttactaaca
    33541 aatcattgtt tgtcagaata gatgctgcag tgtgtggaga gagtgtaccg ttatttacga
    33601 tctctttttg catgtcgatc gcgacaaaag cagtttttga taaatcaaaa atcatatttt
    33661 cctttaaatc attcatgatg ttccttcttt caaataacaa tgttttttat gtaagtaaat
    33721 cataaatcgt ttatttgtca actaaattaa aggattcctt tctttagtct agagaaattc
    33781 agtggtaaaa taaataagtg tgagaaaacg cattcgatgc atagatatag cgagaaatat
    33841 cgtgaaaatg gaggaatcag agtgaagtat gtcgaagtaa aaaatgaata caaacgatac
    33901 aaaatgggag aaacgaccat tacagcaaat gacgatattt ctttttcaat tgaaaaaggc
    33961 gaattagtga ttatacttgg tccaagtgga gcaggaaagt caacgattct taatatttta
    34021 ggtgggatgg atacacctga tgaaggtcag atcattattg atgacacaga tattgcgcaa
    34081 ttttcagata aacagcttac tgcttaccga cgaacagatg tagggttcgt tttccaattc
    34141 tacaatctcg ttcctaatct aacagcaaaa gaaaatgtgg aattggcaac ggaagtttct
    34201 cctgatgcgc ttgatcctgt agaggtattg aggcaagtgg gcctagctca tcgattgaat
    34261 aactttccgt cccaattatc aggaggcgaa cagcagaggg tttcaattgc tcgtgcacta
    34321 gctaaaaatc ccaaacttct tttatgtgat gaaccgacag gcgcgctgga ttttgaaacg
    34381 ggcaagcaag tattgaaatt attgcaaaac gctagcagac aacacggcaa taccgttttg
    34441 atcattacac ataattctgc tttagcgccc atcgctgata gagtcattca cattaatgat
    34501 gctaaagtaa ggtctgttga attaaatgat catccttcat ccattgatga gattgtatgg
    34561 tagggggatt ttatgaaaac aagaacatat cttaaagcag gttttcgcga aattcggcaa
    34621 tctaaaggac gattcattgc aatcatcctt attgtcatgc taggaacact tttatttgta
    34681 ggtgtcaaaa ctgctggacc tgtcatgcaa aaaacgatgg atcactatgt tggtacagca
    34741 ggactttctg atttgcaaat cgtttctact ggtggcttga cacaaaaaga tattgctcaa
    34801 gcagaaaaga tcccagatgc aaaagtagaa acaggaaaac agatctatta ttccaatact
    34861 ggcaaaaacg aagtgattca aatattttct tataacaaaa ataacaagca gaacaagcta
    34921 caattaactg atggaaaact cccggaaaaa ccaaaccagc ttgtattgga tgaaaaagcg
    34981 gaagatgaag ggtataaaat cggtgatatt tatcagattg attcagatga attgaaagaa
    35041 aaagagtata ccatcacggg ctttgtccgt tctccattgt tcatcaacaa tttggagaga
    35101 ggatacgcga acgtcggaaa tggtagcgtc gattattttg tctatttacc tgaatccgtt
    35161 tttaaaacag atatttattc tgtcttatat cttgatttta caaacgtaaa gaatttgaat
    35221 acgtacagca aagcttacaa agataaaatg gagcaaaatc aagaaaaggc agaaaagtat
    35281 ttacaagatc gacccgaaga acgcttagaa gagctgaaaa aatcggccaa cgaagaatta
    35341 gaaccggcac agcaaaaaat agcggatggc aaagcacaaa caacgcaagc aagaacacgg
    35401 ttagaagatg ctaaaaaaca gttagcgcaa cagtcagcaa ctatccagca gcttcctgaa
    35461 gcgcaacgaa caatcgctgt tcaaacatta tctcaacaag aagctcaact tgctgaacaa
    35521 gaaaggcaat tagttgagaa agaacgtgaa ctggcctctg cagaggaaga attgaatcga
    35581 aatgcagaaa aaattgaaaa tttaactaaa ccatcatacc tatacaatga acgttcagaa
    35641 aatgtcggtt ttcaagaatt tggtgatctg tctgatcgta tcgctgcgat tgccaatgtt
    35701 tttcctgttt tctttttctt tattgccgca ttgatcacct ttacgacaat gacacgtatg
    35761 gtagaagaaa accgcaggga aatcgggaca ttaaaggcgt taggttatac gaaagttgaa
    35821 atcgcaaaaa aatatgcaat ctatgcaagt ctagcctcag ggataggtat tgttttagga
    35881 acgatattag gaacgaatct tttgccaagg atcatttttg agctgtctaa cgagcggtat
    35941 gatattggga gtgcgcttat tttttatgac tggccgccga ttttacaagc agctgccgca
    36001 ttcttcattg ccgcatttgg cgctgcaatg atcgttttat tcaaagattt aagagaacgg
    36061 cctgctgcat tgcttcaacc gaaagcaccg aagccaggga aacgcatttt tcttgaatac
    36121 atcactccgc tgtggtcacg ccttagcttt aatcaaaaaa tcagttaccg aaatctcttc
    36181 cgatataagt caagaatgct tatggcgatt atcggaattg ctggatgtgc cgggctgatg
    36241 gttgcaggcg tgggattgaa agattctcta agttctgttt cagccaaaca atttggaccg
    36301 atcattgact atcaagcaat cgttacattg gattcatcca cggaagatag tcgtcaaaaa
    36361 gcagcggata tgctgcagaa gcaagaaaaa gtaaatgaga tcctagctat tcatactcaa
    36421 accgtagaat tgagaaaaaa aggacatgct tcgcaaagtc tgacagtgat cgtaccaagt
    36481 aaaacagctg atttggatcg ttttatccat ttaactgatc ttgaagagaa cccacgctcc
    36541 ttgcctaaaa caggaatcgc tattacgcaa aaagtagcag aactatttga cttatctaca
    36601 ggagatcata tttcgcttta tgatgaagat caaaaagaat ggaaagtcga agtaaaagaa
    36661 atcgtgaaaa actatctagg tagctttatt tatatgtccc ctgaatatta tgcttcagtt
    36721 actgatcaat cgatgatgga taatgcattt ttgctgcgta cagataagat gacaacaaaa
    36781 gaagaagaaa tgctttccga gaaactgctt gccacagacg ctgtgacaaa tacgtctttc
    36841 atgtccaaac aaatcgaaac gcaagaagaa tcaatggcga acttagactc ggttgtctta
    36901 atctttgtcg tattatcagg actgctggct tttatcgtct tgtataatct aactaatatc
    36961 aatatttccg agcgtgtacg cgaattatct acaataaaag tccttggatt ttttgataaa
    37021 gaagtcacga tgtacattgt tcgagaaaat atcatcttta ctcttttagg aatcatcgga
    37081 ggatttggtg taggatatat attgactgac ttcatcttgc aacaagcgtc tatggagaat
    37141 gtcatctttc cattagtgat cacatggtgg gcttatgtct tatcagcagg attaactatc
    37201 gtatttacgg ttattgtgat gattgttacc catttcaaat taaagcatat cgatatgatt
    37261 gatgcgttga aatcgaatga ataaaaacag aggttgtgaa atcagcggtt tgacctgagt
    37321 attagagaaa caaacggcaa aatagttttt catattttgc cgttttttga gctaataccg
    37381 aaggacagct gattgaacac catgaatcag aggttgtaaa aaagccgtcc tgcatcgagc
    37441 aaaaagaata ctcaaataaa attagctcct catgttttac atttgagaag actgattgcc
    37501 gtagataaaa gcttttgaac accatttata gaagaaacaa gaatattttg ctattttaaa
    37561 caagaggata gacaaataca tgtctgatct cttgtttttt attgggcaac agacctctta
    37621 gaatgtataa agataaattt tacaaacgct ttcggttcag ttataataat aaatgataac
    37681 ttgtatatat aagttgtggt aggtaataaa tcagttgaat ggaggtaaat atccatgaca
    37741 tttaaagaca aagtcattta ccaaatctat cccaaatctt acaaagacag taatggtgat
    37801 ggaattggag atttaaaagg aatcaaagaa aaactcccgt atctgaatga tctaggcatc
    37861 gacatgattt ggctaaaccc tatttatcca agccctcaaa aagacaacgg ttatgatatc
    37921 tctgattaca cagccatcga tcctatcttt ggtacgatgg aggaattcga acagctaata
    37981 gccgaagcaa agaaataccg gatagaaatc atgctcgata tggtgctgaa tcacgtttcg
    38041 acagaacatg aatggttcaa aaaagcacta gcaggggacc agcattatca ggacttcttc
    38101 attttaagag aagagccaac ggattgggta tcaaaatttg gtggaagtgc ttggtcgcca
    38161 tttggagata cggggaaata ttatttgcac ttatatgatc ggacacaagc tgacttgaac
    38221 tggcggaacc aagcagtaag agaggaacta ttcaaagttg ttcgtttttg gatggaaaaa
    38281 ggggtaaaag gattccgttt cgatgttatc aatgttatcg gtaaagacga acaattgaaa
    38341 aataataaag aaaatgatgg caaacctgaa tatacggaca aaccaatcac ccatacgtat
    38401 ctaaaagaat tgaaccaagc aacgtttggg acggattcca ctatcatcac agtcggtgaa
    38461 atgagctcta cgacgatcga aaattgtatc ttgtatacag aacctagtag agaagaatta
    38521 tcaatggtct ttaatttcca tcatttgaaa gtcgattatg aaaatgggaa taaatggtct
    38581 actcctccat ttgattttgc agaattaaag cgactatttc atacatgggg agaaaagatg
    38641 agtgagggta acggctggaa tgctctattt tttaataatc atgatcagcc acgtgcgctc
    38701 aatcgatttg tagctatcga tcagtatcga aaacaaggag cagagatgct agcaaccatg
    38761 atccatttga accgtgggac gccctatatt tatatgggag aagaaatcgg catgatcgac
    38821 ccagactatt catcgatttc cgactatatt gacgtagaaa gtttgaatgc ttataagtta
    38881 ctccttgaac aaggattcac agaaaaagaa gcgtttcgtc ggatcaaagc aaaatctcgc
    38941 gataactctc gaacacccat gcaatggtca gacacagaac aagcaggatt tactacaggg
    39001 aaaccttggt taaaagttgg aggcaagcta gaagagatta atgtagaaaa agaactatct
    39061 tctgaagatt ctattttgtc ctattacaag aaattgattc ggctgagaaa aacatatcca
    39121 atcgttgcac aaggagatta tcatgcttat gaagcaaatc atccggaagt ttatggcttt
    39181 cttcgccaat ttgaaggaca gcagctgctt gtattaacaa atttctatga taaagataca
    39241 aggatcgcca tcccagaagg attcacagaa gccaatattc ttatcgataa ttattccgat
    39301 actgttcttc atcaaagtct ggagctaaaa ccataccagg caattgcttt gctgaaaaat
    39361 taaaacaaaa acacaaaacg tcgttcctac gagctattct cgtaagatcg acgttttgtg
    39421 ttttatttaa aataatcttc ttcagaaaat gaagagtttt catactctcc tttatcgcca
    39481 taaaatttat tgtagcgttg tttgaattta cggaaaataa aagtaacgag taatttgact
    39541 aacgtataga taggaatacc aaaaataacg cccacaattc ccattagatc acccatgaca
    39601 agtaaaacga ttaagatcgt aatcggatgg atctgtaaac gatcacccat tactcgagga
    39661 atcaccaagt caccgtggag aagttgtacg gcaaaccaga caatgacaaa tttgacagcc
    39721 ataaaagtag agtcttggaa tgcaatgatc aaacctggaa agaaggcgat aaacggccca
    39781 atatatggaa tgatacataa caacccagca gaaatagcta aaacactagc aaattctaat
    39841 ccgatcagta agaaacaaac ccagtagact gctccaagga taaatgaagc aatgatttgc
    39901 ccctttagaa atgaacccaa ttgttgatta gcaatctttg tcaaattttt aaaatctgta
    39961 cgaaatgctg gtgggacaat cgctaaaact gactgataaa atttttgagg atctctcaaa
    40021 aggaaaaaag cgacaattgg accagtaaat aaagtaataa aaatagctgt aacagttgaa
    40081 aaaatgttgc tgatccgttg tgcgccactt tcccaataat ttccaataaa tccagctata
    40141 tcagacgtca cgtcgtcaat agaagcgaaa aattgattaa atgaatcggc aaaaggtgtt
    40201 ttctccaaaa attgtcggag actctcttga aaatcttgaa acaaatctgg caaactactg
    40261 atcaatgtct ttgcctgttc ttcaagaaaa ggaataagcc atatgcttcc aagccccaat
    40321 aaaaataaaa taatcacata aacgataatg actgacaagt tcctcgaaag gtgcttgctc
    40381 aaacgtttga tgactggttc caaaagataa tacaagatca gtgcaaacaa taatgggggc
    40441 aagatagtag cgataatgat tccgacaggc ttgaatataa acgagatctg atggaaaaaa
    40501 taaatgatca gcgcaagtaa gataactatt cctaaaacat agtaagaggt ttttcctcct
    40561 aaaaagcgga tcaaacgatt cgttgtatct gtttcttttt tcactctttt tcctccttta
    40621 tctataactt cattatagac aaaagcgggt ataaaaggaa aatgatagag gttgattgca
    40681 aaaatatcgt caatcattca tataaacttt ctcatcaatt aaaataagaa aacgcttgac
    40741 agttctttta accatatgat aaattaaatc ctgttgctaa aacgtaacta ttacggtttg
    40801 cgaaaagcga atcggaacat tcaaatgaaa gaagatgaga agaggagtgt cataaatgaa
    40861 acgattgata ggtatattgg ccatgctggt tttagcaggt atgttaactg cttgcggggc
    40921 aagtgggaaa gccgaggaca gtaaagaaaa attgagcgtg atgacgacct tttatcccat
    40981 gtatgatttt acaaaagcaa tcgtcgggga tgaaggagaa gtagagttat tgattccagc
    41041 agggactgat tcccacgatt atgaaccttc tgctaaagac atggcaaaga tccaagatac
    41101 agatatcttt gtttataatg atgaaaacat ggaaacatgg gtccccgcaa tccaaaaaac
    41161 attacaagaa ggcaatgtcc atacgataaa agcgacagaa ggtatgcttt tacttccggg
    41221 aagtgaagaa ggacatgatc atgaccatga acacggggaa gaaggccata cacatgagct
    41281 agacccacat gtatggctag ctccaagttt agcaatcaag caggtagcaa acatacgtga
    41341 tcagctgatt gaagcttacc cagaaaaaca ggaagtatgg acaaaaaatg cagcagccta
    41401 taccgagaaa cttcaagcac tccatcagct ttatcaagaa acctttaagc aagcaaaaca
    41461 aagatctttt gtcacgcaac atgctgcatt caactatcta gcgttagaat atggattgaa
    41521 tcaagtatcg attgctggtc tttcttctag tgaagaaccg tctgcggcga gaattgctga
    41581 actgaaacac tttgttaaag aacatggaat caactatatt tattttgaag aaaacgccaa
    41641 agatagtatt gccagaacgt tagcaaacga agcaggtgta tctttagaag tattgaatcc
    41701 tttggaagga ttgacgaatg aacaaataga aaatggagaa aactatcttt ctatcatgga
    41761 agcaaacctc gaagcgttga aaaaaacgac agagacagaa aacccgctag aagatacttt
    41821 gacccccaaa aaagaaaaga gcgtctacaa cggttatttt gaagacgagg atgtacaaga
    41881 tcgctctctt tcagattggg caggaacttg gcaaacagta gatacgtatg tagaagatgg
    41941 gacttttgat cctgtttttg aatataaaga aaaattgaat caggataaga cagcacaaga
    42001 atataaagaa tattatacga agggctatca aacagatatt gcttcaatca agatcagtga
    42061 gaaagagatg gcatttacgt ttaagaatgg aacaacgaaa cgttcaaatt atcgttatgt
    42121 agggaaagaa atcttgacct acgaggcagg aaaccgtggc gtaaggtatc tattcgaagc
    42181 agaagataag gaaagtggcg cttttagata tattcaattc agtgatcaca cgattgcacc
    42241 ggctgactcc gaacattttc atttatatgc aggaaatgaa agtcaagaag tattgctgaa
    42301 agaaatggag aattggccaa cattttatcc agatacaatg gatggacgtg aaattgcaca
    42361 ggaaatgctt ttgcactgat aggcaaaaaa gaaccgaagg gtttggatcg gctggtcatt
    42421 tagtcggtcc aaactattcg gttcttttta tgaaatcaat ttctttaatc cacggataga
    42481 ggctgggatg gtctcgccgt ttgccaacgt gacttctctg gtttttttat taatcgaccg
    42541 cacattttgt gtattgataa cataggattt atgacaacgg ataaaattcc gatgcatttt
    42601 ctcgatttct ttgatcttct catagaattg taattgggca tgagtcaaat ggacttccag
    42661 tttatgtggc gctcctagcg aagtagaaaa atattggatg tctttgatcg gtaaaaattt
    42721 gatctcatca ttaaactcta ttttgaagat atctttttgg atttgggaca tttgaaaatc
    42781 ttcgaaaatt ttgctcagga tatgctcgac attttctttt aaatcataaa gatcttcttt
    42841 gacgatataa tcagtcggtt cgatgttgct ttttaagatt ttcatcgaca tttccgtatg
    42901 ggaagtcaca tagattattt ttcctaaagg atctaattca cggatcaatt taccgatcgc
    42961 tacaccatcc atttcactgt cttctagttc gatatctaaa aagtaaatac caaaagaaga
    43021 agttctgatc ttggctgcat tgagaagttc ttccggatca gaggtagaaa tatggacata
    43081 actgtctaag ttttcaatca tgattctatt tttgatcacc gttgataaaa cttctaattg
    43141 ttttggatcg tcttcacaaa tataaatggg aatcatatca ttactccttt attttatttg
    43201 tatataaatc gtttgagaaa aacgctggtt ttcaattgat gtttcaagga aaagataagg
    43261 attggtcttt gtcaattcat ccaagataaa caagccaagg ccttcgtttt ttggatttgt
    43321 ttttgttgag aacccttttt gtttcaactg gtttaacggt aatgtatttg ttgttgtatt
    43381 gatcacacga atcatcacgg aatgattctt cttgagtatg gtcaattcga tttttttctt
    43441 ctttgcagca ctagctcctt caatcgcatt gtccaagata ataccaagca tacgtacaag
    43501 gtttaccgta tgtttcgtat ccagttcgat tggctcagga aaatgaagct ggacgatcaa
    43561 ccctttttcc tgtgccatca tgagttttaa tgacagtaaa ctacggatct caggaacttt
    43621 catattatcc aacatactta gattggccgg aaaaaggttc atcatctggc gtgtaggtaa
    43681 tatcgtttga tcataataag accgtaatcc ttctaagtcg ccttctgaaa tatagctgtg
    43741 aagagaaaaa agcagatttt ggtaatcatg tttgaattct ctcaagtctt gatagttttt
    43801 ttctagatct ttgatataat cctgttgcat cctgacttgt tgctctaata actgatcagc
    43861 taatttctta ttagagattt tccaataata aaccaagatg atcaggccaa cacacagata
    43921 tagaatgaaa aaactcgtat tcaaagctaa gacatcaggt gtttcaccag aaaaacgggt
    43981 ataaaaaata atgatatagt atgtaaataa tatcaatgtc ccgatcgtac cgatcatacg
    44041 gcggtccaca gtgtgaaacc aagtctctaa cgattttttt aaacaaaaag ctaataaagc
    44101 agataaaaaa gcggataaga caatgtgaaa atactgcaat gaatctcctg gttcaataac
    44161 cgaagatttc aaaacaaaaa aatcgccgat gcttgttaag tgatctccta aaagcgacgt
    44221 caataaccca aaaggaagcg cgtaaaccaa ttgttgacgc gaagtcgaaa agaagaaaaa
    44281 agtaagcatc agcattccta tagcaattag agcagtaaaa atccctaaaa tcgaatagac
    44341 ccatccaatc aaaatacaag aaatcagaat gactcccgca aacagatatt tgtatcggat
    44401 gtgatggtca atcaataaga tacaaattgt gtaaatcaaa tattgcaacg ctatggacca
    44461 taaaaacata aagtacagct cccattgatt tttaccaaat tatagcacaa ttggcgtatt
    44521 atttttttaa tttttcaggg attttgaatt cagaactcat accaaagcag cagccagata
    44581 caaaaaagtc agataagtta gttaattgtt taatgattgt ttttttcatg aaagattcct
    44641 ccaaaattca tcaagtagtt tgtaatagga agcaacgtag ctatttctaa aacaatccca
    44701 agtagcaaat aggtagaaaa gtaagaagct gagaaaaata acgtaatgag agaaaaaatt
    44761 agaaacatgc tgatcgcctt ctttaataat gggggacgtt tctcgggttc aatgggattg
    44821 attgctgtac ctcttgatcc atagcgaagc agtaacaaaa agcagctgat taacaaaaag
    44881 accatcattg ggaaggagat agtcaattgc agatgtttca tcaccaaggg aacgccccat
    44941 aaataaagta gtgtccacag cagacaaacg aattcagagt cggaatgcaa tccataagca
    45001 tatctgcgga tgcagatata agaaagaaag gtgattcctg tttccgccca tgagcctgtc
    45061 accagagaaa agactagaac gaccgctagt tttccaagat tattcaataa aacttctaag
    45121 acaaatttta cttggacata caccatatct tcttccgatt tgtctccgct gaataaggct
    45181 tctaataatt tgttcacaac tttatctttc atctcatacg tacctccacc acaaatttta
    45241 tgctaaaaaa aataaaaaac gtcttttgtt tcataaaaag tggttttgac atcaaaaccg
    45301 tcatgatctc ctaaaaaaat cttcgttttc caagaacctt accaaaaaaa gtgacagttt
    45361 tgatacgaaa agtacttttt atgacaagtt tgtgcttttt cgagtgattt atgttaaaaa
    45421 attactgacc ttataaaagg tgatggaggg attcttatgg aaaatgaaca attgtttgaa
    45481 gtcggcgaaa catatgcatt taaatctgat ttatttgaat caccaataaa aggaaaaata
    45541 gaaaaaatct atgaaaatag tgtgatgata cgtgtcatga aatgcgcagt agaagacaat
    45601 gataccgctc ataatctgca ctggcagtta gttgtacgca aatcaagtac aatagaagaa
    45661 taaaaacaga taaaatgttg agagcataaa gaggttgtga gtctcccgtt tagctctgag
    45721 ttacaaggaa taatgttaaa aaacagcttt tcatgtttta tgaatattgc cttgtgaccg
    45781 aggtgttggc aggagaatac tgtttatttg aaaaaagggc tgcgacaaga cttttgtcac
    45841 agcctttatt ttcatgaata ggagttactt ttggctactt ttagagaagt agcttttaag
    45901 gagcagataa agtcaaggct tttatttcta taaatgaagt agtaaaatga agaacagatt
    45961 ttattacagg aaggatgccg ataattgtga tcattgttgc aggaacgatc ggtgcaggga
    46021 aaagcacact aactgaaatg cttgcccaag atttagaaac aaaacctttt tatgagaacg
    46081 tggaagataa tgaagtattg ccgctatttt attctaatcc agaaaagtat acttttttat
    46141 tacagatttt ctttttgaac aaacgctttt tggcaatcaa agatgctttc agccatgatg
    46201 acaacgtact agaccgctcc atctacgaag atagtatgct gtttcatttg aatgcagatt
    46261 tagggcgtgt ttctgaagta gaagtaaagc agtatgaagg tcttttagaa acgatgctga
    46321 aggagttaga agaaatttcg ccacaaaaga aaccagatct actggtttat attcgtgtat
    46381 cctttgaaac tatgttagcg agaatcaaaa aaagaggccg ggaatatgaa cagctagaac
    46441 aagatcctga actctattcc tactataaag aactcaatcg acgatacgaa gagtggtatg
    46501 aacagtttga tatatgtccc aaaatagtga tcgatgggga caaatatgat ttcgtagccg
    46561 atccggcatg tggagaacaa atcgtacaag aaatcaagat gcgggcaaag aaaatgacag
    46621 aagaggcaga tgcccagtcg atccgctaca ctacaaataa cccgaaagca aagggaatct
    46681 ttacaactaa ataatataaa aactaagcaa attgctcact cgcagatatt tctctgtgag
    46741 cagcatcttg cttagttttt ttgattcatt gagatcaaat agtgaaccga ttcgtatgat
    46801 aaaattgcca taaatgaaat gtctgagtga gggccatgat caaaaataaa atggaaacga
    46861 tcaaaatcga atgggcgcca aacagatgat gacagaaaac aaggacaata gctcctgcga
    46921 taaaggcaag aacgacactt agatagtgga aaaatcgatc aaaatctgct ttttctttag
    46981 aaatacaata atcatataag ttcgttgcta ccccttttaa atttccagtt gtaaataaat
    47041 tagtgtaggc acgcccgttg attttatcaa aagctaccca ctggatcgat gcagtaaaag
    47101 caatcaaaat cgttactaat gcgggatgct catggacggg tgtcaaacta acaaagaaaa
    47161 aaatcactgc ttcataatat agaacaaaca agcgccagaa atgtttttct ttttttctaa
    47221 aataatcaat tagatacttt gttagaagaa tccctaagaa aaagaaaagg gtagacaata
    47281 cttttttacc aacacttagc cattctccgt caaatgcttg gatgatcgat agaatgatat
    47341 tgccagtttg agcagaggca aaagcaccat attggatata agtataactg tccatggctc
    47401 ctccgataaa tgtcagcaaa agtcctattg tacgcccctc gtgaaaggaa gtctgcattt
    47461 ttttcatcac cttttatgaa tatttttgtc ttccttatct tagcataaac gattgaaaaa
    47521 aaaggaaaaa ttttcagaaa actaaaatca tatcgcttcc cattttcatt tttagaaagg
    47581 tctgttagta taaagaaaaa atagacaacg aaactttaaa taaccaactt gaaataaaca
    47641 aattcaaaga agaaagatgg gaaggaaatc aatcagttga atgaaaaaga aattctccga
    47701 ttacaaaaag aattgaaacg ctgggagaac aagtataaaa agcttcaaca agtctttcgg
    47761 ctacaagaag aaatcattgc tacctatgaa gcggcttatg gcgaagcacc cggacttatt
    47821 gaggaagaag tacaagtaaa agaaagtgaa ggaaacagct ctttctttaa aatgatggag
    47881 aaacgttcct ataatcgctt caatcaagaa caaaaagcag ctatcacata tgatatgaag
    47941 aaacatcttc gaatcatagc aggagctggg agcggcaaaa cgcaaacaat ctgtgcaaaa
    48001 gctgtttatc tgatgacgca aaaacaagta gacgaagaac gcatactaat gatcactttt
    48061 acaaggaacg ctgcaaatga actaaaaaaa cgagtagata atttcagcca gagaaaaacc
    48121 gctgtccata tcggaacatt ccatagtatc ttttttagga tatacaatga aatttgtcga
    48181 aaatttcctg aagtagcaat gcaaggaata caaggagact tttcaaagga tactgcacaa
    48241 aaagtgaatg ctgttttgca acagctgata cgtaaatata atctttatat atttgatcaa
    48301 tatggagaaa aaacgattgc ttctcggtta gactattggc agaatatgaa cctttcagta
    48361 gaagaaatgg ttcagttagt taaagaaaaa tatgattcca ttgataagaa tgcacgacag
    48421 ccgatatcag aacgactgta tggattgctc acggaactcc aagaacaaaa aagaagccag
    48481 caattgctag agttcaatga tgtactccaa aatctgaaaa gcgctttgga gaatgaggag
    48541 atccagcatt atatcggcca aaaatatgat tatctattca ttgatgaatt tcaagatact
    48601 aatccacttc aatgggaaat cgtgaaacgg atgacaaagg aaaacaggat caagttgatt
    48661 gttgttggag atgatgatca gagtatctat ggattcagag gatcagagcc agcatacatc
    48721 aagaactttg aaaaagaata tcctaccaaa actcttttct tattaactaa ttatcgttct
    48781 cgggcagcta ttgtccaagg agcaaataga ttgatcagct ataataaaaa tgatcggata
    48841 ccaaaggcga tgattccagc acaaaaagaa gcaggtgtta tggaagccta tttgttttct
    48901 gatactaaag cagaaagtca gtggctaatc aaacaaattc agacatttat tcaaaaaaat
    48961 gggaaatata aggaatcaat catattatac cgatcagctt cacaaacgca gcagcttgtc
    49021 caatctctgc tgaaaacgaa tattcctttt gtactggaag cagatagtcc atatgaaggt
    49081 atctttggta tcaagtcttt ccaacatttc tatcaaaaaa ttgttcattg gcaaacagca
    49141 ctgaatctgc aaaagaaaaa tcaggcctac cagcaattgc tccggcagct gatggcagac
    49201 tgctacctga aaaaaagtga atgcgatcaa tacttttctc ctaaatatca aaatatagcc
    49261 gttgcagatt atattttgtc gataagcccc aatttaaaat cgaaatctgc agaaatccga
    49321 gagtttgagg aagcgttgcg taaaacagac cagcatcaag aaatctattc gcttgttcaa
    49381 agctatcttc atctgccacg tatagctaag gaaatcgacc agagtgatca agaatggata
    49441 cgagaagaag taaagcagca tgctactttc agaagactcc aagccttatc gcaagaaacc
    49501 caagcgacag ctaaagaatt aaaaacccgc ttgattgctt acagacaagg aaaacttgat
    49561 gcactatgta tccagagcat tcacaaaagt aaaggtctta gttaccgaaa tgtctttttg
    49621 attggttgta acgaaggaag tttgccttat aacggagcga cagaaaagaa agtaatcgac
    49681 tttaatgaaa taaaagcaga accagtaacg acgatagaag aagaacgaag actattttat
    49741 gttgcgatga ctagagcaag aaatcgtctg tatctatgtg tacctcaaat gaaaaattca
    49801 agaaaattga aagtatcgag atttatcaaa gaaaccggga gtcgtgtgaa aaaaggaaag
    49861 tgatagaaga gagggtgtga cagaagtggt cagtttcaag aaagaagaag gaatgtacga
    49921 aaattgcttc tcaaattttt gtgaaatttc agcttatttt cgaagaagct atttctggaa
    49981 cacagtttat tcggaaaagg gaggtgtgac aagaattttg tcacacctcc cttatttatt
    50041 ttgaaaatga
//
LOCUS       DAYYGO010000006        43366 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000006 DAYYGO010000000
VERSION     DAYYGO010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.006"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            118..972
                     /locus_tag="ACHA5T_000309"
     CDS             118..972
                     /locus_tag="ACHA5T_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFM8885917.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            973..2310
                     /gene="xseA"
                     /locus_tag="ACHA5T_000310"
     CDS             973..2310
                     /gene="xseA"
                     /locus_tag="ACHA5T_000310"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFM8885918.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            2315..2539
                     /locus_tag="ACHA5T_000311"
     CDS             2315..2539
                     /locus_tag="ACHA5T_000311"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFM8885919.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            2541..3431
                     /locus_tag="ACHA5T_000312"
     CDS             2541..3431
                     /locus_tag="ACHA5T_000312"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFM8885920.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            3440..4258
                     /locus_tag="ACHA5T_000313"
     CDS             3440..4258
                     /locus_tag="ACHA5T_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFM8885921.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            4365..4814
                     /locus_tag="ACHA5T_000314"
     CDS             4365..4814
                     /locus_tag="ACHA5T_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM8885922.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            4832..6514
                     /gene="recN"
                     /locus_tag="ACHA5T_000315"
     CDS             4832..6514
                     /gene="recN"
                     /locus_tag="ACHA5T_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFM8885923.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(6569..6685)
                     /locus_tag="ACHA5T_000316"
     CDS             complement(6569..6685)
                     /locus_tag="ACHA5T_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFM8885924.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(6758..7714)
                     /locus_tag="ACHA5T_000317"
     CDS             complement(6758..7714)
                     /locus_tag="ACHA5T_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM8885925.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            7885..8253
                     /locus_tag="ACHA5T_000318"
     CDS             7885..8253
                     /locus_tag="ACHA5T_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFM8885926.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            8423..8854
                     /gene="mraZ"
                     /locus_tag="ACHA5T_000319"
     CDS             8423..8854
                     /gene="mraZ"
                     /locus_tag="ACHA5T_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFM8885927.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            8875..9834
                     /gene="rsmH"
                     /locus_tag="ACHA5T_000320"
     CDS             8875..9834
                     /gene="rsmH"
                     /locus_tag="ACHA5T_000320"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFM8885928.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            9839..10240
                     /gene="ftsL"
                     /locus_tag="ACHA5T_000321"
     CDS             9839..10240
                     /gene="ftsL"
                     /locus_tag="ACHA5T_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFM8885929.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            10241..12433
                     /locus_tag="ACHA5T_000322"
     CDS             10241..12433
                     /locus_tag="ACHA5T_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFM8885930.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            12459..13421
                     /gene="mraY"
                     /locus_tag="ACHA5T_000323"
     CDS             12459..13421
                     /gene="mraY"
                     /locus_tag="ACHA5T_000323"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFM8885931.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            13435..14796
                     /gene="murD"
                     /locus_tag="ACHA5T_000324"
     CDS             13435..14796
                     /gene="murD"
                     /locus_tag="ACHA5T_000324"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFM8885932.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            14822..15910
                     /gene="murG"
                     /locus_tag="ACHA5T_000325"
     CDS             14822..15910
                     /gene="murG"
                     /locus_tag="ACHA5T_000325"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFM8885933.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            15925..17145
                     /locus_tag="ACHA5T_000326"
     CDS             15925..17145
                     /locus_tag="ACHA5T_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFM8885934.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            17301..18626
                     /gene="ftsA"
                     /locus_tag="ACHA5T_000327"
     CDS             17301..18626
                     /gene="ftsA"
                     /locus_tag="ACHA5T_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFM8885935.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            18649..19890
                     /gene="ftsZ"
                     /locus_tag="ACHA5T_000328"
     CDS             18649..19890
                     /gene="ftsZ"
                     /locus_tag="ACHA5T_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFM8885936.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            19907..20584
                     /locus_tag="ACHA5T_000329"
     CDS             19907..20584
                     /locus_tag="ACHA5T_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFM8885937.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            20598..21197
                     /locus_tag="ACHA5T_000330"
     CDS             20598..21197
                     /locus_tag="ACHA5T_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFM8885938.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            21278..21499
                     /locus_tag="ACHA5T_000331"
     CDS             21278..21499
                     /locus_tag="ACHA5T_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFM8885939.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            21548..22330
                     /locus_tag="ACHA5T_000332"
     CDS             21548..22330
                     /locus_tag="ACHA5T_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFM8885940.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            22351..23058
                     /locus_tag="ACHA5T_000333"
     CDS             22351..23058
                     /locus_tag="ACHA5T_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFM8885941.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            23320..26118
                     /gene="ileS"
                     /locus_tag="ACHA5T_000334"
     CDS             23320..26118
                     /gene="ileS"
                     /locus_tag="ACHA5T_000334"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFM8885942.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(26167..27693)
                     /gene="zwf"
                     /locus_tag="ACHA5T_000335"
     CDS             complement(26167..27693)
                     /gene="zwf"
                     /locus_tag="ACHA5T_000335"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFM8885943.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(27708..28355)
                     /locus_tag="ACHA5T_000336"
     CDS             complement(27708..28355)
                     /locus_tag="ACHA5T_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFM8885944.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(28539..28868)
                     /locus_tag="ACHA5T_000337"
     CDS             complement(28539..28868)
                     /locus_tag="ACHA5T_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFM8885945.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            29045..29773
                     /gene="sfsA"
                     /locus_tag="ACHA5T_000338"
     CDS             29045..29773
                     /gene="sfsA"
                     /locus_tag="ACHA5T_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFM8885946.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            29789..30802
                     /locus_tag="ACHA5T_000339"
     CDS             29789..30802
                     /locus_tag="ACHA5T_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8885947.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            30802..32079
                     /locus_tag="ACHA5T_000340"
     CDS             30802..32079
                     /locus_tag="ACHA5T_000340"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM8885948.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            32142..34844
                     /locus_tag="ACHA5T_000341"
     CDS             32142..34844
                     /locus_tag="ACHA5T_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8885949.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            34996..35313
                     /locus_tag="ACHA5T_000342"
     CDS             34996..35313
                     /locus_tag="ACHA5T_000342"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8885950.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            35343..35663
                     /locus_tag="ACHA5T_000343"
     CDS             35343..35663
                     /locus_tag="ACHA5T_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM8885951.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            35771..37231
                     /locus_tag="ACHA5T_000344"
     CDS             35771..37231
                     /locus_tag="ACHA5T_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8885952.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            37299..37520
                     /locus_tag="ACHA5T_000345"
     CDS             37299..37520
                     /locus_tag="ACHA5T_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8885953.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            37551..37733
                     /locus_tag="ACHA5T_000346"
     CDS             37551..37733
                     /locus_tag="ACHA5T_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFM8885954.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            37733..38146
                     /locus_tag="ACHA5T_000347"
     CDS             37733..38146
                     /locus_tag="ACHA5T_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFM8885955.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            38269..39450
                     /locus_tag="ACHA5T_000348"
     CDS             38269..39450
                     /locus_tag="ACHA5T_000348"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8885956.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(39521..39685)
                     /locus_tag="ACHA5T_000349"
     CDS             complement(39521..39685)
                     /locus_tag="ACHA5T_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8885957.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            39839..39912
                     /locus_tag="ACHA5T_000350"
     tRNA            39839..39912
                     /locus_tag="ACHA5T_000350"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39873..39875,aa:Arg,seq:cct)
     gene            complement(39981..41120)
                     /locus_tag="ACHA5T_000351"
     CDS             complement(39981..41120)
                     /locus_tag="ACHA5T_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8885958.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            41292..41426
                     /locus_tag="ACHA5T_000352"
     CDS             41292..41426
                     /locus_tag="ACHA5T_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8885959.1"
                     /translation="MIKVIFKRITELPEFRLFVDYWYVWFFIVALLIGLDVILERKLK
                     "
     gene            complement(41419..42054)
                     /locus_tag="ACHA5T_000353"
     CDS             complement(41419..42054)
                     /locus_tag="ACHA5T_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8885960.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(42167..42802)
                     /locus_tag="ACHA5T_000354"
     CDS             complement(42167..42802)
                     /locus_tag="ACHA5T_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HFM8885961.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(42836..43297)
                     /locus_tag="ACHA5T_000355"
     CDS             complement(42836..43297)
                     /locus_tag="ACHA5T_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HFM8885962.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
BASE COUNT        14341 a         7376 c         9076 g        12573 t
ORIGIN      
        1 gaattagaag ctggggcgta agtcctagct tttttctatg aatcatcata aacctgttat
       61 aaatcgggaa aattctatgg tagaatgaaa acacaaaaaa tatctgggag tggttagatg
      121 gcagaattga ttaacggaaa agaattagct gaaaaaatgc aggcagagat tgctgaaaaa
      181 atccaaaaat tgaaagataa tggtatccat cccggattag tcgttttatt ggtaggggaa
      241 aatccagcca gccaaatcta tgtaagaaat aaagaacgtg tagcaaaaga aatcggaatc
      301 cattcattgg tagagcgtta tcctgagacg atttctgaag acgaactact tgcagaaatt
      361 gaaaaataca atcaagatcc tagttttcat ggtatattgg tccaattacc attgccaaag
      421 catatagatg aagaaaagat cctgttagct atccgccctg aaaaagacgt agacggtttt
      481 catccgatga atttaggcag actccttgcg ggtaaaccgg acaagattcc ttgtactcca
      541 tacggcatca tgaaaatgtt tgaagcttat tctatcgatc cggcgggtaa acgagcagta
      601 gttatcggac gaagcaatat cgtcggtaaa ccgatggctc agctactctt gatggcagat
      661 gcaactgtta ctgttgctca ttctaaaacc gaaaatctgg ctgaactggc acaagaagca
      721 gatattttag tcgtagcaat aggacgtgga cattttgtca caaaagaatt cgtaaaacca
      781 ggtgctgtag tgatagatgt agggatgaat cgtgatgcta acggtaagct gatcggtgat
      841 gtgaaatttg atgaagtgga gcctattgca ggctggatca ctcctgtgcc aaagggtgta
      901 gggccaatga ctatcacgat gctgatgtat caaaccgtaa aatctgccga agcttttgga
      961 gcaggtgaat agatggcaca agaatattta actgtcacta cattaacgaa gtatttgaaa
     1021 agaaaatttg atgccgatcc ttatttagaa cgcgtatatt taacaggcga aatatcgaat
     1081 ttccgtttac gtgccaatgc ccatcaatat ttcagcttaa aagatgacca tgcaaaaata
     1141 tctgctatta tgttcaaagg agcattccaa aaattacgat tccagccaaa agaaggaatg
     1201 aaagtccttg tcgttgggcg tatttcatta tatgaagcgg gaggttctta tcaaatctat
     1261 gtagagcaca tggagcctga tggaataggt gctttatatc aagaattgga agaacgaaaa
     1321 gaaaagctga tgaaggaagg cctttttcaa gggcctaaaa aaatgctgcc acgtttccca
     1381 aagcgaatcg cggtccttac cagtccaagc ggagcggtta tccgcgatat catcacgacg
     1441 gtgaaaagaa gatatccaat tgtccaattg gttttgttcc caacagttgt acaaggaaac
     1501 caagcagcgg acaatgttgt caggaatatc cagagagtag aagagatggg caatttcgat
     1561 acaatgatca tcggtcgtgg gggtggttcg attgaggatt tatggccgtt taatgaagag
     1621 cgggttgctc gggcgattta tcaagctaat acgccggtga tttcatctgt aggacatgaa
     1681 acagatacca cgatcgctga tttagtagca gatgttcggg ctgccactcc aacagcagct
     1741 gcagaattag ctgttcctgt tttgtcagaa gagttgatgc gcattcaaga acggcaagct
     1801 agattgcagc aggcgtttgt acgccagatc cagcgcaaac aagaacggtt cgagcgtgct
     1861 cgacgttcct atgtatttcg gcagcctgaa agattgtatg aaggtcagac catcaaatta
     1921 gaccagttga aacagcgatt atatcaagct acacagcaaa tttatcatgc gaaagaaaaa
     1981 caaacgatcg cattaagcca ccaattggaa caagtggctc cgctttatcg agttaaagca
     2041 gctcgccaag aaactgctta tctagaaaaa cgcttagaag agaaaatcca acaatatatg
     2101 aaacaacagc agcagcgttt tgctcaagct gttcaatctc ttgatttgtt gagtccattg
     2161 aaaatcatgg gaagaggcta cagctatacg acgttaaatg atcaagtagt aaaaagtgtc
     2221 accgagattc atcaagggga tttgctgacg atccattatc aagatggaat cgttaaagga
     2281 caagtacaag agatagacaa aggagaataa aaatatggaa aatccaactt ttgaagagtc
     2341 actgcaagaa ttagaaaaaa ttgtcatgca gttagaacaa ggagacgttc cattagaatc
     2401 agctctagat tcgtttaaac gaggaatgga attaagcaaa cattgccaag atacattagc
     2461 taaagcagaa gagaccttga cgaaaatgat gacagaatca aatgaagaag ttgattttga
     2521 cggaaacgag gaaagctgag atgagattaa ctgacttttc agctattcat ctacctgctg
     2581 tagaaaaaga aatcttgtct tttttagatg agcatacaac ggatagagga ctctatgatg
     2641 cgatgagtta ttcagtgaaa gcaggcggga aaagaatacg tcccttatta cttttaacgg
     2701 ccgttgcttc ctttgatgag cctattgatg ttcccgtcta tcaagtagca gctgctttag
     2761 aaatggtgca tacgtattcc ttgatccacg atgatttgcc tgcgatggac aatgatgatc
     2821 ttcgtagagg aaaacctacc aatcataaag tattcggcga agcattagct atattggccg
     2881 gagatggttt gcttacagga gcattccagc taatcagtat ggcacactta ggtaattctc
     2941 ctaagcttct gctgcttcag caattagctg tttgtgcggg atcacaaggt atggttgctg
     3001 ggcaagctgc agatatcgag ggcgaatcga aaaagctttc tttagaagaa ttagcattta
     3061 ttcatgaaag aaagacaggg catttgattc gttatgcctt gctggctggt ggcatattag
     3121 cgaagcaacc tgaagaaatc ttattgctac ttcaacgatt agccgaacat ttaggtttag
     3181 cttttcagat aagagatgat ctactcgatg tgattggtac aacgaaaaca ttagggaaaa
     3241 cagctggtaa agatgaacgt atggaaaaaa atacttaccc gcgtttatta ggcctagaaa
     3301 agacacgtga ggcgttggag attgagctgt tatccgccaa caagatcata gacaaattag
     3361 aagaggacgt tttttctttt gatggcgggc tgatgcgcca gatgatcaag caatttgatg
     3421 tggagaaatg agggaaagaa tggaaaaaga acgcgtagat gtattagcgg tcaaacaagg
     3481 attatttgaa acaagagaac aagccaaacg ttcagtgatg gctggtttga tttacaacga
     3541 aaaaaacgag cggtttgata aaccgggaga aaaaattcca gtgagtagtg aattaagagt
     3601 caaaggtaaa aaacttcctt atgtttctcg agggggatta aaattagaaa aagcactcaa
     3661 agcatttgat ctatctgttg aaggaaaaac attgttagat attggtgcat ccactggcgg
     3721 atttactgat gcagctttgc aaaatggcgc aaaaatgagt tatgctttag acgttggcta
     3781 caatcagcta gcttggaaaa taagacaaga tcctagagtc gtcgtaatgg aaagagtgaa
     3841 ttttcgttat gcaaaacccg aagactttac cttaggaatt ccagaagttg ctgttatcga
     3901 tgtttctttt atttcactaa aactgatgct gcctccatta catgctattt taaaagaaga
     3961 gggagaagtg attgctttaa tcaaacccca attcgaagca ggcagagaag ctgtagggaa
     4021 aaacggaatt gttcgtgacc ctcagaccca tcaaaaagtt ttagaagaca ttttgtcatt
     4081 tgcggcacaa ggtggatatg atgtgctgga attaagttat tctccgatca cggggggaga
     4141 aggaaatatc gagttcttgg ctcacttgag gaaagtaccc gaaagcggaa caatcaattc
     4201 agctattaat atggcagaag tggtttcaaa tgcacacgaa cagtttgacc ataaataaat
     4261 tgtaactgtg aaaatcaagt gagaagtatg cggattcttc tttattatac caaggaaata
     4321 agctatcatt gattaagaag aatatgctaa ggagcgaaga cgtaatgaga aaaaaagatc
     4381 ggcatcgttt gattactcga ttgttgagtg agcaagatat ccgaaagcag gaagaatttg
     4441 ttgaaatcct gaaaagcaaa ggaatatctg ttactcaagc tacgatttcc agagatataa
     4501 aggaactgaa actgatcaag gtaccggctt cagatggcgg ctaccgttat agcataccag
     4561 ctgaaacaaa cgaagatgta aatactaaat tggaaaaact attaaaagac gctttcgtgg
     4621 cagttgacca aatggaaaaa tttgttattt tgaaaacatt gccgggaaat gcttcggcag
     4681 cagcaaattt gattgataaa cgttacaaga aagaattatt ttcgattatc aatgatgacg
     4741 ataatgtttt gatgattaca cgaactgaag accaggcagt tttattgaag aaagacttcc
     4801 tgcattacct ttaagttgag gagcgaatgt gatgctacaa gaaatcagta ttactaactt
     4861 tgcgattatt ccggaattgc ggctttcttt tcacgaagga atgaccgcgc tgaccggaga
     4921 gactggtgcg ggtaaatcca ttattatcga tgctttaggt ctgttagcag gaggaagagg
     4981 ttcaagcgat tatatacgtc aaggagcaga aaaatgtgtg cttgaagggt tgtttgaact
     5041 accaaaacaa gaaggattct ctgaactgat ggttgaatta gggatcgaaa cagatgagga
     5101 caatctgatt gttcgacggg atatgtcctt aactggaaaa aatgtttgtc gagtgaatgg
     5161 tcatattatc acattagcta atctgagaag aatcggcagt tatttagtag atatccaagg
     5221 acaaaacgaa catcaggaac tgctccagcc tgaatcacat cttgctttat tggatcgttt
     5281 tggagatgta gctttccaac agaagaaaaa gagttatcag caagaatata tctcttatcg
     5341 tgagctggaa aaaagggtac gaaaagtgca gcaaaatgaa aaatcctatg ttcagagaat
     5401 agatatgctc catttccagc aagaagaaat agctgctgct gatttgcaag tcggagaaga
     5461 agagaaatta aaagaagaaa gagaaaaact tagcaattat caaaaaattg ctgatggatt
     5521 agctgcaggt tacggagcac tgacgaataa tgaacaaaac agtgtagatg gtgttggctt
     5581 ggccgtttca gaactgcaag ggatcgccca tctagatgtg gagtatgaag caatttatga
     5641 aaatatccaa agtgcttact atttgctgca agatgcaatc ggcgatatga gtcgtcagat
     5701 tgatttattg gagttagatg agagtcgttt agaagaagtt acgcaacgtt tagaattaat
     5761 ccgacagttg aaacgtaaat acggcgaatc cattgagtca atattggctt actatgacga
     5821 aatcaccgaa gagctagctt cttctgattt ttcagaaggt caattggaca aaatgaaaga
     5881 ggaactagag caaaaagagc ttctacttca gcaacaagcc gcagatcttc atgaagcgcg
     5941 taaaaaaatc gcaaaagaat tggaaaaatc cattctgcat gaattgaaaa gtctgtatat
     6001 ggaaaacact gagtttgagg tacgtttctt aaaagaagaa aatagacaat tgaatcatga
     6061 tggatttgac caaatcgaat tttacattac gaccaatcca ggagagccac tgaagccttt
     6121 ggtgaaagtc gcttctggtg gcgaactgtc tcggatgttg cttgctttga aaactatttt
     6181 ttcttctgag caaggtgtga caagtattat ttttgatgaa gtggatacag gtgtgagcgg
     6241 aagagttgca caggccatcg cagataaaat ttctcaaata tcgaaatact ctcaagtttt
     6301 gtgcatcact catctgcctc aagttgctgc agtcgccgac tatcaatatt atattgtgaa
     6361 agcagtgatt gatggacgta cgcaaacttc tgtttcagag ttgaaaacaa aagaaagaga
     6421 agaagaaatt gcacgtatgc tggctggaag tgagattaca aaactcacac tcgaacatgc
     6481 aaaagaacta ttgaaactcg caaaaaggac ctgagccacc tcgcagtggt tcaggtcctt
     6541 ttcagcagct gatagctata ttttattttt acatgacact cattacagca gtcaatacaa
     6601 cagatccaat cgttaaaatc agcattgtcc aaatgacaaa ttttgttact ttagcaaaag
     6661 tacttgtttg ttttttttca cgcattgttt tcaacctcgt cattaattag ttgattcaag
     6721 tgtacaatct tttttccatt tccacaagag ggctttttta taaaaacttc ccttttttta
     6781 aataataggt agttacgata caaataaata atgttaatag aactagccat ataaaggcat
     6841 catccctttc agcaataggt aacttaatgt tcattccata gatactgccg acgattgtcg
     6901 gtatagttaa aataatcgtt aaagaagtta aaattttcat aatattattc agattattcg
     6961 atacgattgc tgaaaaagtc tgattcattt gttctactag ttttaattga atgcgagaag
     7021 tagtcattgc ctgctctgtt tccaccaata catcacgtaa atgatttttc aaagcatgat
     7081 tttcttggaa aagtttattt tgcgctagtt ctttcaatgc atctaaatta gcagaagtcg
     7141 cttctctgaa ataaacgagg cttttttgga tatccatgat ttgataaagt tgtttgtttt
     7201 cagtggatac ttgaagtttg gcttccaaag tatcgcattg agattgaagc gtagctaaat
     7261 aactgttgta agagagtgtg acctgccaaa tgaattgaaa gaagatattg agattaggcg
     7321 aaatatcact ttttggaaag gtctggctaa agactttctt taaaaacagt ggttcatgat
     7381 tcgtaattgt gataagcttc tttttgggcg tcaaaataat agagagcggg tatgtatcca
     7441 cttgaaaata accgctagga cttgttttag caaaaggata ttgtaacaat aataacgctg
     7501 gctttgtcaa ctttgattga ttgaattctt ctgtacggga attttctgca tcgtccaata
     7561 tggaagtgat gtaatctgac ggaatttcaa attgatgtgt cagctgatga atctcttctt
     7621 gattaggacg ttctacacaa taccagatcg tttggtcagt ttcctttccg gtattacgca
     7681 gtttctcatt atctaatgta aaatatttga tcatgatttt cctcctgttg cctgtttctt
     7741 ttattataac ggatagggaa ttggaataca tggaatgtgt ttgtaagata tagaatataa
     7801 ctgcataaat ttaaataaaa tgaaacagtt gtgtaaagta tatacagtat acttgaagca
     7861 acgataaaaa aggagtttac gttcatgggc acattcacac cacttatctt gttttggtat
     7921 atttacccgg taatcgtttt gtttggttgc caatttcttg taaaattatt ttcgttaaat
     7981 cgtcgtttta aaataaaagc ccctgatttg gcgattcctt ttttgtgggg cggtatgcat
     8041 gcattgtcta gaaatacagg gacaatttct atcttaccgt ttttctttat ttctgttttg
     8101 ctaatgggaa tttgtattgc tgttttccaa gcatattatt atgaagaaat tatttatccg
     8161 cgttatttca agatgacttg gcgtttggtt tttctgttga caatcgtctt gtacgctgtc
     8221 ttgatcattg tgaacatact ttcttaccta tgaaaaaaat agaaaagctg taaaaagtct
     8281 tttaaaaggc tttttgcagc ttgattttta aaaaaatgtt tatgaataaa aaaagttgtt
     8341 cccggatttc ccagaaaagg tggtagaaag tgggggattg tggtagacta attttagtta
     8401 gtggaggaat ggggggcttt caatgttgat gggtgaattc caacataata ttgatgcaaa
     8461 aggacgttta atcgtgccct caaaacttcg tgaagaactt ggcgaaaagt ttgttctgac
     8521 acgcggattg gatggctgtc ttttcggcta tccgatgagc gaatgggaaa atctggaagc
     8581 taaactgaac gaaatgccgt tggctaaaaa agacgcacgt acttttgtgc gattcttcta
     8641 ttcggctgcc actgaatgcg aacttgataa acaaggacga atcaatatac ccagcacgtt
     8701 aagaaattat gcagctttga caaaaggttg cgtagtcatc ggtgtatcta accgaattga
     8761 aatttgggat gagacacgat ggcaagattt ttctgcagca gcagaagaga actttgacga
     8821 aattgctgaa agtatgattg atttcggact ataagaatag gaagaggctg actaatgact
     8881 gaaacatttc aacactatac agtcatgttg aaagaaacgg tagatggatt gaacatcaaa
     8941 ccggatggcg tttatgtaga ctgtacactc ggcggtgccg gacatagtga atatttatta
     9001 agccaactaa gttctaaagg gcatctgtat gcgtttgacc aagatcaaaa agcaatcgat
     9061 cacgccaagc tgcgtttagc ttcttatgtc gaacaaggta tggtcacatt tatcaaagcg
     9121 aacttcagag aattagaaga acgcttgagt gagcatgtag ataaggtaga cggcattttg
     9181 tatgatctag gggtttcctc tcctcagcta gatgaagcag aacgtggatt tagttatcat
     9241 caagatgcac cgttggatat gcgcatggat cagtcagctc ctttatccgc ttatcacgta
     9301 attaatgaat attcctacca tgaattagta aagattttct tccgctatgg cgaggagaaa
     9361 ttttccaaac aaattgctcg tgaaatcgaa cgagtacgaa aaacaaaacc aatccaaaca
     9421 actggtgagc ttgtcgaaat cattaaacaa gtcatcccag ctcccgcaag aagaaaaggc
     9481 gggcatcctg caaaaagaat ttttcaagct gtgcggattg cagtaaatga cgaactcggt
     9541 gccgaagagg cctctttaga acaggcaatc cggttattga aagtcaatgg aagaatcagc
     9601 gtaatcacat tccattcatt ggaagacaga attgtcaaaa gtatgtttaa agaatatagt
     9661 acggtacaag atctaccacc gggacttcct gtcatacctg acgaattcca accagaactg
     9721 aaagtcatta ctcgtaaacc aatccttcct tcagaggaag agctaacaga aaacaatcga
     9781 tcacgcagtg caaagttacg tattgcagaa aaaataagaa tgaaagagga gtagaatgat
     9841 ggcagaatta aagaaaatgc aagattacga atatgattta cctgaattgg tggatcagcc
     9901 ggaacaacaa atgcctgatt cttcttcgaa tcaagaaatc attcttccac aatcaccggc
     9961 aaaacgatta aagcagattt ctgctttgga aaaaattgct gtggcaagcg tggcgattgc
    10021 tattgttgct ttatgtgttt taacagttat gctgcgcaca aatatcagcg gagctgaaaa
    10081 agaaatcacg tcaatacaaa cagaaatcaa tgataaaaag caagaaaaaa cgagtttaga
    10141 acaagaaaaa aacgaacttt ctcgtactga acggattaaa caaatcgcag aagaaaaagg
    10201 attgtctatt aatgacgaca atttaaggaa agtgaagtaa atgagtctta aaaataaaat
    10261 tcggcggttt atggaaaaga aaaatctaaa tccgatgaac aatcgcaaaa aagtaggtat
    10321 cattttattt gctacgagta ttggattgtt ctttttattt gccgtccgtt tctcgtatat
    10381 cgttattgga ggtcatgttg ctggtacctc attagctgaa aaaacaaaac agttgtacca
    10441 aggaagtgaa gtagtaaaag caaaaagagg aacgatttat gatcgtaacg gtgtggcgct
    10501 cgcagaagat gcctcctctt attcaattaa agcgatactc tcaaaaacat atacctctgg
    10561 ggataaaaaa ctgtatgttg aagaaaagaa ttttgataaa atagcagaaa tcctgcataa
    10621 gaatctttct atagacaaaa aagatgcact gaatatttta gaggacggcg caaaaaaaga
    10681 gctgtatcaa gttgaatttg gtagctatgg gaaaaatatc tcccaagaaa ccaagcaaaa
    10741 catcgaagcg gacatgaaaa aagaaggtgt cgctggtctt tattttgtcg accatcaggc
    10801 tcggatgtat ccaaatggcg tcttttcttc tcattttatt ggatacgcag ttccagacaa
    10861 agatgagaat ggcttagttg gtaaactagg tttggagtct gcttacaatg atatcttgag
    10921 cggtaaagat ggtaagatta tttatcaaaa agataatttt caaaatcctc tgccaggtac
    10981 agtggcagaa gaagaaaaag ctgtcgacgg acaagatatc tacacgactt tagacagcag
    11041 actacaaagt tatttagaaa cactgatgga tcaagtaaat gaagaatatc agccagaaga
    11101 attgacagca gtcctgatga aagcaaaaac cggagaaatc ttggcaatgg gacaacgacc
    11161 tactttcaat cctgaaacaa tggaaggatt gactggaaaa gatgctatct ggcgtaattt
    11221 cttggtacaa gatagttatg aaccaggatc aaccatgaaa gttttcacta ctgcagctgc
    11281 tatcgaagaa ggagaattca acgagaatga aaccttccaa tctggaaaaa tccaagtaga
    11341 agatgctacg atcaatgacc atgactttgg tgaaaaaggt gtgctgacca tgcgtcaagc
    11401 tctttcatgg tcaagtaacg tcgggatggt catactggaa cagcgattag gcggacgatg
    11461 gtataactat ttacaaaaac tgggatttgg acaaagtact cattctggat tagatgatga
    11521 agtaaacggt gctttaccta cattaaatat tgtcgaccga gcaatgagtg cttatggaca
    11581 agcggtaggt gtgacgaatt tccaaatgat gaaagctttc acttctattg cgaataacgg
    11641 tacaatgatt caacctcgat atatcagcaa agtggtggat ccccaaactg gagaagagcg
    11701 aacaactcaa acagaagttc tagggcaacc tttttcaaaa gaaacaacag aaaaagtccg
    11761 tgaatatatg agagatgttg tcgaaagcga aaactacggt agtgcttatg gtgtatatag
    11821 tgtacctgga tacaatgtat cggcaaaaaa cggaacagcc caaatcgctt ctgatacagg
    11881 gggatatcaa acaggagata cagcctacct gtattcgatt gtggagatgg ttccttctga
    11941 agatccagac tatgtcttgt atctaacaat gaaacaccct aagacatatg atcgaatggc
    12001 attggctaaa atcgcaaatc cattgatgaa acgggcaatg gattttaaag aaactgaaga
    12061 agattcagat acagaaacaa aaacagaaaa agtatccgta gccgattatc gtaatcttga
    12121 agcggacgtt gcagcagctg atgctcaaaa aagtggactg caacctgtag tgatcggtaa
    12181 tggtaaaaaa gtacaaaaac aatctactgc caacggtgat cagttgatct ctggagaaaa
    12241 actgatccta tatactggtg gtgataagct gatgcctgac gtaacaggat ggtcaaaagc
    12301 agatatcatg aagctgggga aaatcctcgg tattgaagtt agttttgatg gagatggtta
    12361 ttgtgtcaaa caagaacttg ctccatatga aaaaatcact gaagacaaac ttagttttac
    12421 tttagaagaa taagaagaga aataggagag aaaagagcat ggaatggaca caagcattga
    12481 ttcccattgt cagcagttgt gcaatgacaa ttgccgcaat gccgctattt attggttatt
    12541 ttcaaatgaa aaagcaaggt caggcgattc gtgaggaggg accaaagtgg cacaatagca
    12601 aagccggaac tcctacaatg ggcggcttag tgtttctgat cggctctatt ttgacaggaa
    12661 tttgggttgg agcttggcaa aaacagctaa ctcctactct tttcatcttg ctttttgtat
    12721 tagcgttata cggagtaatc ggctttcttg atgattttat aaaaatattc aagaaaagaa
    12781 acatgggact gaactcgaag cagaaattga ttggacagat tctaggggga atcatctttt
    12841 atcttgttta ccgttctgaa ggttatccag gtgtattgaa tttcttcgga ctagatctac
    12901 ctttaggaat catttatggt attttcgctc tcttctggct tgttggattt tcaaatgcag
    12961 taaatctgac agatggcatc gatggattag ttgctggctt aggaagtatc tcttttgcag
    13021 cttacggtct aattgcttgg catcagcatc agtatgatgt attaatcatt tgcttaagtg
    13081 ttttaggagg tttactgggt ttcttcgtct acaacagaaa acctgccaag atttttatgg
    13141 gagatgtcgg ttcattagcg ttgggcggac ttttagcggc aatttctata atgctaaacc
    13201 aagaatggac attgcttttg attggtttga tctatgttat ggaaacagca agtgtgatgc
    13261 ttcaagttac ttcctttaag cttacaggaa aacgaatttt taaaatgtct ccaattcatc
    13321 atcactttga aatgtgcgga tggtcggagt ggaagattga tacagttttt tggttgatca
    13381 gcattgtgac ttcgttgatc acattatgga tcgtttggta ggagatgacg ggaaatgaaa
    13441 aaaatatcaa catatgaaaa taagaaagtg cttgtcttag gactcgcaaa aagcggagta
    13501 agtgcggcca aattattaca tgaattgggc gcacttgtca ccgttaatga tggaaaacct
    13561 tttgacgaaa atccggaagc acaagagctg ctttctttag ggataaaagt aataacaggt
    13621 agtcatccaa tcgaattgtt ggatgaagaa ttttcattga tggtcaaaaa tccagggatt
    13681 ccctacagtc atcctttagt agcaaaagcc caagaaatgg gtattccggt aattacggaa
    13741 gttgagctag cttacgaagt agcggaatgc ccaattattg ggattacagg aacgaacgga
    13801 aaaacaacga caacaacgat gacaggacta ctactgaatg caggagcaga tcaaggaatt
    13861 gcacgtttag cgggtaatat tgggtatcca gcaagtggtg tagcgcaaga agcaaaatca
    13921 gaagataaaa ttgtgatgga attgtctagc ttccaactca tgggaatcac agatttccgc
    13981 cctcatatag cggtgattac caatatctat gaagcacata ttgattatca tggtacaaga
    14041 aaagaatatg tcaaagctaa atggaacctt caaaaaaaca tgacagaaaa agattatctg
    14101 atccttaact ggaatcagtc agaactacaa gaattagcgc aaagaacaaa agcacgcgta
    14161 ctgcctttct cgacaaaaga agtgttggaa gacggtgtgt atgctgacga ttactcaata
    14221 tactataaaa aagaaaaaat aatggaaata tctgaacttg gcgtaccagg taagcataat
    14281 gtagaaaatg cactagctgc tatttctgta gcaaaattgt atggtatatc aaatgaagct
    14341 atacgagaaa cgttgcattt tttccatggc gtaccacatc gtacgcaata tgtaggagaa
    14401 atccaaggca gaaaatttta taatgattca aaagcaacca atattttagc gactaaaatg
    14461 gctctaagtg gatttgaaac gtctaaggtc gtgttattgg ctggcggatt agacagagga
    14521 aatacatttg atgaattaat tccttcatta aaaggaatca aagcaatggt cgtatttggc
    14581 caaacaaaag aaaagttgat ggatgctgga aaaaaagcag gcatcgagac aatagtaaca
    14641 gccgattctg tagaacaagc agttccttta gcattagaaa acagtacaga tggtgatgta
    14701 gttttattat caccagcgaa tgccagctgg gatcaatacc caaattttga aactagagga
    14761 aacagattca tggaagctgt gaaccgatta aaatagaaaa agtagaagcg agcgataaaa
    14821 aatgaaaatt ttggttacag gcggaggaac agggggacat atttatccag ctcttgcctt
    14881 tgtcaattat gtaaagactc aagaaccgaa tgctgaattc atgtatgtgg gagcaaaacg
    14941 aggtctagaa aacaaaatcg tccctgatac aggaatgcct ttccatacct tggagattca
    15001 aggatttaaa cgaaaaattt ccatgcataa catcaagacg gtccgattgt ttcttaagag
    15061 tatccgtcaa gcaaaaaaaa tcctcagtga ttttcaacca gatatcgtta tcggtactgg
    15121 aggatatgtt tccggagcgg tagtgtacgc tgcagccaaa atgggtattc caacagttat
    15181 ccatgaacaa aacagtgtac caggtgttac caataaattt ttaagccgat atgtggatcg
    15241 gatcgctctt tcatttaaag atgcggcttc cttttttcct gaaaacaaag cagttcttgt
    15301 aggtaatcct cgagcacaag aagtagcaga tactaaaaaa tcagatattt tgaaaacatt
    15361 tgatttggat ccagaaaaga aaacagtctt gatttttggc ggtagtcaag gagcacttaa
    15421 aatcaatcaa gcagtatcag tttttttaag tgaatatatt ccttcttctt accaagtttt
    15481 gtatgcttca ggagatagat attatgaaga gatcctaaat aaaataaatc ctgaaataca
    15541 aaatattagt gtccagcctt atatcaaaaa tatggcagag gtcatggctt ccagtgatct
    15601 gctggtgggg cgtgctggtg caacttcgat cgcagaattc acagctctgg ggttaccggc
    15661 agtgttggtt cccagcccat atgttacgaa tgatcatcag actaaaaatg caatgagtct
    15721 tgttcacgca ggagcagcaa aaatgattgc agataatgaa ctaacaggtg aatcactaag
    15781 tcagacagtc aacgaaatca tgggtgatga agaattgcaa aagcaaatgt gccgagcttc
    15841 taaagaacaa ggaatccctg atgcttcaaa acgtctttat gacttagtaa aacaaataat
    15901 caaaaaataa aggcggtggt cttagtgagt aagaaaaaag aaccaaaaaa attgtctcat
    15961 ccgtcttctg atgaaaacaa tttgactcct tggcaaaagg ctaaccgaaa atatctggca
    16021 gaacaagaga tgaaaaacca agagccaaaa aaagaagaca taacattaga tacacccctt
    16081 tcagattctg aagaagaaaa aacggttcaa gaggaacagc aagaagaaag ccagtccgag
    16141 aaaaaaatcg aattttttga agagatcaag caaaagaaaa acggtggacc atataacggt
    16201 tcttttttga accggctgcc caatttaaaa agtcaacggc acaaagtact atttcgtcgg
    16261 ttagctttga tcatcactgt attgggtata ccgctgattt ttttgatcta ttatgtttct
    16321 ccttatagca aattacaagc agttgcagta tcaggcaata agacagtcaa cagccaagaa
    16381 atcatctctg atacaaaatt gtcattagga gaaaatgttt ggggacagta ttttcatcga
    16441 tctacttata tcgatcgcct taaaaaagca cagccaagaa tcgaaacagc aaatatacat
    16501 ttcaaaggga tgaatgaatt cgatttggat gtaacagaat ataaagagat tgctttgatc
    16561 gcaaaaaaca atcaatacta cccagtgatt gaaaatggaa cagtattaga tgaaaaagta
    16621 gcaaatccta caaaaaatct ccctatcttg gaagagttca aagactctgc aaaaatcaaa
    16681 gaattaacaa agcaatacaa tcagttatca tcagaattac aaaaagcaat ttctgaaatc
    16741 aagtacacgc caagagcaag caataaaaat ctgatccaat tgaatatgaa tgacggcaat
    16801 caagttattg taaatatcaa taatcttgcg aaccaaatga aatattattc gcaagtagcc
    16861 aaagatatgg atgaaaaagg tgtgatcgac atggaagttg gtatcttctc gcatccttac
    16921 ggtagcactc aagaaacgga agaatcgggc caatcggaag aaacaactga aagtgaagaa
    16981 aatgcggatc aaacaaacga atcaagtgaa gaggcagaaa cgcaaaaagc acaaacacct
    17041 ggatcgtcta gcacaaacgg atcatctgat tcaaatgatt caagtgaaac aagtcaaata
    17101 gaagataatc caagtacaac tccgtcaagt ttaaatgaaa attgataaaa caactttaaa
    17161 aagtaagaat gtactttcta aaaaccagta atttatggta gaattggaag cagtgaatat
    17221 atctattaat aagtatattt taaaacggac gctagttgtt cgcttgtata tggaatagta
    17281 agtaggaggg aatcccattc atggcaaaaa caggaatgta tgtaggcctt gatattggaa
    17341 cgacatctgt taaagttgtc gtggctgaat atatcgacag ccaaatgaat attattggag
    17401 taggaaacgc aaaatcagaa gggattaacc gaggcattat cgttgatatc gataaaacgg
    17461 tccaagcgat acaacgagca gtacgacaag cagaagaaaa agcaggtatc cagatcaaag
    17521 gcgtaagtgt cggtttgcca gcaaacatgc ttgaagtaga aaattgccag ggaatgattg
    17581 cagttaacgg agattcaaag gaaatcaccg atgaagatgt cagaaatgtg gcttcagctg
    17641 cccttgttcg ttcaatacct cctgaacgtc aaatcgtgtc aatattgcca caagatttta
    17701 ctgtcgatgg gttcgaggga atcaaagatc ctcgtggaat gatcggcgta agattagaaa
    17761 tgtacggtct gttgtttaca ggacctaaaa cgatcatcca taacatacgt aaatgcgtgg
    17821 aaaatgccgg attaatcgtc aacgaaatgg tgattactcc tttagcgctg actgaatcaa
    17881 ttctttctga cggagaaaaa gattttggta cgatcgtcat tgatatgggt ggcggccaaa
    17941 caacaacggc tgttatgcat gataagcaat tgaagtttac caatcttgac caagaaggtg
    18001 gcgaatttgt cactaaagat atctctattg tattaaatac gtcatttaac aatgcagaag
    18061 cgttgaaaat aaactacggg gatgcatacc cagaacggac atcaccagat gaagaatttc
    18121 cagtagatgt gattggtcag tcagaaccag tgaaagtcga tgaacgctac ttgtcagaaa
    18181 ttatttcagc tcgaatggaa cagattttca acaaagcaaa agatgcattg gaccaaatcg
    18241 aagcattaga gttgccaggt ggcgtcgtat tgacaggcgg agctgctagt cttcctggtg
    18301 ttgtggactt ggcacaagaa atcttcggcg taaatgtaaa attgtatgtt ccaaatcaca
    18361 tggggctgag aaacccggta tttacaaatg taataagtat cgtggattat tcagcaaact
    18421 tgagtgaagt gtatcaattg gcaaaaattg ctgttacggg tgaaacctca gcagctcgtc
    18481 aaatggtagt tgaacaagaa acaacaaaca catatgagag ctatgaagca cctgaagaaa
    18541 caatctacga tgagccggaa ccaaaagagt ccggagaaaa tgtaaaaaat aaaatcaagg
    18601 gtttctttac aaatattttt gattaattca tcctgggagg aataagatat ggaattctca
    18661 attgataata atatcaatga cggcgcagta atcaaagtca tcggagtcgg tggtggcggt
    18721 ggaaatgctg taaaccgaat gattgaagaa aacgttaaag gtgttgaatt cattacagcc
    18781 aacacagacg tgcaagcatt aaaaaattca aaagctgaaa cagttatcca attaggcccc
    18841 aaatatactc gtggtttagg tgctggttct cagccagaag ttggacaaaa agcggcagaa
    18901 gaaagtgaac aatccttacg cgaagcttta gacggtgcag atatgatttt catcactgca
    18961 ggaatgggtg gaggaaccgg tacaggtgct gcaccaatcg ttgcaggtat cgcgaaagaa
    19021 ttgggcgcat tgactgttgg tgtcgtgaca cgtccattca cttttgaagg accgaaacgt
    19081 ggccgttttg ctgcagaagg tatcgctcgt ttgaaagaaa atgtggatac attattaatt
    19141 atctcaaata accgtttatt agaagttgta gataaaaaga caccgatgct tgaagctttc
    19201 cgtgaagcag ataatgtttt acgacaaggt gtacaaggta tctctgattt gatcacagcg
    19261 ccaggttacg ttaaccttga ctttgctgac gtgaaaacag tgatggaaaa ccaaggtaca
    19321 gcattgatgg ggatcggtgt ggctagtgga gaagatcgcg tagtagaagc aacgaaaaaa
    19381 gcaatctcct ctccattgct tgaaacatct attgatggag ctgaacaagt acttcttaac
    19441 atcacaggcg gtttggatat gacattgttc gaagcacaag atgcttcaga tatcgtagca
    19501 aatgcagcaa ctggcgatgt aaatatcatt ctaggtacat ctatcaatga agaaatgggc
    19561 gatgaaatcc gtgtgacggt catcgcaaca ggtatcgatg aatcaaaaaa ggaacgcaag
    19621 tctagccgac cagctagaca agcacaaatg caatcacctg ctcaaaagac tgtattagat
    19681 atggatcaag ctaaaccaac ttcttcagaa gaagaaaaca gttttggcga ttgggatatc
    19741 agacgtgaac aaaatgttcg tcctagagtt gatgattcca attttgacaa tatcgaaaaa
    19801 aaagaatttg atacattcaa ccgcgaagaa acaaaatctc atggcgatga tgaattaaat
    19861 acgccaccgt ttttccgtcg taaaaaataa aggagaggaa gagcacatga ttgctgacaa
    19921 cttgcagaaa atcaagcaag aaattcagga ttcgtgtgct cttgttcatc gtgatccgaa
    19981 agaagttaca ttagtagctg taacaaaatc tgttgaatcc gctcaagctg aagaattggt
    20041 tagagctggc gttatgaatt gtgcagaaaa ccgagtagat aaattattag aaaagaaaca
    20101 agatttattt gattttcctg aaataaagtg gcatttaatt ggtaatttac agcgcagaaa
    20161 ggtaaaattg attgtaaatg aaattgatta ctttcatgcg ttggacagtt taagattagc
    20221 agaagaaatc cagaagcggt tagagaaaga attatcctgt tttattgaag taaatgtttc
    20281 tggtgaggaa tcaaaacatg gaatttcacc agatgagctt ttaccgtttg tggaatcttt
    20341 agctgcctgc acaaaaatca aggttgttgg gttgatgact atggcgccta aagaagcttc
    20401 tgatgagcaa atccataaag tgtttggtac gctgaaagaa ttgcgtgatc ttgttcaagc
    20461 aaaaggttat gcccatgcac catgtagtga attaagtatg gggatgagtc aagactttca
    20521 aattgcgatt gaagaaggtg caacatttat ccgtgtagga acagcgctat tcaaagacga
    20581 ataaaggagg aacaaaaatg ccgcttttaa acagaggagc aattgctaat tttttcggat
    20641 tagctgatga ggaagagtac gaataccaag attatcctga aacaacaaaa aaggctgtac
    20701 agccaactaa aaaaaataca acacagaatc aatcaactca ttctgtatct gctgcgaata
    20761 ctcatgaaaa acaaaaaacg gcgacttcac gtcctgtaac gcatgaaagt attcagccgg
    20821 ctgaaaagaa aaaagtaatt gaactacatc aatcacctac aaaaactgta gcgaaagaaa
    20881 gaacagcttc aagaacctct acacaaccta aagcaaatct tgctggaaaa atcactgtaa
    20941 tggaaccaag agcttattct gaagctatgg cgattgctaa acgaatcatt gctggagaag
    21001 caaccttagt gaattttcat ttagtagaag aaaaacaagc gagaagaatc gttgattttc
    21061 taacaggaac agtttatgca ctagatggag atatcaaacg tgttggagat gagatttttc
    21121 tttgtacacc gactgggatg gagattgaca gttcaaccgc tcaatctttt gcagatgcga
    21181 atttgtttga tctgtaagag gaggaactac gatttatact attgcactat tacttcattt
    21241 acttcaacgg gcagcctatt tctatagcat tatcttgatg gtatatgcat tgctctcttg
    21301 gtttccaggc gggtatcaat cagggctagg acgattatta gctaagatct gcgaaccgta
    21361 tatcagtttg ttcgatcgtc tgcctttgag gattggaccc ttggatttca caattttagg
    21421 ggcaatcctg atcttgaact tagccgtaca aggtttagca cagatcgtta tatggataac
    21481 tgtccattta gtttattgat tgaagcaaca aaatctttat tctcagaata gataggaggg
    21541 gaaaattttg gatgcaaatg tataccagca ctttcgggca gatgaacgta catttatcga
    21601 ttcggtaggc gattggattg aacaggttca aggacaatat gcaccctatc tgaccgaatt
    21661 tcttgaccca agacaatcct atattttaga aacactgatt cgacaaagca gtgatttgcg
    21721 tttcatgttt tatggcggat atgaacaggc agaaagaaaa agatgtttga ttttccccga
    21781 ttattacgaa cctgtagagg aagattttga aacagcattg tatgaaatcc attatcctag
    21841 caaatttgcc agcctaagtc atgggaaaat ccttggaact ttagtgggaa cggggatcaa
    21901 acgctcttac tttggcgata tcatctcgga cggtgatcgc tggcaagtat ttattgcaaa
    21961 tgaggtggat cattttgttg ctgcacaagt aacaaagatt ggaaaggttg ctgtacgctt
    22021 ggaaccaatc aaatacacag aaatgattat gccgaaagat agctggacac aagaaagagg
    22081 aacagttagt tccatgcgac tagacagtgt gatctctgag atttataata tttccagaca
    22141 gcggtccaag cagctgatcg aagctggtaa agtaaaagtg aactggacag aaaatacaag
    22201 acctgatttt gtacttgaac tattagatat tatttcagtc agaggttatg gcagagtcca
    22261 gatccaagaa cttgagggga agacgaaaaa agaaaagtac agggtactgt tcggtgtatt
    22321 acgtaaataa aataaaaagg tggtttactc atggctttaa ctccattaga tatccaaaat
    22381 aagacattcc cgacaaaaat gcggggatat aatcaagacg aagtagatga tttcttagat
    22441 ttagttgttc gtgactatga ggaattgact caaagaaatc gagaattaga aaaagcagtc
    22501 aaacattctg aagaaaaact tgagtacttc aatgaattaa aagatgcttt gaaccaatca
    22561 atcatcgtag ctcaagatac ggctgataaa gtaaaaacaa gtgcaagcaa ggaatccgaa
    22621 gtgatcgtta cttctgcaca aaacaaagca gatgagttag ttgcgaacgc tgaaaaacgt
    22681 gcacatcaat taacaactga tgcagaagaa aaagcaagaa aaatcttgac agatgcaaca
    22741 gaaaaagcaa gacagcttgc gacagaaact gaagatctta agaaaaaaac acgagtattc
    22801 catcaacgta tcagcttgat gcttgagtct caattagaac aagtgaaaag tccggaatgg
    22861 gatgaaattt tacaaccatt ctccagctac gtgactgaca gccatgaagt gatcaaagaa
    22921 gttttagcaa aacagcttga caatgaaaat gatactgatg taaactcaga cacaggagtc
    22981 atagaagcac ctatgacggc atttgataca caggctattg atatctcgat cgtgcctggt
    23041 gaaaatgaaa acgagtagaa cagaaaagca ctttgttatt cttaaagcga gccgatgaga
    23101 gtggaagttc ggtaagacac tgcatggtga aagatcctct acttgagacc tgcttttccg
    23161 aaattagagt aagaagcagc ggctgacctc gttattggtc acagagagtt tgcactagat
    23221 agatgcaact aaatttgggt ggtaccacgg catgttcgtc ccttgcggct gaacatgctt
    23281 ttttgtgtcc ggaaaacaaa taaaggagtg tatggttgaa tgaaaatgaa agaaaccttg
    23341 caattaggaa aaacaaaatt ccctatgcgt ggtaatttgc caaaccgaga aggcgagtgg
    23401 caaaaagaat gggaagaaaa tcgaatctac caaaaaagac aagagttgaa cgaaggaaaa
    23461 cctagctttg ttcttcatga tggacctcca tatgccaatg gtaatatcca tttaggacat
    23521 tcattgaata aaatcagtaa agacattatc attcgttcaa aatcaatgtc tggctatcgt
    23581 gcaccatatg ttccaggatg ggatacacat ggattgccga ttgaacaagt attggctaat
    23641 aaaggcgtga aacgtaaaga aatgtccatg gcagaatact tggaaaaatg tcgtgaatat
    23701 gcactttccc aagtagataa gcaacgagca gatttcaaac gcctaggagt tgcaggagat
    23761 tgggaacatc cttatgttac attagatcct tcttatgaag ctgcacaagt acgcgtattt
    23821 ggtaaaatgg ctgaaaaagg ctatatttac aaaggattga aaccaattta ctggtctccg
    23881 tcaagtgagt cttctttagc ggaagctgaa attgaatata aagatgtaaa atctccatct
    23941 atttttgtag ctttcaatgt caaagacggc aaagatattc ttgatgaaga tacagctttt
    24001 gtcatttgga cgacaacacc atggacattg ccagcaaacc aaggtattgc tgtaaatcca
    24061 aaatttacgt atgtattggt ggaagccgat ggccgtaagt ttgtcgtagc gaaagatttg
    24121 ctagaaacag tccaatcaga aatcggttgg gaaaatgtaa cagttctaaa agaatttgct
    24181 ggacaagaaa tggaatatat gactgcaagt catccattct atgaacgtga atcacttgtg
    24241 attttaggcg atcatgttac tttagacgct ggtactggat tagtccatac tgctccagga
    24301 catggggaag atgactatat cgctggtagc aaatataaac tatctgtagt gtcacctgta
    24361 gactcaaaag gattgtttac agaagaagca cctggatttg aaggtatctt ttatgacaaa
    24421 gcaaatccta tgatcactga tctattaaaa gaaaaaggag ctttgctgaa gctagatttc
    24481 tttactcata gttatcctca cgattggcgg acgaaaaagc ctgtgatcta tcgggcaact
    24541 ccacaatggt ttgcatctat cgataaattc cgtcagaata ttttggatga agtagaaaaa
    24601 gtagagtggg ttatcccatg gggtaaaaca cgtttgtata atatgatccg tgaccgcggc
    24661 gattgggtaa tttcccgtca gcgtgcatgg ggagtaccac ttccgatttt ctatgctgaa
    24721 aatggagaag caatcatcac accagaaaca atcgaacatg tggcaaaatt atttgctgaa
    24781 catggatcaa ttatctggtt tgaaaaagaa gcgaaagaac ttcttcctga aggatttacc
    24841 cacccaggtt ctcctaatgg cgaatttaca aaagaaaaag atatcatgga tgtctggttt
    24901 gattctggtt cttctcatga agctgtttta cgtcagcgac cagaattatc attcccagca
    24961 gatatgtatt tagaaggatc agaccaatat cgtggctggt tcaactcaag tatcacgact
    25021 agtgtggcta tcaatggtgt agcaccatac aaatcagtta tttctcaagg atttgcttta
    25081 gatggtgaag gccgtaagat gagtaaatct ttaggaaaca tcattgcacc agaaaaagtg
    25141 atcaaacaat ttggtgcaga tatccttcgc ttatgggtat ctagtgtaga ttatgaagca
    25201 gatgttcgtg tttctatgga tatcttaagt caagtggcag aagtatatcg taaaatcaga
    25261 aatacgatgc gtttcttatt agcgaataca gaagatttcg atcctaaaaa agatactgtt
    25321 tcgtacaatg atttacgttc tgtagacaaa tatatgaccg ttcgattgaa ccaagtaatc
    25381 aaagaaatcc gagaagaagg gtacgacaaa tataacttca tgcatattta ccgtacagtt
    25441 atgaacttct tgacggttga tctatcgtca ttctacctgg attttgctaa agatgtagtt
    25501 tatattgaag cagaagatga ttatcaacgt cgttgcatgc aaactgtttt ctatcaaaca
    25561 gctgtagctt tgacaaaatt attgactcct atcattccgc atacagcaga agaaatctgg
    25621 tcattcctga aagaagaaga ggagtacgtt cagttaagcg agtttcctgg ttatgaagag
    25681 tttgcaaacc aagaagaatt gatggatacc tggactgctt tcatggactt ccgtgataat
    25741 gtcttaaaag cattggaaga agcaagaaac agcaaattga ttggtaaatc tttggaagca
    25801 aaactaactg tttatccaaa acaacaagtt cgtgaaatgt taaaagcttt ggatgcagat
    25861 attgcacaat tattgatcgt ttcaccagac tactttgaag ttatggcagc agatgaacaa
    25921 gtgccagata acgctatggt atttgatgat gttgctatta cagtggaaaa agcagacggg
    25981 gaaacttgcg atcgctgtcg ccaagttcgt aaagatgtag gagtagatga aaaacttcct
    26041 catctatgcg gtcgttgtgc aaaaatcgtt gaagacttct atcctgaagc tgtagctgaa
    26101 ggattcgaag aaaagtaaaa gggaaaaggg ggtgtgacat gttctttgtc acaccccctt
    26161 ttgttatcaa tcgtttaatt ttccacgttc acgataccag ttatctggtt gccagatcca
    26221 ttcgaatccg tctttttcaa gtagttcgaa agctgcttta ggtcccattg atccagcttt
    26281 gtagttggga aaatccggtt gtgtttggtc ccaagctttt cgaatgacat ccacgatatg
    26341 ccaagattga gcgacttctt cccaatgaga gaagttcgtt ccatcaccat tcaacgcatc
    26401 taatagtaat ttttcgtaag cttctgggct attttcaacc atttctgcac tatttctaaa
    26461 ttccaatttg attggttccg tttcaaatcc ttgccctact tctttcccat tcaaggataa
    26521 agaaaatcct tcagttggct gaatatagat cgttagaaca ttaggtggaa gtgtcgtatc
    26581 gtcacatggt tcatcgacag atgttttgaa tacattaaca ggaacttgtt tgaatacgat
    26641 gttgatccga gtcccttttt cagtcaaacg tttgcctgta cgtacataga aaggaacacc
    26701 tgaccaacgg aagttatcga tagtgaattt gcctgcgacg aatgtttcag tctctgattg
    26761 agcatccact ttgtcttctt cgcgatagcc cttgaattgt acaccatcca attcaccttg
    26821 agcatattgc ccgcgaacga agttttctaa cacttcttct tcgttataaa tgcggatagc
    26881 tttcaatgct ttgatctttt ctgtacggat ttctttttcg gaaaatgcta ctggcggttc
    26941 cattgcaagt aatgctacaa cctgtaaaat atgattttgc accatgtctt taagtgcgcc
    27001 actatgatcg tagtagcccc cgcgatcttc tacaccgagg ttttcgccaa aagtaatttg
    27061 gatattgtcg atatagcgat tgttccattg agcctcaaaa atattattgg caaaacggat
    27121 agctgagatg ttttggatca tttcttttcc taaataatga tcaatcctga aaatatcttg
    27181 ttctgggaat acttctctga tttgctggtt caattcaaaa gcagactggt aatcagaacc
    27241 gaacggtttt tctatgatca aacgattgaa gccatttttt gtcataatat tttgtgattt
    27301 caaatgagaa acgatcgttc caaaaaattg aggtgccata gccaaataat agacacggtt
    27361 accgtttaat tgatagtgtc catctaatcg gtcagacaat tcttttaatg tctgataatg
    27421 cgttgaatca tttacatcat gggattgata gtaaaaatgg cttgcaaagg tagttgcttc
    27481 ttcatctgaa ggattcaatg cacggattgt ttctttgaca acttcacggt agtgctcatc
    27541 gctccaggga cggcgagcgg ttccgattac agcaaaatgc tcgcctaaat ttccttttct
    27601 gtaaaggcgg aacaaagacg ggtacaattt acgctgggct aggtcacctg taccgccgaa
    27661 tatcgtaaac aatacatttt tttcattaga catgtggata cactccttta tattttatcg
    27721 acaaaaatcg tgctggccgc cttaaagctg acggggattt tctttctatc attttggata
    27781 gtgatcggtc cttcataagc tgcgatgtca acgatctcat attcttcgtt gatattcagg
    27841 tcaatagtga ctaagtaatc caacaattct ttttcatcaa gcactcgtgc gatacgcact
    27901 tttgtaccga caggatagga ctccaatgtt tgtcggcgtt cttcatggac aacttcctct
    27961 tttttaggaa tcgatcctcc atgaggacaa tgttcaggat gattcaaata gttttctaaa
    28021 tgatctgcaa gatgttccga agtcacatgt tccaatactt cagcatcatc atggacttca
    28081 ttccagctat agtctaaatg ttcaactaaa aaaacttccc ataagcgatg tttgcgaatc
    28141 agactgctag ctttttgcag tccgcttgca gtcaattgta caccttgata aggggaatgt
    28201 tctacaagct cttctttcac aagtttagaa atcatttcac tgacagatgc agctgagaca
    28261 tctagactag atacgatttg tttattgttg actttcgttg tatctccacc tagttctaaa
    28321 atgattttca aataatcttc tcgatttggt gtcatattcg atcatccttt catggtaaaa
    28381 aaccatcatt agtaagtgta acaaaaaaaa cgttaattga ctatttttct aaataaaaaa
    28441 caaaggattt ttagacgttt ttacaacaaa aaacgaaaga tgatagagaa gactcattct
    28501 ttcgtttata aaggatatat atatacattg ggagggatct atttttcaga taacaactta
    28561 tatgaagtgt ctgcagattc gtcataaaga gtttgttcgg gataagaaaa gataagataa
    28621 aaaccatact gatcacgtac gactaaacga tattcatcta tttctaccac agatgtgcca
    28681 agagactgac cgttgatcaa aaacgtgtaa tcagatgtaa tctgtaattg ttgtttttgc
    28741 tggctatcac taaagaacca tgtaccttga tactttgaga gtccttctga cttttccgct
    28801 tgttttttct ttttcaagta aataaaactt gagagaacgg taatgagtga accaagaaaa
    28861 tagatcattt tttttatcac caaatcactc ctttatgaga aaataatcgc atagacatat
    28921 tctttcttct ttatttcatt gtaccgaata aaagctaaaa tttctctaag actttcgact
    28981 ggttttttta ctgtttttct acaaattatg ataggataca tagtaggaag aaaggtgaca
    29041 taatttgaag tatcagcaag tggaaatagc tttttttatt gaccgcccga atcggtttat
    29101 agcacattgt atgaaaaaag acggagaaat aattattgcg catgtcaaaa atacaggtag
    29161 aggaaaagag gtcctgatac caggcgctga agtggctatt gtatttgctc ctggacctaa
    29221 acggaaaacc gcctatgatc tgattgctgt aaaaaagaag ggtgactggt ttaatatcga
    29281 tagtcaattg cctaatcggc tggcaattga tggtatttta gacggcacga tccttttacc
    29341 aggattaact ggtccattta cggtatacaa gcgtgaagta acttatcttc actccaaatt
    29401 tgatatttat ttagagacgg tggtcggaca aaaagcattt gttgaagtca aaggtatgac
    29461 acttgaaaat aaagcgattg gtgcttttcc tgatgcgccc acactcagag gattgaaaca
    29521 tataggagaa ttaacgtatg ccgcacaaga tggttatgcc gcttatgtct tgtttattgc
    29581 tcagttcgaa catcttcatc tggcaacgat ccatgaagaa atgcaaccag cactagctga
    29641 tatggttcga catgcgcagc aaagcggagt tcaaatcctc gcctataatt gtcaagtatc
    29701 ggcagatgag gtaactgtca agcaggccat tccatttgat gtagatatcc cttttgaaga
    29761 tcctatacaa tagaaagaag gtaaaataat gatcaactta ggtattatcg gaacaaattg
    29821 gattactcat caatttgtcc aagcagcttt aagcacaaaa aaatatgaat tgacagcggt
    29881 atattctaga catttggaaa aagcacagga atttggttct cattatgaag gggatatcga
    29941 attcgcaaca gatttgaaga cttttttcaa tattactcat atggacacca tttatattgc
    30001 ttctccgaat agtcttcact ttagtcaagc taagcaagca attttggcag gaaaaaatgt
    30061 gatagttgaa aagccagctt tttcgacgcc aaaagaaatg gatgaaatca tccatttggc
    30121 aaatgaacac aatgtgtatt tttttgaagc agcaagaaat atccatgaat caggttttaa
    30181 aaagattgcc gattacttac cattaaaaga cgagataatc ggtgcaaatt tcagctatat
    30241 gaaatattct tcgcgctatg accaagtatt agatggagaa gaaccgaata tcttctcgac
    30301 tcatttctca ggtggtgcat tgatggattt aggtgtttac cttgtatatg cagctgttgc
    30361 ttggtttgga atgccaaaag aagtccatta tttcccaaga aagatttcta ctggtgtaga
    30421 tggtcttgga tgggggatct tacgttatga gaattttgat gtatcgatcc agccaggtaa
    30481 aatcggtgat tcttatttac cgtcagagat ttattttgac tcaggaacgc tagtgatgaa
    30541 tggagtcaat gcaattgaac aagcacaatt tcatgatcgc gcgcataaag aaatcattga
    30601 tttggcggta acagctgcag aaaatccaat ggcagatgaa gcagaggatt ttgccaatgt
    30661 catggctcat ccgaaagatc cagcttgggg attgttgtat gaagagtgga tcgagctagc
    30721 tagaaatgtg aaccaagtga tttatgattt aagaaaaaat ggcggcatca aattcgatgc
    30781 agataatgag gaagattttt aatgaacgaa tttgtacaag tatttcccga aaattggcag
    30841 aaaaaatatg aggataaagg gtttatctca ccttctctta ttcaacgagc agtttttcag
    30901 ccactaagta ataaacaaag tatagtagcg gtttctccta caggttcagg taaaacactg
    30961 gcttatcttt ggcctctact actaaacgtg gaaccaggtg aagcaagtgc gttggtcatt
    31021 tttgcttctt ctcaagaact agctattcaa gtagcagatg ttgcacgtga atggggaaaa
    31081 gacaaagagt tgaaagtcca atctttggtg ggcggagcca acgtcaaacg tcagattgag
    31141 ggtttgaagg aaaaaccaga aatcttgatt ggtacaccag gaagaatatt ggaattgatg
    31201 aaagcaaaaa aaataaaagc acatcaagtg aaaacaatgg tatttgatga agcagaccag
    31261 ctatttgacg ctgggaacag ccaaatcatt gatcaaattc ttcatcaagc tccaacagag
    31321 tatcagctag cttttttttc agcgacagca gatcgttccc ttgaaagcat tgaaaaaatc
    31381 actgggaaaa cgtttgagac aattgacgtg acagcagagg acgattctcg aaaaggactg
    31441 agacattact ttttacgtgt accgatacga aagaaagatg actacttgag acgtctcacg
    31501 catatagaag attttcatgg tctgattttc tttaatcaac tgactgaatt aggcgtaatg
    31561 gaagaaaaat tgctgtatag aggtgtaccg gtaggaagtc tggcctctga tcagaacaag
    31621 ctactccgaa aagcagcgct agaacaattc aaaaagaaaa tggtcagagc acttttgacc
    31681 acagatgtgg cggctagagg tttagatatt acaggtcttc cttatgtagt gaatgcagaa
    31741 gttccgttaa gtgaagaagc ttatttgcat cgttcgggaa gaactggtcg aatgggtaat
    31801 gaaggaatag taattacact cgtacaagat aataatctaa gagatttaaa acggctttta
    31861 cgtccatcaa atataacact tgaagagatt tttttatacg gaggtaagct gcaaactgag
    31921 cagccaacca aagaagatgc agctcctgca gtaaataaaa agtatgcgaa aactttttct
    31981 ggaaaggagc agaacccaga atataacaaa gaaaaaataa gaaaaaaagt taaaaataaa
    32041 tcaaaaaaag agagaaataa aggagcaagg cgcaaataag cgccttttta tgctatgatt
    32101 atgaaaacgg gaacaaaatt ggttgccaga ggaggggtta tgtgaaagaa cgtcaagaag
    32161 agatcttgca gctattgaca gaaaaccaac aaggattgac agcaagtgat gtagctgagc
    32221 gcttaactat cgatcgaagc aatgcaagcc gatatttaag tgaactatac aaggcccatc
    32281 atattgtaaa aaccgctggc cgaccagtcg tttactcttt gccaacggaa aagtcaaaat
    32341 ctgatgaagt gcatgtcgac tcttctacgc aagtgacttt tgaaacactc gttggagaaa
    32401 atgattcttt aaaagtcagc atacagcagg caaaagctgc gattctttat ccaccgcggg
    32461 ggttgcatac gattattttt ggagaaactg gtacagggaa gtcgatgttt gctgaatgta
    32521 tgtatcattt tgcaatcgat tcagagatgc tttctgcaga tgcgccattt gtttctttca
    32581 attgtgcgga ttatgctcaa aatccgcaac tgctctttgg acacatcttt gggatcaaaa
    32641 aaggggctta cacaggagca gctcaagata gtcccggtct aatagccaaa gcagacggcg
    32701 gtatattgtt tttagatgag atccaccgtt taccgccgga agggcaggaa atgctgttta
    32761 ctttcattga taaaggtatt taccgtccgc ttggagaaag tgcacaagta catgaagcat
    32821 ctgttcagat cattggggcg acgactgaat cttcagaaag tttcttatct acgttcaatc
    32881 gacggattcc tatggcaatc accttgccga atcttgcttc acgttcactg gatgagcgtt
    32941 atgaaatcat ctctcttttt atcaaacaag aagcgaatcg tttgaatcaa cgtatcgata
    33001 tcgaaaaaga agcgattctt gccttcatgc tttatgatgc ggaaggaaat atcggtcaag
    33061 tgaaacgaga cttgaaacta gtctgtgcaa aatctttttt acattaccgt actcatcatg
    33121 agaaaaagct aatcattcga aaagaagaac tttcccttca agtacaaaaa ggattactga
    33181 aaatcaagga aataccggaa cgtctcgacc gattcatgga cagcaaaagt cagtatttga
    33241 catttgaacc aggatttgct gatgttgtct ggtcacaaga tccagaacga aacatgcagg
    33301 tctataacga tattgaggaa aaagtcactt ctctttccga aaccggtgtg gagaacattg
    33361 atctagagac cctcatttca aaagatgtgg atgcttattt ccagacatat gtcaaagaat
    33421 tgactaaaag ttcgattccc aaagatcttt tgccagatga aatctggcgg ttaaccaatc
    33481 aattgtatga tgtggctgaa aaacggttag accgtattta taatgaaaaa gctcgttttg
    33541 cttttgcttt gcatctgcaa agtacattgg atcgtgtgag agaaggtcat atgatcgtcc
    33601 acccagattt gaataacgta cggaaaaatt ttaagaggga attccaggta gcaatcgatc
    33661 tttcaacgat cattgaagaa gaagaacaag ttgagattcc ttttgatgaa atcggcttta
    33721 ttagtatgtt tctttctatc aatctaggag aaagtgagcc tgttaaagaa aacaaagtag
    33781 atgttctcat attgatgcac ggcagacaga ctgcttcaag catgttggag atggcacaag
    33841 aactattagg aacaaaagta gggaaagctt ttaatatgcc actggttatg gaagttcaaa
    33901 caatgtatga agaagtgtta caatatgtca aaaactatca agagcatttg acgaatggcc
    33961 tactcttgtt aacagatatg ggatcattaa acaccttcag tgatttgatt tatgaagaga
    34021 ctggcattcg gacgaaagct atcacgatga caagtaccat gattgtgata gaagcgatac
    34081 ggatggcaag tgttggaaga agcttagaag atatttatca aaatatccag ctttcctttg
    34141 aatcgatcgt acgtgatcag ttcaaagcgg aaaagtctga aactttacag cgtaaaaaag
    34201 ccgtaatcgt gacatgcttt actggtgaag gagtagctgc caaattatat cagcgtatta
    34261 tgccagtgat taatcaaaat aaggtagaac tgatccagat gcagttttta gaacgtgaat
    34321 cattcaaaaa acacatcgat gccttattgg aagaatatga gatcaaagca attgcgggaa
    34381 cagttgatgt tgattatcaa aatatcccat ttttttcagc ctatgacgtt tttgatgatg
    34441 aaaaactaaa tattttaaag cgtatagctg atgatgagat cccgacagct aaaattgtcg
    34501 attcacttgc tggatcatta agcgatgtct tttctattga tgaactgatc cgttcattgc
    34561 aaaagacagt tcagcagatc cagaccgatt tgtcgattat cgtggaacct ggagtagata
    34621 ctggaatcgt tatccattta gccttcatgt tagacggatt gatcaaacaa gaaagccatc
    34681 ggcaatttga gaaactgtct attttctcaa aaatttatcg tttggaaatg gatatcgttc
    34741 gggcaaactt gatggcacta gaacgaaaat atcaggtaaa aattccagaa gatgatatcg
    34801 catttcttac acaaatgttt cttgaaaata aaataaaaaa atgaacacac taatcatact
    34861 gtttacacac taaacatctg tgtgcgcact ataaaaaaga gctaaaaagc atgaaaacgc
    34921 tatttcagct ctttttttta ttggcatgct tcttgcgtta tataaaagtg taaaaaaata
    34981 aaaggagtgt taaaaatggc aaaaaaaaca atcatgttag tttgttcagc agggatgagt
    35041 acgagtttat tggtcacaaa aatgcaaaaa gcagcagaag aaaaagggat ggaagccgat
    35101 atttttgcgg tctctgcctc agatgcagat aataatttag aatctaagaa tgtcgatgta
    35161 ttattattag gtcctcaagt acgtttcatg aaagctcaat ttgaacaaaa acttgcgcct
    35221 aaaggtattc cattagatgt catcaatatg caagactatg gcatgatgaa cggagaaaaa
    35281 gttcttgcac aagcagaaaa tctaatgaaa taaatcaagt atgcagttgg gggtataaca
    35341 acgtggaaga tcaacaaaat ctagaagcca tcatggggtt aatcatgttt ggcggtaatg
    35401 caaagagcga tgcaatggaa gcgattgcgg ctgctaaaat cggtgatttt gagttagcgg
    35461 ataggaaaat cgctgatgca gaagaatcac ttatacaagc gcatcatgca caaacagaca
    35521 tgttgacaca agaagcacaa ggaaatcata tgcaagtgac attattgact gttcacagtc
    35581 aagatcactt aatgacatct attgcgttta ctgatttagc aaaagaaatc atcgaacttt
    35641 atcgtcgcgt atacagcgaa taaatacaac ttcttttttg aacggaagaa gaaaaatttt
    35701 tgaacggatt ttttattttg ttataaggag aactctcttt atataaaaaa cttataggag
    35761 gaagtaaaaa atgaacggac tatcaagttg gatggagaaa tacatcctgc cggttgccgc
    35821 aaaaatcgga gcgcaaaaac atttagttgc tttgcgtgat gcttttatcg gcatactgcc
    35881 cgcaacaatg gcgggatcag tcgctgtaat gatcaatgca attattcgtg atttaccgca
    35941 acagtttaac agtacctatg ctgacagttt agcagcagac aaaggaatct ttgccgtatt
    36001 gaatgtcatc atcggaatca atggttttgt ctggaacggt acactagcaa tcgccggatt
    36061 gatctttgcc ttttcttggg ggtataatct tgcccgagct tatggagtaa atgatctagc
    36121 tggggggatc gttggtttag ctaccttgat tcaaggtatt tcctttggtc cagcattatc
    36181 agcagcatta gatgtgaatg ttccttcaaa tgtcgcagat gcaatcaata gttctgagat
    36241 tggtgcaacg attgcagatg gcacactgtc agttggatta tggggttggc taaaactaga
    36301 tcatttgaat ggaaacgctt attttacagt tatgatcatg ggagctattt cagtcattat
    36361 tttttgtaaa ttgatgcttg ccaacattac gatcaaaatg cctgattcag taccaccagc
    36421 agtatctaaa gcatttgcag ccattttacc agcgacgatc gctttatacg tcattgctat
    36481 tatcaattat atcgtaggtc gtgtagctga tgggcaactg atcattgatt tggtgcaaaa
    36541 atatattgca gaaccattcc tagggttatc tcaagggttg ggagcagtat tgatcgtcac
    36601 agtatttgtt caagtattct ggttctttgg tattcatgga tcaaatgtat tggcaccagt
    36661 gctagaggga atctggggac aagcacaact aatcaatatt gatattttcc aaaaaggtta
    36721 taacggaaaa actggtacac cagccgtgtt agatgcaatc gaagatggaa aagcttatat
    36781 gtgggtccgc ggatcgttcg atgcttttgc atggtttggc ggatcaggcg gaacgatcgt
    36841 cttactgata gctatcttga ttttctctaa acgtgcagat tatctaacag tagctaaatt
    36901 atccattggg cctggtatct tcaatatcaa cgaaccagtg atgtttggtc tgccaatcgt
    36961 attgaatgca atcatgttta tcccattctt gattgctcca gtagtagcaa cagcaattgg
    37021 atggacagct acttatcttg gcctagtcgc tccggtatcg cagcaagtca catgggtagt
    37081 accgccgttc ttattgtcat tcttagcaac aggagcggat tggcgggcac cgatcgtaac
    37141 attagtatgt atggtcgtga ctttcttgat ttgggcacca tttgtcatct ctgcaaacaa
    37201 aatggaccct gccgcaggcg aacaacaata agatgaacgt gaggagcggg acgctttaag
    37261 tttcgttcct cataatgaat tttcgaggag gaactgaaat gattacaatt gccagtgcaa
    37321 tgcaacaaga tgaaatcaaa gaattgttag aaggatacaa tgaagaagga atgactttta
    37381 catttaaaga aaaacaagga atcaagctgt tctttgaaac aagtgcagaa gatccggaag
    37441 ttgcggctaa aaaagcaaag gaactaatca aagcacagcc ttggggaaca gtcctttatt
    37501 ttcaatcaac agctgtttag aaaatggttt aaaaagatag gatggttgag atgataaaaa
    37561 acggtttatt tttatgtagt attggttctt tgatcgtgtt gtatagtttg aatccacatt
    37621 cgcaaaggta tgatttgtat agtatggtca caggtctttt tctaacagga ttaggaatct
    37681 atttcttcat gaaggggaaa aaaagagaag aagcaaagga gaaaaataac taatggaaat
    37741 cacgaaaaaa ttaaatattc cagcatcgtt tttttacgat aaagttatgg actctgtcct
    37801 tttcgatgta cgtaaagcga ccggcaaatc agtaactaga aaacagctga aaaatttgga
    37861 gtacgtcaaa caattctcgc aaaataaccg ggctcgcata aaaatcgaag aagtcattga
    37921 aaaccagtct tataagtttc gtacatcgac aacgaagaat gaattcgtcg ttcattatca
    37981 aatcaaacca ttggacgatt cgacctgtga ggttcattac acagaaaaaa tggagtctta
    38041 tggcatgctt caaaagttga atgatatgct tttaggaaca atcttgatgt attttaaacg
    38101 gagacgtttc aaaaaaatgc tagatatgat cgaagcaacg tattaaatca atgaaacatg
    38161 gtactattta gttattgaaa aatgaagtaa gctagaagga atttttcata ggaagataga
    38221 tctttcctgc gaatcctcac tagctttttt gtaggaaggg gcagcaaaat gacaaacaaa
    38281 gtaagagagc taggtgtatc ttcgattgag gaaatgtttg atttagccac ttatgcattt
    38341 aatggggcag atacacctga aaggcgagaa cgttttcgta cacttgcgga gaattcttgg
    38401 aattacggat tttttgatga ggaggatcgg ctaacaagtc aagtcatggc gacccctttt
    38461 ccagtgaatt tttacggaca agagtaccta atggccggca ttggctatgt tgcttcttat
    38521 ccagaagcaa ggggacaagg cgggatcaat cagatcatgg aaaagatcct tgaagattgc
    38581 cgtgatcgta aagtaagctt atcttatctg gcgccattct cctatccctt ctatcggcgc
    38641 tatggctatg aacaatcatt tgataaaatc agttatcaac taaacagtcg tgatgtgccg
    38701 tttatcaaaa aaaaatctgg aaaaataaag cgtatggatt ttgaagatgc aaaagctgca
    38761 atcagaagaa tttatgaaca gatgccggaa aaccaacgag ctggtctgaa gcgcgaagac
    38821 tggtggtata cgtataaatt caagcaaaaa ggcaattacc agtttgcttt gcattttgat
    38881 gataatgagg aagcaacggg atatatcgtt tatcagcttg caacgtcttc ctttattatt
    38941 gcggagtggg gatttttgga tcatgatgct ttttgcagcc ttgttcgctt cgtaagttct
    39001 cacaatggtg cttttgaaac atttgaatat tcatgcggct acggcggtaa tgatcaaaat
    39061 tatctattag aaaatccatt tgcttctgta atgatcacac cttatatgat ggcgaggatc
    39121 gtagatattt ctttgttttt ggaaaattat ccatttaaaa agagacaagc tgcatttgcc
    39181 ctacaaatag aagaagatca ttatgcgaaa tggaatgaag gaatcgtgga agtaacaatc
    39241 gaaaatggaa aaaatcatct ctcaaaagta gaacaaacaa aactcccaat cgttcgagga
    39301 agtatccaac atatcacgca gctattatta gggtatcgta aagtggaaga gttggtattc
    39361 caagaaagaa tacaggtgac aaaagaatat gaagaaacgt taagctcaat cttgccacag
    39421 caacagccaa ttcttaatga ctatttttga taaaaaacgg gtgatgacaa tatttttgtc
    39481 accacccttt tgtttttcat ttggcatttg aaggaaaatg ttaaagtaaa tcctcttttt
    39541 tcgtaaattt gaattttttg ttttcttttg ttttagggtt ttcttttttg tggtcttgga
    39601 ttgcttcttt cgtaatcttt tcccgatttt ttttagatga atctaagcgt tctgcgtata
    39661 ctttttcttt tggcttatca ttcattggaa aggacctccc tacattattt cattctacct
    39721 ctagtttatc atctgttaag gactagtgga aacgttttgc ctaacttata agctgatttt
    39781 tttagcagat aagcttgcaa gtaaaaaaca aacaagctac aattgattta ttgaaattgt
    39841 tcctgtagct cagtaggata gagcaatcgc ctcctaagcg ataggtcgct ggttcaattc
    39901 cagtcaggaa cgtataaata acccctgtat cctttgcggt acaggggttt gttttgtatt
    39961 tgtgccaaaa atgtgccaaa ctagttatca tttttatata aactatcgaa cacagtattg
    40021 attttttctt tatcctgatc ttcaagctct cgaacaatat gaagataagt agacattgta
    40081 gtttctaaac tgctatgtcc taaacgtttc gatacgctaa gtatatttac tccttgataa
    40141 agtaaaatag atgcatgtgt atgccttagt ccatgcaaag taaattgctt ttcaatccct
    40201 aattctttta attttttgcg caaatatttg ctgacggcgt ttgatgaaac taatccattt
    40261 tttaagttga aaaaaacaaa attatgaggg tttttgattt caaaattctc gaataattct
    40321 ttttgattga ttttaaactt ttttaagaga tcaatcgtat gtttatcaat ggaaattttc
    40381 ctatttgaag tttcgttttt tgtttttccc cattcattta atttataatt ccatgtttta
    40441 ttaatagtga tgatttgatc ttcgaaatcg atatcttccc atgttaatcc tagaagttca
    40501 gcaaatcgca ttccagtggc accagcaact aacacgagca taggagaaga gtactgcgca
    40561 gaaaggtttt cttctacgag tttcattaaa gatttaaatt gatcataatc taaatattta
    40621 tcttcttttg gcttcaatga atcttttcct ttgattactg cttttctagt tggatcaaac
    40681 ggaattaatc cttcttctac ggcatctttc aacgacgctc taatatggtt attaaactta
    40741 ataacggtag attttacatg gtctttagca tacttattta aaaagcgttg atatcccact
    40801 ctatctaaat cagaaatcaa taccgctggc atatatttct ttatgttcat taacgtatct
    40861 tcgtattttc tataagtgat aggagatact gttccttctt tataaagttg catccaatcc
    40921 tcaaagtaat ctgataataa tagatttttg cgatcccctt taaggccttt gttcaagtta
    40981 tattctaatt cgttggcggc atcttttgct tctccttttg tcttaaatcc tgattttctt
    41041 atcttcgtgt attttccgtc atcttttttg tatgaaattt cgtattgcca actatttcca
    41101 cgtttgacta atcttgccat aattgaatca gctctctttc tctgatacaa taggaactat
    41161 aaagaagccc attgtttggg ttttattatt ttttgcacat cctttttgct tgccggcgca
    41221 aggatgtgct atttgtcgtt ttaatggtat aattggatgt gtattagatc atccaatcag
    41281 aggtgtttat tatgattaaa gtgattttta aaagaataac tgaacttcca gagtttagat
    41341 tatttgttga ttattggtat gtctggtttt ttattgttgc attgctgata ggtctagatg
    41401 tcatattaga aagaaaactt aagtaagagt tgagttaata aattgaattc ctaaatgaga
    41461 tattttgtag cccatatttt gtttgccgac atcacctaat actatggagc taatcgtttt
    41521 ttctaacaga ccaaaatcag ataatctatt taagctacca tctattagct cttgtgaata
    41581 gccagacatg ctgatatttc cagtgaaatt ttcgtttcca cgactcatta agcatgctaa
    41641 caattgaatc tcaatcgatg ttaattgatt cagtacaaga agataatact cctcgttatc
    41701 ccatgaagcg ttgttaattc gaagtaaaca attcttataa agatttttat acatatctat
    41761 tttattttgt gcctttgatt tttctacctc gtcatttatc ccttcgaata ttccaatgag
    41821 ttggtccttg ttctctaagt tgaaaacatc ttctggaatt tgatcgcgaa tgttttctaa
    41881 tgattcgttt aaatttttgt agaatttctc gatgcgtttg aaacgcttct cgtttttaga
    41941 cccaaaataa agtgactgca caacgccacc aatataagga attgctccaa taccgccttc
    42001 aactattaat tcaagatatt cttttttggt tagtttttca ttgctattat ccatagttac
    42061 ctcctatttt gtcatttcct ttacaagcat tgtgactcct gatggcactc ttttgtatag
    42121 gttttattat tgtgcacaat ctaactctta gtggggcgtg gttcatttat tttttactta
    42181 ctttactatt aatcatatcc cattgagcat aggatttttc tatgttcgat cctcttgaac
    42241 taatttctcc tgtaaaagtt actttatcgc cctttgtaaa ggtatcagca ttgtttaagt
    42301 ccttcgcaaa aattacatat gaaactttag gtgttgcaga aatatcattc caacttttat
    42361 catcgtactc tttgtctgct ataatggcta ttctagtagt cattgattcg ataacagttc
    42421 cttcaaaagt atatgtttta ccactaatat atttagtaaa agctgtattt tgctcagtag
    42481 ctggagtaat agaataataa ctatctaaga aagcacccca gtcatgatta tttgtctcta
    42541 aaaattcttc aaattgagtt aggcttttat tgctgctgtt agatgtagta gtttgagtat
    42601 cagatgatga gctttcctgt ttgttattgt catttgcctc gtttgatgag gaaagcgaga
    42661 aacctacggt agtaaaaata atcccgataa taaggagaat cataggtgtt ttcttatttg
    42721 tttttttgat aaatcttaca attaacatga ttaaacctat aataaataaa acaataccag
    42781 taaaaccaaa tataccgtcc attaatagat cctctttctc ttttgatatt gaatattata
    42841 aaccaagcaa ttcttttttc ttaaaatcga attcttcttg attaataatc ccttcatcta
    42901 acaactcttt atacttttta atttcatctg cacttgaaat ttgtgtttct ttggaattat
    42961 tatctaatat agattgttgc ttctctatat atttttttaa attttccatt tcattccaat
    43021 atttttttgt aaacataacg gtgttttcat ctttcgtggc cgctaatata cctccgcgac
    43081 tttcgtttcc gcctagcaag gtaaattgta tataaccatt actcatacct ggttttttta
    43141 attgtacggc tgatatattt ttaaagggga tagttttttc tcctttaata ccttgattaa
    43201 ctaaatttat aaatcctttt ctaataattg aaatagaatc atcagttact ttaatcaagg
    43261 tttttcctgg agattttatt aaaatctctt tttccatttt ttcttcctca tttctgtgat
    43321 atgatttttt ttgtagaagc tcatatatga ggaaagagcc cgtgtt
//
LOCUS       DAYYGO010000007        43364 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000007 DAYYGO010000000
VERSION     DAYYGO010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.007"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            62..2659
                     /gene="adhE"
                     /locus_tag="ACHA5T_000356"
                     /gene_synonym="adhC"
     CDS             62..2659
                     /gene="adhE"
                     /locus_tag="ACHA5T_000356"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFM8885963.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            2888..3994
                     /locus_tag="ACHA5T_000357"
     CDS             2888..3994
                     /locus_tag="ACHA5T_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8885964.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            4011..5039
                     /locus_tag="ACHA5T_000358"
     CDS             4011..5039
                     /locus_tag="ACHA5T_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFM8885965.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLEGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            5045..5524
                     /locus_tag="ACHA5T_000359"
     CDS             5045..5524
                     /locus_tag="ACHA5T_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFM8885966.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            5540..6835
                     /locus_tag="ACHA5T_000360"
     CDS             5540..6835
                     /locus_tag="ACHA5T_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFM8885967.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            6846..7670
                     /locus_tag="ACHA5T_000361"
     CDS             6846..7670
                     /locus_tag="ACHA5T_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM8885968.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPETP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            7835..8734
                     /locus_tag="ACHA5T_000362"
     CDS             7835..8734
                     /locus_tag="ACHA5T_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8885969.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(8860..9729)
                     /locus_tag="ACHA5T_000363"
     CDS             complement(8860..9729)
                     /locus_tag="ACHA5T_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFM8885970.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            9882..11342
                     /gene="rhaB"
                     /locus_tag="ACHA5T_000364"
     CDS             9882..11342
                     /gene="rhaB"
                     /locus_tag="ACHA5T_000364"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFM8885971.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            11339..12595
                     /gene="rhaA"
                     /locus_tag="ACHA5T_000365"
     CDS             11339..12595
                     /gene="rhaA"
                     /locus_tag="ACHA5T_000365"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFM8885972.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            12609..13454
                     /gene="rhaD"
                     /locus_tag="ACHA5T_000366"
     CDS             12609..13454
                     /gene="rhaD"
                     /locus_tag="ACHA5T_000366"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFM8885973.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            13456..13767
                     /gene="rhaM"
                     /locus_tag="ACHA5T_000367"
     CDS             13456..13767
                     /gene="rhaM"
                     /locus_tag="ACHA5T_000367"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFM8885974.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            13849..15000
                     /gene="fucO"
                     /locus_tag="ACHA5T_000368"
     CDS             13849..15000
                     /gene="fucO"
                     /locus_tag="ACHA5T_000368"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFM8885975.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEVSVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            15127..15885
                     /locus_tag="ACHA5T_000369"
     CDS             15127..15885
                     /locus_tag="ACHA5T_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFM8885976.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIQTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            15985..16629
                     /locus_tag="ACHA5T_000370"
     CDS             15985..16629
                     /locus_tag="ACHA5T_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFM8885977.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            16636..17643
                     /locus_tag="ACHA5T_000371"
     CDS             16636..17643
                     /locus_tag="ACHA5T_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFM8885978.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            17659..18501
                     /gene="kduI"
                     /locus_tag="ACHA5T_000372"
     CDS             17659..18501
                     /gene="kduI"
                     /locus_tag="ACHA5T_000372"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFM8885979.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            18521..19324
                     /locus_tag="ACHA5T_000373"
     CDS             18521..19324
                     /locus_tag="ACHA5T_000373"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFM8885980.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(19573..20637)
                     /gene="fni"
                     /locus_tag="ACHA5T_000374"
     CDS             complement(19573..20637)
                     /gene="fni"
                     /locus_tag="ACHA5T_000374"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFM8885981.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(20634..21719)
                     /locus_tag="ACHA5T_000375"
     CDS             complement(20634..21719)
                     /locus_tag="ACHA5T_000375"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFM8885982.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(21732..22709)
                     /gene="mvaD"
                     /locus_tag="ACHA5T_000376"
     CDS             complement(21732..22709)
                     /gene="mvaD"
                     /locus_tag="ACHA5T_000376"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFM8885983.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(22702..23646)
                     /gene="mvk"
                     /locus_tag="ACHA5T_000377"
     CDS             complement(22702..23646)
                     /gene="mvk"
                     /locus_tag="ACHA5T_000377"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFM8885984.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            23962..24621
                     /locus_tag="ACHA5T_000378"
     CDS             23962..24621
                     /locus_tag="ACHA5T_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM8885985.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            24706..25611
                     /locus_tag="ACHA5T_000379"
     CDS             24706..25611
                     /locus_tag="ACHA5T_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM8885986.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(25613..26245)
                     /locus_tag="ACHA5T_000380"
     CDS             complement(25613..26245)
                     /locus_tag="ACHA5T_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFM8885987.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     misc_feature    26396..26521
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            26565..27089
                     /gene="infC"
                     /locus_tag="ACHA5T_000381"
     CDS             26565..27089
                     /gene="infC"
                     /locus_tag="ACHA5T_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFM8885988.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            27161..27361
                     /gene="rpmI"
                     /locus_tag="ACHA5T_000382"
     CDS             27161..27361
                     /gene="rpmI"
                     /locus_tag="ACHA5T_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFM8885989.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            27414..27773
                     /gene="rplT"
                     /locus_tag="ACHA5T_000383"
     CDS             27414..27773
                     /gene="rplT"
                     /locus_tag="ACHA5T_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFM8885990.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            28025..29362
                     /locus_tag="ACHA5T_000384"
     CDS             28025..29362
                     /locus_tag="ACHA5T_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFM8885991.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            29410..31539
                     /locus_tag="ACHA5T_000385"
     CDS             29410..31539
                     /locus_tag="ACHA5T_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFM8885992.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            31514..32203
                     /locus_tag="ACHA5T_000386"
     CDS             31514..32203
                     /locus_tag="ACHA5T_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8885993.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(32611..33224)
                     /locus_tag="ACHA5T_000387"
                     /pseudo
     CDS             complement(32611..33224)
                     /locus_tag="ACHA5T_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(33360..33554)
                     /locus_tag="ACHA5T_000388"
     CDS             complement(33360..33554)
                     /locus_tag="ACHA5T_000388"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8885994.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(33604..34044)
                     /locus_tag="ACHA5T_000389"
     CDS             complement(33604..34044)
                     /locus_tag="ACHA5T_000389"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFM8885995.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(34048..34893)
                     /locus_tag="ACHA5T_000390"
     CDS             complement(34048..34893)
                     /locus_tag="ACHA5T_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFM8885996.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            35042..36460
                     /gene="pepV"
                     /locus_tag="ACHA5T_000391"
     CDS             35042..36460
                     /gene="pepV"
                     /locus_tag="ACHA5T_000391"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFM8885997.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(36517..37464)
                     /locus_tag="ACHA5T_000392"
     CDS             complement(36517..37464)
                     /locus_tag="ACHA5T_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8885998.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(37687..38037)
                     /locus_tag="ACHA5T_000393"
     CDS             complement(37687..38037)
                     /locus_tag="ACHA5T_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFM8885999.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            38237..39316
                     /gene="pepA"
                     /locus_tag="ACHA5T_000394"
     CDS             38237..39316
                     /gene="pepA"
                     /locus_tag="ACHA5T_000394"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFM8886000.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            39460..39780
                     /locus_tag="ACHA5T_000395"
     CDS             39460..39780
                     /locus_tag="ACHA5T_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFM8886001.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            39802..40266
                     /locus_tag="ACHA5T_000396"
     CDS             39802..40266
                     /locus_tag="ACHA5T_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8886002.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            40471..41076
                     /gene="ytpR"
                     /locus_tag="ACHA5T_000397"
     CDS             40471..41076
                     /gene="ytpR"
                     /locus_tag="ACHA5T_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFM8886003.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(41115..42404)
                     /locus_tag="ACHA5T_000398"
     CDS             complement(41115..42404)
                     /locus_tag="ACHA5T_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFM8886004.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            42832..43311
                     /gene="rlmH"
                     /locus_tag="ACHA5T_000399"
     CDS             42832..43311
                     /gene="rlmH"
                     /locus_tag="ACHA5T_000399"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFM8886005.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
BASE COUNT        13700 a         7928 c         9301 g        12435 t
ORIGIN      
        1 tattgtatga tatacatgtg aattatttaa caaataacca tgacccatta ggaggataat
       61 gatggctaaa gtaatagaaa aagaaaaaac gaagacaatt gacgtacaag caatgattga
      121 tgatttggca gaaaaagcaa atgcagcatt gaaagaaatg gaaagctttg atcaagaaaa
      181 agtagaccat atcgtgcatg aaatggcaat ggcagctttg gatcaacata tgccattagc
      241 gaagatggct gtagaggaaa caggccgcgg tgtttacgaa gataaagcaa tcaaaaatat
      301 gtatgcgtcc gaatatattt ggcataatat caaacatgat aaaacagttg gtgtgatcaa
      361 tgaagatgtc caaaaagggt tgattgaaat tgctgaacca gtcggtgtgg tttgcggagt
      421 aactccaaca acgaatccga cttccacaac gattttcaaa tcaatgatcg cgttgaaaac
      481 aagaaatcca atcgtttttg ctttccatcc aagtgcccaa aaatcttctg cagaagcagc
      541 acgagtggta cgtgatgctg ctatcgctgc cggagctcct gaaaattgta tccaatggat
      601 cgaacatcct tcgatcgaag cgacttctat gctaatgaac catccaggta tcgcgatcgt
      661 cttagcgaca ggtggagctg gaatggtcaa gtctgcttac tctacaggaa aaccagcttt
      721 aggtgtaggt ccaggtaacg taccagctta tattgaaaaa acagcaaaaa ttaaacgtgc
      781 agtgaacgat ttgatcgttt cgaaaacatt tgataatgga atgatttgtg cttctgaaca
      841 agctgtgatc gtggataaag aaatctatgc agcagtaaaa gcagaattcc aagcacacca
      901 agtttatatc gtaaaaccag atgaattgca aaaattagaa gatgcagtga tgaacgaagg
      961 aaaatatgcg gtcaatccat caatcgttgg acattctgcg atggaaatcg caaaattggc
     1021 aggaatcagt gtaccaaaag gaacgaaaat gctgattgct gaattggaag gcgtaggccc
     1081 agattatccg ctatcaagag aaaaactttc tccagttctt gccatgatca aagctaacaa
     1141 cacggaccat gcgtttgatt tgtgtgaagg tatgttgaat cttggaggat taggacatac
     1201 agcagttatc cattcagaaa acgaagagtt gcatgtcaaa ttcggtcttc gaatgaaagc
     1261 ttgtcgtatt ttagtgaata caccatctgc agaaggcggc atcggtgata tctataacga
     1321 aatgattccg tcattgactt tgggatgcgg ttcttatggt aagaactctg tttctcgtaa
     1381 cgtctctgct gtaaacttaa tcaatgtcaa aactgtagcg aaacggagaa ataacatgca
     1441 atggttcaaa ttaccgccaa aaatcttctt tgaaaagaac tctctattgt acttagagaa
     1501 aatggaaaac gttgaacgtg tcatgatcgt ttgtgaccct ggtatggtcc aattcggtta
     1561 ctgcgataca gttcgtgaag tattggcacg acgtaaaaat gatgtgaaga tcgaagtctt
     1621 ctcagatgtt gaaccgaacc catcgacaaa taccgtatac gcaggaacga aactgatggc
     1681 agacttcaag ccagatacag taatcgctct tggaggcggt tctgcaatgg acgcagctaa
     1741 gggaatgtgg atgttctatg aacacccaga cacatctttc tttggtgcaa aacaaaaatt
     1801 cttggatatc cgtaaacgga catataagat cgacaaacca gaaaaaacac aattcgtatg
     1861 tatcccgaca acatcaggga ctggttctga agtaacgcca tttgcggtca tcactgacag
     1921 tgaaacacat gtgaaatatc cgttagcaga ttatgcattg acgccagatg tcgcaatcgt
     1981 cgatcctcaa ttcgtcatgt cagttccagc atctgtcaca gcagataccg ggatggatgt
     2041 gttaacacat gcaatcgaat cctatgtttc agtcatggct tcagattata cacgtggatt
     2101 gagcctacaa gcaatcaagt tagtctttga ctatttagaa aaatcagtaa aaacacctga
     2161 tatggaatca cgtgaaaaaa tgcacaacgc ttctacaatg gctggtatgg cgtttgctaa
     2221 cgcattccta ggtatttgtc actcagtcgc acataaaatc ggcggggaat acgggatccc
     2281 tcatggacgg acaaatgcga ttcttttacc gcatattatc cgctataatg caaaagatcc
     2341 acaaaaacat gcgatgttcc ctaaatatga ctatttccgt gcagatacag attacgcaga
     2401 tatcgcaaaa ttcttaggtc tgaaaggcga aacaacagag gaattagtcg aagctttagc
     2461 aacggctgtt tatgaattag gaaaatctgt cggcatcaac atgagtctaa aagctcaagg
     2521 agtcacacaa gaaacactag atacaaccgt agaccgtatg gctgaattag cttacgaaga
     2581 ccaatgtacg acagctaatc cgaaagaacc attgatctct gaattgaaac agatcatcat
     2641 tgatgcttac aacggataag caaaagaaag cgtagaaaaa agataagaaa atagatgaaa
     2701 aagaacttgg aaaacgttgt ttctccaagt tcttttttta atttccaagt aactgaaagt
     2761 tacttttgtt atttaacatt taggtcaatt ttagacagtt tttgttcaaa aagcgatatt
     2821 tatagtggac ggtattactg ctatattaag gatataaaat gaaagcgttt aaaaaggtgg
     2881 cgtaaagatg attattaaag aagagatttt tgtacctaaa gaattagtga aagaaaaaat
     2941 tgacctgctc gttgaaaacc tgacaacgat caaagacgat aatggtgaat ttttgttgga
     3001 ctttgatgga ctgaaagtag atgacaaaag ctggacagtg tggaattggc cacaaggtgt
     3061 cggtctatat ggtatttata aaaattacca gatgacaaaa aatcctcgcg cttatcaagt
     3121 ggtcaatgaa tggtttgagg atcgtatgga agaaggcgct ccgcccaaaa acgtcaatac
     3181 aatggcaccg ctattgacta tggcttactt gtatgaagac acgaaagatt cacgctattt
     3241 gccttattta gaacaatggg cagaatgggt catgaatgac atgcctcgga caaatgaaga
     3301 cgggttacag catgccacat atggaccgga aaataagaat cagttgtggg acgatacatt
     3361 gatgatgaca gtgcttcctt tggcaaaaat cgggaaactg ttgaatcgcc cagaatattt
     3421 agaagaagct cgttaccagt ttatgatcca tatcaaatac ttgatggata aacgaacagg
     3481 attgtggtat cacggctgga cgtttgaagg aaatcataac tatgccgaag cattttgggc
     3541 tcgtggaaac tgctggttga cgattgctat tccagagatc attgaaatct tagaattatc
     3601 gaaagaagat gcattgcgca aattattgat cgaaacactt gaagcacaag tgcgtgcatt
     3661 aaaaacttat cagtctgaaa gcggtttatg gcatacgttg ttggatgatc cttcttctta
     3721 cgtagaatct tctgcaactg caggatttgc ttacggtatt ttgaaagcgg tccataaacg
     3781 ttatttacca caagaatata aagaagtcgc ttatcgtgct atccaaggat tgcttgaaca
     3841 aatcgatgaa aaaggagaag ttcaaaacgt ttcaatcggg acaggaatgg gggacagttt
     3901 agatttttat cggaatatcg gaatcacagc aatgccttat ggacagtcgt taacggtgct
     3961 ttgtttgaca gaattgctcg tctcttattg ttaaaagaaa ggaaacccat atgaaaaaaa
     4021 catcactgct gtttgcagca agttcgctta gcctaggtct gctaggtcta agcggctgcg
     4081 gaaataattc tagtgtggca actgtcgcag atgcagatgt aaatgagata gttaccttgc
     4141 gctttgctta tgcaagcaac agccagccag taattgatgc aatgaatgaa tttgggcgtt
     4201 tagtaaaaga aaaaacagat ggttccgtac agatccagta tttcccagat ggacaactgg
     4261 gtggagaacg tgaactgatc gaattgacac agtcaggagc agtagatttc acaaaagtaa
     4321 gtgcctcagc gttggaaagt ttctctaaag attattcgat tttttcagtt ccttatctat
     4381 tcaatgatga agaacatttc tttaaggtga tggataatga agaaataatg gagccaatct
     4441 accaatcgac caaaaattta ggatttaccg gattgaccta ttatgattct ggtcagcgca
     4501 gcttttatat ggtagacggt ccgatccata cgccggaaga tttggaggga aagaagatcc
     4561 gtgtgatgca aagcgaaacc gctatcaaaa tggtagagct tttaggcggt tcgcccgtac
     4621 ctatgggaag tgatgaagtg tacacgtctc ttcaatccaa tttgatcaac ggatcagaaa
     4681 acaatgaatt tgtcttgtat acagctggac atggtggtgt ggctaaatac tattcctatg
     4741 atgagcatac acgtgtgcca gatatcatca tcatgaatga tgccatcaaa gaacagctaa
     4801 ctgatgagca ggaaaaagcc attgaagaag cagcgaaaga atcaacggtt tttgaaattg
     4861 aagcttttgc aaaagcaatt gaagaagaaa aagctttagc acaagaagaa catggagtcc
     4921 aattcaatga tgtagataat acaccgtttc gagaggcagt tgccccactt catgatatat
     4981 tcaaaaacga tcctacctac aaagaccttt atcaaaagat ccaagagcaa ggagcgtagc
     5041 tgtgatgaaa atgattttaa acaaggtatt agagatcctg ggagctgttt tgcttgttgg
     5101 gatggtcgct gttgttttat ggcaagtatt ttccagaacg gttttaggaa atccaaatac
     5161 ggtcacggaa gagcttgttc gattcggtct tgtctggttt gccatgttgt cttccgcgta
     5221 tgttgtggga caaaaagcac atttagccgt aacggtttta agtgaaaaat taactggcaa
     5281 aaaaagtaaa attttagaag ttgttgttca aagtttattt ctggtatttg ctgcgatgat
     5341 catggtgtat ggcggatgga atgctgtcac attgacaatg gggcagatct cgccttcttt
     5401 aagtattcct atgggatatg tttatctgtc agtacctgta tcgggggtac tcatcattat
     5461 ctatagcctg atcaatctaa tcgatacatt aagcaacaag ccagcaattc aagaaaaaat
     5521 ataaaaatgg aggaataaaa tggcaataga agccggatta gtgttatttt ttgtatttgt
     5581 tgtgttgctt gtattaggga tgccgattgc tataagtatt gcattctcat cgatgatgac
     5641 tttattgtta gttataccgt ttgatgtttc tgcttttagt tcagctcaaa aaatggtggg
     5701 aagtatcaat agtttttcgc tgatcgctat tccgtttttc gttctctcgg gcattatcat
     5761 gaataatgga gggatagcta agaaactagt ggattttgcg atgttgttcg tcgggcgcgt
     5821 gccgggggct ttggctcata cgaatgtgtt agggaatgct ttatttggtt ccatctctag
     5881 ctcggccatt gccgcttcta ccgcgattgg cggagtgttg atccctcagc aagtggaaga
     5941 aggatatgac cggaagtttg cgacagcggt caatatcgcg tcagctccga caggtatggt
     6001 cattcctcca agtactgctt ttatcatgta ttcacttgtt gctggtggtg cttcgatctc
     6061 tgctttgttt atgggaggtt atctcgtagg cgctttgtgg gcgctaggaa tcatgctagt
     6121 agcattcatc gttgctaaaa agaataaata ccctgttgtc gaaaaaggac agatggctca
     6181 tgctggaaaa ataataaaag aggcgattcc tagccttcta ttgattgtca tcattatcgg
     6241 tggtatcttg acggggatat ttacagcagt agaagcttca gcgatagctg tcatttactc
     6301 cctgttgatt gctatgtttt actataaaac ggtaaaacta aaagatatac ctaatatgtt
     6361 gtttcaatca gtagcaatgt cagggacgat catgttctta ttggctactt cttccatgat
     6421 gtctttcgcc atggcgttta ccggtattcc tcaggcaatt agttcgttga ttatgggcgt
     6481 aacggataat aaatttatta ttctgttatt agtcaatctt gtgttattac tggtgggtat
     6541 gtttatggat gtggcgcctg ctatcttgat ttttacacca atatttttac caatcgtcac
     6601 aagtgtaggg gttgatcctg tacattacgg gttgttttca atcatgaatc tgtgtgtagg
     6661 ctccatcacg ccgccagtag gtacaggact atatgtcggt gcaagtgtag gaggcgtaaa
     6721 agcagaggca atgctgaaac cgcttcttcc tttttatggg gttatcttag tcgtcttgtt
     6781 tttgattact tacttcccgc aaatcgtcat gtggctaccc aatatgctga gttaggagaa
     6841 tagttatgtt gaacattaca attagaggac atgatctttc cgaagtccaa acgatagaag
     6901 atcttgcaga aaaaacaaaa gaacaaggga ttcatacact gcagctcgcc ttaggtcttt
     6961 cgtttcctga aacaccttct ggcgcaaatg agatcaattc tgggatggga aattatgtca
     7021 aacgagtttt agaaaagcaa gaagtgaccg taggaatttt aagttgctat atcaatatga
     7081 ttcatccaga tttaacgata cgacaagagt tgctgacaaa gttcgagcag tatcttcgct
     7141 atgcttcttc ctttggtgcg tcgatggttg cttcggaaac tggatgtgtt ttgccggaaa
     7201 tccaatatac ggaagaaaat ttcacagatg aagcatttgc tgaagctgtg tccgttatcc
     7261 gtcgcttggt aaaagctgga gaaaaatacc aaatgatggt ggggatagaa ccaggtttga
     7321 atcatccggt gtattcttta gcaagggtag agcagctgat ccaagcagta gattcggact
     7381 atctaggaat cattttagat ccgaccaatt tgatcacaag tagtgattat caagaacaag
     7441 tccaattggt agaagaagct tttgaacgtt ttggtgaaaa gatatgtgcg gttcatttga
     7501 aagattttcg tgtcgaacag gaaaaaatcg tgccggtaaa tttgggagac ggtgtgatcg
     7561 agtacacaaa aatcaaagaa atcatcatga agaatcgtcc gtacctgtat gtggttcttg
     7621 aagaaacaaa agatgatggg attcgttatg gaagaagtct tcttgaataa tgtaaagaag
     7681 acgttggtta actaatattt agaatggagg gggcggggct agaagatgcc acgtctcttc
     7741 ttttttataa gagtatataa aagcagctct ttttgattac aaaaaacgtg gtataataaa
     7801 attgtgcata agtgaacaaa aggaggtgcg gcacatgctc caaaagaaaa tcagcggtga
     7861 acaagtcaat acgatggttt ctgatcagta tgaaatctat catgtcaaag atgtcgtcag
     7921 ccaaaataaa acgatctatc atcatcatga tttctatgag atccatgcta cattaaaggg
     7981 agaagcgact ttttatttga acggacgaca atttacgatc aaagcaggaa gtgtgctgct
     8041 catccattcc aatgatttgc atcgtatcgt ccgccaatct tcagatgtat tcgagcgtgt
     8101 ctatatcttt gtcacccctt cttttctaga aagtcgttcc acgccatggt ctaatctatc
     8161 ggcttgtttt caaccaatgg gagagaagag gagtcgtgta ttgcaaatag aacaggaagt
     8221 attgaagcaa aaattatcat ttatcgacca tcctcctgat aaaaaaattt acggtgaaga
     8281 cataaaatat gaacaagctc tggtagaata cttgattttt ctcaatcaag ttgtacaaaa
     8341 agaagagagt gcgcaagaac agaatgctgt agttcctaat gagcgtgtgg aacaaatgtt
     8401 acaatatatc tcaaaaaatt tatctcatcc gctttcttta gaggaaatgg aaaagcagtt
     8461 ttttgtcagc aaataccata tcacacgaga atttaaaaag tacactggat ttacgtttca
     8521 tcagtatgta atgaagaaaa agctgatcta tgccaagcac ttgttgcgag aataccgcag
     8581 ctctagtgct gtatatagta aaagtggttt ttcttcttat ccgcattttc tgaaagcatt
     8641 caagagagag ttcggcataa cgcctaaaga atttttgaaa cgtgatacac agaaacagtt
     8701 ggttcattat gaacattttg aagatgaaca atgagaatga tcactaaaaa ataaccgcag
     8761 tagctgctgt tgtttacata gaagcagaaa ctgcggttat tttattttta tctatatatt
     8821 tcaaaatatt ttgaaaaaat gacgtacata ttatatgaat cagctgaatt tacgaaagtc
     8881 tctcggcgat agtcctgttt ttgctttgaa caggcggcta aagtaagcaa aattctcaat
     8941 ccctatttct tcggaaatca ttcgaaccgt aaattctgta taccgaagaa ggtctttagc
     9001 gtaagaaagt tttttgctga tcaaataatc gattggtgga gaaccgataa atttggaaaa
     9061 atctttgctc agctgatatt tattcaataa gaattgttgt tctaattgat caagtgtaat
     9121 cgtttctttg aaatgttcgt ctaagtattg cttcagtttg taaacgtagg cgggaatatc
     9181 ctcttcttcg aaatctagct gatacttttg caaaatcagt tcattcagca actcgtggat
     9241 agctacagag gactggaaat cggtacgggc attcgtgcct tgttgcatgt ggatcaattc
     9301 tgtgatcaaa tggtggatct tgttttctga tggatcccct gttgaatgga aggtgttatg
     9361 gccgtctttt gtgaactcct gataaaatga ttggacgttc tgaccattca aatggatcca
     9421 atccatttcc catggttctt cgctgatcgt ttgataacgc tgatattctt ggcagtcaat
     9481 aaaaaataag tctccagctt ttagctgttg ctgattatca ttatatgtaa gcgaaccttt
     9541 gccagacaaa gtaaacttta tcaagtaaga aggtaaatgt gctcgttcag tatagtaagg
     9601 gattggtgct ttgaagtaac cgatttcttg gatataaaaa aataaattcc tgcttctttg
     9661 tgtaggcgtc aagatcactc tttttgagtc ttccgaccat gagtggttgg ctttttccca
     9721 gtaatccatg taaacactcc caacttgtca agataatgca attatatacc aaaatagtgt
     9781 gaagcaaaat ataagattct gtattatgat agcattgtta ataaatgata acgctttatt
     9841 aataaagtgc taatcaacaa taaagtaaaa gaggtggaga aatgaatcca acatttgttg
     9901 ccatcgatat cggtgcatca agcggacgac taatgaaaag tacgagggat gcaaaaggtt
     9961 ctttgatctt ggaagaagta caccggttca aaaacggctt tcggaacaaa gacggtcacg
    10021 atcgctggaa gatcgattat cttatcaaag agattttgat tggatttgaa aaattaaagc
    10081 agacagggat tgatcgcctt tttgcgggga tcgatacatg ggcagtcgat tactgtctga
    10141 ttgatgaaca aggggatcgg ataaatgatc cgattgctta tcgggacaag cgtaccaatg
    10201 gagcagtaga acagttttct gaaatctatt ctctagataa gttgtatgag cagacaggga
    10261 ttcagatcca gcctttcaat accttgttcc aattgtttgc tgaagaaaaa gaaaatttgg
    10321 caaaagcaca taaattgtta ctgatacctg attatttagg gtatgtcttt actggtaaca
    10381 tggtgacgga gaaaactaat gcatctacta tgcagctgct gaatgcaggg acaaaagaat
    10441 gggaaataga acttcttgaa aaaatcggtg tctcgaagga attatttcca ccattgacag
    10501 agccgggaac gatactagga gatctgcaaa aagaacaatt ttcaaaatat gatttgccag
    10561 atgtgacctt tatcacagta gcaagtcatg atacggcgtc agctgtatta ggaacgccag
    10621 gtcaaggaga acgttggggt tatatcagca gtgggacatg gtcgctttta gggattgaaa
    10681 caaagatcgc caatgtttct cgcaatgctt tttctgaaaa ttatacgaat gaatggggtg
    10741 cccataacac ggtgcgtttt ttgaaaaaca tcatggggat gtggttgatc caggaagttg
    10801 cccgaatgca agagtacgag tacagttatg cggaattggc agagcttgct gcaaaagaaa
    10861 aaccgctgca gcaagtgatg gaaatcaatg accctcgatt tttgaatccc ccaaatatga
    10921 tagaagagat tcagcaatac tgttttgaaa cagagcagcc agtccctgtc tcatcgggag
    10981 ctatcgctcg ttgtgtgtat gataatcttg ctctttgtta cgcacaagag ctgatcaaag
    11041 tagaacactt aactggaacg aaattggaaa agctgcacat cgtagggggc ggatcgaata
    11101 atcgtctact gaatcaattg acagcagatt atgccggaat cattgtagaa gcaggaccta
    11161 gtgaagccac agcaatcggg aatgttgcca tgcaaatgct cagtacaggt cagttttcga
    11221 atatagaaca agcaagaaat gtaatcaaac agtcgtttcc atgtgaaact ttttacccag
    11281 atgaaaataa gcaaaacacg atggaatggt acgtgaataa aatcaaagga gtaatcgtat
    11341 gaacacgatt gaaagaaatt acgaacaagc aaaagaaaaa tatgcaacta tcggtgtaga
    11401 tacagatgct gtactagaaa agatacagga catcaaaatt tctatgcatt gctggcaagg
    11461 agatgatgtc aaaggtttct tgacaccgga tggcgaattg accggtggaa tcatggcgac
    11521 agggaatttt ccaggagccg cacgaacacc tgaagagctt agacaagatc tggagaaggc
    11581 ttattcgttg ataccgggca aacacaaatt gaaccttcat gctatctatt tggatacaga
    11641 agaatctgtc gatctaaatg aaattgagcc gaaacatttt gaaaaatggg tagagtgggc
    11701 aaaaaaagaa gaaatcggtc ttgacttcaa tccgactttc ttttcgcatc caatgatgaa
    11761 agacggcttt acattggctc atccagataa agaagttcgt gaatactgga tcgaacacgg
    11821 gaaaagaagc cgcaaaatcg cggagtactt tgggaaagaa actgggcaaa caagtatcaa
    11881 taacttttgg gtacctgatg ggatgaaaga taatccgatt gatcgtttta ccccacggga
    11941 acgtttaatg gaatcactcg atgaaatttt tacagaagag ttagatgaac ggtatgtaca
    12001 agatgcagta gaaagcaaac tatttggttt aggcgctgaa gcttatacag tcggttccca
    12061 tgaattttat atgggttacg ggttgactcg tgacaaactt gtttgtctag atgctggaca
    12121 ttttcatcca acagaagtga tttccaataa attatcttca ttagctttat tcagcaaagg
    12181 aatcatgttg catgtttccc gtccagtgcg ttgggattca gaccatgtgg ttttgatgga
    12241 tgatgaattg caggaaatcg ctaaagaatt agtccgtaac gaattgttgg aaaaaacaaa
    12301 tatcggtctg gatttctttg atgcaacgat caaccgtatc gctgcttggg tcatcggtac
    12361 gagaaataca caaaaagcac tgctgaaagc tatgctggaa ccagttgagc gattgaaaga
    12421 aatggagctg gcttttgact ttacttcaag aatggcttat acggaagaat tgaaagattt
    12481 cccgtatgcc gatgtatgga attatttctg ttacaaaaat caagttccag ttggtttgga
    12541 ttggctggaa gaagtccagg aatatcaaaa agacgtgttg gaattaagaa aataggagtg
    12601 tggctatatt gaaaaaaata gatattttac aagcgcctta tgtgaaagaa atgatcgaaa
    12661 cgacgtcgaa tctttatcgc ttgggatggg atgaaagaaa tggcgggaat atttcttatt
    12721 tactaaaaga agaagagatc acaccgttct tggatcctga aaaagtgatc cgccgcatct
    12781 cgatgatttt tgatgccagc cgtttagcag gatgctattt tattgtaact ggttcaggaa
    12841 gatattttaa aaatgtagcg agcgaacctg ccgagaatct tggaatcgtt cgagttgctg
    12901 aagatggcaa aacactggaa cttttgtggg ggctggaaaa cgaatgcctt cctacaagcg
    12961 agttgccaag tcatttcatg agtcatatcg cccgtttaga agtagaccca gataatcgga
    13021 tcgttatgca ttgccatgct agtcatctat tagcgatgag ttttacgcat gaattagacg
    13081 aacgttcctt tagtcgtacg ctatggcaaa tgtgtacgga atgcttagtt gtttttccag
    13141 aaggaatttc catcattccg tggatggttc cgggaacaaa tgagattggg gaagcaacag
    13201 ctgaaaaaat gaaagaaact cgattggttt tatggccaca acatggaatc tacgggtcag
    13261 gaaaagacat ggatgaagtc tttggtttga tcgagacagc agaaaaggca gcagaagtct
    13321 atacgtacgt caaagcgcaa ggaccaatct tgcaaacaat cacggacgaa aacctgtggc
    13381 atttagcaga tgcgtttggt gtcactccaa aagcaggtta tttggaggaa gttcatacga
    13441 aagcaggtgt ctgacatggt gaaaaaagct ttttgcatga aagtttaccc agaaaaacaa
    13501 gaagaataca aaaaaagaca tgatgaactt tggccagaaa tgcgtacagc acttcgtgaa
    13561 cacggggtca tcgactattc gatctatctt gatccgagta caagtttgtt atttggttac
    13621 ttagtgatcg aagatgaaaa gttatgggaa aaaatggcaa atacatctat caatcaaaaa
    13681 tggtggaagt acatggaaga tatcatggag acaaatccag atgcctctcc agtcagttgg
    13741 gaattgatgc cagtctttga actgtagcaa caagcaactg ggcaaccggt tgcttggggt
    13801 tctttcgttt ttattgtgaa aaaaaataca aattaggagg aatagaaaat gagtaatcga
    13861 atgattttaa atgaaacatc gtattttgga aaaggagcga ttgagtcgat cgtccctgaa
    13921 tttcagcagc gaggattcgg caaagcagct gtcattactg acaaaggatt gatcgagcac
    13981 ggtatagcaa caaaagtaac agaacttcta gataaagcag caatcgctta tgctttatac
    14041 gacgggatcg tacctaatcc aacgatccaa aatgtaaaag atggagtggc ttttgttaaa
    14101 gaggcagagg cagactgttt gatcgcaatc ggtggaggtt cgccaatcga tacggcaaaa
    14161 gccattggga ttattttgac gaatccagag ttttcagacg tcattagttt agaaggtgtt
    14221 gctgatacga aaaatccatg tttacctata ttagctatac caacgacttc tggaacagca
    14281 gcagaagtaa cgatcaacta cgtcatcaca gatgaagtaa atcaccgaaa atttgtttgt
    14341 gttgatcctc atgatatccc aatcgtcgcg ttcatcgaca gcgatatgat gatgggaatg
    14401 ccgaaaaaat tggctgcatc aacaggtatg gatgctatga cgcatgctat tgaagggttt
    14461 atcacaaaag gcgcttggga aatgactgat atgctgcatt taaaagcaat cgaaatcatc
    14521 ggccattctt tggaagtttc tgtagatggc gatcaaaatg ggcgagaaaa aatggcatta
    14581 ggacagtata ttgcgggaat gggcttctca aatgtcggtt taggactggt acacgggatg
    14641 gctcacccgt taagtgcatg gtacaacatt cctcatggcg tagcgtgtgc agcacttttg
    14701 ccaactgtca tgaaatataa caaagaatat actggagaaa agtaccgtga gatcgctctt
    14761 gttctaggga ttaaaggagc agctgaaatg tctttagaag atgtaagaga agcggcttgt
    14821 ggtgagatcg atcggttgag caaagcggtt ggaatcccag aaaccatttc tgaattaggt
    14881 gtaaaagaag cagatattcc agcaattgca gaagatgctt tgcgagatgt atgcacacca
    14941 ggaaatccaa gagaaacgac agtagaagaa atcattgctt tatatcaaag tttgatgtaa
    15001 aaacgaagcg gatcttcaac attaaattgc ctgtaaagag aaatggtagt ttatatctgg
    15061 atatctcttt taaattgatg gtatttccgc tataattttt cttataaaga aataggagaa
    15121 tattatatgg aaaataaaaa gccttatgga actgtattga tcaaagcctc taaaatacta
    15181 gactatttag cggaaaacca agatgtctct ttgcagacga tcgcaaaggg tgtgggaatg
    15241 acctcttcta cagtcttgaa aatactagat actttgttga tgatcggcta cgtaaataaa
    15301 aattcagaga aaaactatcg tttaggtgca aagcttattc gctacgcgaa taaaaacatt
    15361 gaacaaatcg atttggtaga gttgacttta ccgtttttag aacagctgca gcaaaagatc
    15421 gatgagacta tccacttagg ggtattggac aacaatgaga tcttgtacgt gaacaaacta
    15481 gatccaaaac atcaaacgat ccgcatgtct tcaaaaatcg gaatcacccg accattatac
    15541 aattctgcga tgggcaaagc cgtattagca gaattcagtg aagagcagta cactgattat
    15601 cttgaaaagc atccgttgat cccttacaca gaatatacga tcaccaatgc attacggttg
    15661 aaaaaggaaa tccaaacggt aaaagaaacc cacatcgcat ttgacgatga agaaatcgaa
    15721 cgtgatattt tttgtatagg agcctcaatc gtgaaaaacg aacaaatcat tggtgcattt
    15781 agtgtaagta tgccgaaata tcgtttaaca gatgagacaa aagaacaaat aatcagtgcc
    15841 ctcaagcaaa caaaaagcga aatcgagcaa agaattcaaa aataatgaaa aaagcgtttg
    15901 caatttttct tttctgccgt ataatatggg tatagatttc caagttataa aataaaattt
    15961 ctaaatagga aataggtgga gaagatgaaa aaaattgata ttttatctcg attgaaaaac
    16021 gccggagtga ttgcggtggt acgcggaaaa agcaaagaag aagctttgaa tgcttgccac
    16081 gcaatcatca aaggtggtct aactggtatt gagttgactt ttacagtccc tcaagcagat
    16141 caagtaatca aagaactttt gtcattctac aaagaccaac cagaaatagt gattggcgca
    16201 ggcacagtat tagacgctgt cacagctcgc ttggctatct tagctggcgc agagtatatt
    16261 gttagccctt cctttgatca agaaacggca gagatgtgca atctgtatca aattccttac
    16321 ttaccaggat gtatgactat taccgagatc aaaacagcgc tgaaaagcgg ggtagatatc
    16381 gtcaaactat ttcctggaag cgcttatgga ccaagtatca tttctgcctt caaggcgccg
    16441 atgcctcaag tcaatatcat gccaactggc ggtgtgagtc tggacaatat gaaagagtgg
    16501 ttcgatgctg gtgtcgtaac agtcggagta ggtgggaacc ttttagcacc cgcagctaca
    16561 ggagattttg acaaagtaac ggaagtcgcg caacagtatg cagcaaaaat gaaagaaatc
    16621 aagaggtagc tgcttatggg aaaagtagtg acactcggag aaatcatgtt gcgattgtcc
    16681 acagagcagg gaacccgtat cgattcaagt gaacgattcg ctgcccatta tggcggaggg
    16741 gaagcaaacg tagcaatttc tttagctaat tacggacatg aagcgatttt tgctacaaaa
    16801 gtacctgata acgcattggg aaaagcagtc aaaaagcact tgcaatgtta tggagtagat
    16861 acgaaacagg tattatctgg tggatcaaga ctaggtactt attatatgga aactggtagt
    16921 ggcgaacgcg ccgcctcagt gatctatgat cgtgccggat caagttttgc tgaaatggat
    16981 catttggaat ggtcgctaga ggagttattc caaggaatag atatatttca tgtttcagga
    17041 atcaccccag ctttgtctaa aaaatggcaa aaaatgacga aacaggtgat agaagctgca
    17101 aaaaaagctg gctgcctgat cagcttcgat atcaactatc gtggaaaatt atggacacag
    17161 caagaagcca gcgaagtaat ccatcagctt ttacctttag tagatatctg ctcagctgga
    17221 aaaatggatg ctctttattt gttaggtatc gagaaagcgc cagaagcaga agaacaacct
    17281 ctaatttatt attatcaaaa aatgcaggaa aagttcccga atattcaggt gttttattcg
    17341 accaagcgta ccgttcattc agcaagtgaa aatgaattga aggggacatt gtggatgaac
    17401 catgaatatg tggaatccca agtccataga atcaccccaa tcgtggatcg agtaggtggc
    17461 ggagatgctt ttgcaggtgg cgtgctccat gggattttat ccaatatgtc tccgcaagaa
    17521 atcattgatt ttgctacggc agcttctgcc ttgaagcata cgatccatgg ggactgcaac
    17581 caatttagtc aagaagaagt cgcacaattt ttagcatgcg gctctggaaa aatcattcga
    17641 tagaaggagt agaagaaaat gcaagaaatg gaaacacgtt atacacacag tccagaagat
    17701 atccgtcatt attcaacgga acaattgaga aaagaatttt tagtagaaaa ggtatttgaa
    17761 ccagggaaaa tcagtttgac ttatacgcac aacgatcgaa tgatctttgg tggagtgaca
    17821 cctactgaaa aagaattaga aatcacatta gacaaagaac taggtgttga ttacttctta
    17881 gagcgtcgtg aactaggtgt gatcaatatc ggtggaccag gattcatcga gatcgatggg
    17941 caaaaagaag aaatgaaaaa acaagacgga tactatatcg gaaaagaaac acgacatgtt
    18001 gtattttctt ctcaagatgc tgaaaaccca gctaaattct atattagttc tgttccagct
    18061 catcataaat atccaaatgt aaaaatcagt atcgaccaag taaaaccaat ggagactggt
    18121 gaaggcctga cactgaatga acgtaaaatc taccaataca tccatccaaa tgtatgcgaa
    18181 agctgtcaat tacaaatggg ttatacgatt ttagaaccag gaagcgcttg gaatacgatg
    18241 ccttgtcata cacatgaacg tcgtatggaa gcttatgtct actttgatta tgccaatgaa
    18301 gatacacgtg tgttccacat gatgggcaaa ccagatgaaa caaaacattt agttgtagac
    18361 aatgaacaag ccattatttc gccaagctgg tcgatccatt caggtgtagg tacaagcaat
    18421 tactcattca tctgggcaat gtgcggagaa aatatcacct atacagacat ggatatggtg
    18481 caaatggatc aattaaaata agcataaggg agagaaaatg atggaattta gtatggatat
    18541 gtttcgttta gatggaaaag tagcattagt caccggtgct gtctatggta ttggatttga
    18601 gattgcacgc tctttagcta cagcaggagc aaaaatcgta tttaataact tgaatcaaga
    18661 atcagttgat gaaggtatag cgcattataa agaagcaggc atcgatgcaa aaggttatgt
    18721 ctgcgatgtg accgatgaag aagcggttca agcgatggtt caacagatca aagaagatgt
    18781 aggttcagtt gatattctag tcaacaatgc agggatcatc aaacggacac cgatgatcga
    18841 aatggatgcg gcagacttcc gccaagtaat cgacgtggat ttaaatgccc cattcatcgt
    18901 ttcaaaagca gttattccgg atatgatcga aaaaggcggc ggtaaaatca tcaatatctg
    18961 ctccatgatg agtgaattag gacgcgaaac ggtcagtgct tatgctgcgg caaaaggcgg
    19021 attaaaaatg ctgacgaaaa acatcgcttc tgaatacggt caatacaata tccaatgtaa
    19081 tggaattgga ccaggttata ttgctacacc gcaaactgct ccattacgcg aaacacaaga
    19141 aaacggtgaa cgtcatccat tcgatcaatt tatcgttgga cgtacgccag cagcacgctg
    19201 gggcgagcca atcgatttgg ctggaccatc tgtcttcttg gcttctagcg catctgattt
    19261 cgttaacggc catgtcttgt atgtggacgg aggcatactt gcctatatcg gaaaacaacc
    19321 ttaaaagagc tttagtacac tgtttagtgg agaggttgtg aaatcagcgg attgaacgaa
    19381 taccgaagga cagctgatta aacatcattt attcgaaaaa agaggctggg acagaagcat
    19441 ttaactccaa gaaataagaa ggaattcacg aaaattgctt ttcaaatttt tgtgaatttc
    19501 agcttatttc cgaaggagtt gcttttactt ccgccgtttg tacgtgttta gagcgtgaga
    19561 caaaagtgtt ctttactttt gtcccacgct cttttctgtg cgtcttccat acttcactaa
    19621 gtcgatccct cttgcttcgc accaatcctt cactggtccg gaaaagatca gagattgttg
    19681 gtgtagggcc gcggtattcg ttgcgccgac catagtataa agcagacgaa gctcttcttg
    19741 ccattgcttc atcagtataa tagtttcttc tacgccgtga ttcatcagat gcgtcagtac
    19801 ggttcctgaa gtacccacgg catttgcgcc gagacaaagg gctttaaata tgtcataggc
    19861 attgcggata cctccggagg ctaggatttc catagaagtg tctgcttcat ttgcttcaag
    19921 caatgaagag actgtcgatt gcccccagtc agctaaataa ctcagttccc gcttggagcg
    19981 acgggcgttt tcgatctggg tgaagcttgt tccgcttcga ccgctgatat caactgtatg
    20041 aactcctaaa gcagctagat cattgagcgt ttctcttgtc atgccaaaac cgacttcttt
    20101 tacgatgagg gggacgtcaa tggctgtttg agtttcttga ataagtactt tccaattggt
    20161 aaaatcacgg tctccctcag gcataaccaa ctcttgaggt gcattcaaat gaatctgtaa
    20221 ggcatctgcg tgaaacaatc ggatagcttc tttggctctt tcgacagaag tccctgcacc
    20281 gatgttggca atgattttcc cgtgaggata ttcttgtcgc ataatcgtat aagtatcggc
    20341 taaagaaggg actttcaagg cagcgctgac tgaaccagtg gcaatcatca aatctgcttc
    20401 tcttgcgacg attgcaagat cttggttgat tttttttgtt ttttcgcttc cgccagtcat
    20461 cgcattgata taaaatgggc tgcttagtgt aaacccaact gcctgtgtac ttagatcgac
    20521 atcagaaact gctatttgcg gcaaagggtt atggatgatc cgtacgaagt caaattcgtt
    20581 tttttgttta tcatgaaagg ctttagctag tgaaacatgt tcgtcttttc gattcatcgg
    20641 ttttcctttc tttgatcgct atagacatgt aacggcagtg gagtgatttc tgctttttcc
    20701 catgcactca ttaaaggaag aatgccagat ttctggtcaa caattacgat tccgcaatcg
    20761 cccccacctg caccagaaga ttttgcggct ccttcatact gttcagctaa attacacaat
    20821 ttgttcaaag caggcgtttc gatgacgacc ccagtgattg cagataaatc atgcagtaat
    20881 tgtcggtttt ttcgaatcat ctgttggatc aacgttacat tattttcttt aaaccctttg
    20941 atcatttcgt tgacacattc tgtactattt tttaagaaaa gctgataagc caccatttta
    21001 tcttctctcg aacggtgaac ttgatcgacc aaatcagaag tagaggcagg gctacccgtc
    21061 caaccaatca ataaacgtaa atcttcagga gcaatcaatg gctcaatgga tagacctggc
    21121 caatccaggg caagtaactc actgatagaa tgctgagttt cttgttcttg gagccaagga
    21181 tggtcgaagg ttgagaaagc gatccagcca ccataacagc tagcagcgat gtcgccgcag
    21241 gaaccattat cttgaactgc taaattggct agtgctgcaa tcttgaaaat ctccaactga
    21301 gataaattca acgcgtaaaa aacattcaag gctttgaccg ttgcaacggt cacggcaccg
    21361 ctagaaccca atccatattt ccggccattt gaactgtcta actcacttgt tactttcaga
    21421 tcataaaagg ataaaagaat gtttttttct tgtgcatact tttcagtcaa gcgaatagca
    21481 gcaaggatat aatgaaaagg attttcccga atatctaaaa ctaattctcc gttgcgtctt
    21541 gtccaacgta caggcatccc actatattgt gcagattgga tacttccgac ttttcgtgca
    21601 gattctacag tgactgtcac gaattgatcg actgcagcga taactgctgg atggcctgtt
    21661 tcaacaactg catattctcc ggcaatatag agtttgcctg gtgcagatac ttcaatcata
    21721 aaaatctccc tttattcaat aatcgcaatt cctggaccag catgtgcagt aattacttgt
    21781 tctttgctaa ataatttggt aaatgtttct tgtacctcag ataaatggct gttttccact
    21841 aaaactttga cattagggcc agcatccatc gtaaagtagc atggaatgcc ttgctttctc
    21901 aattgtcgca ccgcatccat cgcttttaag ctgtcaggag accagtaagt gaatggaggc
    21961 tgggcagcta aagtcgttcc atgcattttt agtccgtttc tttccatcgt ttctccgaga
    22021 agttggaaat cttttgtttt gattgcttgt ttcaattgat atagatcccc ttcaacggaa
    22081 tctaaccagc cttgataaaa actggatgtt tcgactgtcc gacgcatccc atttctgctg
    22141 gacacttctt ttttctggtc gttgatcaaa acgaaaacca ttgctaaatc gtcttcgaaa
    22201 gaatcggaag ggactggctt agcgtaagaa cttaagtcgt catggccttt ttcccattcg
    22261 acgaaaccac cgaaaatact tcggcaagct gaaccagacc cgcgtcgagc aaatcttgaa
    22321 agagagagat catctaatcc aagcttaaga gcagtattgc aagcgcctgc taaagcagct
    22381 agcccgctgg cagaggaggc taatccagct gcggtaggaa cgaaattctg actgattact
    22441 gaagctttta gtgagaggcc ggcttcttta cgaaaaaggt caagaaactg actgactttt
    22501 tttgttgctt tttcgtcttg caaggtgcca tctagataaa attcatctac catatagcta
    22561 tcagaaaaga tgacttctgt ctctgtataa aatgcatcca atgttaacga aaggctgttg
    22621 ttcattggaa ggatgagttc ttcatttttc ttaccccaat atttgattag agcaatgttg
    22681 gtatatgcgc gtgctttgcc tttaaacata ggtatgtact cctaatcctt gtatccaagt
    22741 ttcagcagca cccgcatctt caagtgcttg actgatttct tgggcttttg tttttgtttg
    22801 tgctaatgca atcatacagc caccgcgtcc accgccagtt aatttggcac ccaatgcccc
    22861 ggtatctttt gctgtttgga tcaatagatt aagaaaatca ttgctgattg tcagcttttc
    22921 cagaagtgat tgtgattcat ccattgtctg tgccaacgtt tctggtgaat tttcaatgat
    22981 tgcttgtttt gcttgcttcg tcaagtatcc taatttttgg atcatttgtc cagtttcatg
    23041 aggctgggtt tcaaagaggt gagcgacatc tttgacggct tctcttgttt gtcctttgat
    23101 tcctgtatca gcgacaatca aaaaagcatc gatgttcaaa gaaaagtagt cgaaaggatg
    23161 cccttttgta aaataaatcg gctgcaagct gctagtagcg gctgcatcga ttccactagg
    23221 attaccgtgg gcgatttttt ccgaaagctg gacattttct aatagtattt cacgagacaa
    23281 aggaaatgct aagtaatcat aaaaagcacg agtgactgct gtggcgactg cagcgcttga
    23341 tcccattcca cgttcagcag gaatcgtgct ttcaatggtc agttgcaagt ttttttggat
    23401 gtcatgctgt ttttttaagt gaatgaataa ttttttgata ttttttaatg catgaggagc
    23461 ttcggctaaa tttcctgaat agtaggaaga aaccagttga tcgtccattg catccagctc
    23521 ttcaaggaat gcggtgactt ttgttgcata gaaaggaaag gcaatcgctg gttctccata
    23581 aacaaccgcg tgctcgccca tcaatatgat ctttccgctt gattctcctt ggccatagtt
    23641 tgccataatc taactccttc tcacgtaaaa gtgattttta agcaaaaaat aaagcgccca
    23701 aaacgagttg cgctgcttca cgaatttttt tgtccactaa gggtctcaaa aaacattcct
    23761 cctttatttt actaaaacca tctcagaagt aaaactaaga atacgttttc taaagaaaaa
    23821 acactttttt cttaagaatt ttttgacttt ttttcttctt tgactagaaa aaaatacaat
    23881 tgacactact agatattggg ggtatattta aaaaggttgg agttaaacac aatatttagt
    23941 gatgaagtga gctggaggta aatgagcgtg atcgttttag caggtacaat tggtgccgga
    24001 aaatcaagtt taacagaaat gatggctgaa cactttgata gtcaggcatt ttatgaatcg
    24061 attgatgata atgaagtctt gcccttattt tatgcaaacc ctgaacagta tgcttttttg
    24121 ctgcaaattt attttttgaa taaacgtttt gcaagcatca aacaagcaat gaaagatgac
    24181 aataatgtat tggaccgttc gatttatgaa gattcactgc ttttccatct aaatgcagat
    24241 ctaggaagag caacagaaac agaagttcgt gtgtatgatg aactgctgga aaacatgatg
    24301 gaagaacttc cctatgctgc ccataaaaaa catccggatt tattagttca tattcgtgta
    24361 tcttttgata cgatgttgga gcgtatcgaa aaacgtggtc gttcgtatga acaattgtcc
    24421 tttgatccaa gtctatacga ttattacaaa gaattgaaca gacgttacga tcaatggtat
    24481 gaagaataca aagaaagtcc aaaaatccaa atcgatggag accgctataa ttttgtggaa
    24541 gatcctcaag caaaagaaga agtcttgaaa atgatcgaag aaaaattagc agagattcgc
    24601 caaaccaaag tagccatttg atttacagtt gctcaaataa agggtaaacc tttatttgag
    24661 cgactttttt ctttatactg gaaacaaatg tcaggaggat agagtatgcg ggaaaaacac
    24721 tattttaatc aagaccgtaa tttttggatc aatgtacagc aaaacgatgt gctagctgtt
    24781 aaaaacttaa aagaagaata tggcattagt gacgagatgc tgacttattc tctagataaa
    24841 aatgaacggg cgcgcgtaga gtacgattct tttgatgaag cgttactgct tgtatcaaat
    24901 gttccgcacc agcaaaaagt cgataatcat tatgaaacta gtcctatcgc ttttatttta
    24961 aaagaagacg gtttgtttac atttaccacg ccgaataccg agtacgtgat tcgactaatt
    25021 cgatcgcttt tggaacgaat gccagacatg agtgtataca gtttattgtt tcgtacattg
    25081 tttttgattt ctgattcctt ttttccttta atcgaggaag tgaatagtga acgtcagcgg
    25141 cttaatctga aattaagaga aaaaacaacg aacaagaatc tgctgcaact ttcagattta
    25201 gagatcggtt tggtttactt agttacagga acaaaacaaa atgtagtact tttagaacaa
    25261 atcaaggcat tggctattta tcgcaagttg tcagaaaagg aaaaagaaga gttggacgat
    25321 gcgctgatcg aagcacgaca agcagttgaa atgacgaact tagcttcgca gattttagac
    25381 cagctctctg gcacttataa taatttgctg aataacaact tgaatgacac gatgaaattt
    25441 ttgacggtct ggtcgctgat cttgaccgtt ccaactattg tgacaggatt cttcggaatg
    25501 aatctccagt taccctttac ccattccgtt ttcggatggg gaatcgcctt gatcatcagt
    25561 ttggtcttat ctatttggat gcttattgca ctgtggcggc ggattcgttg atctagtctt
    25621 tgatctgcaa accaagggct tgggcgatca caagtgcttg ctcttggttg acgattagtc
    25681 ccgccaaaga atcttgagat aaagcgattg ctgtgaagaa attagtcgta aagtctaatc
    25741 ccttcagaga agttcgaaac cagttcgaac cggtcaactg attgtcagac aaaaaaaggt
    25801 ttttccaagt gacctcataa aattcgctgt cttctaattg acaatgatcg aaggagacag
    25861 ctttcaagtt tgtactacta aaggaagcca tcgaagcaat acaatcagtg aaagtacagt
    25921 ctcgaagata actttcggca aaattcgtac ctgttaattt acattgatga aacacgactt
    25981 ggtggaaact gccaccaatc cactccagat tggaaaaatc acatttttca aaaatgacat
    26041 tactgcattc aaaatgacgt aaaactgttt tttgcatcgt aagtttttgg atatgactct
    26101 ctcttaatac catatttttg gcatcgagat agctttggtc acccccgttg atcagaagac
    26161 cagagaagat ctcctcatct tctaaataaa actctccctt tgtaagaaga ggaagagaag
    26221 gtggaatagg ttcgattttt ttcatgtggg ttcacctcgt ttttctttag tatagcaaag
    26281 aagcgggaat cactgctaga aagtctgcta atctaatcat tttcagaaaa ggaagacaaa
    26341 ctgtatgaaa aacattgaca aaaagattag tttttgctat actatgatgg ttgagaaata
    26401 aaagcagaag cacccgcttc tcgccttagt tgataatgtc actgggctag atagttttca
    26461 ctggttctat taggtgaagt acgcgcgggt tctgtaagag aactcgtttt tttattgccg
    26521 tttattcggt aagcttttct ttctctcata atatttggag gtgaatgacc atagcaaagg
    26581 atatgatggt taacgacggc attcgtgcac gagagttacg attgatcggt tcagatggtg
    26641 agcaattagg agttaagaca aaagcagaag cattgaatat tgcagaacaa gcaaacttgg
    26701 atcttgtgtt agttgcccct ggtgcgaaac caccagttgc gcgaatcatg gactacggta
    26761 aataccgttt cgaacaacaa aagaaagacc gcgaagcgcg taaaaaacaa aaagtgatca
    26821 atgtaaaaga agttcgctta agtcctacga tcgacttgaa tgacttcaat acaaaacttc
    26881 gtaatgcacg taagttcttg gaaaagggcg ataaagtgaa agcttctatc cgtttcaaag
    26941 gccgtgccat tacccataaa gaaattggtc aaaaagtctt gaatcgctta gctgaagaaa
    27001 ctgcggatat tgcaacagtg gaacaaaaag cgaaaatgga cggacgcagc atgttcttga
    27061 cgctggcacc gaaaaacgac agtaagtaat ttgaatgact aactggctgt tggacagccg
    27121 atagtcggat aacgaaagat tttgaggagg aaatatagtc atgccaaaac aaaaaacaca
    27181 ccgcggatca gcaaaacgtt tcaaacgtac tggtaaaggc ggattgaaac gcttccgcgc
    27241 gtttacaagt caccgtttcc acggcaaaac aaaaaaacaa cgccgtcaat tgcgtaaagc
    27301 aagcatggtt tctagtggcg acttcaaacg tattcgccaa caactagcaa aaatgaaata
    27361 agaagtacaa ataacagaat ctgaagattt atttttatta ggaggaatta tacatggcac
    27421 gtgttaaagg tggaacagta actcgtaaac gtcgtaaaag agtgctaaaa ttagcaaaag
    27481 gttactacgg ttcaaaacac actttattta aaacagcaaa agaacaagta atgaattcat
    27541 acaactacgc atatcgcgat cgtcgtcaaa agaaacgcga cttccgtaaa ttgtggattg
    27601 ctcgtatcaa cgcagcagct cgtatgaatg gcttgagcta ctcaaaatta atgcacggtt
    27661 tgaaagttgc tgaaatcgac atcaaccgta aaatgttagc tgacttggct gttaacgatg
    27721 cagcagcatt tactgcattg gctgaccaag caaaagaagc tttggctaaa taagctaaac
    27781 aattataaag aggaaagaag ataacgaaaa gttatgttct ttcctctttt tttatttaca
    27841 atcctccatc aaatgaaaca ggacaataac gtataagtct aaaatagcat aattaaggtt
    27901 ttttaattga aaactgaaca tttatggtca ccctttaatg aaagcgctaa ctacataagt
    27961 tagaatgagt ttgactgata tattagtttg cttatcagaa aaacaaaatg gacggaggaa
    28021 taacatgagt gatattatta ttggtatttt gttagttatc tcattctttt tcatggtctg
    28081 gtactgtgta aaaggctaca acttgatggt cggctttgcc atcatggcta ctgtatggat
    28141 gggattagcg ctagtcggaa acacgttttc accaaacccg gcgatggaag ggcaaggagt
    28201 aattgatgtg ttgacgcata tctatacgac ggggccagct gagtacgcca aatcgatctt
    28261 ggtaaatgtc ttctttggtg cctttttcgg gcgtgttcta gtagatagcg gaatcgcagc
    28321 taccctgatt cgaaaggtag ttgaacttgg aggggacaag ccaagaatca cgatgagctt
    28381 actatgtatt gtaaccgctg ttatattcat gtcgatgaca ggaatcggac cagtgatttc
    28441 tattgcagtt attgtgttgc caatcttgat gtcattaggt atttcagtac cggttgcact
    28501 gttctcattt atgggctcga ttatggctgg aatctttgct aatatcgtca atttcaaaca
    28561 atatcaaacg atttatgctg gtttcaatcc agcggctgaa agctatactt ataatgacta
    28621 tttccaaatt ggtatgattg ggatgattgt aagcttagtt gtggtactaa cggtcgctaa
    28681 tatatcaatg aacaaaaaga aacgctatgc aatggcagca aacgtaccag ctgaaggtgg
    28741 cgacgcacca atgatctcat ggttagccgt attattacca gttttagggg tcgttctttt
    28801 agatcttcca atcatcttag gttttattct agcaggtatc tgggcattgc tgtttactgg
    28861 aaaattgcgc ggaggttata aagaaatctg tcgtcagttt gcgaaattat ttacagatgg
    28921 cgcagtagat gttgcaccaa tggttggttt cttgatgacc ttagcgatgt tcaataattc
    28981 agctgcgtat gcttctcctt atttttcagc tatttttgga gattggatac cgcaatcacc
    29041 attagtcttg gcaatcgtat ttgctatatt gacaccacta ggatttttcc gcggtccaat
    29101 gaatctagtc ggttcaggct cagctatttt agctgtcgta ttagctgtta atccaacgat
    29161 gtcgccagcc tttttgttcc cgttatttgc aatcacaacg attgctcctc agcatttaga
    29221 tattacacaa tcttgggttg cttggggatt aggttataca aaagtaactt ctcgagaata
    29281 tatgaagaaa tccatcccaa ctggttggat catcggagcg atcttatgcc tgatcacgtt
    29341 cttattgtac gggaacgcct agtaaacaaa acgtcgataa aaacgattag atgaatggag
    29401 gaaaagaaaa tgaaacgttt tgtgcgaatg ggaattgatg ttggcggtac acatacgaaa
    29461 gcagtcgcaa tcgataatgc gacgcacgaa atcatcggaa aatcttcagt gaaaacaacc
    29521 cacgatgatg tccgtggagt agccgcaggt gtcgttcagt cttttcaaaa ttgtttgcgg
    29581 gaaaataata tcagtccaga agatgttgtg tttgttgcac attctaccac acaagcgact
    29641 aatgcactga ttgaaggaga cgtagcaaaa gtcggcgtaa tcgggatggc aaaaggtgga
    29701 ttagaaggtt ttttagcaaa acgacaaact agattgaacg atatcgattt agggaataag
    29761 aaaaaaatag agattgtcaa tgcattctta cctgtcaaac acttaaatgt agaccgtgtt
    29821 tcagaaacga tttcttcttt agaaagagaa agagcggagg tcttggtttc ttctatggca
    29881 tttggagtag ataatgggga accagaacga gttgtttatg aagcagcatc ggtaaaatcg
    29941 atccctacaa cgatggcttc tgacatcaca aaattatatg gattgacaag aagaactcgg
    30001 acagcagcaa tcaatgcatc tattttacca aaaatgctag atacggcaac ctctacagaa
    30061 gattccgtac gagaagcagg ggtcaatgtt tctttgatga ttatgcgcgg agacggcgga
    30121 gtaatggaaa tcaatgaaat gaaaaaacga cctgttttaa ccatgctttc cggtccagca
    30181 gcatcagtca tgggctcttt gatgtacttg agagcgtcta atggtgttta ttttgaagta
    30241 ggcggaacaa caaccaatat cggggtcatt aaaaatggac gtccagcaat cgattattca
    30301 attgtaggtg gccacccaac atatatctct tcattagatg ttcgggttct aggtgtggca
    30361 ggcggttcga tggtacgggc taatcagtca ggaatcatcg atgtcggtcc acgttctgca
    30421 catattgccg gtctagatta tgcagtattt acggagacag aaaaaatcaa aggtcctaag
    30481 gttgaatttt tctctcctaa agaaggagac cctgctgatt acgtcaaggt agtgatggaa
    30541 gacggagaag aagtgactat tacaaataca tgtgcagcca atgtccttgg attagtgcaa
    30601 gaagaacatt tttcttatgg aaatgtccca tctgcacgaa aagccatcca agcattagct
    30661 gattattgtc atacgacggt tgaagacatc gcagaacaga tcatggaaaa atcctatgct
    30721 aagattgaac cggtgatttt agaattggca gacaagtatc acttggagaa agatcagatt
    30781 tctcttgtag gtgtaggggg cggcgctgcc tcattgatta cttactttag taataaaatg
    30841 ggtgtgaaat attcgattcc tgaaaacgcc gaagtgattt cttctattgg tgtagcactt
    30901 gcaatggtcc gagatgttgt agaacgtatc atcccttcgc caagtaaaga agatatacga
    30961 tcgttgaaaa atgaagcgat gaataaagcg attgaatcag gcgctacacc agaatcgatc
    31021 gaagtccacg tagaaatcga tccacagact tcaaaagtaa cggctatcgc tactggttct
    31081 acggaagtga aagcaaccga tctaaccaag gagatcacaa cagaagaagc actagagctt
    31141 gctgcagagg atatgcgatt aaataaaaat gaagtttgct tattggaaaa tacacctttc
    31201 ttttatgtat gcggcgaaca gaatcgttca aaaaatgctg gaagtcttcg gatcatcgat
    31261 caaaaaggat ttatcaaggt ccaacgtgga catgcttcat gtatgaaaac aactgcagcc
    31321 aattatatga cagctgtcga acaactttgg gaagatatgg cagtttatca aactgaattg
    31381 attgcccgac cagaatttta tctctgctta ggagcaagag tctctgattt tacagcgacg
    31441 gatctggaac aactacagct tttgatggat ctggaagtat ctactatgga accggaggaa
    31501 gaagtgatcg tcgttgccgg aaatatcaaa caaacataag ttagaagagt tactaaacga
    31561 attacaacag atccccgaag aaatttgggg attctaccag tttcggcgag atttattttg
    31621 gaaaaagatt cctttgtcca agcagaaaat tcttatacaa caatcaatcg attgtggtat
    31681 cgaaacggca tgtagcatca aaaaaaagta cccttttgcc gatgtaggag aaatttgtga
    31741 acaaatggct gttccaatcg tttcctgtga atcagagcaa ataaacgaac gaatcacttt
    31801 tgcaacttat gcagaagatg aagggattcg attgatgacg gagccgttag aaaaattaaa
    31861 atgttcagga ctagcttcga tctctaaaga aacagcgcaa gctttgatta ttggacatga
    31921 actatttcac cacatcgaag cttctgttaa gggaatttat acgcaaagcg aaaaaattgt
    31981 gctgtggcgg ttgccttttt atacacatca atcaaacatt cgtgcattga gcgagatagc
    32041 agcgatgagt ttttcaaaag agatgaatca aagcgatttt tcgccttatg tattagaggc
    32101 agtcttactt tggccatata atgaaattcg tagccaaggg atcttagaag aaataaaaaa
    32161 aatagaaaaa agatgtacgg agtatgattt agattacaaa taaagagact gtgacaaaag
    32221 tcgtgtcaca gtcctttttt tccgaataaa cgatgttctc ctgtcaactc ctcggtcaca
    32281 agtcaaaata ttcaacaaac atgagaagct gttttttgaa attgttcctt gtgactcgga
    32341 gatgaacggc aggctcacga cctcttggaa aacggtgttc aaaagcttgc atctgcggta
    32401 ataagtccag ccaagttaaa aacataagaa gctattttta cttggctgtt cttattactt
    32461 gatgcagggc agctttttca caacctctca ataaacggtg tgcaatcagc tgaccctcgg
    32521 tattagctca aaaaaaacgg caaaatatga ggaactattt tgccgtttct ttctctaata
    32581 ctcaggtcaa aacgctgatt ccaaaacctc ttatttgatg ggtctgtcct gaatatcttc
    32641 aaaataatcg gggtaattat gaatcacgta ttcttgctcc tcgataatca tatgtaaatg
    32701 catcgtgcaa agaaatccga cttcatcaaa gttatgatca atcattgatt gaagcattgt
    32761 ctgatgttca tctaatactc gaccccagtc taattctgaa atcgtcaaac gtaaccatct
    32821 gaaacggttc aagtgcgtac tataactttc tagccaatcc caaatttctc gttttccagc
    32881 aatttcataa caagttcggt gaaaatcagt atccaaatca aaaaaagctt ttttattttg
    32941 agcgattact gcctgatcgg tttttccaac ttttcccgcg tataacgggc taatgaagca
    33001 gatcggatat cgatttttga aacataagtg ccggactgtg gaatggtttt tacaagctct
    33061 tgattgcgca gttggatcag tgcttctcga atcggtgtac gcccgatagc tagttgttct
    33121 tctaacactt tatcggagat tttacttcca ggtgctaact cggaataaat gatttgttta
    33181 cgtatagatt cataagcttg ctgttgcaaa ttattgataa ccatttaacc ttcctttcta
    33241 tgcagatata atctattata acataaatcg cttacaaatc gtgtgggcat aaaaacacag
    33301 tctgcaaaaa aagccctgat aggcatgcct attagagcct cgaaaagaaa aatattgaat
    33361 caatctggtc gtacctcttg tatcggtttg acacgaccgc cgattctttt agcaaaagct
    33421 tcggattctt ttttgtcttt gaaaatgagc agattgtcta agtttttttc ccgagtgcga
    33481 cagccagttt tatcgatgta accgtgcatt actttaacga catacatggg gatcacctgc
    33541 ttttttcttt tcattatacg aactactcga cacaatacaa gaaaaatgct tagacggtct
    33601 ttattaagaa tttacatcat ccaaagaagc gtacgcttgg aataactgat ctaactccgg
    33661 attgactttg ctcagcttca ctaatcgttg cgaatcagaa agtaaaaaac agtgtttact
    33721 actgccagta gtttttaatt ttttgtcttc agttcccaca atttcgtagc taaaatccaa
    33781 ccgtgtcccc gtatactttt ggatagttgg atggatgctc actttttctc cgtaatggat
    33841 catttcttta tagctgcaat ttacttccag aacggggatg atgatcccag attcttcgat
    33901 ttttttatag ggaaacccta gatgtaccaa taaggctacc cgagcctctt caaaccagcg
    33961 gatataattg ctgtgatgaa taatccccat ttgatctgtt tcataataaa aaggttctcg
    34021 cacataacct gtaaatttct ccatgactca tgcctcgttt tccattgatt ttttacaaaa
    34081 ataattcatc catttcggaa gtgcttcgct gatttgtgtc ggcaaaacaa tatattgttc
    34141 ttttgccagc tggtctccaa tgaaactatg aagataaata gctgctgcaa tcgtttggtc
    34201 attttgagga aactgggcta aaaatcctgt gatcatccca gctaaagtgt cacccatgcc
    34261 gcccgttgcc atagcagcag aacctaaagg attggtgtag aagtgctggt gaccataaat
    34321 ttttgtctca tgacttttca aaacgagcac agcaccgatt tcctgttgct tctttttgtt
    34381 ttctttatcc cgttgttctt gtatcggtag agaactcaac cgttgccact ccatttgatg
    34441 aggtgtaagt acagtttgtt cgggataggg tagcaaaaga tgattagcag caagtaaact
    34501 taaagcagag ccatcaatca ccagccattg ttcttttgat tggttttcta gtacttgttt
    34561 gaaacgttcc aaactcttag aagaagtacc caatccggga ccaatcaaga gtacatctgc
    34621 ttgctttatt acggaaagaa actggtcagt atccgtccaa actacaatca ttgcttctgg
    34681 cagatgactg tgaagagcac tccgattttt ttcatcagtt actacagtcg tgagacccgc
    34741 tccgctgtgt acggcagcta aagcactcat aatgattgct ccgccatatt gttcattgcc
    34801 gccaatcaaa acgatacgtc caaatgttcc tttatgtgaa atttttggac gaggtgtgat
    34861 gactcttttc actagttgtt cgtttatcag catctaaaaa cctcctttta ctcattatag
    34921 cgtatatttt ttttagagaa aaacagataa gaaaaggaat tgtcagattt tttgaactat
    34981 ttttttctgt aatttgtggt atcatgaaca cgaagactta aataagtgga ggattaactt
    35041 tatgacaatt gattggcaaa aagaagtaga agcacgtaaa gaagacttgc ttgaagactt
    35101 gaaaaatctt ttacgtgtca acagcgagcg cgatgactca aaagtaacac cagatgcacc
    35161 atttggacct ggacctcgcg acgcattgaa acatatgtta gcttatggcg aacgtgatgg
    35221 atttaaagta aaaaatgtcg ataactatgc agggcatatt gatcttggcg aaggagacga
    35281 aacgctaggg atatttggac acatggatgt cgttcctgca ggagatggct gggacacaga
    35341 tccttatgaa ccagtaatca aggatggaaa gatctttgca cgcggatcaa gcgatgacaa
    35401 aggtccaagt atggcagctt actatgcgat gaagatcatt aaagaacttg acttgaaact
    35461 atcaaaaaaa gttcgcttcg tagtcggcag cgatgaagaa agcggctggg gcgatatggc
    35521 ttattatttc gaacatgaag aagaaccaga ttttggtttc tcaccagatg ctgaattccc
    35581 aatcatcaat ggtgaaaaag gaaatgtttc tttagcttta cgcttcaaag gcgacaatgc
    35641 aggagattat gtcttgaaat cttttgtttc cggacttcgc gaaaatatgg tgccaggaac
    35701 agcaactgca gcacttcaag taccaagtgc agatgcagct atcgcgatgg aagaagcatt
    35761 ttaccaattc atcgaagcaa atccagtaag cggaacgatt gaagcggaca atacgtatgt
    35821 aaaaatcgaa ttagttggta aaggcgcaca tggtgcaagc ccacaatctg gtatcaatgc
    35881 aggttctttc ttggcattgt tcttagataa ctatgaattc ctaggatctg ctaaacaatt
    35941 tatccatgta gcagctgctt atgtccatga agatttttat ggagaaaaac taggagtcgc
    36001 ttatgaagat gaaaaaatgg gcaaattgac aatgaatgct ggactatttg cttttgaaga
    36061 aaatggtaca gaggaagcga acttcatcaa tatgaatttc cgttttccaa aaggtgtaac
    36121 agtagacgga ttgcaaagtg acatcgaaca aacagtgggg caagaaggcg caacagttac
    36181 tcgaggtgct cgcgtaatgg aaccacatta cgtaccaatg gatgatccat tggtggctac
    36241 attattgcaa gtctatgaag accacactgg agaaaaaggc tacgaacaaa tcatcggtgg
    36301 cggaacttac ggacgtttgt tgaaacgtgg cgttgcttat ggagcgatgt tcccaggata
    36361 tactgataca atgcatcaag caaacgaatt tatgagtctt gatgacttgt tcagagcaac
    36421 agcaatctat gcagatgcca tctatcgttt ggcgaaataa ttaattaaaa agaggatcaa
    36481 gaattgcaaa ttatttttgc agtcttgatc ctttttttac tttctactgt ggtattcatg
    36541 ataaacatga tatagattca attcatattc tttcttagcg ttcgtgacga ctgtatccaa
    36601 aatcaaccct gttactaatg aaagaagtgc taaagtcatc aatccagtac ttaaaatcgc
    36661 agaagggatg cgtgtaataa agcttgtcag catatattcg cgaatcacag gaatccccgt
    36721 gaccaatcca aataaaaaga agaagatggt ccaaatccca aagaaaagca aaggcttata
    36781 atctttaaac attttaataa tcatcatgat cactttgaat ccgtcagaga atgtattcaa
    36841 ttttgattcg ctgccttcag gacgatcacg ataatcgatt gggatttcaa cgaacttgaa
    36901 ttttttatct aaagcatgga tcgtcaattc cgtttcgatt tgaaaaccag aactcataat
    36961 aggaaaactt ttgacaaaga tacgattgaa tcctcgatag ccggtcatga catcaagaat
    37021 ttttgtttta tacattctag taattgtatt tctaacaagg ttatttccaa aatcgtggaa
    37081 aggtctctta ttctcatcaa agtaggttcc atttgatagg cgatcaccaa tgaccatatc
    37141 tgcttcacca gaacgtaatt tctcgagcaa accatgtaca gcttctgcag gataagtatc
    37201 atcgccatct accattaaat aatagtcggc atcgatatca aagaacattt ggcggatcac
    37261 atttcctttt ccttgacgag gttctttttt tacgattgct ccaccttgaa cggccaattc
    37321 gtatgttcga tcggtcgaat tattatcata gacataaata tctgcttctg gtaattctcg
    37381 tttaaagtct gcaatgactg ttgaaattgt tgcttcctca ttgtaacaag gaatcagaac
    37441 agcaattttt tcttgttgtt ccatttttca ctctccttaa ctatagaaaa caaaagctaa
    37501 attaaaaata cctcataatc aaaggaaatg ctagtttttc ccgctaaata tcatttattt
    37561 aaaaagaaaa tactggaata tatgtttgga aagctctatc taaaaaacca cccactacaa
    37621 aagaaggaca tgatacctct cttttgaata gtgggtgcgt attcgttctc tcactttttt
    37681 gacgtattaa tctgttgcac ctttttttct tgaaatttct aatacggttc ctgtatgagc
    37741 atctgctaaa aattcataat aaacagtttc gccatcttct attcgagaaa gacctccccg
    37801 ataagcactc gaccgaacgg cgaatttcct aataggttcc ttttcaaaag agatccaaga
    37861 accttcgatt gcaccttctt ttagaaaaga tgacttgata ttttcaagta cttggtctgc
    37921 tgacaatact tttttgcgat gataccaaat cgttgaagct accccgctca ataatcctag
    37981 accgattcca actgctaacc cgcctttaaa atagcttagt tcgctttctt catacatgtg
    38041 gacatacctc cttcttctca cttgttactc cttgtgctta ttttaccaca ctctcatatt
    38101 ttctttgaaa taaataaccg ctaccagaaa aaataagcta taataaatac gatcggtctg
    38161 cagaaaaaat catattttat ccgcagatca atataagtct gagaggagtt tagtctgaaa
    38221 tttagacgaa tagcatatgg aagaaaaaac atttcaacga atcaaagagt taacagaatt
    38281 acaaggaaca agcggttttg aacaagatgt tcgcgcttat atggaaagtc atatggaacc
    38341 gctggtagat gagttacagt tagatggttt gggcggcatt tttggtctgc gccatcatga
    38401 agaagcggat gcaccccgtg taatggtcgc agcacatatg gatgaagtcg gattcatgct
    38461 gacacaaatc caagaaaacg ggctttttaa agtcgtacct ctaggcggct ggaatcctta
    38521 cgttgtttct gctcagcgtt ttacacttaa aacctctact ggtaagaatt atccatgtat
    38581 ttcttcttcc gttcccccac atttattgcg aggaacaagc ggacaaaaat cagtagaagt
    38641 aaccgatatt ttgttcgatg ctgggtttga atcaagagaa gaggcaatga gttacggtgt
    38701 ccttccagga gacacaatcg ttccttacgc tgaaacgatc aaaacagcca acggaaagaa
    38761 tattatcagt aaatcatggg acaaccgcta tggctgtaca atggtattag aagctttaga
    38821 agcgttgcaa aatgaaacat taggtcatac attgattgca ggcgcgaatg tccaggaaga
    38881 agtaggcttg cgtggttcta aagcttctgt caataaattc aagccagatc tgttctttgc
    38941 agtagactgc tctgcagcag atgatacagt gacgaaaaat ggcacattcg gacatttagg
    39001 agaaggtaca ttgatgcgta tccaagatcc aggtttgatc atgcttcctc gtttgcgcga
    39061 gtacttgctg gatatcgctg aaacaaacaa tatcccttat caatactttg tttcaaaagg
    39121 cggtacagat gcaggtgctg ctcatacaca aaacgaagga atcccaagta cagtgattgg
    39181 cgtagtggga cgttatatcc atacgcatca aacgatgttc agtattcgcg attttgaagc
    39241 tgcacgagaa atgttgattc aaacactgaa aggtttggat aaatcaacag tgaatacgat
    39301 cgtatacgga aaataatgcg atagtgaaac tataaaatct ggcaaaagat tgctttttgc
    39361 cagatttttt ttataaaaag aggatcaatg tttgttagtt cgaaagaaag gtgtataatt
    39421 taattgaaaa atacataaac agaaagtaga ggacaaagaa tgattatccc aaatagttta
    39481 gaagaattag caggctacgt agaaactgga aagagtgtat ttttctttac tgccggctgg
    39541 tgcggagact gccgttttat caaaccgcaa atgcctgaaa ttgaaaatga cttttctgat
    39601 tggcaattta ttgaagtaga ccgtgacaag tacatagatg tagccgctga atggaacatt
    39661 tttggtatcc caagttttgt cgtgatccaa gacggaaaag aattaggtcg tctagtgaat
    39721 aaagatcgta aaacaaaaca ggaaatcgaa tcgttcctag aaagtgtaac aaaaggctga
    39781 catctgttag gaggggtcaa gatgaatcaa gaatataaga atattttagt aggtatcgac
    39841 ggtagtgaac aagcacttgc tgctttcaaa aaagcggtag aagtggctcg ccgtaataat
    39901 gggaaagtat atgtagcaaa cgtaatcgat cagcaatttt ataactttat gggctatgca
    39961 cctattgatc aaaatctagt cgatcagcaa actgaaacag ccaaggaaat gatcgaagaa
    40021 tgtaaagaat atgggaaatc tgttgattac acaaatatag aaggaattat tgcatatggt
    40081 tctgttcgtg aatccatggc agtcaaattg ccaaaaaaat atgagatcga tctaatcatg
    40141 gttgggcagt caggtctaaa tgcagtcgaa cgctttatga caggaagtgt agccagctac
    40201 gtgatacgtc attcaccttg tgatgtcttg atcgtttctg acgaagaaga acagccaagt
    40261 aattaagcaa gtggaacttg tacgaccttt ttaatcaatt aactaaaaca gtaaagggac
    40321 tgtgacaaaa gtcttgtcac agtcccttgt tacgaataaa tggtgttcca gaagaggctt
    40381 cttcggaaat aaagcatgaa aaaaatttca tgcagtgaat gcaatttttt gttaagataa
    40441 atctcagcaa aggataatgg aggaaagaac atgatttttg cttataacaa agcacacgtc
    40501 ggagacacct tgatggttat tatagccgac gataaaggaa cagaaaacac tgttgaacgc
    40561 aaatggaatg ttgctcggat caaagatgaa caaggtcaag ttgttgcttg gaactttttc
    40621 catatctccg atcatctaac aatcgaaggg aacggccaag taagcttaac agatgaggaa
    40681 ttagcgaaat tgaatcgatt gatcaaagaa gcaggattcg aagaacagct tgaagctgat
    40741 catgaaccga agatcgttgt cgggtacgta aaaacatgcg tcccacatcc tgattctgac
    40801 catttatcga ttactgagac agaagtagac aatggtacag tattgcaaat cgtttgcggt
    40861 gcacccaata tcgaagctgg acaaaaagtt gtcgttgcaa aaccaggagc tatgatgcca
    40921 gatggaatga tgatctggcc aggaactttg cgaggggtag atagctatgg aatgatctgt
    40981 tccgctcgtg aattgcattt gccaaatgct ccagcaaaaa aaggaatctt agtattgcct
    41041 gaggatgccg tagtcggaga agcatttcca aaataaagag gttgtgacaa gtctttgtca
    41101 caacctcttt tttattaatt ttcactattt tgattttgtt ttaatgccga ttgatcgatc
    41161 gtcaattcaa cagtagttgt tttttctttc gagccttcat agtaagtgat tttgacagaa
    41221 tctcccactt ttttcttata caaagcagat tgaagttcta caccagaaga aacttctttg
    41281 tcatcgatct tagtaatgac gtcgtattgt ttcaatccag ctttttcagc cggtgtagct
    41341 gtttggacat ttgtcacgat gacaccgtta gttacagatg aaggaatctt caagatttgt
    41401 tcttgttgct gagtagaaac ggcagataag tcgaccattg tgatacctag agctggacgt
    41461 gtcacttttc cgtctttttc cagttgattg atgatattca ctacatcgtt acttggaata
    41521 gcaaatccca ttccttctac gcttacatta gaagtagatt cagaagtact tgcgatctta
    41581 cttgagttga taccgataac ttgtccttcg atattaacta aaggaccacc agaatttcca
    41641 gggttgattg ctgcatctgt ttggatcgca ttgatattga ctgtttcgtt cgattcattc
    41701 gtacttgtca cttgtcggtt caaggaagaa atgattcctg aagtgacaga atttgcatat
    41761 tcagatccta gtggggaacc aatcgcaatc gcaggctcac caaccttcag agcacttgag
    41821 tcaccaaaag aggcaactgt ttcgactttg tctgaattga tcttcaacac cgctaaatct
    41881 gaataagcat ctgttccaac taattcagct tttaccttcg taccgtcttt catcaagact
    41941 tcaagacctt gctggccatc tactacgtga ttattcgtga ctacataagc agtattcccg
    42001 tcttttttgt agatcacgcc gcttccttca ctataagctt ccaaattact atcatctgag
    42061 ctgctacttt cttcttgctg cccgaatagt ccgccaaaac cgcttgattg gttttgactt
    42121 tgtaagttga taacagaaac gacagcgtct tgaactttat ctaccgcttt ggtgatatca
    42181 gaatctacat tgactttgac attttccaca actgtttcac cggcagaatt ctgattgccg
    42241 cttgtagcag tagaactatt gttgccagtg cccatcgctg cataaaaaat gccagctgtc
    42301 agtagtcctc caacgatccc tccaacaagc cccagaccta acttacgcca gaggctatta
    42361 tttttacttt ttttcatctt tggtgtcaca ttttttctat ccatgtgttc ctcctccaat
    42421 tacattcatt tatctactgt tagtatagtc ttatttgttg actttagtct actttgagac
    42481 tgtgaacttt ttatgaaaaa ctttaggaaa aatcattgtt ttcagatgaa aatatttgat
    42541 tttctttttc cacagatact gtgtaaaact acgcggatag gattggaaaa ataagggata
    42601 aaacatatcc acagcctgtg tataaagtgg ataaattctt ataaaacgta tgtttgccta
    42661 atgaatgtga tgaaataagg taaaataaca ctgttttctg tggataaacc tgtggatgtt
    42721 gtgcataact gttggggaaa tctttttaac aaaaatagcc aaaataaaca tatagacgaa
    42781 aaaattgatt ataataaaca aagtgagcat gaagaccaaa ggagagaaaa catgaatatc
    42841 aaaattatct cagtaggaaa attaaaagaa aaatacctga tccaaggtat caatgagtat
    42901 gtcaaacggc tgaatgccta tgcaaaaatc gaattgatcg aagttcctga cgaaaaagcc
    42961 ccggagaatt taagcgaagc gcaaatgaga caagtaaaag aaaaagaagg ggaacggatc
    43021 ttagcgaaaa tcaaagaaca agaatacgtc tatgccttag caattgaagg gaaaaatcct
    43081 acaagtgaat cttttgctaa acaaatcgac caattaggta tccaaggaaa aagccacctt
    43141 gtttttgtta tcggcggctc tttaggactc agcgaagccg tcatgaaaag aagcaatgct
    43201 cagatctctt ttggaaaaat gacgtatcca catcaattga tgcgtctgat ccttgtcgag
    43261 caaatctacc gtgccttccg gatcaatgcg ggggctccgt atcataaata atgatattta
    43321 agattgaatg ctcctgaaca ctaagttagc ttttcattga aatt
//
LOCUS       DAYYGO010000008        40034 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000008 DAYYGO010000000
VERSION     DAYYGO010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.008"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(51..632)
                     /locus_tag="ACHA5T_000400"
     CDS             complement(51..632)
                     /locus_tag="ACHA5T_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFM8886006.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(638..1897)
                     /locus_tag="ACHA5T_000401"
     CDS             complement(638..1897)
                     /locus_tag="ACHA5T_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFM8886007.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(1899..2945)
                     /locus_tag="ACHA5T_000402"
     CDS             complement(1899..2945)
                     /locus_tag="ACHA5T_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886008.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(3180..3455)
                     /locus_tag="ACHA5T_000403"
     CDS             complement(3180..3455)
                     /locus_tag="ACHA5T_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFM8886009.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(3698..5008)
                     /gene="der"
                     /locus_tag="ACHA5T_000404"
     CDS             complement(3698..5008)
                     /gene="der"
                     /locus_tag="ACHA5T_000404"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFM8886010.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(5190..6419)
                     /gene="rpsA"
                     /locus_tag="ACHA5T_000405"
     CDS             complement(5190..6419)
                     /gene="rpsA"
                     /locus_tag="ACHA5T_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFM8886011.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(6554..7234)
                     /gene="cmk"
                     /locus_tag="ACHA5T_000406"
     CDS             complement(6554..7234)
                     /gene="cmk"
                     /locus_tag="ACHA5T_000406"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFM8886012.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(7283..7918)
                     /locus_tag="ACHA5T_000407"
     CDS             complement(7283..7918)
                     /locus_tag="ACHA5T_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFM8886013.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(7984..9387)
                     /locus_tag="ACHA5T_000408"
     CDS             complement(7984..9387)
                     /locus_tag="ACHA5T_000408"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFM8886014.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(9374..10405)
                     /locus_tag="ACHA5T_000409"
     CDS             complement(9374..10405)
                     /locus_tag="ACHA5T_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8886015.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            10436..10657
                     /locus_tag="ACHA5T_000410"
     CDS             10436..10657
                     /locus_tag="ACHA5T_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFM8886016.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(10822..11559)
                     /locus_tag="ACHA5T_000411"
     CDS             complement(10822..11559)
                     /locus_tag="ACHA5T_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFM8886017.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(11712..13052)
                     /locus_tag="ACHA5T_000412"
     CDS             complement(11712..13052)
                     /locus_tag="ACHA5T_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM8886018.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(13289..14812)
                     /locus_tag="ACHA5T_000413"
     CDS             complement(13289..14812)
                     /locus_tag="ACHA5T_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFM8886019.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(15190..15801)
                     /locus_tag="ACHA5T_000414"
     CDS             complement(15190..15801)
                     /locus_tag="ACHA5T_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8886020.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(15924..16049)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(16131..16847)
                     /locus_tag="ACHA5T_000415"
     CDS             complement(16131..16847)
                     /locus_tag="ACHA5T_000415"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFM8886021.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(16853..17422)
                     /gene="scpB"
                     /locus_tag="ACHA5T_000416"
     CDS             complement(16853..17422)
                     /gene="scpB"
                     /locus_tag="ACHA5T_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFM8886022.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(17406..18176)
                     /locus_tag="ACHA5T_000417"
     CDS             complement(17406..18176)
                     /locus_tag="ACHA5T_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFM8886023.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(18173..18544)
                     /locus_tag="ACHA5T_000418"
     CDS             complement(18173..18544)
                     /locus_tag="ACHA5T_000418"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8886024.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(18563..19450)
                     /gene="xerD"
                     /locus_tag="ACHA5T_000419"
     CDS             complement(18563..19450)
                     /gene="xerD"
                     /locus_tag="ACHA5T_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFM8886025.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIETPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(19546..20019)
                     /locus_tag="ACHA5T_000420"
     CDS             complement(19546..20019)
                     /locus_tag="ACHA5T_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM8886026.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(20124..20987)
                     /locus_tag="ACHA5T_000421"
     CDS             complement(20124..20987)
                     /locus_tag="ACHA5T_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFM8886027.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(21183..23282)
                     /locus_tag="ACHA5T_000422"
     CDS             complement(21183..23282)
                     /locus_tag="ACHA5T_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFM8886028.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(23502..24611)
                     /gene="rpoD"
                     /locus_tag="ACHA5T_000423"
     CDS             complement(23502..24611)
                     /gene="rpoD"
                     /locus_tag="ACHA5T_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFM8886029.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(24635..26476)
                     /gene="dnaG"
                     /locus_tag="ACHA5T_000424"
     CDS             complement(24635..26476)
                     /gene="dnaG"
                     /locus_tag="ACHA5T_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFM8886030.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(26658..28973)
                     /locus_tag="ACHA5T_000425"
     CDS             complement(26658..28973)
                     /locus_tag="ACHA5T_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8886031.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(29158..30018)
                     /locus_tag="ACHA5T_000426"
     CDS             complement(29158..30018)
                     /locus_tag="ACHA5T_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFM8886032.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(30077..30709)
                     /locus_tag="ACHA5T_000427"
                     /pseudo
     CDS             complement(30077..30709)
                     /locus_tag="ACHA5T_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            30876..31637
                     /locus_tag="ACHA5T_000428"
     CDS             30876..31637
                     /locus_tag="ACHA5T_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8886033.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            31663..34254
                     /gene="mprF"
                     /locus_tag="ACHA5T_000429"
     CDS             31663..34254
                     /gene="mprF"
                     /locus_tag="ACHA5T_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFM8886034.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(34442..35629)
                     /gene="tuf"
                     /locus_tag="ACHA5T_000430"
     CDS             complement(34442..35629)
                     /gene="tuf"
                     /locus_tag="ACHA5T_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFM8886035.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            35997..36353
                     /locus_tag="ACHA5T_000431"
     CDS             35997..36353
                     /locus_tag="ACHA5T_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8886036.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(36594..37598)
                     /locus_tag="ACHA5T_000432"
     CDS             complement(36594..37598)
                     /locus_tag="ACHA5T_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFM8886037.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            37764..38615
                     /locus_tag="ACHA5T_000433"
     CDS             37764..38615
                     /locus_tag="ACHA5T_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM8886038.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            38632..38877
                     /locus_tag="ACHA5T_000434"
     CDS             38632..38877
                     /locus_tag="ACHA5T_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886039.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            39064..39768
                     /locus_tag="ACHA5T_000435"
     CDS             39064..39768
                     /locus_tag="ACHA5T_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886040.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT        11302 a         8402 c         6864 g        13466 t
ORIGIN      
        1 attccacatc cagctgacct ttgtcttttt tagtactata ctatttttaa ctaattgtta
       61 tgttcactta cccggttact tttcttttgt gtctgcaaaa ggatctttcg gttaacttca
      121 tcagataatt tcaaaaaagc atccctatct ccatcttcta gtgcttgatc gatttgagca
      181 taaagcattt ttagttttgc ctcttcttct ttttcttgaa aatatcgttc aattaattca
      241 gtatcttctt cactgactga agaatcccaa gaagaaaatg gattgtcttc taagatagat
      301 agatattctc ttgtctgcca agcattttcg aatatacatt ccacatataa aggctttttc
      361 caattcaaac ggatttcatg aaaaatctgg tctgtgtccg taaattcttt tccctgcaaa
      421 aacaatttca tgggttcccc ttcaacagat agatcactga tctgcagtcc tcgatccgca
      481 aaattcgccc cttcaacaaa gtggacattg cttaaaatcg cttcatgatt ggcaagataa
      541 ttcaagatcc aaaaaacttc tcttcggcta aaagatacat gattgaccat ccacgttaaa
      601 aaattcttct tttcccgtac atcaattagc atttttctca cctctctgtt aggtcatcta
      661 ataatgacag tgcctccata tctcctggtt caagtgttaa ataataagtc aataattctt
      721 gcgctctttt cagctgccct tcttctctta agaaaaaggc atactctttc aagaaatctg
      781 gttcatgtga tagttcatga taagcctgtt catagtgtac agctgcaacg gtaaagtctt
      841 ctaattcatt ataagcatgg gctaggttcc attctgcata aggattagca gtttcttcca
      901 tctgattgat cacttgtatc acatcttcat atctttcatc atccaagtaa agattactta
      961 aagtaagtaa tgtttcatct tgtttgtctc ctacttctaa agctttcagt aaaagttctt
     1021 ctgctcgctg tctatcatgc agacggaaag cattttccga ggccaggtga taaagttcga
     1081 cttggtaagg attttctttg attccttctt ctaacactgt ttgtgcttct tcaatcattt
     1141 cttcttcttg aagagcttgt cctagatata gatataacga ttgataatgc ggattaactt
     1201 ctcgaagttc ttccagatag ttgattgcct gctgattttc tttcaattga agattcgtaa
     1261 aagctagctg gaacagtcga tcatctgtcc tttcttcttt cagtgcttct tctaaaggta
     1321 cgatagctgc ttcgaattct ccttgcatac ttaaggattg tcctaaacgt tcctgcatcg
     1381 agattcctga aatttcattt atcccgcttg ccaacaatgt ttcataaacc actgctgcct
     1441 ctttgaaacg atcaactgaa aaatacaatt cagctagcgc aaactggatc aatggttcat
     1501 caggaagcaa cccagctgct tctttcaatt ttgcttcact cacttcaggg attccgataa
     1561 cttggtacaa atcagctatc gccaataagc tttgcggata gtaatcactt tgcttatcca
     1621 ctttttccag gtattcaaaa gctaagtcga tctcattatc ttcgattgca atttctgcta
     1681 acgggatgtt cagtccgtca ttttctgggt attggttgat cagttgttca aaaatctgtt
     1741 ttgcttcgat caaaaagcca atagccagca attcttcgcc tagttcagct aatatatctg
     1801 ctgggtcttc ttttaatgct ttttctaaaa agatttgtgc ttgtgacaag tcttcatctg
     1861 ataaagcttg caacattttt tcgctatttg tattcatttc aattccctct tatctcactg
     1921 acgatttgtt gaaataccgt cattgttctc aataattctt tttcgtttgg atgttggtat
     1981 ccgtctatta tacctatttt ttctaaaaca agtatttttt tgcttttttc tgccaaaaaa
     2041 agaacttttt ccaaatactc catctttgaa atctgttcca gccagtggat tggatagcca
     2101 agcaagtcca gccacacaaa aaagtttttt agttggaaat caaaaggata tgcttggaga
     2161 ttccacacga aatgaaacat cattcctaaa aattttttca tactagcaga aagtagatgg
     2221 ccattttcgg tcagataaaa agcttgttca aacagtttac cataatttga caggttttct
     2281 gcctctaatt gataaaaata gatcatttct tcgatcatcc cggcaaacgg ccgagtcgtt
     2341 aattgctttt tatttggata gtttttatac agattttgca aaaaacgatg atcacaaatc
     2401 attccgcaac agataaaagt caaaaagtca atctgctttc cgtccacctg ccgctcgctg
     2461 attgtttgat cataaacgat gcgttctggc agattgatgg tttgcagcac tacacgattt
     2521 ggtagctgca aaatcgtccg ctgcggtgtt aatgctcggc ttagagaacg gatcgataca
     2581 gggatcatcc atagtgatga atcaagggtt gtatgttttt gaaaaaaacc tgccagatcc
     2641 atgatccctt catttccaaa tccgatgata aacatggatt gattttcggg atagcgtttg
     2701 acgaaatcaa gcaggttctt taattctgtt aaatgattgc attgagtgtt ggcacaaata
     2761 taccagtcat tgtttgcttg atttgcaaat aacgggttga tcttatctgc aaacaaatca
     2821 taatagcgct gattcgttaa aaataagatt ggctgatcaa tggaaagttc tcttagctga
     2881 ctgactagcg tttctccgta aaacactttc gtcaacgcat cttctacttg ataatcaagt
     2941 tccatacgca tccctcctct tcttattttt attttaccat tagtaagcaa attctagaaa
     3001 agataagagc aaagctcacc tcataacttg caaataaata aaaattaaat aaaatacatg
     3061 tgttttctat gtatgacata cggattcgga atctctgtct cttatttatt ccttttctgc
     3121 taggaaataa aaagaaaagc acttgtagcc ctagagccac aagcgctttt tcaactgtct
     3181 tatttaacag cgtcttttaa tgctttacct ggtttgaatg caggtacttt gctagctgga
     3241 atttcgattt cttcaccagt ttgtgggtta cgacctttgc gagctgcgcg ttcgcgaact
     3301 tcaaagttac caaaaccgat taattgaact ttttcgccgt tagctaaagc atcttgaata
     3361 gttgaaaata cagcatctac tgctgcagtt gcatcttttt tagttaaatc agtagctgaa
     3421 gcaacttttt cgattaattc tgctttgttt gccatggata gattcacctc ctccacaaga
     3481 gatatgatag aaatatcatt tagttaaaac tttctgagtg gtccagaagt ttaaaaagaa
     3541 atattgatta acttcaatat tctgttatca cgatatcata tgaaccgctt aatagcaagg
     3601 tttttaccaa ttttcacatg ctttttttag tcatagaaac gtaacaaacg aaactgtctt
     3661 tttcttttga aaaaaaaacg tcgatatggt aaaatgccta ttttctccta cgagggataa
     3721 ttcggatgga cgtcccttca aaagtaaatg cttttctgat ttgattttct aagaaacgtt
     3781 cataagaaaa atgcatcaat tcctcttcat tgacaaaaac gacaaatgta ggtggtttga
     3841 cagcgacttg tgtagcataa aaaatcttca ggcgttttcc tttatctgtt ggtgttggat
     3901 tgattgctac agcatccata atgatatcat tcaacacagc cgaagagata cgaagattct
     3961 ggttcatact tacttgttcg atcatttcag gcagacgttc caaccgttgt ttagtcactg
     4021 cagaaacaaa aacgattgga gcgtaatcaa gataccggaa ttcatcacga atctcttgtt
     4081 cgaaatcacg cattgtattg gtttctttct ttacaagatc ccatttattc acgacgataa
     4141 taattccacg accagcttca tgggcgtaac ccgctactcg cttgtcttgt tcccgaatcc
     4201 cttcttcagc attcagtacc atcaaaacga tatcagaacg gtcaatggca cgcattgcac
     4261 gcatcacgct gtatttttca gtattttcgt atacttttcc gcgtttacgc ataccagctg
     4321 tatctatcat taagaatttc tgaccacttt cagaaacgaa gtgggtgtcg attgcgtctc
     4381 gggtcgttcc ttcaatatcc gaaacaatta cacgttcttc tcctaaaatc gcattgatca
     4441 aagaggattt accaacattt gggcgaccaa tcaaactaaa cttaatcgtg tcgtcgtcct
     4501 cttcttcagt ctcttcagag aaatacttca ctgcttcatc taaaatatca ccaatcccta
     4561 gtccatggct tcctgaaaca ggataaggat cgcctagccc taaggaataa aattcataga
     4621 tatcgtttct catttctgga ttatctactt tattgactgc taaaatcact ggtttattac
     4681 ttctgtataa aattttcgca accatctcat ctgcgtctgt cacgccttcg cgtccgctga
     4741 ctacacaaat gattacatct gcttcttcaa tcgcgatttc tgcttgatgc ttgatctgat
     4801 ccataaaagg ttcatcgcca agatcaattc cgcctgtgtc gatcacgctg aattctcgac
     4861 ctaaccattc accttttgca taaatacgat cacgagttac tccaggagta tcttcgacaa
     4921 tcgagatacg ctcaccagcg atgcggttaa aaatcgtaga cttaccgacg ttcgggcggc
     4981 caacgattgc aattgttgga tttgccatat tatttcctcc tcacaagttt ccaatctcac
     5041 ttagtttaac gaatgcattg ccaactttca agcatagatt gtcaattaaa cgaagtattt
     5101 ttattcctta ttgaaatact aatcgctttt caaaagaaaa actgagacaa aagcagctgc
     5161 tttcatctca gtcacagata caaaatcaat tatttttcag aatcatcggt tgctttttct
     5221 tctacttctt ctttcaacgc atcacctaaa atgtctccca tagtaaatcc agtattttct
     5281 tctggtaatt cataagactc aacatttttt ggttcttctt ctgaagctgg tttttcttct
     5341 aaagctttga tactcaatgc aatacgatgt tcttctggat ggatctctaa tactttcact
     5401 ttcacttcgt caccttcatg aagcacttca tgtggtgtcg caatatgttt atgtgaaatt
     5461 tgagagatat ggactagccc ttctactcca gggaacactt caacaaaggc accgaagctt
     5521 gtcaaacgtt taactgttcc gtctaatact gtgccaactg gagcttttgt atcgatttct
     5581 gaccaaggtc caggctgtgt gtctttgatt gacaaggaaa cacgttcttg ttcaggattg
     5641 atagacaaca ctttgacatt tacatcatca ttgacagcca atacatcact tggttttgct
     5701 acatgactat gtgaaatctc tgagacatgg actagaccgt caattccgcc taggtcgaca
     5761 aatgcgccaa aatcagtaag acgtgcaaca cgtccactga gaacatctcc ctcatggata
     5821 gttgaaagaa tttctttttt cttttcttct ctttcgtttt ccactaccgc tttatgcgat
     5881 aagatcaaac gattttctga tggttcgatt tcgatgattt taaagctcaa tgttttgcct
     5941 ttatagtctg aaaagtctgc aacaaaatga tcttccacca ttgatgcagg gacaaaacca
     6001 cgtacaccga cgtcaacgac taaaccgcct ttgacaacat ttgtaacagg tgcttcgatg
     6061 accttgccct cttggaaatc tctttcgata tcttcccaaa cttttttagc atctaaacgg
     6121 cgttttgaaa gtagatagct gccgttttct ttatctttgc cgatcgtact gataacgact
     6181 aaatctaata catcaccgac ttttactact tcgctgatat cttctaccgg caaagttgat
     6241 aattcttttg ccggaacgac tccttcaact ccagcccctt cgattccgac aatcacttgt
     6301 ttgtcttcaa tgactaaaac ttcgccctta acaacatcgc caacttttac ttcttgtacg
     6361 cttttcattg cgtcttccat tgactggttg ttctcttgat tttgatcaaa ttctgtcata
     6421 acacttgtcc tcctaaccaa cattctgccg taagtacggt actgttttta gtttaattga
     6481 ttattcaaaa aaaataaagc gatttcgttc gttttccttg aaaaaacaat tgtttttcat
     6541 tttttttctt aatttaaaaa agaagataac cttttttcaa aacaacagct tttattgctg
     6601 caacgacttc tggaatcgat ttgccagtcg tatcgatacg aacagcatcc tctgcttgct
     6661 tcagcggaga gacgtccctt gttgaatcat aatggtctct ttcctgtatc tctttcgtca
     6721 gtgtttcaag atcagtcgga attccttttt cttggttctc tttgaaacgt ctttctgcac
     6781 gctcacttac gcttgctact aaaaagattt tcacttctgc attcggtaat acagctgtcc
     6841 cgatgtctct tccgtccatg acaacgccac cgcgttcccc tatttttcgt tggacagcaa
     6901 ctaattcttc tctgactttt ccatgtgcag agacttcaga aacagctttt gtgacatctg
     6961 gttgacggat ttctaaagtc acatcttttg atgctatgaa tacatgctgt ccatcttctt
     7021 gttgcgaaaa agagatagga tactttttga tcagcgaaac tagcccttct tcatctgaaa
     7081 aagatacatt gttcgtaata gctaaataag ttactgcgcg atacatggca ccggtatctg
     7141 tataaatata attcaattct tttgctaata tcttggcaac ggtacttttc ccagatgaag
     7201 caggcccgtc gattgcaata ctgatttgtg tcatgatata actccttttt ctcagaaaaa
     7261 aagccgcaaa gcgcggctat tattatttaa ttcttaattc ttgaccaggg taaagcataa
     7321 agttgtttgg atctaagcca ttcaattcaa acaattgatc agtggtgatc cctgcacgct
     7381 cagccacttg ttttggacct tcgcctgctt gaacagtcgt tgttgaaccg gctgcagctg
     7441 atgaagaagg tgttgtttct gctgcattgc tgcttgaatc ccctgcttga taagttgcct
     7501 cacttgagga agtcggtgtt gtctctgctg caatactgct ttctgacgtt acacctgtat
     7561 cttgagacac agtagattct tccgcgattg tactagattc tactgttgaa gtctcagttg
     7621 actcctcttt tgttgtactt gtagaacttt ctactactga tgtcgaagtt tcagaagaag
     7681 ttgctgctgt tgcattgttg cttgatccat ttctgatcca gaaataagca cctgctggaa
     7741 tagcgataca taagaccagt aaaatcaaaa atagcgaaag aaataatgta tttcctttct
     7801 tttgttgtct ctgagaactt cttgatgaaa cttcatcatc tgtatcgtag attggttgtt
     7861 cccatggttc ttgtgtttcg ttattttctg tagattgatg tctatctttt ctgctcactg
     7921 ttttacctcc taaactctat cgtgtgtatt ataccatagg aaaaagtgtc ttgtcctaaa
     7981 aaatcaatgt tccatgtttt ttttaaataa atttagtaaa atcgttcccc aatgaagttg
     8041 ttcagtctta gaaaaaagct tcctctttgg cagaagttct gcttttgctc catcaatatc
     8101 acaacaattc tgcggctttt cggagagttt ttcgccaaaa taagctaaga tgaattttct
     8161 gcggcagttt tcttcattaa tatagcgaag catctgctgc aggcggaatt ctttttcttt
     8221 ttcctgacgt ttgagccgtt ccacccaatt ttctggtttt tcagactgct ggatcagctc
     8281 catccatttt ttttgcagct catcaaaggg agcttgctct gtggcatatt ccaaataaag
     8341 ctcaaaactt tgtcgctgtt ctctagaaag ttgattaaag aaataatgga tacgttcatc
     8401 cccactttga taaaaaagaa ttgctgcact ttcttcctga tctctgcctg ctcgaccgat
     8461 ttcttgtaca tagttttcta gactgtctgg caaatcataa tggaccacat aacggatatc
     8521 agatttatcg atccccatac caaatgcatt cgtagcaatg agaaactgca gctgattttt
     8581 tacaaactgc tgttgaagtt gtcttctttg agctgcatca agaccaccgt gatagtatcc
     8641 aactgtaaaa cgttccctga ataaatgata caatcgttcg acttcttttt ttgttgcaca
     8701 ataaatgatt gccgctcctt gtgctctttc catgaagtgt tcaagttctt gttctttttg
     8761 ctgcgttttc cgaacaaaaa gcgagatatt ttgccgattg actgattgtc tgatctcttt
     8821 tggtggtcga tcaaatagta catgacgtat atctcttgct actaaatctg ttgctgtcgc
     8881 cgttagtgcc agtgtgaccg ggtttcctaa aagctttcgg atttttccta actgttgata
     8941 ttctggacgg aaatcaactc cccattgaga cacgcaatga gcctcatcta ctacatataa
     9001 cgcaatattt tgttttttca gatgctctaa aagagaagga ttagtcaaca tttcgggact
     9061 caaaaacaaa aatttatatt gcgatagatg acggaggacg tatcttcttt cagattgcga
     9121 aagtaaacta ttgaaagcag ctactcgttt ctcccctcgt ttttgtaatt gcgtcacttg
     9181 atcttccatt agagaaagca gcggtgacac gatgatgacc agaccttcca ttaaataccc
     9241 cgtcaattga tagcaaaggc tttttcccgt tcctgtaggc aaaatagcga gagtatcttc
     9301 tccatcaagc aacgcctgaa taatttcttc ttggccggga cgaaatgaat caaatccaaa
     9361 atattttttt aattcattta ttaacattct gtatcgcttc ctttctcaat agatagattt
     9421 gatacaaacg aaattctcca taatctaatt gtccatctac attccattcg ctaaagcgcc
     9481 actctcgcac ggaatcctta tttggttcca aacgttcgat tgtttctgca gagagaatcc
     9541 actcgtatgg aaaatcatca aaaaacaagg cccactcaat aagatgatct gttactgttc
     9601 ctctttttaa atgacggatt gccatgatct ggtcaattgt ctgaccattc ataaacatct
     9661 gcttggtgat catcatgctt tgattgaaat tttgctgtaa taatgaatcg atcaaagcgt
     9721 gccaaagcga atctggccgt ttttcgattt gtgctaacaa aaggtggata catctgcttt
     9781 gttgcagata aaccgctgac tcatcattgt tttttgccag ctgataagga agcagccctg
     9841 ttcgctcatt tccagataat tggttagcta aaaaatctgc tgcttcttct ggtatctccg
     9901 aaaacagctg caccaaactc tcatacgcag tatcaatcaa aatttcacgt ggcaattggc
     9961 tttcactaag ccattttttt agttggaaag tataaaaagg acttgtctct gctggaagat
    10021 agtcttgaat acccgttgcc agattagaaa ttacttgaac agcaaattta atgagccgcc
    10081 aactagtttc ccctgttctg ccatagcggt caaaacgcaa accagtatgt tctatttgat
    10141 gttcggacaa catatcagcc cctgcaggcg ttagctttgc ttcattttca tttatttcga
    10201 tccaaccttg ctgcgcaatt ttttgcaaaa tttgataaaa cttatcctgc ttcaattctg
    10261 gaaaactgtt atggataaac aaaagttcgt gggtaaatcc ataaataagt actgaagtcg
    10321 ttcgttttcc cacaaggagt tggtaaagag aactaatccg cagcttgtgt cctgatgaaa
    10381 atagcgataa aataaaaaca atcatattat tctccttaag ttaggatggt tagaaatgtc
    10441 actactatgt aaacttgtac ctgaacgctg tattgcatgc ggtctttgtc aaatcgaagc
    10501 gccagatatt ttcgattatg atgaagaagg aatcgtttta tttgcccaag aaccagaggc
    10561 tcatcaacag ttcgtgatgc cggaagaaga aacatatgta aaaaaagcgt atcaaaaatg
    10621 tccagttcga gcgattttat tagaaaataa aatataaata taaacgaaac tttaaaaacg
    10681 tacactaagt tctctaaact tgaaaataag ccgggatctg tttctcttgt tccgtgaaat
    10741 cccgacttat tttgaaagat tcatttgcta tctatagtta attgactagt tttttcacgg
    10801 accctatagg acccatttat attatttcaa ctttttcagc cagttttcca atgtattgac
    10861 gatcacagtt tgctggtctg cgttagaaat ttctgcctct ccatctcctt tttgctcacc
    10921 atagctgcca aaacctccat gatttccgcc attgattgat acatactctg ttctttccgg
    10981 taaatactgc ttagcttctt gataagcttc ctgattcaat actttatctt ttgtcgccgt
    11041 gatcgataaa acaggaacag ctgattctgt caatgttcct tttttgtcgg gataacttgc
    11101 catgaagaat acccctttta aatgtttgtc tgaatgattc ttattgacat aacgacttgc
    11161 cattacgcca cctaatgaat ggccaccgat cacataatcg gaatttggaa aatcttcttt
    11221 gaccttatcc gctttgtttc cgcccatcac tgctagatca agtggcattt tgacaatagc
    11281 gactgaaaag ccgttttttg ctaaatcttg cgcccaaata ctataacttt ctggctctac
    11341 gagagcacca ggataaaaaa taagtaatgg agactttcca tgattctttt tctctccttc
    11401 aaaaaataag taatcttctt tgttttttcc ttcttttacc aacacttgcg cttctgacga
    11461 tggttcgtac gtattttgct gtatatagta ataaccggct ccaccgagta atcctaaaat
    11521 gatgactaag ccaattagta ttcgtctaaa tattttcact ttctcccacc ttttctatct
    11581 gagtgacccc tttttcttaa tcaagtatga ttatacctta aaaaaataag agagaaatgc
    11641 aaactttcca gcatcaaata aaaaaagacg gaatatccat ttgaatatcc gccctttttg
    11701 tatgtttatt tttatttagc acataaacga tacacagctt ctgcataaat ttccattgct
    11761 ttatacatac tacttaattc ccatcgttca ttcgcctggt gcataaagtc aggagtatca
    11821 gggaacatcg caccgaatgc cacacactgg ttcatcgtac gagcaaaagt agcgccgcct
    11881 gatacaaacg gttcagtcat gtctccagta atatcgcgat atgtccccaa tagatttttc
    11941 actaactcac tgtctagtgg cacgtataat gaatcaagga agtcaaattc ttcataagtc
    12001 aacccatatt tttctgctgt tttagttaat ttagctacta aatcatcttt cttgaatgta
    12061 acagggattc tcatatctac accgattttt gtttgttctg gtgtgatttc caaacttgca
    12121 aagttcatcg ttagctcgcc ggattgttca tcttctgttt tgcccacgac agcttcacca
    12181 gttgcatttt cttgaactaa ttttcctaag aaattgattg ggccaaaatc aaatacttct
    12241 gaaagagcaa ttgccagacg agaaatagca ttcactcctt gtgctgcatc tttcgcgtgg
    12301 atacttttac caagaacgac gatgccttca ccttgttctt cgaaatcgaa ttcgtgtttt
    12361 ttcaatgctt cttttacttc agaaagcttt tctccagagt aaggagctgc atcaggaaca
    12421 acattcaacg ctcctccagc ttttacagaa aactcgttcg ttcctggtcc tgtcaagtaa
    12481 gcttgcaata agcctttttc tgcataaatt aaaggaaatt cagcatctgg cgcaaatcct
    12541 tgcgtaattc cttcttcttt ttcgttatat ttttctaaac aacgccataa attttcttca
    12601 tctgtaccaa agataaaacg aatacgtgtc ttgaattcca cgccagcatc aatcaaagct
    12661 ttgacggcat acattgccgc aatagaagga cctttgtcat cttgggatcc gcgaccaact
    12721 aaccagccgt cttttatagt tggatcgaat ggttttgttt cccaattatt ttcgtcccct
    12781 gctggaacaa cgtccatatg acacaagata ccgaataatt catctccaga gccaatctct
    12841 gaataaccgt aatagccttc tggatcttta aatgttctaa atcctagctt ttcactgatt
    12901 tccaatactt tatccaaagc ttcaatcact tttttaccga agggatgtcc ttgtcctgaa
    12961 tctgcttcat ctagtacgga aggaattcgg atcaattcgc tcaatgattc taatgcttgt
    13021 tcatgatgtt gttctgtcac aaattttttc atgatattat cctcctaata tgttaaaaag
    13081 ggtcatgtca aattcctccg acacaacctc ttcttctcaa ataatgacgt tttaagcctg
    13141 ctccatcagc aagcagaatg ttcttaacaa atgatgaaag aaaaaacact cactgaatgg
    13201 agctattgac ttatccacgt gcttatcgat agccgtttat cggtttcttc attcttatga
    13261 tagaccttgt acctgtattt gtctaggttt agaacattgc tgctacacct aggatcacac
    13321 agatggctac aaataatatc gcaattaatt taccggtaaa tttccaccac actgaaacat
    13381 cgaaacgagc aattgctaca gcacccatta cgatagcaga tgttggtgta attaagttta
    13441 ctaaaccaga tgcagattga taagcagtga ttactaaatc cttaccgacg ccggcaaatt
    13501 cacccatagg tcccatgatt cccattgttg cagcagctaa gccggaagtt gaaggaatca
    13561 agaatgacat agggatatag aaaataaatg taataatgat gaatacactt gaagataatg
    13621 aacttaatcc ttgttcgccc caatgtaaga tggtgtctgt gattaatcca ttgttcataa
    13681 ccacttggat accacgagca acagctacta cgattgctac accgatcaaa tcagaagcac
    13741 cagctaaaaa ttcagagaca aattcagact ctttcatgcc ataagtgatt gctactacga
    13801 tggacattac taagaataac atagtaattt ctgggaagta ccagtcacct aatggaagca
    13861 tgcttttacc tagaaccgta cctagtaccg gaaggtttgt taaccagttt gtcaatgatt
    13921 caaaaatcgt gaaattcgca ttcaaggttg tccaaggaat caaactgatg atcatgatac
    13981 caaaagtaat aaagaacaat acattaacat gtttttgttt tttcgtgatt acttcttgac
    14041 gaccgattgc ttcaatatcg aaatgcttca tatcttcttt acgttggttg tatactaatg
    14101 attttgttgg atctttttcg atctttgatg cataacgata cacatataca atagaaatag
    14161 ctaacaaaat aacgaacatc aagaatcgaa gaccaatacc ttcacccatc gaaatcccaa
    14221 cagcttgtga agctacgcct gttgcaaaag gatttaccgt tgaagctaga cagcctactt
    14281 gagaccctac taatacgatt gctacagcta ctactgtatc aaagcctaca gccatcatga
    14341 ctgggatcag taaagcatag aatggaatcg tttcttctgc cattccgtaa gtagagccgc
    14401 ctaatgcaaa taacagcatc aatactggaa taagcatttt ttcttttcct ttgaatttct
    14461 tcacaactga agcaatcccg gcatctagtg cccctgtttt gttgataata cctaaaaatc
    14521 caccaacaac caaaatgaag aaagagattg ggattgcagc aggtgtttct ttagttagct
    14581 cccccgctgg tacacctagc atcccattga ttggcgccat gaaaatatcc cacagccctt
    14641 gaggattttg ttgtactcgt tcatatgatc cagcgacgat gttgcctgca tcgtctacac
    14701 tatagtgccc agctggtata aaccaagtaa gtacagcgat tactgcaata ataataaata
    14761 agaccgtata cgctgaaggc atttgaaatt ttttcttttt tgtttgttcc atcttcttct
    14821 tcctttcttt tccgttagag acaacaattt tggctctaat gttattttat caaaaagagt
    14881 atcagaacaa aaataataac acgcattttg taaaactttt gttcaaaaag ttcgtttttc
    14941 cctattccct cctctttttt caggttttta aagctttctt ctttttcatt gtagcgtttc
    15001 ctttttttta taaacaaaca ttttcgtctt ttaaaaggag aagaatttca catttgcaaa
    15061 gaattgattg atttttatgc ataaatcaaa aaaatactca aaacaaacaa ataaaacgtt
    15121 tacattattg catgtcttgt atttttttcc aaaaaaaaag accaagaaat ttcttgatct
    15181 ttttgatatt tatttgactg ctgtatgacg ttgttcgaac tgatgttgtt ttcgacttaa
    15241 ccatggaaga agttgcgcat gcagcactaa aaagacagca ctcacgatga atcctttgat
    15301 caagttgaaa ggaacgatcc caatcagtac atagtttgct aaagacatgc ccagcatctg
    15361 tgttgcgttc agccctaaaa acattaagta aagaggcgta ataacgaaat aatttgcgat
    15421 actcataaaa atagttagag cgatggttcc tgtaatgaca cctaaaaatt tgtttcttcc
    15481 tttatgctgt ttctttacac taaagaaata ataaatcggg aatgtaaaga tcgttgttga
    15541 taagaaacta gcagtgtctc ctaccaaatt atccggagaa aaacctgtag tcagcagatg
    15601 taatactgaa cgcaagaacg ctgttgcaac tccggccgct ggaccgaata agaacatgct
    15661 gatcaatacc gagatatcac taaaatcgat cttcaaaaaa ctaaaagccg gcatgatcgg
    15721 aaaagcaaaa tactgtaaaa ctaatgcgat cgcagcaaac atcgctactg caaccatttt
    15781 ctgtacacgg gtgttcttca tttgaaatcc tcctgttagg actcctcttc agcgaacttt
    15841 ttgacgtttc tctccaaatg aaaaaactct attttccagg gaaaatagag ttagagttat
    15901 ttgatcaagc gtcgtcaaat aagctctatc ttctttatcc agactatact gtcggtatcg
    15961 gaatttcacc gattcagcta tttttttgca aaataggtcg tggactataa ccaccggtcg
    16021 ggaatttcac cctgcccctg aagacgaacc ttatataatt tttgtttaca tattcattat
    16081 acatatctat tcaaaaaacg caacatctaa cacttataaa aagtaagtgt tcattttgag
    16141 tggttcagca aactgctgac ttcttttttg ttcagttcac ggtaatcccc aggacgtagg
    16201 ccttgtaaag tcagatctcc gtatttttca cgttttagtt tttgcacagg aagaccaact
    16261 gcttgaagca tatttttcac ttgatgatta cgtccttcgt ggatcgtcaa ttccacgata
    16321 ctgtggtttg tcttaggatc cactgataaa atttggaatt tagctggaga tgtttttcgt
    16381 ccttcgatct ttattccttt tgtcaaaggt gcaagcatcg attttgtagc gatccctttt
    16441 acctttgcaa cgtaaacttt atccacttca tgttttggat gtgtcaggcg ctgagcaaaa
    16501 tctccatcat ttgtcagtag caagatcccc gaagtatcgt aatccagtcg acctacagga
    16561 tagatacgtt cttttaccat cggcaaaaga tccgtaacaa cttttcttcc tttgtcatct
    16621 gcaacagcag aaataactcc tttaggtttg tacaataaat agtaaccgta ttcttcttga
    16681 taaattggga cttcatccac ttccacagtg tcatgtttcc caacttttat ccctaattcc
    16741 cttacaactt gtccgtttac ttttacacgt cctgcaagga tgaattcttc tgcttttcga
    16801 cgagaagtga tgccagcgtg ggcaattact ttctgcaatc tctccattac aattattctc
    16861 ctttattcat tccatcaaag aatagatctg gtgacacgtc ttcttcaagt gaaatttcca
    16921 tttcttgtgt atcaggcagt tcttctaatg atttcaaacc aaaataatct aagaagtagc
    16981 cagttgttcc atataatatc ggccgacctg gtccttctac tctacctttt tcttcaatca
    17041 gtttatgggc agctagacgc tgtaccgctc ctgaaacctg aactcctcga atctgctcga
    17101 tttcagctct tgtgattggc tgtttataag cgatgattgc taaggtttcg acggcggctt
    17161 gagataatgt gtttgatgtc ccttgcgcat atcgcttaag taagggagcc agctcttttt
    17221 tagtcgttaa tacataatgg ctttccgttt caaaaatatg caaagctgag tgcgagtttt
    17281 gagtgtaaga ttcttttaat tctttcacta aacttgtgac ttctgcttcg ctgagattta
    17341 acagtccgct taattcttca ttactgatcc cttcctcgcc aacaataaaa agaagggctt
    17401 caagttcact taatttcgtc atgttcggta tctcctactg ctggataaag cataattgat
    17461 tcatagtttt ctttttgttg cgcaatgatc tgcccttttt tcatcaattc tagcaaagcc
    17521 ataaaagtag tgacaacttc acttttagaa ggtgactcaa aaaaagaatc gaaagtacag
    17581 ccatgcgcat ttctttttat acgctcttcg ataaatacca ttcgttcttc gatcgttgtg
    17641 tcatcgggcg tgatcgtttt ttccactttt tgagaaaatt tcttacgttc taagattttc
    17701 tgcatggcac ggatcaaatc gatttttttg acttgtcctc gcttcaacgg cttttcagca
    17761 tccgctaacc catcgatgtt cataggttct tttgtgtaat aaagacttcg ttcctctgct
    17821 ttttctgtca gtctttcagc tgcatattta tatttacggt attctaaaag ctgagtgacc
    17881 aattcttctc gaggatcttc ttcccataat tcttcatcgt ctgtgacgaa ttcttggaca
    17941 ggaagcaatg tcttgctttt gattgccatc aatgtagcag ccatcactaa gtattctcct
    18001 gcaacagcta gctcgagtgt tttcattgta tggatataat tcatatattg ctccgtgatc
    18061 gtagcgatcg gtatatcata tatatcgatt tccatttgct gaatcagatg aagcagcaga
    18121 tcgagaggac cctcgaatac atctaattta atattgatct ccgtcaactc attcactctt
    18181 tcttcttttt ctttgctagc cattttccaa tgtaaggctc tgtgatttca gttgcacgga
    18241 tggagacatc cgggtacttt tcctgtaatt catctaacat ttgaaagccc atattctcac
    18301 cgcgataaga cggattcaaa gaaatgtcat ggatgatcag ctgatcgttc gttgcccagt
    18361 aaataccaat aatcccttga atgttggttg aaccttctgg cgtaaagaaa aaaagttcat
    18421 aatcgtcttc agattcgtac gtatcaatct ccttcaacaa agaagtttta cctgtcagtt
    18481 tcttatggaa actcaatagt cccatagcaa ttttacggtt ttctttccgg taatgtgtta
    18541 acatcttctc accttcctat gactaagccc ttggaaaatg ctgcttataa acatccgtca
    18601 ttcttttctt cgtgatatgt gtataaatct gagtcgtaga gatatccgcg tgacctaaca
    18661 attcctgtac tgtccgcaaa tcagctccat tttctagcag atgtgtggca aagctatgcc
    18721 gcagcgtatg tggtgttact tctttgtaaa tcccagcctc tcgtactaac tgcttcagat
    18781 ttttccagat tccttgccga gaaagacctt tcccatgatt attgacaaat acatgcattt
    18841 cagatgcgtc ctttacaagt aatggacgtg cttcgtctaa gtagcgttcc agccattgga
    18901 tcgcataatc acctaaagga atgatccgct ctttatctcc ttttccaact gtctgtacca
    18961 gtccaagaga aagatgaaga tctcccaatt tcaatccaat caattcactg acgcgcatcc
    19021 cagtagcata catgacttcc aagattgcac gatctcttat ccctaacgtt tttgtcgtat
    19081 caggtgtttc aatcagcttt tctacttctg tcagagtcaa tgtacttggt aatttctgtg
    19141 ctttcttcgg cgtatcgata tgttgcatcg gatcatgatc agtgatacgc tcttgccgta
    19201 aaaactgatg gaatcttctg agacttgaga tcatccgagt aacagttgct gtcgcttttt
    19261 tttcttcatg caacttctcc agaaaacgga tgactaaaaa gcgatcgaca tcctgccatg
    19321 aagcaatttt ctgctcctgt agaaaatgca agtactgttc caaatcacgt tcatagcttt
    19381 gtcgtgtatt ggcggacaat ccccgttcaa tattcaagta gtggagataa tcgttgattt
    19441 gctctttcat aataatcccc ctcatgcctc tctatcatat cagataccgc ttcattctga
    19501 tagtcttttt ccataatctg agcaaagaaa aagccgtcta gacactcaga cggactggtt
    19561 tcttttttct ttttcatagc attcctgaca gattccatgg aacgttagcc gatgatcttt
    19621 tactaaaaaa tgataacgtt cttctactac ttgctccact tcaagtaaaa gatcctcttc
    19681 tacttcctcg atcgtgccgc agttcaagca tagtaaatga tgatggaaat gtttggcgcc
    19741 ttcttttctc aaatcataac gggctaatcc atcattaaaa cttactttat cgacaatttt
    19801 caattctgtc aagatttcaa gggttcgata aacagtagca agtccaattt ccggactctt
    19861 ttttttcact aaaaagaaga tttcttctgc agataaatgc tctttctcat tttccaaaag
    19921 cacaagcaga gtagcttctc tttgcggtgt cagcttaaac cccgcttcat gtagctgctg
    19981 ttttgttttt ttcatcgccg aaatagcatt catgaccatt tactcaaccc caacaattta
    20041 taataattac aaataaaaaa agatataaca ctatgttaat taaaattatt ataagctagt
    20101 taagaaaaac atgcaaggga ttctcaatta gattttttga ctagttttgt cacaccgttt
    20161 tcttgtgtaa tcaatccttg tttcaaaaga ttacctaatg cgcgcttaaa tgctcctttg
    20221 ctgataccaa aagctttcat gatttcttct ggatccgatt tatctgtaaa gttgatttgc
    20281 tgatctgcag aacgttctaa aatggttaaa atcatttgtg catcatctga tatagcttca
    20341 tatgctcttg gcttcaatga aagattcagt acaccgtctg gtcggacacc aatcactcga
    20401 ccttttatct gctcgcctag acgaggttca ttatatcgtt cagaaggatg gataaaccca
    20461 agatagaaat cttccgttaa aacgtacgta cctgttaatt tcaagcggta tgctgttcct
    20521 atcacatctt tgtttttcat ttcttctgtt ccatatttgc ttaaagattt gaatattttt
    20581 tcatctgcta aactgcccca aatacggtct tttgcatcaa ctctaaggga aatcatcaag
    20641 cgatcccctt ttttaggcca tagctctgtc attgtcggca attcatcaag agatacgaca
    20701 aagtctttat ctggaagacc gatatcgaca aacgcaccta aatcttttcg tgtagcagta
    20761 acgattccaa aagcgtaatg accgatacgg cttttaggaa tttgtgtagt gatccgattt
    20821 tcatgttttt gattttggta accgaatcct tcaactgctt caccgatttt gtgttcacct
    20881 tctgatttgc ttaagccaaa ggtttgtccg tttttttgga caaagtagtt tttttcattt
    20941 tcatcaataa taatacctgt aaatatagtt gctaataaat cgttcatttt tttctccctc
    21001 tatagtgaaa ggttttctta atacatttct ttgctatcat aacataatgt tatctacttg
    21061 aatagctgag ccttggtttg gaaaggtgaa aaaaggagta tgacaacagt tgtgtcatac
    21121 tccccttttt cgaataaacg gtgttaaacg gcaggctcac agcctctttc atttgcctaa
    21181 aattaattgc tcccatttcc tccgaaccat ccagttttac agttgatggt tacgatgtaa
    21241 aatggcaaag cactttctcc aatgcctttc atcaatgcag attttgttga agtggcatat
    21301 agattataat agccttcccc attttcgatt ctggcttttt ctaatctgta acctccttca
    21361 acgatatatc cacgttggat acatgtagca atcactttgc tttggacacc tggtgcccac
    21421 gtccatgctg gattagatgc atcggcaata tcacttgctc tggcagattt gattggctga
    21481 ttcgctgagt ttgaagagga aggggtcgag gatgccgctt cttgctgtgc tgcttgtgcg
    21541 accgcagctt gttgagcggc ttgagctgca gctgctgctt gagcggcttg ttttgcttgt
    21601 tcttcagctg cttttttcgc ttcttctgct tttttctttt cctcttcttc ttttttctct
    21661 ttatcagtga gcatcttatc tacttgtttc aattgttcat tcaactgagc agatagatct
    21721 gtatttttca ctttttctac tgcttctttt gccgtcttgt attggtcgcg tgttgctgaa
    21781 tccactactt ttccgtcttt aaagactata gctgtttttt ctttagctgt ttgcaattca
    21841 gcatattgat ttttagcttc tgtttgtgct tgttgaatca attttccaaa atctgtgtta
    21901 tccgtttcga ttgcttggat ctctttatct gctttcaact tgatgtcatc tgctagatga
    21961 tcatcgacga tggcaggtgc tatgaaccac tcattcactt tttggatatc tcggatctta
    22021 gattggatat cttggtaagt tgtttctaac tctgaatatc ctttttcatt cttaagactg
    22081 gataaatcat tttttagttt tgataaatct tgatgaatca tatctgcacg aataaattga
    22141 tggtgatcat ctgtataaaa ggcagcaagt ttctcatgaa gttcttccat ttgttgctgt
    22201 ctttttgctt ctgctgctac ttcggcttct actttttgat gatggtcata ataagcccag
    22261 cttcctccac cgagtagaag tactgctgct aaaacgagat aaggtacttt ttttgatttt
    22321 ttcttttgcg ctttatctgg atctgttttt tctaagtttg attcttgatg tgttttttca
    22381 gtatctggca cgattgttat tgatttcgct tctgcttcaa acagcttagg ctggttctct
    22441 tctttttcta tttttgcttc aagatcagta ccctctttgc ttacagattg attttcagca
    22501 tcggattcac tatttatttc atcgggctct gttgactttt tgtctgtgtt ttcgagtatt
    22561 tcttttttct ctgcttccgg cagtttattc gttttttcct tttctaattg tttttcagta
    22621 aatacttcag ttttttctgt gatatccgtt tttacgtctt ttatttttac tgtttcttta
    22681 cctgtggcgt tttctctatc agagtctttt tcagtttctt gttccttttc tggctctact
    22741 ttgttttcct gtgcaatttt ttcactgatc attttgagca gctctttcgc gtgctcttct
    22801 tttgtatctt cacgatgtgc tttgatataa gcggatagta tcgaattttc ttcctcatta
    22861 tcgacgattt ctttcagttc agctactctt cttttttgtt ttgattcaag cgtttcttct
    22921 tgattctttc ctgattcttc cgaagtctca tgaacagttg ctttcttctg ttcatttttc
    22981 tctatattag ttacttgatc gtgagcaggt ggttcttcgt cttctggttc tgctttttcg
    23041 aataattcca ttacagattc tagaggtaaa tctttcaatt ccgaccattc gattggatcg
    23101 tttaattctg tatcaggaaa gactgcatct tcagttgatt tatcctgcat agctgcatct
    23161 gtatttgttt cttcaattga ttcttttatt tgctgatctt ctgcagttat ttgatgaccg
    23221 cagtctggac agatagtatt ctgctccatt tttttcgatc cgcaatttgg acattttttc
    23281 atcacttagc gatcggctcc tttactaggt ttacgctacc atttaatcta tcatattcta
    23341 tacgtcacgc caatagctta tttttaggat ttacttaaaa ataatgaatg aatttccttc
    23401 ctatagaaaa aaaagcttga gactgcgtag tctcaagctt tttttctttt aaaataaacc
    23461 aaacagtttt actgatttct cttctggtca atagtccacg cttattctaa gaaatctttt
    23521 aattgttttg aacgagaagg atggcgcaat tttcttaatg ctttagcctc gatctgacga
    23581 atacgttccc gagtaacacc aaatactttc cctacttctt ccaatgttct cgtccggcca
    23641 tcatctaaac caaaacgtaa gcgaaggaca ttctcttccc gatcagtcaa tgtatctagt
    23701 acatcttcca attgttcttt tagtaactca taagctgcgt gttctgctgg acttgttgct
    23761 tcctggtctt ctatgaaatc acctaagtga gagtcatctt cttcaccgat tggcgtttca
    23821 agggaaacag gttcttgcgc gattttcaag atttctcgga ctttttctgt tggaagatcc
    23881 atttccgcac cgatttcttc tggcgtaggt tctcttccaa ggtcttgcag caattgacgt
    23941 tggatacgta tcaatttatt aattgtttca accatatgaa ctggaatacg gatcgtacgt
    24001 gcttggtcag cgattgctcg agtgatggct tgacgaatcc accatgtagc ataggttgaa
    24061 aacttaaatc ctttgcggta gtcaaatttt tcaactgcct tcatcagtcc catgtttcct
    24121 tcttggatca agtcaaggaa ctgcatgcct cgtcctacat aacgtttagc gatacttact
    24181 actaaacgaa ggtttgcttc tgccagacgc tgtttcgctt cttggtcgcc ttcttcgatt
    24241 ttcaatgcta acgctacttc ttcttccgca gttaaaaggg agacacggcc aatttctttc
    24301 agatacatac gaaccgggtc attgattttt acaccggttg gcgcagaaag atcttctgtt
    24361 tttgcttgtt cagctttttt ctcagcactc ttcaagctgt gaattgatgg atcaccattt
    24421 tcatccacca cactgatacc tgcgtcttca atcttttgga tcaatttgtc catttcatct
    24481 gcattcaaag tgaatggtgt agcaagctga ttggataaat catcatatac aacttgtcgt
    24541 ttgggtttgt tttctttgat gaaagctgct actgctgctt catacgtttt tttatctgtt
    24601 ccatttgcca taaaagaagg ccccccttga gtagctattt ggcttgtttt aactgtttcg
    24661 ttaaattgat gatttcaatc gtcaattcaa gctcaagctg tttgtttcca gttcgtctgg
    24721 cttcttgctg catttgtttt tttaatgtta tttcatcagc taagccagat ttgctgatca
    24781 catctagcaa gtcttggatt tctcgtggcg aactttcttc tgccaccggt aaatattcga
    24841 tttccaccac caaccgcctt aaattttcat cttgcaaaaa gtctaaaaac tctgcaaggt
    24901 tgaattcata atgttgtgag acatatgcat cgaataaaaa ataaacttct gcatatgcat
    24961 catgaataaa ctgaaattct cgttctttta aaatattacg gacagaaact tctttaaatg
    25021 cacgatataa tagaatctgc tctgcctttt ctacttgcgt caaaggtctc tttttccgtg
    25081 cagtttcttg tctcgtaacc tgagtgtgtt gactttcctg aggttcttga cgattttgct
    25141 gatttgtcct tcgaagatcg cgcagttgtt tttgtatcgt ctctctgccg atctgaaact
    25201 ctgttgacag ttgagataga tacatatctt gttcgattgg tgaaacaact cggctaagtt
    25261 caactagtaa ttcttcgagg tattcgatct tgtctttttc attatctaaa tttttccctt
    25321 gttttaaata acgtgattta aaagtaaaga ctgtttcgcg cccatgagcc actaattctt
    25381 ggaaagatgc ttctccatac ttacggagat attcatcagg atcaagcttt tctggaacga
    25441 ctacgacaga aagtgctaat cgactgtttt gccgaagcaa ttcaatccct cgattggttg
    25501 cttcaaaacc tgcgttgtct ccgtcatagc aaaagaccaa ttctttcgtc agcttttcca
    25561 tttgttggat ctgttgagct gttagacttg tccccataga agctaatcca gttttgatac
    25621 cagcctgcca tgcagcaatc acatccataa acccttcgaa caacaaaatt tctccgtttt
    25681 tccggatatc gcttcttgct ttatcaagat taaataaaac ttgtctttta ttgaacagtt
    25741 ctgtttctgg actattcaag tatttgggct gatctttttc ctgccctttt tcaggtgcaa
    25801 gccagcgtcc tgaaaaaccg atcgtctggc cttgagaatt acgaagtgga aacatcactc
    25861 tttgataaaa acgatcagac atacggccat catcatgcgt gacaaacaat ccagagcgtt
    25921 ctaacaattc atttgaaatt gattcattct tgaatacttg ctgaagaaaa gaacgctcat
    25981 taggtgcaaa acctatgttg aattcttcaa ttaattctaa agacaaccca cgactttgca
    26041 aatattctaa agctggctgt ccagcttttg tatgcaatag catgtgatga tagatttccg
    26101 ctgctttttc atgagcagca atcagtttcc ccgtagcaga ttcaggattt tggacagcta
    26161 tagtacgctg acgccattga tcttctaacg gtatctgttc gaagtctgcc acttttacca
    26221 ctgcttctgg aaaacttaga ccttctagtt cttgaagaaa agtaaacacg tttccgcctt
    26281 taccacaacc aaagcaatga aaaatctgct tgtcttctgc aacagaaaaa gacggtgtac
    26341 gttcttcatg aaaaggacac agccccaagt aatttttacc agactttttc aactggacat
    26401 attgtccaat cacctcaacg atattcgttt gactgcgtac tgtttcgata acctcttgag
    26461 gaatccgttg agccattttt tcaccccctg ctctattgat atcttttggg tttaacagaa
    26521 gatatttcag gagaataacc ggtaaaaaat cgcactaact gaaatcagtg cgattttaaa
    26581 ggacacaatt atacatgttt gattgtatca tatttcattg ctttttcaag tataacacat
    26641 ttctatctca ttaagcatca atttgctact cttttcagta actgattttg aatatcagcc
    26701 ttccagtaca atactcgaag aacaatattt acgagcatga atacaaagaa gtatcctacg
    26761 ctctctccca accctaagtt tacttccaaa attccatgga acagcattgc agctacatat
    26821 atccctacga tcgtcgttac tgctaggaag atgcgcaatg ggttgaaggg ataacaagct
    26881 tttacaactg ccatgcaact gatcccaatc agcaagtaat acatcaaagt agtagtttca
    26941 gcatttcccc atgcgaatgt ttttccaagt agatacaccg caatgatatt cactactaca
    27001 agcaaggcat ttggtaaagc attaataagt gctgtaggca aataacggta agtgattttt
    27061 cttttatctt gttcaaaaga taaaaagaag gaaggatacc cttcaatcgc taaatcaatc
    27121 agcgtgattt ggatagggat aaacggaaat ccaattgctg taacggcaca tataatcgat
    27181 aaaataaatg aataaatcgt tttgataaaa aagatgcctg agactttcgt cacattgttg
    27241 accactcggc gtccttcaaa caggacttct ggcaatgtag taaaatcaga gtccaacaat
    27301 accagattcg caatctgacg tgttgctcca tcaccttctg ccatagcaat gctgcaatct
    27361 gcttctctta acgccaaaac atcattcaca ccgtcgcctg tcattgcaac cgttctgccg
    27421 ctttctttca attcatttac cagcaatttc ttttgctgcg gactcactcg tccaaaaacc
    27481 gtatagcgat gagcagcttc tcgcacttcc atttcttcag taatttgaga aagatcgaca
    27541 tacgcatcat aattcggtaa tcctgcacgt ctagcgatat tggagacagt aaaaggattg
    27601 tctccagaaa tcactttcag atccacacct tcctcatgaa gataagctag tgtttcttga
    27661 gcgttttttc ggattggatc atcgatttca aacagagcaa ccggctcaac aaatgctggt
    27721 ttttcttcgt tcaattgctg atttggagcg atccccaaca taagtacgcg atagccattc
    27781 gcttgggctg tttttagact gtccggaagc cgatcttctt ccaccaaacg ttcaggcgct
    27841 cctacgtaca cgactcccag ttctggaaat tccatcgcgc cccatttgcg atcagaagaa
    27901 aatgcgactt tgtttgtcac atcataccga ttagaaagct caaaataatc tctcatagcc
    27961 cgcatagtga tgttgttatc actgctttct gccagatacg tccccataat ttctgggatg
    28021 gctttctcgt acagcggatg tagtgcttct acccgctgga tactcatttt cccttcagta
    28081 atggtccctg ttttatccaa gcataatgta tcgacatggg ccagcgtttc aattgaatac
    28141 atgtcttgaa caaggattcg tttttttgcc agtttggtta cccctgtcgt taatgcaata
    28201 cttattagca ataccaaacc ctttggaagc atgcctaaca aagctgctgc agatgcaata
    28261 acagaaattt tgatagaagc atcgcggatg aacagtgctt ccaaaaatag tatgatcccc
    28321 aaaggaataa tcacgaaact agtgaatttg gatactttgc gaatcgaatc tacaagttca
    28381 gaacgaatag ctttgtgagt ctttgcctcg ctagtcaagc ggacagcata attatctgca
    28441 cctactcgga taacttcagc catgacagaa ccgcttgtca aaaagcttcc agaaagcaat
    28501 tcagctccag tttctttgac aatcaaatca gattcgcctg tcaaaagagc ttcatttgct
    28561 tctgcccgtc cagatataac ctgtaaatcg gctggtatct gttctcctgc tccgattttt
    28621 accaaatctc ctaataccag ctctttagta gaaatcgatt gttctttacc gtcccggatc
    28681 actagtactt ctgattggga aacaatcgat aatttccgta caagatttct tgcatgtatt
    28741 tcttgataaa ttccaataac gatattgacg acaataattg ctaaatacgc catgttggaa
    28801 tacgcaccta caaataacag acaaatccca ataccaaagt tcagaaaatt gaataacgtc
    28861 aatatattat cgctgaatat ttgttttgta ctttttgatg catcttcctg gtagtcattt
    28921 tgctggccag aagccatttt ctgaactacc tcttgctgtg taagtccatt cattttcttt
    28981 ttctccattt tccgccaact ttcaaaaata atcacgtgtt taagtctagc gtgttccaag
    29041 attaaaaaca acttgttttt ataaaattta tagaaaaaca cacgaatgat tttacaaaaa
    29101 agatgccaac ctttttgttt gaatcacccg tgtgctgttt ttattatcaa tctatcttta
    29161 cttatattgt tcctccaaac ggctcatcca ccaacctagt gctgtaccgg ctataaaaag
    29221 aacaggacaa gctagtaccc cagcaactcc caatcccgcg taatcggtag gaatgatcat
    29281 aatcgttgaa gcaaaaacta cacctaaaat gaaatggaat agcttgctat atgcctttga
    29341 gaaaatgtaa tccatgattt ttgacaatcc aagaacacaa agaatcccac caattccgat
    29401 cggcacaatc acttcaagtc gtacttcttt aaatccgtca gccatcgctt tatacaatcc
    29461 catatacacc aaaaaattag aagggctcaa tcctgggacg atcataccca aaccaatcaa
    29521 tccgccggca atcagccatg ttccaaaatt ctgatcgact tgcccgtcaa ataattgagc
    29581 gccgtataat aaaaacaaat aagccagaac aaaagtaaca accatgatga tcacatcaga
    29641 agtatctctt ccttttttcc ctgcttcttt ccataacgca ggaacagttc cagcgataca
    29701 tccgacaaaa aaccataata tcgttgtttc atacgcacct aataaaaaac tgactaaaaa
    29761 agaaagtaga aagatgcctg tcactccgcc tagaccaaca ggcagaaaaa acaaaacatt
    29821 ttctttgaaa ttttttgtaa ggtgggctaa aaaagaaatc agccgctcat acatgccaaa
    29881 gacagctgct aatgcaccac cgctgactcc tggcaagata aatcctgaac cgataaacat
    29941 tcccttcaca aaccgtaaca tccagtcttt ggttgtgtac cccacttctt gattttccat
    30001 tttttgcctt ttttccacta tttgcgtact ccttctttca tttaatttta ttcatgattg
    30061 aacatttcct gctattttac ttaaagactt tttcataggt ctctattatt ttttcattca
    30121 ttttgacaat atcaggataa aacatgttac ctccattatg atagagatag ccgccattgt
    30181 aaagcaagga ctggatccgc caatatcggt attgtttccc ttcctcattt cctaaaagtg
    30241 ggctcagcac atctttagaa tacgcttctg ctaattctac tgtgttttct ccgccatgat
    30301 tttttacata ggcaatataa tctttcccaa aattatatgc ttggacagct gtccataaat
    30361 cgcatccttg tttctttgct tctactatca tttccgccaa atatttgaca ccttgatcaa
    30421 tactgtcagc tggattatca atgatctcag cttctccatg cagactttcc gagctctgca
    30481 tgagatctgt cccttttcct tttgattcgg tcaatattat cgccttgatc aaattctcgt
    30541 agttttccat atgataattt tttgcagctg ttgttatttc tatttgatag ctttccactc
    30601 gttttaccga acgataagtt cttcctcata aaaacaacac cgcaccaata accgtcagaa
    30661 tgatcatgta aaaaagaata cggataatag ataatcgttt tcgcttcatt tcatcaccta
    30721 gttattttga catctttttt tatcttacta tttctttatc ttcttggcta tttcttcctt
    30781 gatttctgtg tacgccttaa gaaaaaatga attttcctgt attttttgac atcttacgaa
    30841 aatgatcgtt gtttttcagt aaaaacaatt gcccaatgac tgatataatc gttatacttt
    30901 acacagtagg tgattatgtg aaaaaagaaa agaaatatgc aaaaatgcca gatgggagcg
    30961 aaatttacta tgaaaagagc ggtcaaggct tccctctctt tttactgcat ggcaatgacg
    31021 gcagcggccg ctttttttca gaacaggtcc ctgtactcga acgctactat accgtgtatc
    31081 tagtagatag ccgaggacat ggtcgttcga caaacgaagc atctatgttg aattttcagt
    31141 taatggccga ggatctgaat acgatcatgt tgctggaaaa aatcgatcag gcagattttc
    31201 ttggatttag tgatggagca aatctagcac tggtatttgc cagcagcttc cctaaaaaag
    31261 ttcatcgatt gattttaaat tctggaaaca cgcttgttaa aggcgttcgt ttttctgcca
    31321 gagtcatcag taatattcat tatgcatggg tatggttact ctctttattt cgcccaagtc
    31381 ttcgcaaaaa tcttttagtt atcaaacttt tgctgcatga catcgggctt acagaaaatg
    31441 atttaaaaaa aatcaactcc cccactttga tcatcgtggg aaaaaaagat gtaatcaagc
    31501 taaaacattc tttgtatata gctaaaacta taccaaaagc ttcctttgtg cttgtgaaag
    31561 aacaaggaca tgaattagct agaaaagatc ctgaacgctt caatcgagaa gttcttcaat
    31621 ttctttctga aacataacta ttaggaatag aaggtgattt atttgttaaa aaataccata
    31681 caatggctga aaaaccacct aggactgttc aaaacaatct ttttgatttc tgtcattgtc
    31741 attattgtcg gtgaattgat gtccatcggt aaaactctat ctatccagca attaggagaa
    31801 acgtttgcca ctatacctgt atggaaaagc ggactaatgc tattgattgg tcttatctct
    31861 gtgttaccta tggttggtta tgatattatt ttgaatcgga tgcttgaaca aaagcagaat
    31921 cctcgttacc tgtttgagac aagctggctg atcaatacga tcaacaatat tgccggtttc
    31981 ggcgggtttg ttagtgtggg gcttcgctct gaactatatg gacaaaagaa agagagcaga
    32041 aacgtgatcc aagcattatc gaaagttttc ttgtttttga tggctggact ttctatttac
    32101 agtctgcttt cttttttact tgtagtcttc acgccagttg ctcctttttt gaagcaatat
    32161 tggatatggt tgatcggcgg cggtctatat tttcctgtcg tctttttatt tactactttc
    32221 aaaaagaaag gattccttgg tggtacctca cttaaaactc aaggacagct attgctagtt
    32281 tcattgttgg aatggaccgg tgtcctgttt agtttccttt ctgttggtta tttaatggaa
    32341 atccggattg atctgtggca aacaattcca ttattcattg ctgcttcagt gataggaatc
    32401 gtgtctatga ttccaggaga aattgggagt ttcgatgtaa tgatgattat cgggttgtcc
    32461 gctattggtg tgccaagaga aatagtcgtt gtgtggatct tgctctatcg cctgttctac
    32521 tacatcattc cgtttttgat tggaatcgtc tttttcttca aaaatatcgg ttccactttt
    32581 gatcagcgtt attctggtat ccctaaacaa ttagcaacag aaattgcgca taaaatcgtg
    32641 gttgtcttat tatacttttc tggaatcatg ctagtacttt ctgccactat cccgcaagct
    32701 tttacagagt ttcgctggct tcatagtttg aacccattga aatttcattt tattatccaa
    32761 tttcctagta tactattggg atttctttta atcattatgg gcagagggat cgcagcacgt
    32821 gtgaaacgag catatttgcc tacaatcttc ttgattgttc tagctttact ttatgtttta
    32881 ttaagtgatt ttagtttcac accggtggtt ttcttgtcta ttctgcttct gatcattctt
    32941 gcttctaaaa acgaattatt tcgggaacaa ttgatctatt catgggagtg gcgtacgatc
    33001 gatggaatca ttattggtgc actgtccttg ctttacatcg ttattggggt atacaatctg
    33061 ccggatttcc ctcatcggcg tcaccatttt atttcctttt tcttatttcc ttctgaaaag
    33121 atttggtttt cgggattgtt ggccatcatc gctgttagtt tcatgatcgt tctatttgtt
    33181 catttccttc aaggagaaaa gaaacagatt ggcgaagcct ttaatgaaga aaaggccctg
    33241 aagatcctta caacttatgg aggaaactcc gatagtcaat tgatcttctt aaaagataag
    33301 cggatgtttg cctatgaaaa agacggtgaa ccaactgttc ttctccaatt cgcttgcttc
    33361 aataataaat gtattgtaat gggtgatcct tctggtaaga aagaagattt tccagaagca
    33421 atcgaagctt ttatcgaaga aacagatcga ctatgctatt taccagtttt ctacgaaaca
    33481 agcgaagaga ttgttatgat tttgcatgaa ttcggctacg atttcattaa gatgggggaa
    33541 gaagcttatg tagacctgaa tagttttaca acttcaggga aaaaaatgaa gggcactcgt
    33601 gctgtcttga atcggatcga acgagaagga tttacatttg atgtcttaca accaccattt
    33661 tctactgagc aaatgagtac tttcaaaaat atctctgata attggttggg ttcacgaaag
    33721 gaaaaagggt tttctttagg ctttttctct gaagattatc tacaaagagc gcctattgca
    33781 gttgtcaaaa atacccaaga agaaattgtc gcttttgcaa cgatcatgcc tacttacacg
    33841 aacaataaag taggaacgat tgatctgatg cgatatgatt ctaaaaaagc accttctggc
    33901 agtatggatt tcttgttctt gcatttgttt gcctatatga aagaagaaca tatccaatgg
    33961 ttcaatttag ggatggcccc tctttcaaat gtcggaactt ccagaaagag ttttttacag
    34021 gaaaggattg catacttggt ttacgagttt ggttctcatt tttattcttt ccatggatta
    34081 agagaatata aaagcaagta cgctacaaac tgggtctcac gttatactct atattctaga
    34141 gatagttgga ttgcatatgt tatgattgct ttgctgatta ttgataatgc gccagttgag
    34201 cagacacaag gtagaataag tggtttgaaa aaatggatac gaagaaagta ttaaaaactg
    34261 cataccgata gatgaacact ctcatgatga ataaaagaga ctgaagtaaa acgaaagttc
    34321 atgcttctac aatctcttct tatacaattg aagaacaaag agcggtccaa acagaaatcc
    34381 tgtttggacc gctcttttat tttaaacatt tttcattacc aaaatattcg agtcgcgttt
    34441 cttatgcttc gatctctgta acgatacctg aacctactgt ccgtccgcct tcacgaattg
    34501 agaatgtcgt tccgttttca acagcgattg gatggatcaa gtcaacatca atcgtcacgt
    34561 tgtcaccagg catgaccatt tctgtatctt ctggcaatgt gattgttcct gtaacatcag
    34621 ttgtgcggaa gtagaactgt ggacgatagt tgttgaagaa tggagtatga cgtccacctt
    34681 cttcttttgt caacacgtac acttctgctt tgaattttgt atgtggagta atggaacctg
    34741 gtttagcaag tacttgtcca cgttcgatat cgtcacgttg gatcccccgt aacaacacgc
    34801 ctacgttatc cccagcttcc ccgtaatcta acgttttacg gaacatttct acacctgtta
    34861 caactgcttt ttgtgtttca ggtttgatcc ctacgatctc tacctcatca ccgacacgaa
    34921 cagcaccacg atcaatacga cctgaggcaa cagttcctcg acctgtaatt gagaagacgt
    34981 cttccactgg caatagtaat ggtttatctg tatcacgttc tggtgttggg atatattcat
    35041 ctactgtatc catcaattcc ataatagcag cttcagcatc tggatcgcct tgcaatgctt
    35101 ttaatgcgga acctttgatc acaggagtgt catcgcctgg gaaaccatat tcgctcaata
    35161 actcgcgaac ttccatttct accaaatcga tcaattcttc atcatcgact aaatcaactt
    35221 tgttcaagaa aacaatcagg tattttacac caacttggcg tgacaataaa atgtgttcac
    35281 gtgtttgagg catcggaccg tcagttgcag aaacaactaa gattgcacca tccatttgtg
    35341 ctgcccctgt aatcatgttt ttcacatagt ccgcgtgtcc tggtgcatca atatgcgcgt
    35401 agtggcgttt ttctgtttca tattctacgt gtgcggtatt gatcgtaatc ccacgttcac
    35461 gttcttctgg agctgcatca atactagcat agtcttgagg atttgctagg ccttttttgc
    35521 ctaatactgt agtgatcgca gcagttaaag tagttttccc atggtcaacg tgtccaatcg
    35581 taccaatgtt aacgtgtggt ttacttctgt cataatgttc ttttgccata atttacaaac
    35641 ctcctaaata atatagatca ctgaaaaatt tcagctctca cctttttatt atagtctttt
    35701 tttgactaaa tcaaaaagaa tacgtccgac ttactttttg taagattcga actaagctta
    35761 ttatacgtca atattggtca aagtcaacta agagcactcg tctgtcttat acgcgaaaaa
    35821 agtacctttt ataaataaaa aaatgtgttt tttgagaaaa acttcaagat agtccttaac
    35881 tttcttttga agcaataaga acaaaatgag gaaaagttta aaattcagct gttgccaaca
    35941 actttcaata gacgaaatct tctatttcgt taataataag gaaagaggtg aagtacatga
    36001 aaaaattaat tggcgtactt gtcgttttag gcatatttct cggcggaagt tttgcagctt
    36061 atcattattt ttacggcggc gaagcttatt atacaaaaat cacgacgaat ggcgaaatgt
    36121 cagctaccca agcagataac ggagagaaat tcacgactta tacctacaag caagcggctt
    36181 ataataaaga tggtgaaaaa aaacaggtga cgcttcgcga agaaagagaa catccattaa
    36241 agatgaatgc gtatcttaaa ctcaaagtca atcctcgtaa aggtgtatta agctgggaag
    36301 aaataaaggc ttctgaagtg cctaaaaatg cggcagaaaa aatcgataat tagtaaatgg
    36361 tcttaaaatt ttattagata aaaagaggct gggacagaag cgtttaactc caagaaataa
    36421 gaaggagttg cttctgcttc cgccgtttat acgtgtttag agcgtgagac aaaagtgttc
    36481 tttacttttg tctcacgctc tttttttgtt atattcgtct tttccgatcc ttctcttgac
    36541 acaattcatt taactgatca ctggtacttg aacacctaat attctagtca tttttataaa
    36601 cgattcaata cttcatccac atttttgatc atcacggata tcgtaccatc cggattattc
    36661 acaaattcaa tcaaatctgg gttgcgatat acatctactg gtacgatcag ttcgatcccg
    36721 tttgatagct tgaatttttg ttttccaaat tttttctcag aaatttcttg tacttctttt
    36781 acaggaggtg cttctgggac aaatccttgc tcttgtactt cttctttaaa agccattttt
    36841 gccgtgatgt tttccttgaa gactttttcc gcaatcatct catgatccaa ccgacctttt
    36901 tcttctatcg tatcatagac agcttctttc aaatcagcca tgatatcaaa ttcttctgtc
    36961 tcaaattttt ttccaagatg cttggctact ttttggatca cttttacatt ttcttctaaa
    37021 gaaggtgcag gaacactttc gatcactctt ccagaaaaat aaagctcttt tttgccagaa
    37081 aactcatatt ttttttcaat taattcgtag gtccaatcag tcaaattcac agcgaatgct
    37141 tcatctgctt tttgtgtttt cgcccctaga attgctcgat tcaaaatcaa gcggttgtcg
    37201 atccctgctt cgtctgcttc aacaaagtga gtgtaagcct cacgataatt gactttcaat
    37261 aaagataagt acatcactgt atctaattca tacaagacaa caaatacatc tgcactaggt
    37321 gcttcttcac tttcttggta gatatcgaac caccgctgaa cgattgcttc acttgcttcc
    37381 acaaaatttt cttgagcgat tttaagtgat tcaacgatca tgctttcttc tgctagttgt
    37441 ccagtttttg tttgagcaga agagattttt tggatcttct tttgaagaaa atcacgaata
    37501 tattcttttg tcagatccag ctctttttgt gaaaagactg gagaaccagt ttctctgtca
    37561 ataatatgca gaattgcttt ttttaaatag atatccattt tctcattccc ttctgtctta
    37621 gtttacggaa aaaaaatcaa aaataccaga atcaaaatca ttttctttta aacaaaaatt
    37681 tcaagagtag aaacaaaatg gattcttttt ttcgaaaaat gcgctacact tatagttgaa
    37741 cgaataaaaa aggagctgaa agtatgaatt cattaacaag cacttatcgt ctatcaaatg
    37801 gttacgaaat cccggtagta ggattcggca catggcaaac acctgatggg gatgtcgctg
    37861 tttcctctgt taaagaagcg ctggctgcag gttatcgcca tatcgatact gcccaaggat
    37921 acaagaatga agaaagcgtc ggccaagcga ttaaggaaag tggaattcct agagaagaga
    37981 tattcttgac aacaaaatta tggaatgcaa accacagcta tgaacttgtg atgtcttctt
    38041 ttgaagaatc attgaagaag ctccaaacag attatttaga tctgtttttg atccactggc
    38101 caaaccctgt tgcttttcgt gataattggg aacaagcaaa tgcagataca tggcgagcat
    38161 ttgaagaact gtatgaagca ggtaaaatca aagcaatcgg tgtgagcaat ttcttacccc
    38221 accatctaga tacactagca aaaacagcaa aaattatgcc gatggtcaat caagttttcc
    38281 ttgcaccagg cgaattacag acaactgtag ttgagtatgc aaaaaaacat gatatgatcc
    38341 tcgaagcata tagtccatta ggcactggta aaatcttcga tgtgcctgaa atgaaacaaa
    38401 tcgctgaagc acatgacaag acgattgcac aagttgcttt gcgttggtcg ctgcaacacg
    38461 aattccttcc tcttcctaag tcagtgacac caagcagaat caaagaaaat actgaattat
    38521 ttgattttga attaacagaa gaagaaatga aacaaatcga tcagttagat ggtgtagtcg
    38581 gcaaagcaaa agacccagat actacccaat tttaagtcga aaggtgattt tatggatcca
    38641 atcgaacgct tgaattctct ctcggaagaa gttacccaga cttttcattc cgattttgtc
    38701 tttttgatcg atgcagagaa aatccaacat tttcctgcaa gaaactggac acatgatcaa
    38761 atcatcgaag aactgaaaaa acggttcgat cattcgctaa tggtcaaacc ctggcacgaa
    38821 catgaagtca tttattcacc tgaactacct gtttttgcct tgataccgaa aaaatagaaa
    38881 gaatggaacg gattatgtat atatacataa tccgttccat tttttatttt tctttcaaaa
    38941 aacgggaaaa tacgcaaatt tttctgttgt aatttgcgta tacgcaagtt tttttgcatt
    39001 actccttttc atttggctgt ttttttctta taatcgaaag ggtatgagcg aaggagagaa
    39061 catatgaaga aaaaacagaa acaaaaatta ttaaatcaat tccgtccttc attggatggt
    39121 gtaagaggac aattattccg ttctctagaa gaagagagct tgcgacgtta tggtttgccg
    39181 ttacaagtca tgcttgatcc caaaaaccgt caaatcttaa cgatcggatt agctgggaaa
    39241 acaaatgaag atacacggat caacgtgcca atcgatgaca acttcaatac tgtattaaag
    39301 agaattcgtt caggagaaaa cgggattttc gaacgattcc gtgacaatct tcttattgaa
    39361 atcgtgtctt actggaatga tcaacgattg aaaaacgatg aaccaactgc gcaagctgtt
    39421 gcgacaccag ttacagaaga aacagtcagt gtgccagaag aaaaaacgga aaaaccaaaa
    39481 gcggacacaa ctgaaaatac acgctcaact gatacgaatg ccgctttaag ttttgctgat
    39541 ttttcaaaag ccgttgctga gtatcctaaa ttttacacag aacaaaatga acatgaagta
    39601 gttattaatg aaaaaaatgc tgatgaacct cgtaaattag caactatttc tatgactact
    39661 gaaaatgagt tcgttatcga aaaagcactc gaacggaaat acaaagtcaa attgactttg
    39721 attcctttga tcgaacaatt tgcagcgaca gcgattactg atcgataaaa attctatttg
    39781 tcataaaagg ctgggattta aaatcccagc ctttttagtc atataataat ttctacagca
    39841 agtaaaaaga aattccttct aacatcaggc tcaaatatca gcttgacctg tccaacaaat
    39901 cacctatgat cctacaatgt taggcgactt ctcttctacc tgaagatatc ttggagttgc
    39961 ctgatttctt aagatgccta accttaacta taaaaaattc taataataaa catattttct
    40021 gatagagaaa aaag
//
LOCUS       DAYYGO010000009        39439 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000009 DAYYGO010000000
VERSION     DAYYGO010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39439
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.009"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..231
                     /locus_tag="ACHA5T_000436"
     CDS             <1..231
                     /locus_tag="ACHA5T_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886041.1"
                     /translation="LLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGF
                     VLLIFLFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            253..1764
                     /locus_tag="ACHA5T_000437"
     CDS             253..1764
                     /locus_tag="ACHA5T_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFM8886042.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            1776..2735
                     /locus_tag="ACHA5T_000438"
     CDS             1776..2735
                     /locus_tag="ACHA5T_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFM8886043.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            2821..3900
                     /locus_tag="ACHA5T_000439"
     CDS             2821..3900
                     /locus_tag="ACHA5T_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8886044.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(4152..5552)
                     /locus_tag="ACHA5T_000440"
     CDS             complement(4152..5552)
                     /locus_tag="ACHA5T_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFM8886045.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            5770..7431
                     /locus_tag="ACHA5T_000441"
     CDS             5770..7431
                     /locus_tag="ACHA5T_000441"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFM8886046.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            7388..8086
                     /locus_tag="ACHA5T_000442"
     CDS             7388..8086
                     /locus_tag="ACHA5T_000442"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFM8886047.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            8102..8623
                     /locus_tag="ACHA5T_000443"
                     /pseudo
     CDS             8102..8623
                     /locus_tag="ACHA5T_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
     gene            9114..10079
                     /locus_tag="ACHA5T_000444"
     CDS             9114..10079
                     /locus_tag="ACHA5T_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6017 domain-containing protein"
                     /protein_id="HFM8886048.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKNLTLKAKGLLSQMLSLPEDWD
                     YTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDAKGKMADMVYTIYEQPVLENP
                     VLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLSKKEKIITDGQSTHSIPIPSP
                     TPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEYAYLIQDRSIDREQLDEIVDL
                     MLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRFVLDCLRENTTKVRNIKQYLR
                     AMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYSCGEGESL"
     gene            10101..10313
                     /locus_tag="ACHA5T_000445"
     CDS             10101..10313
                     /locus_tag="ACHA5T_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFM8886049.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            10388..10864
                     /locus_tag="ACHA5T_000446"
     CDS             10388..10864
                     /locus_tag="ACHA5T_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HFM8886050.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            10861..12669
                     /locus_tag="ACHA5T_000447"
     CDS             10861..12669
                     /locus_tag="ACHA5T_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFM8886051.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            12746..12961
                     /locus_tag="ACHA5T_000448"
     CDS             12746..12961
                     /locus_tag="ACHA5T_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family mobile element protein"
                     /protein_id="HFM8886052.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            13053..13916
                     /locus_tag="ACHA5T_000449"
     CDS             13053..13916
                     /locus_tag="ACHA5T_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB6/TrbL-like conjugal transfer protein,
                     CD1112 family"
                     /protein_id="HFM8886053.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            13953..14489
                     /locus_tag="ACHA5T_000450"
     CDS             13953..14489
                     /locus_tag="ACHA5T_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HFM8886054.1"
                     /translation="MSTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVERL
                     ASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTRGN
                     AEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFARQK
                     TPGWDVWGNEVECDLILT"
     gene            14503..14925
                     /locus_tag="ACHA5T_000451"
     CDS             14503..14925
                     /locus_tag="ACHA5T_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HFM8886055.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            14855..17254
                     /locus_tag="ACHA5T_000452"
     CDS             14855..17254
                     /locus_tag="ACHA5T_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4-like conjugal transfer ATPase, CD1110
                     family"
                     /protein_id="HFM8886056.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            17286..19277
                     /locus_tag="ACHA5T_000453"
     CDS             17286..19277
                     /locus_tag="ACHA5T_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFM8886057.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            19300..19551
                     /locus_tag="ACHA5T_000454"
     CDS             19300..19551
                     /locus_tag="ACHA5T_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HFM8886058.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            19541..20770
                     /locus_tag="ACHA5T_000455"
     CDS             19541..20770
                     /locus_tag="ACHA5T_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1107 family mobile element protein"
                     /protein_id="HFM8886059.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            20767..22848
                     /locus_tag="ACHA5T_000456"
     CDS             20767..22848
                     /locus_tag="ACHA5T_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFM8886060.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            22997..26917
                     /locus_tag="ACHA5T_000457"
     CDS             22997..26917
                     /locus_tag="ACHA5T_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YodL domain-containing protein"
                     /protein_id="HFM8886061.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKID
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLTERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            26918..27862
                     /locus_tag="ACHA5T_000458"
     CDS             26918..27862
                     /locus_tag="ACHA5T_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886062.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            27866..28069
                     /locus_tag="ACHA5T_000459"
     CDS             27866..28069
                     /locus_tag="ACHA5T_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HFM8886063.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMLVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(28369..28605)
                     /locus_tag="ACHA5T_000460"
     CDS             complement(28369..28605)
                     /locus_tag="ACHA5T_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8886064.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(28788..29159)
                     /locus_tag="ACHA5T_000461"
     CDS             complement(28788..29159)
                     /locus_tag="ACHA5T_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HFM8886065.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(29156..30484)
                     /locus_tag="ACHA5T_000462"
     CDS             complement(29156..30484)
                     /locus_tag="ACHA5T_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM8886066.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(30445..30774)
                     /gene="mobC"
                     /locus_tag="ACHA5T_000463"
     CDS             complement(30445..30774)
                     /gene="mobC"
                     /locus_tag="ACHA5T_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFM8886067.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(31032..31403)
                     /locus_tag="ACHA5T_000464"
     CDS             complement(31032..31403)
                     /locus_tag="ACHA5T_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8886068.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            31548..31733
                     /locus_tag="ACHA5T_000465"
     CDS             31548..31733
                     /locus_tag="ACHA5T_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFM8886069.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            31891..32058
                     /locus_tag="ACHA5T_000466"
     CDS             31891..32058
                     /locus_tag="ACHA5T_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886070.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            32676..33335
                     /gene="vanR-B"
                     /locus_tag="ACHA5T_000467"
     CDS             32676..33335
                     /gene="vanR-B"
                     /locus_tag="ACHA5T_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-B"
                     /protein_id="HFM8886071.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            33338..34681
                     /gene="vanS-B"
                     /locus_tag="ACHA5T_000468"
     CDS             33338..34681
                     /gene="vanS-B"
                     /locus_tag="ACHA5T_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-B"
                     /protein_id="HFM8886072.1"
                     /translation="MERKGIFIKVFSYTIIALLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQAQSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            34852..35658
                     /gene="vanY"
                     /locus_tag="ACHA5T_000469"
     CDS             34852..35658
                     /gene="vanY"
                     /locus_tag="ACHA5T_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-B/VanY-G family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HFM8886073.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGRR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            35676..36503
                     /gene="vanW-B"
                     /locus_tag="ACHA5T_000470"
     CDS             35676..36503
                     /gene="vanW-B"
                     /locus_tag="ACHA5T_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HFM8886074.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGEPFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            36500..37471
                     /locus_tag="ACHA5T_000471"
     CDS             36500..37471
                     /locus_tag="ACHA5T_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HFM8886075.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            37464..38492
                     /gene="vanB"
                     /locus_tag="ACHA5T_000472"
     CDS             37464..38492
                     /gene="vanB"
                     /locus_tag="ACHA5T_000472"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HFM8886076.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGTFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            38498..39106
                     /locus_tag="ACHA5T_000473"
     CDS             38498..39106
                     /locus_tag="ACHA5T_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HFM8886077.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(39172..39345)
                     /locus_tag="ACHA5T_000474"
     CDS             complement(39172..39345)
                     /locus_tag="ACHA5T_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886078.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT        10662 a         9471 c        10284 g         9022 t
ORIGIN      
        1 cttttatcct ggatatatgt gatttctgaa aacaaagata tcacattttt acaaattttt
       61 gaacaaagtg catggaaagt attttttgat tttttgagaa atattctttg ttgcgtattg
      121 ttattaggat ttgtactgct catatttctt ttccctcctt ttatttttat ctttgctggt
      181 ggcgtatgga aacttgtatc tattatcttg actataggaa atgaaaggta gggaaagctg
      241 ttggacgaag aagtgaaagt aaaaatcaga gaggatttta cggtaggaga gatcagttct
      301 cggctgtttg gctcatttgt tgagcatatg ggaagtgtcg tctatacagg gatttatgaa
      361 ccgggccatg aaacagctga tggtgacggg tttagacaag atgtcctctc ttatgtaaag
      421 gaattagaat taggtgtcat tcgctatcct ggaggaaact ttacatctgg ttatgattgg
      481 gtggatactg taggtaggaa agaacaacga ccaaaaaaaa ttgatttagc atggcgaggt
      541 atagaaccaa attcttttgg actccacgaa ttttttaaat ggatcagtca tgtacaagcg
      601 aaaccgatat tcacggtgaa tttaggaaca aaaggaattg atgaagcaaa aaatttagta
      661 gaatattgta attttccaaa aggaaccttc tggagtgatt taagaataaa aaatgggcaa
      721 aaaaagcctt ttggcgttaa attatggtgt ttagggaatg agctagacgg tccatggcaa
      781 gtgggggcta agagtgctta tgaatatggt aggttagcaa atgaagcctc aaaagtcatg
      841 aagttagtgg atgattcaat tgaaacaatc ttagttggaa gctctacgcc acgtttacca
      901 tcgtaccctg aatgggatcg tatcgcatta gaagaagctt atgagaatgt agattattta
      961 gctttgcata actatattga taagtatgat gaagaagact tgactaaacc tcctaaaaat
     1021 gagcgtgatg atacagcgac ataccttgca aaagcttatc gattcgatcg tcaaattgaa
     1081 gaaatcgttg ctacttgtga ttatgtaaaa ggatatttga gaagtgagaa aacaatgtat
     1141 ctagcgtttg atgaatggaa tgtacatgcc caaccggaaa aaagacatag ggattttgaa
     1201 gtcggtagtc ccatcgattg gtgttacttt actatggaag atacattatt atttggttca
     1261 ttagggctgg ctattttacg acacgcagat cgcgtaaaga ttagttgtca atcattgtta
     1321 gtaaacacaa tacctttaat actgacagat gctaaaggac aagcatggag gaatccaact
     1381 tattatgtga tgcaacactt atcgaaatat gctaaaggtc aagtattgaa acaacaaatc
     1441 atttgccctg tttatgattg tgaaaagata aaaaatgttc cttatgtgga cagtgtgatc
     1501 gtagataacg gcaatcagct gactattttc ttgatcaatc gcacaaatga acaacaaaag
     1561 ataacgattg aacatgattt caatttgcta gatcaaggag agcactacac attaaccagt
     1621 agcgatctgt tggacaaaaa tacacgtgaa gaaccagaaa aaataaaacc tgaaataaac
     1681 tatttttcaa tagaaaaaca tgacgaatta aatgtaggga tagaaaaata ctcttggaat
     1741 gtaattagaa taaagaaaat gtgaggaaat ttgttatgat gaagacactt tataataatc
     1801 ctatcgttcg gcaacgggca gatccttggg tatataaaca tacagatggt tattattatt
     1861 ttacaggatc ggttccaggc taccaagtga ttgaattaag aagggcaaag tcactgaatg
     1921 aattagaaca tgctgcaagc ctgattgtat ggcaggctca tgaagaagga ccgatgagtg
     1981 aattgatctg ggcacctgaa gtccattata tcaatggaaa atggtatatt tactttgctg
     2041 caagcaatca taaaactata cgtgatgaat ctcatcatca ccgcatgttc gtacttgaaa
     2101 atcagcatat agatccaatg aatacagatt ggatagaaaa gggacaaata aaaacgaaaa
     2161 gggaaacttt cagtttagac ggaacaactt tctcttctca aggaaagcta tattatgtgt
     2221 gggcccaaca agatcctgaa attactggga attctaatct ttatctatcc gaaatggata
     2281 atccttggac gttaaagggg aagcagatcc tcctttctat acctgagtat gaatgggaaa
     2341 aacggggttt ttcagtaaat gaaggtccag cagtccttac tcgcaatggg aaaatattca
     2401 ttacgtattc tggtagtgca acagatgaaa actatgccat gggcctgctg tgggctgatg
     2461 agcacaagaa tcttttatta aaagaatcat ggcataaatt atcggagcca gtattcgttt
     2521 cttctgaaaa aaataagcaa tttggtcctg gtcacaactc gtttacggtt tcagaagacg
     2581 ggtgctttga tattttgatc tatcatgctc gaccagagaa gaaccaaaaa ggggatccgt
     2641 tagacaatcc taatcggcac gcgaacgcgc aatgctttac gtggaatgag caaggatttc
     2701 cagaatttgg tgaacctgtt ccgtatgcct tatgagcaca agaaaccttg atttttgctt
     2761 gaataaacct tgcagactcg ttatgatata tgtacgtaca aataaaaagg ggcaaggcaa
     2821 atggcaaaat atcaagatat tgcagaagag ataaaacaaa agatccttcg tgaggaatac
     2881 gaagtaaata caaccattcc ctcagaattc aagctgcaag aaatatatga cgtcagcaga
     2941 catacgattc ggcaagcaat cgctgtgttg gtaaatgagg gctatctgcg taaagaaaaa
     3001 gggtcgggca catatgtaga tgacagctat aagaaagaaa aaacaccatt gaacagaaca
     3061 aaaacgatcg gtgtcatcac cacatatctc tctgattata tttttccgtc gattatccga
     3121 ggaatcgaaa atgaattacg agaatctggg tattcattgc ttttagccag tacgaataac
     3181 gatcacgaat tggagaaaga gtgtttgaag aaaatgattg cttatggggt agatggtttg
     3241 atcgttgagc ctacgaaaag taatcagtat aacccaaact tagcacttta tgttgctttg
     3301 agagaacaag gcattccgat ggtgatgatc aatgctgctt atgaagagtt aaatgcctct
     3361 tatatttgtt tagatgatgt gaaagtcggt tttatggcag cagaatattt gataaaaaaa
     3421 ggacatcagc gtctgttgtt catcacaaaa atcgacgatc ttcaaggaaa atacaggatg
     3481 aaaggtttta tcagcgcgtg tgaacagtat aatgtgcgtt ttttgacgga agatatcatt
     3541 actttcacca cggaaacacg agaaacaatc gctgaaaaaa cagtacaacg gttgaaagaa
     3601 gaacaagctt tagatggcat catttgctat aacgatcaaa tagctagttt aatgctagat
     3661 aagttgacag aaaatggcta tcatattcca gatgacctat cgatcgtagg aacagacgat
     3721 tcttctttaa gccatttagg gaataaaaaa ttgacaacat tgacgcatcc aaaagaaaag
     3781 ttgggacaag atgccgcacg gtggatcgtc acaacgattg aaaaaggaca ggcacagcca
     3841 aatattctct atgaacctgt actagttgaa cgggaaacag tgaaagaaaa cgaaaaataa
     3901 gagacgggtt tttaccgcct cttattgctc ctacggttac tcgtcgcaat gtttgtgaga
     3961 gcttttgtca tcgctcaagg ctgaacgttt ctttcaccac ctctccacta aacggtgttc
     4021 tcctaccaac tggtcacaag tcacaatatt catcaaacat gagaagctgt tttttgaaat
     4081 tgttccttgt gactggaaac taagcggttc cttcttattt cttgaagctg accacttctg
     4141 tcacaacctc tttaagaatg atggatagaa atacgtttag gataagtttt tttagatgca
     4201 ctttgttcga tttcctctaa agatttccca cgagtttctg gtacgatctc ttgaataaat
     4261 aaaaccccta aaaagcagat gatcccaaaa atgccgaaaa cgatttcttg agacatagaa
     4321 gcagtcatca caggaaaaag caagccaact aggaaagaac caatccaatt aaaagaagag
     4381 gctaggccag atgctcgccc gcggacagct agtgggaata tttcgcctat gattacccat
     4441 gtcagtggag cccatgtgca agaataaaat gcgacataaa tacataaaaa caagagaatc
     4501 aacaacgaat ttgtatgagc atccagtaca gttcctaaaa cagctggtag gatgaatgaa
     4561 agtcccatca cgctaccgcc tattttcaat aaagttcgcc gattgaactt gtctgcaata
     4621 accaaaaaga gcaaagaacc agcaactaaa atgattcctt ggatgatcgg ccacatcaat
     4681 gcatcgctcg ctgcatgccc agtcgctttt tctacgatca gcggaatata gtaaaaaata
     4741 gcattagctc cttgaaattg ctggaatact gctacaccaa taccggcaat gactaaggaa
     4801 cgatattttt ctaaaaacaa agtatgccaa gaagttccgg ttgtcttttc ttgtttgaca
     4861 ttcagctgga tttgttcgaa ttcctgttca gcttcttttg gtgtccgaat atagttcaat
     4921 actctttttg cctcttccaa gcggccagat tgcataagaa aacgcggtga ctctggtaga
     4981 gctaaaacac cgaaaaataa aatcagcgcg gggacagcag cgagccctaa catcacgcgc
     5041 caagccatcg tttcaggcaa cacttttagc aaataatctg caacatagga aaggagcatg
     5101 ccagaagcaa tcatcacttg attgatccct gacaatcttc cccttagtct agcaggagcc
     5161 atttcagaca tataagcagg aactagggca gatgcagcgc caacagctaa acctagaaga
     5221 atccttgctg caatcagaaa ataagaacca ttgtgagggg cagatgcaga aaggacagaa
     5281 ccagccataa aaataagtgc agaaagaaga atcatttttc gtcgtccaag cttatcagaa
     5341 agaagaccag ataaagagcc gccaaggatc gcacctaaca tgaccgaaga cgtgatccat
     5401 cctatcaaag aagctttacc tgccaaaccc caatcatgct gtaaaaaagg taaggcgccg
     5461 gtcataacac caatatcata tccaaataga atgccgccaa atgcgccaaa aaaataaata
     5521 aacgatgagg aaactttttt ttcttttttc ataagtattc tctccttcta gaatttcgag
     5581 aaggcggttg atcagatttt gtaagcaatt tcattttata acttatgtac gtacaaaaca
     5641 agtgtttttt attgtattct tgtaaataac cgcttgacat gtacgtacaa aagtatacaa
     5701 taatcattgt aagcgctact aaaaagacgg tttgatcagg tttgacgaga aggagggagt
     5761 taaatcatta tgaaggaagt aaatgcgcaa agtatgttag atgtcataga tggtcgtaca
     5821 tctttaggga ttgagtttgg atcaactagg atcaaagctg tattgattga ttcatctttt
     5881 actcctattg ccgctggtag ttatgagtgg gaaaacaaac tagaaaatgg tatctggacc
     5941 tattcattag aagatatttg gaaaggactg cgcgtcagtt accgaaaaat ggcagcagaa
     6001 gtattcgaaa tctatggcga gaatcttacg acaatcgggt cgattgggtt cagtgcaatg
     6061 atgcacggtt atctagcatt tgatagcaag gaacagttac tagtaccatt tcgtacttgg
     6121 cgaaatacaa tcactgaaga agcagaacga atattaacgg aaaaatttca atacaatatt
     6181 ccgcagagat ggagtatcgc tcatttgtat caatctattt tgaataaaga agaacatgtg
     6241 aaagaaatta attttttgac aacgcttgca ggttatgtcc attggcagct tactggtgag
     6301 aaagtactag gcgtaggaga tgcatcaggc atgtttccta tcgatccttc aacgaaaacc
     6361 taccatcaag aaatgaaaaa tatttttggt cagttaatcg aaaaagcgca cgtagcgatt
     6421 gacttggatc acattttgcc taaagttcta gtagcaggag aagaagctgg caagttaagc
     6481 gaatcagggg cttatctgat cgaccctacg ggaaatttga gaccaggaat cccggtttgt
     6541 ccgccagaag gggatgcagg aaccggtatg gtcgctacga acagtgtagc caaacggaca
     6601 gggaacattt cggctggtac ttccgccttc gctatgatcg tattagaaaa agaactagaa
     6661 aatgtccatc cggaaatcga catggtcact acaccttcag gagaactagt aggcatggtt
     6721 catacgaata actgctcttc ggatatcaat gcttgggtca gtatttttga agaatttacc
     6781 aattctttgg gtatggagat aagcagagat aaattattca cggtattatt caacaaagcg
     6841 ctagaaggcg atacggattg cgggggatta ctttcttatg gttatttttc cggtgaaaac
     6901 attacaggag taaatgaagg aaggccatta tttgtacgta caccaggtag ccgttttgat
     6961 ttagcgaatt tcatgcggat ccatttagcc agtgcttttg gtgcaatgag aatcggaatg
     7021 gatatattga aatctgagaa tgtccaaatt gatcgtttag tggggcatgg ggggattttt
     7081 aaaacaccag aagttggaca gcggattcta gcatctgcta tggaggcacc agttacagtg
     7141 atggacacag ccggagaagg tggagcgtgg ggaatcgcgt tattagcagc ctatatgatg
     7201 gatcgacatg ggaagtcttt agaaaacttc ttggcagaag acgtgtttgg acaagaccaa
     7261 ggaatcacca tcacaccttc agcagaagaa atagcaggct atcagatatt tatgagacgt
     7321 taccgtgagg ggatcgacat tgaaaaaaca gcgattcatc aattgcaaga aaaaggagtg
     7381 atagaaaatg ctggaacaac tgaaagaaga agtataccaa gcaaatcttg acttgcctaa
     7441 acacggactc gtcaaatata catggggaaa tgtgagcgct tttgatcctg aaacacgata
     7501 tttcgttatc aaacctagcg gagtcagtta tgaagagatg acagcagacg acatggtcgt
     7561 tgtggacttg gacaatcata tcattgaagg taaactcaat ccctcttcgg atacgcctac
     7621 tcatgctgtt ttatatcgtt catttcctca aatcggagga atcgtccata ctcactcgac
     7681 atgggcgacg atctgggcac aagcaggatt ggatgttcct gcgatgggga cgacgcatgc
     7741 agatactttt tatggatcgg taccttgtgc tcgatttttg acacaacagg aaatcgacag
     7801 cgggtacgaa tatgaaacag gaaaagttat catagaaaca ttcaaagaac gcaaaattga
     7861 tcccttagca attccagggg tacttttaca cggacacggt ccatttactt gggggaaaga
     7921 tgcgcaaagt gcggtaatga acgcagtcgt actagatgaa gtatgcaaga tgaatttatt
     7981 taccagacag ctcaattcat tttcagaaga gctgcctcaa cggattttag ataaacatta
     8041 tttacgaaaa cacggaaaaa atgcgtatta cggacaaaac aaataaaagg agtggctaat
     8101 tatgttgaat attggagaaa aagaattttg gtttgttgta ggatcacagc atttatatgg
     8161 ggaagaagca ctgcgagaag taaagaaaca tgcacaagaa atggtcgatg aactgaatga
     8221 aaatggccaa ttgccttatc caatccgcct tcaagaatta gcagttactg ctgatacgat
     8281 cacaaaaatc atgaaagagg tcaattatcg agaagaagta gcaggtgtga tcacttggat
     8341 gcatactttc tcaccagcaa aaatgtggat ccgcgggaca aagcttctac aaaaaccatt
     8401 gcttcattta gctacgcaat ataacgaaag cattccttgg aaaacgatcg atatggattt
     8461 catgaattta aatcaatccg ctcatggtga ccgcgaatat gggttcatca atgctcgatt
     8521 aaataaacaa aataaaatcg tcgttggcta ctggaaacga cctgagatcc aaaaagagat
     8581 tgctgattgg atggatgttg cagtagccta taatgaaagt tttattccaa aaatttaggt
     8641 tatcgctgaa agccccggaa atacgggcat ttcgcaggat atgagaactc aatttactac
     8701 caatttacta ctttcggatt gagccgcata tatgctgaac catactgcta atacccgcca
     8761 ccccaaatac ggcacatcat aaattgaata cgacaccgta aacgcggcgt tcctctttcc
     8821 cgtcatggga gaacaggacg ccgctttttt atgcccaaat agagaaagga ggcgacgcca
     8881 tgctatttac ttccggccct gaccgggcgt ttgaacggct gatgcaggaa aagcccggtt
     8941 tcgatcacta cgacggcggc ggggcggacg cgccggagga ttgcggcacc tgccgatttt
     9001 accgcccgga ttggaaatat caattctgcc agtttgcaga gtgtccctat cagccgggca
     9061 agctgaccga ctatgcagcg gtcaaattca cagtgaaagg agcgaacaac gctatggcag
     9121 ttttccgtgt ggaaaagaac aaaggctata cggtgatgtc aaatcaccac ctgcggaata
     9181 agaacctgac cttaaaggcc aagggcctgc tgtcgcagat gttgtccttg ccggaggatt
     9241 gggactatac cctaaaagga ctgtcctata tcaaccggga aagcatcgac gcgatcagga
     9301 cagcggtatg ggagcttgaa aaagctggat atatccggcg tgagcagggc cgtgacgcaa
     9361 aaggcaaaat ggccgatatg gtctatacca tctacgaaca gccggtattg gaaaacccgg
     9421 tattggaaaa tccaacatcg gataacccgg tgttggaaaa tccgacatcg gataatccgg
     9481 cgtcggaaaa tccaacgcaa ttaaataaag aagtacaaag aactgactta tcaaaaaaag
     9541 aaaaaataat tacagatgga caaagtaccc attccattcc tatcccatcc ccgacccctg
     9601 cccctatgga gggcgaggca gcggctacgc cgccggaacg gaaaaggaca ggaaaggatg
     9661 aggctgtacg gatttatcgg gaaatcattt tggagaacat cgagtatgcc tatctcattc
     9721 aggacaggag cattgaccgt gaacagcttg acgaaatcgt agacctgatg ctggaaaccg
     9781 tctgcaccgc ccgaaagaca atccgtgtcg ccggggacga ctaccctgcc gagctggtga
     9841 aatccaagta catgaagctg accggcgaac atatccgctt tgtccttgac tgtctgcggg
     9901 agaacacaac gaaagtccgc aatatcaaac aatacctgcg ggctatgctg ttcaacgccc
     9961 ccagcaccat ttccaactac tacacggccc ttgtcgcgca cgacatggca cagcccgatt
    10021 ggggaaagcc taaatccggc ctgccggact attcctgcgg cgagggcgaa agcctgtaac
    10081 gagaaaagga ggacgaccct atgaagcagg gcgcattgat ttttgacgag cgaacagacc
    10141 gttacgacat ccgctttgat ctggccgact attacggcgg cctgcattgc ggggagtgtt
    10201 tcgacgtgat gatcggcggc aaatggaggc ccacccgcat tgaaatggcc gaaaactggt
    10261 atcttgtcgg catccgggcc gacgaccttt ccggcctgcg ggtgcggatt tgacctatat
    10321 ctacctgcgg cgtctggtgg ttccgcctta gacgccgctt ttgactttct gacaaggagg
    10381 gatgcgattg caggaagaag ttactcaaaa gaccattgcc ctgtccatga aaacgggcaa
    10441 gctcacggct caagtgctgc aagcggcgct gaaaaagtac ctgcaacacc gggccaaggg
    10501 caaaaacacg ctgcaccacg gccagcagag cctaaagcag cttaaaaagc atggggctga
    10561 tctctccaac attgagatca ccgaggccaa cattggagcg ttcaagccat gcgccaagaa
    10621 atacggcgtg gattttacct tacgaaaaga taagaccacc cagccgcccc actacgttgt
    10681 gattttcaag gccaaggatg cagataacct tgatctggcg tttcgggagt ttaccgccaa
    10741 gacgctttcc aaggagcagc ggccctctat ccgcaaggtt ttgtccgctg tgaagcaaaa
    10801 ggcggcggcc cagaccaagc agcgggccaa ggagaaaatc aaggaaaggg ggctggaact
    10861 gtgaagcctg acgtaaagaa attgctgatc ctgaacctcc cgtatctgat ttttgtgtac
    10921 ctgtttgcga aatgcgggga ggcgtaccgg ctggccgctg gtgctgacct gtcggggaag
    10981 ctcctgcatt ttgcggatgg ttttgcggcg gcttttgcca acccgctgcc aagcctgcac
    11041 ctgtttgacc tgtgtatcgg tatcgccggt gccgtgatcg tgcggctggt ggtgtactgc
    11101 aagagcaaga acgtgaagaa ataccgcaaa ggcatggagt atggcagcgc ccgctggggg
    11161 acggccaaag acatagcgcc ttacatcgac cccaagtttg aaaataatat cctgctcacc
    11221 caaacggaac ggcttaccat gaccggcagg cccaaagacc ccaagacagc gcggaataaa
    11281 aacgtgctgg tgatcggtgg cagcggttca gggaaaacga gatttttcgt gaaacccaac
    11341 ctgatgcaat gctttccgac cacggactat ccgacttcgt ttgtggtgac agatccgaaa
    11401 ggcactttgg ttctggaaac cggcaggatg ttccagcagg cgggctaccg cattaaaatc
    11461 ctgaatacca ttaacttttc caagtccatg aagtacaacc cctttgtcta catccattcg
    11521 gaaaaggacg tgctaaagct ggtaaatacc ctgatttcca acaccaaggg cgagggggaa
    11581 aagtcagcgg aggatttttg ggtgaagtcg gaacggttgt tttattcggc cctgatcggc
    11641 tatatctggt acgaggcccc agcggaggaa atgaacttct caacgctgct ggaaatgata
    11701 aacgccagtg aagcccgcga ggacgacccg gagtttcaaa gccccgtcga tctcatgttt
    11761 gagcgtttgg agcagaaaga ccccgaccat tttgcggtgc gccagtacaa gaaattcctg
    11821 ctgtccgcag gcaagacccg ttcttccatc ctgatttcat gcggtgccag acttgcgccc
    11881 tttgacatcc gggaggtacg cgagctgatg gaggacgacg aaatggagct ggacaccatc
    11941 ggggacgaaa agacggtgct gtatctcatt atgagcgata cggacacgac ctttaatttc
    12001 attctggcta tgctccaaag ccagctaatc aacctgcttt gtgaccgggc tgatgatgtg
    12061 tacggcgggc ggctgcctgt ccatgtacgc ctgattttgg atgaatttgc caacattggc
    12121 caaataccca atttcgataa actcattgcc accattcgaa gccgtgaaat atcggcctct
    12181 attattttgc agagccagtc gcagctaaaa gcgatttaca aggacgccgc cgagatcatt
    12241 tcggataact gcgatagtgt gcttttcctt agtgggcgcg gcaaaaatgc caaggagatt
    12301 tccgatgcgc tggggaaaga aacgattgac agtttcaaca ccagcgagaa ccggggaagc
    12361 caaacgtccc acggattgaa ctatcaaaaa ttaggaaagg agttgatgtc ccaagacgaa
    12421 attgccgtga tggacggcgg caagtgtatc ttgcagctac ggggtgtgcg cccgttttta
    12481 tcggagaaat tcgatattac caagcatccc cggtacaaat accttgccga cgccgacaag
    12541 aagaatacct ttgacgtgga cagatttttg actatcacgc gccggaaacg gcagcaagtg
    12601 gtaacgcagg atgaagtatt cgacctgtat gaaatcgacc tgtcggacga ggacgccgcc
    12661 gctgaataaa cagcggcttt tttcattcca acaacaaaat caaaaaatca ttatgagccg
    12721 gaaaccggca gaaagaggta aatttatggc attttttaat tccgcagtta ctactttgca
    12781 gactatcgtg atcggactgg gcggcgctct gtgcgtatgg ggcggcatca atctgttgga
    12841 gggatacggg caggacaatc cgggggcaaa atcgcagggt gtcaagcagc ttgtcgctgg
    12901 cggcggcgtg gccctgatcg gtgtcactct ggttcccctg ctgtccgggc tgctgggctg
    12961 atcctggcct ttgtccctgt ccaaaccctt aactaatcgc ctccgcgtcc tcccggccag
    13021 accgggaggg cgcgggaaag gaggtgctac ccgtgattag tgaaatcatt gaagaatgga
    13081 taaaaggcat cctcatggac ggtatcacgg gcaacctgtc cgggctgttc gatacggtaa
    13141 atgccaaagt cggagaaatc gccgccgacg tgggacgaac cccgcaggca tggaacagcg
    13201 gtattttcaa tatgctgcac agcctgtcgg aaaccgtgat cgtccccata gccggggcca
    13261 tactcgccct tgtcatgtgc tatgagctta tccagatgat aacggaaaag aacaatatgc
    13321 acgactttga cagctccatg ttcttccgct ggatattcaa atccgcgttt gcaatcctga
    13381 tcgtaacgaa cacatggaac atcgtcatgg gcgtattcga cgccacccaa agcgtcgtca
    13441 atcaaagcgc cggtgtgatt atcgggcaga catccattga ttttaacacc ctgataccag
    13501 accttgaaag ccaattggag gctatgagcg tgggcgctct tttggggctg tggttccaaa
    13561 cccttgttgt gggcctcacc atgaatatcc tttccatctg cattttcctt gtgacctatg
    13621 gccgcatggt ggaaatctat gttgtcacgt cgctggggcc tatccccctt gccacaatgg
    13681 gcaacgcgga atggcgcggc atgggccaag ggtatctgaa atccctgctg gcgctgggct
    13741 tccaagcctt tttaatcatg gttgtggtgg gtatctacgc ggtgctgata cagaatatcg
    13801 gttccgcttc cgacatatcc ggcgcaatct ggggctgtat gggctatacg gtgctgctgt
    13861 gcttctgcct gttcaagacc ggcagtatat caaaggccgt attcggggcg cattgatccc
    13921 aaaatccaag catacacagg aggaacttgc ttatttcaac gtacaacatc atttacgctg
    13981 acccgccgtg gcggtatgac tgcaaaaacg tacagggcgc agcggaaaat cactattcca
    14041 ccatgagcat tgacgagctg tgcgccctgc cggtggaacg gctggcgtcc aaagactgcc
    14101 tgctgttttt gtgggccaca ttccccatgc tgccggaagc tctgcggtta atcaaagcgt
    14161 ggggcttttc attcaagacc gtggcttttg tatggctgaa acagaaccgt aaaagcctta
    14221 catggtttta cgggctgggc cgctggacaa ggggaaacgc ggaaatctgc ctgctggcaa
    14281 cacacggcaa gccgaagcgt cgttctgcct ctgtccatca gtttattatc agcccgattg
    14341 aacaacacag caaaaagccg gatgtgaccc gtgaaaaaat cgtagagctg gcgggcgatt
    14401 tgccccgcgt ggagctgttt gccagacaga aaacccccgg ctgggacgtg tgggggaacg
    14461 aggtggaatg tgaccttatc ctgacgtgaa aggaggtgcc aaatggctta tgtaaccgtc
    14521 cccaaggact taacccatgt gaaaagcaag gttgtgtttg ggcttaccaa acggcaactg
    14581 atctgttttt ccggggcgct ggtgatcggc gtccccttat attttctcac ccgtgactat
    14641 cttaccaaca gcgcggcggc cctgctcatg gtatttgcca tgctgccggg ctttttgctt
    14701 gccatgtacg agcgtcacgg ccagcccctt gaagtggtga ttatgcagat catccagtgc
    14761 tgcttcatcc agccaaagga gcgtccctat cagaccgaca acacctacac cgcccttgtg
    14821 cggcaatacc agatggaaca ggaggtaaag gccattgtcc aaaaaggaaa tcccaagaga
    14881 aaccgaaaaa acaaagctga cccgcgccca gcgaaaagaa attgacgcgg taatccgtaa
    14941 atataagggc gacggcaggc cccacacggc gcagcagagc atcccctacg aggtcatgta
    15001 cccggacggc gtgtgccggg tatcgcccgg cgtcttttcc aagtgcattg agtttgcgga
    15061 catcagctat cagcttgccc agccggacac gcagacggct atctttgaaa aactgtgcga
    15121 cctgtataac tatgtggacg cctccattca tatccagttt tctttcctca atcggaaagt
    15181 tgacccggtg cagtacgcca agagctttga gatcgcgccg cagggggacg acttcgacga
    15241 catccgggcc gagtacaccg gcattctgca aaagcagctt gcaaacggca acaacggcat
    15301 ggtaaagacc aaatacctga cattcaccat cgaggccgaa agcgtgaaag cggcgcgagc
    15361 cagattgaag cgcatcggct ttgacctgtt aggctatttc aagagcatgg gggccgtagc
    15421 ccatgtgatg gacggctggg aacggctgaa cctgctgcat ggcgtgtacc acccggacgg
    15481 cgagatattc aattttgact ggaagtggct ggccccgtcc ggcctctcca ccaaggattt
    15541 tattgccccg tcctccctgt gcttcggcaa tgcaaagaca ttcggcatgg gcggcaaata
    15601 cggcgcggta agtttcctgc aaatcctgtc cccggagctg tcggacgata tgctggccga
    15661 cttcctcaat acggaaagcg gtgttttagt caatctccat gtgcaggcca ttgaacagac
    15721 caaggcgatc aagaccatca agcgcaaaat taccgacctt gacgctatga agattgcgga
    15781 gcagaaaaag gccgtccgca gcggttacga tatggacatt cttcccagcg accttgccac
    15841 ctacggcgag gacgccaaga agctgctgac aaagttgcag acccgcaatg aacggctgtt
    15901 ccagttgacg ttccttgtcc tcaacgtggc cgacaccaag caaaagctga ataacgacgt
    15961 gttccaagcc gcaggcgtgg cccaaaagca taactgtccc cttgtccggc tggactacca
    16021 gcaggaacag ggccttgcaa gcagcctgcc gctgggcgtc aatcagatca agatacagcg
    16081 cagccttacg acttccagcg tggccgtgtt cgtccccttt gtgacgcagg agctttttca
    16141 gggcggcgcg gccatgtatt acggcatcaa cgccaagtcc cgcaacatga ttatgctgga
    16201 caggaagcag gcccgttgcc ccaatgcctt gaagctcggc acccccggca gcggcaaatc
    16261 catgagctgt aaatccgaga tcgtcagtgt gttcttaacg acgcctgacg atatttttat
    16321 ttcagacccg gaggccgaat attaccccct tgtcaaacgg ctccacggac aggtaatccg
    16381 gctgtcgcct accagcaagg actttgtgaa cccgctggac atcaacctga attattcgga
    16441 ggacgataac ccgctggccc tgaaatccga ctttgtgctg tcgttctgtg agcttgtcat
    16501 gggcggcaag aacgggctgg aagccattga aaagaccgtg atagaccggg ctgtgcgcgt
    16561 gatctaccgg ccctatctgg cagacccccg cccggagaat atgccgatcc tgtccgacct
    16621 ccacaaagcg ttgttagatc agcatgtccc ggaggctgac cgggtggcgc aggcccttga
    16681 cctgtatgtg tccggctccc tgaacgtatt taaccaccgt accaacgtgg acatcggcaa
    16741 ccggcttgtg tcctttgaca ttaaggagct gggcaagcag ctcaaaaagc tggggatgct
    16801 cattgtccaa gaccagatat gggggcgcgt cacggccaac cgaagccaag gaaaggccac
    16861 atggtatttt gccgacgagt tccatttgct tttgaaagag gaacagacgg cggcctattc
    16921 tgcggagatt tggaagcggt ttaggaaatg gggcggcatt ccaacaggtg ccacccagaa
    16981 cgtcaaagac ctgctttcct ccccggaaat tgaaaatatc cttgaaaaca gcgacttcat
    17041 tacgctgctc aatcaggcgt ccggggatag aaaaatcctt gcggaacgct tgaatttgtc
    17101 caccgaacag cagaagtaca tcgacaattc cgaacccggc gagggccttt tgatctttga
    17161 aaacgtggtg ctgcccttta ccaaccccat cccgcacaac acccagcttt acaagattat
    17221 gacgacccgc ttaaacgagg tggcgggagt atgacccggc gacgccacaa aggaggtgaa
    17281 ccaccatgaa agagctgaaa gccaaggcga aagtgacgca ggccatgacc cgcgacgggc
    17341 tgacgctgga aaatcaggcc accggcgagg tggagaacat ttccagccgg gaggccgaac
    17401 aggatttgtc cgcgccgggg ccgggcggca ccgccgataa gctgctggaa cgggccgacg
    17461 ccatccatga cggccataag gccaagaaag ccgcgaagca ggccggggct gtggccgccg
    17521 agggggacgc tgccctgcac cggccatccg cccggctcca actgtcggag gaagaacggg
    17581 ccgacccgga tttacagccc tatatccgaa aatcggacaa acgggccgat aagctggata
    17641 aggcccgcgc cgccatcccc aagaagcgcg tcccggtcaa ggaaaccgtc tttgacgagg
    17701 caagcggcaa agcaaagacc gtcctgcact ttgacaaaca ggacaagggg cctccctccc
    17761 tgaaacccaa tccggcaggc cgtcctgttt ccgaggtttt tctattcggc cacgggaaaa
    17821 tccatgaagt cgagcatgaa aacgtgggtg tggagggcgg ccataagggg gaggaacttg
    17881 ccgagcggca ggccggaaaa gccatccgca gcaccatccg gcaccacagg ctgaaaccct
    17941 actgggacgc ggaaaaggcc gagcgcaggc tattgtctgc caacgcggag tatttctacc
    18001 agaaatcttt gcgggataac ccggagatcg cacaggcggc cagcaacccc atttcccggt
    18061 tctggcagaa acagcagatc aagcgccagt acgccaaggc cgcccggaca gcgggccaga
    18121 ccgcaggcgc ggcggcacag ggcgcggcca agacagccgg ggcctccacg aaagcggcca
    18181 aggccgcagc caaagaaagt gaacgggcgg cctcctttgt ggcccgccac tggaaagggg
    18241 cgctggccgt ccttgtgttg gggcttttga tgctgcttat catcggcggc ttacagtcct
    18301 gcaccgccat gttcggcagc gccggaagcg gcatcacggc gtcctcttac ttttccgagg
    18361 atagcgatat gttggccgcc gaggacgcct acgcggagct ggaagccgag ctgcaagact
    18421 atctggacaa ctacgaggcc acccatgact atgacgaata ccattttgac cttgacgaga
    18481 tcagccatga cccctatgtg ctgatttcca ttctgtcggc gctccatgac gggatattta
    18541 ccatcgggga ggtacagggt gatttgtcca tgctctttga caagcaatat atcctgacag
    18601 aaaacgtcgt ggtagaaacc cgttaccgca ccgaaaccga tacatggacg gacgcggagg
    18661 gcaacaccca tacagacacc taccaagtgc cgtatgacta ttacatctgc tacgtcacgc
    18721 tggaaaactt cgacctgtcc catgtgccag tctatatcat ggacgaggaa acgctaagtt
    18781 tctattccct gtatatgtcc acgctaggaa accggcctga tctgttttcc ggcagccagt
    18841 accccaacgc ctccaccctg acgccgccga cctactacga cgtacccccg gaggctttgg
    18901 aggatgccac ctttgccgcc atgctcaaag aggcggaaaa atatatcggt tatccctatg
    18961 tgtggggcgg cagctctccc agcaccagct ttgattgcag cggcttcatt tcgtgggtaa
    19021 tcaatcatag tggatggaac gtgggacggc tgggggcgca gggcctatat aacatctgca
    19081 cccctgtttc cagttcgcag gccaagcccg gcgaccttgt atttttcaag ggaacctacg
    19141 acacccccgg cgtgtcccac gtcggcctgt atgtgggcaa caatgtgatg ctgcattgcg
    19201 ggaacccgat ttcctacacg aaccttactt caaactactg gcaatcccat ttttacagtt
    19261 acgggcgttt accttaacgc cagaaaggag ctttcaatta tggcaaccaa acttgaccgc
    19321 atcgaaaagg acatccagaa aaccaagtct aagatcgcgg agtttcagaa acagcttcgg
    19381 gagctggaaa cgcagaaaac cgaacaggag aatttgcaga tcatccagct tgtgcgcggc
    19441 atgaacatga agccggagga atttgccgcg ttcttacgca gcggcgcaat gcaggccgcc
    19501 cctgccgcaa ccccttacca tgaacaggag gacaatgccc atgaagaata agaccagacg
    19561 gatttcccgt attctccccg cacttctggc cgtgatgctc tgcgtcacgg cctttcccgt
    19621 taccgccctt gcaggcggca atgacccggc accggccccg ctgccggagg ctacaaccga
    19681 acccgctacg ggcggtttgg agcctgaaac agacgaaacc ggcaccgcca cggacgggac
    19741 agaaaccgag gccgaaaaag ataccggccc tctgaccggc aaggacatca ccgacctgtt
    19801 ttccgcgctg ttcggttcca aggtgagcat tgccgccacg gacgacggca tccagatcac
    19861 caccggcacc gacaaaaaag aacctgaaca gaccggcacc gtcaccacca acggcggcag
    19921 gctgaatgtc cgcaccggcg cggggcttga caagaccgcg ttcacccagc ttcccaatgg
    19981 gacgacggtt gaggtagtcg gcacagacgg cgactggatt aaaattctct tgccggagcg
    20041 catcggctat gtccattccg actatatgac cgtcagcgaa aaggaagtag cggctaccgg
    20101 cgagggcggt ttttccctct ccattgaccc ggaggaaatt gcttctctgc tggaactgtt
    20161 caatggcggc ggggccagcg ccgccctgtc cccggacggg aacctgtcac tgattgacga
    20221 catcggcagc gccgcgaaaa atggcaagca gtttatcacc gtggaaacca aaaacggcaa
    20281 tgtgttttat ctgattattg accgggacga cgagggaaaa gaaactgtcc atttcttaaa
    20341 tcaggtggat gaagccgatc tgctgacgct gatggaggac ggggaaaccg tcacccccgc
    20401 tgctgtctgc tcctgtacca cgaaatgcaa ggccggggct gtgaacacga attgcccggt
    20461 atgtaaaacc aacctgaccg agtgcagcgg cccggagccg caggaacccc agccggagga
    20521 acccgaagcc ccggaggaag aacccaaggg cggcgcgggc ggcctgatcg tgttcctgct
    20581 ggtggcgctg gcgggcggcg gtgcggccct ttactatttc aagttcaaaa agcccaaggc
    20641 tgaaaccacc ggcagcgacg atttgggcga gtatgatttt ggggaggacg aggatttgga
    20701 ggacgaggaa cccgaaaccg agtttgaccc ggaagctgat tttgtgagtg aacaggagga
    20761 cgaggaatga catataaact tgtaatcgcg gaaaaaccca gcgtggcccg cagcattgcg
    20821 ggcgtgatcg gggccacgga gaaacacgac ggctatttgg aggggaacgg ctatctggtg
    20881 agctggtgca tcggacatct ggtgtccttt gccgacgccg gacgctatga cgagcgtttc
    20941 aaaaaatggc gctatgagga tttgcccatt ctcccggaaa catggcagta catcgtcccc
    21001 gacgacaaga aacagcagtt tgacgttctc tgttcgctga tgcagcgccc cgacgtgacc
    21061 ggccttgtgt gcgccaccga cgccgggcgc gagggggaat tgattttccg tttcgtctac
    21121 cagatggcgg gatgccaaaa gccctttgag cgcctttgga tttcctctat ggaggacagt
    21181 gctattaagg acggctttgc ccacctgaaa ccgggcggcg actatgatcc gctgtatcaa
    21241 tcggccctgt gccgggccaa ggccgactgg ctgatcggca ttaacgccac ccggcttttt
    21301 tccgtcctgt accacaagaa cctgactgtg ggccgcgtcc agacgccgac cttaaaaatg
    21361 ctggtggaca gggacgccaa gattaccgat ttcaagaaag agaaatatca catcgtccat
    21421 atcacggcgg gcggcgcgga cgcggtaagc agccgctttt ccgacgctgc cgaggcaaac
    21481 accgtcaagg cggcttgtgc aaaagcgcag gccgtttgcg tttccgtgac gcgggagaaa
    21541 aagacggaac agccgcccaa gctctatgac cttaccacct tgcagcggga ggctaaccgg
    21601 cttttcggct ataccgccaa gcagaccctt gactatgccc aggcactgta tctaaagaag
    21661 ctgctgacct atccgagaac ggatagtcaa tatcttaccg acgatatgct gcccgccgtg
    21721 gaaagccttg tgtccggcct gtgggagctg gtgccgtttg ccaagggcct gaacctttcc
    21781 ccgcagtttg accgcatcct gaacagcaaa aaggtgtccg accaccacgc cattatcccc
    21841 accgctgaat ttgtgaagca gggctttggc actctggccg agagtgaaaa aaagctgctg
    21901 tccctgatct gctgcaagct cttatgtgcc gtggccgggc cgcatatcta tgaggccgtc
    21961 accgccacat ttacctgtgc cgggcaggag tttaccgcca agggcaagac cgtcctttcc
    22021 cccggctgga aagagattga acgccggttc cgttcttcct taaagaccga cgccgacgag
    22081 gacgccgagg cggtgcggga gctgccggag ctttgcgagg gccagacctt tgcagacgtg
    22141 gccgcctctg tcaccgagca tttcaccacg ccgcccaagc cctacaccga agatacgctt
    22201 ttgtccgcta tggaacgggc cggggccgag gatatgccgg aggatgccga gcgcaaggga
    22261 ttaggcacac cggccacccg cgccgccatt ctggaaaagc tggtgcagat gggctttgtc
    22321 cagagaaaag gcaagcagct cattcccacc aaggacggca tcaatctggc tgtggtgctg
    22381 ccggaggcgc ttacttctcc ccagcttact gccgaatggg aaaaccggct gaccgagatc
    22441 gccaaggggc aggccgatcc tgacgaattt atggacggca tcgagacgca ggcgcgggag
    22501 ctggtgcaga cctattcctg tatcagtgag gaaaagcaga aattgtttca ggccgagcgt
    22561 gttcctatcg ggacgtgtcc ccgctgtggt gaggctgtct atgagggcaa gaaaaactac
    22621 tactgcggaa accgggcctg ccagttcgtc atgtggaaga atgaccgatt ttttgaggaa
    22681 cgcaagaaag cgttcacccc gaagatcgcc gctgcgctgc tcaaaaccgg caaagtcaag
    22741 gtaaagggcc tctattccgt aaagaccggc aaaacctacg acgggacggt gcttttggct
    22801 gataccgggg gcaagtacgt caactaccgc attgaacaca ggagctaaag gacagtaaca
    22861 cagcaagcat agggagtgtg gctacactcc cggtaaaatc ccccggccgc gtccctgccg
    22921 ggccggggat ttttgcttgt tggggaagtc cccaaccccc tattttttga aacagaacag
    22981 agaaaggagt gaccgcgtga acgaagtatt ttctatccaa gtccataacc ggcagttttt
    23041 ggaacagggc aaacaaggcg tctggctgga tttgcccacc acaaccgaaa cgctgcaagc
    23101 cgccctgcgg gatgttggca ttactgccga caatccgcag gattttttta tcaacgggta
    23161 ttcctgcccg gaggacagac accttgccat tccctatgac atggtgctgg ccgccgacgt
    23221 ggacgagctg aatttccttg ccgcccggct gggccagctt gacgccgccg agatcgcaga
    23281 gctgaacgcc gccctgcaaa atccccgtgg cggctttgag aatatcggcc agatcatcga
    23341 ctacaccgaa aatgtggatt actatgtgca tcttcccgac gttagggggg atggacagtt
    23401 gggcgactac tacctgaacc gttccggcat ggtggatatg ccggaggaat ggaaagccgg
    23461 gattgatacg gcgcagtttg gacgccacat tgcccagcag gaacaaggcg tttttaccca
    23521 gtacggctat ctggtgagaa gcggcgacga atggcagcgc gtccatgagg gccagccggt
    23581 gccggaggaa taccgggtga tgcactaccc ccagccggaa gctgaccggg aggaaccgca
    23641 ggcccagcag ccggaggcat ccgaaagccc cgacaaagcg ccgcccccgg ttatcccgat
    23701 catgctggac ggcatgaaca gcaaagaacg catgaaagtg attactgacc gtctggaaac
    23761 cggcgtacag gagctttttg aaagtagccg ctatatggct tacttatccg ctatgtccaa
    23821 gatgcacgat tacagtctga acaatacgct gctgatcgcc atgcagggcg gttcgattgt
    23881 ggcgggctat aacaggtggc aggatgaatt tgaccgccat gtgaagcggg gcgagaaagg
    23941 cattaagata ctggccccgg ccccgttcaa ggttaagcag cagatggaga aaatcgaccc
    24001 ggccacccag cagcccgtga tcggcgcgga cggcaagccc gtgaccgaag aacaggaagt
    24061 gacggttccg gcctttaagg tggtgacagt ctttgacatc agccagaccg agggcaagga
    24121 ttttcccatg ccctatatca gtgaacttac cggcgacgtg cagcagtatc aggatttctt
    24181 tgccgcgctg gaacggacat caccctatcc cataagcatt gagccgattt cagacggggc
    24241 caagggccgc tgcttctacg caaaacagcg cattgccatc aacgagggca tgagcgaatt
    24301 gcagacccta aagaccgcca tccatgagat tgcccatgcc aagctccacg ccattgaccg
    24361 ggaggccccc gccgaggaac aggcggcccg gccagaccgc agcacccgcg aggtacaggc
    24421 cgaaagcgtg gcctataccg tctgccagta ttacgggctg gacacgtcgg actattcttt
    24481 tggctatgtg gccgattgga gcagcggcag ggagttatcc gagctgaaag cctcccttga
    24541 aaccatccgg gccacggcca gcgaattgat aaccgagatc gacggccatt ttgcccagct
    24601 tcagcaggag cgcgaggccc ggcaggaaca ggaggccgtc acggttgacg ttaccccgga
    24661 ggcagcggcc ccggcgcagg ccgaggaaga acaggccccg gcccttgatc cggcagccga
    24721 gccgcttgtg actgtgctat ggagcgaaag cccccagcta aaggacggcc agcaaatgcc
    24781 gttgcatgag gccgacgcgc tgtttgcccg tctggatgca gcatatccgg cagaggcagg
    24841 ctatgacaaa accgcgttcc gcattgactg tatctttcag ggcgagccgg acagctacga
    24901 ggggcggcag gattttggag atagggacgg ttccctgatc gaacatatcc agagctacca
    24961 ccaattttac gcgcaggacg aaaactggaa aaagcatgta atcgctacaa ccggcataga
    25021 gggctgggag caggacaagg cccatcggga tatgctgtta ggcgagtttg tcccctatat
    25081 gaagctgcat tgcagccttg cccaaatgga acagacggcg cagggccgct tacagtccgg
    25141 ggaaaccctg acgccggagg aaacggccta tttgggggcg cttctctctt atgtgcagga
    25201 gtgccgcccc atgctcaatc agggccagta tcagttgccg gagccgccga aactggctga
    25261 cttcgaccag agcttgcagg actacaaagc acaggtacag gccgagatcg cgcaggaggc
    25321 cgccgcagcc ggtatgaccg tggaggaata cgcggcggct ggctatgaag caccccaaca
    25381 ggacaccttt tccatctacc agcttcgagg cggggaggaa acccgcgact atcggtttga
    25441 gccatacgag cgtttacagg ccgcagggct ggccgtggac agtgccaact atgaccttgt
    25501 ttatacggca ccgctggata aaagcacgtc tttggaggat atttaccgca cattcaacat
    25561 cgaccacccg gcagatttta ggggacacag cctttccgtt tccgacgtgg tggtgctgca
    25621 ccagaatgga caggacaccg cccactatgt agacagcttc ggctatatgg aagtgccgga
    25681 gtttttgcgg gaaaatcccc tgcgtaccgc cgaactgtct acggagcaga acgaaaacat
    25741 gattgacggc attatcaaca atacgccgcc cgaaccgcag ccggaggaac ccgaacagct
    25801 atcttttgag gatgtggcag ttgcttccgg ccagacacag gaggaacccc cggaggctgc
    25861 gcccccgcaa acggctgtcc gctattatcc catcaatgag ggagccgccc gccgcgccaa
    25921 ggaagccatg agcttttctg actaccggcc cggcagcgcc acggcggaat accggcacta
    25981 tgtcgatcag gccgtggaaa ttgcggagcg ccagaaaaag cgtgtggagc cagagtatca
    26041 cgaaaagata gacagcctcc ttgacaccta cgcccgcaag ctggccgaga acatgaataa
    26101 gggctatgag attaccgccc gcgtcccctc tgtcctgatt gccgggccgt ccaatttccc
    26161 ggtacgggcg aaagaaaagc agaacgccgc cagtgaccgc aacatggagg aatttcagta
    26221 cattcagggc ttgcttgaca aaatccgcag caccggcatg ggtggtatca gcgccgacga
    26281 tccgcaggcc gtttccaagc tggaaaagaa actggaaaag ctggaagcgt cgcaggagtt
    26341 gatgaaggcc gtcaatgcct actaccgcaa gcatgggacg ctggacggat gcccccatct
    26401 gaccgaaaga ggcattgaaa acctgaaagc ggatatggcg tcgggctggc actatgagaa
    26461 aaaaccgttc cagtcctggc agctctccaa caataacgcc gagatacgcc ggttaaaggg
    26521 ccgcattgag gaattgaccc gccaaaagga ggcggcctat gtgggatggg agtttgacgg
    26581 cggcactgtg gagatcaacc gggtggccaa ccgcctgcaa atcttctttg aggggaagcc
    26641 ggatgctgct gtacgcgacg agctgaaaag caacggtttc cgctggtcgc ccaaggccgg
    26701 ggcatggcag cggcagttaa acgacaccac aatccgcgtc gctgaccgta tcaagtgcat
    26761 ccagcccctt tccggcgaaa agcccagcgc cttacagctt gcggcccgca gggagaaaga
    26821 aaagccctct atccgggcgc agctcaacgc ggccaagacc gaacaggcca agcagccacc
    26881 ggcgcgggaa aaatcaaagg gattggaggt agagtaaatg cagaagtttg aaaatgtgga
    26941 cgttctggcc gcgctggaac agatcatgcg gcagaacacc gcgttttatc aaagtgactt
    27001 tggcattgac aaagacatta tcaggcggga tgccgccagc gatcaggccg cagacaagac
    27061 cctgctctgg atgtcccggc cctccgggac gtactgtttc cgggaacgtg atgttttcct
    27121 gaaagacacc cgacagtaca acacatggaa attttacggg gagcagaccc gtgacaagat
    27181 actggcctac gcggtggagc tgaccggcac acagggcggc actatccggg gcaatctcta
    27241 tgagctggac tatccgcagc atttccggca cgtcattgaa accgcccagc cggtgagtgt
    27301 gaaccggctc ttttatgagc atggcacccg tgacatcccg gagagccagc gttttgacgg
    27361 aagccctgac cgggtattgg gaaacttttt gtactatgag gcccagcccc atgacccggc
    27421 tgtgctgcag gaggcgctac ggacggagca gcacagccgg gggcgggccg tccccggcga
    27481 tttcaaagtc cacgtcaccg ctttgcatga cagccggatt gaaaccgagg cccggcgtat
    27541 tgtggacagc ctgaaagatc tgtccgcgcc taacagcccc aataaaacgc atttcatggt
    27601 ggagatctcc ccgcactttg cggcgattgc ttccaccaag gataatgacc ggctctttgc
    27661 catgctgccc tataaatccc tgtgttttac cgggatgaaa ggcaggcatg gcgtgtatgc
    27721 cgtgattaac cgggacgagc gccgggatgt gagtattcgc aggccccggc cctctatccg
    27781 caagcagcta tccgacagca aacaggccgc cagcccaaaa aaggcggcgg ccagaaccaa
    27841 aaagaatgaa ttggaggtat aaggaatgat gcactttacc gtggaggaag aaaacctgat
    27901 ctgtatgtac cacaatgcag accggcgcag aacgataaac aggctcaccg ccgtcatgct
    27961 ggttatggat ggggatatgc gggcgcaggc ccagcagacc ctttccaagc tggaacgcat
    28021 gacggatgcc gactatgacg gccagaagtt ccacttcaca gacgaataac gaacagcaga
    28081 cgccgggcgc ggcggccatg tggtcgcttg cgtccggcgt cttttctttt tgcctattcc
    28141 tacgcattta gaacccggta ttgtcgggga taggatgaag tattcgcttg cccctgcaaa
    28201 catgcgggag aagccttgca gcgggcggtt ttcaatccct taaatgcgta ggttgcagct
    28261 ataattagac ccctgaaaca gatatagccg cgcagcgggt gtatctgttt caggggtgtg
    28321 caggtgatag ccgcaaagcg gcgtaagagg gtgcagccct cttgccgtct atctctgttc
    28381 cagcgggaag ctcggcaatg cttccagcag tttcagggaa atgcgctgcc gtatatcttc
    28441 gtcaatgact ttcttctttt tgccgtttgc ctgtctgatc tccaccgtgg aaagctcgtt
    28501 gatgtagtca gcataatggg ccaacacttt ttccgtggcc cacctctcac cggcaacggc
    28561 ggctttgatc gtttcatagt ccagcagctt ttcttgtccg tccatcctga tacccccgtc
    28621 agactgattt tttctatgat accatattcg gaagcatgga accagagaaa acggcgcttt
    28681 tctgctggta aattctacgc atttagaaca gcacttcggc ggggatagga taaaacaccc
    28741 acataccccg ctgaccatgc cggaaaagcc ttgaaatata agggctttta cagctctaag
    28801 tgcgtaggtt tccagtatct tttccgctgg cgctcggcct ctttgcggcg gcgcatccgt
    28861 ttggagcagg ccgcgcagta tttggcccgg ttagacttcg gcgtgaacgt ggccccgcac
    28921 tcacagcagc gtttcacttc gtcccggtct ttggtgatct cggcatacag ggccgcatcc
    28981 agcgggagga cagcggcctt aaaccagcgg cacagaagcg aataggaaat gctctgtacg
    29041 caaatacatt cttcgccgtc gtccagcaac aggcagttgc cgtcacaata gttgcagcac
    29101 tcatgcgtca gcttccgggc cttgcgatat tgagggtaat tcattcgtgg aatactcata
    29161 gctcttgcgt ccattccttt cccggcgtca tacgcaaaat gccgtccaca ttctgcttga
    29221 tgatgccgta ttcctgcatc tgctttttcg cttcgccgta cttctcatac agccgttcct
    29281 tttcctcggc caatcgcttg tactcggctt tcagggcgta cagatcgggg agctttttca
    29341 caccctgtcc ctttagggcc tttgccgcag cttcatacaa aatcaacgtg ctttcatgct
    29401 cacgccggaa ttttgcctta tccgctgcat tgcggtattc catagccacg ggccagagct
    29461 gcttgtaggt agaaatattc tttatcagca ccgccatatc cgcaagccgg tgttccatag
    29521 ctttgagggt agcggcggcc tcgtcgctgg cggcggtgat ctcggccact ttgctttcca
    29581 aatccgggta ggtgtctatc ccatgttcag tcaggaaatt caaagtcttt gcggcctgct
    29641 tcaaattgtg gagcttcgcc cacctctcat agcctgccga ctgctgggcc ttaatgttgt
    29701 tttccagatc aatccgcaaa gaaatcccct tgcgctcttt gggcgctctg gtgataacgc
    29761 ggcctttgat acgctctttg atggcttcct cggtgtaggc ttcccccaat gtcttgcagc
    29821 gggtaaagcg ttcctgtccg ggagcgcgga aagaaataaa ctttcccggc ttgatctcat
    29881 agccttgcgc ctgcatgagc cgcaggaaat catcaaaatc ttttgcctgc gggatagcgg
    29941 cgtcaatggc tgctttgagc ttcgctttcc agctcgtccc tttccgctgg gcgtcccact
    30001 cggcatagct cttgccctta ctctggccgg gcttcaccac ggacaatccg ttttccttgc
    30061 acagcctgtc gctggtgcgc cggatatagg catagctctg tttgttggaa atgtacttgc
    30121 gctgatttac catatccacg gcacagaaaa taatgtgatt gtggacgtgg cccttatcaa
    30181 tatgggtagt cagcacatag ggatatttcc cttgcagcac ttcctcggca agctgcttcc
    30241 cgatctcatg ggcctgttcc ggcgtggctt cgccgggggc aaaggattgt atcaggtggt
    30301 ggcccagatt attgcccttt tggtatgcct gcgccagcag catttcaaat tcaatgtccg
    30361 ctgtttcata agagcaggca aaggaggcca ccagcagctt gccgtcagtt ttgtccgggt
    30421 tctcgatgta gtcaagggct tttttcagcg ttgacttgat aggatgtatc tttgtaactg
    30481 ccatatctgc gccagcgccc cctttatatc ctcgatgtcc tgtgcgtaca ccggccccat
    30541 accgttcagc ctctttgcaa tctgattgac gttgacgccg atcttctgta tctcggcggt
    30601 ctgcgcccgg atgtcgctgg tgtccatgtt gacgatatag ccgtcaatga gcatcttccg
    30661 ggcgtaggct gaaaagttct tcgtatgcag cagcgccatt ttctgttcaa tcatagcccg
    30721 ttcctccggc gtcacaggga cgcgcagcac gatttttcgc tttctgtttg ccatgcttca
    30781 ccgtcctttc cattcagggg tttggggatt tccccaacaa gcaaaacgga cgcggcccgg
    30841 cagggacggg gcaggccgtt tttccgggag tggctacact ccctatgctt gctacgttac
    30901 tgtttctcca ctaacaagac gtttccaaag ccccaaacta cccgcaaaat agccctctgg
    30961 aaagaaaaaa gtgcaaaaaa atagaagcta cgctgtgcag ctcccgctaa aagtaaaaca
    31021 gaggatgcca gttacacatc ctccgtttca ttcttcttcg gcttttcaat tagtatggcg
    31081 ctgtcaatcg cgccctcgat gatcggcagg tattcttcgg ggcagagttt cagcttgtgg
    31141 ctgacccttt gccgcagttc gctttcctcc cgcatgatct ccgggttaaa gtagcgttcc
    31201 acaggaagcc cgcaggcttt aataagctgt atgacaaccg gcagactggg aatggccccg
    31261 tcgttttcga tgttggcaag atagcgccag tcaatattta ttttctctgc cattgctttg
    31321 cgcgaaacgc gctttgcttg ccgggccgct ttcacatccg cgccgaaagt ttcaaagccg
    31381 ggacaatctt caatattcgc catatcgcac ctcccactta cattgtatat ttcatactct
    31441 gcccgtggaa tgttggtata tgcacctttc ttaaagttta tatttcacat tttgtttctt
    31501 ttattcttcg agcaaaagaa ttataatgta ccctgtggag gtgtactatg gactacatga
    31561 cgttgaaaga ggcaggcgag aaatggggtg tgcccccacg atggataaac tactattgtg
    31621 ctgcgggacg catccccggc gctgtaaaga tggcgacgat ctggctcatt cccaaaactg
    31681 cggaaaagcc gattgacagg cgtacaaaga aaggaaaggc ggttactgaa tagtataatg
    31741 aaaccggcgc attttggata tggttttgaa gagagagaaa gcatggtatt ttgtcacgga
    31801 aaaactataa gatgatagat ggcaggctgc tccagaccga taaaaagtat tctcatctga
    31861 aacagaaaga aaagatttac acatggatgt atgaagaagc gaaactttat catgacagaa
    31921 atggaaaatg cccgaaacaa agacgaggaa gctgctgctg tatcagccgt atatggtaga
    31981 attgaaaagg ccggcatctg gattctttat ggtgaagtct ccagacattg gataactgct
    32041 ggtctaatgc gcctttgagt atggggtgat tgattttaat accggggagg tacggctttt
    32101 tgaggaaaat attctgggat ttacatttgg cgattattac atttcctgta actgcaagga
    32161 cacggatgat gaagatatga ggaatataga cttgctgatt ttctactgtc ttgaacggta
    32221 ttaggactga atctgctgcg cggagtgcgg caagagcgac cggagggagt acacacaggc
    32281 cctgcctgtg tgaaaagcca taccgcaagg tgcattcgca aaaaagaggc cgcgcatttt
    32341 gctgaggctt ggggtgtccc caacaagcaa aacgagaacg ccgcaggcgg gcaagtggtg
    32401 gatcagaata tccgtaaagg atattctgat ccattgcttg ccagtattat cgggatgttt
    32461 tgacaaccgc tgtaatcgaa acatcccaaa cattaagaag gtgttaatta ttattatctt
    32521 gatttaattc gtttaaacca ataatataga tacatacacc atgaaaagaa gcgagtatcc
    32581 caatgacgga tgattaaaat ccgaaaagaa atcgctttga aactacaggg aaactacaga
    32641 ctgttatgtt atcttcttaa aatggaggga tttttatgtc gatacgaatt ctacttgtcg
    32701 aggatgatga tcatatctgt aatacagtaa gggcgtttct ggctgaggca ggatatcagg
    32761 tggatgcctg cacagatgga aatgaggcat acaccaagtt ttacgaaaac acttatcaac
    32821 tggttattct tgatattatg ctgcccggta tgaacgggca tgaacttttg cgtgaatttc
    32881 gtgcgaaaaa tgatactccc attctgatga tgacagccct gtcggatgac gaaaaccaaa
    32941 tccgggcgtt tgatgcagag gcagacgact atgtaacaaa gccattcaag atgcagattt
    33001 tactaaagcg ggtggaagcc ctgctgcggc gcagcggtgc gctggcaaag gaaatccgtg
    33061 tcggcaggct gacacttctg ccggaggatt tcacggtact ttgtgacggt acagagctgc
    33121 ccctgacacg aaaagaattt gaaatccttt tgctgctggt gcagaacaaa ggcagaacct
    33181 taacgcatga aatcattttg tcacgtatat ggggatatga ctttgagggt gatggcagca
    33241 cagtccacac tcatatcaaa aatttgcggg ccaagctgcc ggaaaatatc atcaaaacca
    33301 ttcgcggtgt aggataccga ttggaggaat cataataatg gaaagaaaag ggattttcat
    33361 taaggttttt tcctatacga tcattgccct gctactgctt gtcggtgtaa cagcaacact
    33421 gtttgcacag caatttgtgt cttatttcag agtgatggaa ttacagcaaa cagtaaaatc
    33481 ctatcagcca ttggtggaac tgattcagaa tagcgatagg cttgatattc aagaggtggc
    33541 agggctgttt cactacaata accaatcctt tgagttttat attgaagata aagagggaag
    33601 cgtactctat gccacaccaa atgccaatac atcaaatagt tttagacccg actttcttta
    33661 tgtggtacat agagatgata atatttcgat tgttgctcaa agcaaggcag gtgtgggatt
    33721 gctttatcaa gggctgacaa ttcggggaat tgttatgatt gcgataatgg ttgtattcag
    33781 ccttttatgc gcgtatatct ttgcgcggca aatgacaacg ccgatcaaag ccttagcgga
    33841 cagtgcgaat aaaatggcaa acctgaaaga tgtaccgccg ccgctggagc gaaaggatga
    33901 gcttggcgca ctggctcatg atatgcattc catgtatgtc aggctgaaag aaaccatcgc
    33961 aaggctggag gatgaaatcg caagggaaca tgagttggag gaaacacagc gatatttctt
    34021 tgcggcagct tctcatgagc taaaaacgcc catcgcggct acaagcgttc tgttggaggg
    34081 aatgcttgaa aatatcggtg actacaaaga tcattctaag tatctgcgcg aatgcatcaa
    34141 aatgatggat aggcagggca aaatcatttc cgaaatactg gagcttgtca gcctgaatga
    34201 tgggagaatc gtacccatag ctgaaccgtt ggacataggg cgcacggttg ccgagttgct
    34261 gcccgatttt caaaccttgg cagaggcaaa caaccagcgg ttcgtcacag atattccagc
    34321 cgggcaaatt gtcctgtccg atccgaggct gctccaaaag gcactatcca atgtcatatt
    34381 gaatgcagtt cagaacacgc cgcagggagg cgaggtacgg atatggagtg agcctggtgc
    34441 tgaaaaatgc cgcctttttg ttttgaacat gggcgttcac attgatgata ctgcgcttcc
    34501 aaggctgttc accccattct atcgcattga tcaggcgcaa agcagaaaaa gtgggcgaag
    34561 cggtttagga cttgccatcg tacaaaaaac gctggatgcc atgagcctcc agtatgcgct
    34621 ggaaaacacc tcggatggcg ttttgttctg gctggattta ccgctcacat caacattgta
    34681 aatatttaaa acttaaatga ttttgaccga caggtataac cctgccggtc tttttttgct
    34741 gctacaggaa aactacagac tggctacagg gaaagtacag atgcgcttgc cataataaca
    34801 atcgtaccag ccacaaatcg tagttttatt gcaaaggagg cattcgatca aatggaaaaa
    34861 agcaactatc attccaatgc ggatcatcac aaacggcata tgaaacaatc cgtggaaaag
    34921 cgggcttttc tatgtgcgtt cattatttcg ttcacagtct gcacgctgtt tttggggcgg
    34981 agactggctt ccgtattgga ggcaacacag ataccgccca tccctgcaac tcatacaggc
    35041 agcagcactg acgtagtgga gaatttggag gaaaacgctc ttgccaccgc caaagaacag
    35101 ggagatgaac aggaatggag cctgatttta gtgaacaggc agaaccccat ccccgcacag
    35161 tacgatgtgg agcttgagca actatcaaat ggtgagcgga tagatattcg gatttctccc
    35221 tatcttcaag atttgtttga tgccgcaaga actgatggag tttacccgat tgtcgcatcc
    35281 ggataccgaa caacagaaaa acagcaagaa attatggatg aaaaaattgc cgaatataag
    35341 gcgaaaggct acacctctgc acaggctaaa gcggaagcag aaacttgggt ggccgtgccg
    35401 ggaacgagcg agcatcagct tggtcttgct gtggatatca atgcggacgg aattcattca
    35461 acaggcaacg aggtttatag atggctggat gaaaacagct atcgttttgg ttttattcgt
    35521 cgctacccac cagacaagac agagataacc ggtgtgagca acgagccgtg gcattaccga
    35581 tatgttggca tcgaagctgc cacagagatg tacaaccaag gggtttgcct tgaggaatat
    35641 ttaaaaccag aaaaatgaga aaaggatgtt atgttatgga cagaaaaaga ttgacacagc
    35701 gtttcccgtt cctgcttcca atgagacggg cgcagagaaa aatgtgcttt tatgcgggaa
    35761 tgagatttga cggctgtcgc tatgcgcaga cgatagggga aaaatcgctt tcccatttgc
    35821 tctttgaaac ggattgcgca ttatacaacc acaataccgg atttgacatg atataccagg
    35881 aaaacaaggt gttcaactta aagctggcag caaagacctt aaacggctta ttgataagac
    35941 cgggggaacc cttttctttc tggtggctgg tacgccatgc ggacaaagat accccctata
    36001 aagacggcct tacggtgacc aatggtaaac tcaccaccat gtcgggcggc ggtatgtgcc
    36061 agatgagcaa tttactattt tggatgttcc tgcatacgcc attgacaatt atccagcgca
    36121 gaggtcacga agtaaaggag tttccagagc caaacagtga cgagattaaa ggggtggatg
    36181 caaccatttc agagggctgg attgatttaa aagtgcgaaa cgataccgac tgcacctacc
    36241 aaatatgggt gaccttagat gatgagaaaa tcatcggtca ggtgtccgcc gacaaagagc
    36301 cccaagcatt atacaaaatt acaaatggca gtatccagta tgtccgtgaa agtggcggga
    36361 tttatgaata tgcccaggtt aaacggatgc aagttgcctt aggtaccggg gaaataatag
    36421 attgcaagct gctttataca aacaaatgca aaatctgtta tcccctcccg gaaagtgtgg
    36481 atattcagga ggagaaccaa tgagaaaaag tgtgggcatt actgtttttg gatgcgaaca
    36541 ggatgaggca aatgctttcc gcgctttatc gccggatttt catattatcc ctacgctgat
    36601 tagcgatgcg atatcggcag acaacgcaaa attggccgct ggcaatcaat gcgttagcgt
    36661 aggccataag tccgaggttt ccgaggcgac aattcttgcg ctgagaaagg tcggggtaaa
    36721 atacatttct acccgcagca tcggctgcga tcacattgat acgactgccg ccgagagaat
    36781 gggaatctcg gttggcacgg ttgcgtattc gccggacagc gttgcggatt atgctttgat
    36841 gctgatgctg atggccatac ggggtgcaaa acccaccatg cacgccgtgg cgcaacaaga
    36901 tttcagattg gatcgtatcc gggggaaaga actgggggat atgactgtgg gagttattgg
    36961 aaccggccat atcgggcaag cggtcgtcaa aaggctgcgg ggatttggat gccatgtgct
    37021 ggcctatgat aacagccgaa aaatggatgc agattatgtc cagcttgatg agcttctaaa
    37081 aaacagcgat attgttacgc tccatgtgcc gctttgtgcg gatacccgcc atctgatcgg
    37141 tcagaagcaa attggagaga tgaagcaagg cgcattttta atcaacaccg ggcgcggagc
    37201 gcttgtcgat accggggcgc tggtggaggc gttggaaagc ggaaagctgg gtggtgcggc
    37261 actggatgtg ttggagggcg aggatcagtt tgtttatacc gattgctcgc agaaagtgct
    37321 tgaccatccc tttttgtcgc agcttttaag gatgccgaat gtgatcatca caccccatac
    37381 ggcgtactac actgagcgtg tgctgcagga tactacagaa aaaacaatca ggaattgtct
    37441 caattttgaa aggagtttac agcatgaata gaataaaagt cgcaatcatc ttcggcggtt
    37501 gctcggagga acatgatgtg tcggtaaaat ccgcaataga aattgctgcg aacattgata
    37561 cggaaaaatt cgatccgcac tacatcggaa ttacaaaaaa cggtgtatgg aagctatgca
    37621 agaagccatg tacggaatgg gaagccgaca gtctccccgc catactctcc ccggatagga
    37681 aaacgcatgg gctgcttgtc atgaaagaaa gcgaatacga aacacggcgt attgatgtgg
    37741 ctttcccggt tttgcatggc aaatgcgggg aggatggtgc gatacagggg ctgtttgtat
    37801 tgtctggtat cccctatgtg ggctgtgata ttcaaagctc cgcagcttgc atggacaaat
    37861 cactggccta cattcttaca aaaaatgcgg gcatcgccgt tcccgaattt caaatgattg
    37921 ataaaggtga caagccggag gcgggtgcgc ttacctaccc tgtctttgtg aagccggcac
    37981 ggtcaggttc gtcctttggc gtaaccaaag taaacggtac ggaagaactt aacgctgcga
    38041 tagaagcggc aggacaatat gatggaaaaa tcttaattga gcaagcgatt tcgggctgtg
    38101 aggtcgggtg tgcggtcatg gggaacgagg atgatttgat tgtcggcgaa gtggatcaaa
    38161 tccggctgag ccacggtacc ttccgcatcc atcaggaaaa cgagccggaa aaaggctcag
    38221 aaaatgcgat gattacagtt cccgcagaca ttccggtcga ggaacgaaat cgggtgcagg
    38281 aaacggcaaa gaaagtatat cgggtgcttg gatgcagagg gcttgcccgt gttgatcttt
    38341 ttttgcagga ggatggcggc atcgttctaa atgaggtcaa taccctgccc ggttttacat
    38401 cgtacagccg ctacccacgt atgatggccg ccgcaggaat cacgcttcct gcactgattg
    38461 acagcctgat tacattggcg ttaaagaggt gataagtatg gaaaatggtt ttttgttttt
    38521 agatgaaatg ttgcatggcg tccgttggga tgccaagtac gccacatggg ataacttcac
    38581 aggaaaaccg gtggatgggt atgaggtgaa tcgcatcatc ggcacaaagg ccgtggcgtt
    38641 tgctctgcgc gaggcacaaa tccatgcggc tgcgcttggc tatggcttgc ttttatggga
    38701 tggatatcgg ccaagaactg cggtggactg cttcctgcgt tgggcagcgc aaccggagga
    38761 caatctcaca aaagaaaaat tttaccccaa tatagagcga gccgagttga ttacaaaggg
    38821 ttatgtggcc tcacaatcca gccatagccg tggaagcgca attgatctta cgctctacca
    38881 cctggataca ggggaacttg tttcaatggg aagtaacttc gattttatgg acgaacggtc
    38941 gcatcataca gcaaaaggga taggggatgc agaggcacaa aatcgaagat gcttgcgtaa
    39001 aatcatggaa agcagcggat ttcagtctta tcgctttgaa tggtggcact ataagttgat
    39061 tgatgagcca taccccgata cctattttaa ttttgccgtt tcataatgaa agtattggtt
    39121 ttgtatcaga ttttggattt gataaaatct atgctggtca tggacaatct tttactggaa
    39181 agtggttaaa gcatcttttg taatacattg caaattatag tattcaatct gcccagcggc
    39241 agaaaagaaa aaaaccgctg ttgggcagac ctttcccacg cccattttgt attttgctgg
    39301 cggcggtttt gagaaatcaa ggccgccgtt cttttctggc agcaaaaggt gtggcggccc
    39361 ataggaggat gccgctatgc tgttttgcta ttgccatgct ctaatccact tccaccactc
    39421 aaaaaaagcc ttgcccctc
//
LOCUS       DAYYGO010000010        39316 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000010 DAYYGO010000000
VERSION     DAYYGO010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.010"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            120..3398
                     /locus_tag="ACHA5T_000475"
     CDS             120..3398
                     /locus_tag="ACHA5T_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFM8886079.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3939..5846)
                     /locus_tag="ACHA5T_000476"
     CDS             complement(3939..5846)
                     /locus_tag="ACHA5T_000476"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFM8886080.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5976..6851
                     /locus_tag="ACHA5T_000477"
     CDS             5976..6851
                     /locus_tag="ACHA5T_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFM8886081.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6931..7905
                     /locus_tag="ACHA5T_000478"
     CDS             6931..7905
                     /locus_tag="ACHA5T_000478"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFM8886082.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8085..9230
                     /locus_tag="ACHA5T_000479"
     CDS             8085..9230
                     /locus_tag="ACHA5T_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFM8886083.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9367..9711
                     /locus_tag="ACHA5T_000480"
     CDS             9367..9711
                     /locus_tag="ACHA5T_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFM8886084.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            9982..11268
                     /gene="efmA"
                     /locus_tag="ACHA5T_000481"
     CDS             9982..11268
                     /gene="efmA"
                     /locus_tag="ACHA5T_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFM8886085.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11406..12005)
                     /locus_tag="ACHA5T_000482"
     CDS             complement(11406..12005)
                     /locus_tag="ACHA5T_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8886086.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12409..13530
                     /gene="mnmA"
                     /locus_tag="ACHA5T_000483"
     CDS             12409..13530
                     /gene="mnmA"
                     /locus_tag="ACHA5T_000483"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFM8886087.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13776..15197
                     /gene="arcD"
                     /locus_tag="ACHA5T_000484"
     CDS             13776..15197
                     /gene="arcD"
                     /locus_tag="ACHA5T_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFM8886088.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15211..16329
                     /locus_tag="ACHA5T_000485"
     CDS             15211..16329
                     /locus_tag="ACHA5T_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFM8886089.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16389..17681)
                     /locus_tag="ACHA5T_000486"
     CDS             complement(16389..17681)
                     /locus_tag="ACHA5T_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFM8886090.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17831..18391
                     /locus_tag="ACHA5T_000487"
     CDS             17831..18391
                     /locus_tag="ACHA5T_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8886091.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18520..18897
                     /locus_tag="ACHA5T_000488"
     CDS             18520..18897
                     /locus_tag="ACHA5T_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFM8886092.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18830..20902
                     /locus_tag="ACHA5T_000489"
     CDS             18830..20902
                     /locus_tag="ACHA5T_000489"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM8886093.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21113..22060)
                     /locus_tag="ACHA5T_000490"
     CDS             complement(21113..22060)
                     /locus_tag="ACHA5T_000490"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8886094.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            22306..22770
                     /locus_tag="ACHA5T_000491"
     CDS             22306..22770
                     /locus_tag="ACHA5T_000491"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFM8886095.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(22832..23875)
                     /locus_tag="ACHA5T_000492"
     CDS             complement(22832..23875)
                     /locus_tag="ACHA5T_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFM8886096.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(24252..25217)
                     /locus_tag="ACHA5T_000493"
     CDS             complement(24252..25217)
                     /locus_tag="ACHA5T_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFM8886097.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            25451..26296
                     /locus_tag="ACHA5T_000494"
     CDS             25451..26296
                     /locus_tag="ACHA5T_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8886098.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            26487..27674
                     /locus_tag="ACHA5T_000495"
     CDS             26487..27674
                     /locus_tag="ACHA5T_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFM8886099.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            27680..28036
                     /locus_tag="ACHA5T_000496"
     CDS             27680..28036
                     /locus_tag="ACHA5T_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFM8886100.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            28038..28376
                     /locus_tag="ACHA5T_000497"
     CDS             28038..28376
                     /locus_tag="ACHA5T_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFM8886101.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    28452..28708
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            28771..29805
                     /locus_tag="ACHA5T_000498"
     CDS             28771..29805
                     /locus_tag="ACHA5T_000498"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFM8886102.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            29798..30484
                     /locus_tag="ACHA5T_000499"
     CDS             29798..30484
                     /locus_tag="ACHA5T_000499"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFM8886103.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            30508..31356
                     /locus_tag="ACHA5T_000500"
     CDS             30508..31356
                     /locus_tag="ACHA5T_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM8886104.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            31553..32326
                     /gene="sufC"
                     /locus_tag="ACHA5T_000501"
     CDS             31553..32326
                     /gene="sufC"
                     /locus_tag="ACHA5T_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFM8886105.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            32339..33625
                     /gene="sufD"
                     /locus_tag="ACHA5T_000502"
     CDS             32339..33625
                     /gene="sufD"
                     /locus_tag="ACHA5T_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFM8886106.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            33622..34857
                     /locus_tag="ACHA5T_000503"
     CDS             33622..34857
                     /locus_tag="ACHA5T_000503"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFM8886107.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            34844..35314
                     /gene="sufU"
                     /locus_tag="ACHA5T_000504"
     CDS             34844..35314
                     /gene="sufU"
                     /locus_tag="ACHA5T_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFM8886108.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            35319..36710
                     /gene="sufB"
                     /locus_tag="ACHA5T_000505"
     CDS             35319..36710
                     /gene="sufB"
                     /locus_tag="ACHA5T_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFM8886109.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(36805..37947)
                     /locus_tag="ACHA5T_000506"
     CDS             complement(36805..37947)
                     /locus_tag="ACHA5T_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8886110.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(37986..38720)
                     /locus_tag="ACHA5T_000507"
     CDS             complement(37986..38720)
                     /locus_tag="ACHA5T_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8886111.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            38952..39227
                     /locus_tag="ACHA5T_000508"
     CDS             38952..39227
                     /locus_tag="ACHA5T_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8886112.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
BASE COUNT        12758 a         7201 c         8286 g        11071 t
ORIGIN      
        1 gttatatttt ttctttttat aaaatacact tattataatt ttgtttttta ttgacagtaa
       61 aaataaatta ggtaatactt taagtaaacg ctttctaatt tgttacggga ggaatacact
      121 tgttaaaaaa acgtataaaa tacagtgcgc taatgatgaa tctccttatg ctcagtaccc
      181 cggttttaat ttctgtcgtt gaagttgctg ctaacgaaca acagtctgtg aacgaagaaa
      241 atgcacacgt gaacattgaa gggatgtcaa caatggagga aataccaaga actagtgaag
      301 atatggatac ggaagaagga aatgatccta tgatgaaaga atcagaaaat gaaaagacaa
      361 aaaagcaatc tgaagaagca aatgaagagt ggaaaggggt tgttttcgga gaaagcacaa
      421 gtgctagtag gtatgccatt gaaccattag aagacggcgt gcggctctct tccacgaata
      481 acggcggaaa attccagtca agcggatcag atggaatgac ctattattat caagcaatcc
      541 ccaaagacat caactttagg atgcgggcga cgatcgaaat agaatcttgg acctatacga
      601 atgcccaaga aggatttgct ttaatggtaa gagacgcagt accaaaaaat catttgtttg
      661 ggcaggcatt ttattcaaat tcatttgcca tactaggcag tcggatcgaa tatgtctggg
      721 actcggatag acaagaagtg gtcaacacga gcggcagcaa atatacgatg cgacttggat
      781 tggggacacg ggcaatcaca ggaatatcat ctgaagatcc gcaagctgcg ccagctcctg
      841 ggagtgtctc agtggaaaca acacctttag agacttcagc agggttcttg gaaaaagaaa
      901 caggttctta taatatattc ggaaacggtc atgggaatct gtttccggcc acgaactcga
      961 ttactaaact ggacgtagag atgaaaaaaa cgaatactgg tttagaggca tattattacg
     1021 atcctgaaac aggagaagaa atggcttctg acatcatgta tgactgggaa aaattatacg
     1081 cttcagatga atcggttatt tatgcaggat ttgctgcagc aaggaatatg acgatcaagg
     1141 tagaaggaat cgaagttgct tactcagatc ctgcgacaga tcctcctggg caagaaagac
     1201 ctactcgctt gattgatccg atttacactg tgacctctag taatcattca ggaaaccaag
     1261 atcatactat gtcctttaga gcaaatgctg acggattatt gacaattaaa aataaagcaa
     1321 caggagaggt gcttaagaac gcaaaaaact tggctatcac tgcgaatcgg gaatttgatt
     1381 atcagacaaa actgatggaa ggaacgaacg aatttacttt cagttttacg ccagataaaa
     1441 actatccgcc agaagaatac gtagagatga ctagttatga aacaaaagag attcttcata
     1501 cagtggacta ccgtaaacct aaatattcga cagtttatgt aacaccagaa gggtcagatg
     1561 caggaaacgg aagcagacaa aatccattat ctctgacaca agcattgaga tacgcatatg
     1621 ctgggcagac aatcgtcatg gcgcctggaa cttattcctt cgaaagagga ttgacgattc
     1681 caaaaggcgt taacgggaca agagaaaacc cgattcagtt gattgcagag aaaaatccag
     1741 aaaacaagcg tcctgtactt gattttaaag gaactggaaa tgggatgaca ctgttcagtc
     1801 actactgggg tttacgaggg tttgacatta caaatagtgc agctatgcaa aaaggattac
     1861 agatttctgg aaatcataat ttgatcgaag agattcatgc ttatcggaat ggaaatacag
     1921 gaatccaaat cagcggtagc tcaaatgatc ctttcgaagc atggcccgcg cataacttag
     1981 tcaagaactg tacatctttt gaaaatgccg atccaggatt tgaagatgcc gatggatttg
     2041 cggccaaact gacaatagga gaaggaaatg tatttgacgg ttgtatcgcg tatcataatg
     2101 cagatgacgg ttgggacctt tttgctaaaa atgaaacagg ttcgatcgga aaagtaatca
     2161 tcaaaaactc agttgcttat cgaaacggct gggtaccagg aattgaggga gaaggtaacg
     2221 gtaatggatt caaaatgggc ggttcttctt taacgggccc tcatgaattg atcaatagtt
     2281 tgtctttcga aaatcttgcg aaaggaatcg attccaatag cggaacagat attatcgtca
     2341 atagcagtac aagttttaat aacggttcat ataatgtggc tctttatacc tcttcagcag
     2401 gaaacacaga ctaccatgct tctggtatct tatctttccg aacagagaat cttgagatga
     2461 gagagcagat ccgcccattg aatcaaaacg aagatcaaat ctaccactcg ttgaactact
     2521 attggaatga acgagagaaa caatcagaaa atactcttgg acataccata tcaaaagatt
     2581 ggatcgagtc tctaaataca tgttcgaaag aaggtcagca gcctgttacg cgctatgaaa
     2641 atggaagtat ccatgttcat ggactgatgc agattaaacc ggggaatcgt caaacagaaa
     2701 aggaaagagt ggatggtctt ccattatctg taggtgctat ttttgacgga gaaacctcac
     2761 cgtcttcgga atatccagag tatgcaatcg tacaagaacc aaatcagaca aaaccaaatg
     2821 aacaaaataa tccgattatt atacaagaag caaatggtgt ggcggagaat gcagaaggaa
     2881 atatcattga actgaagcct tttacagttt ttgtgtcaac tttcacacca aatcaaggaa
     2941 cgcctgtctt tgaagatatg cgttatttcc cagaaaagat ccaaatcgtt gaaacacagt
     3001 caagtgctca attttctaat cataccgcta ttttttcagc accttcaaaa ggagaatata
     3061 cgctagaagt cacttatcgt tcggaaatat ttgatgggga aaaatggtct gctcatacag
     3121 aatctgttac ccagcaaaaa gcgatcactg tgcgagaaac tgccgatgtg cccccgggaa
     3181 atgacaaacc agaaagagaa aatccaggaa atgaatcacc aaatggaaaa ccaaatggaa
     3241 aaccttctca tccggatagg acagcaaaag aaagtgaaaa aaatggcact caaggactcc
     3301 caaaaacggg agaatttaat aacccgttgc tagcattagc tggaggaatt ttgttaatcg
     3361 gtgtagttgt ttatgtcatg aaacagcgaa aaaaataagt ggaatttctc cttttatgat
     3421 gtaaaacaat tgactgagga aagtaaaaat agaaggaaat gagaccaata gatgtctcac
     3481 ttccttctat ctttttatag atgaggttct ccttgctgct tttgattggc tgttcttatt
     3541 acttgatgca ggacagcttt ttcacaatct ctccactaaa cggtgttcaa tcagctgacc
     3601 ctcggtatta gctcaacaaa cggcaaaata tgaggaacta tttttccgtt tttttctcta
     3661 atactcaggt caggacgctg atttcacaac ctctcgataa acggtgttca aaagccagtt
     3721 tctcggtagt aagtcaaatt tatgcaaaaa tgtggaaagc cattttcaca aaatttttct
     3781 tactacttga aactaagcag ctttttcaca acctctaata catggtattc cagaagtaac
     3841 ttcttcgaaa aataagccga aatgtcacaa aaatttgaga agcgattttc ccaaattcct
     3901 tcttatttct tgaagctgac cacttctggc acaacctctt accagattcg cactcttttt
     3961 tctggcgcta gatacatagc gtcttgttcc gtgataccaa atgtttcata gaattcgtca
     4021 aggttttttg gttgcacgtt tgctcggagt ttagcagggg catggacatc gatttgcagt
     4081 aaaagttgtt ggtattcttt ttttgctttt gtacgccaaa tcgttgccca attgacaaag
     4141 aattcctcga tagaaagatc tgattccgtt tttgctgctt ccaatgcaca gcttagaccg
     4201 cctgcatcag caatattttc cgaaacggtt aattttccgt ttacttttcc gtcagcaaaa
     4261 ggcagaccat cgaattcttc gatcattgct tgtgctttgt tttgaaattg tgtaagatct
     4321 tcttctgtcc accagttgtt caagttgcca tattcgtcaa ataaagcgcc gttattatca
     4381 aatgcatggg aaatctcatg agcgataaca gctccgattc caccataatt agcagagctt
     4441 gattgttcta gactgtaaaa tggtgcttga aggatcgctg ccggaaagac gatcacattc
     4501 cgaaatggat gataataagc attgaccgtg tcagcactca tttcccattc tgaacgatcc
     4561 actggtttgt tccatttgct gaaacgatct tttaatgtga gtttgctgaa aacaagagta
     4621 tttgaaagca aagtaccacc ctgatctgca ggtactgttt tgaactggtc gaataatgct
     4681 gggatcttat cggggtagcc tacttggata cctaagttat ttaattttat gacggctttt
     4741 tcttttgttg catccccaag ccagtcattt tcttctagac gttttttata aacggcgatc
     4801 atttttttca ccatttgttc tacgtctttt ttagcagctt cgccaaaata tttttttccg
     4861 tagtaatcgc caactacttg atcgaattgt ccagaggcaa ggtaatacgc tgcttttttc
     4921 tgaggcattg cttcgtctgt tccagacaat acgcgagaat agattccgct gacttggcgg
     4981 aattcttctg atagataacc actaagagaa cgtactgttt taatgatcat ccagcttttg
     5041 aataacggaa aatgagcttc tgtcaggatg tcattcaaat gatcgaagta cactggatca
     5101 gttacgatca ctttatctgg agttgtccca atcaaacccg tgacaagttt tttcaagtcc
     5161 agtttatctg tgtatttaat aaatgtatca aaagattgcg gattatacat tttgctgtaa
     5221 tctgcgtttt cttctgcact ttttacatga ggagcaatca atttatcaaa ttgcactgct
     5281 tgttccgtga ttgcttctgc ctgacttttt tctttgccgg caagctctaa taactgaaca
     5341 gccatatcaa agaaaacgcc tattagttgt gcaccgttgg gatggtctga tgcataatac
     5401 gtcttatctg gaaggaataa agaaggcgga taagcaaaga aggcgttcgt ttgcgcattc
     5461 ttcatatctg catctacatc cagattaaat ggcaatggta atgtatccaa cagccattct
     5521 ggaaattgct tattcaattc gtcgaaagac tgcaatgatt caactttttc gattataggt
     5581 tgaagcggtt gagcatgcaa ctcatttctt cggtcataat cgtttgccag ttgataatat
     5641 gcaaggaaat gtttcatttt ttctgaagac actttttctg gatgggcaag cagatcgtct
     5701 gtttctccca tcaaaagttg gtcgatcccg tcaactaaat cttggaaacc gcctgtagct
     5761 ggtttatctg cagggatttt tgctgttttt tgccattctt cattgactgc ttcatagaaa
     5821 tcttgtttca atcgttcttt gttcattcat cgatcgctcc ttttttgttt cgtgtgccca
     5881 ttatcttaca aaatgaagga attaacaaat agaacataaa aaattgaaaa ttcgaacaat
     5941 caaactgatt tgtttttttc aatccttcac ttataatgaa aaaaaggagg cgatcccaac
     6001 tgaatcaagt agtggataaa cctttatatt tcaaagtaga taaaaaaatt aaaaaattag
     6061 ccagcactca gcagctgcag tcaagaaaaa gtaaattgct ttttttagct ctggtttttg
     6121 aagatcaaag ttatgtgatt atcgatcaat caggacatcc ggtagaatat tctccagcaa
     6181 aatatactta tcaagagggt ctttcttgta aaaaatggaa gctgatcaat gaagagccaa
     6241 tcgagttatc tagatggatc aacagaaaag aagatattcc cgttttgatc gaagaaaaaa
     6301 ggtcaggaaa ggaactcgca aattgctggg tgggattgcc agaagaacgt tttttgaggt
     6361 ataagaaatg ggctaccccc tcaggctatc tttgtggtac ttacgcggca gcagttctcc
     6421 ttgcctatta tcaagactat cgtaaggggt ggatgcttcc aaatgagatt cgcaagaaaa
     6481 atacggctga ttcgttggtt ttgacgaagg cattgcgtag ccagatccaa ccactaggac
     6541 ttcctacgat tccttttcag gttagtacag ggatttctag ttttttaaag aagaatggca
     6601 atcatgagcg tgcacgagca actcttctag gttcatggca gcgagctaca aaacgtatac
     6661 gtgaaggcaa acctgtgatg attggtattt tgaaagtact tggcagtaca tatggcaatc
     6721 attgggtgac agcgtacgct tattttgaaa ctgaaacagg agaacgttat tacaaagtac
     6781 acgacaactg gggcgattat cataaagtca ttcctgccag ttggagtaac ggaacagttt
     6841 cacttcctta agaatttgct gaaattaaca gaaaatcttg gaaaaatatg ttaaactatc
     6901 gaagaataga gaagttttgg ggaggactac atgagtaata agtatcagga tgacacgttg
     6961 aagattttta gtttgaatgg caaccgtcca ttagctgaaa aaatcgcgaa agtgttcgga
     7021 acagaattag gtaaaagcgt cgtgaagcaa tttagtgatg gcgagatttc catcaatatc
     7081 gaagaaagta ttcgagggga tcatgtgtat atcgttcaat caacgaatgc acctgtcaat
     7141 gattactata tggaactact gatcatgatc gatgcaatga agcgagcaag tgcgaaaacg
     7201 atcaatgtcg tattgcctta ctatgggtat gctaggcaag accgcacggc gaaacctcat
     7261 gaaccgatca ctgcgaaatt gatcgctaac ttgatcgaag aagcaggggc aactcgagta
     7321 ctgactttag atctgcacac ggttcaagta caaggattct ttgatattcc tgtagataat
     7381 ttatttacga tgccgttgtt tgctcattat taccgtcaat taggtctgac gggagatgac
     7441 atcgtagttg tttcgccaaa aaacagcggt gttcagcgtg cacgtagtct ttcagaatat
     7501 ttgaacagca cattagccat cgtagatcat gcagatgatg cttcaactga gggtggttat
     7561 gtgatcggtg atgttcaagg aaagacatgt atcatggtag atgatatctt gaacacagga
     7621 gcaacgcttg cacgagcagc aaatgtatta aaagaaaacg gcgcaaaaga agtatatgcc
     7681 tgtgcgtcac atggattatt gtccgatcat gcgaaaagta ttttagacaa tgcaccaatc
     7741 aaagatattt gtatcactga ttctgttttc acagaagaaa atcgtcatcc agaaaactta
     7801 acaatcgtta catgttcaga attaatgggt gaagcggtca aacgcatcca tgaaaataca
     7861 ccgatgagtc cgttattccg tctggaagaa aaagaattcg aataaacaaa aaaaagaggg
     7921 aaagaagttg tgacagaagt gagcagcttc aaggagctgc gacaagactt ttgtcacagc
     7981 tccttttttc tatgcccatg aaacgtcctc tagacaaaaa tgagctcttc tttggtttgt
     8041 tcgctatttt atgctagaat gtgcctagca ataatttgga agggatgaat gaagtgaaaa
     8101 ctatttatct ggatcatgcg gcaactaccc ccatgcatcc acaagtaatt gaagcgatga
     8161 cggcaatcat gcaagacacg tttggaaatc cttcaagcat acatggattt ggtcgccacg
     8221 cgcatgaaaa attggaaaca gcaagacaaa tcattgcaga cagtctgcaa gcaaaacctc
     8281 acgagatcat ctttaacagt gggggaacag aaggggataa tacagctatc ttagaaacag
     8341 cgttctcgcg tcaaaaagaa ggcagacatc taatcactac agctatcgaa catcctgctg
     8401 ttcttaatac catgaaatat ttagagacaa aggggtttga cgttacatat ctgcctgtag
     8461 atgaaaaagg gaatctgtcg atcgaagagg tcaaaaaagc gttacgagaa gatacgatct
     8521 tggtctctgt gatgtacggg aacaatgaaa tcggaaattt gatgccaatc aaagaaatcg
     8581 gtgaactttt agcggatcat ccagcttatt tccatacaga tgcggtacaa gcgtacggaa
     8641 accaaacgat ccatccagaa gaattaggaa tcgatttatt aagtatctct gctcataaaa
     8701 tcaatgggcc aaaaggcgtc ggtttcttat ataaacgaga cggagtaaat atccctgcat
     8761 tgcttttagg cggggagcag gaagaaaagc ggcgtgcagg tacagaaaat ttagcaggga
     8821 tctggggtat ggcaaaagca gttgaactgc tgtcacctga agaacgtgaa gcacgtaaca
     8881 acaaatataa aggattccag caattgattc ttgatacatt agaagcaaac gaaatccctt
     8941 atcaagtaaa tggtgattta acgaataaac tccctcatgt gttgaatctt cgcttaattg
     9001 gcgaagcaaa tgatttgcta ctgatgaagc ttgatcttaa tggggtcgct atttctactg
     9061 gttcagcttg tacagcagga aatatcgagc cttcacacgt attagaagca atgtatggag
     9121 aagatacacc gatactaaaa gaatccatcc gtatcagctt tggtttggga aataccgaag
     9181 acgacatcca gtatttcctt gatcaattag ttcgtgctgt caaaaaataa ctagtgaagt
     9241 cattcttata tgaacagatg tctgagagaa cgccttcatt ttttagggaa cttaactgaa
     9301 aaacctagta aaaatcagga taaactttta taataaagag aaactaattt tatcgaggtg
     9361 aaaatcatgg catttgaaac aacagcaacg gtactgggcg caccaattag ctatcgtttg
     9421 catcctgatg caaaaagata tacattgcgc gataatggat ttacagaaac aaacggaggc
     9481 aattatcaat tgatccgacc attagatgcc acgccgcaaa gcaaagaagg attcaaatta
     9541 aaaatcactg tatcaaaaga tattcaaaca ttgaaaatgt cgatcactac agcaaatgga
     9601 ttgaaagcag tcaatatttt caaagatcca aaacagaaaa tgagccaaga taaattttat
     9661 tttctgatgg atgggatgat tagccgtggg ttattcgaaa aagtcgaatg atctgtggat
     9721 ataaaaagac aactagtttt gaaacggcct attgttagat gactaacttt gtaaggacga
     9781 cgcaaattag ttgtcttttt tatttttaaa tttaacacat gttgttattt ttaatatttt
     9841 gctgatttat tgtttctaag taaagaaatt taaattgaaa cgattgttat ttagggaaaa
     9901 aggtattttt tgaaaaaaga atcccgtata atgattgttg tgcaaacgct atacaaaatg
     9961 aaaaagaaaa gagggatgtt tatggaaaat gaacagtcgg tcgttttaac gaattggaag
    10021 agaaattatt tgttcttttt atcgggacag tttttgtcag gaatcactag tatggtcgtc
    10081 caatatgcga tcatctggta tttgacaaga gaaaccggtt cggcgacaat tttgagtttt
    10141 gctactttac taggaatgat tcccatggtt ttactaagcc catttgtagg accgctagtg
    10201 gatcgctggg ataaaaaagc tcttttgatt gtcacggata ttattgtagc gattttcgcg
    10261 cttattttag cagtagtcgg aacgatttca gaatccttcc cgatttggct agtttttgtg
    10321 tctttgttta tgcgttcggt cgcacaaaca ttccaaatgc caacgatcca gtcgattatg
    10381 ccgacaatcg ttccatcttc tcacataaca aggacgaatg gacagttagg aatggtccaa
    10441 tcagcgaatt tcatcattgc gccagctcta ggtgcggctt tgttttcagt cgttcctgtc
    10501 aattacctta ttttattaga tgtattgggg gcagtttttg gtgtcggctt attgattttt
    10561 gtgaagatcc caaaagtttc tcctgaaata ttggaagttc cgcttactat ttttaaagat
    10621 gcaaaatttg ggctgcagca attgatggat aataaagggt tatggtacat aacgatcaat
    10681 ggtgcatttg tgatgctgtt gttcatgcca gcaatcagtc tgtatccatt gatgacgcta
    10741 gattactttg gtggttccgt tggacaggcg ggagcagtgg aagtagtcta cgcagtcgga
    10801 atgctcttag gcggtgcact gatcagtttt ataggaacat ggaaagaccg aatgaaacct
    10861 attattatag cctacatcat tatgggcctg acgatcggtg caagcggatt ggttccaaac
    10921 gatagtcaag ggttcttgta ctttcttatc ttgaatgcag gagcaggttg tgcaacgcca
    10981 tatttcaata cactgctgat ggcaatgatc cagcagagtt atgaatcgaa tgttttaggt
    11041 cgtgtcttag ggaacttcaa ctctttgatg aacctggcag gtccaattgg attattattt
    11101 gcaggaccat tagctgatcg tttaggggta gaaaaaatgt tcttgttttc cggaatcggg
    11161 attttattat gcggaatcgt tttattttta acgtcagctg cccgaaatta tgataaagaa
    11221 ttacagaaaa aactggtaaa agaacaccat gagcaaaaag acgaatagta tggaatggag
    11281 ttgtgacaga agtcttgtta caactccatt tctgatccta atgttcctgt ggcaataagc
    11341 ctactcaaat gaaaaatatg agtaattatc tttatttgtt taatacttga tatagggcag
    11401 ttttttcacg acctatcgta cttcctcttt gcttcataga tttctttgat ttgttgctca
    11461 gataacggag tgccaaatgg tagtttttcc tttaagagtt ctctttccgg tgcgtctacg
    11521 cctacattga catcgttctc gataggtacg ctgtaatgat ggatatgtcc atgaagattg
    11581 cggatagtgt cgttaggccc gagcaacatc ggataatgag tcaagtaata ttgttgatgg
    11641 tcgaatttaa cgatggcacc tacatcataa aaataaaatt tgggacttcc gtcagtaagc
    11701 ctaggatcat ttttttctaa ataacggaaa aaagcgcgac tgtcatggtt ccctttgatc
    11761 aagtggattg ttccttgcag ttgagtgacc agttccaaaa tttctgcatt tgctttttca
    11821 tattgcggat gcatagcaag gtcgcctaga tgataaacat ggtcggtttc ttttattact
    11881 gcattccagc tagtgatcaa ctgttggttc atttcatcca catttgcaaa gagccgcgga
    11941 gcaaagtcgt tttttcctaa taagcgttca tggaaaaagt gcatatcact gataaagtaa
    12001 ttcatgtatc ttttgcctcc cttttttctt agagtaaaca aaatcactta tctgagcaac
    12061 tcataacatc taaaaagaaa aggttgtaaa tgagacgtgc agctttaagc gataagaagc
    12121 aattttgaaa aatagttttt cacaatttat aaaattattc cttattgccg aaaggctggc
    12181 cctttttatg agtatgccaa ttttttatat cgaatgctca aaagatgagt gattttttgg
    12241 ttgactatca tgtttgtgta ctgaataggt ccagtagaac agtattttta cgacttgata
    12301 attaagggat gaaattttca gaaatatgac gtataatgta aaatactgaa aagttgcgac
    12361 cttcaactcg tagatgtttc agaatctaat tgaaatgatg gtgatatcat gacagacaac
    12421 agcaaaacac gcgttgtcgt agggatgagc ggcggtgtcg actcatctgt aacagcactt
    12481 ttgttaaagg aacaaggata cgacgtgatc ggaatattca tgaaaaattg ggatgacacg
    12541 gacgaaaacg gtgtatgcac ggccacggaa gattataagg atgtagcgaa agtagctgct
    12601 caaatcggga ttccttatta ctctgtgaat tttgaaaaag aatattggga tcgtgtattt
    12661 gaatatttcc tagccgaata ccgcgcagga cgtacaccta acccagacgt gatgtgcaac
    12721 aaagaaatca aattcaaagc attcttggat tatgcaatgg atcttggcgc cgattatgta
    12781 gctactggac attatgcgca agtcactcgc gatgaaaacg gcgtggtcca catgcttcgt
    12841 ggagtagaca ataataaaga tcaaacttac ttccttagcc aattatcaca agaacagctt
    12901 tcaaaaacga tgttccctct aggcggcatg gaaaaatcag aagtacgggc aattgctgaa
    12961 cgcgcaggcc ttgcgactgc taagaaaaaa gattcaacgg gaatctgctt tattggtgaa
    13021 aagaacttca aacaattttt gagcaactat ctgccagcaa aaaaaggaaa tatggtcact
    13081 cttgatggtg aagtcaaagg acaacatgct ggcttgatgt attatacgat tggtcaacgt
    13141 caaggactag ggatcggtgg cggaggagat tcgcaagaac cctggtttgt tgtaggaaaa
    13201 gatctagcga caaacacatt atatgtgggg caagggttcc atcatcctgc attgtatgcc
    13261 acaagcttgg atgcaagtga aatccacttt acgacgaatg aaccaatgcc aaaagaattc
    13321 aagtgtacag cgaaattccg ttatcgtcag caagacgttc ctgtgacagt tcgcttattg
    13381 gatgataatc gtgcagaagt cgtattcgac gaacctgtac gtgcaatcac tcctggacaa
    13441 gcagttgtat tttatgatgg aatggaatgt ctgggtggtg gtttgatcga ccacgcatat
    13501 caagaaacaa aagttttaca atacgtttaa gcgatattaa atgaatcaaa acagctatgg
    13561 ctaacaaaag aaacaccttt tcttttggat agcatagctg tttttttgca ataaaattaa
    13621 tttttattca ttttatactt gaaatacttg tgtaaaaagt tcataatatc caataatatg
    13681 aataaagttg ctttttgtta agcaagagca gttgcaaaat agaagggaat cttttgcaag
    13741 tgatttgttc aattttataa gtgagggtga tggaaatgga aaagaaatcg acgggtatta
    13801 gcaaatgggg actggttgct ctggttgtca gttcgtcaat cggaagcggt gtgtttggga
    13861 tcacaagtga tctggctagt tctgcagctc cagggccagc gatcctttca tggataatcg
    13921 ttgggattgg tattttagct ttggtattat cgctgaatca tttaggagaa aagcgtccag
    13981 atctagatgg tggtattttc ggttacgcgg aagcttcatt tggtaaatta ggtggcttca
    14041 tcagtggttg gggttactgg ttgtctgcgt ggctgggaaa tgttgctttt gcaacgatgc
    14101 tgatgagcgc catgggagaa tttttcccga tattcaaagg tggacaaaat gttccttcta
    14161 ttttatttgc aagtattttt atttggggtc tgactttatt agtaaacaat ggagttgaag
    14221 gagcgagcgt catcaataca atcgtgacga tctgtaaatt agtgccatta ttcctatttt
    14281 taatatttgt atttatcgca tttaaagtga atatattcac tgctgatttt tggggtaacg
    14341 tatctgataa tctagtgaat ggaacagggc agcaaggaac gatctggttg cagattaaag
    14401 gttgtttgat ggttatgatg tgggtctttg tagggatcga aggcgcaagt gttttagcca
    14461 accgcgcgaa gaaacgttca gaagcccagc aagcatcgat cattggtctg ctttgtttat
    14521 tgtttatcta tatccttgct tcgatcttgc catacggcgt cttatctcaa gaagagttag
    14581 caactatttc acaacctgct atggcaaata ttttaaaagg aatcgtggga aattggggag
    14641 cagcgctgat caatatcggc ttgattattt cgatcattgg ttcttggttg tcttggacga
    14701 tgctcccagc agaaacaacg atgttgatgg ctcgagacgg tctgttgccg aaaggttggg
    14761 gaaaaacgaa taaaaaaaat gccccaactt attcgttagt tgttactgca atcttgacta
    14821 atctattctt attgactttc ttagttactg attatgccta tcaatttgcc tattctttat
    14881 gtacagcggc aattttgatt tgttatctac ttgtcgggat ctatcaagtc cagttttctt
    14941 taaaagaagg aatcaaaaaa caagtagtga tcggtgggat tgctgtagtt tttgaattga
    15001 tgggcatcat tttagcagga ttttcttatg tccttttatg tagtattgcc tatcttcctg
    15061 gtttttgctt ctactggatc gcttgtcgtg aaaccaaaca tcaaattacg ggaaaagaaa
    15121 aagtaatgat tgggctgatt tgtgtggccg cactgctttc aatcgggttg cttgctgtag
    15181 gctggatcaa gatataaggg ggaaagaaac atgaaaatcg caaatttcgg tgtagaagaa
    15241 tggctaaata aatgggaaaa gaaagcggta tatgatattg cacaaagctc gatcgaagca
    15301 ttgacattag acgaactcgt tggcttagat gggactagcg taagtaaatt tttcgaaaaa
    15361 cgcaaaaacg aaccattgga ctatggctgg atcgaaggat cagatgtttt caaacaagaa
    15421 gtcgcttctc tttatcaaac cgtcgatccg gaaaacatct tacaaacgaa tggagcgact
    15481 ggtgctaatc ttttagctct atacgcgtta gtggaagcag gagaccatgt tgtttcgatg
    15541 cttcctactt atcagcaatt atatgacatc cccaaatctt taggtgccgc tgttgacttt
    15601 gttcatttga aggaagaaga agattggcag tttgatttag aacagctaga agccttagtc
    15661 aaacctgaaa caaaaatcat ctgtttgaat agtgcaaaca atcctacagg cacattgcta
    15721 gatcggaaaa ctttagaaaa aatcgcagaa attgctcgta cagtagatgc atatgtattg
    15781 atagatgaag tatatgcgcc tttaacggat aaaggtgaat tcttgtcgat cgtagatgtc
    15841 tatgaaaaag gtatagcaac caattcccta tccaaaacct actccattcc aggtattcgt
    15901 attggctgga cagctactgg acctgagttg gcagaagttt ttcgaaagta tcgagactac
    15961 acgatgattt gcggcggagt cctgtcagat gatcttgctg tacatgcatt gaagaataaa
    16021 gaaaaaatat tggcgcgaaa caaaaagatc attacagaga accttgtgat tttaaaacaa
    16081 tgggtagcca atgaaccgaa agttgaactt gtcgctccca attacgtctc tacttctttc
    16141 attaaattag ccatcgaaga agatgaccag acattttgta tcaatttatt agaagaaaca
    16201 ggagtactat tagtaccagg aagtgccttt gatttaccga agcatgcccg actaggttac
    16261 tgttgtaaaa aagaaacctt ggaaaaggga ttattcctct tatctgagtt cttgaaaaaa
    16321 caggcctaaa caaaaacact gcaaggacag tctgtgacaa ctgttcaagc agtgtttttc
    16381 atttacgctt attgtctatg agaaggagag tgccctgaag ctatggagac tcctgaagct
    16441 ttttcttctg cagtttcttg ctgatggatc tcttgtgtat tgtgtcgtac aagcaagaag
    16501 ttgaccaaaa caagaagaga agtcgaaaga aagacgccgc gataaccaaa tgcactggaa
    16561 acactggagc cgatcatagg ccctactacg ttaccagttg cttggaaaga ttggttataa
    16621 ctgaagatcc gtccagcagc ttgatgaggc gaatattttg tgatcaatgc ttggacagca
    16681 gggagcagac aagcatcaga gataccaatc aaaaaacgta aggcagctaa ttgccaaaca
    16741 ttcgtgacga atgccattgg gatataaacc agaatcgcaa aacccaaacc aatcgcaagt
    16801 attctttcac tcccaatctt gtcacctaag cgaccaaatc ttggagcggc tatcaaagtg
    16861 gcaatcccag gtatcgaagc aatgattccg ctgatcaacg taacattccc atgtccatgc
    16921 agcaattggc gaatgtacag actgatgatt ggactaatgg aattgttgga tgcttggatg
    16981 atcatcgttg tgacgaacat accgatgacg acgtgtggat atttcaagtc tttgaaaatc
    17041 tgtttggcag aaaccatgtt ttcttttttc actgggacaa atttttcatg gacgaaaaac
    17101 agactcaaca agaagacgaa aaataagatg atccctgtaa tgaaaaaggt agggcgatag
    17161 ccaaacgctg aagcagagat cccgccaaga agcggtccaa gtaatgttcc tgtaacagaa
    17221 ccagttgcta aggttccaag aacttgacca cttttttctt ttggcgtccc agttgcgaca
    17281 agggcagtgg cattactgat ataaccagaa aaaatccctt gaagcagacg caatgcgact
    17341 aattgataca cactagtcac gcaacccatc aaagaaatga cgattgccat tcctaatgaa
    17401 gcacgcaaca gcatcaattt tcttcctttt tggtcggcta attttcccca ccaaggagag
    17461 ataagcgtag tcaccaagaa agtagatgaa aaagtcaatc cgctccaaaa gttcaattgt
    17521 gacgtactat aattacctaa cgtatctatg taaagtgaca taaaaggcat gactaaacta
    17581 aatccgatac cagccataaa tgttccaaac cataagacaa acaagttttg ttcccaagtt
    17641 tctcttttcc taaataattt ttcctttatt cttgcagcca attagactcc ttctttctga
    17701 aaattttcat ctacagctct ttattataaa ctacaatatg tagtttatca atgaaagtct
    17761 cttttttctc tttctttttc gtggtaaact gaaagagtga aggatttatt ttatgagagg
    17821 aaacatggaa atgaaaaaaa gaaatctgtc tcaagagaaa atcatcgact gctttcgaga
    17881 actagcagaa gagatggatg ttcagcaaat aacctttcag catttagcca aacgcttgga
    17941 tatcaaggct ccctcgctgt ataaccattt caaaaatatt cgtgatgtca aaacagcgct
    18001 aacagcgaaa cttctgcagg aacttaatga ccagctgcga cgggctcttg tgggaaaaag
    18061 tggtcaggaa gcactgcaga tctatgcaga aacttatcga tcctttgctt tttcaaatca
    18121 agcagtgtat gagttactga tcagtgtacc gcataccaat gaagctgttt tattagatgg
    18181 aatccatgaa acgaatcaga tcattttgca gatatttgag gcttttcaat taacgaaaga
    18241 agaaaaagtt catcgaagca gggaacttcg tagtatgatc cacggttatc tttccttacg
    18301 ttttttagga tactttacaa aagaaccaac aatcgatcca gaagacagct atttttggat
    18361 gatcaataac tttatcgcta ctttgcctta aacaatcaga ttcatttgta acaattttaa
    18421 gatgttcatt aaatgaccct tttataagag aaaattcttg caactttctt gaaaagtgac
    18481 taagatgagt ctagaaaagc tgaaagaagg agcacgagta tgtatcaagt aattaccatg
    18541 ttcggggaca atgagccttg gtggtttttt gaagactggg aagaagatat tgaagaagaa
    18601 gaaacattcg agtctttcga agaagcacga caagcgtacg aaaaacaatg gaacgaaatc
    18661 catcaaaatt atgaatatat caatgcaaaa agtaactttc tcagcgcctt ttggaatgac
    18721 ggagatgagc gctggtgtga agagtgtgac gatgatttac agcaatacaa gggacttgcg
    18781 ttgttgaaaa atcatcagcc catcacagta gaaagcagaa aggaatttta tgaaacaact
    18841 aattctagcg gaaaaaccaa gcgttgcgaa agacctaagc aaggtgcttg gtgctaatca
    18901 aaagcacaaa aattattatg aaggacccaa ggtgatcgtt acttgggccc ttggtcattt
    18961 attaggattg aaaatgccgg aagatctgaa taaggaatgg cagagctggc aaatggacac
    19021 gttgccaatg atccctaaaa aactaggaat caaaccactt cctaaaacag gacatcaatt
    19081 aaaagcaatc aagcagctgg ctcagcgaaa agatgtctca gaagcagtca ttgcgacaga
    19141 tgccggaaga gaaggggaac ttgttgctcg ctggatctta gagtatgtgc atttcaataa
    19201 accagtgaag cggttatgga tctcttctca aacagataaa gcaatcaaaa ctggattcaa
    19261 gcaactaaga cctgcgaaag aatatgatgc attgtatgaa tctgctcttg cccgggcaaa
    19321 agcggactgg ctggtaggat tgaatgtgac cagagcctta actgtgaagt acgcggataa
    19381 tttatcagca ggccgtgtgc agacaccgac actagcatta gtgagagacc aagaaaagaa
    19441 aatcgagcag ttccgtccac aaacgtattt cacgatcacg ttgcaagctg gaaaagaaca
    19501 agccaaaatg cttcaaaaaa atcaatttgc tttaaaaaca caagaagaag cacaaaaact
    19561 tgttcaattg atcagtacaa gcaaaggaac tgtcacagaa gtccaagaaa aattaaaaac
    19621 ggaatctgcg ccattgcctt atgatttaac agagatccaa agagaagcca atcaacgcta
    19681 tggctattct gcaaagaaaa cattaagtct tgtacaaagt ttgtatgaga cccataagat
    19741 cgttacttat ccgcgtacag acagcaaata tctgacaaat gacatgaaaa gtacgatgaa
    19801 agaacgattg caagcagtca gcgactttgc tcctgaggtc aagcagtatt tgaaaaacgg
    19861 agccatcgtc caacagacaa aagtattcca agacagtaaa gtgaccgatc accatgcact
    19921 tttgccaact gaaaacagag cacgttatga aaaattaagt aatgaagagc gaaaaatcta
    19981 tcaaatgatt gtctctcgtt ttttaggact gtttgctcaa ccccataaag tcagccaaac
    20041 aaaagttacg gttgaatttg gtaaagaacg attcattttt cggcaaaatc gtgtcgtcca
    20101 agcaggatgg aaaggcgaaa ctgaaactga gacggaaaca gtcaaatggg aaaaaggaat
    20161 gagaatcaat cccgatttcg ccatcaaaaa agaactatct gcaccgccta aaccattgtc
    20221 agaagcaagc ttgcttggtc aaatggaaaa atacagtctg ggaaccccag ctacgcgtgc
    20281 agagattatc gagaaactaa tcaaatctga actgatggaa cgtacccctc aaggactgca
    20341 agtatctcct aaaggtaagc aattattagt attggtaaat ccctctttag taacaccaga
    20401 tttgacagaa agctgggaaa aagaccttga agctattgct gcaggaaaga aacaagcagg
    20461 aaccttcttg aatggaatcg aaaaagaaac aaaacgcttg gtgaatgaga tcaaatcaag
    20521 caaacaagaa tatcaagatt tctcgattac ccaaaagaaa tgcccagaat gtggggccaa
    20581 tctgcgcgag aaaaacacac gtgacggcaa aatctatgtc tgcaccaatt cagattgcaa
    20641 ctaccgcaga agaaaagatc caaaagtctc taatcatcga tgcccgcaat gtcaccggaa
    20701 aatggaaatc atcgaaggta aaaacggtgc ttatttccgt tgtaaatacg atggaacgac
    20761 agaaaaattg cttgataaaa aagagcagaa gaagaaaatg accaaacatg aagaacgacg
    20821 cttgctgaaa aaatatgcac aagcagaaga accagaagaa agcccattag cagcagcact
    20881 aaaagcagca atgaagaagt aataagatat gaaagaggtt gtgatagaag tggtcagctt
    20941 caagaaataa gaaggaattt acgaaaattg cttctcaaat ttttgtgaaa tttcggctta
    21001 tttccgaaga agctacttct ggaacaccat ttattcggaa aaaaggactg tgacaagact
    21061 tttgtcacag tcctttttca tttaaaaaaa tgtttacttc atttgacgta tattattggt
    21121 gaggcttcaa ttcatttggc ttcacttttt ctgtttctct tatgatataa acaggacgct
    21181 gtttcgtttc taagaagatt ttcccaatat atttcccgat gattccaaga cataacaatt
    21241 ggattccacc aatgaacagg aagatcgaga ccatggaagg ccatcctgat gtaggatcac
    21301 caaacaacaa tgcccgcaaa ataatgaaca aaattgcaat accagaaccg acacaagaca
    21361 aagcgccgat aaatgaagca atcgttaacg gtgcatccga gaagttcacg ataccatcaa
    21421 tcgagtagtt gaataatttc cagaaggacc aagatgtttc gccagccaca cgttcgcggt
    21481 tttcataaga aatatattct gttttaaatc cgacccagct aaataatcct tttgaaaatc
    21541 ggttgtattc tgttaattct aggacggcat ccaccatttg acgagtcatc aagcggaaat
    21601 cacgagcgcc atcgaccatt tctgtatcgc tgattttatt gatcaactgg tagaattttt
    21661 tcgcaaagaa actgcgtatc gcaggctcgc cttcacgagt cgaacgccgg gtgccgacac
    21721 aatcgatatc agaagtagtg atccggttga tcatctcagg aagcagttcg ggcggatctt
    21781 gcagatcaac atccatcacg gtcactaaat ctcctgtaga agcttgcaat ccagcgtaca
    21841 gagccgcttc tttcccaaaa tttctggaaa atgagacaaa gcggacataa ggataagctg
    21901 aggctagctt cttgattgct gctaacgtac cgtcttttga tccgtcgtca ataaacaaat
    21961 attcgaactc atgggcacct ttgaattttt caacctcatc aaaaaaaaga ggaacagttt
    22021 tttcttcatt aaaacatggg acaacaattg atactttcat cgtttctatc ctttcaatcg
    22081 tatgtttata tatggtttac attcaaaatt taataaacct taaataacac ctttaaaatt
    22141 tgattttacg taaaagaaca ggtattcttt ttgtaaacta acgtatttag tttaccatgt
    22201 ttcctcggtt tatcaaactt ttaagaacaa tttttattag ttggcaagta gttttggata
    22261 gagaaaaagg aggaagttcc agtctattga ctaggagcaa acaaaatgaa aatacacaca
    22321 cctatcggct ttttatggtt agatgcagat gaatccggat tgactgctgt atcttttcag
    22381 ccaatttcag aaagcgaatc agcgaatcag cgaattttaa cgcgcacaca agaacagttt
    22441 gaagaatatt ttgcgggaaa aagaaaagaa tttgatgttc ctttatctat aaagagaggt
    22501 acgccttttc agcaaaatgt ctggaaagct ttgtcgacca ttccttatgg agaaatccga
    22561 acgtataaag aagtagccgt tgctgttgat agtccgaaag ctgtgcgagc gattgggcag
    22621 gcaaatcgaa ccaatccatt gccaatcgtt attccttgtc atcgagtgat cgggcaaaat
    22681 ggaaaactga caggatatat gggaaacgca atagatgggg ttgaaatcaa acgccaattg
    22741 ttgttgttag aaggatatct gcaaaattaa aaacagttgt gattcggaca ttgttccatg
    22801 cgagaatcac aactgttttt tcatgttcat tctaaacttt gatcagccaa ataaatagta
    22861 aagccatcac tgtttggaga agcccgacag ctaatccata agataagaag cgtgtacctt
    22921 ctttcatgaa ttttgcaaag tctaaacgca gaccaattgc cgctaaagca gtgatctcaa
    22981 accagctgct gataaaatgt gccgattggt caataactac aggtaaagaa aacaagctgt
    23041 tgaccaaaca aaagatcaca aaccctaata cataccaagg aagcggtaat tttcgttttt
    23101 tctgcgtatc agttggcgtt tcaaacagag ctggttgttt cttgtgcatg aatttttcaa
    23161 acagatagac aacaacaacg agcatcataa tacgtagaat cttaaatagc atggcgaatt
    23221 cgacaacaga attgtcaagt aagcttgccc cagcgactac ttgaccaaca gattggagtg
    23281 taccacccaa aagtgcactt ttttcaatga gatttccacc aaacaactgt aatccaagaa
    23341 aagggagagt caacatcatc acagtaccca gtagattgac tagtgtgatg atctgtcctt
    23401 tttcttcctc tttcgcatgg atggaagggg cgattgaagc aatagcagaa gaaccacaaa
    23461 cagcgtttcc tccagccatc atcaaagaag aagtatcgct gaattgcagt tttttaccaa
    23521 tcaagtaagc accgatgata gtgactgaca tttgcagtaa cacaaaaaca attcctttta
    23581 caccaatttg agcaatcgtt tgagctgtga cagttgcacc tagtaaaaaa acagagaatt
    23641 ctaatagttt gctttcagca aattttgtgc cgattgctag aacaggctgt ttaaaaaaag
    23701 tatttcctaa taaaataccc aataaaatag caatagtcgc tccacctaac tgtggcagat
    23761 aaatagcaag aagcctgctg aaggcagcga tggcaaaaga aagcagtaaa ccgggtaaga
    23821 tttcgcggac cgctgaatcc tttacagatt ttattggatg tgacaatgat ttcatttgat
    23881 cggttccttt cgtcaataaa tactaaaagc atactcgaac atagtagcac taaaccagta
    23941 ttttgacaaa agaacaagag gtcgtgacaa aagtcttgta cgacctcttg tttcgaataa
    24001 acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa atttgagaag
    24061 caattttcgt aaattccttc ttatttcttg aagctgacca cttcttttac gacctcttgg
    24121 taaacggtgt tcaaaagctt gcatctgcgg taataagccc aaccagtaca aaaacatgaa
    24181 cagctgtttt tgtttggccg ttcttattac ttgatgcaga acagcttttt cacaacctat
    24241 tgaatcgatt attaagcgat ttttttagaa acaaattttc catgtttgct atgttcttta
    24301 attaagaaac gtacgtaatg agcaagaacg tagaagacaa ctacacttgc aaacagacat
    24361 tccgcaactt ttaagaaatc caatacgcta ctatagatac ctaaactgtt gaagtaatga
    24421 acagtagtaa atatagctgt tgcagagata acaagcggga atgtaaaggc tgcatagctt
    24481 ggataaaaac gaagcttgaa caaacgtgga agcatagaca gcacaacgaa atacaatact
    24541 tgagataaag cgaacaatgc tacgacaaga taaaaattag gttcagcaaa gtttttaaga
    24601 taacctgtta ggcaaagaga accaggtgct gcaacgatcg taatcagcgg acgtgtcgga
    24661 tgagccatat ttcttacgaa aaagacacgt ttgatgatga tcggcaataa gaacgcatag
    24721 cagatcaatg aaatccaaaa gaaaattttt ccaataagtg ggaagaagtt cccggcagtt
    24781 acagtgatga ccccaaatcc aacgtaaatg ataaaccagc ttggataaat tgcttcaatt
    24841 gaaagatcag ctttgataac atggtgataa ttgaaataaa agacaaggat cacttggaag
    24901 ataacagcgc acagccaaat gaatttgaca attggtgcaa tactttcata tgaaacaaaa
    24961 taagtacaaa taaccatgag tgacatcgta aaagttggtg aaacggaggc gatgattgga
    25021 tcatgcaacg tttgtttcgt gtgttcgaat aagataaaca atttaacaaa aatgaatatc
    25081 ataagaatca tagagatacc gccagttaga ttgcctaaag caacatggtg atagtcttta
    25141 aacagatttc caagggaagc taacccaagg atcaacccgc agatcgggat cggaataatt
    25201 ttcaaaaagt tcttcatata taatattctc ctttttattt aatagatttt agtaccaaga
    25261 tgaacgaata cattgtaaaa tgaataggaa tgtatgatta cgagcaagca gacagctgat
    25321 tgtcttcatc taagttacta gttggatcag caactgtttt gttgtacatt tctattatag
    25381 atggtttttt gtatatttaa atcaaatagt ttttatagta attatcataa aaagttatgg
    25441 aagtggtgcc atgtttaagt tattaacgac ttttcgagca gtatatgaaa caagaaattt
    25501 ttccagagca gcagaagtac tgtttttatc tcaaccagca gtctcgaatc aaatcaaaca
    25561 gttggagcgg gaattgaata tcgatctttt tcaacgtaac ggaagaaaag agatgcttcc
    25621 aactaagcaa gccgacttat tatatgaacg gacactggtt ttgttagaag aatgggaaga
    25681 aactcagcaa aaattgctca atgaacaaaa tgaagaagaa gtatgtcgga tcgttgcttc
    25741 ccacaccttt gctgtctatc ttttgcctca gttgatgaaa catctgatca aaaggtttcc
    25801 taaagtgcat tttgagatcg aaatcacgaa ttctcatgaa tcattagaac aagtagcgaa
    25861 acatgaatcc gattttggtt ttatcgaaaa gccattagag acttccggcg tacaaagata
    25921 ctctttgatg gaagaccagc tagtcatcgc tggggaccca aaaagcaaat tgtggcttgt
    25981 aagagaagca aactctggtg tttttcatta cacgcagcgt tattttgaag agatgaatat
    26041 ccaagataag aagatgatcg tgaaaagtaa cgatgtaatt gttgcgttgc tgaaggaagg
    26101 aatcggtcgc tcgattttgt ctaagcgctc tgttccagaa aatgtgccaa tgactccgtt
    26161 gaatttccat cgatattttt atttcatcca acgaaccaat ctggtttctg atcagctgct
    26221 agagattgcc gaagagatcc ttcgatacaa tgaaagcaca gaaacttctc acagcgagga
    26281 gaatctcagc tggtagataa ttgcatgtat caaaaaaagt tctatgatac actaaagata
    26341 agcagtaggc ttttgtactg catttgctca agcaactgca caaacggaaa gttaattttt
    26401 cccaaatgtc tatggaaaga ctttccatta tctaactgga ccgctataat aatcagcagt
    26461 atggttttta agaaaaggga tgaaacatgg aaaaaaaatt aaaagcttcg aacgataatc
    26521 gtattgatta tggcgtgatt ttaccagtct ttctgttatg tttgattggt ctgctctcat
    26581 tatatgtagc acttagccat gatccgaaca atccagcagt cttgaaaggc gtagggatgc
    26641 aagttgtctg gtatttagtt ggagcggcag caatcgttgt catcatgcat ttgaattcca
    26701 aatggatctg gaaactgact ccttaccttt atggtttggg gttagtggta atgggactct
    26761 tgctcaaatt ctatgatcag aatttagctg cttcaacagg gtctaaaaac tggtttcgat
    26821 tcggtgcgtt tactttccag ccagcagagt tgatgaagat tgcgtacatc ttgatgatgg
    26881 cactgatcgt aactaaacat aatacgcaag taaaagaacg gacgatgaaa tcggactttt
    26941 ggctgattgg gaagctcttg atcgtgacaa taccggtttt agcattgatc atggcccaag
    27001 atgactttgg aacgatgctt gtctttcttg cgatctttgg cgggattttc ctgatgtctg
    27061 gaatctcttg gcgcatcatc gcacctgtag taatcttggc agtagtcgtt ggagcaggaa
    27121 cgatcttctt ggttacgact gaaggcggaa gagacttgct ttataaagtt ggattcaaat
    27181 cttatcagtt tgcacgtatt gattcatggc tggacccttt ccatgatacg tcaggaatga
    27241 gttatcagcc ggcccaagga cttttggcaa ttggtaccgg tggcttgttt ggtaaaggat
    27301 tcaacgtaag caatatctat gtgcctgttc gtgagtccga tatgattttt accgttatcg
    27361 gtgaaaactt cggctttatc ggtggtgcat tcgtcatttt cttgtatttt attttaatct
    27421 atcgtatgat tcgggtgtgt ttcgatacga ataatgaatt ttacgcctat atcgcaagcg
    27481 gtttgatcat gatgctgctg ttccatgtct ttgagaatat tggagcaaat atcggattac
    27541 ttcctttaac agggatccca ttgcctttca ttagtcaggg aggatcctcg attttaggga
    27601 acatgatcgg tatcgggttg atattatcga tgagatacca aaatgaagca cctgcaagat
    27661 cttcaaggag gtaagaaaat tgtacacctt ttactggtac ccgaaatgtt caacatgcaa
    27721 aaaagccaaa gcgtggttag accagcatgg cgttagttat caaaccgttg atatgatcaa
    27781 agaagtaccg aaggcctcaa cgattgagac ttggctagaa acgagtcaag taccaatccg
    27841 tcgcctgttc aacacaagcg ggatgaaata tcgggaactt ggactgaaag atcaagtagg
    27901 cgagtttacg gcttcaaaag cgagccaagt cttatcgaca gatggtatgc tgatcaagcg
    27961 accactgatc ttacgagagg accagcttgt tgcaattgga ttcaatgaaa aaatttatga
    28021 aggagaactg atataaaatg gcaggcaagc atttgaagaa aaaggacaac ctttggatct
    28081 tatttaatgg aaaagaatac tgtgttggtt tgacaaatga agcgcaagaa gaacttggag
    28141 acatcacgtt tgccaatctg ccaaaagcag gacaatcgct aaaaaaagat gaacctctga
    28201 tcgaagttga agcggaaaaa gcggtcaatg aatttgccag cccgctaacg ggtacagttt
    28261 cttctgttaa tgataaaatc ggtgaagata tcaatatctt gaatgattca gatgaaatga
    28321 atgcgtggat cttaagtttc aaagatgttg atccaaaaga atttgacgaa ttataaaatc
    28381 agaaaatcac ttgaaaaaag ttgacactga ttcttccagt tgctataatc ataagcaatc
    28441 ctatatgaaa cgagaaagct ttgaaaagaa gagtacatgt cacggacgtt tgaagagagc
    28501 cctgtttgct gaaaatgggt aacgaaaatg agatggaaga tggtcttaga gcagagtaag
    28561 cgagcgtaag caaacttact acgggagtgc ccgttacagc gcgacagtat acggaaagaa
    28621 gctaagtcca agtactggaa gaggctgtta ttgcgaagta acggtaaatc aaggtggtaa
    28681 cacggaagtt caagctttcg tccttttgct taaggcaaga gggcgaaggc ttttttgttt
    28741 gaaaaattat aagagaaaga tgtgaatggc atgtcattaa tccaactaac gaatgtcaaa
    28801 aagacattca atgggaaaaa cggaaacatc caagccgtca atgacgtttc actaaacgtt
    28861 gaaaaagggg atatttatgg aatcgtcggc tattctggcg caggaaaaag tacattagtc
    28921 cgtctcctga atggacttga gcttcccacg agcggcgaag tgatcgtaaa cgatcaagat
    28981 attacaaaat taaaaaacaa agaattacgt acattccgaa agaaaatcgg tatgatcttc
    29041 cagcatttca acctgttatg gtcaagaact gtacttgaaa atatccagct gccgctggaa
    29101 ttagctggcg taccaaagag caaacgtaaa gaacgggcag aagaactgtt gcgtttagtc
    29161 gggttagaag gacgcggaca agcatatcct tcacaacttt ccggcggaca gaaacaacgc
    29221 gtcggtatcg cacgagcatt ggcaaatgat cctgagatcc tgctttgtga tgaggctact
    29281 agcgcattgg acccgcaaac aacagaagaa gtattagact tattgttagc gatcaataag
    29341 aaattgaatt tgaccatcgt cttgatcaca catgaaatga atgtgatccg caaaatctgc
    29401 aacaaagtcg ctgtgatgga gttggggaaa gtcgtagaag aaggagacgt attgactgtc
    29461 ttcagacaac cgaaaaaaga agtaaccaaa cgctttgtac aacaagacat cgaaccagaa
    29521 gctgattcga cagaactatt agctgaactc atcaaagaaa atccaagcgg ccgtttggca
    29581 actttgacct ttagcgaaag aaatgccaac gagccagtca tttcccaagc gattcgacgc
    29641 tataacatcg atgtcaacgt tgtgtatgga aagatcaaac aagtgaaaga aggttccttt
    29701 ggttcattga ctgtcttgat gactggtgaa accaaagaat tagatcaagc agaagtgttc
    29761 atcaaagaac aaggagtagg aattgaggtg atccatcgtg gataaaagct tttcagaaac
    29821 atatctagat tttaaacaaa tcaacccgga aacgatgaat caggctatcc ttgatacact
    29881 atatatgaca ttaatctcaa tggtcttagt gacgatcatc ggattagttt tgggattgat
    29941 cctttactct attggacgca aaaacaaacc gtatgctcga atcatttatg gaatcgtatc
    30001 gatcatcagt aatattttcc ggtcaacgcc atttatgatc ctgatggttt tgatcattcc
    30061 atttactaaa gcaatcgtag gcactatgct aggtgcgcaa gcagccattc cagctcttgt
    30121 tttatccgca gcaccatttt atgcccgtct agtagagatt gcttttcgtg aagtcagccc
    30181 tggtgtactg gaagcagcag atgcaatggg agcaagttac tgggaaatca tctggaaggt
    30241 cttgatacct gaaagtattc ccgcattgat ttctggatta acagttacaa cgatctcgat
    30301 gatcggattt actgcgatgg ccggcgcgat cggcgcaggt ggacttggag gtcttgcttg
    30361 gcaggaaggc taccagcgtg gtaatctaac agtgacattc gttgcgactt taattatttt
    30421 actaatcgta tttatcgttc aaggaatcgg tgatttctta actaagaaaa cagacaaaag
    30481 ataataggat atagggggat gtaggatatg aaaaagaaaa tttttggatt tgcagcagcg
    30541 cttttgctga ctgtaggatt agcagcatgc ggtaacaaca gcgacagcaa agattcagca
    30601 aataaagcag acgacacgac attgaaagtc ggcgcttcac cgacaccgca cgcagagatt
    30661 ttggaacatg tgaaaccatt attgaaagat gaaggaatcg atcttgaaat cgtcaaattc
    30721 gatgactatg tattaccgaa ccaatctttg gaagaaggcg acattgatgc caattacttc
    30781 cagcatattc cttatttgaa caaagaaatc aaagaaaaag gatatgactt tgtcaatgcc
    30841 ggtgctgtcc acatcgaacc aatgggcttg tactctaaaa aagtcaaaga tgtttccgaa
    30901 ttaaaagatg gtgcaacaat cattacttca aattctgaat cagactgggg acgcatcatc
    30961 acaattctac aagatgctgg cttagtcaaa gtcaaagatg gagtagatct ggaaacagca
    31021 acattcgatg atattgaaga aaaccctaag aacttgaagt tcaaccatac gatcgaccca
    31081 tcacttttag cttctacgta ccaaaatgat gaaggtgacc tagttgcaat caacgcaaac
    31141 ttcgcttacg gcgcagggtt gaatccatta gaagatgccg tattgcttga aaaggacaac
    31201 tctccttacg tgaatattat tgcgactcgt aaaggtgatg aagacagtga caaaatcaaa
    31261 aaactgattg aagtgcttca ccgcaaagaa gtccaagaat ggatcacaga caaatggggc
    31321 ggctctgtta aaccagtaaa ggctgatgca aaataaggta tggaacaaaa aagatgagaa
    31381 ataagtctgt aatggcttat ttctcatctt ttttatacct attctaattt agaaatcaaa
    31441 attgtttaat aataaataaa agatatttga ttttagtaag gaaggtcatt gcgcttcatt
    31501 ctcaattagt ctaaaatgag tgagaacgaa agagaatttg ggggtataat gcatgtctgt
    31561 cttagaaata aaaaatctac atgtgtcgat cgaggaaaaa gaaatcctta aaggcgtaaa
    31621 cctaacaatg aaaaccggag aaatccatgc catcatggga cctaacggca caggaaaatc
    31681 taccttatct gccgctatca tgggcaatcc aaactatgaa gtaacagaag gcgaaatttt
    31741 gtttgacggg aaaaatgttt tagacttgga agtagatgaa cgtgcgcgac ttggcttatt
    31801 tttagctatg caatatccaa gtgagatacc aggtatcacg aatgcggaat tcatgcgtgc
    31861 agcgatcaat gcgaaacgtg atgaaaacga caaaatttct gttatggcat ttttgaaaaa
    31921 gttagatgaa aaaatggctt tactaaatat gccagaagaa atggctgaac gttatctgaa
    31981 cgaaggtttt tctggcggag aaaagaaacg gaatgagatc ctgcaattgt tgatgttaga
    32041 acctacattt gcaattttag acgaaatcga ttcaggtctg gatatcgatg ctttgaaagt
    32101 cgttgctaaa ggagtaaatg aaatgcgcgg agacaatttc ggtgcattga tcatcaccca
    32161 ctatcaacga ttattgaact atatcacacc tgatgtcgtt cacatcatga tggaaggccg
    32221 agtagtcatg acagggaatg ctgacctagc taaacgtctg gaagcagaag gctacgctgg
    32281 tatcagtaaa gaattaggaa tcgactataa agaagaagaa gcgtaagggg gagcaagaat
    32341 gaaagaaaaa aactggctcg attatcttga cgcggtaaat gatttttctt taagtaaagg
    32401 cgaacctgat tggatgagaa ccttccgtca agacgcacta gcgaaagcag atgaactgcc
    32461 attgccgcat atcgatcggg tgaaattcca tcgctggtcg ttatttgatg tgaaagaaac
    32521 acaaaccatc agtgaaacag gcacgattcc ggcatttgat gcaatgaaag acaatcctgt
    32581 gcttgtccaa caaggttcat ggacgatttt tgaacaactg cctgtggaat tagcagaaaa
    32641 aggagtcatt tttaccgact tgttcactgc aatgatcgaa tatccagaac tagtccaaga
    32701 atattatatg aaaaaagcag taaacatgaa cgaagaccaa ttgactgcac tccatgtagc
    32761 ttttatgaac agcggtattt tcctgtatgt gcctaaaaat gtcgtgatcg atgaaccact
    32821 cgagtcctta ttcattcaag atggcgcatc cgatgagcac ttcttcaagc atgtgttgat
    32881 cgtggcagat gaacatagtg aattttctta tctggaacgt ttccagacaa ccaaagaaca
    32941 agtagcaaaa tcatcaggta atatcatcgt ggaagtcatc gctaaagctg gttcaaaaat
    33001 caaatattca gccgtagatc aattaggaga aaatattact tcttatatga atcgccgtgg
    33061 gcacatttta cgagatgctt ctgtcgactg ggcaattggt gtcatgaatg acggtcatgt
    33121 gatcgctgac tttgactcag atttagcagg agaaggtgca cacgcagaag tcaaaatcgt
    33181 ggcaatcagc tcgggcagac aaatccaagg aatcgataca cgagtaacca ataaagcccc
    33241 tcatacgatc gggcatattt tgcaacacgg agtgatccga gaaaaaggaa cattgacctt
    33301 taatggtatc ggccacatct tgaaaggggc aaaaggtgca gatgcccaac aagaaagtcg
    33361 tgtcttgatg ctttctgata aagcacgtgg tgatgcgaat cctattcttt tgatcgatga
    33421 aaacgaagta actgctggac atgctgccag tgtaggacga gttgatccag aagaaatgta
    33481 ctatctgatg agtagaggat tgcataaaga agaagcagag cgcttagtga ttcgaggatt
    33541 cctcggttct gtcttaacag caatccctgt agaacaagtt cggaaagaat tagtcgaagt
    33601 aatcgaaggg aagttgaatg gatgatcgat gctaaaaagc tacgcaatga ttttcctatc
    33661 ttatttcaag acgtcaatga tgaaccttta gtttatctgg ataatgcagc gacgacccaa
    33721 aagccaaaac aagtactcca ggcattagcg tactattacg aacacgataa cgccaatgtt
    33781 catcgcggtg tccatacgtt agcagaacgc gcgacccatg attatgaaca agcaagagaa
    33841 aaagtacggg cgttcatcca tgcaaaagaa acagcagaag tgctgtttac tcgtggtacg
    33901 acgacaagct tgaattgggt agccagaacc ttcggcgacc gctatatcaa agcaggagac
    33961 gagatagtgc tctcttacat ggaacatcat tctaatgtta tcccgtggca gcagttggca
    34021 aaacgaacag gtgctgtctt gaaatatatc gaactaacag aagaaggctt cctggatatg
    34081 gaggatgcag cgaaaaagat tacggacaaa accaaaatcg tctccattgc tcatgtatcc
    34141 aatgtcttag gtgtcttcaa tcctgttcaa gaattagctg aacttgtcca tgaaaacggc
    34201 ggggtcatcg tagtagatgg tgctcaatct gccccgcaca tggccattga tgtccaagca
    34261 ctagactgtg atttctttgc tttcagcgga cacaaaatgt gcggaccaac aggtattggt
    34321 gttttatatg gtaaacgaaa atggttagaa gaaatggaac ctgttgaatt tggtggcgaa
    34381 atgatcgatt ttgtcgaatt atacgacagt acatggaaag aactaccttg gaaattcgaa
    34441 gcagggacac caaatatcgc cggcgctatc gcattaggcc atgcaatcga ttatttgcaa
    34501 aaactcggta tggacaatat ccatcgatat gaagaagaac tagccgctta tgtgttgcca
    34561 aaattacaag caattgatgg attgactgtt tatggtccac aagacccgaa ttttcggaca
    34621 ggagtcgttg cattcaattt ggatgggtta catccccatg atgtcgcgac agctttagac
    34681 atggaagggg tagccgtcag agcaggtcat cattgtgcgc aaccgctatt gaagtacctt
    34741 gaagtatcag cgactgctcg cgcgagcttt tatttctaca acacaacgca agacgccgac
    34801 cgtttagttg aagcaatcat ggcaacaaag gagtttttcc aacatgggac tatctaaatt
    34861 agacaattta taccgtcaag tgatcttgga tcattcttcc catccgcatc atcgtggaag
    34921 cttgactgac tcgacaagca agatcgaatt gaacaacccc acatgtggag atgtgatcga
    34981 attgcaagtc gatgtaaaag acggaatcat caaaaatatc gcctttgacg gcagtggctg
    35041 ctccatcagt acggcaagtg cctcgatgat gacagacgcc gtcatcggaa aatcaataga
    35101 agaagcagaa catttaactg tagactttct acagcttgtc caaggaaaag aagtcaaaga
    35161 tcccgaccaa ttaggcgatg ctgctatgct tggtggtgta gctaaatttc ccgctcggat
    35221 caaatgtgcg acacttgcct ggaaagcact aggaaaagca atggaaaacg atggtaccgc
    35281 agctttccat cattaccaca aggaagagga gtgagaacat gggcgtacca gaattagaag
    35341 aatataaatt cgggtttcac gatgatgtcg aaccggtttt cagtacagga gaaggcttga
    35401 cagaagaagt gatccgtgag atgtcaagaa tcaaaggcga accagaatgg atgcttgaat
    35461 tccgtctgaa atctctagaa acatttaaca aaatgccaat gcaaacatgg ggccccgacc
    35521 tttcagacat cgatttcgat gccatcaaat attaccaaaa accaagtgac aaacccgctc
    35581 gtgactggga agacgtacca gaaaaaatca aagatacatt tgaaaagatt ggtataccag
    35641 aagctgaacg agcttatcta gccggtgctt ccgcacaata tgaatcagaa gtcgtgtacc
    35701 ataatatgaa agatgaattc gaaaagttag gaatcgtctt tactgacact gactctgctt
    35761 tgaaagaata ccctgaatta ttcaaagagt acttttcaaa acttgtaccg ccaaccgaca
    35821 acaaattagc agcgctgaat tcagccgtat ggtcaggtgg gacctttatt tacgtgccaa
    35881 aaggcgtcaa agtcgatgtt ccattacaaa cgtacttccg gatcaatgcg gaaaacacgg
    35941 ggcaattcga acgtacgctg atcattgttg acgaaggagc aagcatccac tatgtcgaag
    36001 gttgtacggc acctacttat tcaagtaaca gcctgcatgc cgccatcgta gaaatcttta
    36061 caagaaaaga tgcgtattgc cgttacacaa cgatccaaaa ctggtcagac aatgtgtaca
    36121 acttagtaac caaacgagca aaagcatacg aaggcgcaac cgttgaatgg atcgacggaa
    36181 acctaggtgc aaaaacaacg atgaaatacc caagtgttta cttagatgga aaaggtgcac
    36241 gaggcaccat gctctccatc gcctttgcag gagcaaacca aatccaagat acgggtgcaa
    36301 aaatgatcca caatgcacca aatacatcta gctctatcgt ctctaaatca atttcaaaag
    36361 acggcggaga agtaaactat cgaggacaag taacctttgc caaaaacagt gcgggttcga
    36421 tctctcatat cgaatgtgac acgatcatca tggacgaatg gtcgaaatcc gatacgatcc
    36481 cattcaatga gatccacaac agccaagtct ctttagaaca cgaagccaaa gtctcaaaaa
    36541 tctcagaaga acaactctac tatctgatga gccgaggcct gaccgaatct gaagcaactg
    36601 aaatgatcgt catgggcttt gttgaaccat tcaccaaaga acttccaatg gaatatgcag
    36661 tcgaactcaa tcgattgatc agttatgaga tggaaggcag tgttgggtaa acctgttata
    36721 ggagctagaa cccttgatat ataagggttc tagctttttt gttttgcctg tatactagaa
    36781 atgaccagat tttgaccagc tcttttactg atgagccatc atatcagcaa agtcttgacc
    36841 tgttttctta ggtgcattat ttgtaatatg tgcatagatg tctaatgtca ctttactatt
    36901 ttcatgccct aatcgttgtt gaactacctt gacagattct cctgcttcta ataataaact
    36961 agcatgtgta tgtctaaacc catgaggtgt aatacgaggc aagttatatt ttttcaaaat
    37021 atatcttaac caatcattaa cagtgtttgg aatgtataga gtgtttttaa ctgttgtaaa
    37081 aacatgttgc tctttagagc tagtattgta tccaagtttt aaatactctt ctttttgctg
    37141 taatttccag ttgttgagga tagaaagagt ctcagtatct aaagagataa ctcgtctaga
    37201 agctcttgtt ttcggagatt gaattataac tttaccaaat tcatctacag caagagtttt
    37261 attgacatgt agttctttat taaacgtgtc tatgtctttc cactgtaaag cgagtacttc
    37321 actctttctc atacctgtgt atgccaataa tctgaagaag gcgtattgtt tcatgttacc
    37381 aaaatcttta aaagcatcca gtaaattatt aagctcctct tttgagtaaa atttacgatt
    37441 tttctcatac tctttttttc gtggaagaag tgttttttcc ataggattat tgtcaatata
    37501 ttgcattgca accccgtagc gtaaaatttg tgcagtctgt tttctcatat aatcgtaact
    37561 ttcatattta ctgtgccatt catctaccac tttttgacaa tatgggacag agatattcga
    37621 tagtttaagc ttaccaaatg ctggaagtat ttgattattt gcgtatctta ctgagattgc
    37681 gattgttgaa gctttaacac tggtacgctg atgagttatc cattcattat acaggtcttc
    37741 aaatgtcata tcaggtgctt tctttttaac accgtataat atttgatgtc tgtactcgtc
    37801 ataatctttt tgagcttctt gtttgctact aaaacctctt ctcgtagtat ttattttttc
    37861 accagttcgt ggatcggtgg caatgtgtcc tttatacatc cacgccactt gaccgttttt
    37921 ctttttgtac tttgatattt ttgccatgaa atcatctcct cttagtttac aaggagtact
    37981 taccactaga tggtatcacc tcccttcatt tttttcttag tgtatttttt cccaattgat
    38041 gaaatcgttt gttctagctt gtttatatta tcagtaattt ctctcacagc ttcttcggga
    38101 taagacatgt ctttcaattg aataggtagc cattcatcta ccattctttc tagtagttct
    38161 tctatactgc tagtgaggaa aagaatgtta ttgtcaaatt cttctacaat agcattgatg
    38221 aataattctt ctatttttaa gatattgaaa agactaattt tttcattttt aactttatta
    38281 caagtcttgt tagcaataaa ttcttgcatc tctggttcta atttggaata tctatccagc
    38341 catgtatcta aattagagtg tactggtggg aactgttgac gaagatgctg gaacaggttt
    38401 ttatctggaa atgttatacc atctagtaca atgctatctc tataaagaat aattcccaaa
    38461 atatattctt ctacattgcc acaaactaac tcctctatac tcatatttcc caaatcagca
    38521 attcgggata atcgctctgg tattggtaaa taggttccct tctcccattc gtagatagtt
    38581 tttttgtcaa ttaattgacc gttgttaatc ataagaccaa atgctggttg tgttaatttt
    38641 ttactctttc ttatagcttt tatttttgtt ccgagtatct tttttcgttc taaataattt
    38701 attggtggac gttttatcat attgaccaca cctcctaaaa ttagtatacc tcgaaaaagg
    38761 ggtgttgtaa ataaatttta caaagatatt gacacctctt ttttatcgag gtattatgtg
    38821 tatgtcgatg aactggtaat cattttttgt ttgattaatc ggttaataac aaaatacttt
    38881 tattttgagc aaaaaatacg aagataattc ttagaaaatt tgttcaagca atcacgaaag
    38941 gagggggaaa tatgtctcta caaatcattg atagcattgt ttctatacaa gttgaacaac
    39001 aagtgaaaga gataattaca gtgctaaagc ctatgctaga agacattatt tctggagtca
    39061 ctctagagta tatggactat cagcaggcaa gtgaatatct aggtgtatca gtaggaacga
    39121 ttagaaaata tgtttctcaa tacgggttac ctgttataaa aattgatacg gtcattagat
    39181 ttaaaaaatc tgatattgat agtttcatgg aacggtataa aggttgagta aacaaataag
    39241 actaaaacat atagtaataa agagtttgtt tttattatct gtatgtttta gtctttaaca
    39301 gaattttata aggagg
//
LOCUS       DAYYGO010000011        37327 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000011 DAYYGO010000000
VERSION     DAYYGO010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37327
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.011"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(226..2379)
                     /locus_tag="ACHA5T_000509"
     CDS             complement(226..2379)
                     /locus_tag="ACHA5T_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFM8886113.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(2673..3521)
                     /locus_tag="ACHA5T_000510"
     CDS             complement(2673..3521)
                     /locus_tag="ACHA5T_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8886114.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(3745..5685)
                     /gene="thrS"
                     /locus_tag="ACHA5T_000511"
     CDS             complement(3745..5685)
                     /gene="thrS"
                     /locus_tag="ACHA5T_000511"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFM8886115.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(6022..6201)
                     /locus_tag="ACHA5T_000512"
     CDS             complement(6022..6201)
                     /locus_tag="ACHA5T_000512"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFM8886116.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(6248..8071)
                     /locus_tag="ACHA5T_000513"
     CDS             complement(6248..8071)
                     /locus_tag="ACHA5T_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8886117.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            8349..10628
                     /gene="helD"
                     /locus_tag="ACHA5T_000514"
     CDS             8349..10628
                     /gene="helD"
                     /locus_tag="ACHA5T_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFM8886118.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(10721..11368)
                     /locus_tag="ACHA5T_000515"
     CDS             complement(10721..11368)
                     /locus_tag="ACHA5T_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886119.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIDWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(11547..12266)
                     /locus_tag="ACHA5T_000516"
     CDS             complement(11547..12266)
                     /locus_tag="ACHA5T_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8886120.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(12397..13548)
                     /locus_tag="ACHA5T_000517"
     CDS             complement(12397..13548)
                     /locus_tag="ACHA5T_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFM8886121.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(13765..14505)
                     /locus_tag="ACHA5T_000518"
     CDS             complement(13765..14505)
                     /locus_tag="ACHA5T_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8886122.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(14509..14832)
                     /gene="rsfS"
                     /locus_tag="ACHA5T_000519"
     CDS             complement(14509..14832)
                     /gene="rsfS"
                     /locus_tag="ACHA5T_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFM8886123.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(14889..15482)
                     /gene="yqeK"
                     /locus_tag="ACHA5T_000520"
     CDS             complement(14889..15482)
                     /gene="yqeK"
                     /locus_tag="ACHA5T_000520"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFM8886124.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(15466..16116)
                     /locus_tag="ACHA5T_000521"
     CDS             complement(15466..16116)
                     /locus_tag="ACHA5T_000521"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFM8886125.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(16126..16437)
                     /gene="yhbY"
                     /locus_tag="ACHA5T_000522"
     CDS             complement(16126..16437)
                     /gene="yhbY"
                     /locus_tag="ACHA5T_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFM8886126.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(16453..17562)
                     /gene="yqeH"
                     /locus_tag="ACHA5T_000523"
     CDS             complement(16453..17562)
                     /gene="yqeH"
                     /locus_tag="ACHA5T_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFM8886127.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(17559..18086)
                     /locus_tag="ACHA5T_000524"
     CDS             complement(17559..18086)
                     /locus_tag="ACHA5T_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFM8886128.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(18266..19051)
                     /locus_tag="ACHA5T_000525"
     CDS             complement(18266..19051)
                     /locus_tag="ACHA5T_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFM8886129.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(19044..19913)
                     /gene="accD"
                     /locus_tag="ACHA5T_000526"
     CDS             complement(19044..19913)
                     /gene="accD"
                     /locus_tag="ACHA5T_000526"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFM8886130.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(19914..21293)
                     /locus_tag="ACHA5T_000527"
     CDS             complement(19914..21293)
                     /locus_tag="ACHA5T_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFM8886131.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(21301..21720)
                     /gene="fabZ"
                     /locus_tag="ACHA5T_000528"
     CDS             complement(21301..21720)
                     /gene="fabZ"
                     /locus_tag="ACHA5T_000528"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFM8886132.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(21745..22221)
                     /gene="accB"
                     /locus_tag="ACHA5T_000529"
     CDS             complement(21745..22221)
                     /gene="accB"
                     /locus_tag="ACHA5T_000529"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFM8886133.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(22224..23459)
                     /gene="fabF"
                     /locus_tag="ACHA5T_000530"
     CDS             complement(22224..23459)
                     /gene="fabF"
                     /locus_tag="ACHA5T_000530"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFM8886134.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(23478..24215)
                     /gene="fabG"
                     /locus_tag="ACHA5T_000531"
     CDS             complement(23478..24215)
                     /gene="fabG"
                     /locus_tag="ACHA5T_000531"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFM8886135.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(24227..25144)
                     /gene="fabD"
                     /locus_tag="ACHA5T_000532"
     CDS             complement(24227..25144)
                     /gene="fabD"
                     /locus_tag="ACHA5T_000532"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFM8886136.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(25251..25475)
                     /locus_tag="ACHA5T_000533"
     CDS             complement(25251..25475)
                     /locus_tag="ACHA5T_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFM8886137.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(25528..26490)
                     /locus_tag="ACHA5T_000534"
     CDS             complement(25528..26490)
                     /locus_tag="ACHA5T_000534"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFM8886138.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(26500..26940)
                     /locus_tag="ACHA5T_000535"
     CDS             complement(26500..26940)
                     /locus_tag="ACHA5T_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8886139.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            27246..28211
                     /locus_tag="ACHA5T_000536"
     CDS             27246..28211
                     /locus_tag="ACHA5T_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM8886140.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(28268..28498)
                     /locus_tag="ACHA5T_000537"
     CDS             complement(28268..28498)
                     /locus_tag="ACHA5T_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFM8886141.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(28584..29471)
                     /locus_tag="ACHA5T_000538"
     CDS             complement(28584..29471)
                     /locus_tag="ACHA5T_000538"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8886142.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(29560..30783)
                     /locus_tag="ACHA5T_000539"
     CDS             complement(29560..30783)
                     /locus_tag="ACHA5T_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM8886143.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(30800..31846)
                     /locus_tag="ACHA5T_000540"
     CDS             complement(30800..31846)
                     /locus_tag="ACHA5T_000540"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM8886144.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIRSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(32010..33737)
                     /gene="ptsP"
                     /locus_tag="ACHA5T_000541"
     CDS             complement(32010..33737)
                     /gene="ptsP"
                     /locus_tag="ACHA5T_000541"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFM8886145.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(33737..34003)
                     /locus_tag="ACHA5T_000542"
     CDS             complement(33737..34003)
                     /locus_tag="ACHA5T_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFM8886146.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            34482..36716
                     /locus_tag="ACHA5T_000543"
     CDS             34482..36716
                     /locus_tag="ACHA5T_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8886147.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            36864..37184
                     /locus_tag="ACHA5T_000544"
     CDS             36864..37184
                     /locus_tag="ACHA5T_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFM8886148.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
BASE COUNT        10509 a         8076 c         6510 g        12232 t
ORIGIN      
        1 tgagccagaa tgtggttcat tctgactcag atttgatttt gcacaactca tcagtcctta
       61 ttgacaaaga gccacaattt ttgtcacaac cttttttgcc gagtaaacgg tgttcaaaag
      121 ccagtttctc ggtatgaagt caaatttaag caaaaatgtg agaagctatt ttcacaaaat
      181 ttttcttcat actcgaaact aagcggcttt ttcacgacct cttttttatt ttgccatgaa
      241 catatcataa tacgcgtcca ttatttcttt agcaatcgtc aagtttatgt ggtcttgttc
      301 gtctaataag ttcggcaaga caacactgat ggcaatttcc ggattgtctg ttggtccgta
      361 tgccacgata ttactattga ctgtcgcaat atctggatga ccttccgcaa ccgtttctgc
      421 agtccctgtt tttgcggaaa ggtccatttt cgcactggct aatggtttgg ctgttgtgta
      481 aggatcggta ccatggacaa cttggtggaa tccttcacgg aggatcgcca tattttctgc
      541 agaaatattg accgtatttt ctactgttgt gccaatttct ttcttcactt cacccaagtt
      601 tccattttcg tcattgccat agatcgcttt gactaggtgc ggctgtactc gtttcccgcc
      661 attagcgact gttgaagcat attgagctaa ttggattggt gtatatgtgt caaactgtcc
      721 aaatgccaaa tcggtaaagt tacctggagc ataccattct ttgccattat ccgcattgaa
      781 cttcttcata taatcaacag aacgtgagat cccaggagat tcattcggca aatcgatccc
      841 agtcgttgta ccaagaccaa actctttgaa agagtctctc aattgctgat aagctttagc
      901 ttcttcatct aaactaggta gtcccattcc aggtgtatag tctagtccta acatctttaa
      961 cgccactttg atcatatacg tattagaaga caattccgat gctttgactg catcaagagc
     1021 aacttgtccg cttcgattaa agacagagct tttttctgaa gcaccttgca gacgaatcgg
     1081 ctcatcaatc aaaacttgat ttccgctgat cgcgttactt gcccagcctg ctgtcaaagt
     1141 accagccttg actacagacc ctggatcaaa ggcgctagta atcgtgccta aagcattctc
     1201 ggtgatttct ttggaacctt gttcatgact gaaaccagtc attgctagca cttctcctgt
     1261 ctgaggattc atagccactg cataagcacc aggcgaatac tgtgctttgc cgttgttgat
     1321 caatgtttga taattccttt tcaaaatttc ttctactttt gattggaatt ctgcattcat
     1381 agataacatt aaattagaac ctttttcccc agagaagatt tctttttgat tcgaaacatt
     1441 tccttcatta tcaagggaaa cttcatattg ggttttggtt ccttgcagga catcttcata
     1501 ttgtttttcc aaataacttt gtcctacacg gtcattccga gaataacctt ttgctaggta
     1561 tttctctgct tcatctgctg gaagtccttg cttttcagtc gtcacactgc caagaatact
     1621 tttcagtgaa cttgctgcat tatattctcg tgtccagtct gtgccagtcg aaactcctgg
     1681 aagttcagaa gcgcgttctg ccacaacagc taactcttga tcagtaacac cagagttttt
     1741 gatatagaca gtgtttaatt catatgcact attcatccgt ttaaagatgg tagcaatttt
     1801 taattgctcc tcgtttaaat taatctcatc agctgtgact ttattgacca tcgcactata
     1861 ttgttcgctg ttccctaatt gcttttcttt tgccgaaagt ctttttgtgg cttctttcaa
     1921 atgtttggga tcagccagcc aaaaatcttt tttatctcta tctgtcagat tttcatctac
     1981 cggaacatcg atcagcgagt tcagcttcgt tgcgatttca agcaaatctt tagccgtaac
     2041 ttgtgttcct ctcgtatatg tgatggcctg attcgcttta ttcgtaacta aagcttttcc
     2101 tgtggagtcg tagatcattc cgcgaggagt actttcttgg accgtaatcg tagaagtaga
     2161 cttcactcgc gcctccatct cttcgccgtt cacgatctgc aaataaccca atctagcaat
     2221 caacgcaaca aataacgtaa atataatgaa aaaaagaaaa tttaagcgaa aaggaacatg
     2281 ggaccggcga aacgttttgt tcttaggttc ttgtgcacct tttcttattt tttctaacca
     2341 agaagtattt aatatttttt tcataaaatc tttcatcatt tcctatcctt ccgcctattt
     2401 acattcatgc ttcattgtac ccaaaaactt cttaataaaa aagaaaaaat gggcggagca
     2461 agaaattctt tacctaattt caagataaaa tcaacttttt gtttcacagc aatcacttaa
     2521 agttacatat ccaactattt ttttcattaa aaaataaact tatttttgaa aattatgttt
     2581 tcttaagtcg tgaaacactt tgatggatca tttatttgtg gaaaactacg ttttgttatt
     2641 ctaaaaaact tatataaaaa atagtaaggt ttttatttcg aatccataaa atattcttgg
     2701 attgcttctc gtgttttttt cactttttcg ctaagctttt cgtttttagc catatagaaa
     2761 taaaaaatga tatcgatcgt agcaaattgg gccaaaagtg aattagttgc cgcacttcga
     2821 ttcgtgattt cttttggtct agatgtttgt acgagtacat cggctatctt gctaagcggg
     2881 ttatttccaa attgcgtcaa cgcaatgatt tcgatattca tgcttttcgc cagctcggct
     2941 aatgcgacta catctgcact tctacctgag tttgaaacaa gccaaagcac tttctttttc
     3001 tcattgctga caagttgcgg gagcaagacg tggatatctt tttcaaaaat gattggctta
     3061 ccgacacgag accatttttg cagaatatct tctactacta aagaagaggc gccaacccca
     3121 tataaaaaaa cagtctctgc cgattccaaa cactctacga ctttttggat cgtttcttct
     3181 tctagaatat ctactgtttc ttttatcgct acttgtgcat tgttaccaag tttgtttttg
     3241 atagagtaaa aggactcatt tgcttctata tcactaaaac cttcatcgat tccagtctgt
     3301 agttctgttg aaatcgtgat tttgaagtcc gtcagacttt gaaaaccgat ttttttggaa
     3361 aaacgtacaa ctgtcggcgc actcaacccg gaagcttctg ctaattcatt agctgtcatt
     3421 gtagggacct tctcaatatt accgataatg tagttggcca actttttttc tgctgaagaa
     3481 taatcttcca tcatactttc aatacgacta attacgctca taagttcctc caccctttta
     3541 atgatagtct atgtaagtat accatatcag acccttattc acggtcgtat actggataaa
     3601 aaacaaaaaa acaatccgcc caacagaata tcttatccca tcaatcccac agattctatt
     3661 gagcatctgt gtctttgagg aatacctgtt atctattgtt cggattgctt tctatctact
     3721 acttttttac aggaaattta cctattagtt ttctctgctg taatttttga cttctgcttc
     3781 catagcatca ataaagatat tcaaatcttc tacagctgtt tctttacttc cataacgacg
     3841 tacgttcact gttgcatctt ccaattcctt gtctccaaca actaattggt aagggatctt
     3901 ttgtgtttga gaggcacgta tcttatagcc cattttttca ttacgatcat caacttctac
     3961 acgcaacccc ttcatttgca aacgttcttt gatttcataa gcataatcgc catgtgcatc
     4021 aacagaaaca gggatgattg ttgcttgtat aggtgccaac catgttggaa aagcgccttt
     4081 gtatacttct gttaaataag cgacaaaacg ttccattgta gaaacaattc cgcggtggat
     4141 aactactgga cgatgcgtat tttccccgtc ttctccaaca tatgtcaaat caaagcgctc
     4201 tggcaataag aagtctaatt gtatagttga taatgtttct tctgtaccaa gagctgtttt
     4261 gacttgaaca tctagttttg gtccgtaaaa ggctgcctcg ccttctgctt caaagtaatc
     4321 tagttctaat tcatccatag cggctttcag cattgcttgg gctttttccc acattgcgtc
     4381 atcatcaaaa tatttatctg tattgtttgg atcgcgatag ctcaagcgga aacgataatc
     4441 tgtaatattg aagtcagcat atactgccac catcaattca agcgtacgtt taaattcatc
     4501 tttgatttga tcaggacgaa caaatgtatg accatcattc aacgtcattt ctcgtacacg
     4561 ttgtaaacct gacaaagcac ctgatttttc atagcgatgc atcattccta attcagcgat
     4621 acgaattggt aattcacgat aagaatggat cgtgtttttg tagaccatca tgtgatgagg
     4681 gcagttcatt gggcgaagaa caagcatttc gccatctccc atatccattg gcgggaacat
     4741 gtcttcttga taatgatccc agtgtccaga tgttttgtac aactcaacat ctcccattat
     4801 tggtgtatat acatgttggt aacctagact aacttctttg tctacgatat aacgttcgat
     4861 cgtgcgtcgg attgttgccc cttttggaag ccagaatggc aaaccagaac ctacttctgg
     4921 ggaaaccatg aacaagtcaa gttcttttcc tagtttacga tggtctcttt cttttgcttc
     4981 ttcacggaga cggatatatt ctttcaaagc tttcttgtca aaaaaggctg tcccgtatac
     5041 acgttgcatc atttgattgt ttgagtttcc acgccaataa gcacctgcca ctgataataa
     5101 tttaaatact tgtatacggc cagtggatgg aacatgtggt ccacgacaca aatcgatgaa
     5161 ttcgccttgt tgataagcag taatcacttc ttcttctggc aattcactaa tcagttctac
     5221 tttgtacgga tcacttgcaa aaatttctaa tgcttcctgt tttgaaagaa cacggcgttc
     5281 gatcggcaga ttttctttga cgattttcat catttctgct tcgattgccg gcaaatcttc
     5341 tgcagttact tggttttgtc cattatctgt atcgtagtaa aaccctgaat cgatagctgg
     5401 tccaacgcca aagtggatat ttggatagag gcgacgcatg gcttgggcca tcaaatgagc
     5461 tgctgaatga cggactaagc caagtgcatc ttcatggtct ggtgtgatga tctcaatact
     5521 tgcatcttcc tcgattggac gagtcaaatc aacgagtttt ccgttcactt taccagctaa
     5581 agcttttttt gctaagcttt ttgaaatgct ttcagcaatt gccaatgttg ttgttccaga
     5641 ctcaaattct ttgactgcac catctggaaa agtaattttt atcataatag tttcctcctt
     5701 gaaatgtttt gattgatcag atcatcaagc tgaaaaacaa aaaaatccta gcatctcttg
     5761 aaaagagata ctaggacgaa aagttcgtgg ttccacctaa tgttcaaacg caaatgcgtt
     5821 cctcaagcgt tgtaacggtc cgaccgccct tgcttcaaca agggtttcga aagtggtcat
     5881 cctttggttt tctcctggaa aagtttcagc ctagcttttc ctctcttatc gaagactctc
     5941 aaattcagtt ggctttctca tcaatcaatt atgtgtttat ttaaccacta ataaatagaa
     6001 aaatcaagtg ggaaactaaa cttatttttt ccattcccca tttacaccgt atgttccttt
     6061 tttcatctca ttgatgatga catggatgtg ttcttttggt gcctgcgtgt ttttatgaac
     6121 agcttccgtg atatctttca tcatattttc taattgttcc ggactgcgac cttctactaa
     6181 ttcgacatgt acgaatggca taatgctctc tcctctttct gatcgttctt ttttcaactc
     6241 gattctatca tactatgttt gtcttgggta ctgaatcagg aaaaatggct ggatcttttt
     6301 ttgctctcaa ggcactcaaa atagagaccg tatccacgac ttcttgcaaa acagctccga
     6361 ataatgcggg gattagtccc gtacttgcaa ttaacatcaa aaaaatacaa ataaagatgc
     6421 caatcagtac agactgtttc gctattctca tcgtatctct tgaaatcact actgcttttg
     6481 ctacacgcaa gagatcatct ttcaagatga ccgcatctgc actttcacta gcagctgtag
     6541 aaccatgagc ccccattgca ataccgatat ctgcagcagc aagtgctggt gcgtcattca
     6601 cgccgtctcc taccatcgca acaggtcttt gatctttagg gatactttct aaaatggtca
     6661 gtttatcttg aggcaagcta cgggcatgta cagaatctaa ttggactttt tctgcaatcg
     6721 tttgagcaat ggcagatcca tccccggtga tcatcatcaa atttttcaca tcaagttttc
     6781 ttagctcctt gattgtcttt gctgcttctg ggcgaatctg atccgaaaat aggatcgttc
     6841 ctgcaaaaga tcgatcaatt gttacataga ttgctgtatt ggacgtttct atttcttcag
     6901 ctgaaacaaa agaggcacgt ccaatcttta cttcatgatt attgactact gcttggattc
     6961 cttgaccggt cacttctttc agttgactaa caggatacag ttggctgttt ttcacgttgt
     7021 tcactaacga acgagctaag atatggctag attcctgttc tgcactagct gcatacagaa
     7081 gtagctcttc tttttttatt ccattgactg gctgaatcgc ctctacttcc aatatccctt
     7141 tggtaagcgt tcctgtttta tcaaaagcaa tcgtttttgt acgtgcgagc ttttcaatcg
     7201 ttgttccagt ttttacaacg atcccgtttt tacttgaacg gctcattcct gcaactaaag
     7261 caatgggcgc agctagaatc agtggacaag gtgatgccac taccagtacc tcagcaaatc
     7321 gtaaagggtc ttttgagaac caccaagaaa taccaccgat caaataagca atcaacgtga
     7381 aagggactgc ataacgatca gctaaacgga caaatcgggc aggcttttct tttgattctt
     7441 cgactagttt aataatcatt tgatactggc tgtctgcggc acgtttttca gcgatcattt
     7501 ggatcgatcc gtctccattt aaagaccctg acataatctg atctccaggt ttcttggttt
     7561 ccgggcggga ctcacctgtc aaagaagatt catcgaccga agattctcct ttggtcactc
     7621 gaccatctgc gggtacaagt tcgccaggct tgactagtaa ctggtcatca atatttattt
     7681 catcgattac tatatcctgc aattttcctt ctttgaatcg atgggcaatt cttggggtgt
     7741 tatctaacaa agactgcagt tctttacccg ctcgtttatt cgcgtaatcc tctagactat
     7801 ctccgcccgt aagcatgatc aatatcatca aactagccca gtattctttg acggctaatg
     7861 ttgcaaggat tgcggtaatc gctaatacat ctacgccata tcttcctgaa cgtaacgtct
     7921 tgatcatttc caaaaacatc aagaaggcga gaatccctcc agtaaatgcg acaatcgcaa
     7981 atgcaagggc cggctggtga aaaacaaatt cacttaataa agctatccca ccaataagca
     8041 aagtaagtaa aaattttttg aaatgtgtca ttgttttttc tcctctttta tgtgacttag
     8101 agaggttagt aataaatttc cagctgcctc tttcatctta agcatagtcc ttgactagca
     8161 ctttttaaaa caataggcct taattgaaaa caactatcac tatcagcgtt tccaaaattc
     8221 atcagttcta caaacaaaaa tattcagcaa ggattacttt ttcgattcat cagttaaaaa
     8281 gtaccttttt tcgaaaaact atgctatact tttaattcgt tatttacggc ggaaggagca
     8341 aacatgcgat gaatgagaaa caactagaac aaacgcacgt ggatgaaacg atcgaactaa
     8401 tccagatcga acaaaatatg ctggatacaa aacaacaaga attgactcag gaaatgaatc
     8461 attcgacaaa agacacagct aatcacaaga ttcgagctgg ttccaatgaa gctttttatg
     8521 aatctgctgt tgaatatcgc caacatgagc aggaattgct tttgaaatac catactcttg
     8581 aagcacagca aaaacgcctg caaacgttgt ctgtcatgaa agatagtcct tattttgccc
     8641 gtatcgactt taaagaagac cgtgaaacag aaacattata tctaggtatc gcttctttaa
     8701 gagatctggc agaagaaaca atcgttattg actggcgcgc accaattgcc aatctttatt
     8761 atgaaggtga gattggtccc gcattttatg aaactgaaac agatagaata gatgtcgatc
     8821 tgcagttgaa acgacaattt aaaatccaag aaggacgtat tttatctatg gtagatacgt
     8881 cggaagtaat caatgatgac tttttgctag agatattgga tgaatcatca agcaaccata
     8941 tgaaaaacat cgtctctacg atacaaaaag cacaaaatgc aatcattcgg gacacaacaa
     9001 gtaaagtaat gctgattgaa ggaatcgccg gaagtggaaa gacatccgct ttgcttcaac
     9061 gtattgcttt tctgttgtat cataatcgca agtggctcga ggctgaaaac gttcttctat
     9121 tttcacccaa ccatttgttt tctgattaca tctcgactgt attgccttca ttaggtgaaa
     9181 gcggcgtgcc tacacagaca ttcaaaaatt atttggtgca gctgctgcca gagttttctt
     9241 tactagatga acagcaacaa gaaagtggtt ttttgctagg ggaaaaagat ccgatacaag
     9301 tgatgaaaag cggattgacc ttagtcgatc agattgatcg ctatatccaa ggcatcacaa
     9361 actacggtcc cttgttccgt gatatgaaaa taaatggccg tacgatccta tcaaaggaat
     9421 cgattcgcca atggtataaa gaaacaaatg aacaattacc tttacatcat agactttctc
     9481 tattacaaac gaagctcctg aaaaaattag gcggtctgca aaaagacgaa acacgtcagc
     9541 aatgggtcaa agatctggcg gaagaacagc tccaagaact atacgcaaca gatccaaatc
     9601 ttgaatatac agaacaaaag gagcgttcat tacgtaaaaa actggccaac caaatcgtac
     9661 gtaaaagatt ccgtcgaatc catcgaggga tcatgaatta tcaatttgtc aatataacga
     9721 aacaatatct ccactttcta caaactgtgc cactttctct tctaacacga cataaaatca
     9781 gcgagaccag gtggcaggaa catttgacaa ctgttcgtac acacttgcgt caaagagaat
     9841 tgcgtcaaga ggatgctgta ctattctttt tactgatgcg ccgaacatat ccagtgacgg
     9901 ttacacacaa agctcgctat atctttattg atgaaatgca ggatttcgca ccatcacaag
     9961 tggcattgct gcaagccttg ttcccaaaag caaatttgac tttctgtgga gatctaaatc
    10021 aaaatgtctt tggaaatgaa acgatcgttg gatcattgga agaccttttc gtggaccgtg
    10081 aagtcactcg tttccaatta acgacaagct atcgctccac aaaagaaatc actgattttg
    10141 ctaatcattt tattgtgaaa gaacatcaag tagaaacaac agcaagaaaa ggagaccttc
    10201 ctttgctcat ccaaggaaca acagatgata tcaaattgaa ttggttagca gataccattc
    10261 gaacgactca gggaaaagcc cggtattggc gtacagcaat catagctaaa acacaagaag
    10321 aatgcgaagc tctttatgct caactgccag acgagttgaa acaacaagtc cagctgattt
    10381 taaatgagag tgactttatg aagcgttcca ttattttact tccagccttt ttagcaaaag
    10441 gtctggagtt tgaccgagtt ttcttgtggg atgtagatgc agaacatttt tcttctcagc
    10501 aagacaaatt gatcctttac accatgtgta cacgtgcgat gcatgaattg acccttttta
    10561 cctccaataa tgaaagtcct ttgttaaaca atatggatat ttcatcttat gagaaaaaag
    10621 tactgtaagt aaaaagacaa ctgctatcat taatgttagc agttgtcttt ttatttacaa
    10681 taataattgc agaaaaagta attcaatcat ttaaggtcat ttattctata tataaatcca
    10741 aatctgtttc aggatatttt aatttgacaa gcgaaataat cattttcagt tcttccatgt
    10801 tttctggata ttctgggtca aataccttgc tatgacggcc aatcccgcca ttgtcgatca
    10861 atcggtaaat tcgattccag tcaatgaatt ggtcaattga ttggaaattg ctgattggat
    10921 agccgccaca atccaaaatc tctgatacac gaaatgctag gtatgcacta tcgatcactt
    10981 tatttttttt cgtgtctatc ttctcatttc tttcgataag aattttttca ggacgatgtt
    11041 tcaagctcca ctctaatagt agaaagaaag cctccatccg atactcttct tcaaataacc
    11101 gaaaggctga atcagaacga cgaaaaagca gcggaatcag ataattttgt ctataaatca
    11161 gcattggtgc gcggtccacc atacactcta aataacggtt cagataccct gtaccgacat
    11221 agtttcctaa tcgatagcct tcaacgccga atcttgcgta tttttgcgct aattttgaac
    11281 aatcagttac gttcacagct aactcttcta ctttgcggta accgcttaag atacgttcaa
    11341 aaggttctaa agtgattttt gcttccatat tcttccccgt ccatcttcca catacttcat
    11401 atcatttcac atgatactta tgctctcatc ataccacagg ttgaattaga aaagaaaata
    11461 aatcacacaa aaaagcaaga caatctgtta gtgagattgt cttgcttttc gaaaaagact
    11521 gaatactttt cgttaaaact ggttgcttag tcttctaatg ccacattatg gaacacatgc
    11581 tgtacatctt ctagatcttc cagtgcatca atcatttttt caaatgtttc ttgatcttct
    11641 ggagatagtg ttacctcgtt ttgagggatc atttggatct cagcgattga aaaatcagtg
    11701 attccttttt cttttaatgc ttcttgtata ctatggaaat ccgctacttc accgtaaacg
    11761 atgatttgtc cttcttcttc cattacatcg cgaacatcga tgtctttttc cattaggtat
    11821 tcaaggatct cgtcagcgtc ttctcctgca aaagcaaaga ttgctgtatt gtcaaacatg
    11881 taagaaaccg ctccggaaac acccatgttc ccgccatttt tcccaaatgc tgcccgtacg
    11941 tctgctgctg ttcgattcac attgttcgtc aatgtatcaa caatcaccat tgagccgctt
    12001 ggtccaaatc cttcatagcg caattcagag tatgtttcat ctcctgcacc ttttgctttt
    12061 tcgattgctc ggtcaataat atgttttggt acattataag ttttagcgcg atcaatgacg
    12121 aaacgtagtt tttggtttgc atgcggatca ggatcacctg attttgctgc cgcgtagatt
    12181 tcaatcccaa attttgcata aatccggcta ttattagcat ctttagcagt tttctttgct
    12241 acgatatttg cccatttacg tcccatattt tcacttcgct ttctgatttg atagtttcat
    12301 aacgcaaaaa cgtcctgttc cattatactt gtgaatcacg attctgtgaa gctttttttc
    12361 tttcttcctt cattcttctc atttttagtc aattatctat actcggattg ggatttgccc
    12421 gaaattctgt tccggaatat tgcctcccaa ttgatagatt tgatcacttc taactaacag
    12481 ttccgcttct tgtgcctctt tttttccgat tcgggaataa atagaaatct cttttttttc
    12541 ttttaagaat cttcttccag catcggtaaa tccaagtagt cgaagataat ttttattttg
    12601 ttggacagga atctcttgct ttttgatatt cagcaagaca taacaagata atcgttggat
    12661 ccgtgtccat gtatatcttt ttgtttttac ctttgacaca aaatcctcaa aactatcagc
    12721 agttgcagct gctgttttca tgcgcgattc tattccttcg gtcatctgat agatatctgt
    12781 cagttccagc aaagtagaag ataacaaccg ataacgcaaa tacggccaat aagattccca
    12841 agtctgcagc gaacaagttg aaagatcttc ttctgtctga ggcggcacaa gtccagctat
    12901 tctttttcct tcttctaacg catggcgaat ggctgttgca ctggctatcc tctgatctgt
    12961 gatgattttt tcatgatagc ctgcactttt acgtcggatc ggatgtaatt ccatagggtg
    13021 gacatacttg gcattttcct ttgcataact taatcccaag atatgatttg gcgaagaaaa
    13081 atcaagcgta atatcgggat acagactgcg aaaaacctca gtcatttttt gcggataact
    13141 atacgtttga tcagacagtt cttgaaatgc ctcttctatc ctatcttgat tctcttggac
    13201 aaagcgtccg aaactgctgt aatcaaacga agtctcttct tctgttccaa aacacaaact
    13261 gtcacagtcc agtgtttgca agatttttac tgcaccttta gcaaaatagt cagctgactg
    13321 aaccgcccat tcgacgggaa gctccactac taaatccaca ccattttgta aagcttcatt
    13381 cgcgcgatgc catttatcga tgatagccgg ttcgcctctt tgcaaaaaat tcccgctcat
    13441 tacagcaatg acgacctctg ctcctgttat ttttcgtgcc atttccacat gatagcgatg
    13501 tccattgtga aaaggattgt actctacgat aatcccgcag cttttcatgc gcatcctcct
    13561 tcatagttat cgataaaaaa gaaatagaga caagtaaaaa aaggcaaaaa tcggaaaaaa
    13621 tacccgaact atataaacta ttctcgcaaa cacaatactt ctgcgacgaa aagctgcact
    13681 gcaagagcaa tgcctatatc gttcggatat ataatttatg ttgaagattt attcttttct
    13741 tgttaactcg agcaggcaaa accgctagtc tttgtatgct acaaagaacc agcgctttga
    13801 tttctctgat gctgcttcgt ctgtaaaatc agaaaatgct tcgactttcg aaaagccgct
    13861 gttttccagc attcttaaat aactttccaa tggataggta cgctcttgat gtagttcatc
    13921 ttcacgaata aatcgaccag acccttcttg ttcttcaacg aaaaaggtaa gaaaatgttc
    13981 gatgctgtgt ttctcgcttc ctggataact atcccacaaa aaagcaaaat tatccccttg
    14041 ataatgatag ctatattctg gaaaaacctc atctacttga taggtggagt gtacgtcaaa
    14101 taaaaatcgg ccattttctt ctaacgcttg atagacttca tcaaacactt gctgtacctc
    14161 ttgtctattt ttcatatagc acagcgaatc agaaaaacag gtaattgcct gatattgtcc
    14221 gatttctgat aaatcgagca tattgccttc cacaaattgg atcgatgcgt cttcttcata
    14281 tgctcgctgg ctggcaatca tcaacatctc ttctgaaagg tctaaccctg tcactttata
    14341 tccatctttt gcaaaggcaa ccgctaatgc tccagtaccg catgccagtt ccaaaatatt
    14401 ttttgtgtct tcttgtaaat gacgtttcga aaaagctaac cattgctgat aaagtgtttc
    14461 gtccatcact tcatcgtaaa tgaaagcaaa tgtttcataa gccatatgtt aatccaacca
    14521 ttcactaaga tcgactagcg gagcatctga ccatagtttt tccagattat agaaatttcg
    14581 ttctggcgca tggaaaacat gtacgatcac atctccaaga tcgatcaata cccattttcc
    14641 gccttctttt ccttcaatac gttttacttc atatttgttt tcttcttctt tatcaatgat
    14701 ttcttctgta attgcgttga tttgacgttc gctatttgct gaacagatca tgaaataatc
    14761 tgccaacaag gaaacatttc gaacgtctaa agcgataatg tcttctgccc gttttgaatc
    14821 tgctgcttca atcgcaattt ttaattgttc tttactatct atgatgattt cctcctaata
    14881 tgagcagctt atttttttgc tacccagtaa ttatatgttt cgattgtttt tggataaatc
    14941 tgttgttctt gcgcaagcaa atgggccaat gtatgttttg tttcaaatgc tacagcttcg
    15001 tctagatcag cccatgcaat ggctcgtgca tcttgtaccc caggaaaatc tcgacctggt
    15061 tcaatatagt ctgctacata aatgattttg tcaaggagac tcatcttagc agccccagtc
    15121 gtatgcaccc ggattgcatg aagaatctct gcatcggtga ttcctaattc ccgctctaca
    15181 aacgaagcgc caacaagacc atgccagatt gcattattat aattcaataa ctccggatca
    15241 aaaccgtctc ttacgattac cattttgaac tcctcatctg gtcgttcttt tgcataatca
    15301 tgtgtcaatg cagcaatact agctttttca ggagaagcac cgtatttttt ggcaagagcc
    15361 acggctgttt cttctactcc aagcacatgc ttgaagcgtt gttcgctcat tctcatctgt
    15421 attttttgca tcaggttttc tctagaataa gcagtatatt cattactata gtccatattc
    15481 atacaaccct tcattttgaa tataatcaat tactttatct ggaacaaggt agcggataga
    15541 acagccttct ttgatctttt gacgtatttt cgtggaactg atgtcgatct caggtacatc
    15601 tacccaaatt acaggatatg gagtatccgt tgtgtatcct ggtcgacgta tacccacaaa
    15661 gttgaccata gaagtcagct catcgatttt ataccatttt ggcaaatatt ctaccatatc
    15721 cccaccgata ataaagtaat aatctgtatc cggattgttt tgcgtcaatt ccttcatagt
    15781 atcataggtg taacttttcc caccacgagc taattcaatc gtttcaatct gcaaaaaagg
    15841 attgtcttcc accgctaatt ccaacatgtt gagtcgatgt tttgcatcaa tcgtctgttt
    15901 ctcatcaaca tgaggtggct gataagaagg catcaaaaat acgcggtcaa gacctaaatt
    15961 ccggccagct tgttcagcca tcactaaatg tgcaaggtga acgggattga atgttccgcc
    16021 caagatccct acttgctttt tcttctctaa gaattgaggc atttcttcag gaaataattg
    16081 tgaacgaaca aatgtcttag cttgcgtatt catcatggtc actccttata ttgcttttac
    16141 ttcttttgat agcctttgat acttttcttt tgtggaaggt ttgtataata caataacacg
    16201 tccaatgatt tggacgattt cgcaatctat ttctttttct aatacttgag ctgcttcttc
    16261 tgcaatctca tctgtatttt gaagtaacga tactttgatc agttctcttt tttctaaagc
    16321 ttcttcaatt tgaacgatca ttgctgaatt gatcccacct tttccgattt gaaaaatcgg
    16381 ctgaagatga tgcgctttgc tacgtaaaaa acgcttttgt tttcctctta aactcataat
    16441 tttccttctt ccttaaatca atgctttgcg tttcaataca tctacaccct ttggcgccca
    16501 gcccgcaatt actactggcg ctgttacagt gatccagcct agaccggcaa aaacgatgtc
    16561 tgttttttct ttcacagaaa actcaaaacg aacaagctcc ggaaatgcct cgacttcatc
    16621 aggacgagga ggctgtaata atccacctac atgtttttga taaaattcgt ctgctttttc
    16681 cagtttcgtt cgatgaatat ttacatcatt tgacacataa gcaataaatg aagcacgatt
    16741 gccgctgata aaatcaaatc ttgcaagtcc gcccaagaaa agtgtttgcc cttcattcag
    16801 ctgataaact ttaggtttga tttcttttaa cggtgcaatg atccgcaaat cttttttacc
    16861 taagtagtgc gccatttgat ggcgatggat aattcccggt gtatcaatca aaaaatgccc
    16921 gtcatctaaa ggaatctcga ttttatccaa ggtcgttcct ggaaactgcg aagttgtgat
    16981 tacatcttgt actcccgctg tatttttgat gatttgattg ataagcgtcg attttccgac
    17041 atttgttact ccgacgatat agacatcccg tccatcgcgg tattcttcga ttttttctaa
    17101 tagttccgtc atctcatgag atttcttagc actagttaat aatacatcta ctggacgaat
    17161 tcctgcttcg taagcacgtt ccttcatcca ttgtgttaat ttagtttttt tcaatgattt
    17221 aggtaatata tccactttgt tcccaactaa aagtactgga ttgtcaccga caaaacggtg
    17281 tagtcctggg attagtgaac cattgaagtc aaaaatatcc accacattga cgatcaatgc
    17341 atctttttgc cctaattcat tcagtaagcg caaaaagtcg tcatccgtca atgagacatc
    17401 ttgggtctca ttataatggc gtaatctgaa acaacgctga caatagactt ccccggaagc
    17461 taaacctttt tccaaagccg tttgcggcgt gtatcctaat tcaccgggtt tgtttgtttg
    17521 tataacggca ccacagccga tacattgtaa ttcttctgtc attcgattcc acctcgccag
    17581 cccatatcag ggtgttttgc taatagatag gccataattc ttttttccca aaaacgatta
    17641 aattgtgtct tccaactatc tgttgcgata atgggtttta caaggatgct acgtatccct
    17701 gcacgattgg ctccacgtat atcagtcata atctgatcgc ctaccattac tatctcatca
    17761 tctgctaaat ccaattgttt ctttgctatg ttgatccctc ttgctaaagg tttcattgct
    17821 cgtgcaacat acaaaagatc aaatttttca atcgctcgcg cgacacgttt tgaattattg
    17881 tttgaaacaa caacaacagg gattcctgca tttttcattt ctaaaatcca agtaagcaac
    17941 tcttctgttc catctggatt attccatgca atcaacgtat tgtctaaatc ggtcaatact
    18001 gctttgatgc ctagattctt taattgggca ggtgtaatct gatagatggc ttctaccatc
    18061 catgttggtt tgtaatttga aaacatgctg tctcctcatc tgcaaaaaat gaaggagcac
    18121 ataaagtact cctcaacccc gtattatact gcatatcagc aaaaaaaact acctctatac
    18181 tcgttcgtct ttcagaaaaa gcatttcttt acaaaatgac caaaaataaa gattgatcac
    18241 agaaaaattt agaaacaaaa aacaactaat attggcggaa cctcttgtat cgtttttcca
    18301 gtaatttatg ctgatctaat cgaacaagtt ccgtcaatct ttcaatcaac gcttttttca
    18361 taatgtgtgc gactttatat cgtggcaact gctcgccgtt gaacgtttca ggaataactc
    18421 gatcaacgat tcctaactcg cgtagttctg gcgcagtaat tttcatcaac tcagctgctt
    18481 ctgcggcgcg actaccgtct ttccacaaaa tcgacgcaaa tccttctgga gaaagcacag
    18541 cataaatcgt ttcttccagc atccagactt catccgctaa acctagagca agggcaccgc
    18601 cacttccacc ttcaccaatg atgatggcga tgatcggtac ctttaaatca gacatttcga
    18661 gcagatttct ggcaatagcc tctccttcgc cgcgctcttc tgctcctaca ccgcaatatg
    18721 cacctgctgt attgatcaag gtaatcaccg gtctattgaa cttttccgct tgtttcatca
    18781 gtcgtaaagc ttttcgatag ccttcaggat ggggagaacc aaaatttctg cggatatttt
    18841 cctgcagatc ccgtcctttt tgaataccga tcactgttac cggtttgcca tctaatcgag
    18901 cgattccacc aacaatcgct tcgtcatctg caaataaacg atcaccatgg cattcctcga
    18961 aagaatcgaa gatctcttca aaaaaatcca agcttgtcca tcgttcttta gctcttgcta
    19021 actgaacgac atcatgtgct gtcttatcca cgatctatcc atcctttctg tgtatgcaat
    19081 tcaagaagcg tatggatttt ctgtttcaat tccatgcggg gaacaatctg atcgacaaaa
    19141 ccatgagata ataagaattc agccttttgg aaatcttctg gaagttcctg cttgatcgtc
    19201 tgttcgatga cgcgtcttcc agcaaaaccg atcaagcttt ggggttctgc tagaataata
    19261 tcgccttcca ttgcaaaact ggcagttact cctccggtcg tcgggtccgt taaaacagtt
    19321 agataaaaaa gaccttcatt gctatgccgt ttgacagcag cagagatttt tgccatctgc
    19381 attagagaaa agattccttc ttgcatccgt gcgccacctg aagctgtgaa caagacgact
    19441 ggaagacttt cctttgttgc tcgttcgaat aatctggtga tcttttctcc gacaatcgtt
    19501 cccatacttc ccatgataaa attaggatcc atcactccaa taatgaaggg aatcccctga
    19561 atggttgctt taccagtgag gactgcttca tgaagtcctg ttttatcttg cattttttta
    19621 attttttccg tatattcggg aaatgctagc ggatctttgg ttacaagatc cgtatcccat
    19681 tcttcaaaac ttttttcatc aataatcaaa gctagacgct gccatgcacc gattcgaaaa
    19741 gcgtatccgc aatgagggca tactttttct tcaccgatgt ctttcgtata aaggatatgt
    19801 ttgcaatttg gacactttgc ccacatgtta tcaggaacag atggcgagtt agcaggtttg
    19861 ctctctctat taggattgat ccgaatatat ttctttttct taaacagagc catctatgcg
    19921 cctcctatct ccgtttctgg tgtccaattc ggtaaaaatt cttcttgtaa aaatgctgtg
    19981 ctgtaatcac ctgctatgac tgcaggatga ctaatcaaat ccatctgaaa ttctgcgttc
    20041 gtgatgattc cttctgtgat aaattcactt aaggctcgct gcatcttcat taatgcttca
    20101 aaacgcgtat ttccatggac gattattttt gcaatcattg aatcataata tggcgggatc
    20161 gtatatcccg ggtaaactgc actgtctact cgcagtccca tcccacctgc tggaagcagt
    20221 aagttttgga tcttccctgg agaaggagca aaatggaaag caggattttc tgcattaatc
    20281 cggcattcga ttgcgtgtcc ttggaatact acttcttctt gacacacatt caaaggctca
    20341 ccggctgcta tctccacctg tttttttact aaatcgattc ctgtgaccat ttctgttact
    20401 ggatgttcca cttggattcg agtattcatt tccatgaaat aaaaatcgcc ttcttcatcc
    20461 atcagaaact cgatcgttcc ggcattttca taattgactg ctttagctgc tcgaacagca
    20521 gcgtctccta gtgcttctct ttttgtttgt gagatcacta cagaaggaga ttcttccagt
    20581 actttttgat tgttgcgttg taaagaacaa tctcgctcgc ctaaatgaat gacatgacca
    20641 aatcgatcac ctaagatctg aacttctata tggcgagcag gataaatgat tttttccaaa
    20701 tacatatcat catttccaaa tgccgctgct gcttcttgct gagctgaaga aaaatgctgt
    20761 gggagctctt cttttgattg aactttccga atgcctttcc cgccaccacc tgcagctgct
    20821 ttcagcatca ctggataacc aattctgtct gctatttcca atgcttcttc tactgtagat
    20881 aatacaccag tacttcctgg aatgactggg acatttgctt cttgcatcaa ttgacgcgca
    20941 ttgatcttat ttcccattgc atcgatcgtt gcacttttag gaccaataaa tgtgatattg
    21001 cactcttcac acatggaggc aaatcggcta ttttcagata aaaaaccaaa tcctggatga
    21061 attgcttcgg cttttgtcac gattgcagcg ctcagtactt cctgaacatt cagatatgaa
    21121 tcaacggctt ttgccgggcc aatgcagatt gcttcatctg ctaactgtgt atgaagtgct
    21181 tcggcatctg cttctgaata tatcgctacg gtttggatcc ctagttcgcg gcatgcacga
    21241 atgatacgta cagcgatttc accgcggtta gctatcaata ctttagaaaa catatttcac
    21301 ctatccaatc ataaaggtta attccgcttc ggctactttt ttaccgtcta ctttagcgat
    21361 tcctttgcct attccggctg aagctttcac tttcaatatc tcaacttcaa ggtataaggt
    21421 atctcccggt gtaactttct tacggaattt tgctttgtcc aatccgccga aataagccgt
    21481 ctttcccttg aattgttcaa gagaaagtaa tgcaactgct cctgcttgag ccattgcttc
    21541 aacaatcaat acccctggca taacaggttc ttctggaaaa tgtccttgaa aaaagggctc
    21601 attgatcgtt acatttttct tagcaacgat ccgttctcct tcaaccattt cttccactcg
    21661 atcaatcaaa agcattggat aacgatgtgg aatgatttct tttatttctt gtatgttcat
    21721 tgttttccct ctttcctgct ttgcttattt ttgctgaatc ctaaagagtg gctgtccata
    21781 ttcgacaact gactcgtttt ctacaaggat ttctgtgatc actccgtcgg cctcactagt
    21841 tatctcattc atcagtttca tcgcttcaat aatgcagata acttctcctt tggcaacttg
    21901 atcacctact tttttgaaag ctggtttgtc gggcgctggt tgcagataaa cgattcccac
    21961 gattggcgaa acaacctcga ttccatcaaa tactgccgat tctttttctt tcattatttc
    22021 tggagcagtt gttgcttgtt caggtatgac attgactggc tccgaaactg gcaacgtttc
    22081 gtttgtttga accatattca tgcctcgact tgttttattt ttattcatat acaattcaaa
    22141 ttgaccttca cgcaaatcga attccgttag cgaggattga tcaaattgac tgactaattc
    22201 tctgatttca ttgatatcca aaattattcc tcccagcgtt tcatgcacag aaccgcatta
    22261 tgtccgccaa accctaatga attactcatt gcataagtaa aacttgcagg tttgctttcg
    22321 tttttgacaa ctgtcaagtc aatctcagga tcttgttttt ctacattgat cgtaggtggg
    22381 ataaattgat gttctaatgc cagcaaagtt gccaaggctt cgatcccgcc ggctgctccc
    22441 aataaatgtc cagtcatcga tttcgtgctg ctgacgtaag tatctctgaa attttcacct
    22501 aatgcgattt ggattgcttt tgcttctgcc tggtcattgg ctggtgtact tgttccgtga
    22561 gcattgatat atccgacttg ttccggctgt acaccagctt cttccatcgc aagcttcatt
    22621 gctttagatg caccagaacc atctggagta ggagctgtca tatggtaagc gtcacagttt
    22681 gcaccgtatc caacgatttc tccatagatt ttggcaccgc gagcttgtgc atgttctaaa
    22741 gattcaagaa ctagaacgcc tgctccttca cccatcacaa aaccattccg attttcgtca
    22801 aacggaatcg aaccacgatt aggatcagtt gattctgaga gtgctgttaa agctgcaaat
    22861 ccagcaatcc ccatctcaca aatcgttgct tctgttcctc cagcaagaat gacatcagaa
    22921 tagccatgtt tgatgttacg gaatgcttcc ccaattgaat tcgttccgct agcacaagct
    22981 gttacgatag ctgtacaaat accttttgca cctacacgca aagcaatatt tccagctgcc
    23041 atgttggcaa tcgccattgg tacgaacatt ggagaaactc gttttgcacc tttttcatgt
    23101 agacgaataa cttggttttc gattgttgga agaccaccga tcccagatcc gatgatgacg
    23161 ccaaaccggt cttgattcat tttttcttta tctaaaccgc tcatctccat tgcttctaaa
    23221 gcagcgtaga ttccatagat cgaaaacata tccattcgtt tgctatcttt tttaataaaa
    23281 tatttatcgt aagggaaatc tttgacttca cccgctaagg taatccctgt ttctgaagca
    23341 tcaaatttag tgatcggtgc gatgccattc gtcccctctt ttaagctatt caaaaagctg
    23401 tctgcgtcat ttccaatagg tgaagtgact ccatagccag taattaccac tcgattcatt
    23461 tgtttgctct ccttttctta tccgtgcatg accaatccgc catcgacatt gatgacttgt
    23521 ccggtgatat atggattttg tgctaagaaa actgctgttt gtgcaatatc ttctacttgt
    23581 ccgaaacgct gcatcggaat ttgcttttcc gcttgttcct tgactttgtc tgctaaaact
    23641 tccgtcatat cagttgtgat gaatcctggc gcaatggcat tacatgtaat gcctctagtt
    23701 gcagcttctc gtgccacaga ttttgttaat cctactacgc cagctttact agctgcataa
    23761 tttgcttgac caatatttcc aatcaaccca gatacactag agagattaat gatggcccct
    23821 tctcgctgct tcatcatttt tttcaatacg tgctgcgtca tattgaaggt tccaatcaaa
    23881 ttgatatcca gacatttttt gaaatcttct gcatccattc gcatcacaag tttgtcattt
    23941 gtgattccgg cattgtttac taacacatgg atcgatccaa gcttttcttc tgcttctttg
    24001 atcatttgtc ccgctttctc ataatcagag atgtcaccgg aaactcctac gcattttaca
    24061 ccgaatgctt cgatttcttc tatcttttct tttggaatct ctccgcgacc attcaaaatg
    24121 atatttgctc cagcttttgc aaaagctaag gccatcgctt tgccgattcc tcttgtacta
    24181 cctgtgataa atacattttt tccttttaca tccataacgt tcctccttat agtattgcta
    24241 ttgtttcatc aaatgtctgc ttattctcaa cacgcagaac tggtattgtt ttatcgattt
    24301 ttttcataaa tccgcttaat acttttccgg ggccgacttc gatgacttgt tcgatgccga
    24361 ttgtttttaa cttatggata ctctcataga aacgaacagg ttgcatgact tgctgttcaa
    24421 gtagtcccgc gattgtttct ttcttcatta tttccgttgt tgtgttgctt atgataggaa
    24481 aagaaggttc agagaattga atctgactta ggtccttcgc cagttttttt gctgccggtt
    24541 ctaaaatagc tgtatgaaaa ggcccgctga catttagagg gatcattctt ttgaaccctt
    24601 tttcttttaa tagtaaaaca gcttcgtcta ctgctttttc ttcgccacca atcacgatct
    24661 gctgaggtgt attataattt gcaggagata cgatgccgta cttgctagct tcatgacagc
    24721 tttcctcgat cgtttcaatc ggcgcattca tcactgcaac cattttcccg cttccagctg
    24781 gggcagcttc tgtcatataa gcgcctcttt ttgctactaa cgcaactgct tcagtgaagg
    24841 atagcgcatt actagctact aatgccgaat attctcctaa actcagtcct aaagctgcat
    24901 ctgggatgat tccatgctcc ttcaataagc gataatacgc gatgctgact gttagaatag
    24961 ctggctgtgt atattgcgtc tgatccaaac gttcattttc tgtaaagcaa agttcagcca
    25021 tatcgtagcc gagaatctca cttgcttcat caaatgtctg ctttacaacg gcttcttcat
    25081 ataaatcttt tcccatacct tgatattgtg caccttggcc actaaataag aatgctgttt
    25141 tcatgttttt ctccaatata tccagaatat atgtttgatt cttcctattc aaaaaggccg
    25201 agacattcct atttcaggaa atgtcccagc ccccaaccta attttaatga ttatgcagtt
    25261 tgtttttcta cgtaagtcac taaatcttga accgttttga ttccgtcttc tgtttcgatt
    25321 ttcacgtcaa attcatcttc aatttcattg atgatttgga ataaatccaa gctgtcagct
    25381 tctaagtctt cttggatgtc agtagtcaat ttgatttctt cagtttcttt tcctaattct
    25441 tctgcgatga tttcttgaat tttttcgaat accataataa tatattcctc cgatattgtt
    25501 tgctttttat ttttataatt taacagtcta caactccatg agaatacttc cccatgttaa
    25561 acctccgcca tagcctgtaa atattacttt ctgctgtttt cctaaggtga tctttccact
    25621 agaaactgct tcatccaata agatagggat gcttgcagca gatgtattcc catatttatc
    25681 catattggtc aggaactttt cttgcggcat ctttactttt cgggcaattt tctcaataat
    25741 tctgaggttt gcctgatgca aaagcagata atcaactggt ttgtctttca tgacttcttg
    25801 gatattgttt gcaacgtctc gtacggcaaa atcaaaaata tctctgccag tcatttgcaa
    25861 ataatacgaa ccttctgaat cttctgaata gaacggattt ttattttgct ggtagccagc
    25921 ggtcaatgag ctacttctgg taccgtcagc tgctaatttt tctttcagca attttttctg
    25981 tggattcgct tctaagagaa tcccacctgc tccatcgcca aacaacacag cagtcgtacg
    26041 atcagaccaa tcgattagct tagatagtgt ttcaccacca atcaccagtc ccttagttga
    26101 gccgcagcgg atcatttttt cagccatgct taacgcataa acaaatcctg agcacgcagc
    26161 actgatatca aaagcgatag catgagttgc tcccaatttc ccttgtactt gtgctgctac
    26221 agatggtgtc gtgtaatcgg gcgacattgt tgctactaaa ataaaatcca aatctttagg
    26281 gtttagattt ttctgtttta tcaatttttc cgccactaaa atgcataaat cagaagtctc
    26341 ctgatttgtt acacatcttc gttcacgaat acccgtcctt gacgaaatcc attcatcaga
    26401 ggtatccatc atcttggaaa gatcattatt agtaataatc ttttcaggaa ggacgctggc
    26461 tgttgcagta atcattccaa attgttccat taaatatcct cacttatagt cttgcaagaa
    26521 gtcgtgcaag tttttcagtg catccagcaa ggcatgctgt tcctccgtag ccattccgtc
    26581 taagatgttc ttgaccattt cacgatgaaa atgctgatgg acccgaaata gtaactttcc
    26641 ttttttcgtt agtcccaact ttacaacgcg gcggtcatct tcactgcgga tacgttcgac
    26701 atagcctttt tttactaact tgttaatagc agtggtcaag gtcccaacag taattgatag
    26761 ttctttggct acttctgaag ttgttttttt cttgtacatc ccgattgcct cgatcgtgtg
    26821 catctctgtg attgacagat ctttgaattg agattttttc agctcagact cttcgattgt
    26881 caaaatatca ttgaagacac taacaagata atcatttact gtttttaaat ctggttccat
    26941 gttccaccgt ccactaatta gtttgattat caaatctttt gatgttcaaa ctatatctta
    27001 acttttttca aaatgcaacc ttaaaagatc atttttttac tatatgagct ttttttaaga
    27061 ttttactcat ttaaaacaac tgataaatca gcatttataa tttcttttat tagaaaaatc
    27121 atctatctca catattttga ttatcaaagt attttctgtg aacagaacta tagaattccc
    27181 taaaacttgt ccattatttc aaaatttgtt atagtctttt tattcaaagc gtaaaaggag
    27241 atcctatgtt aaaagaaaaa atgaaaactc tccccatgct gccgggtgtc tatcttatga
    27301 aagaccgaga tggtaaggtc atctatgttg gtaaagcaaa acggctcaaa aatagagtaa
    27361 gcagctattt tcatcagaat aaacagcatt caaaaaaagt attacgtatg attcatcata
    27421 taacagattt tgatttcgtc gtagtagaca cggaattaga tgctttattg ctcgagtgtc
    27481 aattgatcca acattaccgt cctttttata accgacaaat gaattatttc agtaattata
    27541 attatgtaca tattaccaat aagggatttg tattaacaga tactcctacg gctcgaacat
    27601 acgggccttt tcgtctctat aaaaaaatgc cgtctatttt gcgtataatg gaagaaactt
    27661 atcaaatgcc ttggttgtct gaaattagtt tgttagcttt gcgagtccag cttcctgacc
    27721 tacaggaaat gacttttgaa cagaaaaaaa aggaactcca agggctgttt caaggccgaa
    27781 ataaaaagct actgacttac ttgaaaaaaa gacaacaaca tttcatctat cagttgaatt
    27841 tcgaaaaagc cggtatgctt caaaaagata tcgaattagt tacttatttt attagacgga
    27901 tccaggaaca aaagcagttt ttaagaacac catcgctcac cttcagcatg ccattagcag
    27961 cagatgagag tcagaaaaag cactacctcg tttgttatgg tcaacttgca gaaacaatga
    28021 ttacctctgg tgacgtttct cctgacttct attatgaaaa gaaagaagct catttatctt
    28081 tgaaacgtca attgtcaaaa gaagaaatcg atcctgtaca aatcttgatc agttaccgca
    28141 aaaaattaga aaaagaacag tttgaaattg aactgttaaa caaaaaagaa gctgaaaagc
    28201 aactaaatta gttgcttctc ggcttctttt aggcatcttc aacttcaaat gtcaattatc
    28261 ctaaaattta ttggattaag tcatagattt ccatcgctac gatatcgata ttatcaaatt
    28321 cgtattgctg atgagtatct gtttcagtta attcaaatgt atcggtcgct ttatcataag
    28381 ttactgtaca cttctctacg ccttcttttt caaaacggcg aacttgcagt tcattatctg
    28441 ctgactcgac catagcctcc agtcgtttta tgattgcgac taattgtgaa tgtgtcatcg
    28501 gaggtcctcc ttttcttatt tctgttatat tgtatcaaaa gcatagccta cttgcattga
    28561 ttttctctaa aaaaactcgt tttttatgat tttttttctg gtatttttcc ttcaggccac
    28621 caaagtttga tcaaagcttg ggttccgtca ttctcaaaac ctttatctgc caactcttca
    28681 tatagtttca atgcttgggt agttgctggc aatacaagat ccattttttc tgcttcttct
    28741 aatgcaatct ttaaatcttt gataaaatgt ttgacaaaaa aaccaggtgt gtaatcttct
    28801 tttaaaatac gaggagaata attgcttaaa gaccagttag ctgcacttcc acctgaaagc
    28861 gtctcaatca ctttctttaa atcaagtccg tttttttgtg catagacaag catttccgtc
    28921 attcctgtca tcgtacctgc aatcatgatc tgattagcca tttttgtatg ctgacctttc
    28981 ccagcagaac catgcaatgt aaatgtcgta ccaaattctt tgaataaagg cagtacttga
    29041 tcataaacgg cttcttctcc accgaccatg atcgtcagtg taccattctt tgctcctaag
    29101 tcaccaccgg ataccggtgc atctaacgca tctgcgcctt tttcttttgc tgtttttgca
    29161 attttttctg ccaggcttgg tgtacttgta gtcaaatcaa ctaaaatatg tcctgagata
    29221 tctgcagaaa aaatccccga ctgtccatag tagatttctt ctacatcttt ggggtaacct
    29281 accattgtaa agagaatatt actttgttct gctaccgctt ttggtgtatc tgcccagata
    29341 gctccttctg ttactaaagg atcagctttt gcttttgtcc gattatagac agctacttcg
    29401 tgtcctgctt ctagtagatg gcgtgcgaca gcacttccca tgacgcctgt tccgataaaa
    29461 ccaagtttca ttttttcgac ctcctaaaaa tcaaaaatcc ttgcttctat gctagtatac
    29521 gctagaaaca aggatttttt cataaaaaat gtttgaattt tatcttttaa atgaatttaa
    29581 ttttattttg attcgttcaa tcgagtcttc ttcttctttt cttcgcatct cttcttcatg
    29641 ataaacaacc atgtcttgat aaaattcgat catagcttcg ccgaaatgtg tagatgaaat
    29701 atcataaagt ttagcttcta aaacttttgg atctttagga acttcactgg aaatataatc
    29761 aataagtgct ggtgcaaagt cttcgtcggt ttcaaaagtt ttccctaatg aaggatgatc
    29821 gaacaatcgg ttcaaataag catttccttc cacgacacaa ggtgttccgg ctgccatcgc
    29881 ttctgtataa gtaagccctt gtgtttctga agtagaagca ctgacaaaat aatctgccgc
    29941 tttataataa agtgctacat cattgttgtc cacttctcct gtgaactgca caaattcttc
    30001 taactgcagt tctgagatca attcctctaa ctcttctaca taaggtcctt ttcctacgat
    30061 aactaatctg gcgttaggat aagcggagat gacctgagga aacccattaa tcaaggcttg
    30121 gatatttttc tcataagaaa tcctgcttaa agaaagaatc atcaaatggt tttcctgaag
    30181 acctagtgac tcccttaatt tgctgatatc ctgctgagtg atatcagaac gtttgaattt
    30241 ttcaatgtcg ataccagtag gaatcacgcg aagcggtgct attacaccat agctgcgtaa
    30301 tgtatcaata acacgttcac ttggacaaac tacacctgtc gtatgatttg caaataatct
    30361 agacaagtat tttacatggg attgcctgat cacttttcct ttaccgatat aatgaaggta
    30421 gtcttcatac atcgtatggt aagtatggat cagaggtatt ttcaacttct tagctaccat
    30481 cttcccaagt atccctgctc cgaattctgt atgggtatga ataagatcta gttctagttc
    30541 tttggcaacc aaataagcat accacatgcc tctaacaaca actcttcgat ccttgaaaga
    30601 aataaatggt acacttggca ttcgaatcac atctttttcc aacttatccg cattaggatc
    30661 ggtcgttgta aaaatataga cttcatgccc atgtctttct aattcttgtt ttaatgtttt
    30721 gatcgatgta gcaactccgc tgacttgcgg aaagtaggta tcggtaaaaa agccgatttt
    30781 catgtttttc gcctccaaac tagtccttga cctgatttac tttttttaat ttgctagaga
    30841 ctttttcatt cagtacactt tcgtatacat ttttaagttc ttcaccgatt ttattgatac
    30901 ttcgctcttt tgctgtttga taaccggctt gtgtcgtact cggtagttct tgattggtaa
    30961 tctgttggat cagtcggata aattcttcgt ttgtcttccc catatagcag ttcttgctat
    31021 cttctaacca gccattatac actggaatat ctcgaacaag tacgttttgt ttgcttgcta
    31081 aagcttctaa aacaacgatc ccttctgtct cttcataaga agggaaaaag aatagatctg
    31141 cacctgaata cgcaccttct atgatctctc cacgaatgta accaggaaac aggacatttt
    31201 caggatgatc ttcttttaca attttccgga tgttccgagg aatcgagtac atgggaacat
    31261 gaccaaacca aataaatgtg tattcgggca tcttcttagc aatttctacg aaatcaatga
    31321 ttcctttacg ttcgaaaaac aaaccgacac aaatgattac tttttgatcc ggagacagtt
    31381 tgaaatattc tctgaacttt tgttctttca ttggatcagg gtgatatttc tctaagtcga
    31441 tcccattgga aatagcttga atcggcacac ggataccata gctttccaac aactgttttg
    31501 aataaggtgt tggcgtgatc agatagtccg ctttttggta caatcctacc agatattttt
    31561 ttacgatagg agaaagctgg ttcgatctga taaaggaatt tcggaaatct tcctctgtag
    31621 aatgcgcatg atagacaact ttttttcctt ctcttctggc tttattgacc atattatgac
    31681 tattgattcc gtatgtattg atgtgcagga tgtcataatc ttctttttca tctagtgtat
    31741 aagagatacc gacttccgtc agagcacgtt tttgatgatc aagcgcacgc ccgattccag
    31801 attttgcaag catcttttca ctttcaaaat ataataatac cttcacctta cgtccccctt
    31861 cacttatata gtataacata gcctacaaac tgtcactcaa acttaagccc tatttttgtc
    31921 tattcttact gacaaaaacg ttttcttaaa aaagaacaaa aaaactggcg tagctctttt
    31981 acaagccaca ccagcttttg atacgttgtt tattttacat attcatgaac aagttcaacg
    32041 acttcttcca tcatatcgca ttctttcaat gcacgatcag caagttcagc cattttcgct
    32101 gtgtccaagc gtttcattaa actacgcgtt ttaagaatag atgtcgcact cattgagaac
    32161 tcatctagac ccatacctac tagcaatgga acagctgttt gatctccagc catttctcca
    32221 cacatacctg cccattttcc ttcagcgtgt gctgcatcaa ttacattttt gatcaaacgt
    32281 aaaattgatg ggttatatgg ttggtacaag taagaaacgc gttcgttcat acggtctgct
    32341 gccattgtgt attgaatcaa atcgttcgta cctacagaga agaaatctac ttcttttgca
    32401 aacttgtctg ctaagactgc tgctgctgga atctcgatca tgatgccgac ttggatcgta
    32461 tcagatactt ccttgccttc acttatcaat ttttgttttt cttcttcaaa gatcgctttt
    32521 gctgcgcgga attctttgag tgtagctacc atagggaaca tgatacgtaa attaccatga
    32581 acagatgcac gaagcaatgc acgcatttgt gtacggaaca tttcgtcgcc ttgttcagat
    32641 aaactgatac gcaatgcacg atatcctaag aatggattca tttcatgagg taattgcaga
    32701 tatggtagtt ctttatctcc accgatgtcc attgtacgaa caactacagg ttttccttcc
    32761 ataccttcta atacagcttt gtaagcttca tattggtcat cttctgttgg gaagtctgga
    32821 gaatccatat acaggaattc agtacggtac agaccaactg cttctccacc attattatgg
    32881 acaccaacta gatcttttgg tgttccgatg tttgcagcta attcaaaatg cttgccgtca
    32941 gctgtgacag tttctgcatt ttttaatttt tcccattctg ctttttgtgc tgcgtaatct
    33001 gcacctgctt tttcaaattc agctttttgt tcatctgtag gatcgatgat tacatcgcct
    33061 tcgatcccgt tgacagccaa aatatcgcct tctttgactt tagcagtaat ttcttttgtc
    33121 ccaacgattg ctggaatttc taaagaacgt gccatgatag cagaatgtga agtacgtcca
    33181 ccaatgtctg ttacaaacgc ttttacaaaa ttgcgatcta attgtgctgt atcacttgga
    33241 gtcaagtcat gtgcaacaac gaccacttct tcgttgatca ttgatggatt tggtaaagta
    33301 acgcctaata agtgcgctaa aatacgttta gcaacgtcac gaatatctgc agcacgctct
    33361 tgcatataag cattatcttc cattgcttcg aacataccga tatacatatc cgttacttct
    33421 tttagcgctg attcagcatt tacgctgttg tctttaatat tttgcttgat ttgacctacc
    33481 atttctggat ctgaaagaac catcaaatgg gcatcaaata cttgcgcttc tgcttcacct
    33541 aagctttgcg ctgctttttc acgaatttgc tgcaattctt cagtagattt agctaatgca
    33601 gcatccaaac gagcttcttc agccgcagta tcttctactg ttgccttact gaatgttaaa
    33661 tccggttgaa ctagcaggta agcttttgca acggctacac cgtcactagc ggcaatccct
    33721 tttagcattt caaccattat tcagataagc cttctttctt cattgtttca acgattgctg
    33781 ccattgcatc agcttcgtca gcaccttctg cagtaatagt aacatcagaa ccttgaccta
    33841 cacctaaaga cataacgccc atgattgatt tcaagtttac tgatttacct ttatactcta
    33901 agtttacatc agagttaaat ttgcttgctg tttgtactaa tagtgtagct ggacgagcgt
    33961 ggatcccagt ttctgctact acgtgaaatt ctttcttttc catattaatc ggtctccttt
    34021 aagtgagtgt tgtttttatg tgagggtttt cttttttagt aattcagaga atcctaacaa
    34081 atgccaccct ttcatctgta aagattaaca ttttttttcg ttagatacaa cagttttcat
    34141 tcaatttcag ataaaagtat tcaaattatt ccggattctt tttcatcctt ttcttcacat
    34201 tttataacaa acgtgtttat ttcctttttc ttgtcgcttt ctcttctcct gacgtttatc
    34261 attctttcct ttgaccctct ttgttttcat atgaaacgaa ttacttgctt ccctttttat
    34321 ttttagtata ataagagtca aaggtcagga atggtcaata gagatttcac cttttctacc
    34381 tcataaatct cttaaaaaac taggggttat tcttttacct catgaaaaac gagatgtaaa
    34441 atatggatgt acccattttt gtgaaaggac gtgaatgatt catgctttgt caaaactgcg
    34501 gcaagaatga ggcaacgatt cacttgtatg ccactgtcaa tggtcagcgg acacaactcg
    34561 attactgtca gagctgttat caaaaaatca aaaatcaaca aaatggaggt ctgactaata
    34621 tggcccaaaa tgatccattt ggatttggca gtttggacga tcttttccgt tccatgtctc
    34681 gccaaatgca acagcaagga aattatgagc aaacaccacc aacacaattt ggcggaaaca
    34741 acaacttcaa tggtggtcag ccacctcaag gcaatacaga aggtttattg ggagaatacg
    34801 gaatcaatat cacagaacaa gcacgacaag gtgatatcga tcctgtgatc ggacgcgatg
    34861 acgaaatcaa acgagtcatt gaaattttaa atcgccgaac caagaacaat cctgtcttga
    34921 ttggagaacc aggtgttggt aaaacagcag ttgttgaagg tttagcacaa aaaatcgtag
    34981 atggtgatgt accgcaaaaa ttaatggaca aagaagtcat tcgtttagat gtcgtatctt
    35041 tagtccaagg tactggtata cgaggacaat ttgaagaacg tatgcaaaaa ttgattgaag
    35101 agatccgtca agcagaaaat gtgatcctat tcatagatga agttcacgaa atcgtcggtg
    35161 ctggttctgc tggtgatgga aatatggatg ctggtaatat tttaaaacca gctttagctc
    35221 gaggagaatt gcaaatggtt ggtgctacta ccttaaatga gtaccgcatc atcgaaaaag
    35281 acgctgcttt agaacgtcgt atgcaacctg tacgggtaga tgaaccaact gtcgaagaaa
    35341 cgatcagtat tttgaaaggt ttgcaaaaac gttatgaaga ctatcatcat gtaaaatata
    35401 ctgatgaggc catcgaagca gccgcaactt tatccaatcg ttacgtccaa gatcgcttct
    35461 tgccggataa agccatcgat ttgctagatg aaacaggttc taagaaaaac ttgacgatcc
    35521 aaatcgttga tcctaaaaca atcgagaaaa aactgcaaga agcagaagaa caaaaagttt
    35581 tagcttctcg agaagaagat ttcgaaaaag cagcttatta tcgcgatcag atcaataaac
    35641 ttcaaaaaat gaaagaacgt cagctcactg aagaggaaac acctgtgatt accgaaaaag
    35701 atatggaaaa aatcgttgaa caacggacag gtattccagt gggtgaatta aaagaaaaag
    35761 aacagacaca actgaaaaac ttagcagatg acttgaaagc tcatgtcatc ggacaagata
    35821 acgctgtaga tcgagtagcg aaagcgattc gccgaaaccg tgttggatta aacaaacaga
    35881 atcgtccaat cggctccttc ttatttgttg gaccaactgg tgtaggtaaa acagagcttg
    35941 cgaaacaact tgcctacgaa ctattcggtt cacaagattc aatgattcgt ttcgatatgt
    36001 ctgaatatat ggaaaaacat agtgtctcta aattgattgg ttctcctcca ggttatgtcg
    36061 gatacgaaga agctggtcaa ttaacagaaa aagtacggag aaatccttac agtcttgtct
    36121 tgttagatga agtagaaaaa gctcatccag atgttttgca tatgttcttg caaatcttag
    36181 atgatggtcg attgacagat gcacaaggaa gaacggtcag ctttaaagat acgatcatca
    36241 ttatgacaag taatgccgga actggtaaag tagaagcaaa cgttggtttt ggtgcggcac
    36301 gcgaaggtgt tactcgttct gtattgaacc aattaaacaa ctactttaca ccagaatttc
    36361 tgaaccgttt tgacgggatc atcgaattca gtgcattatc aaaagaaaac ttgatgacaa
    36421 tcgtcacact aatgctagac gatgtcaacc aaatgctggc agcacagcaa cttcacatcg
    36481 aagttcctac gaatgtcaaa gaaaaattag ttgacttagg ctatgatcca tctatgggtg
    36541 ctcgtcctct tcgacgcacg atccaagaac aaatcgaaga tggtatcgct gaattttatc
    36601 ttgatcaccc ttctatccat gagttgaaag caaaactgga taaagatgga aaaatcatcg
    36661 ttacttctaa accagaacgt ttggctaaag aatcagccga agaaacggct gaataaatcc
    36721 agttttatag cgtgagacgg tatttctttg agaaatgccg tctcattctt ttttcagaaa
    36781 agtcaaaata ctgttcattt tataaaaaag aaattataat aagagaaaca agcaattaaa
    36841 aatacataaa agaggtgagc gccatgaaat tggtaaatgt aacaaacagc cattcccgtc
    36901 ttgtattaaa ccagcttgaa aacactgatg cccatctagt caaggtctac acagccggaa
    36961 ataccactat tgtctacact gaagcccctg aacataatga aattctgctg atcaatgaca
    37021 aacgtaaaat ccagccgaaa gaaatcgaag atgctaaaaa ttatttctta gataaaatca
    37081 aagacgccgt ttaccacgag gatgaaatca aagtaatcga attggcaggc cttgttgaaa
    37141 tctcgattcc aaaacatgaa ttaggcgcta cattagccgt gtaaacaaat tttgaacata
    37201 ataattaaag agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta
    37261 taaacggcga aagcagaagc aactccttcg gaaataagct gaaattcacg aaaattgctt
    37321 ttcaaat
//
LOCUS       DAYYGO010000012        35583 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000012 DAYYGO010000000
VERSION     DAYYGO010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.012"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            264..2030
                     /locus_tag="ACHA5T_000545"
     CDS             264..2030
                     /locus_tag="ACHA5T_000545"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFM8886149.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2132..3391
                     /locus_tag="ACHA5T_000546"
     CDS             2132..3391
                     /locus_tag="ACHA5T_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8886150.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3538..4839
                     /locus_tag="ACHA5T_000547"
     CDS             3538..4839
                     /locus_tag="ACHA5T_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8886151.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4842..5693
                     /locus_tag="ACHA5T_000548"
     CDS             4842..5693
                     /locus_tag="ACHA5T_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFM8886152.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5706..6521
                     /locus_tag="ACHA5T_000549"
     CDS             5706..6521
                     /locus_tag="ACHA5T_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFM8886153.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6672..7838
                     /locus_tag="ACHA5T_000550"
     CDS             6672..7838
                     /locus_tag="ACHA5T_000550"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8886154.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            8068..8715
                     /locus_tag="ACHA5T_000551"
     CDS             8068..8715
                     /locus_tag="ACHA5T_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFM8886155.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            8799..9743
                     /locus_tag="ACHA5T_000552"
     CDS             8799..9743
                     /locus_tag="ACHA5T_000552"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFM8886156.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            9878..11329
                     /locus_tag="ACHA5T_000553"
     CDS             9878..11329
                     /locus_tag="ACHA5T_000553"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFM8886157.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            11350..12186
                     /locus_tag="ACHA5T_000554"
     CDS             11350..12186
                     /locus_tag="ACHA5T_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFM8886158.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            12186..12845
                     /locus_tag="ACHA5T_000555"
     CDS             12186..12845
                     /locus_tag="ACHA5T_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8886159.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            12971..13660
                     /locus_tag="ACHA5T_000556"
     CDS             12971..13660
                     /locus_tag="ACHA5T_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886160.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            13666..14622
                     /locus_tag="ACHA5T_000557"
     CDS             13666..14622
                     /locus_tag="ACHA5T_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFM8886161.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            14666..15178
                     /locus_tag="ACHA5T_000558"
     CDS             14666..15178
                     /locus_tag="ACHA5T_000558"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8886162.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(15434..15982)
                     /locus_tag="ACHA5T_000559"
     CDS             complement(15434..15982)
                     /locus_tag="ACHA5T_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFM8886163.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(16071..17252)
                     /locus_tag="ACHA5T_000560"
     CDS             complement(16071..17252)
                     /locus_tag="ACHA5T_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFM8886164.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGLGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(17488..18996)
                     /locus_tag="ACHA5T_000561"
     CDS             complement(17488..18996)
                     /locus_tag="ACHA5T_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFM8886165.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            19155..19346
                     /locus_tag="ACHA5T_000562"
     CDS             19155..19346
                     /locus_tag="ACHA5T_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886166.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            19549..20466
                     /locus_tag="ACHA5T_000563"
     CDS             19549..20466
                     /locus_tag="ACHA5T_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFM8886167.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            20459..21532
                     /locus_tag="ACHA5T_000564"
     CDS             20459..21532
                     /locus_tag="ACHA5T_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886168.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            21680..22198
                     /locus_tag="ACHA5T_000565"
     CDS             21680..22198
                     /locus_tag="ACHA5T_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886169.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            22179..22571
                     /locus_tag="ACHA5T_000566"
     CDS             22179..22571
                     /locus_tag="ACHA5T_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFM8886170.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            22647..23582
                     /locus_tag="ACHA5T_000567"
     CDS             22647..23582
                     /locus_tag="ACHA5T_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM8886171.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            23715..24494
                     /locus_tag="ACHA5T_000568"
     CDS             23715..24494
                     /locus_tag="ACHA5T_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFM8886172.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            24487..24804
                     /locus_tag="ACHA5T_000569"
     CDS             24487..24804
                     /locus_tag="ACHA5T_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFM8886173.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            25048..26658
                     /locus_tag="ACHA5T_000570"
     CDS             25048..26658
                     /locus_tag="ACHA5T_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM8886174.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            26815..27663
                     /locus_tag="ACHA5T_000571"
     CDS             26815..27663
                     /locus_tag="ACHA5T_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8886175.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            27894..28373
                     /locus_tag="ACHA5T_000572"
     CDS             27894..28373
                     /locus_tag="ACHA5T_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFM8886176.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            28406..30634
                     /locus_tag="ACHA5T_000573"
     CDS             28406..30634
                     /locus_tag="ACHA5T_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8886177.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            30763..32406
                     /locus_tag="ACHA5T_000574"
     CDS             30763..32406
                     /locus_tag="ACHA5T_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFM8886178.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(32479..33024)
                     /locus_tag="ACHA5T_000575"
     CDS             complement(32479..33024)
                     /locus_tag="ACHA5T_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFM8886179.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(33047..33532)
                     /locus_tag="ACHA5T_000576"
     CDS             complement(33047..33532)
                     /locus_tag="ACHA5T_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8886180.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(33522..34334)
                     /locus_tag="ACHA5T_000577"
     CDS             complement(33522..34334)
                     /locus_tag="ACHA5T_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFM8886181.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(34473..>35583)
                     /locus_tag="ACHA5T_000578"
     CDS             complement(34473..>35583)
                     /locus_tag="ACHA5T_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFM8886182.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT        11645 a         6402 c         7112 g        10424 t
ORIGIN      
        1 tatacttcta cgccaaaaac tgttaaaaaa cttttacttt agaaaattgg agaaattgat
       61 cgcttaagat aaattcagga cactaaatag tacaggaagt agactaaaac ttttaaaagt
      121 tttaagtcta tctatttggt aagtttaaaa aataaaaatt cggtatcaaa aaactgttac
      181 cgataacatt tttgaaagcg ttgtcttttg cttctctatc acttatagtg atgacgtcat
      241 attaaaacac aaggaggaaa tcaatggata cagctgcaat ttatcatcga ccggaaagtg
      301 aatatgcatt tctatatacg aatacaagat ttcgtattcg gcttcgtact aggaaaaatg
      361 atatcaaaaa agtatatgtg ctatgtggag atccatatac cataacaact gaaaaatggt
      421 atcaaaaaca acgaccaatg aaaaaatgtc taagtaccaa tgttcatgat tattgggaaa
      481 ttgaagtgac gagcgaaaca agtcgcttac agtatgcgtt ccatgttgtt ggggaagata
      541 acacggactg tttttacggt gatcaaggaa tttttcctta tcgagaaaat gtgattactg
      601 aaccaaattt ttattttcga ataccttatt ttcatcaaat cgatcgattc actattccag
      661 aatgggtaaa agaaacagta tggtatcaaa tttttcccga aagatttgca aacggggaca
      721 aagcaaatga ccccgaacac actttacctt ggggaagtaa agatccagac cgagaagact
      781 ttttcggggg agatttgcaa ggtgtgatgg atcatttgga ttacttgacg gaccttggca
      841 tcaatggcat ctatttttgt cccatattta aagccacatc caatcataaa tacgatacga
      901 ttgattatta tgaggtggat cccgcttttg gcgataaaaa acttttaaaa aacttgattg
      961 atgaagcaca taaaagaggg atacgtatca tgttcgatgc tgtattcaat catatgggtg
     1021 tccattcacc acagtggcaa gacgttctca aaaacggaga aaaatcgatc tataaagact
     1081 ggttccacat ccgttttttc ccagtagatt cttatcagat gactgactta ccagaaactg
     1141 ctgaaaatat cccatatgat acttttgctt ttactccatt catgccgaaa ctaaatacag
     1201 caaatccaga agtgcaaaaa tatttgctgg atatcgcaac ttattggata aaagaattcg
     1261 atattgatgg atggcgtctt gatgttgcta atgaagtcga tcatcatttt tggaaaaaat
     1321 tcagagaagc agttacagaa atcaaaccag acatctatat ccttggagag atttggcatt
     1381 cctctcaagc ttggctgcaa ggggatgaat tccatgcagt gatgaattat gcatttacag
     1441 attcgattaa agattatttt gcaaaaaaga aaatcacagc ttcacaaatg gtcagtggaa
     1501 tgaatcatca gcaaatgctt tacagagatc aggtgaacga aggaacattc aacttattag
     1561 attctcatga tactgccagg atcttgaccc tttgccaagg taacaaagaa ttgatgaagt
     1621 ctgtgatggc ctttatgttt cttcaaaaag ggtctccttg tatctattac gggacagaaa
     1681 tagggatgac tggtgaagat gacccggatt gccgcaaatg tatgatatgg gagaaggagg
     1741 aacaagatct agagctattc ggttttataa aagaacttgt ttctctaagg aaacagttgt
     1801 ctaagattat ttctgaaggt tctactcagt ggctaatagt aaatgatcgt gaagataagc
     1861 tctatttcac acggaaacta gagggacaga tcatctatgt ttactttaat cagtcaaaag
     1921 aaccttgggt agtagaacag gaaaatgagg tgattcttag tcaaaattgt cagttgttag
     1981 aagacgggaa agcagagata cgacaatacg gatttttgat catgtgttga aaactgttac
     2041 cggtaacaac aagaaagcgt ttgcaaatga agatttgtgc ccattataat aagggtgtaa
     2101 cagcacgttg aatacaaagg aggaaatgaa gatgaaaaaa agaagtgtga aacagttggg
     2161 tttagggtta atctctttag gaatcttagg cggattagcg gcttgtggca atggtaactc
     2221 ttcagcagaa ggggaagccg atgaaggaaa aacgttaaca gtttccgttg attctggata
     2281 taaagattat ataaacgaaa taaaaaacac tttcgaaaaa gaaaacgatg tgaaaatcaa
     2341 gctagtagaa aaggacatgt tcgatcagtt ggaatcatta gcgttagatg ggcctgctgg
     2401 aaagggacct gacgttatga tggcagcata tgaccggatc ggtgcgttag gacagcaagg
     2461 acatctggcg gaagtaaaac tgggaaatga agcagcttac gatgaaacag acaaagccca
     2521 agtgacttat gatggaaaaa tctacggaga acctgcagta atcgagaccc ttgttttgta
     2581 ctataacaaa gatttagtcg atacagcacc atcaacgttt aaagatttgg agaatctatc
     2641 caaagacagt cgttttgctt ttgagtcaga agcaggaaaa aatacaggat ttttagctaa
     2701 atggactgac ttttattatt cctatggttt aattgcaggt tacggtggct atgtatttgg
     2761 tgatgatggc acaaatcctt cagatatcgg tttgaataat gcaggtgctg tagaaggaat
     2821 ctcttatgca acagattggt tcaaaaatgt ttggccaaag ggaatgcaag acataaaaag
     2881 tgcaggagac ttcgccagcc agcaattcat gtccaataaa actgcagcaa tcattgacgg
     2941 tccatggcaa gcgcaaacat acaaagaaaa caatatcaat tatggtgtag ccaaaattcc
     3001 tactctaaat aatggacagc cttatcaacc gtttggcgga ggaaaaggat gggtagtcag
     3061 caattatgct aaaaacaaag acctctctca aaaatggctt gattatgtaa cgaatcagga
     3121 aaatcaagaa aaattctttg aaatggtaaa tgaaatccct gcaaaccaac aagctaggga
     3181 aacagcaaag gctaaaaacg atgaattaac aacagctgtg attgaacaat atgaaacagc
     3241 tcaagcgatg ccaaacatcc cagaaatggg cgaagtttgg acaggtgcag aaaacttgat
     3301 gttcgatgca gcatcaggtt caaaaacacc aaaagaatcc gcagatgaag cagtaaaaac
     3361 aatcagtgaa gccatagaac aaaaatactg attaaaatga taaccgtcca tttttataaa
     3421 aaatggaaca ggtgcttcag ataaaagtgg caagagcgag atggaaaatt atcttgctct
     3481 tgttgccatt atcttaaaag cagtttgcgg tttagcaagt ttagaggggg cattcagatg
     3541 cagcaagata aaacaagcaa tatcaacgaa aaaaaaatca cgctttgttc acttattcca
     3601 gggatggggc agtttgtaaa tggccaagct tttaaaggta tcgttttttt cgccatattc
     3661 gtcctattca ttggtcagat gcttctgggc ggatttcatg cattggtagg attggttact
     3721 ttgggaagta taccaatgga agatcattca ttatttattt tgattgaagg cactttgcaa
     3781 ttcatcatta caatcatttt cttgattttt tacggtttga atcttttaga cgcttatcga
     3841 gtagcgaaga tgcgtcaaga gggcaaaaaa atcaatcgga cgatgaagga agtcatacat
     3901 aatataggtg atgaaggttt tccttatcta ttgacacttc ccgcttatct tctcatgatt
     3961 tttacgatca ttttcccagt cctagttaca ttgttcactg catttactaa ttatgacttc
     4021 aatcatattc caccagccag tttaattgat tgggtagggg ctaaaaactt ttttagtatc
     4081 ttctttttaa gctcatatcg taatactttt tttgcagtct tttcgtggac cgtgatttgg
     4141 actttatgtg caacaacttt gcaaatcacc cttggtattc tcacagcggt tgttgctcac
     4201 cagaaattta ttaaattcaa acgtattttc ggtatcattt tcttattacc atgggcggtc
     4261 ccagctttta ttactatcat gagtttttcg aatatattca acgatagcgt aggtgccatc
     4321 aatactcaag tgattccttt cataaaccag cttccttttg tagatatcag tgcaatctct
     4381 tggaaaacgg acccgaactg gacgaaagta gcaattatct tagtgcaggg ctggttagga
     4441 tttccctata tctatgtgat ggttacagga atcttacaat cgatttcaga agacttatat
     4501 gaagcagcaa aaatcgacgg agcaaatgca tggcagcgct tttcaaacat cacgcttcct
     4561 gctgtcttcc tagtggcagc gccaacattt attacacagt atacggggaa tttcaataat
     4621 ttctccatga tctatctgtt taacaatggc ggtcccggaa gcgtaggcgg aggagctggt
     4681 tctactgata ttcttatttc ctggatctat aaacttacga ctggaacttc tccgcagtat
     4741 tctatggctt cagcaatcac gttgattatc tccgtcatcg ttatctgtgt gtcattgctg
     4801 gtatttaaaa agacaaatgc gttcaatatg ggggattaac aatgaaaaaa tttcattcga
     4861 gtgctcgatt ccaaaaacga ctttctcact tttttactta cttttttctt ttaatactgt
     4921 cagtcattat catctatccg cttttgatca cagcaagtac tgccttcaag tcaggaaata
     4981 ttgcagcttt tagtttagat ctttcaagtg actggacttt aggaaacttt actcggctat
     5041 tcagtgaaac actttacggg acttggtata aaaatacatt gatcatttct gtcctaacaa
     5101 tgatcgtaca agtgactgta gtaactttgg cgggctatac ttatagtcgc tatcgtttta
     5161 tcggccgcaa atcaagtttg atgttctttt tagtgattca gatggtaccg actatggcag
     5221 cattgactgc cttttatgtc atggctttct tattagaagc gctcgataag tactggttct
     5281 tgacaatgat ctatataggc ggtggaattc caatgaatac ttggctgatg aaaggatact
     5341 ttgacactgt accaatagat ttagatgaat ctgctaaact tgatggggca gggcatttca
     5401 ggattttttt ccagattgta ttaccgcttg tcaaaccgat gctcgccgtt caagcattat
     5461 gggcttttat gggacctttt ggggatttca tgttagctaa gttcctgctt cgaacacctg
     5521 aaaatcagac tgttgctgtc ggcttacaaa catttatttc tgatgtgaca aaccaaagag
     5581 tctctctttt tgctgctggg gcaattttga ttgcactacc gatttgtcta ttattcttct
     5641 ttttacaaaa gaactttgtt accggcctga catctggcgg aacaaaagga taggagcaga
     5701 gtatcatgca aacatttccg ttaaactatt tttccgcatt acgtacgcca actcaagtgt
     5761 ttgcgggacg caaactatta tcatggccta aatttttctt gatatttgta tttttagttt
     5821 ccttgatggt catgcctgtg actctttttt atgccaatca gatccaagcg attccattag
     5881 aacaatttct atccgttcat tcgctgatcg atgaacaagg aacccaaaaa ttttctgaac
     5941 ttgaattatc tgaaactggg ttacaagcat cgcaacaaac gatagctgtg acccctgaaa
     6001 ttttggtagg tgtgtcttta tctgagaaac aacaatccga tcatggaact tttattgatt
     6061 ttgagaagga acaatgggtg atccaacaaa aggataaatc tggtatacgc cgctatacga
     6121 tgaattatag cccatctttc caaccagatt ctgtacgtac gcccgaagat ttccaacgat
     6181 ttttagaaag agaattttat gccagtaatc gtccaacaat cattttatct tatagtttaa
     6241 gtcttggtct tgtcctattt gtcatgacga gtctcatttt attcggggct tcctttttct
     6301 tgtggatgac aagaaaaagc caactttcat ccattcatac attcaaagag agtgcaaatt
     6361 tgatgctgaa tgttatggga ataggttcca tggcagcagc agttgtaggg tttatccact
     6421 ttgactttat cttgatgcta agtgtccaaa caacgataac tgtcttgctg cttttatggg
     6481 tttttgtcaa aacaagattc aaagataagc gtgtcgaatg acaagtttaa gcgatttatg
     6541 cgacaatgaa tgtgaaaaaa atgaaaaata aagtagtttt tgaaaaattt ctgaaaaaat
     6601 taaacttctt agttgattct tttctttttt agtattaacg taatagggta gtttttttaa
     6661 ggaggaaaat catgttagag aaaactttat acaatcaatt attttcacgt tctttctcgc
     6721 tccctgtcga agtaaattat tgggacggta caacgaaaca atacggagat acggacactc
     6781 ctccccaaat caaaatcaat atccatgaag aaatcccaat gaaagagatt acaagtaacg
     6841 cttctttggc actaggagaa gcctatatgg atcatcgaat cgagatcgag ggaagtatcc
     6901 aagcattgat caacgatgct tatcagcaag caaacagctt tttacgagga aaagattatc
     6961 taaaatggct gccaaagaaa gaaaaacata cgaaacaaaa aaacaaagaa gatatccatg
     7021 cgcattatga cttaggtaac gacttttata aactttggct agatccaact ttgacttatt
     7081 cttgtgctta tttcaataca cctgaagata cgttggaaca agcacaaatc aataaagtcc
     7141 atcatatctt agacaaactc tttatcaaag aaggcgaaac gttgcttgat atcggttgtg
     7201 gctggggaac attgatgttc actgctacca aagagtataa tgtcaaggca actgggatca
     7261 cactaagtga agaacaatac gatttgatta ccaaaagaat aaaagaagaa catttagaag
     7321 ataaatgccg tgtattatta atggattacc gcgaactaaa aggcgaaacg tttgatcata
     7381 tcacaagtgt aggtatgttc gaacatgtag gttctgaaaa tcttgaaggt tatttcaaag
     7441 tggtaaaaga tttattgaaa ccaaaaggaa cagctttgat ccatggaatc agccgacaac
     7501 aaggcggtgc gacaaatgct tggatcaata aatatatttt ccctggcggt tatgtccctg
     7561 gtgttactga attagttgat aatattacaa aaaacgattt gcaactgatt gatttggaaa
     7621 gcttacgtcg tgactaccaa ttgacattag aacattggac aaagaatttt catcaagtcc
     7681 aagatcaagt cacgaaagaa atgggtgatt ccttctatcg aatgtgggat ctatatttac
     7741 aagcttgtgc agcttcattt gaatcaagta atatcgatgt gattcaatat ttgcttgttc
     7801 aacctggaaa caatgacatc ccaatgcatc gtgcataatt gaggcaaaag tactgtttaa
     7861 agactattta aaaagaagct aagttttatg atgctccaga agtgaacttt tttgtctaac
     7921 ttctaggatt gtctctaaag cttagtttct ttttctttat attttcataa taaaagtttg
     7981 gttctttaat ttttaaacat gacagaaaat tttttcttcg ttatacttgt aatagaatac
     8041 tattttataa caaaggggat cgaatcaatg aaaaaaactg ttatttatac tagtttattt
     8101 gcagctttgt ttttagctgc ttgtcaatca aataccaatc aaggcacatc tgggaatacg
     8161 tcttccagta gcgaacagcg ctcttcacag gtatcggact cctcaacaaa agcaagcaca
     8221 tcttcgtcta gccagacaaa aagttctagt gaagcaagta ctcagtcaga tatcaaagtg
     8281 tctgcagctc gtgcgatcga attgttccaa gataaatacc cagatactgc aattactagt
     8341 ttggaattag atagtgattg gggatcttac ttctacaaga tagaaggtgt cgatgatcaa
     8401 aaagaatacg aagcaagaat tgatgctcaa aatgaaaaaa tggagacaga gaatccagaa
     8461 actctagatc gagatgaaca aaatggtcaa aagaaaaaag aagatggtct ggatgttgat
     8521 aaattgatca cgattgagga agcagcaaaa atcgccgtcg acaaagtagg aagcggtacg
     8581 gcaacagatt gggatttgga taaagattta ggtactactt attgggaagt caaagtaaaa
     8641 gacggcaatc aaagtacatc agtcaagatc aatagccaaa caggcgaagt actgtcaact
     8701 gaacaagatg attaagaatt tcttcggaaa acaagagttg atttttcttt tagtcatgat
     8761 acactagaca aaataacttg tttgaaggag cgatttactt gagtttagaa acgacctttg
     8821 ccaatcatat ctttgccaat cctttgatga atgcatctgg tgtccactgc atgactaccc
     8881 aggaattaga tgaactggct cattcagaag caggagcatt tatcactaag agctgtacga
     8941 tcaatgaacg aaaaggaaat cccgaaccac gctattttga tgttccttta gggagtatca
     9001 attctatggg acttccaaac ttaggttttt cttattatct agagtatgct cttgcgtatg
     9061 aaaaagtgca ggaaaaccaa aatcaaccgt tatttttttc cattgccggg atgagtgtcc
     9121 aggaaaactt ggaaatgctt gagaaaatag aaaaaagtgg gtttaatggg atcactgaat
     9181 taaatttatc ttgtcccaat gtgcctggaa aaccacaact agcttacgat tttgaggcca
     9241 cttatgaaac actgaaagaa gtattcagca tcttttctaa gccgctagga atcaaattgc
     9301 ctccttattt tgattttgct cattttgatc agatggctga tatcttgaat cagtttccat
     9361 tgacctatgt caatgctatc aacagtgtgg gcaatggttt gtatatcgat acagaacaag
     9421 aagcagtagt catcaaacca aaagaagggt tcggcggcat tggcggagag tatatcaagc
     9481 cgactgcttt ggcgaatgtt cgcgcttttt atacacgttt gaaaccagaa atccaaatca
     9541 ttggtacagg tgggattcgg acaggacaag atgcatttga acatctgcta tgtggcgcaa
     9601 gcatgttaca gattggtacc gagcttcata aagaagggcc ggaaattttt tctagaatca
     9661 taaaagaatt aacgcagatt atgtcagaaa aaggatatac gtcaattgac gaatttaaag
     9721 gcaaattacg caccatttca tagcaaagag acgaaggtca tgattgatct tcgtcttttt
     9781 ttataaaaaa cactcgtctg ttcattcctg tgcaagcgta ctcattgtat gttagacgaa
     9841 cttgtgggaa aataacagag gcaaaaagga ggataatatg aatgatgcaa cagaattagc
     9901 aaaagagctt aaagaaaaag aatttagttt tcaagaacta gttacggata tacagaaaaa
     9961 aataaagaag caaaatcatg aattgaatgc gtttgtcgcg cttgaaccat tcgagagtaa
    10021 ttcccacgag ctattgattc cccagtcaga aaaagggcca ttggctggta tcccatttcc
    10081 tttaaaaatg ctcggtcaag aaaaaaaggg atggctagct acttcaggat ctcgtttatt
    10141 tgaaacacac cgagcttctc atacatctaa ttatgtgcgg caagcagaag cgataggtct
    10201 agttcctttt ggacaaacca atgcgccaga gtttggtttc aaaaatatta cagacccagt
    10261 catctatggc ccagcaagaa acccttggaa ccttgaatgt acaccgggcg gatcaagtgg
    10321 aggagctgct gctgcagttt catcaggaat cgttccttta gctggtgcta gtgatggcgg
    10381 tggttctatt agaatacccg cttcgttttg cggattgatt gggctgaaac cttcgcgagg
    10441 gacgatgcca gttggtccat atgcttggag aggatggcaa ggagctgcta ttgatttcgg
    10501 cttgacagtt tctatgagag acacggaggc tttattttat gggatgagaa gtatccattc
    10561 cggtgcgcca tatcaagtat cgccagttga gtggcaaaca catccaagaa aaaaacggtt
    10621 gaagatagct ttttgtgttg attcaccgat ccactcacag attagtgaag aggcagtact
    10681 tgctgtaaaa aaagctgtag atttccttgg aaaagaaggg catgatttga cagagattcc
    10741 ttacccacta gatggtcgta aactgatcac gtcttattat cagatgaatg gcgcagaaac
    10801 agcagcaatg atccgctcga ttgaaacagg catccagcga gctgtcagaa aagaagaact
    10861 tgaacccatt tcttggactt tgttccaata tggtcagaag ctctctgccg ctacttacat
    10921 cgattctctg catgtatggg atgaagcagc aattgtcatg gaaaacctac atcaagaata
    10981 cgatctattc ctttcaccaa cgactgcatt taccgcccct aaaatcagcg aagatttgca
    11041 aagtgatgcg ataagagcac agatggaaca catcagtgat tgttctgaag tagaaggttt
    11101 agacgttgtt tcttccatgt ttgaggagag cttgaagatc acaccttata cacagttggc
    11161 taatctgact ggacaaccag caatcagctt gcctactcat ttgacaaaag aaaacatgcc
    11221 tctaggaatc caatttatgg ctgccagagg aagagaagat ttgctatttc aaatcggcta
    11281 tctatttgaa gcaaatcatc agttccattt accaaatcca tggaaatagc agaaacaagg
    11341 agaagaagaa tgaagaaaaa aagattgttt gctctaataa ctagtgcctt ttttatttta
    11401 agcgcttgcg gtacagctgc cgctaatcag gaagataaca gctggagtaa tgtagaaaaa
    11461 aagaaagaat tagtagtagc gacttctggc acactgtttc ccgcatctta ttataatgat
    11521 caaaatgaat tagttggtta tgatgtagat ttggcaaaag aggtggctaa acggttaaat
    11581 gtcaaaatct cctttaaaga atacaacgtg gatggacaaa tcacttctgt ttcaaaaaga
    11641 gaatcagatt ttgctgcgaa cgattttggt atcacaaaag aaagagaaaa aaaattctcc
    11701 ctttcagaga ctttcaaata ttcatttgac agtatgatcg tacgaaaaaa agatgattca
    11761 ggaatccact cacttgaaga tcttaaaggg aaaaaagcag ctggcgaacc caatactagc
    11821 tatatgcgtt tagcggaaaa acacggagct aaattagtca catatgacaa tgccacgaat
    11881 gatcagtatt taagcgatgt agcaaatggt cgaacagatg ttattctaaa tgattattat
    11941 ctgcagaaaa tggccgtatc tgctttacca gatatccctg taaaaatact ggagaacgtt
    12001 tatttcaatg aaaacgaaag tggtttttta ttgaaagaag gaaatgatgc tctgaaaaag
    12061 aaaatcgatg gcgtattaaa agagatgaaa gaagatggga cgacaaagaa aatatctgaa
    12121 aaatattttc aaactgatgt ttcagtcaaa ccgaaagaaa cgatggctga gtcggtgagt
    12181 gagtgatgta ctttcctcta attgattttt ccctcatgtg gacttcactt cctttcgtac
    12241 tgcaaggctt ggtatacaca ttagggattg gcttcgtgtc gtttctttta ggaaatctgg
    12301 tagggttgct tttgacagtt ttaggtcttt tggattggct gcctctgaat gtatttattc
    12361 gtttttattt atcgttcttt agaggcattc ccgcactagt cctgctgttt ctgttatatt
    12421 ttgggttacc ttatcaatta agtgccttaa cggcttcagt catctgcttt acgattacca
    12481 gcagtgcatt tattggtgaa atctatcgag gatcactagc aggcgtttct tcaggtcagt
    12541 gggacgctgg tttagcttct ggtttttctt tctttggggt attgaaatat atcgttttgc
    12601 cgcaagcttt tcgaatctca gtaccggcac tcagtaacgt agctatggat ctagtgaaag
    12661 gtacttcgtt agcagcaaca atcacagtgc cagaaatatt tcaaaaagca aagatcatag
    12721 gcggtcggac attcgattat ttttctctct atattttagt tgcactgatt tattgggtca
    12781 tttgtatcgg gatcgaaaaa gtgcaacagt atttagaaaa acgttctatg catcagtcaa
    12841 tctgaaaaat agaaatggtg ctagcgtggc tggcaccatt tctatttttt gtttcttgta
    12901 tattctgcct agtattttaa aaccatttca tgtaaaatgt aaatcgaaaa tatgttcgga
    12961 ggtagaaaac atgaagaccc cgctaaccat cgaaatagaa tcctcgttat accaatattg
    13021 catcgagcaa ggcggcttgg ttgtagaaga agtcacgatg cctgatgacc aaggaattgt
    13081 agatacgctt agctgcttga caaaatttga tggaagccga gaatggcgat gctatgagct
    13141 gaaagtgaca aaagcagatt ttcgctctac agccaaactg tcatttgttg gacattataa
    13201 ttattttgtt ttaccaagta aactatatga agaagtaaaa actgaaatcc ctacaggaat
    13261 aggtgtttta gtgtatcgtc cgtacacggt tgaagaggag atgcctgcct ctggtacatt
    13321 tctgattgcg aaaaaagcag caaagaaaga gcttgctgta ccggaagaag ctttgacaaa
    13381 ccgcttcatg gcttctttat tcagggaagt aagaaaagca aagcaaatgg agcgaggcgt
    13441 aaaatatttt ccaacagatc aattgtacaa agaattgaaa agacgttcag aaaatcaaga
    13501 tccattttct aacgaacgat attatgaacg gtttttagag gaaatagcta gtcaggcaat
    13561 caatgagttg caagaagaag ttatcgcgct tcagcatgat tatgaatatt tgaggcaaca
    13621 acagcagatc cgtcggttgc ctacagagcc attggagtga aaaatatgta ctatccttat
    13681 ttaagaggaa aacaatttga cctattagca ttaaaagaag cactgtcgcg aggattgctc
    13741 tccaataaaa tccaaccagt gatcgaacca gtacgtgatt ccgctacgct taaaaatgtg
    13801 atcgaattat ttcaaaaaaa gcaccatcca atcgctgtga tccaaaatcc ccaagtagga
    13861 caattcaaat tattcgatca gcatgtgcac agttggacag tcaaagaaaa tagctctgtc
    13921 gtccctgcac agatcttaac gccagaaaat ctcagtgaag tactggattc acctccagcg
    13981 tttcttgttt tcgatggaca gcactaccca aaagatacag aggtgtggaa acagttggca
    14041 ggagttgatt cgaaattttt gataccagat actagtcgct ttcgcatctg gctgcccgaa
    14101 aacaaaattg tgatccgtga ctcttttcaa acgagaaagc atgttgaaag ttatgcagat
    14161 aaaacagatg acttttttag tgatgactat ctttttttcc acgcagacgg atatattgga
    14221 ttttctgatt tcacgatcga aggtagccgt tatttcgata aagggttccc tagccgagcg
    14281 cttgctatcc atctaacgta tattgatgct tacggcaata tacgcgtgaa acattttgtc
    14341 tctgacagta acgactctgc taaagaccaa gcattgaagt ttcttgaagc aggagataaa
    14401 atgaaaaaat ggatcatgcg tcaccatcat caactcttga ttacttcagg aatgattgaa
    14461 ttattggctc tttaccagca acaaaaattc cctggactgg gagtgttgaa aaagtggtca
    14521 cttatgcatc atttggaact tattagtcac ctgctagatc atccaaaaga ttggcttaaa
    14581 tattattcaa aagttcagga actatatgaa gtgatccaat gattagataa tgtaaatgaa
    14641 aaaggaatag aaaagaggaa actggatgta tattcggcaa acaaggaaag aagaagtaga
    14701 agaagtggta gaaatcatta atgccgcagc ggatttcatt gcttcgcaag gctctcctca
    14761 atggcaaaac gggcagaaac caacaaaaga aacgattttg aacgatattg aaagaaaaga
    14821 aagctatgta ttaatagtgg aagatcaaat tgctggaaca gctgcattag tctcaagcgt
    14881 ggatcccgtt tacacaaaaa tagatggtag ttggaaagaa tcaactggtc cttatttatc
    14941 gattcatcgt gtcgctttag cagaaagtac tcgaggaaaa aaactagcaa aaccgttctt
    15001 agaacatttg ttgacgattg cagccagcaa aaatatccaa gatatccgta tcgatactta
    15061 tccagctaat gaaccgatgg aaaagaccat ctattctgct ggattcactt atcaagggat
    15121 gattgaattt ccttttgaac acggcgaaag aaaagcatat caaataatca tcaattgact
    15181 ataaaatcat ctgagcaaag ataaagacat taacactact gaaaaaaaca atcatatgat
    15241 agccaagagg ttgtaacaga agtggccagc ttcaaaaaac aagaaagaat ttccgaaaat
    15301 gcttctcaaa tttttgtgga attttagcgt attttacgaa atagctgctt tctgaaactc
    15361 ccttcatcga gaggtcgtga catagctttt gtcacgacct ctcggcttat cctacgagta
    15421 ttattcgata catttatatc aataatcctt tttttatttt atctgcagct tcttggaacg
    15481 ctccatcgtt tgacacgact aaaagtgagg ggatagcttt caaacgtttc aactgtgcta
    15541 aatcttctac ataaaccgcc agtctggctg aaatctgttc agatgaatct ccacgttttt
    15601 tcaggcgcga ttgcaatgtt tctttagaga tcgtaataaa gataggacgg actaccgtcc
    15661 cgaaatactc atataaatgc caaaaaccat ttgctgtcaa aacggcgtaa caatttccta
    15721 ctgtcagttt ctttttaatt tctgctttac tggaaccata tttatgttga gcatattggt
    15781 cccactctgc aaactcattt cccttgatca tctgattgaa tgtattgtca gaaacaaaat
    15841 aataatcctc ttgatctttt tccccaggtc ttggtgatcg attcgtgtaa gtaatgatct
    15901 tttgttccgg ctgaaaaata gcttctgcga gtgaagtttt tccagaacca gaaggaccga
    15961 taaatacata gcaataattc attgtcattt ctcctttgat gattgaacaa gtgaaaaagc
    16021 tttggaaaat atttccaaag cttttaaaag taactttgct tttttgtata ttataccagc
    16081 aagccagtca caatagcact taaaaagctg actaaagtcg ctgttagcaa aattttcaat
    16141 ccgtgttttg cgacaagatt tcctttttct tcatttaatc ccttcatcgc accagaaatg
    16201 atcccgatag aagagaagtt ggcaaatgaa acgaggaaga cagatataat tgctgttgta
    16261 cgctcagtaa aatggaaatt accatttgct aattctgtca tagcaacaaa ttcatttgta
    16321 accagtttag tcgccatgat actaccggca tccactgctt ctttccatgg gattccgctg
    16381 atgaaggcaa gaggggcaaa gatgtaacct aggatctctt ggaaagtgat gccgaatatc
    16441 ccgtggaaga tagcattgat catggcaatc aaagcaacaa aaccgataag catagcagca
    16501 accgtgatcg ccacatggaa accatcaaga atgtattctc ccaacatttg gaaaaatgtc
    16561 tgttctttgt tttcttcaat gatcagctca tcttcttttt cttctaatac atagggattt
    16621 acaattgaag ccagaatata accgccaaat aagttcaaaa caagggcagt gatcacatat
    16681 ttagaatcga tcagtgccat ataagatccg acgatcgaca tagaaactgt agacatagcc
    16741 gatacactca tggtgaacaa acgtttctca gataggaagg gtaattcctt ttttatggaa
    16801 ataaaaactt ccgattgtcc taaaatagca gaagctactc cattataaga ttccaatttg
    16861 cccaacccgt tgattttact taatcctaga ccaacagctt tcataaataa tggaagaatc
    16921 ttgatatagc gtagaatacc gataatagca gaaataaata caattggcat caatacggaa
    16981 agaaagaaag gtgcacttcc tttatttgct acaccggcaa aaacaaagtc aacaccttgc
    17041 ccagcaaaag caagaagatg atcaaacatt tttgccaata cagaaacaac cgctgtacca
    17101 aaagttgtac gtaataatac gaaaccaaga acaagctgta aaacgaacat cacaattagt
    17161 ggtttcaatt tgattgcttt tttgttactg ctgatcaaaa aagcaagccc taatgtgaca
    17221 aataaaccaa taatacctaa taaataactc aaataatcac tcctattcca aattccgttt
    17281 tattttacct gttttgcaaa taaaatacta atctttagtg aaattttcat tactttttta
    17341 gagtgaaacg cattcatttt ccattagaaa ataaagcgaa caaaataaaa ggtttaaaaa
    17401 gtgtaacgtc ttagtaaagt ctataaaaaa acgaaactat cgattaagct aatagtttcg
    17461 ttttcaacat ttataagtaa actttttcta ttggtaatat agattttcgg aagggtagac
    17521 aggtgcgcag gtaatatcga ttcctagttt tttcaatgtt tgttcatcat ttttattgac
    17581 catcacagta gagtgggctt gtgctccctc aagttcagct agtttatcat aagcaacttg
    17641 agcgattgga ttcgttactg cactgattga taaagcgatc aagacttcat tgatgcttag
    17701 tgcatccaaa gaggaatgaa gtgtctttga tttcaatcct tgtattgttt caagaactaa
    17761 tggtgacaat aaaggaatgt catctgcgat atgggctaaa tgttttactg catttaagat
    17821 aactgccgct gacgcatcca tcaatgaact tgtacgcccg gtaatgatga cttcatctgg
    17881 taattcgatt gcaatcacag cagaaggatc ttttgtagga ctttgcgctt gaatcttatc
    17941 tgcatagtca tgggcaggga ctaccacgtt tcggtcttca ggacgtaaac cgacttcttc
    18001 cataatcagt ttgattcgat taactgcttc ttcgtcgatc aatccttttt tgaagtcgca
    18061 ttctgtttgg aaataacgac ggatgatttc ttgtttagag gcttcttgga caacggtatc
    18121 atccacgatc ccaaatccta cacgattcac acccatgtca gtaggagatt gataaacaga
    18181 ttctttacca gtaatttttt caataatacg tttgattaca ggaaatgtct caacatcccg
    18241 attatagttc acagctactt tattgtaggc atcaaagtgg aaggaatcga tcatattgac
    18301 atcttttaga tcgactgtgg ctgcttcata agcaatgttc agcggatgtt tcaacggaac
    18361 attccatact gggaaagttt cgaacttgga gtaaccagct gcatgtcctt tttggctttc
    18421 atgatacagt tggttcaagg aagtagcgag ctttccactt cctgggccag gagctgtcac
    18481 tacaacgatt ggtttagttg tttcaatata ctcatttttt ccaaaaccat cttctgagac
    18541 gattttttct acgttgactg gatagtcttc gatttctgag tgtttataga ccttgatgtt
    18601 tcgtctttct agtttattga taaagacttt cgtggcaggc tgtccactat agcgagtgat
    18661 aacgacactg ttgattaata agccataacc acgtaattca tcgattaacc gcaagatatc
    18721 catatcataa gtaatcccat aatcaccacg gatcttatta cgttcaatat ctccagcata
    18781 gacgcaaatc agtatttcag cttgatcttt caatttttgc agcaatttga ttttcgcatc
    18841 ttcttcaaat cctggaagta cacgtttcgc atgcttgtcg tcaactagct tcccaccaaa
    18901 ttctaaatat aatttgtcat agtggttcac tcgttccaaa atatacgctg actgctcttc
    18961 aatatatttt tctgaatcaa aaccaatttt tttcactagg ttccctcctt aagtactaaa
    19021 gcattttttc atttttttga ggattcgtca actggaagag cgaaattcct caaaaaaaca
    19081 aagaaaatgg tttaaaccaa cttgtctaga tagttattca ttggtatgat agtttttgat
    19141 aaggaaggtg ctacatgaaa aaaagacaga aaaagaaaaa cgcatataaa cactatatcc
    19201 gttcgatttt taccggttat gaaagaatgc ttgaagatcc cgaattggaa caattgacgt
    19261 ttacttatct aaacgaagaa acgcagctaa ctcgtgatga gcatcagcgc attcatttca
    19321 caacaagaga tctgcctagt aaataaaaaa taagataaag gtcatcggat tcgattctgt
    19381 cctatcagat ggaaagacta gttgatcgaa aaaaaggctg taccaaattt tggtgcagcc
    19441 tgttttatgg agcattgccc gctctttttt ataacaattt tttacagaaa atggtactat
    19501 tgaagtatta ataagtgaaa aacagatgag taagaggagg caagtagtat gaaagagcca
    19561 gatacaaatg caaattatga gccagacgaa ctagaggaga cgaatgcgga tcaggataca
    19621 ggacaagaat cgtcttcttt ttacgatatc aaagaaaaag caagcatgaa tctttcccaa
    19681 ttattagaag acttacaggc ttttgaaaaa gctgtccgcg aagaagatat ccctgagatc
    19741 taccgtttat acaatggccg attgaatgaa gaattaaaaa gaagctcaaa tgaaaatcac
    19801 gagatcgatc aattgctgat gcgtaagatc catgatcgtt ttttacagga atttcctttt
    19861 atggaacaag tagaaaacat ctcgccaacg atgagctatt acaaaatcgg cacttattac
    19921 catgatcggc ccacggtcgg tattgatgca agtctgccag aaatttttgt tttgcctaag
    19981 atcgatgaag agtgggagaa atattcccaa ccggagtcaa cggactatga caaaaaaatc
    20041 aatgagctag acgcaaaggt gatcactgct cagacagaaa ttgaacgttt agatgaacaa
    20101 ataaaagaaa tcaatcgaca aatgactgat ttgaacgaca ataaaggatt cttgaatcgt
    20161 aaaaaaatcg aggaagaaat tcaagaatta gagaagaaga agcaagtact atctaatgaa
    20221 aaattaggct ggcttcctta tattgagacg cctgaaacaa tccagcagca aaaagaagca
    20281 ttgaagcaag aagcaagagc agaccagctt cgtgcagcca tcgtagaaaa agaacaaagg
    20341 caaatcagac gctatttcgg tagcaaagag ggattcggtc aagcaatcca tgaattcttg
    20401 atgaattatc ttggcaatga gaatcctaat aatcaatcta atgaaggagg ttccgagtat
    20461 gaatgaacac gatgaagcag aatggacaaa aaatgaagaa caaacagaag atcatgaaga
    20521 aacgaacact tcattggaag aacaaacaag taatcctctg gatgacacag aagaaatccc
    20581 tatgctagag gaaacaaaaa tacacgaaaa agaaaaagta actccgacga atacgaatac
    20641 tgcagaaaac acccatgctt acgaaataac tgaaaatccc gggtatttag aaggaaaaga
    20701 gcaagatcat caagatatcg tcagtttgat tgaaacagcg gaagcagaat tagcggattt
    20761 acgtatgaga aaagatttga tcgaatcaga ttttccacaa ttattagaat catatgatcg
    20821 ttttatattg aatgaagaac caatctccac gatcggaaag acaagcctga tcgaatactt
    20881 cacgtatgaa ttactgaaac cgttgaatga tattggtctg gaacggtcaa attcagctta
    20941 tgacacatgg aaaacaagac atttttcgat caattatacg ttgaatggac aaaacgaatt
    21001 agtcttttcc tttgtattac cgaccatcga tcaacgatac caagtggaat cgatgagtct
    21061 cctaaacgta tcaccagaga caatggaaat caatgtccaa gatgaccggg tgctttcttt
    21121 gatccgacac tggtcagtgg atcgagtatt ctctgcaggg cagatcacga tattcaatca
    21181 caaattgaat caattattag cccatgcaag aacacttggt tttaccgtta atcagacatt
    21241 actagacaat acgaagccac ttcgtttgaa tcttcagagc gaatttgaat tgacgaatga
    21301 agtattggat gatattttta tcgtggcaat gaaaaatcca tcctacgata tggaaaagat
    21361 caacgaaact acttatcaag tacgattaga taaaggacag tctttaacga taacaacaaa
    21421 cgaaaaagat caaacagtct tgtcgatctc atctggagac tacccacgat ctgtcattga
    21481 tttctttatc aattacgaat tcttggtccc attaatggtg agaaagtcct gattggataa
    21541 gttggattac cagatgtcat tcagataatt ctgttccaaa ggacttgcgg cagttatcca
    21601 gaataattgt cacaagtctt ttctgtatgt actaaatgga cgtgaataac ttgctgatga
    21661 aggagggatt tcattaggaa tgtccacaaa taaaatagca cagcttagct tattgtctgc
    21721 tgcttgtgta gcaggtagga tcgcttttca atttattccg aatttccagc caatgactgc
    21781 tattttttta tttatagtac tttacttaag cttaaaagat gcgttaattg tcatgagtct
    21841 ttctattgcg atcagcagtt tttattttgg tgttggacca tgggtcatcg gccaatggat
    21901 cagctatact gttgttctga gtttgttttc aatcgtctgc ttgagaaaaa cagtgcaaag
    21961 gaatctatgg ttaaagggag tctgtttttt tctggcgggc atggtttacg gaataggtat
    22021 gaccgttttt gatacgctgt tatatcgttt gcctcaacct tggatctatt accttcaagg
    22081 ggtgtcgttt gatttgatgc acagtatagg caatgttgtg ttctttcttg tttttctgcc
    22141 agtcgtcaaa cgtttttaca atcattcagg aggaaaaaat gaaaaaaata atttatagtt
    22201 tgcttgcaat gagtgtcagt atactcattt tgacaagttg cagtacaacc tatacacaat
    22261 cggcttccaa ccaaaaggag gaagtaaaaa catcgcaaaa agcaacaatc acgttacaag
    22321 aaaatggtca aaacttttcg gaaaaagaag tgttcttcaa aaaaggcgat gatttactga
    22381 gtattttgaa acgaaatttt gaagtcaaag aagataacgg cttcatcact tcgcttgaag
    22441 ggcactcaca ggatgagaaa aatcaactct attggatgtt tactgtggac gggaaaagtg
    22501 ctactactgg tgccaaagag atcaaattga atgatggaca atcggtcatt ttcaatttgt
    22561 ctaaactata agtttcataa aaaactgtta aagggcgaat attgccataa ttcagtgagt
    22621 tcagttgatg aaagaaggaa aataacatgg aaaattttaa aaaaagcgga atgttttccg
    22681 taattgctgc tctaggggct aatattctag ttgctatcag caaatttgtc ggctatgcaa
    22741 tcagtggcag tgccgccatg ctaaacgaaa gcatccacag tatcgttgat tgcagcaatc
    22801 agattttgct gttattcggt gataaacaag ccaataaagg acagagcgag ttgcatcaat
    22861 ttggtgaagg acgagcaaaa tactttttca gtacaatcgt cgcaatgatg cttttcttcg
    22921 gcggtggggc acttggggtg atggaagctt ttgaaaagtt gacgcatcca tcccatgaag
    22981 tgggtaatcc gatcattgtt atagcaatac tgttatttgg tttggtaatc gaaggaagtt
    23041 cgttacgcgt ggcaatgaaa gaaatcaaag aattgaatac tgaaaaactt tccacgatgc
    23101 gctttttgcg cgaaagtcga cacagtgaaa tactgattat ttttacggaa gacttgtgtg
    23161 ctgtaatcgg tttggtcttg gctttagcag gaacaatatt gacttatgca accgggattg
    23221 ctgcatttga tgcgatcagc ggattgctga tcggtttctt gctgatggga gccgccattt
    23281 tcctagcaaa agaattttat agtctgatca tcggtgaaag cgtgacagca agcgatttat
    23341 caaaaatcaa gttagctttt gaacgtcctg agatcagtcg gctgatcgat ataaaaacag
    23401 ttcatttaag tccaacggag attttagttg cagcaaaagc agatatcgtt ggtaacaaag
    23461 agaatgaagc ttactcggtc atcaatgata ttgaaaaaaa tatgcgtcag tctcttcctg
    23521 acaagaagat gtatatatac attgaaacag ataaatatga cccaaattat tctcgtaaat
    23581 aaaacgttta ttcatgaatt gaagcaaaaa tgagaaaaat ataatcttgt ttttaaataa
    23641 aacggctaca atttagcgaa tttatgttac aatgagtccg ctgtttattt gttgtaatta
    23701 aaggagatat tttaatgaac aaaaagaaaa gtgcgccatg gcttcaagct ttgcatgcag
    23761 tgatgccact ttgtataagc tatataccag taggattagc ttgcggagta ttgttgcaaa
    23821 aagttggatt tgatcctctc ttatcaggac tgttatcgat tctggtcttt tccggtggcg
    23881 cgcagttttt agttgcatct atgcttacga cgcaagcatc atttgcaacg acattattga
    23941 tggttttctt tttagaatta cgctatacat tattaggagc aagtcttgcc ggttttatga
    24001 aaaaagaaaa gcggacgttt ttggctgtct tttctcaatc actgaatgat gaaaactatg
    24061 cggtgaacta cttaaagttt tcaactgacc cttcctggaa taaaagaaag gctttatatg
    24121 tcaattggtt ctcaatgact gcatgggccg gtagcaatat gatcggtaat tttttcggct
    24181 cggtgattcg agtagatgct gaccttgtcc attttgcttt gacggccatg ttcattttca
    24241 tgtttatcat gcagatgaaa agcgcactac tgatctttac aggtttattc tcaggcgtat
    24301 taggggtcgt attcatggta ttattccaaa atactttcgg attgattgca gcaacagtca
    24361 ttgcttcatt tgccggattt atgctggaga aggcattcaa aaaagaaaaa gctaagaaac
    24421 taagagaccg cggtaaagat gtcaatgagc cgctcttcaa cttcccggaa caggaggcca
    24481 atcaccatga ctaaactata tttgctactt ttagttgctt gtctgttcat cgtggcatat
    24541 atcccgcgct tgttcccgat gctttatttt actcatcgaa aagtgccatc ctggttcagt
    24601 gactggatga aatatgtacc ggtggcatta tttgcggcgt tagcttttaa agatgttttc
    24661 attactcatg aacatctgga tattgcttgg aatatcaaaa tagctgcaat gattctagta
    24721 gcaggcgtgg cttacaaaac acgttcgatg gcgttgtcag ttttgacagg acttgcgtct
    24781 gtctttttgc tgtctatgct ttaaactgtt ttattttatt aaaaacagag attgcgacat
    24841 aagttgttca aactgatcta tccgaactgc atttgcaaca gtcaattctg gatgcaagtt
    24901 tattcgtagg gataaaaaag attagtgttg gcttatgatg caagctcttt tttattttag
    24961 gaattagaga gggatttgtc cttctatgat tttttgctat aatagggaag atgtttattt
    25021 tatgaaagaa tagttgaggg aatttaaatg aataaaaaat tgatgcaagg aacattttgg
    25081 ctaacctttg ctaatcttct atgtaaagtt ttaggggtag tctatttgat tccctggcta
    25141 agtatgatgg ggaacaatca agatggtatg cttgctacga cattgtataa tgttggctat
    25201 cttccttatg gattgttcct gatgcttggt acagtcggat ttccaaatgc gattgcaaaa
    25261 aaagtagctg tagccacaaa agaaggagat aacgctgctt gtcggctgat ttttagaagt
    25321 acgatcaata tcatgtttgt catcggtatc gcctctgctg cactgatgta tttatttgct
    25381 cctttactag ccggaatcag tccgattgct aacgttaata atgggatact agctatcaga
    25441 agtttatgcc catctttgat tgctatccca attcttagtg caatgagagg atatttccaa
    25501 ggaaaaaatg atctgcggcc ttatggaact tctttgatta tcgaacaggc tgtgcgagtg
    25561 atcgttatct tggctggtac ttattacttg cgcgtattga ctgacggaac catattagaa
    25621 gctgtattga tcagtacagt cgcttctttc ttcggtgggt tagctgcgat tatccatatg
    25681 tatatcgtcg gacgcagaaa agacttcttc gaacttaaag attttttgat ttccagccgt
    25741 tatttcgaaa gagaaaaccg atctgcctct gtctctatca ttcgggaaac actaccgttt
    25801 atttttgtcg gctcagtcat tactatcgtt caaatgatcg accaagtaac gatgaaacca
    25861 ctgcttcact ttttccgtcc agagatcgca gatcagcaac tggaattctt attcagccgt
    25921 gcttctgtca atccaaataa attgacattg atcctgatct ccatggtagg aacagtagct
    25981 ataagcagct tgccaatatt aagcacgatg aagaaaaaag atcggataca aatcgaaaaa
    26041 acagtaggag atagtttttc gattgcgtta ttgattcttc ttccttcatt gactggaatg
    26101 tctttattag ctggaccact atacacattg ttttttggct atgatcctga aagtgtcgga
    26161 tatttccaaa tggctttgct agcttctttg tttttctctt tattcacgat cctatctacg
    26221 atgcttcaat ctttgaatca ccatcgtttt gcgatccgat taactgtaga agcaattatc
    26281 ctgaaagtca tcttccaagt aatcggatta ggattagttg gcggatacgg gatgagctta
    26341 tcaaatggct tagcattcgg tattgtcttt gttcgcggct atcactttat gtgcaaagaa
    26401 taccgaatag cacctttagc aaagatcagc aattttttct taaaaacttt ccgctccacc
    26461 ctgattatgc ttgcagtatg ttttgccgtt ttctttttgc tgaatcagcg attgtcaatg
    26521 gaatcaaaaa gctatgcaat gatttattgt gtggctgtag gaagtattgg cggcttagta
    26581 tttttatttt ctcaattcgg aaaaaatggg tttcgaatgt tgaaaaatgt taaacatcat
    26641 agcagaagca aagaataggt taactaatcg ttttttgctg tcacttgaaa aacggcaact
    26701 cgtatgatta cgcttgtcgt ttttctattt tccaatttta gtttcatatc taatcttact
    26761 tatgatatag ttataaggtc tggaaaaatt ctttgaaacg aggaaattaa acacatgaaa
    26821 cttgcagtag ttacagatag ttcggcatat ctgccggaac gtatcaagaa ccatcccgac
    26881 ttattcgtta ttccaatccc tttgattatg gatgggaaaa tttataacga aggaatcgat
    26941 atcgaagcgg atgaatatta tggactttta aaaaacagta aagattttcc tactacttca
    27001 caacctgtcg taggggaagt tttgggttta tatgaggaat tgaaagagaa agggtatgac
    27061 acgattttaa gtatccatct ttcttcaggg atttctggct ttgtcaacac attatttgcg
    27121 atgaaagatg acatcagcgg agtaacggtc attccttatg actctaagat cactagtatg
    27181 ccgatgggac acatggtaga agctgcctta gacttaaatg atgaaggtca gtcccttgat
    27241 gaaatacttg cacatatcga tcgaatccga gataatacct acgcttatct gattgtagat
    27301 gatttgaaca atttagtacg aggaggacgt ttgacaaatg gtgcagcgct gattggcagc
    27361 ttattaaaaa tcaaaccgat cctgacattt tctgaaggga aaattgtttt attcgaaaaa
    27421 atccgttcaa gtaaaaaagc atttgctcga gctgaagaaa ttattggtag aagaaataac
    27481 gaaatacaac cgccagtcaa actttacgtg atacatgcaa ataatttaga agtagcgatt
    27541 gcggagaaag agaaattgca gaaaaaatat ccaaatgcag aaatcgaaat tggtcatttc
    27601 ggaccagtca ttggtacaca tttaggtgaa aaagccatcg gcattgccat ttcagcgcaa
    27661 taattccttt tattcaaaaa acataccaaa aatattccaa catcatcttg gactttctac
    27721 atgttactaa tgagcgtgta aaagaacgag atggtgtttt tatttctatc aatgaagctc
    27781 tttttctttg caatcctcac ttcaatcctc tataatgtcg catatagttt agtaaatgag
    27841 taaactacta atttacttat ttactaaaaa taaaaggatg aggagttaaa ataatggaaa
    27901 taccaacaaa tttaaaacat aaaccagtaa tcgttgtaga agactatgat aaagttgatg
    27961 gaagaaatgc attaaatacg gatgcaaaag ggctttcact gggccttgct cagtggaatg
    28021 atcgtggaaa agtcgatatc tcaggcaagg tatggcgaca tactggagaa aaatggtcac
    28081 gacagtcgga agaattacca atcacacgta ttttagattt agcgatctta gctgcacaag
    28141 gaagtttata cttccaagat gcttatcgtt atgaaaaatt ttatgatcca gaaaatccag
    28201 tcgtagacat cattggatta caagggaacc gaatgacggt agaagtagat accaaaaatc
    28261 caaagatcga tgaagacatc attttgtatt acgatacact acaaaaagac ggagaactga
    28321 ttggggaacg attccgtatt ttaaaacgtc ttttagatga gctaggatat taatagacag
    28381 agatgatcaa aaggggaaga gaagcatgga gaatttctca ctaacagaaa accgaacctt
    28441 ttcacggcta gaaaagcaaa tgatctggaa gaaaccgaaa agccatcgga caagtgcaga
    28501 agaatggcgg atcgcctccg aaatcaaagg gaattggcaa gatcctgaaa tgaaaatcag
    28561 taacgtactt ttggaaggag atgccgggtc ggggaagaca caattagcca aagcactatc
    28621 tgctgatctg caacttccct atacaaaagt gacctgtttt gcagatatgg acaaatcaga
    28681 tgttttcgga gccttattac ctgtagtaga ttctaataag gaacaagacc aagaattact
    28741 agaagctatt taccaaacag atacacttga agctgtactg acactgatcc agaaccatta
    28801 cggcattgat cctttttctg ctaaagaaaa attagcagag ctagttcaaa ggatcgaagc
    28861 tgattatcca gatgcaatcc attacagcta ttacccttct gaaatcgttc gagcgattga
    28921 aaaaggatat ttattagaga ttcaagagcc gactgtcatc cgagatgcct ctgttcttgt
    28981 ggcacttaac tcagcgctcg aaccaaatgg gatgttgaat ttaccgacag gaatcattcg
    29041 tcgccatcca gattgcgtca ttgtgatcac tacaaatcga aattaccaag gaaatcgccc
    29101 attaaatgaa tctttaagag atcggatgca acatgcggaa aaaatggatc ttcctgcctt
    29161 agaagtcatg gcagaacgtg cgatggcgaa aactatgatc caaaaacaag aacttctctt
    29221 aaagatggca gagattatcc gtttgttgga tgaaactgca aaagctaacg ccatcaaagg
    29281 tgtagcagga atgcgttctt atttttattg ggcaaatacc tataagcaag gacaagattt
    29341 actccagtcg atttatccaa aagttttgta taaattatcc actgattcag atgaattggc
    29401 aattcttgaa caagctttgt ctgacagtgg attgctactg gaacttcagg acatattgag
    29461 aaaagaaaaa tggggtcatc ctcaatctga gcatacaaaa ggaagaacca ttagcgccga
    29521 agaagcaaat gaacgaaaca tcactgaaaa tgctgaagaa attctgcgct tagatgaaaa
    29581 gaaatcgttg tctgctgaag aaccaaatga gggagaagag aaacaagaag aaacaataga
    29641 aaaaaacgag attcctcctc aaatgcaaaa agaacgcagt agcgatatcg aaggaaaaaa
    29701 tcaaaaagaa caacaaacag gagatgattc tcaagaaatg tctgcttctg acatggaagc
    29761 acatgataag gaaatcaaga agcaactgaa taaagaagcg cgacaaatca tgaaggaaac
    29821 gattcatgaa aaagaaggtc tcatcgttca ccgaccagct ttccaaggaa aaacgccgga
    29881 agcacgtgag ctacacatgc aagtaatgcc aatagtagaa tcacttagcc ggcaaatttt
    29941 agagttactg gataatgaac aaacagagac gtaccaaaaa ggaaaatacg aaggtcagcg
    30001 gttcaatgca agcaaagtag catatggtga tttgagaaat tttgacaaaa agaacccgcc
    30061 acatgagcaa ccttctttag cggtagctgt acggatcgat gaatctgggt caatgatacg
    30121 ggatgatcgg atcgaagcgg caaaaaaagc agcgattgcc attgccgaat tttctaagaa
    30181 agtcgatatc ccattgttga tttacggaga tacagcagac cgttcgtcaa gagaaaagac
    30241 ttctttgttt tcttataaag aatttgaaga cggatttcaa tggttaaatg aaaaattagt
    30301 cactatgaaa ccaaggcaaa acaatcgtga tggtgctgca cttcgtttag cagcagaaaa
    30361 attaaacaga caatccgcaa caaccaaact gttgcttaat attagtgacg gtcagccaaa
    30421 agcgttgccg gattatacag gcaaaaaagc aaaagaagat atccagtctg ttttgaaaga
    30481 atatgaacgt caaggaatcc tatttgtctc cgcagctatt ggccaagaca aagaagaaat
    30541 caagtcgatc tacggtgaaa gtcgttttgt agatattact gacttgaatg aatttcccaa
    30601 gcaaatgatt caactgattg cccgctatct atgataaaat aaagctcaac gaacgcactt
    30661 agcagattcg ttgagctttt cgtttctagt taccggtaac aaggatactt ctaaaataga
    30721 atcggttata atcaagaggg accgttaaag gagtgaaacg atatgataca agaacaaaaa
    30781 tggtggaaag aagcagtagg ctatcaaatc tatcctgcta gtttcaaaga tagcaatggc
    30841 gatggtcaag gagacatcaa tgggatccgt gaaaaactta attatttgaa agatcttggt
    30901 atcggcttta tctggatcaa tccgatctat caatctccat ttgtcgataa tggttatgac
    30961 gtaagtgact atcaagatat agaagagaga ttcggaacga tggaagattt tgatttattg
    31021 ctaaaagaag cacatgaatt agggatcaaa gtgattatgg atcttgtcat caaccatagt
    31081 tcggatcagc atcaatggtt tgaagaatcg aaaaaatcta aagaaagccc gtatcgcgat
    31141 tattacattt gggtcgacgg ggtagatggc aaagaaccga ataattggac atcgattttc
    31201 ggcggttctg cttgggagta ttctcatgaa actggacagt attatctgca tgtttttgca
    31261 aaagaacaac cagacttaaa ttgggaaagc gagaaattga aagaagaact attcaatatg
    31321 atccgatggt ggttggataa agggatagat ggatttcgtt tagatgcgat ttctcatgta
    31381 aaaaaagatg aatattcagt aaaagccaca gagaaccctt tctcaccctt tcaaaatgtc
    31441 tcaggaatcg aagaacattt gaccgatttg aaacatgttt ttgaagagta cgatatcatg
    31501 actgtcggag aagcaagtgg tgtcactgca gaagaaggac cacaatgggt agggaaagac
    31561 ggttatttcg atatgatatt cgaatttgat catatccata tctggcagca agaaaaagaa
    31621 gggcaattag atgtccttaa attgaaactt gcattaagcg cttggcaaac ttcattggat
    31681 ggtatcggat ggaacgcttt atatatggaa aaccatgatg ttcctcgtgc cgtttcagtg
    31741 tttggagata ctcgtcccga tttttgggct atgtctgcaa aagcaatcgc tatgatgtat
    31801 ttctttttgc aaggtacgcc atttatttat caaggacagg aaatcggaat gacgaatatg
    31861 ccctttgaat cgatcgatca agttgatgca gtagattcga aaagacttta taaaaggctg
    31921 cttgcggaag gtaagacaag agaagaagca ttggatatca ttcgtgaaac aacgagagac
    31981 aacagcagga cacccatgca atggacttcg gaacaatatg ctggtttttc tacacatgaa
    32041 ccttggctga tcactaatcc aaataccaaa acgatcaatg tagaacaaca agaatatgaa
    32101 ccagagtcgg tcctgcaatt ctataagaac atgatccgca ttagacaaac caataaaggt
    32161 ctgatatacg gttcttataa agaatatctt catgaacatc cacaacttta tgtctatgaa
    32221 agatatttag aagatgaaca atatcttatt atggtaaact taacagaatc acttgctgac
    32281 tatgaacttc caaaagaagc agatcagtcc tggacattac tcttgtcaaa ttcctctagc
    32341 ggagaatttg aagctaaagg catactagca ccttatgaag ctcggttata taaaacaaac
    32401 aagtaaaact tcctcgttaa agttcattag ttggactttg cgaggaagtt tttcagattg
    32461 ctctgtcaat tactcatttc aataattcgc tctaggacca ccgatatatt taggacgaga
    32521 gcgaagggct gtcacgtgag cttgtccaag ggttttcaaa cgaggtctga caggtacttg
    32581 gacatgtttg atatgcatac cgattgatgt gtcaccgata tcgataccgg cttgtccaac
    32641 tacgtgttcc acttcgacag gatcattgaa ctggtcaaaa gcagcaacag aacaagcacc
    32701 tccagcatgt agtgcaggaa caacagagac aatttcgaaa ttttttgctt ctgccactgt
    32761 tcgttctact actaatgctc ggttcaaatg ttcacaacct tggacagcta gcgcaatttc
    32821 tttaggatcc aacaactctt ttaacgtacg gatgatccat tgaccaactt ccgcactgga
    32881 attttttcca atatgtcctc caacgacttc gcttgtgctg catcctaaaa caaaaagatc
    32941 accttttttc aagtgtgcct cagccaatat gtcattaact atttcagtca attggtcttt
    33001 taattctttt tctgacaact tcattttcta aaaccttctt tttcaattaa tattgcttcc
    33061 gtgcttgaat acgttgaaga cttgcagtta taggaaaggc aagtcccatt ccaatcagat
    33121 tttggaagat gtttcctggt atagaagcca ctccagccgg ccaatcatat aaaatccaac
    33181 cggcgatacc atagccgatg atcataacta agcttcctaa taaaagccct aatccccaca
    33241 tacgagtaga agatttgctg aaagaaccag caacatatcc ttgtaacccg tggatcacta
    33301 atgaaaaaac tgcccactgc ggatatcctg aaatcaaatc gatcaataga ccactggaag
    33361 caccaacaac taatccaccc gttggaccat aaagaaaagc agcggtatag atcccggcct
    33421 cgcataatgt cacaaatcca ttcgtagccg gtacagggat caaaattgtt aatgaaaggg
    33481 caactgtcaa cgcaatcagc atagcataga tcgttaattg cttagttttc atttctcatt
    33541 cctccttttt gaataccgcc agaccagaca cttccggtat gatcgttcat cagcacgcca
    33601 ttagaaatac aatgggcaac aaatttttta ctttctgcta ctgctttggt aaaatctgcc
    33661 cctaacacaa gattcgctgt tattgcagaa gaaaaggcac aaccggcccc gtttactgtt
    33721 gtagtgtcga tttttttatg agaaaatacc tcataatgat caattgaata gagaagatcc
    33781 acagcttcat ctccagccat ccttgtgccg cctttgatca caatgttttt tgttcctgaa
    33841 gcagtgagtt ctttagcgag atggatcatt tctgttaacg aagagggaag agcttgacca
    33901 gaaagtaaag cagcttctgc caaattaggc gtaatcatcg taacaagtgg aaaaagttct
    33961 tcgatgagct gcgaaatata caattgattg atcacagctt cagtttcttt gaatgccaaa
    34021 acagggtcta aaatgattgg gatattttgt tttgttttta tgaagtctcg aacaaccatg
    34081 attgcttcac ttgaattcaa taacccaatt ttgataccat ctaatgaagt cattttatca
    34141 atcgaaacta actgtcgttt aagcaattcc aaagatatag attccagcat aaatcgctca
    34201 ttaatatcta gtgcccctat agaagtcaag gcgcttagtc cgaatagacc aaaattctca
    34261 aaagttttta aatctgtttg gattcctcca ccagcaaatg gatcagaacc gccaattgtc
    34321 aagattcttt tcataacatt ttcctccatg aataagagta taacgaaatt ttcaaaaaag
    34381 aaaaacagcc aattggcata tttttatctc atccaattta aaaaaacaaa aagaaaagaa
    34441 agaccaaaat ggatctttct ttccttaaat agttaggact tttgtgattt taattctttc
    34501 tcgatttgaa gccgttgcac tttcattgta gctgttcgcg ggattgcatc aaaatcacgg
    34561 atgataggga cgttcaagcg aggaagttca tggacttgct cccaccatcg attccaatcc
    34621 atttctttat caggagctaa agcaataatc ggttgtggag aatgattttt atcccttaca
    34681 atcactactt ctgccaaaaa atctaacgaa tctaataaaa agtcttcaat agctaatgta
    34741 ctattgatcg tttcgatcaa atccacttgc cgatctttca aaaataggtg gccatgctta
    34801 tccataaaac cgtaatctcc gctatcccac caatcgccat aaacattttc ctggaaccgt
    34861 gcatcttcct tgtaataagt caaagcccgt cctttagaca gtaattgtat atgaccatct
    34921 gtgttttcag gtaggacatt cccaactgaa tctgtaatcc gagcgcttgt catgtcttcc
    34981 agtccaacgc ccatatctct tgcatcactt gttttcaaac tctctagtgt atgagcctta
    35041 agaatcattg gtccgcattc actttggcca tatacttgaa gaaataccgc atcctgtgcc
    35101 atcgacgttc gcaaaaatgt cttcattgtc tggttgttta tagcatcaaa agtggagtga
    35161 tagtatcgga tacccgaaaa agcttctgga tgctccttag cagtaaaacg ccactgaaca
    35221 aagttattcg gatgggtctc tagtgcaatt ggccggtgag cttccaacat atgaacaact
    35281 tttgaacttt gtgcgtttgc taaaggcatc ataggaaatc ccatggacat caaagaagag
    35341 ataccaatat tgaatctgga atgcactgga gagatgtgaa aagcgattaa tttcttttcc
    35401 gaaatttttg tgaaaactgt tttttgccat ttcgtccgcc atcccataga atgagcagaa
    35461 tgacaaataa gtttagggat accagttgta ccagaagtat gggtcatata agaaatttca
    35521 tttttgtcta aaaatgattg agaaacagac aaagcaggtt gttccattaa ataaagaata
    35581 gga
//
LOCUS       DAYYGO010000013        35156 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000013 DAYYGO010000000
VERSION     DAYYGO010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35156
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.013"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(267..635)
                     /locus_tag="ACHA5T_000579"
     CDS             complement(267..635)
                     /locus_tag="ACHA5T_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFM8886183.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            785..1267
                     /locus_tag="ACHA5T_000580"
     CDS             785..1267
                     /locus_tag="ACHA5T_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFM8886184.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            1304..2314
                     /locus_tag="ACHA5T_000581"
     CDS             1304..2314
                     /locus_tag="ACHA5T_000581"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFM8886185.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(2429..3142)
                     /locus_tag="ACHA5T_000582"
     CDS             complement(2429..3142)
                     /locus_tag="ACHA5T_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886186.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(3315..3922)
                     /locus_tag="ACHA5T_000583"
                     /pseudo
     CDS             complement(3315..3922)
                     /locus_tag="ACHA5T_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            4406..4633
                     /locus_tag="ACHA5T_000584"
     CDS             4406..4633
                     /locus_tag="ACHA5T_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFM8886187.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            4649..5815
                     /gene="hemW"
                     /locus_tag="ACHA5T_000585"
     CDS             4649..5815
                     /gene="hemW"
                     /locus_tag="ACHA5T_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFM8886188.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNAHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            6049..7092
                     /gene="hrcA"
                     /locus_tag="ACHA5T_000586"
     CDS             6049..7092
                     /gene="hrcA"
                     /locus_tag="ACHA5T_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFM8886189.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            7186..7749
                     /gene="grpE"
                     /locus_tag="ACHA5T_000587"
     CDS             7186..7749
                     /gene="grpE"
                     /locus_tag="ACHA5T_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFM8886190.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            7800..9629
                     /gene="dnaK"
                     /locus_tag="ACHA5T_000588"
     CDS             7800..9629
                     /gene="dnaK"
                     /locus_tag="ACHA5T_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFM8886191.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            9780..10946
                     /gene="dnaJ"
                     /locus_tag="ACHA5T_000589"
     CDS             9780..10946
                     /gene="dnaJ"
                     /locus_tag="ACHA5T_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFM8886192.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(11028..11657)
                     /locus_tag="ACHA5T_000590"
     CDS             complement(11028..11657)
                     /locus_tag="ACHA5T_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8886193.1"
                     /translation="MGQLSDNQQLCQERINPLLELLERVFSYYGQALLTVHRQQIIIL
                     VNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSFLHAKQVCEWIAF
                     HQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLFKKTLACFSKNNG
                     SIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLLVQT"
     gene            11669..11842
                     /locus_tag="ACHA5T_000591"
     CDS             11669..11842
                     /locus_tag="ACHA5T_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886194.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(11887..12624)
                     /locus_tag="ACHA5T_000592"
     CDS             complement(11887..12624)
                     /locus_tag="ACHA5T_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8886195.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(12626..14128)
                     /locus_tag="ACHA5T_000593"
     CDS             complement(12626..14128)
                     /locus_tag="ACHA5T_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFM8886196.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            14387..16381
                     /gene="uvrB"
                     /locus_tag="ACHA5T_000594"
     CDS             14387..16381
                     /gene="uvrB"
                     /locus_tag="ACHA5T_000594"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFM8886197.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            16391..19207
                     /gene="uvrA"
                     /locus_tag="ACHA5T_000595"
     CDS             16391..19207
                     /gene="uvrA"
                     /locus_tag="ACHA5T_000595"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFM8886198.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            19470..20735
                     /locus_tag="ACHA5T_000596"
     CDS             19470..20735
                     /locus_tag="ACHA5T_000596"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFM8886199.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            20739..21470
                     /locus_tag="ACHA5T_000597"
     CDS             20739..21470
                     /locus_tag="ACHA5T_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFM8886200.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            21605..22489
                     /gene="rapZ"
                     /locus_tag="ACHA5T_000598"
     CDS             21605..22489
                     /gene="rapZ"
                     /locus_tag="ACHA5T_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFM8886201.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            22486..23484
                     /gene="yvcK"
                     /locus_tag="ACHA5T_000599"
     CDS             22486..23484
                     /gene="yvcK"
                     /locus_tag="ACHA5T_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFM8886202.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            23518..24453
                     /gene="whiA"
                     /locus_tag="ACHA5T_000600"
     CDS             23518..24453
                     /gene="whiA"
                     /locus_tag="ACHA5T_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFM8886203.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            25021..25677
                     /locus_tag="ACHA5T_000601"
     CDS             25021..25677
                     /locus_tag="ACHA5T_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886204.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            25856..26421
                     /locus_tag="ACHA5T_000602"
                     /pseudo
     CDS             25856..26421
                     /locus_tag="ACHA5T_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(27120..27641)
                     /locus_tag="ACHA5T_000603"
     CDS             complement(27120..27641)
                     /locus_tag="ACHA5T_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFM8886205.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            27839..31519
                     /gene="nifJ"
                     /locus_tag="ACHA5T_000604"
     CDS             27839..31519
                     /gene="nifJ"
                     /locus_tag="ACHA5T_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFM8886206.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            31708..32595
                     /gene="cdaA"
                     /locus_tag="ACHA5T_000605"
     CDS             31708..32595
                     /gene="cdaA"
                     /locus_tag="ACHA5T_000605"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFM8886207.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            32592..33761
                     /locus_tag="ACHA5T_000606"
     CDS             32592..33761
                     /locus_tag="ACHA5T_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFM8886208.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            33785..35140
                     /gene="glmM"
                     /locus_tag="ACHA5T_000607"
     CDS             33785..35140
                     /gene="glmM"
                     /locus_tag="ACHA5T_000607"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFM8886209.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
BASE COUNT        11575 a         6032 c         7613 g         9936 t
ORIGIN      
        1 gaaagatcac tgatattttc tcgtattttt tccttattca aaagttttgc aggaaaattg
       61 ctgaaaaaat aattttacta agtagaaaat tcaaagccat tcaatcagtg atttttttca
      121 gcacaagaaa gactccttat aaaaatagca gatactttgt caattaagtg tttaaccagg
      181 gatgataaaa aggataggat tgaacaaaac ctgtataagg tgatgaatag tttcctcgcc
      241 agtaaaaggt ttattcgtat aaagctttat cgccaaaatt tatcatgacc ttttttatct
      301 tcaatattaa atgaaatcaa ttgttcaatc aattcccagt tgattacact tttccaagga
      361 actttgatga cgttatccgt atatacgtag cctgcttctt ggatattttt ttcaagggca
      421 cgaatcgctg ctttttccgg tgcgatagaa aaatgatttt tagcaaaact aaatgcaata
      481 ataaaggtac catggtcagt aaacataggt tggttccatt ttacagttgt gtctagctgg
      541 ggatacgttt ctgttatctt agcaaaaagg tctttgatct tttgttgatg ttctttttct
      601 ttgatttgat tgatgatcgg ctgaaatttt tccattctaa aacgcctcca ttaagtggtt
      661 tgatttattt taccagaaaa acaatagtta tgtataaaaa acgaacaaaa cacaagtaat
      721 gttgtaaccg ctatatttat atgataagat gtctcaaata ataaaataga aaagagacat
      781 aaaaatgatt gacttggcaa agaaattctt acacgaaaat atatattttg agttgtatca
      841 tcatcgagcc atttatacaa atgaagatgc tttggtaata aagaaagaac aaggatttac
      901 aggtacagag accaaaagct tattcttaaa agataaaaaa gataataact acgtatatct
      961 gacttttact acgaaacaaa cggatttcaa agcattaggg aagatcgtcg gtaaaaggct
     1021 ttctattgtt tcatcagaac gcatggaaga acaaacggga caaaagcagg gcgctgtttc
     1081 tccgtttggt tatgaaaaac agattccgat tattattgat gaagagctct tatcaaatga
     1141 aaaacttgtt tttgcaccag gtagaccaga tcaaacgatg gtcatccaaa cggttgactt
     1201 aaacaaaatc atccagctac ttgagatgcc gatatttttg atgccggcaa acgaaaaaaa
     1261 caattaacca agtaaaatag tgcatacttg gaggtgaagc aacatgataa gttatccatt
     1321 attagaaaga aatgcaacga ttggggttac tgcaccatcc tcaggcgtat cagaggaaca
     1381 ccattcgtta ctcgaatcag ctttaaagag aatggaagaa aaatctttcc aagtagttac
     1441 tggaaagacg ctttggacgc aagataagga aaaatcaggt ccagcaagaa aacgagcaga
     1501 tgaattcaat caaatgatgc aagatgaaaa tatccaaatg atctttccga cttggggagg
     1561 agaattattg attgagattc tcgattttgt cgattttgat gctctacaac caaaatggat
     1621 agtaggttat tcagatatta gtacgttgct acttgctgtc acattaaaaa cagggatagc
     1681 cactgcacat ggaactaacc ttattgattt aagaggagag tacttggatg aaacgacagc
     1741 aatgtgggaa agtgtactga aaacaagaga aggagaatcg attctacagc aatcttctga
     1801 aaagtatcaa aaagtgtggc cccatgagca ttcatcatct tctgttttcc atctcactga
     1861 accaacaaat tggcaatcag tcagcggaga tgcagtaagg atgaagggac gattattagg
     1921 tggttgtatt gatacgatca ggcatttaat cggtactcct tatggagatg tccaatcttt
     1981 taaaagaaag tataatgatg gaaagccgat cttgtggttt ctagaaaact gtgagctatc
     2041 gtcagttgat ttaagaagat cacttgttca gatgaaactg gcaggctggt ttgatggcag
     2101 ctcggggatc atgttcggta gaagtgcggc agaggatttg ttagattata aaatgaagga
     2161 tgtatacgaa gatctgtcaa aggaattaaa actaccgatc ttgtatgata ttgattgtgg
     2221 tcatgtaccg ccacaactga cattgattaa tggtgcctat gcagaagtag cggtagaaga
     2281 tagaaaagcg actgttttgc aaacgtttat ttaaaaaatt ttttcctgcc ttggagtcta
     2341 tcaaagacca ataaatctaa atagaaaaga aagtgaaaga agctgacaaa atgctgtcag
     2401 cttctttttg tataacatat tagaaagtct aattatctat aactgtcaca tcgattaact
     2461 gtttcacgtc attgggattt cctgtaggaa tagctactac agatgcatta tcaatgattt
     2521 cttgagttgt ttctctaatg aacgctggat aagatgtttg atctgcaggc atttgccaat
     2581 gtgaaggaac tggataacta gtgattgtcc cgtcatggtt acttgaatac actaaaactc
     2641 cctggtaact gtatttccca gtgagcataa ccacatcttt cggatagact gcactccctg
     2701 catcataagg ggcgataggt gttcctgcag gataatgaat aacatgtaat tcgaaatcac
     2761 ccgatgtatc tctaaaagta ttacctctaa tagctagcca aacacgtgcg tactcaattt
     2821 gtagatcaga atatcctgct aacggatcta ttgactgaga agactgtgag gaggttgtgt
     2881 ctattgttga agaagaaata tttgtctcaa ctgtactact agatgtagaa gaagtattgg
     2941 gctctgagac tttactgctt gttgaagatg tattcgtttc agtagaagct gatttctgag
     3001 tacttgattt ttcagctgaa ctggtttgtc tgattgaact tttgtctgtg gtcgcagatg
     3061 gatcactatt ttctttacta ggagacgtac atcctacaag gaataaagag accaacaata
     3121 aaataaaacc taactttttc atataaaata cctcattatt tttttaatac cagtatagca
     3181 taatcctgaa tttttgaatc cgtaaaacct tgtctatgaa tataaataga gtgtatcttc
     3241 tttgataaat gtcagaaaaa gtgtgacaaa taatcatcag taaaattact tgtcacacta
     3301 ttgataagga gtatttaatt gaagagaatg attaggagaa aggatctccg tactttttat
     3361 agtaatcata gaatccaagt ttgtatagac gaatcgtttt ttctcttatt tcttctgttt
     3421 caaaatggac tgcttgaaga atcaagtctg tgaattctat aggagctgtt ccattcgcag
     3481 taatcaaatt acgatctttc acagcttgtt gttctataaa atcttcttta ttctgataat
     3541 tttccgattc taaccaagga aattgcgcat ttccggtgtg tttgtaacct tgcaataatc
     3601 cgctctgagc taaataatca actgccccac aaatagcagc tactggtacg tctgttttta
     3661 acaatttatc gattatttga tataaatgat gattttttat gtcccatgaa ttgccaccga
     3721 tcataataaa aaggtcaact ttttcaggta tttgttcgat taagtaatcg atttttgttt
     3781 ttaatcctcc aatggaagtg acctctgatt ggatagaagc agttttgttt tgccatttct
     3841 cattttggtt tagtgtgcta gtaatataag caccttccca atcggcatat tcctctaata
     3901 gaaacgtaat cgcagttttc attttcaagc tctcctttac tatctataac aaggacaaca
     3961 gttttaacct acagacattc attaaaattg ggtagcgaca gcgaaaacaa caattcttaa
     4021 gttttagaca gagtatacag atagtcaact taattaaata cgaactaaat aaaaatgaca
     4081 ggtaatcatt tttgtttacc agcattataa cattttgcat acatggatga caaaagaacc
     4141 aattcccact tgcttgagta caatattaaa agaaactgga aggcaaaaag taattataga
     4201 aacgaaaaat aaatcatatc gggcactcag acatgaaaac aaccctagag atatataact
     4261 aggtaaccag tttaatgaaa gaaaaaatca tccaagaagt cgattcttgg attttttagt
     4321 ggatttgttc gccaaatacg tttagtatgc gtatatgaga ggttgtaagc aaagcgttgt
     4381 ggtctgagca cttaaggagg aatctgtgaa aatagctttt catatttttg cagattttga
     4441 cttaatgccg aaggtcagct ttgcgattac cgtttatcga gaggctgtaa aagcaccgtg
     4501 tagctccgat gacaataagc aagcattttt agagctaggt ttccttgatt ctaaatatgc
     4561 ggcttatttc aatgagttgg tgcttgaacg cgattttaag aaggtcgaaa acaggcacga
     4621 cttttcgacg taaggagaaa gaatttcaat gatagaaaca ataaatcccg cagaaaacaa
     4681 ggtttctgcg tacattcata tcccattttg cgagcatatc tgctattact gtgattttaa
     4741 taaagttttt ttagaagggc agccagtaga tgagtatgtg gaaatgctgc tgaatgaaat
     4801 gaaaataatg ctagaaagtc atccgacagc gaatttagaa acactttacg tagggggcgg
     4861 gacaccaact tctttatctg ctaaacaact tgatcgactg ttatcaggtg ctagagagat
     4921 cctgcctttt caaaatggta atgagtttac tgtagaagcg aatccaggtg atttgacccg
     4981 tgaaaaactt caagtaatga aaaactatgg cgtcaatcgt ttatccatgg gagtgcagac
     5041 atttgataat cgcttattga aaaaaattgg acgtaaacac accgcagaag atgtgtatca
     5101 aactatgcaa tttttagaag cagaaaattt taccaatgta agcatcgatt tgatttatgc
     5161 attaccggga caaacactag aaggatatct ggatactttg gaaaaagctt tggctttgga
     5221 tctgcctcac tattctttgt actcattgat cctcgaaaac aaaacaatgt tcatgaactg
     5281 ggtacgacaa ggaagactcg aactgcctga tcaggaaacg gaaaccagaa tgtttgaaga
     5341 gacgattgaa gcaatggaaa agcatggacg tcaccaatat gaaataagta actttgggct
     5401 ctccggacat gaatctcaac ataacctcat gtactggaac aatgcccact attttgggtt
     5461 tggagctggt gctagcggtt atcttggaga tattcgttac cgcaacaaag gaccaatcca
     5521 acactattta cgcccattac gagcaaatga acgcccggta ttggaagaag aaaaattgac
     5581 acgaaaaaat caaatagaag aagaaatgtt cttaggacta agaaaaaaaa cagggattac
     5641 taaaaaacat ttctatgatc gctatcaaaa aacaattgaa tcggtgtacg gtgatgtgct
     5701 aagggaatta gagcaagaag ggttacttgt taatgaagaa gaccgtgttt atttgacacc
     5761 aagagggacc tttttgggga acaatgtgtt tgagcgcttt ttgtttgaaa aatagtttaa
     5821 agaatatttg ttttttatct gaatttaaat aaaaaaagtt caaaaataca ataaaggact
     5881 attgaaacgt tgatataact ggatttacat caattattta gaattagcac tcttgtgttt
     5941 agagtgctaa aaaatggagt ctttctcttg acaggagaag ggcttcttgc tatatttata
     6001 agtggattag cacttagaat atatgagtgc taatgagagg tgaggaagat gttaacaaag
     6061 cgacaaaacg acattttacg tctgatcatc caacactata cgagtagtgg cgtgcccgtt
     6121 ggttccaaaa cattgatgtc tgagggagtc aaggccagct cagcaacgat tcgcaatgat
     6181 atgaaagcat tagaagatga aggattgctg ttgaagactc actcttcttc tgggcggatt
     6241 ccatctgctt taggctatcg ttattacgtg gatcatttgt taaaacctgc tcgcgtagca
     6301 gaagatgatt tgctgctgat tcgtcagtct ctaggcagag aatttcatga aatcaatgag
     6361 attattaagc aatctgctga gatattatct gagctgacaa gctatacgac tttttcattg
     6421 ggacctgaaa taaaagaccg ccgactgact ggctttcgta tcgtaccctt gaataatcga
     6481 cagattattg ctatcgtcgt aacagatatg gggaatgtag aaagccaagt gttcactctt
     6541 cccgaatata tgggaagtca ggaactagaa aaaatggtac agattgtaaa cgaccgtcta
     6601 gtcggagatc ctctgatgac ggtgtaccat aaacttcgta cggagattcc gatgattctt
     6661 cataaatatt tccaaacgac agaagggatg tctcagttat ttgacaccgt tcttggtcat
     6721 gtttttgaag ataaggttta tgtcagtgga caaatgaatc tcttggatta tgagccccgt
     6781 caagatcttg atcagttcaa atcgatgttt tcttttatga aagactcaga cggacttaca
     6841 caaatgatca ctccgatgga tagcaatatt catatcaaga ttggtgctga attaggcaat
     6901 gatttattgc agaatatgag tatgattcaa gcaagttacg agatagatgg tcatggaaga
     6961 ggtacaatag ccttgttagg gcctactagt atgccttatt ctcggatgtt cggtctagtc
     7021 gatgtttatc gaagagaact agcagctaaa cttgctgatt actaccgttc gcttgacttg
     7081 tcaaactcgt aatgtgaata acgaagctat ttgcagctga tcgttctctc tttcttagta
     7141 agagcaatca aacagctgac tgaataatga aaggaagact gtatcgtgaa agaaaatcaa
     7201 gaagaactag ataaagaaat gacagatgct cagccagaac cggaaatcga tgtcgaagct
     7261 gcagaagatt cgggtatttc agaagctgaa gcggaagaat tcgaaacagc gaaattaaaa
     7321 gctgagttag aagaaatgga agataaatac ttgcgggctc gtgctgaaat cgcaaatatg
     7381 gcaaatcgag gaaaaaatga gcgtgagcaa ttacaaaaat accgttcaca agatttagcc
     7441 aaaaaattac ttccatcaat tgacaacctg gaacgtgcat tagccacaga agtgtcagat
     7501 gatcaagggg caggtttgaa aaaaggcgtg gaaatggtct tggaaagttt gcgtaacgca
     7561 ctggaagaag aaggaattga aaaaatccct gctaaaggag aagcctttga tcctaatctt
     7621 catcaagcag tacaaactgt tccagcgact gaagatacac cagccgatac gattgtagaa
     7681 gtactacaag aaggctacaa attacatgat cgcgtgttaa gaccaaccat ggtcattgtt
     7741 gcacaataat tgtgcggata taaaacttac actttcaatt aaagaaggag acgttaatta
     7801 tgagtaaaat tatcggtatt gacttaggta cgactaactc tgcagtagca gtattggaag
     7861 gaaacgaagc aaaaatcatc gctaacccag aaggaaaccg tacaacacct tctgttgtat
     7921 cattcaaaaa tggagaaatc caagtagggg aagttgcaaa acgtcaagct gtgacaaacc
     7981 caaatacgat ttcatcaatc aaacgccata tcggtgaagc aggttacaaa gtagatgtag
     8041 aaggtaaatc atatacacca caagaaatct cagcaatgat ccttcaatat atcaaaggat
     8101 tcgcagaaga ctatttaggt gaaaaagtcg aaaaagcagt tatcactgtg ccagcttact
     8161 ttaatgatgc acaacgtcaa gcaacaaaag atgctggtaa gatcgctggt ctagaagtag
     8221 aacgtatcgt caatgaacca actgcagctg ctcttgccta tggtttagat aaaacagatc
     8281 gcgacgaaaa aatcttagta ttcgaccttg gtggcggtac ttttgacgta tctatccttg
     8341 aactaggaga cggtgtcttt gatgtattgt caacagctgg cgacaaccat ctaggtgggg
     8401 acgactttga taacaaaatc atcgactaca tggtagcaga attcaaaaaa gaaaacagca
     8461 ttgacttgtc tcaagacaaa atggctttac aacgcttgaa agacgctgct gaaaaagcga
     8521 aaaaagattt atctggcgta tcaagcacac aaatcagctt gccatttatc acagcaggag
     8581 aatctggacc attgcatttg gaaatgacat tgacacgtgc aaaatttgat gaattaacag
     8641 ctgacttagt agaacgtaca aaagtaccag tacgtcaagc gctaaaagat gcaggtttat
     8701 cacaatcaga aatcgacgaa gttattttag tcggtggatc tactcgtatc cctgcagttg
     8761 tagaagcagt acgtaaagaa acaggaaaag aaccaaacaa atcagttaac cctgatgaag
     8821 tagttgctat gggtgctgct atccaaggtg gcgtgatcac tggtgatgtg aaagatgttg
     8881 ttttacttga cgttacacca ttgtcattag gtatcgaaac aatgggtgga gtgttcacta
     8941 aattgattga ccgcaataca acgattccaa caagtaaatc acaagtattc tcaactgcgg
     9001 cagataatca accagcagta gacatccatg tattacaagg tgaacgccca atggctgctg
     9061 acaacaaaac tctaggaaga ttccaattga cagatattcc agcagctcct cgtggtattc
     9121 cacaaatcga agtaacattc gatattgata aaaacgggat tgtaaatgta tctgcgaaag
     9181 atctaggtac acaaaaagaa caaaaaatca cgatcaaatc ttcttcaggt ctaacagacg
     9241 aagaaatcga acgtatggta aaagatgcag aagcaaatgc agaagcagat aaagctcgta
     9301 aagaggaagt tgacctacgt aatgatgtag atgcattgct attctctgtg gacaaaacat
     9361 tgaaagaatt agaaggcaaa gtggacgaag aagaagtgaa aaaagcagaa acagctcgcg
     9421 atgaattgaa agccgctgtt gaagcaaaca acatcgaaga aatgaaaact aaacgcgatg
     9481 cattgaacga aatcgttcaa aacttgactg ttaaactata cgaacaagcg gcacaacaac
     9541 aagctcaaga aaacccagaa gcagctcaag gcggagcaga tgatgtcgtt gatgctgatt
     9601 ttgaagaagt cgatggcgac gataaataat cagttcactg aatttcgaga gaaggccaaa
     9661 gttgctactt tggctttttc ttttgaaaaa tagtttgaag tatcggtttt tgtttcaaac
     9721 aaaatatgat atgataacaa gcgacgtgaa aatgatgaga accaatagga ggaaagccta
     9781 tggcaacaaa acgtgattat tatgaagtct tagggctctc aaaaggagca tcagaagatg
     9841 aaataaagaa agcttatcgt aagctttcga aaaaatacca tcctgacatc aataaagaac
     9901 ctgatgcgga agaaaaattc aaagaagtat cagaagcata tgagatttta agtgacccgc
     9961 aaaaacgcgc tgcatatgac caatacgggc atgcaggcac agatccaaat tacggtgctg
    10021 gtggagctgg tggcggattt ggtggattcg gcggcttctc tggtggcggc ttcggtggtt
    10081 ttgaagatat cttcgactct ttctttggag gaggcggtcg gtcagtcgat ccaaatgcgc
    10141 ctcgtcaagg tgctgatttg caatatacgg tcgacttgtc ttttgaagaa gcgatttttg
    10201 gtgtagaaaa agaaatcaag tataatcggg aagaaatctg tcatacttgt ggtgggaacg
    10261 gtgcaaaacc aggcacacaa ccaactactt gtcataaatg tcatggtgca ggtacgatta
    10321 atgtagaacg ccaaacacca cttggacgag tgatgagccg tcaaacttgt gatgtttgtc
    10381 atgggacagg gaaagaaatc aaagaacctt gtccaacctg tcatggctca ggacatgaga
    10441 agaaagcaca ttcagttaaa gtgaatgtac cagctggtgt agaagaagga caacaaatgc
    10501 gtttagctgg tcaaggagaa gctggagaaa acggcggacc atttggtgat ttgtatgtgg
    10561 tcttccgtgt agaagaaagt gatatctttg atcgtgaagg ttctgaaatt tactatgaat
    10621 tgcctttaag ttttgtacaa gcagctttgg gggatgaagt ccaagtacca actgtccatg
    10681 gagatgtgaa attgaagatc cctgctggaa cacagacagg tacgaacttc cgtttacgtg
    10741 gaaaaggcgc tccgaagtta cgtggtggtg caacaggcga ccaacatgtc aaagtaaaac
    10801 tgatcacacc gaaaaatcta aatgaacaac aaagagaagc attacgtgca ttcgctgaag
    10861 ctggcggcat caaagttgaa gaacagcaag aagacggttt cttcgataag tttaaagatg
    10921 cttttggcgg gaaaaagcgg aagtagtttt tctagtaaaa acgctaagtc attaataatt
    10981 aaaaaccaga cgaacaatca tatgttcgcc tggttttttt gtagttgtca tgtttgtacc
    11041 aacaaaaaag ccagctttag aatatgataa tcgtcatagt ttctaggggt ataaccagtg
    11101 agagaatgga acttgttcaa tcgatattgc aaggtgtttt tatgaataaa taattcttct
    11161 gagcaatttt tgatgcttcc gttattttta ctgaagcatg ccaaagtctt cttgaacaaa
    11221 tgtacttctt cttctgtcag gcttttcaaa atacgtttta catataatgt tcgttgatct
    11281 gacgggattg ctggtaaaac gatcccgagg tctaaatctt caaaaaagcg gatctcgttc
    11341 acagattgat ggaaagctat ccattcgcaa acttgtttgg catgaaggaa agattgcggt
    11401 gtctcttgct ctgtataaca aagcgaccca attccaaagt ttagctgtaa ctgatacatt
    11461 ttattaaact ttgtctggat tttatcaagc aatgatagca atgaggctct ggaaatccga
    11521 ttgactagga taatgatttg ttgtctatgt acagtgagca aagcctgtcc gtaataagag
    11581 aagacccttt cgagcaactc caataaagga ttgatccgtt cttgacacaa ttgctgattg
    11641 tcagaaagtt gtcccacaac tacataatat ggagaagaaa aatcaagctc aattaagtga
    11701 gggtggtatc gactatgtac ttgatatcat caatgaaccg atggtacttt cttatgcgat
    11761 ggaacaggta gagcgattgc ttgtttgtgc aggaaaacaa gcgatgcggc tgatacgatt
    11821 aggtgagaaa ataagaagat aaaaatacac ggtcccttca tggcgaccgt gcatttttgg
    11881 tttttattaa atatttaata ctttattcaa gaaatctttt gtccgttcat tttgcggatt
    11941 ttcaaagatt tgttgaggcg ttccatcttc taaaaagttt ccgtcgtcaa tgaacatgac
    12001 gcgattggcc acttctttag cgaaccccat ttcgtgcgta acgataacca tcgtcatacc
    12061 ttgttctgct aactttttca tgacgttcaa gacatcaccg accatttcag gatcgagtgc
    12121 agaagtaggc tcatcaaaaa gcatgatgtc agggttcatc gctagagcac gagcaatagc
    12181 cacacgctgt ttttgtccac cagaaagcat ttcaggatac atatcttttt tatcttctag
    12241 accaactgtc tgcagtaatt tgattgcttt tttctctgct tcttctctag attctttctt
    12301 caggtccatc ggtgctaaaa tgatgttttc caaaatagaa agatgaggga ataggttaaa
    12361 gtgttggaat accattccga tatgttgtct caccaagttg atatctgtgc ttttatccat
    12421 caagtgggcg ccgtcaataa taatttcacc gtcagaaggt tcttctaaac gattcaagca
    12481 acgcaagaat gtactttttc ctgaacctga agggccaatg atacagacga catctccttc
    12541 gttgatggat acattgatgt cgtttaatac tgtgttatcg ccgtattttt tgactaaatg
    12601 ctcaactaag attttttctg ccatcttact tcaccttctt ttctaaaact tttgctaatt
    12661 ttgtcaatgc tgtgatcaaa atcaagtaca tgatcgcaat gatcaagtaa acatacgtac
    12721 tttgtgttgt acgtgcaaca atgatcttac cagtttgcag caattcgact acaccgattg
    12781 cggaaatgat cgttgtgtct tttaatgaga taacgaactg attgacgaat gatgggatca
    12841 tgatgcgtat cgcttgcggc aaaatgattt tttgcattgt ccgattataa gaaagaccta
    12901 aacttctgga agcttccatt tgtccgacag gaacggcgtt gatcccgcct cgaacaatct
    12961 ctgcgatata agcacttgcg ttaagagtca aggtgataac accagcaagg aagtcaggaa
    13021 tcgtaaatcc aacgatacca ggcaacccaa agaaaataaa gaacgcaaga accatcattg
    13081 gaattcctcg gatgatatcg acataaatac tagcaattgt gcgaagagcg cgtattggtg
    13141 ctacactgaa taatccaaaa atcacaccga ttactatagc caaagcaaag gagatcaaag
    13201 ttaggagaat tgttctccaa agtcctttca acaatgcttg atagttgttt tggataagtc
    13261 caagaaatgt tgattcatca gcagtggaag actcactgga agtttctgaa ccatccgcta
    13321 catattcttt aataatctta tcgtattcgc ctgttctttt taattctttc aatccttcat
    13381 tgaacatatt aagaagctca ggattttgtc cttttttaac ggcaaatgca tattttccgc
    13441 cagattcttt tttgatcggt gtttccaatt cttgtccttg tttgatcgca taaccgatta
    13501 ccggataatc atccatcatt gcatcgattg cgccaacttt taacccgtcg tacaattgat
    13561 cagcagcatc aaaatatttg atagaatagc catattcaga ttcatggtcg cttaaaaatt
    13621 cagcactttc ggtaccaact tttgctccga ctgtttttcc tttcaaatct tcataagact
    13681 tgatcgaagt atctcctttt ttgacagcta attggatccc gctatcaaag taagaatcag
    13741 aaaaatcata gcttttcttt cgttcatctg ttacagtcat tccggcaatc attccgtctg
    13801 cttgtcctga ttccaaagct tgcaaagcag cagagaaacc acggtagtcc attcggatgt
    13861 taaatccttg cagtttggcg atgttttcca tcaattttac atcgatgcct tcgtactcgc
    13921 cctttgcgtt ttgaaattca aatggagcaa aagcagagtc actggcaatt acgtatacgt
    13981 cttttttggg ctctattttt tgcattgtct cgcttgtgct tgactctgcc gttgtagtgt
    14041 tgtcagcttg tgcaacatga ccaaacgcaa gtgtcacaag tcctgtcatt aagaaaacaa
    14101 atgagtgaaa taaatgcttt cttttcataa tcaacctcct aaatttccaa tattttatta
    14161 ttataggcga aaagtgctga atcagacaag actgtaagct cgaaaaaatg aaaaaaatgc
    14221 gagtttctaa cattttatgt tataaaacat aacatgtata ataagataaa aaagtggcga
    14281 aagtatgttc ggtgtggtat aattttttag taaggaatgc ctttgctttc cttttctttt
    14341 tattggtcgt aaaatgaaaa ggaatgaatg aaaaggagga cgaagaatga ttgagagaga
    14401 tacctcccga cactttgagc ttgtctcgaa ataccaaccg gctggtgatc agcctgaagc
    14461 gattaaccaa ttagtggatg gcgtagtcgg cgggaaaaaa gcacaaatct tattaggtgc
    14521 gactggaaca gggaaaacat atacgatttc caatcttatt gagaaagtga ataaaccaac
    14581 actgattatt gcccataaca aaacattggc tggtcagctg tacggggaat tcaaggaatt
    14641 tttccctaac aatgctgttg aatattttgt ttcttattat gattattatc agcctgaagc
    14701 ttatgtgcct tcaagcgata cgtatatcga aaaagattca agtgtaaatg atgagatcga
    14761 caaattacga cattcggcta ctagttcatt attagaaaga aatgatgtga tcgtcattgc
    14821 atccgtttca tgtatttttg gattaggctc gccttttgag taccaaaaac aagtagtttc
    14881 gattcgacag ggagcagaac tagatcgcaa tcagttgatt cgtgacttag tcagtatcca
    14941 attcgaaaga aatgatatag attttcagcg cggtcgtttt cgagtgcgcg gtgatgtcgt
    15001 ggagattttt ccggcttcaa gagatgaacg tgccttacgt gtggagtttt ttggagatga
    15061 ggtcgaacgt atccgagaag tcaatgcact gacaggtgaa gttttaggag aaacggaaca
    15121 cgtagcaatc ttcccggcga ctcactttgt gacgaatgat gaacatatgg agcatgccgt
    15181 agcgaatatc aaagcggaat tagaacagcg tttaactgtc ttgcgaaatg aaaacaaact
    15241 tctggaagct caacgtttag aacagcgaac caattacgat attgaaatga tgttagaaat
    15301 gggctacacg tcgggaattg aaaattattc tcggcatatg gacggacgta aagaaggtga
    15361 accgccttat acgctattgg atttctttcc tgaagatttt ttgatcgtgg cggacgaatc
    15421 gcacgtaacc atgcctcaaa tcagaggaat gtataatggt gaccgagcga gaaaacagat
    15481 gctagtagat tacggtttcc gtttgccatc tgctttagac aatcggccac ttcggttaga
    15541 agagtttgaa aaacatgtca atcagatcat ttatgtctct gcgacgccag gaccatatga
    15601 acatgaacaa acagataccg ttatccaaca aatcatccgt ccgacagggc tgcttgaccc
    15661 agtgatcgaa gttcgtccaa tcatgggaca aatcgatgat ctagtaggcg aaataaatga
    15721 acgagtcgaa aaagaccagc gggtgtttgt aacgacattg acgaaaaaaa tggcagaaga
    15781 cctcacagat tatttcaaag aattagggat caaagtcaaa tatttgcata gtgatatcaa
    15841 aacgctggaa cgaacagaaa tcattcgaga tcttcgatta ggtgaatttg atgtgttagt
    15901 tggaatcaat ttgcttcgtg aaggattaga cgtacctgaa gtttcccttg ttgctatctt
    15961 ggatgcggat aaagaaggct ttttacgtag tgaacgttcc ttagtccaaa cgattggacg
    16021 agctgctcgg aatgaagaag gtaaagtaat catgtacgca gacaaagtaa cagattctat
    16081 gcgtctagcg atggatgaaa cgtcaagacg tcgtacgatt cagcagaagt acaatgaaga
    16141 acatgggatc gttccaaaaa cgatcattaa agaaatccga gatttgattt ctattacgaa
    16201 agaatcggaa gatgatacaa aagaagcggt acaagtctcg tatgaagaaa tgacgaaaga
    16261 agaaaaagat acgttattga tgaaattaga aaaagaaatg aaaaatgcgg cgaaagcatt
    16321 ggattttgaa acagccgcta atgtgcgaga tatgatttta gaattaaaag cttcaaagta
    16381 ggaggacaac atggctaacg ataaaattac gattcacggt gcacgtgccc ataacttgaa
    16441 aaatatagat gtaacgattc cacgagataa attcgtggtc gttacaggtt tgtccggttc
    16501 tggaaaaagt tccctagcat ttgatacgct gtacgctgaa gggcagcgga gatacgtaga
    16561 gagcctttct gcttatgctc gtcagttttt aggacaaatg gataagccgg acgtagatag
    16621 catcgacgga ttgagtccgg cgatttctat tgatcagaaa acaacaagta aaaatcctcg
    16681 gtcgacagta ggtactgtga cggaaatcaa tgactattta cgcttgttgt ttgcacgtgt
    16741 aggacatccg atttgtccca acgatcatat cgagattacc agtcaatcag tagatcaaat
    16801 ggcagatcaa gtaatggctt tgcctgagcg cacaaagatc caagtgttgg caccgatcgt
    16861 ggtaaagaaa aaaggacaac ataaaaaaat attcgaacga atccaaaaag agggatacgt
    16921 ccgcgttcga gtcgatggtg agatctatga tttaagtgaa gtgcctgagc tggagaaaaa
    16981 caaaaaacat gatatcgcga tcgtcatcga ccggatcatt gtgaaagaag ggattcgttc
    17041 tcgtctattt gattctttag aggcggctct gcgattggca gacggctatg ctctggttga
    17101 cgtgatcggt gaagaagaaa tgttgtttag cgaacactat gcctgtcctt attgcggatt
    17161 tactgttggc gaactagaac caagactgtt ctcattcaat gcgccttttg gagcttgtcc
    17221 agactgtgat ggtttaggtg tcaaattaga agtcgatcaa gatttagtga ttccagatag
    17281 aacgaagaca cttagtcaag gtgctatcgt tccatggaat ccaatcagct ctcagtatta
    17341 tcctcaaatg ttagcgcagg cttgtgaaag tttcggtatc gatttagata caccattcga
    17401 agaattaccg gaagagcatc aggatatcat cttgaatggg acagatgatc ttttccattt
    17461 ccattatgaa aatgattttg gtggcgttcg cgatgtagaa acgactttcg aaggggtatt
    17521 gaagaatatc gagcgtcgtt atcatgatac gaacagtgat ttcacgcgtg agcaaatgcg
    17581 tctttatatg acagaattaa cttgtcagac atgtaaaggt tatcgattga atccccaagc
    17641 tctatctgtt caaatcaatg gaacgaatat cgggcaaaca aatgagttgt ctattcaaaa
    17701 agcagtagat ttcttctcaa acttaacgtt atctgaacaa gagaaagtaa ttgccaaacc
    17761 gattttaaaa gaagtaaatg acagactttc attcttggaa aatgtgggac tagactatct
    17821 aactttgagt cgcgcttccg gaacattatc tgggggagaa gcgcagcgaa tcagattggc
    17881 tacacaaatt ggatcgaatc tttctggagt tttgtatatt ttagatgaac catcgatcgg
    17941 attgcatcaa cgcgacaacg atcgactgat tggctcatta aagaaaatgc gtgatcttgg
    18001 caacaccctc gttgtagtcg aacatgatga agatacgatg tgggcagcag attatctgat
    18061 tgacgtcgga cctggggccg gagaacaagg cggacaaatc gtggctgcag gcactcctga
    18121 agaagtagct aatgatgaaa attctttgac agggaaatat ctttcaggga aaaaatcaat
    18181 ccctgtacca aaagaacgcc gtaaaggtaa tgggaaagca atcaagatca ctggtgcttc
    18241 agaaaacaat ctgaaaaata ttagtgttga atttccatta ggagaatttg tagcagtcac
    18301 aggtgtgtct ggttctggaa agagtacatt ggtcaatagt atcttaaaga aatcgttagc
    18361 gcaaaaatta aataagaatt ctgctaagcc aggtaaattc aagacaattt ccggctacga
    18421 aagtatcgaa aagatcatcg atatcgatca aagcccaatc ggccggacgc cgagaagtaa
    18481 tccagcgact tatacaagtg tatttgatga tatccgtggg ttatttgctc aaacgaacga
    18541 ggcaaaaatg cggggttata agaaagggcg ttttagtttc aacgtaaaag gcggtcgttg
    18601 tgaagcttgt cgcggggatg gaattattaa gatcgaaatg cactttttgc ctgatgtcta
    18661 tgttccttgt gaagtatgtc atggcaaacg atataactct gaaacattag aagtgcatta
    18721 caaaggaaaa agcattgctg atattttgga aatgacagta gaagatgctg tagaattctt
    18781 caagcacatt ccaaagattc atcgcaaact gcaaacgatt gttgatgttg gcttaggtta
    18841 tgtgacgatg gggcaaccag caacgacatt gtccggtggt gaggcacaac ggatgaaact
    18901 tgccagtgaa ttgcacaaaa tctctaatgg aaagaatttc tatatactag atgaaccaac
    18961 gacaggactt catagcgatg acatcgcccg cttgttgcat gtattacaaa gattagtaga
    19021 tgctggtaac acagttttag tgattgaaca caatctagat gtaatcaaaa cagcagatta
    19081 tatcattgat ttaggaccag aaggtggaga aggtggagga acgatccttg cgactggaac
    19141 accagaagaa atcattaacg taaaagaaag ttatacaggt cactatttga aaaaaataat
    19201 ggtataaaga ggatagatag ctgagatagt taaaaaaaca taaaaaaatg aatttttgct
    19261 tgttttatta atatcagtgt tctagaatca ttacatggta gttctttcta cactgattgg
    19321 tatagacaaa aacttcctat aagttcaatc cgttcaaaaa agaattgagc aatcggagga
    19381 gtttcctttg attgcttttt tctttatggt atgatattcc ttgtgtagtt ggaaaagaaa
    19441 aacaagagat tatgagtaag tgagagataa tgatgaacag tattgaaaat gaagaattta
    19501 tccctatatt attaggaagc gacatgaacg tttatggtat ggcacgttct tttaatgaag
    19561 catacggcaa aatctgccaa gcatatgctt cggatcagtt agcccctacc cgctacagta
    19621 aaattgtcaa tgtagaagtt attccaggat ttgataaaga ccctgtgttt atcgagacga
    19681 tgcttcgttt ggcaaaagaa agatattctg ataagagtaa aaaatatctt ttgattgctt
    19741 gtggtgatgg atatgcagaa ttgatctccc aacataaaca agagctgtca gaatatttta
    19801 tctgtccata tattgattat tctttatttg aaagactgat caataaagta agcttttatg
    19861 aagtttgtga agaatatgat cttccgtatc ctaaaacatt gatcgtcaga gaagaaatgt
    19921 tagtaaatgg acatttggaa caggaattgc cttttgaatt cccagttgct ttgaaacctg
    19981 caaacagtgt ggagtatctt tctgtccaat ttgaaggaag aaaaaaagct tttattttag
    20041 agactagaga agagttcgat ttaattttag gccggatata cgaagccggt tataaaagtg
    20101 aaatgattgt acaagatttt atcccgggtg atgatagtaa tatgcgtgtg ctaaatgcgt
    20161 atgtagatga agatcatcaa gttcggatga tgtgtcttgg tcatccactg ttagaagatc
    20221 ctacaccagc atcgattggt aattacgttg tgatcatgcc agattataac gaaaaaatct
    20281 atcaaaccat taaggcattt ttggaaaaaa tcgaatatac aggctttgcc aatttcgata
    20341 tgaagtatga tcctagagat ggagaatata aattatttga aatcaatttg agacaaggtc
    20401 gtagcagttt ctttgtgaca ttgaacggct tgaatctggc tcgtttcgtc acagaagatc
    20461 gagtgtttaa caaacccttt gttgaaacga cctatggaac gaatcaatca gataaagcaa
    20521 gattatggat gggtgtgccc aaaaaaatct ttttagaata tgctcgggaa aatgaagaca
    20581 aaaaactagc tgagcaaatg atcaaagaaa atcgttacgg tactactgtg ttttatgaaa
    20641 aagaccggtc aatcaagcgc tggttgttga tgaaatatat gttccataac tatattccaa
    20701 gattcaaaaa gtattttcat gtgaaagaag gctagttcat ggagaatttt ttcagtattt
    20761 taggcggaat gggcacgatg gcgacagaaa gttttgttcg cttgatcaac caccgaacga
    20821 aagctacaaa agatcaagaa tatttgaatt atgtcttatt caatcatgca acagttcctg
    20881 atcgtacagc ttatatttta gatcgatcag aagaaaatcc aatgccgttt ctgctggatg
    20941 atattgagaa acaaaattta ttgcagccga acttcattgt gttgacttgt aacaccgcac
    21001 actacttttt tgaagaatta caagcggcaa cggatattcc tatccttcat atgccaagag
    21061 aagcagcaaa tgaacttgtc cgtcagcata cgacagggag agtagcgatt ctaggaacag
    21121 aaggaagtat gaaagctgga atctatgaac gcgaagtcaa aaatcttgga ttcgaaacag
    21181 tgattcctga tactgttctt caagaaaaaa tcaattatct gatctatcat gagatcaagg
    21241 aatcggatta tttgaatcaa gagctatatt acgaaatctt agaagaagcg gtagagcgcc
    21301 taaactgtga aaaagttatc ttaggttgca cagaattgtc attgatgcat gaatttgctg
    21361 aagataacca ttatccagtg atcgatgccc aatctatttt agcagatcgg acgattgaac
    21421 gagcgctagc tgaaagaagc aaggcattgg atacagcatc ggaaaagtaa aaaaattgga
    21481 tttggggcaa atatcctaaa agatatttgg tccaaatctt tttgtaaaag aaaaagaaaa
    21541 ctggtaatga tacgcccgga atgatataat tttactatgc acaatgtgag aggagttcgt
    21601 aattatgaca gataatttgc aattagtcat cataacaggt atgagcggtg cagggaaaac
    21661 agtagctatc caaagttttg aagatatggg ctatttttgt atcgataata tgccgcctcg
    21721 attgattcca aaattctggg aattaatcaa agaatcaggc aaagtcacaa aaatggcttt
    21781 aggagtggat ttacgttctc gttccttctt tgaagaaatc caaaacatgc tgatcgaaat
    21841 cgaaaatacg aacttcatcg acacttctat cttattttta gatgcttcag acgaagaact
    21901 agtggctcgc tacaaagaaa caagacgagc acatccaatg gcgatggacg gtttgatcac
    21961 tgaagggata cgtaaagaac gggcgatact agacgacttg aaagcccaag cttccgtgat
    22021 cattgatacg acgacattga caccacgaca attacgtgaa aaaatcaatg agtcattcaa
    22081 atcaaaggga gaccaaggat ttcggatcga atttgtatca tttggtttta aatacgggtt
    22141 gccgatcgat tcggatattg tgatggatgt gcgttttttg cccaaccctc attatatccc
    22201 ggaactaaga ccattgaccg gaaaagatca gccagtctat gattatgtga tgtcctttga
    22261 tgaaacgcaa tcattctacc ataaattttt ggatcttctg gaaactgtat tgcctggata
    22321 cattaaagaa ggaaaaagca gcttgacagt tgcaatcggc tgtacaggcg gtcaacaccg
    22381 ttctgtggca ttgactgaac gtttggcaaa tgctttaggg aaaaactata aagtcaatgt
    22441 gacacatcgg gacaaggata aaaggaaaga gacggtgaac cggtcgtgaa aatgaaaacc
    22501 taccggataa gaaaaccaaa gatcgtagtg gtcggcggtg gcactggtct gccagttatc
    22561 ctgaaaagtt tgcgaaatca aggagcagat atcacagcag tagtgactgt tgcagatgat
    22621 gggggaagca gcggtgcaat cagagaatcg atcgctatgg ctcctcctgg tgacttgaga
    22681 aatgtcttag tggcgttatc ggatatgcca caattctatg aagatatttt ccaataccgt
    22741 ttcaaaaagg aagatcagtt tttagccaat catacgatcg gtaatctgat cattgccgca
    22801 gtttccgaga tgcggggaag cacttatgag gcgattcaat tattagcaaa aatgatgcat
    22861 gtagatggac atgtttatcc ttcttcagaa actcctttga ccttgcatgc tgtctttaaa
    22921 gacggaacga ctgcagtagg agaatctaaa atcgcgatcg accgtaaaac cattgaccgt
    22981 gtgtatgtct gcaacacaaa tgataaaagt gaagccaaag cggctcgaaa agtcgtttca
    23041 gcaattatgg acgccgacat ggtcgttttg ggacccggaa gtttattcac cagtatcctt
    23101 ccaaatttag tgataccaga aatcggcgaa gcaatgctca agacaactgc tgaaaccgtc
    23161 tatatctgta atatcatgac ccaaaaaggg gaaacggaac atttcacaga tgccgatcat
    23221 atacgtgttt tacacaagca tttgaatcag ccttttatcg atacagtatt ggtcaatacg
    23281 gaaaaagtgc ctgaagatta tatggaccca gagatctatg atgagtatct agtacaagtc
    23341 gcacacgact tcaacggatt gagaaatgaa ggatgtcgag tgatttccac cgactttttg
    23401 gaattgaaag atggtggtgt cttccacgat ggagacaaag tagtagaaga attgttccgt
    23461 ttagttttcg gttcaaaata ttaagcaatt tattgttgga gaaaggagga gaataggatg
    23521 tcatttgctg cagatgtgaa gaaagaacta actggtttgg aagttcatcg agaacatgcg
    23581 aaagcggaat tagctgcttt gctgcgaatg aacggctctt taggtattgt gaaccagcag
    23641 tttattttga acgtccaaac agaaaatgct gcaattgcca gacgtatcta ttctcttttg
    23701 aaagatcatt atcaggtacg aagtgagcta ctcgttcgta aaaaaatgaa attaaaaaag
    23761 aacaatgtct atatcgtccg gttgaagcaa ggcacaaaag atgtgttgat ggatcttgat
    23821 atcatggatg gtatgatgtt caatggacac gtttcggacg agattatggg aaattcgcaa
    23881 aaaatgcgtt cttacttacg tggtgctttt atggcctcag gttcagtcaa taatccggaa
    23941 acgagccgtt atcatttgga aatattttct atttatgaac agcataacca agacatttgc
    24001 gatatgttaa attattacgg tctacatgct cgaacgcttg aacggcgcaa tggctatatt
    24061 tcttatttaa aaggagcaga aaaaattgct gatttcttaa cattgattgg tgcaaccaac
    24121 tcaatgctcc gatttgaaga tgttcgaatc gtacgggata tgagaaattc ggtcaatcgt
    24181 ctagtcaatt gtgagacagc aaatctgaac aagacgattg atgcggcttc taaacaaatc
    24241 gaaaacatcg aattgatcga agcaaaggtc ggtctacatg cccttcctga aaaacttcag
    24301 gaaatcgcag aacttcggct tcaacatcca gaggtcagct tgaaagaatt aggggaaatg
    24361 attccctcag gtgcgatatc aaaatcggga attaatcacc gaattcgcaa aataaatgaa
    24421 tttgcggaaa aactgcgtga agaagcagtt taatccaaag aaaaaactga tacatgaagc
    24481 aggagatgcg aatgtatcag ttttttttaa aagttatact tgcttttctt ccgtcaaact
    24541 ataattccat ctttactgga gtaacataat ttggaaaggc ttctattgtt tttaatacaa
    24601 tcgtataaaa agcggggaga caactgaatg aagattttag ttttgtacat aaatttttgc
    24661 ttaatataga agtcctgata tagataagat caagtattta tctataactg gcctgattaa
    24721 ttaaaaaact ggaaggaact gctcgatcat ccttgagaac tgctcttctt acttctggtc
    24781 aagattctcg tggttggtgg ataaggatgg ttgataagtt cattggatta gctagtcgaa
    24841 aacaaccagg tgtaattaat gtagaagttt taacaagggg atggagtgat ttattcagtg
    24901 ataatcataa gttcataaat agtaattgag atgtatggtt ggaatacgtt atacctgaac
    24961 ttgaactacc aagatttggt ttagaatttg agaatcgatg tttataaagg gagggcaaaa
    25021 atgcgttttt ttacaacttt tactatcatt atgattgcag tactttttat ttttctagat
    25081 atagcaaagc gaaatacagc atttttatta tatcgggttt tacttcgagc aggtctcata
    25141 acatttatat cgatcgttgg attttttcta ttcacagtta ttgtatttat ttggagaact
    25201 ccagcaccac cattgccaga gattacatat ggagaatttc cttttagact tgagtatgaa
    25261 ttgaatgaag aattacatgt gatagaagac acgttaatag ttgaatttga tggatttgga
    25321 atgaacgaag gcatagggag atatcgtaga tggacaagtc gtttagccag cggagaagat
    25381 ttagtgttgc tgttagaagt tagtgataat aagcaaatat tttattttcc aggacctgct
    25441 aattattaca tgggagatag gttgaatggt tataatcata cttttcctag tgcttctttc
    25501 attgaaaggg aaagaggcat tactagaagg gatattttac atgacaaaga attattagaa
    25561 caatttggtc cgcttgatca aaatacaata aatgaagaag aattgttaaa tcaatataat
    25621 ataaggctag tgaattggga aatatctgaa ccgattgtta acaattttgg tgattagaaa
    25681 acaattacga taaatagaaa caagccaaat catcagcaag taaaagctga tgatttggcc
    25741 tttcttatac gagtagtagg aattcatagc gaatattatt acgatcagta gatattttga
    25801 tattaaagga gtattacgaa ctagtatgtg agaatgtaga ggtgatttgc gaataatgag
    25861 aaatttatca aatagaaaca aaatattaat cattattgtt gttatagctg tgtttcatct
    25921 gggaacgaat gcggttttaa gtagaataat attgggtcct aagccaccaa gacctgcgat
    25981 tacccgaggg gagtttgatt ttagattaga atatgaagta gatggggaaa ggattgtgat
    26041 agaagataca attgttgcat tatttgatgg attttctgca gatgcaggta gcatggcatg
    26101 gtatcgcacg tggcgtttgc atttggcgag tgacaagagg gcacaacaat tattgttaca
    26161 tgagttagat gatggtcgaa aaatatatta tcattttgga ggagctggat tttatatggg
    26221 gaagttagta gcagtgtaac tgtaacaagg aaagatttgc caggtcttgg cattcgaaac
    26281 tccatatttt atatcgagcg tgggagagat acatctattg ggaacaggaa agggttaact
    26341 cttgaagagt tatataatga ctttggaatc cgtcttgtta gttgggaaca caatccacca
    26401 atagaaaatc gctttgaata atcgaaatct attaatccat ttcatcagcc agtgaatgtt
    26461 gtaatggaac aatttggttt agtgttcaaa accattgaaa tactaggctg tgatcagcaa
    26521 aaatgtatct gaataaaaaa gagagcaact attaaatgag aggagttagg agttgttagc
    26581 tttatacgaa tcaaagtggc aaatacagat gcgtatgcat ttgtgtttgc catcttgcgt
    26641 ttggtagacg agataggtat aagctaaaga aacaacttgt ttaaaataaa cgacctagct
    26701 caacaaacga ccatctaatc aactaacagc atacataaaa gcaactattt ttgaagtaga
    26761 attgaaatcg ggataactta aaaagtgatt gggatactag tgtgaatcac cgtacataaa
    26821 tattactcgt taagggaatt tgagaaattt tgtatgaaaa tttaagaatt agttagtatg
    26881 cgcaagtcaa aaatatgaat aaattatttg gtggagataa tttgctcagc ttgtcataag
    26941 aaatgagata atctggcaga tgcaagcttt tggacaccat ttattcggaa taaggaggct
    27001 gggacagaag cgtttaactc caagaaataa gaaggagttg cttctgcttc cgccgtttat
    27061 aggtgtttag agcgtgagac aaaagtgttc tttacttttg tcctacgctc cttatttttt
    27121 tattttgttt ggatcaattc ttttaattcc tctattgaaa tagattcatc aaagatttct
    27181 ttacccaaga tgacagtcgg aacaaatcga atatgtgctt gctcagcttc gttgatgaca
    27241 gcttggctca gctgttcgtc tttttgttct gtcagcttta gtttatctac tgcaaaatcc
    27301 gctacttctt ggagagaaag atgtttccac tcgtcttgag tatcgaatat ttgtttgatc
    27361 tctttgatcg ctttttgacc atcgctgatt gtgagatacc ggtgcatcac gtttcctcgc
    27421 tgcaagcttt ctttttcttt atcgaacaat ttgatcactc gctgcaattg cccgctttgg
    27481 actgcttctt ctaatagctc ataagattct tcaaaccatt gtcggcaata cggacaagat
    27541 aggttgatga attcgaccat ttttttagga gcattcgatg aaccataaag gattcctttt
    27601 tgtgtatttg ttttcgttgc atcaatcact gatatatcca tgttaaaaca cctctttcgc
    27661 ttatagttta tacatgtctg acaaaaaaag gcaacgattt agactgtttg tctaaaaatt
    27721 agatgaccaa ttcaaataga catatgtgaa atatttttat aaataaaaga aaaaatggaa
    27781 tgttttacgt tataatggaa cagataataa gagagaatat gaacaaggat gatgaatcat
    27841 gcgaaaaatg aaaacaatgg atggcaatac ggcagccgca tatatttctt atgcatttac
    27901 cgagattgct gcaatatatc caataacacc cagttcgacc atggctgaat tagtagatca
    27961 atggtcagca gcaggaaaag aaaatgtatt cggtcaacca gtaaaagtag tagaaatgca
    28021 atccgaagca ggagcagctg gtgtggtcca tggatcattg aaaactggtg cactgacaac
    28081 tacatatacc gcttctcaag ggctgctttt gatgataccg aatatgtaca agattgctgg
    28141 ggaactactg ccaagtgttt tccatgttgc tagtcgggca gtgacgacaa atgcgttgaa
    28201 tattttcgga gatcacggag acgtgatggc tgcaagacaa acaggatttg ctatgttagc
    28261 tgaaagcagt gtacaagaag tgatggattt agcacctgtt gcccatcttg cttctattga
    28321 aggagaagta ccttttttga atttctttga tggattccgt acgagtcacg agattcaaaa
    28381 aatcgaagtt ttggaatatg aagaattagc tcctctgatc aatcaggaga aattagcttc
    28441 atttcgtaaa cgagggatga atcccaatca tccgactgtt agtggtacga accagaatcc
    28501 agacatccat tttcaacaac gggaaacgat caacccctat tatgataaat tacctgaaat
    28561 cgttcaaaaa tatatgaaag agatcaaccg attacgagga acttcttatg acttggtgac
    28621 ttattacgga gcagacgatg cggaagaagt cattgtttct atgggatcag ttgcacagac
    28681 gattgaacaa acgattgatt acttgaatgc aaacggacgg aaaacaggtt ttttgaatat
    28741 ccatctttat cgtccttttc cagtggaaac attcgtgcaa aagttgcctc atacagtaaa
    28801 aacaattgct gttcttgatc gaagtaaaga accaggggca ggtggagaac ctctcttatt
    28861 agacgttcaa agtgcgttat atgattctga acttcgcccg gctgttattg gtggtcgcta
    28921 cggattgggg tctaaagatg tgactcccga ccaaatctca gcagtttttg atgaattgaa
    28981 aaaagaccca agcatacgca aaaaacggtt taccataggg atcgtggatg atgtgactta
    29041 tcaatctttg gaaccaaagg aaagtctgga tttgacagaa ccgcaaacat tccaggccaa
    29101 gttttgggga tttggatctg acggaacagt tggagcaaat aaatcagcta tcaagattat
    29161 tggagatcac accgataaat atgcgcaagg ctatttttat tatgattcga aaaaatctgg
    29221 cggtctgact gtctctcatc ttcgttttgg agatacgccg atacgttcag cgtatcttgt
    29281 cgagcatgcg gaccttgttg cctgtcacac accagcttac ctgcatagct atgacttggt
    29341 caaaggattg aagccaggtg gcattttttt attgaatact ctttggagcg acgaacaatt
    29401 agaaacgcat ctgcccttaa aattaaaacg ttacttggct gaaaataaca ttagattcta
    29461 tacgatcaat gccatgagat tggcccaaga agtcggtttg ggacgccgga tcaatacggc
    29521 aatggaaacg gcttttttta aattagctga catcattcct tttgatgagg tgcttccgct
    29581 gctaaaagaa gaagccctga aaagctatgg acataaatca atggctatcg tagaaaaaaa
    29641 catacaagca atcgatcgga cgaccgaact gcttcatcaa gtcgcgatcc cagaccattg
    29701 gagaacgttg gagatgcctg aaaaaatcaa taaaaggaac actggttata tacaagaaat
    29761 acttgaacca gtgaatgcac aggaaggaaa tcaactctct gttggtacac ttgtagcaaa
    29821 tggaatgaca actggcgaaa tgcctttagg aacggcttct atggaaaagc ggggcgtcgc
    29881 tttagaagta cctgagtgga ttagtgatcg ctgtacgatg tgtaatgaat gtgcctttgt
    29941 ttgtcctcat gctgccatcc gtccattttt agcagatgaa gaggaaatgg aagaagcgcc
    30001 agaaggattt atcgtgagag aaatgcgagg cgcagacggc gtaaaatacc gtatccaagt
    30061 ttcagtggaa gactgtacag gttgtggtct gtgtgtagag gcttgtcccg caaaagaaaa
    30121 agctttagtg atgaagcctt atgaagaaca gaaagaacaa gctgtgaatt gggcttttgc
    30181 aatgacatta agacaaaaag caaatccagt caaaggaaag aatactgtct tgtctacaca
    30241 attcaatcaa ccgcttcttg aattctcggg cgcttgctca ggctgtggcg aaacacctta
    30301 tgtaaaatta ttgacacaaa tgttcggtga tcgaatgatg atcgcgaatg cgacaggctg
    30361 ttcttctatt tggggcggtg cttctcctgc ttctccttat acgaccaatg aatgtggtca
    30421 agggcctgca tggtcaaatt ctttattgga agataatgct gaatttggtt atggtatgct
    30481 tctagcggca cagactcgtc gcgaggcttt ggcaagttca atcaaagaag tattgccaaa
    30541 agtatcagaa gaactacgag aattgatgga agactggttg gtacacatgt ttgagagcga
    30601 aggaacgcag caaagagcag caaaactgca agctgcgatc gaaagcgaac gatcaattgt
    30661 ccctgaactg gaggatatat acaagagaaa ggatctattc gtaaaaaaca gccaatggat
    30721 gattggtgga gacggatggg cttacgacat tggttttggc gggatcgatc atgtacttgc
    30781 cagcggagca gacgtgaata ttcttgtttt ggataacgaa gtctattcca atactggcgg
    30841 tcaaacctca aaagcaacac cagcttcggc aatcgctaaa ttttcggcta gtggaaagta
    30901 tgcttcaaag aaagatctag gaatgatggc gatgacatat ggaaatgttt atgtggcgca
    30961 aattgcttca ggtgccaatc aaatgcagac gataaaggct tttgaagaag cagaaaagta
    31021 tcctggacct tctttaatca ttgcctatac gccttgtatc acacatggtt tgcttggtgg
    31081 aatgaaagaa tcgcttaaag aagcaaaaga agctgtttct tctggctatt ggtcattata
    31141 tcgatataac cctgcaatgg tcgaaaaaaa caagaaccca atgactttgg actacaaaaa
    31201 acctgatttc tctgctatgc ctgatttcat gaggaaacaa gttcgctttt cctcgctaga
    31261 gaacgcaaat ccaaatcttg caaaagcttt atttgaaaag actatccaag acgctaaaac
    31321 acgtttttac aactatgctg cactgacggg agaattagaa aaaatcaaga aacgacttga
    31381 accagaggaa tctatcgatg tacccgttaa gactagaaaa gaaaaaacga cggaccttga
    31441 agcagaagaa agaagagctg ccagagcaga acgacgtaaa aaaatggaac aagctacaaa
    31501 agaaaaaaag gaaaaataac agaaaaaata taatgaataa ttctctgaag tttaaaaata
    31561 tttattgcag agaaaaataa aacgcatact tgcaattttc ctgaaaatta tgcgtttttc
    31621 tcaagaaaaa tacgagcaga tcagcctcta aaattaaaac atatgatata ctagagagga
    31681 aatctcaaaa gagaagagag gctttttatg tcatttcaaa tcggggaact ttttaatttg
    31741 gactattggc agcaaatgtt ttccggtgac ttgttctctc tgaatttttt ggtgaacata
    31801 cttgatattc tggttgtctg gtatcttgta tacaaactga tccaactgct gcgaggcaca
    31861 aaagcgattc agcttctgaa aggtgtagct atttttattg ttatccgaat cgtagcggaa
    31921 atcattggtc tgcacacctt gtcttggctg atgaaccaag tcattacgta cggggtgata
    31981 gctgctatcg tgatttttca gccggaaatc cggagaggac tggaacatct tggacgcagt
    32041 tcgcttttca aacaatcaaa aagcgaacag caggaagatg agaagatgat cttgtctttt
    32101 gacaaagcca tccaatatat gtccaagcga aaaatcggtg cgctaattac tatcgaacgg
    32161 catactggat tagatgagta tattgagaca ggaattgcat tagatgcaga tatcactgga
    32221 gagctgctga tcaatatctt tattcctaat acaccgctgc atgacggtgc agttattgta
    32281 aaagaaggaa agatcgcagt agcaagcgcc tatcttcctc tatcagaaag tatgttgatt
    32341 ccaaaagaat tcgggactcg acaccgggca gctgtcggta taagcgaagt cagcgacgcg
    32401 attaccatcg ttgtctctga agaaactggg gatgtcagca ttacattaga taatgaactg
    32461 atggctggct tatcccagca agaatatttg gcaattttgc gtagagaact gattgctgaa
    32521 gagaaaacaa aaaacaagaa aaacgtgatt caatctttcc tagacggtat gactaaggga
    32581 ggggatcgca aatgatctca aaagagaaac ggacaagcat tctttacagt tttattgcat
    32641 tgctgttcag tattgcgctg tacttcaatg caaatggcca gtccgttcca aatacgcttt
    32701 ctggaaatga agcgtataat caaacagtta ctggggtgcc aatcaagatt tcttatgatt
    32761 caaagcgcta ctatatccat ggatatgaaa atgcagtgac tgtcaaactt tccagtgcaa
    32821 accgagttca gttgaatgca gaggcaaacg aagatacacg gatgttccgc gtcacagcag
    32881 atatcactaa gctgggcgaa ggaacacatg aggttccgct aaaaattcaa aatttgagca
    32941 gtgctgtcac agcaaagatc gaaccatcga ctattacagt aacggttgag aaaaaggtaa
    33001 taaaagattt cgatgtggaa acgttgattc cttcaacgtt cacaccatct ggatatgaac
    33061 ttgatgatac ttccgttagt cctaaaaaag tctctatcac tacaggtgac aaaacattgg
    33121 cagaaatcaa acgagtagta gctgatgtgg acgcttcgat ggtcacagat gatgggatta
    33181 atagtgaggt cccaatacag gcgctgaatg cagcagggga agtattaagt atcgtctctg
    33241 atccagtgca agtaacagtg agagctgatt cgatcaagcc aagcaagtcg gtacgacttt
    33301 atgggataca gcaaggaaca ccagctgccg gggtagaaag ctatgatttc agtttttcaa
    33361 aactggaagc agaagtaagc ggctctagcg atttgcttgc atcgatcggt gacagtattg
    33421 ctgtaccgat caatgtctca ggtatcaatc atcgaacaac tcgtaaaatt gaaataccag
    33481 tagaagaggg gttaagtatc aagcccgaat cagttagtgt ggagattaca cccgtgttgc
    33541 aaacgtcaag cagtagctct gcttcgacga gcagtcggac acaagaaaat acaagtacga
    33601 gtagaagtat ttcatctgct gattcaggaa cggaaccatc gacaacaacc atttcttcca
    33661 gttcggaatc tgggacaagc agctcgacca caagtacaga agaaacatca gaaacaagcg
    33721 aatcaacaca aatgagcaga acaaattcct cagaaaatta agttaaagga gtaattttac
    33781 aactatgggt aaatattttg gaacagacgg cgtacgcggt gaagccaaca aggaacttac
    33841 gccagaactt gcatttaagc tgggacggtg cggaggttat gtcctaagcc agcatgaaga
    33901 agaaggaaaa cgcccacgcg tgttagtagg gcgggataca agaatctcag gtcaaatgtt
    33961 agaagaagcg ctgatttcag gacttttatc ggtgggtatc gaagtgttcc agctaggtgt
    34021 gatctctaca ccaggtgttg cttatctgac acgtttgcaa aaagcaagcg caggggtcat
    34081 gatttctgct tctcataatc cagcacaaga taatggaatc aaatttttcg gtgcagatgg
    34141 ttttaaacta gtagatgacc aagagctaga aattgaagca ttattagatg ctgaagtgga
    34201 tgaattgcct cgtccatcag ctgaaggatt gggaactgta gaagaatttc cagaaggctt
    34261 attgaaatat tctcaattcc ttgtacaaac gatcaacggt gacctttccg gtttgaccat
    34321 ttgtgtcgat gcagcaaacg gagcaacttc aacaagtgtt aatcgtttgt ttgccgatct
    34381 tgaaacagat ttttatacca tgggcacaaa tccagacggg ttaaatatca atgatggcgt
    34441 aggctctacc catccagaac gtttagctga gatggtcgtt gaaaaaggag cagatgctgg
    34501 actagctttc gatggcgatg gcgatcgtat tattgcggtg gatgaacttg gaaacattgt
    34561 cgacggtgat aaaatcatgt ttatctgtgc taaatacttg gcagaaaaaa atcgattgaa
    34621 aaaagatacg atcgttacga ctgtcatgag taacttagga ttccacaaag cggtggaagg
    34681 aatcggtttg aaagatgtcg ttacacaagt tggtgatcgt tacgttgtag aagaaatgcg
    34741 caaaaacgat tataactttg gcggcgagca gtctgggcac atgattttct tagattttaa
    34801 cacaactgga gatgggatgc tttcaggaat tcagttactg aatatcatga aacagacagg
    34861 taagaaatta tcagagcttg cagcagaggt aacaatctat ccgcaaaaat tagtgaatat
    34921 ccgagtaagt gataaaaacg gtgcgatgga tgttccggca atcaaaaaag tgattgaaga
    34981 ggcagaagct gaaatgggat cagaaggaag aatcctagtt cgtccttccg gtacagaacc
    35041 attacttcga gtgatggcag aagcgcctac tgatgagaaa gttaattact atgtcgataa
    35101 aatcgcggca gtagtcaaag aagaaatcgg actggattaa gagatatttt cataaa
//
LOCUS       DAYYGO010000014        34959 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000014 DAYYGO010000000
VERSION     DAYYGO010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.014"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            153..1859
                     /gene="efbA"
                     /locus_tag="ACHA5T_000608"
     CDS             153..1859
                     /gene="efbA"
                     /locus_tag="ACHA5T_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFM8886210.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2017..2919)
                     /locus_tag="ACHA5T_000609"
     CDS             complement(2017..2919)
                     /locus_tag="ACHA5T_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8886211.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2916..3608)
                     /locus_tag="ACHA5T_000610"
     CDS             complement(2916..3608)
                     /locus_tag="ACHA5T_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFM8886212.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3651..4292)
                     /locus_tag="ACHA5T_000611"
     CDS             complement(3651..4292)
                     /locus_tag="ACHA5T_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFM8886213.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4330..4644)
                     /locus_tag="ACHA5T_000612"
     CDS             complement(4330..4644)
                     /locus_tag="ACHA5T_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886214.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4632..5261)
                     /gene="pyrE"
                     /locus_tag="ACHA5T_000613"
     CDS             complement(4632..5261)
                     /gene="pyrE"
                     /locus_tag="ACHA5T_000613"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFM8886215.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(5268..5984)
                     /gene="pyrF"
                     /locus_tag="ACHA5T_000614"
     CDS             complement(5268..5984)
                     /gene="pyrF"
                     /locus_tag="ACHA5T_000614"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFM8886216.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(5981..6913)
                     /locus_tag="ACHA5T_000615"
     CDS             complement(5981..6913)
                     /locus_tag="ACHA5T_000615"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFM8886217.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6910..7686)
                     /locus_tag="ACHA5T_000616"
     CDS             complement(6910..7686)
                     /locus_tag="ACHA5T_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFM8886218.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7740..10925)
                     /gene="carB"
                     /locus_tag="ACHA5T_000617"
     CDS             complement(7740..10925)
                     /gene="carB"
                     /locus_tag="ACHA5T_000617"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFM8886219.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10930..12006)
                     /locus_tag="ACHA5T_000618"
     CDS             complement(10930..12006)
                     /locus_tag="ACHA5T_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFM8886220.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12011..13291)
                     /locus_tag="ACHA5T_000619"
     CDS             complement(12011..13291)
                     /locus_tag="ACHA5T_000619"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFM8886221.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13310..14236)
                     /locus_tag="ACHA5T_000620"
     CDS             complement(13310..14236)
                     /locus_tag="ACHA5T_000620"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFM8886222.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14249..15532)
                     /locus_tag="ACHA5T_000621"
     CDS             complement(14249..15532)
                     /locus_tag="ACHA5T_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFM8886223.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15546..16085)
                     /gene="pyrR"
                     /locus_tag="ACHA5T_000622"
     CDS             complement(15546..16085)
                     /gene="pyrR"
                     /locus_tag="ACHA5T_000622"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFM8886224.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16320..17231)
                     /locus_tag="ACHA5T_000623"
     CDS             complement(16320..17231)
                     /locus_tag="ACHA5T_000623"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM8886225.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17228..17692)
                     /gene="lspA"
                     /locus_tag="ACHA5T_000624"
     CDS             complement(17228..17692)
                     /gene="lspA"
                     /locus_tag="ACHA5T_000624"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFM8886226.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17697..18395)
                     /locus_tag="ACHA5T_000625"
     CDS             complement(17697..18395)
                     /locus_tag="ACHA5T_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFM8886227.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18382..18768)
                     /locus_tag="ACHA5T_000626"
     CDS             complement(18382..18768)
                     /locus_tag="ACHA5T_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886228.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18915..20585)
                     /locus_tag="ACHA5T_000627"
     CDS             complement(18915..20585)
                     /locus_tag="ACHA5T_000627"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFM8886229.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20768..21001
                     /locus_tag="ACHA5T_000628"
     CDS             20768..21001
                     /locus_tag="ACHA5T_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8886230.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21100..21531)
                     /locus_tag="ACHA5T_000629"
     CDS             complement(21100..21531)
                     /locus_tag="ACHA5T_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFM8886231.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21610..22023
                     /locus_tag="ACHA5T_000630"
     CDS             21610..22023
                     /locus_tag="ACHA5T_000630"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFM8886232.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22061..23209)
                     /locus_tag="ACHA5T_000631"
     CDS             complement(22061..23209)
                     /locus_tag="ACHA5T_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM8886233.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(23331..23927)
                     /locus_tag="ACHA5T_000632"
     CDS             complement(23331..23927)
                     /locus_tag="ACHA5T_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8886234.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(24127..26769)
                     /gene="alaS"
                     /locus_tag="ACHA5T_000633"
     CDS             complement(24127..26769)
                     /gene="alaS"
                     /locus_tag="ACHA5T_000633"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFM8886235.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27157..27588
                     /locus_tag="ACHA5T_000634"
     CDS             27157..27588
                     /locus_tag="ACHA5T_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8886236.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(27633..28982)
                     /locus_tag="ACHA5T_000635"
     CDS             complement(27633..28982)
                     /locus_tag="ACHA5T_000635"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM8886237.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(29052..30215)
                     /locus_tag="ACHA5T_000636"
     CDS             complement(29052..30215)
                     /locus_tag="ACHA5T_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM8886238.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            30344..30667
                     /locus_tag="ACHA5T_000637"
     CDS             30344..30667
                     /locus_tag="ACHA5T_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886239.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(30850..32199)
                     /locus_tag="ACHA5T_000638"
     CDS             complement(30850..32199)
                     /locus_tag="ACHA5T_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8886240.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(32268..33221)
                     /locus_tag="ACHA5T_000639"
     CDS             complement(32268..33221)
                     /locus_tag="ACHA5T_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFM8886241.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(33323..34513)
                     /locus_tag="ACHA5T_000640"
     CDS             complement(33323..34513)
                     /locus_tag="ACHA5T_000640"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFM8886242.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            complement(34713..>34959)
                     /locus_tag="ACHA5T_000641"
     CDS             complement(34713..>34959)
                     /locus_tag="ACHA5T_000641"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886243.1"
                     /translation="IRKNRTVTRLLSQSFFRIQQVKLLRNIQRVKLKIADKRRWKFYV
                     FLLVYAINKLNIQNTIDFNERASINGCVVFICPGRVK"
BASE COUNT         9951 a         7640 c         6120 g        11248 t
ORIGIN      
        1 taaaaatttg gatttcaacg atagaaacac tgttttttat gtaattagtc caagattttt
       61 ctagactttt gctttactat tcggccaaat tggtttttct tctttttttc gttataatga
      121 atatacttat gaaaagaaat gaggaatgaa ccatgtcctt tgatggtgta tttacccacg
      181 cgatggtcaa cgaattgcgt gaaacgttac tatctggccg gatctcgaaa atccatcagc
      241 cttatgaaaa tgaaatcgtg ctggtgattc gttcacgcgg caaaaaccat cgattattat
      301 tatctgcgca cccaagttat gctcgtgtac agattaccca aatcgattat cagaatcctg
      361 ataatccgcc taacttcgtg atgatgcttc gaaaatattt ggatggcgct attttagaag
      421 acatcgaaca aatcgaaaat gatcgagtga tccatttcca ctttgctaaa cgaaacgagt
      481 tgggtgatct acaaaatatc atattgatcg ttgaattaat gggacgtcat agtacgatcg
      541 tcttagttaa tcgagaaact ggaaaaatac tggatgcgat caaacacatt ggaagctctc
      601 aaaacactta tcgttctttg cttcctggtg tagaatatgt tgctccgcca aaacaagagg
      661 tcttgaaccc atttagctcc gagaaagaaa aaatctttca gcggctttcc caaactgaac
      721 tagatccaaa agcaatccaa cgtcagtttc agggaatcgg tttcgatatc gctcaagagc
      781 tgaccaaaag attactggaa cgtcctaatg aaaagatggt tgtatgggac gaatttttct
      841 cagccatcag ccatcagcct attcctactt tttacgaaac ggaaaataaa gattttttca
      901 caccgattgc ttatcaggta ttgtccgaac aggcatctgc tgtaacagct tatccaacac
      961 tcagtcaatt attagatagc tattatcacg aaaaagcaga gaaagatcgg gcaaaacaac
     1021 aaggcggaga actgattcga aaaatcgaaa atgaactaaa acgaaataaa aataaattga
     1081 aaaaacgtga acaaacactc aaagaatccg aaaacgctga gaattaccgt agaaatggcg
     1141 aactacttac tacttttctg acacaagtac cacgcggcgc aaaagaagtt gttttaccta
     1201 attattatga agaagatcgg ccaatcaaaa tagcactaga tccggcattg acaccaaatc
     1261 aaaatgcaca gaaatatttc catcgatacc aaaagctgaa gaacgctgtt aagctgattg
     1321 gcgaacaaat ccaagaagcc aaagatgaaa tccaatatct tgaatctgtc ttatctcaac
     1381 tggaaattgc cggcccaatg gatatcgaag caattaaaga agaattgact tcagaaggat
     1441 acctgaaaaa gaagaactta aaaaaacaaa aacgtaagaa accatctcag cctgatcaat
     1501 acttttcttc tgacggaacg ctcattcttg ttggtaaaaa caatcttcaa aatgaccagc
     1561 tgacaatgaa gacagctaaa aaaaccgact attggcttca tgcaaaaaat atacccggtt
     1621 cgcacgtcat cattaaaagt gacaagccct cagatgaaac aattacagaa gctgccgagt
     1681 tagctgccta tttttcaaaa taccgatact ctgcccaagt gccggttgat cttgtacagg
     1741 taaagcatat ccgaaaacca aatggcgcca agcctggtta tgttatttat gaaaaccaaa
     1801 agacgattat cgtgacacca gaagaagaaa aaattacagc tatgaaacaa aacaggtaat
     1861 caatgagtgt ttctagaatc atatttgcgt actttcccac aacagcaatt agttataaat
     1921 tgtttgctac aaaaagagga gttgtgacaa aattctgtca caacccctct tttttgatca
     1981 acggtgttca ataaacagcg ttctcatctg cttttcttag ctctgctggt ccagtaaccg
     2041 agtaagctct ttgataaatc tttgagcgat ttcactgagc ggacgtttcg ccgaataaat
     2101 gatataaaat tcatttagtt cgccttctgc caaagggatt aaccgcaagt gctcgcgaat
     2161 ccctccaatc acaataccag atcctgaagc gtaagcatca gtttcaagaa cgatattcat
     2221 caaagtacct cgatcactcg tataaatcac tgtttcttga tcagggatct cgataaggtc
     2281 ttcataaaaa tacgggaaat tatttccatc ctgtgtaaaa cgaacttgag gataagataa
     2341 caattcttct ttggtgatct ctgcgtgttc agctaatgga tgtccttgcc ttaaaaaaat
     2401 atgcgttttg aattttccaa tgctgcggcc acttagctct ccttgttgta aatatcttct
     2461 gataccagct tgattatgct cattcatata aataatgccg atctcactgt gctgctcttt
     2521 gacttcttcg atcactctcg ctgttgtcgt ctccatgatc cgaaattgtt tgatctcgtc
     2581 tttgaattcc tcgagtagct gtgcggtcaa aggacctaag aaatcataat gctgggcagc
     2641 gatcgaaaaa ttgtctctct tttcactcgt tagatagtga gtttctaatg tatcaagctg
     2701 gaccaatatt ttctcggcac gatttaagaa gtcttgccct tcttctgtta aatatgcccc
     2761 tcgattcgtt cgagtaaaca gcgtgatccc taactcggtt tctaattctt tgataccagc
     2821 tgaaagactt ggctgggaga caaacatttt ctttgccgct tctctgaaac taccattgtt
     2881 tgctacagcg accacgtatt tcatctgctg aatattcatt gctcaaactc ctcgaaataa
     2941 taaatttctc ttccattcaa gggctggatt ggtcggttgt tcggtcgagc atactgacca
     3001 tcgaagggga gaagaaactc ttggttcagt tttcctacat gatccattac aaaccattgg
     3061 attggcccgc tggtaggcgg agttgtcaat gagccaatat aatggaaatg gcttttttgc
     3121 tccggaaaga acacatccgg atttacccaa tgctcatgtt tttctaagtt ccaactttcc
     3181 ccattttctt gattatacag ccaaccttcc tgtaccatta gctggtacat gacacccaca
     3241 actagattct cttttttgct gttcatatga acaaaatgaa attcaatatc ttcctgtttt
     3301 ccgttgatga tatgttcaga aggcaagtgg aaatgaatat ctgtcaaata atagcgttct
     3361 ttcttgaact ctacataact gagttggtca aaaggtacta aatgaatcgt gtttttgaat
     3421 tccttgtcag taaaccgttg cttttgataa tgaaaggaaa gcgtgtctcc ttctattttg
     3481 gtttcttccc ccttggtcaa atgaataggc gactgcaatg gaaattccgc acctctcgca
     3541 aaccaatcac ataaactcgc ccaattttcc ggacctttgt ctccagaata gccccattcg
     3601 acatccatat tctttttcaa ctatacttcc ccctgcatct catatcattt ttatttttct
     3661 tctttcttta caatcgttga gacggtttct aaaaacgcct cttttccata aaattcctgg
     3721 ttcaatagtt ccggaaatat ctctttaaag aaatactgaa cagaaggccg atgttcaaag
     3781 cttttaatcg tagtcactgc ctcaatgaac atctccttat aaactttttc cgggttattt
     3841 ttgatgattt catcaaaaga ttcataaaga aggtcaacct tatctgaaat ggctaatagt
     3901 tctccttcta cggtttcatc ttttccttcg aacaaccgac ggcgataaac ttcttggaac
     3961 tcagctggaa tctcttggtt gatgaattcg tccgtcatct tttcttcaac tgtttgcagc
     4021 atccctctta actctttgct tgcgtatttc accggcgttt tgatatcacc aataaaacgc
     4081 tctgtgtagt catgattcaa tgatttttca tagagcagtt tccaatcgat cggttgtcca
     4141 ttcgcttctt caatgtctcc tagaacttgc gcaatagatg ctacacgata cgaatgtgct
     4201 gcaactgtat gctctgtata tttaaaaaat ccaggtgccc ttgtgatcgt ctctaaattg
     4261 ttcaatccta aaataaattc attcaacccc ataaaagtta cctccctttg agatttcttt
     4321 ttccgaatat catatcatta aataactgat attcgcaaca tttccgctct ttaccatttt
     4381 tgaaacagct cccttaccaa aaaatagaga aaatctatat ataatagaaa aaagagaaga
     4441 gattattctc ttctttctgc taaccagtct tagaaaaatg aattttaaaa aaatcaaaaa
     4501 ctgatcacga tagaagtttc ttttcatacc tgatgaaaag atgcttctcg caatcagttt
     4561 ttggattttt gctattccaa ttgatgattc tggagacaca aatcggattt ctctatcttc
     4621 atcaagcgcg cttaaatctg ccaattttca ggatcttttt tccattcttt tagcagagtt
     4681 aattcttgct catcgatata acgatcttct aaagctgctt caatcaatac agaataattg
     4741 gtcaacgtca taagagggat ttcggctttt tcgaaatttg cttttccttt tggcaactca
     4801 tacgtaaaga tagccgcaac accaaggata tccgcacctt ctctttttgc tgcttcagct
     4861 gcttccaaga cacttccgcc tgtagaaatc aaatcctcga tcacaaccat cttctgacct
     4921 tcaaatatac gtccttcgat ttggttacct ttcccatgat ctttcgcctt acttcgaata
     4981 tagaccattg gcaaatccag tatttccgct acccatgcgg catgagggat tcctgctgtt
     5041 gcagttccag caatcacttg tacttcaggg taagcttctt tgattttgct tgctaatcct
     5101 tttgcgatct cttttcggac ttttggatag ctcatcgtga ttcggttatc acagtaaatt
     5161 gggcttttga ttccacttgc ccatgtaaaa ggattacttg gatttaaaaa tactgcttca
     5221 atttctaata agtcttttgc gatactttgt tcaacattca ttttaggtta gctccattct
     5281 gtttgaatcg tttgatatgc tttataagga ttagttgact gagtgattgg tcgacctata
     5341 acgataaaag ttgagccgat cttttttgcc tgagaaggtg tgactactct tgtctgatct
     5401 cctgcgactg ttccttctgg tcttataccc ggagttaagc aaacaaattc gtctcctgcc
     5461 atttctttga tcgcttctac ttcccaagca gaagaaacca caccatctaa tcctgctttt
     5521 ttggcacagc tagcataatg gatcacactt ttttctaaag aaacttcgat tagttgatcg
     5581 gcatgcattt cttcttcact tgtagaagta agctgagtga tagctagaag tagcggacgt
     5641 tttccacctt ctggagtccc ctcttctaat cctcgcattg ccgcctccat catacgaata
     5701 ccgccagcgg catgaacaca ggtgagatca acaccaagct ttgccaatcc ccgcattgct
     5761 ttttcgaccg tattcggaat atcgtgaagt ttcagatcta aaaatacatt atgaccagcc
     5821 tcttttagat aacgtacgat ttcaggtcct tcttggtaga acaattccat tccgattttc
     5881 acaaacaatt tttcttcttt cgggaaaaat cgaagaaagt cttctacctc ttgcctagtt
     5941 gaaaaatcta atgcaataat tggtcgttgg tctagttgat tcatcgttca cgttcctctc
     6001 tcacttcttt gattaatctt tccagcgatt cgatgcctag ttcgctcatt cgcacaggga
     6061 gctctttaat tagtttagga cagatatacg gatcggtgaa attcgcggtc cctatagcta
     6121 cagcacttgc tccagccata aacatctcaa gaacatcatc cactgtctgc acgccgccca
     6181 tcccaatgat tggaaggctg gatatacttg ctacttggtg aatcaaacga atagcgacag
     6241 gcttgattgc tggtccggag agtccacctg tttgattggc taaaatcggt tttcttgtct
     6301 taaggtcaat ccgcatacca agaagagtat tgatcatcgt aaacccatct gcccctccag
     6361 cttcgattgc ctgagcgatt ggaacgatgt ctgttacatt tggggacaat ttgacataga
     6421 caggaacaga agaaactttc tttactgctt gtgttaattg aaaagcaaca tcagaatctg
     6481 ttccaaaagc aattccgcca tgtttgacat ttgggcaaga aatgttcagc tcgattgctt
     6541 tcacatttgg tgcttcgcca attttgctac aaacttctac atagtcttct tcacaagcac
     6601 cagcaacatt tgcgatgatg ggtaactcat tgtactcctc taattctgga agaagtttat
     6661 gcataacaac gtctagacca ggattttgca aaccaattgc attcaacatg ccagatggtg
     6721 tttccgccac tcttggcgtg ggattgccaa agcgggcatt tggagtcgtt gccttgacca
     6781 tgatcgatcc aagctgattc aaatcatagt attttccgta ttctttgcca aaaccgaaac
     6841 agccgcttgc cggcatgata ggatttttta attctaggcc aggaagtttg accgctaaag
     6901 gattctcatt catataatta cctctcctat tttaaaaatc ggtccttcgt cacaaacttt
     6961 cacacttttt gttcctgttt catcttccgg cgtatgacaa acgcaagcat agcaagcccc
     7021 tatcccacat gccatgcggg attctaatga taactggaca ttttgatgcg aagaaaacag
     7081 ctgttccact gtcttcaata acccgtttga accacatgca aaaacagctg taggttccgt
     7141 cttcgttcct aacaataaat taccgacatt tccgtgcata ccgtatgttc catcgtcagt
     7201 agcgattctt gtttctccta gacgattaaa ttctttttca taataaacga cttcatgtgt
     7261 ggcaaaacct aaaaaatgcg ttggcttaat tcctttttcg actaactgtt ttgataactc
     7321 atatagagga gggacaccga ttcctccacc gattataaat acttcgtctc ctgtatgaag
     7381 attggacaaa tcaaaaccat tgcctaaagg acccatgaca tctaaatggt ctccggcaga
     7441 aagagtagag aagactctag tcccctcacc ttccacccga taaatgatac ggcaagtatt
     7501 tgtttcgtgg tcgatatgat tcaagctaat cggtcggcgt aatagcaagt ctgcacgcgg
     7561 cacgagaata tgcaagaact gtcctggttg ctgcatttct tttactagat cacctttcaa
     7621 tgtcatttca aaaatctttg gtgccagctg tttttgctga acgatttcca tcatttcttg
     7681 tttcatctgt cccctcctgc aacggttttt cgtctttttg tcttaacatg tgctcgccgt
     7741 taaatagggt tagttgaaaa agcacgggat tccatcactc gaacgatcgc atctgctgtg
     7801 tctaaagaag taaacaatgg aataccatgt tcaactgctt ctcttcggat gatgaaaccg
     7861 tctttagaag catttccccg atctttatca atcgtattga caaccacttg tgttttacca
     7921 tctcttatta agtcgactac atttttttct gctgcttcct cagaaatttt cgctactgtt
     7981 tcaacgacca gaccattttt ttcgaaataa gcagctgtat gtttcgttcc tatcagacta
     8041 tagccaattt ctgtaaaacg tttagctaat tctagtgctt cttcttttgt ttcgtccgca
     8101 atcgtaaata atacggctcc atattcaggc aagtgtaatc cagaagcttc gaatgccttg
     8161 tacaaagctt tgtctaattg tctgtcagat cccattactt ctccagtcga tttcatttca
     8221 ggccctaagt aagtatcaac tttctgcaat ttcgtaaaag aaaagacagg tgctttaaca
     8281 tgaacttctt ttgtttctgg ataaagtccg ttttcataac caagtgtctt tagtttctct
     8341 cccaaaatag ctttcgtcgc aatttgtgcc attggaattc ctgtcacctt acttaagaat
     8401 ggaacagtcc gacttgctcg aggattgact tcgatcacat aaacctcatt ttcatggatg
     8461 acaaactgga tattcatcat cccgacacaa ttcaaaccta atgctaagcg gatcgtgtaa
     8521 tcttcgatcg ttttcttgat cgattcagat aatgtttgag gtggatacac agccattgaa
     8581 tcccctgaat ggacaccagc acgctcaata tgctccatga tccctgggat gagtactgtt
     8641 tcaccgtcac aaatcgcatc cacttcgcat tcaccaccaa gcaagtaacg gtccactagt
     8701 actggatgtt ctggagatgc tttcacagca tgttccatgt aatcttctaa atctcgctgg
     8761 ttttcaacga tttccatcgc acgtccgcct aatacatagc ttggacgaac aagaactgga
     8821 taaccaattc tcgaagcgat aaccacggct tcttctttac ttgtagcagt atcccctaaa
     8881 ggttgaggaa tatccaactc tttcagcgct tgctcgaata aatcacgatt ttccgcacga
     8941 tccaagtctt cgatcgttgt ccctaagatt tttacaccag cagcagtcaa aggttcggct
     9001 aagttgattg ctgtctgacc accaaattga acgattactc ctatcggctg ttctagctcg
     9061 atcacattca tcacatcttc gaaggtcaat ggctcaaaat ataatttatc cgaaatagaa
     9121 aagtcagtcg aaactgtttc aggattgctg ttcatgatga ttgcttcgta tcctgcctgc
     9181 tggattgctt tgacagaatg cacagtcgca tagtcaaatt caaccccttg tccgatacgg
     9241 ataggaccag atcccagtac tagaattgag ggcttttctg atcgtgaact ttcattttca
     9301 tattcatagg aactataaaa ataaggtgtt tttgattcga attccgctgc acaagtgtca
     9361 accatcttat atacaggaat caaatcattt tcttttctca tctggcgaac ggcttcttcg
     9421 tttatttccc acagctccgc cacttttttg tctgtaaaac cgttttgttt cgctgttttc
     9481 aatatcttgt catccaacgg ctgctgtttc aactgctgtt cgatttctac gatatgcaac
     9541 agtttatcta aaaagaataa atcgattttt gtcaaatccg ctaattcttc tatggtgtac
     9601 cctcttctaa tagcttcagc tacataaaac aggcgatcat cttgtgctcg aaccactttt
     9661 tggattaggt catcgtccgt gatgtctgcc aactcaggaa gttcgatatg gtgtgtaccg
     9721 atttctaaag aacgcacagc tttcaataaa gactcttcga tattccgacc gatagccatc
     9781 acttcaccag ttgctttcat ctgcgtgcct aaaagccgtt ctccgttttc aaacttatca
     9841 aacggccagc gaggaatttt agctacaaca taatctaatg caggttcaaa ttctgcaaaa
     9901 gtcgttcctg taacaggatt tttcatttcg tctaatgtta atccaagcgc tattttagca
     9961 gctagcttag cgatcggata gccagtcgcc ttacttgcta acgcagatga acgagaaaca
    10021 cgcggattga cttcaatcac ataataatta aaactatgtg gatcaagagc gagttggaca
    10081 ttacagcctc cctcgatctt aagtgctcga ataatcgaaa gagaagcatc acgaagcatt
    10141 tgatattcat ggtcagacaa tgtttgacta ggggcaaata cgatagaatc acctgtatgg
    10201 atacccaccg ggtcaaaatt ttccatattg cagacaacga ttgcattatc agccgaatct
    10261 ctcatcactt catattcaat ttctttaaaa cctgcaatac ttcgttcaat caaacattgg
    10321 gtagcggggg aaagacttaa tccattttct gatacgatac gtaattcttc ttcattttca
    10381 cacatacctc caccagttcc gcctaaagta aaggctggac gaacgatgac aggatagccg
    10441 attttatttg caaaatctac agcttcttct actgtattga cgatttcaga ctcggggatt
    10501 ggctgattca attctttcat caactgtttg aatagatcac gatcttctgc ttgatcgatc
    10561 gcagaaagct ttgttcctag aagttctatg ttcaattctt ctaaaatacc tgatgctgcc
    10621 agctccattg ccatattgag gcctgtctgt ccgcctaaag ttggaaggag agcatctgga
    10681 cgctcttttc gaagaatcct tgatacaaat tcaagtgtga tcggttcgat atatacttga
    10741 tctgccactt ctttatccgt catgatcgtc gcaggattag aatttactaa aaccacttca
    10801 tatccttctt cttttaaggc aaggcaggct tgtgttcctg cgtagtcaaa ttctgcggct
    10861 tgaccaatga tgatcggacc agatccaatc accataattt tcttgatatc tgtacgtttc
    10921 ggcatctgtt tactgctcct ttcctgcgtc catcatttcc ataaattcat caaataaatg
    10981 tagtgcatca tgcggaccag gggcagcatc tggatgaaac tgcacactga aagcgggata
    11041 atctctgtgt ttgactcctt ctactgttcc gtcatttact tctacatgag ttacgatgag
    11101 tttttctgga tcaatcgtgg cttcatcgac tgcatagcca tgattttgag aagtaaaatc
    11161 aattcttccg gtcgctactt ctcgtaccgg gtgatttaat cctcgatgtc cgaatttcat
    11221 tttataggta tctgcaccgt tggctaaagc aaataactgg tgaccgagac aaataccaaa
    11281 aatcggtact tttccttgga tttttttgat catttcgatt gcttctggca catcttttgg
    11341 atcaccaggt ccattcgtca gcatgactcc gtctggtgat agcgctaaaa tcgtctcggc
    11401 atctgtgtta tagggaagta ctgtgaggtt acagtttctt ttactcaatt ctcgtaaaat
    11461 actatgtttc aagccgaagt caatgacaat gactttccga cctgttcctg gacttggata
    11521 aggtttgtta gttgagactt gctgcacttg attggttgct agaacggttg ctttcagctg
    11581 atcaaatgca tgttcgaagg aatctccagc atcaactata ctagctttca tcgttccgac
    11641 ttcacggatt ttgcgagtaa gggcgcgcgt atcgatacct gatattccag gaattccttt
    11701 ttgtttcaaa aatgaatcta gcgtcatttg gtttcgccag tttgaagcta cacgtgcatg
    11761 ttcttttact actactcctt tgcatgttgg agaaatcgat tcataatcat ctctatttac
    11821 cccataatta cccaccatag gataggtaaa tgtaatgatt tgaccgttaa aactttgatc
    11881 agtgatagtt tcttgataac cagtcatact tgttgtaaaa acgatttctc cgaagacact
    11941 agcatctgca ccaaaagcct ttccttcgaa aactgttcca tcttctaata taagcaaacg
    12001 ttccatactt ttattctccc ttctgccaca caagttttcc gttgacaaaa gtcatttgtg
    12061 tttcgccttt tactttccag ccgataaaag gtgtattctc cccttttgat aaaaagtctt
    12121 ctttatcgat cgtgcaagtt tgtgtaatat caaaaactgc tacatcagcg ggggcaccaa
    12181 ccgttagcgt tcctgcattc aaaccaaaaa tctcagccgg tttaactgcc aaccaattga
    12241 tcacttgttc caacgtgaat cgaccagttt ctacaaaatg agtatagatc agttgaaacg
    12301 ctgtttcgct tcctacgata ccaaatggtg atttcatgaa actttggctt ttttcttcta
    12361 atccatgggg ggcatggtct gtagcgatgc aatcgatcgt accgtcaagc aagccttcga
    12421 tcaaagcttc tctgtcctcc cgtcctctta gcggcgggtt cattttccaa aagccgaaat
    12481 cttcgggaat atcttcgtcg atcaaaataa gatgatgggg tgaaacttct gctgttacgt
    12541 ggattcctgc tttttttgcg tctctgatta cacggacact ttctttagta gaaacatggc
    12601 aaacatggta atgaacacca gttgcttcag ctagcaacag atctctagca atttgagaag
    12661 actcggttac gcttaagatc ccgggaagac caagttcttc tgccttcttg ccagcgtgca
    12721 taacgccgcc aaataataaa gattcatctt ctgtatgggc tactagggct ttattgtttt
    12781 ttgcagcttc tttcatagcc agatacatcg tcccagctgt ttggacacct acaccgtcat
    12841 ttgtgaaagc aaaagcacct tcttcaatca gtgcttcttg atcaactaat ttctcacttc
    12901 gcagattttc tgtgattggc gcatactgca ataccttcac aactgcatct tttctaatga
    12961 tgtcatatac ttgtctcagc ttttctgccg tatcaggtac aggatttaaa ttcggcatcg
    13021 cgcatacagt agtgaaaccg ccacgagcag ctgagcgagt cccagcttcg atcgtctcct
    13081 tgtatgtaaa tcctggctct ctcaaatgaa catggacgtc cactaaacca ggtgtgatca
    13141 attgtccctt tgcatcaaac acttcatcaa attcggcttc tgagaatcct gtaccgattg
    13201 ctttgattcg cccatcttct atccaaattt ctgctggtgt cgtcatattt tttcgtgtat
    13261 tgatctgacc gtttttaatg agtgttttca ttttttcatc ctcctataat tacgcttttc
    13321 catttagcac tgcttctaag attgccattc tgacgaatac tccattgctc atttgctgaa
    13381 cgatccgcga ctgtaatcct tccactaatg aatcagctaa ttctacatca cggttaactg
    13441 gtgctgggtg cataatgatt gcatgttttt gcaacttctt cgcacgctca actgttaacc
    13501 catgttcctg atggtagcct tcttttgaga aactttcttt tccatcatgg cgttcgtgtt
    13561 gcacccgaag catcatcatg acatcaactt gatctagtag ttcatccaat ggcatataat
    13621 gtccgtacac ttcgaattca tcgtcgtacc attctctagg acctgagaag aatacttgtg
    13681 cgcctagacg ttttaacatc tgcatattgg atttagctac gcgagagtga gtgatatcac
    13741 ctacgatggc aactttcaga ccttcaaaat gaccaaattc ttcataaatg gtcatcaaat
    13801 caagcaagca ttgggttgga tgctgtccgc ttccgtctcc tccattgata atcgaacact
    13861 ggattgtttt actttgtatc agttcgtcat aatagttctc atcaccatga cggataaccg
    13921 ctacgtctac gcctaatgct gacatcgtca gtactgtatc gtatagtgtt tccccttttt
    13981 ggacagagct tgtgcttgct tcaaattcaa tcacttctag ccctagtttt ttctccgcta
    14041 catcgaagct tttatgtgtg cgagtactat tttcgaaaaa gaggtttgta gcaaagtact
    14101 ggcgcttttg cggagtccaa ttagcacctt gcttgaattc ctgccctctg cggatcaagc
    14161 ccataacttc ttgatcagta agtgcctcaa cggtcaataa atgctttaag ctgatacgtt
    14221 ctgatttgat gatcataatg tttcctcctt agttttcttc gcttcgtgaa gtttctggta
    14281 agataagatt taaaatgatg ccaagtacag ttgctaatgc cattgccgac aatgtaaatg
    14341 tgcctacttc aaagacgagt ccgccaattc ctactactaa gataactgaa gcaatcaaca
    14401 gatttttctt tttatcaaaa tcgattttgt tctcgattaa aatcttcatt ccacttgcgg
    14461 caattactcc aaataagata aaactgattc ctgagatgac tggtccaggg atacttaaaa
    14521 tcaaagcgct taattttcct acgaatccta aaatgactgc aaatactgca gctccaccga
    14581 tcacaaacac gctatggaca cgtgtgatag ccagaacacc aatgttttca ccgtaacttg
    14641 ttacaggagg acctccgaca aaggctgcca cgatttgtgc taatccatca cctgtcaacg
    14701 tcttatgaag acccggttct tcaaagaaat tacgtttagt tagtttattc aataccataa
    14761 gatggccgat atgctctgtc atcgtcacaa aagcgatggg tgccattgtt gtgattgctc
    14821 caagatatag ctttggttca tattgtacaa agggaatctc aaagttagga acagcaaacc
    14881 aggcagcgtc tacgattggc ttagtatcca cgattccaaa cagcaaggct acgatgtatc
    14941 cgctaataat acctagtaaa atcggaatca atccgagaaa acctcttagc cacatgttga
    15001 agaaaattgt taatcctaat gtgatcagcg ctacagcaat atacttgaaa tcatacgtgc
    15061 cctgattata catagcattt gttgctgcat ttgccgcaag tcccaacccg ataaccatga
    15121 tgactggacc gacgacgatt ggaggcaaga ttttatccag ccatgctgaa ccgatctttt
    15181 tgatgatcag cgcaacaatc aaatagacta atccagttgt gactgctcct tgtgcaatcg
    15241 ctggataacc atctgttttc atcaaaagct gcatcgctgc aatgaaagcg aagctgcttc
    15301 ctaagtacgc aggtatcttt cctttagtag taaataagta caccaatgta cctagtcctg
    15361 aactgactag cgcaatacct ggatcgatac ctactaagat ggggactaac acggttgctc
    15421 cgaacatcgt gaacaagtgt tgtaaactta atcctaccca atgtcctggg gttgggcgat
    15481 cgtggatatc taacaccacg tcatcattat gaaatccttt tttctccgac atgtttctct
    15541 ctcctctaat cttcctcttt caaaatcatg atgcgatctt gtccatcaag ctcttgcatc
    15601 tctactaaaa tctcttccgc ccgagatgtt ggaatgttct tcccaacata atctgcacgg
    15661 attggcaatt ctcgatgccc acgatcgacc aggactgcta atgaaatttt tcttggtctg
    15721 ccaaaatcca ttactgcgtc cattgctgct cgaatcgttc gtcctgtata caaaacatca
    15781 tcaatcaaaa taacttcttt tccttctaag gaaactggga tgtcagaaga atgaagttcc
    15841 ggttcttctg gattttcttt tttgtcatct cggtataaag tgatatccag ttctcctaca
    15901 ggaatatcga tatcttctag ctgcttcagg cgttcggcaa ttcttgatgc aatgtagatt
    15961 cctcgtgttt tgatacctac caatacgata tcttggatac tatggtttct ctcgataatc
    16021 tcataagtaa ttcgagtcaa tgctcgcttc atggttacct gatctactac ttcttttgct
    16081 tgcattttca ttccccctac ttttttgcat aaaaaaactc ctgccctaag aggacaggag
    16141 gaatgtttag actgagcgta tactgataca ctgatagcta tactatgtca atgagcaatc
    16201 tacttatggc gcaccatacg tatccttgct cccacccttc ttagcctcac gggactaaac
    16261 ttaaaggaat tctattaagt tttatgcttt cgttgttaat tactataccg ttatctaaat
    16321 taaaatgcaa tgttttttct taatttttct aaagtttttt caaaaacttc tggaagaggt
    16381 gcttcaaaga tcatcttttc gtttgtagtc gggtgcgtaa aacctaacat ttgcgcatgg
    16441 agaaactgac cattgccttt caaagttttc ttcggtccgt atactggatc tccagctaac
    16501 ggataaccga tatacttcat atgcacacgg atctggtgtg ttcttccggt ttctagtttt
    16561 aattcaacca gagtaaatcc ttcgaaacgt tccaatacag taaaatgtgt gacagccgac
    16621 ttcccatctt ctcttatcgc ctgcattttc cggttttcct tcgaacggcc gattggcgca
    16681 ttgatggttc ctttttcgtg aggaatctct ccatggacta aagcaacata tttgcgcaaa
    16741 gaggtcttat cttttaattg ctgagctaat gattcgtgtg cctggtcatt tttggcaacc
    16801 attagcagtc cggaagtatc tttgtcgatt cggtgaacaa tacctggacg aataacgccg
    16861 ttgatcgtag aaagatcctt gatatggtac atcagcgcat tgaccattgt gccatgcgta
    16921 tgacctgctg aaggatgaac gaccattcct tgaggtttgt ttatcaccgc tacatcttca
    16981 tcttcgtaaa cgatcgtcaa cgggatattt tctgggacca catctaatgt ttctggttcg
    17041 gggatcaaga cagtaatctc gtctccttgt tttactttgt aatttgcttt gactggctgt
    17101 ccattgacag aaaccgctcc atttttcagc cacagctgaa tttgcgaacg actgtaatcg
    17161 gtgaataaat tagtcaatac tttatcgatc ctaccagatt catggtgaat cgtcgtttta
    17221 agttctgtca tttttttctt tgatcctttt catctaaaat aatataaatt aacatacaaa
    17281 tcactccaca tacaagtgtc acatctgcta cgttaaaaat cggaaagctc ataaaatctg
    17341 tctggaacat atctactaca tagccaagac gaatacggtc gataaaattg ccaatcgcac
    17401 cagctagaat caaactcaat ccaatcatga accagcgatt acctttttca cgatttttaa
    17461 tcatcaatgt gagaatcaca acgactgcaa tgagtgtcac tatagtaaag aaccacatct
    17521 tcccttctag catactccat gcggctccag tgtttcgaat atgattcagt gacaaaatac
    17581 ctggtataag atttttcgtc tctccaagcg ctagattatc agtgatccac catttgacca
    17641 actgatccac tcctactagc aaagcgctga atatccaata aataattaac aaaaaatcaa
    17701 tccttttcta tttgtttata aatatcacga ggtcgtacga tcccgagcag attaccatcg
    17761 ggattcccat ctttagtgat aagtacggct tccaaagtcc cttgctcacg aaacatcgct
    17821 tcaacctcgt aaatatgtgt tttagctgga acaaaatgat aatttgtgta ctttccgttt
    17881 tggatgatca aatccaacgc tcgtttattt gttaaatcaa ccggaccttt ttggctttct
    17941 tttgctagcc aaaacccgat attacgtaaa gtaatcaatg ctacaaattt tcctttattg
    18001 tataacggaa attgtgaata ccttttttct gctactatct ttaacagttc aagtaacgga
    18061 agatcttgtt caaaacctgt tacacgtttt gcaaatcgag gcagcacggt ttcaggctga
    18121 atcagttctt gttcgatcag ctggattcgc ttgactgtcc aatcatttgg ctctgcaatg
    18181 acaaaatcga ctgcgatccg atcatggacg atcgcattcc ttaactgagc aagctgtaaa
    18241 agatcatctt cgtattttct gatcattaat tgttttcttt gagctaggcg tctgacaagt
    18301 tcggtaaatc ccatatttcg tgcatttccc atctcgtctc tcatccattt ttcgattcga
    18361 ttgaaactga ttaaaaaaag atcagagttt gccataattt tcctccttga tcaaatatgc
    18421 ccggtttttt atatagaaaa agaacgcaat caacacaata actgcaaaaa aactgaaaaa
    18481 taaattttgt aggaaaacgg cccccaaccc agttgctaga aataaaaacg gatagatttg
    18541 aagaatcaag taattcattg tgatcacata agcaggcttt gcttctttca ctgtataaac
    18601 ggggcttttt agccaataaa aggtgacctg catcgtaaag ggcacttcca gatcaagcgt
    18661 tatcttttgt tcatgactga gaacactgcg tttttcattg ttgattctta gctgaatcga
    18721 gctacccata ccgtaaaaac cggtttttct tataaatgtc gttttcatca taccctccta
    18781 ttatgcttta gtatagctca aaatacaata aagaaaaaga atgatcctag tgtaaacaaa
    18841 attaattccc tatcgtctaa aaaaagaagc tgtagtcaaa atacagctcc ttcgatggta
    18901 gattataatc aagcttaaaa caatcccaac acttctccgt catttgtcac gtccatattc
    18961 aatgcagcag gttttttagg caaaccaggc atcgtcataa catctccagt caaggctact
    19021 aaaaatcctg cccctaattt tgggatgatt tcgcggatcg tgatggtgaa atcttccggt
    19081 gcacctagca gtgaaggatc atcagaaaat gaatactgtg tttttgccat acaaatcggc
    19141 agtttgtccc atccattctt cgtaaactct tctatttgtt tttgcgcttt tttgctgaaa
    19201 acaacaccat ttcctccgta aattttcttg acgattgttt ctgtcttttc ttgtatcgtt
    19261 tcttctaggc ggtataatgg cttgtaatta gcttcttttc tatcaatcaa tcgtactaca
    19321 gcttttgcta aatcaacgcc gccttctgct ccatctgccc atacactcgc tctcttggca
    19381 agaatcccct gattttcaca caagtgctcc aacagtgtga gttcattgtc tgtatctgtt
    19441 acgaattcat tgattgcgac gatcacaggt agctgatatt gttccatatt acgtatatgg
    19501 cgctttaaat ttgcaaaacc tactttcaaa gcatcaagat tctcattttt aagttcatct
    19561 tttttcattc cgccatgcat tttcaatgca cggacagtgg cgacaatcac cacagcatcc
    19621 ggcgcttttt tcaagttagg tactttgata tctaaaaatt tctctccgcc aagatctgca
    19681 ccaaatcctg cctctgtgac aacgtaatct gctaatctca atgctgtctt ggtagctaaa
    19741 atactattgc agccatgagc gatattggca aacggtcccc cgtggacaaa agctggtgtt
    19801 ccgtaaattg tttgtacaag attcggcttg attgcatctt tcagcagtaa tgccagagcg
    19861 ccttctgctt tcaagtctcc agcagtcaca ggctgacggt caaatgtata gccgacaacg
    19921 atccttgcaa gacgagcctt taaatcttca aggtctgccg caagacacaa gatagccatg
    19981 atttcgctgg caactgtgat atcaaaccca tcttctcttg gaacaccttg gattggccca
    20041 cccaagccaa caattacttt acgcagttca cgatcattga gatcgactac ccgtttccaa
    20101 atcactcggc gggcatcgat gttcaattca tttccttgat gaatatgatt atctaaaaga
    20161 gcagataatg cattattagc tgttgtgata gcatgcatgt caccagtaaa atgcaagttg
    20221 atatcttcca taggcaatac ctgtgcatat cctccgcctg cagcaccacc tttgattccc
    20281 atcacaggac caagtgaggg ttctcgcaat gcaataacag attttttatt gattttattg
    20341 agcgcgtctc ctaaccctac agtgatcgtt gactttcctt cgccagctgg tgttggattg
    20401 atggaagtaa ctaagatcaa atgcccctca gcgttatcct ttgatcgatt gattccagaa
    20461 aaattgattt tcgccttata tttcccatac agttccaaat cgtcttcctt aagatctaat
    20521 ttactagcca cttcaacaat cggttctagc tctgtttctt gtgaaatttg cagatcattc
    20581 ttcatttgat tacccctttt cacgaacttt attcaacaaa atatcttctt tgttaagata
    20641 tgccaattat accttttttt attcagaaaa aaaacataaa attacaggtt tttaatttat
    20701 ttgacgtact tataaatact ctttataatg aaggctatga tcagacttta gaaaggtcgt
    20761 gagtgctttg accacaggaa ttgtaaaatg gtttgacaac aaaaaaggat acggctttat
    20821 cagttatgat gacacagaag aaatttttgt acattttact gcgattgaag aagagggatt
    20881 caaaactttg gaagaaaatc aagtggtcga atttgagatc atcgaaggga atcgaggaac
    20941 acaagctgcc catgtcaaga aaggcaacgt tcagactacc gatactagta tgcatgaata
    21001 gataaacaaa tagagtgaga cgctaaacgg gatgaaccgt acgtctcact cttttctttt
    21061 cctgtaaaaa ttcgtttttt taggttctga tttttaaaat tagctttttc tttttttcgt
    21121 ataaggataa ggaattgatt ctgatttggg tccgtttttt tcaaaatgat caaaaaggac
    21181 tacttgatct ttttttcgca tcatgaaaac agatggggct ttctcatctt gcaacatcat
    21241 ttgcacccca gtattagttg gcgtcaaaga tcgaatctgt ccgacagata tttctcggtc
    21301 tttccaaaaa gcagcataac gtataatcac agagtttgat tttaaaataa aagaacgccg
    21361 caaacctaga aatagaaaaa caagaaaaat acctaaaaca aaattgcttt tccaataagg
    21421 acctgtgttt tctaaagaaa gaatcagact aaaaaacaga atgatcaagg tcaatgacca
    21481 atagatgatc gtatgtgcca gttcaggctg ccatcgaata ttttttttca ttctctaacc
    21541 tctcaccgat tcagtaatga tataatctta ccataacatt agtttgaaca aaaaggagaa
    21601 tctcttttta tgttaaaagt atacattgat gcctctacaa aaggcaaccc tggtcctagt
    21661 ggaggtggaa tcttgattgt ctatcaaggt aaacaagaac agctatctat cccgctttct
    21721 atagggacaa accatcaagc agagttcgaa gtctttttaa aaactttaga atttctaaag
    21781 aaaaacgact tacagaatga aacgattttc tgtttttcag acagcaaaac actagtttct
    21841 acggtcgaca aaaacgcaac gaagaacgag ctcttccttc cctaccttca aagaatccaa
    21901 gaacttctat ccgaattttc tttattgatc ctgcaatggt tgcccgaaaa caaaaataaa
    21961 ggagcagata atcttgcacg tcaggctctc cataaacaat taaaagaaaa aaagactctt
    22021 taaattaaaa actttaagga gtctttttat agtttctcat ttaatataaa agataattgt
    22081 gatcaaacgt aatttccaat tggtaatctc cgattttttc ttctatttga ctacgtaaga
    22141 ttctttctag ttctgtatca acaaactgga atttctcagg gaccactaca tcaaattgca
    22201 gcgtttttcc ttgatgaatt actctaaaat catgaatatt aagccctgta cctgtttcaa
    22261 gaagaatttg ctttaactgc cgcaagatat tatagtattt ttcgttatca acaggcattg
    22321 gatccaagtg acagacaagt tccacatcca aagacttttt aaagtctttt tctatcagat
    22381 caataatctt atgagccaca ttcaaattca ttgattcatc tacttcgatg tggactgatg
    22441 caaagcgttt attgggtcca tagttatgca ccaataaatc atgatagcca agtatatcat
    22501 cgcaagcatt taaatgctct tccatttctt caatctctgc tggcgttggg cgacttccta
    22561 atagttcgta tacaaaattt cgaagcatcg tcaatccact ataaaggata tataatgcta
    22621 aagcaaaacc tatatatcca tcaatacgcc atccagttac ccattcgatt gctgctgaaa
    22681 gcaatacagc cagtgtagta aagacatcat tataactatc tgttgctgta gctctcaatg
    22741 tttcagaatc aatggattgc gcaataacct gatacatttt gccttgtgca aatttcaaaa
    22801 agatggaaat gattaggaca aaaaagacga tgggtgttaa agagacattt tctggatgga
    22861 tgattttttc agcagaagac tctaaaaatt gaaatcctac ataagtaatg atgatcgaaa
    22921 caaataaccc actgatatat tcgaagcgtt cgtgtccata aggatgttcc tgatctgctg
    22981 gctttgcagc aattttaaat ccgatcaaag tcaatatgga tgaagcagta tctgatagac
    23041 tgttcactgc atcagccata atggagacac ttcctgaaaa aagcccgatc attagcttag
    23101 agacaaacaa gatcagattc gtaaccaatc ctaagatacc cgctagtact cctttttttg
    23161 tccgctgttg cggtatttgt ttcttttttt cttcttgttt cttagccaag ttttttaccc
    23221 cttctagttt gcttgttgct caggtaaaaa gatggagagg acgattgttt tcctctcctc
    23281 tcttcctttt tctatctatc gtgcgtccta ttactattaa aagatggtca ttattctggt
    23341 ttcaacggtg ttgctgcaaa taacgctttt gctagtaatg gtgttatgat tcccgcacct
    23401 attgcttctg gtatcccatt gattgctgca acccctgcta aggttttaaa taaaagagca
    23461 gagtctacac cataggcatc tgccgttgca cctgtataaa ataatcccat gaatcctaac
    23521 actaagactg tattgaccaa tgaacccagt acccctgcaa aggctgagct gatcgttatt
    23581 gatttatttt tttgataaat ccagtggaaa accaagcctg ctactaagcc aacaagtact
    23641 ctgggaacta ccgagataat cggattcgtg aacactaatg tatcaaatgg cgttgttggc
    23701 atggcatagg cacgaacaat tgtagcgatt ccccaaaaca agccaatcac catgccatct
    23761 tttgggccta atactactgc agcaataatt actgtgatat ggataattgt taaactaatg
    23821 aatcctaatg gaataaaccc cagccaagga accatcgctt gtacgatgat gattgctaac
    23881 aagatcgcac gtaagactag tcgaaaggtc ttatttctac tattcatttt ttcacccctt
    23941 tttatttata ctaatcgtaa aaatttctgt cactttcgtc aactaaagac catccgaatc
    24001 cgaccatttg attagatgcg ccatccaatt gaaaaaagta tgagggcaag acttgtttgt
    24061 cttcgccccc ataatcatgt ttcgttcaat gtttgaactt ttgtttttct gtcaaacgta
    24121 aacgccctat tgttgcgcaa cccattcttt cgcagcttta agtgcatctg gaataccagc
    24181 aggatttttc ccgccagctt gtgccatgtc tggacgtcca ccaccgccac cgcctacttt
    24241 cggtgcgatt gctttaatca agtcgccagc ttttagtcca gcttcatttg attctttcgt
    24301 cattgccgct agcagactga cttttccttc ttgttcgttt gctaaaacaa gaatatctga
    24361 aatatttttt tgtttccatt ggtcagctaa ttgacgcagc tgattcatat ctttcacttt
    24421 tacttgtgct gcaatgattg tttttccatt gatcgtttct ggatctttga atacatcacc
    24481 agcttgttga ttcgctaact tgctagctaa ttgttcattt tccttttgta gctctcttac
    24541 ttgctgttgc aattgttccg cttttgaaac aacttctttc aattgaggtg atttaacaat
    24601 tgttgctact tgttttagtt gtttttcttc ttcttcaagt agttcgtacg cttctttact
    24661 tgttacagcc tcgattcgtc gcacgcctgc accaattcct gattcagaaa cgattttgaa
    24721 gattccgatg ttttctgtat ttgctacatg tgtgcctccg cacagttcga ttgaccagcc
    24781 gccaatattt actactcgaa cgtcatgtcc gtatttttca ccaaacaaag ccattgcacc
    24841 catgtttttg gctgtatcga tatctgtttc aattgtttca actgggatag cttcccagat
    24901 tttttcgttg acgattcttt ccatttcagc taattcatct gaagtgattt gaccaaagtg
    24961 ggtaaagtcg aatcgtaaat gtccaggagc gactaaagaa ccagcttgat ttgcatgctc
    25021 gccaagtact tctttcaatg ccttgtgcag tagatgggtt gctgtatggt ttttgatgat
    25081 ccgattgcgc atcgattgat cgatcaatag atgataggtt tgtccttcac tcattggtgc
    25141 ttttacttct acggtgtgca taaattgacc attaggcgct ttttgaacat ctgtaacaac
    25201 tgctacgatt tcgcctactt cattcatgat ccatccttga tccgctactt gcccacccat
    25261 ttctgcatag aaaggtgttt gatcaaagat catttgagct tcaccttctg cgacttcatt
    25321 caaaagagac tcacctttaa cgatgaccaa taattgacta tctgattctt gttctgtgta
    25381 tccaatgaat ttactttcta ctttgatatc tgtcaataaa gcagattgta cccccattga
    25441 tttttcagta ctgcgggcag agcgagcacg ttcgcgttgt gcatccattt ctttttcaaa
    25501 accagcatga tcaactttca gtccttcatc ttctgccacc tcttccgtta attctaccgg
    25561 gaagccatat gtgtcgtata gtttgaagat gtcttttcct gctagagtcg tcccgttttc
    25621 gttttttact tcttggatca cttgatttaa gatttccaaa ccttcattga ttgtttcatt
    25681 gaaacgttct tcttctgtac ggataacttt ttcaataaat tctttttgtt gaagtacttc
    25741 aggataataa cttaccatga tttccccaac aacagggact aaacgatata agaatgcttg
    25801 ttcaatacct aattttttcc catgcatgac agcacgacga agcaaacggc gtaagacata
    25861 ccctctccct tcattcgatg gcaatgcacc gtcgccaata gcaaatgaaa gggcgcgaat
    25921 atgatctgcg attactttgt aagaaatatc tgttactggg ttttcaccat attttacttg
    25981 tttgcttagc ttttctacgg cgtggataat aggcataaac aagtctgtct caaagttcgt
    26041 tgacgcatct tgaacgattg agaccatgcg ttccaatccc atccccgtat caatgttttt
    26101 atgaggaagt ggttcaaatg tgttatcagg cttatggttg aattctgaaa atactaagtt
    26161 ccagatttct aaataacgtt cattttctcc tcctggatag ttttcaggat cgtcttctgc
    26221 taaatcgtta taagcttctc cacggtcata gaaaatttcc gtatccggac cacatgggcc
    26281 tgcaccgata tcccagaagt tgtcttctac atcaataatg tgatctagtg gcaggccaac
    26341 ttcttcatgc cagatgcgtt ttgcttctgt atcttttgga taaactgtta cataaagttt
    26401 ttctggatcg aatgccatcc attttggatc agtcaaaaat tcccatgccc agtggatggc
    26461 ttcatttttg aaatagtcac caatcgaaaa attacccagc atctcaaaca tcgtatggtg
    26521 ccgtgctgtt ttaccgacat tttcgatatc atttgttcga atagattttt gggcatttgt
    26581 gattcttgga ttttctggca caactgaacc atcaaaatat tttttcaatg ttgcaacacc
    26641 agagttgatc cacaataagg ttggatcgtt cacggggatc aaagacgcac taggttcaat
    26701 cgtatgtcct ttagattgga agaaatcaag aaacatttgt cgaacttgtg cactacttaa
    26761 ttgtttcatg tcattacctg ctttctataa attaaataca aaaaaacacc atcacagcca
    26821 aggacgaaaa ctcgcggtac caccttgatt gcaatgatgt gtaatcatta cctcttaaac
    26881 tcgataacgc tgagctgcgt cggtatttct accaaaatag aaaaagggag caatttgatt
    26941 agtgactatt cttttttcag ccaagaaaga attttctgtg agtcttaaaa aacaaatcat
    27001 atccttttcg tacttaatta gtatacgcat tctattgatc actgtcaata tctcaatttt
    27061 tattttcatt tccttttggt catactccac gatttttggt acgataagaa taaaggaatc
    27121 aatacatctc tgctattcat gacaaggagg aatatgatga aaaaaatagt tggagcggat
    27181 gaatggatac gcgcagctag ctgctttttg agatatcaag tttttgtatt agaacaaggg
    27241 attcctgcag aactagaatt cgacggaaaa gatatcccgc aacaaaccta ttatctattt
    27301 cttgatcaaa aaagaccagt agctactttg cgttatgaaa agaaaacacc tacttgcttc
    27361 aagccggaac gattttgcgt agcaaaagaa taccgaggaa aagggttagg cggtcagttg
    27421 cttgaagctg ctgaaaaacg ggcggtttca gaaggttgtc agactagtta tgtagtagct
    27481 gaaatgacgg ctgtagattt ttatgaaaaa caagggtatc atacttgcac agaacctttt
    27541 attgaagatg aagtactttg tatcggaatg aaaaaaagtc tagcttaaaa acaagagatt
    27601 gtgacacgcc ttttgtcaca acctcttgtt ttttatttat gtgaattttt cttattttta
    27661 cgagcagtac ggctttgctg tctgcgttcg attttgcgtt cttttttatt tttttctgca
    27721 attgcccaat cgattttttt cttataacca ggcttgatct ttttcttttt cttcttcaca
    27781 agaccaatca gcgtgggatc taatacatct tttgattttt ctcgtttggc acgtctatta
    27841 cgatcgtatg ttttgacgat ttccccgttt ttgatttctt ttggttcaaa tgagacgccc
    27901 aatttttcta tcgcatcaat ggcttgctca tcatttggcg aatataacgt aatggccgtt
    27961 ccttttaagc cattacgtcc agtacgtccg acacgatgga taaagaagtc tagatcttct
    28021 ggaatttctg cattaataac atgagagaca ccttcgatat cgatccctct agcagctaaa
    28081 tcagtcgcta caacatattg atactccaga ttctgtactt gtcgcatcac tcgttttctc
    28141 tctcttgggg tgatgtctcc atgaatcttt gctactttca agccttggtt tttcaagtaa
    28201 tcggcaattt catccacatg ctgttttgta ttggcaaata cgattgctaa ataaggatga
    28261 ccaattgtca gcagttgata aatcatttga ttctcatctt taccttttgt tgaaatcagc
    28321 caattttcaa tgtcttcaga aataactgct tttggtttga tttcttcaat gattggattt
    28381 tccatgtatt ttcgtaagaa tggacgaagt ttttctggaa tagtggcaga aaaaactaaa
    28441 aactgcagct tttctggtaa tcgacttgca atttggtcta cttcatttaa aaaacccata
    28501 tccaatgtca tatccgcttc atcgacaaca aatgcaaatg cggtgtgaat cttcagcgct
    28561 tgttcattca tcatgtctaa tattctacca ggcgtgccta ctacaacctg tggctgttgg
    28621 tgatgcagac gatttaattg acgctgtttg tctgtaccgc cgacaaaatt cgagacacgg
    28681 atctccggtg tagaaaattt agcgatttgt gtagctgctt catagatctg tgcagctagt
    28741 tccctacttg gagctgtaat aacaacttgt acttcctctt tttctgcatt gattttttca
    28801 aacaaaggca acaaaaacgt atgtgttttt cctgtaccag tttgagattg cccaatgata
    28861 ttttttcctt ttgcaattaa aggaatcaaa cgttcctgga cctctgtggg ctgtttgaag
    28921 ccctttgcgg ttaacgcttc attgataaac ggtttgaagc ggaatatatc aaaattattc
    28981 attcgtgcac ctcttctctt taaattatca cgactttggc attataacac agacaaagcg
    29041 tttttgctca gctaatttcg agaatcatca tcctttgttt ggccaatcgt gctaacagta
    29101 tatgtgtgac tagctttttc ataaaacaga atccgttttt ttctcaaaaa tgatagcaag
    29161 atatttaaaa taaaaatacc ttgtagtaac aagacaccga gagaaatgaa tactgcactc
    29221 atctgcaaca ccgtattcgt tgtcgaattc atgatttgct gaagcaactg ccccaaccag
    29281 cttaaaccat aaaaaagaaa gtacagatac ccgtagcgga tcatcaatgc ttttaaagaa
    29341 attcgttcgc ggtcttcttc caccaaacgg atacgcacaa tttttttgcc aagtgtccat
    29401 ccattggtta taaacggtac aatcataaag tataccccta cttctaataa aagccaccag
    29461 atatgagaag taactgcccc tgaatcgaac gcaattttaa agaaaatcgc cgctaccaaa
    29521 ttaataagag ataaaaatgt ccagtcgatc aaataagcga ccaagcgtcg agtcaatgag
    29581 acagtctttc ctttttcata agaaagttca tccattcgtc ttcttgtagg aagcataaat
    29641 gtaaataacg gcgtaacgac aaaaccaatg attccaccca atgtatttgt cactaaatca
    29701 ttcacatcaa ataagcggta tggtcttggg tagataaaat aaagtccact tagctgcgtc
    29761 aattcaaaaa atagagaaag gcagaaacta gcaatcatcg ttttcataaa agaaagccgg
    29821 aaataatatc ttaaataaat gccaaacggt atgagtaata aaacattaaa agctggttcc
    29881 aaaaatacac tctgcttcat tgccggcagg taggtactag gttcagacaa gaccaatacc
    29941 gtgttgtctc tgaaatgttg ccatgaagca aataaatgca gctccatctt tggccctgtc
    30001 atttgggcaa ccgtctcctt tgccggaagt ggtaagatca ccaaaaaata cgcacacaat
    30061 aaataaaaaa cgaaagaata caaaataact gcccttgtta ctagaaatgt tccatacttt
    30121 cgatattcat aaataaaaaa aacagatgag atcgctaagg ctaaaaatgg aaaaacaatc
    30181 agcgcaaaac gaatcggttc gatataactt gccatatgac tcctccttga ttctttgtat
    30241 ctctcttatc ttagcagtcc tttgctgttt caagcaagta acgaggctat tatttctcat
    30301 ttctttcaaa gtaaagtaag ataaacacaa agggggtgtc gagatgctga atatgtactt
    30361 tgtttttggc gtacctattt ttttactgtt cctttatgct acgatcgctt atgtaagaaa
    30421 aagaactaca attcattatt taggatttat tctcctgatt atttcgggtt tcatgctagt
    30481 cttcaatctt cagacttggc agcaagcact tcttgaaatg gataaaatga ctccacatgc
    30541 tttgtctaaa gtgcttggct accctgtcta tctgatttgg cttcccattt tcatttctgg
    30601 ttgcttggtt ttattaaata tttatcgcgg cgtacgccga atagttcagt tacgcaaatc
    30661 aaagtaaaag acagaaatcg gttgagcccg atttctgtct tttactttgc tatttcatcg
    30721 tagacaaaac gacatttacg tgatcaaaat taacgagtac ttttgacatt tcttccacgt
    30781 actcctcatt taaataaaat attttgtttg gcagagaagt gcagttctaa taaaattcga
    30841 atgttttagc tatctacgtt ttaaatagag ataaatcggg ataccagcaa gtgtcaatac
    30901 tatccccatc gaagctaaaa aggtctgagt gataagcgtg cttgatacga taaaagtccc
    30961 tccaatgatt gctactagtg gaataaacgg atacattgga actttgtatg gtcgaaacaa
    31021 atcaggttct tttttacgaa gaagaatgac accaacaaag accatcgtat aaaaaatcca
    31081 aatgacaaag atcagcatat ccgttagtgt atcaaaacca ccgatcatca tcatgccaat
    31141 agcgatgact agctctaaga tacctgcggc tacaggaact ttagttttat catgaagttt
    31201 agcaaagagc ttgctaaaag gcaggttgtt ttcttttgcc ataacataag ggagacgcat
    31261 tccagtaagg gtatagccat tgatggttcc ataaacagaa atcaagatac caactgtgac
    31321 cagtcttcca ccgaagccgc caaaaatcat ttttgcaacg tcacttgccg cattgctgtt
    31381 tcctgcgact ccgtcgatag atgcggttct taagaacacc gcatttacta ataaataaac
    31441 gatcatgatt ccgatgattc ctaaagaaat tgcttttggc aaatctttcg ccggcttttt
    31501 caattctccc gaaatatttc ctacgtgtat ccaaccgtcg tatgcgaaca tcgttgctag
    31561 caagcccgct cccaaggctg agaaaaatga aagattttct cctgcttgga tcggaaacag
    31621 ttggaagtct actccttctt gtcggaacaa accgaaaatg acgatcacaa acaaaggaat
    31681 aagcttgcag acaagcgtaa tcgactgaaa agcccctcca gctttagaac ctaaaaaatt
    31741 gatcaacagg atcgatacag cagctgtaac agctacggga acaatcattg actgggaaag
    31801 accaaataga ttgacgaact gtgtaccgaa aatgatcgac aaggctgcta catttgctgg
    31861 aaaataaatc acgacttgcg cccagcccaa taaaaaagct gctgtatttc cgtaaatccg
    31921 ttcgatatat ttaatcatcc ctcctgtttc tggaatagca gctgccagtt cagctcctgt
    31981 tagtcctgca cagacagaaa tcataccacc gagaaaccaa gaaaacatat gcaaactggc
    32041 ggatcctgta acttctgcta cgcttgcggc tttaaaaaat acccctgcac cgatcactgt
    32101 gcccataaca gtcgataaag cagtaaaaaa tcccatcgta cgttttaatt cattttctat
    32161 ttttacttct tttactggcg tggcttgata ttcttccatc ccttgtcccc tttcatacca
    32221 tagccaatcc caaaagtata aaaaactttt tggatgtggt tcttgtttta taccgtctct
    32281 accaattttt ctttcagttt ttccaacata tcctttgtca tcacatctaa gtcgtatttc
    32341 ggcgaaaagc cccattcctc ttgcgcacaa gaaacatcta gtgaatttgg ccaagattct
    32401 gctattgact gacgtacagg atccacatca taatccattt caaaatctgg tatgattttt
    32461 tgaatagatg ctttgatctc ttccgggtca aaagacatcg ccgatatatt gaaggcattt
    32521 cgatgaacca atcgatcagg atctgcattc atgagtttta taatggcatc gatcgcatct
    32581 ggcatataca tcatatccat tgatgtacct tgatcaatga aactagtgta ctttccttct
    32641 ttgattgctg aataataaat atcgactgcg tagtctgtcg taccgcctcc tggcaaggtt
    32701 gcataagaaa tcaatccagg gaaacggaca ccacgagtat ctacgccata tttcacataa
    32761 taataatcac atagtagttc tccagctacc ttcgttaccc catacatggt tgtaggacgc
    32821 tgaagagtat cttgtggggt atgatctttt ggagtatttg gaccaaacgc gccgattgaa
    32881 cttggcgtaa aaaatttcag ctggaattct ctagcgactt ccaatgcatt gaccagccca
    32941 gtcacgttca aattccaggc taattgtggt tttgcttctg caacagctga caaaagagca
    33001 gccaaatgaa tcaatgtgtc cacttgatat tgttctgcta tgctgcgcat tttttcgtaa
    33061 tccattacat ctaggatctc aaagatatcg tttttctcaa tgacttcagg catgcgtata
    33121 tctgtcgcaa tgacattttc actgcctagt tcttgacgta gccgtgtgat caactctgaa
    33181 ccaatttgtc ctaaacatcc tgtcaccaat acttttttca tgatgatcct ccctgatctt
    33241 ttttactact atcttcagat gattaacaat atatgcgtga acatgttctc taattttaaa
    33301 tagacgaaaa aactaaattt agttaaataa tttccatttc ttttcctatt ttttgataaa
    33361 ctgctactgc ttcatcaagc atttccctcg tatgttccgc agtcggcata tttctgatcc
    33421 tgcctgtccc aagaggcaca gttggataaa cgattggttt agcataaatc ccatgttcga
    33481 tcagcttctt agagaaagtc tgagtcactt tttcgtctcc caaaatgact ggagtaatag
    33541 gtgtttcaga cataccgatg ttatagccta cttttttcaa ttcttcttta aaataattag
    33601 cattttccca aactttttcg acaagttccg gatgttcttg catcaaagta atcgaagcca
    33661 atgcagctgc agctgcacct ggagttagcg aagtagaaaa taggaatggc cgacttctag
    33721 ctttcaacca gtcgattagt gttttcgatc cagctacata acccccaaca acacctatag
    33781 ctttcgataa cgtgcccatt tgcatatcga ttttgtcgct taatccaaaa tgcttgacgg
    33841 ttcctgcacc ttttccagtt acgcctgagc cgtgcgcatc atcgacgtag gtgatcagtc
    33901 catatttttc aacaatcgga ataatttccg gcaatcgtgc aatgtctcca tccatggaga
    33961 aaacaccatc tgtgatatac atgatttttt tatatttctt actttctatc gcttcctttg
    34021 cttttttctc aaggtctttc atatcttgat gtttaattcg gatgattttt gctccggata
    34081 agcggcaacc gtcaataata gatgcatggt ttaattcatc cgataagata gcatcttctt
    34141 ttgtcattac tgcagagata gcgcccatat tgcagttgaa tccagattga aaggcgattg
    34201 ctgcttctgt ccctttgaat tctgcgattt tttcttctaa ttgctgatgg attttcagcg
    34261 aaccattgat cgttcggact gctcctgctc ctacaccata agtttcggtt gcttcaatac
    34321 atgctttttt caattcttcg cgattggcaa atccaagata attgttggat gctagattga
    34381 tcatcttttt tccgtctacg atgatacaag caccattttc actttctagt atgtcgatcg
    34441 tattgtataa ccccttttct ttcagttgat ccaattcttt ctctaaatac attgttagtt
    34501 ctttggaaga cattgtctcc acctccatat tcatctgtct tgcagaacat accaaacata
    34561 tatcgtgtca ttcatctaca aagcaagtat gctatatttt tgataacgct tacactgatt
    34621 atgagaatta tttaaccaca ttttttattt acagtatctt tttactttca aatgacttat
    34681 atagtatgaa caattacctt ttagataatg atctatttta ctctacctgg gcagataaat
    34741 acaacacaac cattgataga agccctttcg ttaaaatcaa ttgtattctg aatatttaat
    34801 ttatttattg catagaccaa taaaaataca taaaatttcc atcttcgttt atcagcaatt
    34861 tttaatttta ctctctgaat atttcttaaa agttttactt gttgaattcg aaaaaaggac
    34921 tgtgacaaaa gtcttgtcac agtcctattc ttccgaata
//
LOCUS       DAYYGO010000015        34903 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000015 DAYYGO010000000
VERSION     DAYYGO010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.015"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            122..574
                     /locus_tag="ACHA5T_000642"
     CDS             122..574
                     /locus_tag="ACHA5T_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8886244.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(698..1549)
                     /locus_tag="ACHA5T_000643"
     CDS             complement(698..1549)
                     /locus_tag="ACHA5T_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFM8886245.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(1562..2347)
                     /locus_tag="ACHA5T_000644"
     CDS             complement(1562..2347)
                     /locus_tag="ACHA5T_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8886246.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            complement(2699..3640)
                     /gene="ribF"
                     /locus_tag="ACHA5T_000645"
     CDS             complement(2699..3640)
                     /gene="ribF"
                     /locus_tag="ACHA5T_000645"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFM8886247.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            complement(3644..4567)
                     /gene="truB"
                     /locus_tag="ACHA5T_000646"
     CDS             complement(3644..4567)
                     /gene="truB"
                     /locus_tag="ACHA5T_000646"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFM8886248.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            complement(4935..7297)
                     /locus_tag="ACHA5T_000647"
                     /pseudo
     CDS             complement(4935..7297)
                     /locus_tag="ACHA5T_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
     gene            7462..7918
                     /locus_tag="ACHA5T_000648"
                     /pseudo
     CDS             7462..7918
                     /locus_tag="ACHA5T_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            complement(8075..8422)
                     /gene="rbfA"
                     /locus_tag="ACHA5T_000649"
     CDS             complement(8075..8422)
                     /gene="rbfA"
                     /locus_tag="ACHA5T_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFM8886249.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(8449..10755)
                     /gene="infB"
                     /locus_tag="ACHA5T_000650"
     CDS             complement(8449..10755)
                     /gene="infB"
                     /locus_tag="ACHA5T_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFM8886250.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(10768..11079)
                     /locus_tag="ACHA5T_000651"
     CDS             complement(10768..11079)
                     /locus_tag="ACHA5T_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFM8886251.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(11076..11369)
                     /locus_tag="ACHA5T_000652"
     CDS             complement(11076..11369)
                     /locus_tag="ACHA5T_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFM8886252.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(11391..12566)
                     /gene="nusA"
                     /locus_tag="ACHA5T_000653"
     CDS             complement(11391..12566)
                     /gene="nusA"
                     /locus_tag="ACHA5T_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFM8886253.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(12589..13062)
                     /gene="rimP"
                     /locus_tag="ACHA5T_000654"
     CDS             complement(12589..13062)
                     /gene="rimP"
                     /locus_tag="ACHA5T_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFM8886254.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(13207..17565)
                     /locus_tag="ACHA5T_000655"
     CDS             complement(13207..17565)
                     /locus_tag="ACHA5T_000655"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFM8886255.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            complement(17777..19486)
                     /locus_tag="ACHA5T_000656"
     CDS             complement(17777..19486)
                     /locus_tag="ACHA5T_000656"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFM8886256.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            complement(19554..20822)
                     /gene="rseP"
                     /locus_tag="ACHA5T_000657"
     CDS             complement(19554..20822)
                     /gene="rseP"
                     /locus_tag="ACHA5T_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFM8886257.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(20983..21783)
                     /locus_tag="ACHA5T_000658"
     CDS             complement(20983..21783)
                     /locus_tag="ACHA5T_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFM8886258.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(21780..22592)
                     /locus_tag="ACHA5T_000659"
     CDS             complement(21780..22592)
                     /locus_tag="ACHA5T_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFM8886259.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(22787..23344)
                     /gene="frr"
                     /locus_tag="ACHA5T_000660"
     CDS             complement(22787..23344)
                     /gene="frr"
                     /locus_tag="ACHA5T_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFM8886260.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(23347..24069)
                     /gene="pyrH"
                     /locus_tag="ACHA5T_000661"
     CDS             complement(23347..24069)
                     /gene="pyrH"
                     /locus_tag="ACHA5T_000661"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFM8886261.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(24205..25086)
                     /gene="tsf"
                     /locus_tag="ACHA5T_000662"
     CDS             complement(24205..25086)
                     /gene="tsf"
                     /locus_tag="ACHA5T_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFM8886262.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(25185..25967)
                     /gene="rpsB"
                     /locus_tag="ACHA5T_000663"
     CDS             complement(25185..25967)
                     /gene="rpsB"
                     /locus_tag="ACHA5T_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFM8886263.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            26326..26805
                     /locus_tag="ACHA5T_000664"
     CDS             26326..26805
                     /locus_tag="ACHA5T_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM8886264.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            27022..28113
                     /gene="phnW"
                     /locus_tag="ACHA5T_000665"
     CDS             27022..28113
                     /gene="phnW"
                     /locus_tag="ACHA5T_000665"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HFM8886265.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            28106..28234
                     /locus_tag="ACHA5T_000666"
     CDS             28106..28234
                     /locus_tag="ACHA5T_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFM8886266.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            28238..28783
                     /locus_tag="ACHA5T_000667"
     CDS             28238..28783
                     /locus_tag="ACHA5T_000667"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFM8886267.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(29237..30928)
                     /gene="argS"
                     /locus_tag="ACHA5T_000668"
     CDS             complement(29237..30928)
                     /gene="argS"
                     /locus_tag="ACHA5T_000668"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFM8886268.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(31348..32295)
                     /gene="arcC"
                     /locus_tag="ACHA5T_000669"
     CDS             complement(31348..32295)
                     /gene="arcC"
                     /locus_tag="ACHA5T_000669"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFM8886269.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(32410..33429)
                     /gene="argF"
                     /locus_tag="ACHA5T_000670"
     CDS             complement(32410..33429)
                     /gene="argF"
                     /locus_tag="ACHA5T_000670"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFM8886270.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(33520..34749)
                     /gene="arcA"
                     /locus_tag="ACHA5T_000671"
     CDS             complement(33520..34749)
                     /gene="arcA"
                     /locus_tag="ACHA5T_000671"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFM8886271.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         9410 a         7489 c         6162 g        11842 t
ORIGIN      
        1 aaagttagaa ttttctgact tgatttttac aatgacctta attaaaatcg ttctttctac
       61 atttcgtgat taagcaaata tcaactaagt cagtagaacc attcacatag gagggtgaac
      121 attgaaagat ttttactcag taaatgaatt agccgaacaa ttaggtgtta ctacacgttc
      181 tatcagaaat tatcttcatg aaggaaaact aaaaggaaca aaggttggcg ggcaatggaa
      241 attttctgaa cgtaatcttt ttgagttcct atacggagat caagcagagg aagcagcaaa
      301 agaaatgcag cgattcatgc taaatgcacc aatcacgatg cgtttcaacc ttcaatatcg
      361 tgattttaca gcaatcaatc aattccgaga acaattagtc cagtaccata acgatgttta
      421 cgcaaataaa aaagaccgtc ttttacaata cgatctttat aaagacaatc atgcggagat
      481 actaatcggt ggtaacttca attatgtcgt aaactttagt caatggatca atgaaaaatt
      541 attgatccag acagatattt cccttgtatc ttaaacaatt aaaaagcgct agaaattcca
      601 gagatgttgg aaattctagc gctatttttt tataagtact tactgtttgg ttcaaacaaa
      661 tattttttta ataaatgaaa ttttaccatc tttatcctca aataaagacc gtgaccatgc
      721 ttcctactac gatcagcaat aatccgataa tcgttaattt tttttctttt ggtgtcttat
      781 tttcatgcag gatgtacata ccgcctaatg tagagataac tacgtttagc tgtgtaatga
      841 tatatgcagt agcaacccca ttttcatttg ctgaaaaaat ataagccaat gctgacacac
      901 taaagatggc tccaacgatc gttgctttcc aactcttgtc ttcagaaaat gcggttggtt
      961 tacggaaaat cacataaatc actgccccta aaaagacacc gatcatttgc ggaaagaaaa
     1021 tggaaagtcc gcttgcatca actagtttag gcaatgcact gtatacccaa taaccaatac
     1081 ttgttgatgc taatagacta attccttttg tgataccgcc tttttttcca ggatcatcac
     1141 tgattgccgt taaagcagaa ccaaatacaa gaagaatgat tgctgctgct ccaatgattt
     1201 tccccatact tccagaccat tctccaaaag cgaagacacc aatcaacgag gttccgacta
     1261 actgtaatgc agtcgaaatt ggcatcgttt tagaaacacc aatcaagttg aaagatgtat
     1321 actgtcctgt ttgtccaata acccaaaatg caccagaaag taaactaatc aaaaatgtag
     1381 tcactgaaat accagctgga gaagcgaata attgaacgag taagccgaca agcaccgctc
     1441 caataccagt tcctaatatc tcatttgttg gtctgccacc aatctttttc aaaatcagtg
     1501 gctggatccc ccagccaact gccgggatca atgcaatcaa taaacccatt tttattcacc
     1561 cttatccttt tccaccttgg aaagaaggat ataaagtcat cccaccatct gcaaatagtg
     1621 taatacctgt aacataactg gattcgtctg aagccaacca tgcagctgtc gccgctattt
     1681 cttttggttc acctacgcgt cctagtggga tcatactttc caagagttct ttttgttcag
     1741 gatcatcaaa ttttgccgca ttaataggtg tacggatagc accagggcct atactattga
     1801 ttctgattcc cttcggtgca tactccattg ctaccgtttc ggtaaataat tttaccccgc
     1861 cttttgaagc agcgtaatga gcaaacgttg gccatgggat ctgttcatga acagaagaca
     1921 tattgatgat atttcctttt ttattattct ctgtaaagta atttaaagcc gctttcgttc
     1981 cgataaaaac gccagtcaaa ttgactttta acactctttc ccaatcttca aggctaagtt
     2041 catgggttgg atgctgattt tccatcccag cattattaac ccaaacgtcc agtgtcccaa
     2101 aattttctaa agccgtatca agtaaacgct gtattccttc ttcagaactg acatctgctt
     2161 gtacagagac tgcttttccg cctgcctctt cgattgcttt gactgctttt tctgctcctt
     2221 ctttatccga gttatagttg acaactacat tcattttttc tttcccgaat cgttgagcaa
     2281 tcgctgttcc aatccccttt gagccaccag ttactactgc gactttgttt tctaattcct
     2341 tgtacatagt acccactcct ttttcatacc taaacctgtt attacggata acttttattt
     2401 ccgccttaaa gtgagtgtac accaagatat atagtctaaa gcaaatgata cgctctagca
     2461 atcgttttca tgaattgcta gtgtcctttt atttctataa agaaagaggt tgtgacagaa
     2521 gtggtcagct tcaagaaatt tcggcttatt tccgaagaag ctacttctga aaaaccattt
     2581 atttggaaaa aaaggctgtg acaagacttt tgtcacagcc cctttcctgg gcttatttat
     2641 cacccatttt attgtaattg ctagttatac ataaacaaga gattttatgc ataatgtttt
     2701 attgctgaga aaaatattca gctgttgctt gttcatcctc cttgagttgt tcaatcaatt
     2761 cctctacact agaaaatttc acttgatctc ttaaaaattg attccagcgt accgcgacat
     2821 gttcaccata gatatcttga ttaaagtcca aaatatacaa ctctacagtc agctcccgtc
     2881 cttcaccgaa tgtatcatta tgtccaattg aacccatggc tggataccat gtttccccaa
     2941 tttttatttc cgacacataa acaccttctt taggcaaatg aaccgtgctc tttactttga
     3001 catttgctgt agggaatcct agtagtctgc ctcgtgcatc gccatggaca actacccctt
     3061 ctacctcata tgtgcaacct aatagttttg ctgcttcttt tacttgtccg ctgtctagta
     3121 atccacgaat acgggtagag ctgatttttt caccttgttc ttcttcttta gaaactgtga
     3181 cgattttgaa tcttcctttg gcatattcag gaaaatgagc gacatctgca atgtcttttg
     3241 gtccatacgt ataatcaaaa ccggaaacag catattttgc atgtaaccct acgatgtatt
     3301 gatcaacaaa ttcctgcggt gctaaatgag caaaagaaga agtaaaatca acttcatata
     3361 aataatctat acccaaagcg gccatttttt cttctttttg cttaaggcta gtcaaatatt
     3421 tcacgctgct aggatcgatc tttttaaaga caatagatgg atgctgattg aaagtcatca
     3481 atgctaattt caatccttct ttttgtgcaa tttccatacc tgtctttatg actttctggt
     3541 gtccgatatg cacgccgtcg aaaaatccca gcactaaaac gacatcgtct tctggaattt
     3601 gttctgattg gtaaggatgt ctgatcttga tgatattcat tcgttattcc tcatttctca
     3661 atactttact aggttttaat ttgttttttt ctttcgggtt tggctgataa atactgacaa
     3721 cttttccttt ataaaacaaa gcaatttctt ctgacggcat ttctgggagc gcaaatacag
     3781 tataatcaag tctcataccg ttttttactt tctgccatac ttcatcagta atatctactc
     3841 gtttgaaccg ttccactcct ttttctattg gcagcaaaca atcatcgatt gtcccatttt
     3901 tcacagcttg agctacttct tctaatgtca gagcatcctt ctgggacata ccggcacttg
     3961 ctgttcgggt cagatcagac atgtgcgcag ggtaaccaag tttcttgcct gtatctacag
     4021 ctaatgtgcg gacataggtt cctttcccgc attctacttc gaagcgccaa gaaatggttc
     4081 ctgtcacttc gtctcttgtc aattcacttg tccggataaa acggtaaatc tgagcctttc
     4141 taacaggacg ctccactgtt tcattattgc gggcatattc atataaccgc ttaccgttga
     4201 cttttaccgc tgagtacatt ggtggtatct gggtgatctc accaataaat gaagccatca
     4261 cttcatctat ttttttttca gataaagaca tatctactgg ttttgtttct acccgttctc
     4321 cggatcgatc ttccgtcgtt gtactgaaac ctagagttat ctcgcctaca tagattttcc
     4381 cagaatctgt caaatattcg ataacttttg tggcttttcc aatacaaatg ggcaacacac
     4441 cgtctacgtc tgggtcaagc gtacccccat gtccgatttt tttcgtatgt aagatttttc
     4501 ttaatttgaa tacgcaatca tgacttgtca ttccgcgttc tttccataaa ggtaataacc
     4561 cgtccatgtc ttttctccat ttcatttttg atttaactga attattataa cacattgcat
     4621 caattactga cgaatttgac atcataatga acggttttta tttttcggca ataaaaacgt
     4681 aacaaaattc atgctcacgt aaatagaaat catgttatgc ttttttaaag gagggaatag
     4741 aatggaagat tttttctttg caccatttga ttggatttat gggtggctta caggtaaata
     4801 gtttggtttt cacttcccaa aaacgaaaaa aaagactggc cattcttcca gtcttttttc
     4861 cgtttttttt atttattttt tcagtttctt aatcaatgta aaaaattcta ttttgcggga
     4921 tcgattttat cattttattg gtcaagattt ttccatttcc aatccaatac ttctggcaga
     4981 tcaataccat tttcacgaat atagttttga tgttcttgta atttctgatc catttttttc
     5041 gcaaaatctt ctgcttgact tcctttgatt tttaaagctg cgtcttttgc caaatgataa
     5101 cgatcaagtt catttactac acgcatgtca aatggtgtcg tgatatctcc attttctttg
     5161 tagccatgaa tcattaaatg atggttatga cgatcaaaga aaatcgtgcg gaccaatgtt
     5221 tcataaccgt ggaaagcaaa taagatcggt ttgtcttttg taaaatattg atcaaattct
     5281 tcatcgctca acccacgcgg atcttgactt ggatgacgta atttcaataa atctacgaca
     5341 ttgatgaacc gaatttttag ttttgggaat tcttccagca gcaaagttac agctgctaat
     5401 gcttctaagt tagattccgt tcctgcagca gcaatgacca attcaggttc ctcctctttt
     5461 gctgtcgaag cccaatcaat gattttcaag ccttcattca ctaagacggc tgcttcttct
     5521 gctgagtaaa attgagcacg cggatgtttg gaagagatga tcaaattgat tttttctgac
     5581 gagcggaatg ctttatccat gactgcgagc aatgtatttg catccgcagg cagatattca
     5641 cggatgaact ccgccttctt ttctgccaaa tgtgtcaata ttcctggatc ttggtggcta
     5701 tatccattgt gatcttgctg gaagactgtt gagcttgcaa tgagatttag cgaaggataa
     5761 tcatttcgcc aagaaaggtc atgtgatttc cgcatccatt tgaagtgctg agtcaacata
     5821 gagtcaacca ctcgcaagaa tgattcgtaa cttgcaaaaa accatgccgt ccagtcagaa
     5881 cgtacccttc taagaaccct tcatcttggt gttccgataa ttgactgtcg atgacgcgac
     5941 cagatggcga aagccattca tcattttctg gttcgatttt ttccatccat tggcgatttg
     6001 tcgttttgaa aacttgattc aatcggtttg attttgtttc atctggtcca aatatacgga
     6061 aattttctgg atttttctca atgatctcgc gaacgaattt accaagttcg gtcatatctt
     6121 ctgctttaac ggagcctggt ttttcaaatt gcaaagtata ttttttccaa tctggcataa
     6181 taagcggtct tggatcgatt ccaccattgg tgataggatt ttttgccatt ctttggtcac
     6241 cagtaggcgc gatttctctg atttcttcta aaatacgtcc ttgtgcatca aataattttt
     6301 ctggttcata ggatttcagc caacggagta aagcatctgc atgatccata tgttcttgat
     6361 ctacaggaat cggtacttgg tgtgctcgga aggttccttc aattggttcc ccgtcccatt
     6421 cttttggacc ggtccatcct tttggtgtgc gtacgatcaa tactggccat tttggcatca
     6481 cgacatctgt tgctgtcttc aggcgagctt ctttttggat ggatttaatt ttctcgattg
     6541 cttgatccat tttttcagcc ataacaggat ttaatttttc tgaatcattt ccttcaataa
     6601 agaaaggttc ccagccaagt ccgttaaagt aatttgctaa ttctccatct gatttacgtg
     6661 ccaagatcgt tgggttggct atttttgcac catttaaatg aaggatcggt aaaacagctc
     6721 catctatcac tggattgata aagacatttg agaaccagct tccagctaaa ggaccggttt
     6781 ctgcttcacc atcaccaatc acgacagcag agattacttc aggattgtct aatacagctc
     6841 cgactgcatg tgaaagagag tatccaagct ctccgccttc atggatagaa cctggtgttt
     6901 ctggtgctgc atgagaagca atgcctccag gaaatgaaaa tcgtttgaat aatttttgca
     6961 ttcctgtctc atcttccgtt acttctggat agatctctgt gtaacttcca tctaaataag
     7021 cattcgatac catgacttgt ccgccgtgtc ctggcccttc aatataaaac atgttcaaat
     7081 catatttatt gatgactcgg ttcaaatgga cataaataaa attttggcca ggaatcgttc
     7141 cccaatgacc gattggatgt tttttgacat cttcgggttt cagtgtacgt ctcagtaagg
     7201 ggttatcttt aagataaagt tgcccaacag aaagataatt agcagcacgc caccaagccg
     7261 tcattttatc aaagtattct ttactagaat aatccataca taacactcct tttcattatt
     7321 actcttattc tagcgcagat actcccaatt gaaacctaat aagcacttct gttcatttgt
     7381 ttggatttta tatctagcca aatgaacagg tataaaaaac cacgaaattt ttcctttcgc
     7441 tgacgttttt tacattgatc caaaaaactt gtcgttatca caatggctgt tccgcagttc
     7501 atttcgctcc tcatcatgca taatttgctg aatgacgctg ggccagttaa cacattgctg
     7561 gaaaactggg gctggatcga gcaattcctt tcctgattga tccgttttat gtaaaagtct
     7621 ctgttatctt tgtcaatatg tgaattggta tcccagctac tatgctaatt tctacaggga
     7681 ttatccaaaa tttggatcgt gaccaaatcg aagcggcgta atcggaaata tcaataactt
     7741 caacgtgatt tttcttttaa ctgtcaacgg accgacaaac tcaaactttt atggtgcagg
     7801 ttccactgat cttctagtta cttggctata cctgactgtc actaaaatag attacaatct
     7861 agcttccgtt atcggtatct tgatctttat ctatatgcaa aaatttatat actactagaa
     7921 aaaagaaagt cgaatttttt acttttcttt ttctcttttt tggttagctg acacgccgca
     7981 agactaaagt catggaactc ttaggtaata cgtcttaatg aaaacaaaaa aatcccgcaa
     8041 cccctgattt cacaggattt gcgggagttt ttgattagtc tttgttcaag tcgcgaagca
     8101 attcatcgat atggttgcca tagacaactg attcatcaag ttcaaagaac aattcaggtg
     8161 tcttataaat acttagattg tgccctaact ctcgacgaat caatcctgat gctttattca
     8221 atccatcttg tgctgttttc ttatctgatg ctaagtcaga taaaatgctg taatagatcg
     8281 ttgcttgctg taagtccccc gtaactttca catctgtgat cgttacgttc tccacacgag
     8341 ggtcacggac ctttttgcgc aaaatatcat tgacttcttt taagatttct tgaccgactc
     8401 ggcggtcacg gtagttagcc ataatgattg gcctcctttt atcagttttt agtcattttt
     8461 gacttcttcc atgataaatc cttcaattac gtcatctact ttgatgtcat tgaatttttc
     8521 aatcattgca ccacattcat aacccatttt gacttctttc acgtcatctt tgaatcgttt
     8581 cagacttgca agttggcctt catagattac gattccgtca cggattacac ggacaccgct
     8641 atctctacga atatatcctt ctgttacata ggcacctgca atcgtaccaa ctttagaaac
     8701 tttgaaggtt tcacgaacga tcatttgacc ggtgattttt tcttcaaatt cagggtctag
     8761 catccctttc atcgcagttt cgatttcttc gatggcttta tagatgatac ggtgaagacg
     8821 aatatcgact tcttcagtat cagcttgcac ttttgcctgc ggtgtaggac gaacgttgaa
     8881 accgatgata atagcgttgc tggctgctgc taaggttaca tcactttcat tgattgctcc
     8941 gacagctgaa tggacgattt ttacacggac accttccaca tcaatcttct tcaatgatgc
     9001 agccagtgct tctgctgaac cttgtacatc tgctttgatg attacgttaa cttctttcaa
     9061 ttcgccttct ttcaggcttt caaataagtt atccaaagtc acacggcttg tggcagcacg
     9121 gttttctaat aatgcacgtt tcgcgcgttc ttcacccgct gcacgtgctg tcttttcgtc
     9181 ttcgaagact acgaaacggt cacctgcttg aggtacatca ttcaagccag tgatttctac
     9241 tggcgttgca ggtcctgctt ctttatcgcg acggccaaga tcgtttgtca ttacacggac
     9301 acgtccgtaa gtattaccga caacgattgg gtcaccaaca tgaagtgttc cttcttggac
     9361 aagtaaggtt gacacaggac ctttaccttt atcaagacgt gcttcgatca ctgttccgat
     9421 tgcgcgttgt gttggatctg cttttaaatc ttctacttct gcgactaaca ggatcatttc
     9481 cagcaattct tcgatgtttt ggccgaattt cgctgagatc tcaacaaaga tcgtttctcc
     9541 accccatgct tcaggaatta actcatactc acttaattct tgcataacat gttgagggtt
     9601 tgcacctggt ttatcaattt tatttacagc gacgatgatt ggaacgcctg cagctttggc
     9661 atggttgatt gcttcaactg tttgtggcat gacgccatca tctgcagcaa caactaagat
     9721 cgtgatatct gtgatacttg ctccacgtgc acgcatactt gtaaaggccg catgtcctgg
     9781 tgtatccaag aaagtgattg gtttaccatc aatatcgatc tggtaggcac cgatatgctg
     9841 tgtgattccg ccggcttcgc cgcttgttac acgtgaatgg cgtaatgtat ccaataaagt
     9901 tgttttccca tggtctacgt gtcccataat cgttacgact ggcggacgtg agactaattt
     9961 atctggatcg acttcttctg cctcgaagaa tttatcgata tcagcaatgt caacttggac
    10021 tttttcttgt ggttccatgc catagtccgt cgctaacagt tcaatcgtat ctttatccag
    10081 tgcttggttt tgattgacca tgacaccaag cataaataat tttttaatga tttctgctgg
    10141 ctcacgatga atttttttcg caatatctgc tacgttcata ccttcagtat actctaatac
    10201 atctggcaat tcacggaatt tgcgtggtgg tacagctggt ttagttgatt gttgctgttt
    10261 gcctttttta ccttttttat tataacgatt acggttatta ttattaaatt tgccgcgatt
    10321 gttattgttt ggtttgttgt tattgttagt acggttttga ttgccgcctt ggctcgttcc
    10381 ttgacgattt tggctgctgg tatttcccgt attattttgg tttgttgttg gtttttttcc
    10441 ttgattcact tgaccgctcc cttgaccgcg atcttgataa ctgcggttat ttttattttt
    10501 ttgttgattg ttcgttgttt cttttttacg ttgatcgttc ggctgttgat tggctggttt
    10561 tctgcttgct tgttgtgcag cttgttgttt cgtgcttggg tttttgaacg cttgttgcaa
    10621 tttcttctca tcattgctag taatcgcacc catatggttt ttcacatcga tgccaagttg
    10681 ttgtgctttt tccacgacgt ctttactaga ttttttgagt tcttttgcta attcatagat
    10741 tcttttgttg cccatgcaat caccttccta accttcaatt agctccttgg tcttcttcgc
    10801 aaatccggca tccattattc cgaatacctt gcgaggtttt ccgatcatct gcgtgatttc
    10861 tgcttcagaa aatagctcaa agcaaggaac ttcataatat gaactcttat cttttatctt
    10921 ttttcttgta ttctcgcttg catcggttgc cacaaacacc aattttgctt tttgtttgcg
    10981 gatttcagca atggtcagtt cttcacccgt gattagtttt cccgcgcgca tcgctagacc
    11041 gatgagattc atggcttttt gccgattaac tgtattcatt ttccaaataa ctctttacgt
    11101 gctttttggt gagtgacata gtcaagaagt tcttgataaa actcatctgt taatgttgtt
    11161 tcgagtactc gatcgagaat ttttttatcc caagcttgtt gtacttcttc cggctcaagt
    11221 gagacatacg ctcctcgtcc aggcattttt ccagttggat cgatcgatac ttccccttct
    11281 tttgaacggg tgattcggac catctctttt ttaggtttca tttcacctga aaccacggat
    11341 ttgcgtaaag gaattttcct ttgtttcatc tctttgcctc cttactaaag ttaagcttct
    11401 ggtttttctt cgaccgtttc attgttgaat gcaattgttt catattcatc gtctgttaaa
    11461 tctgactgaa cgattgcttc atcatgcagt tcttctgttt ctttttgttg ttcttgattt
    11521 tcataaaatt ccgtcatgtc agattctgat ttgatatcga ttttatggtt agtcaattta
    11581 gcagctaaac gtgcgttttg tccgcgttta ccgattgcta gcgataattg gtagtctgga
    11641 acgacaactg tgcaggcctt tggatttgtc tcatcgaaga tcacatccat gacttgtgca
    11701 ggattcaatg catttgcaat gaatacagct ggatcttcat tccattcgac gatatccatg
    11761 ttttcgcctt ttaattcatt gacgattgct tgaactcgtt gtccctttgg cccgacacat
    11821 gtaccgactg catcgatatt tggatcattt gagcgaactg ccacttttga acgatcgcca
    11881 gcttcacggg cgatgctgac aatttcaacg attccatcat agacttctgg tacttcttgc
    11941 tcaaataagc ggcgcaatag gtctggatgg ctgcggctaa caaatacttg cggaccttta
    12001 gacgtatttt ctactcggga aacataaact ttgattcgat catgtggttg ataaaattca
    12061 tttggcattt gatcttgttt agaaagaact gcctcaattt taccaagatt gacataaata
    12121 taacgtttat cctggcgttc gacgattcct tgcatgatgt ctttttcata agcactgaat
    12181 tcgttataaa taatcgttct ttctgcttca cgtacacgct gtaaaataac ttgtttagcc
    12241 gtttgcgcag cgatccgccc aaaatctttt ggtgttactt caaaacggat cgtatcaccg
    12301 atctcatatg ctggattgat taaaagtgca tctttcaatg agacttccaa ctgtgaatcc
    12361 atcacttctt ccgttacttc ttttaccgca tagacatgga tctttccttt tttctgatca
    12421 aattccactt cgacgttttg tgcttggcca tagtgacgtt tgtatgcaga aaccaatgct
    12481 gcttctaaag catcgatcac gatttctttt gagatccctt tttcagcttc taaagcgtct
    12541 aacgcattta acatttcttt actcattttt tctgattcct ctccgtgatt aaaattggat
    12601 agctaagcga gcttttgcaa tattttttcg gtcaaaagtc aattcttttt ctcgtgtttt
    12661 tatgcgtatt ttcaatgtaa gctgttcagc atcaaatgat tgaagaaatc cttcatattg
    12721 tttttcgcca tctactggtt ggtataagga gacatggaca taatcaccaa gtgccttttc
    12781 atagtcgctt tcttttttca atggccgctc tgctcccggg gaggatacct ccaggaaata
    12841 agcttgagga atcggatctg gatctgctgc atcaagtttt tcactcagtt tctcactgac
    12901 aaaggcgcat tcttcgatat cgataccgcc ttctttgtca ataaatactc gtaagaacca
    12961 gctttttcct tcttttacaa attcgacttc gacaagttca aaattttgtt catctaaaat
    13021 cggtgtcacc atttctgtga ctgtttcaac gacgctactc aaaagtttcg cctccttaaa
    13081 aaattaagtg cgtaattcca ttaaaaagag tgagcgaaaa atccgctcac tcacagttag
    13141 tatcattacc tataagaact ataccatact ctgcttaaaa ttacaagcag acaggaaaac
    13201 agatgcttat agcatatcga ataacgataa ttgattttca tctggtagat ctttcaatac
    13261 gccattgtcg ttcatatatt cgatcaatgt ttttgaaact ttaccgcggg tggcaagatc
    13321 ttctttggat aaaaatggac cgtcttttct tgcttcaacg atttgttttg ccacgttggt
    13381 tcccaaactt gggacagctc taaatggtgc gatcaacgta tcaccttcaa taacaaagtt
    13441 aacggcatcc gatttataca aatcaatcat tccaaatttg aatcctcgtt ccagcatttc
    13501 attggctaac tccagcactg tcagttgatt cttttctttt acggaagcat ctaatccttt
    13561 gtccgtgatt tctttcattg cttgtttcac agcatctttt cctttgcaca tggagactag
    13621 atcaaagtcg tctgcacgta cagaaaagta tgcacagtaa tacaaaatag ggaaataaac
    13681 cttgaaataa gcgacacgaa gagccatcaa tacgtaagct gcagcatggg ctttcggaaa
    13741 catatatttg atttttgaac aagaatcgat ataccagtcg ggcacattat tttctttcat
    13801 cgccgataga taggtttctc gcaattcatc agggatcttg ttccattgtc ctttacgaac
    13861 agtttccatg attttaaatg ccatcccgct atctaatcca gcatggatca aataaaccat
    13921 aatatcatca cgacagccga ttacttccgc cagtgtcgca tctcctcgtc gaatcaattc
    13981 ttccgcattt cctaaccata cgtctgtacc atgagatagt ccggaaattt gcagtagttc
    14041 tgcaaaagtt gtaggatgcg tttcttctag catccctcga acaaaacgag tgccgaattc
    14101 tggaatacct aatgtccctg ttttggaata aatttgttcc tgacttactc caagtacttc
    14161 aggaccggca aatatccgca tcacttccgg gtcatccgta gggatcgttt gtggatcgat
    14221 ccctgataaa tcttgaagca tccgaataac agttgggtca tcatgcccta gaatatctaa
    14281 tttcaataca ttatcgtgaa tcgagtgaaa atcaaaatga gtcgttttcc attccgaatt
    14341 ttgatcgtct gctggatatt ggatcggcgt aaaatcatag acatccatat aatcgggaat
    14401 aacgatgatc ccccctggat gctggccggt tgtccgtttg actccagtag cgccttttgc
    14461 taatctgtcg acttctgcac ttcgaaactg cagattattg tccctttcgt atccttttac
    14521 atagccatat gctgttttgt cggcgactgt accaatcgtt ccggcacggt aaacatagtc
    14581 ttcgccaaac aatactttcg tatagttatg ggcttctgct tgataatcac ctgagaagtt
    14641 caaatcaata tcgggcactt tgtctccatg gaatcctaaa aatgtctcaa atggaatatc
    14701 atgaccgtct ttgtttaagc gtgcaccaca ttttggacat tgtttttctg gcatgtcaaa
    14761 tccagaacca tacgtaccat cttcaaaaaa ctcggaatat tggcactctg gacaataata
    14821 atgaggggct aacggattaa cttcagtgat tcctgtcatc gtcgcaacaa agctagagcc
    14881 aacagagcca cgtgaaccta ctagatagcc atcttcatta cttttatgca ctagtttttg
    14941 ggcgatcaga taaataaccg agaagccatt accattaata gaattcaact ctttttttaa
    15001 tcgtttttga atgatctcag gtaacggatc tccgtacatt tgttttgctt ttgtgtaact
    15061 caattctgat atttcatctt ctgaacctgg aatcttcggt gtataaagtt catctttgac
    15121 tggaatcact tcttcgcaga tatctgcgat tttattcgta ttttctacta cgatctcttt
    15181 cgctgtttct tcccctaaaa agtgaaatgc agttagcatc tcatcggttg ttcggaaatg
    15241 gacatccggc aaactatgac gattcaacgg attcgctccg cccatagaat tgatcaaaat
    15301 ttttcggtaa atggcatctt cttcgttcag ataatgtaca ttacctgttg caacaacgat
    15361 tttatccaaa ctttttccga tttctaccaa attttggata atttcttcca aatcatgttc
    15421 attttttacc aattcttgct cgatcaaagg cgcatagacc gcttttggca taacttcaat
    15481 ataatcgtag aattttgctc ggtttcttgc ctcttctacc cctttttgca tcatggcttc
    15541 gaatatttct cccttatcac aagctgagcc gatcaataga ttttcacgca tctttttcaa
    15601 ttgggagcgt ggtattcgtg ggacgcgttc gaagtattcg acattcgaca ttgaaatcag
    15661 tttgaacaaa tctttcagtc ctgcctgatt ttttgctaaa atcgttacat ggaaaggtcg
    15721 agcccttttg taagaatctc cttctccgat atgctcattt agctgatcat gataaagcat
    15781 gttatgattg tccattgcct ctttcacaaa gatccatgct aagtgaccag ttgcttcagc
    15841 atcatagatt gctcggtgat gctgttccaa gctgacacca aattttttag ataacacacc
    15901 taatccgaaa cgtttgaatt gaggatacag atatctagct aattccaacg tatcaatgac
    15961 tggattggcc gcttcaggaa taccatacct tgcgtagcta gtattcaaga accccatatc
    16021 aaaagccgcg ttgtgggcta ccaaaattgt atcttttgaa aattcaagga acatccgcaa
    16081 tacttcttct tccgactttg atcctcgaac cattccatcc gtgataccag tcagatctac
    16141 cgttgttcgt gacaaaggat gtcctggatc gataaattca tcaaagcttt cgatcacatt
    16201 tcctttatac attttgactg cggctagctc gatgatcgtg tcatatactg cagacaatcc
    16261 tgtcgtttcc acgtcaaata cgacatatgt ggcttcactc aatgcttcat gtgcatcatt
    16321 ataagcaatt gggacaccat catctacgac attcgcttcc acgccgtata agattttcac
    16381 acctgctttt tttccagcac tatgtgcttc cggaaaggct tgcgcaccac cgtggtctgt
    16441 aatggcgata gctcggtgtc cccatttccc tgcttgtgcc acaagatcag agatgctgtt
    16501 tgttgcatcc atcgtactca tattgctatg aacatgaagc tctactcgtt tttccccttc
    16561 aggagcataa tctttgcgag gtgtatgttt gacttctatg atatcttgcg cattcatgac
    16621 caaatcacgg acaaacgtat cttcttgaat acttcctcgt acctttaacc agctaccgac
    16681 actgatcgca tcaaatactt gttcatcttt ttcgccgttc gagaattttt tgacgataaa
    16741 tgatgaagta taatctgtga ttttcaaagt caagatcttg cgttttgaac gtagttctct
    16801 gacttctttg tcgaatacgt acccctcaag tgtgactctt cgttcttctt caacgatact
    16861 gatcattggt gtcgttggtt catcggctgg aatatttcgt cctagctgga tgtggatggg
    16921 tccttcaaga gctggcgatt gctcttttct ttcctttttc ttttgttcgt ggacaactag
    16981 actttcagct gcttgcttca taaaggcttc tgcctgttct tgtttgcgtt cttcaaaaag
    17041 tttcaatact cgttccgctt gctgctcatc cacttctggt tcgatacgaa attttgggaa
    17101 tccgtaactg acgaacaact cttctactaa tggcaagtat tgttgcttca aataagaaat
    17161 agctcctgta ctgtcgattg gcaagatgac tttacgatct tttatcacag gtacctgcgt
    17221 tttcagtact tgctgtgcta gtggtgtatc gcattgctga ttttccaaag cttgggccca
    17281 gtaatcttgc aagagctgtt catcgaatgt ctggtcatct gctaagatat tcagtttgac
    17341 ttgtgcaata tctttaaaag cgatagtcag atgctgagtc aatgaccgat atagcataat
    17401 aggcaaaatt ttggaaaaat gcagcgtaaa ttcccataaa cgcgatttac gatggaccac
    17461 tacactatcc atttcaccag cagtaagcag tggatgattt ttttctgctt cctctagttg
    17521 gatttgttcc aacagttttt caaataaatc tcgtttttct gacaaagata tacgcccttt
    17581 ctgatgagaa acaatgcaat tgatcgcatc ttatcctctt ctttttcagt accttttagt
    17641 atactcgttt tttctgagtt ttacattaaa aaattggaac tgcgattcaa atcgcagttc
    17701 caattttaaa agcgaatcat cagattgtca aaaacagctg attcagctat ttttattttc
    17761 tgtgttttaa cgcttattat tcgccttggt tcaagaggat tggtaaagta ttcaccaatt
    17821 cttctttacg tacttcaacc atttcgccag ttttcttgat tttcacttct acgattccat
    17881 ccactgcttt tttaccgaca gtgattctga ttggacagcc aatcaaatca gaatccgcga
    17941 atttcactcc tgcgcgttca tttctgtcat caaccagtac ttcatagcca gcttttgtca
    18001 tcgcttcttc tagttcattc gtcaggtttg tttggtattc gtcttttaga ttcatttgga
    18061 cgatatgcaa atcaaatggc gcaattcctt ttggccagtt gattccttgt tcatctgaat
    18121 gttgttctac gattgctgat aataaacgac ttacaccgat accataacag cccataatca
    18181 caaatttttc tcgtccattt tcgtctagta cagttgctcc catagattca ctgtaacgag
    18241 tgccgatttt gaagatatgt ccgatttcga ttcctcgagt aaaggcaaga acaccattgt
    18301 tgtctggtga aggatctcct tcttgtacaa aacgaagatc ttcataagaa agtacagtga
    18361 aatcgcgttc tggattcaca tttttatagt ggaatcctgt ttcgttcgct ccagtaatgg
    18421 cattcgctaa atcttggaca taacggtctg catatacttt tacgtcatct gaaacaccga
    18481 ctggtccgac agaaccaaac tcagcaccta gatatttttg tgcttcttct tctgttgctt
    18541 cttctaaaaa gtcagcagat aaaaagtttt tcagttttac gtcattgact tcatggtctc
    18601 cgcgtaccaa aacaaggaca ggttgttcat ccgcaatgaa caatacagac ttgatgattt
    18661 tttgaggttc aacagaaaag aaatctgcta cttcgtcaat tgattttacg ttaggtgtag
    18721 ctactttctc caattcaagc tgtgtttcat gcgatttttt tgatacatag tagcttgtag
    18781 ccatctccaa gtttgcagca taatcacttt cagtcgaata acagatcgta tcctccccga
    18841 tttctgagat cgccatgaat tcttttgagt ctttcccgcc cattgcgcca ccgtctccga
    18901 taatcgagcg aaattctaag ccacaacgct tgaatacttc ggtgtaggct ttttcgtaat
    18961 ctttatatgt ctcgtccaaa ctttcttccg ttgcatggaa agaatacgca tctttcatga
    19021 taaactctct tccgcgaagc aaaccgaagc gaggtcgttt ttcgtcacga tatttaggtt
    19081 ggatttgata aagatttaaa ggtaatttct tatatgaatt gatttcatta cgcatcaaat
    19141 cagtaaaagt ttcttcgtga gttggtccaa ggatcatttt tcgatcattg cggtctttga
    19201 aacgatacaa gtttggtcca tatgtttcgt aacgcccgga ttcttcccaa agttctgctg
    19261 gcaagatggc aggcatcagc atttctaccg ctccgatttt ttcaaattct tcccgcataa
    19321 tgcgtttcag tttttccaat acacgatttg ctaatggcag atatgaataa atacccgctg
    19381 atacttggcg gatatatcca gctcttagca agatttgatg gcttaatact tctgcatcat
    19441 ttggaacttc gcgtaatgtt ggaatcaaca tttttgattg tctcattcta tatactcctt
    19501 tatttcctca aaatagttat cgtttgttat tataccctat ttgtcaactt tttctagaaa
    19561 aagaatcgtt gaatatcgtt ccatgtaact agcaccatca acaacattaa aaatccaaag
    19621 ccagctaatg tcaatattcc ttctttttct tgacttaatg gtttcccgcg aatcccttca
    19681 aaaatattca agactaattt cccgccatct aaggcaggaa tcggaagcag attgacgatt
    19741 ccgagattca ttgaaagaag cgccatcaaa ctaataacag ttgttacacc ttgatttgct
    19801 gcttctgaag acaattggta catcatgaca ggtccgccta atttatctaa actgaaacct
    19861 gtaaatagtg atcctaaggc tttgaaaatt tccaatgaac cgctgaaagc ttgacgcgtc
    19921 cctccaatga tcttatccat aaaacctgta ttcattgggg cttttatacc aagttggcca
    19981 actttttcgc catttgactc tacagatttc ggtgtcacgt cgacagattg cacctgacct
    20041 tctcgttcga tcttaaagtc taatgtttta tccggatttt tcgtaatgac tgttgtcaga
    20101 tcattccatg aatggatttc ttttccgtcc acactgacta cctcatcatt ttcttttaag
    20161 cctgcttctg ccgcagctcc attaggcatg atctctccta cgcgattagt gttcgtcact
    20221 tgaacaccac cttgcataaa tgccagaatg atgaataaca ggattgccaa aataaagtta
    20281 ttcatgggtc cagcaaagtt ggtcagcata cgctgccaca atttagccga ttggaactgt
    20341 acgtcttttg gcgcaattct gatttctgtt ccgtcgtttt caataatcgt cgcatcgtga
    20401 tcaacagggt aggttactac ttctgtttcg tctccattca cataaccagt gatagtcagc
    20461 tcatcttcta aatcataacg gctcaattcc atcgggatag catttgtcaa ttggattttc
    20521 ttacttaaat ttatcttctc tacgatgccg tcagaattta gtaaaagaga aagaggcatt
    20581 cctggtgcca tttctgtttc atcatcccca ttgcctgcca ttcgaacata accgccaata
    20641 ggtaataggc gaagcgtata tgttgtcccg tcttttgctt gatgcccata aattttcgga
    20701 cccataccga tagcaaattc gcgaacgagg attcctgatc gtttcgcaaa gaagaaatga
    20761 ccaaactcat gaacaatcac taatatacca aaaacgatga taaatgtcag aatcgttttc
    20821 atagcttttt tctcctttac ttgttttttg tgttttttat aaaacaacga taaaacacta
    20881 taattttacc atagtatacc aaagacacca atagtatcaa tataatgaaa aataaaaacc
    20941 tgcgtctttt atgaaattca caaagaaacg caggtatact tactaaataa tgccaaataa
    21001 atgcatgata ggaaatacaa acagcaaact gtcgaaacgg tctaagattc caccatggcc
    21061 tggtaagata tttcccgagt ctttcacatc ataatgacgt ttgattgcag attcgaccaa
    21121 atcaccaaat tgtccgactg ctgaaaaaat aatcgtcatc agaagcatga caaacatacc
    21181 atgattaaat agttcttttg ctgggtatag caataaataa agaaatgcga ctacaacagc
    21241 actaccaata ccaccgagcg caccttcaat cgttttgttg ggcgaaatat caggacttaa
    21301 cttatgctga ccataacgtc gaccaaccat atatgctccg atatcagttg cccaaacaat
    21361 aaatagacca aatatcaaaa cagccaagct ttctgttcgt gcattgacaa aattctggaa
    21421 acctattccc acatacaagc tgacaagcac aggaaaacct gcttcatcaa tcgtatacgt
    21481 atttttagaa ataactgatg ttcctaaaag aatcataact ataagataaa ataacataaa
    21541 tgtacttaca ttctctggaa gaaagaaaaa ccatctttct tttggcaaaa caagtaacac
    21601 tgcaccaacg gcggataaaa ctccttcaaa acttaagagt gtcaatcctt tcatccgaaa
    21661 cagctcgtaa actcctatta ctgctaagat tgctgctgta agctcaaccg ctatgcctcc
    21721 gatccatata atcggaataa acaagactag cgccacggct gctgtgacca cacgttgttt
    21781 catgactgat cccctttctc atcttctgtt ttttttacac cgccgaatcg acggtctcga
    21841 ttttgataag aggcgattgc ttcttcaaga tgcgcaccat caaaatcagg ccaaagcgct
    21901 tttgtaaaat acagctcact atatgcgatc tgccataaca gaaaattact aattcgctct
    21961 tctccacttg tacggatcat cagctcagga tcttgtaact ctttaggcaa gaaacctgtc
    22021 attaaatgct tagcaatcaa ttcatcatcg atttcttctg cgtgtatttc tccttttgct
    22081 acttcttcgg caatttcttg gacagcagtc acgatctctg ctctgcttcc ataattcaaa
    22141 gcaaaattca ataccattcc agtattgttt tttgtttgct caattgcacg tcttacagca
    22201 tcttgcgtat gttctggcaa tacgttttca taacccatga catgaacttt gacattttct
    22261 ttaatcaatt ctggtacgaa cgtatcaaag aagtcgacag gtagttgcat gagaaaactt
    22321 acttcatctt ttggtcgctt ccagttttca gtcgaaaagg cataaagcgt caacactttc
    22381 actccaagcc ttgatgcttt ttttgtgact ttctttactg tttccatgcc ttctttatgg
    22441 ccagcaactc gtggcaaacg acggttttgc gcccaacgac cattgccgtc catgatgatg
    22501 gcaatgtgct gaggaattgg tccatcggta ttaaattgaa attcgctttt ttcttgaata
    22561 tatttgtttt tttgcggaaa aaaacgtaac attctttttc ctcctatact taaaagattc
    22621 ccactatctt aagtattata gcaatatcta actgtttttc ctatgaaatc attgtttttt
    22681 tatttaaaaa ctgaataatt ttttcttttt ttaattttta aataaaagaa atataaataa
    22741 aaacagagga agcggaatga tccgcccctc tgtttttatg attttattaa acttccaata
    22801 attctttttc tttatcagca gcgatcttat ctacttcttt gatgctgtta tctgtcaatg
    22861 tttgtacgtc tttttctaat ccgcgcaaat catcttcggt aatatcacca tttttttgtt
    22921 gttttttata ttcatcgatc gcatcacgac ggatgttacg aatcgcgatt tttgcatttt
    22981 ctgcttcttt tttaacatct ttagctaatt cttttctgcg ttcttctgtc agttgcggga
    23041 taactaagcg aatgacattt ccatcattgg ttggactgat tccgatatca cttgccataa
    23101 tagctttttc aacatcttga agaatgctct tgtcaaaagg tgtgatcatt aaaacacgtg
    23161 cttctggaat ttggattgat gccatttggt tcaatggtgt tggcgcaccg tagtagttca
    23221 cagtaatacg gtccaaaagg ctagcgttcg cacggcctgc gcggatttgc ccaagttcac
    23281 gttgaagatt ttgtgctgct tttttcattt tttcttttgc ttctgtcata attgcatctg
    23341 ccatttttat ttccccctta cagttgttcc gatattttcg cctaagattg cacggcgaat
    23401 atttccgtgt tcatttaaat tgaagacaag cagtggaata tcattatcca tgcttagtga
    23461 acttgcagtt gaatccatga cttgtaatcc tttggcaata acttccagat gagtcagttc
    23521 ttcaaatttt actgcatttg catctacttt cggatcagca gaataaacac cgtctacatt
    23581 gtttttagcc atcaggataa catctgcgcc gatttctgcc gcgcgtaatg ctgctgttgt
    23641 gtctgtcgag aagtacgggt tgccggtacc gcctgcaaag ataacgatcc gtcctttttc
    23701 taaatgacgt tctgctttac ggcgaatata tggctctgcg atttgacgca tttcaatcga
    23761 agtttgcacg cgtgtcggta cacccacgtt ttccaacgta tcctgtaaag ctaaggcatt
    23821 cataactgta gctaacatac ccatgtaatc agcttgagca cgttccatgc ccatttgtgc
    23881 tccgatttgt ccgcgccaga tatttccgcc gcccacaacg attgccattt caacgcccag
    23941 ctcgtgaact tctttgattt cttggatgat ttctttgatt actggtggtt tgattccaaa
    24001 tcctgcgtcc ccagctaaag cttctccact caattttaag actacacgct gatatttcgg
    24061 tgtgaccatg ttcattcctc caatattttc tattgccatt ttaccatact tagatgattt
    24121 cgacactcta agactggttt ttcttgtttt gcttaaaaaa agggagcgca caaaccgtgc
    24181 gttcccgtct gttattcaac agaattattt tttcacttgg ctcataactt cttctacaaa
    24241 gttatcttcg cgtttttcga taccttcgcc aacttcgaaa cgtacgaatg ttttaacagt
    24301 tgcgcctttt gatgcaacgt atttttcaac agtcatatca ggatctttaa cgaatggttg
    24361 gtccaccaaa gcgatttctg ctttgaattt cttcaagcgt ccttctacca ttttttcaac
    24421 gatattcgct ggtttgcctt cgttcaatgc ttgttctgta agaacagttt tttcatgttc
    24481 taattcagct tctggaattt gtgattcgtt tacataacga ggattgatag ctgctacgtg
    24541 catagctacg tctcttgcaa ctgtttcgtc agttgttcca tctagaacag tcaatacggc
    24601 aatacgtccg cccatatgca agtatccacc aaatgcagca ttgtcgtctt tttctacaac
    24661 ttcaaagcga cggaagctga ttttttctcc gataacttga gttgcttcga tcaagtcaga
    24721 ttcaattgta cctttatctg ttttgatttt cattgcagct tccatgtctg ctggtttgtt
    24781 ttcagcaaca agttctgcaa tttcttttac taaatcttgg aacatttcgt ttttagaaac
    24841 aaagtctgtt tctgagttta cttcaacgat tgctgcagtg ttacctttca cagcaactga
    24901 agccaatcct tcagctgcaa tgcggtcatt tttcttcgca gcctttgcca ttcctttttc
    24961 acgtaaaaga tcaactgctt tttccatgtc gccttctact tcaactaatg cttttttcgc
    25021 atccatcatt cctacgccag tcatttcgcg tagttctttt accattttag ctgtaacgtc
    25081 tgccatgttt aactcctcct gtgtgttttt ctttttaaaa aaagctgctt caaaggagag
    25141 tgaggcttgt cgcccagcct ttgaaacagc tccatcattc aaaattattc tgctgaagaa
    25201 ttgtcgcctt caacaacatc tacgatttct tcgattgatg ttgctgtgtt ttcttctgtg
    25261 aaatcttctt caactacttg atcttcacct tggtttcctt cgatgaaagc atcagccatt
    25321 tttgaagtaa tcaatttaac ggcacgaatt gcatcgtcgt ttgaagggat tactacgtcg
    25381 atttcatctg gatcgcagtt tgtatcaacc atagctacga ttgggatatt taatttgtgt
    25441 gcttcttgaa cagcaatacg ttctttacgt gggtcaacga tgtacattac atctgggatt
    25501 cttggcatat cagcgatacc gcctaagaat ttttcaagac gttcacgttc tttgttcaaa
    25561 ccagcaactt cttttttagg tagaacttca aaagttccat cttcttccat tgcattgatt
    25621 tgtttcaaac ggctgatacg tttttggata gtatcccagt ttgtcaatgt tccgcctaac
    25681 caacgatggt taacgaagta ttgtcctgca cgagttgctt cttctttgat tgcttcttgt
    25741 gcttgttttt ttgtacctac gaataatgct acgccgcctt cttcagcaac gctcttcatg
    25801 taatcataag ctgcatctac taatttaact gttttttgca agtcaatgat gtagattccg
    25861 tttctttctg tgaagatata tttcttcatt tttggattcc agcgacgagt ttggtgacca
    25921 aagtgtacgc cggcttctag taattgtttc atagaaatta ctgccatgtt tgtttcctcc
    25981 gatttggttt tatttttcct cttcttatct tctaccttgc cggcaaactt tttcaagcac
    26041 ctaccgacaa tccaataaga atgtggattt gctgatgcat aaagcaccct cagctattat
    26101 acagacaaaa gatccgtatc gcaagagcaa atatactttt ctgctcaaca ttcaatagtt
    26161 tacctgaaag ataaaaagaa ttcaagaaaa acagtagatt tctctttgtt tctttattat
    26221 cctttttcca taaaataatt ttaggtataa aagacgaata ttattgggca atccttcttt
    26281 tttttgttat gatacatgtg aaatctttcg taaaggagaa agaaaatgcg caaagctcaa
    26341 agacatttgc tgattaaaca agtcattgaa gaattcccga tccgtacgca agaagaacta
    26401 ttaaacaaac ttgggcatta tggcgttacc gccacgcagg caacgatttc tcgagatatc
    26461 cgtgatctaa aaatcgtgaa ggcacctgat gaaaatggcg tttcccgctt cgtattgttt
    26521 cgcgaaaaag gaactcctga gactaggact gaggaagagg atcggctggt tcaaatgatt
    26581 gaagacattg tattgaaagt cgaacgagtc cactttttga cgatcgtcaa taccttgcca
    26641 gacaatgctc atttatttgc atctgtttta gatgagatca aacctccctc tattgtcagt
    26701 acaattgctg gattcgatac gatcatggtc atctcggaaa atgaggagga cgcaaaacga
    26761 gtagaagaat ttcttcacca tccaactgaa gtatcacttt tttaatcaga gcacgtattc
    26821 ctaatatgga atgcgtgttt ttttattttc aatattttcc ttattgaata aacctttaca
    26881 ctctttacaa aaatacatca ttttttattc aaatttttca acaccttgat tgaatcacaa
    26941 taatcatcat ttatattgga tgttaacaag agcagcttct tgctctttgt gtatttcaaa
    27001 aaagaaatga aaggaaatca tatgtataaa ttattaactt ctggaccatt gacgacttct
    27061 gaaacagtga aaaatgctat gctgatcgat cattgtactt gggatcaaga atacaaagaa
    27121 attacacaat ggataagaaa aaaattattg gatcttgctc aagtatcgga aggaatatac
    27181 acaacggtct taatgcaagg tagcagctcc ttcggggtag aatcggtatt aagttctgtg
    27241 atcaggccag aagatactgt actgatttgt tcaaacggcg catatagaca acgaatgatc
    27301 aacatgtgcg aatgtctgaa actgaactat accgtttatg aggtatcgga acatcaaatc
    27361 ctccaagctc atgtgattac acagctgatt gaagagaatc cgtctatctc cgcactagca
    27421 atggtccata gcgagactac ttccggtatt ttgaataatt tggaagagat tgctgctgtc
    27481 acacaagaaa gagatctttt gtttatagtg gacgcgatgt caagctttgg tgggataccg
    27541 atttgtgtag ccgaactagg gattgatttt ttgatttcca gctcaaacaa atgtattcaa
    27601 ggtgttccag gcttttcttt tgtacttgct aataaagcgg aactggaaaa acgaaaaaat
    27661 gttagccgaa gtttgtcact tgatctatat gaccagtacc gcgtgatgga aaacgagaat
    27721 ggcaaatggc gtttcacttc ccctacccat acggttttgg cttttcaaca agcgctgaag
    27781 gaactagagg cagaaggagg aatcgaaact cgatattcac gttatctcgc caataatcat
    27841 gtgctacgac aaaaaatgac agagatgggg ttccagcctt tcattcaaga atatcagggg
    27901 ccattcatca cctcttttct ttaccctaag gactggcgat ttgattttaa tcatttttat
    27961 cagtatttaa agcaaaatgg atttgtgatc tatcccggga aaatcagcga tcatgaatgt
    28021 tttcgtatcg gaaatattgg cgagatctat ccggaagata ttttggaact gacagcaatt
    28081 atcaaagact ataaggagaa cgttaatgat taaaactgta atttttgatt gggcaggaac
    28141 aactgtggac tttggctgta tggctcctgt ccacgctttt agaaatgctt ttctagaaaa
    28201 aggaatccaa ctgactgaca aagaaatccg ctaaccgatg ggaaaattaa aatgggatca
    28261 tatccaacag cttctttccc tcccttctgt caaaaaacaa tggatagcta tttatggaca
    28321 actacctcag gaaaaagagg tggaggaatt gtatcaacaa tttgaacatt atctgtttaa
    28381 agatttggtc aagcattcta ccttgaaacc cgacacattg gaaacgatcg aacggttgag
    28441 aaacgctggg atcaatattg gttccacgaa tggatttaat gctcctatga tgacaatcgt
    28501 agcagaaacc gcaaaaaaag aaaggtattc accagacttc atggcaactt ttgaagacgt
    28561 cgaagggtat ggtcgaccgt ttccttatat gatccataaa aacatgcagt acttcaaaaa
    28621 caagtcggtt gatgaagtaa tcaaagtcgg agatacggtc tcagatatcc aggaagggaa
    28681 aaatgccgga gtgttgacag taggtgtaat tgaaggtagt tcgctaatgg ggatttctta
    28741 tgatgagtac caaaaattag agatggggaa aaagatcgtt taaaacagtc tgttgctctt
    28801 agattccaac aagccggcgc tgatatttgt gtaaacaatt taaaagagtt aacagaatac
    28861 attttgtacg ctgacatgaa tccagaatga cttttataca aaataagagg ttgtgaaaaa
    28921 gctgtcctgt atcaagtaat aaaaacagcc aagcaaaaat agctcctcat atttttcgtt
    28981 tggttaggct tattaccgca gatgcaagct tttgaacacc gtttattcga aaaaaaaaag
    29041 ggagctgtga cacgactttt gtcacagtcc ccttatttgc aaacaaacag tgtttaagag
    29101 acaacctctc cactaaacgg tgttcattca gctgactctc ggtatttgct caaaaaacga
    29161 taaaatttga aaagctattt tctcttttgt ttctctaata ctcggatcag aacgctgatt
    29221 tcacattctc tttttcttac atcttatctg gtgcatgaag tcctagcaaa cgaaggtctt
    29281 ctttcaataa tacagtcact gcataaacta gagctaagcg tgcttctttt tgttcatcat
    29341 cagctaaaat cttcgtatga gcatagtatt tgttgaatgc ttgtgctaat ttgattgcat
    29401 gtttcgcaat cactgacggt tcgtattttt ccccagcaga caagaccgtt tcagggtatt
    29461 tttgaactaa tttaaccact tcccagctgg tatcgtcatt caatgcatag tctgctgttt
    29521 ctgaagggac aaatcctgct ttttccaaca ggctgactgc acgagcatgt gtatattgca
    29581 cataaggtcc agtttctcct tcaaatcgaa caacttcttc caggttaaag tcaaatgtat
    29641 tcagtcgatc gtttttcaaa tcgtggaaaa tgactgcccc aaccccaact tgtttagcta
    29701 cagcatcttt gttttccaaa tcagggtttt tctcgctgat ttgttcttta gcagattgga
    29761 ttgcttcatt taatacttct tctaataaga cgatctttcc tttacgagtt gataattttt
    29821 taccaccttg cgtgatcaaa ccaaatggaa tatggtgcat gtcgtcagac cagtcaaagc
    29881 ccatttcctt caagacagct ttcaactgtt taaaatgata gctttgttca ttccctacta
    29941 catatagaga ttgtttgaag tcgtaagtac gtttacggta caatgcagca gctaagtcac
    30001 gtgtaatgta tagtgtcgca ccgtctgact tcttaatcaa cgctggattc aggtcataag
    30061 cagataggtc aacgatctct gcacctttat cctcgttcag caaatgtttt tcttctaaaa
    30121 gtttgacgat ttcatccatc ttgtcattgt agaaagcttc accgttcaac gaatcgaaac
    30181 gtacttccag cagatcatag attttattga attctttcat cgattcatca cggaaccatt
    30241 gccataattg aatagcctct ttatcgcctt cttccagacg tttgaaccaa gcacgagctt
    30301 cttcatttaa ttctggttct gtttcagcta cttcatggaa ttggacgtat aaacgaagca
    30361 attcattgat tggttctgct ttaacagctt cttctgtacc ccattttttg taagcgacga
    30421 tcagttttcc aaactgtgtt ccccagtcac ctaaatggtt gatgcggatt ggctgataac
    30481 cgattttttc cataatgaaa ccgatcgaat taccgataac tgtcgaacgc aaatgtccca
    30541 ttgagattgg tttggcaata ttaggagaag acatgtcgat tggtactgtt ccttgattcc
    30601 cgatgttgct atccccataa tgttcttttt ctttcactac tgtttgcaaa acttttttgc
    30661 tgataagttc tttattcatg aaaaagttca agtaaggtcc gacaacttcg attttttcaa
    30721 agttggcaga atcgattttt tctgctaaat ctgcagcgat ttgctgtggc gcttttcggt
    30781 aaacttttgc taatgaaaag gctggaaatg caacatctcc gtgttcagct gattttggat
    30841 tttctagtaa ttgctcgact tgttccagtg ttaaatcatc tttgacagcg tcatagacag
    30901 cttttgcgac aagatcttta ttattcatct ttattcctcc taaagtgttc ccttgattgt
    30961 taaacaaaaa aaatctctag cgacggcata cttagtatgc catcactaga gacgagattt
    31021 cccgcggtac cactccaatt gtcctaaaaa aggaccagct ccatctgata tcggtagacg
    31081 accgtctgtt taaaacagaa ttctccaagt gcgcttcgtt gtatcttctt cattcggctt
    31141 ccaccacttc cgaattcgct agccaagaat gatacaagta ctctcttgtt caacaaatct
    31201 tagtcttatt aaactttctt gaattatatc agaaaaaaaa acgaaaggct agtcgtttca
    31261 taaaaaaaca atccagatgg cctaaaggag aagaaatacc acctggatag ctataaatca
    31321 tttcgtaaat acgaaagact taacaaatta atcagctacg ataatcgttc cttcttcaga
    31381 agctaaaaga ttctcgatat tttctaaaga agtgatgatt gccttagcgt ttggttttgc
    31441 ttcaacaaat gcaatagctg cttctacttt cggaagcatg cttcctggag cgaattgctt
    31501 ttcatcaatg tattgtttca tttctgaaac agtaacagtt tctaatttct tttgatctgg
    31561 tttttggtaa ttgacataca cattgtctac tcctgtcaat acgatcagca aatccgcatc
    31621 gatgatttcg gctaattttt ctgaagcaaa atctttatcg attacggctt ctcgtccttc
    31681 taatccagta gctgtttcta caacaggaat cccaccgcca ccaacagaga ctgtgataac
    31741 tccttgatcg accagcgttt cgatgacacg tgcttcttta atcgagatgg gtttagggga
    31801 agcgactact tttctccatc cacgtccagc atcttctttg aatgtcacag tggagtcagc
    31861 attcatttgt tctttggctt cttcttccgt gtagaatgga ccgactggtt tacttggatt
    31921 tttgaatgaa ggatcatttt catccacaat aacttgagtg actaaagaaa ccacatcttt
    31981 atcgatccct ttttccttca gtacttcgcc catggcgttt tgtaaccagt aaccgataga
    32041 tccttctgtc attgcaacac atgtatctaa aggcattgcc ggtgttttct cagaatcagc
    32101 agcttgctgc tgaatcaata ggttaccgac ttgcggacca tttccgtggg agatgatcaa
    32161 ttcatctcct tgttcgataa atttcactaa atactttgct gtttccatta acgcttcttg
    32221 ttgggctttt gcacttgcat ccgtagacaa gatggcattg ccccctaaag cgacaactac
    32281 ttttcgtttg gccatttcat tcgtcctttc ttcagttcta ccgaattttc tagataacaa
    32341 aacgagccag cgttttgtga ttttccctca atgctgactc gttttttcgt gagatgaatg
    32401 tggttatgct taaacgcgag ggataaacaa gttacctaat gttgctgcca tgatcgcttt
    32461 gattgagtgc atacgatttt ctgcttggtc aaattgacga gcatatttgc tgcggaagac
    32521 ttcatctgtt acttccattt cagtgatgcc aaagcgttct ttcatttgtt caccgtaaac
    32581 agtgtttgta tcatggaaag ctggcaagca gtgcaagaag atcaaacgat ctgtgttgtg
    32641 tgttttttct accatttcca tattgatttg gtaaggtttc aacaatttga tacgttcttc
    32701 gaatttatct tcttcaccca ttgataccca tacatcagaa taaaggacgt ctgctccgtc
    32761 tacaccttta tcaacatcgt cggtaatcat caattgtgca ccggattttt cagcaaagcc
    32821 ttctgccatt ttgacgattt cttcttcagg ttgtaattct tttggtgcaa cgatacgcat
    32881 attcgctcct aaaattgcac ctgtgactaa taaagagtta gccatattgt ttcgaccatc
    32941 tccgcagtaa gcaacagtga taccttctac tgttccaaag ttttcttgga tagttaaaaa
    33001 gtcagcgatc atttgtgttg gatgccattc atcagtcaag ccattccaaa ctggaacacc
    33061 agagaattct gctagttcct ctaccatttt ttgactgaat ccacggaatt cgattccgtc
    33121 aaacatacgt cctagtactt tagctgtatc ttctgtggat tcttttttac ctaattggat
    33181 atcatttgca cctagatatt caggatgtgc acctagatcg atcgctgcag tagtaaatgc
    33241 agaacgagta cgtgtagatg ttttttcaaa taaaagcgca atattttttc cttctagata
    33301 gtgatgtggg ataccacgtt ttttcaagtc tttcaagtgt tctgaaaaat cgatcaaata
    33361 ttgtaattct tcttttgtaa agtctttttc tgctaataag cttcttcctt ggaatactga
    33421 ttctttcata ttaaacattc tcctttagat tgtttttatt taagattaaa gaggaaatcc
    33481 cgacggcagc tgacagtaag actgccgtca ggaaacaaat tacttcaagt cttcacggac
    33541 aagtggttgg ctcatgcagc gtggaccacc acgacctcgt gacaattcac ttgaattgat
    33601 ttctaacact ttgatgccat agctacgaag caattcgttc gatacataat ttcggttata
    33661 agtgactaca acacctggag cgattgccaa tgtgtttgat ccatcattcc attgttctcg
    33721 aggtgcaacg atctcatctc cattaccagt tgggatcagt acaagatcat ctaatccaag
    33781 agcagctttc atcgtttcgt gtaagttatc tgaatgagta atcttgatat catcaccatc
    33841 tggttcgatt gtaaagacat ccatcttacc gtctttactt tgaattgctg gatggatagt
    33901 gaatttatcg tgatctacca ttgtaaatac ggtatccaaa tgcatcattg cacggttgtt
    33961 cggaattttg attgccaaca ctttttcaaa tcctgaattt tttgcgaata ggtggcgtgc
    34021 taatttttct aaagctttcg cattagttcg ttgagaaatc ccaacagcta ctactttatc
    34081 gcttaagatc aattcgtctc cgccttcgat atgatctgga ttttcacgat ccaaccatac
    34141 ttcgattcct ttatttgcaa atcttggatg atgtttcaaa atatattctg taaacatcga
    34201 ttcacgacga cgtgcttcaa aagtcatctt attgactgtc atcccatttc ccattgatgc
    34261 ggaagggtcg cgtgtaaagt acaagtttgg cattgggtcc atgtaaaatg gatagtcatc
    34321 gtctgctgac agatcatata gactgctaga acgtacttcg atatcttttg tacgaacccc
    34381 agccatgatt ttatctacca tttcttgcgt ttccattgat agaaggaact catgaagacc
    34441 ctctcttaca gcattcgatg caatatgaga ttcatccaac attttattca agaattgttc
    34501 tttgacattg cctgcgtcaa tcgcttctgc cgctaatttt tccaagtaaa gagtctcgac
    34561 gccttcattt tgcaatgtct tcgcaaagtt gtcatgttct tcttgagcaa tcggaagata
    34621 tggaatatca tcgaacaaca gacgatccat aatatctggt gtcaaatttt ccacttcttg
    34681 tccaggccgt ttcagtaaaa ctgtctttaa tttccctatt tcagagaaaa cgtgaatagg
    34741 tttatccatg ttgtgttgcc tccttctttt tgattacatg tataggatat cctccttttg
    34801 taatcacttt cataattttg tctcattcaa aatgagacat tcctcacatt aatattcgat
    34861 aattaaataa ttaaacgttt ttctattttt aagcgtaatt tat
//
LOCUS       DAYYGO010000016        34449 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000016 DAYYGO010000000
VERSION     DAYYGO010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.016"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            352..1041
                     /locus_tag="ACHA5T_000672"
     CDS             352..1041
                     /locus_tag="ACHA5T_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8886272.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="ACHA5T_000673"
     CDS             1028..2236
                     /locus_tag="ACHA5T_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFM8886273.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2530..3801
                     /gene="serS"
                     /locus_tag="ACHA5T_000674"
     CDS             2530..3801
                     /gene="serS"
                     /locus_tag="ACHA5T_000674"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFM8886274.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="ACHA5T_000675"
     CDS             complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="ACHA5T_000675"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFM8886275.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6339..7034)
                     /locus_tag="ACHA5T_000676"
     CDS             complement(6339..7034)
                     /locus_tag="ACHA5T_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFM8886276.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="ACHA5T_000677"
     CDS             complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="ACHA5T_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFM8886277.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8233..8424)
                     /locus_tag="ACHA5T_000678"
     CDS             complement(8233..8424)
                     /locus_tag="ACHA5T_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFM8886278.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8445..9335)
                     /locus_tag="ACHA5T_000679"
     CDS             complement(8445..9335)
                     /locus_tag="ACHA5T_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFM8886279.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9322..10089)
                     /locus_tag="ACHA5T_000680"
     CDS             complement(9322..10089)
                     /locus_tag="ACHA5T_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFM8886280.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="ACHA5T_000681"
     CDS             complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="ACHA5T_000681"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFM8886281.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="ACHA5T_000682"
     CDS             complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="ACHA5T_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFM8886282.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="ACHA5T_000683"
     CDS             complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="ACHA5T_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFM8886283.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14660..15043
                     /locus_tag="ACHA5T_000684"
     CDS             14660..15043
                     /locus_tag="ACHA5T_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886284.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15211..15969)
                     /gene="jag"
                     /locus_tag="ACHA5T_000685"
     CDS             complement(15211..15969)
                     /gene="jag"
                     /locus_tag="ACHA5T_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFM8886285.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15987..16805)
                     /locus_tag="ACHA5T_000686"
     CDS             complement(15987..16805)
                     /locus_tag="ACHA5T_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFM8886286.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="ACHA5T_000687"
     CDS             complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="ACHA5T_000687"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFM8886287.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="ACHA5T_000688"
     CDS             complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="ACHA5T_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFM8886288.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17934..19268
                     /gene="dnaA"
                     /locus_tag="ACHA5T_000689"
     CDS             17934..19268
                     /gene="dnaA"
                     /locus_tag="ACHA5T_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFM8886289.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDIKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19466..20596
                     /gene="dnaN"
                     /locus_tag="ACHA5T_000690"
     CDS             19466..20596
                     /gene="dnaN"
                     /locus_tag="ACHA5T_000690"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFM8886290.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            20825..21067
                     /gene="yaaA"
                     /locus_tag="ACHA5T_000691"
     CDS             20825..21067
                     /gene="yaaA"
                     /locus_tag="ACHA5T_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFM8886291.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21054..22178
                     /gene="recF"
                     /locus_tag="ACHA5T_000692"
     CDS             21054..22178
                     /gene="recF"
                     /locus_tag="ACHA5T_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFM8886292.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDSRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22175..24121
                     /gene="gyrB"
                     /locus_tag="ACHA5T_000693"
     CDS             22175..24121
                     /gene="gyrB"
                     /locus_tag="ACHA5T_000693"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFM8886293.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24141..26612
                     /gene="gyrA"
                     /locus_tag="ACHA5T_000694"
     CDS             24141..26612
                     /gene="gyrA"
                     /locus_tag="ACHA5T_000694"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFM8886294.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            26848..27147
                     /gene="rpsF"
                     /locus_tag="ACHA5T_000695"
     CDS             26848..27147
                     /gene="rpsF"
                     /locus_tag="ACHA5T_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFM8886295.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27197..27709
                     /gene="ssb"
                     /locus_tag="ACHA5T_000696"
     CDS             27197..27709
                     /gene="ssb"
                     /locus_tag="ACHA5T_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFM8886296.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27735..27971
                     /gene="rpsR"
                     /locus_tag="ACHA5T_000697"
     CDS             27735..27971
                     /gene="rpsR"
                     /locus_tag="ACHA5T_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFM8886297.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28114..30087
                     /locus_tag="ACHA5T_000698"
     CDS             28114..30087
                     /locus_tag="ACHA5T_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFM8886298.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30093..30545
                     /gene="rplI"
                     /locus_tag="ACHA5T_000699"
     CDS             30093..30545
                     /gene="rplI"
                     /locus_tag="ACHA5T_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFM8886299.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            30819..32186
                     /gene="dnaB"
                     /locus_tag="ACHA5T_000700"
     CDS             30819..32186
                     /gene="dnaB"
                     /locus_tag="ACHA5T_000700"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFM8886300.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            32966..34258
                     /locus_tag="ACHA5T_000701"
     CDS             32966..34258
                     /locus_tag="ACHA5T_000701"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFM8886301.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10504 a         6696 c         6824 g        10425 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2521 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2581 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2641 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2701 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2761 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2821 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2881 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2941 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3001 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3061 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3121 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3181 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3241 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3301 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3361 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3421 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3481 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3541 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3601 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3661 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3721 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3781 aatctgaaag ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga
     3841 aaaattttgt gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac
     3901 tggcttttga acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag
     3961 agaaaaaaat ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt
     4021 cagctgattg aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac
     4081 aaggacaaat tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag
     4141 gtggcaaggg aacactgttt attcaaaaag aggctgggac agaagcgttt aactccaaga
     4201 aataagaaga aattcacgaa aattgctttt caaatttttg tgaatttctt cttatttccg
     4261 aaggagttgc ttctacttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct
     4321 ttacttttgt cccacgctct ttaatgtttt taagattcaa ctgaatagtt tggtgcttct
     4381 tttgtgattt gtacatcatg agggtgtgat tcttttaatc cactgccgct catttggata
     4441 aattgcgctt catcacgtaa ttgctgtaga ttggctgccc caacataacc cattcctgaa
     4501 cgcaaaccgc cgatcatttg gaaaataata tctgcaacac ttcctttata agcaacccga
     4561 ccttcgattc cttctggtac taatttattt gcttcattga cactaccttg gaagtaacgg
     4621 tcttttgaac ctttttccat cgcgcctaat gagcccatac ctcggtatgt tttgaatcga
     4681 cgtccttgga agatttcgaa ttcacctggt gattcatctg tccctgccaa catacttcct
     4741 agcatcactg catgtccgcc ggcagctaat gctttgacga tgtcaccaga atatttgatc
     4801 cctccgtctg caataatcgc tttgccatat tgacgagcaa ctgatgctgc atcataaata
     4861 gctgtgagct gaggtacacc tacaccggct accacacgtg tcgtacaaat cgatcctggt
     4921 ccgataccaa cttttacgac atctacacct gcatcatata aagcttttgt cgcttcagcc
     4981 gtcgcaacgt ttcctgcaat caatgttgca tctgcaaaag ttgaacggat ttcttgaatt
     5041 tttcggatca cacctgcact atgtccatga gctgtatcga tgatgattgc atctgcccct
     5101 gcttctaata aagcattcgc acgctcaaat gtatcgcttg ttacaccaac ggctgctgct
     5161 acaagcaatc gaccatgttc atcttttgct gcatttggaa attcgatcac tttctcgatg
     5221 tctttgatcg tgatcaatcc gcttaaacga ccttcgttat ctacgattgg taatttttcg
     5281 attttatgtt tttgcaaaat cttttctgca tcttttagag aggtacctac tggggcagtc
     5341 actaagttgt cttttgtcat cacatcagca atcggcatgt gataatctgt tacaaaacgc
     5401 atgtcgcggt ttgtaattat ccctaccaat tttctgtttt caagtgtttc aacgattggc
     5461 acaccactga tacgatattt gctcatcaat tcttctgcgt ctgctacaag atgctgcggc
     5521 gttaagaaga acggatcaat gattacgcca ctttcagaac gtttgacttt tcttacttca
     5581 tccgcttgct ggctgatcgt catgttttta tggatcacac ctagtccgcc ttgtcttgcc
     5641 atagcaatgg ccatcttgct gtcggtaaca gtatccatac ttgcactgat gattggaata
     5701 ttcaacttaa tatttttagc taattgtacg ctcatatcca cttcgtttgg taacacgtga
     5761 ctttctgctg gtatcaataa gacatcatca aaagtaaatc cttttttcgt gaactttgtt
     5821 tcccagttgg acatttttag taccactcct ctaatttagt ttatctatta aaataattga
     5881 ttttttcatt taagtcaatg atctacttgg aatcaagtta gctttctcat agaataccaa
     5941 tataaagcgt ttacatctgt gtgtattaaa agagattgta aaaaagctgt cctgcatcaa
     6001 gtaataagaa cagccaatca aaaacagctt ctcatgtttt tgaattggtt gggtttatta
     6061 ccgcagatgc aaacttttga acaccgttta gtggagaggc tgtgaaaaag ctgcttagtt
     6121 tcgagtagta agaaaaattt tgtgaaaatg gctttccaca tttttgcata aattggactt
     6181 aataccgaga aactggcttt tgaacaccgt ttatcgtaaa aagggactgt gacaagactt
     6241 ttgtcacagt cccttactat tatttaacta tggataaaca aaatgaggtt ctttactacc
     6301 cctagatatc tagcaaaata gctgatcttt ttgctagttt atcttcttgg gacggataaa
     6361 cttccaataa tcccgtattt tttcttcaag tagtaacgga ttgctaaagc agttgccccg
     6421 ataatgataa ctactactgg atctaatact ggattgataa ctggtggcaa tagtgttgtc
     6481 gcagaaaaca gtacgaccca aagaagtgtc gttccaccta ggataagcaa tgtcttaccc
     6541 attcctggtt tcttgcttcg atctgctccg ggatattcat attgatagat gtatttgtac
     6601 atcatataaa agacccagcc accgattgca gaacctagaa cgagtgtaga aaagccataa
     6661 gctggcgttg ttccacgtga aaacaatccg gtaatcgcaa tcatgatact taatagacca
     6721 agcaacagca acgtattatc taaccacatc aataaagggg tggatgaatt ttttacttct
     6781 gctggcttat tgatgattgc ttccgttctt tcagagactg tcccaaacaa ttgacgggct
     6841 gttcttccgc ctttttgttc tgcaactaaa gtaggcagaa tgtcatgaag ggcttttact
     6901 ttttcttctt cagacaaatt cgctgcttct aaagattttt tcaaatcaaa aatgtactgt
     6961 tgatttcttt tggttaattt tgtttctaaa ctattgtttt cttttacgat tgctcgtaat
     7021 tgttctggtt ccattatcta atagtcctcc ttagccgttc cttttctcaa cggtcgtcac
     7081 aaaagacatt ctaccatatt ctacagctag tttgatagcc cttttacaca ttaaagcgga
     7141 ataacattac gtcgccgtct tgtacgacat aatcttttcc ttctaatcga acacgtccag
     7201 cttcttttgc tgcatgcatg tttccgtaat gatccaagtc ttcaaaagag acagtttctg
     7261 cacggataaa tccgcgttcg aaatctgaat ggatgattcc tgctgcttgt ggtgctttga
     7321 ttcctttttt gaatgtccaa gcacgtactt cttgttcccc agcagtaaag taagtagcta
     7381 agcctaacaa atcataagca gcacggatca actgatccaa acctgattct tcgattccta
     7441 aagcttctaa aaattccgct ttgtcttctt catctaattc tgcgatttct tcttctgcac
     7501 gagcactgac cacgatcact tctgcatttt cttctgcagc aaagttgcgc acttcttgta
     7561 cataatgatt cgcgtctgca tctgctacgt catcttctga gacattggct acataaagaa
     7621 ttggttttgt cgtcaataaa aatagtgatt taacgatttt ttgttcttct tcagtaaatt
     7681 cgatcgttcg ggcagatttt ccttcttcca gtacaggttt gattttatcc agtacagcta
     7741 gttctgccac tgcatcttta tctttggttt tagcaacttt tgctacacga gtatagcgtt
     7801 tgttgataga ttctaagtct gctaacacta gttccaagtt gatcgtatca atatcttcta
     7861 gtggatcaac acgtccttct acgtgagtaa tattatcatc atcaaaacag cgaacaacgt
     7921 gacaaatagc gtctacttgg cggatatggc ttaagaattg atttcccaaa ccttctcctt
     7981 tacttgcgcc ttttacgatt ccagcaatat ctgtaaattc aaaagtcgtc ggaacagttt
     8041 ttttcggatg tacaagttct gttaagcgtt gcaaacgcca gtcaggtact tctaccatcc
     8101 ctacgttggg gtcgatcgtc gcaaagggat agtttgctgc ttctgctcct gcttttgtga
     8161 tcgcattaaa caaagttgat tttcctacgt tgggcaaccc aacaattcca gcagtcaatg
     8221 ccataaattt attcactctt tctctgcatt tttttcaatt acttttttta atttcttttc
     8281 aaaatctcga cgggtcatca tgacgatatg tccgcaattc atacatctga ttttgatatc
     8341 tgcgcccatt cgaatgactt cccagcgatt cgtctggcaa gcgtgaggtt ttttcatttc
     8401 aacgatatcg tgcaagtcgt acataaaaat agcctccttt ttgtttattc ttcatcaaat
     8461 tgaatattca agatatcaag aattcgagtc aaatcttttt gagaaagata ttcaatctcg
     8521 atcttacctt tcccattttt ttcttggata gctacacttg taccaaattt atccatcaaa
     8581 cgatcttcgc tttctcggat ataatatggt ttttcctttg ctagccttgg gatttttttc
     8641 ttttcttttc catcattttg atttaactca gaaacaagct gttccagttg gcggacagtt
     8701 aaattttcag ttactgcacg attggctaat ttcaagatca atgttttatt ttttaaacct
     8761 aacagcgtac gggcttgtcc catagataaa cgctgatctt gtaccatttc cttgacaaga
     8821 tccggtaaag aaagcaagcg caaataattg gcgatataag gtctgctctt tcctaaacga
     8881 ctagctactt ctgcttgtgt tagtttcagg tttttcatca gcatctcata tgcttctgct
     8941 tcttctaacg gattcagatc ttctcgctgc aagttttcca gcactgctac ttgcatcatc
     9001 gcttcttcgt caaagtctct aacgatagca gggatcgttt ctttttcagc tagtttggac
     9061 gctcgaaaac gtcgctcccc tgcaataatc tcatatcctt ttactgcgga acggcgtacg
     9121 atgatgggtt gaaagacacc tgattgttca atagaattag ctagttcctg caaagatact
     9181 tcttcaaatg ttttacgggg ctgatacgga tttggtcgca gctcattcaa tggtagctgg
     9241 acaacggtat catcttttac atccaccgtc tctaagtttt ctaaatcttg aaacagtgca
     9301 tcgattcctc ggcctaatcc tttacttttc ttcacgactt accacttcct ttgccaatgc
     9361 ttggtagact tcagctcctc gagaacgtgg atcgtagtcg atgatcggtt ttccatgact
     9421 aggtgcttct gataaacgaa tatttcttgg aatgatcgtt tcgtaaactt tttcgcggaa
     9481 gtatttacgt acttcttcta cgacttcatt tcccagattt gttcgtgcat catacattgt
     9541 cagtaagact ccctcgatct ctaattctgg attaaagtgt ttttggacta aacgaaccgt
     9601 attcagtaat tggcttaatc cttccaatgc gtaatactca cactgaacag gtatcaaaat
     9661 cgaatcactt gctgtaaatg aattgattgt cagatggcca agagaaggcg ggcaatcgat
     9721 caaaatataa tcatactgac tgcttacttc tgctaaagat ccttttaatc ttgattctct
     9781 tgccatcatt gacgtcaatt cgatctctgc tcctgctaat tggagagtcg caggcacaat
     9841 actcaaattt tcgtgttcag tgatcaaagt tgcctcatca attggcaatt cattgactag
     9901 aacatcatag atgtctcttg tcacatcagg ttttctgatc ccgacaccac tggtagcatt
     9961 tccttgtgca tccatatcca ctagcaatac ttttttgcca aggctagcaa gacaagcacc
    10021 tagattcacc gtcgtcgttg ttttgccgac gccacctttt tggttcgcaa cagatattat
    10081 ttgtgccatc tcttctcctc ctatttgatt ggctgcttat taggcagtcc gggctttctt
    10141 gggtatttct ttggggttgg tttctttttt tgaataacga tgatatgccg ttgatcagag
    10201 acattcggca aggtaaccgc ctcttctttg aggaatttac cacctaataa agcaatcgct
    10261 tgttttgctt ctgtcaattc ctcttcactt tttgctgctt tcaatgcata aaaatagcct
    10321 tcttgtttag ccaagggcaa acacaattcc gctaacacat tcaaacgagc aaccgcacga
    10381 gcagtgacaa ggtcaaatga tgcacgaaag tgaggatttt gcccaaatgt ttctgcccgg
    10441 tcatgatata aggaagcatc gattcctaat aatcgggtta actcagataa gaaggttatt
    10501 cgtttattca gagaatcaac gatcgttatc tgcagttctg gaaaggcaat ttttaaaggt
    10561 atgctaggaa accctgcccc cgaaccaaca tcgcataaag ataaaggttg cgtaaagtct
    10621 acttcaaatg ctaatgtaag tgaatcgtaa aaatgcttca gatatacgtc tttttcatcc
    10681 gtaatagctg taaggttcat cttttcgttc cattcgacta atgtccgata ataaatagcg
    10741 aattgattca tttgatgatc ggtaagctct atcccttttt ctgccaagga ttggcgaaat
    10801 tcttctggta tcataaatat tcctttcatt gtgaaacagc aattgtttca cagattctct
    10861 ccctacttta acgcaaaatc tctagacatg caatccttcc atatggattc ttaaaaattt
    10921 gaaaagtaag aaaagccgat agactttttc tttctagtat gactcataaa atatacacag
    10981 aagagagaac tagctttgag caatttctgc aaaaactagt tctctcttct atttattatt
    11041 acgtattacg ctggttcact gactttcgca atcttccctt gttcgatata gaccattagg
    11101 atacttaggt ctgcagggtt tacgccactg attcggctag cttgggcaat tgtttctggt
    11161 tggattttct gcaatttttg ttttgcttct gttgctaatc cgttgattgc tgcgtagtcg
    11221 atgttttctg gaatcttttt cgcttccatg cgttttaatt tttctacttt ttccatggct
    11281 tttttgatgt agccttcata tttgatttgg atttctactt gttcgatttc ttttcgtgtc
    11341 aattcttcat tttctggaat aaaccgtaga agttctgcat aagtaatctc aggacgtctt
    11401 aaaaagtcgc ttgctagtac accatctttt aatggtgccg cattaatcgt tgctaaaaag
    11461 tcttggactt cttctgttgg tttgattcgt acattcatca aacgtttgat ttcttgttca
    11521 actgcttctt ttttcttcaa ataattcttg tattgatctt cttttactaa gcctacacgg
    11581 tgacccattt ctgttaatcg caaatctgca ttatcatgac gtaaaagcag acggtattca
    11641 gcacgtgaag tcagcaaacg ataaggttcg ttcgttcctt ttgttaccaa atcatcgatc
    11701 atgacaccga tgtaaccgtc acttcgtttg atcactaaag gttcttttcc ctgaactttc
    11761 aaagctgcat tgataccggc aatcagccct tgtccagccg cttcttcata accgcttgta
    11821 ccatttgttt gtccagctgt ataaagattt tcaacgactt tagtttctaa tgtcggacgg
    11881 agctggtgtg gctctactac gtcatattcg atagcatatc ctgtacgcat catttctact
    11941 ttttctaatc cagcaataga atgaagcatg ttgacttgaa catcctctgg taaagaagta
    12001 gataaacctt gaacatagac ttcttccgta tttaagcctt ctggttccaa aaataattga
    12061 tgacgtggtt tgtctgcaaa acgcacgatc ttatcttcga tggatggaca atagcgagcg
    12121 cctacccctt caacgatccc tgtaaacatt ggggcacgat gtaagttgtc tcgaataatt
    12181 tggtgagtcg tctcacctgt ataggtcagc cagcaagatt cctgatctag tttataggca
    12241 ctatctggtg tgctgaagct aaaatgattc ggctctttgt caccaggctg ctcttctgta
    12301 acagaatagt cgatcgtact tgattttact cgaggcggtg ttcctgtttt gaaccgttcg
    12361 atagcaaagc caagttcttt tagatgattc gctagtccaa tagaaggttg agagttattc
    12421 ggacctgatg aatatttcaa ttcaccgata atgatttctc cgcgaagtgc tgttccggct
    12481 gtaataacaa ctgctttggc ccggtagctc gctcctgtgg acgtcactac tccatgacaa
    12541 acaccatctt cgacgatcag ttcttcgacg attccttgtc ttagcgtcaa atttggttct
    12601 ttttcgatcg tatgtttcat ttctgttgaa taagcatgtt tgtctgcttg tgctcgcaat
    12661 gctcggacag ctgggccttt tcctgtgttc aacatacgca tttggatata ggttttatcg
    12721 atattcttac ccatttctcc gcctaacgca tcgatttctc gaacgacaac gcctttagct
    12781 ggtccgccta cagaaggatt acatggcatg aatgctacca tgtccaaatt tattgtcagt
    12841 aataatgttt ttgatcccat acgtgcagca gcaagtgcag cttctgaacc ggcgtgtcct
    12901 gcgccaacaa cgatcacgtc atacgcatct gcttcataat gttccaatcg tttgttcctc
    12961 cttttatttt cctaaacaaa attgactgaa taattgagtg attagctcat cttgtacact
    13021 gtcgccgaca acttctccta gatagtccca gcaacgcgtc atatctattt gaacgagatc
    13081 cacaggcatt cctgaatcaa tacctgcaat gacttcttca agtgagaggg aagctttttc
    13141 taacaacgcg atatggcggg tattagaaac ataagtagcg tctctttctc ctgtttctcc
    13201 gctgaagaat aaatcagaaa tagctgattc caaacgatct agtccgtcgt ttttagctac
    13261 ggaaattgaa aaaactggtt cattttcgat caatttcttc aatttttctt gttccagctg
    13321 tgctggcaaa tctgttttat ttagaagaat gatccgtttc aatccacttg tcgcttccaa
    13381 taattgttca tcttctgctg tcagtggttc gctttggttt agaacaagta agatcaagtc
    13441 tgcttctgct aatgcttttc ggcttctttc gaccccgatc cgttccacca catcttctgt
    13501 ttcccgaatt ccagctgtat cgattagctt gagcggtacc ccccgaacat tgacatactc
    13561 ttcgattaca tctcgagtcg ttcctgcaat atccgtaacg attgcttttt cttctcttaa
    13621 caaatggttt aacaaacttg atttgccgac atttggccgg ccgatgattg ccgtacttaa
    13681 cccttcccgc agaaccttcc cttgcttgga tgtagcaagt agcgcttgga ttctttgttg
    13741 gatcatctgc gctttttcaa gcaataactt cgtagtcaat tcttcgacat catcatattc
    13801 tggataatca atatttactt ctacttgtgc taatgtctct aaaatctctt gtctcaatga
    13861 gcggatcaaa gcagacagat tgccgtctaa ctgattaaga gctagtccca ttgctttgtc
    13921 tgtttttgcc cgaatcaaat ccatcacagc ttctgcctgt gaaaggtcaa cacgaccatt
    13981 caagaatgct cgttttgtga attcgcctgg ctcagctaat cttgcacctt ctcttaacaa
    14041 tagctgcaaa atttgattga ctacaacgat cccaccgtga caattgattt ctactacatc
    14101 ttcccgcgta aatgtctttg gtgctctcat cacggagacc atgacttcat cgacaagctg
    14161 attgctcttg ggatcaacga tatgtccata atggatcgta tgacttggta cttctgataa
    14221 tcgtttgttt ccagattgat aaactttatc tgctagttgc acagcttgat cgccacttaa
    14281 acgaacgata ctgattgcac cttctccagg tggggtagaa attgccgcaa tcgtatcaaa
    14341 ttctaaagta atttcagcca ttttttcact tcctttttca ttttgcgcac aaaaaaagtg
    14401 cccactccac cctttatgca caataaacgg tgaacttcgc actttgactg cttgttcttg
    14461 attaaattct ggtcatagat tatcacactt gctataagga atcaagtgtt taaaacaatt
    14521 agatgaagtt gagcctgttc tgcttccatt accttatatt taattgtcct tggatgtctc
    14581 tttgcgttct ttggtttatt cgaagagaag atactgtata aacaaggctt tatctttctt
    14641 aaaaaaaacg gtacactaaa tggtagcgct atctaaacta tacacttttg aaaggagaaa
    14701 atatgtgagc ccgttcagat ctgctgcatt ggaagaagaa agtgaccctc ggatcaacga
    14761 tgtcgtttta tctacaggga atatcaacca gaaatatatc gtccgcgata tcgtttttgt
    14821 tgtggaatgc ttagaagctg atttgtttga cccatctttt cccaaagatg accttttctt
    14881 aacaacgaag aggaaattaa agacgaaggt acttgaatat ggtgcgaatg cagtaatcaa
    14941 ctgtcatttt gagtatcagc aaaaaaaacg cgacggaaag tattttctgg aactttttgc
    15001 ttatggaaca gtgatccaat ttatccaaac aacgattggt taaatgaaac agacaaaaaa
    15061 agcggatcaa aactgaagac agtttcgatc cgcttttttc tatcaaagtt agtgactact
    15121 tcatcgtata ctctgataag aatgatttct agtaaaatat acttggtttg aaaaactttg
    15181 tcaacgtgat ttattcaaaa agtaatggaa tcaataatat tttttagcag gttctactac
    15241 taaataacga taaggttcct caccttccga atgtgttttt acatgttcat ctttgcttaa
    15301 tgcagcatgg atttgtttac gttcaaatgc aggcattggt tctaagaaaa caggctgccc
    15361 tgtccgtttt actttttcag ccgtgcgatc agctaaacgc tgaatgatct cttggcgctt
    15421 ttcacggtaa ttgcctacat tgactactac agacagctta tttcttgcta aacgatggat
    15481 gaagacttgt gccagatatt gcaatgcatt cagtgttttt ccatgtttcc caatcagtat
    15541 gccttgcttt tgtgtttcta aatggaaaac aaccatatta tttgattctc tttccatttt
    15601 gacaagagct ggagcatcaa gctgttttga aatttcagta agataaactg ccaattccgt
    15661 taaagctgct tcatcaccta gctcacttac ttcttctgtt ttttcttcaa gaatcgtttc
    15721 gttgactacc tcgatctcag ctgttggttc ttcttccaaa atttcttcta cagtctgttc
    15781 tactgtttca gaaacttttt cgctgatact tggctggatc gaaagccgtg cattctttcg
    15841 acccattcct aggaaacctt tttttccttc ttccagtatt tcgatttcga cttggtcttt
    15901 ggccaaaccc aaatcagcca gacctagttg gacagcttcg tctattgttg cagcttcata
    15961 aatcggcatt agcattacct cccctattat ttccgttttt tcttaggatt cattgctttc
    16021 ttaagagcac gttctctttc gcggattctc cgtgcttctt cttcacgctc ttttcgaatt
    16081 ttgaaagggt tgttgatcag cagcgtttgt acaacttgga aggcattgga aactacccag
    16141 tataatgaaa gaccgctggc taaattgaca cccatcacta aaatcattac aggcatcacg
    16201 aaattcatga tcttcattga tgcattcgat tcaatttgac tcatgctaga taaatacgta
    16261 cttgcaaaag taaagattgc tgctaaaatc ggcaaaataa atgttggatc cgcgttccct
    16321 aagttcaacc ataagaaatg cccttgtttc aactctggta cacgtgaaat agattgccat
    16381 aacgccatca agattggcat ttgtactaat aaaggaagac aaccagcata tggattgacg
    16441 ccattttctg catataaacg ctgctgctct tctcttaact tactttgtgt ttccggatct
    16501 cttgaactat attgttgctg cagtgctttt aatttcggtt gtaattcttg cgttttacgc
    16561 atgctctttg tttggaaatg catcaacggc aataggatca cgcggatgat cagtgtaaat
    16621 aaaatgatcc ctatccctgc attaccaaat gacaacgcct tgatcgcctc agcaaagtaa
    16681 taaacaatat aacgatccca aattcctgta ctctgtgcgc tgacttcccc agttccgcaa
    16741 ccggataaga cgatgaccaa actaacgata ccggccatca aaagtaaacg tttgtatttc
    16801 ttcacttact cttttcctct ctgtcgatta tttttgcaag ttttaagacg tggactaaat
    16861 tggagcgaac ctcatcataa gtcaacccct tcacacttgg tctagcaatc acaataaaat
    16921 ccaactccgg atcaatttct tcttttatag aataaactgc tgctcggatt tttcgtttga
    16981 cttcattgcg gtttaccgcg ttgccgacct tttttcctac agataaacca actcgaaaat
    17041 gaggctgtcc cggtttttcc aaacggtaaa cgacgaattt gcggttagca aaagaatctc
    17101 ccgtatggaa cacggcttga aactctcgtt ctttttttac tcgatacgct tttctcatta
    17161 gttttctttc cttttctatc tctcacttac gctcataatc ataccataat tacgattgtc
    17221 ctttcatcat ttccctaaaa aagagtataa aaaaaaccac tgaaacccca gtggcctaag
    17281 ctgaaagaac ttttcttcct ttgcgacgac ggctagctaa cacacgacgt ccatttttag
    17341 tactcatacg tttacggaat ccgtgaactt tttgacgttt acgtttattt ggttggtatg
    17401 ttcttttcat atattccacc tccaaaatgt tcttatctat tttataaaca cagacatact
    17461 ttgatagtat aacgacaaag tagtgatttt gtcaatataa aatagaagat cgattgttaa
    17521 aacttatcca caagattatt tttttctatg ggagtcctat gtggaaaaag ttatgcacag
    17581 ggattatcca cagataataa atggctggtt ttacacagaa aacacacgtg taaaaaaagt
    17641 aatccacaat caggtgattt cctgtgtatt ccgtctccta actattggta tatctatatt
    17701 ttttatccac agatgcaaga ggaaatctat tgatttttac gagttttcca cctgtgcatt
    17761 agtggtgtgg aaaacttttt tttgacaact gtgtttttct cttttttctt tttgtcgatt
    17821 gtgtggataa ttgtaattaa aaaatttttt tcttcggttt ctgtggaaaa ctattgaaaa
    17881 aaatgctaaa ttagtactag ttattcactt tttaatgaag gaggaggcct tatatggtat
    17941 ccctcgatgc tttatggaat gaattaaaag caacatacca aaaggattta tcgcctgcta
    18001 gctataatac atggatcgaa acagcgaacc ctcgaaccct tgatcaaaac cagctcgttg
    18061 ttgaggttcc tagcaagatt cataaagaat attgggaaaa aaacctggcg acaaaaatcg
    18121 ttgaagtcgg ttatatgtta tcaggaaatg agattatccc tcgctttatt actggcgaag
    18181 aagcagagca agaagaagtt atagaagaaa aaaatccaaa agttgtggca cctagtccgc
    18241 taaaaaaagc catgctgaac cctaaatata cctttgatac gtttgtcatt ggtaaaggaa
    18301 accagatggc tcatgcagca gcgctcgttg tagcagaaga tccaggttct atttataacc
    18361 cgctcttctt ttatggaggc gtgggacttg gtaagactca cttgatgcat gcaattggtc
    18421 atcaaatgct gcaaagtcag cctaatgcca aagtaaaata tgtaagcagt gaaacttttg
    18481 ctaatgattt catcaattcg atccaaaaca aaacagcaga agagttccgt caagaatatc
    18541 gaaatgttga tttactgcta gtggatgaca ttcaattttt tgcagaaaaa gaagctacgc
    18601 aagaagagtt tttccatact ttcaatgcct tgtacaatga agggaaacaa atcgtactaa
    18661 ccagtgatcg gttgccaaac gagattccga aactgcaaga acgcctcgtt tctcgctttg
    18721 catgggggct gtctgttgat attacaccgc cggatcttga aacaagaaca gcgattttac
    18781 gtaaaaaagc ggcggctgaa cgtctagaga tcccagatga cacactaagc tacatcgcag
    18841 gacaaattga ttctaacatt cgtgaattag aaggtgcgct tgttcgagtg caagcttttg
    18901 ctgcgatgaa tagtgaagat atttcgacta gtctagctgc agacgcactg aaaacgttaa
    18961 aatcaggaaa aggacatcct caattgtcaa tcctgcagat ccaagaagaa gtggcaaagt
    19021 attatcacat ccaattgaaa gatatcaaag gaaagaaacg cgtcaaatct attgttgttc
    19081 ctagacaaat cgctatgtat ttggcacgtg agctcacaga taattcttta ccgaaaatcg
    19141 gagctgaatt cggtggcaag gatcatacaa cggttatcca tgctcatgaa aaaatccagc
    19201 agctgatgga ttctagtgtc agtatgcaaa atgaagtatc agaaatcaaa aactttttac
    19261 taaattaatt tgtcgattct atcaactgaa aatagcatgt ggataaaaga aaaaaacgtt
    19321 caaaagttat gcacactttt tccacagctg ttttttcgtt tctctcttac gttttttgag
    19381 ttttccacag aatcaacatg ccctattact tttattattc tttttaacta ataataaata
    19441 aacaaacatt caaaggagat acatcatgaa agttacttta aaccgagcta gctttatgca
    19501 ggaattgcaa actgttcaac gagctatttc aagcaaaacc acgatcccta ttttgacagg
    19561 tgtaaaaatc acactgacac aagaaggttt gactttgacg gggagcaacg ctgatatatc
    19621 aattgaaact tttttgtctg ttgaaaacga aaaagcaaat atgcaaatcg aatctactgg
    19681 ttccattgtt ttacaagcac gtttctttag cgaaatcatt cggagacttc ctgaagaaac
    19741 atttacttta gaagttttag aaaataaaca agtagcgatt acttctggaa aagcgaattt
    19801 tatcgtaaat ggattagatg cagataacta tcctcatctt cctgttgtcg aaagccataa
    19861 ccagatgaaa ttacctgtac acgtattgac taaactaatc aacgaaacag tttttgctgt
    19921 ctcacaacat gagagtcgtc caatcttgac aggtgtgcat ttcattttat ctgataattc
    19981 tttattggct gtagctactg attctcaccg tctaagtcaa cgcgtgattc cagtagaaca
    20041 agcggctgat cattttgata ttgttattcc tggaaaaagt ttgatcgaat tatctcgctc
    20101 attaacaaat gaagaagaaa tcgtcgaaat cagcattatg gaaaaccaag tgttgttcaa
    20161 aacagaaacg atgtatttct attcccgttt gctagaagga aactatcctg ataccaatcg
    20221 tttgattcct tcaagtttta atacagaggt tgaattttct gttcctagct ttttagcggc
    20281 gatcgaacgg gcttctttac tttctcatga aggccgtaac aacatcgttc gtttatctat
    20341 tcgtccagat gcggttgttt tatacggaaa ttcgccagaa atcgggaaag tagaagaaag
    20401 cttgagttat acagcaagca gtggcgatcc attagatatt tctttcaatc cggattatat
    20461 gaaagcagcg ttgcgtgcat ttggagatat gagcatcaaa gtgaaattta tctctgctat
    20521 tcgtccattt acattggaac caacggaaga tggcgttcaa tttattcagc tgattactcc
    20581 tgtacgtaca aattaacaat gattttcatt gttcttgaaa atttaattga aaagaaatag
    20641 aaaaaataaa attttaaaca gaaggcccta aaatctcttt taaggctttc tgtttatttt
    20701 tgtttaaatc acctttttta gattaaatcg tcttatatgg aaaaatatat ggataaattt
    20761 gttttattgc gaaaaaaaag gtataataga gtatactttt aataatgaaa atgagggatg
    20821 ggcattgaaa caacaagtca ttctaaatac ggaattcatg acgttgggcc aaatgttgaa
    20881 agaagtcaca gttatcggta gtggcggaca agcaaaatgg tatttggcag aaaacactgt
    20941 gttagtcgat ggcgaaccag aaaatcgtcg tggacgcaaa ctttatcccg gcatgatggt
    21001 tgaagttcca gaagtaggaa ctttttttat ggtaaaaaaa ggaaacgacg ataatgaggc
    21061 tgaataagct gtatttaaaa aattatcgaa attacgaaga gctgaacatc gaattttcca
    21121 agaacctcgt catcttttta ggagaaaatg cgcaaggaaa aacgaatctt ttggaaagta
    21181 tctacgtatt agctatgacg cgaagtcatc ggacaagtag tgagcaggaa ttgattggct
    21241 ggactgacga tcaggcgatg attcaaggag agatcacaaa aggttcatcc actcttccat
    21301 tagaaatctt attatctaaa aaaggccgaa aaaccaagat caaccatatc gaacaaaaaa
    21361 agttaagtac ttacgtggga caaatgaatg tcattttatt tgcaccagaa gatctttctt
    21421 tagtaaaagg cagcccgcag atcaggcgga agtttttaga tatggaatta ggccagatca
    21481 atccaatcta tctttatgat cttgtccaat accaagcaat tttgaaacaa agaaaccagt
    21541 atttaaagca attgaatgaa aaaaaacaaa ctgatttgct ttatttggat attttgtccg
    21601 aacaactagc agcgtttggc agcaaagtat tgaaagcaag agcacagttc gtcaagcgat
    21661 tggagttttg ggctaattct ttgcatcagc aaatcaccca tcaaaaagag cagctggaaa
    21721 tcgagtatct caccgcagtc gattctttag aaacacatac gcaagagcaa atccaagaac
    21781 aatttctcgc acttttgaac caaaataaaa agaaagacct ttttcgggga acaacgacag
    21841 ttggtcctca tcgtgacgat ctttcctttt ttattaatca gaaaaatgtg cagacttatg
    21901 gatcgcaagg acagcaaaga acaactgcac taagtgtcaa attagcagaa atcgatttga
    21961 ttaaagaaga gacgggggaa tatccaatcc ttttgttaga tgatgtgatg agcgagctgg
    22021 atgatagtcg tcaactccat ctgctagaaa caatcgaagg aaaagttcaa acgttcttaa
    22081 caacaacgac acttgaccat gtaaaaaata aaatgactgt tgaacctgag attttttatg
    22141 ttcaacaagg gaaactaaaa ggagaagaaa ctgaatgaca gaagaaagaa gtttagtaga
    22201 acgcgctaaa gagtatgatg ccagtcagat tcaagtactt gaaggacttg aagccgttcg
    22261 taaacgacct ggcatgtata tcggttcgac tagttctgag ggtctgcatc accttgtttg
    22321 ggaaattgtc gataattcga tcgatgaagt attagcaggt tttgcaacga aaatccatgt
    22381 gattattgaa aaagacaaca gtatcaccgt tattgacgac ggtcggggga tacctgtaga
    22441 tatccaggca aaaactggac gtcctgcagt tgaaacggtc tttactgttt tacatgctgg
    22501 aggaaaattc ggcggtggtg ggtacaaagt ttctggtgga cttcacggag taggatcttc
    22561 tgttgttaac gcgctttcta cacagcttga tgtcaaagta tacaaagaag gaaatgtgta
    22621 ctatcaagaa tatcgtcgtg gagctgtagt ggacgactta aagattatcg aacaaacgga
    22681 tcgccatgga acaacggttc actttacgcc agaccctgaa atttttacag agacaactga
    22741 gtttgatttt tcaaaattgg ctacccggat ccgtgaactt gctttcttga accgcgggat
    22801 gcgtatatcg attgaagaca aacgagaaga agaaccagtc atcaatgaat accattatga
    22861 cggcgggatt aaaagttatg tcgaatactt gaatgcaaac aaaaccgtga tttttccaga
    22921 acctgtatat ttggaaggtg aacagcaaga tatcgcagtt gaagtctcta tgcaatatac
    22981 agacggctac cattccaata tcatgagttt tgccaacaat atccatacgt atgaaggcgg
    23041 aacacatgaa tcaggattca agactgcttt aactcgtgtg ataaatgatt atgcccgaaa
    23101 acaaaagctg atgaaagaaa atgacgacaa cttgaccggt gaagacgtgc gagaaggatt
    23161 gacagctgtt atttctatca agcatcctga ccctcaattc gaaggacaaa caaaaacaaa
    23221 attgggcaat tccgaagtac ggacagtcac tgatcggtta tttagcgaac atttcatgaa
    23281 attcttgctg gaaaacccaa gtgtgggccg tcaaattgtt gaaaaaggat tactagcttc
    23341 cagagctcgt ctagcagcga aacgtgcgcg tgaagtaacg agacgtaaag gtgctttaga
    23401 gattagtaat ctgccaggaa aattagcgga ctgctctagc aacgatcctg aaaaatgcga
    23461 attgttcatc gtcgaaggag attcggccgg cggatcagct aagcaaggtc gtaatcgaga
    23521 gtttcaagcc atcttgccta ttcgtgggaa aatcttgaat gttgaaaaag caagcatgga
    23581 caagatcttg gcaaatgaag agatccggtc tttatttact gctatgggta cagggtttgg
    23641 agaagatttt gatgtatcga aagctcgcta ccacaaatta gttatcatga ctgatgccga
    23701 tgtcgacggc gcgcatatcc gtacattgct gctgactctt ttttatcgtt acatgcgtcc
    23761 tattgttgaa gctggatacg tctatattgc tcagccacct ttatacgggg taaaacaagg
    23821 gaagaatatt acgtatgtac aaccaggaaa aaatgcagaa gaagagttga aacaagtcgt
    23881 tgcttcactt cctgcctcac caaaaccaag cgttcagcgt tataaaggac ttggagaaat
    23941 ggatgatcat caactatggg aaacaactat ggacccaagc aatcggatga tggcccgcgt
    24001 cagtgtggac gatgctattg cagcagatca gatatttgaa atgttgatgg gggatcgagt
    24061 agaaccccgt cgtgcattca tcgaggaaaa tgcacactat gtgaagaact tagatattta
    24121 atcatgaagg aggaaaaaga atgagtgaag aaatcagaga aaacatccat gatgtcaatc
    24181 taaccagcga aatgaaagac tccttcatcg attatgcgat gagtgttatt gtcgcacgtg
    24241 cacttcctga tgtcagagat ggtctgaaac ccgttcatcg ccgtatttta tacggcatga
    24301 acgaattagg tgtaacaccg gataaagccc ataaaaaatc tgccagaatc gtcggagatg
    24361 tcatgggtaa ataccatcct catggagact atgcgatcta tgagtcaatg gtacggatgg
    24421 cacagccatt cagctaccgt tatatgctag tggatggtca tggaaacttt ggttctgttg
    24481 acggtgacgg tgccgccgct atgcgttata ccgaagcacg gatgagcaaa atcgctacag
    24541 aaatgctgcg tgatatcaat aagaacactg ttgatttcca aagcaactat gatgatacag
    24601 aaaaagaacc agttgtacta cctgctcgct tcccgaatct tttagtgaac ggaacaacgg
    24661 ggatcgccgt aggtatggcc acgaatatcc cacctcataa cttgtctgag gtagtagcgg
    24721 ctatcgattt gctgatggat aatcctgatg tgacgaccaa tgaattgatg gaagtattac
    24781 ctggaccaga ttttccaaca ggtggtttgg ttatgggaaa atcaggaatc cgaagagcgt
    24841 atgaaacagg taagggttct attaccgtac gtgcaaaagt tgagattact gagatgccaa
    24901 atggtaaaga acggatcatc gtgacagaat tgccttatat ggtcaataaa gcaaaattga
    24961 tcgaacggat ttctgaactg cacagagaca agcgtatcga agggatcaca gatttgcggg
    25021 acgaatcttc ccgtgaaggt atgcggatcg tcattgacat ccgtcgtgac gccagcgcat
    25081 ctgttatctt gaataactta tataaattga catccttgca aaattcattt ggattcaata
    25141 tgttggcgat cgaaaaaggt gtgccgaaag ttttgagctt gaaacaaatt cttgaaaact
    25201 atatcgaaca tcagcgcgaa gtcatcacaa gacggacagt ctttgaaaaa gaaaaagcag
    25261 aagcaagggc acacatttta gaaggtttgc gtattgcttt ggatcatatc gatgaaatca
    25321 ttgcgattat ccgcgggtct aaatcagatg acgaagcaaa agcgacgatg atcgaacggt
    25381 tcgatttatc cgatcgccag tctcaagcta ttttagatat gcgtttacgc cgattgaccg
    25441 gtttggaacg ggaaaaaatc gaaaatgaat accaagaact cttaaaactg attgaagatt
    25501 taactgacat tctggcaaga ccagaacgtg ttacagaaat catcaagaat gaattagcag
    25561 acttgaatca acgatttggt gataaacgac gcacagaatt actagtcggt gaggtcttaa
    25621 gtctggaaga tgaagattta atcgaagaag aagaaatcgt tattacattg acaaacaatg
    25681 gttacatcaa acgtttggca aataatgaat tcagagctca acgaaggggt ggacgtggtg
    25741 tccaaggaat gggtatccac aatgacgact tcgtcaaaaa tctggtttct tgttctaccc
    25801 atgatacatt gttattcttt acgaataacg gaaaagtgta tcgggcaaaa ggatacgaaa
    25861 ttccagaata cgggcgcaca gccaaaggaa taccaattat caatttacta ggtatcgatt
    25921 cgagtgaaag catccaggca atcattgcgg tgacgggaga agcagaagaa ggccactatc
    25981 tcttctttac gacgcgtcaa ggtacagtaa aacgtacttc tgtcactgct tttgctaaca
    26041 tccgaagtaa tggtctgatc gcaattggtt tgaaagaaga cgatgagctc gtcaatgtgc
    26101 tactgacaga tggtcaatct aatatcatta ttggaacaca taacggctac tctgtcactt
    26161 tcaaagaaga agcagtccgc gatatgggac gtacagcttc cggggttaga ggaatacgcc
    26221 tgcgtgagga agattatgtc gttggggcag ccttaatgaa ggaagatcaa gaagtattag
    26281 tgatcacaga aaaaggttac ggaaaacgga caaaagtatc ggaatatcca gtgaaaggcc
    26341 gcggaggtaa agggatcaaa acggcgaaca tcactgaaaa aaatggtcca ctagctggat
    26401 tgaccactgt ttctggagat gaagacatca tgctgattac agataaaggt gtcattatcc
    26461 gtttcaacgt atcgactgtt tctcaaactg gacgttcaac attaggcgta cgtttgatga
    26521 agatggaagc tgatacgaaa gttgtcacga tggcagcagt cgaaccagaa gctgcagaac
    26581 aaacagttga agaacaagga tcagaagaat agccatcaag actaattgtg taagctagca
    26641 agaatgaccg attaaaaaat ctggttattc ctgctggctt ttttgattag ttgaatttct
    26701 cttaaaatat tcgaaactgt tcattctgta ttgcttttga ccaaaataga gtgtataatc
    26761 agagcttgtg agtagtctaa ttaattagat aactctcctt gctcttagga acctaagagc
    26821 cgaaagtcca cagggaggtg aatatcaatg gaaaacacga aatatgaaat catgtacatt
    26881 attcgtccaa acattgatga agaagcaaaa actgctttaa tcgaacgttt cgatgcaatc
    26941 ttgaaagata acggagcaga agttatcgaa tcaaaagatt gggaaaaacg ccgtttcgct
    27001 tatgaaatga acggttaccg tgaaggtatc tatcacatcg ttaaagtatc ttctccatca
    27061 tcagcaaatg ctgtcaatga atttgaccgt cttgctaaaa tcaacgacga tatcattcgt
    27121 cacatgattg ttaaagaaga agcttaattg ttgtttcatg tgaaacaata gaaaacgatg
    27181 agaggagatg actattttga ttaataatgt tgtattagtt ggacgtctga cgaaagatcc
    27241 agatttgcgt tacacagcaa gtggtacggc agtcgcaaca tttactttgg ctgtgaatcg
    27301 taattttaca aaccaaagcg gcaatcgcga ggctgatttt atcaactgtg tgatttggcg
    27361 caaatcagca gaaactttag caaactatgc tcgtaaagga acacttttag gtgttactgg
    27421 acgtatccaa actcgttctt atgataatca gcaaggacaa cgtgtatacg tgacagaagt
    27481 agtagctgac aacttccaat tgctggaaag tcgttctgca tctgaaaatc gccaacaaag
    27541 cggcggattc caaagttctg gtcaatcagc tggcggattt ggcggaaaca ataactctaa
    27601 tcaaacatct caatcatcca acggtatgcc tgatttcgac cgtgatactt ctgacccatt
    27661 cggctcaagt tcaacgatcg acatttcaga cgatgattta ccattctaaa cgaatttgat
    27721 aggagggaaa tagaatggca caacaaagaa gaggcggacg taaacgtcgt aaagtagact
    27781 acattgctgc taaccatatt gaatatatcg attataaaga tatcgaatta ctaaaaagat
    27841 ttatttcaga acgcggtaaa atcttgccac gtcgtgtaac tggtacaggc gctaaaaacc
    27901 aacgtaaaat tactgttgca atcaaacgcg ctcgtatcat gggcttgcta cctttcgtta
    27961 gcgaagacta ataaatccta ggaattgaat gactgatcct tttgatcagc tattcaattc
    28021 ctttttttct gtaaaaataa cctctattcg tttagtcgct cgaatgacga aagttatggt
    28081 aaaatcaaat tagtcatata tgggaggaaa attatggaaa ataaaaacaa tcatttatcc
    28141 ccgaaacctc tcttccttat cctgtttata ttaatcgaag tattggtagt cttaatttta
    28201 ccatttcgtt ttgttagcgc ttcggtcatt ctattgatga atttatgggg tgttcgctat
    28261 tggttgaaaa tgaaacgttg gcttatttta aataaacgtg aaaaaatcca gcaggcttcg
    28321 actgtagctg aaaccaatct agcttatgtc tcagaagtca tgcctgtagg agtgattgct
    28381 tatcagccat caagccaaaa aatcgaatgg ctcaatccat tagcattgaa aatcaaagat
    28441 caaagtggat taacagagga agaattgcta gatacttttt ttactgcaaa agaaaaaaag
    28501 aaaaaccaag tctatctagc agataaaaca tttcagtttg atatggatac agtaaagggt
    28561 gttgtttatt ttaatgatat tacccaaaac cagttgttaa aactccatca gacgaacagt
    28621 cagccggtga ttggtatttt atctattgat aactatgcag atgccattga taaattggat
    28681 gacaaagaga tttcttattt gaactctttc gtcactacac tagtttctga ctggatgaat
    28741 gaataccatg tctttttcaa aagaatcaac gcagaacggt ttttcttcgt agcacagatg
    28801 atcgatctga aaaagatgat ggataaaaaa tttgatttgc tggatcgctt tcgagcagaa
    28861 gctgaaaaac agagctcgcc tatcaccctt agtatgggga ttgcttatgg ctcaagaacc
    28921 ctcgatgaaa tcggtggaga agctcagaat aatttagatg ttgctttagt ccgtggaggc
    28981 gaccaagttg tcgtgaaaga ggtaaaagaa ggagcaaaac ctgtttatta tggggggaaa
    29041 tctgctaaga atgtcaaacg cacgcgtgtt cgttctcgag cgatgagtac cgctttgcgt
    29101 cggatttttg atgaaacagg cgatgtgttt atcatggggc acaagtttcc ggatatggat
    29161 gctttaggct ctgcttttgg tgtcagttgt ctggcacgct tcaaccagaa aaaagcttac
    29221 atcgttatca acgagaacga gatcattcca gatgtagaac ggtgtcttga agaaatccat
    29281 aagcatcctg aattagaaat gcaactaatc tctccagaac gtgccataga gctgaaaaaa
    29341 gaagaggacc tcctcgtgat ggtggattac cataaacctt ctctatcgat ctctcagggc
    29401 ctatatgagc aatttgataa gattgtgatc attgatcacc atcgtcgtgg ggaagagttt
    29461 ccaagtaaac ctctgctgac ttatatcgaa tcttcagctt cttcagcttc agagctagtt
    29521 tctgagctga tccaatacca aagcagcaaa acaaatcgct tagatcgttt tgaggccaca
    29581 ttattgcttg cagggatggt tgtagataca aatagtttca atgtccgtac aactgcacga
    29641 acttttgacg ttgccagcta tttaaggtct tgtggagcag acgcatcctt agtacaatat
    29701 ttattgagct cggatttagc gacatacttg gagatcagca atttgattgc tggaagcgac
    29761 tatgtgactt ctgatattgt tgtggcggca gggacagacg aaaaagagta taatagtgtt
    29821 atagcagcaa aaacagcaga tacattgctt tcgatgatca atatcaatgc agctttcgtc
    29881 attacaaagc ggacagacgg cttgattggg atcagtgcca gaagtaccgg cacgatcaac
    29941 gtccaaatca tcatggaatc cttaggtggc ggaggacact tcacaaacgc agctactcaa
    30001 ctggaaaata cgacagttga ggaaacaaaa cagaagctat ttgctgcaat caaagaaaat
    30061 atgaatcaaa tatatgataa ggagtaatcc aaatgaaagt aattttttta caagatgtaa
    30121 aaggaaaagg taaaaaaggg gacatcaaag aagttccagc aggttatgca caaaattatc
    30181 taatcaaaaa taacttggca aaagaagcaa cgaaaagcag tatcagtcag ttagcaggac
    30241 aaaagaaagc acaagaaaaa cacgaagctg aaatcttgga agaagctaaa aagttaaaag
    30301 aattcttgga aaaagaagat actgtagtcg aattgaaggc gaaagcaggg gaagacagcc
    30361 gtttgtttgg ttcgatccct tcaaaacaaa ttgctgaagg gttaaacaaa caatacaaaa
    30421 tcaaattaga taaacgaaaa atcgaattag aaaatccgat ccgttcacta ggctacacaa
    30481 acgtacctgt taagcttcat catgaggtga cggctacgat caaagttcat gtcgtacaag
    30541 aataacaaat cttgatagcg atgttcagct ggtaagtata accagaagga aaaaagacaa
    30601 accgaaattt atctgagaaa acagctgcag caatgaaagc aaaagctgca aatcgatttc
    30661 agattgattt cggtttcttt tttgggaaca gcaaaatagc ataaagaaaa cgagtataat
    30721 aaaagagaac tattttttta ttacactttc ggtacgaatg atgacttttc tctcattttc
    30781 ttgtaaaatg aagcaattga agacaaggag cgatacgtat gaatgaagtg tggcaggatc
    30841 gaatcccacc gcaaaatatt gaagctgaac aagcggtttt aggctctgtt tttcttgacg
    30901 cagaaacgat cattgatgcc atggagtaca tagagccaaa agacttttat cgtcgtggcc
    30961 atcaaattat ttttgagaca atgatcgagt taaatgatcg taatgaagcg attgacgtgg
    31021 ttactgtcaa agaccggctg gaacaagcca atttgttaga agatgccggt ggtttaagtt
    31081 atctctcaga cttggcatta gcagttccga cagcagcaaa tatcgtctac tatgcgaaaa
    31141 tcgttgaaga aaaatcattg ctgcgtacat tgatccagac agcaacagga atcgttacga
    31201 aaggattcga gcaaggagaa gaagttcaaa caattttaga tgatgccgaa cgcagtatct
    31261 tggaggtttc ggaaaaaaga aatcgaagcg gctttttatc tattgctgaa gtactgaact
    31321 ccacgattga aaatatcgat cgactagctc aaaacaatga agaaatcaca ggtctaccaa
    31381 ctggctatca aggtctggat aaaatgacag caggtttcca gcccgaagaa ctaatcattc
    31441 ttgctgcacg tccagcggta ggtaaaacag cgtttgcatt gaatattgct caaaatgtag
    31501 gaacaaaaac agatcgttct gttgcgatat ttagtttgga aatgggggca gaatctcttg
    31561 taaatcggat gctttgtgca gaaggttcga ttgaggccag ccatttacgt acaggtcagc
    31621 tttcggaaga agaatggtcg aatctgatcg ttgcgatggg gagtctttct cgagcaaata
    31681 tttttatcga tgatacacca gggatcaaaa tctctgaaat ccgtgcgaaa tgtcggaaac
    31741 tagcgcaaga aaaaggcaat ctaggcttga tattgatcga ttatttgcag ttgattgaag
    31801 gtaacggtaa agagaaccgt caacaagaag tatcagatat ctctcgccaa ctgaaaaaac
    31861 tggcgaaaga gctgaaagta cctgtcattg ctctttcaca gttatctcgt ggagtagaac
    31921 agcgacaaga caaacgacca gtgctaagtg atattcgtga atccggatca attgaacaag
    31981 atgcggacat cgtagccttc ttgtatcgag atgattatta tgaaagagaa ggcggagatg
    32041 acgatgatgg aaacgagcca aaaaatgaca acgtcatcga agtcatcatc gagaagaacc
    32101 gaagcggtgc acgaggaaca gttgaactac tgtttatcaa agaatacaat aagttttcgt
    32161 cgctttcgcc aagagaagaa ttttaaagaa gaggttgtga gccttgagca atcccgagag
    32221 ctctcgtaag aagatgcaag gagtaaccgc aggaacgact agagtactta atttaaacag
    32281 atctccaaag ggattgtgac aaaagtcttg tcacagtccc tttttccgaa taaacggtgt
    32341 tcaatcagct gacccttggt attagctcag caaacgtcaa aatatgagga actattttga
    32401 cgtttgtttc tctaatactc aggtcaaaac gctgatctta cgacctcttt tccttaaacg
    32461 gtgttctcct gccaactcct cggtcacaag tcacaatatc atcaaacatg agaagcagtt
    32521 ttttgaaatt gctccttgtg actcagagtt aaacggcagg ctcacaacct ctttcttaaa
    32581 cggtgttcaa aagcttgcat ctgcggtaat acgcccaatc aaacgaaaaa tatgagaagc
    32641 tatttttgtt tggctgttct tattacttga tgcaggacag ctttttcaca acctctgatt
    32701 cacggtgttc caaaagtagc ttcttcgaaa aataagtcga aatttcacaa aaatttgaga
    32761 agcaattttc gtacattcct tcttatttct tgaagctgac cacttctgtt acaacctctt
    32821 ttttaataac cattctaaaa agataaagtt cgttttttaa gcgtttttat aaaataaaaa
    32881 taaaaatcat tcgattttat ttgaaatatg tatctattat tgatacaatg gttcaggatt
    32941 aaataatata gaacgaggtg ttcacatgtc atctgtagta gtagtaggta cgcaatgggg
    33001 cgatgaaggg aaaggaaaaa tcacagactt tctcagtgaa aatgctgaag tgattgcacg
    33061 gtaccaaggt ggcgacaatg cagggcacac aattaagttt aacggtgtga cttataaact
    33121 gcatttgatt ccttctggga ttttttataa agacaagatc agtgtgatcg gaaacggcgt
    33181 tgtagtcaat cctaaatcat tggttaaaga attagcttat ttacatgaaa aaggtgtaga
    33241 aacaagtaat ttgcgtattt ctgaccgcgc gcacgtaatc ttgccttatc atatcaaatt
    33301 ggaccaatta caagaagatg ctaagggcga aaataagatt ggtacaacga ttaaaggaat
    33361 cggacctgcc tatatggata aagcagctcg tgtaggtatc cgtattgcag acttattaga
    33421 taaagaaatt tttgaagaac gtcttcgttt gaatctagat gacaaaaacc gtcaatttgt
    33481 aaaaatgttt gattcagaac cacttgattt tgacgatatt tttgaagaat attatgaata
    33541 cggacaacaa atcaaacaat acgtcacaga tacttccgtt atcttgaatg atgctttgga
    33601 tgcaggcaaa cgcgtgttgt ttgaaggcgc acaaggtgta atgctggata tcgaccaggg
    33661 aacttatcct tttgttactt cttctaaccc agtagccggt ggggtaacaa tcggaagcgg
    33721 tgtagggcct tcaaaaatca ataaagttgt cggcgtctgc aaagcctata cgtcacgtgt
    33781 cggtgatgga ccatttccaa ctgaattatt tgatgaaaca ggagaacaaa tccgcgaagt
    33841 aggacgagaa tacggtacga caactggtcg tccgcgtcgt gtaggctggt ttgattctgt
    33901 agttatgcgc cattctaaac gagtttcagg tattaccaac ctatctttaa actcgatcga
    33961 tgtattaagt ggattggaaa cagtaaaaat ttgtacggct tatgaattag atggcgaact
    34021 aatctaccac tatccagcaa gcttgaaaga gttgaaccgc tgcaaaccaa tctatgaaga
    34081 attgccaggc tggtcagaag acattacagg atgcaaaaca cttgcagaac tgccagaaaa
    34141 cgcacgtaac tatgtacgtc gtatctcaga gcttgttggt gtacgtatct caacattttc
    34201 tgtcggacct gatagaacac aaacaaacat cttagaaagc gtctgggcgc aaatctaagg
    34261 ataaaaacga cgactgaatc aggacgttct taccagagcg agtcgaagtg tttatctata
    34321 cgataataat aacttgaaga ggtagtgaaa gagacgttta gccttattgc cgataggctg
    34381 tctcttgaac actgtttatt cagaataagg gactgtgaca aaaaccgtgt cacagtccct
    34441 ttttccgaa
//
LOCUS       DAYYGO010000017        32271 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000017 DAYYGO010000000
VERSION     DAYYGO010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.017"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..218)
                     /locus_tag="ACHA5T_000702"
     CDS             complement(<1..218)
                     /locus_tag="ACHA5T_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886302.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKIT"
     gene            complement(225..773)
                     /locus_tag="ACHA5T_000703"
     CDS             complement(225..773)
                     /locus_tag="ACHA5T_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFM8886303.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            complement(818..1150)
                     /locus_tag="ACHA5T_000704"
     CDS             complement(818..1150)
                     /locus_tag="ACHA5T_000704"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886304.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(1164..1421)
                     /locus_tag="ACHA5T_000705"
     CDS             complement(1164..1421)
                     /locus_tag="ACHA5T_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886305.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(1616..2158)
                     /locus_tag="ACHA5T_000706"
     CDS             complement(1616..2158)
                     /locus_tag="ACHA5T_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF0163 family protein"
                     /protein_id="HFM8886306.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            complement(2179..3270)
                     /locus_tag="ACHA5T_000707"
     CDS             complement(2179..3270)
                     /locus_tag="ACHA5T_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8886307.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(3278..5488)
                     /locus_tag="ACHA5T_000708"
     CDS             complement(3278..5488)
                     /locus_tag="ACHA5T_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFM8886308.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(5504..8032)
                     /locus_tag="ACHA5T_000709"
     CDS             complement(5504..8032)
                     /locus_tag="ACHA5T_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8886309.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            complement(8084..8494)
                     /locus_tag="ACHA5T_000710"
     CDS             complement(8084..8494)
                     /locus_tag="ACHA5T_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFM8886310.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            complement(8507..8725)
                     /locus_tag="ACHA5T_000711"
     CDS             complement(8507..8725)
                     /locus_tag="ACHA5T_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpD family membrane protein"
                     /protein_id="HFM8886311.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(8722..9714)
                     /locus_tag="ACHA5T_000712"
     CDS             complement(8722..9714)
                     /locus_tag="ACHA5T_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8886312.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            complement(9793..10704)
                     /locus_tag="ACHA5T_000713"
     CDS             complement(9793..10704)
                     /locus_tag="ACHA5T_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM8886313.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(10723..11067)
                     /locus_tag="ACHA5T_000714"
     CDS             complement(10723..11067)
                     /locus_tag="ACHA5T_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886314.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(11087..11365)
                     /locus_tag="ACHA5T_000715"
     CDS             complement(11087..11365)
                     /locus_tag="ACHA5T_000715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886315.1"
                     /translation="MSVEHFCDCYIALLRSQQLLTVERYGAEFGYVLQAERHRATRCY
                     EKEDQVIDGYEELCIRNNRYLQQRLYEQLMMDFSNLYRGYECSMIQER"
     gene            complement(11724..12224)
                     /locus_tag="ACHA5T_000716"
     CDS             complement(11724..12224)
                     /locus_tag="ACHA5T_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886316.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            complement(12225..12770)
                     /locus_tag="ACHA5T_000717"
     CDS             complement(12225..12770)
                     /locus_tag="ACHA5T_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886317.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(12767..14023)
                     /locus_tag="ACHA5T_000718"
     CDS             complement(12767..14023)
                     /locus_tag="ACHA5T_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM8886318.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            complement(14384..14695)
                     /locus_tag="ACHA5T_000719"
     CDS             complement(14384..14695)
                     /locus_tag="ACHA5T_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886319.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            complement(14718..16193)
                     /locus_tag="ACHA5T_000720"
     CDS             complement(14718..16193)
                     /locus_tag="ACHA5T_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HFM8886320.1"
                     /translation="MKAHFFKYRGRRIRYFNRYLWFCYYGFFLFPFLILLGWFSWKVF
                     YPRLSTFSNWQMYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLVRML
                     IDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGHLLS
                     EVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPNMLI
                     AGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEEMII
                     CLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDMEARN
                     RVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYLMTF
                     GEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYDFTV
                     KEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            complement(16642..17094)
                     /locus_tag="ACHA5T_000721"
     CDS             complement(16642..17094)
                     /locus_tag="ACHA5T_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886321.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            complement(17096..17461)
                     /locus_tag="ACHA5T_000722"
     CDS             complement(17096..17461)
                     /locus_tag="ACHA5T_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886322.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            complement(17560..21843)
                     /locus_tag="ACHA5T_000723"
     CDS             complement(17560..21843)
                     /locus_tag="ACHA5T_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HFM8886323.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            complement(21990..22229)
                     /locus_tag="ACHA5T_000724"
     CDS             complement(21990..22229)
                     /locus_tag="ACHA5T_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886324.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            complement(22319..22561)
                     /locus_tag="ACHA5T_000725"
                     /pseudo
     CDS             complement(22319..22561)
                     /locus_tag="ACHA5T_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708076.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(22674..22910)
                     /locus_tag="ACHA5T_000726"
     CDS             complement(22674..22910)
                     /locus_tag="ACHA5T_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886325.1"
                     /translation="MFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTELKDKN
                     NVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            complement(23921..24831)
                     /locus_tag="ACHA5T_000727"
                     /pseudo
     CDS             complement(23921..24831)
                     /locus_tag="ACHA5T_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(25027..26862)
                     /gene="lepA"
                     /locus_tag="ACHA5T_000728"
     CDS             complement(25027..26862)
                     /gene="lepA"
                     /locus_tag="ACHA5T_000728"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFM8886326.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            27009..28229
                     /locus_tag="ACHA5T_000729"
     CDS             27009..28229
                     /locus_tag="ACHA5T_000729"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8886327.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            28249..28743
                     /locus_tag="ACHA5T_000730"
     CDS             28249..28743
                     /locus_tag="ACHA5T_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFM8886328.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            28721..29407
                     /locus_tag="ACHA5T_000731"
     CDS             28721..29407
                     /locus_tag="ACHA5T_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886329.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(29558..32167)
                     /gene="clpB"
                     /locus_tag="ACHA5T_000732"
     CDS             complement(29558..32167)
                     /gene="clpB"
                     /locus_tag="ACHA5T_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFM8886330.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
BASE COUNT         9383 a         6861 c         4998 g        11029 t
ORIGIN      
        1 cttgttattt tttcattgac tataactagt tcatctggta aaaaaattaa ccaacaaaca
       61 tcgacaaaca gtaaatgata taatccattt ccacctccaa gcatattcaa tactggtgaa
      121 catgccttag aaacacgaat atagttatgg tttaaataga tattttcttt aaacattgct
      181 tgtttaaaag ccatatgctc ttgttctaaa cttttcataa acttttactc taatgcacag
      241 ctagcagcag ctgctgcaat tcctgctaca atatttacct tagctaactc tttagctgca
      301 gcttttccta attgtttttc tgcaattttt aataataaat ccgcagcttt ttcaaaatta
      361 aaagctttta atgcttcaat aaaatctccc tgcgtaccta tagcccaagc cactcctcct
      421 ccaggaagag aattaatggc tacacatcca gcatatcgtt tccaatcaac tttctccatt
      481 ctaggaacaa taatatgttc ataaaagact cttgctgctc ttccttgttc gttatcttga
      541 ctgattcgtt cacgaatagc accaaaatcc tttatatggt atacaccatg ttcgtctaaa
      601 aaaccaactg tatcaaaata aaaagttaat tcatctacta atttattctt tatttctaaa
      661 tcaactgtat tcactttttc tgtggcatac acacttgttc cagtactaac aaaagtactt
      721 aaacctaata agaaaacacc taaaacacca aatattttaa ttattctttt cataatttct
      781 cctccataaa aaataagtta ctttttaaat atcttgttta tgaattcatt ccccaaaggc
      841 cttttaagat tgatacagca gtccgttcac ttcttttaga cttgtcatac aagtctttta
      901 aatgatccca tatcagacta gtttgtctat ctagcttcaa tccattaaac gctaatgaac
      961 ggttcatttt ttctattact ttagataaag ctacagaaat ttttatacct tcacaacgcg
     1021 catcttggat ttcaataata gtatcatcaa ttagtttttg aatttcttgc ctatcaacat
     1081 attcaatctt agaaagagaa tcttttaaag ctactaatga ttcaacgatt tcttttttct
     1141 cttcagacat tttcttcctc cttctagtga cccgtaaagc catgtagagc agagtccatt
     1201 gaaccagtaa ttacactatc tacaacgcca ttccaattta ctctacattt tccatttttc
     1261 agatttgtcg gtccatacgg atagcctttt tgacattcaa tgttcggatt ataccagata
     1321 ggattatacc attcagccgc agaagcagat tcagtcgata caaacaatcc cacaccaaca
     1381 aatccaatac acatcacact atagccaatt agttttttca tctcgtatcc atctcctaaa
     1441 aaatagaata gttcttatag ttattcataa gacatatttt ttccgttttt gttttatcct
     1501 ttctgtccta taacaacttt tttcaagtaa agaatgacat agtttttctt aaaaaaacag
     1561 tctgtttttt tctatttctt cggtgtataa taaatatgag tgttcatttt ttattttaat
     1621 acgcactccg tacataataa atcttgatcg catcaatctt ttgagcttct tgatctactt
     1681 tcaattgaag taatacaaat tgagtaacct tattcattgt ttcttctcca aataacaaat
     1741 attgatccgg attctctata tcttgtgtca cctggtaaag tttcccagtt gttttcattt
     1801 gtacatgcgg gtctacatca atactattgt ctttaataca acgatctgtc atatagtccc
     1861 tcacagcttg attgcgttta taaacatctg tgaaattact ccattttcta gcaaaatctt
     1921 ttaagaaatt ttttgtcatt tcatctattt gcatttgttg cgctgtctcg tcttctaatg
     1981 aaccaatttg actgttactt tcagtacttt gaagagttcc acttgtttct tttactgctt
     2041 cgattgtttc ttctgtcgtt ctcaattgtg ttttttgttt ttcagaagaa aaccaatgac
     2101 aaccagtaag acctatcaaa cataaccaac taaatactaa aattatccct tttttcacgt
     2161 ttctagtcac tgcctttctt atttcacctt acgaaccaga ctacgaattt ttgtttgatt
     2221 gtacgtgcgt tgataccgag ccataatccg tccttgctcg gcattctgtt ccacactaga
     2281 gaaatgttgt ccgccatatg aaacatctgt tataattccg gtgtgaccat agattccagg
     2341 agacaatgca ccacctgctt gccaattcac cacatctcct gcttgtaaat cacttggttt
     2401 tgggttcatg attacttgcc aaccataggt tgtccagtta taatcttccc ctattaacat
     2461 cgcaaacatg tgtccagaac ccattaactg tgggccacct aattgagata cataccatgc
     2521 cgtaccacca taacattccc cattatttac ggatcgtcca atgatccgat ccaaaacctt
     2581 cgcattgcct ttactttttc ctgcaataag cgaagttttt tttgttggta ggctattcca
     2641 atgcttgatc gcaaaagtat catattccgt taagttatag gacgttatga tactcattgc
     2701 tcctcgataa tacggactcc cttcggtaat acctgctgcc gcatagcctc cacgacctag
     2761 aatcaataaa caacttccag gatctttatt gcccacacat tgattaaagt tgctttggct
     2821 cgccaccata aagccataat agtcataaca agcttttaag ttcggaaact tcatatagaa
     2881 accaccttca gaaccaccaa ttccacgtgc agaaccttgt gggtagggac aaccaggaaa
     2941 ccaggtaatc ccaaaaaagt tcgtatcgtt tcttccagat tgtgactgtc cataagctga
     3001 ttcatagcct aattgagaaa gaatcacact cggtaataac tgatacttta cagctgcacc
     3061 aactgctgct tgcgcatgtt catctgtgat tggaccacgt cctttaatct ctgaaaaaat
     3121 cggtaatcct tcggtatatt ccccattaaa agcgacagct agacctgtag cttctcctcc
     3181 atgctcagaa agttctcctg aaaaaaagaa tggcaataga aaaaagggaa gaataaagaa
     3241 aagaagacaa cctagacacc ctttcttcat ttcttcctca tcctttcttc gataatggtt
     3301 tttggactgt tcgtcctttt ggtaaagtaa aatcagttcc tggttttgcg tccaaaatcg
     3361 gcagtgtttt aggatccttt tctatttttt ccgattgctc tgttggctta tctaaagaag
     3421 aaaatgcagg ttgtaacgat tttggtgcgg aaatcgacgg tttactttca gacgaacgtt
     3481 gccccggttg ttgcacagaa cgatttcctg atagagtggc taccgacgaa ttaggaacag
     3541 agtattggtt caataactgc tgcattgctg gataactccc atttttcgcc tgatttttca
     3601 cttggcgaat ctgttgtgaa agagaaccag cagctgttaa ttgtggcacg ggtacttttc
     3661 cagaaggcaa cggaatcgat tgaccaacaa aagcgatacc tttatttaac aatggttttc
     3721 tttgattatt cccttgttgc ttactcaatt gtgccaatga ttgtacctgt tgttttggtc
     3781 ttactgaatt catggacgaa tttggaatca aaccatttga aagaccagaa ttccactctg
     3841 ttttcgattt ggtagaagac atttcaggct ctaccccatg ataacgaaca tgacgaccac
     3901 tataaggcaa ggcttgattt gaacgcccac ctagttcaaa gggattaacc acttgcctcc
     3961 cttttaccga ttcttgtggt gtacgttttt gttgaaactt ttgaaatcca tgtatcgctg
     4021 cagaagcgag tggattcgtt gctcgataca cattccctgc taatcctgca cctttgattg
     4081 ccaaattttg tccagcagtt gatagttgac caggcagttg tttcatttga gacaaagaat
     4141 gacttgctat gtttttcgtg ttgtttatag tttgatctgc agctcctaac cctagtttct
     4201 ttaataaatc tttccgtttt acaatgagta aaattgatag gagtgctttg acaaacatat
     4261 ttcctaaaaa accaaaaaca gtattcattg gaattaaatc gtctactatg ttatagatca
     4321 aaaatgccac caaaataatc acactgtaaa aagatttttg aaacatcaag cccaaaaagg
     4381 actttaaggt attcatgagc atataatcaa atactggaac aaaagaagcg agtaatgaaa
     4441 aaggaatcac aagtaataaa cacaacacca acatttgaaa gataaaacga atcatgccaa
     4501 tcactacaag tggtaatcca atacttagat taagcaatgg caccatagaa ccaacgactg
     4561 ctttataaac agctttagtt ggttgaatat agacacgata agccttcttt ttccctctat
     4621 cttctcctgc agcagtggtc acttttgtct caatatcaga taattcttca cttgacgctt
     4681 ccttggctag aaattcttgt ggttcaatct ctgcttgata caattcttct aaatttgttg
     4741 tcccataatt taacaatata tacggatcaa caacagccgc tttataataa agttggcgca
     4801 ctttttgaat ggcttccttt tgtgtcgttg gcactaaaac accattgaca tcttttgtcg
     4861 ccaattcatc tagactattt gtagcagaaa aaataaagcc ttctacctcc acggacaaat
     4921 catttaaccc tttggtaaaa gcttctcctt tactaggtac gttactacca tagccaaacc
     4981 aaaccattga aaaaatgaca acgaagagaa atcgaataaa caccattttt gcgtaatgtt
     5041 tccctctaaa tatacgaaga gtaaatagat ataacacata caagacgaca atccccgcac
     5101 caataggatc gtatattttt gaataaatag tatccgaaaa agtgaaaaac tggtgaatta
     5161 actcgttcat tgcagctcct tcatagagct tctcaatgac aaaatcaagg ccttgccaaa
     5221 tcaatttact taaactaaag aacatattcg tcatcatttt tagaaaatct gtgccatttg
     5281 ccccaaataa cattttgcct aatgaaaatt cactatcaga taaataagaa gaaaaggtat
     5341 caatatgaaa tttcccaatc tcatagatcg attgatccac ctgcttttct gccgcataca
     5401 ccaatccacc aacaaaaagc agtccgaatc caatcgttaa aaaaaacaaa acgcaccatt
     5461 tcttttcctt atgctttata taagccattg tttcacctca tttctattgc atgattaacg
     5521 tatcctgttc ttctacttga gagtgacttg ttttttccgt tgtttgtaat gcagcggtca
     5581 tttcttcaaa caaactgtgt acattgagtt ttcctacacg tccataggga tctaacatcc
     5641 aacactgtcc tttaatcatg tggctcattt cagctaagtt cgcttcattt acttccaaac
     5701 gtaaatgatt caaaatcttt tctcgctcac tgtcttcatc aaaagcaaag acgacaccaa
     5761 aattcccttt ttctttatct gaacctaagt caccaataaa ttgtgtatct aacactaatt
     5821 gattcatctg ggaacgcccc acacgtgcca ttgctgcaat aatactacgt cctgcgttcg
     5881 aattattaaa tacccatgct tctgtaaaat aaatcgctgt tttcttacta ctatctcgtt
     5941 ttccaaacat ttcacaaaaa cgacctaaac taatcatcaa acacaaagac tttcgatccg
     6001 catctgtata tagttctggt cggacattgt catttggtaa tttcaaatct tggatctcta
     6061 aaatcgttgt tttggcatta aagtctaatc ccgggttttc tccataagaa aatcctaaac
     6121 gtaaaatcga atcttctacc gtcaagcgta aaaactcacc atattctcga acctcttttt
     6181 gttccatttg ttctaaatag tcgaccacat gcatcaagcc aacttgttgt ccggcttctt
     6241 tttgaactaa aacttcttgg attcccttta ataccgcccc ttttaaaagg tgattggtac
     6301 ctaacggact taactcatca atcatatcct gggctacttg tttcgcatct actcgggata
     6361 aacaaacaat tggatctaat acaccataat tacggggatt tgtagcatct aacgtgacat
     6421 aactcatttt ttgcaaatgt tctacaaaca aagggtaatg tttctgataa tacgggtctt
     6481 gaatgagttt cgtaaaccat ttgcgctttt ctcctttggg atccacaaat aaactttgta
     6541 cattcataaa cgaactatac aaaaagagca agccaactaa aaaggtcttc cctttaccgg
     6601 tttttccagt tactgctaca tgaggcgcat caaacaacgc acctttgatt ccttccgcaa
     6661 tggctagaag attcaaaaag acaaaacgat ttgacgcata aattaattct tctaatggtg
     6721 ttttttctcc tcgagaacca ccatccccta atgtatcttg ccgaccaata taccagccgg
     6781 attccatacc taattctgat actgctgaaa acaaccattc gccaactgct tctacagttg
     6841 ttcggtgctt ccaatgtaac tgccgtccca atggttgccc taataaacat ttatagaaca
     6901 attctcgttg atcatttttt gcacggtgga tatggataat cgtatgaagt tgttgaataa
     6961 ccgttgcact attttgacga acttcttcaa ccgtttgacc gtatacaata aaacatgcga
     7021 tccaatcgac gacaggttgc ccattatcta ctcgttgctt taaatgtttt actaatcctc
     7081 gaacagttaa taagcgttca ctaggttctt catcattttg aaccaattcc ttatgctctg
     7141 ttttgaaccg ttccgatagc caattcagtt ttaaaccaat acctccctgt cctttcaagc
     7201 gtggatagtg aattttcatt cgcaattctg ctgggaaaga aagatcctga gcaaattgaa
     7261 atagatcaca atttctcata ttttcaggtg tttccccaat aggcaacaat gctaaataac
     7321 tactccctaa ctcatcttct aattgaagaa ggcctttatg ttccttcggt atacgtacat
     7381 acgttccttg attgtctttc agtacttccc aattattttg ttcttctact ttgtgagcca
     7441 ttcctcgaat atattgcaga cgattgagat acaacagctc tttttttact aagggacgaa
     7501 tagaaaagct ataaagtaat tgtgtcactt cttttacata aggatcactt tcagaaaaca
     7561 aagtttccaa atcatctact tctaatccaa ataagcgtgc taaacttcct gctaaatcta
     7621 accagattag atttattaca tcatgaaaac tctccaccgt tgctttttct ttgactttga
     7681 ttcctaaaat aaatgaagga cgatagactt gaccttctcg ttccaacaat gtcactgttt
     7741 cttttgcata atacgcacca attgcttgac tatcgggaga aaatcccttt cctaatgtgt
     7801 caaacttcgt ccgtaaattc attgttcgtg gatacatcac caactccaca tcttcataat
     7861 ctgctaaaag caacagtaaa cgagccatca tttcttttat ttcctgtgtc ttttttggtg
     7921 tgttttttga aattgttcgt ggaaaaacat gatagtaggc ccagatacaa tcctctccat
     7981 ttaacaaaca atttttcttg aatgctttta ctggttcttt tacaagcacc aatgaatttc
     8041 acctcccaat aaaaaaggaa caaccagttg attgtccctt tttctaatcc tctaaataaa
     8101 catacttatt cacaaaaagt aattcttcca atgcattgtt tgataccttg tcccagttat
     8161 aatacacaca atcaccattc atcatacgaa tcatataaca aaaccaatcc cataaataaa
     8221 aatgcacttt ttttccatca ggttttagtt ttgccagtgc taacacacat aaatagggga
     8281 gtcctatata aaaaacaact cctgctggtg gaaaaaaaga aatgacccaa ccaataaatg
     8341 aacgtaaaaa taaatattca aagattacga ccaacaaaaa agttgctgca ttgactaatg
     8401 caactggaaa tggaagacgc caattgcttg taatctgttg tacgcgataa ggttccgcat
     8461 aaacaatcga ataatcataa ggttcccaat ccacttcact atcctcctaa tccataaata
     8521 atttttcaat gattttacca aacgcatcaa atacacgtgt tggatcattt aagaagaaat
     8581 aaactgcggc acctgctgcg atagatatta atgcatttcc tacttttgat ttcataaaat
     8641 ttcgcaatcc cagataaaca accaccatta ttaaaaaata gccaccttgc gttaatataa
     8701 aatctacaat tcccttcagt gtcatttccg ttcttctcct ttcctatcca accaattatg
     8761 tgttaatttt tcaacataat actgatttgt ttttttcgta atgacaatcg tcatttgttc
     8821 tttttgtaaa agtgacgtat caccatctcg aaagacgacc gttgctttta caataaaagg
     8881 ttttttcgtt tctttataac gcaatacttt catctctact tgatcaactt tagctgtttg
     8941 attgagtcct tcaggttctt tcatcatgta tgacatatct tttgcattgt ttttcgcata
     9001 cttatctaag aaatcttcca caaatccttc aacatctttc gcttccgacg catcaaccgt
     9061 ttcgtataat gtgtcatcat aagtaatcat tcgttgtttt tctgtattct ttggtagtgc
     9121 cgtaaaataa ggataagcaa taatacgaca gcccttgttt gaaatagaaa agggaagatt
     9181 gagtacttgt gtgattgtca cacttgtttc tttcttactc tctggattca tcgagcgaat
     9241 atcataagtt actttataac tagcagttgg aatggtttca atttctttta atccaataaa
     9301 cgaagcagca gttaattttc gaacacctga aacacttcct ggttcttgaa taaagttttg
     9361 tgcataaaac tcttttttta atgtttcttg acgttgttgt tcttgttcat gattatcgaa
     9421 tgtattcata tacaaagcaa caaaacgatt cataaaggat tcaatttcag gtgtgtacgt
     9481 taattctgac cgactttgta cttctttctt ttgtaattgt tggatttcat ctttcaaaaa
     9541 cgacacttga accttcgttt tcatggattg aaaaaatgcc aagccccctg aaaaacaaag
     9601 aaataaaacc aaacaccaaa tccatttcac tgcttttttt gaatggattt tcttttgctt
     9661 cggtactttc tctgtataaa ctttttcttt ccgccgtagt tgtcgttttt tcataagcta
     9721 ctccttctag tatgttttta ctagtacgta cgattgacaa cgataagctt gtttttctga
     9781 aaaatagtcc attcaattac cctcattttc tattcctttt ctctttcttt tatatccaac
     9841 aaatccagca atacttgtca acactcctcc tagaacagaa agtgcaacat gtatttcttc
     9901 accagtcttt ggtaagactt ttgtttttcc atctgctgtt ttgatttcaa tattgccact
     9961 tggaagtttc tttacaggaa tcgacaaatc ctttaccaaa ttcccttcac tatcttggat
    10021 taatggaata ccatctttta caccaacaat tgtttgatca cctgctactt ttaatggggt
    10081 ggttggtgtt ggaatatggg tcaattcctc tacattacta gtgacaatcg gtacactcat
    10141 tccttgagaa gtattcgtcg taatttcacc gcttggggtt accgatactg tgacttcttt
    10201 tgctggaaca ataggtttat tttcttttgg aattgcttct gatttttcaa caggttgttt
    10261 cggttcttct ttttgttctt cactggtaga cgacatcgtt ggctgttccg tacttgacga
    10321 atcagtattc ttttcagaag aactggttgt tcctgattgt tccggagttg tgactatact
    10381 tgaatccgtt gttgttgaac tatgcacacc ttcttcttcg atcgaatcag tcggtaaagt
    10441 tgattcagga aactctggtt ctggtgtcac tggctctttt tcaggtttct ctgactgcaa
    10501 ttgttcaggt tctgctggtt tttccgttgt tcctccattt agctctgtct caacatctgg
    10561 taatccttcc cctggcaatg tttctacaat ttctgactta ggtgtttcta cagtttcatc
    10621 tgcataaacc aatgaactcc ctaattgaaa cgaaaataat gaaattccta gtgttgttac
    10681 gactgctaat gttgtttttt tcatgattat tcctccaatt ttttatgcaa ttcgtgtttt
    10741 gttcatagac gccgcactaa tattatctga acgataaaag aactcccgtc cgttttcttc
    10801 taacccaaca tatggattat ttttgaataa ttgaatcaat agttcactgg tgttgtattt
    10861 tggatagttt gcaaaaaaat aatactgtgc ttgtcgccca tccgaaaatt caacaagtaa
    10921 ttcataacga taccgcttca atccttttcg ttgttttaaa ggatctctat accaacttgt
    10981 ccatcgttcc attcgtggat agagatagac acgatacaca tagaacaatg ccataccaca
    11041 taccaccaaa aataataatc gccacataaa aattccccct tttcttctac ctttcttgaa
    11101 tcatcgaaca ttcataccct ctatagagat ttgaaaaatc catcatcaat tgttcatata
    11161 aacgttgttg caaatagcga ttgtttcgaa tacataattc ttcatagcca tcaatcactt
    11221 gatcttcttt ttcatagcat cttgtagctc tatgacgctc agcttgcaag acataaccaa
    11281 actctgcacc atatcgttct actgttaata actgttgact acgcaataat gcaatataac
    11341 aatcacaaaa atgttcaacc gacatttgat ttttctcacg tttgtgaaat aggtattctt
    11401 tagaaagcac attattcgtt aaacaatctg ataaatagcg ataatctggc ggtcggttat
    11461 tttcatctgt gaatttttcc cataataaac tgatttttat tcgttcagcg gctagatagt
    11521 cttgttcgcg tggataaaat tcatcaatcg cacaaagttt tttatcacct gaataaacaa
    11581 atccatataa tgcgaaacga actcttcctt ttcaattggc tctcctaaaa attccagtga
    11641 cataccatct tctccttcct aaaaaaaata aaaaagcaga aaccgatttg gcttctactt
    11701 ttctttcctt ttatagctct tttttacgga agaatccgca aatgacattt tttcaaaaaa
    11761 tcaatcggca caagttcacg aaaacttgga gcaattttgg atagaatcac ttccccatct
    11821 tgtaaaatac aatcataaaa atcatcagtt acttgatact tacgcttctt acaataattg
    11881 ataaaatagt agcgatcatt ttctatttcc attcgttgtt ctactaagcg catacgaaca
    11941 tcatcataga aataagccac aaatcgccct tcaagttcat gtttttcaat gggatactct
    12001 tctggtaaca atctattttt tcgtcgttta tacattgctg gataccttag aagaagttca
    12061 tctaaacgtc tgtcagtata tggtaactga cgaatcaacg attctaatcg ttctttatac
    12121 agatagaata attggcgcaa gcaatagtgt accgcctctg gattttctaa tactgttgga
    12181 tcacgtgacc aatacgcacc aacataaatc ataatttttt tcatttagac aatcacacca
    12241 gcaatactaa tgaccgatcc acaataggta gaacaagtgg catagcgacc aatttcctct
    12301 aattctatct caatctcttc taaagaaaga tatgtctgtt cttctgttgg ctggcgactc
    12361 atttcccaag tgacagtcac caatgaaatt tcttccataa gaggtttttc gctgctaagt
    12421 gctgccacac agttttcata tatcttcatt ttatcgcagc cttttttaac cgctaaaatc
    12481 gcaggtattg gcaatgaaaa tcgataaaag cgaatgaatt ctagccttcc tttccttcgg
    12541 ctaaattcca actcttcgag ttcttgttcg tatcccttac gaacttctaa taagcgtgca
    12601 atttcttgcc agttctcttt ttcctgataa cgtccagctt ttaccgcttg ttcaatataa
    12661 ggaactcgaa aacgaaaata agacaaatca accatctttt catttaatgc ttcgattgca
    12721 gcctttaaat ccaacaggcg cagccattct gttgtcgtat atgcattcat gcaattttcc
    12781 ctcctgacgt tcccatccat tctagatagt tttgttgctc gtcatttagt tcggcattat
    12841 caatcccttc ttgtacaaag tctcttccta aaatacgatc tgcttcttgt aacaacttta
    12901 atgtcggagc tacttgtttc aaaaaccagc gttctgaacg atctaatgag aatttttctg
    12961 gctttactgt aaaatccaat tcttccatcg aacgaaataa ttttgcccat cgtttatcaa
    13021 caatccgtgc atcactttcc agtctatccc aaacaataag ttttgcatta attaaaccaa
    13081 cgccaatctc tcctaaacgt ttcggtgaat cacctaaaat aaatgcttct actgcttgtg
    13141 ttgctttctc attggctaac cgtaactcat atcgattata aataccatat ttcgttaatg
    13201 cttctaattc tgaaattttt tctcggttcg caatttcaaa acgtttctca taaaaattaa
    13261 aaaagagagg actctttcgc gatccaaaat acaaagataa tccttcactt cgaaaatagc
    13321 catcatctaa aaaacaaccg ccaatattct ggaatctctt aaagtccatt tctaataaac
    13381 ctgctttttg tttttctagt aattcaaaga gatcgaaatg ttcttccgtt tccaatcccc
    13441 aaagttcatc taacgcaata tcgaatcggc tacattgcac acgtgtataa ccagcttcca
    13501 actttccttg atttttccaa atggaatata gccatgttcg ccaatctgtt tgttgttcac
    13561 gcatgacttc ctcatattcc cggcaacctt gtcctgaaaa ttgcacaagt acattttctg
    13621 ttacagttcg ttttgaatgc cagaacaccc agatattccc acgaacagaa agacagtcaa
    13681 aaagatacaa gcccgttgga cgtgtttcaa attctgtcag atccattcgt aatacacgtt
    13741 gaatcactgt tttgtagtct gtcccaaaaa aggaaagacg caaataatca atcattgctc
    13801 gaagatctat tccgcttttc atatacaatc gaaagcgttc cttgattcgt tgttcaaaat
    13861 cctctgggat ttttcttttc ccttgttcca tctttctaat gtaatctttt gagcagccca
    13921 tcaattgcgc aaatcttgct tgtgatagcg gctttttatc tccacttata ttatgaattg
    13981 cacttaactt ctttctgtag ctttttagct caatcggtgt cattttctcg ctccttttat
    14041 aaaataacaa aatcgatttt ttttaaccct ttagaaacac cgatatatca actagtcccc
    14101 ctcgtttttt gggaaaagta gtacaactgc tgaccttaga taatcacttg atacaaaagg
    14161 gtttataaaa cttttttcta tgtgttatgt tatttctgcc ccccatatta tatatcgggg
    14221 ggctttacac aaagaggccg tgcgcaagcg cacgacctct ttgtgagctg cacacgctgc
    14281 tagcgagtgc gcattatagg cccatgcttc gcagccttaa tgcgcaccgc caggcagagt
    14341 gcggctacca gactacgtct agagccctcc cgatagataa taactacaaa attaaatgat
    14401 ctaattttgt tatttcacta tatttttcag gagaaaaaac gcgataaatt gacaaagtta
    14461 aatcctcttc taaatctact cgataatgat acaatcgagt caatcgttct atatcttttt
    14521 tcgttagcaa tacttgatta ataacttttc ctgtaaaaac acgttctttg cgcaaaaagc
    14581 ggtaatcagt tacttgttca atacgattca accaatcata aaacgttctg gaagaaagag
    14641 aaggattaat cattcgtatt gctgtagcta ctctataata acaactctct gccatttaac
    14701 ttcacctcct tagttgatta caaaatatgc gcatcagaaa aacttgttcc ttttggatct
    14761 tttgctaaat acatttggcg ctctagttgt tcttgtaaca actgattatc atctcccatc
    14821 gtttgatctt gactatcctc ttttactgta aaatcataga caatcatttc agtaaatttc
    14881 tcaaattctt tgataaagtg ataatctttg taaacaaatg gcgcaaaaaa ctctacaggt
    14941 acatcaaatc ctggaaaagc atatccacga ccaggttgat ctttgttgta aaatggctta
    15001 tttttaaaat cttctccaaa agtcattaaa taaccaatgg gagacaatcg accaacagaa
    15061 atacggaatg ttaaattatc ccgaacacca cttggaaaat attctgcatc tggtcgttgc
    15121 attgctaaaa taatgtacat ccctgcttga cgacccttta atactaatgg ctgaatcaat
    15181 ttatcgactc tatttctcgc ttccatatcc aacgaaccat aaaaagctgc ccattcatca
    15241 aaaaagataa aatgcggtgg taacccataa taagcataat ttttccctgg ttcatattta
    15301 ggatctttca tggttttcat ataggcatac cgttgattca tttcttttaa tgcatttcgt
    15361 aaacaaataa tcatttcttc atttattttg taaaacacat gtcctttgaa tacatcgacg
    15421 tcacctaatg ctgctaaatc tgccgctttt ccatcgcaaa tatctactgt cccaacactt
    15481 aaacaagctt gaattaaaga atacaataaa taggttttcc caccaccgat tgcaccagca
    15541 atcaacatat ttggtgattg atcatatacc caatccaaag ttttcattaa atgaatacgt
    15601 ccattttctg ctatacattg atcaatagga atccgaccag aatctgaaag gaatacataa
    15661 ttgattcggg ctttttctcg ttcttcacga attaaatcac gcaaaaatac ttcagataac
    15721 aaatgaccca tttttaaaaa tcgttcgtga aaacgcccac catctaaagg aaaagaaacc
    15781 actaattctt gtttcttttg ataaaaataa acttttggta atctcatttt ttcccttgtt
    15841 tgtttctcac taacttgttt ctttgtataa acaaaccgat tgtcaattaa cattcgaaca
    15901 agtaactgac gccaataaac agaggcaaaa tatccttctt ttagtcgcga ccaatgcatc
    15961 aaccctaaaa ttaacccact aaccagcaac aaatacaaca caatacttat tccaggaata
    16021 agatacattt gccaattcga aaacgtactt aaacgtggat aaaatacttt ccaactaaac
    16081 caacctaaca gaatcaaaaa tggaaacaaa aagaaaccat aatagcaaaa ccataaataa
    16141 cgattgaaat agcgaattcg ccgaccacga tacttgaaaa aatgagcttt caattgtcac
    16201 attcctcctt tctgttttta cttttcctgt gctaaaaact tttgacaaaa agcaactaat
    16261 acaggaatat cacatttagt aaaaaaacat aatcgaaaaa gatttaatcg atttttataa
    16321 gggtgtgcac acatattaca tattcgtact atacgaatcc aaatcacttc ctgatgatct
    16381 tgtttccact ggtacggtaa tgaaaaacga acaagatcac gtaaaaacat acactgccca
    16441 ataaccgcac aaatatatgc atttgtttgt acaaaaatcg ttggattgtc tccaaaatac
    16501 tcaatcaatt gttggatatt ttgataacaa gcacaatctc tcaaagtatt tgattggttt
    16561 tatctgaatt caaaatctcc ccttccttct atcttagata aaaagaatag gaagactcct
    16621 tcctattctt tcaaaaaaac cttatttttt ctctttttcc ttagaatcaa cacttggttg
    16681 tttttgatct aaaccttttt cttgtgattg tttcatatta ggattatttg ttggagcttc
    16741 tactttctct aatcctttcg cataaatcac taaatctgaa tattcacgat tgccatcttg
    16801 tgctggaata tataccacat gatattctgg atcaattaat ttcacaggat cacgtcgttt
    16861 aaaaccagtc aaatctacat ctgctggaaa tttaactcgt cgtgcaatct ttggttcatc
    16921 tggatttcca aaaactaatt gtgctcgctg tgatcgaatt tctcccgtat aatcgcctgt
    16981 ttcattatca aatacacgat cagactcttc taatgcgcca aagcgtacgg ctcctaaaca
    17041 agtttcatct aatgcttctg taatgtcata attatttgga aattttaatc ccatcttaca
    17101 aaacctcccc aatttttaca ccagttaaaa agacaccaac atcttgatag ccagttgttc
    17161 gacgctctgt ttgtcccata tcatttgttt cacgactatt cactcgttct ttacgaaaaa
    17221 caaaataagc ttcttcaaat gaaatacgca cctctttttc tggtaatcgt aatcttttat
    17281 cctcttcagc tgtgagataa acaaaaaaag caggctgttc ttctcgcgaa cactctgctt
    17341 gtaattgaac atatcgccca gacacaccat taatttcctt tggtggatta ggttcccgtg
    17401 aacccaataa aaatacttga cctaaaaact ctttcttcat acgcaatgct ttttctgcca
    17461 taaagttttt cctcctctta aaaaccaaaa taaattctaa atatttctac attacaatca
    17521 aacaagcggt taaaaatcct aaccgcttgt tcctcatgac taatctttgg ctttacgtac
    17581 tttataaagt gttataccta aagcaatgac taatactact aatccaagaa ccataagtat
    17641 actacttgtt tgttcacctg tttttggcaa cattggtaca acagtagctg gtttaacccg
    17701 ttgttctggt ttcactggtt gcggtggttt tggcgcttcc acaatttgaa ccgtttgatc
    17761 tttgtttgtt agatctacat tgtgtttcgc cacttcttca aacgtttctt tctcatcttc
    17821 cgttttcgct tggagccatt ccgcaaaact caagcctttt cctgcaagat tgcgaccgtc
    17881 cactaagaat tcaaataaga attccatcat ttctttatcg gccgcaaaga ctttttccgc
    17941 atgagtgcct tcaaatggtg tgccgtctgg tgttaagtag ccttgaacaa ttaagcgata
    18001 gatttgacca atcgcaagac cgtctacttt gacccattca agcacagttt gatttggcaa
    18061 ggccaagatc tcttttccgc catttttatt cgtcgctttc gtcgtaatgg ttggattact
    18121 gattttgatg gtttggtcat tgtcggtaat atctgcgtga gaagccaata atacgccttc
    18181 tctaaacaga tcttcaaaga ccacgacttc tttgcctcgc aacgcacgtg cgtctagaat
    18241 aaagtctaga ttgactgtac catccgttgt ttctgccaca aacgttaagg tacttgtgac
    18301 ttcttttcca tctactagca ctggtttttc cgttactttg tccattagaa caccgttgac
    18361 tgtgtatgtt tttccaacga ccaaatcgtg gtatttcaca gcgtctgtaa tggtgacttt
    18421 cgttaatgga ttaacctctt tcaagccagt gggtttatgt gtagccgttg tacgtacact
    18481 tgggttagtt actcgtaccg tttgcccttt gtcgttgatg tctttatggt ttgctagttc
    18541 ttcatcattt ctaaaaagac tttcaaacac gacgatttca cgaccattta tacctgtcaa
    18601 atcaaaggta aatggtacga tcaccgtttc attttctgct ttggcaataa agccaacact
    18661 cgtttctacc gtatttccat gcacaagcaa gggttcaccg gttgctttat ccattaagat
    18721 ccctttgacc aagtatgctt ttcctggaag aatctcggta atttcaacgg tatcgtttaa
    18781 ggtaaacaat cctgttgggt ctgcgactgt caatccgtct tcaaagacag ccgtggtatg
    18841 aacggttgga ttattcactt tcacactttg ggcttcatcg ttgatatccg catgtgctgc
    18901 aacttcaatg ccatcacgga aaagtttttc aaaaacaacg atcgtttgac ctttcagaac
    18961 actagcatta accacaaatt caaggtcttt tgtgccattg actttgtctg caataaaggc
    19021 caaggtactt gtgatttctt tgccatcttg taaaattggc gcattcgttg ccttgttcat
    19081 caatactcct gaaatggtgt attcttttcc gacaaacaag ttggtataag aaacactgtc
    19141 tttaatgacg atcgtttcta atggatcaac cactttttca ccatcggctt tatttgacgc
    19201 ttgtgtttct agttttggtg gaatcaagta attacgtaca gaaaccacta acgtttcccc
    19261 atcatctggc gcattttgtt tgaatgcaac cgtgaatgct tgtttatctg tatttggttg
    19321 gaaactttct aatggggcat tttccaagaa gtaatagtca tcggcaacta gacctttcac
    19381 taaaatttcc ccgttttgat cagtggtata ttcaccaatt gcttgatttt taccgttata
    19441 caaggtaaag ttcacacctg cgagcggcgt tttcttcttg ttccattctt catacttgaa
    19501 taattttaca cttttccgtg cttgacggtt cgcaaaatgg atcactttga tcggttctgt
    19561 atcattggta atcgagaaca ctaatgggtt ttgattcaaa acgtaattta cgggggcttt
    19621 gatttcttcc aaacgatagc gatcttttcc ataagtaagt gtaccgttag ttaggaaaaa
    19681 gcctttttca ttggttttaa agatatccgt tgtttctgta tcatttggaa tctccattgt
    19741 gacataacga ttggctaaag tatcaaaaat cttgaattct gtattagcaa gtggaatcac
    19801 ttttcctgtt tcttgatcca ctttcaccac tttaagacgt tgttcaatta ctttatcttc
    19861 taaaacataa tggaaagttt gcccattttc agaaattgtg acttcaagtg gttcaaatag
    19921 tttataccct tcttttcctt tggtttcaga aagaatgtac gtatcaaaga caacggtatt
    19981 tttaccaaaa gtagcataac catcattgtc tgtcacaacg gttttcacca ctttgccagt
    20041 tgtttttgac gtcaaagtga tttcaatgcc agctaatttt ggtttgttat ctggattcgt
    20101 tgaatcgact aaatctttat tagcaacttt cactaaatca atggttcctt tgatcacttg
    20161 atcttttgcg gtcgcattga cagtttttcc aggttcaaca acaatttcaa ttgcttcatc
    20221 agaaagcaca taaccttttg gcgctttgat ttcttttaaa acatatttgc caggatcaag
    20281 atctttaatt tctgcctgta ctttgccgtt aatttttttc aaggtaattt ctttaatctt
    20341 tttcccatct gcttgataca aaccatagac cgcattttct aaagaagctg caccttgtgc
    20401 tttcacacca gtttccgcat cttctttaaa cacacgaatg tttccttgtt ttagaatttg
    20461 taaattaata acagacgcat taggttctgt tggatgtaac acaccaattg tttgagctcc
    20521 tggacgacga taggctaatg gcgcaccttg aactttaatt ttattcaatt gaatttttgc
    20581 tttatccggt gcctcttttg tgactgtaat ccatacttca ttgccgtgaa tagatacatt
    20641 gacacctttt ggtttttgaa tgtctttcac atatttcaaa aaatcatttt tattctttac
    20701 tttcaacgtt tcgccaattt ttaccgtatg tgtttgatta ttaaaagttg ctttttcacc
    20761 aaagttatta atcaaacgtt gaatttctgc ttttcgacga tcataatcaa cacggatatt
    20821 tttgattggg atttttgtgc ccaaagcttc ccaaatcata aattgtgccg caatgtattg
    20881 ttcatcggat ttgtctttat ttgtgacata tcctaaataa gagatcaagc ttgttttttt
    20941 ccgtacattt gaatttttca aatagttttt caaagcagta gctgtataaa cttgaccttc
    21001 tgcggctgag acaccaggct caatacaaaa cgccacttgt ccattaatct caaaacgaac
    21061 catataattt aaagtgaagt ttagattgtt cggactagaa gtaacaccaa tattttcgcc
    21121 ccaatcaatt ttccgaccac ctaaaacagc tgcattggtt gcacggcgaa cacgaaccga
    21181 acgattggtt ggtactgtta ctaacagtgt aggtgtacct gtttttggaa tgttctgctc
    21241 ttttttcttt tgattttcaa tactagccgt agattctttg tttggtttcg ttggttgatt
    21301 ttgactagct gcggttgaag agattgtttt ttcaacgttt tcctcatttt ttccttcttt
    21361 gtcagcagta gccgctggtt gttccactgt ttcttctatt cgtttctttt cttccgcttg
    21421 tttctcagaa gactctggct gcacagaagg cgttgcttcg gcagcttttt ctccttctag
    21481 agtggacgtt tctgaagcag gggttgtaat tgaggcagtt tcttcaactg gttgtgaagt
    21541 accaggacta ttccctgggt gttgtgtttc cccaatcgtc tgatcgagag tcgttttgga
    21601 attgaccgtt ggcgcttctg tcacaggggc ctgttcaaca gtggcttcac cagctgggac
    21661 cccggcttct gctggttgga cttctgtcac ttcagaagcg acttgggcaa tggcttccat
    21721 cgtaggttgt atagccgtcg tttcttctgc atgtgcttga gcagttccca ccaagaaaca
    21781 gaattgagaa aaagccacca atgcgagtgc tttcaaccaa ttcattttcg tttttgcata
    21841 cacaagaatt cctccttttc ctgagaattt tatataataa aacaaaacgc cgttaccaaa
    21901 agtgataacg acgtgaactg tctcttctat ttttatctgg cgctcttatc tgaacacatt
    21961 tcatcaatga ttttaccctc ccactataat tacagaacaa aaaaagaacc tgcttcgtgg
    22021 aaatcaactg tccatttccc aattggaata ccagcttttt tggcaacttc accatcacca
    22081 aatctcatgc cataccaatt actaccttca tccttaattt ctttccaacc atatgacata
    22141 gcttcatcta atgaatcaaa aatcatgaca ctattgccaa ttgttacagg atctggctta
    22201 agatcttctg gaagagaaat atcaatcata ccataatgat ccgattcctc agtattattg
    22261 tcattactag ctactggttt ggttgttcta ctggcttatt ttgatacaac aagtactctt
    22321 attcttcttt tacttctccg ttttcaattt cccattcaaa ccactccgca aagtcttgct
    22381 taaaaccatc tactcgatac ctattttgta aataggcatc ttctgacgtc gtaggatcta
    22441 aataggtagg tttattctct ggacgagcat agacacctac ccatgtttcg taatctcctt
    22501 caaaataact atctaccaaa tagccttttt ttctaagaat ctcccgaact tgatttttaa
    22561 ttattgatac ttgttcaaaa ccatcactca ttctatcacc tcaaataact ctagattctc
    22621 ataaatattc ccgacaattt caatcctatc aatgatctct aacaaatcgc tacttatgct
    22681 tccccattcg tataagaatt tcccttccgc aaaataaatt ttccataaac ttcttcatga
    22741 tcgtcccaac caatatcact ctcgaaaaat tccacgttgt tcttatcttt tagctcagta
    22801 aatgccataa gttcaacatg ttttccaacc tcgaaacaat cacaccaccc atcaaccgtt
    22861 acacgtgtgc cttcaatacc taagaaactt tgtcctcgtg tattaaacac taaaactttc
    22921 cgcatttttt gctcattaat taatcacgcg cgaaatctag gaatcacaac ttattcgctt
    22981 cttctttata aataaaaaaa ctccaataat cggaaattac tatcttttcc tcattccttt
    23041 tttaaatctt catacttctt cttatctttg tcctaaccta ataacacaaa ttttgaagaa
    23101 aaactcagta aaacctgatt ctttttttat cttcacaaca accaactttt tcttcagatc
    23161 cttttactag aacttgtata caaaaattgt tgaatgctga tataatttgc ggctacttga
    23221 aaaaacgata gatgtaaaaa gataaataga atcatttttt attctatatc agtctatgat
    23281 tcattgctta tcaatcaccc aacccattcg atgccgtttt ttttagagaa atttaaaaaa
    23341 gaccgtacga atctcgcatt atccgattac ttttccagaa aagatcccca attctgtata
    23401 aataacccta tcttcttgtt ggtttcattt tctcatataa gaattcagtc aatctaccca
    23461 ctatgaccat tactacgtcc aacacaactg acatattaac cacgtaagta ttccctcaca
    23521 gatctccgaa tctccatggt atggattccc atcctgacgc tgcagaggac aacatatttc
    23581 atgtggctct gtaccctttt gatttggaaa cactacaaat caatactcat ctataactta
    23641 tcaagcgaac tttcgctagg cttctagtga ttactaaatt gttggataac atacatagcc
    23701 cattttatct agctgttatt cataacagtt agttcttgta ttacttcttc cgcttgtgtt
    23761 gccattgagc ttgtcaaaaa gttacttaga aaaaagctgc cagtaaagca actttcaaca
    23821 aacccttttt gtttcttatc atatcaagaa acctcctttt ataaattttt tctataataa
    23881 aaaacgtcga taacttaata agtatcgacg taaactattt ttatatcccg taaatcaaaa
    23941 attctaaaga aagatttcct atataagcat attggataat tcgattagta ttgggtatat
    24001 ctctaccatt ctcataccta tctatggacc attttgaaat tgaaggagaa aaaagctttc
    24061 caaactcctc cctttctaga taataatctt tcctaatttt cctcatcctt aatcccatac
    24121 taaaagaatt tatcttttta aaatcgctaa ctctttcatc ttttggaagt atattcttat
    24181 aatcttctcc gtacataagg taaggaacag tgaccttacc aagataggca atattcatta
    24241 gtttttcaat atctggaata ttaatacctt tttcccatct acttataaca tttttgcctg
    24301 tatagatggt aaagttttta ccaaattttt caagcgtctc ttcccggttc tttcggactt
    24361 ctcgtattct ttttcccata acaattttat cgtcttttga taaactaaat atctcactat
    24421 cacgattaat ctccttcaaa atttcttttt cattacttaa tagaaaaata tcgatttttt
    24481 ttgcatttct tttgacggat ttctctttct atttaacaca taactgagta atacttcact
    24541 aattcctata ttatcagcta actctttgtt aagtaactta ttcatatctc tgtatgcaat
    24601 tagtttaccg atgagaatct caatcttatc ttcctcatct tttattagag tttctatatt
    24661 cagaatcttg cttagagtat tatctacgtc tttagggatt tttcttgacc ctttttcata
    24721 tttaactaaa gtactttgtg aaatacctgc taaatcagct aacttttttt gagatagttt
    24781 tttctctttt cttagagatt taagaaccct tccacttgaa ttatcaacca tgaaaaaagc
    24841 acctcctatg agatgctttc atcatatact gacagctaaa aatcttcaag aaaaatattc
    24901 caattttgtt aattttagta tcgccgtgat aaccaaatga cagtttaaaa tcttttatac
    24961 agataatcct cagtgttgca tctactcaaa aaggtttatt taataggttt ttcgattgat
    25021 tataagttat ttcacactat cttgctcatc cattttcaga acggccatga aggcttcttg
    25081 cggaacttcg acggagccga tttgtttcat ccgcttcttc ccttcttttt gtttctctaa
    25141 gagtttacgt ttacgagaaa catctccacc ataacattta gccaagacgt ttttacgcaa
    25201 ggctttgatg tctgaacggg caacgatttt ttgtccaata gctgcttgga ttggcacctc
    25261 aaattgttgg cgtgggatta gttttttcag tttttcaacg attgcttttc cacgttcata
    25321 ggcaaagtct cggtgaacga taaagcttaa tgcatcgact ttttctccat tcagcaagat
    25381 gtccatcttc actaacttgc ttttttgata accagacatt tcataatcca atgaggcata
    25441 gcctttcgtg cttgatttca atttgtcgaa aaagtcaaag acaatttcag aaagcggaat
    25501 attatagacg acatttactc ggtaatcatc taaataatcc atcgtaatga attctccccg
    25561 ttttcgttga gaaagttcca ttactgcacc gacaaaatca tttgggacca tgatctgtgc
    25621 tttgacgaat ggttcttcta catcctgaat cgttacaggt tcaggaaaat ctgccgggtt
    25681 atctactgta gcagtcgtac catccgtttt attaacatgg taaataacgg atggtgcagt
    25741 cgtgatcaat tctagattga attcccgttc caagcgttct tggacgacat ccatatgaag
    25801 aagtccgagg aatccacagc ggaacccaaa acctaatgct tgagaggttt ctggttcgaa
    25861 ttgtaaagca gcatcattca attgcagttt ttctaaagct tcccgcaaat cgttgtaacg
    25921 agaagtatca attggataaa gaccacaata aaccatcgga ttcatttttc ggtaacctgg
    25981 taaagcttca gcggcaggat tgtctgctaa cgttactgta tcccctactc gtgtatcttg
    26041 gacagttttg atacttgctg tgatataacc gacatcaccg accatcaaaa agtctctagc
    26101 aactgctttt ggcgagaaga ccccgacctc tgtaacatca aatgttttgc cattactcat
    26161 cagtttgatt ttgtctccgg gtttgaccat accgtccgtg atccggacat ttagaacaac
    26221 accgcggtaa ctatcataaa tagagtcaaa gatcaaagct tttagcggtg catctaagtc
    26281 tccacttggt gcaggaacat attccacgat ctgctcgaga atatcttcaa tcccaatccc
    26341 tgatttggca ctggctaata cagcttcact agcatcgatt ccgattacgt cttcgatttc
    26401 ttgacggaca cgttcgggat ctgctgcggg taggtcgatt ttattgatga ctggcaggat
    26461 ctccaaatca ttgtctaacg ctaaataaac attggccaat gtttgtgctt cgattccttg
    26521 tgctgcatcg accactaaaa cggcaccctc acaggcagca aggcttcgag agacttcata
    26581 ggtgaagtcg acgtgccctg gggtgtcaat caagtgaaag atatatgttt ctccattttt
    26641 cgctgtgtaa ttgagttcta cagcatttaa tttaatcgtg attccacgtt cacgttccaa
    26701 atccatcgag tctaacagct gatcttgcat ttcgcgagac gtcacagtgt gcgtcatttc
    26761 taaaatacga tcggccaatg tagatttccc atggtcgata tgagcgatga tcgaaaaatt
    26821 acgaatctgc tcttggcgtt ctttcatttt ctttatatcc atagcgtctc ctcttttcat
    26881 ataatcagca ctttccatta taccaacgaa aagcagtaat ttaaagagtt tttaattagt
    26941 cagctataga agtttttttt gtagaatttt gctatactgt tatcaagcta ataaatggag
    27001 atgaaaaaat ggatcaaaaa gattttagag aaaatcttga attaaaaccg gtcactcaag
    27061 aacatatcga tcaattcaat gaattgcttt cctatgtgtt ccaagtgact gaagcagaca
    27121 tcgaggaaag cgggtttgag aataaacgag cttttatccg ttccaaaaaa ccgattttgg
    27181 aagtatcgaa agtgttcgga tggttccacg aaaataaact gatctctcaa atcgctatct
    27241 atccttgtga agtcaatatt cacggtgcac gttataaaat gggtggtgtg actggagttg
    27301 gaacttatcc agaatatgct aaccatggat tgatgcaaga tctaattatc gttgcattgg
    27361 ataatatgcg caagaataag caatggatct cttatttgta tccttacagt atcccctact
    27421 atcggcgtaa aggatgggaa atcatgtctg ataaactttc ctttaaaatc agagatacac
    27481 agctacctaa acaaatccca gtaagtggta tggttgagcg attaccagtt gatcatccag
    27541 acgtattttc cgtttatgac aaatttgccc aacaaaacca tggtgcttta ttccgcagtg
    27601 attttcattg ggaagagtat tggcgtttcg aaaatgaaga tgagcgcaca gccgctgtct
    27661 actacgatag caaccatgaa ccaagaggtg ttctgttcta ctgggtagct gaagaaatat
    27721 ttcacgtaaa agaaatgttc tatcttgatc aagaagctcg aaatggctta tggaacttta
    27781 tttccgcaca tttttccatg atctactggg tacatggaga tatttataaa aatgagcctt
    27841 tagcattttt gattgaagat agtcagatca aagagcagat cgaaccatat tttatggctc
    27901 ggatcgttga tgtaaaagaa ttccttcaac ggtttccgtt tgttggaaca gctgatgctt
    27961 ttcatttcat cattgaagat cctgtcgctc cttggaataa tggtgtcttc gctttaacat
    28021 gggatgaaca gggacaagtc cgtgtcttaa atgaacctat aggaaaatcc gttcgactgg
    28081 atatccaaac attgacttgc ttgatgatga attatcgccg tgcttcttat ttggcaagaa
    28141 tcgaacggtt agaaacagat gaagaaacac tgaagtcact agagcgtatc attccaaaca
    28201 tggaagctta ctttagcgat tatttttgat aaaaaggatg atgtttagat gaaaatttat
    28261 tttgccgcac caatgtttgc taaaagtgat ttgttatata accaacagtt agttaaagaa
    28321 atcagagagc tctcttctga gctttcgatc tatcttcctc aagaaaatga agcaatcaat
    28381 gacaaaactg cttatgcaga cagtcgaatg attgctcttg ctgacacaga aaaagtgttg
    28441 gctagtgatt taatgattgc tttactagat gggctgacga tcgatgccgg ggtagcatcg
    28501 gaaatcggtg tggcttatgc gaaaggcatc ccagtgatcg gcttatatac tgatactcga
    28561 cagcaaggcg gaacccaccc gaaaaaaatc gctgcactac aagaaacagc tgaaaatcaa
    28621 tttcactacc tgaacttata tacaataggt ttgatcaaat tgaacggaaa agtggtttct
    28681 tcagaaattg agttgctttc agaagtaaaa agatttttag atggagggac tttcagtgat
    28741 taaagaaatc aaaaaggtgc aaatagcttt acttgctttt ggtgtattcg ttgtctgcta
    28801 taatttttat gaatttatca cacaaaaata ttcgacttct caaggaatca catttatcgt
    28861 ggaatcttta ttagggattg cattgatttt catgccacaa gtcattttga aagttttcaa
    28921 acttaaaata ccagcagcaa tcgttttgtt ttactggttt ttcttgttca tctctgtctt
    28981 tttaggcaca gggatgcatt tgatcagtat catctctttt tgggacaaaa tcttacatgc
    29041 ggtgagtcca atggtcctga cagctcttgg ctatggattg attggttact tgatgaaaga
    29101 tgctgagata tcaaaaacca gtccttggtt gttcctctta ttcggctttg cgtttgctgg
    29161 actttgcggc gtgttttggg agttctggga attcttatgc gaccagttct taggcatgaa
    29221 tcttcaacgt tttgctgctt ctgatggcac actgtttgtc ggacgagcag cattgatgga
    29281 tacgatgggc gacttgctga caaatacgat aggtgctgcc ttaatgggat tgtttgcatg
    29341 gtctcaaagt aaaaaagatg agcgttactt tgaaagttat aaattagaaa aagtgaaaaa
    29401 tacctaataa aagaggttgt gacagaagcg ggcagcttca agaaatttcg gcttattttt
    29461 cgaagaaact acttctggaa cactgtttat ttgaaaaaag gaggtctgac actagttgtg
    29521 tcagacccct tttttctttt atagtctcaa tggaagatta gacgatttct tctagctctt
    29581 ctgttttgaa atgaagttgt ccatcaagta aagtaatcgt tacttttgat ttaggcataa
    29641 catgtcctgc aacgatttct ttagctagtg gtgtttctac ttccttagtg ataaatcgtt
    29701 tcaatggtct tgcaccatat gctggttcat aagcattttc agcaatccac gttttggctt
    29761 catcactgat tgtcagcagg atttcttgat gttccagacg ttgtgccaat tgcgccacca
    29821 ttttgtcaac gattcctttc acgttgtcta ggctcaatgg tgtaaataaa atcgtgtcgt
    29881 cgattcggtt caagaattct ggtttgaagt ttccacgtaa tagcgtgttg acttgttcag
    29941 ccactgcttc tggaatcgtt ccatcagcag tcacgccttc cagcagtaac tgtgaaccga
    30001 tattgctggt catgatcaga accgtatttt tgaaatctac tactcgccct tttgaatcag
    30061 tcaagcgtcc gtcatctagg acttgtaaca agatattgaa gacatcggga tgcgcttttt
    30121 cgatttcatc caacaagacg attgtataag gatttcgtct gacagcttcc gttaattgac
    30181 caccttcttc gtaacctaca tagcctggag gagctccgac tagacgagac acagcatgtt
    30241 tttccatata ttcactcata tcgatccgta ccatatgatc ttctgagtca aacaagttct
    30301 cagctaacgc tttcgctaat tcggtttttc cgacaccagt tggtcctagg aaaaggaaag
    30361 aacctagagg acgatttggg tcttgcaatc cagcccgtga acgaattacc gcgtcactga
    30421 ccgcatcaac tgcttcatct tgaccaatca ctcgtttatg aagtgtttca ttcaatttaa
    30481 ttagtttttc tcgttcacct tctacaagtt tcgttacggg gatacccgtt aatctgccaa
    30541 cgacttgagc aatctcattt tcggtaacgg actcttgaac catcttaatc tcgctgtctt
    30601 ttgctttagc ttctaattcc ttcaattctt tttctaattg aggtatcgta ccatgacgca
    30661 atacagcagc gcgttctaaa tcatagttat tttctgcatc ttctaattcg tgttttgctt
    30721 tatcgatttc agcacgtttt gctgaaacag aattgacttc ttctttttct gtttcccact
    30781 gcatcttcat cgcatttgct tcttcacgaa gttcagctaa ttcttcttgc aaatttttca
    30841 agcgtttttt acttgcatca tctgattctt ttttcagtgc agcttcttcg atttccaact
    30901 gcatcaaacg tctagtaact tgatctaatt ccgttggcat agagttcatt tctacccgaa
    30961 tcgttgcact ggcttcatcg atcaaatcga ttgctttgtc cggtaagaac cgatcagtaa
    31021 tataacggtc agataaagta gctgctgcta ctaatgcgtt gtcatggatg ttaacaccat
    31081 ggtggatctc aaaacgttct tttaatccac gcagaatact gatcgtatct tccactgttg
    31141 gttctttgac taataccttt tgaaagcgac gttccaacgc tttgtctttt tccatatatt
    31201 gacggtactc atctaaagtt gtggcaccga tcaaatgtaa ttcaccgcga gcaagcattg
    31261 gtttcaacag atttccggca tccatacttc cttctgtttt tcctgcccct acgatattgt
    31321 ggatttcatc gatgaacaag aggatacgtc catcactttt tttgacttct ttcaagactg
    31381 cttttagtct ttcttcaaat tcaccacgga attttgctcc agcgatcaat gcacccatat
    31441 ctaatgaaaa aatcgtttta tcttttaaat tttcaggcac atctttgcgt acgatacgtt
    31501 gagcaagccc ttcaacgatt gccgttttcc ctacaccggg ttctcctatc agaacaggat
    31561 tattttttgt ttttcgtgaa agaatacgga tgacatcacg gatttcttca tcacgaccga
    31621 tgattggatc catttttccg cttttgactt gctgtactaa atcaacgccg tatttttcta
    31681 atgctttgta ttgttcttct tggttttgtg aggtcactct ttctcctcct ctcatctctt
    31741 cgatgttttt acgcaattct ttttcagata atccgttttt attcaaataa acagttaacg
    31801 gataattttt taatttcatc aacgctaaaa taacgacttc cgttgaaaga aactcgtcgc
    31861 cgaagctttc acgtatttgg tctgcttcat tcaataatcg aaagaaattc tggctgaggt
    31921 tttgtccata ttggacgttt ccaccagaaa caactggata ctcatccagt agacgatcaa
    31981 tttcatgttc aaaagattcg acatccaatc cggcatccgt atagaaattt cgaccaaaat
    32041 gattagattg taaaaagatt ttccatacat gggcaatgtc gatatcttga tgttttcgag
    32101 tgaccgcaat ctgttgagct tcagcaatcg cttcttgaag cgtcgtagtc attttctcaa
    32161 tattcataca ggtataccct cctaattaaa ggtaagatca cttatttctt tgatctttac
    32221 aaaaactatt atatcgcttt ggtcaatttt ggtcaaaata tacacatagt t
//
LOCUS       DAYYGO010000018        31203 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000018 DAYYGO010000000
VERSION     DAYYGO010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.018"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(101..1309)
                     /locus_tag="ACHA5T_000733"
     CDS             complement(101..1309)
                     /locus_tag="ACHA5T_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8886331.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(1328..1606)
                     /locus_tag="ACHA5T_000734"
     CDS             complement(1328..1606)
                     /locus_tag="ACHA5T_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886332.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            1799..2455
                     /locus_tag="ACHA5T_000735"
     CDS             1799..2455
                     /locus_tag="ACHA5T_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM8886333.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(2897..3694)
                     /locus_tag="ACHA5T_000736"
     CDS             complement(2897..3694)
                     /locus_tag="ACHA5T_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFM8886334.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(3691..4875)
                     /locus_tag="ACHA5T_000737"
     CDS             complement(3691..4875)
                     /locus_tag="ACHA5T_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFM8886335.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(4900..6147)
                     /locus_tag="ACHA5T_000738"
     CDS             complement(4900..6147)
                     /locus_tag="ACHA5T_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFM8886336.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            6362..8107
                     /gene="dnaX"
                     /locus_tag="ACHA5T_000739"
     CDS             6362..8107
                     /gene="dnaX"
                     /locus_tag="ACHA5T_000739"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFM8886337.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            8127..8441
                     /locus_tag="ACHA5T_000740"
     CDS             8127..8441
                     /locus_tag="ACHA5T_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFM8886338.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            8466..9062
                     /gene="recR"
                     /locus_tag="ACHA5T_000741"
     CDS             8466..9062
                     /gene="recR"
                     /locus_tag="ACHA5T_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFM8886339.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            9126..9770
                     /gene="tmk"
                     /locus_tag="ACHA5T_000742"
     CDS             9126..9770
                     /gene="tmk"
                     /locus_tag="ACHA5T_000742"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFM8886340.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            9785..10114
                     /locus_tag="ACHA5T_000743"
     CDS             9785..10114
                     /locus_tag="ACHA5T_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFM8886341.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            10128..11066
                     /gene="holB"
                     /locus_tag="ACHA5T_000744"
     CDS             10128..11066
                     /gene="holB"
                     /locus_tag="ACHA5T_000744"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFM8886342.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            11102..11926
                     /locus_tag="ACHA5T_000745"
     CDS             11102..11926
                     /locus_tag="ACHA5T_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFM8886343.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            11919..12266
                     /locus_tag="ACHA5T_000746"
     CDS             11919..12266
                     /locus_tag="ACHA5T_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFM8886344.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            12335..13207
                     /gene="rsmI"
                     /locus_tag="ACHA5T_000747"
     CDS             12335..13207
                     /gene="rsmI"
                     /locus_tag="ACHA5T_000747"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFM8886345.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            13316..14437
                     /gene="dinB"
                     /locus_tag="ACHA5T_000748"
     CDS             13316..14437
                     /gene="dinB"
                     /locus_tag="ACHA5T_000748"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFM8886346.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(14491..15093)
                     /gene="nrdG"
                     /locus_tag="ACHA5T_000749"
     CDS             complement(14491..15093)
                     /gene="nrdG"
                     /locus_tag="ACHA5T_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFM8886347.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(15408..17597)
                     /gene="nrdD"
                     /locus_tag="ACHA5T_000750"
     CDS             complement(15408..17597)
                     /gene="nrdD"
                     /locus_tag="ACHA5T_000750"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFM8886348.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            17923..18690
                     /locus_tag="ACHA5T_000751"
     CDS             17923..18690
                     /locus_tag="ACHA5T_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8886349.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            18680..20722
                     /locus_tag="ACHA5T_000752"
     CDS             18680..20722
                     /locus_tag="ACHA5T_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8886350.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            21003..21152
                     /locus_tag="ACHA5T_000753"
     CDS             21003..21152
                     /locus_tag="ACHA5T_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFM8886351.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            21163..22677
                     /gene="dltA"
                     /locus_tag="ACHA5T_000754"
     CDS             21163..22677
                     /gene="dltA"
                     /locus_tag="ACHA5T_000754"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFM8886352.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            22674..23879
                     /gene="dltB"
                     /locus_tag="ACHA5T_000755"
     CDS             22674..23879
                     /gene="dltB"
                     /locus_tag="ACHA5T_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFM8886353.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            23924..24157
                     /gene="dltC"
                     /locus_tag="ACHA5T_000756"
     CDS             23924..24157
                     /gene="dltC"
                     /locus_tag="ACHA5T_000756"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFM8886354.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            24160..25428
                     /gene="dltD"
                     /locus_tag="ACHA5T_000757"
     CDS             24160..25428
                     /gene="dltD"
                     /locus_tag="ACHA5T_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFM8886355.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            25569..26651
                     /locus_tag="ACHA5T_000758"
     CDS             25569..26651
                     /locus_tag="ACHA5T_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFM8886356.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            26651..27454
                     /locus_tag="ACHA5T_000759"
     CDS             26651..27454
                     /locus_tag="ACHA5T_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFM8886357.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            27524..28264
                     /locus_tag="ACHA5T_000760"
     CDS             27524..28264
                     /locus_tag="ACHA5T_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8886358.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            28275..29279
                     /locus_tag="ACHA5T_000761"
     CDS             28275..29279
                     /locus_tag="ACHA5T_000761"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFM8886359.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(29324..31168)
                     /locus_tag="ACHA5T_000762"
     CDS             complement(29324..31168)
                     /locus_tag="ACHA5T_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886360.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         9934 a         5499 c         6344 g         9426 t
ORIGIN      
        1 aatagatgtt taattaagaa taatcataaa ctatttttac tcaaaaaacc gctcagatta
       61 ttttcagatc tgaacggttt tttttatcta ttcatcaaga tcaaatttca tttttcaatg
      121 attcaatcaa ttcgccctct gcttcatgac tatgttttcg gccattaaca agatagagtg
      181 ctgtacatat caatggaatg actccggcaa tcacatacaa tcctctaaat gaagtaagag
      241 attttagcat ccccatcaaa taaggaccaa cgcctaagcc taggtctaaa ccaataaagt
      301 aggtagataa agcaatactg attcgatgtt cgcggacgat ttttaaacaa acagcctgtc
      361 catttgacat aaacgtacca taacctaggc cgactaatgc tccagagata agcaatgtca
      421 cactattcgt cgttacactc agtaaaaata atcctgctgt taagaataaa tagctaggat
      481 acatcacgta ttgttctcct ttgacatcga aaatccttcc agtcaatgga cgtgtaaatg
      541 tgatgaccaa agcatatacg acgaagaaaa atgacccagc agcaactaag tggattactt
      601 ttgtataaga agataaaaat gataaaacac ttgaataaga aagacccatc aaaaaggcaa
      661 ttcctgtaat gaaaagtgct ttcttttcaa taaaactatt gatattccat gagcgaagag
      721 atattttttg ttcttcagat aatggaatat tttttacagg aaagacaagg caggcaatcg
      781 taacaaagaa gaccagtaca attgaaaacc agataatgaa acgaaaatca gtcatattca
      841 gcaaaatcat tcctaaaaat ggcccaacag cggcagctaa actagtactt aatccataat
      901 aattgatgcc ttcccctttt ttagatgcag ggatatatgc agtaacgata gcatttgtag
      961 ctgtagaaac tgtaccgtag ccgaatccat tcaagaatcg aactaaatac aatgccccga
     1021 tagttggcgt ataaagatat gctaatgttg aaaggagata gaagatcgcg ccgcctctta
     1081 aggtaaactt ccgaccaaat agttctaact gtttccccat gaataagcgt gccagcaatg
     1141 ttccgatgat atagatccca gaagcaaaac ctgcttggct caagctggcg tgtaaggtgt
     1201 cttgcgcaat gactgccatg atgaccatca acagataata aatcaggtag acaataaaat
     1261 tgatcaatgt gattccaata aaatatttat taaataattt cttttccatg tatgttatac
     1321 attcccctta agctgaatat gcttttaatt tttgtatttg aacttctaca tcgtgataat
     1381 tggatggata gcgatcaaca agttggcgaa taaataaatc tcttgtagac ataaacagat
     1441 ttaaaaattc ttcgcattcg tttgttggat tctcatccac tgctttgatc aattcgatcg
     1501 agttttgata cgcatcattt aaaaagacta aagaagcttt catatcaaat cttgtttgat
     1561 atgctgtttc taatcgatta atgattgtgt tcatccagta attcatgttt tttccctcta
     1621 ttcttaaaaa attttacttc tgcagtataa cgaataaaaa aaaggaaaaa aatcgcataa
     1681 gtgatggaaa aaggtactta acaaatgtta atatcattag taaaatacct ttggacttta
     1741 aaaagtttta aagtactgta tgatcttttt agatgaaagt ttggatgggg gagcatcatt
     1801 ggatagtaga gtcttaaagg aatatttgga taaaaatgat tttccgatta tacgcaaagg
     1861 atatcataaa tatttgacat tcgaagggat ggaagattcc catacgtata tcttgaaaga
     1921 gggaacggta aaagcaagtg ttatttcaaa tgatgggaga gaatttaacc ttcgctatat
     1981 caaagatctt gaaatcgtct ctcttttaaa agacgaatat tcacaattca tcgattcgcc
     2041 gtataatatt cgagttgaat cagaatatgc agagttttat cgaatcaatc gtgtgcgctt
     2101 ttggcaaaat atcaaccaag acccaaagct acaacactat gtaaaagagt attaccgcta
     2161 tcgtctgctt tattcgatga aaaaaatgca gcaaatgcta ttaaacggaa agctgggagc
     2221 tgtttgtacg caactttacg aattatatga tttattcggt gtgcggacag aagaagggat
     2281 gctgatcgat ttttcgatca cgaatgaaga aatcgccaaa ttttgtggca tctcaactgc
     2341 tagcagtgta aacagaattt taaaacaact gaaaaaagat ggtattatca ccgttaaaga
     2401 ccatcatatc gttattttga atatggactt gttagaagac aatattatct tttgatgaac
     2461 aaagaggttg tgaaatcagc gttttgacct gagtattaga gaaacaaacg gaaaaatagt
     2521 tcctcatatt ttgccgtttt ttgagctaat accgagggtc agctgattga acaccgttta
     2581 ccaagaggtt gtgagcctgc cgtttaactc tgagtcacaa ggaacaattt caaaaaacag
     2641 cttttcatgt ttgatgaata ttgtgacttg tgaccgagga gttggtagga gaacaccgtt
     2701 taccaagagg ttgtgaaaaa gctgttctgc atcaagtaat aagaagagcc aagtaaaaac
     2761 agtctcttat gtttttaact tggctggact tatcaccgta gatgtaagct tttgaacgcc
     2821 gtttatttgc aacaaaaggg gctgtgacac gatttttgtc acagcccttt ttttgcgaac
     2881 attcatttag caatttttag aaacttctgc cgccaccgcc aaaagtcccg ccaccgctag
     2941 aatgggtagt acttccgcca ccgccgcttc caggaggtgg gcttttgggg attcttcttg
     3001 tcgtgacaaa agaatttgtc agacgatcgg ttttttcagt taatttgata ctggattttt
     3061 ctcggaatgg atatttgtag atgcctgatt tcaattgata acgcgaaaca gtcacaaaat
     3121 aaaagacaag actaaggata agagccaaaa caacagcaat gatgatttct actgtcgtca
     3181 aaaccttata gcgtgtaatt ttgccagttt cttcgtctac acgataatgt cctcctggaa
     3241 ctccgtcttc cacatatgtt gctgatttag acaggtaagc ttttgcagca ttgtaatagg
     3301 cagcatttgt catctgatca taaacatcat ctaagatcga atcgattctg ctatcagtca
     3361 aataatcgat catattacca gaagtcgaaa tgtagatttc acgctgtccc atatctaata
     3421 gcaatactga gccattttga tcatttccga ctgcgtctct taagtaatta tcagcaaagt
     3481 ctcttggttc ttctgaatta gatgtagtcg ttacgatcaa gactcgtcct ttgatttttt
     3541 cattcagctt ttctgcctgt tgttccaaag attggatttg atctgttgtg aataacccag
     3601 cctcatcatt taccgtagct gtatctgcat gagtagccac cggccagcca acacttacta
     3661 gcaaaagaaa gaataaaaaa aattttttca tcatatcaag taacctccga tcagaaagag
     3721 gatcgctagc ccagcgaaaa tcccaagagc ggtcagtccc aattttttct ggctgatcgg
     3781 caggacaccg ctgacctttc ctgtttgtcc attcattgcg tagtagtaga cttttttact
     3841 tgagtcattg cttcgataag taacaagcca taccggcagt aatacgtaat tatttacttc
     3901 gtttgtgatt gaagcagaag ttcgcacacc ggtcaatgtt gtatatccat ttgcggtatc
     3961 ttttaacagc ttctctgaat agtcttttaa ttcagattga atctgtgatt tgattgcctc
     4021 atattcgata tctctttttt ctgcttggaa cccggctaaa tactggcttt taaaatcaat
     4081 tgctttatct aacgggaaag gttgcacgcc ttcgaccatc ttttgttgcg tattttttga
     4141 taaggcattt tttactagtt ctttaaaaga aagcttgcct gctcttgaaa cagcaaattg
     4201 cttcgtttct gtgtattcga tgtcacctat ccgccatatg cggataactg taccattcgc
     4261 ctgcatggca ccatctactt cagcatctgt agcccaataa gggaaataca cacctgtcaa
     4321 cttatcgatt tgatccttat tgaaaaaagc tttgggaaca aaccattttt tcttggtcca
     4381 agcgagaaat ctgttgattg cttcttcttt ctcgacagca aaaggaagta ctttatttgg
     4441 caagaattct ccgcttaaac gtcccgatag tacgacggga ttatggcagt agtaacaata
     4501 agtcgcagca gttgtcgcat cagtcacgat ctctgcacca cagttgggac aaagaaaaag
     4561 atctatcgct gcggtattat ctgcagattc gtccgctgta tctgaaaaac cgcctgcctc
     4621 gtcaaccgct ttgcgctcgg cttcattcat atcggcaagc tgttcttggg ctgtaaaagt
     4681 aaaatcttcg gtaggtgaag gttcttcttt gctttcttcg ccagccatac gcgcttcttt
     4741 ttgcttttgc tcatattggc tgacttcttc ttctgtatag atattcaggc agtattcgca
     4801 atgaaatttt tggtctttgg gatcaaaagt caacggaccg ccgcaattag gacatttatg
     4861 tgtaagaaca tccatcagct gcacaccttt ctaaataaat taattaacgg ggattccttt
     4921 aaacggttta ccacagtttg gacagaattt agggatgccg ttggaaagat ccacgatctc
     4981 attgcattca ctgcatcgca tttgtagctt aggctgaacg ttttctacag gtttggcagc
     5041 tccgcagttg gaacagaatt ttccggaatt ttctgtcccg caaactggac atgtccaagt
     5101 atccgcattt ccttgtgcag tttgttgatt ttgtttttct gcttgttgtt tgatttgttc
     5161 ttggttattt tgagctgctt gagaaagata agaaccattt gcattcattc ccatacctac
     5221 acccataaag ccggtcattg ctcctgcttc attttttccg gctgcttcca taccacgtgc
     5281 aatggaacct tgcacgtagc cttcacgtac gctaggatca cctagcatcg ccccttcgtt
     5341 acgcatattg atcagtttca cagaatcatc ggtgtaagaa atgctggcta cagccacaga
     5401 aacaatctcc atcccacgta attcacgcca agaatcatcc aatgcagtat ccatgtattt
     5461 gcttaattcc aaactcttag atggtacata agaaatccgt tgaccatctg cagacatctg
     5521 attgattgct gattgtagcg ctgttaggaa ttcagctaaa tactgctcat tgatatcatt
     5581 gatttccact tgtgttttgt ttttagggat tgcgtttgta taaaataaaa tcgggtcaac
     5641 gacatgaata gaataagtgc catgtgcccg taagaataat tctgcgttat agaaattgtc
     5701 aaagtagttc aacggcgcgc ttgtaccgaa tttgattccc tttatttctt ggagattgat
     5761 ataaaaaact tgttgtttct gcggtgtgac gccgccaaat ttgaatcggt caaaagtttc
     5821 agcaattgct tctttcagtg ttccattgaa catggaagga gctgcgtcat ttttaatggt
     5881 gtaataacct tcctcagcgg tataatcgat gatttttcca ccatcgacca aaagcatcat
     5941 catgtttgga tagacatgga cgactgttcc gtctgttaaa acatcttctg ttcctttacg
     6001 attggagcca cgtttgtcat ctttgcggac tttgacacct tttgtcatga ccgttgtatc
     6061 actcatattg tctggttcga tgacttctag ccattgatct gccagaccgc cgccaaccat
     6121 acttgttgct gctttgataa gacccataaa aaattcctcc tctgattcag ctagtgccaa
     6181 acaattattg tttatttatt ataccatagg aagatgtgaa acttctcgga ctagagacgg
     6241 aattgaaatg gaaaattgcg tattgtttaa atacctgaaa aagccgagaa ggatagtagt
     6301 ttttttacct aatatcacgt ataataagag atagtgaatt ttttaaaaaa gggggatcct
     6361 tatggcatat caagcacttt atcgagtctg gcgttcgcag cgtttcgaag acatcgttgg
     6421 acaaaaagct gtgacacaga cactaaaaaa tgctattgtt tcccacaaaa cctcacatgc
     6481 ctacttgttt acaggaccca gaggaaccgg aaagacgagt gcagcaaaga ttttcgcaaa
     6541 agcaatcaac tgtccgaaca gtaaagacgg tgagccatgc aacgaatgcg aaatgtgtcg
     6601 atcaattact gctggtacgc aagaagatgt gatcgaaatc gatgctgcca gcaataacgg
     6661 agtcgaagaa atccgattta tccgtgatcg ggcaaattat gcgcctacaa aggcagcgta
     6721 caaagtgtac attattgatg aggtacatat gctttcaaca ggagctttca atgccttgtt
     6781 gaaaacattg gaagagccaa aagaaagtgt catattcatc ttagcaacga ccgaacctca
     6841 caaaatacca gctacgatca tttcacggac tcagcgattt gattttaaac ggatcaatac
     6901 ggcagatatt gtcgaacatc ttgaatttat cttgaaagaa tcgactatca gctatgaacc
     6961 agctgcattg aatgtgattg cccgttctgc agaaggcggg atgcgggatg cattgagtat
     7021 tcttgatcag gcaatctcgt ttagtgaagg aacaattact ttagatgatg ccatgcaagt
     7081 gacaggtagc ttaactgacg aaatgatgga cagttacttg agtgcttgcg tgaaaaaaga
     7141 agtaccagaa gcattggaaa cattgaacca aattctcgca gccggcaaag aatcacgtcg
     7201 ctttctagag gatatgttac aatactgccg tgacttgctg atgtatcagc aagcacctaa
     7261 cctagttgag gaacaatcga gtacgatcag aagtacattt acagaattgg cacaaactgt
     7321 ttcagcggat cagctgtttt caatgataaa gattttgagc gatactcaaa atgaaattcg
     7381 ttttaccaat agtgccgcta tttatttgga agtcgctaca gtcaaacttg cacagctacc
     7441 gtctgcttct gttccttcag cagatgtagc atttgcagat gatcagatag taaaacagct
     7501 acaacaagag attaaggaac taagaaacga aatcaaggaa ctacgcagta atacaggaat
     7561 tgagaataca aaagtacagc cggtacagca aacaaatcat aaaaaacaga caagtaccta
     7621 tcgtgtaccg acggaacgag tatataaagt tctgcgggaa gcgacaagac agcatctgaa
     7681 ccaagtaaaa gaagtatggg aagacttgct gatgagtcta tctgtcactc agcgagcaat
     7741 gcttaaagca agcgaaccag ttgcagctgg acctacagga ctagtgattg cgtttgatta
     7801 tgagattgtt tgtcaaagag cagcaaatga cgaagaattg cagctttcgg tacacaacaa
     7861 cttaagccga atgatcaaag attatgcgcc tgatcctgtt tatattacaa gagaaagctg
     7921 gccagaactt cgaagaaatt atttgacgca ggcacaagaa gagggaacag aaaactcttt
     7981 tgcttctgaa gactcagaag gagaaatcga gttgcttcct caagaacaag aagtaccaga
     8041 agaaacactt gtagtaaaag cagaggaact gtttggctcc gaagctgtta cagtggtaga
     8101 tgattaataa aaaggagtag ataattatga tgcgtggaat gggcaatatg caaggcatga
     8161 tgaaacaagt acaaaaaatg caaaaagaaa tggcagaagc acaagagctg ctaaataaaa
     8221 cagagtttac aggtaaagcc accaatgaat tggtcaaagc ggtttttaca ggcgatcgtc
     8281 gcatgaaaga tatccaaatc aatgaagaag tcgttgatcc tgatgatatc gagatgcttc
     8341 aggatcttgt cgtaatggca gtcaatgatg cgatcgcaca aattgatgtg gaaactgaga
     8401 aaaaattagg gaaatacgca aaaggtttgc caggatttta gttttaaact gaaggagtaa
     8461 tgaatatgca atatccagaa ccaattgcga aactgatcga cagctacatg aaacttccag
     8521 gaatcggtca gaaaacagcg acaagactcg ccttttatac gatcgatatg aaagatgaag
     8581 tagtaaatga atttgctaaa tctttgttaa gtgtaaaacg cgatttgcat ttttgtagta
     8641 tctgcggaaa catcacagaa gaagatccct gcgaaatatg tagaaatcct tcacgtgatc
     8701 gaagcgttat cctagtggtc gaagagccga aagatgtaat ggcaatggaa aaaatgcgag
     8761 aatatcacgg actttatcat gttctccatg gtgtgttatc accgatggaa gggacaggtc
     8821 cagaagacat caatatttct tctctgttgc agcggctgca tgatgcagat gtaaaagaag
     8881 tgattatcgc aacaaatgca acaacagagg gagaagcaac agctatgtat ctctcacgtc
     8941 tgatcaaacc cgcaggtatc aaagtgactc gtctagcaca tggtctgtct gtaggctctg
     9001 atatcgagta tgcagatgaa gttactttat tgaaagctgt cgaaggaaga cgtgagttat
     9061 aaatttctaa ttaagtgttt tttcagatac aatataaaag cagcacaaga gatgggggca
     9121 acaatgtgaa cggactattt attacaatcg aggggcctga tggtgcagga aaaaccagca
     9181 tcttgaatga actttaccct cttttgaaag aagtagcaaa aacagaaatc attcaaacaa
     9241 gagaaccagg aggcattccg attgctgaag agatccgagc agtgatcctt gatccaaaaa
     9301 atgatcggat ggatgaacgt acggaagcac tgctttatgc agcagcaaga agacaacatc
     9361 tagtcgaaaa ggttctgcct gctctggcaa aagggaaaat cgttttatgc gatagatttg
     9421 tcgacagttc tcttgcttat caaggtgcag gccgacgcat cggcgttacc gagattgctc
     9481 ggttgaatga gtttgctaca gaaggtacta cgccggattt taccctttat cttgacgtag
     9541 attcagacac cggattaaga agaatcaaga aaaacagaca aaatcaaatc gaccgtttag
     9601 attcggaagg tttggaattc caccagcgtg tacgtcatgc ttacttaaag ctggcagaag
     9661 agaatccgga aagaatccac aaagttgatg caagaaaaag ctttgaagaa gtgctgcaaa
     9721 caagttatca tacaattatt gaacagtatc cgcaattttt tgaaaactag gaaggtgaac
     9781 gtttatgaaa attattttag cgattatcca agacaaagac agcaaccgtt tatcaaatga
     9841 attgattgat gcaaatatcc gtgccacaaa actatcatcg acaggcggct tccttaaggc
     9901 tggaaacagt acgtttatcg tgggtattga agatgaccgt gtagaagaag cattggaaat
     9961 catcaaaaaa acgtgtgaat caagaaagca atttgtatct acacctgtta cactggatat
    10021 ttcgatggat gggggcgtcc cttatccagt agaagtagaa gtaggcggtg ccacagtctt
    10081 cgtcttacct gttgaaggat tccatcaatt ttaggaggag ctgagctatg aatcaagaaa
    10141 taaacttggc tcaaatgcaa ccgatcgtgt atcagcagct tcaacggagt tttgagcatg
    10201 ggcgtatcgc tcatgcttat ctttttgaag gggagaaagg aacaggaaaa catgaagtag
    10261 gtatatggct tgcacagcac ctgttctgta cccaaatgaa agacaatctg ccttgtggaa
    10321 aatgcaacaa ttgtcagcgg atcgaaaaga aagaacatcc agatgttttg gttattgaac
    10381 cggaaggaca aaccatcaaa gtcgatcaga tcagacgttt gcagacggaa ttcagtcgca
    10441 gcggttacga atcaagaaaa aaagtttttt tgatcaaaga agctgaaaaa atgaatagca
    10501 gtgcggcaaa cagtttgttg aaattcttgg aagagccccc tggagatttt ttagcggtgt
    10561 tagaaacaga tgcagtagga agaattctcc ctactattca atctcgttgt caagttctgc
    10621 attttcaaga actttcgaaa gaagcactga tccatcgatt gcaagaagag cagattccat
    10681 tggaaaaagc aaaacttttg gctttcttaa caaacagttt aggaaaagca gttgaaatct
    10741 ctcaagatga atggtttaat gatgctaagg acttgattta tcaatggttc gtttacttgc
    10801 aaaagcaaga tactcaagcc tttatctatg tccaaaaaaa attggtaaaa tcatttaaag
    10861 aaaaaacaca acagtttaca ggattatctg tcttgatgtt ttattatcag gaagcacagc
    10921 gtcaagcagt agcaaacggc gaatatggca aagtgaaaca gataaaccag ataattgaac
    10981 ggatattgat cgcagagcag aaactacgca gcaatgtcag ttttcaggct gttgcggaac
    11041 agttcgtttt gcatacgatt ttttagcttg ccgcaaagga tgatgatcaa ggaggatgga
    11101 aatggtagaa gtagtaggtg tccgatatag agatgcaggt catatctatt attttgcacc
    11161 aggagactct gaatatgttt atagtgacaa ggtattagtc gaatctcagc aatccttaca
    11221 tattgctact gttgtccttc ctaaaaaaga gattgatccc aatgaccttc ctgaagctat
    11281 ccgtccgatc ttgcataaag cgtcacaagc tgaattagaa aaagacaaaa aaaacaaaaa
    11341 agatgcttat gatgcattga aaatcgcaaa agaaaaagtg cagttgcgtg agttggaaat
    11401 gaaaatggtc aaagccgaat acacatttga tcgaaataaa ttgatttttt ctttcacagc
    11461 ggacgggcga attgacttta gagaattggt aaaagatctg gccgctgtat ttcgtactcg
    11521 tattgagctt cgacaaattg gtgtccgcga tgaagcaaag ataataggcg gaattggtcc
    11581 atgtgggcgt ccattatgct gttcttcttt tttaggcgat ttcgtgcctg tatcaatcaa
    11641 aatggcaaaa gatcaaagtt tatctttgaa tccaacaaaa atttccggtt tatgcggacg
    11701 cttgatgtgc tgtctgcaat atgagagcga agaatacgag acagcgaaaa aagaactccc
    11761 tgactttgga aaagtaattg ctacacctga tggtaaaggg aaagtcgtag ggttgaatct
    11821 gcttagccgt atcgttagta ttcggctgac tggacgggaa aatcctgtcg aatatgaatg
    11881 ggatgaattg aaagaattta tccaagtagg tgagatgaat ggataagaga tcattatacg
    11941 acggattgaa tcaattagaa tcagagttga gaaatacatt aacagaactt gttgaaatga
    12001 agcatgcttt gcatgagatc gtagaaaaaa atacaacttt ggagatggaa aatcaacgtc
    12061 tgcgggaaca cctgcgggaa ttgaaccaag ttgcacaagc tccagctgaa aatgtgaagc
    12121 aggaactttc taagtctcgt atgaatcttg aaaaaattta tgaagaagga ttccatgtct
    12181 gctacgattt atatggatca agaagggaca atgacgagcc ttgtgcgttt tgtctagaag
    12241 tgatctatcg cgaaagcggt cgatgaccaa taacaagagg agactggagc ggagaaatac
    12301 tgctccagtc tttttattaa ggagattgtg acagatgtat aatcaaaaaa gtttcaaggg
    12361 aaatccggta tatggggatt tatatcttgt accgacgcca attggaaatt tagaagatat
    12421 gagctacaga agtgttcgta tgctgcaaga atccgatctt atcgcaagtg aggatacgcg
    12481 taatacacaa aagttattga atcattttga aatccataca ccccaaaaaa gtttacatga
    12541 acataattat aaagaacgtg tccctcaatt gattgctttg ttaaaagaag gaaaaacgat
    12601 tgctcaagtc agtgacgcag gaatgccttc catcagcgat cctggacatg agctagtttt
    12661 agcctgcata aaagaaggga tttctgtgat ctctatccca ggacctacag ctgggatgac
    12721 ggctttgatt gcttcagggc tagtgcctca accattttta ttttatggct tccttccacg
    12781 taagaaaaaa gaacagaaag aaacattgaa aaagctaaaa gaacagacag ttacgctgat
    12841 tttttatgag tcaccttatc ggatcactgc tacattgacg aatttcttag aaatttttgg
    12901 aaatcgccaa gtcgttttgt gtcgggaatt gacaaaggtc catgaagaat accttagagg
    12961 aagtacggaa gagctactcg cttatatcga agaacatcca gtaaagggtg aatgttgtct
    13021 gcttgtagaa gggaatttgt tcgaaatgcc agtgacggaa gttgatgaag agttgggaac
    13081 gttaaaggaa caagtagaag caaagatcgc ttcaggcgaa aaaccaaatg ctgctatcaa
    13141 agcagtagcg ctgaaaaatg ggctgaaaaa acaagaagtc taccgtcagt accatgaatt
    13201 agactgagat agtataagtt gtctataatg aaagactaat gaggaaatca gctactaact
    13261 tttcaaatct cttgaacgcg aacgaatgtt cttgtataat gaataagagg tgaggatgat
    13321 ggagctgcaa tttcctttac ataatgatac atcaagaaaa atcattcata ttgatatgga
    13381 tgcttttttc gcttcggttg aagaacgaga tcatcctgag tttcgcggac atccattagt
    13441 aattgccaga caccccagtg acactggtgg gcgtggagta gtgactacag caaattatga
    13501 agcaagaaaa ttcggaattc attcagcaat gagtgcacaa aaagcgtatg agctttgtcc
    13561 gcaagccatc tttaagcctg gcgaccatca taaatacagc gatatttcca aacaagtacg
    13621 ggaagtattc tatcgttata cagatattgt ggaaccagtt agtatcgatg aagcgtatct
    13681 tgatgtgacc gaaaataaaa tcaattgcaa gtcagccatc aaaatcgccc gaatgatcca
    13741 agcagatatt tgggagagtg tccagctaac atgttctgcg ggagtaagtt acaataagtt
    13801 tttggccaaa ttagcatcag actatcaaaa acctagagga atcacggtag ttgcccctca
    13861 agacgctgtg ccatttttaa aagctttgcc aattgaaaaa tttcatgggg tagggaagaa
    13921 aactgtacct agaatgcatg agctagggat ttatacagga aaagacctat atgaatgcac
    13981 agaaatgatg ttgattcgaa attttggaaa aatgggctat tctctttatc gtaaagtcag
    14041 aggaatccat gattctccag taaacgttac aagagagcga aaatctgtgg gaaaagagca
    14101 tacttatggg cagccgttgc aaacggaaga agcagtgctg actcagttgc gccaactagc
    14161 tgaaaaagta gaagaagcac tgagaagagt ccaaaaacat gggaaaacag tagttttgaa
    14221 ggtcaggtac acggactatt ctactgtgac caaaagagtg acattgccag aatatatcta
    14281 taaaaaagaa gcactctttt atcaagcaag ccttatatgg gaggaaatct tgggagtaga
    14341 aaaaggaatc cgattgttag ggattacttt gacgaatctg gacccgatga cttatgaaaa
    14401 catcgtcctt ccattatggg aaaatcaaga aatataaaaa cagctttgag agttgaaacc
    14461 ggaaatccgt tttctctccc aaagctgttt ttatatcaag tcttgctttt tgatttgttc
    14521 ataggtttct gttgcatccg tacatttttc ccaaatcaca acttgattgg attctaaaga
    14581 tttttggacg tcaatgatcc gctggttgct acttccgcga aattgtagtt ttaggtcacg
    14641 tttgcttaat tcaaagcgtc cgtcaacaag gatatcgatt tgcgaaagga gttctaattt
    14701 atcttctgaa tcttgtagca attcatcaaa ggtatagcct gtccatgacc agatatcttt
    14761 tgtcgtaccg aactcttttc ggatacgtcg tacgacttgc agacaaacat ctgtattcaa
    14821 aaaaggttcg ccaccaagta aagttaatcc ttgcacgtaa tcgttgcgca agtcagcgat
    14881 gatttgttct tctaattcag gtgtgaatgg tttgccatat cgaaaatttt gtgcagcttt
    14941 gttgaagcag ccttcgcagg caaataagca gccactaacg taaagactgt tgcggacacc
    15001 ttcaccatcg acaaaattaa acggtttgta gtcagcaata tagttttggc tgtagtcttt
    15061 tgctaaccat tcttttggtt ttggatttct cataaaaaaa cttctttcat ttaaggttca
    15121 aagatatagc tattgaagat aaaatctact cgaccacata aataagaaca aactgaccta
    15181 aatccattgt tcaagaaagc ttgttcttat tttgatagag ggcgttcaaa gatacaaagc
    15241 catcgaaaga gggtggtgaa aaaagacttg tcatagcctc ttattctgaa taaacggtgt
    15301 tcaaaagctt gcatctgcag taataagccc aactaaacga aaaatatgag gaactatttt
    15361 tgtttagcta ttcttattac ttgatgcagg acagcttttt cacaacctca tttcatgtgt
    15421 ttcacacgtg aggaaatttc tttatggcgg ccatgtacca tcggacgagc ttgcggattt
    15481 cctaaatagc cacaagtacg tttgactaca tcacatgttt ttggatcatg atttccacat
    15541 tctggacatt cgaaccctcg ttctgtcggt ttgaaatcac cttcgaaacc acattgatag
    15601 caatgatcga ttggcgtatt cgtaccaaga tacccgattt tgtcatatgc aaaatcccat
    15661 acagcttcta aagctttcgg attttgttgg agcatagggt attcacagta gtggatgaat
    15721 ccacctgaac aatattttgg ataatccttc tcaaattcca atttttcaaa tggcgttggg
    15781 tttttacgca catcatagtg gaaactgttt gtataatatt ctttatctgt gatattttcg
    15841 atgatgccga atttttctgt atccaaacgg cagaaacggt cagttagact ttcacttgga
    15901 gttgaataga cactgaaatg ataaccatat tcatcccccc atgcatctgc attagctttt
    15961 aattctttga cgatatcaag tgtgaaagct tttgcttctg ggtttgtttc ccattcgcca
    16021 ccgaagaaag ctgaagcaac ttcatacaat ccaatgtagc ctaacgatac agtagctcga
    16081 cggtttttga ataattcatt gacagaatcg gtacgtgaaa gacgtttccc gaaagcacca
    16141 tacatatata aaattggcgc atttgctgga atggcttctt tacaacgttc tacacgatag
    16201 agaagcgcat ctttcatgat ttctaagcgg tcagacagta actgccagaa tttattctga
    16261 tctccttttg attcgatcgc gattcttggc aaattcaatg taacaacacc taaattcatt
    16321 cgtccgacat ttacttcttg accattttcg tctttccacc cttgcaagaa tgaacgacag
    16381 cccataggta ctttgaaact tccagttaat tcaacgattt tatcgtaatt cagaatatca
    16441 ggatacattc ttttcgttgc acattccaat gctagttgtt tgatgtcgta attaggatct
    16501 tcagggttta agttcacacc ttttttcaaa gtgaagatca gtttagggaa gatagctgtc
    16561 cgcttttcac tccctaatcc attgattcga atctgtaaaa ttgctttttg aatttctctt
    16621 tcaaaccagt tttctcctag tccaaatcct aatgaagtga aaggagtttg tccatttgaa
    16681 gtaaataagg tattgatttc atattccaaa ctttgcatag catcgaagat atcttttttg
    16741 gtttttgctt ttgcatattc tttttggcgt tctggcgaat cgatccattc ttccgcgtct
    16801 tttagatgtt ttttatagtt caattctgca aaaggagcaa gcaattcatc tgttcggtcg
    16861 gcagaacagc caccatattg acttgacgct acattggcaa taatctgtga gatttgtgct
    16921 gtggcggtct ggatagattt tggcgattcg acttcggcat tgccgatttt gaacccattg
    16981 ttcagcatgc ctttgaaatc aatcaagcag caattcgtca tcggtgtata aggatgataa
    17041 tccaaatcat ggtaatggat atcgcctttt tgatgcgcat ttgctatatg tggtggcaac
    17101 attttcagtc cgattgattt tccaacgatc ccggctgtca gatctcgctg tgtattgaaa
    17161 acattactgt ctttatttgc gttttcgttc acgacagttt ggtccttgtt gattagcttt
    17221 tcaatcgaaa agttgatatc tgttgcttta ctgcgagcaa aatctcgttt ggtacgataa
    17281 ttgatgtact cttctgctaa ttcatattcg tttttttcta gtaatatatg ttccacgatg
    17341 ttttgaatct catagatttt gacattttca gcaaaccggc ttttgatttc ttggacaacc
    17401 aagtctgcaa tcatttgaat tttttcataa gtaagtggtg ataatgagcc gtgaattttc
    17461 ttttctgctt tgatcagtgc ttcattgatc ttttgttcat cgaaatcaac atggcggcca
    17521 tcgcgtttga tgactttcat ctgggatgtc tctggctgct gagtaacgac agttttttct
    17581 tcataattta ctttcattat cttcaactcc tgctaaattg ttcttgtcta tcataaaaca
    17641 aaatcgggga aggagcaact aaatatcggc tatatatagt attcaatgtg ctgtataatt
    17701 ggatttctat actatatgta gtgtatattt tattgtgaaa aaaggtgaaa acttgtagct
    17761 ggtggatttg ttttttgcat atttaatttt gctgaataaa ataattgaat aaaatttttt
    17821 attcagtcca agtaaaagtg acaaaaacat aagagcaaat tattcgtaga tatgctacta
    17881 tgcaaataaa tagaaatgaa aaatgaaaga ggaggagaag aattggaaaa gatgatcgaa
    17941 gtgaaacatc taagtaaaag ctacggcagc aaactttctc ccgtcaaagc cttagataat
    18001 atctcattca cagtagaaga aggcgagttt gttgggatta tgggcccaag tggtgcagga
    18061 aagacaacgc tgatgaatat cttgtccacg atcaatttgc ctacgatggg cagtgtctcg
    18121 atccaaggaa aagaaatcac aaaaatgaaa aacagtgagc tcagtgactt tcgtcgaaaa
    18181 aaactaggat tcatttttca agagttcaat ttgatcgata ccctaaatgc aaaagacaat
    18241 atcctattgc ccctagcagt tgaacggatg actaaagagg aaatggaaaa gagagtaaga
    18301 catgtagctc aattgttgaa tatagaagaa ctgctcaaac ggtatcccga tgaactttct
    18361 gtcggacaga gacaacgaat tgcagctgct agagcacttg ttgttcagcc acaagtgatt
    18421 tttgcagatg aaccaactgg ctctttggac tcgaagtcgg ctacagaatt gttgaactac
    18481 ttgaagacca tgaatcaaaa agaaaaagca acgatactgt tagtcaccca tgatccatat
    18541 acagcaagct actgcgatag aattttattt attaaagatg gtgtgatttt ttctgaagtt
    18601 gtcagaagag ggacaagaag agagttcttt gagaaagtaa tagacatgca ggcgacgatt
    18661 ggcggaggag gaaaaatgaa tgctgtataa actagccgtt aaaagttttt ttcggcagtc
    18721 acgaggctat cttgtttatt ttttcagttt gactttatcc gtaatgatct attattcctt
    18781 cagcgcaatg acctacgatc aatctttagt cagaagagca agtcaagata cttcgataga
    18841 tggtggattg agttttggtg gatttatcat tactgttgtt ttgctttttt tcgtattcag
    18901 tgccaaccga tttttcttga atagaaggca aaaggaaata ggcatctatc agctttttgg
    18961 tatgaataaa ctgcagatat caggtatata tgtgttagaa atcatgataa ttggattgtt
    19021 cgcctgtatt tctgggatat tactcggtat tattttttcc aaactctttt caatgatttt
    19081 ggttcgaatg atggatatgg atcttactag tccatttttt atctccattc catcagtagt
    19141 agatacttta tttgcttttt tcatcatcct tttagcagta tctgtatatt caatatggaa
    19201 aatatggcgc tatccaatga ttcgaagttt tggagaaaaa gaacaagcag atagttcaac
    19261 aatgcgcttc aggacaaggc accgattact cgcagtcatc gggttactac ttttaacgag
    19321 cggatatttt ggagctagtc attttaggga aatcacttca tggatgatca gttatcatta
    19381 tcttgataca ttagttttct tttttccttt tttaattttt ttcgtttgtg tggtcggtac
    19441 ttatttgttt ttctgttata catttcggtt atttttgatc atattgagta aatggaagat
    19501 ttattactat ggtatcaatt ttttgataat gggtaacacg caagttcatt tgctcaaaag
    19561 ttggcgtaca aattctctga ttacagtcat tctcggaatt tctttaatga caattggcgg
    19621 cacggctagt atctctacta ttttatccta ccaggagaaa gtttcctccc cgatggacta
    19681 tcagtttgat gttgagacag agaaaaaaat caagccgatt ctagcagagg aagggcagaa
    19741 gataactaaa gaaatcacct tgcattataa agtcattggg agtatctata gtcaaaatct
    19801 cgaagaacct atgtttcaaa ctgctaatct gatcacggaa acacaatatc aagccttcag
    19861 aaaagttaat ccagatttgc cgaacatttc tttgaaatca cctggtcata ctgttttgct
    19921 tgatcaaatg caaactcttt ttcggaatat ttcattatat ggatctagta ttggactgcc
    19981 agataatcaa gaattgaaga ttcaacaaat gcttccaagt gtattaggtg acgaaacaat
    20041 ggtatatcta agtcctgtac tgattgtaga tgataccgtt tttgaaaaag caaaaggtgt
    20101 ggattatcaa gtagtcaatg tagatgtatc aggtggaaat aatgaaaaaa tagatgaacg
    20161 actgagcaat gacctgtcga taaagtggct caatgctgtt tattattctt atgatatcgt
    20221 aaatggaagt ctacaaggag aaattagcac aaagaaactt ccggatgacc aaatgaagga
    20281 agcacaattt agccaagaaa gctctcgttt gaatttctct tccagctatt cgaaattgcg
    20341 tgcagttaat cggcgaatcg gtatcaatat ttttgtcaca ctatttgtcg ggatgatcgt
    20401 gattattgct actggtagta ttttgacagt ccggcaatta gcagaagcag aggaagaaaa
    20461 agaaaattat cacctattga ctaagctggg gattccacaa agaaggatca gacggatgat
    20521 cttcgagcaa aacgcactta ccttctttcc acccatgata ttagggatca ctcacgcagt
    20581 atttgcaatc aatgtcttca ctcagtatat aaaaactgcc gattactggt tggcttatct
    20641 tgtgagtggc ttattgatcc ttatctatct tttgctttat gtcattacga gtcagttata
    20701 ttgtcggatt atcgaggaat gatatacgga ataagacaag ataataaata gaactaaatt
    20761 ttgaatacca tttatttgga ataagaggtt gtgacacgat ttttgtcaca acctctttat
    20821 tttttatatt ttttaaaagt acgagaaatt ataaagaaaa aagcagtgat ttgatgtgct
    20881 atttttataa aagctatgtg acaaaactgt tagtaaaaaa acggtttctg taagctagac
    20941 acatgtttct ttttgtcaaa attagttaca ctttgtgtga ggttatatga ggagggaatc
    21001 caatgaacaa atggcgttct aatccaaaat tgaattattg gggaaaattc atcattcgaa
    21061 caatttttta tagtgcagta ctgctgttcc tgatttatct gtatcactat aaaaatattc
    21121 aaggaggaac ttttatctat aacgaatttt aggaggaaga gtatggaaat caaaacgatt
    21181 attgaagcaa tcgatgattg gggaataaat gaacctgatc gagttgctta tcaggcggat
    21241 gagacgcata cttttggtga attgaaacaa gcatcagacg cacttgctta ttatttagaa
    21301 aaaaaggtag aaggcgatgg accaattgtc gtttttggca atttggagtt tgaaatgatt
    21361 gtctcatttc taggggttgt caaatctggc catgcctatt tgccgattga agagcataca
    21421 cccaaggagc gtatcctatc gatctttcgg gtggctaaac catctatggt catcagtatc
    21481 ggggactgga tagaagatat tccttcggtt cctgtcataa caaaaaaaga atttcaagaa
    21541 atcaagaaga taccagtagg attttatccc agaaatagcg taaaaggtac agaaaatttt
    21601 tatattattt ttacttctgg tactactgga gaaccaaaag gcgtacaaat cagtcatgat
    21661 aatctagtca gctttgttca atggacgatg gaagactttg ggatcaagcc tggtatgcat
    21721 ttcttagccc aagcgccatt ttcttttgat ttatcggtat tttctattta tcctgcatta
    21781 gtttctggcg gaatgctgaa gccgttaaat aaagcagttg ttcaagactt tcgtcaatta
    21841 ttcgcgacat tgccagcctt gaagttgaat gtgtgggttt ctactccatc atttatggat
    21901 atttgcttga tggaacctac attcaatgga gaaaatgtgc cggaattgga catgttttta
    21961 ttttgcggag aagaattgac gaaaaaaact gcagaaagtc tacttgatcg attcccaaat
    22021 gcacggatat ataatactta cggtccaaca gaagcaactg tagctatttc aagtattcag
    22081 attgaccagc atgtactaga tagttatgac cgactaccga ttggctacgt aaaaaaagac
    22141 acgaaagttt ctattgtcca agaaggtaaa cctgtttcaa aaggagaaac gggtgaaatc
    22201 attattgcag gaccaagcgt atcaaaaggt tacctgaaca atcccgaaaa gacagctgct
    22261 gcgttctttt gtcaaaatga cttagcaagt taccatacag gggatgcagg tagactagat
    22321 gaatatgggc ttttatttta tgaaggacgt atggatcagc aggtcaaatt acatggatac
    22381 cggattgaac tcggggatat cgaacattat ctgctccaag acaaccgaat caaacaagct
    22441 gtcgtcgtac caaaatacca aggaacaaaa gtacagcagc tggttgcttt tgtcgtatta
    22501 gaaaacaaaa cgaaacagcc ggattttcag ttgactaaat ctatcaaaga acaattacta
    22561 gaagttgtaa tggattatat gattcctcag aagttccatt atgtcgatct gctgcctcag
    22621 acagttaatg gaaaaatcga tcgaaaaaaa ttaacagctg aggtgaatcc tgaatgatgt
    22681 tacctcatat gatcccttat gcagagccgt tttatttttt tcttttagcc attgcgcttg
    22741 taccgatcgt tctatcttta ttgatatggg aaaaaagatt accagtttat caatcagcag
    22801 tgacactttt ctttttattt gtcagctttg gaggtaatta ttggcaacag ggagcggcgc
    22861 tgattgggta tgttttgtgg cagacgatac ttgtatgggg ctattttgtt tatcggcaaa
    22921 ggaaaaatca gacaggatgg ttttttgccg ccgttatttt agcgataatt ccactgtttt
    22981 taacgaaagt gactcctttt tttgaacatg gaaaagcatc gttacttggt tttttaggaa
    23041 tctcttattt aacctttaaa tctgtacaaa tgatcatgga aataagagat ggcatgatca
    23101 aaacatataa gccatttcgt tatatccagt ttctgctgtt ctttccaaca atctcttctg
    23161 gaccaatcga tcgctatagg agatttgaaa aagatcagct ccatccgcct gttcgtgata
    23221 aatatgttga tcttttggga aaaggcgtcc aaaatatttt tgtcggtttt ttatataaat
    23281 tcattatcgg ttattatttt ggtcaagtac tgcttcctgt agtgggacgc attgccttac
    23341 agcatggtgg tatttcatgg gcattagtag gttatatgta cgtgtacagt atgtatttgt
    23401 tttttgattt tgctggctat agcttatttg cagtcggtac aagctacatg atgggatatc
    23461 agacaccaat caatttcaat aaaccttttt tatcatggaa tatcaaagaa ttctggaatc
    23521 gttggcatat gacgctgtcc ttttggttcc gtgattatgt atatatgcga ttgatgttct
    23581 tcatgatgaa gaaaaaattt tttaaaagca agatcgtaat gtcaaatatc ggttattttg
    23641 ctttgtttct tctaatgggt atatggcatg gattgacgtg gtactacatc gtatatggac
    23701 tgtatcatgc ttgtctgatc tgtctgacag atgcatggat ccgatttaag aagaagcata
    23761 aagagcagtt gccttctaac aaatttacgc atggcttagc cgtatttttg acatttcaag
    23821 cagtatgttt tagcttcctg attttttcag gatttttaga taaacttttt tttagctaac
    23881 gaatagtgac atattaattt atataacaaa ggagaaaagg aaaatggaag aacaaatttt
    23941 atcaatctta gaagaaataa caggaacaga tgaagtaaga aaaaatcgtg atattgatct
    24001 atttgatgaa gggttgcttg attctttagc atctgttcaa ttattagtcg agctagacgg
    24061 agagctggat atccaagtcc ctgtatctga attcgagcga gaggattggt gcacaccgaa
    24121 taaaatcatt caacaagcaa ctgcattgaa gggttgagta tgaaaaagaa actttttgct
    24181 attttcggcc cggttgtttt agctgttggc ttactgtttg tgttttttac ctcctcctat
    24241 caagtagata tctctaatcc agaaacgatt agacgtgcct cttcttccat gtcagtgaac
    24301 gtgttgaaag gagatgcgat aaaaaattat gcattatctg aaaaacaata tattcctttt
    24361 tttggctcaa gtgaattaag cagaatcagt ccatttcatc cgtctgttct ggcagaaaaa
    24421 taccagcgaa actatcgtcc atttttgctg ggagcacccg ggacacaatc gttaagccaa
    24481 tatatgatga tgcgttctgc aggagatgct atgaaaaaca agaaagtcgt ctttattatt
    24541 tctcctcagt ggttcgttaa aaatggtgta aaaactgatt actttaatac gtattattct
    24601 gaacttcaaa catacgactg gttgttttca atgaagaaag taacgcctgc tgatcgctat
    24661 ttagctagaa gattattaac tttctcaaaa gtaaaagaga atgatacgtt gacggcgatt
    24721 ctgcaaacga tcaaaaaagg gaagctacct ttacctgaat ctttgaatca gctgcgcagc
    24781 caatggaaca tgctgaagag agaggatgaa gtattcagca atattgggtt gaaagatcga
    24841 cagcaaaaaa tcgatcatga gtcaaaacga ttaccaaaac aataccaaga aacagaatta
    24901 tccattttag caaatcaaat tggagaacga gaaacgacga ataatccatt cgggttgaaa
    24961 aacgattttt atacgcatcg tatccgagcc cacgagccag agctcaaaca gtcacaaaaa
    25021 aattgggatt atcgtttttc gccagaattt tctgattttc agctggtctt ggatcaatta
    25081 gcaaaaaacc ataacgaagt attgttcatc atcccaccgg ttaacgagaa atggagcgat
    25141 tataccggac tatctcaaga aatgttacaa ggatttgcca aaaagatcaa atttcaatta
    25201 aatagccaag gattcaatcg aatagctgat tttgtcaatc aagcgggtac caactatttt
    25261 atggaagaca cgatccatct aggctggaaa ggctggttgg ctgcagatca acagatcaga
    25321 ccgtttttag aagaaaatca tattactgct agtaagtatc atcttgacga tgcttttttt
    25381 agcaaatctt ggcaacatca aataccagat aaactacaat taaaataaag aaaacaggcg
    25441 agtgttgctc gcttgttttc tttatttttg aatattctga ataaaagata aaaataccaa
    25501 gtttgcctga acaaaaatca taagcaatgg tataatcaca atatgaatat agaaatgggg
    25561 gaaaacattt ggatacaaaa gaagaacagc tacttattga tttaacttct gcaaaaggag
    25621 tacctggaaa tgaagaagag gtaagagaag tattcagaga atatgccaaa ccattcgctg
    25681 atgatatttt ttatgacggt ttaggcagtg tgattgccaa acatggtgct ggcggaggcc
    25741 ccaaggtctt tatttctgga catatggacg aagttggttt tatggtaaca aaaataaccg
    25801 aaaaaggatt cttggaattc caaactcttg gaggttggtg ggggcaagtc atgcttgctc
    25861 agcaagttga aatcaaaaca cgtgaaggga aaatcgtcca cggtgtgatt ggttccaaac
    25921 caccacatgt tttaacacct caagtaagaa ataaacctta tgaaataaag gatatgttca
    25981 ttgatattgg cgcatcaagc caagaagaag ccaaagagtg gggcatacgt cctggagata
    26041 tggtcactcc atatattgaa tacaaacgaa tgaatggcag taaatattta ctggcaaaag
    26101 catgggataa tcgtatcggg acagctgttt ctctgcgagt attagagaat ctatctaaag
    26161 aagcacaccc aaatgtttta ttcgcaggaa gcgatgtgca agaagaagta ggcttgcgag
    26221 gtgctcgaac aagtacacac cttgtaaatc ccgatattgc ttttgcttta gatactggta
    26281 cagcaggaga cactccagga atgacaccaa aagaagcaga ttcgatttta ggaaaaggcc
    26341 cacagatttt gatttttgat gcatctatga tcccgcataa aaaattattg aattttgtta
    26401 ttgatgtagc agaggaaatg gatattccat tccaatatac agtgatttct ggcggaggaa
    26461 ctgatgcagg acaaatgcat ttgacaagag atggtgttcc gtctgtagcc attaccgttc
    26521 ctgttcgtta tctgcattcc catacatcag tgattcatga agatgattat ttgaatacag
    26581 tgaaactagt cacagaatta gtgaaacggc ttgatagtga aacagttgaa aaaattcgga
    26641 gttatgaata atgaataata gaatacctat ttctgaatat cggaaaaaag cacgagtttc
    26701 tttggaaaat caatggggaa tcaatacttg gcttgttttc ttatctataa tatttacgag
    26761 cgtcatccag aacttttttg acggtttcag tccttttccg gaagaaagca tgcagcagca
    26821 cttattgagt tttctagtcg ataatttact tttattcgca ttcaactatg cattgtttta
    26881 cattgcgcta gtagttgtac ggggtggcag accaaaaagt aaccttttat tttcagtttt
    26941 ccaaagaaag tattttggtt cgatacttgg catcaatttg atcaattcag tcatcaactg
    27001 gctactcaac agtttaatct ttctgcccat gtttttaatc agcggagtga gtacttatat
    27061 gcagctgctt ttcagttcaa atgcggtacc tacgggtgtc atgccagaag attttcttga
    27121 tatcagcttt gttttatcga tgatagggat gatttttgtt tcagcactca tcatgagtat
    27181 tgttggcggt ttattccaat ttgctgcatg gacaaagttc gactatcctg atttgtcggt
    27241 tattcaatgt ttgaaatatg gctggttttt attgaaagat agaattggta cgtatatttt
    27301 gttgcagctg tcttttatcg gctggtattt gctaggagca atcgctttgt tcatcggtct
    27361 tttgtgggtc attgtttatg tgaatgtgtc agttgcagaa ttttatgaac aagcacggat
    27421 tgaaaaagga ggtccagacg cctattttac ttagcatgta agcagtttat tcatcaaaaa
    27481 aataactttt gtggtaggat aaatctttga gggggaatgt acgatgaaat taactgtttt
    27541 aggatgctta ggagcgtatc cttataaaaa tcaagggaca acaggttatt tgcttcaatc
    27601 tgacgggttc aatttgttga tcgatgcagg tagtgctacg ctgatcaaat tacaagaaca
    27661 ccttgatcca cttgatctag atgctgtgat ccttagccat tatcatcatg atcatattgc
    27721 tgaccttggt gtattacagt attactggca acttcatcct aagagaaatc cggagacatt
    27781 gctttcaatt tatggacaca caaaagattc cattcacttt gaagaattga cgttggaaaa
    27841 tgtctcaaaa ggacatcctt attttgaagc tgaagaatta aaaattggtc cttttctgat
    27901 tacttttatg gaaacaatcc atccagtagt atgttacgcc atgcgtatcg tagaagaaac
    27961 aacggggaaa gtattcgtat ttacaggtga ttcaggttat ttggaaagtt tcgctgattt
    28021 tgcgaaagat gctgatttgt ttctagcaga tacctatctt tttgaaggaa atgaacatca
    28081 tcatgcccat ttcacttcaa aagaagctgg agaacttgcg aaatcagcta acgtaaagaa
    28141 acttgtactt acacacctac ctcagtttgg tgatttagaa caattgaaaa atgaagcgca
    28201 aaaggcagca ggcaaaggga ttgatataga acttgctgca gttgacaaag tgtttaacat
    28261 ttaggaggaa gaacatgatt tttgtaccaa atgaaaacca cgacccaaga atcaatttag
    28321 caattgaaca atatttgctg caagaaatgc ctttagatga acctattttg ctcttttata
    28381 tcaatgaacc ttcaatcatc atcggcagaa accaaaatac gcttgaagaa atcaatcgag
    28441 attatgtgga agaaaatggc attcatgttg tccgtcgctt aagtggaggc ggagctgtct
    28501 accatgattt tggaaacctg aactttagtt ttatcatgcc tgatgatggg gattcattcc
    28561 gtgactttgc gaaagtaaca cagccaatca tccaagcact tcatgaattg ggtgttgctg
    28621 gggcggaatt gaaaggccga aatgatctag tcattgatgg catgaaattt tctggcaacg
    28681 cgatgtatgc taccaacggt cggatgtttg ctcatggaac aattatgttc gactctgata
    28741 tcaatgaagt agtcaatgca ttaaaagtaa aaaaagacaa aatcgaatca aaaggaatca
    28801 aatcgatccg ttctcgtgta acgaatatca aaccatttct tccagaagaa aagcaaaaaa
    28861 tgacaacaga agaatttcgt caagaaatct tgttgaaaat tttcggcgta gagtcagtag
    28921 aagaagtaaa aacgtacgaa ttgactgatg aagattggaa aaaaatcaat cgaatctctg
    28981 aggaatatta tcgaaattgg gattggaatt acgggaagtc tccagatttc aatttctctc
    29041 gccaaaaacg tttttctatc ggttcgattg aagtccattt gaatgtctca gaaggaatga
    29101 tcaaagaagc aaaaatcttt ggcgatttct tcggactagg agatatctct gacgtggaaa
    29161 aaaaattggt tggacagaaa tatgataaag catctctatc aaaaatagtc agccaaattg
    29221 atgtgaagaa atattttggt gcgatagaac cagaagaatt gcttacctta ctttattgaa
    29281 aataaaaaat agcggaatcc acatatatgg attccgctat tttttataca ggattcgggt
    29341 aatttcgaga gagttgaaca gtgatccgat caatgaaagt atctttttcc tgcgttagtg
    29401 ttcctgtgta acgatccgct acgtctttca tcatcattaa gtcaaaacgt tcctgatcat
    29461 ctaattcttc tgtgatgcct cgactaaata ggtttgtatc gtaatgtgca ctatgttcgc
    29521 aacaaatgac gaaatgttcc tcttgttcgt gtatcgacaa ataaattttt cgatacagtg
    29581 gattttcgat ggcatgagtt tctctaaagg aatgctctaa agcgtggatc aaaagttgag
    29641 ccagatcgtc atcgttgata ggaagttctt cgggaagatc tgcttggaag gttatctgaa
    29701 tattccgttt ggcagctaac ttctggtaac gcgctaagat aagatttgtc acctggtggt
    29761 tgcaaaaaga acacagcatt tcgaaatttt ttgcttcttc taatatcttt tccacatagt
    29821 ttttagcttt tgtttggtcc gtatgcatta aatcttgcag attctccatc tcttggacaa
    29881 attccaggcg aagtgtattt acttcttgga tatgctgtcg aagctgctca taatgttgct
    29941 ccaacaaatc atttttgatt tttaagaaat ctacttgtct ccgatctttg tcggtattta
    30001 agatgtgatc gattagattg tagctgataa taacgattag aatgataata tataaaatcg
    30061 tagaccaatt gatcgttgtt tgatagatac ctaatgccgt ttggatgtaa atcaagcaat
    30121 ggataatggc taccaagacg atccaaggtg tacaagctac ataaaaacgg ttattcttat
    30181 atgcttcgcc aatgcatgca agtgaagtga ctaaagtgct tataacacta gcaatattag
    30241 caatcggcat taattcagga agttctgcag gagtaaaagt tgcataaagc agaacgccga
    30301 taaaaatcaa aagttggaac gtacaaaaaa ttccgtacca ttttttatag taaaacattc
    30361 ggctccaata gtagcagatg atgaaagctg acgttaagat aataaataaa tgagataaaa
    30421 aagaatgaac gaatggttca aaaagaatac cacttaaaat atcttctgat acagcttcaa
    30481 atcctaatgc caaagcaaag caacttaaga gaataagtga gtattcaaga cgtttttgtt
    30541 tatacatcaa gtagatcgta tagaaaaagg tccctatagc gataaaaaca gcgattatca
    30601 tcgtcaatac ttgcggcact gattgtgaat aaataaaact aatcaatggt ccagttgttc
    30661 ctagataaac tttcggtggc agtccagcat agttctgata aggagatgag actgtaaggg
    30721 tcaatttttt tccgatataa tttgctggta aatctatcac agccaatgag agtccgggat
    30781 tgctttcttc ctgataatct ttccgttggt ataaaatctt ttcgcctaga tatacagtaa
    30841 tccattgatg gtttcctttg attagtaaat ttgcttcgtc caacggtttt tcaagtgtcg
    30901 tttccataat gaatgtttcg tctttatcca cggtaggaat cgtacgagtg tacttgctgt
    30961 caaacttaaa atcaggatca tcttttgtat agaaaaccca cgaatctttt atttctttca
    31021 tttgattgct gtccgcagga gaaaaaataa aataaaagaa ataagcaatt cctgttaaaa
    31081 ataacaatga taatataatg attatgctcc actttatacg taaataaaaa gattcttttt
    31141 tattatatat gttattaaaa aaagacataa aggtattcct ttctaaaact tgtaatgtta
    31201 ggg
//
LOCUS       DAYYGO010000019        28649 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000019 DAYYGO010000000
VERSION     DAYYGO010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.019"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..888)
                     /locus_tag="ACHA5T_000763"
     CDS             complement(<1..888)
                     /locus_tag="ACHA5T_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8886361.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKG"
     gene            complement(1017..1781)
                     /locus_tag="ACHA5T_000764"
     CDS             complement(1017..1781)
                     /locus_tag="ACHA5T_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8886362.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            complement(1785..2174)
                     /locus_tag="ACHA5T_000765"
     CDS             complement(1785..2174)
                     /locus_tag="ACHA5T_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886363.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            2309..2851
                     /locus_tag="ACHA5T_000766"
     CDS             2309..2851
                     /locus_tag="ACHA5T_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFM8886364.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            2999..3631
                     /locus_tag="ACHA5T_000767"
     CDS             2999..3631
                     /locus_tag="ACHA5T_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886365.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(3765..3890)
                     /locus_tag="ACHA5T_000768"
     CDS             complement(3765..3890)
                     /locus_tag="ACHA5T_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFM8886366.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(4024..4917)
                     /locus_tag="ACHA5T_000769"
     CDS             complement(4024..4917)
                     /locus_tag="ACHA5T_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFM8886367.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(5099..6025)
                     /locus_tag="ACHA5T_000770"
     CDS             complement(5099..6025)
                     /locus_tag="ACHA5T_000770"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFM8886368.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(6110..6871)
                     /gene="pflA"
                     /locus_tag="ACHA5T_000771"
     CDS             complement(6110..6871)
                     /gene="pflA"
                     /locus_tag="ACHA5T_000771"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFM8886369.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(7115..9346)
                     /gene="pflB"
                     /locus_tag="ACHA5T_000772"
     CDS             complement(7115..9346)
                     /gene="pflB"
                     /locus_tag="ACHA5T_000772"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFM8886370.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(9623..12073)
                     /gene="parC"
                     /locus_tag="ACHA5T_000773"
     CDS             complement(9623..12073)
                     /gene="parC"
                     /locus_tag="ACHA5T_000773"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFM8886371.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(12085..14130)
                     /gene="parE"
                     /locus_tag="ACHA5T_000774"
     CDS             complement(12085..14130)
                     /gene="parE"
                     /locus_tag="ACHA5T_000774"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFM8886372.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(14296..14730)
                     /locus_tag="ACHA5T_000775"
     CDS             complement(14296..14730)
                     /locus_tag="ACHA5T_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFM8886373.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            14857..15495
                     /gene="plsY"
                     /locus_tag="ACHA5T_000776"
     CDS             14857..15495
                     /gene="plsY"
                     /locus_tag="ACHA5T_000776"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFM8886374.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(15627..16502)
                     /locus_tag="ACHA5T_000777"
     CDS             complement(15627..16502)
                     /locus_tag="ACHA5T_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFM8886375.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(16584..17375)
                     /gene="codY"
                     /locus_tag="ACHA5T_000778"
     CDS             complement(16584..17375)
                     /gene="codY"
                     /locus_tag="ACHA5T_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFM8886376.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(17393..18793)
                     /gene="hslU"
                     /locus_tag="ACHA5T_000779"
     CDS             complement(17393..18793)
                     /gene="hslU"
                     /locus_tag="ACHA5T_000779"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFM8886377.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(18807..19355)
                     /gene="hslV"
                     /locus_tag="ACHA5T_000780"
     CDS             complement(18807..19355)
                     /gene="hslV"
                     /locus_tag="ACHA5T_000780"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFM8886378.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(19392..20297)
                     /gene="xerC"
                     /locus_tag="ACHA5T_000781"
     CDS             complement(19392..20297)
                     /gene="xerC"
                     /locus_tag="ACHA5T_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFM8886379.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(20495..21871)
                     /gene="trmFO"
                     /locus_tag="ACHA5T_000782"
     CDS             complement(20495..21871)
                     /gene="trmFO"
                     /locus_tag="ACHA5T_000782"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFM8886380.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(21840..23918)
                     /gene="topA"
                     /locus_tag="ACHA5T_000783"
     CDS             complement(21840..23918)
                     /gene="topA"
                     /locus_tag="ACHA5T_000783"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFM8886381.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(24040..24897)
                     /gene="dprA"
                     /locus_tag="ACHA5T_000784"
     CDS             complement(24040..24897)
                     /gene="dprA"
                     /locus_tag="ACHA5T_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFM8886382.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(24953..25720)
                     /locus_tag="ACHA5T_000785"
     CDS             complement(24953..25720)
                     /locus_tag="ACHA5T_000785"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFM8886383.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(25713..26573)
                     /gene="ylqF"
                     /locus_tag="ACHA5T_000786"
     CDS             complement(25713..26573)
                     /gene="ylqF"
                     /locus_tag="ACHA5T_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFM8886384.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            26833..27231
                     /locus_tag="ACHA5T_000787"
     CDS             26833..27231
                     /locus_tag="ACHA5T_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8886385.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            27468..28010
                     /locus_tag="ACHA5T_000788"
     CDS             27468..28010
                     /locus_tag="ACHA5T_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFM8886386.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(28093..28644)
                     /gene="lepB"
                     /locus_tag="ACHA5T_000789"
     CDS             complement(28093..28644)
                     /gene="lepB"
                     /locus_tag="ACHA5T_000789"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM8886387.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
BASE COUNT         7681 a         6048 c         4914 g        10006 t
ORIGIN      
        1 ccctttttct tctatttttt ttaacaacat ttctccacat acttcaccca tacgtataat
       61 atcttgcttg atcgttgtca atttaggtga actgatttgg tctaaaaata ccccatcaaa
      121 tccaatcaca ccaaattctt cgggcaagtg tcctccagca tttataatcc ctcttactgc
      181 tcctagtgct agccgatctg aactacatac aaaacaagtt ttatctgtaa aatctgccca
      241 gttttctgtc atatatcttt ctacttctgt cgaatgattt ggaaaccgca agattttact
      301 aggaagatca acttccttca tcacagataa ataaccagtt tcccttgaaa tttcaaaagg
      361 ctcgtccact tcgataccga tataaataat cgtttgataa tctctttcta aggcatattg
      421 cgttgctttt gctgtaccat attcattatc gctgtctaca taatcatatc cttcagaatt
      481 ttccccaaac aagacaactg gtttttccaa acggtcgatc caatcgaagt ctttttttct
      541 gacacctgtg atgatgtatc catcacaaga accgacgtcg aaggcatcat ttgtaactaa
      601 ttggagagaa taatgcgctt taccgatccg tttagcgatt cccatcagca aattcatgta
      661 atagggctca gttgtgtcga tttcttccag tataaataat ttgatgatct gcgaccgatt
      721 cgtgacaagt gcttttgcag caatatttgg acggtaatcc agttcagcca ttgctgagaa
      781 aactaactct ttcaattcgt cagtcacctg ttccggatga ttgatgactc gagagaccgt
      841 catttttgaa acattggcac gttttgcaac atctgctaaa gtgggcatgc tctcttcctt
      901 tctaacatgt tgtcattctt actatcaatt ataccaaatt ttcctaacag agtttattat
      961 agcccaaaat aaaaaacgga aaatcctcta tcgtcatgag tgattttccg tttcaattat
     1021 ttatactttt ttatcaaatt ttttgctaaa aaagtggaag cttcttccat tagtggtatt
     1081 tgtaccatat gtcgctgtgt ttttgaggaa cgaaaaacaa tttcttttcc gtaagactta
     1141 tgccgatttt tttctacaaa tcgttccgtt tgtatataag ggattttttc gtcagcttgt
     1201 ccatgccaaa agaacagagg acgtccagct aacttttccg gctgcaatga caaatcatag
     1261 ctcttaaccc atgaaatcaa atcaaaatag tcttttggta cgacataatt atattccgat
     1321 gcgcgcctta cgatttcttt tccatattcc aaaggtgcag gggaacccat caaacacacg
     1381 cccgctacta tatgaggatg tttagccaac aaggcgcatg ttgtcattcc ccccatcgaa
     1441 accccgccaa ctgcaagctg atcatgtaga agacctcttt tttctaaatg cttgataagt
     1501 gtgtcaaatt caaacagatt tccgtagata ctatcccaaa atgtatagga tggaatagaa
     1561 gaaaccggct gtttgcgctc cccatgattc atcgcatctg gcaggatcac acgaatgccc
     1621 tgctgcgcaa tttttcttcc ttgcgtcaaa actaactcct tacttgtctg ccatccgtga
     1681 taatagatga caacgggaat acgttcctct cttaattctt tcagaacaac ttctaacact
     1741 ggaatatgat ggacagaacg tttcaatata attggattca attgttactc acctattttc
     1801 attaaatttc ctgatcgcca tagcaaccat gctccaacag ttgtctggaa caggttcagt
     1861 gtaccaaata atataaatgc aggactgaat ggctcgggaa ataagcacag agaacaaagt
     1921 cccaccactc ctaatgctag cgaaaggagt ggataaatag aaaaattcaa aaaactggca
     1981 acgctaccga aaatattacc aataaacagt atcaaaaaga aaaaaacgct taaaattaaa
     2041 ccatctgtca gcgacatctg aaaaacaatg ttctctacct caattggctg ttgaatcgaa
     2101 tagacaccta agtatatact gagaaggaga cttagcacgt ggctggcaca aaacacacga
     2161 ttgaccgttt tcattttaat cacctaacct atctttataa ttctattata atcattttat
     2221 catttttttg tatgtataaa gtttcaattt ttcttcactt acgatttttt ttgatattgt
     2281 tgttatataa atgaaaggag ttttacaaat gattgatcca aaaatgttgg ggatgatctt
     2341 cctcattaat tttgcttata tcactctgaa cacgttacgt ttcatgttga caatgaaagg
     2401 ctatcgactg attgctccac ttgtcagtat ggtcgagatc acgatttata tcgtcggctt
     2461 atcccttgtt ttagatcgtt tagataatcc actgaattta tttgtctatg cgttaggtta
     2521 cgcagtaggt atcagtgtcg gaattttgat tgaagacaaa ttggcattag gatacatcat
     2581 ggtcacaacg attttgccag caaatagcgc agaagataaa agtttgccgg gtatactgag
     2641 agaacacggt tatggtgtga cacagacttc tgcaatgggg ctggaaggtg agcggctagt
     2701 tctcgaaata ctttctccta gaaaaagcga acgtgaactg tatcgcctga tcaaagaaat
     2761 cgaagagcgt gcttttatca tttcttatga gccaaaatat atttcaggtg gtttctggac
     2821 taaaaaagtg agaaaacgac aaggaaaata actcacaaac ttaaaaactg tctaatatct
     2881 aaattaaatt ttaaatgtta gacagttttt atttattatt ttgattaaaa aacatcgaca
     2941 tatactcatc aatttgttat tatactttat atacttatca atttaatttg gagatattat
     3001 ggaaattcaa cgtagaacca ctaatttttt acatttaggc tctttttttg caactttgac
     3061 gatcttattt tttctatgtt attcatacct taagacctca catgcgcctt ctagtgcatt
     3121 aggctggttg cttttggatc attctgatat cacagaagcc gaagaaaatc cgtttttttt
     3181 gattttgtct tctctttgcg gcttactgtt ttctgtttat ttcggtgcct atttttttca
     3241 tcgacatcct ggtacgttaa acgaagcttc aagcgtattg attgcattta aaacgttttc
     3301 ttttttagca attctttttt attcgtttaa tcacaatacg cttttcttct ttatcatgaa
     3361 tggtttagtc gtgattttat ccatttttac ttattttatt ttttcactca tttttagcca
     3421 attgaaatgt ccgctcttct ttcgattagt tcctgtcacc tgttttattt acagcttgtt
     3481 ttcctttttt gtccttgctt taatgccact gaccaaccct tcgacgatcc aagtcgcagg
     3541 aaatctgttg gatatgctct tcatcgtcag tctaggtgta tttatgtggc aaagaaaaaa
     3601 caaacaaaat aaaaagcatt ttgaagcctg actattacga cttcataaat gcttttttag
     3661 ttttaaacac ttcaatctaa aagaataacc caagattgga taaattatta atgactcgcc
     3721 attcgtttct tagtgattct ggattatcaa atggacttcc taatctaatc agctagttct
     3781 tcccataaaa tgccatactt gactgaatca taatattgat tatttaaaaa tctaactttt
     3841 cttatcacta cttcctttgt cattccacat ttctcaccat ttttttgcat tcctttgttt
     3901 ccagaccatg tagtgaatct aataggtggt agataggcaa aatactcaaa taattctttt
     3961 aaccataaag gaagagaaag gctcctcctg caagagtcag tataattttt gcttactgct
     4021 ctcttaggtt ttactcacta aaactgttcc atattctttg atataagaaa atgcatgcgg
     4081 tataaatttc ttgctcagct cagaaaagat ttgtcgttta agaaaggcca tttcctccat
     4141 tgtcaatgaa ggatccaatg ttggtaattc aatctcaaaa ttgttttttt cgacaatagg
     4201 taattctaat gtgaatgatt cttcttcctt tatagtattc gtcacaagta gagactgatg
     4261 gaaaatacta ggccggcgat ttttatattt attcttacgt atcctgtcaa cgataacttc
     4321 tatttgatca aaaaagtacg attcatttac gtctccaata agttcggcta attctttgaa
     4381 tgccttcgtt acaggaagct gattcttctg tacaaaaaag taattagcca aagtaagcgg
     4441 gaagtgttct atagaggaaa gtgtgataaa acggtacttc tcatttcctg cagttgtcct
     4501 cagcaattct gtctctcggc tgtttcttac tccataaaaa tacgcttctt tccgttcatc
     4561 ttcgaaacaa tacgcccaaa taacttctcc catcgctaat ttttgttcat caacagagag
     4621 tctcttcaat tgttcagcta tggtttcagc agccgaagtg gcttgttgca agtaatcttt
     4681 aggatcaaat attggaaagt tcaaatgata tcttcgttct tccctcttga ttaatcctgc
     4741 atcgatcaat aaatcaagaa aatgggaaaa tttattttgc gaaaattctt ttttcaattc
     4801 tcttagaatc gtttcttgtt ctcgatgttc atatagataa tcggtcaatg aagaaaaaag
     4861 cggatcatcg gtcagttttt gataaaagtt ttccttattt tttgaataat agttcatata
     4921 tgttctcttt tctatccgtt ttattttact taaaaggctt ttacgtaaaa aggaggaaga
     4981 acaagagtca taattgactg ttattctcct ccatttcttc ggcctttagt atgttctatg
     5041 tttcttttta tatcaatcgc ttatccattc attttgaatg cttcatacta ccatttgatt
     5101 agctaaatac agccgtcaat tgaggtacga cttgtttttt acgtgaaacg acacctttaa
     5161 gcaaagcacg gttgttggtc aaagttgttt ggaaagcttc ttctactttt tcttttcctt
     5221 taccagcaac aagtaattct gaatcactgt ctaagatgtt tgtgacaact aatacgaaca
     5281 gatcgtagcc atttgctttc atttcatctt ccattgctgc ttctagctct gcttgtcgat
     5341 ccatcacttc attaaggtcc actgtattga cttgagcgat acgaacgttg gaactgccca
     5401 ttgggaaact ttttgcatcc aaatcaagaa gaaccgcagc tgatttatca cttagatttg
     5461 ttcctgcttt caacatttca agtccataag tgtttagatc gacttcagcg attgctgcaa
     5521 gttcttttgc agctgcttca tcttcaggcg tacatgttgg tgatttgaac aacaatgtat
     5581 ctgaaatgat agctgagagc atgattcctg cgatttcttt aggaattgtt acttggtttt
     5641 ctttatatag ttttaaaata atcgtacttg tacatccaac tggttctgca cgatagtaca
     5701 atggattagc tgtttcaaag tttgcaatac gatggtggtc tactacagct aaaatattta
     5761 cttcgctgat atctgaaata ctttgttgga attcattatg atccacaagc atgacatctg
     5821 cattttctgc tttagctgac tctactactc gtggtgcaga tagctgaaag tggtctaatg
     5881 cgtattgtgt ttcttcatta ggtgtaccta gagcaactgc ttctgtttct ttacctaatt
     5941 cattttgcaa gtgtgaaaac gcaatggctg ccccgattgc atctgtatct ggattttggt
     6001 gaccaaaaac taaaattttt gacatgtcat tcgctccttt gtgatttctc ctatttaata
     6061 atagcaaaaa aacggaaaga agcaatactt atcttctttc cgatgcgacc tatcgcgtta
     6121 aatattttgt atactcttct gtatgaagca atcgtttagc attttcgaca cgttcttttg
     6181 taggtggttc gatcccttct aatggatatt tcaaaccaag tgtttcccac ttgtattttc
     6241 ccatcgtatg ataaggaaga atctctactc tgtctacatt tgacaaggtt tgaataaagt
     6301 tgtctagtcg gatcaaatat tcatcataat cacttcgctc aggaactagt acatggcgaa
     6361 tccaaaccgg tttgttgatt tttgacaaat attgagccat ttctaaaata ttttcattgt
     6421 cccaatgcgt tagtttttta tgctcttcat tatcgatgtg tttgatgtca aataacagta
     6481 aatctgtata cttcatcaat tcttgaaaac gtgaaaagaa aggttcttca tacgtaaaag
     6541 gttttccgca tgtatccaat gtagtatgca tcttccgctc ttttgctttt ttaaatagtt
     6601 cgatcaaaaa atcgatctgc aatagaggtt ctccaccact aacagtaata ccacctttat
     6661 ctccccagta aggtctgaag cgttctgctt tatctaaaag ttcatctgct gtgtattctt
     6721 tccctccgcc aatattccaa gtatctggat tgtgacaaaa ttggcaacgc atgcggcatc
     6781 cttgcataaa cacgacaaag cgcagtccag gtccgtctac tgaaccgaaa gtttcgatag
     6841 aatgaacata gccgattgtt ttttcttcca tttaagttca catcttttct ttgatttcat
     6901 tttaagtaca agttagctgt cgaagcgaag ctttctgaac aaaatttttc gcaattgccg
     6961 cttcgttatg tgtaaaattt acagggacgg aacatacaag tcctgcccct gtaaatcgtt
     7021 tctttcaaat aagcatactg attattcgat tttgatgcga tggctattca acgtcctttc
     7081 gacgctaagt cgaaaaactt ttttcacgaa ctttttacat tctgtcgtgt gaagttctca
     7141 tcaagacatc taattgttgt tctcttgtca agtcacggaa tttaactgca taaccagata
     7201 cccggatcgt caagttcgga tatttcattg gattttccat tgcatcacgc aataaatcat
     7261 tactgaatac attgacattc aaatgataag ctccttttga gaagtatcca tctaagacat
     7321 tacgcaagtt ttcgattctt gtatcttgtt cttttccaag acctgaagga ttgattgttt
     7381 gggtatttga aattccatcc aatgcacagc cgtaattcat tttagctgtt gagttcaggc
     7441 tagctaataa cccactttgt tccgcattgt aacttggatt tgctcctggc gcaagtggtt
     7501 ttccagcttc acggccatca ggagtatttc ctgtattttt accgtaaaca acatttgaag
     7561 taatcgtcaa taatgaagtt gttggttttg agttgcggta agttttatga cgtttgattt
     7621 gtcccatgaa gtattccaag atccatgaaa ctaattcatc tgcacggtca tcatcatttc
     7681 cgtatttagg gaagtcgcca tccacttcaa aatcaactgc tattcctttt tcattacgga
     7741 ttgcttttac ttttgcatat ttgatcgctg acaagctgtc tgccgcatga gagatcccag
     7801 caatacctgt agcaaatgtg cgttgcaagt cagaatccat caaagccagc tgagctgctt
     7861 cataagaata tttatcatgc atataatgaa tgatgttcaa cgtattgaca tatagttctg
     7921 ctaaccaatc catcatattc atgaagttat gtttcacttc ttcaaagtct agtatttctg
     7981 atgtgattgg acgatattct ggtccaactt gggcgccagt cacttcatcg actccgccat
     8041 tgatggcata taatagtgtt ttagccaagt tagcacgagc gccaaagaat tgcatgtctt
     8101 ttccgacaac tgttcctgat acacagcaag caatcgcaca atcatcagaa ccccattgtt
     8161 cacgaagctg attatcattt tcgaattgaa tcgaagaact ttcgatagcg attctgctag
     8221 catatgttct gaatccttct ggaaggtgtt cagaatacaa aacagtcaag tttggttctg
     8281 gtgaaggtcc catatttgtt aatgtatgaa gcagacggaa atcattcttt gtaactaatg
     8341 aacgaccatc taatcccata cctgcaatgg ataaagtagc ccagataggg aatcctgaga
     8401 ataattggtt atattcaggt gtacgcgcga atttgaccat acgtaatttc attactaagt
     8461 ggtcgatcaa ttcttgtgct tctttttctg tgattactcc attatccaaa tcacgttgga
     8521 tatagatatc aaggaatgct gagatacgtc cgattgacat cgcagcacca ttttgtgatt
     8581 tgattgctgc taaatatccg aagtatagcc attggacagc ttcttttgca tttgcagctg
     8641 gtttagaaat atcatagcca tatttcgcag ccatttcttt catttgcgct aatgcacgat
     8701 attgttcagt gatttcttca cgtaaacgaa tcacttcttc actcattgta tgataaccag
     8761 tcatatcatg atcttttttc ttttgttcca tcaaatagtc gattccatac aatgccacac
     8821 gacgatagtc accgatgata cgtccgcgac cgtaagcgtc tggaagccct gtaatgattt
     8881 tgtttttacg agcagcacgc atttcagcag tataagcgtc aaatacacct tgattatgtg
     8941 ttttgcgcca gtcagtgaaa atatgtgtca attcatcgtt tggtgtatag ccattgcttt
     9001 ttagcgcatt gttagccata ttgattccgc cgaacggcat gaatgcttgt ttcaaaggtt
     9061 tgtctgtctg tagaccgacg attttttcca aatctttatc caaatagcct gcatcgtgtg
     9121 aagtaatagt tgatacgatt tctgtgtcca tatcatagac accgttacgc tcgaattgga
     9181 tatcatttaa cttttgcaat tcatcccata atttttctgt tgcttctgtt gggccttcca
     9241 aaaagctgtc atcaccgcga tattcagtgt agttattttg aataaagtct ctagtgtcta
     9301 cgccttcttt ccatttggta cctttaaatc cattccattg ctccattgta tggacctcct
     9361 aaatttctgt tttatgtaac agtttttacg tgattagtat aacacagcac cctatctttg
     9421 caagggtttt cttgtttttt atttaacaat ttttatgttt tatttacata tatttaaatc
     9481 aacgttgaca taccaacatt taagcattgt gaacactttc atcttttttt gcaaacgtat
     9541 aaggatttat taaaaatgaa tgaaaaagag acgccacctg tattaataca gatggcgtct
     9601 cttcattaaa atactacaac agttacgact cgatgaaaat ctcaggagca tcgataactt
     9661 cgatgacttc tccacctttt ttctcatcca tcgcaaatga tccattcgat gttcgatcac
     9721 ctatagggaa atcagttggg tcaatcgtta actgctgccc tttttgattc atcaaggtta
     9781 tttcttttga tcttccttcc gacatataga taatacggtg cggatttttt ttcagctcac
     9841 gcatgaccgt cactccccgc ttcgcacgtg aagtttggct gatttcttga gcaagcatgc
     9901 gtttaacagc tcctcgctga gtaacgatta cgatcggcgt atcaccttct gctaacacaa
     9961 gcagcccgtt gataatccta tctccatctt tcaaattcat agacttcact ccagctgcct
    10021 ttgctccaac aacgggaaca tctgttaaag gatagcgcaa acccattcct tgatgcgtta
    10081 ctaagaaaac atctaagcct ggctgatctt ctttcagata cacagcaatc agttcatctt
    10141 cttccgcctt tagcttcata cagctgagcg gacggctctt ataggtgcgc catggttcga
    10201 actctgtcat tcgtgtttgc ttgatcaatc cattcttgct gatgaataca aatgttttct
    10261 ctgcttgaat tttttgatat gggaaaacag ctaaaatcga ttcgtctaca gacagattgg
    10321 agattgtttg agaaatgtgc tcaccagcat ctttccattt caaatcgggt agttcatgga
    10381 cagggcggta gatcacattt gctttatttg tgatcagaag aacatggtcc aatgtactca
    10441 attcacctga gtatagcaag tagtcgcctt ctcgcatccc gatttcttct ggtttggaag
    10501 cactataaga acgaaggctt gttcgcttca catagccttc tcgcgtcaca ctgacaatta
    10561 cgtcttcttg tgccactaag acttgcgtat caattttgat ttcttcgatt tcgtcttcga
    10621 ttgtcgttag acgagcggaa gaataatttt ttttcacttc tcgcaattct tttttcataa
    10681 cagagaaaag ctctttgtca tttgaaagga ttttattcaa ctctgtgatt tgcgcaatca
    10741 agctttcgga ttccttttga agctctgtga tatccgtgtt cgtcaaacgg tataattgca
    10801 atgtgacaat tgcttctgct tgttcttctg taaattgaaa gacgtcgact aaattctttt
    10861 tggcgtcttt tttgtcttta ctttcacgaa tcgttgcgat tacttcatcc agaatcgata
    10921 aagctttcat caatccttct acgatatgct gtcttttttg agctttggct aaatcgaatt
    10981 gactacgttt tgttatgact tgctttcgat gctcgatata acttcttaaa atgtctttca
    11041 gtccaacttg atgcggagtc atatggtcga tagcgaccat attgaaattg taattgatct
    11101 gtagctcagt gtttttgaac aaataattca aaattccttg agcattggcg tcttttttca
    11161 attcgacgac aatttgcaga cctgttctat cactttcatc tcgtacttca gctattccat
    11221 cgattttctt attcaaacgg atctcatcca tttttttcac caaggtcgct ttatttactt
    11281 catatgggat ttctgtgatc acgatttgct gtttattacc cttcattgcc tctatctttg
    11341 ttttcgaacg aagaataact tttcctcgtc ctgtttcata agcttttttg atttcatctt
    11401 ttccttgcaa gatccctcct gttggaaagt caggtccagg aatatattcc atcaattttt
    11461 caaggctcgc ttgtggatga tcgatcatat agattgtgcc atcgatcact tcagctaagt
    11521 tatgagtcgg gatttctgtt gcatatccag cagatatacc tgtcgatcca ttgacgagaa
    11581 ggtttgggta tcgagctgga aggactgttg gttctttttc tgtatcatca aaattccaca
    11641 cgagatcgac tgtttctttc tcgatatctg ctagcatctc tccacttaac ttggataatc
    11701 tggcttcagt ataacgcatc gcagcaggtg gatcaccgtc catactcccg ttattcccgt
    11761 gcatttcgat cagtacttcc cgcaacttcc agtcttggct catgcggacc atcgcttcat
    11821 aaatactgat gtcaccatga ggatgataat tccccatgat atttccgaca gattttgctg
    11881 atttacggaa tcctttgtca tacgtattgc catctttgtt catggcaaat aatatccggc
    11941 gttgcactgg cttcaatcca tcacggatat caggcagtgc acgttcttgg ataatatatt
    12001 tggaataacg tccgaagcgg tcacccatca cttcttctaa agttaattct tgtacttctt
    12061 gtcgattttc catcaggtga cctcctattc tacttcaaat aactgattgt tttcattttt
    12121 atctgctcgt tcttcatcta ataaatcatt agaaactgaa ggagagattt cagtatcctc
    12181 ttttttatct aagatactgc catcttcttc gagcgtaaat tgcacgtgat tttcgatcca
    12241 ttttcttcga ggttctactt tgtcacccat cagtgtggtt actcgacgtt cagcttgcgc
    12301 agcatcatcg atacggacgc ggatcaacgt acgggaagtc ggatccatcg ttgtttccca
    12361 caattgttcc gcattcatct ctccaagtcc tttatatcgc tgcagcatat atcctttgcc
    12421 gactttttta atcattgctg ccaattcgtc atctgtccac gcatattcaa tgacttgctt
    12481 ctttccttgt cctttggata ctttatataa aggcggtaac gcgatatata ctttcccagc
    12541 ttcaatcagc ggtttcatat aacgataaaa gaaagtaagc agtaatactt gaatatgggc
    12601 gccgtctgta tccgcatcgg tcatgatgat tactttgtcg taattacagt cttcgataga
    12661 aaactcagga ccgactcctg caccaatcgt atagatcatc gtattgattt cttcattttt
    12721 caagatatct tgcattttag ctttttcggt attgatgact tttcctcgta aaggtagaat
    12781 tgcttggaac tttctgtctc ttccttgttt agcagatccg ccagcagaat ccccttcgac
    12841 taaatacaat tcatttttct tagggtttcg ggattgtgcg ggtgtcaatt ttcctgaaag
    12901 aagagattcg ccttttttac gcttcttccc atttcggctt tcttctctgg ctttacgtgc
    12961 tgcttctctc gcttctctgg ctttgattgc tttacggacc aacatctggc tcatttcgct
    13021 attttcctgc aaataaaagc ccatttgttc actgatgacg ttatctacta cggtacgagc
    13081 aactggcgta ccaagttttt cttttgtttg cccttcaaat tgcagaagat tttcaggtac
    13141 acggatagaa agtacagcag ctaacccttc tctgaaatca ctaccttcca gattcttgtc
    13201 ccgttctttc agcaaaccaa cttttctcgc atattcatta taagatttcg tcatcgccgc
    13261 tttcatacca gcttcatggg ttcccccatc ttttgtccga acgttattga caaaagataa
    13321 aacattttct gaataaccgt cattgtattg ataagcgacc tctacttcga taccttcttt
    13381 ttctcctgaa aaataaacaa ctggtgtcaa tgtatctttt tcttcattca gataatcaac
    13441 aaattctttg attccttctt cgtaatgaaa aatttccttg actggttctt ctccacgaag
    13501 atcagaaaga gagattttaa cgccttttag aagaaaggca gattctctta accgttcggc
    13561 taaaatttca tatgaaaatt tcgttgtact gaatattgta tcatctggta aaaaatggac
    13621 agtcgtcccg tttgcttttt ttgtttttcc tatctttttc aaagtaccgt caggtttacc
    13681 gccgttttta aatttctgct gatactctac gccatctcta acgatcgtta caattagcca
    13741 tttcgataag gcatttacca cactggctcc tacaccgtgc agtccaccag aagttttgta
    13801 ccctccttga ccaaactttc cgccagcatg cagcacagta aaaatcactt caactgttgg
    13861 tattccggaa gcatgcattc ctacaggcat cccacgtccg ctatctgcaa cagttatact
    13921 attgtcttca tggatggtca catcaatctc actgccataa cctgataatg cttcgtcaac
    13981 tgcattatcg acgatttcat agactaaatg atgcaagcct cggctgtcag tggatccaat
    14041 gtacatccct ggtctttttc taactgcctc taatccttcc aatacttgga tggacgagtc
    14101 attgtattcg ttgtttactt tttttgccaa ggcaaacgct ccttcaataa tactttgcgg
    14161 caaaaccgca ccacgattat catactagaa aaggtcgtga aaaaaaagag ggtagacgag
    14221 aaatcaattt taattatctc ctaaccgctt tttcgacatt cccctctatc gatcatctct
    14281 tctttgtggt catttttatt tttggttttc tttcaactta ccaagttcaa tcttaataca
    14341 acgatccatt acgatatctg tacgtcctgc ttttcttagc atctcagctg cttctttgtt
    14401 ttcaatccct aattgtgccc agaacacttt agcatcagtt ttcaaaaaat cacctgctat
    14461 ttcaggtaaa aattcactcc gtctaaatac atctacgata tcgattggat catcgatttc
    14521 tgtcaaattc gcatagactt tctcgcctag aatttcttca ccagccaata atggatttac
    14581 tggtatgatt ttatatccat tgtcttgcaa tacctgtgca accatgtaac ttgttcgttc
    14641 tggtttattg ctcaagccta cgacggcaat attttttgct tcttccaaat agttgaaaat
    14701 cgtttcttgt gttggatttt ttacagacat ttctgccacc ccttgctttt ctttttattc
    14761 catctattat acttgtatct ttcatttttt caattgttcc tgttacctat tcatgctaaa
    14821 ataaaggata gttttcaact tgaaagaggg taaaaaatga aattgatctt attacttatt
    14881 ttagcatatc ttttaggttc tattccttcc ggtgtctgga ttggcaaatt gtttttcaaa
    14941 aaagatatcc gacaacacgg gagcggtaat actggaacga ccaatacatt tcgtgtatta
    15001 ggcaaaacag ccgggattgt tgtcctgctt atggatattt taaaaggtac attggctact
    15061 tgtttgccga tgatcttcca cctttctggg attaaccctc tttggtttgg ggtatgcgcc
    15121 attcttggcc atacatttcc gatttttgct gggttcaaag gaggaaaagc tgttgcgaca
    15181 agtgcaggaa tgcttttagg atttaatcct gtattttttg tttattcttg tatcatcttt
    15241 atcgttagtt tattttgtac aagtatggtg agtttgagca gtatgatcag tgcagtattg
    15301 atcactttat ctacgatcgt ccttccttat gtagctcctg tgattttggc taagcccaat
    15361 tggttactga cgatcattgc ctttttagtt tcttccttta tcttttatcg gcataaagac
    15421 aatatcaagc gtatcagaaa ccatactgaa agtcgaatcc cgtttggttt gaatagcagc
    15481 aagaaaaaat cctgatactt tttcattcca aagagataaa acgggagtgt gacaaatcga
    15541 tgtcacactc ccttattact tacaggatag cttttttaca caccttggta aacggtgttc
    15601 cctgctaact tctcggtcac aacctcttac ttcactgtga tggaatattt tgtcttgaag
    15661 atttcatggg cacctagttg attgatccct actttttcca tcaagtttcc actactgttc
    15721 accgtgtcag cgattccgca ccacggttcg atacaaacga aaggcgcttc ttttggataa
    15781 ggagaccaga tccctacata aggcatatct ttatagctta aagttacgct atgggaagat
    15841 ttttcacttc gaatggcgta cgcatttaga ccccttgttt caaaaaccaa ggcatcttgc
    15901 tcaaaaagtt cacgattcaa agccagattc gtatttgtct gacctaacgt cttttgatcc
    15961 atatcgatga atgggccttt caatggtagg cgtaatcttg atttcaatgg agaaaatgcc
    16021 agatagaaat cttcaaatga caatccttct tcaagaggta cattgaaagc aggatgtcca
    16081 ccgatagaaa agaacatttc tttttcaccg gtatttgcca cttgataatg aaccgtgatg
    16141 ccgtctcctc ctaattcata gctgatcact aattcaaaat caaatggata cttttctaat
    16201 gtctccggcg tactccttaa gatgaaacta gccatttctt gtccatgttc aatgacttca
    16261 aaatccatat cccgagcaaa tccgtgttgc cccatttgat atgttttccc ttgataaaca
    16321 tactgatcat cttttaaacg tccaacaaaa gggaataaaa ccggtgcatg tctgccccaa
    16381 tacgctggat caccttgcca gatatactct aaaccatgtt cttttgattt cagactgatc
    16441 aattccgcac cgtgttcagc gatttcggcg atcagctggt cattttgaat aataacagtc
    16501 attgccattc ctccaacttc gacttacttt ttaaaatggg aaggctggaa caaacatttc
    16561 tatctgcccc agtcccctta cttctaataa ctgtgttttg ctaactcgtc tttaaaacgg
    16621 gaattcaata cttttaaata agttcccttc atgcctaacg aacgagattc aatgattcca
    16681 gctgattcca attttcgtaa agcattgacg atcacagaac gagtgatgcc gatactatcg
    16741 gcaatattag aagcagtcag acgaccttca tctccatcca atgcttcaaa gatagcttgt
    16801 acagccttca attcgctata agataatgtg ttgattgcca tttggacagc cgttgcactc
    16861 cgtacatctg cttcgatgct tcgagacttc tgataaagga tctgcatacc gacaaccgta
    16921 gctccatatt ctgctaagac tagatcgtca tctgcaaacg attgttccat acgagataag
    16981 ataatcgtac ccaaacgttc gccagctcca aagatcggaa cgacagtcgt cagaccaaat
    17041 ggatatttct ctcttgactc taccggaaat gcagtaatat cactagtaat tgggatatta
    17101 gcttcagttt tcatcaactg tgccactgta tcagtataag attcaggaaa ttgtttctca
    17161 acaaacattg ttttgattcg ttcattgttc acatcgtgac gttcattata acctagtaat
    17221 actccgtcac ttcctatgat ataggtgttg ctgtcaagaa taccacctag tgtgacagcc
    17281 attttatcgt aaggcaaatc tgcttgcatg tcaaaagtat tcttttgctg caaaagtgtg
    17341 tttatcttgc gggttttcgt tagcaagtct gtcattgtcg tcctccgatt ttttataaaa
    17401 tatagcgact taaatcttca tttgccgcaa ttgagccaag tttgtcatcc acatatgctt
    17461 ctgtgatcgt tatttctccc atttgcatat ctggtgcttc aaataataga tcttctagta
    17521 gtttttccaa aatggtatgc agtctgcgtg cgccgatatt atctgtgtca cggttgacat
    17581 cgaaagcgat ttgagcgatc cgttcaattg cttcttttgt aaacgtaact gacacatttt
    17641 ctgtaccgat caacgcgacg tattgtttta ctaaagcatt atttggttcg gtcagaatct
    17701 tcacaaaatc ttcagctgtc aaatcatcta attctacccg tatcgggaag cgtccttgaa
    17761 gttctggaat tagatcactt ggtttagaca aatggaaagc tcctgaagca atgaatagga
    17821 cgtgatctgt ttggatgatc ccgtattttg tgtttacttg tgaaccttcg acaattggca
    17881 atatgtctct ttggacgcct tctctggata cttctccaga ttgctgtgat ttagaagtga
    17941 tcttatcgat ttcatcgata aaaataattc cactttcttc cgctaatttg attgcctcac
    18001 tatggatatc agcatccttg actaattttg ctgattcttc cttgatcaat aactctaaag
    18061 cttctttgac tgttaccgtc cgttccactt ttttcttcgg tgtcaaagca cctagagttt
    18121 catttaggtc gatccccatt tgctccaatc cattgttcat cattggtgca ggtttctttt
    18181 ggtcttcaac ttcaatggtt acttcacgac tatccagtaa acccttttgt agttgttcca
    18241 agactgtacg gcgattgatt cgaatatctt ctgtcacttc ttcttgcggt tcagcggtat
    18301 tttgctgact agcattaaaa agattcatca tttgttcaaa ttgattagtg gattgtttct
    18361 gttctttttt gataccaggt accaagattt ttaccaaacg tttttctgca ttttttttag
    18421 cacttgtgta tacacggctg tactgctctt tttctacgat ttggatcgca ttttctacta
    18481 aatcccgaac cattgattct acatctcttc cgacataacc gacttcagta aattttgttg
    18541 cttcgacttt gacaaatggt gcattgacga tttttgccaa acgtcgtgcg atttctgttt
    18601 taccgacccc tgtaggtccg atcatcagca tattttttgg tgtaatatct tgttgcattt
    18661 tttcatctaa ctgcaaacgg cgatagcggt tacgcaaagc aacggcaaca gatttttttg
    18721 ctgcgtgctg tcctacgata tattcatcta attcacttac gatttgtctt ggtgtttgtt
    18781 ttaattcata catatctact cacctcttac agttcttcta cgataatatt atgattagta
    18841 aagatacaga tatctgcggc aatatttaac gcattttctg caatttcttt cgcactcatg
    18901 tcgttttgag catagttttt cattgctcgg gctgcagcca acgcaaaatt ccctcctgat
    18961 ccgatagcta agataccatc atctggtgtg ataacttctc ctgtacctga aacaagaagc
    19021 atttctttat cattcattac gatcagcatc gcttcaagtt tttgcataga ttgctgtgtc
    19081 cgccattctt gagctaattc gacagctgcg cgttgtagat tcccgttata ttcgtttagt
    19141 ttgccttcaa atttttcttc taaagtaaac gcatctgcaa cacttccggc gaaacctaca
    19201 acaacttctc cattgtagat acgtcgtact ttctttgcag tgcctttcat gacaacagat
    19261 tcacccattg ttacttggcc atctccagcc atagcaaatt tcccgtcttt ttcgacggca
    19321 cagatagtgg ttgaatgaaa tggtgattcg accatgtttg tttcctccta aatttgttct
    19381 tttccttatt tttacgctct tggatggaaa gaacgatagt ttttttgtaa actttctttt
    19441 gttacatggg catagatctg ggtagtcgat aaattggcat gtcctagcaa ttcttggact
    19501 gtccgcaaat ctgccccatt atttaataaa tgagtagcaa atgtatggcg cagcatgtga
    19561 ggatggatat cactatccaa actgcttttt ttgatcattt gattcaagac atattcaatt
    19621 ccagtaggcg tgattggttt tccatgatga ttcacaaaaa cgatatcatg ctcttcgtga
    19681 tattttgtca tcaatacctg gcggccttcc ctgaaatatt cttgcagagc atcttgagca
    19741 aaagaaccta gaggagcata tctctctttg ttccctttac catggatcaa catgacctct
    19801 gaatcaaaat caatgtctga taccgacaaa ctgctacact cacttaaacg gatgcctgac
    19861 ccgtaaagga cttccagtaa agcgcggttc ctcaaatcca aaggactatc tcctgctacg
    19921 ctgtcaaaaa gtgcttgcat ttcattttca taaaaaaaac gtggcagtct caaatttttc
    19981 tttttgagat gaacgtaaga aaatggattt tcttttatca cttcttcttt gagaagatac
    20041 tgataaaacg aacgtaggct ggctatcttg cgactgatag aatttctgct atagctagga
    20101 cgatgatcag ctaatgcact caaatagaca cgaaccgtaa aatattccgc cttcagcaga
    20161 tcagcgtctc cagattcctc caaaaaatgt ttgaaatgga gaatgtcttc ttcatatgct
    20221 tctttcgttt tttctgaata tcctctttcg acaatcaaat aacgcaaaaa tttttgttcc
    20281 cacgtggctt ctgtcatttt ctcaccttcc ttttgtccca gagaataatt tagcacagcg
    20341 tttataaaaa gacaaataaa ttgtcagaat ttaaccaata tttctaagtt tgttcacaat
    20401 tggcgcaatt cctcacattt tgtttgagaa attcttaaga aaaacgcaag aaaaagagcg
    20461 agactatccg tctcaccctc ttcatggagt ttccttactc atttgggatt gcagtttttt
    20521 ctaattctgt ttttattgcc tcattagctt ttaaagctcg ttgtgcaatt gcttcatatc
    20581 ttgctttttt gtctcgaatg cgctctggca gttcaggaaa cagaccatag ttggcattca
    20641 tcggttggaa atgttttcct tccgcatgag taatgtagta agccatactt ccaatagctg
    20701 tttcttgtgg aaactcgact ggttccattt cttttgctaa gcgtgcggcg ttgattccag
    20761 caagtagtcc gctcgctgca ctttcaacat agccttctac accagtcatc tgacctgcaa
    20821 agaacagatc ttcgcgtttc gttgattggt aagtcggttt cagcaattct ggcgaattca
    20881 taaagctgtt tcggtgcatc acaccatagc gaacgaattc agcattttct aacccaggaa
    20941 tcatccggaa aacacgtttt tgctcgcccc atttcaagtg agtctgaaag cccacgatgt
    21001 tgtataaaga agctgcagca ttgtcttgac gcagctggat aacagcataa ggacgtttgc
    21061 cagttttagg atcttccaat ccaactggtt tcataggtcc gaaaagcatc gttttgaatc
    21121 ctcgctgtgc cattacttca attggcatac atccttcaaa atatttttct ttctcaaaag
    21181 atttaagttc tgccacttcg gcattgatta gtgcctcatg gaaagctttg aattcctctt
    21241 ctgtcatcgg acagttcaag taagctgctt ctcctttgtc atagcgggac ttcaaatata
    21301 ctttatccat atcgatcgta gctttgtcta cgattggtgc agctgcatca tagaaataaa
    21361 aaccatctga accattgaat gctttgatgc tttctgctaa tggatgagaa gtcaaaggtc
    21421 ctgttgctac tacggtgatt ccttccggta tttcagtaat ttcttcgttt atcactgtta
    21481 ctaatggatg ctctttgact tttcgggtaa ttgtttcaga aaaagaatcg cggtcaacag
    21541 ctaaggcacc accagctggc actgctgttt catctgccga agtgattact accgaattca
    21601 atcggcgcat ttcttctttc aataacccta ctgcattagc caaactgttt cctcttaatg
    21661 aattggaaca tactagttca gcaaaattac ttgtgtgatg cgcctcagtc gatttgactg
    21721 gacgcatttc atataaacgg acagggactc ctgcttgtgc gatctgccaa gcagcttcac
    21781 ttccggcaag ccctgctcca acaacattta ccattttcaa tgaaagaacc tcttttctat
    21841 tatttttgga tgtcttcttc gtaatctcca tttacacaga ctacttgttt accgcctttg
    21901 actttcttct cgaccagata tccgtcacat tttggacaat ttctgcccac tggtttatcc
    21961 caagaagtga attcacattc tgggaaacgg gaacaaccat agaatagtct gtttttctta
    22021 gattttcgtt caatgacttg tccttcatga catactgggc aagtcacacc gatttctttt
    22081 acgattggtt tcgtattccg acaatcagga aaattactgc aagcataaaa cttcccatat
    22141 ttccctaatt ttatcaccat cggatgtcca cagacatcac aatcgaatcc tgctggttca
    22201 tctttgattt gaattttttc gattttttct tccgcattcg tcagttcttt ttcaaacggt
    22261 ttatagaaac gatcaaccac tttgacccac gcttccgtgc cttcttcaat tttatccaaa
    22321 tctccttcca tttctgctgt gaaatggata tcgacgattt gagggaagaa ttcacagatt
    22381 aatgaattga caatttctcc tagttcagtc ggctcaaatc ttttttgtgt caattttaca
    22441 tagtagcgtc tttggatcgt atcaagtgtt ggtgcataag tggaaggtcg ccctaccccg
    22501 ttttcttcta gtgcacgaat caacgtagcc tcactaaaac gtgctggtgg ttgagtaaaa
    22561 tgttgttttg gttcaatatc aacagattgg actacatcgc cttcttcaag ttctggaaga
    22621 atattttctt tctcttcttt gccgtcatct ctaccttcca catacacttg catgaatcct
    22681 ttgaatttga tttttgaacc gttcgcaatg aagatgacac cattttgttc taatgtcact
    22741 ttcattgtat ctaaaatagc tggtgtcatc tgactagcca caaaacgtga ccagatcaat
    22801 gtataaagtt tcaattgatc tttatctaaa tattgtttga gttcatctgg tgtacgaagc
    22861 acactcgttg gacgcacagc ttcatgggca tcttgtgcgc cttgagcatt tttgatcttt
    22921 cggtgatcat gtctagaaaa ttcttttcca tacgtatctt caataaatgc tgcggcctct
    22981 gcttttgctg tatcggcaat ccgggtagaa tcagtacgca tgtatgtaat caagccgact
    23041 gttccttgtt tacccaaagt gattccttca tataattgct gtgctaccat catggttttt
    23101 cttgttcgaa agtttaactt acgggcagct tcttgttgca ggctacttgt agtgaaagga
    23161 ttcgcagggt ttcttttgcg ctctttcttt tctacttttt taacttcgaa gtctgttcca
    23221 tcaattctag cagtgacttc ttttacactt tgtgcatctg gtaatttctt tttcttaccg
    23281 tcgattcccc agaaatttgc tttgaatttc ttgcgagctt ttttaaaatt accgtcgatg
    23341 ctccaatatt cttctggaac aaattctcgg atctcttttt cacggtcaat aataattttt
    23401 aaagcgacag attggacacg tcctgcactt aaccctttct tgaccttacg ccagagaata
    23461 ggactgatcg aataaccaac gattctgtct aaggtacgac gagcttgctg tgcatctact
    23521 aaatctacat cgatcgttct tggttcctta aaagctgctt tgactgcgtc ttttgtgatt
    23581 tcattgaaga ccacccgatt tttttctttc agatccaacc caagtaggta cgctaaatgc
    23641 caagcaatcg cttctccttc tctatccggg tcacttgcca agtaaacttt ttgagctttt
    23701 tttgctgcgg ctttcaggct tttgatgaca tcgccttttc cgcgtataga aatataatgg
    23761 ggttcataat tgttttcaaa atcgataccc attttacttt tcggtaaatc tcttatatgg
    23821 ccaacactgg ccattacttt atagtttcgt ccaagatatt tctcaattgt cttggctttt
    23881 gcaggtgatt ctacaatcac taaatattta tacgccatta ctgaatggac tcctttccat
    23941 tgtcgtgttc ctactattaa aagaggattc atacaaatcg tagctaatca tatccattca
    24001 gaaaacgttt gtcaagaaaa gcaaatgaaa agtatggtgt tattctttaa attctgccaa
    24061 gatatcttca cccgaaaccg tgcaaactgc accgtcttgg atcaaatgat gacatccggt
    24121 agaatgttct gttaaaatat ttcctggaac agaaaaaact gttcttccaa gatccaatgc
    24181 ttgctgtgct gttatcagag agccgctttt tttcttggct tcaaagacac atgttccttt
    24241 agacaaagct gcgataatcc gatttctcat tggaaaacaa tgctttttcg ggccggattg
    24301 aggaggatat tcactcaaaa gaagatgatc atttgcaatc acctcttgca agtcacgatt
    24361 ttcagcagga tagacttgct ctatccctgt tccaatgact gcgattgtcc gtccaccatg
    24421 agagattgct gcttggtgac cgtatgtatc tatccctttc gcgcagccac tgacgatcac
    24481 atatccagct tctatcactg gttttagtaa ataataaact gtttttttgg ctaaagcaga
    24541 agtgtgtctt ccaccaacaa cgcttaacat gttacaagaa agtaaagata gatcaccatt
    24601 ataaaacagt acaattggcg gataggtcaa gtgaagcagc tgagggggat atttgggatc
    24661 aaagcaagtg ataaacgact gtttcgattg gattttgccc cagttagcgt gaatttcctg
    24721 ccaacttttg cgaaacagct cccggtgttt ggttatttta cctatcttaa tgatgtcttc
    24781 aaatccgatt tctttataag gatactgcat catccagtta gcaagtgccc attttccttg
    24841 actactgatt ccttttgtat aagcaagttt caataaattt tcttctattt gatacatcat
    24901 aaaaccgcct ttcgtctctc tttctaataa gatacgtaaa agcggctgtt ttttattgta
    24961 aatatttttt tacaggtgca aaacttttac gatggattgg cgtaattcct tgtcgttcaa
    25021 gtccttctaa gtgttccttt gttccataac ctgcattatg cgagaaacca tatccaggat
    25081 aaagcacgtc atattctttc atcaattggt ctcgatatac tttagcaatg atacttgcag
    25141 cagcaatcga cacagatcgt gcatcccctt tgattatttt ttcctgctga ataggcaaat
    25201 ccaaggtcat cgcatcgatc aacaaatagc ttggtacaat ggacaatttt tctactgctt
    25261 ggatcatcgc taatttactt gcttcataaa tattcacatc atcgattgtc tgctgatcaa
    25321 cgatcccgat cccgattgcc agggcttttt cttgaacttg ttgataaaga agttctcgct
    25381 ttttctctga taattgtttg gagtcattca gccctaatat gatctcattt ttcggcaaga
    25441 taactgcagc tgccacgaca ggtccagcta atggtcctct tcctacttca tcaatgccgg
    25501 caatccattg gtgaccttgt gcgtaccctt gcttttcaaa gcaaaacatt tcttcatatt
    25561 tttccttaat taatgcttcc tttttcaatc gtcgttcaaa agaagccagt gcttgctgca
    25621 cgcctttgcg tggatcattt cttaatgatt ctagtgtagg atcatctatt ctctgtatgc
    25681 ttttcaatcg ttctttgata ctttggatgg attcagtcat cacttttttg ctccatttct
    25741 tcgcaacgat ccagtgtgta tcgtccaagt tttccttgac gaatatcatg cacgactaat
    25801 tcgctgccac gatcatagtc ttctttatac ccacgttttg ctgtaatatc catcagtaat
    25861 tccggtgcca atttttcttc ttgctcgatt gataaaccat aacgatcgct gattcttcct
    25921 gaataatatc gggcaaaaaa gtctaatccg taaatcgcta aatcatctaa atggacaagc
    25981 tggtctttga tcgcgcctgt cagcgctagt tttttgccga tttcttcatc ttcaaatttg
    26041 ggccataaaa tccctggtgt gtctaacaat tccaactcat tacctaaacg aagccactgc
    26101 tgaccttttg ttacgccagg cttatttcct gtctgtgcaa ttttcttgcc tactagtcga
    26161 ttcatcaacg tagatttccc aacatttgga atacctaaga ccattgcccg aatagcgcga
    26221 ggcttgatcc cccgagcagc atctcttgcc cgtttttctt tcaatgcttc tttagccgca
    26281 ggtactagac ctttaattcc tttactttct tgtgccgtga tagctaatgc aagatggcct
    26341 tgtttacgaa aatattcttg ccacagcttt gtctgttgtg gatctgccag atccgcttta
    26401 ttcaataaaa caaggcgagg tttttgctga atgatctgat ctagcattgg attacgagaa
    26461 gataaaggta atcgtgcgtc taccaattca aatacgatgt ccacatattt cattttttca
    26521 gaaacttctc gtcgggcttt tgccatatgt cccggaaacc attggatggt cattgttctc
    26581 tccattcctt tcataccgga atattccccg gctttacttg tttttttctt tttcaatata
    26641 aattcacttt tttattataa agaaattcca tctattagtt aagtgaaagt gaaccagtcc
    26701 tcattctatc acatcttcac ttttttgatg atgcaaattt ttattttggt ttctatatat
    26761 gaatctaaat ttttgacagc gtttcctaaa taagagatga tagacgaaca aaacagaaag
    26821 gagacgaaac aaatgaatag tatggttttt gttaattttc ctgttgctga tgtcaatcgt
    26881 tccgttgctt tttatgaaaa gctgggtttt aaaaaaaatg aagaattttc tactgaagaa
    26941 gctggttcaa tggtatggga tgacactttt tggataatgc ttctctctca tgatttttat
    27001 aaaaaattta taaaagataa agaaatagct gataataaaa aaacaagtgg tgctttgatt
    27061 tcgtttagtg ttgaaaatgc ggatgaagta aaaaaatttg ctcaaattgc aaaagaaaat
    27121 ggtggtactt tccacgaagt agatatggga atgcctgaag aagaaatgtt ttcattagaa
    27181 gtccaagatc tagacggaaa tacattagaa cccgtatgga tgaagatgta agcagataac
    27241 taatctgacg ttttttataa caaaaaaaca aaggcggatg atacgataag tattctccgc
    27301 ctctattttt tttattcgta caatctcttt tttacagttt ataaaagcag tcttctcacc
    27361 ctttttttaa ttgttagatg ccagtgtatg taaatcacca atgtgtcatg acacattggt
    27421 gattttatgg tatcctttta ttattaactg acaaaggaga gaatattttg aacactttga
    27481 ttgcaaaaga cgtttttaga ttgcccgaaa tgaatgcaag attgttgtcg acctctgctc
    27541 ttttattagc ccttgatgtc atcttataca agatagcgat tggtcctgct tattttcaaa
    27601 tcacattcgg atttttaagt atggcgctta tgggttatct atatggaccg atctgggctg
    27661 gtttgcttga aatgttgtcg aacactttaa atttcacgat atttggttca ggaacgtttc
    27721 aagctgcttt tcttatcacg gctttccttg gtggatttat taatggctta tttttatatc
    27781 ataaaaagat aaacctgcct tccgtcatcg ctgcccaatt attgattatg atcccagtca
    27841 gcctttttat caattcttgg ctgatgtctg ttttgtttgg tgcagatttc aaagtaattt
    27901 ttctaggtag attcatcagg aatatgatcc aaatccctat tcaaataatg gttgtttatt
    27961 catttatcaa aactttagag gctagaaaag tgtttaaacg agctgtttga ttttacgtac
    28021 taaggtagta aactgatttt ggattaaaca ataaagcgtg cagtctcaac agactgcacg
    28081 ctttattgtt tctcactgga tccattttat ttcatcaagt ggataataga caaatcttgc
    28141 tttacctaga atatcagttt tattgatcgt gccaaacgaa cgactatctt ttgaaactct
    28201 tcgattatct ccaagtacaa aataattatc ttccggaagc ttggattgtc ccgttagctc
    28261 ttgtagtgta aagtccgtcg tataggatgc atttgcatga tctgatttca ggtttttagt
    28321 tagataaggt tccttaacta ctttcccatt gatcttcaat tgatcatttt ggtagctgac
    28381 attttctcct ggaagaccga ttactcgttt gatataaatc gtgccatctg ctaattgaaa
    28441 aacaacaata tcaaatcgtc tgatctccga aaatttttcc atcacaacca tatcaccttg
    28501 cttcaacacg ttttccattg aattgccttc cactggtacc gggatgaata aaaatccccg
    28561 caacatgaac gcaaggaata cagaaacaag taagtatttg aatacaagcc agaagcgatt
    28621 aatatagcgt tgtttttttg tcaatctct
//
LOCUS       DAYYGO010000020        27835 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000020 DAYYGO010000000
VERSION     DAYYGO010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.020"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(22..219)
                     /locus_tag="ACHA5T_000790"
     CDS             complement(22..219)
                     /locus_tag="ACHA5T_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFM8886388.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(613..2340)
                     /locus_tag="ACHA5T_000791"
     CDS             complement(613..2340)
                     /locus_tag="ACHA5T_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFM8886389.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2333..3526)
                     /locus_tag="ACHA5T_000792"
     CDS             complement(2333..3526)
                     /locus_tag="ACHA5T_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8886390.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3713..4357)
                     /locus_tag="ACHA5T_000793"
     CDS             complement(3713..4357)
                     /locus_tag="ACHA5T_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8886391.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4451..4585)
                     /locus_tag="ACHA5T_000794"
     CDS             complement(4451..4585)
                     /locus_tag="ACHA5T_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFM8886392.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4587..6719)
                     /gene="feoB"
                     /locus_tag="ACHA5T_000795"
     CDS             complement(4587..6719)
                     /gene="feoB"
                     /locus_tag="ACHA5T_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFM8886393.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6716..7189)
                     /locus_tag="ACHA5T_000796"
     CDS             complement(6716..7189)
                     /locus_tag="ACHA5T_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFM8886394.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7590..7814
                     /gene="nrdH"
                     /locus_tag="ACHA5T_000797"
     CDS             7590..7814
                     /gene="nrdH"
                     /locus_tag="ACHA5T_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFM8886395.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            7973..10132
                     /gene="nrdE"
                     /locus_tag="ACHA5T_000798"
     CDS             7973..10132
                     /gene="nrdE"
                     /locus_tag="ACHA5T_000798"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFM8886396.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10182..11147
                     /gene="nrdF"
                     /locus_tag="ACHA5T_000799"
     CDS             10182..11147
                     /gene="nrdF"
                     /locus_tag="ACHA5T_000799"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFM8886397.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11236..12375)
                     /locus_tag="ACHA5T_000800"
     CDS             complement(11236..12375)
                     /locus_tag="ACHA5T_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8886398.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12684..13397)
                     /locus_tag="ACHA5T_000801"
     CDS             complement(12684..13397)
                     /locus_tag="ACHA5T_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFM8886399.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13651..14352)
                     /gene="nagB"
                     /locus_tag="ACHA5T_000802"
     CDS             complement(13651..14352)
                     /gene="nagB"
                     /locus_tag="ACHA5T_000802"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFM8886400.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14586..16118
                     /locus_tag="ACHA5T_000803"
     CDS             14586..16118
                     /locus_tag="ACHA5T_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8886401.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16309..17511)
                     /locus_tag="ACHA5T_000804"
     CDS             complement(16309..17511)
                     /locus_tag="ACHA5T_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8886402.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(17593..18282)
                     /locus_tag="ACHA5T_000805"
     CDS             complement(17593..18282)
                     /locus_tag="ACHA5T_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM8886403.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(18388..18756)
                     /gene="rplL"
                     /locus_tag="ACHA5T_000806"
     CDS             complement(18388..18756)
                     /gene="rplL"
                     /locus_tag="ACHA5T_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFM8886404.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(18809..19312)
                     /gene="rplJ"
                     /locus_tag="ACHA5T_000807"
     CDS             complement(18809..19312)
                     /gene="rplJ"
                     /locus_tag="ACHA5T_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFM8886405.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(19516..19643)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(19722..20411)
                     /gene="rplA"
                     /locus_tag="ACHA5T_000808"
     CDS             complement(19722..20411)
                     /gene="rplA"
                     /locus_tag="ACHA5T_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFM8886406.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(20515..20937)
                     /gene="rplK"
                     /locus_tag="ACHA5T_000809"
     CDS             complement(20515..20937)
                     /gene="rplK"
                     /locus_tag="ACHA5T_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFM8886407.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            21280..21948
                     /gene="sdaAB"
                     /locus_tag="ACHA5T_000810"
     CDS             21280..21948
                     /gene="sdaAB"
                     /locus_tag="ACHA5T_000810"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFM8886408.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            21961..22833
                     /gene="sdaAA"
                     /locus_tag="ACHA5T_000811"
     CDS             21961..22833
                     /gene="sdaAA"
                     /locus_tag="ACHA5T_000811"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFM8886409.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(22924..24024)
                     /locus_tag="ACHA5T_000812"
                     /pseudo
     CDS             complement(22924..24024)
                     /locus_tag="ACHA5T_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(24116..25003)
                     /locus_tag="ACHA5T_000813"
     CDS             complement(24116..25003)
                     /locus_tag="ACHA5T_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8886410.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(25080..25625)
                     /gene="nusG"
                     /locus_tag="ACHA5T_000814"
     CDS             complement(25080..25625)
                     /gene="nusG"
                     /locus_tag="ACHA5T_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFM8886411.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(25726..25896)
                     /gene="secE"
                     /locus_tag="ACHA5T_000815"
     CDS             complement(25726..25896)
                     /gene="secE"
                     /locus_tag="ACHA5T_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFM8886412.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(25920..26072)
                     /gene="rpmG"
                     /locus_tag="ACHA5T_000816"
     CDS             complement(25920..26072)
                     /gene="rpmG"
                     /locus_tag="ACHA5T_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM8886413.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            26234..26878
                     /gene="cbpA"
                     /locus_tag="ACHA5T_000817"
     CDS             26234..26878
                     /gene="cbpA"
                     /locus_tag="ACHA5T_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFM8886414.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(26934..27437)
                     /locus_tag="ACHA5T_000818"
     CDS             complement(26934..27437)
                     /locus_tag="ACHA5T_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFM8886415.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(27450..27496)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
BASE COUNT         8414 a         5767 c         4943 g         8711 t
ORIGIN      
        1 tgcttagcat tttttttcat tttagcactt ccctggaatt gctccaccta aaaaaccacc
       61 tatagacatt cctgcaatac aagtagttgc cttggcccaa tcgaccgtac atttattttt
      121 agtgcaatac actccatttc cataatattt tccactatga gtggtacccc catagataag
      181 ttgtgtctct ttaatagaca aaatttttaa atgtttcata atctaatcac ccctaaatta
      241 atgtttttag actattattc taacattcat taaatttcaa aaaacaattc cttcttgaaa
      301 cgtagcaaaa taattcctga ataagaattt aatcactttt aagtatataa actatgtata
      361 ttctttaata ttttttgaga atgcacttcg tacataataa actttaatca cattagtctt
      421 tcatacctac gatcgatctc tttgactcgc acccataaat tcataatagt ttgtcggcgt
      481 ctttggacgt ttcgaagatg agctacgcat tctctttttt tatagacaaa aaagatagag
      541 acgaacatgg agcttccaaa tttggatatt tgatccaatt ttggaagctc catttatttc
      601 gtgtctgtct ttttattttt tccagctttc gactaattct tggttctctt tgatccagtc
      661 ttttgctgct tgctgtggat cttttccatt gttgatttcc aacataactt tttccatatc
      721 tttttctgac caatggaaat tgtctaatac tttgtaagct tctggctggt cttctttcaa
      781 tccttttcga accatcgtat ggatctgctc ttcgcttccc attccgtttt caggatcttc
      841 taagtatttc agatcatatt tggcaaacat ccaatgaggt gtccatcctg taactacaat
      901 tggttcgtgt tttttgatcg cttgacctag tgcaacggtc attgctcctg aagaagatgt
      961 ctctactttc cagtctttca agttgtcata tttttggatc gtgttttcag cagccgtaac
     1021 tacaccagct cctggctcga ttcctatgat tttcttacct gcctgatcgg ttaactcatc
     1081 gatcgaattt acatccatgt aggctggcac tgctaaacca acttttgctc ctgtcaaatt
     1141 ggcacctagg tcttcgactt gatctttata ttgtgcatat tgtgaaccat gagttttagg
     1201 tagccaagct gaaaccatgg catctgactc gccatttgaa acagatttcc acatgattga
     1261 attatctaat ggcgtcatcg ttacatcata gcccatttgt ttcaatactt caccgactac
     1321 atttgttgaa gcaacttctg tatcccattc aacataagat aagttgatac gttttgtttc
     1381 tttggcagat gaaaaactcc ctataccaat cgcacctaag atcactacta ctacagcacc
     1441 aatgatcagt ccttgacgtt ttttactttt ctgtgttgtt gctcctgcag cttttttatt
     1501 gtttggctgg ttcaaatgct gtgtaaaacg gtcaatgatg attgctaaga taactaatgc
     1561 aaccccgttg acaaatccat tcccgacttg tgctctttgc aatgctgata atacgccgcg
     1621 tcctaagccg ggtgcaccga tcatagaggc aataacgacc attgaaagtg acagcattgt
     1681 tgtttgattg atccctgcca tgatcgtact tttagctaaa ggcaattcta gtttgaatag
     1741 tttttgacga cttgtgctcc cgaatgaatc agaagcttct actaattctt taggaacttg
     1801 tcgaatccct aaattcgtaa aacgaacggt tggtggcaga gcaaagatga ctgaggcaaa
     1861 tacccctgga accatcccga taccgaaaaa tgcaactgct ggaatcaaat aaacgaatcc
     1921 tggcattgtc tgcataaagt caaggatcgg tttgataata ctttgggctt tactgctttt
     1981 tgccatcaag atccctaatg gtaccccaat gatgatcgag atcacactag ataaaagaac
     2041 aagtgtgaca gtattcatca aatcattcca tagaccttgg ttataaataa accatagacc
     2101 gattaatgtg aacaacgtaa gtcctggacg tttcttagaa ataaagaaag ctgctaccgt
     2161 caataacaag ataaacaaag gagctggaat cactaacagt aaatttgtaa tccccgacat
     2221 cactgcctgt ccaatcgttt gtaaaaatga aaataggcca gaaaaagttg tcgtaaacca
     2281 ttctgtgata ttttctaccc agtttgccac tggtaattga tagttcaata gattattcat
     2341 gttcactcac ctcgcttgtt tctgctaatg cttcaatcac actacctcgg atgacgacac
     2401 cttttaattt tccatcctct acaactgcaa gtggagtcgg tgagtcataa ataatattga
     2461 aaatatcact taccaccatg tctttcccaa tcaccgtcac attttcatct aggaagtcga
     2521 tcaatggacg tttttgcttc ctagcttcta acgcttgatc tgcagtgatg atccctttca
     2581 ggtgtctctt tctgtcaaca gccatcagca tgctgacttc ttcattacgc atccttgtca
     2641 atgcaacgtt tggtccatca gattcgatat tcgtcgtcaa tgcaggtacc atgatgtttt
     2701 gagccgtaag cactttggaa cgatcgactt cttccacaaa ttcacgtaca taatcatttg
     2761 ctggattggt caatatttct tcacctgttc cgatctgcat gatttcgccg tctttcatca
     2821 aggcgattct gtccccaatc cgtaaagctt cattcaaatc atgggtaatg aaaataatcg
     2881 ttttttgaac attggcttgc aggtccagta attcgtcttg catttctcgc cggatcagcg
     2941 gatcaagagc cgagaacgct tcgtccatca gcaagatctc aggatcattt gctaaagctc
     3001 tggctaggcc aacccgttgt tgcatcccac cagataactg actaggatat tggtctttaa
     3061 atgaaagcaa actagaattt tctaatgctt tttctgcttt ttcttgtcgt tcttctttag
     3121 gtacaccacg gacttctaac ccgtattctg tattttccaa gatcgttcga tgtggaaaca
     3181 gaccgaagtt ttggaaaacc atattgattt tatgacgacg gacttctcgc aattcttctt
     3241 tgcttaactt cgatacatct tctccatcaa tatagatact gcccgaagtt ggctcaatca
     3301 gacgattcaa tagacggatc aatgtggatt tcccactccc ggaaagcccc atgatcacga
     3361 agatttctcc ttcttttacg tcaaaattca cgtcatacac accaaccgtt gcaccggttt
     3421 tctttaaaat ctccgttttc ggctggtgat cattcatcat atctaaagcg gcttgtgtct
     3481 ttttaccaaa tatctttgtt aaatgtttta cttctacttt taccaaacaa attctccttt
     3541 tttatgtaaa ttcatttttc tttctgaaaa aagtgaccac actatgttaa catcatgtag
     3601 tcactttgtc aaaaatgaaa gaaatttgct tgtccaaact gaaaaaaact tggctaaatc
     3661 tttgtaaata aaaggtttag ccaagttaat atttttttaa tatttcacac aatcattctg
     3721 caagctgatg ctccgggtaa aaaaagtttg tcatacgctg tagtgtatat tcatttccaa
     3781 taaaatgaac gatgtcccct gccgtaaatt tagcataggg gccaggtgaa acaagcagtt
     3841 cttcattatg agaaattgcg ataactgtgg cgccagtcgc ctgccagata ttcaactcgc
     3901 taattgaacg gtccagatgc attgcttttt ctgataaaga taattcaaac ggaagtaaag
     3961 gattcatctt atgtattcgc ttcgtttgat tgaccagctg atccaataat tgagaaaaat
     4021 gattcagctc atcttgttgc ttctccacac ttgccatgat ttctgctttt gtatcttgga
     4081 tggattgtac atcttgatat tgcaatagga aatcttttgc tttttccttc gaagcaacaa
     4141 atgccccact tccatgccgt acttccatga ttccaagatc taccagcaca ttgatagcct
     4201 tgcgggtagt ctcaggagaa gcattgtacg tactggcaag tgttgagcgg gcatggatct
     4261 tctcacccac tttatatcgg ttttcaacga tacgttcggc taaatcaaca gcaatctgct
     4321 ggtagcgcgg gcggacgatt tttttctttc cagacatttt ctttcctctt tcttttttta
     4381 ctttccctca tcttactatg ctctagcaaa ataagcaaaa aaggggagaa tagcctcttg
     4441 caacgactga tcatcgcttg atctgttcct tttttactgg gcaagctgtt tgacagtcat
     4501 cacaattctt accggatttt aaacgagtat agacaattgc gcctgcactt ccaaagatta
     4561 acacacttaa taagaatgtc gccatcctat tcctctcctt ctaatgacaa tatagtataa
     4621 aactggttct tggattttgg ctgacggaac aaaacataaa cgagtcctat catcagtaaa
     4681 atagcaagat acgtccctaa cgctacctgt ccaccctcca ataagtgtcc caactgataa
     4741 atcacaaaac taactaaata agctaaaccg cattgatagc ctatggcgat ccaagtccat
     4801 ttcttggcat tcatttcccg atggatagcc ccaattgccg caaaacaagg cgcacaaagt
     4861 aaattgaagg ctaacaatga ataagctgct gcgggagtat atgtgtgttg tagcacttgc
     4921 cagatttctc tgccattttc ggatacttcc gcatgaccaa acaaaatacc aaatgtgcca
     4981 ataatatttt ctttagcgac caatcctgta atcgctgcga ctgttccttg ccagtttccc
     5041 caaccaagcg gctgaaaaac aactgccagg attcgcccga aaaaggctaa gatactttgg
     5101 tcttcagaaa caggttgagc ataaaaatta taagaagagc tgaaccagat cacgatacta
     5161 gaaacaaaga taatcgttcc tgctttttta acaaaagact tgctgcggtc ataggtttgt
     5221 cttaggaccg ctgaccattg aggaaaatga tatgcgggca attccatgat aaaaggagct
     5281 gtatctccag caaacaaacg tgtttttttc aagctgattc cagagaaaat aattgcagtc
     5341 ataccaagaa aataggcaga aggagctacc caagattgat gaggaaaaaa ggcccctgct
     5401 actaaggcaa taatcggcaa ctttgcagaa caaggcatga aggttgtgac catgatcgtc
     5461 aaacgacgat cttgctcgtt ttcaatcgtg cgactggcca tgacgcctgg cactccacat
     5521 cctgtagcaa tcagcatagg aatgaaggat tttcctgaaa gaccgaattt cctgaaaagt
     5581 cgatccatga caaaagcgat cctcgccata tatccgcagt cttccagaaa gctcaagcat
     5641 aaaaacaata taatcaattg cggtagaaat cctaacactg ctcccacgcc tgcgatgatg
     5701 ccgttcaaga tcagctcctg catccatccc gctacttgcc attcagctaa caatctgcca
     5761 acatgttctg gtaaccatgt accaaacaat gtgtcattga tccaatctgt ccccattgtt
     5821 cctactgttt gaatagccag ataatagatc agccacatca caaatgcgaa gatcggcaag
     5881 gctaaccaac gattcgttgc aatctgatcg attttgtcac tcatcgataa ttggaaagaa
     5941 tcatttgaca caacacagag tgtacatagt tgggtaataa attcataacg ttcattgacc
     6001 agaatcgtct cactatcgtc gtccaatagt ttctctgtta actggatcag ttgctcgatc
     6061 tctttttgct gagaaactgc tatctcgtaa tcctctctta ccttctgatc tcgttcaaaa
     6121 tatttcattg cataccagcg tttcttgccg ctttgttctc ctagtacatc ttcaatttca
     6181 tgtaaagctg cttctaaacg tggatcatat gtacagggaa caggaataga aggccgcttt
     6241 tttgcagact cctgaatctt ttcgattagt ttttcgattc cttgcttttt caatacgctg
     6301 attggtaaaa ctggtacacc aagaccatag gaaagctttt ctagattgat tttttttcct
     6361 tcttttttga tgacatccat catattcaga ccaatgatca taggaatgtc taaatccaat
     6421 aactgcgtag ttaaataaag atttcgttct aaattggtag catcaataat gttgatgatc
     6481 agttcaggct gttcctgaac caaataatct cttgcaatca cttcttctgg tgtataagga
     6541 gacaaagaat agatgccggg taggtcctga atatgaatgt tcggattttt tttcaacgat
     6601 ccttcttttt tttctactgt tacgcccggc caattcccta catattgatt cgaaccagtc
     6661 aagagattga acaagctagt tttcccgctg tttggatttc cgactaacgc gatattcatt
     6721 ctttttcctc cctttgtatc aagattagtt cggcctctga ctttcgtaac gtcagttcat
     6781 accctcgcac tgtcagttcg attggatctc ccagaggggc tagttttcgg ataactattt
     6841 ttacatttcg cgtcaatccc atatccatta accgtcgctt cactgcgcct tgtccgtgga
     6901 ttgctacgac gatcccagtc tctcctgctt ttaattgatc aagagaagtc cagctttcag
     6961 aggaagcagt ctttttattg acaaagattt gacctagtat ttcccgactt actgccactc
     7021 gatgactttt taccagtaaa atggcattgt tgcctgataa attgacgacg acgattttac
     7081 ttccggcaac gatcccgata ttttgcaaat ggcgttttgt tttttcttct gtttcgattc
     7141 gttcgacttg ataaacgaaa ttctgctttg cctgcgctaa ggtctgcata ttgattcctc
     7201 tcttgtgaaa taaatcatgt attaaaatca ttcattatat gttctaattt tcttacgtgt
     7261 tacgcaaata aatgagaatc attctcacat agaatactct cggtaattga gaatgtcaat
     7321 tatacttgtg atattatgag cgaatcttta gtattgatag ataaaaatga gaaaaaaata
     7381 tttcttcata gttttccaca attttttctc tattaaattt gctattttcc cacacgatac
     7441 ttgttttcag ctcttttttc aaaaacacaa aacttagtat tccacagttt ccacaacaca
     7501 atatgtagtg tttgttgttt gactttttta ctaagagttt tatactatct attatagata
     7561 ataagaatgt gttagaggag gcactcacca tgaacgtaaa aatcttttct aaaaataatt
     7621 gtatgcaatg taagatggtc aaacggtttt taacagaaaa tcaaattgct tttgaagaag
     7681 tcaatatcga tgaacagcct gaagcagtag actggttgaa agcacaagga ttccaaagtg
     7741 ttcctgtcat tacttctgat gcagaaacag ttatcggatt ccgtcctgat cagttgcgta
     7801 aacttgccag ctaacatgac taaaagaatc aaaacgtgat acatggattt gaagggcttg
     7861 agacagcacc agtttttgtc gcaaacttcc aaatcctttt ttaaagctta aaaacaatgc
     7921 aatgatttgc aatcaagcat catcaaatac gaaatgaagg ttgggcgtac gtatgagtct
     7981 taaaaattta caagatgtca gttatttcaa gctgaataat gaaatcaatc ggccagtcaa
     8041 tggtcaaatc cctttgaata aggataaaga agctttagtt gccttttttg aggaaaatgt
     8101 caaaccaaat acgatgactt ttctatctat tatggaaaaa atcaattact tgatcgatca
     8161 agaatatata gaagcagaat tcattcaatt gtattcacca tcatttattg aaaagctata
     8221 tcaatttttg gacgagcaac atttttcttt caaatctttt atggctgctt acaaatttta
     8281 ttctcaatat gcgttgaaaa ccaacgacgg ttccgcttat ctggaaactt atgaagatcg
     8341 agtagccttc aatgcgcttt actttgcgaa cggggatgaa gaattagcac ttgctcttgc
     8401 agacgaaatg atccatcaac gatatcagcc agctacacct tctttcttga atgctggacg
     8461 taaacgtcgg ggcgaattgg tgtcttgttt cttgactcaa gtcactgacg atatgaactc
     8521 tatcgggcgc tcaatcaact ctgctttgca attgtcccgt attggtggcg gcgtagggat
     8581 tactttatca aatcttcgtg aagccggcgc tcctatcaaa ggatatgagg gtgctgcaag
     8641 tggtgtcgtt ccagtcatga aattattcga agatagtttc agctattcta atcaattagg
     8701 tcagcgtcaa ggagctggcg tggtttattt gaacgttttc catccagaca ttattgcttt
     8761 cctttcagca aaaaaagaaa atgcagatga aaagattcgc gtaaaaactc tttctcttgg
     8821 cgtgatcgta cctgataaat tttatgaatt gacacgtaaa aacgaagata tgtatttatt
     8881 tagtccatat agtgtcgaaa aagaatatgg tgtcccattc tcttatgtaa atattacagc
     8941 tgagtatgat catctagttg caaatccaaa cattcgcaaa caaaaaatca aagcgcgtga
     9001 tcttgaaaat gaaatctcaa aattacaaca agaatctggc tatccttacg taatcaatat
     9061 tgatacagcg aaccgcgaga atccaatcga tggcaagatc atcatgagta atttatgttc
     9121 agagatttta caagttcaaa cgccttctgt gattaacggc aaacaggaat atgaagtatt
     9181 aggaacggat atctcatgta acttaggctc gacaaatatc gtgaatttga tgcaaagccc
     9241 taactttggt caatctgtgc gtacgatgac tcgtgcattg acatttatta cagatgcatc
     9301 tgatatcgat gtcgtaccac cgatccaaaa cggaaataag ctgaatcata cgattggatt
     9361 aggtgccatg ggcttacaca cttatcttgc aaaagaacaa atggaatatg gctcagagga
     9421 ttcattagac tttacaaata tttacttcat gctgttgaat tattggacac ttgttgaaag
     9481 caacaatatc gctcgtgaaa gaaaacaagt tttccataat tttgaaaaat cagcttatgc
     9541 tgacggcact tatttcgaaa aatacgtaac tggagaattc cagccaaaat cagaaaaagt
     9601 caaagcattg tttgaaggca tttttatccc aactgctgaa gattggaatg ctttgaaaca
     9661 agcagtaatg aaagatggac tgtatcatca aaatcgcttg gcagtagcac caaatggctc
     9721 aatttcatat atcaacgata cgagtgcaag tattcatcct atcactcgca tgatcgaaga
     9781 acgccaagag aagaaaatcg gtaaaattta ttacccagcg ccttatctat ccaatgagac
     9841 gattccgtat tacacttctg cttatgatat ggacatgcgt aaagtaatcg atgtttatgc
     9901 aatggcacaa caacacgtcg atcaaggcat gagtttgact ttattcatgc gttctgaaat
     9961 cccagaaggc atttatgaat ggaagacaac aacgaagcaa acgacacgtg atttgaatat
    10021 tttaagacat tacgctttcc acaaaggaat caaatccatt tattacgtac gtacctttac
    10081 tgatgatgca gaagaaatcg gaagcaatca atgtgaaagc tgcgtgatct aatatcatag
    10141 atagattcgc ttattcatat tttaattaaa ggagcattct catgtcagaa acatattatg
    10201 aagcaataaa ctggaatgaa attgaagata tcattgataa atccacttgg gagaagctga
    10261 cagagcaatt ttggctagat acacgtatcc cactttcaaa tgacctagat gattggcgta
    10321 cattgtctca attggaaaaa gatacagtag gacatgtatt tggcggtttg acactgctag
    10381 atactgtcca atctgaaagt ggcatggatc agttgcgtaa agatgtccgc actccccatg
    10441 aagaagccgt tttgaacaat attcaattca tggaatccgt tcatgccaaa agttattctt
    10501 ctatttttag tacactgaat acgaaaaaag aaatcgaaga aatttttgac tggacaaata
    10561 cgaatcctta tctgcaaaaa aaagctgagc gtatcaatga aatctacaaa cacggtacac
    10621 cgctagaaaa gaaaattgcc agcgtctttt tagaaacatt cttgttctat tcaggttttt
    10681 atactcctct ttattattta ggaaacaaca aattggctaa cgtagcagag atcatcaaac
    10741 taatcatccg agatgaatct gtacatggaa cctacatcgg ttacaaattc caattaggat
    10801 tcaacgagtt acctgaagca gaacaagaaa cgttaaaaga ctggatgtac aatcttcttt
    10861 atgagcttta cgaaaacgaa gaacgttata cagaagaatt atacgatcca atcggctgga
    10921 cagaagaagt caagacattc ctacgctata atgccaacaa agcattgatg aacctaggga
    10981 tggatccact ctttccagat actgccaatg atgtcaaccc aatcgtaatg aacgggattt
    11041 caacaggcac aagcaaccat gacttcttct cacaagtcgg aaacggctat ttgttaggtc
    11101 atgtagaagc gatgaaagac gacgattatt tgattggatt ggaataagga tttattttaa
    11161 ttcaaaaatt tttcgaaaaa taaaaagagt ttagcgtaca gatattttaa atgtacactg
    11221 aactcttttt tatgcttatt cacttgtttc ttccagcact tttggactgt ctaacttttc
    11281 cgaaacatgt tccattcttc tcaataaata gattttccgg atatttaata ttatcgtctt
    11341 aatcactgca tagacaggta tgcaaatcag catgcctaat attcctgcga cacttcctgc
    11401 tccaatcaat accaagatga tcgtcaaagg atggatattc attgatttcc caaataacaa
    11461 cggcttgatc aaatttccat ctaattgttg gatcactaaa atcgacaacg ccatatacaa
    11521 agcttgaaat ggagagataa acaaaccgac aatgattgct ggtaccgctc caataaaagg
    11581 gccgacatac gggataatat tcgtgattcc gcaaatgacc gcaagcaaca agctataagg
    11641 ctgatcgaat attctcattg ccaaaaagct cattacacca ataatcaaag catctaagat
    11701 cgtactgctg atataggcag ataatgtttc gtttaattct tttaccgttt ggcgtaactc
    11761 tttacgaatc gctttcggaa agaagtgaga caacgcatca ataaatttgt gcccatcctt
    11821 gaacataaat agcaaaataa atggaacggt aaataacaaa ataaaaaatt gcatcataaa
    11881 tgaaatgatt tttgagacac ttgcagataa actgactaat atgatattga acaaattact
    11941 aagagataaa tttgctgatg ctaattgttc ttccaaattg aaatttttaa actcttcccg
    12001 ctgcgccaat tcatttaacc atttgcttgt ttcatctgca tacattggca agctctgggt
    12061 caattgaatt gtctgttcca ttaactgagg cactacattc atgacagtta aaacaactaa
    12121 aatgattacg accaaaaacg tcaataaaaa accgaacact ctcgggatcc gatgcttttc
    12181 gagaaaaaca acgattggat cgaacatata atagataaaa ccagcaacaa gcaatggtaa
    12241 aaaaactgcc gaaagcattt tcaatattgg actaaaaata ttcggcattt gtaaaataaa
    12301 gtacacgccg atgatcgtga gtactagctc cactgtccag aaaaataatt ttgattgttt
    12361 aaaacgtgat tgcatacatc tcctccaagg tcctttgata cttctattct atcatttaac
    12421 aaagagttac gcgctgagat tttcatttgc ttaaaaaaaa caaaaaaaat aaacatattt
    12481 taatgagtag acgagtactt aaacaaaaac acggtttgga gtagtgacaa aaattatact
    12541 gcagtccctt tttccaaata aacggtgttc aaaagcttgc atcttcgtaa taagcccagc
    12601 caaacgaaaa atataaggaa ctatttttat ttggctgggc ttattacttg atgtaggaca
    12661 tctattttac aacctcagat catttattgc gcaatactga ctaaatgcaa ctttgtgata
    12721 tttttgtgta cggacaactc ctcgatcaca tcggcataag tgagcccttt tggaagatca
    12781 atcgtataga tatttttgta cactcggtag tcatcaacga attttacgtc aaaattcaca
    12841 tcttcgatta cgatattttt ctcttcaaaa taatcggtaa gaaaatcttt ggtttctttt
    12901 ctatggataa attggatttc taacttttta gtcgtaggta catggatcac gcgtttcact
    12961 actgtgagtg caatagcaat cccgataaaa ctgatgatcg caatggaata gtaacccatg
    13021 ccgatggcga tccctaatcc agcaattgcc cataaagaag cggcagtcgt aagtcccgtc
    13081 accgattgtt tcgtgacgat gatcgttccc gcgcctaaga aaccgacacc actcaccact
    13141 tgagcaatca gccttgcttc atctgaacga atcgttccag caagctgtgg atactctaaa
    13201 gcatctctta atgcaccact agcgatctca acttggatca atgcaatgat acatgctccc
    13261 atacaaacta aaatatgcgt acgcatacct gctggacgat ttttatattg tcgttcaaaa
    13321 ccgattgctc cgccaaatag catagcgatc cctaaacgta aaataatctc tggaattgtc
    13381 aataccatat cattcatcta aacaccctcc agtccaatat atcatgtctg aaaggagcag
    13441 acaaaaaaag tgcaattttt acctcttacg aaaaaaggac tgtgacaaaa tttttgtcat
    13501 agtccttttt atgaataaac ggtgttcaat cagctgaccc tcggtattac tcaacaaacg
    13561 gcaaaatatg gggagctatt tttcctttgt cttctctaat actcaggtca aaacgctgat
    13621 ttcacaacct ctttagccta ctattgaaaa ttacaactta cttgctgctt cttcatcgat
    13681 gatcacaaca acattgtcgt gattttgcaa tacacttgct ggcaaatgat tcgttacagg
    13741 tccttcaatc atccctttga ttgcatcagc tttgctttcg ccaaatgcca tcaagacgat
    13801 ttctttactt ttcataattg aaccgattcc cattgaaaca gcttttgttg gtacatcttc
    13861 tgctttatcg aagaatcgtt tgtttgcatt gattgttgat tctgttaagt caactactga
    13921 agtcgttccg tcaaatgaag caccaggttc attgaaaccg atatgtccat tttgcccgat
    13981 tcctaagatt tggatatcga ttggatgtgc atcgatgatt tgatcatact ttttgcatgc
    14041 tgcttccaga tcttctgctt ttccatctgg tacaaacgtt tctttgaatg gttttttatc
    14101 aaataattgg acatccatga aatgacggta gctttgttca tctgaaccgc ctagtccaac
    14161 gtactcatct aagtttacag aagtcatttc actgaaatcc aaatcgcttg aagtcatttc
    14221 tttatataaa gtaactggtg tgcttcctgt tgccaatcct aatacttttg cacctttgtt
    14281 cataccttct ttgatgattt caaatgcttt ttttccgcct tcttcagcgt tttttacacg
    14341 aatgatttcc atagtgattg ctcctttata ttttggtata gcccaattat agcattcttt
    14401 ttttcttttt acaataatta gtatagacca aattgcattt ttttcatttt cttgacaata
    14461 aataggaatc cgtgttaggt ttcataagat ggttcaatca aacaatccat gttgcatgaa
    14521 aaccaagcaa gtgattcact gtggacgctt tatgtctgca gtttttttat gaaaaaggag
    14581 ttattatgag tatactgaca ttaaacaaca ttacccagca atttggcgac aaaatcctct
    14641 atgaaggtgt cgatctgcaa ttaaatgctg gcgaacatct tgggctgatt ggacaaaacg
    14701 gagccggtaa aagtacattg attaaaatca tcactggtga gatcttacca gatgacggac
    14761 agatccattg gcaaaaaaat attcacaaag gttacctcga tcaatatgtg gaagtcgatg
    14821 aaacattaac aatcgaagaa tttctaaaaa ctgcgtacgc aaaagaatac gaaaaggaag
    14881 aaaaaattgc ttttctttac caaacatata gcgagacctt cgatgattcc ttgctggaaa
    14941 aagctgggaa actccaaacg gaattagacc aaggagtgtt ttatcaaatc gacaccttag
    15001 ttgctgagat gagcagcggg ttggggatcg atgtcttagg tttagattca ctgttgaaaa
    15061 atctaagtgg tggacaacgt tcaaaggtga ttttagcaaa actactttta gaaaacccag
    15121 atgttctgat cttagatgaa ccaaccaatc acttagatga tcagcatatc caatggctga
    15181 cgcagtttct tcaggatttt gacggtgcgt atatcgtgat ttcccatgac caagaatttc
    15241 ttgatcagat caccacccat atcgcagaca tcgagtttgg aaagattact aagtatactg
    15301 gccatctaaa acaagcattg aaacaaaagg aacaaaatcg cgaaagctat ttgcgccaat
    15361 atcatgctca acaaaaacaa atcgaaaaga ccgaagctta tattcgaaaa tacaaagccg
    15421 gttcccgatc aacgatggca aaaagccgac aaaaacaatt agataaaatc gaacggctga
    15481 cgcctccagc ttcagcaagt aaagctgtct ttgattttcc attctcgcct attgtcacga
    15541 cattagcagt ggaaacaact gatttagtca tcggatatga tcgaccgcta cttgcaccca
    15601 tcaatttgac catccgcttt ggtgaaaata ttgccattcg aggcttcaac gggattggaa
    15661 aatctacttt attaaaaaca ctgatcggtg agattgaaaa aataagtggc gatttccact
    15721 tcccagataa cacaaaaatc aattactttt cacaagatct gtactgggaa aatccattag
    15781 aaacaccact acaatattta agcagtcaat ttccaaaagc gactatcaaa gaattacgtc
    15841 gccaactagc taaagcaggg ttagttaatc aattagcaag tgaacctctc actacattaa
    15901 gtggtgggga acaaactaag gtgaaacttg cacagttgac aatgaaccaa ggtaatttac
    15961 tgattctcga tgaaccgacg aaccatatcg atcaagaaac gaaagaaagc ttacaagacg
    16021 ggatacagaa atttccagga acagtaatca tcgtctctca tgaacaagaa ttttatcaag
    16081 atcttgtgca acgagtcatt gagattgaac caaaataaac gtttgattga atagttgaaa
    16141 aaaaggagct gtgacaaaag ttttgtcaca actccttctt ctgaataaat ggtggccaat
    16201 tagctgacct tcggtattag ctcaaaaaac ggcaaaatat gaggagctat ttttatttgg
    16261 ctattcttat tacttgatgc agaacagctt ttcacaacct cttttcgtct attttttatt
    16321 ttcgagattt ctctttcctt taaaaacaac tgtttgaaag aagaacgcac atccttgaaa
    16381 aacaagcccg ctaaccgtta cacctaacca gccatactgc tgccacattg tcgtccctag
    16441 caatgaacca gccgcacctc caataaagta cataaacata aatacagtat tatttcggtt
    16501 gcttgctgct tcacctagcc cttgtactcg tgtttgattg gcgacttgac caaattgatt
    16561 cccgacgtct aataaaataa tagctaaaag caaaatggcc aaatggcttc cgccaaccaa
    16621 taaagcaata aagcttattg tttgcataac aagtcccata tacacgattt ttctttcaga
    16681 ataagaatct gccagtcgtc ccacgatagg tgcagcaact gctccagaca aaccaaaaat
    16741 cgctaatatc cctgcttcga atgttcccca attgtaggcg ggactgctga tataaaaaat
    16801 caacgtagac caaaagatcg aaaatgttcc aaacatgaaa aatccactga ctgccgcttc
    16861 tcttaataga cgttgagaag caactaaatg cggcaaactt ttaagtgaat caaggtatga
    16921 aacagctgta ctactttgct ggacagaacg cggcattttc cagtaaagca gaagtgtaag
    16981 aattccgact gcacataaag caaataaata gatcatccgc caagaaaaaa gactggcaac
    17041 aataccagat accgtccgag aaagcaaaat ccccacgagt aaaccgctaa gcaatgtacc
    17101 catgaccttc cctcttgctt ttggtcccgc tagtaccgca ccgtaaggaa tgatgatttg
    17161 cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat gcaaataacg taaacgaagg
    17221 cgcaaaaaaa gcggccaaca gagagacggc agataacgct gccattcgaa tgatcaattt
    17281 tggacgatcc accacgtccc ctaaaggaac taaaaacagt aaccctaacg catagcctaa
    17341 ttgggtcaac atcgtcaata tcccgactgc acttgtgcta acagataaac tagctgctac
    17401 ttttgttcca atcggctgga tgtaatacat attggcaaca acaacgccac aagtgattga
    17461 taatagtaaa ataagcgaag ttgttaaagg ttttttttca ttactcttca ttgatttccc
    17521 ccatttccta tcttagttta caacttatgg aaaagattgg aaatagaaaa atacaactat
    17581 tcgccacact tattatttgt gcttttgttt tccttgtttt ttcttgcgat ctttataaat
    17641 gaagacaact aaccctacta gcacagtaac acttgcaacc aacgtgactg cagttttgtt
    17701 atatccgtga aacagagctt ctccaccaca cgcatacaac acagacgaag ggatgactcc
    17761 aatcacgatc gacaatatga tttgctgtag aggcaatttc atcgcatctg cagcaaaatt
    17821 gaccatagaa gatggaataa ccggtatcat atagcctaac attacaccta tttttggatg
    17881 tttcatttgt cggattgctt gcacccaatg attcgtttcg gttttcttat ctaaaaattt
    17941 taagcgatgc ataaggaaaa ttgcagtgat gttaccaaga gcattaccca aaatgttgat
    18001 gatcgcacct gtcagtggtc caaagctcaa accagcaatg actccaacta ctgaagtagg
    18061 aatacctgga acagcacaca tcaccgctgt caatacgatc aagagtatcg atgcaaaagt
    18121 cccatgcgaa cgaatatgtg cgagtagctc tgccttactc gctctcggat ttaaaaataa
    18181 ttggatgtct gtctgatatt ctaaatacaa ccgatagccc actaccagta tcagaatgat
    18241 tcccaacgag atcaatatca atcggattat tttctttttc atatttcctc ctgaaaaaaa
    18301 tctatattca ttaaataata gtttaacata aaaacaaaaa agcaccgaaa tccaacatag
    18361 gatctcagtg cttttaaata gtaaaaatta tttaacagtt acagatgcgc caacttcttc
    18421 aagtttagct ttcaattctt cagcttcttc tttagatacg ccttctttaa ctggtgcagg
    18481 agcgccatca actacagctt tagcttcttt caagcctaaa ccagttgctt cacgaactgc
    18541 tttgataact ttaacttttt ggtctccagc tgcagttaat tctacagtga attctgtttg
    18601 ttcttcagca gcagcagcgc caccagcagc agctacagct acaggagcag cagcagatac
    18661 gccaaattct tcttcaatag ctttaactag gtcgttaagt tctaggatag ttgcttcttt
    18721 taactcagca acgatgtttt caatgttcaa tgccattata atttcctccg tttaattgtt
    18781 cttttttatt taatgcttta tgcggcaatt aagctgcatc tccatcttct ttgtctgcca
    18841 ctgctttgac agcgtaagcc acgttgcgga ctggcgcttg tagtacagat agcaacatag
    18901 atagaagtcc ttcgcgattt ggcaattttg ccaatgctgt gatttcttcc aatgaagaga
    18961 ctttaccttc gatgataccg cctttgattt ctaatgcttt tgcttcttta gcaaattcgt
    19021 ccatgatttt cgctggtgcg actacatctt cgttactgaa tgcaacagct gtaggtccag
    19081 tgaatacttc gtccatgcct tcaaggccag cttttttagc tgcacgtgaa agaatagaat
    19141 ttttgataac tttcatttct acgttagcat cacgaagttg tttacgtaaa cgtgttactt
    19201 catcaactgt taatccgcgg tagtcaacga ctactactga tgcagctgct gtaaattttt
    19261 cgcttacttc atcaacgatt gctgcttttt ttgcgataat tgcttcactc atttttgagt
    19321 ttcacctcct gaattttgat ggaagagttt tttgcactga aaaggcaagt cacttttata
    19381 gttactttgc tcaaccagta ttattcatca cctattcact tcgttcatag tgattctact
    19441 cgctttgctc gaaaggaaac tcctttcgct ttgctcaacc agtattgttc atcatctctt
    19501 cacttcgttc atagtgattc acaataaaaa actctatgcc accgcagaca tagagggact
    19561 attgaatata ttcaataaac gtcctcggta ggaaattaag gcttgatgcc acctactgtc
    19621 ttcggtacaa gttaattatt aacctcaact agcttagcat gctaaactag ttgagtcaag
    19681 aattattttt cagttggatc aatcaacatt ctaatattta attagaaaga agcttggtca
    19741 acatggatac caggtccaaa tgttgttgtg atcgttacat ttttcatgta ttgaccttta
    19801 gctgctgatg gtttagcttt tagcaataca tcatggattg ctgcaaagtt ttcaactaat
    19861 ttttcatcgt caaatgaaac ttttccgatt ggtacatgga tatttccagc tttgtccaca
    19921 cggtaagtta ctttaccagc tttcacttcg tttactgctt ttgttacgtc cattgtaact
    19981 gtaccagttt tagggtttgg cattaagcct tttggtccta atactcgtcc tagacgacct
    20041 acagtagcca tcatatcagg tgttgcaaca actacatcaa agtcaaacca tccgttttgg
    20101 attttttgaa ccatgtcgtc gtcacctacg aaatctgcac cagctgcttc agcttctttt
    20161 gctttgtctc ctttagcaaa aactaaaaca gtttgtgttt taccagttcc gtttggtaat
    20221 acaacagcac cacggatttg ttggtctgct tttttagggt ctacgttcaa acggtatgca
    20281 acttcaactg ttgcatcaaa ttttgcaatg tttgtttctt ttgctaaagc tactgcttcg
    20341 gcaacaggat aaactttact tgaatcaact ttttttaatg catcttgcat ttttttgctc
    20401 tttttagcca ttttggttcc tccttgattg tggttataac ggttgtacct cccacgtgtt
    20461 ctatatcttt caatatagag ccaactgaga agctgctgtc ttagggtgta tagattattc
    20521 tacagtgatc cccatgcttc gtgcagttcc ttcaaccatg cgcattgctg cttcaacgtt
    20581 agctgcgttt aggtcttcca tttttaattc agcgatttct cttacttgat cgctagaaac
    20641 tttcgcaact ttatttttgt ttggttcgcc tgaacctttt tcgattttag ctgctttttt
    20701 caatagaaca gcagctggcg gtgtttttgt aacgaatgtg aatgaacggt cttcgtatac
    20761 agaaataaca accggaatga tcaaacctgc ttgatcagct gtacgagcgt tgaattcttt
    20821 tgtgaatccc atgatattga tacccgcttg acctagtgca ggacctactg gtggagctgg
    20881 agttgcttta cctgcaggaa tttgcaattt aacgattttt tctactttct ttgccacgag
    20941 acatacctcc ttaagtccgt gatgtggtta attggagatg catatttctc ctcccacgtt
    21001 ttatctgatc tgaaaaaatc cagacaaata atgtgcaaaa caatgcacac tggtacagta
    21061 tagcaaattt atttgttttt tcaagatttt tttctgctat ttttacattt tgcttgaata
    21121 aacgctaaat ttattctaaa aaaacaacaa tttttcgttc tcatttcttc ttttatgtta
    21181 cacttgcgct agcatggttc aacagatgaa agcaaacaat aacacggttt tttagaccct
    21241 tgtacaaaga ttcctacata acaaaatgag gtggagaaaa tgaaagattt aaggtataga
    21301 agtgtctttg atattattgg acctgtgatg attggtccaa gtagctctca cactgccgga
    21361 gctgctagaa tcgggaaaat catacgtagt atatttggcg aacagcctga ttctgtagat
    21421 atttatctat atgaatcatt tgcaaaaacc tatcgggggc acggaacaga tattgcatta
    21481 gttggcggcc tgttagggat ggaacccgat gatgaacgtt tagctgattc tttgaaattg
    21541 gcatatgaag ccggtatgga agtcttgttt gttcctaaga gtgaaaaagc cgaacatcca
    21601 aactctgtaa agatgcttct aaaaaaagga acacgaaagc tttcagtcac tggcatttcg
    21661 atcggcggtg gcaatatcca gatttctgaa ttgaatggat tcaaaatttc tttaagcatg
    21721 ggtactccta ccttgatcgt tgtccaccaa gatgttccag gtatgattgc taaagtgact
    21781 agtatacttt ctgagaccaa tacgaatatc ggcactatga cagtgacacg ggaatcaaaa
    21841 ggtgaaaatg ccattatgat aattgaagta gatgatcctc aagtagaaga aacggtaaaa
    21901 agactcaaac agctgccaaa tatcgacagc gtaaattatt tcgattaagg agctaaaaaa
    21961 atgtttttat cgattgaaga attagtagat caagcaaaag cttatccaag tgtagcagaa
    22021 ttgatgattg cagttgaaat ggaaatgagt ggccgttccc gtagtcacat catcgagatc
    22081 atggaaaaaa acctctcagt aatggaacaa tcaatcaacg agggaaaaga aggtgttacc
    22141 tccgtcacag gaattaccgg tggtgatgca aaaaaattag atgattattt aaatgaggca
    22201 gactttttaa gtggagaaac aatcttaaaa gctgtacgca atgctgtagc tgttaatgaa
    22261 gtcaatgcaa aaatggggtt gatttgtgct acaccgactg ccggaagcgc gggtgttgtt
    22321 cctggtgtgc tgatggctgt ccgagatcgg cttgatttga cacatgaaca gcaattggaa
    22381 ttcttattta ccgctggcgc tttcgggtta gtcatcgcaa acaacgcttc tatcagtggt
    22441 gcagaagggg gatgtcaagc agaagttggt tctgcaagtg caatggcagc agcagctttg
    22501 gtttgtgcaa aaaatgggac tcctcatcaa gcagcacaag cagtagccat tacattgaaa
    22561 aatatgatgg gactgatttg cgatcccgtt gcaggcttag ttgaagtccc gtgtgtcaaa
    22621 cgtaatgctt taggctcttc tcaagctttt atctctgctg atatggcatt agcgggtatc
    22681 gaaagtgtca ttccgccgga tgaagtcgtt gctgcaatgt accaagtcgg acgtcaaatg
    22741 ccgtcgattt tcaaagaaac agcagaaggt ggcttagctg ttacgccaac tgctaaacga
    22801 cttgcggaag agattttaaa taagagggcg tgataaagct ccttaatttc gagtattaag
    22861 agattagtta tcttcataac aaaaaacgaa gagaggacta attgcccttc tcttcgtttt
    22921 ttattaatcc atcaatttcg tattttttac ttcatcgatc gtttttgttc ctgcaagttg
    22981 catcgtgatc gacaattctt tgttcaggtg ttcaaaaaca gatgttactc cttctgcccc
    23041 acctagattc aacccataaa tcactggtcg gccaatcgca actaagtcag caccacttgc
    23101 cagagctttg aagacatgct ctcctcgacg gacaccagag tcgaaaatca ctggtacacg
    23161 tttgttgact acttcggcaa tttttggcaa tacttcaaag gacgcaggac ctccatctag
    23221 ttgacgtcca ccatgattgg atacccagat accatctgcc cctgcagaga tagcgattac
    23281 tgcatcttct ggcgattgta ttccttttac gataactgga agatgggtga tttctttgat
    23341 tgttttgatg tcatctggtg tcaagccttg ttttgcagct gcataaattt ctgcgattcc
    23401 ttttccttcg ccattaccac ttgcactttg ttcgctatac gcagctaagt taggcattgg
    23461 taatggaaat tggaattgat tgatgacgtc ttcttcacgg taaccaccga gcgttgaatc
    23521 tgccgttaaa atgatagctt tagcccctgc ttctactgct ttgttcaaaa tgaactcatt
    23581 gaatctgtcg tctttactca tatataattg gaaaaactgc ggtgcgcctg gcgctgcatc
    23641 ggctgcgtct ttgattgtcg tatttgcata agtgctgatg gaaaagattg atccagcagc
    23701 agctactcct ttagctgtat cagcttcccc tttttcatgt gccaatcctt gggccgctga
    23761 tggcgcttgg ataatcggtg tatctaattc aattccgaaa acagatgtat gcaaatcggc
    23821 tgagtcgatt ccgcgtaata tccgtggcat gattttcttt gtattaaaag aagtcgtatt
    23881 ttctttcatc gtccactcat cttcagaacc accgcgaata tagccaaaag ctcctgcttc
    23941 catgcgctct tttacacgac cttctagtta ggcaatgttt acaatatcga ttggatgttc
    24001 ttcattactt gtatgataag tcatagtaga tccttctttc atcttcttga ttacatataa
    24061 atcatactaa acttacaaaa agtaagcaaa catttactct cagatacttt tatcgctatt
    24121 ctttccaaat aaatcgactt acaagctgat ctacttcttg gttttcatga agcatactat
    24181 gagaggcatt ttcacctgtc actacttttt cctctatttc atttccgcgt tgcttcaaca
    24241 acgcatagac cgctaatgca ctgtttaacg gtactgttcc atcactcaag tctgtttcat
    24301 ctagttgacc agcaatcaag aaaaatcgag tggttatagg aatattaccg atcatttgct
    24361 ggtaatcttg atagcggtta gaaacgacag caggcccctt tgctaactca tctgtcagag
    24421 actgcgcact atcatcgaca aaatcattaa aaggcgcgcc aattgctgtt aatgtctgaa
    24481 cttgtggcaa tgacgtatcc tgtccatacg ttcctaaata gcgtagtgca ctaactcctc
    24541 ccatggaatg ccccactatg ttgactttat ctatgccgta gttttctttc agatacaaaa
    24601 gacaagtttt gatccattct gcttgattcc actcgttatt tttattatct tcaaacaata
    24661 cttggaccat cggattgtct gcttttccag acaaatctcc tgttgcttgt atttgtccat
    24721 ctgttcctac tgttaaaacc aattctttta ctgcttgctg atttttttcc aaacgccgca
    24781 acatcccttt aaaggaattt tctgtcccgc tgtagccgtg gaaaaaaagt gttggaatag
    24841 tggttttttc tatcacttta ctggattctg ccgttttttc tttatcagaa acttcagtag
    24901 tatttatctt cgtcgtctgg caaccactta acgcaaacaa actgataagg actaaagctg
    24961 ctgcagttgc acgtttgcat aaatttttga ttggttctgt catacacctc ttcctttcca
    25021 tatagaaaaa agaaagactt gaaaggccaa tcatatgatc gacatttcaa gtctctttat
    25081 taatcgattt tatctacttg atcgaaatca agttctgtac tggtttcacg tccaaacata
    25141 tcgatgttga ctttcaattt ttctttttct ccgtcaactt ctgttacttg tccttctaat
    25201 ccagcaaatg cgccttcgat gattttcacg acttcgccaa cttctacttc tagatcattt
    25261 tgtcgcaggc tcataccgat agaacgcaag atatgattga cttcttctgg aagtaatggt
    25321 gctggtttgc ttccggctcc gtgtgaacca acgaatcctg tcacaccagg tgtgttgcgg
    25381 acaacatacc atgaagcgtc tgtcatgatc atttcaacaa gtacgtatcc tgggaatgtt
    25441 ttatgaacga tttctttttc tttcccattt ttgacttcag tttctttttc ttcaggaaca
    25501 acgacacgga aaataaagtc ggccattttc atactttgcg cacgagattc gatatttgct
    25561 tttactttgt tttcatatcc tgaatatgta tgcaagacat accagttttt ttcaaaagat
    25621 tccattgaag gctcctcatt tcattgtaaa ataaaaaaac ctcagtacac ccgaagtttt
    25681 tctttcgctt cattatagca ctaaaagtac gttgtggcta gcgagttatt ttaaaatcca
    25741 gccaaataag ctttggatgg ctgtatccat tacgtaaaac atcaatccga aaataataga
    25801 agtttcgatg actactaacg tatctttacg gagttgtttt ctgcttggcc aagttacttg
    25861 tttgatttca tcttttacgc tttttagaaa tttcatgaga caacctccct tgctactgtt
    25921 tatttcgttt ctttatgtaa tgtatattga ttacaatatt tacaaaactt attgatttcc
    25981 aaacgttcgc cgctttttcc ttcactgact gattttgtgt agttgcgaga cccacaaacg
    26041 gaacaagcga gtgctgcttt tttctttgcc atttacttct gcccccttgc tgagaatcag
    26101 acttatactt cataacagta tcacgtaact ttttacttgt caatttgtga aataaactat
    26161 ctattgtttt ggataaaatt atgatatgat taatttgaaa ttaagctaaa atgagacaat
    26221 tggaggtata gcgatgctat taaaaacact tgtatataag aaaaaagatc tgacaaccgt
    26281 tacagagact agtacattag aagaagcatt aaaaatttta gaaacttccg gataccgttg
    26341 tgtgccgatc ttagattctt caggtaatat tttccgtgga aacatttaca aaatgcatat
    26401 ctatcgtcat aaagcaaatg gcggtgatat gacgcttcct gtcactcatc ttttgaaaaa
    26461 cgctacaaag ttcatttacc tggatacttc tttcttcaaa gtattcttta cgatcaaaga
    26521 gcttccttat attgctgtcc ttgacagtga aaatcatttt tatggtattt tgacacatag
    26581 tacgttgctt aatatgctct ctcaatcttg gagcgtagaa caaggaagct atgtactgac
    26641 aattgcttcc acaggaaagc aaggagattt ggctgctatt gcaaaaatca tttcaaaaca
    26701 cagcagcatt gccagctgta ttactcttga tatcgaaaaa gacgaataca ttcgacgtac
    26761 attgatcacg ctaccggcta acactgaagc gaaagtatgt gaaacgatcg tcaaccactt
    26821 agagaaaaag aattataaag tggtagaaat tgaaaatttg caaaaccaag aagcataatc
    26881 aaaaagcgaa aaaagaaaac agctaatctg ctttcttttt tcgcttttta taattagaca
    26941 cgcttttcaa aatcaagcgc gcgatctaaa taatacatca atggtgctga aacagccatg
    27001 acgataaact cacttaatgc aagcgtgcca taggttgtcc aaaatgcttg gccacctgtt
    27061 ggaactaaca tataagcaat cagtcccatg ctgatggtaa agaagatcgt gttcaatact
    27121 aaacgcatct ttacattttt cactttgttt tgcaataaag ctgtcaggcc taaagaaagc
    27181 aatgtttggc cgccaccaaa aaccacatcc atgataccaa aaccaaagaa ggcattatag
    27241 acgatgaccc cgcctaaaac tccccaaagc aatttacggt taaagacaac taaatgattc
    27301 aaactttctg aaatacgaaa ctggatcggc cctgaggata caggcgcaac taaaatagtt
    27361 aacgctagat aaagagccat gatgatgcca tttacaacga ttacggataa ccgttgtctg
    27421 cttttcgttt tttgcatttt ctctcctctt ttctcctagt ttttttaacg cgggatggtt
    27481 gatgaaccgc ggttatgtga aacaaacaat aattataaca gacttttcat ttatttcaat
    27541 ccgtcttatc tgcttttaaa aaagcaaaag cttaaacaat gtaaacgttt gctaaaaatc
    27601 gtgggatatg gtataaagta agtgtaaggg gtcataagaa ttcccagtaa agcagacgaa
    27661 gtaggatctg caagaacaga attgttgtga aacttaaata aataaaaaag gatgtgggaa
    27721 tttgagcaac ctcttactag agaacggtct agcaagcagc tgaagatcta agtggacatg
    27781 ccgaaccttg gaacttctcc ttacgtaagt aatggatcat gcgcttgcta ggctt
//
LOCUS       DAYYGO010000021        27611 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000021 DAYYGO010000000
VERSION     DAYYGO010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.021"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(60..587)
                     /locus_tag="ACHA5T_000819"
     CDS             complement(60..587)
                     /locus_tag="ACHA5T_000819"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8886416.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(654..2156)
                     /gene="eat(A)"
                     /locus_tag="ACHA5T_000820"
     CDS             complement(654..2156)
                     /gene="eat(A)"
                     /locus_tag="ACHA5T_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFM8886417.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(2414..3535)
                     /locus_tag="ACHA5T_000821"
     CDS             complement(2414..3535)
                     /locus_tag="ACHA5T_000821"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFM8886418.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3661..4602
                     /locus_tag="ACHA5T_000822"
     CDS             3661..4602
                     /locus_tag="ACHA5T_000822"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFM8886419.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(4752..5648)
                     /locus_tag="ACHA5T_000823"
     CDS             complement(4752..5648)
                     /locus_tag="ACHA5T_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFM8886420.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            5917..7257
                     /locus_tag="ACHA5T_000824"
     CDS             5917..7257
                     /locus_tag="ACHA5T_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8886421.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            7475..7918
                     /locus_tag="ACHA5T_000825"
     CDS             7475..7918
                     /locus_tag="ACHA5T_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFM8886422.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(8422..9840)
                     /gene="nhaC"
                     /locus_tag="ACHA5T_000826"
     CDS             complement(8422..9840)
                     /gene="nhaC"
                     /locus_tag="ACHA5T_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFM8886423.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(10243..11466)
                     /locus_tag="ACHA5T_000827"
     CDS             complement(10243..11466)
                     /locus_tag="ACHA5T_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM8886424.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(11497..12336)
                     /locus_tag="ACHA5T_000828"
     CDS             complement(11497..12336)
                     /locus_tag="ACHA5T_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM8886425.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(12353..13033)
                     /locus_tag="ACHA5T_000829"
     CDS             complement(12353..13033)
                     /locus_tag="ACHA5T_000829"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFM8886426.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(13035..14108)
                     /locus_tag="ACHA5T_000830"
     CDS             complement(13035..14108)
                     /locus_tag="ACHA5T_000830"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFM8886427.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(14164..14402)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(14445..14678)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(14755..15426)
                     /locus_tag="ACHA5T_000831"
     CDS             complement(14755..15426)
                     /locus_tag="ACHA5T_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8886428.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(15513..18161)
                     /gene="mgtA"
                     /locus_tag="ACHA5T_000832"
     CDS             complement(15513..18161)
                     /gene="mgtA"
                     /locus_tag="ACHA5T_000832"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFM8886429.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(18503..18677)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            18850..19287
                     /gene="ndk"
                     /locus_tag="ACHA5T_000833"
     CDS             18850..19287
                     /gene="ndk"
                     /locus_tag="ACHA5T_000833"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFM8886430.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(19309..20175)
                     /locus_tag="ACHA5T_000834"
     CDS             complement(19309..20175)
                     /locus_tag="ACHA5T_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFM8886431.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(20335..22428)
                     /locus_tag="ACHA5T_000835"
     CDS             complement(20335..22428)
                     /locus_tag="ACHA5T_000835"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8886432.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(23177..23800)
                     /locus_tag="ACHA5T_000836"
     CDS             complement(23177..23800)
                     /locus_tag="ACHA5T_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886433.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(23959..25356)
                     /locus_tag="ACHA5T_000837"
     CDS             complement(23959..25356)
                     /locus_tag="ACHA5T_000837"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8886434.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(25388..26359)
                     /locus_tag="ACHA5T_000838"
     CDS             complement(25388..26359)
                     /locus_tag="ACHA5T_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8886435.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            26733..27158
                     /locus_tag="ACHA5T_000839"
     CDS             26733..27158
                     /locus_tag="ACHA5T_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886436.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
BASE COUNT         7942 a         5723 c         4806 g         9140 t
ORIGIN      
        1 gcactgctaa gcaaaagcag tgccttgaaa tattttattt aaataacaca aaaacataat
       61 tactcataag aaaccaattc tgtattatct accatgaatg taaagaaccc ctcattaggc
      121 atcggatgaa aataagcatt gatcactctt atcaaggcat tatggcaaac taacagatag
      181 acattttctt cgtcttcgat gcattctttc aataaaggaa cgatccgtgc atacacatct
      241 aagactgact ccccatttgg gaatcgtaca gcgaacgcta aacgtgcttt ttgaaagttc
      301 tcatctttgc ttggcagtcc atcataatcc ccaaaatcca tttctaccaa tcgttcatca
      361 acggtcagcg gcaagctgag acgatcagca actgcttgtg ctgtatctct tgctcgttga
      421 agaggggaat ggatgatttt cgtgattgga acaggcaaat ctactacctt ttctgctagt
      481 ttctgtgcct gcatctgtcc tttttcagtc agcggcacat ctgcccgtcc gcaaactaat
      541 ccttgtgcgt tccaagttgt ttctccgtgt ctcgttacat aaagcatgtg attctctcct
      601 tcgttggcaa agttcccctt aaccatacct tgttgttctt gagattacaa gggttagatt
      661 ctctcaagag aaataatctc agtagatact ttatccagga aggtttgatc atgttccact
      721 agtaacatgg caggtttcac gttcaagatc agttgttcta attgttcttg attgaagaca
      781 tccaaataat tcaatggttc atcccatgta tatagttcag ctggctgtga taaagattta
      841 gccaattcca cttttttccg ttggcccata ctcatctgct cgatcttgtt atgaaaaaca
      901 tctctttcca tcccaagctt tcggaggttg ttcaaaaatg cttggtagtc tacttggttt
      961 ttctctgcaa attccgccaa cgttcctcga ttgtcttcat aattttggct tgcataacta
     1021 atgctcagat gttttggctg gtatttttct cctataacct tgccgttgaa tgcccccaga
     1081 agataatgga taatggatga ctttcctgca ccgttaggac ctgaaatcgc cacccgctga
     1141 tgaggctcga ttgtaaaatg aattggctcg aataacagat tttcataccc taattgaaga
     1201 tcttctacgc ttaaaagtcg cttatggtga gacgcttggc tattcatcgt caaagaatcg
     1261 atatattcga tatcttttaa tagtttttct ttctcgctga tctgggtctc catccgattc
     1321 acgattgctt tggatcgttt catcgtccgt gcagcatcag cacccactgc tcctttattc
     1381 actcgtctag attcagtatc gatgaatccg acttgtttct ttgttttatc tccttctcgg
     1441 gaacgagacc attcggcttt ttcagctgct gttttcttta gcctactgac ttcttttttc
     1501 aatttttcat tttgagccat ttcaaattca tcacgaagtt ttttctgttc ttcatagata
     1561 gagaaattcc cttgataaag ttccagttga cttttttcga ttgctaaaac atggtccact
     1621 acttcatcga taaatccccg gtcatggctg accacgatga agccttgttt tttctttttc
     1681 aaataagccg ctacttgttt tctaccagag atatccaaat gattcgttgg ttcatcgatt
     1741 aacgggaaat gagtgtcatc cacaaataaa agtgccagta ggacttttgt cttctctccc
     1801 cccgatagtg tgctgaattc tctccataag atttcaggat ctgtttgcat caattggagc
     1861 tctctttcga tttcccatat ctcaaaatcc gtaatatcat ttaacacgta ataggttaaa
     1921 cgttctttat cttttgtctg ttgcgggaaa taggcaaatt cttgctgatg gattacttgc
     1981 ccttgataag gtagtttgtt ttgtagaata ttcagtaaag ttgtctttcc tcgaccgttt
     2041 cgtccgataa gtcctagttt ccattgtgtg tcaaaattta gatttgcttg ttcaaataat
     2101 aatgtgcctt ggctgtcgta gccgaatgtc agatttttta tttcgatttt agacattttc
     2161 catcacctcg taaaaaaaga ccagtcctat tttcagaact ggctaaagag gatgcaaggg
     2221 ttggatacac acaaaaagac ccatacatcg aaaaaaccaa gccaatcccg aaaatctgca
     2281 cactgcttcc ttgtctccac aaggtacagt ctgaatgcag ctttcatcaa tcgacttaat
     2341 ttttcaatgc acggatccct cactttgtcc tatatttgaa attacgatag cataaagaaa
     2401 atgattttgt caactacttc gggaggatgg tgaaaaatct gctaagtgtc tgtcctaaga
     2461 tttttgtttt ttctaatgtt tgcctgtgtt ccattgcttc ttctaaacct attggaccca
     2521 aatgaatact taagacaatt gggagaaaat cagaaagtcc ttctagctct tggtctctac
     2581 ttccgcaaat agcgattatt ggttttcgat atttttttgc aagcactgct acaccgtatg
     2641 gaacttttcc gtggatcgtt tgctgatcga tccgcccttc tcctgtaatg atgacatctg
     2701 cttgttcgat ctttttttcc aatccagtga tttggctgac taaatcgaaa ccagaaacca
     2761 tcttcgctcc caacaaaaaa agggctccac caataccgcc tgctgctcca cttcctggaa
     2821 tatcatctaa atccacctcc cagaaatctt ttatttgctg agcaatcttt cctgcttgtt
     2881 tatctaagaa agctagcgtc gtatctattc ctcctttttg tcttccaaat tgccaagcag
     2941 ctccttgttg acctgtatat ggattggtaa catctgcagc gataatcaat tttactttag
     3001 aaacaagaac gtttcttcga tcaaaatgaa gttttttagt agataataaa ggatttcctt
     3061 ctgtgtaccc ttctaaagaa gcacccaagg attggagtaa tcctagacca ccatcgctac
     3121 acccgcttcc tcctaaagtg acaataattt gttctacttc ttgaagcagt gcatctttaa
     3181 taagttctcc taaggcataa ctggaagcaa gtcggatagt ttgatccgtt ggtttatcaa
     3241 gtagatctaa ccccaatgct tgtgcagatt cgatgactgc ggtgtttttt ccttgccaag
     3301 ttgtcaataa atagctgatt tttcgctgac ggaataagag atccatccca tcaatggtac
     3361 gccatgttcc cctgagcgcg taatagattg cttctgcagt attttctccg ccatctgcaa
     3421 tcggcacatt gatttttctt cctttacgat cttcccagcc atttagaact gcctgattta
     3481 gttcaaatga agaagcactt cctttaaatg aatcaattgc cgccaaaata tccatctgta
     3541 tccctcactt tcttcttaag taagacactt tttttcattt ttttcaaaca aaacaatgct
     3601 attttcttcc atttttctat atactaaaag ggaactcatt gaataaagga gagaaaaaac
     3661 atgaaaatcg caatagctgg ggccggagca atgggcagcc gaatcggatt gatgctacat
     3721 caaagcggaa atgaggtcct cttgatcgat cgttggcctg ctcacattga agccattcgt
     3781 acaaatgggt tgattgctga ctttaacggt aaagaagtgg tcgcaaaact gccaatctat
     3841 tcaccagaag aaatcatcga aagcaacgaa cacgtggact tgatcgtagc attaacaaaa
     3901 gccaatcaat tagatgatat gttttgttcg atccaatcca ttattactga caatacttat
     3961 gttttgtgtt tactaaatgg attaggtcat gaagatgtac tggaaaaata tgtgccaaag
     4021 aaaaatatct tatttgggat cacgatgtgg acagctggtc tagctggtcc cggaaaagtc
     4081 acattgttag gtgatggaga aatcgaacta gagaacttgg aaccagaagg cgaagcattt
     4141 acaaaaaaag ttgtagaagt ctttcaagaa gcaaatttaa atccgatcta tagccataac
     4201 gttcgttatt ccatttggcg aaaagcttgt gtaaatggaa cattgaatgg tttatgcacg
     4261 attttggatt gtaatattgc tgaactaggc gcgcaaaaag cggcagagag catggttcga
     4321 acaatcgtat ctgaatttgc ttctatcgca gcaaaagaag gaatcatact agatcaagaa
     4381 gaagtgtacc aacatattgc ttctacttat gatcctgaca acatcgggtt gcattaccca
     4441 tctatgtacc aagacctgat caaaaaccat cgtttgactg aaatcgacta catcaatggt
     4501 gccatttggc gaaaaggaca aaaatatgat attgctacac catattgtgc ctttttgacc
     4561 caattagtcc atgcaaaaga aggaatcctt ggcgcagaat aaaaggctct ctgatgagaa
     4621 tcagctgaat cagcagctgg ttctcttttt ttaagcccaa taaaagatca aaaagaaaac
     4681 acaactctca tgatgccata agaattgtgc ttgtttgttt cagtacataa tctcttcaaa
     4741 atgtaggttt tttatttgtt ttcaacagag gagaggtcat attcgtattg ctcataaata
     4801 gtggaatcgt tagtgataaa tcgttttgcc caattttctg catcataaaa ttccaatagc
     4861 tctgatagga ttggcttaaa tggtgtatgc agaaattccg gtaattcatc taaatcacgt
     4921 tctcgaagca aaaaaatatt ttcaggacga taaaaatgat aatgagcaat cgcttgatta
     4981 tccgtcacta gtttcttttg aaaatacatg gattcaaatg tccgcaaagt cactccttgc
     5041 tgaccctccc gcaacagttc cagaatcccg cgcgattccc ctgttttttt caggtactct
     5101 tcataagtaa gatatccagg ttgttcattc cctcgatgtg gaagaatgaa atagttggtc
     5161 gtaacatctc tttttataaa ttcctgctta tagctttctg ctcgttcttt tctcccatta
     5221 ttcgttgcac caaaaaatag atccgtcttt ggttcgctag aagaaaaatt catatgtttc
     5281 gaataaaatg aactattatg tttcagccca agttcttttg cttccaaaaa atcaaaatga
     5341 taaatctcat cgattgccgg ttcatcttgt gcctgcttca gcatcgcctc gtgttctgct
     5401 ttgaagtgat tccaaaaaaa taacacaatt tttgcattgg taaatgggcg cacgcgtcga
     5461 gccatctcca tatcaaatcc ataatctaaa aaaacgatca gctgataatt tgccaactgt
     5521 tgtctagctg atttaatgaa ttccgactgt agtaaatctt ttccggacca aacatctaaa
     5581 gagcggatgg aaggcaaaaa ataaagtgat ttattttttg caactagcag tacgtcattt
     5641 ttgttcatct tttccgcctc actttctctg tcactttttc ctatgatagc ctgtttaaat
     5701 agtaaaaagc cagtaaaacg tttcctatat ttgtatgaaa atttaacatt tgtatgaaaa
     5761 tatgatgaaa taaatcattc ttctattaaa attcgcctaa aagttttctg aatagttgta
     5821 attgtctgaa aattttttat cattagtcat tgttaaatat tgctatttat tcttaatata
     5881 aattttgatt gtgaatttaa aagaaagagg tcatttatgg aagataaaaa attgaaaaaa
     5941 caacttagct ccagacatat caccatgtta gctttaggtg gagcaatcgg tgcaggtcta
     6001 tttaaaggaa gtggcgaagc gattgggatc gccggccctt ctgtattgat tgcttttttg
     6061 attggtgggc tgattttata tatcgtgatg aaaggtctgg gaaaaatcgt tttaagcggt
     6121 ggcgacacgc atcacggatt atctggtttg atacgccctt atctaggtgc tcattctgct
     6181 gattttactg attgggtcta ctggtctatg tggattatca atatcatcgc tgaagctgtt
     6241 gcagctgctt cctttttgca gttgtggttc ccgcacattc cagcctgggt atttgttttt
     6301 atgttggctg ttttaactac gatcatcaat ctttattcgg tacggttatt cgcagaaaca
     6361 gaatattggc tggcttttgc taaaatcagt gtcattatct tactgatcat ttttgctact
     6421 tatcttgtcg gtcagcaaat gttgggcaca ggcgttttcc ctacccttca gcaattgact
     6481 gaccatggtg ggtttactcc tcatggtatg aagggaatca tcagttcttt gcttgtagtt
     6541 atctattctt acggcggttc tgaactgatt gctatcacgg taagcgaagc agatgatcct
     6601 aaacgtgcga ttcctaaagc aatcaaaggt gtcatgggaa gaatcatttc cttttacatc
     6661 attcctttgt ttttgctttt gattcttttt ccttggaata ctttagcagg cacgaatgtc
     6721 agtccttttg tgatggtatt tgaaaaaatg aatatcccat ttgcagcgga tatcgtcaat
     6781 tttgtgattg tccttgcttt gttctcatct atcaattcgg gtgtgtatgc atcttcaaga
     6841 attttgttct tccgtttgaa ggatcgaaaa ggttcctcta gaaaactagc tgttttaaac
     6901 aagcatcaag taccgcaacg tgctgtttta ttttgtacca gtgttcttta tcttggtgta
     6961 gctctttctt attttgtcgg tgacaaatta tttggctatc tggcaggttc cctttcgtat
     7021 acagtattat tgatctggat cattatcagt gcggcatcct ttgtactggc actaagaaaa
     7081 ggaacattat gggataaagg aatcagcttt tttgcattag ctgtcttagg attgattttc
     7141 cttggtatcc tgttcacgaa ctccattggg gtaactgtat tgactggcct gctttacctg
     7201 ttcatttatt tcagttatca aaaaaagaat gatgcgttcg tattgacgaa tgaatgaaat
     7261 cgatagaaaa ttttatttta tttatgtgga atagatggag ctattgaaga aaggagttgt
     7321 gacaaaagtc ttgttgcaac tcctttttcc gaataaacag tctcatgacc tcttggtaaa
     7381 cggtgttcaa aaaccctctg tgcaaaataa ttgattgttc tccatgctca tgacatgata
     7441 gaagaaagga atgaaaaaaa ggagtttgtt ttaaatgaaa aagtattgga agtttttgtt
     7501 gatgatcggt gtttcaacgg tagttatgtt tggtatgatg tacttgaatg tctatgcgct
     7561 cgatcatatc ttcttcagtc gtacacgagt attcatggcg ttgatgatgg gtgcagtcat
     7621 ggcagtcatc atgctgttgt tcatgtggaa aatgtatgaa aacaaaaaga taaatgttat
     7681 gatcttagcc gctagtcttc ttgtttttgc cggttccttg tttatggtac gaagccaaac
     7741 tactgtcaac gacacagctt ggatgaaggc catgattcct catcattcta tcgctatcct
     7801 ggcaagtaaa cgagctgatt taaaagatcc tgaagtaaaa aaattagcgg aagaaatcat
     7861 tcaggcacaa gaagaagaga tcgacaaaat gaaaaaaatg atcaaagaaa tggaatgacc
     7921 attatccgtc caaataaaaa aagaggatgt tccagaagtt atcagcttca agaaataaga
     7981 agaagctact tctggaacgc cgtgaataag gggttgtgaa aaagctgccc tgcatcaagt
     8041 aataagaaca gccaaacaaa aatagcttct cctatttttc gtttggttag gcttattacc
     8101 gcagatgcaa gcttttgaac accgtttatt aagaggttgt aaaatcagcg ttctgacctg
     8161 agtattagag agacaaatgg aaaaatagtt cctcatattt tgccattttt tgagctaata
     8221 ccgagggtca gctgattgaa caccgtttat cgagaggttg tgaaaaagtc gcttagtttc
     8281 gcgtattaag aaaaattttg tgaaatggct ttccacattt ttgtataaat ttggcttaat
     8341 accgagaaac tggcttttga ataccgttta ttcgtaaaag ggattgtgac aaaagtcttg
     8401 tcacaatccc tttttatgtt tttattgttc taattgcgct gtcttgctta ctgcttcttg
     8461 cggtttcaca aatttttttg agattcccca acctgtgaag gcactgacca tcgaaaacac
     8521 aggagaaagc aaactgaaaa atacgaatgg caagtaagtg agtgtaggaa taccaaatgt
     8581 acttgctacg aaacttccgg caattcccca aggcactaag tagttgatta ctgttccacc
     8641 atcttctagc gttctaccta atacagttgg atcaaatcct gctttttcat aagtttcttt
     8701 aaatgcatta ccaggcagaa taacagatag gaattgttca ccaataaaaa tatttactcc
     8761 gatacttgtc atcaatgttg taaaaatcaa atttggtgtg ttcgtcatct ttcttgatac
     8821 tcgttccatc acggttgaga tcaagccaaa ttcgatcaac aatccaccta atgacaaagt
     8881 tagaataatc aatgacacag taggcatcat actttcgatt cctccgcggc taagcagtga
     8941 atcgatttgt tggtttccag tatttgaaac aaaccctgat tcgatgaccc cagccaactt
     9001 cgttaaggaa gtttgtgggt tttgaataaa aatcattcct actgctacag cgatattcaa
     9061 caaaatcgtt aaaattgccg gcaccttttt ccatgcacac agtaacatta aaaataaagg
     9121 aatcagtgac cagattgaaa tagtaaagtt tgcttctaga acatcagtca cttcacgaat
     9181 agatgaaaga cttgcggctt tacttgtgtg tcctaaaatc atgaataaaa agaaagaaac
     9241 aacaaatgcc gggacagttg accacatcat atgcttgatg tgtttgaaca aatcggcatc
     9301 aacaatagct gctgctagat ttgttgattc ggataatgga gacatcttat ctccaaaaac
     9361 ggcacctgaa acaatagctc caacaaccag cgctggattt aagcctagtg ttgtcccaat
     9421 accgaaaaat gcaatcccaa tcgttgacat aacagtaaag gcactcccta cagcgctacc
     9481 tacgattgca cacacaacaa atacagaagg gacaaaccac tggacactga tcaatttgaa
     9541 cccaatgacc attagagttg gaatgatccc tgcttgtatc catacagcaa tcaatgaacc
     9601 gactaaaata aaaataaaga ttgggatgat tcctggtttg atgccattga tgattccttc
     9661 gtgcagccaa tctgtcgggt atctttttat cactgcatac aacatgacca ttccgatcgc
     9721 tgctaagatc gtgatattag gtgagacctt taatccgaca acgccaatcg caatgcctaa
     9781 aacaataacc attaatacaa gtgttgcttc taaaaaactt ggtgttcctt tttttcccat
     9841 gataatctct ccttttttag ttgttcttat gttctaaaaa agttaagata tcggattact
     9901 ccgccgcaat tcatccgcga ttcctccttt ataaaaataa aaagacctgt tttaaactaa
     9961 ggacgaatga ctccgcggta ccaccttaat taaaaacaga tctgtttttc actttcaaac
    10021 ttttattcaa atgtatggac tttccatgta attcgtcaat taggcttgat cggttcacag
    10081 cgaccaccga ctttctgaac aaaacaccta accgctactg cgtgaaaagt tgacttgtac
    10141 gaagtttacc ttccataaga tcatttgtca atgaaaaaga aaatattttt tcatttttct
    10201 gaaaattatt tttataactt tcacacacaa gattattttt tactagaaag tagcgccctc
    10261 ttgttttttt cttgctacta tttcttgatg ttcttgcttg attctttcta atagctttgg
    10321 ctctttcaac agatcaagag ctgtattggc gagtagttct gctgcaatcg caatagaagc
    10381 aaggcctttg tcacttttag cagcagcttt gaattcttct gtgtgtccgg caatatatgt
    10441 ctcagagatt gctaccgtag gctggattgt tggtatgact tggctgacat ttcctacatc
    10501 agaagaaccg agacttgttc tttgttccgt ttctatttct tcatctggaa cacctgcttc
    10561 ttttgcatgc tggaagaata agtcatcaaa agaaggagta acgatgacat catcaactgc
    10621 attttggaac aatccaaact caaaagtcgt gcccgtactt atcgcagcac ctcgtacgat
    10681 attttccact ttttcatata ctttatctaa tgtttgccga tttcctgcac gtaaatagaa
    10741 acgtccagca gcgtaatctg gaatgacgtt ggcagctact cctccgtctg taatgatccc
    10801 atggatattg acatcttttg gcaattgcaa gcgtagagcg ttgattccat taaacacttg
    10861 gatcaatgct tccaaagcat ttactccttt ttcaggtgct gctgctgcat gggaagcgac
    10921 accgtaaaat ttgatgtcga ctggatcatt cgctaaagat tctgtcgttt tcccataacg
    10981 aaaagctgga tggacgcata acgctacatc gacatcctca aaaaaacctt ctcgaacaaa
    11041 gctgccttta gctgatccgt tctcgccccc ttcttcccct ggggtgccat atacacggat
    11101 ttctccacca gtttcatcaa tgaattgctt cactatgctg gcggctaata cggagtaagt
    11161 accaaacaaa ttatgcccgc atgcatgacc aatacctggt aaagcatcga attctgccaa
    11221 aaaggcaatt gtgggaccgg gtttattgga aatataacgt gcatcaaagg ctgttcggtg
    11281 tccagctact tctttttcta cccaaaacct ttctttttct aattgtttaa tcaacgctgc
    11341 tgaggaataa aactcatagt tacttacttc aggatgttcg tggatatcta gagcaagtgc
    11401 ttgatattct ggtaaacgat attggattag atccttcaat tggtttgttg agattgttgt
    11461 tgtcataaaa aattcctcct atgctagtca tttttcttat tttctcccga atgtttccca
    11521 tgcaggcacg cttgatcctt ttgatgtttt ctcgattact ttgactgtgt cttctgtttg
    11581 ataagcgtct actatttttt gatacgtttt attgttttca tcttcttttc ttgcaacgat
    11641 gatattcaca taaggacgtg catggtcatc cactggttct aagaagatgg catcagaagc
    11701 aggattaaat ccagcatcta cagctacacc gctattgatc actgaaatgt ctacatctga
    11761 caaagcacgt gctgtttggg aagcatctaa ttcagaaatg tctagatttt ttctattctc
    11821 ggtaatatca gaaacagtgg gcgcttgtcc ttttgcctta tccactttga tcaatccagc
    11881 agtttgcaat aacagaagag ctctgccgcc atttgtaaca tcattaggga ttgctacttt
    11941 tgctccgtct ttgatatcag ctacatcctt caaagtgtta gaataaattc ccagcggtgc
    12001 attgacagta tttccgatcg atactaaatc agttccgtgt tctttattgt aattgtctaa
    12061 aaagaattga tgttggaaag aattcaaatc aatcgatcca tctgctaaag ccacatttgg
    12121 ttgattgtaa tctgtaaatt tcacgtattc caaattaatg ttctcctttt tcaaacggtc
    12181 tttcacgttg tcccatacat ctgtatcttc tccaataatc cctagtttta cgatagtttc
    12241 ttttttgtca gttgtatctg cttttccttg gctacaccct gccaaccaaa taattgctac
    12301 aaatgtaatg actacaaaac gaataatttt tttcattttt tctcctccac tttcaatgtg
    12361 taattttttt aatcagttga ttactgataa attgacttaa aaaaactaag attagaatca
    12421 ataatgtagc cacaatcgtt acatctgttt ggaatcgatt ataccctctc gatatagcaa
    12481 gattccctaa tccgcctgca cctactgctc cagccatggt agtcagtccg atcacgttga
    12541 tgatagtcaa cgccgagacc cgaatgatac caggcaaacc ttctaacaaa tacacacgta
    12601 aaatgatacc gattggactg gttcccatcg cttctgccgc ttcgatcacc ccaggatcaa
    12661 cttctagcag tgcgttctcg atttgacgag caaaaaacgg aacaactcca aaaatcaatg
    12721 gcactaaagc tgctttcgca ccaatcgtcg ttccaactaa gaaacgagta gtcaatgcta
    12781 aaagagaaag caagataatg aaagggatgg aacgaatgat attgatcagc ttatcaagta
    12841 tttcaaatag cagacggtta gctagtaatc cattagggcg tgtcactact aacgtcacac
    12901 ctaagatgat tccaatcatc ccgccaatga atgctgtcca aaaagacata tatagtgttt
    12961 gcttcgtcgc ttcgacaaat tcatccggaa tcaaataaac attgggcaaa tatgttttaa
    13021 agaaatcagt cattttatct tcctccttcg atcactttga tacgactatc taaatcgttt
    13081 ttttgtaaaa tatttatttt tacaccagat ttgatcaaat aatccaatgc ttttttccgc
    13141 tgtttggttt ctccagctaa agtaacgatc aaactcccta acggcgtttc ttgtaataac
    13201 tctacattgc catataaaat attcgctgtt acttgatatt tgctgtacag atgagcgatt
    13261 agaggttcgt tcgtctgatt tccaatataa cttaattcca ccagccattt attggcgatc
    13321 tgatcggcaa aagcagaatg ggcaatgatc gtttctaatg cttggtcaag atgagtagct
    13381 gttcgaataa aatctttggt caattcttct tcaggattgc tgaagatctg gatgctgcta
    13441 cctttttcaa cgattcttcc atcttccata accgctacct tgttgcagat ttctttgaca
    13501 acttgcatct catgagtgat caggacgatt gttaaaccta actggcgatt caattttttt
    13561 aacaatgcaa ggatctgaag ggtcgttttc ggatcaagtg cactggttgc ttcatcacat
    13621 aataatacct taggttcatt cgccaaagct cgtgcgattg ctactcgttg tttttgtccc
    13681 cccgacagtt gtttaggata agcagatgct ttttcttcca aaccgacaag ttccagtagt
    13741 tcattcactt tttgacgacg ttcctgtttc gattttccag aatactttaa agaaaaatca
    13801 acgttgtcaa aaatagttcg gctttccatt agattgaaat gctgaaagat cataccgatg
    13861 gcctttcttt cttttcgcaa ttctttggga gagagctgac tcagattcgt ctgattgaca
    13921 atgacttctc ctgctgttgg tttctgcagc aaattcatca cacgaactaa cgtacttttc
    13981 cctgcacctg agtaaccgac gattccataa acatcgcctt tttcaatcgt taaatctaca
    14041 gaatctactg cctgtatctc tttttctttt tgtttgaaac atacagaaac atctcgcaat
    14101 tcaatcatta aaattcctcc ttatctaaaa aaagcgtcct caagaaaatg aaaatgtcat
    14161 tttcttgagg acgctttttt gcgtgttacc acctcatgtt caagctgtgt tcacacacaa
    14221 ctcctcttca agtacgatca tactctagca agatatcggt cgcagccgtc atcgccttac
    14281 atctatgctc gggatgaatt gctcgatggc cattttccaa tagtcgttcc ttgcttcttc
    14341 tcagctaccg aagctctctg tcaaaggttc ttctatcgta cttaccattt caatgcaaat
    14401 aatattcaaa aaaaaagtcc ttttactaac ataaactatg tcagtaaaag gacgacgtat
    14461 cgcggtacca ccttttttcg gaagtttcct tccctcaagc ccaacatctc aaaaagagag
    14521 attggcgttc gttttaacgg tcgaacagtt ccgaatttgc ctacttatcg acaaattatt
    14581 ctccaaggcc attttcagct tttcttactt gactcttctc accagccaga gcctctctgc
    14641 aaagtttcca aacttacttt ccttttcaac gaaatcgata ttttgttgtt gggattaatc
    14701 tacatgagga aaactgaaaa gtcaagtacg aaattcaaat tattttttct atatttattt
    14761 ttctgttttt ttatttaaca tttcaataac agttcgaaga tcttttcctt ccgcaagaag
    14821 tttcaatttt tccttttctt cgacggtata atttcggaca aaactggaat caggtatcaa
    14881 aacaacgcct aatcctgctc gtgaagcagc tgttgctcct actaaagaat cttccactgc
    14941 aaaggctgaa ctagcttcta atcctgttcg ttccaaagct gtcagataag gaatcggtga
    15001 aggcttgttc tccgacactt cgtctccgaa tgtaaacgtg tccaagtaat cagctaattt
    15061 aaagtgatcc aagatatctg ttgctctttt cgtaacagtg gtcgtagcaa tgcctagttt
    15121 catatgacgt tcttttaagt ctgccaatat ttcttttgca tatggtttta agtggatggc
    15181 tccgcttgtc agctgttgat aaaaatattc ttcacgcttc gctcggactt cctgtacttt
    15241 ttcagggcta cctgctgctt tcactaaaat atcaaaactt tgtttccagt tttttccgcc
    15301 ccaagcgtca acgatttctt ttgggatatg gatatcaaat tgctgaaatc cccaaagcca
    15361 gccatcacga tacgtttttt ctgtgtcaac taacaaacca tctaaatcaa atattgcacc
    15421 tttcatctat acattaatcc tttccaaaac aatacacata gtttagcacg actggtccaa
    15481 aaagaacaga agcaaagaac cagccttctc gtttataaga gttcttggaa tcgacggata
    15541 tagattttct tcacagctgt cacaagcgcc agataagcga tgactgtagc aaataaccat
    15601 aaccagtaat tgccaggaag tggcatcaag cctaattcag caccaaaact tgtaaaagga
    15661 aggacagaac cgacaataat tcctaatgta gtgataacaa acatggcaaa tgttgcgttg
    15721 ctttggataa atggtacttt cggtgtacgc agtgcatgga gcactaaggt ttgtgtccat
    15781 agtgattcga caaaccatcc tgcatggaat aatgcgatga aaatcactcg ctgtgaacta
    15841 tccaatgtat ggaaacttcc gcccactaca gcaggacaaa taacaaaata catcaacaag
    15901 taagtagtaa tatcaaatat cgaacttgtt ggaccaaagt aaaccatgaa ttccccaata
    15961 ctagatgcat tccatttttt tggctcgtcc aaatattctt tatccatccg atcccatggc
    16021 aaggaaatgc aggaaacatc atagatcaga ttcaaaaaca aaagttgtaa aggcagcatt
    16081 ggtaaaaatg gcaggaacgt acttgcaatc agcacggaaa acatattgcc aaaattagaa
    16141 cttgctgtag ccttgatata tttcatgata ttcccaaaag tttctcgtcc agaaagaagg
    16201 cctctttcca aaatcgtcaa atctttttct aaaaggatca cgtctgcaga ttctttcgca
    16261 atatccacag ctgtatctac agaaataccg atatcagcca ctttcattgc aggagcgtca
    16321 ttgataccat ctcctaaaaa tccgacagta tgaccatttt tacgtaaagc tgtcgtcaaa
    16381 cgtgcttttt gctgaggatt caattttacg aatacttcat ggtcttcagc tacttgtgct
    16441 aattcatgat cactcatttc cgagattttc tcaccggtaa ccaattcatt gatctctaga
    16501 cctacttgac ggcacactga acgtgtaact aattcgttgt ccccagtcag aacttttaca
    16561 gctacaccat gttctttcag agctttcaac gcttgtttcg ttgtttcttt tggtggatca
    16621 aggaaagcaa gataaccaat caaaaccatg tctgactcat ctttgactga aaattctcca
    16681 acgacgcttg gattggtctt ttgtgcgaca ccaatcactc gtaagccatc ttcattaagt
    16741 gactgtactt ttgcaagtac ggcttctttt cgttcttttg ttaatggtgt aacagtaccg
    16801 ccaagatcaa taaatttaga aatgttcagc atttcttcaa tcgcaccttt tgtgatcatt
    16861 tgtgttttac cagctggatc agcaaccaca acactcatac gacgacgttc aaaatcaaaa
    16921 ggaatttcat ccactttttg ataattgatc ttatctgtat ctaaagtttc tgttgcttca
    16981 tcaatgatag ccacatctaa caaatttttc agccctgttt gatagtagct atttagatac
    17041 gcatggcgca aaacacgatc atcttcattt ccttcacaat ccatatgata ttcaagaatg
    17101 attttatctt gtgtcaatgt ccccgtttta tccgtacata acacatcgat tgcaccaaag
    17161 ttctggatgg cattcaaatt tttgatcact gttccttttt tggccatcac acttgctcct
    17221 ttgaccaaat tcgtcgtaac gatcattgga agcatttcag gtgttagacc aactgctaca
    17281 gatagtccaa aaagaaaagc ttctagccaa tccccttttg taagcccatt gatgacgata
    17341 actgctggag ccatcaatga cataaatttg atcagtagca tggatgtttt atggatgcct
    17401 aattcaaagc ttgattgaat tggtttttca gatagcgttt ctgctacatg accaaatagt
    17461 gtttcatttc caacagaaac aatgattcct tccgcacttc cactgatgac attacttccc
    17521 ataaagacaa gattttcgta acttgtttcg ctttgctgat cgtcttcaca tactttcgca
    17581 tgcttttcta ctggatagct ttcccctgtc aatgctgctt gggaaatgaa taaatccttt
    17641 gcggacaata aacgcatatc cgctggtatc atatcccctg ctgaaagccg aacaagatct
    17701 ccacagacga tttcttccat aggaatctct gtgtactttc cattacgttt tacagctgcc
    17761 gttactttca caagtgattt cagtttttca gccgcttgat tggaacgtac ggactgaatc
    17821 aacgtcattg tcccgctgac gataaccata gtgaagataa tggctacacc gaaaagatct
    17881 ttatctccag gagctgctaa tacatattcc gtgatgaatg aaataattcc taatgcgatc
    17941 aacactaatg taaatggcgt gatgtatgct ttcaatattt ctacaatgaa tggtgttttt
    18001 ttgccatatg aaatcgcatt ttcaccgtaa ttttctcttg aacgttcgat ttgttcttct
    18061 gtcaatccat ttgctgatgc atggaatagt tcaaataatt cttgtttcgt ttttccagca
    18121 aaagctgcat agattttttg tgtatctttc tttttttcca tctcaatctc tcctttgtgt
    18181 ccaaaagaca gctgttctcg ctgcttttgg tctttagcta ttcatcggat cgatagagaa
    18241 aaaactgaaa ggggctgaga acagatagat aatagccctg gcctttcctc tagatccttg
    18301 tttttcgaat gactgtcatc catttgttct catctccttt actaagtaat tttaattcat
    18361 gacttccaat catttttcga atagttattt ttccaattag accacctcct ttttattttt
    18421 caccgatggg gatttgcggc aacaaaaaaa cctctcgcca aatactcagc gaaaggtttg
    18481 aaaaagcata ttaaaaaaag cttctcagat ctcatcgttg agttttgaca ctgtacaacg
    18541 taaaaagcag atacctgctt ttagctacct tatgaaaagc cttatctcga caattcctgt
    18601 tctacccatt ggcgtctctc gacgtttttg ggcagtagcc tatgtttgta taggagcctc
    18661 acctaacaaa tcttattcgg ttttccttcg ttaatataac aactaaaaaa gaggatgtca
    18721 aatcaatttt ccatgttttt catacttttt ttaaaataca cacgttatat tcctgtctca
    18781 cgcggtttta tttgtctcat tttccacctc ccgctataat agaaatatag taggaaacga
    18841 ggtgctgtta tggaaagaac acttgttatt atcaagccag acggcgtgag acgccattta
    18901 gtcggaagca tcatccaacg ttttgaagca aagggactgg ccattgctga aatgaagttt
    18961 gaaactatga ctcctgaatt agcaaaagaa cattatcagc acttgactga gcgcagtttt
    19021 tttgatgaac tgatcgatta catgacttcc ggtcctgtcg tttatcttgt gctggtcggc
    19081 gaggaagtga tcgatatcgt ccgtaagatg gttggagcaa caaaagctgc cgatgctgtt
    19141 ccaggtacga tccgaggaga ctacgcgctg ccggggactg aaaacatcat tcacgcttct
    19201 gattcccgtg atgctgcggt aaaagagatc gcccgctttt tcccgccatt cgatataaac
    19261 aaaccgtctg aaaaagcggc gaagtgatac aaattctaaa ctgctggtct agttgctggg
    19321 ttcagcaatg gccagcagtt ttttctctaa ggacctccgt tgattctttc ctaacatata
    19381 ttcgagcact gtgaagaatt cttggtagtc tttcgtcttt tcttccccta aagcagcaga
    19441 aatcaatctt tgataataat ctgccatcaa tgtttcatag atcactgtac ttctttcttt
    19501 atgtgctaag ctccgtttca tatcttcatt cacactacgt gcctttttca accgaccatt
    19561 ttctatataa aacgaagtca ggttcattaa aaaagtaaag agatactgct catgattgtt
    19621 caactgtgct aatcgtcgca ttcttttttt gattttcaaa taattgttct caatgaaccg
    19681 atcactgaat atccaaggaa aatcagctag aatagatagt tcaaattgac catagtctgg
    19741 tatctgcatc aagtattctt caatgattcg gcattcataa cgtaaaaatt caaattctgt
    19801 ctcagatact tctgtttttt cgaccatgtg aagattcaaa accaaataat acagatcttc
    19861 tgttttctta aaatgcctac ggttattgct gatctcttta taaggatcaa agctgtcttc
    19921 ttgtacccga tctagcattc gtcttttttt ctttaacttt attggttcat ccagctggtt
    19981 tcgcatccat tcatattctg caagtgtcat gttcattcgc tctaaaaatg atctcaatgt
    20041 tacaaatgac atttttttca ctgaatttgt ctcaatcccg actagagaag tcttaaaaca
    20101 gattccttcc gctaattggg actgtgtcaa cttcttgttg actcgaagtt tcttgatcaa
    20161 ttcaccatca tacataatag ttctcctttt taatttaaat ttttccacat tcaataaata
    20221 gatacgttaa aaaggaaaaa tacacgaaaa aaataaaaaa atgttttttt cataaaaaat
    20281 accaaccttc aagcattaga atgttgtcta actactgaag gttggtttga ttgtttattg
    20341 attatttttt tgctgttcaa cgtctgttct tgttactgga tataagttgg ttgttgtttt
    20401 attaccattt tttgaaaaga cacttgtcga atgttcgcct ttttgctttt cgattgcttc
    20461 attacgttcc gcttcatttt tatagtcata ttttttcgga tcaatcgtct caaatccgtt
    20521 tggcacataa aaccgcaaga gattttcctg attcagctta tctgaaagac ttaacgcagt
    20581 cttgactttt tcctgatctt tggagatttc ctcatcgatt cctgctgctt tcgtatcgat
    20641 gatttctcct gtctgattat tgtaagcttt tcctcctagg acggtgtatt tgggtgtcac
    20701 aaagttccca tttcgaaaag caactacttg atcatgctga gaagataata agtctgtacc
    20761 aaaatgaaga taatctttat catcgattcc caatagatgt agtaatgtcg gcaaaacatc
    20821 gatttctcca ccgtattcat gattgactgt ccctttagta taaccaggca tatggatcat
    20881 caatggcact cgttgcatct gtgcgttgtc aaattcatcc catgtatctg gatctttacc
    20941 aagagcgctt gctaaatctt tattatctgt attcgagatc ccaaaatgat ccccgtaaat
    21001 cacaaagatt gaattttggt aaataccaga ttttttcaaa tacgtaaaaa attgttccaa
    21061 tgattgatcc agataatgtg ccgttcgaac ataattatct actgtgctat tgccagtatt
    21121 caaagatgga aagtcgaaat tcttgtcatc cgtataataa ggcgtgtggt tggttacgga
    21181 aagaaatttc gtgtaaaacg gctgttgtag atgctcaaga tattgggcag attctctgaa
    21241 taaatcttta tctaatatcc cataacctaa cgtttcgtcg gattcatcaa aataagatcg
    21301 atcaaagaaa ttgtcataac ctaagttttt atatacatga tctctattcc aaaaactagc
    21361 tacattccca tggaaaacag cgcttgtata gccttgcttt tgtccaagga tcgctggtgc
    21421 ggcttgaaag acattgtctg acccaagttc tgtgaataat gagccttgag gtaaaccaaa
    21481 tgtgccagtt tccaacatat tttcagcatc acttgtcttt ccctgtccta cttcatgaaa
    21541 gaaattgtca aagctgatcg ttgcttgatt ctggaaaata ctatttaaaa acggtgtgac
    21601 ttcttgtccg tctaccttca tattaatcaa aaactgctgg aaactttcta aatggagaac
    21661 gatcacattt ttccctttag cgatcccaaa tgtttcggga tttggctctg cataatgttt
    21721 ctttgtataa tctaaaactt catcaatccc attactgctt gcatgcgcgc gaacactgct
    21781 agtcattcct gttttgattc cgtcataaat agtatacgca tcgattccta gatatttgac
    21841 aatatatgaa cggtcaaatg ttcgctgcag caattgtgga cggttggctt cgctcagcgc
    21901 tagatttcct gaaaaaataa gccctgaaag acaggtaaca gcgatagcca tcggtttgcc
    21961 gacagggttg cttttgatag gtattttctt gaccttcaac caaaccagta gcccaatgaa
    22021 cacagcgata tccagccagt agatgacatc atggggcttc atcaaagaaa aggagcttcc
    22081 tgacaatcct tgcgacactt tcgaaaagcc taatacagat ttgactgtaa tgaaatctgt
    22141 gaattcccga taaaaaataa tattcaagta caacaaaagc gtatttaaaa tatcaatgat
    22201 caaaagaata gctaacgctg gctttggctt tttaatatac aaagaaaggc caaaaaacaa
    22261 taaggtagtc gcaattgggt tgatccacaa gataaagtac tggatcgtac cttccacacc
    22321 taatgaaaaa tctgcatagt aggcaatgat cgtcttcagc cagaaaaaga aaacagctgt
    22381 caaaacgatt gctaaacgat tttggcacaa ttttttaagt tgtgtcacgt atatcacttc
    22441 tctctattca cattttttct tattttgctc ctgaaaaatg aaaatttcct aaaaaagttg
    22501 ttatcttaag atttttttat tctttctaaa gattattccg tccacttttc acagcataaa
    22561 accgctgcga tgtaagcgat taaactaatg aagagataaa agtcttgtgg tgaccgcttc
    22621 actccacggt attcaaaagc cagtttctcg gtattcgtac aaaaaaagag gctgtgacaa
    22681 aattcttgtc gcagctcctt atttcttgat gcaggacagc tttttcacaa cttcttcctt
    22741 aaacggtgtt ctcctgccaa ctcctcggcc acaagtcaca atatttataa aacatgagaa
    22801 gctgtttttt gaaattgttc cttgtggctc agagttaaac ggcaggctca agacctcttg
    22861 atacacggtg ttcaatcagc tgaccctcgg tattagatta tgtcaggacg ctaattttac
    22921 aacctcttgg taaacggtgt tcaaaagctt gcatctgcgg taataagccc aaccaaacga
    22981 aaaatataag gagctatttt ttgaaattgc tccttgtggc tcagagttaa acggcaggct
    23041 caagacctct tgatacacgg tgttcaatca gctgaccttc ggtattagct caaaaaacgg
    23101 caaaatatga ggaactattt tgccgtttgt ttctctaata ctcaggtcaa aacgctgatt
    23161 tcacaacctc tgtttttcat tgtccatagg attgatattg tccttgtgat gactcattcg
    23221 taataattga ggcttgattt tcttcacttc ccgttatcgt ttgtccactg ccagaatctg
    23281 aaggacttgt tcctgtcgac tcgttttttt gagaagagac agttgttttc ttgacttgtt
    23341 catttgtctt tttccattcc gctaaacgat cggcgaccac ttttttgtct actttttcag
    23401 aataagcggt gatcactttt ccatttttca aacgggctac tttgtcgcca tcatgagtca
    23461 aaagcaaaac ggtcaaatgc ttgtctttaa attgatattg tctgagagaa atcgcactaa
    23521 gtacgtcatc gatattaata aattgatttt cttcttttcc ttttggcaaa taaatgtcca
    23581 attcgccttt ttcgtaattg gcagttattt tatactccgc ccaccaataa gttgagaatt
    23641 cttttttgaa attatctact gattctttgc ttgctacgat tttttctgtt tctttctgag
    23701 caagatctgc tgtcattttt cctgaaaatg ctgcggataa aataagcagt aagaaaaaac
    23761 cgaataacca aaagagaaga aacggtcttt ttttcctcat gctttcctcc actcctattt
    23821 aattttgact attctattat atacaaaaaa tgaaatagaa agacaaaaaa gttgaaaaag
    23881 atcgtaaata attgaacttc tttttcacaa aatggtaaga atggagaatt tcacgcattt
    23941 gctctcaact attctttctt aagcctcaaa tccgttttta tctgatacgt ctttgaacca
    24001 atgacccgat tttttgattg ttctttttcc atcttgtgct aagtctactg aaataaagcc
    24061 atatcgattc ttatatgcat tggtccacga ccagttatcc atacatgtcc acatatgata
    24121 gcctaaacaa ttggcacctt cttgaatggc ttgatggaca tatttcagat gagattggac
    24181 aaattcgatc cgataatcat cgtggatcat gccatcttct ttgataaatc gtgcttcgtt
    24241 ttctacaccc ataccgtttt cagaaataaa gcagtcgata tttccataat gatctcgtac
    24301 attgatcaaa atatcataga ttccttgttc atagatctcc catccgcgat aaggattcat
    24361 tttacgacca ggcatttcat aattgtcgaa ataatcctca ggcattggac cgtttttagt
    24421 atcgattggt gtttcttttg cttttacccg gcgtggctgg taataattga tacctaaaag
    24481 atctactgta ttttctttga taaccgtcaa atcctctgca tggatcacag gcatgagatc
    24541 aagttctttt actatctcaa tcaattcact tggaaactcg ccttttacag ctggatcaag
    24601 gaatgaacga ttgaagaacg catctgctat ttgcgctgct ttaacatctt cagggtttga
    24661 ttcgtctctt ggatagcttg gtgtcagatt caagatgata ccaataacac caccttgctc
    24721 catttcatgg taagatttga tggctaacgc tgaagccaac gcttcatgat agccaacttg
    24781 gatcgctttt ttcatatctg actcttctgg ataatgccat cgatataaat aacctgcttc
    24841 gactggaacg attggttcat tatgagtaaa ccattttttt actttcgaac caaacaattc
    24901 aaaacaactt ttggcatatt tttcataggc ttcaaccgtt tctcggttca accatccgcc
    24961 tttttcttgc agatacatcg gcatatcgaa atgatacaga ttcacaaagg gttcgatacc
    25021 gttttcaatc aactcatcaa aataacgatg atagaaacga acggcttctt cgttgattgt
    25081 tccagattca tcaggtatca aacggctcca ttggatagat gttcgaaatg aattatgccc
    25141 agtctgtttc atcaactgta catcctcttg atacttcgta taaacttgcg aggttttttc
    25201 aggtcctact tgatcaaaga acttttgcgg ttcttttttg taccaataat caaaaatgtt
    25261 ctctcctttt ccatcgcctg aaatccttcc ttctgtttgt gggccacttg ctgcagaacc
    25321 ccaccaaaaa tttgctggaa attgatattt tttcatcaaa gcacctcttt ttctttttta
    25381 gttcgtttta gttgattcca tgtatgatat tttccatcac aatcactttt tcattttctt
    25441 cctcttcttt tttgattcgt tttaacagct gttctgctgc tcgttccccg attttatctg
    25501 ttggtggcgt gatcgtgatt ttcggaagga tactcagatc gtttttatct aaataatcat
    25561 agtgactgaa cgcaatatcc ttcccgattc tgacaccttc ttgattgaaa cattcgatgg
    25621 caccaatcga catattgtaa ttgctgacaa aaatagcagt tgcccctttt tctaataatg
    25681 cttttgcccc gtaatagccg ctttttctag aatagtctcc tgtaaagacc tctaattgga
    25741 tcgatggatc attttggacc gcttctttgg ctcctgctaa acgttctctt gctacgtagt
    25801 ctgtttgagt ggcagcaata aatccgattt ttttatgtcc ttgttcacca tgatgagcga
    25861 tcacttttgc tacactttct cgatcattca ctaatattgt atcaatacct gctggtcctt
    25921 ctggacaatt caatgcgata actggaatac tcatttttgt gagcatttca aatccagacc
    25981 actcttcttc ggataagaag acaatcagcc catcgatcac atgcttcatc aggtattcaa
    26041 ttttttgttc aattttttct ggctgatcgg caaacccgct taataataag ctatacccat
    26101 tttcttcagc tactctttcg atccctgaga caacttccga cccaaaacgg ctggaaatcc
    26161 cgtttatcaa aagtccaata gagaagcttc gattattctt caacccttta gcaatgatgt
    26221 tttctttata gtccaacgct gaaatcgctt ctgctatttt tttcatgttc ttttccttca
    26281 gttgctgacc attcaagtaa cgggaaactg ttcccactga aacaccagcc atcttagcca
    26341 catcacggat cgatgccata cattctcact cctgccagtt acatttgatt tctctttctt
    26401 gttgttcgat tatgatgacc gcattctttg catagtgttg cttgtcatca atatccatat
    26461 aactaaagtc ttcagtagag caacttcctc tcactattaa ttatacttaa acaagtattt
    26521 gaaccggttc atttttaata tagcattttt ttaaaagatt gcaacacttt tttaaaacgc
    26581 tttctgaaag tggcggattt tttgcctttt taaaatttta ttctaaaatt tactctattt
    26641 gtgggtgttt tgtgcgtttt tctcttccat ccgtttgaag ttttacattc caatggtata
    26701 atttctttat cctatcaaat gggggaagta ttttgaagaa attaatcgta ctatttattt
    26761 tgtctgcggg acttttgaca ttgactggct gtgatttgtt tggcggctct gaccaatcct
    26821 cggacgaaac aaccgattct tctgtcttaa aagcggaaaa agcagatacc accaacagtg
    26881 tatatacgct aaaagtacgt aaagatatgg atttatccgc gggacagttg acatttacag
    26941 ataaaaaaat ggaatggaca cgcacgtatc agacacaagg aactgattct tctgaatcat
    27001 ctgaagaaaa aacagtgctg acagatatca agatcactac caaagatgac gaatatatta
    27061 tttcaggaaa agaaaataaa aaagaaacaa cagtcatatt tacaaaaatc ggtaattacc
    27121 gtttgaagga tgaggatggc aacatctaca gtctatagtg ccgtctgttg gatcttcttt
    27181 tgtttctccc cttcttttct gctatagtaa agaagcaaga tgattgtaaa ggagacgaaa
    27241 tggatgatcg atagtcctga ttattgtaca tgtaataaca acaaaaatat ccatacagaa
    27301 tttaatgacc aaggaagctt gagcatatgc gacaactgtc acaaactagt caaagattca
    27361 tttaaaacat ttgaatctta tcctgttcaa aattatgtaa aaagtagaag aagataaaca
    27421 aatccaagca aaactcacta tggctgattt ttatgaactt caaactcagc ttcttccttt
    27481 gatcaagctt catcagtaaa acaaacagtg gatcatttca gtttacaaat caaagagtaa
    27541 agatttattt atttcaaata tttgaatata aaataaccaa acgatagaaa ggcttattct
    27601 acatcacgta g
//
LOCUS       DAYYGO010000022        27510 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000022 DAYYGO010000000
VERSION     DAYYGO010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.022"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(21..965)
                     /locus_tag="ACHA5T_000840"
     CDS             complement(21..965)
                     /locus_tag="ACHA5T_000840"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFM8886437.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1261..2205)
                     /locus_tag="ACHA5T_000841"
     CDS             complement(1261..2205)
                     /locus_tag="ACHA5T_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFM8886438.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2426..3640
                     /locus_tag="ACHA5T_000842"
     CDS             2426..3640
                     /locus_tag="ACHA5T_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8886439.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3662..5677
                     /locus_tag="ACHA5T_000843"
     CDS             3662..5677
                     /locus_tag="ACHA5T_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFM8886440.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(5760..7133)
                     /locus_tag="ACHA5T_000844"
     CDS             complement(5760..7133)
                     /locus_tag="ACHA5T_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFM8886441.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7159..7443)
                     /locus_tag="ACHA5T_000845"
     CDS             complement(7159..7443)
                     /locus_tag="ACHA5T_000845"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8886442.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7445..9460)
                     /locus_tag="ACHA5T_000846"
     CDS             complement(7445..9460)
                     /locus_tag="ACHA5T_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8886443.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9639..9944)
                     /locus_tag="ACHA5T_000847"
     CDS             complement(9639..9944)
                     /locus_tag="ACHA5T_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFM8886444.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10033..11064)
                     /locus_tag="ACHA5T_000848"
     CDS             complement(10033..11064)
                     /locus_tag="ACHA5T_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8886445.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11271..12146
                     /locus_tag="ACHA5T_000849"
     CDS             11271..12146
                     /locus_tag="ACHA5T_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8886446.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12298..13140)
                     /locus_tag="ACHA5T_000850"
     CDS             complement(12298..13140)
                     /locus_tag="ACHA5T_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8886447.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13232..15109)
                     /locus_tag="ACHA5T_000851"
     CDS             complement(13232..15109)
                     /locus_tag="ACHA5T_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM8886448.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15106..16527)
                     /locus_tag="ACHA5T_000852"
     CDS             complement(15106..16527)
                     /locus_tag="ACHA5T_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM8886449.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16530..19919)
                     /locus_tag="ACHA5T_000853"
     CDS             complement(16530..19919)
                     /locus_tag="ACHA5T_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM8886450.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20176..21567
                     /locus_tag="ACHA5T_000854"
     CDS             20176..21567
                     /locus_tag="ACHA5T_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8886451.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21640..22287)
                     /locus_tag="ACHA5T_000855"
     CDS             complement(21640..22287)
                     /locus_tag="ACHA5T_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8886452.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22295..22981)
                     /locus_tag="ACHA5T_000856"
     CDS             complement(22295..22981)
                     /locus_tag="ACHA5T_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8886453.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(22987..24060)
                     /locus_tag="ACHA5T_000857"
     CDS             complement(22987..24060)
                     /locus_tag="ACHA5T_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8886454.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24282..24956
                     /locus_tag="ACHA5T_000858"
     CDS             24282..24956
                     /locus_tag="ACHA5T_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8886455.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            24961..26301
                     /locus_tag="ACHA5T_000859"
     CDS             24961..26301
                     /locus_tag="ACHA5T_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFM8886456.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26450..27436)
                     /locus_tag="ACHA5T_000860"
     CDS             complement(26450..27436)
                     /locus_tag="ACHA5T_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFM8886457.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8008 a         5211 c         5005 g         9286 t
ORIGIN      
        1 tagttcatat tcattgtttc ttataataca gtgttcaata cttctcttaa agcacttgtt
       61 gatttgctga acattgcttt ttcttcttcg ttgattgata attcaacaac ttcacgaaca
      121 ccattttcgt caacgattga tggcacacct gtaaagatat ctttttcgtc gtattcacca
      181 gttaaataag cagatacagg caatacagct tgttcgttat tcaagatagc ttttacgata
      241 cgtgcagtac tcataccaat accatagtaa gttgcttttt tacgatcaat gatctcataa
      301 gcagcattac ggactttatc agcaatcaca tctaattcat cttgggcaat acgatggtct
      361 ttttctacaa tttcaaacac tggtttacca ccgacagttg tatgtgacca tgcagcaact
      421 tctgaatcac catgttcacc taagatatag ccgtggacgc tgcgtgggtc aacttttaat
      481 ttcaatgcga tttctttacg gaaacgagtt gtatccaatg ttgttccagt accgataaca
      541 cgtgatgttg gcagaccaga ttcgttccaa gcgatatatg tcaaaatatc tactgggttt
      601 gaagcaacaa cgataatacc gtcgaatcct gatcccatga tttcttttac gatttggcgc
      661 atgattgacg catttgtttt aactaaatct aaacgagatt gaccaggttt ttggttgata
      721 ccagctgtga taacaacgat atcagcatct ttacattctt catagccacc tgaccataca
      781 gccacatttt catcgcccca agccatacca tctaaaaggt ccaatgcttc cccttctgct
      841 ttttcttgat tcacatcgat caatactaat tcattagaga ttccttggtt gatcattgca
      901 taagcgatac ttgttccaac aaaacctgta ccgacgatta ctactttacg acttgttttt
      961 ttcatgaata aacagctcct tttcataact atttgttact ttcatgaaaa gtatagtctt
     1021 gtttattcat tttatcaaca tgttaattat aatagttgtt attataattt ttgatggaaa
     1081 cgaattcctg aatacgcata acagataaat gacaaatgtt tacacctata tcccctgata
     1141 atacaaccaa aagtttagaa ctgaaaataa atccaaaaaa attcgctgca acgatataat
     1201 aagacagcaa tcgcacctaa aacaattttc cttcatatgc tttagtgaaa gattttcttc
     1261 ttaatgttta tttctccatt cattgggagt acatgttgaa tacttcttga ataatttgaa
     1321 taaagaagtc gtttgtttat atccgatacg attagcgatt tcttccaacg aaaaactcgt
     1381 ttcttgaatc agttccaacg caaagtcgaa ttttttcttt agaaccagtt cttgaaagga
     1441 agcatcaaaa tacattttca atttattact aagatagacc ggattgtagc caaaatgagc
     1501 tgctgtttct tttaacgtac aagaagtaaa atgcttatcg atataatcta atactctaag
     1561 catctcattt tctgcgcatc catctgctga tgaaaagctt tgttgttcta ataaatgagt
     1621 caattcgatc agtaatagtg atgtatagag atttaacgaa cggatcttat gactgtatgt
     1681 atcaaaataa atcaacagca tattttcaat caaatgcgtc aaacgacttg tttttccgcc
     1741 gcgaaaatga atgaattgtg tatgttcccc tttttctttt gatgcattta tcaaaaaaga
     1801 agaaagtaga ttatttgtgc ttgccaaatg attcaatatc tctgtagata aggtttcttc
     1861 ttttaataag atgttgatta aaatatcctc ttgccctgtg tattcgattc tttgagtagc
     1921 ttctttatca atgatcacca cgtcactttc ttccatctct atcctttctc cattaataat
     1981 ttgtaaacat gtaccgctat acatataatt gatttcaaca aaatcatgtg tatgctcggg
     2041 aacttccgca tatcgacggt gtttactgat atgaatattt ccctgttgaa atagatcccc
     2101 tcggtggatt tttggcggtg tctgaagatg gtaagctaag tttaaatcat tgatattcat
     2161 tccttgttca cgctgcagag tttcgattga ttcttcgtta cgcaaaaaaa atgtctaatt
     2221 cagctagttc caattgactc cctccttttt gtaagcggtt tctttcgctt attagttcac
     2281 gctgtactgc tattatatgc aaagctattt ttggtttcat aaagactttc tatggttggg
     2341 acatgaactt cctgtgattc cgctattgag agaataacca cttcatacta tatttttgat
     2401 aagcgcttac aaaaatgaaa ggaacatgct tatgaataaa aagatgatga aattttctct
     2461 gctgtcagtc tctcttattt tgacttctgc tggtgcagtc acgtctgcaa ttcccagtat
     2521 gtcagctact tatgccacgc gttctttatc tgctgttgaa ttattaacga ctgctccctc
     2581 ttttatggtc actatttttg ttttgattag tagttttatt gctaggaaaa tcggacaaaa
     2641 acaaactgtg atcatcggat tagtgattgc atcgatttgt ggtatcctcc ccctcttctc
     2701 acaaagttac agtattgtct tagcttcaag agctggacta ggtattggct ttggattgat
     2761 caattctttg gcagtcagca tgattgctgc cttttatcac ggagatgaac gagcctctat
     2821 gattggtttt caaagtgcat ttcaaggatt tggtgcagct ttaatgacat ttgttgcagg
     2881 acagctagta cagttcggtt ggcaatatac ctttttagtc tacgccatca gcttacctat
     2941 tcttgtcttg tttgttttgt ttgttcctac acctgaaaca actgaacttc aatcgcatgg
     3001 cacagcccca acaagacaag taatcaaaac gaatcctaaa atgattagtt atggtatttt
     3061 tctcttgctg gtcatcgtgg tatataatgc tgtatcgatt aaattagcca cagtcctgac
     3121 gacttattct ctaggaaacg aaactaatgc agcgaatatc ttaacaatca tgcaattggc
     3181 aagtatggct gccggagttt gttttggctg gcttcagaca aaagcacatc gctatatgct
     3241 tacactttct ttactactga tgggaatcgg ctttgtattg attgccactg tgataaacct
     3301 gtatgtgatt gggtttggtg cgatcctaac aggaatttct ttttcgttat tcattcctta
     3361 cttattcaac caagtaagta tccaagcgcc caagtacgcg gagagtttca atacttcaat
     3421 cttattggtt ggcgctaatt taggtacttt cattgcacca tatggtttgc tctttctctc
     3481 ttttgcttct cttgggagta aaacacttgc tgttttcgtc aatggcagta tcttgctgtt
     3541 gattagcgcg cttattagcc ttctagtagt acacaggacg aatagaaaga cagtgataag
     3601 agtagatgaa gaaataaaag aacaattgac aacaaaataa atttgatgga ggtcgtgtaa
     3661 aatgacaaat gaaaaacata aagatagatg gctttggtat cctggagact ttgagatccg
     3721 ccatggactg ctacaaaatt tccaacggga agaacgaggt tttgactggc cagcgtactg
     3781 gtacatggat gattgccatc ggaacgtcaa gtttaagaga tattactttc ttgatcaacc
     3841 ttccatgttt aaagtgacga tccaaggagt tgggtatgta gaaattaatg gacaaaaaca
     3901 tccatgtgga aaatggctga cttgtccagc tggtaaagca aaaattcgta tctttgtagg
     3961 acatacatca gggctaccag cgatgtttat cgagggagac gaagtaaaat cagatattgg
     4021 ctggacagca agtaatttta ttgaagaata tccagctggc tggagtccgc tttatgtcga
     4081 tatcgcaaaa gatccaaatc aaatctatta tcaaaaagaa tgcgtccatc cagtcactga
     4141 aacggagatt ggcggaggaa tcttattcga ttttgggcga gcagtgaatg gctgtgtaga
     4201 actaatatct ctggatgacc atatcgaatc tatcactcta tgttatggag agtccgatgt
     4261 agaagctatg gatatcgatt attgctacta taagcaaata ggaatgacaa gaaaaatgaa
     4321 agctcgtaaa cgcgcttttc gctatttgtt tataccagag gtttctactg gaatggttca
     4381 acttcaagtt tttcatgaat ttttatctct ttcgaaacaa gctgcttttt caagcgattc
     4441 ttctttactg aatcagatct ggtcagtatc agaagaagcc ttcaagttat gtagtgattt
     4501 gttttttatc gacggtatca aacgagatcg ctggatttgg tctggagatg cgtatcaatc
     4561 attcatgatc aatcaatatc ttttttttga cgaggaaatc aataaacgaa cgatattagc
     4621 attaagagga caaaatgaaa tcaagcagca tatgaatact atcgttgact attcgatctt
     4681 gtggttgatt ggtatcgaaa accactatat gatgacgaaa gactatgaat ttttacgcat
     4741 cgtctaccct aagatgcaat cattaatgag atacttgatg gagcaaacga atgaattagg
     4801 gtttatttat gggagagcaa aagattggat atttattgat tggtctgaaa tggataaaga
     4861 aggaacgctt gctgcagaac aagtcttatt actgaaagcc tatcattcca tgatcacttg
     4921 cggagatatc ttagctgaaa catatggaga gaacgaaaat tatcatactc atctctatca
     4981 agaaaaatat caactattga aacaaaacct atttgactac ttttgggatg aagaacttgg
     5041 cgcttttatc gattgctatg aatctgggag aagaaatgtg acgcgtcatg cgaatatttt
     5101 cgctgtttta ttcgattttg ctaacaaaga ccaacagcaa tcgatcttaa agaatgtctt
     5161 attgaatgac actatcacac agattactac tccatatttc aaattctatg aacaagatgc
     5221 tttatgcaaa ctcggacaga cgaatatcgt ctaccaaacg attcttgatt attggggagg
     5281 gatgttatct catgatgcgg taactttctg ggaagaatac gaccctactc aaacaggaaa
     5341 tgaaaagtac agtatgtatg gagatcctta tggaaaaagc ttatgccatg catggggagc
     5401 aagcccaatc tatcttttag gacgctattt tatcggtttg tatccaacaa agccaggtta
     5461 tgaagaattt atgatacaac ctaatctaga ttcatttgaa acactgaatt gtacgctacc
     5521 tatcaaagat ggaaagattt cgcttgaaaa aacaaaagat aagatcgtga ttacttctga
     5581 tagaacaggc ggaacagtga aaatgaatgg taaaagttat cctcttctag caaaacagcc
     5641 acttgtctta acaagctatt caaaaataaa tgcataaaac aaaaaactgc ttgcgacata
     5701 tcagaagact ttaaatcttc ttctgatatt gtccaagcaa tttttatttt gaagtttgct
     5761 taattctgtt ctttagaaac aatgtttgtt ctttttccgg caaaaagaaa gctagataat
     5821 acgacaagta aaaagaaagc gatgattcca actgtcaagg ctatttgacc gtattctcct
     5881 agtttcccaa gtacaagtcc tggtactaga taatcagtat cggcaaatgt agaagaagcg
     5941 attttaagat ctcctaatac tggaaggata aataatggca gccaactaat caataagcta
     6001 ttgaaagcag aacctaatac cgcccctctt ctaccgccag tcgagttgcc gaagacacca
     6061 gaagcagctc cgcagaagaa atgtcctact accccaggaa tgataactgt agtttgcaag
     6121 cctatcatag caaacatgga tagtgttcct acaaagaagc taacaaaaaa gccaattaat
     6181 acagcattag gtgcatatgg aaaaacgata ggaacatcta atgctggttt agaatcgggt
     6241 acgagttttt tagcaatccc ttggaatgct ggaacgattt ctgccaacac cattcgaacc
     6301 ccttgaagga cgataacaaa cccagctgtg aatgttccag cttggatcag tgcaaagatt
     6361 acagcgttgt ctccattgct tagttctctt tcaacaaatc caggaccagc taacaaagca
     6421 agtgcgacat atacgatcga catcaagagc atgatcgata cagtagaatc tcgcaaaaag
     6481 ctgaaacttt taggaatctt tatgtcctct gtagaacggt ctttgtttcc aaaaaacttt
     6541 ccgatccatc cactagaagc ataaccgata tttccggtat gccctaacgc aacttgattg
     6601 tttccagtga tgttcttcat aaacggttga caaagagcag gcgtaatagt taatagtgtt
     6661 ccttcgaaga ccccgcctaa taagatcatc cacaaatttg cagtaaaccc cgcgcttact
     6721 aaaatgactg ctgttagaca agaaacataa agcattgctt gacctgtaag aaaaatgaat
     6781 ttatatcttg tgaatctagc aagtaaaata ttcacgatca tccctactag catgatcaaa
     6841 gcagtaggtg taccgtattg ttgcaaagca atcgccacta ctgcttcatt gctaggaact
     6901 actccttgag tatgcagagc tgtttggaac atcgaagcaa aaggggttag tgaattactg
     6961 attaaaccag caccgccgat cagtactagg aatccgacaa atgttttgat agttccttgg
     7021 atcacttctg aaaatttctt cttctgaaga acgagaccaa gcagtgcaat caaagaaacg
     7081 aggatggagg gctgactgac gattgacaca aacaaattca acaatttatc cattgatagt
     7141 tcctcccttg aatcattttt attcttttga caagatcttc gttacttttt cttttacttc
     7201 ttcgctgtca atgatcgatt gcaaaggtac gagcttttca tcaggtatag aagttagcgt
     7261 atctttcaaa gaatgctcaa tgaagaaata atcagccata tcgctagata cacttccaac
     7321 atccgtatga cttacttcaa actcgtccac atctgctcca agatctgcaa gtgcttgttt
     7381 gatgttcatc tctaccataa aacttgttcc taatccactt tgacatactg caaaaatttt
     7441 catatcattc gtctcctttt cttatgatcg ctaatatttc ttcttttgtt gatgcgtgta
     7501 ataaatcttg aagattttgt ttattagaaa gtagctttgt taaatttgcc aaagcccgaa
     7561 gatgtgcctc attatcgata gcagctaaac agatgaacaa actaatggga tgggctggat
     7621 tatcaagcag atccacaggt tgttgaagtt tcagcaaaga catccctact tcgtttacgc
     7681 catcttctgg acgagcatga ggtaatgcga tgtgatcgcc aatatggata aacgcaccat
     7741 actgctccac gcgctcgatc atagctttga tataactttc ttctatttta ttttctgata
     7801 atagcggctg tgcggcaaat gtgattgctt cttgccagtt ttttggttga tccgataatt
     7861 ggatcatttc ttctgttaat aattcagata gcataggacg ttgatcctcc ttcttttcta
     7921 aaagtttgtt cattttacga tttaatactt gatagagctt ttcttcgtct acgccttctt
     7981 ttaattcgat ataaggcagt agcgccgaca acaattcttg ggcagaaggc attgaaaatc
     8041 ctggtatcag ccaatctctt tgtacttgat tgatcagctg atttttttcc aagtcagtca
     8101 tgattggatg cagaacatag agagactttt tcttatcagt aagtgtgaga ggtacagtag
     8161 aaaaaataat atcgtaagaa gtttcgtcaa cctcgtgtaa gcggtcagct gaattagtct
     8221 ctgtaaaaaa gatcatcggg aataattgct gaagttcact ttttaagatc aatgaagagc
     8281 taatgccact tggacagacg atcacggctc ttatttttct atttctttct tctgcgtccg
     8341 cttttctgat ctctccgcca aatagtatag tgaaaaaacc aatctcttct tcagggatgc
     8401 tcttccctat tcgtctttct aacggaagta atgcttttct tgtcatttcg tagatggaag
     8461 catattgatg cttaatttga tcgatcatca cattaggtaa ataaaaagaa taggaaatac
     8521 gaaaataggc agggaccaag tgataaaaga cattcatcaa taattcacga aacgaagcaa
     8581 attctacggc catcaggaat tccatgcgat ggatgacttc tgaagaacat tccagcaaaa
     8641 aatcaagtgc aggctcctgg acatctcctt caacgatcga tgctaagcaa agagagaaaa
     8701 atataatctc cgaattttga tgttcggaat agccaaggcc agtcaatact tgtcgactaa
     8761 tgtcagtgac tgcaagagaa gccaacagtt cagcatcttt taccagttcc atggatgttt
     8821 ttccaattcg aatggttgtc aaactcaaat aataagctaa ttcatccatt cgatttggtg
     8881 cgaatcgaag atctgatagt gaaatcgttt tatctagcaa atctctaact ttggcgtaat
     8941 aatcaatgga aagggttgcg gttaaatacg tgagcccaaa tcttccagca agtgactgca
     9001 tcaaacgagc agtgcgattt tttgcttctc ttcttaatat tgcttctgac cctagcagcc
     9061 taaaaccagt tttcctagta tacgacagct gacaatcttc cacttctaat tcatttctta
     9121 tctttttgat atctgacagg actgtccctt tagaaacctg aagaaaatct tgaaaatgaa
     9181 aaacagaaac agacgttgca gtcgaataaa ccatcaaata aagaagatct cttcgctctt
     9241 gttcggtata cacagcttca aatcgagtaa tatccaaaaa tttttctgcc cagtcgattg
     9301 ctcggaattt ctttatacgg atacgggtat catccacata ttcggcaaaa ggagttaatt
     9361 gtttgatcgc ctcgcttgta tttacttcga tgatctctgc tgctttttct agactgattt
     9421 ctcttggtgt tttcaggaga taactaattt tgtagttcac agcgctgttc acctcctact
     9481 tatattctag tatggtagcg cttttaaatc gaggggtgtt tggtacaact tacagttgga
     9541 ttttttttac gactgtaccg aacgatttct atcgaatgga tacagaaaaa aagcaggtga
     9601 ccaaatgtct cctgctttta tgaattgatt tcgtccgatc aatgatgatc atgtttattt
     9661 tttgaggtca gttcttcaaa tttttctttg acatcttcaa ccgctttttc gatatctttt
     9721 tcaaagtgat tgggctcatt aggctttgtt tcctcttcta cagaatttga accagataaa
     9781 gctgtttctt tattttctgt ttcaccagag tttgtgccag ctaatgattc ttctttgtct
     9841 tctgtttcac cagaattagt tcctgctaaa gccacttctt cttctgtttt aggggccatt
     9901 gaacctctta atcgtttgtg tttttcaccg tcataatttg tcatgttggt tcctcctagt
     9961 agttctatac tttttcatcg tacaataaga cagtttatgc gtcaaaaaac aaacccgatt
    10021 cttacaaata tgtcatattt ttccttctga ttcttgaatg cttttgattc cttcttccaa
    10081 aattttcatt tgtaagattg tttcttcatc tttgatgact ttttctgcgc cattaactaa
    10141 tgtctcgtaa atcccttcgt agacacggct gtaatcacca acctcggaaa ttactttttc
    10201 ttcatgataa ctcccttgtt catctatata ggtcaataca ccataatgtt cgggcaaatc
    10261 gataccaaag tcttcatgat caggcatgta aaacattttc agatgttctt cttgtcgatc
    10321 ttttgtttgt ttcacgaaca tccctttttt tccgtaaacg acgaaacttg gacgttcttt
    10381 gatgcggaaa taactggatt ttatcgaaat tttcatattt ccatagaaaa aatccagatc
    10441 gaagtaatca ttcattcgtc ctttccctaa aagttgacgt acatcatagc tgatatcatc
    10501 tggttcgccg aaataagaaa gaacttgatc tactgtatga caagcatgtc catataaata
    10561 gctgttcgca gtagaaaatt catgcgtatt ttctggtact tccggtctga aataatcata
    10621 atgcatttct acttctaaaa tatcccctaa ttttccgctt tcgattactt tttggactgt
    10681 caagaagtca gaatcgaacc gtctgttttg gtaacattgg ataaatagac ctttttcttt
    10741 tgctaatgca aataactctt ctgcttcctt tgacgtttct gcaaaaggtt tctctactag
    10801 tacattctta ccatgttcga gtgccatttt tccatattca taatgaaatt gactaggtgt
    10861 agtgatgaca actaattgga tctcaggatc atcccaaaga tcagacagct gatcagtata
    10921 ttcgatgtct tcgaatgttt tccacactgg ttcagatggg gaaaaaattt ttttgatatt
    10981 gattttgttc gtaagttttc ttgaaaaagg caaatgatag cgatttgtac tctttccatt
    11041 gccaagataa gcgattgtaa gcatgagtgg tgcctccttt tattgatgtt ttcagtatat
    11101 cctagaaaag cgcagaaagt atttataata gctattctgg taaaaaagtt cagaaaaaca
    11161 cttgttatca ttcggtttgg tgaaattaat gttcaaagta aggataaaaa tcaaaaagat
    11221 agtagcggaa ccaagtgttt atcagtaaga tagaatttgg aggtcagatc atgcttattg
    11281 aagaaaaact aacaaaacaa gagttgttta ccacgacaga gaaacggatt gtcgattaca
    11341 tccgtaggaa tatcgaagct gcagtctaca tgacgattga agaattagca aaagctacgt
    11401 atacttccca ttcagctatc attcgtttat gcaaaaaaat gggattcagc ggatttaaag
    11461 aattccgttt cgagctgggg cgggaagttc atcaattgat tgctcagttc aatcaaacga
    11521 ttgatcctaa ttttcctttt tcaagtgaag atagcactca gcaaattgca aagaaaatgg
    11581 cggatttatc gatccagtca ataaaaaaag cacaattgca gattgaacat gaagatttag
    11641 atcaaatcgg taaagtattg aacaaggcac agcgaatctt tctttttgca aaaggtgatt
    11701 ctcaaatcac ggcaaggaaa ttccagaata aaatggtcaa gttgaataag tttttgatca
    11761 tggctgaaga atacagtgat tcttcttgga atgcagccaa tttgactagc gaggattgcg
    11821 ccatttttat tagctacagc ggtcgcatcc atcattatga acgaataatg acctatttga
    11881 agcatgtaga ggcccctacc ttgctgatta ccggaaatca gcattcagaa atggccaaac
    11941 aggctgatat gtgtcttgtt atatcgcagg aggaatatga ttttgctaaa gtagcgactt
    12001 tctcctctca aattgccttc gattacgtat taaatacgtt attctcagtg atatattctc
    12061 aaaattttga agagaatatc cgcaacttga aaaacaaaca agaattgatg caaaaaggcc
    12121 ttcttaaaga atatcggaaa acttaagaaa agatcctcat ttagtaataa gaaagatagt
    12181 gtttgatagg ctctctttga tatcatcata aaaacaggct gaagttttct ttttcaaacc
    12241 tcagcctgtt tgtttacgga acgattcgaa tagatatatt atagaaatga ggaatagcta
    12301 acttctgttt cttattttga acgattgacg aaaaagatat ataagaagga ggaaaatcag
    12361 tatacatgca gctaacaatc catacaggaa aaatcgtttg ttctcggata ctttctgtac
    12421 ttctttttga acttcttttg catgataagg aacccggtgc cctctgacaa gtaagcgatg
    12481 actgttgacc atataaggtg tacaagttag gagtgtcacg aaatcctgcc ctttttcgat
    12541 tctcagatct tttgtatcag tcggttctac cgtcttgatc tgatcgactt gataagcgag
    12601 tgtccgtcca ttgatttcga tatagaaaga atcacctaaa gtcagtttcg gtaagtctgt
    12661 gaacaacttt gcttgaggca gtcctcgatg gctggaaata acggcatgtg tattttctcc
    12721 ccccgtagga taagacgttc cttccaataa tgaggcacct ttttctagca gcacagtatt
    12781 cgtttcatca aaaattggca aatggacatt gatctcaggg atcgtcaaaa taccgatcgt
    12841 atgtttctca aaataggttc tatccttttt cgcaggttct tttttcttag taaagggatc
    12901 tgcacctgga tttccacctt cttttgctaa ttgctggttt ttctttgtca tgttttcttg
    12961 gattttagcc atgacttctt cattttcttt gaccgcttgt tgctgataat gagagatgat
    13021 ctgctgatcc agataattat tcagcgcgtc gctaacaaat ggataagcta gtatccctat
    13081 tcctgagaga agcaagaaaa gcatcagcag atctagaagt ttccttttct ttggtttcat
    13141 ttgtgttctc cttgtgatga ctgtttgata ataaaagaaa aaggagcatt tgcgtaatcc
    13201 gctgctcctt tttccaattc ttgcaaaaag actaaatctg actgtgcttg cgtctagcaa
    13261 agtacagtcc agcaatcagt agaaggacta ctcctgcacc gatatacaca tagattccct
    13321 taccgcctgt tgaaggaagt gtacctttgt gtttatttgg tattttttca ggagaaatca
    13381 actgtcctgc tgttacataa gattgttcat cgatagtaaa tgctacacgg tttgttaatg
    13441 gaacatattt ttctggcgct ttcgtttctt ccagatagta cgtgccatat tttagacctg
    13501 tcacatcgat taaaccgtta cttgtggttg taaaaaccgt tgctgattct ttcgccgata
    13561 cccagctgac ggcttttgtg gatgggtcga tcgataaata tttcgctgtg tcactatctt
    13621 gatcacgaac gacgaattct gctccagcaa gtgtttggtc tgatgtaacg tcaccatcta
    13681 ctttaacaaa tcgtttgccc ccagtaacga catcgactga cggtggtgtt tgatcatttg
    13741 tatgcccgtt atcgacattt gcttggttag aaaatccttt ggttggatct gctttttcgt
    13801 tcaaatgcat atagtaaacg aatttcaatg taccgccagg agttaatgaa ggaatataat
    13861 tcggatcaac tgaaaccgtg aatccgtctg tttgctcagt gacagaataa tttactgggt
    13921 cgatttcttt atttccatca tataaggcat aagcatacgt tcctgaagaa tcattatcaa
    13981 atgttaaagc agcgtcatga gtatcgataa gtttgaatgt tgtgtacttg ttttgcgttc
    14041 cttctttatc agcgatacct aatgggatat tgacagaaat ctcatattga attttttcac
    14101 cgattgcgac atcttttcca ttcaattgag gtgttgtttt atcaacttta gacgtatcat
    14161 ttttgatggt cttttctact ggtgtttggc tatttgccag gatctcaaaa ggcgtgattg
    14221 tttgcttttc aatcatttcc gcattatcag gagcttttac ttcttctaaa ttatattttc
    14281 caacttctaa atgattaacg atcaactggc ctttttcaat agatgcctcg gcaaagtcat
    14341 tgttgccgat gtcataagaa tgaccagtaa tgaaatgttt ggctttggtt tgatcagttg
    14401 tccaagtgta caatccatct gtgacacttt ggatgtattt tttgacgctt ggtgttcctt
    14461 cttctttaga aataataaat tctgctccat ttagtgcttc gttttcggca gtaccgattt
    14521 ttgtaacttt caacgttcca tcattaccga ctgtattttt agggtagaga tggatagtat
    14581 ctagttcttc tgtcccgtat ttataagagc catctgcttg tttgatcatc tcatatacag
    14641 ggaaagctaa aaccatgttt gcggcagctg acactccgtc ttttggttct tctttgatcg
    14701 tgtagactgc atctttccca ttttgttttt taggtaaaga taaagtgaca tttccatcag
    14761 catctgtcgt tcctgaagca actggtgtac caggagtcaa agactggact gcttgttttg
    14821 ctgcatccac ggacgctcct ttatctcgtt gcgcataaaa ttcttgagtg acgttataaa
    14881 ctgaaaatga aatatcggct aatccttggt attgatcgaa ttcgctcatt tctttcccgc
    14941 tgttttggat taaaggatcg ggtaaatcag tcattttctt tttatgaagg atgacttgag
    15001 ctgctgtctc ctctgcaaac actttttttg cgccgaagct gtttgtgagt aatggtaaaa
    15061 taaggaaaag gactcctaac ataacgttta tttttttatg atttttcatt ttttcactcc
    15121 tgttcttttt ttgataaaca accagattgc aaagcttgcg aataccatac caacaacgcc
    15181 taaaagtgta tttttcgttt cgtttgtttt tggaagaaat ggacggctat tttctttttt
    15241 attgactact ttcaatatac tgtccgcctc tttatcctca gaaagaaaag ttgaaatggt
    15301 aaacggaatt tctgtttcac ttaaaatata accagaaggt gcttcgatct cttttaatcg
    15361 ataacttcct gttttcaacc cggaaattga aaaagcgcca tttttatcgg aaatcaggtg
    15421 aagcgcatct gattcgttct ttgtccactt gtatccgttg gctgtttgct ggaggtaatt
    15481 tcctgctttg tttttaataa ggaatactgc gccagctaat cgtttatctg gtttatttgt
    15541 ctcgattttg acaaattgct tactccctgt atatacagag actttctttg tactgacacc
    15601 aaaaccaaaa tccagtgttc cgttgttgtt gatttccttg ttcgcctgtg ctgtactgct
    15661 tagctgcatc tgataagaaa tggtcagttt ttttccagca tatttttcca acgaagggat
    15721 cgaaaataaa acatcaaatc cgtgaggttt cttgtgtatg acaaagcctt ctgtcagctt
    15781 ttctccattc gatgaaatcg ttaaactatt aggtaaaaac gttaatgctt catccgcact
    15841 atctgacaat tcgaactttt gatagtcaag gatatttaca ggaattgtcg tatgcaagga
    15901 ataagtgatt ttttcaccaa tcgtaaaatc atttctaggc tcttctaata ccttctcaaa
    15961 aggtggatca gggtagtcgt tttcttcatt tttaggatac aaatggatag ttgaaagttt
    16021 ttgattgttt tgtccatgta caggaagaac aactaccata ttctctgcct tttctttgac
    16081 gacttctggt actttggatt caatgaaaag atacgcttta tctctttttt cttgatcttt
    16141 agcggccaga gaaaaagaag cgataccatc ttcgttgtta agagttgtag ttgttgcttc
    16201 tgcggtaaac ataccggaag acgcaccgtt ttttgcgatc tctgcttgtg cttcttctac
    16261 cgttttgccc ttttcccgta gatggtaaaa agaatctgtg acatcataaa cttggaatgt
    16321 gacaccattt aatcctcgat acgtttgtaa taaagctttt tcttgtccgt catttggatg
    16381 atttttcggc agttgcccgt ttgggaaaag caatttgtgt aaagtaatct gaaccatttc
    16441 tgtttctgtt tctgttgcta cctgagtaaa agctggttta aatagtaaca agaagagaca
    16501 catactaagc caaccaagtt ttttcatatt tagctgcccc ctctttcttt tttcaaaaaa
    16561 agataccaaa tagaaaacgc acaaccaatc atccctacta gatagattcc taaacggcca
    16621 attcctcccg tttcaggtaa tggtactttt gcctgattcg tgatgtctaa agaaatctgg
    16681 ttgtttttgg ctcctggtga cagaacagat tcatgatctt gtccatccac ttgaattgac
    16741 ccatcttcgt gtataactat agtaactggc tcttttagac cttggtatcc ttctggtgta
    16801 aaagtttcgg tcagcgtata agttccaggt tttaaatttt cgaatagaaa ggtatctgtt
    16861 tcttgtccat cttttggcga ttctaagtcc atttctggtc cctgcagtct gaattttgct
    16921 ccttttaatg tctgtccgtt gtcttctttt ttgtgtaccg ttagatcaaa cgcttttagt
    16981 cgattttgat gagtgagcgt ccatacatta tttgtcatct cggtattttt tcccgtgtaa
    17041 atgatttttc catatttatc gatttggaat tcataatttc ccggcgaagt atcatatccg
    17101 gcaggaccag ctgtttcaac gactcgatat ttaccaggtg tgagtgaatc aaaactggca
    17161 agaccttttt cattcgttgt ttggctgtca attggctgat aagtaccatt tgcttctttt
    17221 ttctgtaggg aaaatgctgc tcctttaaga tttatctctt tgtccgtccc ttgcatcgca
    17281 tacttacgta ctgctacagg aacttcaaca aaaggatttt caatagtcaa ctggatcgta
    17341 tcatcggaag tagtgactgt ttttccatca atggttaccg taccatcaga agcgatcttg
    17401 atttcccaag tagtcttttt gcttaatcca tgcccttctg gagcttttgt ttctgtcagc
    17461 gtataggtca tttctttttg caatttgaca ttttctggaa gagaataggt tccgtcgcca
    17521 tgatctgtta gtaaagtatc aatagaatct cctgttaatt tgaaaacagc gccaataaga
    17581 tccttttcac ctgtagagga atttttcgtt attttcaagt ttaacggtgt gaattgttta
    17641 gtatttttcc atgtcattgc atctatttgt tgagaatcgt atcctggtac aggtaattct
    17701 ttgattgttt ggtaactgaa tgcttgacct tgattattgt attgaggtag tgataatatc
    17761 tcttgataac tttctccgct atttgcagat aatttgtcaa tgtctgcacg ttcccacgta
    17821 tttgttgtat ctttagctgg tttaatgatt cgaatatatc cgtttttcca agctgcagca
    17881 tttgtcgtat gttcccgttg aatctcaaaa gtaacttgat ctggacgatc agctagattg
    17941 ttgtcaaatt cttcccataa ctttttgatg tgaagactga ctccgggagc tttagcagat
    18001 ggtataccga attcagctaa ttcattggta tcaatgtttg gctggaaagt tgtcctgccg
    18061 ttcatttgat accagaaatt tggatggaag tcctcatttt ctgtttggat tctcacttgg
    18121 taatggattt ggatttcttg gtcttttcct aaatagatct gattgctctt gatggtattt
    18181 ccttctatgg aaatagatgg agtgaccgtt gtaggacttg tccccacact cttgactgaa
    18241 agtgtaccag gctgataaat gaatggttct gcgattggat catttatttg tccattgctt
    18301 acagttgctg agatctgtac agcttttttg gctaggtatt cagagatatc tgttgcatga
    18361 tcagctgatt catagtaaag atcgcctttt tcatctgatg aaaccatttg tcgcatacga
    18421 gactctactt ctgcttttga gagaccagct gccggatcgc tttgaagttg gatgccaaga
    18481 ccatgtattt cgattcctcg ttctttgagt gccatcgctt ctccgatggt tgcgataaac
    18541 gtactgtcga ttcttctgga tagattgttt tggtcaggtg catcatagat tcttgagatt
    18601 agagaagtat ttcccggccg atcttgcgta ttagaaaact gagttccgta ataattgctg
    18661 cttgattgtg cgtgtacccg ctgtacttta taggaaaatg ttggtacacc atccgtcagc
    18721 aaaacgatca cttttttatg tccatttgga acggatagca tgcttcctgc atctcttagt
    18781 gctttttgtg taaaagtacc accatttgtc cgtgaaggtg taatggattt tacttgattt
    18841 ttcactgaat caaatgaacc catctgtact gcaccgttac tgtagctata tccttcgctt
    18901 gagtagccga catatcccat attgattttg tctgtgatac cgctatctgc taaagtatcg
    18961 acaaaacgat cgacaccaat ctttacttca ccgatccgat tattgtcgtt catacttcct
    19021 gaccagtcta cgaccaatac gagatcaaga ggcgtgatat ctttttgtac atttccacga
    19081 gcattcaaat aaacatcaaa caatccgggt gtacttgttt cctttgcata ctttcgtatc
    19141 gcatagtctg cttgattacc ggttcctcca tattcgatat acgaatgggt ccgatcatta
    19201 ggatctccat tccaagaatt tataccatcc cattgatttg ttccgttttt attgccttga
    19261 tgatcaagaa catttgtatt tcctgtaggt tgccaactgg ctttaggata agtgccctga
    19321 tcatctgttg tatattctgt aggaatcgtt tgattagatg gcacactcac agctggcaag
    19381 gataatgtag gcaatgtaaa tgggctgaca gaattttcgt tttcttttga tgattcttct
    19441 tctatttcgt ctggctttgg tactgacaca tctgtatctt tcttattttc tgtctcatca
    19501 ataatgtcat attctttatt atcgacgatc agcttgtttg cctggctgtg gtaaagtgct
    19561 ggatcgattg gaaaactcag atcgatagtt tgtttagtgt ttgatgagat ttccaacgta
    19621 attttcccgc tatttacctg ataagcatag ccatctcctt cttttctttc cacatcactt
    19681 aagtaatttt cgactagacc aatttctttt tctatctttt cagcactggt gttattcgca
    19741 tttagcttga tgttcagtgc tttttttcca gactgatccg aaatgacttc agctgaaatt
    19801 gtttctaaaa ctgttggatc atctgtagtg tctgctattg cagataatgg cacaaagttt
    19861 gataaactca atattatcag catgaaaata actttcagtt tcttccctgt ggtcgtcatt
    19921 tttcccctcc tttttctttt tttctgtttt gacgtgaata gtgaaaatta ttaaaataaa
    19981 caggcgttac ataattttat ttattttaat atgataatta ttatacgaag ggcgctaaag
    20041 ggaatcacac caattttcat accaattggt ttcatttatg aagttgattc cttcgattaa
    20101 tgagcgtttc attgaaagaa aagggttgag ggagaaaaac atatgatcag gttgttggaa
    20161 ttaaactata gaaagatgga aaaaatcatt cgaattctgt ccatttctga caagtggatg
    20221 actaaaaaag aacttgccaa acgaattggt agttcagaat ctacatttgt aaggtatatc
    20281 gaagagatca aaatccgctg gggaacggat gtaacgattc aatcttcaca aaaattgggg
    20341 tatcgtcttg aacatttgaa tgtaccagct tatttaaatg ttctgatgga aatgtcgcaa
    20401 agttctacaa acagtcgttt attaaatgag attattgtca atccgggtaa aacgattgag
    20461 tattattgtg aaaaaatttc catcagccgg tcttctttta cacgtaagtt gaaacaatgc
    20521 aatcaaattt tagaaagcta tttattaaaa gtgatcgttg accaaggttt tcagttaact
    20581 agccttaaaa gtgagctcca gttgcgtatc tttgttacct ttttcttttt aagttactac
    20641 ggaagacgca cgttgccatt tccaatcgaa aaaaagaaga tcaaagaaat tatggtcaag
    20701 aataattgtc agatgaattt gtttcaggaa gaaagcccat atgagcaatc tttctttatt
    20761 atgtattttg ttgtgcatct catgagagaa tcccaaggaa ctccatttga atggaaagct
    20821 ggagactctt atgtacagtt gataaatatc gataaaaaag acttcttaat cttgaaaaat
    20881 ctcttaggaa aattgaagta cagcagctat ataaaagtag tgaactattt tgtcaatacg
    20941 attttttcag aattttcgga gaaagaaaaa aatgaaatca gtgagcagat acgaaaaaat
    21001 atgtcctact gttcgttttc atccgctgat tgcaaagaaa aaaacgaaga atatgttgtt
    21061 caattattaa cgcaaattta ttttttctcc cgtattttcc catacgatac gactcatttg
    21121 acgatccgtg caacttactt tgcacagcag atgcaacacc gccagatacg tctatatgct
    21181 ggatttcgtg cagaacttca attttattcc actctactga atcagaatct gatttattat
    21241 ttatctagct atatcttttg gatgcttatc ggtaatcctg aaattcacca ttatacttct
    21301 gataaaaaaa tactgattat cagcgactta agtcttcgac atagtcaata tattgaagaa
    21361 tatatcagtg atattcttgc tgtgcataaa attcactctg agacaactgc tattactgaa
    21421 gatcagctgt cgaaatacaa tcttctcgag tatgatctga tcgttacgaa tcaacccatt
    21481 ttgaatgccc atgttcctca tatattgatt gatgatagtg tgtcattcgc aaatgaagaa
    21541 gaactcatcc gcttattcga gttatgaata atcaaaaaac agctatgtat aaaccaccct
    21601 gataaaaatt tcaggacaaa tacacatagc tgtttctttt tattgtattc cttcagcaat
    21661 ccaacgagat ttatagcgaa agtcttttaa caactcttct tttgtccaat tatttgcaat
    21721 gagatccgtc aaagtgttca ttacgagggt catggaaaag tataggaaac taactgcttc
    21781 ttccgacgat tgaatggaga aatgattgat tcttaataat tccagccatt tttcgttcat
    21841 tggagaatca gccgatagtt ctctacgttt ggttgtctgc tgatcatttc gctgggttaa
    21901 caagtataac ctttgccaat agatactttc gtgatctaag tcgcagcacc actctaaata
    21961 tttttcgatc cccaaaaaga aatgttgttt gttttcgcta atgaatttta agatatcttg
    22021 atggactatg tgagaactgc ttttgatcgt atacaaataa gcatcttcca aatcttgaaa
    22081 atatttataa aaagcaccgc gcgacatccc catcttgtca acgatttctg agactttgac
    22141 ttggctgatg tgctgtgagc aaaatatctc cagtaatagg tcatcgattc tctgtttctt
    22201 tgcgttcgat aaattgaaaa aagtttcttt tggcatccac atctctcctt tatctctgat
    22261 tatacgtgga tagcccttca ttgtcatgaa ggattcattc cttctttttc tcttcttctg
    22321 tcaagtaccc atcttccatt cgataaacgc gatcactcca ttgaatcata cggacatcat
    22381 gagtaaccat aatagtcgct ttttgtttct catgagcttc tttaactagt aatttgacga
    22441 cttcatatgc atgatctgta tccaaactag ctgttggttc gtctgctaag atcaagcttg
    22501 gctcattata taaagcgcga gcaattgcca ctctctgtct ttctccacca gaaaggtctt
    22561 taggataact atttttaagt gactggatat ctaaagattg cagcaaggaa tcgatttttg
    22621 ttgaatcttc tttttttgaa gtaacttggt caactagttc aaattgttcc tttaccgtta
    22681 aaaaaggaat caaattagag ctttggagaa taaatcctat ttctttaaag cgtaaatctg
    22741 ctcgtttttt ttctggaagc tcagttagtg agtgaccgtt gatcactatt tctccggaag
    22801 tcggtgtttg caatcctcct gaaatggtaa ggaaagtact tttacctgaa ccagaaggac
    22861 caatgatgct gacgaattct ccttccttta cataaaaatc aattgctttc aaagctgaga
    22921 cctttgtatg accagaacca aatgttttgg taactttttt catcgttaat atttttttca
    22981 tttttattaa cctcctattg ctgtaattgg gtctactttg gttatcgtac gtatcgaaaa
    23041 caatccgcct agcattgcga caatgatcaa aatcccacta tatagcaacc attgtgacca
    23101 aaaaacagca aaaggcatcg cgcttggtaa aatcagacta gtcaaataag caaacaaaag
    23161 agcaagcaga acgcctacca ccccaacaat aaaggactgt gctactaaag atttagcgat
    23221 gaagaaattt ttgatccctt gtgctttcat tactccaaaa atagcagtct tctgaagtgt
    23281 catcacgtac atgaaaatac cgaccacggc agcaaccact aagaacaaaa agtatatcat
    23341 cgcatttaag gtcatatttt gggcagaata gccaggaata ctttcaatga atgtcccaat
    23401 agttaataac tgtagaccct tcgatgcttt ttctttcgaa attttagctg aatcctttat
    23461 tacgatccca ttgatcggct gttcttcttc agaagcaaac ggctgttgtc catacttcaa
    23521 agcagtccac gtatctaggg acgcatagat gacgggagaa actgtgtaat aagtttcagg
    23581 aaagataccg acaatcgtca aaggatcgtc gtaactgcca attttgatcg tatctccaat
    23641 tttatagcct gaatcagcca gattttgaga gatgatgatt tcattctttg ttttaaattc
    23701 atgccctttc gtgagttttg gtaataaaaa tgcttctttc gttgtgccga aaactgtaac
    23761 attttctttg ttttttcctt tgattgctcc gctgtacaat ccgattggtg ctttctctcc
    23821 tccttcaatg tatttcagat ctcctcttgt taattgtgaa gctgcaaata cttgatttgc
    23881 atcctccgac aaaacaatct cttgtgcgtc ccaatcatcg attgcttttt taaactcttc
    23941 tgaaagtcca ttcgctagtc ctgacaacat gaatacgaca tacgcaatca agaacataat
    24001 accgacgatc aagccatatc gtaatttaga atagcgaatt tctttgatag ctagaaacat
    24061 ttgtttttcc tccctatgat agattgatag tgacaaaatg tcacttgttg aatagtgtaa
    24121 ctctaaagtg acaatttgtc acttgtgttg tttttttctt atcaataaat tgtctaagac
    24181 atctttcgat aaaaccttaa catgagtcat aaaaaattta tttaagcctt atttatgctt
    24241 tccatgataa actaaagtga ccaaatcaat gaggtgggaa aatgattaaa ctattagtag
    24301 tagaagacga taaagtatta tccaataata taaaagaaat actcgctgca atcggcgaga
    24361 taacacaagt ttatgatggt agtgaaggac tgttcgagtt ggaaagtgga gtttatgatc
    24421 ttgcaatctt ggatttgatg atgccggaaa tgaatggata tgaagtgtta gcaaaaatac
    24481 ggaaagaaaa aatacagact ccagtattga ttttgactgc aaaagatagt ttggaagata
    24541 agattgaagg atttgaacga ggtgcagatg attatctaac aaaacctttc tatcgagaag
    24601 aactgatcat gcgcgtgaaa gcattattga aacgctcgct aggtctcttt gatgaaaatg
    24661 ttctgaagta taaggatatc agttgtgatt taaaaagcaa tgaagtcttg tataaagaac
    24721 aagttttgcc catacaagga aaagagtttg aattgctcgt ttatttttta caaaataagg
    24781 gaatcatttt gacaaaagag cagatatttg accggatctg gggatttgat tctgatacga
    24841 cgattacagt tattgaagtt tatatgagca acttacgaaa acatctgcga agtactggat
    24901 tggataaaga aatcaaaacc ttacgtaatg tcggttatat actgcaaagg gtgtgatgca
    24961 atgaatggag atattacacg aaagcaacgt agtcgctttt ttgcgacaaa tgtagcagca
    25021 tttgctttta tcttttttct tttgggattc attactttac agatattaaa ccaatctgca
    25081 tacagagaaa cagatctgtc attagagaaa ataaattttg attctcgaac agtacaatta
    25141 gagatacaaa gatatcagca aggcgatccc tttttaaacc cgactcaaac ggaacaaaac
    25201 agaacaaaca tggaacagcc attaatggaa ccatcaaatg accgttttaa cactcaagtg
    25261 atcttgtggt ctaaaaatgg tgagatattg aataaagcag ctattggtgg gcgtttcaac
    25321 caaatcaaag aattaaaaat ggatacgaaa aatctgaata ccatccaaaa tattgaatta
    25381 aatgaagaca gtggctcatc tgccctctct ttccgttcga ttacgaaaaa agcggagacg
    25441 aatcaagatg gcgttgccta catccaaatt ttagaaaata caaatcaaat caagaattca
    25501 ttagcgactt ttcgcacgat tatgattttg tgtatgattg ttttttggat tctctctatt
    25561 ggtatcagtt attacttgtc tagtctaagc atgcgcccta ttattctttc ttggagaaga
    25621 caaaaagaat ttgttgagaa tgcttctcat gaattgcgca caccgttaac gattattcaa
    25681 aatagtttgg aacatctttt taccaagcct aatcatacta ttattgaaga atccgaaagc
    25741 atcgctcaag cattgtcaga gacgaggcgc ctcactggat tgacttcgga tttgctgaca
    25801 atcgctcgga atgattctga tcagcagctg ctttcaaagc aaatgatcaa tacacaagaa
    25861 tatatcgaac aacttgttaa accatttcaa gagatggcca tcatagatgg aaaagagttt
    25921 attctccata acgatgcaac attagaagta ccaattgatg aaaaaaaaat ccatcaagtt
    25981 ttggttattt tgctggataa tgcattaaaa tatacgaatc cagaagataa gatcattttg
    26041 gattcatctg ttaaccataa agaatggctg attcacgtaa agaatactgg cccttctatc
    26101 agtgaagaag atcaagcaag gatttttgat cgattttatc gggaagatca atctcgggcg
    26161 aaagagactg gcggatacgg gcttggcttg gcaattgcaa aacaggtaat cgaacagcat
    26221 catggacaga tttctgtatc caattatctt ccacaaggag tcgattttaa agtccaattg
    26281 ccgttgaagc ctaaaaaata atgcttatcc ctaaaaaagg gctgtgacaa aaatcttgtc
    26341 acagcccttt tttgttgcga ataaacggtg ttccagaagt agcttcttcg gaaataagcc
    26401 gaaatttctt taagctgacc acttctgtca caacctcttt agcttacgct tacgattttt
    26461 tattgcggtc ttcaatgtgt acatcaccgc ttgtgtcaat atccaattct tctcggctaa
    26521 catcttctgt cactttttca acatcttcat gttcttcttt atgaatccca acttcttcac
    26581 gaataactgt atgtttgctt acatctactt gctcttcttt gattgggata gtaatcgtgt
    26641 catcttccaa tttttcgtct gttccctgtg aagattgatc tgttactggt ttgcgttcga
    26701 tgacgatttc ttcacgtttg accggtactt ctaccgtttc agtatcgttg accacatgtt
    26761 tgtggatgtc gacttctcca gttgtcacat catgtgtatt tacatctaat ttttcttctt
    26821 ttagttgaat tgtatcatca tctagatttg gtttgcgatc gatgtcttca gtcttgcttg
    26881 tacccattgt atcagtctcg ccaggtacgt ccatcggatc agttcctggt gtcatattca
    26941 atggatccgt tgtattttga aagcctgtcg tgccacctac gtccattgga tctgttccag
    27001 tgccagtttc taatccagtc cccattgtgc ttgctgggct aacagtatct tgtgtatcca
    27061 ttgtatcttc tgttgtttct ggctgacgat agccttttac tgtgatgaca aattttccat
    27121 cagaaatatc tcctctatat tgatataaag gatcattttc tggtgtagcc gtctcagaat
    27181 cataggtatc aaaagaaaaa gtatctttta ttttttccca taaagaacgg tcttcttcac
    27241 catccatccg attttcatct gtttcaacat ctataccaga caatccgtca aatcctagtc
    27301 tagatgctgc ttcatcagta gtataaataa cgatatcatc tcgttcatac ccttcgttac
    27361 gcaaccgttc aattactgat aatgcttctt ccctcgtact atagcttccg atgaccgtta
    27421 aatttttctc ttccatgtaa attcctccta gcaaatagat ttatgattgg tatattgtta
    27481 cttataaatt tattctatag gagacgattg
//
LOCUS       DAYYGO010000023        27248 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000023 DAYYGO010000000
VERSION     DAYYGO010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.023"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(113..307)
                     /gene="ssrS"
                     /locus_tag="ACHA5T_000861"
     ncRNA           complement(113..307)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHA5T_000861"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(379..1665)
                     /locus_tag="ACHA5T_000862"
     CDS             complement(379..1665)
                     /locus_tag="ACHA5T_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFM8886458.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            1803..2474
                     /locus_tag="ACHA5T_000863"
     CDS             1803..2474
                     /locus_tag="ACHA5T_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFM8886459.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            2440..2928
                     /locus_tag="ACHA5T_000864"
     CDS             2440..2928
                     /locus_tag="ACHA5T_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFM8886460.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            2941..3888
                     /gene="prmA"
                     /locus_tag="ACHA5T_000865"
     CDS             2941..3888
                     /gene="prmA"
                     /locus_tag="ACHA5T_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFM8886461.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            3889..4653
                     /locus_tag="ACHA5T_000866"
     CDS             3889..4653
                     /locus_tag="ACHA5T_000866"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFM8886462.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            4886..7099
                     /locus_tag="ACHA5T_000867"
     CDS             4886..7099
                     /locus_tag="ACHA5T_000867"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFM8886463.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            7110..7556
                     /gene="dtd"
                     /locus_tag="ACHA5T_000868"
     CDS             7110..7556
                     /gene="dtd"
                     /locus_tag="ACHA5T_000868"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFM8886464.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            7727..8986
                     /locus_tag="ACHA5T_000869"
     CDS             7727..8986
                     /locus_tag="ACHA5T_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFM8886465.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(9075..10022)
                     /locus_tag="ACHA5T_000870"
     CDS             complement(9075..10022)
                     /locus_tag="ACHA5T_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM8886466.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            10416..11708
                     /gene="hisS"
                     /locus_tag="ACHA5T_000871"
     CDS             10416..11708
                     /gene="hisS"
                     /locus_tag="ACHA5T_000871"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFM8886467.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            11723..13492
                     /gene="aspS"
                     /locus_tag="ACHA5T_000872"
     CDS             11723..13492
                     /gene="aspS"
                     /locus_tag="ACHA5T_000872"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFM8886468.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            13601..14476
                     /locus_tag="ACHA5T_000873"
     CDS             13601..14476
                     /locus_tag="ACHA5T_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8886469.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            14742..16625
                     /locus_tag="ACHA5T_000874"
     CDS             14742..16625
                     /locus_tag="ACHA5T_000874"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFM8886470.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(16700..16984)
                     /locus_tag="ACHA5T_000875"
     CDS             complement(16700..16984)
                     /locus_tag="ACHA5T_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM8886471.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(16974..17708)
                     /locus_tag="ACHA5T_000876"
     CDS             complement(16974..17708)
                     /locus_tag="ACHA5T_000876"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFM8886472.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            17837..18460
                     /locus_tag="ACHA5T_000877"
     CDS             17837..18460
                     /locus_tag="ACHA5T_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFM8886473.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            18652..19773
                     /locus_tag="ACHA5T_000878"
     CDS             18652..19773
                     /locus_tag="ACHA5T_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFM8886474.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            19780..22908
                     /locus_tag="ACHA5T_000879"
     CDS             19780..22908
                     /locus_tag="ACHA5T_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8886475.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            22927..26121
                     /locus_tag="ACHA5T_000880"
     CDS             22927..26121
                     /locus_tag="ACHA5T_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFM8886476.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(26267..26974)
                     /locus_tag="ACHA5T_000881"
     CDS             complement(26267..26974)
                     /locus_tag="ACHA5T_000881"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8886477.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT         9096 a         4854 c         5832 g         7466 t
ORIGIN      
        1 actaaagtgg cgataatcat taaaatccta atcatgaaca aatcctccaa aataagcaac
       61 aaatagaaaa agcaatttgc caagagcaaa ttgctttatt ctttaaaaga tggtaatctc
      121 cgttaatgcc gttgtttgtc ctgtagtatt gatcccgcga attcaagcag gtgggtccca
      181 tggtcgtagc ttcgaactac caaatgataa ggcatgttac cagcagtgac acaagacggg
      241 tcccaagata tcaataaaaa tgttcggtca acacacaaag aggacaattc gaacatcaca
      301 gatttcccac ctctatagta gcatatgaat ttaaaaagag caatgagaga gctcgcagaa
      361 acagaaccgt tttcttgtct agttttttgg aggatgcttt ttccactctt ttatacggta
      421 atactgttga cccagagcct gttcatattt ccctgtatct tttggctgat aatactgagc
      481 attcttcagt ttatctggta aatattgctg attgacccaa gcctgatcaa aatgatgagg
      541 atactgataa cccacgccac ggttgagtga tttcgcacct ttgtaatggc tatctctcaa
      601 atgatcagga acatctccag ctttgccctc tcgaatatca gcaagtgctg catctaatgc
      661 catgtaggca gaattggatt ttggcgacaa gcaaagatcg acgacgacgt ctgctagtgg
      721 gatacgtgcc tcaggcaatc ccaatttttc agcagctaat acagcgttca ctgtacgagc
      781 cgcagcagca ggattaccta aacctatatc ttcataaccg ataaccatca gacgccggca
      841 aatacttgcc agatcacctg cctcaactaa acgggcgaga taatgaagtg cagcatccac
      901 atcacttcca cgaatcgatt tttgaaaagc cgaaataacg tcataatgag catctccatt
      961 tttgtcatgg gtaagagctt ttctttggac acattcttca atgatcgaca gtgtaagatg
     1021 aatccttcct tctttatctg aaggagtcga tagtgtagcc agttccagcc cattcaatgc
     1081 actccgcaaa tcgccgttcg ttgcacgcga aaggtgaaga agagcatctt cgtctaactg
     1141 gattgcttgt tgtccaaggc cgcgttcttt atcccttaaa gcatgttcga ctgccaactg
     1201 gatgtcttgt tctgttaatg gtttgacttc aaagatctgt gtacggctgc ggatcgctgg
     1261 attgatggta atataaggat tctcagtagt cgctccgatc aagatgattc ggccactttc
     1321 taaatgagga agcagaaaat cctgttttgt tttatctaat cggtgtactt catccaataa
     1381 gaggatcact gtaccactca tttttgcttc ttccgctaca acttgtagat ccttttttga
     1441 atctgtagcg gcgtttagca tgcgaaatgc ataattagta gagccggcga tcgcacttgc
     1501 gatactcgtt tttccagtac caggaggtcc atatagaatc atggaagaga gcatgcgagc
     1561 atcgaccatg cggcgtatga tttttccttc cccgactaga tgctgctgac caacgacttc
     1621 atcgatcgtt cgaggtctca tgcgataagc caatggctgt tgcataggat tttcctcctt
     1681 cttgttttta cgtcccttaa ttatatcata tttagcaaga tgcctagaaa gaacagcctt
     1741 ctgttgtttt gttttcatag cttatccaac taatattgtg tatgataaag taggtgagaa
     1801 aaatgacgga taaacaaaag atagatgaat ttttttggaa taaaacgacg ccagaagtgg
     1861 cacgggattt attggggatg tatttggagc atgacacacc agaaggaaga ttagctggat
     1921 atattgtaga cgcggaagca tacctaggtc cagaagacga ggccgcccat agctatggtt
     1981 taagaagaac accgcgtgtg cgggcaatgt atgaaaaacc aggtacgatc tacctttaca
     2041 cgatgcatac tcatcggatt ttgaacatca ttactcagcc agaagggatt cctcaaggag
     2101 taatgatacg tgcaatcgaa cccgccgcaa tgattgatca aatgagtaaa aatcgtggcg
     2161 gaaaaactgg tccagatatc agcaacggtc cgggaaaatt agtggaagca ttagcaattc
     2221 ctcaagaact ttatggtcag tctatagcag atagttcgtt acgcttagtt tttgagaaaa
     2281 aaaagacacc aaaaaagatc aatgctttgc ctagaatcgg tatcccaaac aaaggtgtat
     2341 ggacagaaaa gccgttgcgt tttgttgtgt cgggaaatcc ttatatctct ttacaaagaa
     2401 aaaaccaaat cgagaaagat tggggttgga ggaaagaaaa tgaaaaagaa ggatctatta
     2461 acatatttag atgaaataat tgaaaaacaa gtgacagact atgatgtagc tttagattgg
     2521 gatacaaaaa atcatacgat cgagatcgtt gttcgcttgt ttgcagaaaa taaagcacaa
     2581 tttgagattg atgatgcaga agggatcgtt tcgcaagaac caattatcga atttgaagac
     2641 ggcgtacttc tttttaatcc tcagaaatct gtttttgatg aacgcaatta tctagcagta
     2701 atcccttatg aaggaaaaaa agggttggca aaatcagtag ctgattcttt agtggaatat
     2761 ctaaatgaag tattagctca agggcaaagc gatttgcttg attttttgga tgaagacgat
     2821 gacgaagctg tattcgagct tcactgggat aatcagcaac tagcagaatt agtagaagta
     2881 aaacagcaaa atgaagcaga tacttattta ccgtacccaa gttattaagg agtgacagat
     2941 atgaaatgga atgaagtaaa agtagaaact gaaagtgaag cagtcgaagc agttgctaat
     3001 attttgatgg aggcaggggc gagtggtgtc gcgattgaag attctttaga tgttgaacat
     3061 tttcagtcgg atcaatatgg tgagatttta gataaaaatg attttacatc gctgaaagaa
     3121 ggcgcactag tcatggctta ttttccggaa acgactttct taccggaaat cttgcctttt
     3181 atcaaagatc gtatcacaca attacctgag tttggtctag ctattggaaa aaataaagta
     3241 acagttagtg aagtagagga aagcaactgg gcaacagcct ggaagaaata ctatcatccg
     3301 gtgcgtatta cgcgaatgct gacggttgcg ccaagctggg aaaactacca gacagaagat
     3361 cctctagaaa aaattatcac actagatcct ggtatggcat ttggcacagg cactcatccc
     3421 acgacttcct tgacattgac agcactagaa acagtcttgc gtggtggtga aacattgcta
     3481 gatgtgggaa ctgggtcggg cgtgctaagt attgcagcaa aatatcttgg tgcaaaagat
     3541 gtctatgcct ttgatttaga cgaagtagcc gttcgttcgg cgaaagaaaa tatggatatg
     3601 aatgaaatag ccaaagatgt ccatgtatca gctaacgatc ttttgacagg aatcgagatt
     3661 gaaagtgatg taatcgttgc gaacatttta gcagatatca tcttattgat gatcccagat
     3721 gcttggcgtt tactgaagca aacaggcacg ctgatcgttt caggaatcat tgaagagaag
     3781 aagcaaatgg ttctggatgc catgttagaa caagggttta tcgtggatca aattttccag
     3841 caaaaagact ggtatgcaat tattttgaaa aaaccagagg tggaatagat gcagcggtat
     3901 tttttaaaag aatcatatga atcaaaagac tattttgttt tagaagggga aaactatcac
     3961 cacattgtac gggttatgag gatggaaccc gaggatgaag tttttttggc attcaatgat
     4021 caaatagcca ttgttgcaga aatcacggag attactgaag aggctgttta tctgaaagaa
     4081 attaaaaaag aagaaaatca aaaggaactg ccaagttcga ttaccatagc gagtggctat
     4141 cctaaagggg aaaagctgga ttggatcgtt cagaaatcca ctgaattagg agcgcaagct
     4201 tttcttggtt tcccggcatc agcctctgtc gttaaatggg atgcaaaaaa acgtatgaaa
     4261 cgacaacaac ggcttgaaaa gattgctcaa gaggcagcgg aacaatccca tcggcagatc
     4321 gtgccatcag tagagttatt ggaaacgaaa caagatttat ttgagcggct agatgattat
     4381 gacgtggtct tggttgctta tgaggaatca gcaaagcaag gcgaaaaatc aaatgcagcc
     4441 aaaattctta gtgaaacaaa accagatagt cgaatcttag ctgtctttgg accagaaggt
     4501 ggattatccc gcgatgaagt cgattatttt gtagaacaca aagggatact ttgtggatta
     4561 ggaccaagaa tccttcgtac agaaacagca cctctttatt tattaagtgc tgctagctat
     4621 caatgggaat taatgaatcc ttctttcagc taacgttctt cattaataaa agagtatcga
     4681 cagaaagaat tgaaaaaata gtcaacaatt tgtataatag aaacaattat ttatacaatg
     4741 gatacgaaaa atcgtttgaa aaagctttta aaaagcgaac atttccgtaa aaaaacgtat
     4801 aactgcacgg attttgggac aattatcaaa cagatatttg tcccgattcc cgttttaccg
     4861 aaaaaagaaa gggggatcga ttcctgtgac aaaagaagaa attttaacgg gacccggtgt
     4921 cataaagctc gttagtcaat acatgggtcc cgaacatgta gcatttgttg agaaagcatg
     4981 cgaatacgcg actgctgcac atgacggaca gtttagaaaa tcaggcgaac cctacattat
     5041 tcatcctatc caagtcgcag gtatattagc agatttaaaa atggatcccc atacagtggc
     5101 tacaggcttc ttacatgatg ttgttgaaga tacagaaatc actttagaag atctgagaga
     5161 agaatttggc gatgacgttg ctatgttagt ggacggcgta accaaattag ggaaaataaa
     5221 atataaatcc cacgaagagc agctagcaga aaaccaccga aagatgctgc tagcaatggc
     5281 tcaagattta cgagttatca tggtcaaatt agctgataga ctacataaca tgcgtacgtt
     5341 gaagcacctg cgagaagata agcaaagaag gatcgctcag gaaactttag aaatctatgc
     5401 gcctcttgca catcgtctag ggatcagccg gatcaaatgg gaattagaag atacagcact
     5461 tcgttatcta aatccaaaac agtattaccg tatcgtccac ttgatgcaga cgaagagaga
     5521 agaacgtgaa aaatacgtaa gtggtactgt tgaagatatt cgaatagcga cagaagagct
     5581 ggggattttt gcagaaatct atggacggcc aaaacacatt tattcaatct atcgtaaaat
     5641 gaaagatcag aaaaagcagt tcaacgaaat ttatgacttg ctagctattc gagtgatcgt
     5701 agattcaatc aaggattgtt atgctgtatt aggagcaatc cacacaaaat ggaaaccaat
     5761 gcccggcaga tttaaggatt atattgccat gccaaaagct aatatgtacc aatctttgca
     5821 tactaccgtg atcggtccgg caggaaatcc ggtagaaatt caaatccgaa cacaggaaat
     5881 gcatgaaatc gctgaattcg gggttgctgc acactgggcc tataaagaag gaaaaaatga
     5941 aaaagtagaa ccagatggta tgacgaaaca attaagctgg ttccatgaga tactcgaact
     6001 tcaagacgaa agctatgatg cttctgaatt tatggaaggc gtaaaaggag atatctttag
     6061 tgataaagtc tacgtcttca caccaaaagg agacgttact gagttaccaa aaggatccgg
     6121 accattggac tttgcataca gtatccatac agatatcggt aacaaaacca ctggtgcaaa
     6181 agtaaatggc aaaatggtgc agcttgatta caaattgaaa aacggagata tcattgagat
     6241 catgacttct ccaaattcat ttggcccaag tcgcgactgg ttgaaattag ttgctactag
     6301 caaagcaaga aataagatca aacgtttctt caaagcccaa gatcgagaag aaaatgtgat
     6361 caaaggccac gaatccgtgg tcaaatgtat tacagatcta ggatttacgc ctaaagatat
     6421 tttgacgaag aacaaactgc aagaagcact cgatcgtttt aattatcaaa cagaagatga
     6481 tctctatgca gctgtagggt atggagaagt tagccccttg acgatggcca atcgtctgac
     6541 tgaaaaagaa cgtaaagaac aaaaaatcga gcagcaaaag caagaagcag aagaaatcat
     6601 gaatcagccg aaaaaagaac ctgacaaaat gaaagtacgt catgaaggtg gcgttgtcat
     6661 tcaaggggta gaaaacttac taattcgtat cagtcgctgc tgtaatccga ttcctggtga
     6721 tgatatcgtt ggttatatca ctaaaggcag agggatatcc attcatcgtc gagattgtcc
     6781 gaatgttcag cctgacaaac caaatgtagc agaacgtttg attgaagtcg aatgggaaga
     6841 tacatcgaat acacgaaaag agtatgatgc agatttggaa atttacggct ataatcgttc
     6901 aggcttattg aatgatgtac ttcaaacagt caatgcgcta acgaaaaatc tcaacagcgt
     6961 tgaagcacgg acgaataaag ataaaatggc gacgatccat ttgacggttg gtatccagaa
     7021 tttatcccac ctaaagagta tcgtggataa aatcaaagca gtacctgatg tctacagtgt
     7081 acgccggacg aatggatagg aggacagata tgagagcagt tgttcaacgg gtaagtaaag
     7141 ctgctgtgtc agtcgatcag caagaaattg gaaaaattgg ctacggccta cttgttttat
     7201 taggcattca tgaaaccgat acgcaaaaag atgtggacta tctggtaaaa aaaattgcac
     7261 aaatgcgtat ttttgaagat gaacaaggaa aaatgaatat tagtgtagaa gatgtgggag
     7321 gacaaatttt aagtatctct caattcacac tgtttgcgga tacgaaaaaa ggaaatcgac
     7381 caagctttgt ggaagccgca cgaccagaaa cagctattcc tttatacgaa tcattcaatg
     7441 aaggaatcag aagtcgcggc atcacagtcg ctaccggaca attcggagca gatatgtcgg
     7501 tttctctcgt caatgatgga cctgtaacga tcattattga ttctaaaaac aaataaagta
     7561 atctggtttt cactagtaaa aactatgaaa cacgtcgctt aagttagact gataaactct
     7621 aacttaagtg gcttttttgt ctgcaaaaag cgcttctttt gtcagcagtc ggaaaaaaag
     7681 ttaagcttaa attatacaag ttatttttta taggaggttg ctgaagatga aagaagtacc
     7741 agtagtagat atcaaaatca cagatttaaa aaaattgtat caaaaaggat acaacatgtt
     7801 ggaggagtta cgccatgaag cggacgctcc cgtcgtcaaa gcaaagattt tcaataaaga
     7861 agctatcacg atctacggat catcagcagc aaaagtattt tatgatccaa ggaatttcaa
     7921 acgaaaaggt gctatgccaa aattagtatt gaagacactt tttggtcaag gtggagtaca
     7981 gactttggat ggagctgctc atcaccatcg gaaaaatatt tttatggatc taatgacacc
     8041 ggaaagaatg gaagattatc atcgtatctt agataagaat ctgacacaag cacttgaagc
     8101 acagcatggt caatttgaac tatttgattt aagtaagatg gttttcttta catctatttg
     8161 cgagtgggca ggtatcaatt tatcagcgat ttctaaagat gaggtcgaaa aattagcaga
     8221 atatcaaatc tctatgatca gcggaacgtt tacttctccg attgatcaca taaaaggtgt
     8281 cgagaatcgc aaaaaatcag aaaaatgggc gcaaggcttg attgaagaag ctcgacaaaa
     8341 tcctgtggct ggaaaagaaa atgtggcatt atatgctttt gccaatgcca cagatttaga
     8401 tggtcagctt ttacctctgg aagtggcagc tgttgaatta ttgaacatca tccgtccaac
     8461 ggttgctcta actgtgtggg cggcattgat gggacatgca cttttcagta gaccagattt
     8521 gtatcagcaa ttaaaaaatg acttttcgac gttacaagat ccgtttattc aagaaatgag
     8581 acgctattat cctttcttcc caatgctacc tgcgatttcg ctgaaagaag tagaagtaga
     8641 tggttatcgt ataccagaag gcagttgggt tattttagat ttatatggaa cagaccatga
     8701 tgaacgaaca gttgaagcgc cagattcctt tatgatcaaa cggtatgtag ggaaagcaaa
     8761 agatatttcc tataaagaag aatatgagat gattgcacaa ggcgggggaa attttcgaca
     8821 aatgcatcgc tgtgcaggag aatggatcac tcttcatagt ctacgtgtgt tttccgatca
     8881 gctagtaaat aagtttgagt tctctgtacc tgaacaagat tggacgattc cattcaatca
     8941 gtttccgact tatccgaata gccgagcgct actttacaag aattagatta gcataaactt
     9001 atcgaaaaaa aggaagatca aacagtgctg atcttccttt tttcatatta ctataaattg
     9061 ataaggtcaa ttgatcaaaa tccacgaaat gcttcatcaa tggtttgata atcttcattg
     9121 gataaatgca aatctaaagc ttggacattg tcagatactt gctctggacg cttagctcca
     9181 ggtataacta cagacactcg tggattcttc atgtaccaag ctaaaacaag ttgcgcagga
     9241 gtggcttggt atcttttagc aatggggcga agtacatcga cagcggttaa aatttctctg
     9301 aaacgttctc cttggaaatc tggatttctt tttcgaggat ctccttcacc aaattgtttt
     9361 tcctcaccaa gttcatattt tcctgtcagc aaaccggaag caagaggaaa atagggaaca
     9421 aaactgattt tgtttttttc aagataaggg aataattctt tttcagcctg ccgatggatc
     9481 aaactgtatt tatcttctac tacatcgaca tatccatctg cattcgcttc tttcaattgt
     9541 tctaacgtga agtttgacac accgactgct cggatttttc ctgcttcttt taattcgtgc
     9601 aatgtagcaa ctgattcatt ttttggtgtg ctttcatccg gaaagtggat ataaaaaatg
     9661 tcgatgtaat cagtttgcag acgttttagt gcatcttcca ccgattgttt gagaaactca
     9721 ggagaattat caaatgttcg ccctttattt ggtacatgag cagcttttgt agcgatgacg
     9781 acacgtgagc gatcatattc tttttccttc agtacctcgc caatcagttc ttcggaacgg
     9841 ccgtttccgt aagcataagc ggtatcgatc aaattgatcc cgctattcaa agctgtacga
     9901 actacttgtt ttcctgtctc atcttccaaa ccgtcaaaaa gattatgtcc tccaacggca
     9961 tttgctccta atccaagctg ttctgcgtag acttgtgaat gtccgattct tatttctgcc
    10021 atagctgatt cctccttttt gtctagtcta aaacaaaata ccaaaaagct gaactaaaac
    10081 gtttttgtga ggggaaatag tcagatatct cttgactttg agactggttt ttagtaggtt
    10141 ataagtaata tcgaatgtca atgaaagagc gagtaagttt ttgaaacttt acaagagagg
    10201 atcatcgagg ctgaaaatga tctaaagttt cattagccta aagacactct ggagatggac
    10261 gagtgaaaag ccgtcccgcg tacgagcgtt attcgtaaga ggaaagagaa atctttcaaa
    10321 attaggtggt accgtgtatg aatttcattc acccttgttc atgatgaaca agggtgattt
    10381 ttttatttac atagctaaga aagggagaga aaactatgag tttccaaaga ccaaaaggaa
    10441 caaatgatat tttgccaggc gaatcagaaa aatggcagtt tgtcgaagaa acagcacgtt
    10501 tattattcag agattatcag tataatgaaa tgcgtacacc gatttttgaa cactatgaag
    10561 tgatatcccg aagtgtaggg gatacaacgg atatcgtttc aaaagagatg tatgattttt
    10621 atgacaaagg ggaacgccat gtgacgcttc gaccagaagg tacagcacca atcgttcgtt
    10681 cttttgttga gcataaattg ttcggcccag agtatgcgaa accgttcaaa actttttata
    10741 tgggaccaat gtttcgctac gaacgccctc aaaaaggacg gttacgtcaa ttccatcaaa
    10801 tcggagtaga agcatttgga tcagaaaacc ctgcaactga tgtggagagc atggtgatgg
    10861 cacttgattt cttcaaacag ttggggatca cgcaaatccg gctagtcatc aattcattag
    10921 gtgataaaga aacgcgtcaa gcctatcgtc aagcattgat cgactatctt ttaccttttg
    10981 aatcagagct gagtgaagac tctaaacgcc gccttcatga aaatccatta cgcgtattgg
    11041 acagtaaaga caaacgagat caaaaatttg tggcagaagc tccatctatc ttagattatc
    11101 taagcgagcc tgctcaaaaa cattttgata ccgttgtttc catgctggat gcattggctg
    11161 ttccttatga aatcgacagt aatatggttc gcggactaga ttattacaca catacgattt
    11221 ttgaaatcat gagtgacgca ccaaaaatgg gggcacaagc aacgatttgc gcaggcggac
    11281 gttatgacaa tctagtggaa gaactaggtg gtccttcaac acctggcttc ggatttgcaa
    11341 tgggtatcga gcgattactc ttgactatgg aggcagagga agtagttatc cctgcattta
    11401 atgagttgga tgcttacgtg gtcgcactcg gagacgaaac aaatatcgaa gcattgaaag
    11461 tggtccaagc gattcgaaac tttggttttt cagctgaccg cgacttcatg aaccgtaaag
    11521 caaaagcgca atttaagaca gcagataaag cgaatgccaa attagtattg gtgatcggtg
    11581 aagatgagct tgctagtcaa acgattaatg tcaaagcaat ggcaagccga aaagaaaaaa
    11641 gttttccatt agaagagata tatgaacatt ttgatgaagt ctatgatgaa atgactttat
    11701 tcgagtagga ggaaaaaaat aaatggcaca aagaacgatc tattgcggat tagtttccga
    11761 acaacaatta gaacaaacag ttacattaaa aggatgggta caaaaaagac gagaccttgg
    11821 gggagtaatc tttatcgacc tacgtgatcg tgaaggaatc gttcaaatcg tctttaatcc
    11881 tgaaatcaac aaagaagcat gggaaatcgc ggataaatgt cgcagtgaat acgtgatcga
    11941 agtgactggg gaagttagaa gacgcggaga aaatgcagta aatcctaaaa tggccactgg
    12001 tgaattcgaa gtgattgcaa gtgaaatcgt gattttgaat caagcaaaaa ccccgccatt
    12061 tcaaattgaa gatgatcaag caatcagtga cgaaatacgt ttgaaatatc gttatttgga
    12121 tctgcgtcgt ccaaaaatga ctaaaaacat caaattaaga aatgcgacaa caaaagcgat
    12181 tcgtcattat ttagatgata atgactttct cgatatcgaa acaccttacc tagggaaatc
    12241 tacaccagaa ggcgcaagag actacctagt tccttcacgt gtccatgctg gacatttcta
    12301 tgcgttgccg caatctcctc aattgtttaa acaattgctg atgaatgctg gattcgaccg
    12361 ttattatcaa atcgttcgtt gtttccgcga tgaagattta cgtggagacc gtcagcctga
    12421 atttacgcaa gtagatattg aaacaacgtt cttaacagca gaagaaatcc aaacttacac
    12481 agagggattg attgcgaagg taatgaaaga agttcgcggt atcgaagtca cattgccatt
    12541 tcctcgtatg acctatgatg aagcaatggc tcgctacggc agcgacaaac cggatacacg
    12601 ttttgatatg gaattgatcg atctaagcga tacagtcaaa gaagtagaat tcaaagtctt
    12661 ccaaatggct cttgaaaacg gcggtgtcgt gaaagcattg aatgctaaag gcgctgcaga
    12721 tcgctactca cgaaaagaca tggatcagtt aggtcaatat gtaggacaat tcggagctaa
    12781 ggggcttgca tggctgaaag tagaagaaga cggattgaaa ggtccaattg ctaaattcat
    12841 gggagaagca acagaagcaa tcattaaagc aacagatgca aaaccaggtg acttgctgat
    12901 gtttggcgca gataaaagtg aaatcgtcgc tgctgcatta ggtgctatcc ggacacgttt
    12961 aggaaaagaa ttaggactaa ttgatgaatc gaaattcaat ttcttatggg taactgattg
    13021 gccacaattt gaattttcag aagaagaagg tcgatatgtc tctgctcacc atccattcac
    13081 gatgccaaaa gaagaagata tcccgttatt ggctgaagac cctgcaaaag tctatgcaga
    13141 agcttatgat atcgtcttga acggttatga attaggcgga ggttctatcc gtattcatac
    13201 tcgtgagctt caagaaaaag tactagaaac tctaggcttt acaaaagaac aaatggaaga
    13261 acaatttggt ttcttgttaa cagctttaga ttacggattc cctccacatg gcggtattgc
    13321 attaggattg gaccgttttg caatgttatt agccggagaa gataatattc gtgaagtcat
    13381 tgcctttcct aaaaacggaa aagcagcaga tgtgatgagt gatgcaccaa gtatggtttc
    13441 gcctttacaa ttgttcgaat tgaatattga tgtaacgaat atagaagaat agccgataaa
    13501 aaggtaaaaa ggatttgtcg aatacgcttc ttttgacaac cttgatttaa ttgaaaaaca
    13561 ctttataatg aagccataac atataaagcg gggcttttcc atgaaaaaaa cattcgaaaa
    13621 ttgggcttat catgattaca cgaccaagct ttcggtatct atcgtttatg cgttgatcgc
    13681 atcagtcgca ttgaatttct tttatcagcc aggacatatt tattcaagtg gtattactgg
    13741 tctagctcag attttgacga ctttgtcaaa atcactgata ggattcgaag taccggtatc
    13801 aacaactttg tatttactga atataccttt gtttgtattg gcgtggttaa aaatcgggaa
    13861 gaagtttaca attttcactt ttatcacagt cactttgacc tccctttgta tccatatttt
    13921 accggaaaca gtcctatcta ccgatccaat catctgtgcg ttgtttgggg gggctgtgat
    13981 gggactaggt attgggctag cgttgagaaa cggcctatca tcgggcggtc tagatatcgt
    14041 gagtatcacg attcgtaaaa aaacaggaaa gtcgattggt tctatttcga tctacttcaa
    14101 tgcattgatc gtttttgttg ccggaattct atttggctgg caatatatgt tttacagtac
    14161 gttgtctatt ttcgtcagtg gaaaagtaac tgatgtcgtt tatactaaac aaaagaagat
    14221 gcaggtaatg atcgtaacga aacatccaga aaaagtagta aatgaaatcc aaaactgtat
    14281 gcgtagaggg atcacgatca tcaatgaagc agaaggtgct tataagcatg ataagcagac
    14341 agtattgatg acgatcgtta cacgatacga actgcctcta cttagagaag caatgaaagc
    14401 ttctgatcca caagcttttg taagtattgc agaaaacgtc cagatacttg gtcgtttcta
    14461 tgaggatggc atttagatcg agtttgacaa aaattcatga aatactgctg tagacagacg
    14521 ttcgtttgtc tgcagttttt ttctaccata gtagaatcag caaagaaaaa ctgctttttt
    14581 atttgaaatt ttcttaaagt tgaaaaaaag catgtttatt tttttaaatt tatgggatga
    14641 tagaaaaaaa tagtagagaa aggacacgat tctacttgct attttcactt aatacccgta
    14701 tattacgaat gaaaaaagtt tatgaaaagt tggaatattg aatggaaaaa caaaaaaggt
    14761 taaaaaacag ccgctacatc actgggtttg atgggctccg ttcattagct gtggtgggtg
    14821 ttatcttcta tcaccttctg ccaactagta tgaaaggcgg ctatttgggc gttccgattt
    14881 ttttcgtcgt atctggttat ttgatcacgg atctgctgcg gcaggaatgg gaacaaaatg
    14941 gaaaaatcaa tatttggcaa ttttatgtca gaagaatgaa gagactctat ccggcacttg
    15001 tttttatgct tgtgacagct tcggcttata tcactttgtt tcagcgaggg ctgttaaaca
    15061 atcttcgagg agttgtggtc agcagcttac tgtatgtcaa caactggtgg caaatcaata
    15121 acggtttatc ttatttcgat cgatttgcta atgagtcacc gtttacgcat atctggtctt
    15181 tagcagtaga aggtcaaaac tatttggttt ggccgatctt gtttgtattg ttgatggtat
    15241 ttgttcgtaa aagaaaatgg atcttttata ctgtgttagg tacatccttc ctctccgcca
    15301 tcttgatgat gatcttgtat acaccaggag gcgatccaac ccgtgtttat tacggtaccg
    15361 acactcgtct gttttctata tggttgggaa gtgcgcttgc gtttgtttgg ccaagtacgc
    15421 gtttaaagaa aaacatcccg aaacaagcga aaaatattct gaatatcgct ggaggaatct
    15481 cgcttttggc actgattctt tgcttcttct ttttagatga tcattacaat ttcatctatt
    15541 acggaggaat gctcctagtg agtctgttat gcgtagtgct agttgcagtg acagcacacc
    15601 ccggagcaag tttgaatcga tggctgacaa atccagtatt tacttggatt ggcaaacgaa
    15661 gctatggcat ttatctgtat cagtttccag ttatgatttt ttatgaagca aaggtgactg
    15721 atattgccga tcatgttttg ttgcatactt tagtggaaat cacattgatt ctaatcatca
    15781 gtgaactttc ttatcgatat gtggagcgtc cgctagcacg ttttgattat ggtcgtacat
    15841 tccaagtgat gaaaggatgg ttcagcaaac cgatcatcag taagagcaaa ccatgggttg
    15901 ttccaggcac attacttgta gcaattgcac tcgttggttt cgttacggca ccgaaaaacg
    15961 ctgtaacagc cgatcaaaaa caactgcagg caaaaatcgc tgaaagtaaa aaattagcag
    16021 aagaaaccag aaacaattca gaaaacacca ctagttcggt tgaccaagcg atccttgata
    16081 agtacgagtt gtccaaggca caagggaata aggcacaaaa attagaacta acggcatttg
    16141 gtgattcagt tatgttggat gctgcaacgg atctacaaga actttatcca aaagtagtgg
    16201 tagatggaga tgtcggacga caattatatg ccagtgtacc gtacattgaa aaattaaagg
    16261 aacaagattt gttgaaagac actgttctga ttggactagg aacgaatggc tcattctcgg
    16321 aagctcagtt tgatagtgtg atggatgcaa tcggtgatcg aaaagtatac tgggtcaatg
    16381 tccgagtacc gacgaagcgc tggcaaaatg acgtgaatgc gatgcttgaa cagatgacga
    16441 aaaaatacga caatatgacg ctgattgatt ggtacagcta cagtaatgac catagtgact
    16501 ggttttatga tgatcaagtc catccaaatc ctgaagggat gacacattac attcacttag
    16561 tcagcgaagc cttgctagga ggaagttcaa gtacgaacag cagtgagaca aacgtgacag
    16621 aatgatagct acaaaaaact tcaggcaaaa acatttgttt ttgcctgaag tttttttata
    16681 tttaccgtgt ttttaacatt catatagatt caggaagcaa aacggttgag tgtgtctgta
    16741 aataacgttc tttttgtttt cgggtgagtt ttttgaatgc agcttctgct tttgttgctt
    16801 cgcttcttga agaaaatact tctgcatgga tcatcttaac aggtcttctt ttttgaagac
    16861 gagtatattt agcaccagta ccattgttat gttcctctaa tcgtctagaa agatcggttg
    16921 tatatccacc ataaaatgtc tgatctttac agaataatac gtaaaaataa tgttcatttg
    16981 ccatagagca tctcacgaat ctctgcagtg tattctccat ccatattgta agtgatcaat
    17041 ggtggagcga ctttgaatcc gtctaatttg ccttctttga ttccttcaac taacaaaata
    17101 tttgcctctt ttccaggttt tgggtaaaca aattggatcc ttttaggtgc aattcgatag
    17161 tcttccatga cctgaatgat ttctaagaac cgatcaggac gatggaccat agtaaagcgt
    17221 ccatttgttt ttagtagctt acttgaaata tcgatcactt cttctaacgt cgtatgaatc
    17281 tcatgacggg cgattgccag gtgtttatta gggttcgttt tatttgttgg cagccctttg
    17341 aaataaggtg gattacagac gactaagtca caagaattat gtcgaacgac agataagctg
    17401 tctttcagat cgatggtgtg gacagtcatt tgttcattta aatggttaag cgcgatacta
    17461 cgtcttgcca tgtctgctag acgaggttga agttcgatag cgtcgatagc cgcctgggtt
    17521 cgtttactta aaaaaagtcc aactgcgcca tttcctgcac aaaggtcaac aatcttccct
    17581 tttttaggta cacgggaaaa gtgagcaagc agcacagcat ctaaagaaaa agaaaacact
    17641 ttggaacttt ggataatctt gatatcttct gcgtataatt ggtcgattcg ttcgtcttcg
    17701 tgaagcatgg attccctcca aaatgaaaaa aatagtcgtc tgtattatac tatttttatt
    17761 aaaaagaacc aaggtggctt gtcaaacctc tattttttat gcatactatg accataagaa
    17821 gggaaaggaa gagactatgt tttatcgatt tatgcgcggg gtagttcgag tactattagc
    17881 cttagttaat ggaaatgccc gctatgagaa taaagaagtt ttaccgcaag atgagaatta
    17941 catactagtt gctccccatc gtacttggtg ggacccatta tatctggcgg tagctgcagc
    18001 acccaaggaa ttcagcttta tggcaaaaga agaactgttc aaaaatccag ttctgcgatt
    18061 tatattaact cgatccaatg cttttccagt taaaagagaa aatccaggac caagcgtcat
    18121 taaaacacct gtaaagattt tgaaatcgac aaatttgagt ttgatcatgt ttcccagtgg
    18181 gacaagacat gctacagaac ttaaaggcgg aatggcattg atttccagaa tgtctaaagc
    18241 gaaaatcgtg ccagctgtat atcaaggtcc gttgacactt ggggatctgt tcaagcgtca
    18301 gagagtgaca gtgcgtttcg gcgaaccaat cgacttatca gatatcaaga aaacggataa
    18361 agaaggtatg gcggagatcg aacgacgaac acaagcagct tttgacaaat tggacaaaga
    18421 agtgaaccct gatttcaaat atgaagtcaa aaaaaaataa aatgataagt ttatgcgtgg
    18481 gcggtctttt gaatctactg tcctgttcag ttcagacttt ttcaatgaaa acgagggaga
    18541 gacgtcaagt ctgccctctt tttgcgttcc aatatgtaaa atagaagaca ggtgtcatgt
    18601 aatggatcaa agtgttataa taattagaag gacatttttt gaggtgattg aatgcgtttt
    18661 ttacatacag ccgattggca tatcggaaaa aaactacaag gttatgactt gattgaagac
    18721 cagcgtcatg tactatcttc tatactagat atcgcgaaag aagaaaaagt agaggcaatc
    18781 gtgattgccg gagacttata tgataggagt gtcccagctg ttgatgccgt agaattatac
    18841 aatgaattga tggtcgattg gaatttaaag gaaaagatcc cgatttttgc tatatcagga
    18901 aatcacgata gcagtacgag actttcagcg ggtacgccat ggttcagtta ttcgaactat
    18961 tttttgcgaa cacagctagc agaagcattt gaaccagtcg agtttaaaaa cgtacaattt
    19021 ttctttcttc cttactttga accaattgca gcgagattat attttgaaga tgacaatatc
    19081 cgaacgatca agcaagcggt agccaaggtg atagataaaa tgcaggagct atttgattca
    19141 gaaaaaaaac aagtattgat tagccatttt tttgtaatgg gaagtgtaaa atctgattcg
    19201 gaaacgaaaa tcgaagtagg cgggttggat tcgatccctg gtgaactact tacaccgttt
    19261 gattatgtag cactaggaca tttgcataat caagctgctt tgagacagga taatgctcgc
    19321 tatagcggtt ccccattgaa attctcttta tctgagatca accaaaaaaa aggcgtatgg
    19381 atcgttgacc tgacaacagg cctggagttg aattttaggg agatcgaacc cctgcatgat
    19441 attgaagaaa taacagcgag tttcaaagaa ttgctggatc ctgcctttta tcagtccgtc
    19501 aatcgggaga attacatcta tatctatctc actgatagaa cgatcatccc gaatatgatg
    19561 aaccagcttc ggcaaatata tcctcgtatt ttggggattg aacgggcaaa cggtcgagca
    19621 gaacaaacaa agcgcaaacg aaaagtacta gaaatgaaag atcctcaaaa attagcagaa
    19681 gcatttttta gtgaaatgac ggaagagcag ctaactgcaa accaagaaaa atggctcgga
    19741 gaaacgttgc aagaaatcat ggaaatggaa tgatcaagaa tgaaaccgaa aaaattacaa
    19801 cttaaaaatt ttggtccttt tatcaatgag accgtagatt tttcacgttt gacagaagca
    19861 ccgctttttt tgatcagcgg gaagacagga gctggcaaaa cgacgatttt tgatgggatc
    19921 accttcgcac tttttggtga aacttccgga agacttcgct ctggaaaaga gatgcgttct
    19981 ttgtttgcta caccagacga agaaacaagc gtgacttttt cttttgaaca tcagcagatg
    20041 agctatgaaa tcgaacgaaa accagaacaa gttttagcaa aacgaaaagg aaacggaaca
    20101 aaaaaacagg cagccaaagt aacgttgacc atttttgatg ggaaaggaaa agaaataaaa
    20161 caaatcacaa aacgtacaga agcagatcag ttgatcagag aactgatgaa tctcgatgcg
    20221 aaacagtttt ctcaaatcgt tttactaccc caaggagaat tccgtaattt tttggtttca
    20281 tcaagcagtg ataaagagac tgttttacgt catttattcg gtactcaatt tttccaacag
    20341 ttcaatgagc gattaaaaga aaaagcaaaa caacagcaac aaaaattgga tcatttagag
    20401 caagagctgc atcttctgca aaaaaggttt gttttagtca aagaagagga accagcagca
    20461 gggtttgaag ccgttttagc ccaatggaca aaccatcagg caatactcga aaaacaaatc
    20521 acggaagaaa aagaagctct aacggaactt caggaaaaac aaaagcaact agaaaattcc
    20581 tactatcgtt ttgaaagctt gaaaaaaagt tatgaagaaa aaacgatatt aataaacaag
    20641 caaaaggaat tagctgctca aaaagacgaa atagaggaaa agaaacgatg gattgggtat
    20701 tatgaattta cgcaacgatt agtcgaacca atccagcgcc tacaagaaat aaaagttgaa
    20761 aaaatagaac tgtcacaaaa aagtagtgag caaacaaaga aactagcgat gactacgcag
    20821 aattatgaag agtggcaaaa aaatgaagca aaccgcgtac gccgacaaac aaaaatcaaa
    20881 gaagctgctc aaaaactgca aaacaaacag atgatggtgc caattgtcga atccttacgt
    20941 agtaaaaaac aagacgcaga acaattaaca atttcattgg ataaagaaga attgaatgcc
    21001 gagaagctgt atcaaaatct cgctatctat caggaaagaa ctcgaattct tcaagaaaat
    21061 ttactggatc aggaaaaact gcaagaagat cgtcttgctt ttggacagtt aaaatatcta
    21121 gatgaaaagc agcaagaaat agaaaaacaa aaagcaaaag acactgggtc gctcgcacag
    21181 ataagagaag aactcaaaaa agcaactgaa gaaataacaa aaaatagtca gctattgaaa
    21241 gaacagacag aacaagcaga acagctgaaa agccaatggg caaagcagca aattgctcgt
    21301 cttcagcttt ttttgcgtcc aggggagcca tgtcttgttt gtggatcaac ggaacacccc
    21361 atacaacaat taaaacacga acagccaaat aaggaagata tcttaaaagt agaacaacaa
    21421 ctggcagaag ctgagtctgc tgtagaaaga actcagcaaa tcattgccaa aagtgaagca
    21481 catgaaaata atcttcaaca acaattatca gatttagaac aaaaaatcat ggcaacgaat
    21541 atacaattgg ctgaacaaga agaacacttg caacaggctt tgaaaaaggc atttcctgag
    21601 ataacagaac aaaaaacaaa aacaactctt gtagtaatcg aacaatcttt agatagacga
    21661 aaaagcaaga tggccaatgc aagaatagag ctggaagaaa taagacaaca agcagaaaag
    21721 ctcgaacaac aattagctgt ctacaaacag cagcagacag aaaaaaataa tcagctccag
    21781 caactgcaag gagaaatcaa aagtttagaa caacaattgc aagatacgga tagtaactgt
    21841 ttagaagagg agatacgacg acttgaaaaa cagttagaag aagactcgaa tcaactagct
    21901 gttgagcaga aagcaggagt gcaattgttg caagatcttc atacgcttcg gacacagctt
    21961 gatcttcaac atgaacaaat agaaaaaata gatgaaaaaa tagaaaagga acaagaaaga
    22021 atagctaaaa agttagaaag ccagtcgtat ttttcaacaa ttgaagaaat ctgtgcattt
    22081 gcacaacagc aagcacaata taaagaaaac caacgtacag ttacttctta tcatgaagag
    22141 caattagtga cagctgaccg gctgcgtcaa ttagaaaata tccagctaac tgagacattc
    22201 ccagatacag aagagatcag ccaacagtta gaagaaataa gacgtatgat cgaaataaag
    22261 caagaaaaga tatacggttt acaagaacaa aaaattagta atcaaaaaat ctacgatgaa
    22321 tgtctttcga tttatcacac aagccagacg aaattagatg agcttacgca attacagcag
    22381 ctttcgcaaa cattcaacgg agaaaatcct aagaagacta gtttggaacg gtatgtgctt
    22441 caggtttatc ttcaagaagt gttacaagta gcaaatgatc atctccaacg tttaacgaaa
    22501 aatcgttatc aatttgagct agcggatacc attggcagtt atcgagggaa aaccggatta
    22561 gaaatcaata tctatgatga tcaggcaggt atgtctcgaa gtgcgcatac cctgtctggc
    22621 ggtgagagtt ttattgcagc actttcttta gcattagctc ttgccgatgt gatccagaca
    22681 caagctggtg gtgtagcaat cgatgcattg tttattgatg aaggatttgg gtcattagat
    22741 gaagaagctt tggaaatggc aatggaagct ttagaaacga tcgaaagtga aggaagaatg
    22801 atcggcatca ttagtcatgt aagagaattg aaagaacgag tattacaaca aataagaatt
    22861 gatacagaag gtagcggaca aagcaaaata cgctatcaac ttggataaaa agggagattg
    22921 gtaactatga aatggagtat tccagaaaaa gtcattgcac gtggacgtca atacttgcaa
    22981 gaaggccgag tgttatcggt tgtccctgac caagaagaaa acgtgtggca tgcagaagtc
    23041 ttgggcagtg aattgtatat ggttgatttg gatggtacag caaaagagat agattactgc
    23101 caatgtccct attgggagga acataaatat tgtaagcata cagttgctgt ggaactttat
    23161 ttacggtcga aaaatctatc tcgtatcatg aaaaaagacc agccaatgct agaaccggta
    23221 aaagcaacaa gtgagggaga aatcattgca aaaggttttt ctcgtctaaa aacacaaaac
    23281 aatcctcaag cggtgcaacc tttgattatt gaataccaag tagaaacgat tgagactaat
    23341 cagtatcacc cagagctttc tattttggca ctttacttgc gtatcggttc attggcatca
    23401 ccgaaaaaaa catatatcgt aaaaaacatc tatgattttt tacaagctta cacagaaaaa
    23461 gaaacttaca ctgttaataa acagtaccag tttcgtctag ataaacgggc gtttcaaaaa
    23521 gaagatcaaa aaatactcac gcatcttgca ggaagtgctc agacgcatca attattaggt
    23581 acgaacggct tacaagtcaa aggaaaacta gataaaagat atctgcttct cccagtagaa
    23641 caagcccgct tcctgttaga gcagatgaat cagactacac gtttgcatct gcagataaat
    23701 gaacaaaacg atcaaggaat acaattttcc gatcaaaaca aacctttatc attcaaagtg
    23761 gaaaaatttg gagaagatta tttgctgcaa gcattgaatg attttgattt tttctttatg
    23821 cattatcagt ggggagttat ccaagggaca ttttattctc tgacgaaata tcaacaaacg
    23881 gtttatcaaa cttggaaaca attgattcgc cgattggaga aaccagaagt agtgttcaag
    23941 aaaaaagaat taccgtcttt gtttaaagag attatccctc tgctgtcaga gatcggcgac
    24001 gtagctattg cccctgcagt agaagaggaa gtagcagata tccccgtcga atttattttc
    24061 tttttccgaa aagcaaaagg aaaaatccaa gctcgagtcg atttcgtcta tggtgaagtc
    24121 atctattcga ctgacccgaa gcatgaggtc tcgacagaac aggcttttca ggtgttacgt
    24181 gacacagtag aagagaaacg agtacttgat ctattcagaa tgtatcgcta ccagaaaaat
    24241 gaaactggtt atgaacgtcc attgccaagc ggggaagaac tatatgcctt tttccgcaca
    24301 gaactatctg tcttccgtca gtttggtgaa gtcagacttg gaagaaaatt gcgagagttg
    24361 tatttagatg cgcataaaca tcaaccgaaa atcgaagtaa ccgagaccgg ttcatggctc
    24421 gatattcgat tcgatgtgac agggatagag gagcaagaaa tcgatcatgt cctgcaaagc
    24481 ttgctgagaa atgatgcttt ttatacatta gaaaatggtc aggttctttc ttttgattca
    24541 gaagaatttc aacaaacaag ccaagtgttg caacaattac gggaatcgat acgtacagaa
    24601 gaaggaacga tccatgtacc taaaaatcaa ggattgatca ttcaaaatca attggaaaaa
    24661 agcaatgcta cttttagtga atcgttccaa acgatggtac aagatctcat tcatccagaa
    24721 agataccagg cacagcttcc caaaggattg aatgcaacga tgagagatta tcaaaagcaa
    24781 ggttttcgat ggttgaagat gttaggtcat tatcagtttg gcggtatttt agccgatgaa
    24841 atgggacttg ggaaaacact acagacaatt gcctttttac tttcggaaaa agaagaacgg
    24901 aaaagcttct ctgctttgat cgtagcgcct gcgagcttaa tttacaactg gcaggcagag
    24961 gtgagaaaat tcgccccttc tttaagtatc caagtaatca atgggaataa aaaagaacgt
    25021 gaagaattac tggctaaaga cacagatatc cgagtgactt cttatgccag cttaagacag
    25081 gatctagcta attatcagtc gcaaaaaatt gattatttga ttcttgatga ggcacaaatg
    25141 gtcaaaaata gcagtacgaa aactgcacaa gcattgagag aattagctgt acctcagcgg
    25201 tttgcactga gtgggacacc aatcgagaat aatttagaag aattgtggtc attgtttgca
    25261 acgattatgc caggattttt cccgactaaa acaaaattca gggaacttgc cgcagaagaa
    25321 attgcgcaaa tgattcgacc gtttatttta cggcgagata aacaaactgt gttgaaagac
    25381 ttgccggaaa aaacagagat gaatctatat tctgcactta cagaagaaca aaaaactgtt
    25441 tatttagctt acttgcgtca aatgcgtgaa gaaatcacat caatggattc tgaagcattc
    25501 aagaaaaacc ggatcgggat acttgctggt ctgacaagac tgcgacaaat ttgctgcgat
    25561 cctcgtctgt ttattgaaga ttaccaaggc ggttccggca agctcgaaca agtaaaagat
    25621 ctactggtag ctgccaaaga gaacaaacga agagttttgc tgttttctca atttacaggg
    25681 atgctgacta tcttgcaaga agagttagcc gagctaggga tctcgacatt ttatttaaga
    25741 ggcagtacga aaccacaaga tcggttaagt atggtagacg catttaatgc tggcgaaaaa
    25801 gatgtttttc tgatttcctt aaaggctggg ggaactggat tgaatctcac gggagcagat
    25861 acggtcatct tgtatgacct ttggtggaat cctgccattg aagaacaagc tgctggacgt
    25921 gcacatcgga tcggacaaaa aaatgtggta gaagtgtggc gtatgatctc tgaaggaacg
    25981 attgaggaac gcatggattc actccagcaa gaaaaacgcg aactgttcca aaaagtgatc
    26041 caaggtaatg aagaacagtt gacgaaactg actgaagaag acatccgcat gattttaagt
    26101 gtaggagaaa tcgaggagta agagactcga aaaagcgttc agtaatgggt aagaaagcct
    26161 ttctaaagaa gcgattgttt atttgattta aaaaatagca aggaggctgg gattccattt
    26221 tatcccagct tccttgctat cattttttta tttctatttt taaaggttat acaaagattg
    26281 tcttcattgt ctctaacagc tgatcaaaag aagaaatctc gatatcgcaa attggagtct
    26341 gttcaatttt gcgttctcga tggttgaacc aaagcgcttg ccagttagca ctttttgcac
    26401 ctagaacatc attgtcgtaa ttatcgccaa cgtataaggt ctcttctgga agcatatgaa
    26461 attctttctc agccagctga aaaatctctt tttctggttt ttggaaacct gttgcttgag
    26521 aaatgagcat attgcgggaa ggaatccaat tgttcaattg aagttgcttc agtttttttg
    26581 tctgatggtc agtcggccca tttgtgataa ttcctaatgg gacattcttt tccttcaaga
    26641 aatcgagggt ttttttgacc tcctcatgca agcagatatt atcaagttct tcctcgtaaa
    26701 ttttttgaaa taagaggcca tcttcttccg taatatgagg gtagtctaaa tcttttagtg
    26761 attggctgat ccgatgtgct cgcatatatt ctagtgtcca atctcctgcc attacttttg
    26821 ggaagttttc atcactatga tggcggaacc ggatatatag tggatgcata tctgcatctg
    26881 aaacaagcgg aacgactctt ttgaccgcgt ttcgaaatgg ctgctgctga tcatacatcg
    26941 tatcatcaac atcaaacaca actgatttaa tcaaaatttt acacgccttt cggttttcat
    27001 tgattttttc acaaatcctt gactattgta acagaaaaaa ttgtctaaaa aagctgataa
    27061 ataaaggttt ttcttactat atttctattt ccttgacaga ataaggtttt tatatttcag
    27121 tttttttcac taaaaatcgt gaaacacgtc aataagtttg atgaacaaat taatcattat
    27181 ggtagaagtc gattgattta ttttaatgca atctctgtgg aattttcgaa agatttattt
    27241 ttttaata
//
LOCUS       DAYYGO010000024        27047 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000024 DAYYGO010000000
VERSION     DAYYGO010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27047
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.024"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            205..1209
                     /locus_tag="ACHA5T_000882"
     CDS             205..1209
                     /locus_tag="ACHA5T_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8886478.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            1210..2154
                     /locus_tag="ACHA5T_000883"
     CDS             1210..2154
                     /locus_tag="ACHA5T_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8886479.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            2154..3116
                     /gene="opp4B"
                     /locus_tag="ACHA5T_000884"
     CDS             2154..3116
                     /gene="opp4B"
                     /locus_tag="ACHA5T_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFM8886480.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            3143..4057
                     /locus_tag="ACHA5T_000885"
     CDS             3143..4057
                     /locus_tag="ACHA5T_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8886481.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            4083..5864
                     /locus_tag="ACHA5T_000886"
     CDS             4083..5864
                     /locus_tag="ACHA5T_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8886482.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            6212..6898
                     /gene="rnc"
                     /locus_tag="ACHA5T_000887"
     CDS             6212..6898
                     /gene="rnc"
                     /locus_tag="ACHA5T_000887"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFM8886483.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            6918..10499
                     /gene="smc"
                     /locus_tag="ACHA5T_000888"
     CDS             6918..10499
                     /gene="smc"
                     /locus_tag="ACHA5T_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFM8886484.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            10508..11317
                     /locus_tag="ACHA5T_000889"
     CDS             10508..11317
                     /locus_tag="ACHA5T_000889"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8886485.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            11328..12326
                     /gene="ftsY"
                     /locus_tag="ACHA5T_000890"
     CDS             11328..12326
                     /gene="ftsY"
                     /locus_tag="ACHA5T_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFM8886486.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            12513..14780
                     /gene="gshAB"
                     /locus_tag="ACHA5T_000891"
     CDS             12513..14780
                     /gene="gshAB"
                     /locus_tag="ACHA5T_000891"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFM8886487.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKKYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(14869..15321)
                     /locus_tag="ACHA5T_000892"
     CDS             complement(14869..15321)
                     /locus_tag="ACHA5T_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFM8886488.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            15471..16421
                     /locus_tag="ACHA5T_000893"
     CDS             15471..16421
                     /locus_tag="ACHA5T_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM8886489.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            16414..17373
                     /locus_tag="ACHA5T_000894"
     CDS             16414..17373
                     /locus_tag="ACHA5T_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM8886490.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            17370..18125
                     /locus_tag="ACHA5T_000895"
     CDS             17370..18125
                     /locus_tag="ACHA5T_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8886491.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            18148..19101
                     /locus_tag="ACHA5T_000896"
     CDS             18148..19101
                     /locus_tag="ACHA5T_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8886492.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(19745..20041)
                     /locus_tag="ACHA5T_000897"
     CDS             complement(19745..20041)
                     /locus_tag="ACHA5T_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFM8886493.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            20396..20752
                     /locus_tag="ACHA5T_000898"
     CDS             20396..20752
                     /locus_tag="ACHA5T_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM8886494.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            20762..21661
                     /locus_tag="ACHA5T_000899"
     CDS             20762..21661
                     /locus_tag="ACHA5T_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8886495.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            22181..22909
                     /locus_tag="ACHA5T_000900"
     CDS             22181..22909
                     /locus_tag="ACHA5T_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM8886496.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
     gene            23054..25081
                     /locus_tag="ACHA5T_000901"
     CDS             23054..25081
                     /locus_tag="ACHA5T_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFM8886497.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            25189..26286
                     /locus_tag="ACHA5T_000902"
     CDS             25189..26286
                     /locus_tag="ACHA5T_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFM8886498.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            26593..>27047
                     /locus_tag="ACHA5T_000903"
     CDS             26593..>27047
                     /locus_tag="ACHA5T_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8886499.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKLTRV"
BASE COUNT         9072 a         4678 c         5704 g         7593 t
ORIGIN      
        1 ctttttgaca cgtaaaagtc atttttctga taattctggc gataaacaga aattatgttg
       61 caaagtaaag cgattttcta tataattatt aataattcat tacacaaaga taagaataac
      121 ctttgttata aaacgcttta ttctgtgtaa tgtccttttt tattaagaaa aacttccatt
      181 taggaagcag agaggggtat tcgattgtcc gataaccaat tattagaagt tcagaaccta
      241 catacaggat tccgactcaa agatgactat tacgatgcag tagatgacgt ttcatttact
      301 ttagacaaaa acgagatttt agcaatcgtt ggggaatctg ggtgcgggaa aagtaccttg
      361 gctactacga ttatgggatt acttgatcct aataacacaa aaattacagg tgagatttta
      421 tataacgatc tgaatttaat cgacctgaac gaaacgctct ataataaaat ccgcggaaat
      481 gatatcggaa tgattttcca agatccgttg tctgcattaa atcctctcat gagaatcgaa
      541 gaccagatca aagaaggact ttcctatcac acaaagatga atgcagaaca gcgtcaagct
      601 cgtgcactag aattgctaga acaagtaggg attcctaatc ctgcgagagt tggacgccaa
      661 tatccccatg aactatcagg cggtatgaga caacgtgtca tcatcgcaat cgcaatcgcc
      721 tgcaaacctc caatcgtcat tgcagatgaa ccgacgacag ccctagatgt aaccatccaa
      781 gcacagattc ttgatttact gaaagacctt caggaagaaa caaaaacagg gatcatcttg
      841 atcactcatg acttaggagt cgtagcagaa atggctgatc gagtggcagt gatgtatggc
      901 ggacagttcg ttgaagtagc aggtgtaaaa gaactgtttg atcatcctaa gcatccttat
      961 acacgctctt tactgaactc tataccacaa gaaggaaatc atgatgcaga actccatgtg
     1021 atcgaaggtg tcgttccttc tctgaaaaac atgcctcgta aaggttgtcg gtttgcacca
     1081 cgtattccat ggataccagc aagcgatcat gaagaagagc ctatccttca tgaagtcgca
     1141 ccgaaccact tggtacgttg ctcctgctat aagcatttcc actttagaga cgaaaaagga
     1201 gaggtgtaga tgacagaatt aatcacaatc aaagacctga aagttcatta ccctattcgc
     1261 agtggattct tcaatcgagt aacggatcat gtatatgctg tagatggtgt ggattttatt
     1321 atcgaaaaag gaaagacata tggtttagtg ggagagtctg gctctggaaa atctactacg
     1381 ggaaaagccg ttgtgggatt agaaaaggta acagccggac aaatcatgta tgaaggaaaa
     1441 gatgtaacga agcgaagcaa ccgcaaaaaa atgggttata acaaggatgt gcagatgatc
     1501 ttccaagatt caatgtccag tttgaatcct aaaaagcgag tgttggatat cattgctgaa
     1561 cccatccgaa attttgaacg gttaagcgat caagaagaaa agaaaaaggt aaaaggactg
     1621 ctagatatcg ttgggatgcc ggaagatgcg ctatataaat atcctcatga attttcaggc
     1681 ggacaacgtc aacgtctagg agtagcccgt gctgttgcta ccaatccaaa actgatcgta
     1741 gccgatgaac ctgtatctgc tctggatcta tctgttcaag cgcaagtatt gaatttcatg
     1801 aaacgaatcc agcaagaata cggcctcagc tatttattca tttcacatga tttaggtgta
     1861 gtaaaacaca tgtgcgacaa tatcgcaatt atgtacaaag gtcgatttgt agaaatcggt
     1921 acaagagaag acatttataa tgacccgcga catatctata cgaaacgtct gctttcagct
     1981 attccgcaaa tcgatgtaga aaatagggaa agccacaaag aaaatcgacg aagagtagaa
     2041 caagagtata tccaaaacca aaaagaatac tatgatgcga ctggtcgagt ctacgatttg
     2101 cgtacgatca caactacaca caaagtggcg ttgaaagatg gaggtgcaag ctaatgtgga
     2161 aaacgattct gcgacgtgtt ttattgatga tcccgcaagt tattatttta agtgttctga
     2221 tcttcttatt agcccaagcg atgccggggg atccatttac cggtttgatc aatccaaatc
     2281 aggatccggc agtgatcgaa cagatgcgac aagcagctgg cttgaatgac ccatggtatg
     2341 tccagtactt ccgttggatc ggaaatgctt tgcaaggcga ttttggtcaa agtttcttat
     2401 acaaacgctc tgttgctgac ttgatcggcg aacggatcat caacacttta tatctatcga
     2461 tcttgacagt tgtccttact tatttgattg ctgtgcctct ggggatgctt gctggccggt
     2521 atcaaaattc aatcttggat aagattgtcg taatctacaa cttctttagt tttgctgttc
     2581 ctttatttat cttcggcttg atcatgctat ttgtttttgg ttatcgttta ggctggttcc
     2641 caacaagtgg atcacaaaca ctaggtttgc aaggtggatt tatcactcaa tggatagacc
     2701 gtttgaaata tattctgtta ccatcaatta cccaagcatt tttggcgact gccgtcacaa
     2761 tccaatatct gcgaagtgaa gtaatcgatt cgaaatcgct cgattttgtc agaacagccc
     2821 gttccaaagg ggtaccgacg aataaagtat tcagtcgaca tattttccga aatgcagcgt
     2881 tgccgatggc ttctcaaatg gggtatgaaa tcacttcgtt gattgctggt tctgtagtta
     2941 tcgaaaagat ttttggttat ccgggagtcg gaaaactatt tattgattcc atcggccaac
     3001 gggactatac cgtgatcaca tccttagtat tgattttagg aatcgcgacg ctagtgggga
     3061 cattactttc agatatcatc atgagtatcg ttgacccgcg tattcggatt caataaaacc
     3121 aatcagaaag gagaatggat cgatgagtga aaaaaacaat acagcagttc aagaaagtat
     3181 tcctcctatg gggatacgaa tgattgctcg agaatttaaa aaagataaaa ttgctatttt
     3241 ttcattaggt ttgctagtgg ttttattatt agtgatcttt atcggtgcga tgctgaccga
     3301 tcaagacaaa gtgatgtatg ttagtatctt cgacaaatac gctgcaccag gaacagtttc
     3361 gacacaagga caaaagttca ttctgggagc agatgaaggc ggacgtgatg tattaggaca
     3421 attgatcata ggtgctagaa actctgttac gatcggattt gcgatcacga tccttacttc
     3481 gatcatcggg gttggattag ggatcatctc tggctactac ggagggatca ttgacaacat
     3541 cttgatgcgg atcgttgatt ttatcatgat tctaccgatc atgctgatca ttatcgtctt
     3601 cgtatcgatt atttccagat acaatgtctg gtcctttata ttcatcatga gtgcatttta
     3661 ttgggtagct aaagcccgat tattccgaag caagacatta tctgaagcaa gaagagatta
     3721 tgtcagtgcc tcaaaaacaa tgggaaccaa tgattttaaa atcatgttcc gcgaaatcat
     3781 gccgaatcta agttcattaa tcattactaa tttgacgatg aactttgcgg caaacatcgg
     3841 tatcgagaca actttgactt tcttaggttt cggcttgccg gcaaatgtac ctagtttagg
     3901 tacattgatc ggttatgcaa gtaacggaga agtcttagtg aatcggcaat ggatctggtt
     3961 acccgcatca atccttattt tagttttgat gttgagtatc aattatgttg gacaagcatt
     4021 caagcgttct gcagatgcgc gccaacggtt aggctagtat agaagcgaag ggggaaatag
     4081 gaatgaagaa aacgattttt ggatttgcag cgctgatgtc tgctgtcgtt cttgcagcat
     4141 gcgggggcgg aggtacagat acctctggaa acaactctgg aggcaacggc aataacaaag
     4201 aggaattaac ttttccaatt gcaacaaaaa ataatgatga tgcaatcgaa gacggcgaat
     4261 tagaagctgc ggtcgcaacg gacagtcagt ttaaaggatt gttccagtgg gagttttacc
     4321 aagattcata tgatgcagct tttatggctc cttctcatga accgctattt gcaaacgatg
     4381 acgatttcag aatcacagac gacggagctg caacgcttga tctagatgaa gatacgaaaa
     4441 aagcgacaat caaagtaaaa gacaacgtca aatggtcaga tggtgaagat gtaacggcgg
     4501 acgatgtgat tttcccttac gaaatcattg ggaataaaga ttacactggt atccgttatg
     4561 acgacacttt ccgtaacatc gtaggtatgg aagaatatca tgatggaagt gccgacacaa
     4621 tctccggaat caaaaaaata gacgacaaaa cagttgaaat cgaatataaa gaagtaaacc
     4681 caagcatgct tcaatcgggt ggtggtatct ggtcatatgc agcaccaaaa catacgttag
     4741 aaggtgtagc aatcaaagat atggaatcaa gcgatcaagt acgtaaaaat ccagtgactt
     4801 ttggccctta ttacatgagt aatatcgttg caggagaatc agtagaatac ttgccaaacg
     4861 agcactactg gaatggtacg cctaaattga aaaagatcac gatgaaatct gtaccgatgg
     4921 caagtgcaac tgaagcattg aactctaaat tatatgatat gattttccaa atgccaacag
     4981 atacttacga tacctataaa gaaattgctg gctatacaaa cttaggccgt gaacaaactt
     5041 cttatactta tctaggattc aagttaggta aatggaatgc agatacaaag agtgtggaat
     5101 acaatccaga ttcaaaaatg gctgataaat cattacgtca agcaatgggc tatgcattag
     5161 ataataatgc agtaggtgaa cgtttctatg caggtttgag aagcaatgcg acttcattga
     5221 ttcctccagt attccagtca taccatgatg atgagatgaa aggattcaca caagatctag
     5281 acaaagcgaa aaaactatta gatgatgctg gctacaaaga tgtggatgac gatggtttaa
     5341 gagaagacaa agatggaaat aaactaacaa tcaactttgc ttcgatgtcc ggtggggaaa
     5401 cagcgcaacc attggcagat tactatatcc aacaatggaa agaaattggt ttggatgtcc
     5461 aatacacaac aggtcgtctg atcgaattca attctttcta tgataaatta gaaaacgatg
     5521 atcctgaaat tgatgtttac caagctgctt ggaatacagg aactgatcca aatccaagtg
     5581 gactatgggg accaaatgct gcgttcaact acactcgtta tgaatcagaa gaaaatacaa
     5641 aattgatcaa cgatatcagt tcaagcaaag catttgatga agagtaccaa aaagaagcat
     5701 atcaaaaatg gcaacaatat gcatttgaag aagcatttgc gattccaacg ttgttccgta
     5761 atgaagtatt gccagtcaac gaccgtgtca aagactggga ttggtcttat gatgtaccaa
     5821 acccatgggc aaatgtttct gttacagcag attcacgttc ataatcacta aaaatgaccg
     5881 aaaaagggcg atgtcctttt tcggtttttt gatatttttc tgtttatttc tctaatattc
     5941 aggtcaaggc gctgatttca tgacttctcc aataaacggt gttcaaagtc cagtttctcg
     6001 gtattaagtc aaatttatgc aaaaatatgg aaagccattt tcacaaaatt ttttcttcat
     6061 actcgaaact aaacgaattt ttcacaactt ctcgtcaatc actaggatta ttaaagcttt
     6121 acttaccttt tgaccgaacc atttcaaatt tgacaaaaaa ccagtataat aaggaagtat
     6181 cgaaaaaata tccataaaag gaatgtggaa aatggacaac cagctgacaa aagaactaaa
     6241 agaaaactat aacatcgttt ttcacgatct aaatttgctt gaacaagctt ttacccattc
     6301 atcctatgtg aatgagcatc gcaatctgca attatcggat aatgagcgac ttgaattttt
     6361 aggagatgcc gttttagaac tgatggtttc tcagtattta ttccgtttat atcctcattt
     6421 gccagaagga aaattgacaa aaacacgagc tgcgatcgtt cgcgaagaca gcttgtcgaa
     6481 atttgccaaa gaatgtcact ttgatcaata tgttttgttg ggcaagggag aagagaattc
     6541 aggaggacgt acgcgaccag cattgctttg cgacctattc gaagcttttt taggcgcgtt
     6601 atatcttgac cagggattcg acacgaccca tgcattcatc gaaaaagtga tttttcctaa
     6661 agtcaaagca ggtgcttttt cacatgagat ggatcataaa acaaaactac aagaagtctt
     6721 gcaaaaatca ggtgatgtat caatcgaata ccgattgatc aatgaagaag gtccagctca
     6781 tgaacgagta ttttggatcg aagtttatgt agatgatcaa ttgattggca ctggacaagg
     6841 gaaatcgaaa aaattagcag aacaagctgc ggcggaaaat gcattggcag cactgtaaaa
     6901 gtaagtgagg gaaagctttg tatttgaaac gaattgaaat agccggtttt aaatcatttg
     6961 cagacaagac agtcattgat tttgaaaata gcgtcacagc agtggttgga ccaaatggca
     7021 gcggaaaaag caatattaca gaggctatcc gctgggtatt aggagaacaa tctgctaaaa
     7081 gtctgcgagg cggaaaaatg ccggatatca tctttgccgg ttccgatacc cgtaagcagc
     7141 tgaatattgc agaagtcaca gtgattttag acaatagtga tcattatctg ccattggagt
     7201 acaatgagat cagtgtaacc cgccgttatc gacggactgg ggaaagcgag tttttcatca
     7261 ataaacaaag ctgcagatta aaagatatcc aagaattgtt cttagattct ggattaggaa
     7321 aagaatcgtt ctcaatcatt tctcaaggaa aagtagaagc gattttcagt agtaaaccgg
     7381 aagaccgtcg tgggatcttt gaagaagcag ctggtgtctt gaaatacaag cagcggaaaa
     7441 agaaagcaga acagaagctt tttgaaacag aagacaacct cagccgcgtc caagacatca
     7501 tccacgaatt ggaagaacag ttaactcctt tggccgccca gagtgaggca gcaaaggagt
     7561 ttttacggct aaaagaaaca ctgactcaaa ccgatgtctc tttgatggtt gctgaaatca
     7621 agacagcaaa aaaagattgg gacaacaaac aagcacagct tgcgaaattc aatctagaat
     7681 taggcaaact atctgagtcg atccaagaac aagaatccat tttggcgaag cagcgtaaag
     7741 aaaatgcgca agccgatcgt ctgatcgaaa aaaatcagca agtattatta gatctttcgg
     7801 aaaaactgaa gcagacagaa ggacaaaaag atgttttgca ggagcggaca aagcatacac
     7861 aaaaaagctc gcaagaatat caaacttcat tagcagaagc acaaaaaaag gttaaacatt
     7921 ttgaaaagct tcaagaaagc ttgatgaaag cagcagcaga aaaagaaaca gagatccaaa
     7981 aagctgaagc aaatctcata aaaacacagc aagaattaga aaaataccaa aaatcaacga
     8041 aagaattatt ggcagaatta agagatcaat atgttgattt gatgcaagaa caagcagctg
     8101 taggaaacga actgaagtat ctagaacgtc aatatataca agaaacagca aaaagcaagc
     8161 agacgttagc caagcaatcg gaagtagaag catcagtaga ccgattgatc ttacaaaaag
     8221 aagaattgac acaaaagcag gcgcagctaa aatcatcttt aactgaaaca aaagagaagt
     8281 tagaaatgat ccaacaaaac gggaaaaagt tccaagaagc attagcaaaa gaacagccta
     8341 aaatgtatca attaatgaat caagtccagc agctacgtgc tcgtaagaaa agccttcaag
     8401 aaatccaaga aaactatttt ggtttttacc aaggggttcg attggtcttg cagcataaac
     8461 agcagctttc gggtattgtc ggagcagtgg ctgaactgat cgatgttcct gcagatttca
     8521 cgttagctat cgaagcagct ttaggtgggg cagcccagca tgtcatcgtt gaaaatgaaa
     8581 aagatgctcg gcaagccatc acgtatttga aacagcaaag aggaggaaga gcaacattcc
     8641 ttccactgac gacaatcaaa ccaagacaat taccggctca tatattgaca caagcagcag
     8701 ctgtagaagg gtttataggg attgccagtg aacaagtcag ttatcccgat caaatccaaa
     8761 ctgtggttca aaacctgcta ggaacgatcc ttttagccaa agacttaact agtgccaatg
     8821 caatcgcgca aacgatccgc tatcaatatc gagtggtttc cttagaaggt gatgtgatga
     8881 atgccggagg atccatgaca ggtggagcga ataaacgagg aaaccaaggc agtttgtttg
     8941 tacaaaatca agaattgaaa cagttgacta gtgaatttga agaagcagat aaacaactcc
     9001 aagcacaaga aaaaaaagtt caggagctgc aacaagaaac agctcgttta gccgaagaac
     9061 aagaagtttt gcgtacacga ggcgaacaat tacgttttga agaacaagaa gcgacaaatc
     9121 agctacaaaa catcataaat gaacttgaac gttttgaaaa agaaaagcaa atctcaactt
     9181 ttgagacacg tgaactgcag cagtttatcg aagattacga aaagcaaacg aacgaattga
     9241 aagacaaaca aacagatctt gaatcgcaac gacaaaaaat cgacgaagaa atcaaatcgt
     9301 taagtcaaga aagcgaccag atggaagcca gaagagcaca agtacaaagt caaaaagcac
     9361 aagaacaagc agatcttgct gtcttgaaag agcagtttaa tcatctgcag atccagctcc
     9421 gcggtgcccg agtccaaaaa gcagaggcaa ccgaacgtca agaagcgata gaaaagcagt
     9481 tggcgacatt gacagcagac ttttctgatc atgaagtcac agaagaaagt ttggaaaaac
     9541 aaattaacga attatctgct cagcgagaaa cattaaaagc cgagttggca aaagccaaag
     9601 agcagcgaga tagaaggcaa aaggagatcg ataagcttga agcagtcttg gcagagcgta
     9661 atcgtgagca aaaagcacga ctgtcggaac aatccaaatt agaagtccaa aaagaccgag
     9721 ctgaaatgat tttagataat catttgacct atctgcagtc agaatatcag ctgacatttg
     9781 agaaagcttc ccaagactat caagaaacaa ccgatatcga agacagtaga acaaaggttt
     9841 catccttaaa agaacagatt gaaaaactgg gaccagtaaa cttaaattca atcgaacaat
     9901 acgaacaagt cagtgaaaga catacattct tagcaacaca gcgagatgat ctgttagcag
     9961 cgaaaaacca attgtttgaa acgatggatg agatggatga tgaagtgcgg acaagattca
    10021 aagaagtatt tgaagctatc cgtcaagaat tcaaaattgt ctttccaaat atgtttggcg
    10081 gcggtaaagc tgaattagta ctgacggaac caactgattt gctgaaaaca ggtattgaga
    10141 ttgaagtaca acctcctggc aaaaaactgc aaagtctaag tttgctttct ggaggagaac
    10201 gagcacttac agccatcgcg ttgctctttt ccattatccg tgtctgtcct gttccgtttt
    10261 gtatcttaga tgaggtagaa gcagcgttag atgaagccaa cgtgaaacgt tttggtcgtt
    10321 acttgagcga tttccaagat gacacacaat ttatcgtagt cacacaccgc aaaggaacga
    10381 tggtcgcagc agatgtactg tacggagtga cgatgcagga atccggtgta tcaaaaatcg
    10441 tttctgtacg gatggaagat atcaatgaag aggcgaaaat caacgaaacg aaagtgtagg
    10501 aacgaaaatg atcaaattag tagcaatcga tttggacggt acattattaa atgaacaaaa
    10561 gctgatttca gatgagaata aacaagcatt agcacaagca aaacagcaag gggtcaagat
    10621 cgttctatgt acaggacgcc ccttagctgc gatggcgcat tatctgcaag aattaggact
    10681 ggtagatgaa ggtgactaca gcatcacttt caacggcggt ttggtacaaa aaaatgatac
    10741 tggtgaaatc atggaaaaga aagtgatgga agtatcagat atccaacgct tatacaaact
    10801 ggctcagcaa ttggatctgc cactagatgt tttgtctgac aatgtggttt tacagctgcc
    10861 tagtgcccca aggaagcaat ccttgtacaa tgtgttgaat aagctgctgc aattccaacc
    10921 agcggatata agcgatatta cagatgaatt tgtgcttaat aaggcagtaa tcgcttatga
    10981 ccaagaagaa ttagatccta aaatcaaaga aataccagca gaattccatg aaatgtatga
    11041 gatcatcaaa acaagaagta tgcttttgga atttatgcca aaaggtgtta caaaagctta
    11101 tggtatttct ttattggcaa aagatcttgg tttgaaacaa gaagaaatca tggcgatcgg
    11161 tgatgaggaa aatgatttac cgatgatcca atatgctgga ctaggcgtag cgatggagaa
    11221 tgctgtgcct ttcgtcaaag aagcagcaga tttcgtgaca gccagcaatg aagaaaatgg
    11281 cgtagccaaa gtgatccaaa aatttgtatt agagtagggg gagtcaaatg ggattttttg
    11341 ataaaatcaa gcgtgctttt actggcgaag agaaaacaga agaagttgaa gtacaggaga
    11401 agtacgacaa gggtttagaa aagacgcgta aaacatttgg tgaacggatg aatgagttgt
    11461 ttgccaattt tcgaacagtc gatgaagact tttttgaaga attagaagaa acacttatcg
    11521 gtgcagatgt aggttttgaa acagcgatac gaatcaccga agcattacgt caagaagtga
    11581 aactcagaaa tgcgaaaaaa tcggcagaag tccaaaatgc gatcatcgag aaacttgtgg
    11641 atctctatga agcggaagga atcaatgaag taaatgaact aaatatccaa gaagacggac
    11701 taacagttat tttgtttgtc ggtgttaatg gtgtagggaa aacaacaagt attggtaaac
    11761 ttgcccatca gttcaaaaac gaaggtaaaa aagtattgat ggcagcagca gataccttcc
    11821 gcgctggagc aatcgaccag ttagttgtct ggggcgaacg cgctggtgtt gaagttgttc
    11881 gtggcaatgc aggaggcgat ccagcagctg tcgtattcga tgctttggaa agagcaaaga
    11941 cagagcaggc agatgtttta ctagttgata ctgcaggccg actgcaaaat aaagtgaact
    12001 tgatgaacga attagaaaaa ataaaacgag taatccagcg ggaattacca gaagcacccc
    12061 aagaaacact tcttgtatta gatgcaacca ctggtcaaaa cgcgatggtt caagcaaaac
    12121 aattcaaaga aacaacagat gtgaccggtc ttgttttgac aaaattagat ggtacagcaa
    12181 agggcggtat cgtacttgct atccgtaatg aattgcattt accagtgaaa ttggtcggtc
    12241 ttggtgaagg aatcgatgac ttggagccgt ttgatccaaa tgattttgtc gttggattgt
    12301 tcaaaggatt actaaaagaa gaataaccca tgtctaaaaa gcaggagctg tgacaaaagt
    12361 tgcagctcct tgtttgaatg attgttttta acatagctgt gtttttgatg ccttcatttt
    12421 tatacttatt tgtaagcggt tctcaaacaa tttcgttctt tttcttagac tttttgatta
    12481 taattagatt catatgaatt ggaggtcact taatgatgaa ttttaagcaa ttattattgc
    12541 atgtcaatgc gcgccctttt atcgaccaag ctcgatttgg tatagaacgg gaaggacagc
    12601 gggttgatct tgcaggaaat ctagcaaaaa ccgatcatcc agcaatcttt ggcgatcgat
    12661 cctatcaccc ctatatccaa acagatttta gcgaaacaca aacagagatg atcacccctg
    12721 ttaccgattc tattcccgaa ttatttcagt atctcgctgc tgtttatgat gtgactgctc
    12781 gttctatacc gaaagaagag atgatctggc cattaagtat gccacctgcc ttaccggaaa
    12841 aagacgaaga gatcattatt gcaaaattaa aaaatttcga agatgtcttg tatcgacgtt
    12901 atttagcaaa aaaatacggg aagcgaaaac agatggtaag cggtattcat ttcaattttg
    12961 aatttggcga tgagttgcta agaacattgt tcagccatca ggaagaattt caagattttt
    13021 ccgaattcaa aacagaactt tatttgaaaa cagcgagaaa ctttatgcgc tatcgctgga
    13081 tgatcactta tttattcggt gcttcaccaa tgagtgagaa gaactacttt ttagatgaat
    13141 cacatccgca agaacctgtt cgcagtattc gcaacagtgc gctgggctat acgaatcatc
    13201 caaatgtgaa agtttcctat gcttctatga aacagtattt agcagatatc gagcgaatga
    13261 tcgaagaagg gaaactttcg gaggagaaag aattttatac accgcttcgt ttccgaggcg
    13321 gaaagaaagt cgcagattta gcaacaacag gtgttcgtta tattgaattg cggaatatcg
    13381 atttgaatcc ttatgcaaga ttgggtatca atccagagca agttcggttc ttacaattat
    13441 tcctgatgta catgttatgg acagaagaaa aagaagactg tgaccaatgg gtagcagaag
    13501 gtacgactcg aaataacaaa gtagcactag aacaaccatc agatcaaaca gaatttcatc
    13561 aagaaggtag agaaattctt gaaggaatga agcaaatgct ggttgaattg gattggttgg
    13621 attctcttta tttagtagag gaagcattga ctcaaatgga tcatcctgaa caaacgttag
    13681 cagcgaaact ctatcaagaa gcacagctat cgagtcagca agaagttgct gtggcattgg
    13741 gacatcaata ttataaagaa agccatgaac gtccctatca gttggccggt tttcgcgaaa
    13801 tggaactttc tactcagata tttatgttcg atgccatcca aaaaggtgtc caagtcaaag
    13861 tattagatga gtcggatcaa tttttgcgtc tgcaattcca agaccatgta gaatatgtga
    13921 aaaatgcaaa catgacaagt aaggacagct atatcgtccc acttatcatg gaaaacaaaa
    13981 cagtaacgaa aaaagtctta aaagaagctg ggttccgggt accaggaggc gcagaatttt
    14041 catccatgga agaagcggta aaagcttatc cgagatttgc cgagcaggca tttgtgatca
    14101 aacctaaatc aaccaattac gggttaggta tcacgatttt caaggaagga gctagtctgg
    14161 aagattatca agcaggttta gccattgctt ttcgtgaaga cagttcggtt ttagtagaag
    14221 aatttatgcc gggaacggaa tatcgtttct ttgtgatcga cggagaagtc caagccatca
    14281 tgttgcgagt ccctgctaat gttattggtg acagtatccg aacagtgaaa gaacttgttg
    14341 aagaaaaaaa tagtgaccct ttgcgtggaa cgaatcatcg tgcaccttta gaattgatcc
    14401 aattaggtga attagaacaa ttgatgttga aagaacaagg gttgacgatc gaatcagttc
    14461 cccaagccaa tcaaattgtt tatctaaggg agaactcaaa tatcagtaca ggtggtgatt
    14521 cgatcgatat gactgatgaa ttttctgaag cttacaaaaa aatcgccgtt tctgcggtag
    14581 aagcactagg agctaagatt agtggtatcg atttgattat accagataaa gaaatcgatc
    14641 ctacaactga taaaaaagca tatgggatca tcgaagcaaa tttcaatcca gccatgcata
    14701 tgcatgttta tccttttgca ggaaaaggca gaagattgac gatgaatgta ttgaaattgt
    14761 tatatcctga agtattttaa aagaaaaagc ttttgacaga ttgtcctatt tgatgtcaat
    14821 ctgatcaaaa gctttttttg catgtaaaaa tttatgtgtt ttttcgtttt aaaataaacg
    14881 atcccagaag ctttttttct tttttggttt ttctttttca gctggcgaag agttttcttc
    14941 tgggtatttt ttttcgatat caatcacagg ttcatttact gcttcttttg ctgttaagag
    15001 caatccgaat tcctcaggat ttgcagtgac gtaatcgttg actacagtga aacggctatt
    15061 gtttttcgtt gctagctgga tatattggtt ttggacagaa aggggtagag ccccattcaa
    15121 gagtatggaa gcatctggat gttttttgag ttcttttaaa aagttttctt tgttttttgg
    15181 ttggatcatt tcagcaacag tcatactaag atagcagcgt tccctgaatg tccctaaata
    15241 tttatgctgt tcttcagggt tcactaaagg tgtaccataa agtcccttat ccaaatggct
    15301 ttttacatca tcttgtgcca taaaaaatac cctccttctt ctctttgtct ttcttacgat
    15361 tatagcatat tcccaataat acctaaaaca tatgaaaaag cggttgcttt tgttcaacga
    15421 ttcacttaaa ataagaatga aaatcattct caattagaaa ggtggattgg atgcgctacg
    15481 gacgactagt agcattatta gttattttaa gtagtatttc attgttcatc ggagtatcga
    15541 atgtacaatt gtcagaactg ctccagttag atgggcaaag cttacaaatc ttgctagtga
    15601 ctcgtttgcc acgaacgatt tgtttgatcc ttgtgggagc gaccagtagt atttgtgggt
    15661 tgatcatgca acatttgacc caaaataaat ttgtttcacc aacgactgcg ggcacgatgg
    15721 atagtgcgcg tttaggtatt ttagtggcta tgattttcct acctggtgca tccttgattg
    15781 tccgatcctt gacagcattt tgctttgctt ttgcaggaac attgcttttt ttatccctta
    15841 cgcgattgtt cctccagaaa aatcaattga tgcttccgtt gattggagtc atgttaggga
    15901 atatcattgg ttcagttgct actttttttg cttatcaatt ccagttggta caaaatatga
    15961 cttcatggct tcaagggaat tttgcgactg tgatacgagg aaattatgag ttattgtatg
    16021 ctgttgttcc attgttgctc ttggtttggc tatttgctta ccgatttacg atcattggtc
    16081 tgggcgaatc gtttgcagta aatctgggaa tagattacca gaagatgcag tttttaggca
    16141 ttggcttagt atcattggct agtgcagtca tgttgatcat ggtaggaagc attcctttta
    16201 tcggagtgat cattccgaat cttgtctcta gaatatatgg agatcaagtg cacaaaacaa
    16261 ttggcgtaac tgctttagca ggtagtttgt tcttagtatt ttgcgatctt gtcgctcgcg
    16321 tgttgatttt tccttatgaa ataccagtca gtgtgatcgt tggaatcatc ggtggggcta
    16381 tcttccttta tcttctgata aggggacgac gatatgctta aaaaaaagcc atctattttt
    16441 attggtatgc ttgttcttgt tttatttgtt acaggaatgt acttgacgat ccatacatat
    16501 ggaaactggc attttgcatg tgcattgagg agtaagaaaa tttttgcatt tatccttgtc
    16561 tcgctagctg tcagcagtgc cacaatcagc tttcagacaa ttacgcataa ccagtttttg
    16621 actccttcag tattgggact tgagtcgtta tatgtccttg tccaaacggt ccttttcttc
    16681 tttatcggag gagtgacgat gcttcagcag acaggtacag tttttttctt attgaacgtt
    16741 gggctgatgg tcgggctgag tctattgtta tttttctttc ttttgaaaaa aatgagagcg
    16801 aatctttttt tattactgat gtcagggatg attttaggta cgctttttgg tagtgtcagt
    16861 acctttttac aagtcatcat ggatccgaat gaatatgatt tgcttcaagg cagattattt
    16921 gcaagctttg gcaatatcca gagtcagtat cttttgcctg cttctttgat cattatcggc
    16981 tgctggtacc tgttatttcg atatcgacat gtgctagacg ttttccatct cggaaatgaa
    17041 caagcagtaa acctcgggat agacacagat cgttttgaat ttttgatttt atgttgtgtc
    17101 agtcttttga cagggacatc aacggctctt gtgggtccag ttactttttt agggtttatt
    17161 gtcgcaaata tcagttatcg tgtcttttct acttatcgtc atggtcagct atttatcgga
    17221 agctttctgg tagctatgct gtttctgaca ggaggacagc tcgcagtaga gcagattttc
    17281 ggctggaata caacagtgag tgtcgtcgtt gaatttgtcg gaggaattta ttttgtcaca
    17341 aaattgattc gcgaaaggaa gagtatccta tgattgattt acaaaaggtc aataaactgt
    17401 accatggaaa atcagtggtt aaagatgtac agttaacgat tcctgaaaag aagttgactg
    17461 cttttatcgg tccaaatggt gctggaaagt caacggtgtt gtcgatgatc agtcgtttga
    17521 taccaaaaga tacaggtgaa atctatttag accataacga agtaaaggct tggaaatcca
    17581 atgaattagc caaaaaatta tcgatcctac gacaatcgaa tgaaataaat gtccaaatca
    17641 ccgtgaaaga attagtagaa tttggacgat ttccttatac gaaaggaaaa ttgacgacaa
    17701 aagacgagga aatcgttcag gaatcactgg aacaccttgg gttagaagaa atagcggatc
    17761 aatcgattga cactctttca ggcggtcaac tccaacgtgc gtatattgca atggtgctag
    17821 cgcaagacac ggattatatt ttactggatg aacctttgaa caatctagat atgaattttg
    17881 cggttcaaat catgcagatt ttaaaaaatt tagtacaaga aatcgggaaa acaattgtta
    17941 ttgttcttca tgatatcaac tttgctgcaa gttatgcaga cgaaattgtt gcaatgaaag
    18001 atggacagtt atttacgcaa ggaacgaccc aagaaatgat aaaaaaagat gtattggatg
    18061 aattatacga aatggacata cggatttgtg agattgaagg ccgcagattc tgtctttatt
    18121 tctaaaacta acattaggag agaagagatg aaaaaattag tagtaggtat cttgattcta
    18181 gcatcatttg gtttagcagc atgtggaaat agcaacaaca ccagtcaggc agacacaaaa
    18241 aaaagcagca cacaaactga aactacattg acgataacag atagtaatgg agaccaaatc
    18301 gaggtgccaa ataatccaga aaaagtcgtc gtattcgaca atggttcttt agatacgatg
    18361 gatgctcttg gtgttggaga taaggtagtt ggagcagcta caagcagcct tcctgagtat
    18421 ctttcatcct ataaaaaagt agaatcagca ggtggaataa aagaaccaga cttagaaaaa
    18481 attaatcagc ttcagccaga tctaatcatc atttctggcc gacaaagaga ttttcaaaaa
    18541 gatctaagtc aaattgctcc gaccattttc ctttctttag atgcgaaaaa tccatgggaa
    18601 tctttccagc aaaatgtcac tgctttaggg gagatctttg gtaagcaaga agaagctaaa
    18661 actcaattag aggaattatc tagtgcaatc gatcagacga aaaagaaagc agaagctact
    18721 gacaaaaaag cactagtcac acttgtgaat gaagggcaat tatcagccta cggatctggt
    18781 tctagattcg gtttcatcca tgatttattt ggctttgaac aagcagatga tcaaatcgaa
    18841 gcatccactc acggtcaaag tgtttcttat gaatatgtgc tggaaaaaaa tccggatatc
    18901 ttatttgtag tagacagaac aaaagctatt ggtggagacg acagcaaaga tgatatttct
    18961 gccaatgaac tggtagcaca aacgaatgct ggtaaaaatc agcagatcat ctcccttgaa
    19021 ccagatgtct ggtatttaag cgggggcggt ttagaatcaa tgaaactgat gatcgaagat
    19081 gtgaatcaag catttaaata gcataaataa cagaggatta aactctcata ctatgtaaca
    19141 ataatgtttc ttacattctt aaatagcaag agcctatcct caaagaagtt atcaaaaacc
    19201 atccgtaaaa tcgtattgat gattttacgg atggttttta tttatttgag aaatcttttt
    19261 tcaacgtgaa ttcattatct acatcatacg ggttgattct agaatgggga gtggcaaaaa
    19321 atagtgtgat ccattttttc agcatcaaga tcgcctcctt tgatttgaat caaaggtaca
    19381 aagcgaaact tagataaaac ttaaatttag atatttttat tttgtaccga tctcttcttt
    19441 aaacgatgtt ccatctgctg cttctcggca ataagtccac tagactagaa aatatgagaa
    19501 gctattttct gtccagcgtt cttattactc aaaacaaatc gcagttgtca caatctcttc
    19561 taaacgatgt tccatctgct gcttctcggc aataagccca ctagactaga aaatatgaga
    19621 agctattttc tgtccagcgt tcttattact caaagcaaat cgcagttgtc acaatctctt
    19681 ctaaacaatg ttcaatcagc tgaccttcgg tattagctca aagcactgat ttcacaacct
    19741 ctatttaggc atgtgtctca gcaagcggtt gatagttgat gatctgcatt tttcgataaa
    19801 aatcggcggg aagcttactc caccgtttta gtaattgata gaaataactc tcttctgctt
    19861 tgattttata ctcgcttaat atcaatccaa aactattcgt tttttttgaa gctaattttt
    19921 tataaatatc caatgctttg gttttatctt cattgacaaa gagtgttttt acttcaatat
    19981 aagaaccctc gatattttgt gtttcttcga taaaatagat gttttttata tattccttca
    20041 tagtgattcc tccaaagaag tcttttattt gataagttta ctctaccgaa gagttgttaa
    20101 taatccgtga acaaaaagta attctttttc caatagttgt taaaggtttt ttatttttta
    20161 tagtattttt tgttatttaa gaaaggaata aggttaaaca aataacaggt tgctttttgg
    20221 gtctttttcc gttcaagaaa ctgaacgaag aaaagggaaa gttgtctctt ttgtgataag
    20281 agaaatcata ataaacgaat gacttacttt ccaataaatt gagaacagtc agaaccagac
    20341 aaattagttg catctgatga ataaagtgat tattctatga catgaggtga tacagatgaa
    20401 attagaagtg acaaaagaag cacaagaagt attgaagaat aaattagaag cagacgatca
    20461 acttttatta gatatcgaaa acggtgacgg tccatttgca gaaagccaag tcagttgcca
    20521 attggatacg gcatttcgat tgatcattgt caaaaaagat acacaaacag atctaagtct
    20581 ttattctgta gtggctgata caccagttgg accaatcaaa atcaaagaca gtgcaatact
    20641 ttacatggat gatccatcca cacttgcttt agaaccaact tataatagtt tacaattaaa
    20701 aggaccaagc ggcttgagaa aaggcaactt gcaagttgtt cgaaaagact agagggataa
    20761 aatgggaaaa ttggcgatca tcagcgacct acatgtggac atcaataaat tcggcgaatc
    20821 agagctgatg cagctttggc aagttttgca aaagcacaag atcactcggc ttcatctagc
    20881 cggagatacg gctaataaag tagaccgttg cctagctgtt gtcgaatttt tcgtcaataa
    20941 aggattatct acgacatttc atttcggcaa tcacgagttg gcagatgtgc ccaatgaaca
    21001 gatgatggac cattttccag acccacattt tttaaatgaa cggtatattg ctttaaatga
    21061 gcagaccgtt cttttaggga tgaatggctg gtacgactat cagttttccg aattgaaaga
    21121 ttacgaggca atcgtacgaa tgaagcgttt gtattggtat gatcggttca tccagcgtgg
    21181 gaaaacagat ctcgaggtca atgcagaaat gcttgcagct gcaaaaaatt tgctagacca
    21241 gctggaagca aaaaaacttg aggtgatttt atccactcat ttcgttccta aacgggcatt
    21301 tatcatgtat caatctgctc cttatgagcg ttggaacaaa ttaaatgcat ttttaggatc
    21361 agaatcattc ggagaattac ttgaccgtta ttcaaatatc aaacaagtag tttttggcca
    21421 tacgcatcgt cgctttgaag ataaatccat cggtcacacg atttacagct gcagaccttt
    21481 tggttattat tatgaatggc agctgacccg agattttgtc ttgaaagaac agttgctaga
    21541 cagctatgat cctatgaaat tacgtgtcct cttgcgtgcc catcacccag cttttcaggc
    21601 ctatcaaaca caacatttgc aagaggaatt tgaacaagcg atgacaatcg ttccgtattg
    21661 agacagcatt agtaatgtgt aaaagaaaat accttgtaaa acataaggat aagtaagagc
    21721 agtagatcca tttatcgtgg atatgctgct ttttttgcgg aaaataatca gcgaatgttt
    21781 atttgatcgg atgatttgta actcgcagag tactagatgc aagcgtgtat tctttttttg
    21841 aacgataatc acaaaaaaaa atataatata cattttttat ttaattataa ttaatattga
    21901 ttgaagtcta ggcagtcaag tcacattttg atagatagga gggggactta cgaattattt
    21961 taaaggctat gacagctgta tttctagatt gtgtttgcat caagttgcaa taaaaactag
    22021 aaatcaaatc gggctgataa tcatttataa ctaatcgata cataggacca aatataggtc
    22081 tatgttcttt tatttcataa gtatcgtaaa acagaatttt gctatggatt aatccaatcg
    22141 actgatatgt ttagtaaata aagaaagagg aatgtcaatg atgaaaaaag aaatcgtatt
    22201 aacagcagca atgatgttat tcagcatggt agcaagtacg acttttgttt ctgcaactga
    22261 agtataccca aaggaatata acacagaagg aacgattaca tttgaagcag gagatgaggg
    22321 tgtaactcct cctgttgatc cagagaatcc tgatcccaat aaacctgtcg atccatcaga
    22381 tcctccaagc cctggaacag gaggggcatt atcgattgat tatggatcaa aattcaaatt
    22441 tgggactcaa aaaatttcta ctgcggataa aacgtattat gcagctgctg atgtgatgaa
    22501 cgatgggtct agaaaaccga catatgtcca agtgacagat aggagaagta ccttatctgg
    22561 gtggaaatta agtgtctctc aaccagaaca gtttaaaact gccagtgggg atgaactagt
    22621 tggagcacag ctgaaattta cgaaagggca ggctgtttct ttggttgatc caacatatac
    22681 gccgcaaacg gtgaattctg aattaacgtt gacaccggga ggaaacaata cgttagcaat
    22741 taatgctaaa tcaggaacag gtgtggggac ttgggtctat cgatttggtg caaatgaaaa
    22801 tgaaaatcaa gatgcggtac agctttcggt acccggtaaa tctgtcaagt tagcgcaaca
    22861 atattccact aaattagttt ggacattaga agatacacct aacaactagt ttactgaaaa
    22921 gaactgttaa aaataaatac gtagtcatat tgtcttgcga ggtaatcgag caaaaattgt
    22981 caaaggctag gctaaatttg tttggatacg aatagttcat ctaggaattt aacgatcagg
    23041 aggaaaagat atattgaaaa agaggaggat cgtgctcatt ttaagcagtc tcttagtgac
    23101 taatatcttt ccgatcatag ggtactcttt ggaattgaat cagttggagc atggaaatag
    23161 aagagacgaa gaaagcaaac tcacttcgac attcgacagt ttaatagaat ccaaccagac
    23221 acaagaaagc agtgaggatt taagtatttc gagtagtgga gagaccactg acaacgtaga
    23281 agctgagggt tcggacaaac aaggactaga agatgcacca atttctgcgg ttgagctgcc
    23341 tgacgatccc aatattattt ctatcgataa aatttttcaa acacctatcg gtactagcac
    23401 gtccatattg gaaaatggga agttactgca gttgaaccca gcagaaaggt ctcaaagagg
    23461 agctatttgg tcaaataaga agattagttt actctcggat tttacattta aatcttattt
    23521 atacttagga aatcaacgag atagagcggg agatggcatg acctttacat tgactggcga
    23581 tcctagaatg gctagtaatc ccagtcaagt tattggatcg ccaggtatgg gaattggctc
    23641 atattctact cgttctggcc aaccttacat tgcgaatgcg ctctcgattg agtttgatac
    23701 gtataaaaat agtggttctt ctgatcgaat ggatcgagag ataactgctg ataatagagg
    23761 gcatggacat gtcgcttttg tcacgcctaa accaaataat aataattatt ctggagagca
    23821 tactggcgtg acggtagctc ctacatttct ttctaatggc acatggcgct tgttaataat
    23881 taaatgggac gcggagcaaa gaagtcttac ttatgatttg gaaggtgtcg gtcgctcttc
    23941 tcggattata cctgatctca ttaggcagtt tggttctaca gaagtaaatt gggggtttac
    24001 ttcttcaaca ggtacttttt ttcaagaaaa tgctgtcgct gtcacgcgac ttccatcgaa
    24061 tgtcacatca acagctgatg taaaagtcaa cgatagtgat tatggggcca tcgctgaagc
    24121 tggaggaaac gataagataa ctatcagaaa tacattgaag gtcgaggggg attttcttga
    24181 atataagaaa atgcagacta gtatcgacct cccacaagaa ttaactattc ccgagcatat
    24241 cttgatcaat ggaaatcaaa ttgatgccaa agatatcttg ataaccggaa atcgagtcac
    24301 gctaaatctg acagattatt ttattgctgg agatacgtta ataatcgaat tggagacaac
    24361 tttgcaggaa gtaattcctg aaaaagtact cactatgcat tttgaatacc ttgaaaacgg
    24421 gaatttgatt gagcaatcga atgaaataaa cctaaagatc gcaaagctga aggagataga
    24481 tatccatgtt tattataaag ataaagacag tcagattgat attgctgaaa tgaaaacaat
    24541 cactgggaaa ataggagaga agtataaaga aactcctatt gatattgaag ggtatgtttt
    24601 tgactcggat tcagcgaata cagaaggcat cttctccgaa aatacagagg atatctattt
    24661 ttattacaga aaaggcaaac tttatctctc aactgctcct caatatgtca attttggaaa
    24721 acataaaata tccagtagac cattaagtga attcggacag ttttcaggtg ggttgaaaat
    24781 tatagatgaa cgggcttttg gaagttggaa attacaattg aaacaagaga aagttcttac
    24841 aaatggagat tttgagcttc cagaagctct ttcctttgtt acctctgaaa atacaacggt
    24901 tatagggaca tccgctgtta cgattttcga aagtgatcaa aagggtgaaa gcgatttgtc
    24961 ctctctttta gattctacag ctcatcgtgg aatcagaata aatattcctg tagaaaacca
    25021 aagaatcgga acctttgagg ggaaactatc ttggatactt gcagatgtcc caggaaatta
    25081 acaggaagag aagaatataa caaaattatt gagtttcaag aagggcacag gtaccatagg
    25141 ctcttgtgtc ccacagagac aagattttgt tatagtcttc tcttacatat gattaaagga
    25201 gcgattttct tgagaccaaa acaacaatta tttataacac tcctagttat tttaggggca
    25261 ctttttaaca atatatcagt gtcagctagt gcatttaatt tttcagtgac gccgataact
    25321 tcagagaatc aaatcgacaa gagaaaaact tacttcgatc ttcaacttga tccggatcag
    25381 gaagtagaag taaaagcgga attgagaaat gatacagaaa aagaagtgaa aatcgatatt
    25441 tctgtgaata gcgcaaccac caattcaaat gtgatggttg aatacggaaa aaacgagatt
    25501 gaaaaagaca tgagcctgat ctttgatctg gtagattatg tgagctatcc acagatagtg
    25561 acgctaaaac ctaaaagtgt ccaaaccgtt gcttttcatg tcaggatgcc gaatgagcga
    25621 tttgatggtg tattggctgg tgggatcaca ttccaagaaa tccagacgga aaaggaccaa
    25681 acagaaacta aggatcaaag cttatcgatt gagaatgaat acacctatat tattgctttg
    25741 ttgatgcaac agacactgaa cgaagttgct ccgaacctta ctcttcatga agttaaacca
    25801 gatcaaatca atgcaagaaa tgtcatctta gcaaacgttc aaaatgatca aaaaacctat
    25861 atcaatcaag tagttattga aacaaaaatt acaaaaaaag gacattctga agttttatat
    25921 caagaggaaa aagaaggatt gcagattgcg ccgaatacaa atttttcttt tccaactgct
    25981 ttaaatggtc aaccgctaac cccaggtgaa tatcacctta caatgactgt tttaggaaat
    26041 gaaaatgaaa atggaaaatt ttcgagaaaa aaaggaaata ctacgatcaa ctataccaat
    26101 caatgggtat ttgaaaaaga ttttacaata gacggaaaag tagccaagga attgaacacg
    26161 acggatgtta cgattaaaaa agacaataat tggatttata tcttagtagc ttcacttttc
    26221 ttattgttta ttttattatg catacttttg tttgttttga gaaaaaaaga agagaaagaa
    26281 aagtaaggtt aagataacaa atagtaaatt tttattattg taggatggtt gggctctttt
    26341 tataacaata taaaatatac cattttatta ataaaaatgc atactagtaa atgatataat
    26401 tctaataaca ggtttgatcg cgagaaaggc caaataaaac gctctccaaa atgttaaaaa
    26461 aaagacagga cgatgtatat tatacaaaca agaatcgctg acaacagtga ctcggatcca
    26521 ggaaatcagt tttatcccca gctagttggt gaatgcatgg aaagtaatat acgtagaaag
    26581 agctgatgac taatgaatta tgagtttttt ttaagcaaag aagatcacag aagttattca
    26641 ttacttaaat atttagaagc aagctcaaat ttatcagagt caatatcgaa tgttcaagaa
    26701 gagttgtcgc tatcgacatt tctcttgaaa aaaacgatcg ataagctgaa acatgattta
    26761 caaaagttcg atctagagaa aaatttgcga gtaaccgtca gtgaattgga tatatgtctg
    26821 gaaattaatg gaaaatgttc tagcaaagct ctgttatctc gttatataac ggattcttta
    26881 tctatgaaaa tggtattggc tttctttcag gaaaaatata tctcagtaga aaattttgct
    26941 aaacagcatc acgtgagtta ttctgtggct tataaagtat tacaaagact aaaaagaaat
    27001 ctaaaaaaat atcaaatttt ttttgaaaag agaaagttga cgagagt
//
LOCUS       DAYYGO010000025        26774 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000025 DAYYGO010000000
VERSION     DAYYGO010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.025"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(59..187)
                     /locus_tag="ACHA5T_000904"
     CDS             complement(59..187)
                     /locus_tag="ACHA5T_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFM8886500.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="ACHA5T_000905"
     CDS             complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="ACHA5T_000905"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFM8886501.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1674..2729)
                     /locus_tag="ACHA5T_000906"
     CDS             complement(1674..2729)
                     /locus_tag="ACHA5T_000906"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFM8886502.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="ACHA5T_000907"
     CDS             complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="ACHA5T_000907"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFM8886503.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="ACHA5T_000908"
     CDS             complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="ACHA5T_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFM8886504.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="ACHA5T_000909"
     CDS             complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="ACHA5T_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFM8886505.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="ACHA5T_000910"
     CDS             complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="ACHA5T_000910"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFM8886506.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="ACHA5T_000911"
     CDS             complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="ACHA5T_000911"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFM8886507.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10491..12050
                     /locus_tag="ACHA5T_000912"
     CDS             10491..12050
                     /locus_tag="ACHA5T_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFM8886508.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12161..14092)
                     /locus_tag="ACHA5T_000913"
     CDS             complement(12161..14092)
                     /locus_tag="ACHA5T_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFM8886509.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="ACHA5T_000914"
     CDS             complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="ACHA5T_000914"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFM8886510.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15021..15773)
                     /locus_tag="ACHA5T_000915"
     CDS             complement(15021..15773)
                     /locus_tag="ACHA5T_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8886511.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(15932..16141)
                     /locus_tag="ACHA5T_000916"
     CDS             complement(15932..16141)
                     /locus_tag="ACHA5T_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886512.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(16152..17480)
                     /locus_tag="ACHA5T_000917"
     CDS             complement(16152..17480)
                     /locus_tag="ACHA5T_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886513.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            17616..17771
                     /locus_tag="ACHA5T_000918"
     CDS             17616..17771
                     /locus_tag="ACHA5T_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFM8886514.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(17845..18432)
                     /gene="yihA"
                     /locus_tag="ACHA5T_000919"
     CDS             complement(17845..18432)
                     /gene="yihA"
                     /locus_tag="ACHA5T_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFM8886515.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="ACHA5T_000920"
     CDS             complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="ACHA5T_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFM8886516.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(20012..21301)
                     /gene="tig"
                     /locus_tag="ACHA5T_000921"
     CDS             complement(20012..21301)
                     /gene="tig"
                     /locus_tag="ACHA5T_000921"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFM8886517.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(21455..22387)
                     /locus_tag="ACHA5T_000922"
     CDS             complement(21455..22387)
                     /locus_tag="ACHA5T_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886518.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(22544..23203)
                     /locus_tag="ACHA5T_000923"
     CDS             complement(22544..23203)
                     /locus_tag="ACHA5T_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFM8886519.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="ACHA5T_000924"
     CDS             complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="ACHA5T_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFM8886520.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGASGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(24244..24534)
                     /locus_tag="ACHA5T_000925"
     CDS             complement(24244..24534)
                     /locus_tag="ACHA5T_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886521.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            24944..26749
                     /gene="glmS"
                     /locus_tag="ACHA5T_000926"
     CDS             24944..26749
                     /gene="glmS"
                     /locus_tag="ACHA5T_000926"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFM8886522.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
BASE COUNT         7245 a         5735 c         4780 g         9014 t
ORIGIN      
        1 taaaaagact aagaaacaat aacaaaagtt aacacttctt agtctttctt tagcacgtct
       61 atttattaaa gattttaggg ccttttctca tgtttttacg atcattctta ggtgtttcca
      121 tttgtttgga tcggcctcca ccttgtttct ttttgatcaa ttctttcatt ttttcttttt
      181 ggtccatttt tattctcctt taacgaatga agcgactaat tcgatatgat gggtttgcgg
      241 gaaaagatct acaggctgaa ctttcttcac ggcataacct gcttctgcaa ataacttagc
      301 atctcttgca aaagttgccg gattacatga gatatagacg attcgttctg gtgacatttc
      361 gattgctgat tcaataaaat gatgatctaa tccttttcgt ggagggtcaa ctacgatcac
      421 agatggtttg atcccttttt cttgccattt tttcataact ttttctgctt ttccgacttc
      481 ataatgaaca ttttcgattt tattatttaa agcattgaac tgtgcatctt caattgcttc
      541 tggaataact tccatcccat acaccttatc cactctgtct gctaaagaca agccaatcgt
      601 acctattcct gaataagcgt cgatcacaat gtctttttta tctaactcgg caaactcgat
      661 ggctgtttgg tacagatact ctgcttgttg agtatttact tgataaaatg actttgctga
      721 gattcgatag gtttttccta atagctgatc ttcgatatag tctctgccat ataaaacttt
      781 ttctttgtct cctaaaatca catttgtttt ttcttcattg atattttgga tgaccgaaac
      841 cacttctggc aattcttcat gaatagcttc agcaatttcc ttggatttaa agaatttttc
      901 tttacgagta actaaaacga ccatcatttc atgggaataa tgaccacgac ggataatgat
      961 gtgacgaagc tgtccttcat ttttttcttc attatatcct cgtacttgga accgttgcaa
     1021 gatatctctg actactttga ttgcttgatc gatgacagga tcttggatat aaaaatcttc
     1081 gatagggact agatcatggc tattcttttt gtaaaaaccg gtggctaact gtccatcgat
     1141 acggcgaaca gggatctgtg ctttattacg gtagccgaat ggttctgcca ttccgaccgt
     1201 tggaagtaca ggaacttctg gcatcttagc aatcttcgcc attacatgct ctacttgttt
     1261 ttgtttgaag acaagctgtt gttcatactt caaatgggct aaaggtgcga tacctatgcg
     1321 aagtaatgca ttgttttcta caggctgacg atcaggtgat tgtgtaataa atgtttcgac
     1381 ttttccatat ccaaacttat tgcctgcttt tactactagt atttctactt cttctccagg
     1441 gagtgcattt tcgataaata atggataacc atcgattttt gctaccccca ttccttcata
     1501 actcaagtcg acgatttcga ctgtatatcg ttcgtttttc tttactattt gctgttctgt
     1561 catattttgc tcctttaagc ctatttttca atcctactga actctactat aaatcagcta
     1621 attacacaac taaaaccggc actgacctta ctacgttcag caccggcttt tgattaattc
     1681 ttttgattag gtgtttcgtc tgctattttt ccgtcaccgt cgatatcttc atccgttaaa
     1741 cgttcgactt cttttacgaa ttctttgctg acaacctcta attcttcttc atctgtacct
     1801 gtgattgctt catcaggaat ctgatcggta ttggcaaaga actcaatgtg ttgatgaaga
     1861 ttttggaaat gcatcggtgc ttccccgccg tattcaccat ctaagttgat cattactggt
     1921 cgattgatat catcttctgt ttcaacgaac aaactgcttg ttttggtata gattattctt
     1981 ggatcatcga catgtcgacc gccattcagc atgagtgctg caatatgcaa gatctcaaag
     2041 atatttgctg tcttcacaat gattaaagaa aacttcccat catccaattt ggcatctggc
     2101 gcgatctgtt cgaatcctcc aacagagttc gtcagcccta ggaaaaacat cgaagcattt
     2161 ccgatatatt ccccttcatc atacttcaag cgcattttga ttggctttac tcgagggagc
     2221 atctctgctc ctttcgcaag ataagcaaga taaccaaaaa tactctttag ttctgatgga
     2281 acttcataag tcaattctgt caaatagcca cctgcagcga tattgatgaa gtaactgtta
     2341 tcagaaaggc cgatgtccat ttttacggtt tggtgttttt ttatcacttc tgctgctgct
     2401 ttaatattat ctctaggaat ttttaatgct cgagcatagt cgttcgttgt accagctggg
     2461 atgatggcca ttttaggacg gtgttcaagc ggagcgattc cattaaccac ttcgttgatt
     2521 gtgccatcgc caccagctgc cactactaga tcaaaaccta gttctgcgac tctacatgct
     2581 tcattcttgg ctgaatctgg ttctggtgta gttgcatagg cgctggcttc aaatcctgct
     2641 tcttctaatg cctgtaggat atccgccaaa ttttttttca ataattcttt tcccgaggtg
     2701 gggttataaa ttacgcgtgc ttttttcatt ttcagcctct atcgtttcgc taattcatct
     2761 ttaagtagct tattcacgac accaggattt gcttgtcctt tagttgcttt catgatttga
     2821 ccgactagga agccaaccgc acgatctttt ccatttttga aatcatcgac agattgttgg
     2881 ttgttatcca aaatttcatt aatgattggc agtagtttgt tcggatcaga taattgcacc
     2941 caaccatttt tttcaactac ttcttgggct tctccgccat ttgtgatcaa ttcacggaag
     3001 acttttttgg cgatttttga gctgatcgtt ccatcggcga tcaaacgaat cattcctgct
     3061 aagttctcag gtgtcaattt tgtctcagct aattccagtt tctcactgtt caagtaagca
     3121 gaaacttcac ccatcaacca gttagatgct tgcttggcat ctgcaccatt ttccaaaaca
     3181 gcttcaaaaa aatcagacat ttcttttgta agtgtcaata ccattgcgtc atactctgga
     3241 agtccaaact cacggatata gcgttcgcga cgagaagctg gcatctcagg caaactagct
     3301 tttacttttt gaatccattc gtcatcgatc acgaatttcg ggatatcagg ctctgggaag
     3361 taacgataat cactagaccc ttctttgaca cgcatcaaaa ctgttttatt cgttgtttcg
     3421 tcaaaacggc gtgtttcttg ttggatttct cctcctgaaa gaagaacttt tgcttgacgt
     3481 ttttcttcaa aagctaaacc ttttttcacg aaagataaag agttcaagtt tttcaattct
     3541 gctttcgtac cgaactcttc ttgtccataa gggcgaagag aaatattggc atcacagcgc
     3601 atagagcctt cttccatttt cacatcgctg acttctgtaa attggataat tgatcgcaat
     3661 gcttctagat aagcgtatgc ttcttctggt gaacgcatat cagcttcaga aacgatttcg
     3721 atcaaaggcg tgccttgtcg gttcaaatca acgtaagaat aaccgccaat accatgcatg
     3781 ttttttccag catcttcttc taaatggaca cgttcgatcc ggattttttt ctttttacct
     3841 tctacttcga tttcgatcca tccgtcatga ccgattggtt gatcaaattg ggaaatttga
     3901 tatgcttttg gattatctgg gtaaaagtaa tttttccggt caaaatgagt atctttagag
     3961 atttcacagt ttaatgccaa agctgctttc atcccgaatt ccagcgcagc tttattcatt
     4021 actggcagca cgcctggata accccagtcg atcacgtttg tattgctgtt tggttctgca
     4081 ccaaaatgag ctggcgctgg agagaaaatt tttgagttgg tttttaactc gacgtggact
     4141 tctagtccaa tgactgtttc aaaattcatt cgtctttccc tcctaaaata gctggttgtt
     4201 gtttatggaa ttctgttgct tgttcaaacg catatgccgc ttgatacatc gttttttcat
     4261 caaaataatt accgatgatc tgcaatccaa ctggtaatcc ttctgataaa cctgccggta
     4321 cagacatacc tggaagtcca gccaagttga cagggatcgt taagatgtca ctcatgtaca
     4381 ttgtgattgg atcgttaatg ttctcaccga ttccgaatgc aactgttggt gaagatggcc
     4441 cgatgatcaa gtcataatct gcaaagactt tttcaaaatc tcgtttgatc aatgtacgga
     4501 cttgtcccgc ttttttgaag taagcgtcat aataaccagc acttaaggag aatgtaccta
     4561 acatgatacg gcgttttact tcttcaccga atccttctga acgtgagttg acatatacat
     4621 catctaatga tttcacattt tctgagcgat aaccgtagcg gataccatcg aatcgttgca
     4681 agttggaact tgcttctgat gaggcaatga tataataaac agccacaccg tattttgaat
     4741 gaggcaagct gacttcttcc acaacagcac ctaatgaacg gaatgtttct gctgctttca
     4801 atacagcttc ttttacgcca ggggccacac cttcgcctaa gtattcttta ggtaaagcaa
     4861 tcttcattcc tttgatgtcg cctgtcagtc cctctgcaaa atcaggaact gaaacgcctg
     4921 aagaagtacc gtctttttcg tcataaccgc tgatagctgt caatgctaat gcattatctt
     4981 tcaccgtacg ggtcattggt ccgatttggt caagagatga agcaaaggca atcaaaccaa
     5041 aacgtgagac acggccataa gtaggtttca tccctacgat gccgttaaaa gctgctggtt
     5101 ggcggatact tcccccagta tcactaccta aagaaactgg aatttgtcca gctgcaactg
     5161 ctgctgcaga accaccagaa gagccaccag gtacttttgt tgggtcccaa gcattttttg
     5221 ttttcttgaa ataagaagtt tctgtacttc cacccatagc aaattcatcc atgtttagct
     5281 taccaacagg gatcatatct gcttgataga ttttatccat gaccgttgca tcataaattg
     5341 gttcaaaatt gtaaaggatc ttagatgcag cggttgtcaa gatatctttt gttacgatat
     5401 tatccttgat accaattgga ataccagcaa ggacgttgct ttcatcaatc cctttttcat
     5461 ctaaagcttt tgcctgttcc aatgcttttt cttcgttcaa tgtaatgaat gcttcgacct
     5521 caggctctgt ttctttgatt cgttcaaatg ttgcttttgt caaatcttgt acagtgattt
     5581 ctttcgatac aagcatatga tgcagttctt ctagagaacg attgtgtaat tctgtcatta
     5641 tgcgccagcc tccccattat cgatgattgc gggtactttg ataaagccgt cttctgtttc
     5701 cggcacattt ttcaataatt catcgcggtt ccatccagct tgcgcccgat cttgtcgcat
     5761 cacgtttact gtttcaacca cattcgacgt aaatggaaca ccttctgtat ctacttcttc
     5821 cagcatttca accatatcga tgattttacc taattggtcg gtaaaacttg caagttcttc
     5881 atctgcgaac gcaagttttg ctaatttggc aacgtgtttc acttgttctt cactaatcgc
     5941 catactaccc cttctttcta aacgagttaa aaactttgct ctctatttta acacaagagt
     6001 gccgtgtctg aaattgttta ttgaaatttg tctgaaaaaa ctcatttttc attcttctac
     6061 tgagcgactt ttagccaatg catctgccat ttgatcttct gtccaaactt cgattcctaa
     6121 ttcttgtgct ttggtcagct tgctgccagc atcttctcct gctaccacga tatccgtttt
     6181 tttcgatacg ctaccagtca ctttcccgcc aagattttcg atcgtctctt ttgcttcttc
     6241 acgagtaaat cgtgttagtt taccagtaag gactactgtt ttttctttaa aaggagactc
     6301 gacttcttgc aattgggcat ttcgaagccc tttgtattcg aaattgacgc ctgcttgttt
     6361 taattcattc atcaattcat ggacttcttc gttactgaaa taagtgacta cactgtcggc
     6421 aatgatatca ccgatcgtat caagcgcaat aatcgactca tagtcacttt tactcaatgt
     6481 ttctagatca ccgaaatgtt ctgctaaaat ttttgctgct ttcgctccga catgtcggat
     6541 tcctaacccg aaaatcaacc gctcgaccga attatctttg ctgttgtcga ttgctgtcaa
     6601 aatattattt gctgattttt cttttatttt gtccaacgtc aataactctt cttctgtcaa
     6661 tttgtaaaga tcagcaacat ctgctactag ttttttatca tacatctgtt ctagtacgcg
     6721 cggccccaac ccatcgatat tcatcgcatt tcttgaaaca aagtggttca aaccttcttt
     6781 catttgagca gggcatttag gattgatgca ccgtaaggct acttcttcat ccaaatggac
     6841 aagttcactt ccacaaactg gacaatgtgt aggcaattgg tattcttcgc tgtctttagg
     6901 tcgtttatct aacacgactt gcgaaacttc tgggatgatg tctcccgctt tataaatcag
     6961 aactgtatct ttcaagcgga tatccttcaa cttaatataa tcaccgttat gcaggctggc
     7021 acggcttact gtcgttcctg ctactcggac tggtgtcatg atagctgttg gcgttactac
     7081 ccctgttcga ccgactgtcc attcgatgtt ttcgattagc gtttcaactt cttctggcgg
     7141 aaacttgtaa gctgctgccc atctcggtgc tttgacagta aaaccaagtt gatcttgtag
     7201 cgagaattca ttcactttga tgacgattcc atcgatttca tagggcaaat ccacacgttt
     7261 gtcatgatat tcttcaatgt atgaccacac ttcatcaatc gaatgacata gacgtttttc
     7321 ccgattcgtg tggaatccaa ttttttctag ctcttctagc gcatcatact gggtttttgc
     7381 ttgcatagga ccgaaatcag ctactgtgta taaaaaagtg tccagatttc ttttcgctgt
     7441 gattttagaa tctaactgac gcaaagaacc agctgcggcg ttccgaggat tcgcaaagat
     7501 atcttttccc tctgcttctc tgtcttggtt caattggaca aatgaccgtt ttggcatgaa
     7561 acattcgcct cttacttcga tgttcattgg ttcttttaat ttgattggga ccgaccggac
     7621 agtttttagg ttttcagtga tgttttctcc tacagttcca tccccgcgag tcgcacctct
     7681 cacgaaattt ccatcttcat aacgcaaaga aacggaaagt ccatctattt ttagttcaca
     7741 gcaatagtca actacacgtc ccactgcttt ttgtacacgt tgatcaaaag ctatcagctc
     7801 ttctttgctg aaaacgtcgt tcaaactgta aagcggaata tcatgagtga ctttttcaaa
     7861 accttctaat acttttccgc ctactcgttg agtgggagaa tcagacgtga tcaaatcagg
     7921 atattgttcc tcaattgcta ctaattctgc gtattcttta tcataaacat agtcttccac
     7981 tgttggttta tcttctacgt agtattcgcg agaccattgg ttcagtcttg tacgaagttc
     8041 ttctgctcgt ttagcagctt cgtcaatcgt catttcatgt tccatcattt accctccact
     8101 actaagcttt ttcaattggc gcaaaggcag ccaaaagacg ttttactcct tgacttggga
     8161 aagccacatc tagttctaga tccttcgacg tcccgcttac tcggacgact gttcctagac
     8221 cccatttttt gtgtttgact ttatctccag gtttccagct gttattttcc cctccagtag
     8281 cacttttaga gggaacaacc gctgtctctg gctgtttata gctatatgct gtttttttag
     8341 cgaatacagg tgttttttta ggcgtaggac gcatgccttc gatttccagt aactcctgat
     8401 cgatttcttc tacaaaacga gaaggtcggt tatattgcgt ccgtccatat aatgttctgg
     8461 agaaagcatt tgttaaatac aacgcttctt ctgctcgcgt gatccccaca tatgcgagac
     8521 ggcgttcttc ttccagttcg ctttcttcca tcaatgctcg agataaagga aaaaccccct
     8581 cttctagacc aatcaggaat acaactggga attctaatcc tttggctgca tgcaaagtca
     8641 tcaatgtcac ttgggaagca tcttcttcta aattgtcaat atcggaaaca agtgccagat
     8701 catttaagaa gaccgttagt ttttcttctg gtgcatcagc gtcttcttca ttttgctgtt
     8761 cgaattgttt gtcgaattct tgtgtgaccg ttaaaaattc ctccaagttt tcgatgcgtg
     8821 cttgtgcttc taatgtattt tggattttta agtcttctag atatccgcta cgatccaaaa
     8881 cctctttcgt caattctgtc actgtcaagt atgggatcat ctgagtgact tcttggatca
     8941 tctctccaaa tgcacctaat tgctgtcctg cttttccacc gatatttgcc agatccacat
     9001 tttgcgctgc ttcaagcagt ggccattcat gaagagaagc aaaactgcgt agtttttcga
     9061 tagatcctgg tccgatgcct cgtttgggcg aattaacgat acgctcaaaa ctgattgaat
     9121 cttgaggatt tgccagcaca ttcaaatacg ctaaaatatc tttgatttct ttacgatcgt
     9181 agaacttgtg ccctccaacc attttataag gaatatttgc tttcaacaga gtttcttcca
     9241 ttacacgaga ttgtgcgttc gtccggtaca agatggcaaa gtcgccgtaa ttccgatgat
     9301 tgcttcgaat ctcttcttgc atccgatcta cgataaaacg ggtttcgtct cgttcattat
     9361 ctgcacggta gtaagtgatc ttgtttcctt ctttgttttc tgtccacaag ttttttggtt
     9421 ttcgattgct attgttttca atgacttggt ttgctgcgct aaggatgttt ttcgtggagc
     9481 gatagttttg ttctagcaaa attactgcag catctggata atctttttca aaatccaaga
     9541 tgttctgcat atctgcaccg cgccagccgt aaatactttg atctgcatct cccacgacgc
     9601 acaggttcct aaatctacct gccaataaat tgactagcgt atactgtgcg tgatttgtat
     9661 cttggtactc atctacatga atataatgga atttgttttg ataatatgtg agtgaatcag
     9721 gatgttcttc aaataatcga atcgtattca tgatcaggtc gtcaaagtcc atacattgat
     9781 tatattgaag ctctttttgg taagcagcat aacattttgc cgcaatctct tcgtaataac
     9841 tgccttgcat ttttgcaaaa tcctgagggg tctgcaggct gttttttgct tgagagattg
     9901 ttcctaatat acttcgagga tcatattttt tcggatcaat gttcagttct tttagtatcc
     9961 gtttcatcaa ggtcagctgt tcagaagaat cgatgattgt aaagttccgg ttatacccga
    10021 taaaatcaac atctcttctt aaaatacgca cacacatcga gtgaaaagtc gatacccaga
    10081 catcttcgcc tccagatgcg agaatcgcat tgacacgttc tttcatttct tttgcagcct
    10141 tattggtaaa agtaatcgct aggatgttcc aaggattgac ttctttttcc tcaatcaaat
    10201 aggcaatgcg gtgtgtcagc actctcgtct ttccacttcc tgcacctgcc attagcagta
    10261 atggtccgtc agtgtgaaga acagcttctt tctgacgggg gttcatcccg tttaataatt
    10321 cagctttgga tgacattaac aacatccttt cttttatcaa tcttgttaat tatagcattt
    10381 tcttggcttt cattaaacta gctagttcaa aatcatttgt ttctttttat ggatttttcc
    10441 ttgtcaattc gttgatttcg cgctaaatta aaagaaagcg aggcgtttga atggaaaaaa
    10501 aaggaaaaat caagaaatta gataagactc agatatattt tattctaaaa ggaatatttg
    10561 ttggactgct gacaggaatc gtcgttagcc tgtttcgatt atctatcgaa aagctaagcg
    10621 atttagttcg aagtatctat gaaatgagtc gggaacagcc gatttactta attggcctag
    10681 ctatttgctg catccttgca gcatttttcg tgggttatct ggtcaaaaat gaaccggata
    10741 tcaagggaag cgggattcca caagtagagg gacagcttcg cggggaatta tcaatgaact
    10801 ggttctctgt tttatggaaa aagttcattg gcggtgtact ttcagttggt gcgggccttt
    10861 ttctaggtag agaaggacct tctatacagc ttggcgcatc cattggacaa ggagctggac
    10921 aattgtttcg gagtccatct tcagaagaaa agattttaat ttctagcggt gcaagtagcg
    10981 gacttgcagc agcattcaat gctcctatcg caggattact ctttgtttta gaagaaatcc
    11041 atcactcctt ttctccactt gtttggctga catcatttgc ttcagcaatc actgctaact
    11101 tcgtctctct ttactttttc ggcttgagac ctgttttata tctaggtgac cttccttctt
    11161 tgccgttgaa atattatggt tccttagtga tcttaggagt acttctaggc atcttaggtt
    11221 ttatttatca gaaggtcttg ctggctttgc caaaatttta caaccgattg cctttaccag
    11281 cttattttca cgggttcgtt cctttcttgc tgattttgcc aattggttat ttatggccta
    11341 gtactctagg cggaggcaat caaatcgttt tagcttttgg acatgcttcc ttgccaatca
    11401 ttatgattat cggtttgttt gttcttcgat tcgttttttc catgatttct tacggtggtg
    11461 gtcttccagg ggggattttc cttccgatat tgacattggg ggctttaatc ggtagctttt
    11521 atggaaacat tatggttgat ttcgggatgg atccaatcta catcaaggat tttgtcgtta
    11581 ttgccatggc tggttatttc acagcgattg gaaaagcacc gctgacagca attattttag
    11641 tgacagaaat ggttggctct cttaatcatc tgatgccttt aggattggct gctcttgttg
    11701 cttatatcgt caatgacctt ctcggcggta atccaattta cgaatcatta ttggaacgtc
    11761 tacttggaaa tactcagacc gcctcactaa aaggtgcaag gacaactttt gattttccag
    11821 taacggcaga gagttcttta gacggattaa tggtacgaga cttcaattgg cctaaaaata
    11881 tgctgctaat ctctatacgc agaggaaatc aggaaatctt gactcacggt gatacgatca
    11941 tgaatgtagg agatattttg gttatcttga ctgatgaagc aaacttgcca agaatcaagc
    12001 aggaaatcga ggctaaatct tctatcacaa atttgaagca atcattttaa cgaacatgga
    12061 aaaactattt tacaacaact attcatctac actacttgat tctatatgat tctataaaaa
    12121 aagaagagac tgggaaaaac cagcctcttc ttttttattt ctatgcttct gctgctgcag
    12181 gagcttttct caataaacca taaatcacac cagatacgac tgctccgata gcaataaaca
    12241 ccaaatacat gaccgggtga ctcagtaaga atacaacgaa aatcccgcca tgaggagcca
    12301 gtaatttgat cccgaaagct ccgactaatc cgccagttaa agcagaacca ataataaaac
    12361 ttggaatggc acgaatcggg tcagctgctg caaatggaat cgatccttcc gtgacaaacg
    12421 atagtcccat cacgatgttc gtcaaaccag catcttgatc tgttttgctg aatttattct
    12481 taaacaaacg tgtagcaacg aaaattgcca atggaggtac cattcctcca gccataacag
    12541 aagccatcac gacacttccg ccagttgcaa cagtcgtagc taaagttgct gtaccaaaca
    12601 cataagcggc tttgttgatt ggaccgccta aatctgccgc catcattcca gctaataatg
    12661 caccaagtaa agcagcattc gaaccactca tacttgtcaa gaaaccgttc aatgcatcat
    12721 tgattgcttt catagggaca ttgaccagca acatcaagaa accagtaatc agtaagccaa
    12781 acactggata aaagaggatc gttttaattc catccaatga ttttggtaag ttcttgaata
    12841 atttacgtaa aaatacgatg acataaccag ctaagaatcc accaatcagt gcacctaaga
    12901 atcctgcacc gcctgtatta gctaatgcac cagcagcaaa tccaacgatc aaaccagggc
    12961 ggtcaccgat acttgaagca ataaatcctg ctaataccgg cagcatgaaa ccaaatgcag
    13021 cttgtccgat ttggttgaac cagctagcag cttgattgta attccctaga tttgataatt
    13081 gatcatgtgg cacgccaata aattggtcga tcatgaaaga aagcgcaata gcgattcctc
    13141 caccaataac gaaaggtaac atgtgtgata cgccgttcat caaatctttg taaattcttt
    13201 gtccgatagt accatccgca tttccttctt ctgaactgct ttcttttcca tcaccatgga
    13261 atactggtgc agtaccacta agggctagat tgatcagctc ttctggttta cggataccat
    13321 cactgacagg acgattgatc agttcttttc cgtcaaagcg attcatctct acttttttgt
    13381 ctgctgcaac gattacacct tctgcacggg cgatgtcttc agcagtcaat cggtttttga
    13441 ttccttctga accgttcgtt tcgactttga tctccacacc catttctttc gcttttttct
    13501 tcagagcatc ttctgccatg taagtgtgag cgatccctgt tggacaagct gtaacggcaa
    13561 caacgaattt tctttctgga tgagcagaag cttctgcagc ttgtgtttct tgttctgctt
    13621 tttcttctgc ttctttttgc tcttcagctg tttggaacaa gtcttggact tcttctggtg
    13681 tatttgcttc ttttaatttt cctacgaaat ctgcatcaat cagcaatcga gatagtgctg
    13741 ctaatgcttg caaatgtgtg tcgtttgcgc cttctggagc tgcgatcatg aaaaacagat
    13801 aagttggctg tccgtctaag gcttcataat ctactccttt ttggcttttt gcgaataaaa
    13861 ctgttgcttc tttaactgca ctgtttttcg cgtgaggcat ggcgattcca tcaccaagac
    13921 ctgtagaagt ttgtgcttcg cgggctagaa ttccttcttt atatgtttcg atatcggaaa
    13981 tccggccggc atcaaacatt ttttggacca tttcatcgat tgcgcctttt ttatcagtcg
    14041 cttgcaaatc catgatcatc gcttctttga ccattaaatc cttgatattc atgattatcc
    14101 tccttgaaac taatcaattt ttgtgatttc aacttctggc agcaactcat caatcaattc
    14161 gcgagctgcc aagtcatcag aaaaagctgt tgcgcttccg caagcaactc cccatttaaa
    14221 ggcttcgatt ggatctttcg tacggctgta agccccaata aagccggcaa tcattgaatc
    14281 ccctgcacct actgaatttt tcaatgggcg aactaataca ttcgttttat aagttccttg
    14341 ttccgtgaag agcaaagcac cgtctccagc cattgagatc agcacattct gtgccccttc
    14401 agctagcaat tttttaccat acggaatgat ttcttctaca gattggaagg ttgtattgta
    14461 aagctcagct aattcgtgat tgtttggttt gatcaacaat ggtttcttaa caagtgcatt
    14521 ttttaggtca tcacctgttg tatcaatcac gaaatctgca cctttttctc gaatgatatc
    14581 aatcagttct tgataaaatc cttttcgcag gctagccggc gcgcttcctg acaatacgac
    14641 gatatctcct tgtcctactg ttcctaatgc ttgcttcaac tcatcgattt cttcatctga
    14701 aatagatggg cccaaaccgt taatttcagt ttcagtatct gattttagtt tgatattgat
    14761 tcttgtatct tcttttacgg ttgtgaacaa agactggatt tgttcttttt caagccactt
    14821 cgaaataaat tcgccagtga accctcctaa aaaccctagt gctgtagatt gagaatcgat
    14881 ccgtttcaaa atacgggaaa cattgatccc ttttcctcca ggtaacttga agtcttcctt
    14941 cattcggttc aaatcgccta gttttaaccc atctacgcgt acgatataat caattgatgg
    15001 attaagtgtt actgtgtaaa tcattgtatt gcctccttaa ttgttgtctt ttcacggaat
    15061 tcattaacga attctaatgg acaacgctcg gttatgatcg tggctgcttc caaaggtgca
    15121 acagaggtga aggacaactg gttgaatttg gtatgatcaa tcaatacaaa tgtctcttcg
    15181 gaatttttca tggctaaacg tttaacagct gcctcttctg ggtccggagt cgtgaatcct
    15241 tgttcaagat gggcaccgtt cattcccata aatgctttat taaaatgaaa attttttagt
    15301 tgttctatcg cattagatcc taaaacagca ttagttgcca gctttatcgt tccacctaaa
    15361 ataatggtag caagctccaa atcaactaaa tttgccgcat gttgaacgga atttgtcact
    15421 actgtgatct gttttccttg cagaaaagga atcatttcta acgtagtaga gccagcgtct
    15481 aaataaatga catcttcgtc attaatggtt tcagctgccg atttggcgat ttgctgtttt
    15541 tcgtgaacgt ttttgagtga tttttcactc atattctgct caaaattcaa taattgctct
    15601 ttcttagctc cgccatgaac tctttctaga aacccactat tctccagctc ttgtaaatca
    15661 cgtcttatcg ttgattcaga agcattaaaa aggtttacaa gatcttgtga tttcacgatt
    15721 ttctgctgtt caagcaagtc taggattttt tgtctacgtt cctctgtaag cattttcacg
    15781 atcacctcaa tacaaataat agcatatttt ttttctactc gcaatataaa tcagtcaaaa
    15841 acgttcataa aaattataaa tcagtcaaaa aaaaataaaa aaccagtcaa aaagactggt
    15901 tttttatttt tttgtctatc atcaagccgt tttatcgttt tttattttcg cttcttgttt
    15961 ttttcatata aacagaagca ggttttcttt ccttatgaag aagataataa taaagtccca
    16021 tcccgccaaa catgaccaaa aaaccgatga ctgatcgaat aggaaaatta ggaacaaaaa
    16081 agagacagac ggataataat gatgccggaa ccattattcg ataatagagt tgtttatcca
    16141 ttttcttcac ctcatatttt ttgtaaagta atcaattgtt ctaattctcg gtaatctaaa
    16201 aattgcacac gcagatgctg ctcacctttc ttgacatcga attggatact tcgccgcttt
    16261 ttctttattc gtttgaaggt aacatcagag agtctatgtt tgatttcctt taacggtaat
    16321 tctgacaggg attctgaagg aacatagatt cttaaggata aaagatactc ttgatatttt
    16381 tcatctgtta cctctacttc tagatattcc ctaatataat aacaataagc ttcgattcca
    16441 ttatccaaag caacagtcgg atcgataact ttatagcaca tcatgcgcga gcgattctcg
    16501 tcaacccaag gaaaatacgc ctgagcattt agtgttccgc gaacacgcct ttctttataa
    16561 tctccttgtt gaatatcata actttttgtt acaaaaaatg aagcattctt tttaggaaca
    16621 ggttgcattt gtgcattctg atactggaca gattggatcg tatggtcagt gtaaatgtat
    16681 tcaacgaatg gcggtttcaa taaaccgaca ccattgtccc aagcaagatg atcaaaccca
    16741 agctggtccc taaaatagga ttgaaacatt tgattgtttg gcttttgcca gttaaagggg
    16801 tctcgataaa gctggataca tatgacttcg ttttctaatt ccttcacttt ttctacttgt
    16861 tggatgtcga gaaaaatcgg tttagggatg atttgatgat aatagcgact ataatacatt
    16921 tcccaacacg aagtgaaaca taaaccttgg taaaaaagat cgtatcctgg aaattggttg
    16981 caatcgacta ctacttcccc gttttgttgt ttcattttag gtaaatgttc gatttcatcc
    17041 tttttctcaa acaatagccg ttgagagatc tgttttgtat tgtgatagat aaactcgcta
    17101 taagctcgca tataggcgaa caccccatga gcaatcattt ttttctgaat gaagtctcga
    17161 atcaaaaatt gcttttcttt gaaaacagag atagacaata cattttttat caataattga
    17221 ttatcttcca agcgaaaact acattcgttc tcgtcatttc ctacaactaa ccaactttcg
    17281 tctcttaccg atgctagcat tattttttct tgaagctttc gaagatcata cggatccgct
    17341 tgttgtgtga aagagcggat aaatgttggc tggatcaaca gacgtgtcaa aatagcccaa
    17401 tcgcttattt cctgtttttt ttcttcttca tttaagtctg tgagctggat agtcatcaat
    17461 aatttatctt cgccaatcat tttctcacct ctttaaaatc tttttataat ttaattataa
    17521 ccaacgataa agaaaaacta aaactttatg tcttagaaac caatttggac gattacatga
    17581 tagactaagt aaaaagaaac aaagaggtgt ttacaatggg gttgaaattt gaacgtacag
    17641 ataatttaga taaacttttc gaatcatttg ctgtcgatcc ggaaaaaaca gttgcaccaa
    17701 aagaagagat cgatcgattt ctaaaaccac agccacaaaa tgaaaagaaa aaagacaaga
    17761 aacaaaagta agttaagctc aaaaaaagac ttaccagcca aacttttatg aaagagtttg
    17821 actggtaagt cttttttatt ctttttatga ttcaattgct tgttcgattg ctgcccacgc
    17881 ttcttctttt ccctgttttg taacggaaga aaagagaata aacgtgtcat tgcgatcaaa
    17941 atccaacgct tttttgattt cgctctcatg cttgttccat ttaccacgag ggatcttatc
    18001 tgccttagtg gcaacaacaa ttacagggat atcataatac ttcaaaaatt gatacatctg
    18061 gatatcttct tgagaaggct tatgacgcaa atctactaaa gaaacgaccg cacgtagctg
    18121 gtctctagtc gttaaatatg tctcgatcat ctttccccat tttgctcgct cagttttgga
    18181 tacttttgca tacccatatc ccggaacatc gacaaaatgt aatgcgttct cgatcaaata
    18241 aaaattcaat gtctgggtct ttcctggctt accggaagta cgggctaaat tctttcgatc
    18301 aatcaatgtg ttgataaaag aagatttccc tacatttgat cgtcctgcca gagctatttc
    18361 tggcaattgt gtatctggat attgttttgg tgccactgca cttatgataa tttcagcttg
    18421 atggactttc atggtctctc tccttaacaa ggtgatgaat aaagcaagcg gctgtcgcaa
    18481 aagccaaaat gacttttacg gcagcctctt ttcatcaacc cgcttttttc tctgaatctt
    18541 tatagattac tgttggctca ccagacaatt cagctgcttc ttttgtgata atcacttttt
    18601 cgatgttttc atttgaaggg atatcaaaca tcacatccat catgatttct tcaatgatgg
    18661 aacgaagtcc tcgagcaccg gtattgcgtt cgattgcttt tttcgcaatg gctctcaacg
    18721 cttcaggttc gaattcaagt tcggtattat ctagagaaag aagtttttga tattgtttga
    18781 ctagcgcatt tttcggctca gtcaagatcc gtaccagatc atctgttgtc aatttctcta
    18841 aagctgccat aacaggtaat cgtccgataa actcagggat caatccgaat tttagtaaat
    18901 cttcaggaat gatatgctgc atgacacttt cgtcttcaga aagtttttgg ttttgtgtac
    18961 caaatccaat cgtcttttcg cccatacgat tcttcacaat cgtttcgatg ccatcaaatg
    19021 caccacctac gatgaacaat atgttcgtcg tatcaatctg aataaattct tgatgtggat
    19081 gtttacgtcc tccctgaggt ggcacgcttg caacagttcc ttctagaatt ttcagcaagg
    19141 cttgttggac accttcgccg gaaacgtcac gagtaataga tacattttcg cttttacgcg
    19201 caattttatc gatctcatca atatagatga tccctttttc tgcacgttcc acattgtaat
    19261 cagcagattg aagcaattta agcaaaatgt tttctacatc ttcccccaca taaccagctt
    19321 ctgtcaaact tgtcgcatct gcgattgcaa aaggtacatt caatgttttt gctaatgttt
    19381 gtgctaaaaa tgttttacca gaccctgtag gtccgatcaa gcagatattg cttttttgca
    19441 gttcaacatc atctgtgttt tcttgtgctt gctgattgac acgtttatag tgattgtata
    19501 cggcaacagc taacgagcgt tttgcgcgat cttgtccaat cacgtagtta ttcaatactt
    19561 caagaatttc tagtggttta gggacttcag tgaattcgcg tacagcttct tcataaaatt
    19621 cttcatcaat gatttcttta cataggtcga tacattcatt acagatgtag acccctggac
    19681 cagctacgat ctttttcact tcttcttggg ttttcccgca aaaagaacag cgtacggtct
    19741 cattgctact tggattgtca tacatggtct tcacccctta acttgcaaaa agtatgcaag
    19801 aaactatcaa agactttaca gtcttttact atcataacat agtttagtaa aaagaaaaag
    19861 tgtttaacaa aggatcaact gacctatacc tttgcatttt ttactcttaa caaaaaataa
    19921 ggaactgtga caaaatcgtg tcacagtccc ttatttcgaa taaacaattt tctagaagta
    19981 gcttcttcag aaatgattta ttacttgatt tctattcttt cgcttctgtc gctgtacctg
    20041 tgatcaattc gattgctttt ttcattgtca catcatgttc cagcatatct tctgtcaaca
    20101 cgcgtttgat ttgttcgatt ggcatgttgt atgtttctga taattcttgg atttctgcat
    20161 tgatttcttc ttctgttgct tgaagatttt cagcagctgc aattgcttcg atcacaagat
    20221 ttgttcttgt gcgcatttct gcttcacctt cgaattgttt atgcaagtct tcttctgtag
    20281 aaccagttaa ttgatagtac atttcaggag agataccttg acgttgcatg ttgttcaaga
    20341 attcgtccat tgaacgatgt acttcatcat gaaccatcac gtgtggcaat tcaacgattt
    20401 ctgcgttttc aacagccata cggatcgctg cttcatcttt tgcatcgtcc gctgcttttt
    20461 cttttgtttc tactaattct ttgcgatatt tttctttcaa ttcgtctaat gttgatactt
    20521 cgtcatcaac atcttttgcg aattcgtcat ctaattcagg cacttctttt gcttttactt
    20581 catggattgt cactttgaag actgcttctt ttccagcaag atcttctgct tgataatctt
    20641 ctgggaaagt tacatttaca tctagacttt cacctgattt atgaccaact aattgatctt
    20701 caaaacctgg aatgaatgag cctgaaccta attcaagtga gtaattttcg ccttttccgc
    20761 catcaaacgc ttcgccatct acaaagcctt cgaagtcgat tactactgta tcgccatttt
    20821 cagctgcagc atcttctttg ataactaatt cagcttgtgc ttcttgttca cgtttgatac
    20881 gtgcatccac gtcagcatct gttacttcac ggtcttgttt ttcaacaact agatctttat
    20941 attgtcctaa ttttacttct ggtttcactg ttacttcagc agtgattacc cagtcttgac
    21001 ctttttccat gctttcaaca tcgatttttg gttgagaaac aggatcgatg ccagcttctt
    21061 tgacagcagc ttcataagct tctggtaata cagcattcaa tgcatcttca tataaagctt
    21121 cttcaccgta catacggttg aatacttgac gagaaacttt ccctttacgg aatccaggta
    21181 cgttcaagct ttttttcact ttattaaacg cagttgttaa tcctttttgg atttcgtctt
    21241 gcgcaatcgt aaattttaaa acaccatcat tcgtgccttt tttttcaaat gttgcagtca
    21301 tttaattccc tccgagttta gaaattcatt ttatacatca aaaacgtttc atgcatacct
    21361 ttaaatagta cactaaccct ttcaaattgt aaacatattg aatggataga atccttgttt
    21421 ttcataacat ttttatcttt ttcactgata aaatctaatg gaagaagatc atttgctgca
    21481 aaattttttc tctttttttt aaaaaatctt gtttttcatc ttctgtacct tcatacatcg
    21541 tttttcctgt acacgcctct acagtcagtt gaaaccagcg cttcgggtct tcgatgtatg
    21601 tatcatagac aggataaaca attgcagatt gcaacgtgaa ttcttcattt aacacggaac
    21661 taagcagcgc atcttcatta ccgtaacgat ctgaaaatac acataattgc tggtaacttg
    21721 cctgctgctg cggctttggc agatcttttg gtatgacttt gactaacttt tcttcaatcg
    21781 tcaaatagaa aacaggatgg cattcgttca attgcctaag tgtctccagt aattttgccc
    21841 ttgctaaaat atggacctct ggcgatacaa taagtttact cgctgtttgg ataaactcat
    21901 gataaggaag ctgttcgatc tcatcaatag acgcaagctg acgtgcaggt tccaacgaag
    21961 gaagttcctc cgatttattg tggagtgcct tgatcttctg cctctccatt tgtccggata
    22021 attcttctgc aaattggatt ttttcaatga cccgctgctc gaaagcttca taaaaatctg
    22081 tacttgctat caattttcta gcatataaaa aatcttgtgt actgattaac aagtcaaaat
    22141 aaaattcagc aaactcctca ttttgaagat actgtttttc atgcaagagt gcttgcttca
    22201 atgcttcttt tttctcgtcc aactcgtaaa gacaaaaaac aagcaggcta ttagcctcaa
    22261 agctttcttt caactcgtaa gctcgttgaa ataattcttt tgcctgctga tattgctggt
    22321 ttgctagtgc ttgttgtgct actcgcatta aattctcata tttttctgga aaatcaatcg
    22381 gcttcacgca gtgtcctcct cttgctcatg ttcagctcat gttcaatagt agcaaaacaa
    22441 aaaaagcatg acaattaaaa ataactgaag caccctctaa aagctagatt ttagatctga
    22501 cttttagaag gtgctttcaa ctgttactgc tttttattgc tcatcagctg ttgacttcct
    22561 ggttgaatgc tttgatcaga ttgatcgtaa caacacttgt attagtgaaa aaatcgccta
    22621 gatgttgctt tttctgatta aagattgctg gaagataccc taacatcagc aaaggaaatt
    22681 ttagaatgaa ccggcaagca ccttctctaa ctaaaacggt tgaccagcta agttcttctt
    22741 catcaaagct gatgactcga ataccaaaga tcattttccc aatcgtttga ccatgattga
    22801 atttcgtcaa taaaataaaa tacgctagat aaataagtaa ggctgtgaat ccgtagatac
    22861 tcagataaga atctgaaaaa ttaagccaac ctagattttt ggcaatacca agtgttaaac
    22921 gcgtaatagc tccgatacac aaaagatcga ccaagaaagc aaacattcgg ataaaaaaac
    22981 ctgcatagaa atacttagga aaatcatttg cttttggcgg ttcatttctt gtgtccgtat
    23041 actttttcca tttacgttgt ttctttttga tttcttcggg cgtcaaaggc tctctttcaa
    23101 aagagctcag taccttttgg gaaaagttgg agtcaggtcc tacagcaggc tgctgttgct
    23161 tatctttttc ttgttcctta ttttcatttg aaagctctga catgcttact cacctccgta
    23221 ataatacatt gcttttggtg attccgctgt ccctagattc tcaagaacgg ataagatctg
    23281 actagtctca gaaggcttca atccctggaa ttctgctagt ttacttccta accatgtact
    23341 agcaaaccca gatggcgtgg aagaatattc tactagttcc gcatcttcaa gctgttgttc
    23401 ctttcgcata gcttttaaag catcttcagg aaatcccaat tcatcgacta aacctacttc
    23461 ttttgcttgg acaccatcat aaatccttcc atcagcaatt tttttgactt cttcttcaga
    23521 tttattccgt ccctcgctga cgattgctac aaaacgacta tatgcgctgt cgatatatgc
    23581 ttgaagcacc gcttcttctt cttttgtctc tggtcgcgtt cctgaaccca tatctttcaa
    23641 cgctccgctt ttaactgttg tatcttccac acccagtttt tctagaagtc ctgaataatt
    23701 caatcctgac atgatcacac caattgagcc ggtcactgtt tcttctgttg caaagatttt
    23761 atctgcttga gcagaaatat agtagccacc gctagctgcc atgtttttca tgctgacata
    23821 cattgggata tcatgttctt tacgaatcgt atccaatttc ttagcgattt ccgcactttc
    23881 gtagacaccg ccaccaggag aatttacttc taaaaagata cctttgactg ttggatcttc
    23941 ttcaatcgcc ttcagttgtt ccataaaatt tttatgattg taaccttcag aagcaaataa
    24001 acctgaagcc cctcccgcag caatcgttcc atctactgat aattttacga ttttttgcgt
    24061 actgtctccc tcttccaaca catttgaagc tagttcttca tccccatata accaactgtt
    24121 caatccctca atttgatcgt tttcctgagg cttagtcaaa ctagctgaaa ctaacgaaac
    24181 cacaaataat cctgcagcaa tcagaactgc tgcccaacgt ctcttattca ttctttctcc
    24241 tttctaatac aagtttttta tgacgatcac agtttctgac tgctcagaag tgtcgaaccg
    24301 ctctgctttt tcttcccgcg catacccgga aaacgactct tttgtcaatt catcttctac
    24361 cgtaaactca cgagcttcag caacttccaa ggcatcaggt gcgatttctt ggatgctatc
    24421 ccagccagta tctaaaaata ttttcgtatc atctgataca ccagctgatg cttcgaattt
    24481 ggacaattgt ttttttaaca ttcccagtgt catgatctca atttttgaat ccatttttac
    24541 tcctcctcta tccgattact ttctattgta ccgaactcct gaaaaagatg atagataaaa
    24601 acccattatt ttttgacata aaaagagcat ttgacaaagc atttgatata taccaattta
    24661 ttttttattc tttttttgtt gacaagaaca ttcgcaaaag gttatgattt taaatgtttc
    24721 cagataaaaa acaaaagagg aggatagtta taccttttag gatagcgcca ggtctgtgta
    24781 aacagatgac gaggagaggg tttatcgaaa gattcggcgg atggccctag ggactgcact
    24841 ctacagaaaa tccacaaaaa atatctgcaa agataaaaca aaaggatttt cctgcagctg
    24901 gaagcattca tacgaattca ttatagaaag taggatgttt tttatgtgcg gaattgttgg
    24961 aatgattgga ttaaaaaatg taacaccagg attgattgac ggtctagaaa aattagaata
    25021 ccgtggttat gattcagctg gtatctttgt ctcagatggc acaactgatt atctggtcaa
    25081 agcacaaggc agaattcaaa atttgaaaaa caaaataaat attgatacta caggaaatat
    25141 aggtattggt catacacgct gggcaactca cgggcaacct tctgaagaaa acgcccatcc
    25201 acatacttcc caaagtggac gcttcgtgct cgttcacaat ggcgtaatcg aaaactttga
    25261 agaacttaaa attgcttatt tagcaaatga ccactttatt ggtgagaccg atactgagat
    25321 tatcgctcat ctaattgaaa ctttcgcgaa ggatactaca acgcaagagg cgtttttaaa
    25381 agctttgcgg gtcatcaaag gttcttacgc tttcgctttg attgatcgga cagcacctga
    25441 tgttatttac gtcgctaaaa ataaaagtcc actattaatc ggactcggcg atggattcaa
    25501 tgtgattgcc agcgatgcaa tggctatgct tgctcatacg aaggaattcg ttgagattga
    25561 agatgaagag atggttaccg tcacatcaga aaaagttatt attcaaaatt ttgcaggtga
    25621 catcgtagaa cgttcttcat tcgaagcaca agtagattct tcagatatcg agaaaggtac
    25681 ctatcctttt tatatgctga aagaaatcga cgaacagcct attatcatgc gtcgtatcgc
    25741 tcaaaaatac gtaacagaag acaatcgtgt ctctcttgat caaaagctgg tcgatacatt
    25801 gtctgatagc gatcgaatct atatcgttgc ttgcggaact agttaccatg cggggttagc
    25861 aggtaaacag actctggaaa aattgacaca gatccctgtt gaggttcatt tagccagtga
    25921 atttggctat aacacacctc ttctttcaaa aaaaccattc tttattttct tgagtcaaag
    25981 tggcgaaaca gcagatagcc gccaagttct ggtaaaaatc aaccgtctcg gctacccttc
    26041 acttacgatt acaaatgttg ctggttctac tctttcgcgg gaagcatcct ttacactgct
    26101 attgcatgct ggacccgaaa tcgcagtagc atcaacgaaa gcttatactg cacaaatagc
    26161 tgtactaact ttattagcca aagctatcgg tgataaaaaa gaatttgaaa cttctcttgt
    26221 attcgatatc actcatgaat tgagtttagt tgctaatgcg atggaatcgg tcatcgccca
    26281 aaaagattat ttagaagagt tggcagaagt atatctcagc gatacaagaa atgcctttta
    26341 cattggacgt ggcgctgact acaacgtttc tcttgaagct gctttgaaat tgaaagaaat
    26401 cagttatatc caggcagaag gttttgccgc tggcgaattg aaacatggaa cgattgcttt
    26461 gatcgaagaa ggaacaccag tgattggaat catcagtgaa gaagtgactg gtgcgcatac
    26521 tcgaggaaac ctgaaagaag tcgaaagccg tggcgctaaa acactagtta ttgtctcaga
    26581 gggattagaa aaggaaagtg accagctggt gctaccaact gtccaccctt atctaactac
    26641 tctaaccaca gtcgtaccga ctcaattact tgcatattac gcaacgctat tacggggatt
    26701 cgacgttgac aaaccacgta atttagcgaa atcagtgaca gtagaataat aaaagaacaa
    26761 ggaccagtgt caga
//
LOCUS       DAYYGO010000026        26160 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000026 DAYYGO010000000
VERSION     DAYYGO010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.026"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..1093)
                     /gene="queA"
                     /locus_tag="ACHA5T_000927"
     CDS             complement(62..1093)
                     /gene="queA"
                     /locus_tag="ACHA5T_000927"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFM8886523.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            1286..2461
                     /locus_tag="ACHA5T_000928"
     CDS             1286..2461
                     /locus_tag="ACHA5T_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8886524.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            2465..3106
                     /locus_tag="ACHA5T_000929"
     CDS             2465..3106
                     /locus_tag="ACHA5T_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8886525.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            3103..4020
                     /locus_tag="ACHA5T_000930"
     CDS             3103..4020
                     /locus_tag="ACHA5T_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8886526.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            4017..4688
                     /locus_tag="ACHA5T_000931"
     CDS             4017..4688
                     /locus_tag="ACHA5T_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8886527.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(5229..5717)
                     /locus_tag="ACHA5T_000932"
     CDS             complement(5229..5717)
                     /locus_tag="ACHA5T_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFM8886528.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(5918..6184)
                     /locus_tag="ACHA5T_000933"
     CDS             complement(5918..6184)
                     /locus_tag="ACHA5T_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM8886529.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(6184..6447)
                     /locus_tag="ACHA5T_000934"
     CDS             complement(6184..6447)
                     /locus_tag="ACHA5T_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM8886530.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            6968..7108
                     /locus_tag="ACHA5T_000935"
                     /pseudo
     CDS             6968..7108
                     /locus_tag="ACHA5T_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(7250..9073)
                     /locus_tag="ACHA5T_000936"
     CDS             complement(7250..9073)
                     /locus_tag="ACHA5T_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8886531.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(9671..12376)
                     /locus_tag="ACHA5T_000937"
     CDS             complement(9671..12376)
                     /locus_tag="ACHA5T_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFM8886532.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(12682..13629)
                     /locus_tag="ACHA5T_000938"
     CDS             complement(12682..13629)
                     /locus_tag="ACHA5T_000938"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFM8886533.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            13819..14088
                     /gene="rpsN"
                     /locus_tag="ACHA5T_000939"
     CDS             13819..14088
                     /gene="rpsN"
                     /locus_tag="ACHA5T_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFM8886534.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(14264..14626)
                     /locus_tag="ACHA5T_000940"
     CDS             complement(14264..14626)
                     /locus_tag="ACHA5T_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8886535.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(14637..15758)
                     /gene="alr"
                     /locus_tag="ACHA5T_000941"
     CDS             complement(14637..15758)
                     /gene="alr"
                     /locus_tag="ACHA5T_000941"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFM8886536.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(15774..16124)
                     /gene="acpS"
                     /locus_tag="ACHA5T_000942"
     CDS             complement(15774..16124)
                     /gene="acpS"
                     /locus_tag="ACHA5T_000942"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFM8886537.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(16254..17765)
                     /locus_tag="ACHA5T_000943"
     CDS             complement(16254..17765)
                     /locus_tag="ACHA5T_000943"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM8886538.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(18087..19439)
                     /gene="murF"
                     /locus_tag="ACHA5T_000944"
     CDS             complement(18087..19439)
                     /gene="murF"
                     /locus_tag="ACHA5T_000944"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFM8886539.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYLEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(19592..20668)
                     /locus_tag="ACHA5T_000945"
     CDS             complement(19592..20668)
                     /locus_tag="ACHA5T_000945"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFM8886540.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            20864..22609
                     /locus_tag="ACHA5T_000946"
     CDS             20864..22609
                     /locus_tag="ACHA5T_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFM8886541.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(22889..23326)
                     /locus_tag="ACHA5T_000947"
     CDS             complement(22889..23326)
                     /locus_tag="ACHA5T_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFM8886542.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(23310..24683)
                     /locus_tag="ACHA5T_000948"
     CDS             complement(23310..24683)
                     /locus_tag="ACHA5T_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886543.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(25005..25493)
                     /locus_tag="ACHA5T_000949"
     CDS             complement(25005..25493)
                     /locus_tag="ACHA5T_000949"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8886544.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(25515..26117)
                     /locus_tag="ACHA5T_000950"
     CDS             complement(25515..26117)
                     /locus_tag="ACHA5T_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM8886545.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         7504 a         5561 c         4837 g         8258 t
ORIGIN      
        1 ctttgtcata aaatctttgt aaagcttttt tgttattcaa aactagcggc ttttcataac
       61 cttatttcac gaacatcgca tctccaaaac taaagaaacg atagcgctct tcaatcgcat
      121 gctggtaagc agataaagtc aattctcttc ctgcaaaggc actaactaac atgactaaag
      181 tcgattttgg caaatggaaa ttagttgaga aagcttctac cactttgaat tcatagccag
      241 gcgtaatgaa aatatcagtc catccgctgt ctgctttgat ttctccatta aatttcgttc
      301 caatcgtttc taatgttcga attgaagttg ttccgacagc cacgattttc ccgccatttt
      361 tacgtacttg gttcaatcgc tcagccgctt cttccgtcaa acggtagaat tcactatgca
      421 tatggtgttc ttcaatttta tctacgctga caggtcggaa tgtacctaac cctacatgta
      481 gtgtcagata gactaattca acaccctttg cttcgatctc atcaagtaat tctttcgtaa
      541 aatgcaatcc agctgttggc gctgcagcag aaccattttc tttcgcatag acagtttgat
      601 agcgatctgg gtcttccaga cgttctttga tatatggagg caatggcatt tctcccaatg
      661 attcaagaat ctctagaaaa attccttcat actcaaagtc aatgatccgt ccgccgtgtt
      721 ccaattcttc tttcactact gcttgcaatc tgccatcacc aaaagagatt cttgtcccaa
      781 ctttggcacg tttcgcaggt ttgatcaatg tctcccacgt atctccttgt gtattcgtta
      841 gaagaaggac ttctaaatgc gcaccagtat ctggtttttc tccatataaa cgagcaggca
      901 gaactcgtgt attattcata actagcgcat caccaggctg taactcttca atgatttcat
      961 ggaaatgttt atcttccatc tctccagttt tccgatccaa aacaagtaaa cgagaatgat
     1021 cgcggtcttt taatggtgtt tgtgcgatta attcttccgg caagtcaaaa tcaaaatctt
     1081 ctgttgttaa catatcgtca ctcactttct atcatgtgct tcatcattct atcatgtttt
     1141 tcgttctgtt ggcaaaatca gcactttttc acttctattt tttgttttcc taaataaaaa
     1201 aactacccta tttgtatttt ttcattgttt taggcatact agtaatagat agttcttcat
     1261 tgaaaataga aaaagaggtg gcaccatgat tgaatttcaa aatgtgtcaa aaatttataa
     1321 aggtggaaaa gtcgctgtag aggacgtcaa tctttcattt gaaaagggtg agttcatttg
     1381 ttttatcggt acgagcggaa gcggaaaaac gacgacgatg cggatgatca accgaatgac
     1441 tgatccaaca caagggaaaa ttttgattga tggaaaatcc atccaagaaa ttgatccagt
     1501 ggaattgcga cgtcagatcg gctatgttat ccaaagtatt ggattgatgc ctcacatgac
     1561 gatccgtgaa aatatcactc ttgtccaaaa attgttgaaa gtcagtcaag aagaacggaa
     1621 taaaacagct gaaaaaatga ttgatcttgt ggaattgccc cgtgagatgt tagatcgcta
     1681 cccacatgaa ttatctggtg gtcagcagca gcgtattgga gtcgttcgtg cccttgcagc
     1741 gaatcaagat atcatcttga tggacgagcc attcggtgcg cttgatccaa tcactcgtga
     1801 ttccttgcaa gaccttgtca aagatttaca agagcgtttg ggaaaaacga tcgtatttgt
     1861 aacccatgac atggatgaag cgattaaact agcaagccgt atcgccatca tgagtgaagg
     1921 ccgtgtgatc caatttgata caccagaaaa tattttacgt catcctgcaa acgatttcgt
     1981 agaagagttg attggagaag atcgcctact acaagccaag ccaaatgcga caagagtcgg
     2041 cgaagttatg ctaaatacag caatcacgat tacaccggaa aaatcattac aagaagcaat
     2101 caaactgatg agggaaaaac gtgtagatac gttgttagtt accgataatt cgaatgtatt
     2161 aaaaggattt atcgatgtag aaacgttgaa taaaaaacga ggaaaagtat ccagtgtcgg
     2221 cgacattttg aataaacatg ttttttacgt aaaagaatcc gcctatcttc gtgatacctt
     2281 gcaacgcatt ttaaaacgcg ggctgaaata cgtgccggtc gttgatgaac agaacaaagt
     2341 cgtaggtatc ctgactcgtg catcattagt ggatatcgtc tacgatgtga tttggggaga
     2401 agatcccgca actactgatg ttgcagagtc agctactcct gaagaatcta aggaggtctg
     2461 acctatgcag cagttttttc atcagcacgg ttcagaaatt ctctcaaaag gttttgaaca
     2521 tctttatatt tcaggacttg ctttgatcat tggtatcatc attgctgtac cgcttggtgt
     2581 cttactgaca cgttttccaa aagctgccac gattgtgatt ggcttaacca gtgccttgca
     2641 gacaatccct tctcttgctt tattggcatt gatgatcccg ctatttggcg taggtaaatt
     2701 acctgcgatt atcgcactct ttatttattc attattgccg attttacgta atacgtacat
     2761 tgggatgaaa aatgtcgatt ggaattatcg ggatgtggca aaaggcatgg ggatgactga
     2821 gttccaatcc attttttccg ttgaattgcc cattgctatg ccaacgatca tggctggtat
     2881 tcgtttagct gccgtttacg tgattgcatg ggctacactt gcctcttata tcggcgctgg
     2941 tggtttaggc gatttgattt ttagtggact gaataattac caaccagatc tcattttggc
     3001 aggtacgatc cctgtaacaa ttttagcact attggctgat tggcttctag gtctcttgga
     3061 acaccggctg acaccgatcg cacttagaga ggagaatgaa gaatgaaacg tatactaaaa
     3121 atgaccatgg ttcttttctc tcttcttata cttgcgagtt gttccttccc tggtcttgcc
     3181 agcaatacag atgaagacac catttcgatc acaggtggaa tcacttcaga agcacagatc
     3241 ttagcaagtc tagttgccgg aatggttgaa cattatacag ataaaaatac cgcaatcatc
     3301 aacaatctgg ctactacaac tatcaaccat caagcgatga tgaacggaga tgcttcaatt
     3361 tcagcggctc gctacacagg aacagattta acgactacat tgaaccttcc tcctgaaaaa
     3421 gatccgaaaa aagcgtttgc taccgtgaaa gatgaatttg agaaaagata cggtcaaacg
     3481 tggttcccgt cctatggttt tgaaaatacg tatgtctttc ttgttcgaaa agacaccgca
     3541 caaaaatacc atctatctaa agtcagcgat ctgaaaaatg tagcggatga attagtagct
     3601 ggtgtagata cttcttggat caatcgtaaa ggagacggtt atgacggctt ccaagaaact
     3661 tatggcttct ccttcaattc gatattacct atgcagattg gacttgttta tgacgcagtc
     3721 gaagcaggaa aaatggatat tgttcttggc tactctacgg atggacggat cgccagttat
     3781 gatctagtca tgttggaaga tgatcgtcaa ttcttcccac catatgatgc tgctccagtt
     3841 gtagacaaca agatattagc tgatacgcct catttaaaag aagccttaaa taagttaggt
     3901 cataccatca gtacggaaaa aatgcaggaa ttgaactacg aagcagataa taatctagtg
     3961 gaaccatcag tagtagcaca acgtttctta caagaaaacc actattttga agggaagtga
     4021 agcaattgga taaaatgaat gtctttcaac aatttttcta ttatttccag gaaaatggct
     4081 cctatatctt tgcacaattt atccgtcact tcctgatttc tatctatggt gtcctctttg
     4141 cagcagttgt cgggattccg gttgggatca tgatttctag gagacgtaaa ttagcaaact
     4201 gggtgattcg tttggcgaat atcatccaga cgatcccttc tctagccatg atctccattt
     4261 taatcattgg attaggcctg ggagtcaatg tcgtgattgt tactgtattt ttgtattccc
     4321 tcttaccgat catcaagaat acgtataccg ggatgatcca agtagacaaa aacatccttg
     4381 atgtcggcaa aggcatgggg atgactgcta gacaacgact attcatggta gagttaccac
     4441 tctctgtttc tgttatcatg gcaggcataa gaaacgcgtt agttgttgcc atcgggataa
     4501 ccgcgatcgg tgcattcgtc ggtgctggtg gtcttggaga tatcattatc cgaggaacca
     4561 atgcaacaga gggcacatcg attattttag caggtgcatt gccaactgca ttaatggcga
     4621 tcatcacgga ctggctttta ggtattttgg aaagacggct agatccagcc agccgtacat
     4681 cacgctgatg ttattgtaaa aaacaaaagc ttctgcgacc atttgttggt cgcaaaggct
     4741 tttttagttc tgtcattttt aaggcaataa tccactctga ttcttccctc cttatgggca
     4801 aagagttgct tttcttagct atttatccat tccgtatact ccaacttcat actgccaacg
     4861 ggttcatatc tgcatccacc gactaagttt ttttcttatc cgctaaggca ttttgccaat
     4921 ccattttccc atcactcgtc atggaattca taccacatga tctctaaata ctcgtctgct
     4981 tgttctttga attgggcatc aattcttaca tcctttggcg tggctttcat ttaagccttt
     5041 tccatcagtc ctcgattgtt cgatgccttc agctgcatca tcaaaactgg tgactgccgt
     5101 atcggtaact tcgatcatct tttatacaat caggaacttg ccatcctcat ccttggctaa
     5161 aaacatatct agccattcca gcttctcgcc atgggaggtt tgcggttcct aaaaccggta
     5221 cacgattctt atggactgca tccgtcgcaa ctagacttgg cgatacgatg atccttcacc
     5281 agaagagcac ccccgtaaac caattgatcg atatattgcc aataagaaaa gacattggcg
     5341 taaaagatat cgatccctcg tgatgcattg ccgttgatac tgctattcat ggccgaaagc
     5401 gccacgactt taacttcttc cgtttgagtg acattcatgt tcggcaatgt cgctttggat
     5461 acccgctctg ctaaaggaac gacactggta ttgtatttcg tatctgtatc ttttccaaaa
     5521 gaccatgcca acgaatcctc tgggaaccaa taagacgact ccggttgatt gtccattgct
     5581 tttttcaaca tacttttggc tgtttctcga taaacgacat ttccaattgt cttcgcctca
     5641 ctttggcgat aatcgatggt cgttgccact gccaacacta acaacgatgc tccaatttta
     5701 ctgattttcc ttgtcacact tcttcctcct tgttttacat aaacccgtca acattccatt
     5761 cctaaactca aacaatgacc cttcaaaggt ggagatatag gaacaaatta aagaaatcat
     5821 gcaataaatc caagaaggtt ggttttagaa caaaaaaaga gtctcctttt tggggagact
     5881 ctttgttaac tatctaaaaa ggttggctct tctcacttca ctcataatgt gtccaggcag
     5941 aataaatttc tacaacttta ttttcctcat caactttgta aaccactcga tgctgtctat
     6001 tcagcctgcg tgagtatctg ccttcatgtg taggtctcag cttttcaaaa gactgcgtgg
     6061 gcatatatgg atcttccttc aatgtctgaa tcacttcttc aaattgtttc tttaaattcg
     6121 tttgcttgat ttttcttaaa tccactttcg cagagttttt gattgcgact gtatagttac
     6181 tcatcaaaga gcttcccaat caatatcatc gatattcgta aagctactgt cgtctttttc
     6241 gcggtctcgt actttatcta aagtacctgt ttgctcaaga aaaagggttt cttgaatcgc
     6301 tctccagtct tccagcccaa tcaatacagc attgtttcca ctacgatcac taataatctc
     6361 gatttcagta tgattttcat ttacctcttt caataactga taaaaatttt tacgagcagt
     6421 gctaggattc atcgttgtat tagccattgt ctctcctcct ttttctgata taattatagc
     6481 gtacttgatt aagtacgtca aaaaaataca ataaagcact taaattcagc aaacttttcc
     6541 tcttttttac atagagaaaa agaccccttt ttagggagga tctttgtcca cgctcacttc
     6601 ttactccttt acgactagcg gtagccaaac gtaaagtaat tggtaacaac gtatcatctt
     6661 tagcaaaggt cgaatccaaa gcctgtgagt ctatgcaaca acaccaatac acgaaggttc
     6721 ctcgttgcag tgtgacttga ttgatatctt gaataacctg aggatacgca cgaaagattt
     6781 gtaacggtta ttcaaaactg attttggccg tcaagtcttc tactgcctca aaaatgattg
     6841 cctctttcgt ctcctgtcat tcatgttcac tatcgatttg atacttgctt agaccgttgg
     6901 gtttgtgaat caccaattaa aaatggcact gagttatggt aacaaaaaat ggtcacttga
     6961 cgaggctgag attcctactt atgaagacac atggcactta agagtcgagg taaaggaaga
     7021 agctgaaaat ataggaggct gtacattatt tggcaaagga ttcggaaatt atgatcaaga
     7081 catcaaaatc aaaatgtttt acagctaaat aggaagaata ataacatttc atacgctgtt
     7141 cccatcttta atggcttata gagggcacct ttagctgaga ataagacaaa acagaccctc
     7201 tagcttcaat taagagctag agggtctgtt tttgatttta aaacttggtt tatttcttca
     7261 aatggtagct ataggtgctg acaacgatat tttcacgcca agacaatcgc aatcttaacc
     7321 gcaaacttgt ttggttatgg agaatattct gccagcgtct gtttggtaca aattgaggaa
     7381 tcaacacagt agttgtatag ttttgtttcg ctgcattttt acttacgata tctacataac
     7441 ggataattgg attttcaatc gagcgataag aagagtggac gattgaaaaa cggacatctg
     7501 ggaaatgctt tttaaattct gcttcgattt ctttttcttt ttcgacatct tcgtctagcg
     7561 aaacatgcat cgcaacgaca taatcaccga tcgaacgtgc gtaattcaac gcaccgatat
     7621 ttacccgtgt cacattcccg actaatacaa gaacggtatt cccgtcataa tcatgtagtt
     7681 ttgcatcatt ttctaaacga agctgttctg ctactttttt ataatgatca tggatcttat
     7741 aaaaaatgaa cataacgatt ggcataatga tgaagaaagg ccaaatgtcc cctaaccgat
     7801 agacaaacaa aatcgcaatg attgcatatg agatgaacgc acccgtaata ttggcaatcg
     7861 atttgcttaa ccatcttttt ccttcttttt tccatttgac gaccatccct gtttgagata
     7921 aagcaaatgg aatgaacacg ccgatagagt ataacggaat caagcgttct gttgagccat
     7981 ggaaaataaa cagcaatacg atcgaaccta gcgctagtgt gataatccca ttggaatagc
     8041 ctagtcgatc cccacgatct tgatacatgt gaggcatgaa tttatctttt gccagattgt
     8101 atgcaagaac tggaaaagca gaaaaaccgg tgttagcagc aactgctaaa atcagcgctg
     8161 tagcaaattg aaggacgtaa tacaaaatac catgtccaaa gacagcttta ccaatttgag
     8221 acaaaacagt gacttctttt tcaggaacga tcccatacca atagttgata aatgtgatcc
     8281 ccacgaagaa gaaacctaag atcaatgcca tcatcgtcaa tgtagcagcg gcattttttg
     8341 ctcttggctt cttaaagaat ggtacagcat tactaatcgc ctccacccca gttaaagagg
     8401 aagaaccaga agaaaatgct cgtaaaatca aagcgataga aacacccggc acagccgctc
     8461 ctggaagtgc agtagcttgc aatggcacag cacctgtcac gatattatat aaaccaacaa
     8521 cgatcagtaa cgagatcaca aggataaatg tgtacaccgg aaataatagg aacgatgcag
     8581 attccctcag cccacgaaga ttcagcatca tcagtagcaa aacgattgtc acagaaatag
     8641 cgacttgatg accgtagagt gctggcaccg ctgatgtgat cgcttcagca cctgctgaga
     8701 cagatactgc tactgtcaac atgtagtcga tcaataaaga gccaccggaa atcaagccag
     8761 cattctttcc aagattctca ctgctgacga catatgctcc cccgccatga ggataagcat
     8821 ggatgatttg acgatatgat agcgttaatg aaatcaataa aataataaca aaagcggcaa
     8881 tgggtagcga ataccagatg gctgcagcag acaaagcgac taaaacgaca acgatttgtt
     8941 ccgtaccata tgcaatagat gataacgcat cagacgacaa taatgccaaa gcagcaaatc
     9001 gggttaattt gtgctcatca ttttcagcag attttaaagg cttacctact aacaaccttt
     9061 ttaagtaatc caatttctaa actcctttac acacaatttt acttcaagtt ttatagtcga
     9121 aaatttatag taaacaagat gttatagtac gctcactgac cttaaaagtc aataaacttt
     9181 aagaaaataa ttggaaaaaa acagaaaaat atcaagcgct ttttcaaacg ctttaataca
     9241 gcaaaaatct aacatttttt tatgggcttt ttctttgaat tctataataa aaggatagaa
     9301 cttgacttag aaaatcaagt tctatccttt ttgatatctt aacttttgga agagagatgt
     9361 aaggctgctt tctacccatc attcttacta ctcgaagcat agcgcagttg tcacaacctc
     9421 ttgataaacg gtgtttaaaa gcttgcatct gcggtaataa gcccaaccaa attaaaaaca
     9481 taagaagctg tttttatttg gtcgttctta ttacttgatg cagagcagct tttttacaac
     9541 ctcttggtac acggtgttca atcagctgac cctcggtatt agctcaaaaa aacggcaaaa
     9601 tatgaggaac tatttttcca tttttttctc taatactcag gtcaaaacac tgatttcaca
     9661 acctcttttt ttaatgaaaa atatggctct catgcgccgc ttctttgaat ttcgtcattt
     9721 tttcttcgat gaacggatag attgccgttc ctacgattcc aaagacaaca aacaaaccga
     9781 tcatgatcca gattgctttc gtagcatttg gccaataaat tcctcctgca gattcccgta
     9841 acaagtttac cgcatgagta aatggcaaga acggattgat catttggaag aatttccctg
     9901 agacttggat cggatagttc ccgcctccgc cagagatcga cagtactaag atgataatag
     9961 cgatcccttt accgacatta tcgaataatg cagccaagac atagacgatc atcatgaacg
    10021 ctaaggcgat caagacagca aatgacacgc tgtagaccgg attttggaca tctactccta
    10081 gtaagaaaat attccctaat gtaacgatca agctttgcgc aatcgccatt gtcaaaaatg
    10141 tcagcatacg agcaacgaat tgttctcgtt tcgtaaaatt gtttcggtct ttctttccta
    10201 aatagtattc tgtcgtagca acacttgaaa gaagaacagc gccgacccac agacatagtg
    10261 cagtataaaa cggggtgcta gctgaaccgt tatttgcgat tggataaagc gcattggttt
    10321 gtagctcaac aggttgtgta aagaagtcac tttcttttgt tgcgtctagt ttcaataatt
    10381 tgatgacttc gcctagatcg acttcttgtt cgccttttcg gatcgcgtct gctgcttttt
    10441 ggataccagc tttgatattt ggccagtcat tgttgatcaa gtccgcagca gcgttcacag
    10501 ctgtttcgac ttctggcatt ttttcattta ctgttgtcaa tgtaccggtg atttcatttt
    10561 ttagtgctgg ccaatcattt tggataaaat cagcggccaa tcctaatttt tgctgaatga
    10621 tcggtaattc attgttgtat aggtctgccc cttcattgat gccattgacg atggtatcca
    10681 tatttccatt cagcattgta ttggcatcat ggatttcttg tccaattgca ggcatttctt
    10741 tttgatattt ttctaaaatt gccactgcat tcgttacagt tgctttcgtt gagctgagaa
    10801 gcgaatcaaa gtcgatctgg tccgctttgc tcagtgcgcc ttgtgcagtc ccaatcgtat
    10861 cgatgacttg gtctagtaga gaagtgacag tggaactgat ttgacctgta ttgataccag
    10921 caatgatgcc attgatatta ttaacaacgg catcaatttg atctagcaat tcatcaatag
    10981 ccttcgtgtc ttcattttga acagctgtgt tcaaagcaga taggcggttt tgcagattgc
    11041 ttagcaaggg ttttaattga tttagctgat caataattgg ctgtaatttc gtatttccag
    11101 cacttgtttg caatttagtc aggaactcga ccagtttatc gattgcattt tgttgttgag
    11161 atattccgtc aataaagccg ttcaatacat tgctgatgtt tttcttttca tcatcagtca
    11221 gcttaccatc ttcaatagct gttcttacag tatctattaa cgaacctgtc gttgttgcca
    11281 cttgtccaat tgacttcaat gtaacatcta cactgtttgt gatgcttggt atcgcatctt
    11341 tcagctgaga ggcacctgat tttgtttggt ttgctagatc acttgcctga ttttctaaat
    11401 ttttcacatc tggcaagata tcttgtactt gttggatgat cgtcaagcct tgttttgctt
    11461 catcgattcc ttggttcatt gtctcttgga tcgaagcaaa atcgccgtcg atctctgcta
    11521 gttgcttacc ggcattttgg atctctggga ttttttcttg caaatcaagg atgatttttg
    11581 cttgatcttt caactcaggc attttgtcat tcaatgcaac gactttttca cccatctcat
    11641 ctactttagg gatgtaatcc acgaattcat ttgctttatt cagtttttcc ttgatttcag
    11701 gtagttgact ttgtaattcg aggacttgtt ttgtgtagcc gtcgatcgtg tccaaatttt
    11761 catctgttga taaaatcata tctttaactt tattgatact tactaggttc gtatcgatgt
    11821 cgtaacctat ctcattgaat actttcaata aagtcgaact ggctgttttg ataaattcat
    11881 ttgtgatttg cgattggatc gaacttgctc ctttatcggt gattttcggt gcaatcgcat
    11941 tgattttttc atttaccgta tattcgattt ttggtttctt gatatcacca ctagtaaaac
    12001 tcagcagatc ttctgagaaa tcctttggca agtaaatacc tgcgtaatac tttccggaac
    12061 ggactccgtc ttctaattgt tcttttgaat caacgaattg ccatcctaat tgtttatttt
    12121 tatgaagcga ttcgatgact tgctccccaa tggccacttc tttaccttga aactctgctg
    12181 gcttgtctgc actgtaaaca gcaattggca gctctccagt atttccatat ggatcccata
    12241 atgctttgat gttaaaccac gcatatagcg aagggatgat catgatcgcg atgatcagaa
    12301 gtgttgcaat cgggtttttg aaaatccgtt tccagtcaag caaatataat cgaagcgtat
    12361 ttttgattga ctgcatagtt ctcccccttc ttcttttttc agctgaatta ctttaccatg
    12421 tatttcatac ttttttcaat cggctttttt caaaaaagtc gaatttttgg acatatcacg
    12481 gcaattgtct cattgtaatc tcttacatat tgagaaataa ttaaggatta cagcctttac
    12541 tttatcatga aataaaaggg aaagataatt ttatgccaaa gtttttatct attatacgta
    12601 tacgcaaaaa agctaaaaca ttttttgtct ttgcccctac aaaaatgttt cagctttttt
    12661 cgttttttat tcctgtattt cttatttttc gaatgacagg atgattttcc cagcaggagg
    12721 atgtccttca actagctgat gtgcttggat gacatgctta agtgaagcag acagattcat
    12781 tgaaataaaa acatgaaagg ctcctttttg gtaggctttg attatgccag caaaggcttc
    12841 tgcatctaga tattcctttc ttggaacaaa cgattcataa aagccttcct ttttttgccg
    12901 caaatctaga tctgggaggt catttaatgc aacatacctg ccgcctggtt tcatgatctg
    12961 gatgccggtt tctcctttga tacttccttt cgtcgcatcg attacaaggt ctgcctgatc
    13021 agcaaattgc agacctggat ttgttttatc ataagcagca aatgctgaag ctcctaattt
    13081 ttttaccttt tcctcatttt tactggatgc actggttaag attcggattc ccttttcatg
    13141 gagaagctgg atcagacttg acccgaccgc acctgatgct cctagtatca ttacagtatc
    13201 tgtcggctgg atttccgtca aatggttgat tagattataa gccgtaattc ctggcgtgac
    13261 cattccggct gcttcttccc aactcatttt atctggaatt tttgctactt ttgctgatgg
    13321 caacacgact tcttctccgt aagtcccgcc gactgcatgg acagctactc gatcacctac
    13381 atgaacagtt gtgacatctg aaccaatctc tgtcacaata ccagcgccat cattacctgg
    13441 tacataaggg aatttcaatg ttcgtatctc tttcatttct cctaaacgca aggcaacatc
    13501 ataaggattc acactgaatg cttttatttc tacacgaaca tgtccagaac cgacttctct
    13561 gggatgagca tcgatttctt caaaaacatc ttgtgcttct ccgtaaccgt taattgcaaa
    13621 tcttcgcatg gtcaccatct ccctttttct ttaagtttac gtttatgcct ttattttgac
    13681 aaaaaaaatg tcttttcgtt ttggcgcatg attcaaaaca aaatagttgt cttttttcgc
    13741 gtaacgtgtt aaattattta tctaaaagcg taaagattac gatttacgct aataaaaaat
    13801 aattggagga atttgttgat ggctaaaaaa tcaaagattg ccaaagcaaa aaaacagcaa
    13861 gaaacgattg aacgctatgc tgctttacgc caggaattaa aagcgcaaaa agattacgaa
    13921 ggcttgcgta aacttccttt agatgctcgt ccagaacgat tgaaaaatcg tgatttaatc
    13981 gatggacgcc ctcgcggata catgcgtaaa ttcgggatgt cccgtataaa attccgtgaa
    14041 ttagcacata aaggtttgat tccaggagta aaaaaagcca gctggtaatc caagtctggc
    14101 tgtaatgtaa aggaggaggg cgcatggacc actccttgga tcatcaaaaa agatacgttc
    14161 gcttcttcta gcagaaatgc tggtactaga agcagacgta tcttttttta cgcacagctg
    14221 gtcctttttt atatgtaaag gctgcatggt tacttcgcta tatttattta gtaacaggag
    14281 tcagtgcttc gatacccaca ctaattttca atgctcggtc aacttctgcc ataatgggtc
    14341 gatccaaatg gcagaccttt tctttcaatc ttgatttatc gatcgttctg atctgctcca
    14401 acaggataac ggagtctctt tctattcctg ttccagtagc tttgatccca atgtgagtcg
    14461 gaagcttagg tttagccatt tttgccgtta tggccgcaac aatgatcgtc gggctaaaat
    14521 ggttacctag attattttga ataaccaaaa ctgggcgtgt tccgccttgc tccgatccca
    14581 cgactggaga aagatcagca aaataaatat ctcctctttt taccatagga tcctccttat
    14641 tcttgatatt ctcttggtat ccgctgtcct aatccgcaag ccacttcata gtggatcgta
    14701 cctaattgat ctgctatatc ctgcatagtg atttctttgt ctgcattttt gccgataagc
    14761 gtcacttttg ttccgacagg aaattcttga ggcaatcgga tcatcagttg atccatacat
    14821 acacgcccaa tcgtttcaca gtagttccct tctaccaata gagaaaaacc ctgcatcttt
    14881 cgtggccagc catctgcata accgatcggt atcgttccga tccactctgc ttcaggagtg
    14941 atatatgttt ctccatagcc gattccttca cctgctggga gttttttgac ttgaatcaat
    15001 tcagaaacca gctcaagtgc tggctgcaat ggataaactt ccggtagtgc gtgcccagag
    15061 ggattaagtc cgtacattgc tactccataa cgaatcatat tgccgattgt ttcatcatgc
    15121 cataaagccg ttgcactatt gctgacatgt acatatcgag gaagttcggg aagttttttc
    15181 agcacttcta taaagcgttc tttttgcaga ttccaatatg tatcatctgc ctgatctgct
    15241 gtagaaaaat gggtaaataa accttcccat tctaatacac gactttcttt gatgaatgca
    15301 gctgcctctt ttacttcttc aggtgtacaa aatccgatac gacccatccc agtgtctgct
    15361 tttagatgaa tcgaaagagg cgtctctagc ttacttttct ctaaaacagc agctgctgct
    15421 tgcaaccatt cccgtgtacc tgcagttact gataaatcat gagcaacagc tacagtcagt
    15481 gcgtcgtaag gaaccatatt caagataagg atgggctgag taaatccagc ttctctcaac
    15541 tcgactcctt catctaaatt tgaaacacaa aagcccgaag ctccacctgc aactgctgcc
    15601 tcagctgttt caatagctcc atgaccgtat ccgttcgctt ttactactgc aaacaattct
    15661 tttccttgcg gaagtcttgc aacttcattt gctacatttt cagttatagc ttttttatgt
    15721 atcactgctt tcgttgggcg gtgccaagcg acaaccatcg tttttccttc tttctagtct
    15781 tccaaaataa tttgtgcaaa agctgtttca tttgtatgag taatactgac ccatacattg
    15841 ccttcatgag gtgaacgcgt cacaacaggc tgaccatttt cattttttaa aatctcgaca
    15901 tcttgaaagg taacttttcc gatcccagtt ccccaagctt tagagaaagc ctctttgcaa
    15961 gcgtaacgcc cgcctaaaaa ttctacttgg cgatgaaaag gcaaggactg gaatagtttc
    16021 atttcatcag aggtcaagat tcgtgcaatg aattttggtt tttcttcgat cagccgagtg
    16081 atcctaggaa gttcgactgc atcgatccca attcctttta tcatatgctt ttctcttttc
    16141 aataaattta gaacactctc attctaccaa attataacca ttttgccaca aataagtcca
    16201 caaataacaa aaatgaaagt ttctcttaaa aaagagaaac tttcatttta tgtttaatcg
    16261 ttatttgaac ggattacaaa tccgcgtttt ttatcattgt gacgtttacc gccatctttt
    16321 tggctgttat tatttttgct ataaccgcct tttgatttgt ttccacgata attaccgccg
    16381 tcacgacgat tacggttgtt tccgccacca cgtttgccat ttttattgta tccttttttc
    16441 tgcattggca atgggcgttc tggtgtgatc ttcactggca ctgcatcagc tggatctttt
    16501 gctgtcgttt ttagcaaaag tgcaactaaa tcttgtgcag aatattcttc taataattta
    16561 tctgctgttt gcaggtattt atctagacca ttttctgcaa gttttgtttc gatttgttca
    16621 actgcagcac ctaattgacc tttgaatgct tctttttcgg ttggtggacg taaagtcgtc
    16681 atccgttttt tcgtcaagtt ttcaatcaca tgcaagtagt ccatttcatt tggtgtaacg
    16741 aaagtcacag acataccgcc ttttccggca cggcctgtac gtccgatacg gtgaacgtag
    16801 ctttctggat cttgagggat atcatagttg tacacatgag tgacacctga aatatctaat
    16861 ccacgtgcag caacgtctgt cgctacaagg atatctaaat gaccgctctt aaatgaacgc
    16921 aatacgctca ttcgtttttg ttgagacaaa tcgccatgga ttccttctgc acggtaaccg
    16981 cgtgcttcta atccgcgtgc caattcgtct acacggcgtt ttgtacgtcc gaagacgatt
    17041 gttaattcag gtgtttgaac atcaaacaag cgagtcatga tatcgaattt ttcgtattct
    17101 tttgcgcgga catagtattg atcgatcaag tcagctgtca tttctttcgc tttgatcttc
    17161 acatgatgtg gatttttcat gaatttaacg ccaatattct tgatagctgg cggcatcgtt
    17221 gctgagaaaa gtaatgtttg tctttgatca ggaacttgtg aaatgatttt ttcgatatct
    17281 tccaagaagc ccatattcaa catttcatct gcttcatcca aaactaatgt ttgaacggtt
    17341 ccaagtttca atgtgtggcg gttaatgtgg tcaagcatac gtcctggtgt acccacaacg
    17401 atatgtggac gatctttcaa gccacggatc tgacggccaa tatctgctcc gccgtaaacg
    17461 gcttgtacac gtactttttt gtcgcgccct aaacgatata attcttcttg tgtttggata
    17521 gctaattcac gtgttggtgc gataactaaa ccttgtaatt cgtgacgatc tggatcgatt
    17581 ttttctaaca ttggtaatcc aaatgcagct gttttccctg tacctgtctg tgcttgtcca
    17641 ataacgtctt ttccagctaa tgctagtgga atcgtttctg cttggatagg cgttgcttct
    17701 tcaaaaccag cgcgttcgac tgatttcaac aattctggtg ataattctag ttctttaaat
    17761 ttcaaatgaa tcctcctata tggttgttaa ctgaggatgt agcttttttg ctaccccgtc
    17821 gatttgttcg gtcaaagtgt attgtttctt tttgacagtg gccacgcacg tttttatcca
    17881 ttcttagccc ctgtggcacc accgaaaaat cactttgcga agtttaggca ttagaaaagc
    17941 caaacgggtc gcgttcagct ttatcattat tatgacatct ggcttcaaca agttgtttct
    18001 atggcaaaac cagtccaatt tctcttcatc tttcggcaat aggttattct aacatagttt
    18061 ttcgttttct gcaagtttac agatttttat ttcatttgct gaagttgttc taccacttca
    18121 aataagccca tgctgttgct gccttttagc ataatactat ctgttgcttc aagattttgt
    18181 ttgatagcat caatcaattt attcttttct ttttccaaat agaacacagg tagttggggg
    18241 tatttttggt tcaattcttc ttttagtgca tgcatttgtt ccccataaag gaaaatcatg
    18301 gcgaatttct caggatcgat atgttcactc atttgcgcat gcatatttaa agactctttc
    18361 cctaattcca gcatatctgc taacacagcg attctgcgtc cttttgttgg cagattttta
    18421 aaagtatcta ataccagtcc catggctgtt ggatttgcat tgtagacatc gctcaaaata
    18481 gctgcaccat ttcctgccgt tagccattcg gttctgtttt gagtgataga aacatgggcg
    18541 agcccttggc gtatctcagg tatggacagt ccaaagaagc gcccgatcgt atacgcaatc
    18601 aaagcattcg ttacattata acttccaggc agcgggatcg taaatgtttc tccttctact
    18661 acaaaatcgg tttgtgcttt tccttttgct gtcacttccg catgaacatc gccatttcca
    18721 ataccgaatg ttgtgactgt ttgtactaat gttgccgtta aaggtgtcag caacggttca
    18781 tctgcgggca caacgagtaa tccgtctgat acaagtccgt cagtgatttc cattttggct
    18841 ttagcgattc cttctcgcga cccaagattt tctatgtgtg cttcaccgat cattgtgatt
    18901 gctgcagctt ttggttgagc cataagtgaa agttccgtga tttcattggc gtgatccatg
    18961 cccatttcca aaacgatctt ttctgtatct tggggcatgt gcaaaatcgt gtaaggaaga
    19021 ccaatattat tgttgtaatt tccttgcgtc ttatatgttt tgtatttttc agacaagaca
    19081 gacgcggtta gatctttcgt ggtggtttta ccattgcttc ctgtaattgc aacgacttct
    19141 gcgcctactt tttccaagta ataggttgcc aattgctgca tggctttcaa aggatctgct
    19201 acaggaataa ctggaaaatt ttttggagct tgttcaacag gcaagctcca aaatgaagca
    19261 gatgctccgt ttgtgatcgc ttgctggata aattcatgtc catctcgcgt accagcaaga
    19321 ggaacgaata aacttccttc ggttatcttc cggctgtcaa attcgacatt ggtaatcttt
    19381 gtcagtgcag agcattcgct gcccacggct tgtgcaattt cttgtattgt tagattcatt
    19441 gagatctctc ctaaaaaagg ttgtgaaaaa agcgtttggc ctaaacggcg ggttcgcatg
    19501 actctcactt ccaagtaaat ttctctattc gtatttagaa gctagaatga acgcggttac
    19561 gcaatcactc cagcctcttc tttatttctc tttattcatt tttttcaaaa aaagattgac
    19621 gctgatggta tcgattcatt cctaactgga tcagttcttc aatcaaatca ccgtatttca
    19681 agcccatatt ttcccataag agtgggtaca tactgaactc cgtaaatcct ggcatagagt
    19741 ttaattcatt caggaataat tcatttttat ttgtcaaaaa gaaatcgcac cggctcaatc
    19801 cgcttccacc taacatcgtg taagctaact tcgcgtactc ttgcgctttt tgataaactt
    19861 cttctggcac ttcggctgga atctgcattt cgattttatt attgatatat tttgcttcat
    19921 aatcatagaa tgctacgtct tttacgactt caccaggcaa agtcgtccga acgtcttcat
    19981 ttcctaatac agcaacttcg atttcgcgcg cttcaattcc ttgttcaacg attgctcgag
    20041 aatcatactg ataggctgtt gctaaagcat tttgcagctc ttctcggttt tctgcttttg
    20101 taatgccgac actagaaccc atattcgccg gtttgacaaa catcggataa agcaaagaac
    20161 cttcacattg atcaaatact tttttaggat tttctttcca ttgattctta agtactggta
    20221 cataaggaac ttgcggcaca ccagcagctt gtaaaatata cttggtcatg attttatcca
    20281 tggcacatgc actggtcaat acgcctgcgc cgacataagg catattcaat gtctctaaga
    20341 agccttggat cgttccatct tccccgtttg gcccatgtaa aactggaaaa acgatggctc
    20401 cttcttcttt gatttcgccc ggattgatca cttttcctgt aaagccttcc tctgtctgtc
    20461 cacttgggtc ccatgaaaga tgcaagacat ctttgctggc aggtttttct gttagtaatg
    20521 gacctttgac ccattgtcct tcttttgtaa taaatacgag ttgaacttgg taataattat
    20581 aataaatggc atttaaaact gaaaatgcgg aaagaatgga cacttcatgc tctgcgctgc
    20641 gtccgccata tagtaaagta atcttcaaaa gattgtcctc ctgtattcta aatcgttcag
    20701 tgatttactc tcatatttta gcacaaaaca aaaataaacg aataacttat ttataaaaaa
    20761 acgagccagc ttctaaaaga acgagcgtca tttatttttt gtcagctaga ttatttgtcc
    20821 cgttttttat aaaaagttat gataaagaaa aggagggatg atgatggcgc aaacaaatcc
    20881 acgcagccgc ttggcagaaa tcattcgagt gttcatcaat tacaatgtag tgccaaactt
    20941 tgtacagcaa aaaaatcccg aacaggtaaa aaaagccttt gaagaactag gccccacctt
    21001 catcaagata ggacagatgc tttctgttcg agaagatttg ctttcttctg ccttcactca
    21061 aacattcaaa acattacaag atagtgtacc tagcgacaca ttttcaacgg tcaaaaagac
    21121 gatcgaaaca gaactttctc tttcattatc agatatattc gatgattttt caaaatcgcc
    21181 ttttgcctct gcttctatgg ggcaggctca tcgtgccaag ctgaagaacg gtgacgcggt
    21241 agtcgttaag atccaacacc ctaacatcgc cgaagagatc cgacttgatc ttcaactatt
    21301 tgaacgagcg atccctttga tcaaatacat tcctgaaact agcgtagttg atctaaaagg
    21361 ggtactccaa gaagtaaaac gttccttgat caatgaaatg gacttcttaa aagaaagtca
    21421 aaatggcgaa caattctatc aaaagaacaa tggctggaaa gagatccgca gccccaagat
    21481 ttatgacgct ttttgttcga aaaaagtgat cgtgatggaa gaaatgtctg gtaaaaactt
    21541 gaatcattta atgaacgccg aaaacaaaac tgaaactttt atcactggaa ttcaaaacaa
    21601 gcagctgaaa caagaagttg caaagctttt agttgagaac tttatgaaac aggtgtttga
    21661 tgatggtttt ttccatgctg atcctcatcc agggaatctg ctattccatg tactaacaaa
    21721 agaagaacag acacaagcta gccgaaaaac tgaaacagtc catgaaaaag agttcggttc
    21781 ctttgctttt cgagcctcca cttctgcaga agaccctgtt gcgccatata ccatcaacta
    21841 catcgatttt ggtatgatgg ggcatctttc tgcaggactt aggcaaaaac tcacacaagc
    21901 cgttttagcg ctgtatacga aagatgctta tcgaatcgaa aaagcggtcc ttcgactttg
    21961 tcagcaagaa ggctcgtttg atgaaagtcg gttccaccaa gaactgacaa gttttcttga
    22021 acagtattat gattctccta ttgatgaaat caacttgcaa gaagtattca cacatgtcgt
    22081 gacgatttgt catcaaaata atctgcagtt cgatcgtgat atcacgcttt tgctgaaagc
    22141 atttgggaca ttagaagggg tgatccgcgt cctcgatcct gaagtttccc tcatggaagt
    22201 cgcttcgcct tttgctcaac attattttct cactcatctg gatgtagaag atacattgaa
    22261 acaaagcgga ttagacttat tggaagggat gaaggctgcg cctaagatcc ctcaacagct
    22321 ccaccatcta ttagaaatgt ggacttcggg acaaggaaaa gtcaatttgg aattgaaaaa
    22381 gcaagacaaa ttattgagca ggatcgaaag tatgatcaat cggcttgttt tcggtatgat
    22441 tttagctgca ttgatcgtag ggtcctccct gcttgtccaa gcagcacctg tagaaaacgc
    22501 agaggtcgtt tccttattag ggatatttac gtatgctatt gccgcattcg tcattatttt
    22561 tttggcaatc gatgcattga tccaaatgta taagaagagg aaaaagtaga aaaaggttgt
    22621 gacagaaaga atcagcttca agaaataaga aggggtttta cataaattgt ttctcaaatt
    22681 tttgtgaaaa ttcggcttat ttttcgaaga ggctacttct gaaacaccga gaataaaaaa
    22741 tgtgaaaaag ctgttctgca cccagtaata agaacaatca aaaagggaac atggaacaca
    22801 tttatcctgt ttccctgttc cctttttgat gtattcgagc ataaataaaa taccttcgtt
    22861 acctactatc ttctgtactt ggtaaacctt agtcaattgc cttttttaac gcttgacgaa
    22921 aagacatatg atctttctcc gtaaagggtt ttggaccctt tgtccttatg ccagcttttc
    22981 ttaactttcc actatgataa cgttgttcta gaagcgtgcc gatattcgct tcgtcaaatt
    23041 cttttccaga gtgatggaac actcgcacac ccttcggcaa tacaagtgaa gctaaaccat
    23101 aatcttgcgt gatcaaaaag tcattcttct taaccatacc gacgatttta taatccgcac
    23161 tatcagctcc gcggtctacg tagacgattt ggacatgatt agcgtattct ttggtcgtat
    23221 aatggtccac actggtcacg ataaccacgt caagaccata ggattctcct aattttatga
    23281 catcttcttt tactggagaa ccatctccat caatgaatag cctcattctt tttcgccttc
    23341 ttccataaat gataaacagt tactaatccg ctcagtaaaa gaccaacatt gatccatttt
    23401 gccaagataa gcagttgtac actttctatc ggcactgatt catggttcag cgtgatcaat
    23461 aatagatgga ttggctctcc catttctttt gctaaaacaa acaaaagcgt catcaataaa
    23521 aactggtgga tcacactccc tttattttga gtagtatact tgacaacgat ccggcgatcc
    23581 attggaatag ctgtatcagt ttgataatat tccttattta gccagctgcc tgttactacg
    23641 tgccagaaac taagcagaaa gacacctatc gcaaaaccat ttggaatcaa gaggaccaat
    23701 aaagaagaac tcagtccaag caattgcata gccaatggag accatgttaa acgtttcaaa
    23761 atccatggtc cgactatttt tgctgcaatc aagcctgctg cataaggcaa atacataaaa
    23821 atcgaaagtt tttctaatcc gtacagtatg ccaacataaa aactgctaaa taaaaaaagg
    23881 aaattcccta gcattccatt tagaaaagta aacagattta gccagcttgg caacttccat
    23941 tccttcttct ggtgtcctaa aacaaacacg aacaagatga acagcagcaa gaaaccataa
    24001 atagcaagat caaaaagcct ttcatcgaat aacaatcggg catttctcaa aagaaacaat
    24061 aagccaaaga atatcaaaaa taaaacaagc tctcgcgtag ctaccagatt tcttttgatg
    24121 tctttgaaat ccagttcgta acgcggataa tgactaaccg tatggtaggc catgacgtaa
    24181 aaaagtgtat aaatagtaaa gcttgctgca atctgagtcg gtgcaggcaa ctcaatcgct
    24241 tgatacagga gaaatagcca gatagctgca aataagtact ttttcccatc catattgata
    24301 aaaccttgcg tcttttcgtg aaaattgacc gtggtattag caggtacaag ccatgctgca
    24361 ctcagcccta gcaaaacact tgaaaaataa aataaaggaa aataaaatac gcctaatatt
    24421 ccaagcaatg aaccagcacc accaatcagc agagcgatca tcagcaacgt gtaactatct
    24481 agaccaaatc gtagactgtt gactaaaaat agtccagtca tccgaaaggc ataaaacagc
    24541 gtaaatggca aaatcgtcag ccaattatgc gagtctgtta gtcccaaaaa taaaagataa
    24601 ggaataaaaa taaccgtgtt acttaagata aagggcgttg tttgtgtaaa attacgccca
    24661 aatcgtttga gtttttcgaa catgacaatg gctccttaaa cagtcttata actaatctgc
    24721 tcttcgattt tagcattaaa ggggaaataa accaatttta cagaatatat tcttgtaaaa
    24781 aaaaatacag aaagaagcat aagcttcttt ctgcattttt acggtacttg attactcaag
    24841 cagacaagcc cttctccaca aaagtcttgc ccgacacccc tgcatccggc aggtgataaa
    24901 gatagaaatc aattatcctg tgcctccaaa atcaattagg agctctgtaa accagagtcc
    24961 ttctcgaccc gtcgcataaa tttactttaa cacagttcct atgtttatgc gagagctttt
    25021 ttcattttat aatcgaccat ttgaaatcct tgaccgatat ctgaaataag ttcttctgta
    25081 atctcaaatc cttcatgctt ataaacctcg attgcttgat gattcccctg attcactcgc
    25141 aaaaagatct ctctttcctt tgtttgcgca gcaagttctt cataccagcg aaaaactgaa
    25201 gaagtcaatc cttgaccacg tactttattt agcaaatata atttacttaa ataaattttt
    25261 ccatttatct cttcataagc agtgtaaccg acagtctttt cttcgctttt taataaaaag
    25321 taggacacac cttctgtcag ttctttttga atctgttttt ttgactgata agttttcagc
    25381 atgtaatcca cttgtttttg accaatgatc ggcacaaaag cttccggcca tacttcttgt
    25441 atgactgcca tcagctcatc cagttcattt tctgttactt ttattttttc catacaccca
    25501 cgtccttttt tacattataa atgaccttgc gcttgatgga ataaaaagag catgatcatg
    25561 ctatacacac ccaccgacca tggtttcaag aaaatcacaa tctgcataaa ttccctaaaa
    25621 gataatttcg tcaaaccagc tactagacag atcaagtcgt ctggcgcaaa gggaagcagc
    25681 aaggtaacaa ctagtacttt tttaatatct ttcgtttttc ttgtcagcaa ttcatcaaat
    25741 ttctgatact tttttggcga taaaataagc tggacaaagg ctacgccata gtaacggaca
    25801 agcaaaaatc cgattgcttc cccaatcact aggccaatac aactatataa aagcccaaat
    25861 ccattgccaa acatcagcat gcccgctact gaggagatcc ctcccggcaa aataggaatg
    25921 atcacttgga tcacttgcaa cagaacaaat atcatcactg catacttgcc aaattgctgg
    25981 ataaacgctt gcaaagacgt gctcgatcga aaaatcccat gatgataagc ataaagaatc
    26041 aacacaaaga aaaaaaacag accaataatg ggcatgatct gaagacattt ttgtaaaact
    26101 gatccttttt tagacatcta tattccccac atcttttttc atttattaaa actgcctcct
//
LOCUS       DAYYGO010000027        25680 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000027 DAYYGO010000000
VERSION     DAYYGO010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.027"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            279..1127
                     /locus_tag="ACHA5T_000951"
     CDS             279..1127
                     /locus_tag="ACHA5T_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFM8886546.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(1298..1639)
                     /locus_tag="ACHA5T_000952"
     CDS             complement(1298..1639)
                     /locus_tag="ACHA5T_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFM8886547.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAVEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            1725..2432
                     /locus_tag="ACHA5T_000953"
     CDS             1725..2432
                     /locus_tag="ACHA5T_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFM8886548.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            2419..2742
                     /locus_tag="ACHA5T_000954"
     CDS             2419..2742
                     /locus_tag="ACHA5T_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFM8886549.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(2831..3868)
                     /locus_tag="ACHA5T_000955"
     CDS             complement(2831..3868)
                     /locus_tag="ACHA5T_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8886550.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(4315..5298)
                     /locus_tag="ACHA5T_000956"
     CDS             complement(4315..5298)
                     /locus_tag="ACHA5T_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFM8886551.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(5714..6484)
                     /locus_tag="ACHA5T_000957"
     CDS             complement(5714..6484)
                     /locus_tag="ACHA5T_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8886552.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(6477..7412)
                     /locus_tag="ACHA5T_000958"
     CDS             complement(6477..7412)
                     /locus_tag="ACHA5T_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8886553.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(8006..9655)
                     /locus_tag="ACHA5T_000959"
     CDS             complement(8006..9655)
                     /locus_tag="ACHA5T_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFM8886554.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(9850..10746)
                     /locus_tag="ACHA5T_000960"
     CDS             complement(9850..10746)
                     /locus_tag="ACHA5T_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8886555.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(10739..11419)
                     /locus_tag="ACHA5T_000961"
     CDS             complement(10739..11419)
                     /locus_tag="ACHA5T_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8886556.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(11420..12202)
                     /locus_tag="ACHA5T_000962"
     CDS             complement(11420..12202)
                     /locus_tag="ACHA5T_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM8886557.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(12195..13112)
                     /locus_tag="ACHA5T_000963"
     CDS             complement(12195..13112)
                     /locus_tag="ACHA5T_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8886558.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(13115..13399)
                     /locus_tag="ACHA5T_000964"
     CDS             complement(13115..13399)
                     /locus_tag="ACHA5T_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886559.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            14187..17414
                     /gene="fss3"
                     /locus_tag="ACHA5T_000965"
     CDS             14187..17414
                     /gene="fss3"
                     /locus_tag="ACHA5T_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFM8886560.1"
                     /translation="MYKKQKKKLCVLTILSLAVQSIVGAIAPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCVEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIADYQKKPSFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENTANIDYRVN
                     GNQLIISPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTVKIDVE
                     MEGSLKIKKVDKESGDIIPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTATIKAGETISVTSKNTREKGQIILDKTGIETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGHETQGKAEFKRAEYTL
                     FTVKDGKAVKWTENFKPELLKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDSEKNAVITRDVAAKEQVIRFGFDFFKFAGSTTGTAETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDYTKSDYIFTITEKGQKQPIKTVTVPYGNLTNENFSVSLNR
                     LMLYDLPEEEDSLTSLATWKEGDKELTTLDSTELVDKLSYNLHEIKEDWYIVAQAIDV
                     DATKAAQEKDKKAKPVAIAETTATLANKEKTGTWEILHKLTVEQVLDKTIVLFNYVYE
                     SKEAYEAGDEPVAKDASLNNQAQTVNCMVERHVSIQTKAHLEEGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALFPDGTSKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYDKDGNINGKHNEDLKEKSQTLTPKKVPTTPSTPEQSEMPTVP
                     SGSQESSPTVKAFPQTGEKTSNVLLFIGFSLIFATAGYYFWNRRN"
     gene            17526..17840
                     /locus_tag="ACHA5T_000966"
     CDS             17526..17840
                     /locus_tag="ACHA5T_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM8886561.1"
                     /translation="MELKFVVPDMVETFGKLTYGGEGEVLSEGYGRNTTIVGRSYHLF
                     SNKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLIAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            17853..18227
                     /locus_tag="ACHA5T_000967"
     CDS             17853..18227
                     /locus_tag="ACHA5T_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM8886562.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQLNQGKPTNQNEGKK"
     gene            18228..18581
                     /locus_tag="ACHA5T_000968"
     CDS             18228..18581
                     /locus_tag="ACHA5T_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFM8886563.1"
                     /translation="MKNMEINVEHILDCLDQFGKGELTEEQLTNALTYDEKMFLIMHQ
                     GLLEVGNDAKEDFDVLDWLGEEESFFMVVEVNESLCRQAESVLEEIDVEMPDAIERFL
                     KQLVETKQLPVVVND"
     gene            18650..19666
                     /locus_tag="ACHA5T_000969"
     CDS             18650..19666
                     /locus_tag="ACHA5T_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFM8886564.1"
                     /translation="MYKGHRIRAGDQHLIYHFVLGWLIFLFIGWMSVFYLQELRQFDI
                     SKVSFSTSEIGWSMKDVICLLGSLALSGSMMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQGEGFFKDLNSSWTKESISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LENKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIEEIVAENGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIQALLKSDAELFILDPKNADLADLGTIMPHVYSQK
                     EEISECVEDFYERMIARSRSMKEMPNYKTGENYAYLGLPPNFLIFDEYVAYMGANRFP
                     TSIE"
     gene            20260..22134
                     /gene="ltrA"
                     /locus_tag="ACHA5T_000970"
     CDS             20260..22134
                     /gene="ltrA"
                     /locus_tag="ACHA5T_000970"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFM8886565.1"
                     /translation="MNVETDEVQGSGKNVGKSVMEVPRLNTKIRYWEYYDLQETFDQL
                     FTQSRNGKKFYQLYELIISENNILLAYRTIKANKGSSTPGTDSFTIDNYKEMNQAEFI
                     HLILSHLENYKPKSIKRVMIPKPNGEKRPLGIPCMIDRMIQQMFKQILEPICEAKFYE
                     HSYGFRPLRSAKHALGRIMYLINISKMHYAVDIDIKGFFDNVNHRLLIKQLWNIGICD
                     KQVLAILSKSLKSPIQGEGIPSKGTIQGGIISPLLSNVVLNDLDHWVSKQWHTFETKY
                     PYTKGYNKFRALRDTNLKQGYIVRYADDFKIMTNDYPTALKWFHAVKLYLKDRLKLDI
                     SNEKSKIVNLRKCKSEFLGFAICVKQKGKKWVCNSRISNKKKDQIKEEIKQRIKDIQK
                     SPTAQNALLFNALVLGVHNYFRYATHINLDLNRMNYVLSFTTYNRLSNCSTRCYPNKA
                     SPTYKKFYSTTMKTYKVAGVYLYPLCDVSTKTIYGFNTEDTPFTPFGRQRLEHKSLQS
                     LVFQELRKLMESKIPNRTVEYLDSRISRYSMKSGKCEITKQFLPAKAVHCHHYLPKSL
                     GGDDKFDNLRIIHKDIHLLIHTTNKMIIDHYVHELKLVPEQIAKINLYRKMCNLQNIQ
                     "
     gene            22236..22616
                     /locus_tag="ACHA5T_000971"
                     /pseudo
     CDS             22236..22616
                     /locus_tag="ACHA5T_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            22661..23803
                     /gene="dcm"
                     /locus_tag="ACHA5T_000972"
     CDS             22661..23803
                     /gene="dcm"
                     /locus_tag="ACHA5T_000972"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HFM8886566.1"
                     /translation="MTFLDLFAGIGGFRLGMEQAGHQCIGFCEIDEFARRSYKAIHDT
                     RKEVEMHDITSVSDEFVRSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     ATLLQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQSRERI
                     FLIGHSRDACTEQVFPIIGSSSTSDQNIRNLVNINPSNRGMGGQVYGSDSVAPTLTGD
                     EGIKIALPVKDGISVAGMLSGNFEQGNRIYEVTGTAPTLSTKQGGTKIMIRKNETEYQ
                     EVKPGDSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKGVKAYDL"
     gene            23793..24082
                     /locus_tag="ACHA5T_000973"
                     /pseudo
     CDS             23793..24082
                     /locus_tag="ACHA5T_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24338..25522
                     /gene="mobT"
                     /locus_tag="ACHA5T_000974"
     CDS             24338..25522
                     /gene="mobT"
                     /locus_tag="ACHA5T_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFM8886567.1"
                     /translation="MAQRNLDYRLLKERRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     IASTKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDVRKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYYAIQDLL
                     KGRSIESTTFSIINRYLRFADKVDGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     IV"
     gene            25519..25632
                     /locus_tag="ACHA5T_000975"
     CDS             25519..25632
                     /locus_tag="ACHA5T_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFM8886568.1"
                     /translation="MKRGILMEIVKDMLLVLGGSMLGVTVMCLMNASAAAD"
BASE COUNT         8057 a         4994 c         5032 g         7597 t
ORIGIN      
        1 tctttgcgat acatcatacc tcttttatca attggaacaa ttatttattt ctaacaaaat
       61 cacttcattt ttgtcagata aataaaaaac aaaaaaagtt ccatctttaa aacacgctat
      121 tcttttactt tttctcacat tctctaagaa aagaaaagga gaatgaattt ttgacaattc
      181 gagcaaaagt gattatcaaa tatgtaaaat cagctacttt tttcttaaac gtttctttat
      241 actaaatata aataagtgtt tcatgaaagg agaactagat gaataattgg cttgagtatt
      301 ttcctgaaaa cgttttggaa cgcggttaca gctatcatct gcacggtttt gtccgtcatt
      361 tgaactatac aagcaagtac ttgtccgcaa cagtttctgg cacggaagat tataaggtag
      421 tgatcacttg ggatgaaaag accaacatga cttgtgattg tctatatgcc atagaaggaa
      481 aaaaatgcaa acacatggct gctgtcttat tcgcttacga agaacgtcca ataaaaaaat
      541 ccaactactc actttccgaa ctctcgtccc ttgtttcctc cgctagttcc tctcttgtac
      601 gggagctttt aacagagatt cttatagaac atccgcactt tatcgaacgc ttcaaagtta
      661 agatgccatt tcatgcaata aattattcag ataaattaac aactatcatc cataaatacg
      721 atcatattat caagaaaaac aaaaacagga aaacagcgaa gttcatcatg gagatgcgaa
      781 agttcatcca agaagcagtt gaatcgctta ttcagcaaaa tgcctatctt cccgcattcg
      841 aattgatcaa tgaagtgatt gctactctcg aaacattcta ttgggagcct gaggatgaac
      901 gaacccttct tctgatagag gactgttatt atttatggaa agaattatta gcagaagcac
      961 ctcatgcaga aaaaaggcaa atgttctctt ggttcgtctg ccaagtcgat catactgacg
     1021 cctcttattc aaaacgctac tctataaaaa tcctgaaaga agactttcgg gaaaaagaat
     1081 tttctaacca aaagaaaaaa atagataaaa aactaaagaa acggtaaaga aagacgattt
     1141 caatgaaaaa tagcaagtaa acgataacaa ccatcctaaa tccacaagaa atacagcaga
     1201 ccttgcttta aaaacacgaa tcatgcaaag gatgaagtaa agaagactta acgcgtaccc
     1261 aatattagtc atttgactat taggtacgcg ttttgtttta taagtcatga caaatttcct
     1321 tcgttgctgg atagattttc cggcattgtt gatagatttt atgatacttt tctacatttt
     1381 gtaagagagg ttccactgca tctgtatatt ggataaaatt ctgacaacaa ctttcaggtg
     1441 tgtcgaacca ccctaaccct agtgcagcaa tcattgttgc gcctaatgca ggtccttcct
     1501 ccacacttaa acaatagacc gttgcatcga aaatatcagc ttggatttgt agccatgcaa
     1561 gatttttcgc gcctcctccc accgaaacaa tacgtgtaaa tctgcgaccc gctagttctt
     1621 tacgtttcaa tcccaccatt gacaaatgct cattaaaaat ttagaatatt cacaagttta
     1681 acgacaatta tgaaaattta tgtcgtgcta aaggagtttt gttattggat gaaaagttag
     1741 atataaaaac tgcgatcaaa gacacgttac ctaccgtttt cggttatatc ggtattggac
     1801 ttgcatttgg tatcgttggg aaagctgccg gatttcatcc actagtcgtc acgttgatgt
     1861 ccctgctggt ctatgctggt tctgcccaat ttatcacagt cagcatgctt gctagtcaca
     1921 gcccattgct ttccatcgtt ttctcgacct ttctagtcaa ttcccgaatg attctgatga
     1981 gtatgacgat tgctccttat ttcaagaaaa atcggctgct tcaaaatctg ctgattggca
     2041 cattgctgac agatgaaagt ttcgcactgg gaatgaataa attgaactat actggacaaa
     2101 agttaaactt tcgttggatg aatacagcta attggatttc ttatttgacg tgggtgggtt
     2161 cttctcttgt tggcgcactt ttagggaatt ttattactga tccgaaaaag tttggtttgg
     2221 atttcgccat cgtcgctatg tttatcgggt tactttatct tcaagtcatt tctgatagaa
     2281 acacctctaa acgattacag ttgatcctga ttggtctcac tttgattctg gtatatgtcg
     2341 gactgatttt tatcccaagc aacttagtga ttgtagttgt aacactgatt gggtgcggat
     2401 tgggggtgtg gatcaaacat gccttctttt gagtttacat tgctgactat tttaggttgt
     2461 actttagcaa cttggctttc tagggtccta ccatttgtct tactgaaaaa attcgattta
     2521 ccgcaaccgt tgcttgagta tttaagtttt gtcccgatcg tcattatgtc tgctttatgg
     2581 ttcagcagtc tatttaccca aaatatcgga catctgccgc agatcaatct cgaaaatgct
     2641 ttggcttcag ttcctacact tttagctgct attctttcaa aaagcttatt ggtgatcgtc
     2701 ttggcaggaa ttctttccct ttcgttgatt cgcttattat aaataaaaaa acaagagctt
     2761 atggcaaatt actgtcatgc gctcttgttt ttatcaaagt caaataagaa aaaaatcggc
     2821 gtattcttgc ttatccgttg aacggaccac taacaacgac ttttcctaac atatgtcctg
     2881 tttctacgtc tttggttgct tgtttcaagt tcttcgcatt catcccatct gaatagacct
     2941 tgctcaatgt actgcggata atcccttcat ttgctaaagc agcaatgtgc gctaaagctt
     3001 ctccttgcgt ttcgatttca tagttatagt ctgttttagc gaacatatac tcccagtcga
     3061 agctgataga ttggtttttc caaacaccta tatctaacgg ctctttgatc cctacgatcg
     3121 ttcccacatg tccaaatggt cggacaaggt gcttgatttg atcaaagtag cgggtaatat
     3181 cgaataatac agcgacatac tctacttgat cgattcctaa ttcacgaaaa cttttactta
     3241 agtcgtgatg ataatcgatt ggatagtcta cacctgtttt tttcagccat tcaaaattct
     3301 tcggacttgc agtcgcgtag acttctaggc cagcccaatg cgctagttga ttcaaaatcg
     3361 aaccgacgcc cccggatccg ttgatcacta agattttttt cccgcgattg gcattttctt
     3421 ctggagttag accaaatttt tcaaataaca attcataagc ggtcaatgag gtcaacggca
     3481 aggctgcagc ttcttcatct gaaagatttt ttggtgcaag agcgacaata cgttcgtcaa
     3541 ctagttggta ttcttgattg cttcctgctc ttgttgttgt acctgcgtaa aaaacgcgat
     3601 cgccgacaga aaatttttgc acttcgtcac ctacagcaac aactttcccg actccatcaa
     3661 aacctaagat acgtaaagca tttcttatcc cgttatcttg gcgaagtttc gtatcgacag
     3721 gattaacaga tactgcgttg acttttacca gaagatcccg tgaactgggt acaggtatct
     3781 gttctttttc atcaataaag ctttttggat catcgatcgg caacccttca taaaaaccga
     3841 ctaccttcat taattccttt tctgacatac gaattcctcc ttagcttcat caagttggag
     3901 catccataca gtgttcattt caatatatat agtatgctat ttgttccacc ttttttattc
     3961 atcataatta ttctcttact taaagtaaac tatttgcact tttttggtta aaaaagaggt
     4021 tgcgacaaga cttttgtcac aacctcttat aaacggtgtt ctcctaccaa ctcttcggtc
     4081 acaagtcaaa atattcatca aacatgagaa gctgtttttt gaaattgctc cttgtgactc
     4141 agagttaaac ggcaggctca caacctctga tataaacggt gttcaatcaa ctgaccctcg
     4201 gtattagctc aaaaaacgac aaaatatgaa gagctatttt tgtttggctg ttcttattac
     4261 ttgatacagg acagcttttt cacgacctct aattcacggt gttcaagcaa gaaatcattg
     4321 ttcactgaaa agcgttggat gttctttttc aaaggtctct aagatctctc gtacttcatc
     4381 agtagatttc gtattcatca actgaactcg caagtcattt gctcctggaa atcctttgat
     4441 atagatttta aagaaacgat gtagtcctgt gatggaacgc gggatgattt ctgaataatg
     4501 atcttgcaga tctagttgca gacgaagtag tccgatcagt tctttttctg tatgttccct
     4561 cgggtctttt tcaaatgcat aaggattctt gaaaatccct cggccaatca tgatgccatc
     4621 aacgccatat ttctcggcca attcttctcc tgtctggcga tcggggatat ctccattgat
     4681 cgtgattagt gtttgtggag cgatttggtc acgtaaagca atgattttag gaatgacttc
     4741 ccaatgtgca tctactttac tcatctcttt acgcgtacgc aaatggacag aaagattggc
     4801 tatatcttga tgaagtaggt gactgatcca ttcttccatc tcagacattt ccgtatagcc
     4861 gatacgcgtc ttgacgctga caggcaaacc gccagcttta gctgcttcaa tcaattgtgc
     4921 cgctacttct ggacgaagga tcaagccact gccttttcca cgttctgcta cattagggac
     4981 tgggcagccc atgttgatat cgattccttg gaagcccatt tctgccatag caatgctcat
     5041 ttctctaaaa aattctggct tgtctcccca aatatgggca accatcggct gttcatcttc
     5101 tgtaaatgtt aatcttcctc gtacactgtc ttttccatct ggatgacaga aactgtctga
     5161 gtttgtaaac tcagtaaaga agacatccgg tgctgctgca tgctttacga catggcgaaa
     5221 tacaacatct gttacatctt ccattggtgc taaaacaaaa aaaggctttg gcagctcttt
     5281 ccaaaaatta gtagtcattt atttgttact ctccattcta tcaagcagtg aagtcgcaat
     5341 ctttctcaaa aacattgtca ttatactagg aaacaaaaaa agaagcaaag tgaacagctt
     5401 aaaagatgtt tctgattgag caaaaaaaag actgtgacaa aaatcttatc acagtccatt
     5461 ttccggaata aatggtgttc aaaagcttgc atctgcggta ataagtccaa ccaaacgaaa
     5521 aataggagga gctatttttg tttggctgtt cttattactt gatgcagaac agctttttca
     5581 caacctctcc actaaacggt gttcaatcag ctgaccctcg gtattcgctc aaaaaacaac
     5641 aaaatatgag gaactatttt cccgtttgtt tctctaatac tcaggtcaaa acgctgattt
     5701 cacaacctct tttctaactt ctcaatcctc ttcctttatt gatcaaatac caagtaacgg
     5761 cgtacaaaac aacgatgaat aacacaagta cgatcatcga tgttgctact ggcacatcga
     5821 tcgttccaag gaatccgtaa cggaaaccgg agatcatata aacgatcgga ttgacttttg
     5881 aaacagcttg ccaaaagggc ggtaacatag agatggcata gaaaacgcca cctagataag
     5941 ttaacggctg caagacaaat gtaggcacga tcgatacatc atcataagac tgagcaaaaa
     6001 tcccgttgag cagtcctgct aatgaaaaca agattgccgt catcaataat gtaatgatga
     6061 ccatgaacca tgaatacaca tgaagtggta caaagaacaa ggaaatgatc gttacgagag
     6121 agccgacgag aatactgcga cccacaccac caatcacaaa gccccaaata atcacatgcg
     6181 taggaacagg tgctaccaag atttcttcga tatttttctg aaatttttga gagaagaagg
     6241 acgaagaaac attggcatac gaactagtga tagcagacat cataatcaaa ccaggaacga
     6301 tgaattccat atatgaaaaa ccacccatgt caccgatccg cccaccgatc atttttccga
     6361 aaataacaaa atacaaggat gtcgtgatca ctggcggaac cagtgtttgg acccatatac
     6421 gcaaatagcg gttcgtttcc ttgatagcta aactttttaa tgcagtaaaa taaagattaa
     6481 acatttttct cctccgtatg attattttct tctgtgattt tcaagaacag ttcttctaaa
     6541 cgattcgact tattcctcat agaaagaact ttgattcctt gtcttgtaag ctgatcgaag
     6601 attccgttca ccccttgatt tcgttcaact tcgacagcga ttgtctgctc atcttcaaat
     6661 gtacaagcat atccttcaat cacaggtttg gtatcatatg gcgctaaatc aaagataaat
     6721 gtttcaaatt gcagttttga cagaagctct ttcatacttg tattctcgat cagttcaccc
     6781 gattggatga ttccgatgtt gcgacaaagc atttcagctt cttctagata atgcgtcgtc
     6841 aaaatgatcg ttgtaccatt ttcgttcaat tctcgtagaa actcccacat ctcgcgtctc
     6901 aactcgatgt caactcctgc tgttggttca tccaagatca gcaaacgtgg ttcatgcatc
     6961 aatgccctag caatcatcag tcttcgcttc attcccccgg aaagcatacg tgcacggaca
     7021 tttctctttt cccaaagatt ggactgcttc aaatattttt cgctgcgttt catcgcttct
     7081 ttgcgagaga caccgtagta gcctgcttga ttcacgacga tttgctggac cgtttcaaaa
     7141 gggttaaaat tgaactcttg tggaaccaat ccaatctgtt gctttgctcg aactagatcc
     7201 ttgtccagat cataaccgaa gacattcact tttccagatg ttttattgac taaagaagta
     7261 ataatcccga tcgttgtaga cttcccagct ccatttggcc ctaatagcgc atagaaatct
     7321 ccttctttca ccgaaagatc gattccccgc aatgcttcga ccccacttcc atagatcttt
     7381 ttcaaattat ttatttcaag cgcatttgcc atttatttcc tatctcctta tagacatgat
     7441 tcttaattgt atcatggaat acagcagatg aaaaaacaac ttggcttcca cacaaaaaca
     7501 cacctgctaa caaagaggag atgtgttttc tagttcatga tcatcttcga tagaataact
     7561 atctaaacaa ctctggaact tttaaaatca taaaagtacc gacagcaact actacgatga
     7621 catatagaag atccttataa ctgaggttct tgtctttttt attcatgtta agctttcctc
     7681 cgtcctttta tcgaaagctt ttttctttta ttttaaccga aataaataaa atggaaatag
     7741 ttattttcaa tttctgtttt cgctgtttgt tttacggaga actttgccat ggaacgtgcg
     7801 tctcgtcgct gatgctaagt tttttcgata aaagctgcta gaaggaagac agcgtggttt
     7861 attttgtatc gtcttttgct aaatcttcca agatcatctg gacagcagac tcgttcccat
     7921 tgaattctgc cacttttttg gcatcaatgt acttcttttg ataatcgtct ttttggaccc
     7981 acgcatcatg cttcgtttga taaagttaat caaacggata ccactgacgc tgttcatcga
     8041 acaacaaact ttctttttgg gcctgttcaa ctatggattt aggataattc aaaactttca
     8101 gcaaggcaat cgcatttcgg gtccttgaag gtccttcttt taatttgaaa tcaaaactga
     8161 ttccttttcc ttctgttact tgttcttcaa agtggacatt ctcacaataa ttctttaaaa
     8221 tctctgtcaa ttcgatatca tgtgtggcga caaatgctaa agatggatac tccgcaagcc
     8281 accgaacgac tgaagcggaa gaagcaatcc gttcgatcgt atttgttcct tttaaaatct
     8341 catctacgaa gcagtagcag cgttctttgg aagccacttg gtccagcaaa cgtttgatcg
     8401 atttgatttc tgaaacaaaa taactgtcgc cttcaaataa atcatcttcg attgccatgg
     8461 aagtcaacac atgtccaggt tgcagggtaa actgttctgc aatcgctgtt tggatcgtct
     8521 gtgctagaat acagctgatg gctatacttt tgacatacgt agattttcct gatgcattgg
     8581 aacccgtgac taatgtattc ttctgccagt tgactggatt aactaccgct tcttttaaca
     8641 atggatgata gatatctttc gctgtcacac cgccctcagt aaactctggt cggcaagtga
     8701 tcggcatata ggtgcgataa ttcaaaacgg ctgctgcaac ttccaattct cccagcaatt
     8761 cccacagctt gatggcttca gttgaatgct tttctaattt agtgagaacg aaatgatagg
     8821 agataaatgg cagcatgaac atgatgttga catattcaaa aatcaattct gcttcactcc
     8881 cgctcttcat tcgaaatgaa aaacctaatt tagggatgtc tcttaatggt ttagtttgct
     8941 gtttcagctc tttttgaagt ggtgtcgtca tttttgctaa ctgattggcg gctgaaatcg
     9001 ttgaaaccaa ataactcatg ctattcagct ctgtttctag tgtttgtttc ttcactagat
     9061 aatagaccgt attgaatact aagccaccga ttgctagata gattcctagt acttgcccta
     9121 aaagaagcaa acctacaccc accagcggaa gcaagccaag aaaaataaat aaacctacgc
     9181 gtccaatctt tttacttttt ccatccgcta gatattgttt tgcaaaatta tgatcttttt
     9241 ttcctaaacg agcgaattga tattggattc tttctctcag ttgaggattt tcctgataaa
     9301 aagcaattag tttttctaac tgataatctt ctccaaaatc aaagctacgt aaacgctgat
     9361 acaacgcttc tgaacctaca ctagaatagg tactgttgat cccttcaaat acttcgaaca
     9421 tatccaagtc ataccaagta agatcgtcga tttcactatc ccatgaacgg aattttttct
     9481 ccgtgagcca cgcatccttc aaactttctt ctttatcaaa tctcggttga taaggaattt
     9541 ttccccaatt actacgaact gtttccttta ctttcaaacg tgaaaaaatg tctaaaacaa
     9601 ctaataaaat aacaactgca ataataatta aaacgataaa ttctctttgg cccatatccc
     9661 tctccgttcc ttgatttgtt agttttagta tagcactgtt gcgctatttg aaaagggggt
     9721 acaaaaataa ctgtcttcgt tacctaattc gtatcaatca caccaaaata aaaacacctt
     9781 ccaaaaaaga agatgtcttt aaaacgagat acaatttttt gtcattcact tcaggtaaat
     9841 tagagccaat taaaccctcc gcaacttgac ggagatggag aagatgcccc cgtctaatct
     9901 agcggtcatc tctcctccca ttccttccac caattttttt gacaggtaaa gcccgattcc
     9961 gctagattcc gtatttgtgc ggctggtatt ttcggtataa aaacgctgga agactttttc
    10021 aacagaggtt tgcgctcctg gtttgatagg attggccact gttaatcgaa tgaatgaatc
    10081 ctcttttgaa aatgtcacct ttgcttgtcc atctgcataa cgcaataaat tctgtatgat
    10141 attttgcagc actcgcagca acatgagcgg atctgctaat acagttgtat ttatcgagtg
    10201 ttcagcgaac gctacctcga tatttttctc agaaaattgt tcataaaaag agaaaaatac
    10261 ttcttccacc tctttagaaa aagaaagcgt atggagatcc atttcttttt gcttcgtctc
    10321 tattcgagca agatcataaa actgatccgt catactgagt aatcttgcaa cagattgttt
    10381 gatgatcgtt aaatacttct cccgtttctc cgtatcctct acctgttcca gtaattgtac
    10441 ataaccgtta atagcagtca aaggagttct gagatcatga gaaaggccga cgatcgatag
    10501 tctgatgttt tcttccattt cttgaaatgc ttgtcctttt tgttcatttt catccaccca
    10561 tgtattcagt gcatctacta ggtccattag attcttttct cgaaattcga ctgaaagacg
    10621 accaccatat tcttgtcgtt cagaaagagt ggcgattttc ttagttaatc tcttgatttc
    10681 cctttgcagg aagaaaataa aacttcccat aaagataaca acgagaagca acagccactt
    10741 aatcaaatct aaaccccact ccccacaccg tatgaatata cacagcatgt gcctttgcta
    10801 gtttccctcg aagattggaa atatgcatat tcactgtttt ctcactatca aaatatggtt
    10861 cttgccaaac tgtttcataa agatttgctc gcgaaaatac ttttttcgga tgttctaaaa
    10921 aaagcttcag cagatcgaat tctcttcctg tcaaatggac agccacacca ttcacattta
    10981 catccctcga ctctttgtcc agtaagattt ccttgtaata gatgcgttgc gacagatctt
    11041 ttacttggac agaagtctga tgacgaagtt ggatgccgat tcgtaacaac agttcttcga
    11101 tatcaaatgg tttcgtcaca tagtcatccg ctcctgctgc caacaaggaa actttatctc
    11161 tttgatcgct ttttgctgaa agaacgatga tcggaacatt cgatgtctgt cggatttctg
    11221 ctaacaatgc ttcacctgaa agccctggaa gcatgagatc tagaaggatc aaatcgacag
    11281 gatatgtgct taacaataat tttccttccg tacctgaata agcatcaaga agtttgtatc
    11341 tctgttcaag aacctcctcc agtaaagaat gaacagcttc atcgtcttcg ataatcaaaa
    11401 tagtttccag caggaacacc tacttaatgt cagatttttg gaaaataaac attcctagag
    11461 ctgatgatac cacgattgtt actagactga ccagaatcat tttcggtgct tgcgaataag
    11521 ttgcttgttg cgcaaagtcc gtatacatga gccacgtttc cgtaaaccac tccgttagaa
    11581 attccaagcc tttgattttt gcgaaaacac cgggaacgaa ggccgttccc atgatataaa
    11641 gaaaagaaag aagcaaactg acactggtac ttctagtgat gaatgtcaaa gccactggaa
    11701 cagataatgt cgctaatatc agcagaagca atacaggaaa tacttttaga acataatttc
    11761 catagttttt gattccctca acttcaccaa atccgaaagc aagtgtgtaa gcaatcatgg
    11821 ctagccccgt aatcaaaacc acgccaagtg ttgtgatcaa aagcgtaacg agaagtttcg
    11881 acacataata agtcattcga ttccgtccta aagataacgt attgcgaatc gtaccgtttt
    11941 gaaattcttc cccaatataa aagcacgcaa atgctgcgaa agccagaaga atccatgcag
    12001 ccattttttc caccattaaa atccctgttg caccgttgat gactgtatcc gttgcttggt
    12061 ttaattcatt gccgtatttt gtatgcatat accattcgtt ccaagaataa aaaagcggaa
    12121 gtacgaccat cagtcctagt gagacccaca ttttacggct cttccatact tttatttttt
    12181 cagcagttaa tagattaaac atgtggattg cctccagtta attgtaagaa ataatcttcc
    12241 agtttttgcc caactaggtg gataccatcc acaggaactt ttgcctctac caatgcttga
    12301 ttgatggctg ctacttggtc aattccttca aaaagctgga taacacggct agattggaca
    12361 aaataatccc tataaccaag agagtcgaga accgttactg cttgttccgt cgccgaagtg
    12421 tcaattttta taaattgacg agattcttgt gccaattctt ctttcgataa ttctttgatc
    12481 aagtgtcctt catgcaaaat accgtagtga gtggcgatct gtgacaattc atctagcaag
    12541 tggctagaaa gcaagacggt gatgccccgt tcttttacca aacgttggat gatttctcgc
    12601 atttctgcca tccctgctgg atccatccca ttcgtcggtt catccaaaat caaaaattct
    12661 ggattcgtga tcagtgcatt tgcaatagct aaacgctggc gcatacctaa agagaaattc
    12721 tttgctttct ttttccctgt attttccaat cgcatcattt tcagcaggtc ttcgatttct
    12781 ctatccgaca caccgccgtt cgctgcttgg atacgtaggt tatctcttgc agttaattct
    12841 ggataaagag caggggtttc aatcgattga cccatacgtc gtcttgctgc ctgcaaatcc
    12901 gtcgttccaa ataattcgat cgaaccttca tcaatcgaga tcaaacccat aatagttctc
    12961 ataaaggtag acttacccgc tccattctcc ccaatcaaac catagatcat tccacgtttg
    13021 atttcgatcg atactttatc taatgctttg gcagcaccgt attttttggt tataccagtt
    13081 gctttcaaga ccgtttcatt tccaaaatcc atacttatcc ttctttctta ttgataagtg
    13141 aagtatagtt gacgggtata aagaaagcag ccagaaatgt ataaagaaag tgtaaatttt
    13201 ccaattgaat ttcaagaaaa atcagactgt ctcgcagacg tggttgagtt gtctgccaat
    13261 caaagagcgc accaaacagt gctaatccaa gtggactggc caccatggta atcgttgtta
    13321 tcgttgacat cagacgtcct tgtatatgcg ttggaacttc tcgctgaagc cagaccatat
    13381 aaaaagcaaa agaaaacatt aatccttaaa aacggactta tgaaaaataa tacatgccgt
    13441 ttgggattta caattagcta ccaaataaga ggttatgaca aaagactttt cacaatccct
    13501 tcttccgaat aaacggtgtt ctcctgccaa ctcctcggtc acaagtcaca atatttatca
    13561 aacatgagaa gcaatttttg taaattcctt cttatttctt gaagctgacc acttctgtca
    13621 caacctcaga agcggataaa taatattgtt tgatgatact ttatcttttt cccgtttgtt
    13681 tatagatcga atgatattat agaccaagaa tcctacctat taacatagct aaaaacacaa
    13741 aaaaggcact ttccaaattg tggaaagtgc cagaaaagtt aggattaagg taactgcgct
    13801 cagcattgct tcgcccagta cgattctcgt aactcgtgaa ctcgaacgat aatcgcaatt
    13861 aacgttttga gaactgtgaa gctttacggg ctttcttaag acctggtttt ttcttcgtat
    13921 cattgaataa gtttgaaaag ccttgattta taaaggtttt tccgtaaata tattttgtta
    13981 tatttgatta aatttgaaat gtttgtgttc taaaatgtgt tctaatttaa aggcagtata
    14041 tttcgtattt tatattaaat ttttaggctc attcattctc aaatccattc tatttagtca
    14101 ataaatattt taaaaagact ttataaacaa agatgttcaa gcgagcatct tatttttttg
    14161 tgcctaattt gaaaggagaa catttcatgt ataaaaaaca aaaaaagaaa ttatgtgtat
    14221 taactatttt gagtttggct gttcaatcaa ttgtaggagc aattgctcct acgattgctt
    14281 ttgctgatga aattacccat ccacaaacgg taaccgtaga actagattta gcacatcaat
    14341 atgctgttga aggaactttt agtgatggtc gtccaatgtc agaagtcact gttccgcatt
    14401 atgcagttta caatggtgtg aagcaagata ttttctgtgt tgaaccagga gtcccgattt
    14461 acaatgagtt cactcctgga tatgagaaga acccgttacc agatatgtcc gataaagcca
    14521 aattagtttc cgttttatgg aaaaaagtag gtacagatgc agatactcat attgtggcac
    14581 agaagatgat ctggcaagaa gttaatgggt atacccttca ctcaataaaa cgatcagatg
    14641 gtagtgccgt aaatatcgca gccattgagg caaaaatcaa tcaagccatt gccgattatc
    14701 agaagaaacc aagcttccat aatagtacgg ctaaaacagt attagggcaa tcgactactg
    14761 tgacggatac gaataatctg aatttgtcag agttcgatga ggttgtggaa aataccgcaa
    14821 acattgatta tcgtgtgaat ggcaatcagt tgataatcag cccaaatgcc aattcaaaag
    14881 aaagtggtgt gttgacgctt aagaaatctg ctggtaccgg aacacctgtc gcctataaaa
    14941 aggctggcca acagacttta atggccgggg cgatcgataa gccgaacact tatacggtca
    15001 aaattgatgt ggaaatggag ggttctttga agattaaaaa agttgataaa gaatcgggtg
    15061 atattatacc aggaacggtt ttccatttag actttggaaa aactttaccc gcaaaagacg
    15121 tgaccaccga taaagaaggc attgcgacct tagatgagat tcctcatggt acaaaggtaa
    15181 ccattactga aaaatcggtg ccggcgcctt atacgattga taccacaccc atgactgcca
    15241 caattaaagc aggtgaaacc atttcagtga cttcaaaaaa tacgcgagaa aaaggtcaaa
    15301 ttattctgga taaaaccgga attgaaacag ggagtgatct ttggaacgat aattactctt
    15361 tagcgggcaa tacgtttgcc attcgcaagg atagcccaac tggtgaaatc gtccaagaaa
    15421 tgaccacgga tgaaaaaggt catgcagaaa caccaaaaga gattgccaat gcgttggaat
    15481 tgggaaccta ctatgtgact gaaaccaagg ctagtcatgg ctttgtgaat actttcaaac
    15541 cagtaaaagt cgaattgaat tatgtcaatc aaacagtggc tcttgttacc agtaacgtaa
    15601 aagggcaaaa ccaagaaatt actggtgaaa ccactttgac aaaagaagac aaagataccg
    15661 gtcatgaaac tcaagggaaa gctgagttta aaagagctga atacaccttg tttactgtta
    15721 aggatggaaa agcggtcaaa tggaccgaga actttaaacc agaattgttg aagggaacga
    15781 aagcttctga tgaaaccgta actttgactt tagatgaaaa gaatcaagtt gccgttaaac
    15841 acttagcgat taatgagtat tactggcaag aaaccaaagc gcctgaagga tataccttgg
    15901 atgaaacaaa gtatcctgta tccatcaaaa aggtggatga tagcgaaaaa aatgctgtga
    15961 ttactcgaga tgttgcggca aaagaacaag tgattcgctt tggctttgat ttctttaaat
    16021 ttgctggatc aactactggc actgccgaaa ctggcttcaa tgatctaacc tttaaagtgt
    16081 cgccattgga aggaaccaat gaaatcaccg gtgccgaaga tgaagcgacc acagcttata
    16141 acgagcaatt agggtttgat ggttatggca agtttgaaaa tcttccttat ggggattatt
    16201 tacttgaaga agtagaagcc ccagaaggtt ttcaaaagat tacgccatta gaaatccgtt
    16261 ctacttttaa agaaaataaa gaggattata ccaagagtga ttatatcttt actattaccg
    16321 aaaaagggca aaagcaaccg attaaaacgg taacggtccc ttatgggaat ctaaccaatg
    16381 agaatttctc agtcagtttg aaccgtttga tgctttatga tttgcctgag gaagaagata
    16441 gcttaacttc tcttgcgact tggaaagaag gagataaaga attgactact cttgattcta
    16501 ccgagctagt cgataaattg agctataatc tacatgaaat caaagaagac tggtatatcg
    16561 tagctcaagc catcgatgta gatgctacaa aagctgccca agaaaaagat aaaaaagcca
    16621 aaccggtggc gattgcggaa acaaccgcaa ctttagcgaa taaagagaaa acaggaactt
    16681 gggagattct gcataaatta actgtcgaac aagttttaga caagaccatt gtcttattca
    16741 actatgtgta tgaaagcaaa gaagcctatg aagctggtga tgagccggta gcgaaggatg
    16801 ctagcttgaa caatcaagcg caaactgtta attgtatggt ggaacgtcat gtttccatcc
    16861 aaaccaaagc gcacttagaa gaaggttctc agacctttac tcatggtgac gtggtagata
    16921 tgtttgatga tgtatccatt acccatgatg tactagatgg ctcaaaagaa gcttttgaaa
    16981 caattctgta cgctttattt ccagatggta caagtaaaga aatttggaaa tctggcaaga
    17041 ttgattacga agtgaatgac aaagaattta ccaaaaccgt acttgcggaa aaagtggata
    17101 caagtaaata tccagaaggt acaagtttta ccttcaaaga aattaactat gacaaagatg
    17161 ggaatatcaa tggaaaacac aatgaagatt tgaaagaaaa atctcaaact ttaacgccca
    17221 aaaaagttcc gacaacacca agtacaccag aacaatctga aatgccaact gttccaagtg
    17281 gctctcaaga atctagtcct acagtgaagg cattccctca aaccggggag aaaacttcca
    17341 atgtgctact gttcattggc tttagcttga tctttgcgac ggctggctat tatttttgga
    17401 atcgtcggaa ctaaggtgat aagatgaaac atgtaaatag tagaaaacaa tacgcgcgac
    17461 cgcctcctaa aaaagtggtc gcgctatttt tgtattcaaa actaacaatg aaaagaggaa
    17521 aaaatatgga attgaaattt gttgtaccag atatggtcga gacgttcgga aaactaacat
    17581 acggtggtga aggtgaagtt ctatcggaag ggtatggtcg aaatactaca attgttggtc
    17641 gaagttacca tctgttttca aataaacaac gggccgatga tattgaagtg gtggtagctg
    17701 cggaagccgg cgaaaaggat tttgaccaag atcaaccact aaaagctgta aatccccact
    17761 taattgccaa aggctatgaa attgagaatc gtgggtttac cgattacgtg ttgtatgttg
    17821 atgatttagt gaaagcatag gaggaaaaga agatgagatt aacagaaggt attgtcgtag
    17881 attcaggatt aacctttggc aagttgcgtt tttccgcatt acgtcgagaa gtacgcaagc
    17941 aaaatgagga cggaacggtt agtaacgagg taaaagaacg gacctataat ttgaaatcct
    18001 ccgcgcaagg tcgaatgatc caagtcagta ttccagcaaa tgtaccctta cgcgagtttg
    18061 cttatgatgc agaggtagag ctggtcaatc caatcgtgga tacggtcgcc aattatgttt
    18121 ttcgggaagg aaccaccgtt aattggttta tcaaggcaga tgatttagtc ttgaaacgac
    18181 aactgaatca aggaaaacct acgaaccaaa atgaaggaaa gaaataggtg aagaatatgg
    18241 aaattaatgt tgaacatatt ttagattgtc tcgatcaatt tggcaaaggt gagctaacgg
    18301 aggaacaact cactaacgca ttaacctatg atgaaaaaat gtttctgatt atgcatcaag
    18361 ggttactgga agtaggtaac gatgccaaag aagactttga tgtactggat tggttagggg
    18421 aagaagagtc tttctttatg gttgtcgagg taaatgaaag tttatgtcgg caggcagaat
    18481 ctgttttgga agaaatcgat gtggagatgc cggatgccat cgaaagattt ttaaaacaat
    18541 tagtggaaac gaaacaactt cccgtagtag taaatgacta gcataacttt ttgaaattgc
    18601 ccaatgaaat cctattgggc tttttcgact ggaggtggaa accatcaaaa tgtacaaagg
    18661 acatcgcata cgcgcaggag atcaacactt gatttatcac tttgttcttg gctggctaat
    18721 ctttttgttc attggttgga tgagcgtctt ttatttacaa gaacttagac aatttgatat
    18781 ttctaaggta tcattttcca ctagtgaaat tggctggtct atgaaggatg taatctgctt
    18841 gctaggaagt ctcgctcttt caggaagcat gatgttactt tatatacact tttttcagga
    18901 tcattggcga agtctttggc atcgacaaaa gttagctcgg atgattctag aaaaccattg
    18961 gtatgaagta aagcagactc aaggtgaagg ttttttcaaa gatttgaata gtagttggac
    19021 caaagagagt atcagttact tccccaaaat ttattatcga atgaaagatg gcttactttc
    19081 tattcgcgtt caaatttcac tgggaaaata tcaggatcag ctattgaagt tggaaaataa
    19141 gttagaaagc ggactgtatt gtgagttagt agaaaaagaa ctcaaagact cctatgtgga
    19201 atacaccttg ttatacgata tgattgccaa tcgaattggg atagaagaaa tagtggcaga
    19261 aaatggggcg ttgcgattga tgaaaaatca agtatgggcg tatgattcct tgccgcatat
    19321 gttaattgct ggcggcacag gtggtgggaa gacctatttc ttactcacca ttattcaagc
    19381 actattgaag tcagatgcag aattgtttat cctcgatccc aaaaatgcgg acctcgccga
    19441 tttaggtacg ataatgcctc acgtatactc ccaaaaagaa gaaatttctg aatgtgtgga
    19501 agatttttac gaacgcatga tagctcgtag taggtcaatg aaagaaatgc ccaactacaa
    19561 aacaggagag aattatgcgt atctcggact tccgcccaac tttttaatct ttgatgaata
    19621 cgtggcttat atgggtgcga atcgttttcc tacaagcatc gaatagatgt gaaatggaag
    19681 cctattgaga ttatcaatag tgacaactaa tgttttgaga gatggaatga aagccttcaa
    19741 gttgagctga aacatccccg ctaatactcc tacatgctct aatcaatgaa gtagattggt
    19801 tttggtatga agctaggtga agtcgtctga aatctgccta agtgcttgag cataaggctg
    19861 actatagggc gggcggctga aaaagataac tatggtaaga atgtctgaaa atgactgacg
    19921 aatctgcgaa tgtacgggtc taaaaggctg atcttattga aagataggaa catacggacg
    19981 tatggtgaga taccgacaag taaggttcca tgatatgaaa atcgggatag ctaacgaaga
    20041 agctataatt tcacaggctc atagtagata cctaaggtta tacatgatag ctaaaaacat
    20101 tggaacgatg taagcagaaa actatcaatt aaatgactac ttctagatag gtgcatataa
    20161 gtaataacga aattgctttg tttctgtgaa agtaagggca cagtaccgat gaagcgtcta
    20221 acaaatgtgg agggatagcc ctaagtctat ttaataacaa tgaatgttga aacagatgaa
    20281 gttcaaggat ctggtaagaa cgtgggaaaa tccgttatgg aggtgccacg cttgaacaca
    20341 aaaatacgtt actgggaata ttatgatcta caagaaacat tcgatcagct atttactcaa
    20401 agtagaaacg ggaaaaagtt ttatcaatta tatgagttaa tcatttctga aaataatatt
    20461 cttttagcat accgtactat caaggcaaac aaggggtctt cgacaccagg aacggattcc
    20521 ttcacaattg ataactataa ggaaatgaac caagcagagt ttatccatct tattctcagt
    20581 catttagaaa attacaaacc aaaatccata aagcgagtaa tgatacctaa acccaatggt
    20641 gagaaacgcc cattaggaat tccatgcatg attgatcgaa tgatccaaca gatgttcaag
    20701 cagatactgg agccaatatg tgaagctaag ttttatgaac atagctacgg tttcagacca
    20761 ttgagaagtg caaagcatgc tctaggtcgc attatgtatc tcataaacat cagtaaaatg
    20821 cactatgcag tagacattga tatcaaaggt ttctttgata atgtaaacca ccgtttactg
    20881 ataaaacaat tatggaatat aggaatttgt gacaaacaag tcttagctat tctttcaaaa
    20941 tctctgaaat cacccattca aggagaaggt atcccgagca aaggaacgat ccaaggaggt
    21001 atcatttctc cactattatc taatgttgtt ctaaacgact tagaccattg ggtctcaaag
    21061 cagtggcata cctttgaaac aaagtatccc tatactaaag gttacaataa atttcgtgct
    21121 ctaagagata caaatctaaa gcaaggctat attgtcaggt acgcagatga ctttaaaatc
    21181 atgactaatg attatccgac agcattaaaa tggttccacg ctgtcaagct ttacctaaaa
    21241 gacagactta aattagatat ttccaatgag aagtctaaaa tcgtcaattt gcgtaaatgt
    21301 aagtcggaat ttttaggatt cgctatttgc gtgaaacaaa aagggaaaaa atgggtttgt
    21361 aattcacgta tttctaacaa aaaaaaggat cagattaaag aagagattaa acaaagaatc
    21421 aaagatattc aaaagagtcc tacagcacaa aatgctctcc tgtttaatgc tctcgtgctc
    21481 ggtgttcata attatttcag gtatgccact catattaatt tagatttgaa taggatgaac
    21541 tacgttcttt cattcactac ttacaaccga ttatcaaatt gtagtacaag atgttacccc
    21601 aacaaagctt ccccaactta caaaaagttt tactcaacaa cgatgaaaac gtataaagta
    21661 gctggagtct atttgtaccc tctttgcgac gtatctacaa aaacaattta cggatttaat
    21721 acggaggata caccgtttac tccgtttggt cgtcaaagat tggagcacaa aagcctacag
    21781 tctcttgtgt tccaagaatt aagaaaattg atggaatcga aaataccgaa tagaacggtt
    21841 gagtatttag atagtagaat atctcgttac tcaatgaaat caggcaagtg tgagattaca
    21901 aaacaatttt tgccagcaaa agccgttcac tgtcatcact atttgccaaa aagtcttggt
    21961 ggagacgaca aattcgataa cttgagaatt attcataagg atatccatct tctgatccat
    22021 acaacaaata aaatgataat cgatcactac gtgcatgaac tgaaactggt gccagagcaa
    22081 atagcaaaaa tcaaccttta tcgaaagatg tgtaatctgc aaaacattca atagaaaata
    22141 aatagatgga acgccgtatg aggtgaaagt ctcacgtacg gtgtggagcg ggggaaaaga
    22201 tggagattat atcaaattct tacctatcgc tatagatgtt aacgacaaaa gaaagtgcgg
    22261 tgattttgaa taagcttaaa caaatcgtga tgttaggccg tcaatctggt ttctttctga
    22321 ttctagcttg tcaaaggccg gatgcgaaat atctaggaga tgggattcgc gatcagttta
    22381 acttccgtgt ggctttgggt cgtatgagtg agcttgggta ttccatgatg tttggagaag
    22441 tcgataaaaa tttttttatg aaacgcatca aaggacgagg ttatgtagat acagggggca
    22501 gtgtgattag tgagttttat acgccacttg taccaaaagg gtatgatttc ttaagggaaa
    22561 ttagcaacat tgttggatta accgttcata cggaaagaga aaataatagt gtgtagccta
    22621 ggaatatcaa taatcctggg cttttttatt gaggtgattt atgacatttt tagatttatt
    22681 cgccgggatt ggtggctttc gcttaggtat ggaacaagcc ggtcaccagt gtatcggttt
    22741 ttgtgaaatt gatgaatttg ctcggaggag ttataaagca atccatgata caaggaagga
    22801 ggtggaaatg catgacatca caagtgtatc agacgaattt gttcgatccc tcggaccagt
    22861 ggacatcctt tgcggcggct ttccgtgcca agctttttca attgcgggaa agcggcaagg
    22921 attttccgat actcgaggta ctttattctt tgaaatcgct cggttcgcca ctcttctcca
    22981 acctaagttt ctattccttg agaacgtccg gggattgctc aatcacgaag gaggggctac
    23041 gttcgagacg atcctccgaa cgttggatgg attggggtat gatgtggaat ggcaagtgct
    23101 taactcaaag gcctacgttc cccaaagccg tgagcggatc ttccttatcg gacattctcg
    23161 agatgcatgt accgaacaag tatttcctat cattggatcg tcttcaacat ctgatcaaaa
    23221 catcagaaac ttggtaaata tcaatccttc caatcgagga atgggtggcc aagtctatgg
    23281 ttcagatagc gtagcaccaa ccttgaccgg tgatgaagga attaaaattg ccttaccggt
    23341 aaaggatggg atttctgtag cgggtatgtt gtcgggaaat tttgaacaag gcaataggat
    23401 ttatgaggta accggaacag cccccacttt gtcaacgaaa caagggggaa cgaagattat
    23461 gattcggaaa aatgaaacag aatatcaaga ggtgaagcca ggggacagtg tgaacctggc
    23521 ttttcctaat tccacaagtc gccgaggtcg tctgggaaaa caaagtgtgc ataccttact
    23581 aacaggagac caacaagcgg tagtcacgga tcagtaccag attcgaaagt taacacctag
    23641 ggagtgctgg cggcttcagg gctttccaga ttgggctttt gatcgagctt ctcaagtcaa
    23701 ttcagatagt cagttgtata aacaggccgg aaactcagta acagttccgg tcatttttga
    23761 tattgctaga agattaaagg gggtaaaagc atatgattta tgacgagttg attggagaaa
    23821 tctactggat gattggcaag attcaggcag atccgaaatt ggaagaagaa ctgtaccggc
    23881 tcaattttga tattcgcaaa aacggggtga aagtccaggt gattcgtatg tagtggatga
    23941 ggaaacggat gctcgaatgg agctcaatca agtcatcggt gaattggaac gaattgcgga
    24001 ccttgccaaa gaacctgata tccgccaata cttgtttgag ataaaagcag agttggaaat
    24061 tgagggtatc accgcggagt aatagggaaa taacccactg ataggcactg accgcggggg
    24121 atttgcgaag cagaaatcct ccgtggtgct gtgcctgttc atggacggcg gcagccggcc
    24181 atgatgtctc accccccgta tctaacaggg gggtacaaaa acacaaaaaa caagcaagta
    24241 accataaaaa atgcttgttg tatcaaggat attaagtaat ttgtataggt gttaactcat
    24301 ttggaaaagt taacaccttg aatttggagg tgaaaacttg gcgcaacgaa atttagatta
    24361 tcggttatta aaagaacgac gcaacgaata tggcgtttca caaaataaat tagctactgc
    24421 ttgcggactt agccgcccat acctaaatca gatagaaaac ggtggcgtaa tcgcctctac
    24481 aaagaccatg agaaaaattt ttgatcaact ggaaagtttc aatcccgatt tacctttaac
    24541 gttactattt gactatgtaa ggattcgctt tcccacaacg gatgtgcgga aaattattca
    24601 agaaattctc catttgaaat tcgagtatat gcttcatgaa gattacgcct tttactccta
    24661 tcaagaacaa tatgtcatgg gcgatattgt agtgatgtta tctcatgaag aagataaagg
    24721 tgtccttttg gaattaaaag gtcgtggttg ccggcagttt gaaacttttt tactcgccca
    24781 aaagcgtagc tggtacgact tttttgaaga ttgtctaaaa gccggtggcg tgatgaaacg
    24841 cttggattta gcaatcaatg atcgagtggg gctactggat attcctgatt taacgaaaaa
    24901 atgtcagaag gaagaatgta tctccttgtt tcgtaccttc aaaagttacc gttccgggga
    24961 actgttgaaa gctgatgaaa aggatggtat gggcaatacc ttatatattg gcagcctcaa
    25021 aagcgaggtt tatttttgtt tgtacgaaaa agattatgaa caatatatca agttagggat
    25081 tccattggac cagaccgaaa cgaaaaatcg ttttgagatt cggttgaaaa atgaccgtgc
    25141 ttattatgcg attcaagatt tgttaaaagg tcgtagcatt gaaagtacca cgttttccat
    25201 tatcaatcgg tatttgcgat ttgcggataa agtagatgga aaacggcgaa ccaattggcc
    25261 cttgaatgaa caatggggtc gttttattgg acggaatcgg aaagagattc aattaacttc
    25321 tgaaccgaaa ccgtatacca ttgaacgaac attaaattgg ctaggtagac aagtcgcccc
    25381 tacgtggaaa atggctaagg agttggatcg attgaatcaa acaacgtata tccaggatat
    25441 ggtacagaat gcgcgactct cggaccggca taaaaagatt ttggagcaac aaagtatggc
    25501 aatcgaaaac ttgattgtat gaaaagagga attttgatgg aaattgtaaa agatatgtta
    25561 ttggtacttg gtggcagtat gcttggtgtt accgtgatgt gcttgatgaa tgcaagtgca
    25621 gcagcggatt gagcaatgga aaagaaagga aagtgacaaa atgaatttcg gacaaaattt
//
LOCUS       DAYYGO010000028        25469 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000028 DAYYGO010000000
VERSION     DAYYGO010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.028"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..191)
                     /locus_tag="ACHA5T_000976"
     CDS             complement(<1..191)
                     /locus_tag="ACHA5T_000976"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFM8886569.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEF"
     gene            complement(337..1215)
                     /locus_tag="ACHA5T_000977"
     CDS             complement(337..1215)
                     /locus_tag="ACHA5T_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8886570.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(1254..2372)
                     /locus_tag="ACHA5T_000978"
     CDS             complement(1254..2372)
                     /locus_tag="ACHA5T_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFM8886571.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(2369..4468)
                     /locus_tag="ACHA5T_000979"
     CDS             complement(2369..4468)
                     /locus_tag="ACHA5T_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFM8886572.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(4519..5340)
                     /locus_tag="ACHA5T_000980"
     CDS             complement(4519..5340)
                     /locus_tag="ACHA5T_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8886573.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(5337..6146)
                     /locus_tag="ACHA5T_000981"
     CDS             complement(5337..6146)
                     /locus_tag="ACHA5T_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8886574.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(6167..6661)
                     /locus_tag="ACHA5T_000982"
     CDS             complement(6167..6661)
                     /locus_tag="ACHA5T_000982"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8886575.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(6673..7173)
                     /locus_tag="ACHA5T_000983"
     CDS             complement(6673..7173)
                     /locus_tag="ACHA5T_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8886576.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            complement(7238..9934)
                     /locus_tag="ACHA5T_000984"
     CDS             complement(7238..9934)
                     /locus_tag="ACHA5T_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8886577.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(10362..11435)
                     /locus_tag="ACHA5T_000985"
     CDS             complement(10362..11435)
                     /locus_tag="ACHA5T_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8886578.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(11432..12259)
                     /locus_tag="ACHA5T_000986"
     CDS             complement(11432..12259)
                     /locus_tag="ACHA5T_000986"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8886579.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(12260..13066)
                     /locus_tag="ACHA5T_000987"
     CDS             complement(12260..13066)
                     /locus_tag="ACHA5T_000987"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8886580.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(13078..14163)
                     /locus_tag="ACHA5T_000988"
     CDS             complement(13078..14163)
                     /locus_tag="ACHA5T_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8886581.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(14180..14722)
                     /locus_tag="ACHA5T_000989"
     CDS             complement(14180..14722)
                     /locus_tag="ACHA5T_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFM8886582.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            14980..15969
                     /locus_tag="ACHA5T_000990"
     CDS             14980..15969
                     /locus_tag="ACHA5T_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFM8886583.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIITFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            15966..16703
                     /locus_tag="ACHA5T_000991"
     CDS             15966..16703
                     /locus_tag="ACHA5T_000991"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFM8886584.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            16700..17251
                     /gene="coaC"
                     /locus_tag="ACHA5T_000992"
     CDS             16700..17251
                     /gene="coaC"
                     /locus_tag="ACHA5T_000992"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFM8886585.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            17267..17899
                     /locus_tag="ACHA5T_000993"
     CDS             17267..17899
                     /locus_tag="ACHA5T_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8886586.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            17984..18631
                     /locus_tag="ACHA5T_000994"
     CDS             17984..18631
                     /locus_tag="ACHA5T_000994"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFM8886587.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            18636..19619
                     /locus_tag="ACHA5T_000995"
     CDS             18636..19619
                     /locus_tag="ACHA5T_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFM8886588.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(20022..21536)
                     /locus_tag="ACHA5T_000996"
     CDS             complement(20022..21536)
                     /locus_tag="ACHA5T_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFM8886589.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            21674..22480
                     /locus_tag="ACHA5T_000997"
     CDS             21674..22480
                     /locus_tag="ACHA5T_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886590.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            complement(22655..23224)
                     /locus_tag="ACHA5T_000998"
     CDS             complement(22655..23224)
                     /locus_tag="ACHA5T_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8886591.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(23307..25366)
                     /locus_tag="ACHA5T_000999"
                     /pseudo
     CDS             complement(23307..25366)
                     /locus_tag="ACHA5T_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
BASE COUNT         7593 a         5401 c         4441 g         8034 t
ORIGIN      
        1 aaaaattcaa acattaagcc aaggattatg aagtcaacat ttcgaaaaat gaacatacat
       61 tggtaagcaa ctataactat taccacagac ataaagtaac acaatatatt actatttaag
      121 tgtacgccac cagtgaatat cctcaaggta tacagcgcac aacaaaaaga aaaaaggaaa
      181 cgcagaagca tttcctttca gtgatttaat gaaaacaaca cgatttgact tagagccttt
      241 ttatttgaat tcattttcgc tttatccaac ttcatttgat catttgatta ctcggttaaa
      301 tacttttgac atgagtatca atgaaacatt cattaattat ggttgcttat ttcttaccgc
      361 ttttctggcc aatgctaatc cgccgattat tccggcatta tcacctaatt cgggcgtaat
      421 aatgtagcta tcaattggtg gtatttcaac atatccattt ataagctcaa caaatttatc
      481 ctgaactttt ttcttcatgt gttcttgttt cattacacca ccgccaaaaa taattctatc
      541 tggcgaaaac atcagtgttg tattgtatgc acattgggca aggtaaaagg cttcaatctg
      601 ccaaaacgaa tcgtcagcca ataaattctg gccttttaca cccaaccgtt tttcaatcgc
      661 tggtcctgct gccatcccct ctaaacaatc ttggtggaag ggacagtttc ctgaaaaatt
      721 gtcctttgga tgtcgcttga tcaacatatg ccccatttcc ggatgactga acccttccac
      781 gaaagtccca ttgaacaagg caccacctcc gatacctgtt cccacagtga agtatgcaac
      841 acctgctaac cctttgccag aaccagcaac atattcacca tagcaagccg cattgacatc
      901 tgttgtccat tcaatcggaa tattgaaata ttgctttagc gttcctacaa aattgaaatt
      961 ttgccaagca agttttggtg ttgatgtgat atgaccgaaa ctcgttgatt tgcattgaat
     1021 atcaattgga ccaaagcagc cgaccccgat gcctgccaat tgttccttaa aaggtttaaa
     1081 aaaatcaatg acctgcttca ttgtttcaac tggagttgtt gtgggaaaac tgactctttc
     1141 caaaatcgtt aattcctcag taccgactgc acagacaaat ttagtccctc cagcttcaat
     1201 tgccccatat atcatcatag gttcctccct tcgaatatgt gaaaaacact tcattaacgc
     1261 ggcaagcgtt ctcgatcagt gaaatgacgt ttagtatttc ctgaagcttc tgttgaatac
     1321 cagtaagcga cactagcaac atcgtcttgc cgctcaaata agcgaacatc atcgttgccg
     1381 atttgttgta aagtgaccga taaatcttcg gtaaaacaga tggggtcttg aagatgccaa
     1441 cgataaagac catgtcgtgg cattgcgtcg tcaccaaagg catgaacggc attcaatttt
     1501 cccgtttcaa aacgatctct tgtggcatct ctatttgatt ggtaaggata ccctaaaaag
     1561 gctgttgaat agtttttcgc atgtggacgt ccgaactcat cgtactcatg aaaggcccac
     1621 gccccgccaa aataatcttc tgaacctgtt gaggttacgg tcggatactc actatcccca
     1681 tcaatgtaga atttaaactc gccttcgccc caccaataac gttctaggca ggtaagcgca
     1741 aggtaagtac ccacgtaata gccttgaccc tttaatcgat caattaactt ataatcctcc
     1801 tgtaatttgg taatgcgttc ccgatgccaa tttgcgtgaa aatataggct attttcttcc
     1861 aattcgtcca ccaaagcgta atttatcgta taaaaaaatg aggacatatc cttcggatgt
     1921 aaattctcga tcgtaatttt agcggatgtt tggaaaggca tttcgaaata ggaattaaag
     1981 ccccctgtag gattaaccac tatcggcaac gagttcacct cgcaacgctc tccaaaccca
     2041 ttacaaaaga aatcccctaa aggactttca acagcagggc tatccgaccc atcccaataa
     2101 attcttaaga ttaaatcacg taaaacaaaa ctgccaaatt ctgttcgatc acgaatcgtc
     2161 atccacatat gacgaattat tcccgttcca gtaatatccg caatcacagt ttcttcacca
     2221 gaagtcagcg gaattgcgcc acgacctttg cggcttggtc ctagctcgct tggcaacatc
     2281 gcagctttac ctttctcgcc tgttggattt tcgggagtga ttgctcgact ctggattttt
     2341 ttaaattggg taatattttt tagtatactc atctttttct cctttgaatg tgaatcgttt
     2401 ttcccttttc tacgtgaaga atttttttcg tatcacttat tttcaatggc ttttctgaca
     2461 aatttgaaat actcaactcg gattgcgtac attcaatcga aatgaaggct tgaccgaact
     2521 tcaaattctt ccaagaaaac gttaaattat caacaggatc aatttgcaat actccttttt
     2581 tgaatttcaa tccgaaatat tgactgatca ttaggctgac aaaaagaccc gatgcccagc
     2641 cgcatttgcc gcagccaaag tctcgcacga cttctccttg ttgagcctct aagcgatacg
     2701 gccaccacca ccacgaaccg tctaaatctg ccaaacaaat tagttcttct aatcgggtta
     2761 cgaaagcttg agggttgtgc agctcgctca ttaaaacagt gataaagcca ggaaaagtgg
     2821 cgccggattc taatccccaa gtgatgcctt tgatttcctc gctataggtt ggattgaaag
     2881 aactagcaga aaaggccatg gtgttctgat ataaatgatc tttattatct aaaaattgat
     2941 aaaaaggcat caacgtagtg tctgattctt ctccatcatg catcaataaa ttaatttttc
     3001 catccacaat cacatcactt aaaaagatta acccttgctc aagaatcggt ttttgatagt
     3061 gcttcactga ataggttgtt ttctcctcat caccgattcc ctccaaaaat tgctcaccaa
     3121 aaggaccatc gatagtcatt tgtttacaaa ttgattcctt tagtttacag gcaatgtttt
     3181 tgtatcgctt gctaagactc agttgttcta gggcttcaaa aatttctgca agtccaatac
     3241 aggccttcca aagacaaaga ttgctgccgg tatgatactt tccaagtgca aaggcgtctg
     3301 ataaccaaac agatggaaaa agcattggtt cccctgaccg acgttgagct aatatgtcct
     3361 caataattct tgaagcattc agtagaacct gcggacaccg ctcaaagaat tttgtatccc
     3421 cagtatgttc aaaataaaga ctagccagta acgcactgga taacgaatta ctcagagaat
     3481 gattgatacc tcctgtaaat tttgaaccag gaaacttaac gccatattga tcaaaccata
     3541 gaaccgtttt ttgacataac tccggttcaa acatggtgaa tggcaatgat gcgtaataca
     3601 tatccttatt ccaaattcga ttagtagcag gatagctgcc ccaattgctg ccgacgacct
     3661 gtccctgtcg attcataccg aaactagaaa agctttgata aagtgcacga cgatagagat
     3721 ggacaactct atcgtttggc attgacagct gcccaaatag ctgagcaaaa tagttcaatg
     3781 tttccttcag ccatttttcg atatcagatt cctgaaaagt cattacttct tcaaaagcat
     3841 ttgggtccac aaaagcaatg gcgaagcttg ccacttcctg ttgtgcgatc gtgtccttat
     3901 ttcctttttg agaaatcaac acattttgct gatccgaata cttctcttga tacgaaggca
     3961 catctttcac tgaaacttga aggctttcgt gtgtttcatt cacaatgtaa acctgttgaa
     4021 caagcatact taaacgctta ttagccagta tgggagcaaa ggatactaca atgaccttaa
     4081 acgtcgaata atgatgaata tatctaggca aacagtcttg aatcacatcg acttcaatac
     4141 gatgatccga ttctgacaaa tggtagacaa tcccgtttac ttcaaccttt aaggataacg
     4201 aattggtttg agagagctgt ttattgaccc aaacacctgg tttttcctca gttagtaaat
     4261 tttctcgata aaaggtcaac gtttgaatcg tcccgcaaac atcaaggttt gctaatgtct
     4321 tttgattact tacatcgaag gtcaatccct ttacatcagg aatattccga tagtagctgt
     4381 attcgtttga agtagtcaca tttccgtcgt ttaactcagc attaaaaaat gggtgtttgg
     4441 gtgaataaat tacttcatta atagtcatct tttccctcct tgctaaaaaa actgcagcct
     4501 agtagctgca gcttcctact atgcgattcc aagtaatgac aataaaattc ccaagacgac
     4561 cattccaaaa ataattttga tgacgccgac ttttttatct agtaatttga aacagctcaa
     4621 tgttaccaat aacggtacaa ttcctttaaa aatactatcg aggatttctt ggacattcat
     4681 tagttcttcg cccttcattg aaaagctcaa aacggtattg aatttgacca tactgatcgt
     4741 cattgcacct accatgatca gacccaagat actagcagct tttgtcagaa tttcaattaa
     4801 cccgttcgca tacagctttt caatatattt agagcctaat gaatagccta aaacgccgcc
     4861 atagtatcgc atcaagtaat taggtatatt gaacataact aaaaagataa ttggtgccaa
     4921 aatatttccg gcattggcta agttgatcgc tagtcccgcg gtaacgacac gccaaactcc
     4981 ccagaacaat gaatcgccaa ttccagccaa tggtcccatc aaactcgttt tgatcgcgtt
     5041 gatactttcc acgtcaaaac cagctttttc agaattttcc ttctccatcg atgccgcaat
     5101 ccccatgaca aaggacgcgc aaggaatggt cgtcgaaaaa tactgtgtat gacgttccag
     5161 cgccgcagct cgatcttctt cacttttgta gaaatgatta atgaagggca tcatagaata
     5221 ggcgaaacca ccgccgccca tcgtcaccgg attcaccgaa gcatacatag tcatcgagcg
     5281 ccaaaacatc ttccgaacca attttttctc atctttagaa attccatcag ctaatttcat
     5341 tgaaagaaat cctcctcttc ttggtcaagc gttgcattta cagcagcatc gctttgaact
     5401 gcatgaacag atatattttt tagttgtaga tctctttggg ctataacaat tgcaataatc
     5461 gttccaactg cggcaattgc aatcaatggt aactcgagat acgccataac aacggcccca
     5521 aagaaaaacc acacagcaat atttttactc caaagcatcc gtaacagcat cgccatccca
     5581 actgccggca gcagatttcc agcgactgtt aatccgttca tgatgacctc tggaatatta
     5641 tccaagacgc ttttgacaac atctgctcca aataaaacgg caaagaatgg aataagagca
     5701 taaagcgcat agtggacaaa ccacataaag aagtgcagat tgatgattcc tttttgatca
     5761 ccagattttg caaatttgaa aaatttagca gccatcggtc cacatacaaa agaaaaaatc
     5821 atattcatta actgtaatcc taatactgct agtggaaaga caatgggaat aatcgcatcc
     5881 acgtttcctc ccattttgat tgcgaacgct gtagccagcc cagcagccaa agaaggctcc
     5941 gccgtactag agattccgat attgacgaca cccataaaga tggcttctag tgacgccccg
     6001 acaatcaacc ccgtctgcaa atcccccatc aacgctcctg ctaacggagc aacaacaatc
     6061 ggacgactca gcatggacat cccgaacatt tcatgtccag caactccaat aaaaacggcc
     6121 aaagcgacta caaatgcatc atacataatt tttctccttc ctttttctaa aaatcactca
     6181 aaatagagat agctgaacgt ttttccatcg ccggtgtagg ctgaaaaacg gtatctggat
     6241 aaagttcaac taattctttt aagtcttgaa tttctgacgt cgttaacatc acttcctttg
     6301 ataaggtttt cttatcttcc gatggcggtg tcattttccc agcattcccc acgttcatta
     6361 attcgatttc cgttgggaaa gctttcgcaa cttttaccgc atctttgaca ttagggacta
     6421 aaaccatcaa gcgcatgtcc ttcgcccgcg gatcttttaa caaatgaatc gcgtcatcaa
     6481 ctgttttaat aatgactttg acattcgatg gtgcagccat cttcaatgtc atcttttgaa
     6541 tatcgttggt agccgcctca tcgcttgcta ctactaaccc ggttaaattt agactctttg
     6601 tccaagtcat cgctacttgt ccatgaatta atcgttcatc aattcgcact gctttaatca
     6661 tttttcttcc tcctaaaaaa aatcttcctc ttcatttact acatgtacct catcaagaat
     6721 ggtcatcttt aattcctgtt ggcatttatt taccagttcc tgaatatcct cctttgttaa
     6781 ttccttggtt gccaatacta tttcgagcag aattggcaga ttaaagccag acaaaatagt
     6841 tacagcaatc tctttctcca aaaaggtttt ggtaatcttc tgattgacac ttccgccaaa
     6901 taaatcggta aaaacaaaaa tctgatcctg ctcgttcacc tggtttacaa atacattcaa
     6961 ctcctgttca aaatcacact catccacata agcattaatc accgtaatct cttgaacctt
     7021 ccccgtcagt aattgaatac tgctaagaaa gcctgaagct aagtgaccgt gagtcgctat
     7081 tagaattttc ttcatatggt taattccttt ctttctgttc acttttatat aagcaattcc
     7141 tgtgccaact tttaaaaaca ttggtttaat cacgttaatt cacattaaaa aaagtgtcta
     7201 acctaaaaaa aacagattag acactttttt agacacttta tttttgaata tacggtccta
     7261 ataattcata gagcagcgaa agctcataag cattgacttt aaaattatat ttcatctcca
     7321 ttgattgaaa aatacttcta gaaacataat aaaattcatc ttcatctggt ttcaaatcat
     7381 aattcggttc ttcagatttt tctgtccgag ccaagaataa acgttccatc atcaatgccg
     7441 tatgcatata gagatttagt tttacacggc catttaaagt gaaatgatag taattttcat
     7501 atttagaaat gactgtttcc acttcactca tcacgatcgt tgggttcaga aagttcaaac
     7561 ggtccgcaat tccttccaac gaaaagaatt ttactagctc ctgaatcaag cggttaaatt
     7621 ctgccggctt catttcagga ctcaaaatat gctccaaatt ggctgctcca tcaccgtcta
     7681 agatgtcata aacattaata ttaggaattg aaaaggaacg tggaagatcc gaagtcgtga
     7741 tgaccaattt tgtcttgtca aaataagttt catcattttg gtcgattaat tcacgtagtt
     7801 ctctgtagtc cttcgtgaaa acttcgatgg attcatccga taaatgatga cgaaaaacat
     7861 ctttcaattt gtccgaaatc cccaaacccg acatacagga aatgataatg ttgctacttt
     7921 gagaaaagcc ttcaaagtat tgaacattga ttgaatactt gtctgctgca tcctccgcaa
     7981 tctgtttaaa tggcgcattc atcgtcatct tcaagccaat atcaagtgca atggaagtcg
     8041 tcaaattatt cagaagaagt aactcccctt ttaaatcgtt cttgatctgt gtgtaaattt
     8101 gatttaaaga gcccatatct acgatcagca ctagactatc tgtattcact tgccgtttca
     8161 cgaaattcct tgtcgattcg acaatctctg acacattact ctctactggc atatccaacg
     8221 cttcaaaaac ataattgccg cataattgat tcactactgc ttgaatgctg ctagccgtac
     8281 tatctccgtg agcaagcaac aggcccttca gctcgatgga ttcatctaca taatcagata
     8341 aagtcacgac aataagcagt cttagaaagt tctcgtcctt ttctgagagc ggtggtaaat
     8401 gctccaagaa ctgattacat aagtaagtcg tccgaggtag atttgcagct aatgtacgat
     8461 ttatcccggt taagtctgtc ttatagcttg gggaaatgac ataggatctg ctaataattt
     8521 ctgcaacagg tccactcgtc gtcaatccgt acgtttttcc taaaacggat acccagacct
     8581 ttctaaacat ctgaatcatc aattttctac tcattgataa aaaagaatcc ttatcaatgt
     8641 gatgcaccaa atgctgtact tcattcttta tcgaagaaaa attttcattg aaattacctt
     8701 catttatcag ctgagtaatt ttttccaaaa tcgaatcata atcaaacggt ttgattcgat
     8761 ccgtttttat cttttgattt ttctttatca ataaactagc aatcggcaag ctttctactt
     8821 cggattccag catatttaac tgaatcaaga tctcattttt cccaatcgtt aatggttctt
     8881 cttgtgttcg actatacaca tccgcacaac tgattttaat gatattcttc agcttaccaa
     8941 tattcccact aaagggatga gataacatta aagacaaggc ttctcttgaa acaataatat
     9001 ctttttgtaa aacatccgct tcattttgaa aaaataattg aatcagctct aatcgttctc
     9061 gaatggggcg atcttcaaaa gttggcaaca taacactaat ttgaatacgc cgatcaaagg
     9121 tgtctaataa ataattttca cctttttccg tcgtggcgaa aataaaacga accttagcag
     9181 aatgccaatt tacattttca ccgactggac gatagtttcc agtatcaata aatagaaaga
     9241 gcttttcttg attttctttc gataggcgat ggacctcatc gagaaaaaga tagccgttgt
     9301 tggccgttgc caataaacca tcggtatcct tttcagcccc cgtaaaggcc cccttcttgt
     9361 atccaaacag tgttgccgaa agcagttcgg gattgtccgc ataatcggca cagtttaaga
     9421 tagcaaatgg tgcatctttt tcaataattt gctcatggat agcgtattca aaaatttttg
     9481 tggctaaaaa actttttcca acacccgtgg ctccatttat taatacactg agtccgttag
     9541 gcggatattt gacagcggct atacattttt gaatgacctc tctcagactt ccgtcatgac
     9601 caactacaga atgaaaggct tcgtttattt gctccgtatt atctacaacc gcatattcat
     9661 ctaaaggctt ccacaaaaca ggctttcccg gtaatttctc gaccttttta tcttccacta
     9721 atctcgtcaa ataatggctg gcattctgac gagagatacc tagattcttc gaaattttct
     9781 cggcagtgac agaagacata ttccctgtta gtcgtatcag ctcatcaaaa actcgatctt
     9841 tagcgctctt tttcattttt tcctccagtt taatgtgtct aaaagcataa taaactatcg
     9901 gctgcaatat gtctattcta tatttgtatt ccaattttta ctttgaaaca caaatagtcg
     9961 aatatttgcc tcagatatct gacaaaataa tttatctacc aatttagttt ttatctaata
    10021 tgatttttat gttttttcat agctacaaat attgaactgg attcatatat tagttttatt
    10081 aatagattac agaagggtta agtatatttg catagtttcc aattacttta tccttctgta
    10141 actttttcaa tcttagattt actagcccgt ctcgggccct attttacaat ggttttgtcc
    10201 acttttctct ggtagaaaaa cgtggtagaa aaaataaaat aacacaaaaa aagcggcgtt
    10261 tcatccgctt ttggtagaag attggaaaac tcagatagaa aaaggacgat ttttagctct
    10321 ttagaacgtt gataagtccc cattcttact gagttttgcc attatctcct atacatcttg
    10381 aattctaaga ataggtcatt atagatgcct aggtatttcg atcctagatc tttgtagact
    10441 tcaagatggg agatcgtgtc atcagatgga tagaattgtt cgtccgacga aatgtctttt
    10501 ggcaataacg cttttgcttc ttcatttggt gtagaataac caatatactc tgcattttgt
    10561 gccgcatttt ccggttctag cataaagttg atgaaatcat aggcaccttc tttatttttc
    10621 acggtttttg gcataacgat attatcaaac caaagattgg atccttctgg tgggatcaca
    10681 taatgtaaat gttcattttc ccacatcata tcggcagctt cgccagaaaa tgtcactgcc
    10741 acggaggctt cttcattgat catatacatt ttgatctcat ctgccacgat tgctttgaca
    10801 tttggtgtta aatgattcaa tttatttgct gcttctgtca gctgtgccat attcttacta
    10861 ttcaaagaat agcctaaact attcaaagaa agccccataa cttctcgtgc accgtcaatc
    10921 agcatcaaac tgtcttttaa ttccggcttc cacaaatcat cccaatgctt aatttgattt
    10981 gcatcaaaga atttatcgtt atatacgatc cctaaagttc cccaaaaata tggaatcgaa
    11041 tactgattat ctggatcaaa atccaagtct aaaaatcgtg gatcaatatt atttaatcct
    11101 ttgatcttcg aatgatctaa tggaagtact agtttttctt tgatcatttt ttggatcata
    11161 tattcacttg gtatagcgat gtcgtagttt gtcccacctt gctggatttt tgtgaacatc
    11221 gcttcattag aatcaaatgt ttcataattc actttgtatc catattcttt ttcaaatttc
    11281 gtgatcaaac taggatcgat ataatctccc cagttataga tggtaagtac ctttgctcca
    11341 ctcatgccac ttgttttttc aagttgacga acaccaagca gcagcaccgc aataatgatg
    11401 agaataccta aaaacagaga ttgtaatttt ttcatcgcag ccgtgccacc ccctcgcgta
    11461 ctcttctgtt tttcttcagc cgttttggac ggttttcttt actaataaag tagtatccaa
    11521 tgaccaatat catggagaac aagaaaacta acgcacttag cgcattgatt tctaaactga
    11581 ttccttgtct tgcacgagaa tagatctcta ctgagagcgt cgtaaagcca tttcctgtga
    11641 cgaaaaatgt aaccgcaaaa tcatcaagag aataggtaaa tgccataaaa tatccagcta
    11701 tgattcctgg agaaagataa ggcaaaatga tatttttgac tacttggaaa ttattagcac
    11761 caaggtctct agccgcatcg accattgaat cgttcatttc ctgcaatttt ggcaagacca
    11821 tcaagacgac gattggaatg ctgaaagcaa tatgggaaag caatacactc tgaaaaccta
    11881 atccgaatcc tagtagtgta aagaaaatca agaaactagc accgataata acatcaggag
    11941 agactaataa gatgttattc atgctcaaca acgctgtccg tgcacgtcgt tttttcgtgt
    12001 aatagattcc catagcccca aatgtcccga tgatcgtggc gatcaatgca gataagaaag
    12061 ccagcatgaa cgtattcaat acgataatga tcaaacgtgt atcttcaaaa acagcttcat
    12121 aattttccca agtaaatcct gtaaagctgt tcatcgttcc tccttcattg aaggaataaa
    12181 aaatcagata gaagatcggt gcatataaga ataaaaagac gatccctaaa taaagatacg
    12241 accatttaaa ttttttcatt tatgagcgac ctcctttttt cttctcgcct gtcaggagca
    12301 tcacgatgaa catagcaatg attaaaatca caccgattgt agaacccatc ccccagtttt
    12361 gtgtaaccaa gaaatgctgt tcgatagccg ttcctaaagt gatcacccga tttccgccaa
    12421 tcagacgagt cagcatgaat aaagaaagag aaggaataaa tactgcttgg acaccacttt
    12481 tcacaccatt taatgataac ggaaaaatca ctcgtcggaa ggtctcaaag ctattcgcgc
    12541 ctaaatctct actagcactg atcaaagaag gattcatctc ttccaatgca ttgaaaattg
    12601 gcataatcat aaatggaatc tcgatatacg ttgccacgaa cagaaaactg aagtctgtga
    12661 ataatatctg tttcggcccg atcccgataa aatcaaagaa atgattcacg ctgccgtgca
    12721 cactgaaaat cccaataaat gcgtaagctt taagcagcag attcacccat gtaggcaaaa
    12781 taatcagcat cagccataat tgcttgtgct tcagttttgt taaaaaataa gcagtcgggt
    12841 aactgatgat cagcgtgaaa aacgtgatca aaaatgcata ccatacagaa ttgatcgtca
    12901 tagaaagcga tgtccccgac gtaaaataag atagataatt attcagcgtg aattgcccat
    12961 tcatatcaaa aaaagattga taaatgatca ttaatacagg cgcgatcacg aataaaccga
    13021 gccacaaaat ataagggacg gaatagatcc tgcgcattgt tttcattctt gtccccccta
    13081 ttcttcgtag ctgtcaagac gtgcatcgaa ttcttcttca gactcgttga aacgcatgac
    13141 atggatatct tctggctcaa aggccaatcc tactttgctt ccttctttag cttttttcgt
    13201 tgaatggacc atccattcat tttgctgttc atcataacag atgatttcat aatggacacc
    13261 acgaaacagc tgagtatcga ccgttacgac tagttttcct ttatctggtg tcgtgatcgt
    13321 caaatcctct gggcgtaaaa caacttcaac tggttcattt agacgcatcc cgccatcgac
    13381 acattcaaat tgcttaccaa caaactcaac tagattatct tcgatcatta caccgtccac
    13441 gatattgctc tccccaacaa aatcagcaac gaaatgattg attggctcat cataaatatc
    13501 tacgggtgta ccactttgga cgattttccc tttattcatg acaaaaatct catcactcat
    13561 tgccaacgct tcttcttggt catgagtaac aaaaataaac gtgattccta atcgttgttg
    13621 cagatcccgc agttcatact gcatatctgt acgtagtttc aaatcaagcg ccgataacgg
    13681 ttcatctaat agcaatactt tcggttcatt tacaattgct cgtgcaatcg ctacacgctg
    13741 tctttgacca ccagacattt cactaatctc ccgtttttcg tatccaggaa gctggaccat
    13801 acgcaaagca tcttttacct tcttttcgat atctgatttg gacattttct tgatcttcaa
    13861 tccaaaagca acgttatcaa aaacattcat atgaggaaaa agtgcataat cttggaagac
    13921 tgtatttact tgtcgcttat tagctggcaa atcattcaac cgtttccctt caaaatagat
    13981 gtccccttct gtagcttcgg tgaatccagc tataatacgt aggatcgttg ttttaccaca
    14041 gccggaagga ccaagcagtg tataaaattt tccttgttcg atctcaaaac ttactttttt
    14101 taatactgtt tcatcatcat agcgtttcac tacttgatca aatgaaatga ttggtttccc
    14161 cacgttttta ccccctatat tacaaatatg attgcgttgc aacaatcaat acttttgttc
    14221 gttttttgag atgattcctt aattgatgtt gttttgtcgc ttgataatag atcgactgtc
    14281 ctgcttttgc cacatagacc gtctctccta agcttaactc taattcccct tctaatacaa
    14341 aaataaacgt ttcagataat gaaggttcaa acgtcttata ttctcctgca ccttcaaaag
    14401 tcagcaagac aggctccatt tctttttcat tcgaatcagc cacgagccat tccaattcat
    14461 acccgttttc ttcatcgaaa taaaccgtat gatcttcttt tgaataaacc acttgcagat
    14521 tcgctgcttc ttgatgaaaa aattcttctg gagtcacacc taagacttca agaatagaaa
    14581 agaatgtttc cattgatggt gaacttaaat ctcgttccaa ctgggaaata tacccttttg
    14641 acaaatctgt gcgttcgcct agttcttctt gtgtcaaatt cttttggatg cgcaaatttc
    14701 ttaacttctc accaatttcc ataatttcct ccgaatcgaa gttttctaat agtaaacttc
    14761 ctgtttacta ccactcgtat tagtatacga aatttattga aaaaggcaac cctttgtaaa
    14821 attttttttg ctgaatattt ctttatcttt tggaataaat tctagttgtt tgattcttta
    14881 tttttcacct aatttttgtc taaatttacg gaagaacaag aataaaagag taggctttat
    14941 ggtatgataa aagaaaattt taaaaggagt gtctcacgaa tgaacgcttt tcgatctttt
    15001 caggtaataa aggaacctgt tttccataca gcaatcagtg atctgccatt tactgagttg
    15061 gctgaaaatg aagtcttggt aaaaattgcc tattctgatg tcaattataa agatgcattg
    15121 gcaatgagcg agtctggtca ggtgatacga acatatccga tgactccagg gattgattta
    15181 tccggaactg tcgtccaaag taacaatcca cgtttttcaa aagaagatct tgtacttgct
    15241 actggttttg ggcttggcgt cacccatcca ggcggttata gccaatatca aaaagtacca
    15301 ggagactggt tagttcctct accaaaaaat atgacgctgc gtcaagcgat gatccttggt
    15361 acagcagggt ttactgctat gttgtgtgtg aacgctttaa tccgtcaagg tatgacctcc
    15421 gataaaaaag ttgtcgttac tggtgcttct ggcggtgtag gcagtacagc gatagccctg
    15481 ttacataaac taggattcca gtcaatcatt accttttcca gaaaagaaga gtctgtcaca
    15541 tggctaaaaa gtctaggtgc tttgcaagtc gtacgacctg atgaatttct gccagagaca
    15601 acaaaagctt taggaaaaca gcaaatcgac tatgtcatcg acacagtagg tggcgagcag
    15661 ctaagcagtc ttcttccatt gatctcttat aacggtgcag tagctttatg tggcaatgct
    15721 gggggaatca aactaaacgc gactgtcttg ccttttattc tgcgaaacat ccagttgatt
    15781 ggaattgatt ctgtcaatgt accaatagat caacgtcttt ctttatggca acagatggcg
    15841 gatttgcaga ttgcagatga actggtcgtc caagagatta ctctggatca attgcctgaa
    15901 acagcatcta aactacttgc tggaacacac caaggaagaa cactagtaaa tgtaggtgac
    15961 cataaatgaa gattctaatc acagctggag gaacgactga aaaaatcgat caagtacgtg
    16021 ctattacaaa tcattcgaca ggacgattag ggcaagcatt agctgattat ttagctgcca
    16081 atccggatac cacaatcgat tacgtgacta cgaaacaagc actgaaaccg aaaagacact
    16141 ccaatataac gatttatacg atcgaatctg cactggactt atttttacaa ttagaagaac
    16201 taacgaaaaa agaacactac gatgcgatta tccacagtat ggcagtcagt gattttactc
    16261 ctgcttttag tttttcagaa gaacagctgg caaaaaacct acccacttct tcaacgcaag
    16321 aagaactgga caattggttt gcggaaaatg aacagacaga taccactgtt tcaaagatct
    16381 cttctaacac cgagcatctt gtgctcgttt tgaagaaaac accaaaaatc atttcttatc
    16441 tacgtgaatg gcagccaaaa gcaaaaatca ttggtttcaa actattagta gatgtaccaa
    16501 aggaatctct tctggcagtc gcaaaaaata gtctgataaa taacaaaact gattttattt
    16561 tcgcaaatga tttgacggaa atccatggcg agacacacca cggctatctc ttatctaaag
    16621 atggaacagt ggaagaagct caaagtaaat ctgagattgc tgcattgatt actgagaaaa
    16681 ttagactgga ggaatcaaaa tgaaaaagat cttactaggc gttactggaa gtatttctgc
    16741 ttacaaaagc gctgatctta caagtcaatt cgtcaaatta ggtgcccagg tagatgtgat
    16801 tatgtctaaa agcagcacac attttatcac gccattaacc ttgcaatctc tgtcgaagcg
    16861 cgcagtccat acagacgtca tgcaagaatc aaatccgcag gtaatcaatc atatcgaact
    16921 agcaaaacaa gctgacttgt ttctcatcgc gcctgctaca gcaaacatta tcggcaagct
    16981 agcaaacggc attgcagacg atcttctttc aacagtcgca atggcgcttc cagtgggaac
    17041 accaaaattg attgcccctg ccatgaacac gaatatgtat caaaacccga tcactcagcg
    17101 taatctggaa acattgagat ctgtcggcta tcaggaaatt gaaccaagag aatctctttt
    17161 agcttgtgga gattttggaa aaggtgcctt agctgatatt cagacgatca tcgaacaggt
    17221 catgctgact cttaatgaaa gaaatgctta gaaaaaagga gtacttatga aaaatacaaa
    17281 aaactttaca ttaactgcga tgtttttagc tattttgatt ttattagctg ttacgccttt
    17341 aggttttatc cctatcggtc caatcaatgc aacaaccatg catattcctg taatcattgc
    17401 ttcgatcgtt ttaggaccaa gactaggtgc ttttcttggc ggtacatttg gactgatcag
    17461 catgatccgt agcacagtta tccaaacacc tttgtctttt gtcttttctc cgtttatccc
    17521 agtcatcgga acagaacatg gaagctggaa agccttgctg atcgcgttta ttcctcgcat
    17581 cttgattggg gtggttcctt atttcgttta taaaggtctg cgcaaactaa tgaaacaaaa
    17641 agcagattcc gtttctttat tcttagctgg actttccggt tcattgacca atacgatttt
    17701 agtgatgaat atgatctact ttttattcca gcaagattat gccaaagtca tcggaacaaa
    17761 tttaaatgct gtttattcag ctattctagc ggttattttt acaagtggta tacctgaagg
    17821 aatcgtcgca ggtcttgcga ctgcagcagt atgcaatgtc ttgttaagat acttcaagca
    17881 cccttctctt actaactgat agtcatcgta gccgatctct gatttttttc tcttttggtg
    17941 ttactattga atggaaagat ttgacgtcga aaggatgatc caaatgaaaa tttttgttgt
    18001 aggtgcaaac ggacaaattg gccgacacct gataaaagat ttagctccaa gttcccatga
    18061 aatttttgca ggggtgagag atgttgcttc ccaaacatta gtaaaaaaag agaatgtttc
    18121 gtatgtctct tttgatttaa cttggtccgt ggaaaaaatg accgaagcat tcaaaggcat
    18181 cgatgtattg atttttgctg ccggctctca aggaaagaat cttctgcaag tagacttgga
    18241 tggcgcaatc aaaacagtga tcgcagctga aaatgcgcat gtctctcgct atctgatggt
    18301 cagtgctgtt tatgcagatg aaccagcaaa atggcctgag tccatgacag attattatat
    18361 tactaaacat tatgcagatg aatggctgaa acgaactaac ttagattttg tcattcttca
    18421 accagtcaca ttgactaacg atgaagaagt tacttccatt caattaacta agcctaatga
    18481 gaaggcatca aaaacgatca ctcgctctac agttgctgct gtacttgccg ctctagtgga
    18541 agaaacggat atttctcgta cgactctagt actttctgaa ggtagcaaag agttgaatac
    18601 agcattccaa gaatgggcaa aggaggaata gatccatgcg tgccatttca atcaagcatc
    18661 ctggttctgt agatgtatta gaacaaattg aagtgcctgt tcctaaacca aaacaaggcg
    18721 aattactgat caaagtccat acagcagctg tcaatcgaac agatattatg aaaagagaat
    18781 cagtctcttt gaaacctccc taccctattt tgggagtcga agtagctgga gaggtagttg
    18841 agaataaaag tgatatctcc acttttactc ctgggacaaa agtatacgga ttagtcaatc
    18901 taggtggtta tgccgaatat gctgtgatgc ctgccgatcg tgcaatcctt ctccctgata
    18961 cgctagacta tgtttctgct gctggaatct cagaagtttt tttgactgct tatcaaacac
    19021 tttactggct tggaaaatta caagaaggcg aaactgtctt gatccatgca ggagcaagtg
    19081 gtgtgggaac agcagcgatc caattagcta gacagctttc tcacgcgaaa atcatcgtta
    19141 ctgcaggatc agaagaaaaa ctcgcctttt gtaaagaact tggtgcagat gaattgatca
    19201 actataaaaa acaagacttt caagaagagg tacgaaaaat cactgaaggt cggggagtcg
    19261 atgttatttt ggatttcatc ggcgcttctt actgggaaaa gaatttagct tctatagccg
    19321 ttgatggacg ttgggtcttg attggtatgc taggtgggac catcgtgcct gagatagacc
    19381 ttggtgtatt gattgggaaa cgtgtccaac tgcttggtac attattgaca cctagaagtg
    19441 atcagtataa agcagagctc gcacaaactt ttatgcaaaa agctgggcct tttctagaaa
    19501 atggcaagat tcggccaatc attgaccgta cattccaatt cagcgcagtc aaacaagcgc
    19561 atgaatatat ggaagcaaac aaaaacatcg gaaaaatcat tttgaagata actgaataaa
    19621 atcatgctga tcaaaaaaga ggctgggaca gaagcgttta actccaagaa ataagaagaa
    19681 attcacgaaa attgcttttc aaatttttgt gaatttcagc ttatttccga aggaatttct
    19741 tctgcttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt tacttttgtc
    19801 ccacgctctt tttccgaata aacggtgttc aaaagcttgc atctgcggta ataagtccaa
    19861 ccaagttaaa aacataagaa gctgttttta cttggctgtt cttattactt gatgcagggc
    19921 agctttttca caacctcttc actaaacagt gttcaatcag ctgaccctcg gtattagctc
    19981 aaaacgctga tttcacaacc tctttttatt tacgttctat ttcattcatt ttccgaagca
    20041 aatcgagctg cttgtatgcc cgcttgtcgc ccaaaaataa tgatctccgc aactgaattt
    20101 ccaccgatcc ggttgtttcc atgtaagcca cctgtgactt caccagcagc atataacccc
    20161 ctaattggct ggttttcttt atctaatact tcagtgttcg tattgatttt cacaccgccc
    20221 atcgtgtaat ggattcctgg tgcgattttg atcgcataat aattcggaga cgataagtca
    20281 tgttccatgg ctgtggcacg gttgaattga gcatcttttt tctccgcaac atccgtattc
    20341 catccatcaa ctgtttttgc tagtgtttct tttggtaaat cgattttttc tgctaattct
    20401 tccattgtct ttccagttgt gacatatcct ttttggtcat aaaattcaat agctgtggca
    20461 cgatcacgca cgccttggtc aaaaatcaaa taagctgatt tttcaggcaa tgctgtaatt
    20521 gctgcagata ctttatccct tgtatccatt tcattgacaa atcttttccc ctcttgatcg
    20581 accaagattg ctccttctcc gcgaacaacc tcaccaatca agaccccctc atcctgctgc
    20641 acagttggat gaatttgtat tttatccata tcaacggttt gtccacctaa tttttcaatc
    20701 atggtgatac catctccagt tgatccttct tgatttgtcg taatataatc agctaaatcg
    20761 ggacgatatt tttcaataaa ttctttgctg gcaccaaaac cgcctgtggt aactacgatt
    20821 gcttttccgg tgacctcttt tggttcttct ccttgaacat gtacagtcac ttcattgacc
    20881 gtttccccat ttttcttaat gtctgtcaca tctgcgttga caaaaatagg gatttcccgc
    20941 tcatggacgt tattcaataa cccctccact aagtatccac caatagccga gccatcagac
    21001 ggacgatgtg tcctcttttc actcatccct ccagtgatcg ttaagttgtc cagtttgata
    21061 ccgttttgat ctagccaatc gaccgcatca gcagagtgat cgacaaagta tcgaagcatt
    21121 tctttatcat tcgttcctcc accacctttt agcgtttctt catagaaggc atcattcgag
    21181 tctttgattc cttcttcttt ttgaaatttt gtttctgaag cattcatacc gcttgatgat
    21241 ttagatgtgt ttcctccggc aacaggcatc ttttccaaaa ttactggatt caacccagca
    21301 tcttttgctt cgatagctgc tgccatccct gcaccaccag ctccaataat cacgacatca
    21361 taagattctt tcatttcttt tggatctgta tatacttgtt cagaagcgcc tgatgatact
    21421 tcggcagatt ccttcgtact agaacttgtc tgcgctgtat ttttctgatc tgcgccgcat
    21481 gcgctcatag caagaagtgc aacggctgtt aataaaccag caactaattt tttcatttgt
    21541 atcccccttt tctccttata ctatattaag tgattttttt cacaatatca actctgtttt
    21601 tctttaagtc atatagcttt ggtaaacaca cgcttcttag tataattaag gatatacatg
    21661 aaggagttat tttatgaatc atttgaaaac cttttttacg aacagtacaa aaactgcagg
    21721 tagaatcaca aacggaatct atattttatt acgtatcatc gtcgttatta tgctagtgga
    21781 tcaggtctgg aaccaacact ggggaaatac aatgctttta ttactgacat taattttatt
    21841 tgaaatccct tattttctgg aaaagatatt aaagatacag attccaaata tgctagaaat
    21901 cattttgtta tcttttattt ttagttcaac agttttagga gagttaagtg atttttatgg
    21961 ctatttttca atgtgggata cttttctaca tgcattcagc ggatttttag ctgggggtgt
    22021 cggcttttcc cttgtttatt tgctcaataa aaatacaaaa accatgaact tgactccttt
    22081 attactagca gttgtttcct tctgtttttc gatgacagtt ggtgttatgt gggagtttgt
    22141 ggaattttca gcagataaat tactgcaatt cgatatgcaa aaagatacga tcatccagca
    22201 aatcaatagt gtggagttaa gtaaagacgg aaacactgtc tatcatgttc cagatatcac
    22261 tcggacaatc attgaaagtc aaaatgcaga tggtaaaaca gataaaacaa tcatccaaaa
    22321 tggttactta gacgtgggaa tcatggatac tatgaaggat ttatttgtta attttattgg
    22381 agcagttgtt ttcagtgtat ttggttacct ctatagtcga aacgattacc aaaaatttac
    22441 gttcatcaag aattttattc ctaaaaaaat caaacgataa aacatgctaa aaaacaagag
    22501 aaggggcagt gacaatactt ttgtcactgc cccttctttt ttgaatgtat ggcatgccat
    22561 tagttatccg atagtattaa ctcaaaaaag taagatatga ggaatgacta tttcgttttt
    22621 tatcccaata ctgaggactt atttcaccat ttcgttattt atctaattta cttaaaacct
    22681 gcataacttt ttcataatct ggattaggtg tgccgtctag attttcagca ataaaagtaa
    22741 aacccatttt tttataaaga gcaatcgcct gatggctcca tgtctgtgtg tgaagataga
    22801 ttggtccgtc cgtatggagg gtaggaaatt cttgcaagac tctggcagtc aatgcttttg
    22861 cgagcccttt tccttggtaa tctggtttca ctgctaacca atgaaaaacc ggatacactt
    22921 gctctcttga tttttttcgc caagcggtac aggttgctac tttttctcct ttttttgtct
    22981 ctacaaaaaa catccttctt tcaagttctt caggataagg agcaaagttt tgttgaaaat
    23041 actcaagtgc ttcatctgtc gtttgaaatt ccccaacgga agtctcaatc tccgcccaag
    23101 cagattcatc ttctttttga taaaatcgaa attcaaaacc ttccgctaaa ggcgtgattg
    23161 gaacattttg ttgaagtggt cttgtcatcc agatttctgc gtaaggtaac gttttatcta
    23221 acatttttct atccctcctc gtctccatta tgccaaataa catatgactc agctacttat
    23281 ttgactataa cagatagaga tataaatcag aaccagtcat ctgcgtcaaa ttcatttcga
    23341 ttagcgtata cccatgttcc tgccatacct cgcatgaaat ctcctaaagc agaagaagga
    23401 atacctccac caggtgcggg tgttcccgga ctaggaagtg gtacttgcgg ctgcagatgc
    23461 tcatcggtgg aatcaatagt tgacaagcgt tttttcttcg tttccttctg gtcatctgat
    23521 tccttctgat cttccgactg tttagcaaca aattcttttt ctgattgttg ttttatttta
    23581 ggctctaaaa tttcattttc caatccagaa acttgtgggg ccaaatgagc ttcttgcctt
    23641 atcgttttat tagattctga gatttttcta gatattcttg ctaactctag gcttcttgtt
    23701 tgttttactt tccctgttgg agaaactgca tgagccattt gttgtttttt tctgacaagc
    23761 tttcttcttg tcttttttgt tctttttgat ctatctgttt gtttaatggt aaaacatgta
    23821 atagtgactg agcatggagt tcttggagtg aatacgggtc atgataacta tacataatcg
    23881 taaaatcacc tgtttgtttg gtcaaataac catcctccaa tggtacttca ttgattgtaa
    23941 ccgatacatc ctttaacggt acgggtttac ctaaggaatt tgttgctgat tggatcaact
    24001 cttctttgtt tattttttcg tttggatgaa caataagatc atgaccttct aatgaacgat
    24061 tgtctggtat ataaacataa gtaaaggtaa tttcccctcc tgattgcttc ctattattca
    24121 tcagttctga gtgaagtact tcaaggatct ccgaccagtg actttttctg tctaatccgt
    24181 ttgcttgcat ccaatcagaa atagaagtga ctttcccatt ttgttcataa gtcgataaat
    24241 ctatatttgg cactcgatca gaaaattcag caggtaggcg caaatcagga atacgattta
    24301 cactactcat atgtgtttct gcttgaagtt ctttatttgt ttgactatct ataaaatgag
    24361 tgatgacagt cgatgaagca gcagatgctt ctacataaac tacagctgtt gtttctgcca
    24421 catttgatgt tttcgagtct atataagtta atacaacggg atattctccc ggagttgtag
    24481 tatctactgt cccgttcatt ttgatttctt ctttatttcc tgaagttcca tcgctttttg
    24541 ttacatattc aacaagatct ttaggggcgt aactctctcc tgttcttaag tgaagatctt
    24601 ttcctttgat agctgtttga tcagcacgaa catggatcac agcagatgca gtcattgttt
    24661 ttaaagcaat tggatcaaaa taggacaagg aaatagtaga tgttcctgta ttagtcgtat
    24721 ccacgatatt cccattgttt gaaattactt tggacatatc tccttgtgtt ccatctgcgt
    24781 tcttcagcct ttctaccaga tcagatatcg aaacttttgt ccctgccaga acatcgattt
    24841 tctttctttc aaactgtgaa taatcttttt catatacata agaaacttgt gcgcttccac
    24901 cgtcctttat atctgtcaaa gcatgattca aaatatcagt gatctgtttt aactgatcac
    24961 cttttgtagg atcagtcata tcggaaaaga tagaaagcgg cacactgctt ccggtttccg
    25021 atgtccataa tgttttgtta ggaattaacc agtagtggtt tatcgttgga ggataactga
    25081 ctttattata ggaagtcaat gcaggaagta gctgttttcc atttgcatca gtgaaagtat
    25141 gagtaatagt gataggggaa tcagtggtct cagaaacaga ggtacgtacg accttgatgg
    25201 tttgtgtcgt tgcctgaacg ggtacatgca tatagacacc tacagataaa ctgatcagca
    25261 tcgtgcgcat cataaactta acgtagtgtt tttcagaaca cgaccgttcc ttatctaaac
    25321 gacaagcgtg tttatttagt tgttttgaac gtctttttga acacatttat tcttagtcct
    25381 ccctactctt gtctaaacat gaagtagtaa ttttcataag atgatttcat tatataaata
    25441 aaatcaaaag aagatggact attaggaga
//
LOCUS       DAYYGO010000029        24587 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000029 DAYYGO010000000
VERSION     DAYYGO010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.029"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            111..1253
                     /locus_tag="ACHA5T_001000"
     CDS             111..1253
                     /locus_tag="ACHA5T_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM8886592.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            1246..1452
                     /locus_tag="ACHA5T_001001"
     CDS             1246..1452
                     /locus_tag="ACHA5T_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFM8886593.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            1531..2724
                     /locus_tag="ACHA5T_001002"
     CDS             1531..2724
                     /locus_tag="ACHA5T_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8886594.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            2929..4068
                     /locus_tag="ACHA5T_001003"
     CDS             2929..4068
                     /locus_tag="ACHA5T_001003"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFM8886595.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            4061..4861
                     /locus_tag="ACHA5T_001004"
     CDS             4061..4861
                     /locus_tag="ACHA5T_001004"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8886596.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            4861..5688
                     /locus_tag="ACHA5T_001005"
     CDS             4861..5688
                     /locus_tag="ACHA5T_001005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8886597.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            5693..6400
                     /locus_tag="ACHA5T_001006"
     CDS             5693..6400
                     /locus_tag="ACHA5T_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8886598.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            6500..7366
                     /gene="rfbA"
                     /locus_tag="ACHA5T_001007"
     CDS             6500..7366
                     /gene="rfbA"
                     /locus_tag="ACHA5T_001007"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFM8886599.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            7380..7952
                     /gene="rfbC"
                     /locus_tag="ACHA5T_001008"
     CDS             7380..7952
                     /gene="rfbC"
                     /locus_tag="ACHA5T_001008"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFM8886600.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            7974..9002
                     /gene="rfbB"
                     /locus_tag="ACHA5T_001009"
     CDS             7974..9002
                     /gene="rfbB"
                     /locus_tag="ACHA5T_001009"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFM8886601.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            9100..9951
                     /gene="rfbD"
                     /locus_tag="ACHA5T_001010"
     CDS             9100..9951
                     /gene="rfbD"
                     /locus_tag="ACHA5T_001010"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFM8886602.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            9985..12018
                     /locus_tag="ACHA5T_001011"
     CDS             9985..12018
                     /locus_tag="ACHA5T_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886603.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            12062..13342
                     /locus_tag="ACHA5T_001012"
     CDS             12062..13342
                     /locus_tag="ACHA5T_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFM8886604.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            13552..14358
                     /locus_tag="ACHA5T_001013"
     CDS             13552..14358
                     /locus_tag="ACHA5T_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8886605.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            14370..15590
                     /locus_tag="ACHA5T_001014"
     CDS             14370..15590
                     /locus_tag="ACHA5T_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8886606.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            15580..17166
                     /locus_tag="ACHA5T_001015"
     CDS             15580..17166
                     /locus_tag="ACHA5T_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8886607.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            17205..19343
                     /locus_tag="ACHA5T_001016"
     CDS             17205..19343
                     /locus_tag="ACHA5T_001016"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8886608.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(19711..20694)
                     /locus_tag="ACHA5T_001017"
                     /pseudo
     CDS             complement(19711..20694)
                     /locus_tag="ACHA5T_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
     gene            21072..22463
                     /locus_tag="ACHA5T_001018"
     CDS             21072..22463
                     /locus_tag="ACHA5T_001018"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFM8886609.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            22479..23519
                     /locus_tag="ACHA5T_001019"
     CDS             22479..23519
                     /locus_tag="ACHA5T_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8886610.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            23538..>24587
                     /locus_tag="ACHA5T_001020"
     CDS             23538..>24587
                     /locus_tag="ACHA5T_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFM8886611.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTT"
BASE COUNT         8024 a         4090 c         5088 g         7385 t
ORIGIN      
        1 ccccaaacct gtgagtagta atgagaggtt ttaagaagat agtacatatc acactttttg
       61 aaaggtggtt tacaggtatt tcaaggctat tgtgggattg gaggaataca atgaacaatt
      121 tagaatttgc acttgaaatg aagaacaaac gtcttgccag cggtctttca caaggagaac
      181 ttgcaagtct tacccatgta tcaagatata gtatcaaccg ctttgagaac ggaaaagcta
      241 acgccagcaa ggaaacacag aacattatcc tgcgttgtct gaattactat atctgcgata
      301 agccttttta tctgctcgtt gattatctgt ctgtccgttt tccaacgact gacgcattgg
      361 aagttatccg aaaggtgctt ggcatgaaag cagattattt tatccattat gactatgggt
      421 attatggcta taaggagcat tatgcttacg gggaaatcaa ggtcatggct tctgatgatg
      481 aacacatggg cgtattttta gaattaaaag gggcaggctc acgcaacatg gaatatgtct
      541 tacaggcaca gaacagggac tggtattcat tcttaaaccg ctgtcttgac tgtggcggtg
      601 ttatccgacg ttttgacttg gcaatcaatg atatgtgcgg tctgctggat attcctgtgc
      661 tatctgaaaa atacagaaat ggtggtgcgg aatgtcgctg taaaaactat gaaaatgtac
      721 aaggtggaaa attaagcgga aaaaaccgta atttagcaag taccctttat atcggctcaa
      781 aggcaagcac aaaatatttc tgcctgtatg aaaaacaaaa ggaacaggca acgaaaaaga
      841 aacataccga tattatcaac cgttttgaaa ttcgtctgcg tgacaaaaag gcggtacagg
      901 cagttgaaga actgttattg acgtataacc ctcatgggct ggtgttttat ctcattaccg
      961 attttgtgga atttcctgac tatccattgt gggagatatt catttcccat gacagtttac
     1021 cttttgaaat gaaccctgta cctgtcaata tggaacgcac tctgcaatgg ctggaaagac
     1081 aggtcatgcc gtcaatcgtg atgatagagg aaatcgacag gctgacaggt tcaaactaca
     1141 tgaaaatgat tgatgaatgt acccaccttt ctgaaaaaca ggaaatgctt gtggaacaga
     1201 tgtgtacgga catatcagac gtaatcgaaa gtgagggggt gttttatgag taatacacag
     1261 gatattcccg tatgggaaaa atacaccctt accattgaag aagcgtcaaa gtatttccgc
     1321 attggagaaa acaagttaag acgattggca gaggaaaaca aggacgctgg ctggctcatt
     1381 atgaatggca accgcataca gattaaacgc cgacagtttg aaaaggttat tgataaattg
     1441 gacgcaatct aatgcaaatg agccttgtat gtgttatgat gaacacaagt catatcaagg
     1501 ctctttccaa caaggaaagg agcagacacc atgaaagaaa aaagacggga taacaaaggt
     1561 cgtatcctgc atactggaga gagccaacga acagacggaa aatacttata taaatatgta
     1621 gacgcattgg gaaacacaaa atatgtgtat gcttggagat tgacacccac agacccgaca
     1681 ccaaagggaa aacgggaaaa accctcactt cgagaactgg aacagcagat aagacgggat
     1741 attgaggacg gtatcgacag cacaggcaag aaaatgacgc tttgccaact ctatgccaaa
     1801 cagaacgcac agaggacaaa tgtgaagaaa agcacacaga aacaacggga acaactcatg
     1861 cggttattaa aagaggacaa gttaggtgct aggagcattg atacgataaa accctctgac
     1921 gctaaagaat gggcgttacg catgaaagac aaaggctttt cctacaatac cattaacaac
     1981 cataaacgct cgttaaaagc gtcattctac atagccatac aagatgatta tgtgaggaaa
     2041 aacccttttg atttcaagct aagtgaagtc atagaaaatg ataccaaaga gaaagtcgca
     2101 ttgacagagg aacaggagca agccttattg tcatttatta agacggacaa cgtgtatcac
     2161 aagcattatg atgatgtgct gatactgtta aagacaggac ttcgtatctc ggaactgtgc
     2221 ggactgacag tagccgacat tgatttcaag aatgaagttg tgattatcga ccaccagtta
     2281 ctaaagagta aggaacaggg ctattatatt gaaacgccta agacgaaaag cgggataagg
     2341 caagtgccat taagcaaaga aacgatacag gcatttcaac gggttatcaa cagccgacca
     2401 aagataaagt cgatagaggt agacggacac agcggttttg tgtttatcaa tccgaaaggt
     2461 aatccaaaag ttgccattga ttataacgcc ttatttatcc gtatggtaaa gaaatacaac
     2521 aagcaccaca aggacaatcc cttgccacat attacaccgc atacgctacg ccatacattc
     2581 tgcacaaggc tggcaagcaa gaacatgaac ccgaaagatt tacagtatat catggggcat
     2641 tcaaacatca gtatcacaat gaactggtac gctcatgcgt ccatagatac cgcaaaatca
     2701 gaggttcagc gtctaatcgc atagaagtat ttaccacgat tttaaccacg attgacaggg
     2761 aaaatataag aagatagacc tagatatgcg aggtttacca caaaagcaaa atgcccgtag
     2821 agccgataaa ataaggcttt gcggacattt gagaagatat aaaaagatag tcaaaaagac
     2881 atatataatt tataataatt atgactctta attaaagtag gtgcccatat gcttgtttct
     2941 tttagtatgg ttataagatt ttttctgaca attttattat cactgatatt aacacctgta
     3001 ttcaaaatca tttcagtaca aacaggtatg gtcgacaaac caaatgaaag aagaataaat
     3061 aaagttccaa tgccttcagc gggcggactg cctatcttta tttcatttgt tatatcgact
     3121 ttattcttat tccgtaaaat tatccctcaa tcgtatattg taccgatctt gattgcttct
     3181 ttagtaatca tagtcacagg agtgctcgat gataaatatg agctgacacc taaacaaaaa
     3241 agtataggga tcttgattgc ttctcttatt atttattttg tagcagatat acgtattgat
     3301 tccttcacgc ttccttttat tggatatatc caactaggct ggcttagttt tcccgtaacg
     3361 attttttgga tcttcggtat cacgaatgcg gtcaatctga ttgacggttt ggatgggctg
     3421 gcggcaggga tttcattgat cggtttaatt acgataggaa tcattggata ttttttcctc
     3481 catgcttcta cagtgtatat tccagttgtt attttctgtc ttgtagcaag tattatcggt
     3541 ttttttcctt acaattttta tccggcaaag atttatttag gcgatacagg agctctattc
     3601 ctagggttta tgatggccgt cctttcacta caagggctga agaacgtgac ttttgtttca
     3661 tcgatttctt tgctgatcgt catgggagtg ccggtaacag acacgttttt tgctatcatt
     3721 aggagaaaag caaaccgtgt ttctttttct acagcagata aaaaacatct tcatcaccga
     3781 ctaatcagtt taggctttac tcataaaggt gctgtcctga cgatttatgc gatggctttg
     3841 atgttttctt tcacagctat ggtcatgaat tatactggaa gaatcggaac gatcatattg
     3901 attattgcca tgttgtttgc tgcaatttta ttatttgaat tgattggtct gatcaatgag
     3961 aaacatcagt ttattttaaa aacattgcga tttttaggga ataaagagta ccgtacgcaa
     4021 gtaaaacaga aatatttgaa gaaatggaac cgtcatatag atgaataaca aattaaacca
     4081 gccgatcctt attagtattg tgacggcaaa cagtgataaa atttttcgga cactagataa
     4141 ttttatttca actgtaaaag atgaaacagc agtaaaaatc cgaatttttg acaacaattc
     4201 acttcccgag tacaaaaaaa gactaacaga gtataggaaa tatccctaca tcgatatcta
     4261 ttttcatgat gaaaattccg gatttggtta tggacataat cacaatctga tagctagcga
     4321 ttctcgctat ggcattattt gtaacccaga tattttagtg gatgaagaga cagttacagc
     4381 attggtcttg ttattgaaag aacatccgga atgtgcgatg ttagcaccta agattcttaa
     4441 tgaagatgga acaactcagc acctcatccg tgagcagctg gctgtttttg attacgtttt
     4501 gcgctttgtg cctttcaaat gggtcaaaaa aaagtttgac aaacgactgg ctgtgtttga
     4561 atgccgagga ttgcctactg atgctaatag ttatgtacgg atgatctcag gtagttttat
     4621 ggtagctgat attgataaat ttaaagagat aaaggggttt gatgagcgtt tcttcatgta
     4681 ctttgaagac aacgacttgt gtttgagttt tgaaaaagca ggttacaagt tattgtacac
     4741 acccttttat gcagttatcc atttttacgg gaagggtgcg catcgaaatg caaaactgtt
     4801 tcgcgtgttt ttgacttcta tgatgaaatt tttcaataag tgggggtggc gttttttctg
     4861 atgaaaccag aaatattagt agtcgttgtg ctttatcagc aactattcag tcaatctcca
     4921 gcttatggac cattaagcaa agcacttagc gaaaagcaaa ttcagcttat tatttacgat
     4981 aatagtcctc aaaggcagca aaatgagctg tttgaaaaag aaaatatcgt ttatcatcat
     5041 gatccgcaaa atcctggttt agcggtggct tataattttg cgttgtctca agcagatgaa
     5101 ggaacacgtt gtctcgtcac tctggatcag gatacgaggc tagtcgacga ttattttgaa
     5161 ctattgcgta aagtaacctt tacagatgaa tgtgttgctg cagttccgat gattttttct
     5221 gggagccgac aagtttctcc cctatattca gatcgttata tcaatcgtac agctcaagca
     5281 gtagaagttg aaggcccaac agctgaacga atcatggcaa tcaattcagg tgttgcctgg
     5341 tctgtcaaat ttctaaaaaa gattggcggg tttaatcctg ctttttcttt agattttttg
     5401 gatcattggc tgttttggaa agtggatcag ttggaaaaaa ctgtggagat ccttcctgta
     5461 cgaatggaac atgatctttc ggttttagac tatgaaaaag tttcaagtaa tcgataccgt
     5521 tctattttac aagcagaaaa tcgattttat catgaatatg atcaaaggca tttatccagt
     5581 caccgacgac aattattgtt aagaacagct aaacagttct tgaccgtcaa aaaccggcat
     5641 atctggcgga tgactttgcg ctcatatgtt aagaattgga aggtgtgaat tcgtgcgttc
     5701 ggtttgtata gctaccttta atggaggcag atatttaaga gagcagttag atagtatttt
     5761 atctcagctt tcagcagaag atgaagtagt catttctgat gatgggtcag tagatgatac
     5821 atgggatata ttaactgaat acgcagaaaa agataagcga atccagcttt tttcggggcc
     5881 tcgtcaagga ttgatcgcta attttggtta tgcgatccag cagacgaaag gagatctaat
     5941 ctttttggca gaccaagatg atgtttggtt ggagacaaaa gtgaaaaaga tcactgacta
     6001 ttttgagcag aatccggatc agctagtagt agtttctgat ttaatcattg tggacagcga
     6061 gctgcaagag atccatgatt cgtatttttc gtttcgaaat tccaaagaag gatggcttaa
     6121 taatgtgata agaagtcact atattggtgc tggtatggct ttccggtctt ctttaaaacc
     6181 cgatattttg ccgatcccgt cagaagtgcc gatgcatgat atgtggattg gtttgctggg
     6241 aggaaagaag actgggttcc taagagaacc gttaactctg taccgaagac atgatttaaa
     6301 cgcgagtgag attgaaacac attctaaatt acggcaaaaa atttcttggc gtgttcatct
     6361 gattacggcg ttgatcaaac gaaaatggtt taaacgttga taaaagatgt ttaaatcctt
     6421 aatatttcca tgacaaatat aacaattgat gagataataa caaagtgaaa tttgaaatca
     6481 aaaaggagag cagatatcca tgaagggaat cattcttgct gggggcagcg gaacacgtct
     6541 gtacccttta acaaaggcaa catcgaaaca attgatgccg atttatgaca aaccaatgat
     6601 ttattatcca atgtctactc tgatgttggc gggaatcaaa gaaattctga ttatttcaac
     6661 gccacaagat acaccacgtt tcaaggaatt atttggaaac ggaaatgatt taggactgca
     6721 gatcgaatat gcagtacaag aaagtccaga tggattggca caagcgttta tcattggaga
     6781 agaatttatt ggagatgata gtgtctgctt ggtattaggg gacaatattt actatggtgg
     6841 cggtctgtct aaaatgttgc aacgagcagc ttcaaaagat acaggcgcga cagtatttgg
     6901 ctatcatgtc aatgatccag aacgctttgg tgtagtagaa tttgatgaga atatgcaagc
     6961 tttgtcgatt gaagaaaagc ctgctcagcc aaaatcaaat tacgctgtaa ctggccttta
     7021 tttttatgac aatgaggtaa tttctatcgc taaaggtatc aaaccttcag aacgtggaga
     7081 actagagatt acggatgtca ataaagcata tttggataaa ggaaaactat ccgtagaact
     7141 tatgggacgc ggatttgctt ggcttgatac aggaacacat gaatcattgc ttgaagcatc
     7201 tacctttatc gaaacaattg aaaaacgtca gaacttaaaa gtggcttgtt tggaagaaat
     7261 tgcttaccgt atgggctaca tcgataagga acaattgctg gcccttgcac agccattgaa
     7321 aaaaaatcaa tacggtcaat atttattgaa tttggccgct gaataaggag tccttacaga
     7381 tgaaagtaac agatacaaaa ttaacagatg tcaaaatcgt agaaatggac gtctttggcg
     7441 atcatcgcgg tttcttcaca gaaagttatt caaaagcaaa atttgcagaa catggattga
     7501 aatatgactt tgttcaagac aaccattcac tatcagctga agctggtgtc cttcgggggc
     7561 ttcatttcca aaaaggagaa gctgcacaga caaaattgat tcgggtagtc actggagcag
     7621 ttttagatgt agtagtggat atccgcaaag gtagcccgac ttatggtcaa tgggacggat
     7681 ttatcttatc tgaacataat catcgacagt tgcttgtacc taaaggatat gcccatggat
     7741 tcgttacttt gacaccaaat gtgaacttcc tttataagtg tgataattac tataatgcag
     7801 aagcagatgg gggcatcgca tttgatgatc cagatttagc gattgattgg ccaattgatc
     7861 catcaagagc gattatgtcg gaaaaagatc accgtcatcc gacattaaaa gaatttgaag
     7921 cagaaaatcc atttgtttac ggagaaatct aagaattaag gagaaatttt cttatgaaaa
     7981 acatcattgt taccggaggt gccggattca tcggctctaa tttcgttcat tacgtggtaa
     8041 acaatcatcc agaagtgcat gtcactgttt tggataaact gacttatgca ggaaacaaag
     8101 agaacttggc tggacttcct agcgatcgcg tagaattggt cgtaggagat gttgcagatg
     8161 cagaattagt ggaccgtcta gtaaaagaag cggacgcagt tgttcattac gcggcagaat
     8221 cccataacga taattcatta aaagatcctt tcccatttgt acaaacgaat atcattggta
     8281 cgtatacact tttagaagct tgccgcaagt atgatgtacg ctatcatcac gtatctactg
     8341 acgaggttta cggcgatctt ccattacgtg aagacctgcc aggacatggg gaaggcgaag
     8401 gagaaaaatt cactgcggaa acaccatata atccttcaag tccatattct tctacaaaag
     8461 ctggttcaga tctgttagta aaagcatggg tgcgttcttt tggcttacgt gcaaccattt
     8521 caaactgttc aaacaactat ggtccttatc agcatattga aaaatttatt cctcgccaag
     8581 taacgaatat cttaagcggt attcgtccaa aattatatgg cgaaggaaag aatgttcgtg
     8641 actggatcca tacgaatgat cattcttcag cagtgtggct gattttgacg aaaggcagaa
     8701 ttggcgaaac ctatctgatc ggtgcagacg gtgaagaaga taataagacc gtgatggaat
     8761 tgatcttaga aatgatgggt caaccaaaag atgcgtacga ccatgtaaac gaccgtgcag
     8821 gacacgactt gcgttatgca atcgattcga caaaattacg ggaagagtta ggatggaagc
     8881 ctgagtttac gaacttccgt gatgggttgg ctgacacgat caaatggtac gaagaacatg
     8941 aagactggtg gaaaaaagaa aaagaggccg ttgaagcagc atatgccaaa aatggacaat
     9001 aatacagaga aaatgtcact gtattaatgg aaaaacacat gtgaggcagg cgctcgcctg
     9061 tctctttttt aaaataaaaa tcaaggagcg gataatcata tgtttttatt aactggagga
     9121 aatgggcaac taggtacaga attgcgccat ttattagatg aaaaaaatct gcaatatgtt
     9181 tcaacagatg caaaagactt ggatatcaca gatgccgaga aaacaatggc ttacatcaca
     9241 gaattaaaac ctgatgttat tttccattgt gcagcttaca cagcagtaga taaagcagaa
     9301 gaagaagcaa aagaattaga tgagaagatc aatgttgatg gtacaagaaa tgtagcactt
     9361 gcagcaaaag ctgtgaatgc tcaattcgtc tatatcagta cagactatgt tttcgatgga
     9421 aaaaacaaaa atgtagaata cgaagtagac gaccaaacga atccactaaa tgaatatggt
     9481 cgtacgaaat tattaggcga aaaagcagta gaagagattt tagaagacta ttatatcata
     9541 cgaacttctt gggttttcgg gatctatgga cacaatttcg tcttcacgat gcaacgccta
     9601 gcggaaaccc gcgacaaact gactgtagta aatgaccagt ttgggcgacc aacttggaca
     9661 cgtactttgg cagaatttat ggtatatgtc atcgaagaaa aagcaccatt tggtatttat
     9721 catttgtcga acgaaaatag ctgttcttgg tatgaattcg caaaagaaat attgaaagat
     9781 acagatgtag aagttgcacc tgttacgtca gaagaatttc cgcaaaaagc aacacgtccg
     9841 cagtattcag tcatgagcct gaaaaagaca gaagagttag gatttgtcat accaacttgg
     9901 caagaagcac ttgcgcaaat gctggaagct gccaaaaaaa tgaataagta atttcctttt
     9961 atcaaataga atttggagca tgatatggaa aaacaaacaa gaagtgcacg acgaaaagaa
    10021 aagccaaaag cgaaccaggc agattcgaat ttggtgcttg acctgtttct aaaactccgc
    10081 tatcttattg gcgttttagt catcgtggtc atcgttactt ttaatttaaa cggaagttca
    10141 cttggtgttt gggataaata tgtgtcgcaa agagacgatg ggaaaaaaac tgatgtgatt
    10201 tttggacaga atcgggaagt gcggtcagac gaatggctgg tgcagacacc tttttacctc
    10261 tcacaagctg aagaggactt tccgttggtg aacgaggatt attcattaaa cggacaaaac
    10321 atgatcattg cgtataattc tcctgtaaag gatattacgg ttatcggaaa accattcaac
    10381 tggggattct ttttcctcgg aagagatcga ggactttcct tttattgggc gatgaaatta
    10441 gtcgtcatgg tcttgcttgg attcgaagta ctgatgatat taacgaagag aaaaaaagct
    10501 ctatcgctaa tcggtgcatt tgcgctgaca ttttctccag cagtccagtg gtggttcatg
    10561 caacatgtgg gcgatttgat tttctttact ttgggactga tgactgcttt ttatcattat
    10621 ttctacaatc atgataaaaa atggctacga acgttgatga tgcttttggt ggttattttc
    10681 ggtattgggt ttattttagt gatttaccca gcccatcaag tgatgctggc gtacttgctg
    10741 attttttatt ttgttggcct gcttatttac tatagcaaga aagtttcatg ggatctgttg
    10801 gatgtagtgt tgattggagg ggcagtttta tttattggcg gagtcatggt tcatttctgg
    10861 ctaacgtcaa aagacgcatt gttagcttct ttgaatacct tatatccagg aaaacgggta
    10921 agtactggag gaaagtggac gattggcgaa tttttttctt tccttacaaa ttggaaaatt
    10981 ccttttgaag atatcaacta ttcgaataac tctgaagtgg ccctgttcta tcactttttt
    11041 ccaactgtat tcctagcaag tccatttgtg ttatttggca aaaaagatag cgaacaaaag
    11101 ttactgggac gtattttgat gattttttgt ctgttttcta tcttttggat taccgttggg
    11161 ttgccaaaat ggctagcaga aatcactctg ctgtcttatg ttcctccatt aagagccatc
    11221 cttactttta gttttgcggc ttgtttatta tcgatctggt ttatcgctta tatttggaga
    11281 gaagatgtta ttcctatttg gtataaattc attttgttgg cagtaaatgc atggtcttat
    11341 ttctatgcga ttacgcattc gaaaatggat aattattttt cggattttga aaccatcgca
    11401 gttgctgtgt tgggaacagt gattttagct tttgcattat ttggctggag acgcttgttt
    11461 tacctcgctt ttgccgcctt agttatcgta tctggctttt tcgtcaatcc agtcgtagaa
    11521 ggtacaggag cgatttttga aaaaacactg gcaaaagaaa tccaaaaaat cgatcaacaa
    11581 gatcctggta aagtttggtt gtctgaagat gaattatata acttcacccc aacattggga
    11641 gtgaaatcct tcaatacagt acgcttttat ccagatatag ctgcatggaa gaaaatcgat
    11701 ccgaaagaag aatacgagaa gtattataat cggtatgctc atacaagagc ttttatcgct
    11761 aaggaagata caaagttcat tttagatcgt ccagatatgt tttcggtcac gttgaatttt
    11821 gaagacgcag aaaaattagg gatcgactat gtgatcagta aacgaccatt aactgattac
    11881 aaccaattat atgaagcgca gttcacgcag ctatatggac cggataaaga cggttatatg
    11941 atttttaaag tagaatatcc ggattatcca gatgtaccac aaacacaaac tcaggaaaat
    12001 cagctagtcg tacagtaata ggctagctga gaataatcct gaaatgttag tagaaaggag
    12061 agtggagata attcaagcaa tcgtacaaaa atttgtcaac aaaatgagtg tcggtttctt
    12121 gcttttgtta ctgacgctag tcttttttgt ttggacagca gggttaggtt ttccagcaac
    12181 tttgtttata tataacaata gttcattact tatcgctttt agtgtggcta ttttgctcat
    12241 tttaaatgtt tataaagcaa aaggcgtcga ttttctttat ctaggtgttg cgcttgccct
    12301 tttcattttc ttttcttact ttcgggaatt aagaaatgag tctttatttg gagacttact
    12361 gtttcctttg attattttat tttgtttagt aattaaatgg atcgagtgga caaaatggga
    12421 tcgcggtatt tatctgattg tcttttcggt cttcctcgga atcacgttat atcgtgtatt
    12481 tacagagatc caagtaccag aaggacaaag catctgggct ccaggcaatt cgctttctga
    12541 tatttggatc aatacgaata taatcggaag ttctttgatg actcttgctt tattgatcag
    12601 tggatttgcc agttcttttg aacgatggta tgtacgaatc atcggtgcag tagcagttgt
    12661 tgcagctttt ctcgggattt gggtctgcca atctagaggt gcattagtcg cgttgattgt
    12721 ttttgcgata ttggatcttt tacctaaatc gttgatgcgt gtcattcgtg cgccatttat
    12781 tgcttacacg gttacgattc ttttagcttt gccgatttct tatctggcgg ctgtctctga
    12841 aaaagtcaat ttgtttaccg gtcgagaaga tatttggcat aaattctatc agacgctagg
    12901 agaaaaatct gaacaaattt tactaggaat gaaaacattt atcttccaac gaggaaacca
    12961 atttttagga aaccacaata gttataattc tattttgaat atctatggat tgataggatt
    13021 tgggattgct gctttgctgc ttatcctttt tattggtcgc ttaacgttga aagcagattt
    13081 atccaacggg cagatgactt ttatctgggc cttttttgcg gtgatgatgc agtcatttat
    13141 ggaggataca ttgacttcat acccatgggt tccgattatt tatatcctgt tggctatggc
    13201 cgttcatcga tatgatgaac cagaagttgc ggaggaattc tatgttatgg aagaggcata
    13261 tgacgatatg acttatgatg atacatctta tgaggaacaa ccagatagcg ctcattcttc
    13321 caggatccaa cgctatcatt gataggcaca aaatacgaga gaggctgtga caaccgtcgc
    13381 agcctttttt ggtttattgc aattgtctag catcttctgt aaaatagaaa cgttagagaa
    13441 aatcttgtat catagaaaat taagaaagaa ataagaacgc agctcggtac caaaaatatg
    13501 gtaaaatggc acttgactgt aattcactga aaatcgcttg gaggaacaca aatgtttaaa
    13561 gatttgtatt tatttatgaa ggacatttat cagaacagaa aattattgct acagttttcg
    13621 atcaatgact tcaaatcaag atatgcagga tcgtttttag gaatcttatg ggcgtttatc
    13681 aatccgctat ttactgtatt gatttattgg ctggtatttg gtgttggact gaaagcacaa
    13741 atgatcgatg caaaataccc atttatcgta tatttagtta ccgggatgat tccctggttc
    13801 ttcttctcag atgcatttgc atcaactacg gttgtttttc gggaatatac ttatttagta
    13861 aagaaagtcg tattcaatat tcgtatcttg cccacgacga aaatcctatc caatctgtac
    13921 acccacttat tcttcattct gattggcata gctgtcgcgc ttataaatgg tatatatcct
    13981 tcagcaatgt ctttacagct gatttattat ttattttgca tgtgtgcttt cttgacagga
    14041 ttgacttggc tgacggcatc gatccaacca tttttacctg atatcatgca attaattacg
    14101 atcgtgctgc agttgatcat gtggacatta ccgatattat ggagccctaa tcagtttgat
    14161 tcaggtcttg taagcatatt gaaattaaat ccgctgtact atgttgttca aggttatcgt
    14221 gagtcatttt taagtgaagc ttggttctgg gagcatggaa catatacttt gtatttctgg
    14281 gttttcacgt tagtaatgtt cgtaatcggc tctgcagtgt tccgtagatt gaaaccacat
    14341 ttttcagatg tgctgtaagg agaatagcta tgacagaata tgcaattaga ctaaaagata
    14401 tcacaaaatc ttataatatg tacgcaaaac cttcggatcg atttcgtgaa gccctcaatc
    14461 cttttaagaa atcctatcat gatgtatttt atgcgttgaa aaacgtgaat gtagaaataa
    14521 aaaaaggtga gatgatcggt ttcgtgggag aaaacggatc gggaaaatct acaatgttga
    14581 aaatcattac gggagtactg acaccaacat ccggcacgat ggaaatcgaa ggaaaaatct
    14641 cagctttgct agaactagga tcaggattca atcctgagta ttctggctat gaaaatatct
    14701 atttaaatgg aatggttcta ggattttcaa gagaagaagt cgatgaaatg gtcgatgata
    14761 tcattgaatt tgctgacatc ggagaccata tttatcaacc tgtaaaaact tattcaagcg
    14821 ggatgttcgt tcgtctagct tttgctgtag caatcaatgt gaatcccgat attttgattg
    14881 tggatgaagc attggcagta ggcgatcttg aatttcaact aaaatgtatg gaaaaattta
    14941 cagaaatcaa gaactcggga aagacgattt tattcgtttc tcatgatatc agttcgattc
    15001 gtcgtttttg tgatcgttcc tattggttga aaaacggaga agtcgtcgaa tatggcgata
    15061 cgatggaagt aacgacgaat tatgaaaact ttttaaagaa aaaatcagtt aaaacagtgg
    15121 atcggaataa aaaccaagta gaacggttcg ccgctccaga tatcgtcgac gtgacgaaag
    15181 cggaattgtt ggataaagac aaaaatccgg tggatatgct ggaacaagga gaaaaactat
    15241 acgttaaagt aacttatgaa gtaaaagatg atacaatcaa aaatccggtt ttaggaattg
    15301 cgctacgaac agtcgacaat ttttatgtat gcggacttaa tactctacta gatggtatca
    15361 agattccttg gaaaaaagga gagaatatct tttatctgga gtatgaaaag ttaggtttgt
    15421 tagctggtga atattatttt gacgttgcgg tctttgaaga aaatgcaacg gttcctttgg
    15481 tatataagac aaagtacatg aacttgtttg tcagcggttc ttatatcgga gaaggcatcg
    15541 tggtcttaga ccataaatgg gaagaaggga ctcatagcaa tgaaatatga ttttgaaatg
    15601 gacctagatg aacaaagcag tgtgggaaaa attgccgcac agatcaaacc aggaagtaaa
    15661 gttttagagt ttggtcccgg aaacggccga ttaacgaaac atttaatcgg agcaaaacaa
    15721 tgtgaagtga gcattgttga gttagataag gaattgttcg attttgtcag cgaatttgct
    15781 caagatggat tttatggtga tatcgaaagt tttgagtggg caaactatta tgctggacag
    15841 acatttgatt acgtcttgtt tgcggatgtt ttagaacatt tggtagatcc tggaaaaaca
    15901 ttgaagaaag tcagagaatt tttgaatgaa gaaggagaga tcctgattac attccctaac
    15961 ttggcgcata attctgtcat gatcgatttg ttcaataacc aattgccgtg ggcttcttat
    16021 ggtctcctag atgaaaccca taattctttt tatactcatg atggcttcca aaaagttttt
    16081 gaaaaagcgg gattattcat taatattgaa gattatttgt acttagctgt aggtgataca
    16141 gaattaaaat caacttatga agaattgccg gaagctgtac gctatgattt caaaatgcga
    16201 ccatttggtg aagtctacca atactttttc tcattgatga aacatccagt agcacaaagt
    16261 tccattgcag agccacaaaa ttctaattat gtaaaagtgt tagaagtaac ccagcaaacg
    16321 aaacaagatg aaacaatcca acaaatacca ttcaataatt tcacagggga aaatgagaca
    16381 ctaagtttcc ccgtatcaga aacaacagaa agaatggtgt ttcgcttcgc gcaacaacct
    16441 agctttatcg aattcagcgc agaagctgcc ggagaaaaac taacatttat cgattcgaat
    16501 gctgtagtca aaacagtaaa tgattgttat ttgtttgatg ggaaagaatt accagagttc
    16561 gttctaacgg atatttctgg gaaagaagtg actatccatt gtcactatcg ctttatcggg
    16621 gaattgacac caacaatgaa agaattgttg gaaacgattc gtcccatggc tgaagtcaca
    16681 aagcagcttt ctgagaaaaa tgacgaatta gaaagagaaa atcatcatct tcttgaggaa
    16741 aatacaagac tagaccatac gttaaaaaca accacaaatc gctattgcac attgttggaa
    16801 agtgatgaat ggacaataaa acatagacta ggacgacgta agaaagaaac ttcgaaaaaa
    16861 atccaagaaa aagaactttc tatatgtatt gacgagaaaa tctgggatgc agaaacaaaa
    16921 atattaaaaa tcatcggatg gggaatcgct aattcggatc gccagccctt gtcctataag
    16981 ttatcagctg accaatcgcc atttttcgaa gcaattcctg tttcacgaga agaagtcaat
    17041 caagcagaac agctcgcaaa aggaacagaa gcgggatttg agttacgaat cctttgcgag
    17101 caagaacgtt cattcttagt ggaagcagta gctcaaaatg gccaatcatg gatcattgaa
    17161 atgtaaataa tgattaactt ataaagataa ggagtaaacg aatcgtgcaa aaaaaagaaa
    17221 ttgccgtatt tatcgatcaa atcactcgtg aacgggccac tggcgatttg cttattgtgg
    17281 gctgggcaat tgatgaagtg accaaagaaa tccctacgat aaaggtagaa aaagaaaatg
    17341 tgattgctga agcaacacat gtcgtaagat tggatatcaa tcatttatat aatcttgatg
    17401 ttaagaccca aagcggtttt aaggtacgtt tatcagggaa aatgagagga aaagccatct
    17461 tagacttcca aacagcgaga catcaaaatg gcatagctgt gaaattaaac ggtaaatacc
    17521 cttatgatga cggcattgaa tcttcatggg aaagaaaaaa aagattactg aaaaaaggga
    17581 taaattatgc aaggactcat ggcgtaaaaa aagcgatccg tcgggtcaaa ttggaattga
    17641 agccaggatc gatcgactat gcagagtgga tcagtcgtca tgagaaacca gatttgaaag
    17701 agcaaaggaa agaagcacaa ggatttgcgt accgtccatt gatttccata cttatgcctg
    17761 tctataacgt tgaaatcaaa tggctcgaaa aatgcattga ttcggtactg gatcaaacgt
    17821 atgatcattg ggaactatgt atcagtgatg atgcttctac tgatccagct atccgaaagt
    17881 gtctggaatc ctatcaagca aaagatgata gaatcaaagt cgttttccga aaagaaaatg
    17941 gacacatcag tctggcaact aattctgcat tggaaatagc agaaggagaa tttatagcac
    18001 tcttagataa tgatgatgaa ttaccgccat ttgcattata tgaagtggca aaagtgctaa
    18061 acgtacatcc agaactggat gtgatctaca gtgacgaaga taaaatcgat gcagatggca
    18121 atcgctttga tccgcatttc aaagcagact ggtcgcctga tacattgatg ggaaataatt
    18181 atatctctca cttaggcgta tatcgaacaa gcattgttaa agaacttggc ggtttccgta
    18241 aaggctatga aggttctcaa gattatgatt tggtccttcg tgtgacagaa caaatccctg
    18301 cagatcatat ttaccacatt gaccgagtct tgtatcattg gcggacgatc ccaggatcaa
    18361 ctgcaagtaa tggcgaagcc aaaagttata tctatgattc cggagtgaaa gcattgacag
    18421 atgccctgtc tagaagaaac atcaaaggaa gcgtacgtcc tggcagaatt tctggatttt
    18481 atgagattgc ttatgacgtc ttgcaagagg atttggtcag tgtgatcatt ccaacgaaga
    18541 atggctatga agatctaaaa acttgtgtgg attcgatcat cgaaaaaacg tcttattcaa
    18601 attatgaaat cattattgcg gataacggaa gtacagatcc aaaaatgcaa gaattatttg
    18661 cagaatacaa gcatcagctg aaagaccgct ttatcgtaga attgattgac attccattca
    18721 attattctcg aattaacaat ttagcagcag aaaaagcaaa cggcaaatac tttttattct
    18781 tgaataacga tacagaagtg attgagccag attggatgac ggtgatggtt tcttatgcac
    18841 agtttgatcg aatcggctgt gtaggagcaa aactgtttta tccagatgat acaacgcagc
    18901 atgcaggagt attattagga atcggtggtg ttgcagggca tgcattgaat aactatgatc
    18961 gaacacattg tggttatttc ggccgattgg tcattgacgt caattactta gcagtcactg
    19021 cagcttgtat gatggtcaag gcagctgact tcaatgctgt taacgggttt gatgaaacat
    19081 tagaagtagc attcaacgat gttgacttgt gcttgaaagt ttatgaactg ggtcgttaca
    19141 atgtctatgc acatcaagca gaattgtacc atttcgaatc taaatcaaga ggttatgaag
    19201 atactccaga aaaacaaaga cgttttgctg gtgaaatcaa aaaaatgcaa gataaatggc
    19261 cagcatatat tgcgcatgat ccattttaca atgataattt aacaaaagaa ggaatcggcg
    19321 acttttcgtt gagaccagat taaactataa taaaaagctg ttatgttgtg attcgctcgc
    19381 acataacggc tttttatagt tttttgtcaa aaaaaggcag aaggtgccga ctcaaaaaaa
    19441 taagtcgagg ttgggacaga agtgtttaac tccgagaaat aagaagaaat tcacgaaaat
    19501 tgcttttcaa atttttgtga atttcggctt atttaagaag aagttgcttc tgctcccacc
    19561 gtttattcgt ttttaaagaa tgagacaaaa gtgtttctta cttttgtccc acgctcttat
    19621 tcatgctcag agcgccaata gatacaaaat catagccaaa gtttcacgaa ccctagctgt
    19681 caaagaatcg atattttatt ttaatatagg ctattttaaa ataaatggtt taaggtattc
    19741 ctctacaaaa gccactctat caattatcat gggtttaaaa taagaagtga tggagataac
    19801 cagattgttc ttagcgtcaa tataaagaac atttccacta ttaccaattg ctgaatatat
    19861 ttcgttctca gaatctaata cccaccataa atagccatat ctcatatttt gtaagtcttc
    19921 taaagcactg gttctaggtt tgaacatctc gtttatccac atgggtggta cgatttgttt
    19981 tcctttatac atccctttat ttaggcataa tacaccaatt tttgccagat cacttgccta
    20041 taaacacaaa ccatatcctg ccgtcccgat atttagtgga tcacaaaacc agacattggt
    20101 tttaggagac ttatccattg taaactgctt atgttcttca gctgtttcag cataataatt
    20161 gacatggttt ggtatcccta taggatcaaa tagatactta ttggcaaaat caacagttgt
    20221 tagcttactt gtctgataca acaagccagt taatatgtgt aaacagacag tttgatagtt
    20281 gaattcgccg gttatacctt ttcttcctcc caataaatct agtgatgttt ttgtccaatc
    20341 ttcacttgaa cagacttttg tccatggatc gcctttacat ttgtagggtg ctttcattgt
    20401 tagcaagtgc ttgattgtaa tagcgtaaat agttttctcc ccgcgtttca ctttatactc
    20461 tggaaaataa tctaatactt tatcatcgat acttttgatt aatccttgct ctaaagcaat
    20521 gccaatcagc aaagatacaa tacttttcgt tgctgacatg atatgacaag tatcgtcttt
    20581 tttataacca ttccattcat ctgtataaac ttctacccca tccttatatg cgacaatttg
    20641 acatatattt ggttgtcgaa aaccaataaa atcatgcaac tcttgtttat tcataagata
    20701 ttacctcact ttcttctata agcataatgt cattaaaaat cgatttcaat tttttcgatt
    20761 cttcacgtaa tacaggtatt cttatggact agacagcgct ttgggaagat ggattctctg
    20821 aaaaatataa aggttcactt tatgagtgga taaaaaaagc agcaaatact tcgacttctt
    20881 taatttacca acattagtga aaatcgattc atctggagat ttgtaattta gagtccttgt
    20941 cttgattaga ggtgtataaa gctttttatt agacattttt aaattccatt acaatttgct
    21001 tataaactag gtttttaaag gtgactatgg taagataata gagatattat tttgtgatta
    21061 ggagtgtata tatgaataaa aatggagaat ggagtgctgc acgacggata ttcattatat
    21121 taatggatgt tattgtatac aatgcagcaa tatatagtag tttcttactg aagtttcgtg
    21181 gagagatccc attacgaaat ttagaaactt tccaacactc agcaattatt atttcagtga
    21241 tttttattgc gttgaacatt ttgttgggtg catatgtttt ttataatcga atgatcagtg
    21301 acatcgtgtt tgtcacagtc atcggtcagg tattgatgac actagggatc atggtagtga
    21361 cttttgcggg ccgctggttt gcttttccac gtcttgttat cttactttca tttgttctcg
    21421 gtacgctttt cttaagtatc tatcgtattc tcgtatataa actttattta cgcgtaagtc
    21481 gagataaaaa ggtcgtcatt attgggttag aaaaggatgt cgcaccagca atccaaaatt
    21541 ttggttcaaa gaaaaacaac aaacataaag tcgaagcagt cattcttgat aactattatg
    21601 caaacatcaa aaatatgatt aaagacatcg atattgtcta tcttgcttct catatcgaag
    21661 aaactgaaaa aatcaaaatc tatcaattag ttactaaaaa tgaaaaaaaa ttgtttttga
    21721 atacaacatt tgaaaacttg acattgatca atccaaatat tatgaatttc gaagatgaaa
    21781 gtattattga agcatctgga tttagaatcc aacctgaata tgaactattc aaacgtttgt
    21841 ttgatatcat cgtttcactt gttttattag ttattgcttc accatttatg ttattcacag
    21901 caattttagt caaagtgaca tcaccgggac caatcatcta taaacaagtg cggatcacaa
    21961 aaaatcaaaa ggaattttca atctataaat tccgctcgat gaccgccaca gccgaagcaa
    22021 aatctggacc agttttagct aagagcaatg atgcacgagt aacgcctgta ggtaaattta
    22081 ttcgagctgt ccgctttgat gaattaccac aaatcttcaa tgtgctgaaa ggcgatatgt
    22141 cgatcgtagg accacgtcct gaacgtcctt tcttcgtcga tcagttcaat gcagaaaacc
    22201 cttattacta cttacggcat aatgttcgtg ctggtatcac tgggtatgca caagtctatg
    22261 gaaaatatgt ctctgattac aacagtaaat tacgttttga tttactttat atcaaaaaat
    22321 attcattgat gcttgattta aagatcttgc tgcaaaccat caagatccta tttgataaaa
    22381 tgtcctctca aggacttgaa gaagacgaaa atgtgatctc attggcagaa attgcttttc
    22441 cgaatgaaaa aatctttcgc tagttgggag gcgtaaaaat gaacaatccg aaattcagca
    22501 taatcgtacc agtatacaat gcagaagcaa ctattggtcg aacgattgat attctaaaag
    22561 aattggatta tgaagagtac gaagtcgttt tagtaaatga tggatcaaca gatcaaacac
    22621 aggaaatcat tcaagaactg attgaaaatg atgaacgttt tgaattgatt actacgacca
    22681 atcaagggcc cggattagca agaaacgaag gaataagaca gtcaaaagga gaatatctct
    22741 tattcttcga tgcagatgac acgccagtaa aaacgatctt gtcagattat gatcatttgc
    22801 tagaaaagag tccaaaagcg gatcttgttg ttagttcgtt tgtttttcgt acaatggacg
    22861 agggaagaat cgtttccgaa aaagaaaact tagttacaga atgtcactat ctaactcacg
    22921 aagagtttat aaaagacatg tatcagttaa tgaatgaaca gttgatgtat gttgtatgga
    22981 acaaatgcta tcgtagcgat attctgaaaa ataaccaaat tctatttaaa acttataata
    23041 gttgtgagga tcgaatattt aacttacatt attatgaata ctgtcatcaa gtgatcatga
    23101 acccaaaagt agaatatatt tatgaatttg aaggtgggaa ggggatcact aatcaatacc
    23161 gacctaacaa gttcgaaaca tttaaagaat tctattcatt agcaaatact gtaacagacg
    23221 atattaataa agacggtatg gcggcgcttc ttttaaaagg gacaacttca gttatttttt
    23281 ctatttatag tacctctgca agaacttcaa aagagaagaa agacgaagca aagcagatat
    23341 tgagtgatcc agtgatccga gaagccaagc aaattgctaa gacggattct tcagcaaaaa
    23401 aaataacaaa gttgctttac aatatgccag ctccactatt tttatctgct gtaaaagttg
    23461 gaagcttcgt tgaagtgaaa atgccagggt taatggctgc tttgaagcga gtatattagt
    23521 taatggagga aaatataatg aaaagaattt tgcattttca gggacgaatg ggtttaggtg
    23581 gagcagagtc tttcatgatg aatttatatc gtaaaattga tcgtacaaac tatcaatttg
    23641 attttttgat ttatgaagat tatgcagatg ttcaagacta tcattcagag attgaaagat
    23701 taggaggccg tatttttgtt gtacctaatc ctaagaaaaa tatttttaaa tatttaatag
    23761 aagtgaataa gttactaaaa aaagaatctt tttctatagt tcacaatcag gtttattttg
    23821 gcgggggaat taatttatgg ttagccaaaa agaatggaat cagacagaga attgctcata
    23881 gccatgcaac tgaagatgga aaatctcaaa atattgtaat gaatgtattg cgtaaatttt
    23941 taaccaaatt gttgttacaa aatgcaacag actaccttgc cgttagtcaa gaagctggtg
    24001 aaagtctgtt tcaaaaccac ccctttgaga tagttcataa tggtattgat ttagagcttt
    24061 atagtaaaaa ttcagaagct aaggttaata agagaaaaga attggatatc tcaatgagta
    24121 cttttgttgt gggaaacatt ggacgattag agaaacaaaa aaatcaatct tatttgttag
    24181 agattttttt agaaatacta aaaatgaatt ctgattctta cttgatttta gtaggtgaag
    24241 ggtcacttag aaataaactg aaagccaaag cagtagaatt ggatatagag aaaagagtaa
    24301 aatttttagg agaaagaaac gatattcccg agttattatc tgtgatggat gtttttgtaa
    24361 tgccttctct ttatgaaggt ctgccaatgg ttggattaga agcacaagca gcaaaattaa
    24421 aattagtctt agcagatact atttccaaag atacaaagtt aacaaataat gtggattttg
    24481 tatcattaga tgaaacacca caagaatggg caaaaaaagt aataaaagag ccgtatgcta
    24541 ataaaataac acaagaacta aagagttatg atgtttcgta cactact
//
LOCUS       DAYYGO010000030        24247 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000030 DAYYGO010000000
VERSION     DAYYGO010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.030"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(44..1339)
                     /gene="purB"
                     /locus_tag="ACHA5T_001021"
     CDS             complement(44..1339)
                     /gene="purB"
                     /locus_tag="ACHA5T_001021"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFM8886612.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1352..2476)
                     /gene="purK"
                     /locus_tag="ACHA5T_001022"
     CDS             complement(1352..2476)
                     /gene="purK"
                     /locus_tag="ACHA5T_001022"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFM8886613.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2469..2957)
                     /gene="purE"
                     /locus_tag="ACHA5T_001023"
     CDS             complement(2469..2957)
                     /gene="purE"
                     /locus_tag="ACHA5T_001023"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFM8886614.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3373..4680)
                     /locus_tag="ACHA5T_001024"
     CDS             complement(3373..4680)
                     /locus_tag="ACHA5T_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFM8886615.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4683..5264)
                     /locus_tag="ACHA5T_001025"
     CDS             complement(4683..5264)
                     /locus_tag="ACHA5T_001025"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFM8886616.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5380..5477)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5836..7128)
                     /locus_tag="ACHA5T_001026"
     CDS             complement(5836..7128)
                     /locus_tag="ACHA5T_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8886617.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7125..7748)
                     /locus_tag="ACHA5T_001027"
     CDS             complement(7125..7748)
                     /locus_tag="ACHA5T_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886618.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7751..8587)
                     /locus_tag="ACHA5T_001028"
     CDS             complement(7751..8587)
                     /locus_tag="ACHA5T_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8886619.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8604..9491)
                     /locus_tag="ACHA5T_001029"
     CDS             complement(8604..9491)
                     /locus_tag="ACHA5T_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8886620.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9507..10790)
                     /locus_tag="ACHA5T_001030"
     CDS             complement(9507..10790)
                     /locus_tag="ACHA5T_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8886621.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11473..12156)
                     /locus_tag="ACHA5T_001031"
     CDS             complement(11473..12156)
                     /locus_tag="ACHA5T_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFM8886622.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12174..12791)
                     /locus_tag="ACHA5T_001032"
     CDS             complement(12174..12791)
                     /locus_tag="ACHA5T_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFM8886623.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12801..13625)
                     /locus_tag="ACHA5T_001033"
     CDS             complement(12801..13625)
                     /locus_tag="ACHA5T_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFM8886624.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13618..14178)
                     /locus_tag="ACHA5T_001034"
     CDS             complement(13618..14178)
                     /locus_tag="ACHA5T_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886625.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14352..15308
                     /locus_tag="ACHA5T_001035"
     CDS             14352..15308
                     /locus_tag="ACHA5T_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8886626.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15613..16569)
                     /locus_tag="ACHA5T_001036"
     CDS             complement(15613..16569)
                     /locus_tag="ACHA5T_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFM8886627.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16673..17275
                     /locus_tag="ACHA5T_001037"
     CDS             16673..17275
                     /locus_tag="ACHA5T_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM8886628.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17363..18862)
                     /locus_tag="ACHA5T_001038"
     CDS             complement(17363..18862)
                     /locus_tag="ACHA5T_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFM8886629.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18878..19402)
                     /locus_tag="ACHA5T_001039"
     CDS             complement(18878..19402)
                     /locus_tag="ACHA5T_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFM8886630.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19700..20449
                     /locus_tag="ACHA5T_001040"
     CDS             19700..20449
                     /locus_tag="ACHA5T_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM8886631.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20440..21225
                     /locus_tag="ACHA5T_001041"
     CDS             20440..21225
                     /locus_tag="ACHA5T_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8886632.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21194..21910
                     /locus_tag="ACHA5T_001042"
     CDS             21194..21910
                     /locus_tag="ACHA5T_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8886633.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22012..23031)
                     /locus_tag="ACHA5T_001043"
     CDS             complement(22012..23031)
                     /locus_tag="ACHA5T_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFM8886634.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(23050..24051)
                     /locus_tag="ACHA5T_001044"
     CDS             complement(23050..24051)
                     /locus_tag="ACHA5T_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFM8886635.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQPMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
BASE COUNT         7184 a         5222 c         4223 g         7618 t
ORIGIN      
        1 ttttgctatt ctattttaat tgaattttgc aactcactct tctttattcc aaacctactc
       61 gtttgaatat cacgtcaacg tttttcaaat gatgatgata atcaaaggca tcgtctattt
      121 cttctttaga taataccgat gtgatggttg gatcttcttc taacagcggg cggaactgca
      181 cttggttgtc ccaagcgtac gcagtttttg gctgaacaag atcataagct tcttctcttg
      241 tcattccctt atcgatcaat ttcagcataa cgcgctggct ataaatcaaa ccaaatgttg
      301 cagacatatt ccgcttcatg ttttcaggaa agaccgttag atttttaacg atattgccaa
      361 aacgatttaa catgtaattc aataaaatcg tggtatcagg caagatgatt cgttctgctg
      421 aagaatgaga aatatctctt tcatgccata atgtgacatt ttcataggca gtgaccatgt
      481 gtccgcgcac cactctggct agaccagcca tattttctga accgatcgga ttccttttgt
      541 gcggcatagc agaagaacct ttttgacctt tcgcaaaaaa ttcttctact tcacgtgttt
      601 cggatttttg caatccgcgt atctctgttg caaaacgttc aatacttgta gcaataagcg
      661 ccatcgctga gaaatattca gcatgtagat cccgagggag aacttgagta gagatttctt
      721 gtggacggat ccccaattgt ttacatacat actcttcaat aaatggcggg atatttgcga
      781 acgttcccac tgctcctgag atttttcctg cttctactcc ttttgccgca tgttcaaatc
      841 gttcgatgtt ccgtttcatt tctgaatacc atgtggcaag tttcaatcca aaagtagttg
      901 gttcagcatg gacaccgtgt gtccgaccca tcatcactgt atatttgtgt tcttttgctt
      961 tttcgccaat gatttccgta aaacgtttca agtcttcccg taaaatatca tttgcttgct
     1021 tgatgagata gccataagca gtatctacga catccgtact tgttagacca taatggaccc
     1081 attttctttc ttctcccaat gtttcagaaa ccgctcgtgt aaacgcaacg acatcatggc
     1141 gtgttaattt ttcgatttcc aagatccgat cgatatcaaa tgatgcatgc tcacgaattt
     1201 ttttcacatc ttcttgcggg atttcaccaa gttctgccca agcttcatct gctaaaatct
     1261 cgacttcgag ccacgcacgg tatttgttct cttccgtcca gatattcccc atttcagggc
     1321 gtgtataacg ttctaacatg ttttttctcc tctaatccca aatattcgtt tgtttgattt
     1381 cttccagcgt ttgctggatg tcttctgtca agatagtgat atgtcccatc ttgcgacctt
     1441 tttttgcttg tttttttcca tagaaatgga attgccagtc tggcttattc tcaatcaagc
     1501 gatagctttc cagcagttgt tctcccaaaa cattgaccat tactgcattg cttatcaaac
     1561 gtactttttc tcctaatggc caaccacaaa tacctcgaat atgggcatcg aactgactca
     1621 tagaacaagc ttcaatagaa taatgtccag aattgtgagg acgaggagct aattcattca
     1681 catatagccc gcctgttttt gttaaaaaca tttctacccc taaaacacct gaaaggctca
     1741 ctgcttctgc aatcacacgg gcaatacgct cagcctcctc gatcacatcc tgatcaatcg
     1801 cggctggagc aatcgtttca tgaagaatat tgttgtggtg gatgttttcc acgaccggaa
     1861 aagtcgtgaa atctccttga ccatttcctg ccaccatgat cgaaatctct ttttcaaacg
     1921 ggatccatgc ttctaaaaca caggttcctt ctcttaaaag gtccatggca ggagctagat
     1981 ccgcccgact tttcaaaaca tactgcccct tgccatcata gccgcctcga gtggttttca
     2041 aaacacaagg atagccgatc ccgtcaatcg catcttggat atccgtcgga ctgacgatcg
     2101 ttgcataagg agcaatcacg atgttattcg tttccaaaaa agatttttct aacaggcggt
     2161 cttgcgtaat cgcaagcaag tctgtccctt gaggaataaa tgacatgggt aaaatcgcat
     2221 ttaacgcctc tacactgacg ttttcgaatt cataggtaat gacatctgtt cttctggcaa
     2281 gctctttcag cgcaaaggtg tcatcataga cactttcaat gtgccaatct gcaacttgtg
     2341 cggctggaca atcgctcaca ggatccagca cacctacttt aaagcccatt tcttttgcac
     2401 taatcgccat catacgacct aattgaccgc caccgacgat cccgatcgtt gaaccaggca
     2461 acaaaggttt aaccaagttg atcactactt tctataacag ttttagccaa cattgaacgt
     2521 ctttcagcca atttagcctc caattctaaa tcatacatgg aaagcatctg tatcgcaagt
     2581 agtccagcat taattgctcc cgcttttccg attgctgtag tagctacagg tactcctcca
     2641 ggcatttgga caatcgaaag aagcgaatcc aaaccattta atgttcgtga ttggaccggt
     2701 acaccgataa ccggcaaagt tgtttttgat gcgaccatac ccggcagatg cgcggctccc
     2761 cctgctcctg caataatgat tttgattcct ttttttcgag cgttttgtgc atattcaaac
     2821 atcaaatctg gggttcgatg agcagaaacg accttttttt cataagacac accgaattca
     2881 tccaatattt gacatgcttc tttcattgtt tcccaatctg aagtactccc cataatcaca
     2941 gagatttttg ctgtcattgt ttttcctccc agtttttcta cattcatatt ttaacaatca
     3001 ctttttttga tgtcaaagaa atttcgaatg ttaacaaaga aaaacatttt attgttcgtg
     3061 tttattaaag ataaaaaaaa ggaatgtgac aaaagttagg tcacattcct ttttatggat
     3121 aaacggtgtt ctcctgccaa ctcctcggtc acaaggaaca atattcaaca aacatgagaa
     3181 gctttttttt gaaattgttc cttgtgactc ggagatgaac ggcaggctca cgacctcttc
     3241 cttcaacggt gttcaatcag ctgaccctcg gtattagctc aaacaaacgg caaaatacga
     3301 ggaactattt ttccgtttgt ttctctaaca atcaggtcaa aacgctgatt tcacaacctt
     3361 tcttttgctc tattacttct tgaaggcttc ttccaatatt tcttcatctt ttttcaatcc
     3421 attaaagatc aaattcaata tgatagccgt aatgctactc attacgatgc catttccagt
     3481 gaacatttgc aatgtttgcg gcatttgact gaataatgtc ggcgtaacat tgaaacccag
     3541 cccaaatcca acagaaacag caacaatcaa tagattttta tcattgctga aatcaacttc
     3601 caacagcata cggattcctt ggactgccac catcccgaac atgactaaca ttcctccacc
     3661 aagtaccggc tcagggatga tttgtgctaa cgcaccgatt ttagggaata atcctaaaac
     3721 aatcaagaag aatgcagaga aatagatggg ttttcttgtt ttgattcccg aaagttgaac
     3781 caatccgaca ttttgagaaa agccagtata agggaatgta ttgaaaatac cgcctaagat
     3841 aacggcaagt ccttctgcac gatatccctt tttcaattct tcttctccaa catttttccc
     3901 agtaatgtca ccaagtgcaa aatatacacc tgttgattct accatgctga ccatggaaat
     3961 gatgatcatt agtaaaatcg accatacgtc gaaagttggt ttaccaaaat aaaagggttg
     4021 tggaacatgg aacaacggcg catctccaat cgcgctcaga tcaatcattc ccatgaaagc
     4081 agctaagatg cttcctccga ctaaaccaat caataccgcg attgaacgaa tgaacccttt
     4141 tgccagcatt tgcacgccta caattaggag aatcgtcaca aatgcaagta gcagatttgt
     4201 tttgtcacca aaatcagctg ctgttgcatt acctccaccc attttctcga ttgctacagg
     4261 aatcaaagtc aaaccaataa cggtgataac cgttcctgtt actataggag ggaatagttt
     4321 tttaatttta gaaaagacac cagaaactaa cacaacaaag atcccagaag cgataataga
     4381 tccgtagata gctcctactc cttcattggt tccgatcaag atcagtggag ctactgcttg
     4441 gatcgcacat cctaaaacga caggcaagcc aattccgaaa aatcggttaa ctgtcaattg
     4501 caataatgtc gctaatccac acataaagat atcaattgaa atcaaataag tcatctgttc
     4561 ttgattaaag cctagtcctg taccgatcaa aagaggcacc gctactgctc ctgcatacat
     4621 cgctagcaga tgctgcagcc ctaaaaccgc agcttttcca ttttgtgttt ctttttccac
     4681 gattatgcgt cctcctcaag aaactcaact tgtccattcg tcaaggaagc aatacgcgca
     4741 agagaaacga ctctcaatcc catttcttca agtaactgac gtccatcttg gaaagacttt
     4801 tcgatgacga tcccgattcc ttctacttgc gctccagctt gctggcataa ttcgatcaat
     4861 ccttttgccg cttgtccgtt tgccaagaaa tcgtcgataa tcaatacttt gtcttctgaa
     4921 gataaaaatt tacgagaaat agaaatcgta cttgtcactt gtttcgtaaa agaataaaca
     4981 gaagctgtca ataattcttc atccatcgtc aaactttttg cttttctagc gaagatcatt
     5041 ggaacatcta attctcttgc agcaaataaa gctggtgcga tcccagaagc ttcaatggtc
     5101 acaactttcg tgattccttg ttctttaaat acttctgcaa accgttttcc catttgttcc
     5161 atcaatacag ggtctacttg gtgagtgacg aagctgtcga cttttaatac gccctcgcct
     5221 aatatacgtc cgtctttttg aatacgttca actaattctt tcactttatt tgctccttta
     5281 tcatttatta ataccgaaat aaccgctttt gtgcaaaaac ctactatgtt tctgttaaag
     5341 aaaaacaccc tccactttat cataagaaaa gaagggtgaa atagccaatc ttttacttat
     5401 agtccagcat ttacggtact ggctagaaac tgtcatccat attatgacga tatataagta
     5461 ggtatgtaaa actaataata gtgtaaattc attttcttgc aactgttttg cacgtgaagg
     5521 cagtttgcca tatttgaata aaaaatgttg tttcaaatat tgaaaacagt ctaacactac
     5581 tttctgaaac tgacaacgtg gtttacttat ttttttgtaa aatctaatga tttttaatgc
     5641 attattcctt tttgttcggg tttaaatagt tatttttatt ttctcctatc taaaaaagca
     5701 gcccacataa aaagaaggca tgacaaaaag catgtcatag cccctttttc cgaataaata
     5761 gtgttcaaaa gacagcctat cggcaataag gcaagatttt ataaactacg ggaagtaatt
     5821 tccgtaaatc tattcttatt cttttaagct gacaagctcg tcaatcacta ctgcttttcc
     5881 tgaagcaata gaatgatttg ctgcgattcc tgtcaaaatc gacaaaatac catctacgtg
     5941 agttgctgcc cgattccacg gatcttcttc tttttcttca ccaaatagat catctaataa
     6001 acgcggatct cctccgccat gtccgcccgc ttcttgttcg acgattacct catatggctc
     6061 accgaacatc ggatagacat ggatggactg aagaacggct gctccttcat cctttttatc
     6121 tccacctgca ttgatgtaag acttttcgat cactttcatt tcgatccgtc ctttgctgcc
     6181 attgaaagaa acattgaatc cttcccacgg catatacgcg tttagcgaat acgtcaaaat
     6241 cgcaccagtt tcatatttga ccgttacgcc tagtgtatct tcaatagaga tatcatcgga
     6301 aaatacactg cggtcacgaa tatatccact gtctttttct gcttctaaat acatttctcg
     6361 caattcctca ttggctgaca ggtctatagc aaatggatca gctttagcta gttcgcttcc
     6421 atagcatcga ctgtaaaata tttctactcc gcgtttctcc gcattatgca tcccataaaa
     6481 gtttaatcca ccgaatgcaa ataccttttg gggacggctg ttcaaccaaa aattcactag
     6541 atcaaaatga tgggtagatt tatgaacaag aagcccgccg ctattgtgct tatttcgatg
     6601 ccatcttctg aaataatctg ccccgtgttg cgtatttaac agccattcga agtggataga
     6661 aaacacatca ccaatcacat cactttctat cagttccttt atcttcgtgt gatacggtgc
     6721 ataacgataa ttaaaagtga ctcgcaggct tcttcccgtc tgtttttgga ccgttattat
     6781 ttcttgagct ttttcctcat cgatagtcag aggtttttcg caaataacat catatccttt
     6841 tttcattgca cgaatgatat aatcatgatg cgttcgatct actgacgtaa taatgacttc
     6901 agccggccgt acgtcatcca gtagttgctc aaattgtgat ggcatacatg tctttacttt
     6961 tttctgtcca tacttgttca caagtatccg atttgcataa tccatccttg tttgagatac
     7021 atcacaaaaa ccgactagtt cacaagtttg atgatacgta gaggcaattg cctcataaaa
     7081 aaaacgcgct ctcccaccaa gtccgacttg tacatatttt ttcttcattt aaagtcattc
     7141 tccttttctt ttaagcaaaa tactgtgaca aatggcagcc ctggatacag taatgcagtg
     7201 agtaaaaaat catgctgtat acaccagaac agtccataca aaaggcataa ataccacaga
     7261 aaccccttga cgttttttga caacagccag atattccttt tgatcaaata aacaagacta
     7321 tctgctttat cttctttgca gatatcaatt agcgtactaa aaaaactatg ggatacaaaa
     7381 agcaacagac acaatagaaa aggaatcaga taccaagctc caatctttcc cactaataaa
     7441 taccaatcaa ctgcaaaaat aatgacaaac agctgaaaca acgtaatgat gaaacacatc
     7501 cgtttgattt ttcctttcct ccaacatacc tttttttctg ccaagtaatg gacagataga
     7561 taataagaag gatacatcat caaaaaataa atcagataga caacacgagc agacagcact
     7621 aatcctaaag tgaacaaaca tcctagaact aaagcgaaaa gagcattact gataagactg
     7681 gttaaaaaaa tccgacagac atagtagttg agcttgtaaa gaaacatctc ttctattggc
     7741 tttttcatat tcagcccttc agaccgcttg tactgatccc ctggacaaga tatttattga
     7801 agaataagaa aatcagaaaa accggtagca gcgacaacgt agacatcgca aacatactcg
     7861 cgtaagtcgt actagatcca gcgtctgcaa ataatttgat ggcaatcgat accgtgtaag
     7921 atctcggctt gtttaaataa atcaatggtc ccatgaagtc gtcccatttc caataaaact
     7981 ggatgatgat cgtcgtgatg atcgaaggtt tcagcaaagg aaaaataatt ttggtaaaga
     8041 cactatactt gctgcatccg tcaataatcg ctgattcatc taactctttg gggatgctga
     8101 ccatgaattg catgatctgg taaatgaaaa atgcttgtcc aaaaaaatgt ggtaaaatca
     8161 gcggcacata cgtcccaacc aaattcagcc gattgtaaat aataaactgc ggaatcaaaa
     8221 tcacttgtgc aggaatcatc atcgttacta acatgacgac gaaccagaat ctttttcctt
     8281 tgaaatcgat tctcgataat gcgtaagcaa tacaagtcga gctcagaact gtccctatcg
     8341 tcgaaactgc agagatgaca aacgaattcc taaagaaagt aaaaaatgaa attccagcaa
     8401 accccgccca tccttttgcg taattttccc attgaaaagt ctctggaatc aaattcagcg
     8461 aaccactcat aatctcttga tccgttttaa aagaacctgc gatcatccat aagaccggat
     8521 aaatcatgac tagacctaat agcaaaataa aaccgtattg aaaccatttc gttactgtat
     8581 tattcatttc ttcctcctct cattcacaca acgacccatt tttttgatga tttgaaaatc
     8641 agaaaggtga cagctcccat aatcaacagc atgatccacg aaatagcact agcatacccc
     8701 atagaattaa aagaaaaagc ttggttatac acataaagag cataaaagtt cgtggcatca
     8761 ttcggaccac cttgggtaat gataaatgcc tgcgtaaaag tcataaacgc cgcgattgtc
     8821 tgcataacca agttatacaa aatgatcggc gaaagaagag gtaacgtgat cttgtaaaac
     8881 gtctgccatt tgttcgctcc atcgatctct gccgcttcat aatacgagcc tgggatctct
     8941 ttgatacctg cagcaaagat gatcattgcc gaaccgaatt gccaagcaga catcaaaaca
     9001 agagggatca tgaccagttt ttgatcgcta aaccaggaaa gcgcaggtgc gcctaatcgt
     9061 tccaggaaaa tattcactaa cccgttttta gaaaacaact cccgccaaac tagagcgata
     9121 gaaatactgc caccgatcaa actaggcaca taatacaaag aacgaaagaa agtcgttcct
     9181 ttgatattcc tcgttaaaag ataagcaatc aatagaccaa agatcagttt cacaggtaca
     9241 gatgcaaata catagagaaa agtgactcgg atggagttca aaaaacgggc atcactcagc
     9301 atctttatat aattatccaa gccgatccat gagactggat ttcccaaacg ataatcggtg
     9361 aacgaataat aaagtgacaa gatcatcggt atcactgtta tcaatagaaa tcctaggatg
     9421 aatggagaag caaatacgta accgaccaca ttgtcttgat ccaagaaccg ttttagtttt
     9481 tcttttttca tgccttcctc ctgctgctat cctagaatgt ttgacgcaaa ctcataaaga
     9541 ctctctgctg cttcttctgc tgttacttct tcataaatga ctttttcaac taaaagattg
     9601 tattggtctt gaatttccgg attcaaagca ctatcaaaga cattcacttc gccgttgtcc
     9661 atatcggaaa tcatttctaa ataatcaaat gtcgcttgca aagttgcatc atctgcacga
     9721 gcgagctgtt ctcgaatatt cgacatgatt ggtacaccac gttctccttt taaaatttca
     9781 ttggcttctt cactgttttg aaagaaatca ataaacttag cagcttcttc tttatgggct
     9841 gagctttttg ccatactcag catttgcgaa gaacgtaaag ggataccaga ttctccatct
     9901 gcttttctgc gaggtggatt aatcaactga atctccatat cttcaggagc ccctgacaat
     9961 atttctgtca actggttact tgatagaaac accattgccg cttctctatc cctaaccgcc
    10021 tgaaaagaca gatccgtcgt acttgcagct ccaccacctg gttctggatg ggcccctttg
    10081 tctaccagat ttttacgcag ctggaaaaaa tctgttagat agtctggatt ttcaaatccc
    10141 aagctgttag atagatcttc ccgattataa aaattcaatc ctttctcaac ttgtggtatt
    10201 tgaataatac agccagctat aaaatcttcg aatcttgaaa tagcatagat tcctaattct
    10261 tcagtgattt gatagctgat ttgttcaaat tcatcccatg tccaatttga agctggttca
    10321 gggatattta attgttcaaa tatattttta ttgtaagcaa tcgcaaacga attcaccccg
    10381 ttagacagtc caactaattg cccgtcgtaa gtcgtcgttg ctaaatagga ttcatctgca
    10441 ttagaaatat caattatccc ttgctccaca tattcatcta atggttcaat ctgattgatg
    10501 tattgaggaa attgattgtt cagttggaaa atgtcccaga tatcatctga tgctgctagt
    10561 gtacctaatt ttgtgtaata tccttcataa tcataaaatt cataatcgat cgtcacatgg
    10621 ggattttttt cttcataaag ctcgattact tttactgttg catcgtgtcg gacttgcgaa
    10681 ccccaccaag ccatgttcag cgtgattttt ttgtcatctt cttgcccaca agccgtcaag
    10741 aagaataaca taacacttaa agctaagatg attctcattc ttttcttcat tctctgcacc
    10801 tctacttcca ttgttatttt tcaatatact gtatttttat acagaacata tttattaaac
    10861 gatctttttc aaggggaata aaaaccaaga cttgccttat cgttctgcct aggatgaaca
    10921 actcaatact ttcaagaaaa attagatagt gctgattctt ctttcatcgc taaaaacact
    10981 tcttgcttca atgatgggag gataattacg caaagaaaaa aggatatcag acaactgccc
    11041 aatatccttg acatagctaa ataagcatgc ttcttcgtgt gcgctcttcc ctaaaaaaca
    11101 gaaaaataac tagtaattct gtatgaaatt aggtacaata tacacgtcga tgcacaagca
    11161 ttgactatgg caacttgatt ggtcagttcc cgctgacttt tcaagccccg acaatttagc
    11221 tgctactaaa ttgtcgggtg ccttttttag tttttattcg tttttcattc tatagaaagc
    11281 gcttcaataa atcaagacat ttttttgtta gaatgctaat aataacaaaa ggttttaact
    11341 cttcttatgt acattacagg taaaaaaaca aaaaaatttc tctttttttt gaattctgaa
    11401 caaatagtac tggagtacaa aagacgagga agagccacta tctgctcttc ctcgtctttt
    11461 gattctcacc tgttattcat gattcttttt ctcagtttcc cgatattttt ccattttacg
    11521 actaaaaagc tctccgctac ttggtggcgt atatgtgatg gcatttgctc ctgcttgaat
    11581 ggtctcagca atactttctt ccgttggacc acctgtcgca atgataggaa gttcaggata
    11641 ttttttacga aaatggcgta ctgcttctgc agtttcttta cctgcactga tattgataat
    11701 cgaaatacca gctgctaact tttcatctat ttctgtatat ttcgacgtta ccgtactgat
    11761 aactggaata tcaactactc gacaaacttc ttttactgtc tctaaaggcg taggaccgtt
    11821 caaaacgacc cctaacgaac cttgcgcttc tgcgaacatg ctcatgtagg aagaacgagc
    11881 accttgtgtc aaaccaccac ctacaccgga gaaaactgga atgtcagctg cctcaataat
    11941 gcttttagta atcgctggat gaggtgtaaa aggatacaca gcaattactg cattcgcatt
    12001 tgtattacgt atgatcgcaa tatccgtagt aaagatgatc gaacggatca attttccaaa
    12061 tatcttgatc ccgcttgctt ctaaaatgat ttccggtact cttacgatat cttttctcaa
    12121 ttccgtcatg atttctggcg taaattgttc tgccatcagc cacttctcct ttgctatctc
    12181 cggtcatact gattcaattg atacgtctcg atgacctgga tgattttttg cgcataaccg
    12241 gggtctgtcg cataacctgc atcttgcaat gcttgtgcgg cttcctgata gttggtggcc
    12301 gtaatcactt tttcatacag cgcaggattc caatctactc cttggacgaa cagttgtgta
    12361 tggtcatcca tggactgttc ccatgaatca tacactttga agtctccttg gatagttatc
    12421 cattcttcgt taacaaattc ctttgtttcc aggcttactt tcttctgatc cccataagcc
    12481 ttgatcccga aaagattatt gtatttgcta gccaaggtac tttttcccca atttgattct
    12541 aatatagctt gtcctaaaat gatactagga agcactccat aaccatcttg tagttcttgg
    12601 gcatgcggaa caagccgttc aataaattgc tgatgggatt gcgcttcatt ttcttgctga
    12661 ttatcagtaa aaggagaaga cagattcttc aaggaaaaaa caaaggctaa gagtatcact
    12721 gctacaccgg caaccactgc tggccaacgg atattcttgc gcttttgctt tttccacttt
    12781 tttttcgcca tgatcaattc ctacttttca tttaattcat tcagatattc ttctagcgtc
    12841 aattggtgtt tggtgatata ctcagcgttt tctttaccga cataacgtaa gtgccaaggt
    12901 tcatatgtga tcttagttat atcttcgtgt ccttttggat aacgaacgat aaatccatac
    12961 tgcgcagcat tatctgcgat ccatttagct ccaggctgat ctccgtaact tgcatccaat
    13021 acagtagaag gataactgtt gtaccagttt tgatcgacga catcgactgc cagacctgtg
    13081 tggtgttcgc tgtatcctgg ctccgtaatc gtttctttag taatctttgt cgcctcctct
    13141 tctgatacac cttgacgact catcacatct tggacatttt gttcgaaaac ttgttgttgg
    13201 gatgatacag accggtaagc tgagaccatc actaatggaa atccagctgc ttcagcagcc
    13261 ttggcaaatg cttcatattc tggagcaatg cgtttgtcga tcaagtatcc gttacttaat
    13321 gatgccaatt gagtagactc atcaatttct ttttctaatt tgtgatctgg accaaccaaa
    13381 actagcttcc aatcgtctgt tttaacatct ggcaattgtt tggacgtacg atcttttgtt
    13441 tctttgtcct ttaccttgct gctggttgtt tgactgttct tttctttact agcagttgtt
    13501 ttggtgcttg ttcgttcgac tgcttgattg ccggcttgaa catgcgaatc atcatcgcta
    13561 aaaataacat agcccattgc tgcgatcata atcacaactg ctccaaatcc taaaaattta
    13621 ttcacttgaa aaatcctttc gttgttcttc acgttcccca ttcgcctcat gaacgtaagt
    13681 ttcttccaga ttttcattca cccaatcgag aagttcgatt ttttctcctt cgatctgttt
    13741 ttgaacatct gctggcagac gaaaattcaa aatatcatca tagccccatt catcatattg
    13801 aatacttact ttcaagtcaa ggtatctacc agcctgctga ttatttttca aagggatcag
    13861 ctcgactttt gctgctttgc tgctaagcca tttttgcgcg atgcgtgttt tgatttgctt
    13921 gtactctgca acggcaacgt ttcgtttttc gggaaaaata atcgtttgac gcaaaatctt
    13981 ttgcgttagc atccattcat aatcagtgaa aagatttttt actttctgca tcgcttcaga
    14041 tggcagattt ttctcgccat ctttccattt ttgccattgt tctggactga tcagcaagta
    14101 ttcatctaaa aaattttttt cagtttcaaa ctgttctaat aatacactca taataagatc
    14161 aatatatact tgatgcacgt ttatcccctc cttttaacta ttctacttaa gttttgatca
    14221 aaaagcacct cttaaatcgc tgctttcatt ttttttaatg tttcaagtcc tctcctcttt
    14281 attcttcgtg atttttgtta taataataga cagacaagaa actcaatcca actatgaagg
    14341 agcgttttca gatgaaaaaa tatcaatggg gaatcgttgg tcttggtacc atcgcacatg
    14401 agtttgccga aagcttcaat caagagacga gcgaactaac agcagttgct tccagaactt
    14461 cagaaaaagc ggaaaacttc gctcaccgtt acaatattcc aaaagcttac ggaagctatc
    14521 aagaaatgtt agacgatgct gaaatcgaca tcgtgtacat tgctgtacca aatagacagc
    14581 atatcgacca tatcttggca gcattggaag caggcaaaca cgttctatgt gaaaaagcca
    14641 tcacgatgaa taaaaaagaa ctggctgacg ctatgcgctt agcagaagaa aagaatttga
    14701 tcttagcaga agcgatgaca atcttcaaca tgccacttta tcagcagtta cgctcaataa
    14761 tggatacagg aaaactgggg gctttaaaaa tgatccaagc acctttcggc agttacaaag
    14821 aaccagaccc taaaaaccgg ttcttcaatc cagaattagc tggaggagct ctgttggata
    14881 tcggtacgta tgctgtatct ttcgctcgtt tctttttgag ctctcaacct gaagtagttg
    14941 cctctacaat ggttcctttt gaaacaggcg tggacgaaca atccgtcact atcttgcgta
    15001 acaaagaaaa tgaacttgct gctgtcagct tgacttttca ggcaaaaatg ccaaaagtcg
    15061 gtatcgttgc ctttgagaac ggttatgtga cgattgctga ttacccgcgt gctgatcgtg
    15121 cggagatctt atttacagat ggtacaaagg aattcatcga aagcgggaat acatctgaag
    15181 caatgaatta cgaaataaag aatatggtac acatgattga aggtgacttg cccaatcgct
    15241 ctctcttctt gacaaaagat gtcatcgaaa tcttggatca aatgcaagtt ctatggaaaa
    15301 atatgtaatt ttaaaaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    15361 acaaaaatag ctcctcctat ttttcgtttg gctggactta ttaccgcaga ggaaagcttt
    15421 tgaacaccgt ttaagaaaga ggttgtgagc ctaccgttca gctccgagtc acaaggagca
    15481 atttcaaaaa acagcttctc atgtttgttg aatattgtga cttgtgaccg aggagttgac
    15541 aggagaacac cgtttaaaga agaggttgtg aaatcagctt tttgacctga atattagctg
    15601 aattcacaac ctctattttt ggattttttg ttgggagaat tctgccttga tttcaataaa
    15661 acttttaaaa gactgcaaaa actggaacaa ttctgctcga tcctcgaatt cagggcgact
    15721 agtcggcaga ggcatttgcc gatacaatac ataaacttct tcaatacgtg ttaaaatttg
    15781 tctgccatca tttgactctg aaaatgtttc tgcagtataa atcaataaac catagatatg
    15841 ttttccataa taagctggtt gctggattct ctctagattt tccagcatgt ctttaatcac
    15901 attggcctgt gctcttctca tagagaagta agtttcgtag tatagcggct gtctgagcca
    15961 gtagttttct tgatgttctc tggcatcttc ttggctttcg cgaatgaatg tcagtaaatg
    16021 ttcgcatttg atctgcacat cttctttatt ttctgataaa agaaattctg ccatttgtct
    16081 caaaatattt cgaaattctt tttcgatctg tatttggttt tccttcagct gcttcgtacg
    16141 atctggcatg taaacatttg caagcaaagc aaatactaat ccaatcgcca ttaatcccgc
    16201 ttcattgccg atcagctgcc aagacatgtt ttcctccact aaataatgag tgaccaaaac
    16261 cgaatttact actatccctt cggttaattt gaagcgagag gcaagcggga tgaacaggaa
    16321 taaaaaaaga ctgaagccaa aaatagtata cccaacagca aaaaaactga acagtgctgt
    16381 tgccgtagct aatataaagg ctgcgattcg attcatgccc gtacgcaatg tagagcgctt
    16441 ggtatttcct acacttaata atgcaatgat ccctgcagct ggccaataca acagcgataa
    16501 gctacccgct gtgaccatcg aaaagaagac actgatcact gttttgactg ttcgcattcc
    16561 tatacgcatt tacttccccc ttttttattt taattatact tgaataatga aaaactagct
    16621 atcagacaaa agtttaggta tgatagtaaa aagaccaaaa aggagaatct ctatgcaatt
    16681 tggccgtttt cgactaggta tgcgtacgat caaaagtgcc ttagctgttt ttttatgtat
    16741 cttgtttttt catttgactc aacgaggaat cccgatgatt gctgcattat ctgctgtttt
    16801 ttctttgcgt caggacttga ctacaaccgt ttcttttgga aagtctagga tcatcggcaa
    16861 tacattaggt ggtacattag ctatcattta tttttttgtc aaaagctatt ttcacaatga
    16921 ttttttggta gaattgatct tgcttcctat tctagttatt attgtgatcg tcgtgtcaga
    16981 tggtattaac aataattccg gtatcatttc cgcaatagct acgttgttgc tgattgcgtt
    17041 gagcatccct caaggcgaat ccgctttgta tgctatccaa cgtgtactag atacttttat
    17101 agggacattc attgcaatcg gtatcaattt ctttcttcgt cctcctgaaa cagaaaagaa
    17161 ggaagaacta tcagaagatt tggtcgaact ccaaaagaaa gaacgattgc taagaacaaa
    17221 tttacaagag atagaagcta agatccaaaa acaaaagaaa aatagcgagg actaattttc
    17281 ttttgcaata aaataaaaaa atggagagaa gaactttaaa aagtcttcct ccatttttga
    17341 ttgaacggtg aagtgtacta ctttattctt ccttcatcga gttttgttct gcctcttttg
    17401 gcgcttcata catatttact tggctagtag tcccacgtgt cgacaaattc tctacgattt
    17461 ccttcaagtc aagtcctgtt gtcgctttta gtgtttcttg agtagaagaa agcaaatttg
    17521 tggcataatt tgtcactcgg ttcgctccac tggtttctcc tgaactgctt cctgtatcga
    17581 caacagagat tttttcgata tttcctagcg gctgtgctgc ttcgcgcatc aattgcggta
    17641 gcatatcgat aaccatactc aagacagctg cttcgccata ttccttgaag gcattggcga
    17701 tcttttgttt tgcttcggct tctgcttttc cttttgctag tgctgcttcg gcttctgctt
    17761 gtccagctaa acgtgtctta ttggcttccg cttctgccag tgcttccaca cggaatcgtt
    17821 ctgcttcggc ttcagtcact tctttgactt tttgagcttg tgcttcttgt tctttagcgt
    17881 accgatcggc atctgctttt ttcttcactt ccgaatcata ttgcttttca cgacgtgtga
    17941 tttctttttc ttccagttcg atttgttttt gacgttctac gattttaatc tgcatttcct
    18001 gttcgattac ttgttgttgt gctcgggcac tttctaaatt gtaggcttgg tctgcttttg
    18061 ctttagcaac atcttgttct tgtttgtagg ttgctaattt cagttctttt tcttttaatg
    18121 attctgcgat ctctgtttgt cgctgtaatt cagctgcttg tgattctttt tctgcttgag
    18181 cttttttgat acgcgtttct tttagagctt ccgcttctgc tatatctgca tctcttttga
    18241 cttgggcgat tcttggtttc cctaaagagt caagataacc gtttttgtct ctgacctctt
    18301 tgatagtgaa agaaacgatg atcaacccca tttttgcaag gtctacagaa gcgacttctt
    18361 gtacgctttg actgaactta tcgcgatttt gatagatttc ttctacagtc atagaaccaa
    18421 gaatcgagcg caagtgtcct tctaatactt ctcttgcttc attttccagt tcttctcttg
    18481 tcttgcctaa aaactgttct gctgctgtag caatctcttc tacagatgaa ccgattttga
    18541 tgatcgaagt accatcacac atcaccggta cgccttgttc cgtatacact tcaggggtgg
    18601 agacgtccaa tttactcgat aatagactga tccgattcga acgttgaaaa actggcaaga
    18661 cgaatgcgcc acctccacgc acaattttga ttttgttatt gctttcatca atgtgcacat
    18721 ttttgcttcc taaataactt ccactgatga tcaacgcttc atccggtttt gctgtctggt
    18781 acttcgctac aaagacgatc agcaacatca gtaaaatcaa agctgcgatt gcgatcgcga
    18841 taaaaacaac tggtatttcc atttaacaca cgctccttta agcttttatc atgctttcat
    18901 aaggcatgac ataacagatt ctttccttga tctcaatgat caatactttc gttccccgct
    18961 cagctgtctg ttcttggggg ctgtaaaaac aagctggacg actaatggaa ccggtaacac
    19021 ttttcaactg aatttctccc atacctcgta cagggatcgg tgtgatcaca acagctactt
    19081 gtccttccag agttttttca gaagacgaca tcgttgattc ggcatgtttc atcggcatca
    19141 atatatagaa attaagcaaa aataccaaca ctatcgatat gcctccacta gcaataaata
    19201 aaaataaaga tgattgatct gttaaacgct cacctagaaa accaaataag cttgtgaaag
    19261 ttaaccaagg gataataagc atcggatcaa gcgggccatc aaaatcgaat acatctccaa
    19321 aaataatcaa taggactgcc aatcctgcac aaatcagcaa tgtataccaa taaatcgtct
    19381 caagtgcgat tccccctatc atatacacgc ctccttccat ttctacgtct atcataccaa
    19441 tgtttgaaaa ttctgtatag cttcttttac cgactcagtg gtgtttttta taggaaggtt
    19501 gtgtttatcg ttgacaattt gttaaatttt tgttttcatg ttaaaatttt cacaataaat
    19561 tctttcgagg tagcctatgt ttcctttttt taagaaaaaa ccattaaagc tccagaacac
    19621 gatcatcctt gtcgtttgtt tctccacatt cgtttctttg attgtttctg ccatattgat
    19681 caggaatttt attctttcga tgaaccgatc tagtgtgtca gataaaatct ctagcattgc
    19741 taaaatcgtt gccaatgata aactcatcca aaaaaagctg cagactggtg acccgacaca
    19801 tgcgattcaa ggaataacaa atgaaatcat gattgcatcg aatttagatt ttatcgttgt
    19861 catggaccct gaaggcattc gatattctca cgcagaccca agttacatcg gtcttccttt
    19921 ctcaagtcat gaagatgtga aaaaagtgat tggaggacaa gaatttttct ctgaaaaaga
    19981 aggaatgtta gggttcggct tccgctattt cgtccctatt tattatgatc aagagattat
    20041 tggtgcagtt tgtgttggct taacaaacaa cacgattgac aatcaagtca aagatggaca
    20101 aaaaatgatt gttttttcat tgattattgg acttagcgta ggcatcatag gcgccgtttt
    20161 tcttgcccaa aaaatgaaaa aagtactgct tggacttgaa ccacaagaaa tagcagaaca
    20221 gttaagtgaa aaaacgatta tcgaaaatga agtagatgaa ggaatcttag ccatctcttc
    20281 tgataaaact atcctcttga tcaatacacc tgcaaaaaac attttctacc aaacagagca
    20341 gctattggat ttcgaagtag gaaaaaaatc agtgataatc tatatgacgt tttgtttaaa
    20401 gagacgattg agcatcaaac gaccgttcgg actcaatcca tgtatttaaa tgggatagaa
    20461 actatttcta gcgttacacc tctttttagc aatgaccgtt ttttaggagc agtcgcaact
    20521 ttacgaaatc gatcagaagt aatgaaatta atggaggagc ttggggggac gaaacaatat
    20581 ataaactcca tgagagctca aacccatgaa ttcatgaata aacttcatgt catttctggt
    20641 ttgattgatt tgaagaagta tgaagaagtc acccatttta tccagcagct aaatcaaaat
    20701 tataaaaaag agattggcca agtctcaagt ctgatcaaag tacctgcaat tgccggtttt
    20761 ttattaggca aaataaacga agccaaagaa caatctgttg aaatgctgtt agaaagcgat
    20821 tcttttatcc ctaaaatgga gatggatgac tccatccatt cacttttgca agtgttaggg
    20881 aacttacttg ataatgcaaa agaagccgtg ctacaaaaac atgaaagaaa cggacaggtc
    20941 cggattcatt taaactatga cacagaagct gaagtattca tcgtgaccgt ctgcgatgac
    21001 ggtgtcggga tatcagaaga aatctcagaa aaaatattta cgctaggttt ttcgacgaag
    21061 ggaatcaatc gtgggtttgg attgaatctc atccattcaa tcgtcaaacg ccacggtgga
    21121 tttttagaat tttccaaaca aacaaatggc ggtacaatcg tctatctaga attaccaaaa
    21181 aaggaagatc aaaatgatga caaatgtcct gataatcgaa gatgatccaa tggttgcaga
    21241 attgaatcgt acttttgtgg aaagcatccc taatatgaac gtaatcgaaa gtttcagaaa
    21301 tgtagaagaa ggaaaaaaat atttcagaca aaaaaacgca gatctgctgc tgcttgatgt
    21361 atacatgcct ggcgaaagtg gtatccattt tttatcttgg cttagacagc aaggaaatga
    21421 tatcccagtg attttgatca ctgctgcaga tgatatcgaa accataaaga atgtgatccg
    21481 ctacggtgcc ttagattatt tgatcaaacc gttcactttg caacgttttc agctggcgat
    21541 cgaaaagtat cttgctttct ctcagacaat ccaaacgact gaaaaagccg atcagaaatt
    21601 gatcgatcaa gtattcaacc acaccactcc cattcacgaa ataccatcat tcatcgattt
    21661 gcctaaaggt ctgtcaaaac tgactttaaa aaaaataagt gcatttattc taaaagaaga
    21721 tactattttt tcaacaaaag aactggctca aaaaagctca ctgtcacgta tatccgtcaa
    21781 gaaatatttg aattttttag tagaacaaga tatattgaac gaaagaatca aatatttgga
    21841 aatcggccgg ccgctaacct gttatgaatt gacaaagaat gggcggaaaa agctcacaga
    21901 ttactgttga gtcatcgaat tatcccatac aaaatagtcc aagtatacag aagtaacaac
    21961 tgtttacttg gactattttt aaacatttta ttgacgaata tccatgtttt cttatcttaa
    22021 ttgacgggct ttaggaagag atacctcatt tttcgttttt ccagtttcta ttatttcacg
    22081 aaagttctcg aaacttgtcg aaatcatatt tgataatgcc tcatctgtat ttgaacctac
    22141 atgcggggtt acaagaactc tagggtacat ttctactaat tgctggatag cagggtctgg
    22201 aatcgtctcc catggcttga aggatcggaa aaaaacctct gtttcatttg caaaaacatc
    22261 ggttgcaaaa ccagctaaat gattgctttt caaagctctt aaaatagctt cattatcttg
    22321 aagctctcca cgggctgtat taatcaagat gctacctttt ttcatttgag ctaaaaatcc
    22381 ttcattgatc atttgatcgt tttcacctgg gatatgaggt acatgaatac tgacaatatc
    22441 gcttttggct aataactctt caagcgagtg gaacgtcaat acttttttag cagcttctgt
    22501 ctgatgaata tcgtagccga tcactttggc tcctagtccc ttaaacaaaa cagcttccgt
    22561 caaccctatc ctgcctgttc ctatgatccc tactgtgcaa tttctgattt cacgactaaa
    22621 catttgctca tccactcgaa aatctttgaa agatgttcgg atcgtcgtat acgcagtatt
    22681 tctcaaaagc atcatggcaa aagtcaaaga taactctgct atcgcatttg gcgaatacga
    22741 tggtacacgg gctactgtca taccaaaatc agctgccgct tgtagatcga tatgattgac
    22801 tcccacagtc cgtgtaaaaa cataacgaat cccatattcc tgcatttttg caagattggt
    22861 ctcatctgct atacaatttc ccctcaacaa tacagcatca tggtcctttg ctgacacgat
    22921 attgtcatgt gtcaacagtt cttcataaag actgagatca taatggtaaa tattcaattt
    22981 ttcaaaaaaa tctatctcgt tcggacgaac accgtaacat gcgattctca tactttcttc
    23041 ctccatttct tatgaaaata aacctatgcc atgaatcatt tgtaatacaa cgatccatac
    23101 aggtgtcagt accacagcaa aaatagttga cagtaaggat gcattggagg ctaaaagtgc
    23161 ctccctatca aaactgatag cataggctgc tgccactgtt gcagctggtg tagccatcat
    23221 gataacgatt gttgccagtg cttcaaaatt gactggtaga atattcgttg ccgtcaaaac
    23281 tgctagaatc acgatattca atgcaggtac aaataccact ttgactagac tgtaatacca
    23341 tgaagttttt tcagatgcag cttttttgaa gcttacttca cctaatgttg cgccgattgc
    23401 tagccacgcc aatggtgaac ttaaaccagc taaataatcc atcggctgaa atagccacgg
    23461 tgcagtctgg tcaattcgca aaaacgctac ttggcttatc tctccttctg cacctgtaac
    23521 tgacacttgt ggcagatacc cttggaacag ccaaatgaaa aggccggcaa aagtagcgat
    23581 aatgattgga ttgacgaaca tcgctttgat atttttagct tccatcttca agccactcat
    23641 ttttatgtat ccataagaat acaaaaagat tcggtagcca atattgaaaa ttgaggaata
    23701 catcacgccg acaggaccat aaatcgcact gacgataggc gttccgaaaa aggtcgtaga
    23761 accaaagatc gtcaggacac gcaacgttgt ttgcttatcc cctttatagc ttaagaaaaa
    23821 aggttttgaa actagaataa gaataatata aataactaat ccccaaatca agacattgat
    23881 tccttgcatc agtaattctg tattgatatc ttgcatgaaa gctcgaaatg ccagtgctgg
    23941 caaagccact gttaagacga ctttcgatag aatttttcca acagattcag aaaaaatccc
    24001 cttttttcta caaacaaacc ctaataatat aataaaaact gttgaagtga ttgcactgac
    24061 aatcgacata tctgtcaaag tcgatcgaaa aatgtcaaat aagttcatct ttctcctcct
    24121 aattatgttc atttattcac aataaaaaaa gcttttattt tgctaatacg tattaacatt
    24181 tttaatttta tctttacatt gttacttatt gaacaatagg aaattaaaga aacttttgaa
    24241 acttatg
//
LOCUS       DAYYGO010000031        23869 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000031 DAYYGO010000000
VERSION     DAYYGO010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23869
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.031"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(116..478)
                     /locus_tag="ACHA5T_001045"
     CDS             complement(116..478)
                     /locus_tag="ACHA5T_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886636.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(552..932)
                     /locus_tag="ACHA5T_001046"
     CDS             complement(552..932)
                     /locus_tag="ACHA5T_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFM8886637.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1014..2189
                     /locus_tag="ACHA5T_001047"
     CDS             1014..2189
                     /locus_tag="ACHA5T_001047"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFM8886638.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2266..2850)
                     /locus_tag="ACHA5T_001048"
     CDS             complement(2266..2850)
                     /locus_tag="ACHA5T_001048"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8886639.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3051..4049
                     /locus_tag="ACHA5T_001049"
     CDS             3051..4049
                     /locus_tag="ACHA5T_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8886640.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4161..4670
                     /locus_tag="ACHA5T_001050"
     CDS             4161..4670
                     /locus_tag="ACHA5T_001050"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFM8886641.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4700..5359)
                     /locus_tag="ACHA5T_001051"
     CDS             complement(4700..5359)
                     /locus_tag="ACHA5T_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFM8886642.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5361..6125)
                     /locus_tag="ACHA5T_001052"
     CDS             complement(5361..6125)
                     /locus_tag="ACHA5T_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFM8886643.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6133..6810)
                     /locus_tag="ACHA5T_001053"
     CDS             complement(6133..6810)
                     /locus_tag="ACHA5T_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFM8886644.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(6847..8232)
                     /locus_tag="ACHA5T_001054"
     CDS             complement(6847..8232)
                     /locus_tag="ACHA5T_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFM8886645.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8442..8526)
                     /gene="ffs"
                     /locus_tag="ACHA5T_001055"
     ncRNA           complement(8442..8526)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHA5T_001055"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8549..9064)
                     /gene="tadA"
                     /locus_tag="ACHA5T_001056"
     CDS             complement(8549..9064)
                     /gene="tadA"
                     /locus_tag="ACHA5T_001056"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFM8886646.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9171..10028
                     /locus_tag="ACHA5T_001057"
     CDS             9171..10028
                     /locus_tag="ACHA5T_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8886647.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10109..10444)
                     /locus_tag="ACHA5T_001058"
     CDS             complement(10109..10444)
                     /locus_tag="ACHA5T_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886648.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10511..11881)
                     /locus_tag="ACHA5T_001059"
     CDS             complement(10511..11881)
                     /locus_tag="ACHA5T_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFM8886649.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(11959..12495)
                     /locus_tag="ACHA5T_001060"
     CDS             complement(11959..12495)
                     /locus_tag="ACHA5T_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8886650.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            12804..14285
                     /locus_tag="ACHA5T_001061"
     CDS             12804..14285
                     /locus_tag="ACHA5T_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8886651.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLIKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            complement(14388..15095)
                     /locus_tag="ACHA5T_001062"
     CDS             complement(14388..15095)
                     /locus_tag="ACHA5T_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFM8886652.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(15174..15512)
                     /locus_tag="ACHA5T_001063"
     CDS             complement(15174..15512)
                     /locus_tag="ACHA5T_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFM8886653.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(15623..16585)
                     /locus_tag="ACHA5T_001064"
     CDS             complement(15623..16585)
                     /locus_tag="ACHA5T_001064"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFM8886654.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(16600..17931)
                     /locus_tag="ACHA5T_001065"
     CDS             complement(16600..17931)
                     /locus_tag="ACHA5T_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFM8886655.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(17939..18619)
                     /locus_tag="ACHA5T_001066"
     CDS             complement(17939..18619)
                     /locus_tag="ACHA5T_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFM8886656.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(18838..19317)
                     /locus_tag="ACHA5T_001067"
     CDS             complement(18838..19317)
                     /locus_tag="ACHA5T_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFM8886657.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(19444..21123)
                     /locus_tag="ACHA5T_001068"
     CDS             complement(19444..21123)
                     /locus_tag="ACHA5T_001068"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFM8886658.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            21671..23173
                     /locus_tag="ACHA5T_001069"
     CDS             21671..23173
                     /locus_tag="ACHA5T_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8886659.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            23186..23848
                     /locus_tag="ACHA5T_001070"
     CDS             23186..23848
                     /locus_tag="ACHA5T_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8886660.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
BASE COUNT         7154 a         4911 c         4057 g         7747 t
ORIGIN      
        1 taaaatgcac taaaaaacta tctttagcag aacatttcat tgtatttttt ggaccaaaca
       61 ataaaaaaag aagtttgaaa aaaattcaaa cttctttgtt cgcttaatct gtattttaga
      121 agaaaggcac tccctctact tcgtaagttc caagaacaga gcgcatttga cctggttctt
      181 cccaaatata tccttcgcct aattcttctc tttgattctt ttgttcagtt tcaaagacaa
      241 acaacggaac attttcctct ttaatatgtg tatttgtcaa ttccatcaaa ctaatcttgc
      301 taacatcgat atgaactatt tcttttaaga aattaagtat attcgctaaa ccagtctttc
      361 cttcagaaag ttccgctaac gcaaattcca acttgtcatc aattgaatgg accaaaaatt
      421 ttttagtacc atcctccaaa tgcaacataa ttgtaccagc aactcttttg cttgccaaaa
      481 tttccacctc gatcaacttg ttctatggct tacataaaaa ttctagcaca ttttcagaaa
      541 aaatacactt tttatttctt attttctttc cgttctagtt cagataatgc ttcttctatc
      601 caaataggta actgctccgc aatagtggta aatccgatct tacgattttt attcgtgaaa
      661 tacctttttc tgcgatatgg aacagctgga tttgtttttt ctaacgttct taaagataaa
      721 ctgatttttt tggaatattc atcaatgtcg atgatctgaa ctgttactgg ctgtccgact
      781 tttaacaatg aatggatatt tttcgtataa ccggattgta cttctgaaac atgaataagt
      841 ccttgcgtct cgttatcaag agaaacgaat gcgccataag gctgtatccc tgtcactttt
      901 ccttcaatta tttgtccaat tttataagtc atctacttcc ctctcttcgc atttatcatt
      961 atactgtaaa tagctcaaaa aaagaagcga cgaaaatgac tggaggtctt aagatgatcg
     1021 aatttgataa acagatttca agaaaagata cagaaagtgt aaaatgggac gcaattgctg
     1081 agacatatca aatggatgac ttacttcctt tgtgggtagc agatatggac ttcttgtctc
     1141 ccggtggggt agctaaagcg tttagtgaat acatcaaaca tggaatcttt ggttatgcca
     1201 ctctgtctga taaactatat caggctatca ttcattggga acgccaacac catgctgtgg
     1261 agttgacgaa agaaaatatc gtttttacaa gtggtgtgtt aaccagttta gctgcagcag
     1321 tacaaacttt tacaaaacca ggcgattcta ttttgattca tgacccagtt tatcctccat
     1381 tttcctctat tatagaaaca aatcaacgtc acatcgttcg aagctctttg ctagaaaaaa
     1441 ataatcattt catcatggac ttgtctgaca tggagaaaaa aatcgaagaa aacaatgtaa
     1501 aagcaatgat tttatgcaat cctcacaatc ctggagggcg tgtttggagc aaagaagaat
     1561 tgaaacagtt aagcgaattg tgtttaaaac atcaagtgat tctgtttagt gacgagatcc
     1621 atcaagattt aactttattc gatcatacat ttacttccat gctaacgatt gatcctaatt
     1681 tagatgatct actagttgct tttacttccg caaccaagac tttcaatcta gctgcaatca
     1741 aaaattcaat ggtatttatc aaaaatccag agatgaaagc tgattttgaa aaacatttag
     1801 tgatgaacca acagcacgaa atcaacactt ttggactgat tggaacccaa gcggcttatg
     1861 agacgggtgg agaatggtta agccaactga ttccttatct agaaaaaaat gttcaaacag
     1921 ttaatcagtt ttttgaacag tatcttccga aagtgagaat aatgaaccct gaaggaactt
     1981 acttaatgtg gttagatttt accgcctata tttcagacga ccaagaattg gagaacactc
     2041 ttgttcggaa aggaaaagtc gtattaaatc ctggcatcac gtttggtcct tccggtcatg
     2101 ggcatatgcg cttgaatatt gcttgtccag aagaaacttt actggaagga cttaatcgaa
     2161 tcaagtatgc tttagagaca atcgattaaa tcatttattc aaaataaaag gacgagacct
     2221 tactaggttt cgtccttttt cataattact ccgtaccata ccaaactatt ctgaaatttc
     2281 aattgtttca atcactacat catgtacagg tcggtcttgt ggaccacgtt gtacatttgc
     2341 gatttcatct actgtgtcca tcccttctag tacatgtcca aagactgtat gtcggaaatc
     2401 tagccaagga gttccgccac ctttatacgc ttcaatgatt tctgatggga atccagcacc
     2461 ttctagttgt ccaagcatat tagctggcac atttgtgtta tttacaataa agaattggct
     2521 tccgtttgtg tttggacctg aatttgccat agataaagct ccgcgaaggt tgaacagttc
     2581 acgactgaat tcatcttcaa aactttcgcc ataaatactt tcgccaccca ttcctgttcc
     2641 agttggatcg ccgccttgga tcatgaaatc aggaataacg cggtgaaaaa tcacgccatc
     2701 ataataacct tttttggaaa gttcaatgaa attttgaacc gtcttcggtg ctagttcagg
     2761 gaataattga acagtaatgt caccacggtt tgttttgata acagcttttg gtcctttttc
     2821 actttttaaa tctagttgtg ggaatgccat attgtttcct cctaatattt gcatacttca
     2881 taataataat aacataatac ctaacctctc gtaaaaatac tagatttgtt ttcttttatt
     2941 tttcatcact agatacattc tataacttac cgtttcgatt tcgaattcaa acatttttga
     3001 gaagcattct caattaaatt ctgatatagt gtgaaaaggg aggaattttt atggaaatct
     3061 atgacattac aatcatcggt gcaggtcctg tagggatgtt tgccgctttt tacgcaggaa
     3121 tgcgccaagc taaaacaaaa atcattgaca gtctacctca acttggtggt caattagcaa
     3181 ctttgtatcc cgaaaaatat atttacgata ttcctgggta tccagcaata aaagcaagtg
     3241 aattaatcga tcaattagaa aaacaattaa ctacttttaa ccatacattc catttaaaag
     3301 aagaagttct ctcgcttact cgtgaagacg aagttatcga gattactaca aataaaggca
     3361 tccattattc caaagcagtc attttagctt taggaaatgg ttcattccag ccaagaaaat
     3421 tgaatttaga taatgctgaa tcatttgaaa accacggatt ggattatttc gtgaatgatt
     3481 tgatgaagta tgctggaaaa aaagtcgcta ttgctggagg tggtgattct gctatcgatt
     3541 ggacgttgat gttagagcct attgcaagcg aagtttactt gattcatcgt cgcccggaat
     3601 ttcgtgcgca cgaacatagt gtatcacgct tgaaatcttc atctgtcaac ttgcttactc
     3661 cctatctcat cgatggatta tcaggcaata atggggaact gacagatatc cggttaaaaa
     3721 aagtgaaaag tgacgagacg attgatttga tgattgattc tctgatcgtc aattatggat
     3781 tctcttctaa tttagaacat ctttcttctt ggggacttga tagtaccaga aatgccatta
     3841 ctgtaaaatc agatatgtct acttctattc caggcgttta tgcagctggt gacatctgta
     3901 cctatgaagg aaaagtgaaa ctgattgcta ctgggctcgg tgaagcacct actgcagtga
     3961 acaacgcttt acactatatt aatccaaagg aacgaactca gcctggtcac agcacaagct
     4021 tatacgataa aaatcttcgt cctagttgat tttttatatc tctttgcatt tcaagttaaa
     4081 cccgaaagca gtttcttgca tttttttcat gttccgttaa catgaaatag agaaagaaaa
     4141 aataaagggg gagcaataat atggtcgtca aaactgagac tttagaagaa aaggcaagag
     4201 aactattgaa ggaacgtggc gtaactgtag aagatatcgc acaacttgtc atgtttttac
     4261 aaaaagacta tatttctgat ttgactttgg cggattgtac tgaaagtgtc aacagcgtgc
     4321 taacaaagcg tgaagtacat aacgccatta tcactggaat ccaattagat atattagcag
     4381 aaaagaaaaa attgatgagt ccgttgcaac atattatcga agatgacgaa ggcctttacg
     4441 gtatcgatga gatcctagcc ttgtcaatcg tgaatgttta cggtacgatc ggctttacga
     4501 attatggtta tatcgacaag gtgaaacctg gtattttaaa agaactaaac agccacgatg
     4561 gcgttcatgt ccataccttc ttagatgata tcgtcggagc gatcgccgct gctgctgcca
     4621 gtcgattagc acacaaagaa ccagaaaaaa gaagtcgcct tactcaataa ttcaacaaaa
     4681 aaagaaagaa aacgtctatt tagacgagtt ctttcttttt tgtttgtttc aagctatttt
     4741 ttcctaagaa aagaaaaaca gcaaaaaata attctaataa tacaaaaaat aagataaagc
     4801 tatgcatgaa aaattcttct ggaagtacat tgatgattgg atcagtaacg ggatcgaata
     4861 accagtcatc gttgttgaaa aacgtttcat gaaaaaggat aaaaaagcga tcaaatccta
     4921 tcatcatgaa aaaaccgaaa acaactggca gtaacatgcc gatttgaaac ggacgaatca
     4981 accgccaaag ccgatcattc ttttgcaaat atttgataaa caaaatactc gggatcaaag
     5041 tgatgaaaaa cacagcataa tccactaaaa acaaaatttt cacttcataa aagtgatggg
     5101 ctccgctagc agaaactggg aaatctggca agctgaggat ggttttgaag ggattattga
     5161 gataattcat taggtgatca aaatttttca gtaacgtctc ttgatctaaa gaagtataat
     5221 ccaaaatttg caaatgtccg atatcaaata cgtatagtgg tcgaaagttg atcgtcacac
     5281 caactgccag actgatcagt gtcagaaata ggctgaacaa tccagcatat tccatccact
     5341 gccatctcga atttttcata ttagaagctc cattcatcca gtgattgttt cacataggta
     5401 gctgggatag gcaacccagg tacatcttct tcttttgtaa aacctgataa aacgagcaaa
     5461 gtatctatat tattgcgtat cccagcttgg atatctgttt catagttatc tccaaccatg
     5521 attacttctt ctttttgcag ccctaatact tcaacggctt tatccataat gatggcttct
     5581 ggttttccga tgtagatcgg cggcgtttgg gttgctgtct ctacaaaaga gatgactgac
     5641 cctgctccag gaagaaggcc gcgttcagta ggtatattct tatctgggtt cgttccaatg
     5701 aaagtagcac ctttttgaat agcaagtgtt gctttaacta ctttttcata ggtcagataa
     5761 ttatctaagc ctaccactac atagtcagga gcctcttctt cccatgtaaa tccggcagct
     5821 aaaatcaagt caatcaatcc tgcttctccg ataacataga cttttttccc ttttccatct
     5881 gctttcataa aatcgattgt tgccaaagta gctgtgtaga ctgtctctgg agctacatga
     5941 atgtcaaact catctgctag acgattagct actgtttctg gtgacttagt cgtgttattg
     6001 gtaacaaaca agaaaggtaa ttttcttttt tgcaattctt ccacaaatcg tttaccggcc
     6061 ggtatgggtt gtgtcccacg ataaatcgtt ccgtctaaat caattaaata tcctttatag
     6121 tccattcatc cactactctt ccctttttct gatcgtaaat gctgtttttg ttttttctac
     6181 ttgtttaggt tgttcttgtt tttttacttt ttcagaggtt tttgctgact ctgtttttcg
     6241 atttttaata accggttttt tgcgattaga tgcgactggt ccttttttct catcgatata
     6301 cgcacgatta tggttttttt tcttttttcg gcgctgagtt gtcttttctt ttttaccgcc
     6361 gattctctcg atgacaaaat acgcacaacc aaaattgcaa tattcgtaaa gatagtcttc
     6421 caaggaatcg attcgctgat ctggatgcgc ccgtcgatta tccgcttgga aaaatccttt
     6481 taggcgtagt tgttcataac cccaatctcc tacgatataa tcatatcggg ctagtacatc
     6541 actgtaacgt tctccaagct tttctgcatt aaatccttca cgataatcgg acacaagccg
     6601 ataatgtctt tcacctatca aaaaattact gtcatctaac agtgtcacta gctcgccttt
     6661 gactttttct gctttaggct cctcaacgat ttcttccaaa gcagctgtca gttcttcggt
     6721 gactgttgtt tcctgttcag ctgatgattt tttcttagca atttctgttt tatgctcaac
     6781 gttctgctgt tcattttgtt ttgcagacat gcgttcacca cctatctttt ttattatacc
     6841 ttgtttttat ttgattgggt agtgagcttt caagtaatct cctaccacac ttcggataaa
     6901 ttcagggaac aaaaattcat tttttccagc aatctcaatc gttggaaaaa atggaacaaa
     6961 catcaaatga tctacggtag taaaagtata atttttattc agatcgattg gcttgttctt
     7021 ccaaaagact tgatggttaa tagaatcata tgtgattcca ttatagacca tctgcccaaa
     7081 aatcttaccg cgaaatccca ttcccttcat aggatagtta cgtaaaaagt tacgattttt
     7141 ttctatttct aaaactaagc ggatcagatc atttccaccc aatgtgacat ttaacaagtg
     7201 catcggatgc ggcaacgcag tgtgaagctg atcctgattg acccgccctt ttggaatttc
     7261 agttaaaaac agaccactgt ttaatattgc tgcttccgtc tttcctcgtt ctttcaccgc
     7321 ctcaaaggct acttggataa acgaatgctc attgaagata tctagactca gagaaaatgg
     7381 caaatctgct acttcttttt cttttaataa ttcatgccca cgttctagat aactatttat
     7441 ttcttgttca tcttctgtaa aagctgtcat tgtttcggta gggatggctt tggctgaatg
     7501 cttaaggatt tttttctctt catcgatggt taaatggact tcaccgacat actgcccgta
     7561 ctttccagcg gctgctattt ggacatcatt gattacgtga ccttctctga ataaatgatg
     7621 ggtgtgcgat cctaaaataa catcaattgc tggtatttct tttgcaattt ggaaatcatc
     7681 ctgaatcccg agatgactca tcaaaactaa aacatccact tgtgaacgta aatcttcgac
     7741 taattctggc aaaatatcat aaggattccg tatgtcccag ccattcggat tataagtaag
     7801 cggaaatgga gcagtaaatg caattagtcc gattttcgtc tggtgtttcg ttgtaatgat
     7861 tttatatggt ttcgtccatg ccggtggctg gagcgtattt ttatcgaata agttatccag
     7921 gatgatatca aactcagcat gatcgtataa atgattcaac tcttctttgg aatttcctac
     7981 gccttcattg tttcctatcg tcgctgcatc ataaccaact tcattcatca gcaagatatt
     8041 ttcctgtcca ttcgttgctt ctgacaatgg atgccaacga tccacaaaat cacctagatc
     8101 tactgcaatc gttgtcgttt tttctttctt ttcgttttct cgtttacgtt gatccaaaaa
     8161 ccttctgatc cttggccaat tttctaaatg agaatgcaga tcattcgtat gtaaaatcac
     8221 tattttttcc atgtaccctc tcgcctctct ttatctttat cataccatag gcagcttttt
     8281 cttccattga aacaagctct atttcagagt ttttcctgcc ttgaagcaaa ttgtataagt
     8341 acaatattcg ataaatcagt ctggctaaaa aatcaggaac attcattttt acagtactat
     8401 aaaaataaaa aaaacagacc cgaaagtctg ttgtcgtttc attataatca gacacatggc
     8461 ggaacctgct tagtgctgct tccttccaga cctgacacgc ttcacaagcc caccattgtc
     8521 ctagcctttc ggcaaagaat agtatacctt atttttcatt cgttgaccag tcttttttta
     8581 atttcttttt tcgctggcgg agctttcgga aaaagtcagt caaaatcatc ccacattcgt
     8641 cttctaaaat gccaccttct acatatgctt ggtggttaaa ccgctcgtcc tccaaaagat
     8701 tcatcaaggt accggctgtt ccgccttttg gatcatacgc gccgaaataa acttctgcta
     8761 cacgagacaa aatcatcgcg ccgctgcaca taggacaagg ctctaaggta acaaaaagtg
     8821 cagcatcttc caagcgccag cttcccactt tttcacaggc ttccttgatt gcaagcattt
     8881 ctgcatgtgt agtagcatct tgcgtggttt ctctaaggtt atatcctctt ccaatcactt
     8941 ttccatccaa tacaaccact gctccaatcg gaacttcatg aagcatttct gctttctttg
     9001 cttctgccaa tgcaaggcgc atccattttt ctttttctac tgcatcaaga tttgtattta
     9061 ccaattaccc ttccctgcct ttgttttttc ttgttttact ttagcatatg ataaaaaggc
     9121 tgtaactaac ttttttttct atggtaaaat aaatacaaga ggtgaacaag atgaaaatgg
     9181 ataaattact tgctctttac ccacaagcac agaaaaaaaa ctctccagca tcagaagcgg
     9241 atatcctttc ttttgcaatt gatgatgctt ttatctggat caaacaagat tcattatctc
     9301 aacaagaaac cagccttttg aaagcgttat tccctgtaat caacgatcaa aaaaaacacc
     9361 catggtatca ctatcttttt aaagatgctt cttgtgttca agaaaaaagc tttcgtttga
     9421 tccagcttca cgtcgaatca aaaggagaat tcttaaaaaa agaatggcaa gatactattt
     9481 tggaattatt tcgctattct gctgatttct ttttttatac agacacagat gcgctcttag
     9541 tcgaacaaat aaaccgtgat tatcttgatg ctactgaaat caacggtatt tttctatctt
     9601 tagatgctga ttttgatacg acaacgtctg tattcgttgg ttctttccat tcacctggca
     9661 ccgacctcgt ccctttgttt gctgaagaaa gaaaaatttt tttaacagag caaaacaatc
     9721 tttatactca cagccgtact ttttctatgc aagatgtggc tttacattac tatacaaaag
     9781 atactgtaaa agatagtaag ttgatcaaaa gttacataga actgatcaaa caaaatgaaa
     9841 tggaagtgat cattcaagaa ttatggaatg aattaggcaa tgtcagctct gctgcaaaaa
     9901 cactgtttct tcatcgtaat actctaaaat atcgaatcga aaaatttcaa gaacagtctg
     9961 gtttcaatct aaaaaaagcc aacgatttac tgttttgcta tcttttattg cttcatgaac
    10021 ataattaaag atagattaaa ggggttgtga caataatttt gtcacaaccc cttatttcga
    10081 atgaatagtg ttcaaaagct agtttctctt acatcatttt ttcaacaaaa tgcattagcc
    10141 ggtcttttac atcttcggtt gaatctttga ttgaatcacc ataaacagag attttctttt
    10201 ttcctgattt gatttttgct aacattccca tcaaagaatc caagtcacta tctgaaagac
    10261 catctacgat ttctagtaat ttttcatttc catcaaattt gtcatagacg aattttttta
    10321 ctttcgtccg agtagtcata tgatggatct tttccattac ttttcccgat acaacgactg
    10381 ctgtcgctac actggcagcg gctgctacgc ttaatccgat tgttactttt gttgatgttt
    10441 tcataagtat cagctccttt ttatctatct ccatcatagc atagacaaaa aaataaacga
    10501 actaatccac tcagtgaaat ctcaatcctt tagggaaaaa gttctttact gttcctttta
    10561 cttgtttccc aaatccgaca ggtatcttat ctaatactag caaaacccat ccttgattcc
    10621 catcacgttg aaatgtttct cctgctgtgt aagattgcca ttccttttga gtgatcggca
    10681 agcaaggtat attctctatc ttttttgtag caagcgctaa tgcatagcta ggttcaaatc
    10741 gattcttttt gaaatctcct aaatgcaaac ctgtacgtac gacttttaaa ccatcgagag
    10801 aaggggctaa ctcaggaact tcccaaagat ggtcgttgaa cactaaaagt cttccagtag
    10861 cttcataatg aaaatcattt gaaaactccg tccagagttt ttcctgctct tttgtcattt
    10921 gcactttact tttttttcgt gtcttctttt ctttatgcat ctgattttgt ccatgaaaag
    10981 tcagctttgc aacaaaatgt ccttcaccct gatctttatg aggccatata cgtattgttt
    11041 tttccaaacc agctacgctt ccccattcag aacgtcctga actaacggat tgagtcaaag
    11101 gaatctcctc aatcgttact ggataatttt ctacaagcca gctaatgatc tcttcatttt
    11161 cttctggtgc aaacgtacaa gtcgaataaa tcagttgtcc tttatttttt agcattttta
    11221 ttgctgaaga caaaatctct tgctgacgtt tttgacagta tagcggagat tcttctgtcc
    11281 actcttttat tgcatttgga tcttttcgga acattccttc tcctgaacaa ggagcatcaa
    11341 ccacgatccg atcaaaaaag cccgaaaaat gagggacaag ttctgcgggt gcatgatttg
    11401 tcactattgc gttcgaaact ccccatcgtt ctatattttc tgataaaatc ttcgcacgtt
    11461 tcggaaagat ctcattcgtc actaataacc ctttcccctt catttgagca gctaattggg
    11521 ttgatttacc accgggagcc gcacacaaat ccaaaacttt ttcaccgggt tttgctgcag
    11581 cagctgttcc aacgatcatt gcacttggtt cttggctata ttcataccca gcttgatgga
    11641 ggaaagactt tccattgaca gtgccaagaa atccctcatt gctgtaggga gcgggttgta
    11701 attcttcgct atggtaagtt tggaccatgt ccaatcctgc cggttttagt ggattccacc
    11761 gaaatccttt tttcacgctt ccttgttcaa gcgcagaaaa aaaatcggat gcttcttcgc
    11821 ctaaaagaag acgatatttt ttgataaatt gctgtggtaa cgttgtcgct tcctccttca
    11881 ttccaatgtt tcgttagaca ttttacaatc ttttttacga aaaataaagg tggctgatct
    11941 actttttcat gttgctgcct aaggtgactg ttcctttaag atattttcca agtattcatc
    12001 cgtcacctcg tttttatccc aaacagcgtt catcatttca agaaaatctt gagaagaatc
    12061 atctgagcgc aaacttccca atgcattaag ccataccaat tcaagcgact tttcttcttt
    12121 gatcttcgtg tgacctttga catatgagaa aaatggccgc ttaaatagcg aatccatgtc
    12181 taaatcagat tttgcaatga tttcacgaat actttcatgg atagaaaaaa aatctttcaa
    12241 taagtagaca atgcttaaaa catcatcgga actgcattca gctaagtagt tttcaaaata
    12301 aacagtgatt tcttctaata tcttctcttt taacccgctg attgatttga aatgtgtata
    12361 aatcgggtat gtagaacaac cgcagaactc tgctaactct cttgcagtaa ttgacttcat
    12421 tccatattct tccacgtata ccatactttt gaacagcaaa tattctttac tataatttgt
    12481 tactctcccc atcattctta cctccgtctc ttttcatcta cccataaata aataacataa
    12541 ataattctaa aaatattatt gataattatc aaaacatatt tagttatatc cattaaaaat
    12601 caatttttcc gccttaaaaa caagaatatc ttgacaaaat accctaggaa ataaacaata
    12661 gaataaacaa ggtaacaaaa aaattaaata acgttatata aaaaagaagg cgacactctg
    12721 tgagaataac acagactgaa atgactttcc actcacagtt tttgtctaac tatatacaaa
    12781 aacagaataa aaaggaggta tttatgctaa ttgaagcaag aaaaaaagag ttacttgttc
    12841 tacaaatggt cattcaaaaa aataattgtt ctttacaaga attatccaat gacttacata
    12901 ttccaaaacg gacaatcaaa gaactgatcc gaaaaataaa tgtatcactc aagcaatttt
    12961 tattgattga tgaatttatt tattctaatc ataaaggaga gatccaaacc aaagaaaccg
    13021 atcaaactaa aaagttagct gttttttcta agttaaaatt atgctaccta aaagaatcca
    13081 atcgttttaa ctatttgatg ttactaatca attatccaca aactgctgta ccaaaaaaat
    13141 atttacttga acaactttat atatcgccct cctatctcga aaaactgaca aaacagctca
    13201 atcattcctt aataaaattc catttacgga tcctatctgt aaaaaattgc tgtactcttg
    13261 aaggaaatga gttatctatc cgtctctatc tttacttttt tctttcagat acatttggag
    13321 gtaacgattg gccattttcc tcacttgaat taaatgcact aaaaatgaac agtacagatg
    13381 agcaaataga aaaaatttca aaacaagaaa aaaatatcta cttgctgtta actttattca
    13441 tattacgaaa caaacaacag gcatattttc caaaaacttc ttctgaaatc actgaattga
    13501 tggacttatt gcagcataca catgattatt cagaatactt tacttctttt tcagtgagtc
    13561 gcccttgtga agagacagaa acagagagag cctactttaa ttattttttc tattgttttt
    13621 ttccaaatca aatctctttt cgacaaaaac aggagattgg ttttttattt tccacaagca
    13681 aaaaccttat ttgtcgacaa gtcggtcaat tgatgactta cttaactcaa cagtttcctc
    13741 tcaaccattt agaaaataaa ataccaattt atttttattt tcttgtgttg gcagttgttt
    13801 ttttcgaact gttgggtgaa tccagcagta gtttttctac actttgcttt ccagaagcca
    13861 cgtccaatca ttctagttct tttatacagg aaaagaaatt aagagaaaaa atcgatacaa
    13921 ttttttcaaa aaatggggaa agaaaccaaa tcagtcaata ccttagtcaa ttgatccaac
    13981 acttactaca aacagaaacg aaagagcagt tactcatcca tttgcagata accaaacagt
    14041 ttacaggaaa ttatcatctt caaagccatt tgcagcagtt atttcgcaaa gatgctgtta
    14101 aaattaccgc atattcagaa catgctgatt tgatcattac agatcagcat gagaaaattt
    14161 cagatgaaac ggagactttc tatctggata caacgcacaa tacagtgtta tttggcaagt
    14221 taataaaagc ggttcatgaa ctgtatatga aaaaaggtta tgagaacaaa acaaagagga
    14281 tgtgacaaaa ttcttgtcac atcctcttat tccaaataaa tggtattcaa tcagctaact
    14341 ctcggtatta gctcagatca aagcgctgat ttcacaactt ctttatttta atctcttcgg
    14401 ttgccgccga acaataagat caagcgcaac agctgcagga atgtcgaaag cgctgctgca
    14461 acataagtca gtgctgctgc aaataatacg tgtcttgcca tcggtacttc ttcttccgtc
    14521 agtagtccgc cttcactcaa aatttggatt gctcgccgag acgcgttaaa ttcgactggt
    14581 aatgtcacca attggaaaag taatgctaaa gaaaaagcga aaatcccgat attgattaac
    14641 gtagagttcc agctaagcaa tacacctacc ataatcaagg ggaacgaaat catcgaacca
    14701 aagttagctg ctggaacaag tgctgcacga atcttcaatg ggacataacc cgaagcatct
    14761 tgtaccgcat gtccgcattc atgagctgct acacctatcg cagcaactga agtagactgt
    14821 gcagttgctt gagacaaact caacatctta ttacctgaat tgtagttatc tgtgagatct
    14881 ccggcaattt gttgaacacc gacattattt atctgttgac tttgtaatat atattgtgct
    14941 gcttgtgtac cagtcacatt gtttttgctt ctcacttgat cgtattttcg gaaagtacta
    15001 ttgacgtacg ctgacgcaat tccggaaata actaatccaa gaatgaccaa aagaaatgtc
    15061 ggatctaaaa aaccatacat tccataacca tacattttcg tttcactcct tttcgtttac
    15121 tttattttat cattagtagt aggaaaagaa tgtgaaaaga ccttgaatat tctttaattc
    15181 tttttcacaa attccgattt cagtttcatc ggtccaaatc cttctacctt gcaatcgata
    15241 ttatgatcgc cttctactaa acggatgttc ttgacttttg ttccttgttt aatggcacct
    15301 gacgcacctt ttactttcaa gtctttaatc actgtcacgc tatcgccctc taacagacgg
    15361 tttccatttg catctaatac cactaatccg tcttctatga cagtttcttg ttcttctttg
    15421 ttccattcgt tcccgcattc tgggcaaatc aacaagcctc gatcttcata tgtgtactct
    15481 gagccacatt ctgggcaatt tggtaatgtc atcttctttc tcctttaaaa tctttagtga
    15541 gcactattgt aacggaaaaa gaagatcttg aaaagataca tttgtgattt ttactacaaa
    15601 aggcacgttt tcgaacgaag tatcattctg tttttctgct cttatacgat tggcatatat
    15661 cttggatctc tttatagatt tcctctactt ctgctaaatc ttttgcattc gcaccacttg
    15721 ctaatggatg gcctcctcca tgatggcgtt tagccaattc attgatgatc ggccccttag
    15781 aacgtaaacg tacgcggtaa taccctgaag gctgctgaac aaaaatcccc caagccagca
    15841 cttcgtcaat cactccagga agtgaaacga tcgaagctgt ctctgaatca tcgattccat
    15901 atttatcaag aatttcttgc ggaaggatta ttcttcctgc accattttca tccacctgga
    15961 tattttgata aagataaccg gaaagtttag cgactttcat cggcatttgt tcaatctctc
    16021 tgttcaaagc acttgcatca aatggataat ttctcaatat cgaagcaatc tctaatgtac
    16081 gggacgtcgt ggagggatac aaaaagcgcc ctgtatctcc gacgattcct gcataaagta
    16141 accgtgcagc cttaacgttc atctttaaag attcaggaaa catcaaagca aattctgcaa
    16201 tcatctcact acagctactt gcttttgtat ttacccacac aagatctcca taagggtcat
    16261 cattgggatg atggtcgatt ttgatcagtt tttctgccaa ctgatatctt tgatcactga
    16321 ttctgggtga attagctgta tcagtcacga tgactagcgc tccttggtag aagacatctt
    16381 caacttcttg catttctgct aaaaagcgaa gcccttcaat cgtttcacct acttgacgta
    16441 tttctttttc tgggaaactt tcccgtaata gttcggctaa tcctacttgt gatcctaatg
    16501 catcaggatc agggcgcaaa tgcctgtgga taatgatttt atcgtatttt tttattgtat
    16561 ctactatttc ttgctgtact gtcatttcac tcctgctttc taagaacgtt ccattacttg
    16621 acaaacaacg attgctttgg aaacgatggt gttttcaata aacacttcga tatccagttt
    16681 agcggaacgt ctgccgattt ccaatacacg tggacgaatg tctaattcgc tttccaattg
    16741 gatcagccgc aaataatgta ggttcacttg ttcaatcaag atatttcgtt tttggttcat
    16801 caccattgtt ttttgtgcga catcagaaat gatttcgcta agtacgccaa aagaaatggt
    16861 tccaacgcca ttgaccattt gtggtgctac tacgaatttg aacttagggt tttccaaacg
    16921 atttccttca cggtcagtat caatcgtatg gatatttcca gatatctgat ccgaaatcgt
    16981 atctgcaatc tgcggttgtc gctggacaag ctgcatcgct ttcattacat cttgccgggt
    17041 aatcaagcct tccaatgtca agtcatcgga aacgaccggc atgacttcta gcccatccca
    17101 gatcatttga tggctaacag aggcaatact cattgttttc tttacactga ttggttcttt
    17161 cgtcatcacc cgatcgatga tctggtttgg cgatttccct aaaacatctt ttgccgtaac
    17221 aatccccatc aaacgatgat ggcggttgac gactggaaaa cgcgaatgct gcgtttcttc
    17281 tgacaaacgc tgataatcag aaatcgtatc tgtctgctgc aaataatgag tcttttcagc
    17341 tggcatatag atatcactga caagcatgat atcttttttg atcaattggt cgcttaacgc
    17401 tcggttgatc atggttgcga ctgtaaatgt atcataagag gtgcgtaaga tcggcatttc
    17461 aagatcatca gctagctttg caatctcagc agtcgtattg aatcctcctg tgatcaatac
    17521 tgcggcgcca tcttccaatg ctaatttttg gacttccgcc cggttgccga cgatcattag
    17581 tgatcctggc gtaatatagc gttccatggc atgtttagtc atcgcgccga tgacgaattt
    17641 attcaagacc ttgtctaatc ctgccgcccc gcctaaaacg tccccttcaa tgatcctgac
    17701 gacctcgcca aatgtcaaac gttcaatatg tttttttaat ttgcgttcga tacggatggt
    17761 ccctactcgt tgaattgttg aaactagtcc gacattttct gcatctttga ttgcacggta
    17821 cgctgttcct tcactgacat tcaattcttt tgcgatgctt cttacagaaa ttcgattgcc
    17881 aacaggtaaa ctttcaatat attctaaaat caaatcatgt tttgtcgcca ttttctgcct
    17941 acacttccag cgtttccgag acgctcatta tttttccttc actatcttcc agtaactcta
    18001 caaaagattc gggatcttgt tcaatgacag ggaaagtatt gtaatggatt ggaactactt
    18061 tcttggcacg aattgtttgc gcagcctttg ctgcatcttc tggccccatc gtatagttat
    18121 cgccaatcgg caagaaagcg acatcaatat cgaattcttc tgcaagcaat cctagatcac
    18181 tgaaataagc agtatcccct gcatgataaa tcgtttttcc ttctgcttga ataataattc
    18241 cgctaggctc gcccatatac tgaacgatac cgtctacttc atagcttgaa ctatgctgtg
    18301 catgtacgaa ttttaccacg ccgaatggaa acgcatgttt tccaccgata ttcataccga
    18361 atgaatctac tcctctgctg ttcgcataat cagcaatctc aacaatactg ataattgtgg
    18421 cttgattttc ttttgcaata ggaatcatat ctcctatgtg gtcactatga ccatgagtaa
    18481 tcagcaacca atctgtcttt acttcttctg cagttaagtc tgtcaatgga tttcctgtaa
    18541 taaacgggtc aatcatcacg cgttggccat tttccatttc aattgttaaa caggaatgtc
    18601 catgatacgt aattttcata ttttacacct cgcgttttct ctgtatcttt tttattaaaa
    18661 acgatttcat cacttgcaca tcaatgaaaa aatatagctg ctaaatatat tttcattgga
    18721 ttgatagtac agtatgtaca aagcaattgt aacagataat acaggatgca ccaagtacca
    18781 agcagatata atctcaagtt tgaggtctct gctctgctta gcttctcttc tcgtccctta
    18841 actaattaaa tcagcgtaaa taggatggac gatcttttct aaatcggcta cttttatccg
    18901 aaggcttctc ccgatttcac cgcttgacac aattacttct tctttttctt ttaaagaact
    18961 gtctgctaaa aaacaataat caggatgtgc gagttttatt ccaatagggg tgtttgctcc
    19021 gtggggatat cctgtatgca ttaatacaaa atccattcca ggaaaaccaa ctttacgatc
    19081 tttcgtcact tgtctcgttt ttttgtaatc taagtgatcg ttcaaaggaa tcaatgcgat
    19141 gatcacccca gttttgtttc ctttcactac gagagtttta catatcaaag ctggatcaat
    19201 ccctcttttt tgtagttctt ctgtgaaaga ttcttctacc acatgactaa aatcaagtgg
    19261 ttgatagctg atcttttgtt cttctaaata acgttcgact tttgtttgac tcatcattga
    19321 ttcactccct ttattctatc gtttattata aagcgaggga tgctgtattc aaaaattatt
    19381 tgtttttcgc aaaaaaagag caagacgagc actctataga gaggttcatc ttactctttt
    19441 tgtttatttt ttatttcgac gttgagtagc ttccataatg actggcaaga tcactgggcg
    19501 gcgttttgtt tgttcaaata agtaacggcc taattgatct cggatgtctt gctttaattt
    19561 gctccactca aaatcattgc tttctaaatg tttctcaacg attgtttcaa tcatattgct
    19621 gctttctttc atcagatcac ggctagcttt tacgtaaacg aatccccgtg aagtaatttg
    19681 aggttttgaa atgattttct tttctcgccg actgatagtg acaaccgcta cgaaaatccc
    19741 gtcttctgac aagatttttc gatcacgtaa aacaatgttt ccgatatcac ctatgcctaa
    19801 tccatcaatc attacatttt ctgcacttgt agttcctgca acactcatac gcttgttctt
    19861 atattccagc acgtctccgc gtccagtaat atagatattt ttgtatggca tccctactgc
    19921 atgagccaaa tctgcatgag cagctaattc acgatactct ccttgaactg gcacaaaata
    19981 tgttggtttc atcaaattga tcattaattg caagtcatta ggattggcat ggccagaaac
    20041 gcgcatattc tcagagatca acttcactgt tccaccagct cgataaatga tatcttccgt
    20101 tttcgccacg atggtttcca tcgcgatgct tggcgtagta gtgatataaa ccaaatctcc
    20161 ttcttcaatc ttgacattac ggtgtgtacc gtttgccatt ttttgcagag acttgatggg
    20221 ttcacccatt cggccagttt ctaaaatgat cagttcttct ggatcatagt ttttcatgtc
    20281 tttcaacgta acaagcagat cttcacttgg taattgaagt ttttctaaac gcatcgctgt
    20341 gcgaataatt cgttcgaaat cttgaccagt caagacaact tttcgttgtg ctttttctgc
    20401 tgcatccaat acttgttgca ctcgttgcaa attacttgct acacaagcga cgatgatacg
    20461 cccttcccaa tatttgatcg tatcaaatac ttcatctgct atttgggctt cagaagctac
    20521 aggctgagga ttttctgcat ttgccgattc acttagtaag gctaagactc cttctttgcc
    20581 gatttccgcc aatcgtgcat aatctgtttg atacattggg atagccgttt ggtcaaattt
    20641 aaaatctccc gtataaacga tactaccttc ttttgttttc agattgatcc ccactgaatc
    20701 tggtatcgta tgagtggtac ggaaaaagct cactactgtt tcaccaaagt ctatttctgt
    20761 atgctcatct accacatgga aatccttaaa tcctttggct gcttcattac gactgacatt
    20821 caattttgct aactcgatcg tcaactctgt cccgaatact ggtacagaaa tttttgacaa
    20881 caagtaagga agtgccccga tcgcatctgc atgtccatgt gttaaaaaga caccagctac
    20941 acgatcaatg ttttcttcta aatacgtaaa atcagggatt acgacgtcga tccccaacaa
    21001 ctcattttca ggatatttta gcccacaatc taaaataaaa atatcctcgc ccacttctgc
    21061 gacatacatg ttttttccat tttcacgaac gccgcctaat gggataattt taatggtact
    21121 cacaaacttc acctcttctt atgacatcca agtttaaaga tgtcgatttt cttctttatc
    21181 taaatatatg agccattcca atgaagcata tgcaaaatca ctatttctca acttatggag
    21241 aatcagcagg aaaattacca gcacatccgc gttctgacag ttggatgggc attttatttt
    21301 ggatatactt cattaacaaa atggatcttt tgcgaaacgc tatagccaga atacttatat
    21361 aagtattcag ctgttcccca ttattataca acaaataaac taaatcgcta gtatcagcaa
    21421 ttgttagaac aactatgcac gtaaaaatcc ctctgtagca tctaggattt cttttgctat
    21481 tcctctttca tttcttcttc gtcggtttga aacatacttt tttcatttta ccacattttt
    21541 tgcaataact atagtgactt tttatatata cagaacctca tgttgatggt gtgcaaacag
    21601 cataagattt aagctagctt taagataggt tgtctattat caaatgcatc tactaggagg
    21661 gatgcaaaat atgaatttta tgaaacgtgc atggaaaagt acaaaagtaa aatggggacg
    21721 ttcattgtta ttgtttgccg tgttcacagc cattcttatt tttgtgctag ctggcctcac
    21781 gattcgaagc gctgcagaag aagcagcgaa tcaagcacaa aaagaagttg gagcaacagt
    21841 gactttaagt gccaatcgag aagcttcatt taaaaaacag gaacaaactt cttcaagcgg
    21901 ttctaccact gaagaacgtc cagatccagg cagtttttct cttacaccag tttccttaac
    21961 ggacgcggaa aaaatagctg aattagataa tgtaaagagt tattcctttg aaagttccgc
    22021 ctcagctatg gcaaaagatg gaataactgc catcactagt tctgattcaa gtgaagacag
    22081 ttcagaaact actgatgcag gcggtgaacc tgctggagga atgggcggga atccgcaaat
    22141 gactcaagca gactttcaaa tcagtggtgt gtcagcaact gcacaaaata gtaatttttc
    22201 tgatggaaca gcaaaaatca cggatggtga aggaatagat gacagcgaca aaggcacaaa
    22261 caatgtagtc attgattcta ctctagcaga aacaaatgac ctggcagtcg gagatacttt
    22321 cacaatcacg agtactgaag atgaagatac cacttatgaa atgacgatca aaggaattta
    22381 tgaatccagc gaaacaagcg gcagtatggg tatgaaattc aacttcatga atccgtcgaa
    22441 tacactttac acttcttaca cttttgcaaa tgaactaaat gggacatctg acgataacac
    22501 gatagattct gctatttaca ctctatctga tccagataaa atggatgagt ttctcaaaga
    22561 agctgagaag ctgatcgaca cagatacatt tagcttacaa tcaaatgatt ccatgtatca
    22621 gtcaatgttg gaacctttaa acaacgtggc aagtttttct aaaaatgttg ttttactagt
    22681 ggctgtagcc ggtattatca tacttactct gatcattatg attaccattc gggagcgtcg
    22741 gcatgagtta ggcgtcttgc tatctttagg agaatccaga agtaaaatca ttctccaatt
    22801 gtttaccgaa gtggctattt gtatgattct tgcattagga gttgcttcgc ttagcggaaa
    22861 cgtcgtcgcg aatgccgtgg gacagcagct acttgaccaa cagacagaaa ccacgacaca
    22921 aaaccagcaa gctcctggcg gtggacaaat gccaggtaac agacaagaac aaggcggtca
    22981 acgcgatggt caacaaggaa ccacgaataa tccatttgct gtttctgatc aagtcagcga
    23041 attggaaatc aatgtcaaac ctgcacaatt gggactatta gccggagtag catttggaat
    23101 cagtctgttt tctgtctttt tagcttctat agggatatta agattaaacc caagaaaaat
    23161 cctattaaac taaaggagga atttgatgtt acggacagaa caattgggat tttggtatca
    23221 agatccgcaa gatgcgcttt ataaagatgt agaactgaat ttcgaagctg gaaaaatgta
    23281 cgctatttta ggagcaagtg gttctggaaa aacgactttt ctttcgctta tttctggact
    23341 agatgcacca aaagaaggaa aggtatacta tcaagatcgt tcactgaaca aaatcgggtt
    23401 aagaagttat cgtaaaaatg atgtctcgat tattttccaa gcttacaact tgcttcctta
    23461 tttatcggct ctagacaacg tcatagcagc aatggaaatc actaagacca agcaagagaa
    23521 ttatcgacaa gttgctttag atcgactggc tcgtgtagga attgatgaaa tattagcaaa
    23581 aaaacaggtg accaaacttt cgggaggtca gcagcagcga gtcgctatcg ttcgtgctat
    23641 ctgctgtgaa catcaactaa tcgtagcgga tgagcctact ggaaatctag atgaaacaac
    23701 ttcacaagat atcgtcaaac ttttccaaga aatcgcccat gaacaaaatc gctgtattat
    23761 catcgtgact catgagattg aagtagcaaa aagctgtgat gaagtctttg aactaaaaaa
    23821 taaaaaattt caaaaaatcg aagtttaaaa aacaggataa tgatcaaat
//
LOCUS       DAYYGO010000032        23829 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000032 DAYYGO010000000
VERSION     DAYYGO010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.032"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(54..788)
                     /locus_tag="ACHA5T_001071"
     CDS             complement(54..788)
                     /locus_tag="ACHA5T_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFM8886661.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1115..1525
                     /locus_tag="ACHA5T_001072"
                     /pseudo
     CDS             1115..1525
                     /locus_tag="ACHA5T_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
     gene            1518..2219
                     /locus_tag="ACHA5T_001073"
     CDS             1518..2219
                     /locus_tag="ACHA5T_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFM8886662.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2219..3805
                     /locus_tag="ACHA5T_001074"
     CDS             2219..3805
                     /locus_tag="ACHA5T_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8886663.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3824..4045
                     /locus_tag="ACHA5T_001075"
     CDS             3824..4045
                     /locus_tag="ACHA5T_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFM8886664.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4042..4803
                     /locus_tag="ACHA5T_001076"
     CDS             4042..4803
                     /locus_tag="ACHA5T_001076"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFM8886665.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5183..5692
                     /locus_tag="ACHA5T_001077"
     CDS             5183..5692
                     /locus_tag="ACHA5T_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFM8886666.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5767..6306)
                     /locus_tag="ACHA5T_001078"
     CDS             complement(5767..6306)
                     /locus_tag="ACHA5T_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFM8886667.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="ACHA5T_001079"
     CDS             complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="ACHA5T_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFM8886668.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7319..8170
                     /locus_tag="ACHA5T_001080"
     CDS             7319..8170
                     /locus_tag="ACHA5T_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFM8886669.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8198..8833
                     /locus_tag="ACHA5T_001081"
     CDS             8198..8833
                     /locus_tag="ACHA5T_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFM8886670.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8853..9626
                     /locus_tag="ACHA5T_001082"
     CDS             8853..9626
                     /locus_tag="ACHA5T_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8886671.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10034..10831
                     /locus_tag="ACHA5T_001083"
     CDS             10034..10831
                     /locus_tag="ACHA5T_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFM8886672.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10809..11222
                     /locus_tag="ACHA5T_001084"
     CDS             10809..11222
                     /locus_tag="ACHA5T_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM8886673.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11206..13851
                     /gene="mutS"
                     /locus_tag="ACHA5T_001085"
     CDS             11206..13851
                     /gene="mutS"
                     /locus_tag="ACHA5T_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFM8886674.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13869..15977
                     /gene="mutL"
                     /locus_tag="ACHA5T_001086"
     CDS             13869..15977
                     /gene="mutL"
                     /locus_tag="ACHA5T_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFM8886675.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRQTGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15999..16559
                     /locus_tag="ACHA5T_001087"
     CDS             15999..16559
                     /locus_tag="ACHA5T_001087"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFM8886676.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16644..18632)
                     /gene="treP"
                     /locus_tag="ACHA5T_001088"
     CDS             complement(16644..18632)
                     /gene="treP"
                     /locus_tag="ACHA5T_001088"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFM8886677.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18855..19568
                     /gene="treR"
                     /locus_tag="ACHA5T_001089"
     CDS             18855..19568
                     /gene="treR"
                     /locus_tag="ACHA5T_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFM8886678.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19645..20091
                     /gene="msrB"
                     /locus_tag="ACHA5T_001090"
     CDS             19645..20091
                     /gene="msrB"
                     /locus_tag="ACHA5T_001090"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFM8886679.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20262..20864
                     /gene="ruvA"
                     /locus_tag="ACHA5T_001091"
     CDS             20262..20864
                     /gene="ruvA"
                     /locus_tag="ACHA5T_001091"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFM8886680.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20877..21878
                     /gene="ruvB"
                     /locus_tag="ACHA5T_001092"
     CDS             20877..21878
                     /gene="ruvB"
                     /locus_tag="ACHA5T_001092"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFM8886681.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21907..22485
                     /locus_tag="ACHA5T_001093"
     CDS             21907..22485
                     /locus_tag="ACHA5T_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8886682.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22537..23019
                     /locus_tag="ACHA5T_001094"
     CDS             22537..23019
                     /locus_tag="ACHA5T_001094"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFM8886683.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23136..23510
                     /locus_tag="ACHA5T_001095"
     CDS             23136..23510
                     /locus_tag="ACHA5T_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886684.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(23623..>23829)
                     /locus_tag="ACHA5T_001096"
     CDS             complement(23623..>23829)
                     /locus_tag="ACHA5T_001096"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886685.1"
                     /translation="NLTTFENFRHKIFTTNDYYISIPTNNPNNTFSFLTPYPASSPAS
                     RTFFYQKNLYHPIDSTNQPNAVML"
BASE COUNT         7781 a         4286 c         5114 g         6648 t
ORIGIN      
        1 taaaaaaact tggtacgcaa tcgctgtacc aagttttttt atttagcttt tgattaagcc
       61 ataaataata atttatataa gaatactgct acgacaccag cacagatagg tgctacaact
      121 ggtacccatg catatcccca ttgagaagaa cctttatgtt tcaatggaag aagtgcgtgt
      181 acaagacgtg gagcaaagtc gcgggctggg tttagtccag gacctgtagg accaccaagt
      241 gaagcaacta atgtccatac aaggaaacca agtgctaagt gagctgtacc aagattatct
      301 tggaagaatg gtgcttttgt aatacctagg gcaccaaaga ataaaacgaa tgaaccaaag
      361 aattcattga cgaaaccatt gaatttagaa ccaacgctgt tgattgttga gaatgtacct
      421 aacacatggt ttacgtcttc tgtttggtcg tagtaaggtt tgtaacaagc tactacgatc
      481 aattgtccaa tcgctgcacc aatcaattgt gcaatgatat aaggaacaac ttcagcccaa
      541 gggaatagac cattagatgc taaagcaatt gtaaatgctg ggttgatgtg gttaccgctg
      601 attccaccaa aaatcattgc tggcatcata accccgaatc catacccgac agcaatcagc
      661 atccagtcac tgccatatcc ttttgtacct tttaaatcta cgtttgctac agcaccgtta
      721 cctagaacga ttagtaatgc tgtaccaatc atttccgccg caagacgggt cattagtgag
      781 taatccataa atacctccaa aagttataaa aatgcaaaaa aaaaagaaac atcaacacga
      841 aaagcatcgg tgcagtgttt cgtgtcggaa ggatttctgc agtcttaaca aatgtgaata
      901 aaaaaatgaa ttccattact tataagaaac cagaccaaca gtaaaactcc aactcttttt
      961 aaaagttctc cacaaatcac caagcttaac tataaatata ccaaaaaaaa aacgtttttt
     1021 ttctcaaaaa ttgtatctta gaaacagcat gttttgaaac tttcagaaaa ggtttgttag
     1081 taaaatgaaa gctatacgta aggagtgttg tttgatgaaa ttttttcaag agtttttcat
     1141 tattattttc ttttctgtat taggagaagg catacggatt tttgctcatt tacctattcc
     1201 tggaagtatc ttaggtattc tttttttatt tttagcattt gaaatgcatc ttgttgatcc
     1261 tgaaaaaatc ggagcaactg gtgatttttt attgaataat ctagccattt tatttgttcc
     1321 tgctggtgta ggtttattgg aatattttga tgatattgct tcgatatggc ctgttttact
     1381 gggagcagtc gtcgtgtgtt ccatcgtcac gatggttgca gcgggaaaaa cggcataagg
     1441 tgtagaagct ttgctgggct atgtccgtaa gaaaaaagca gaaagggtat ctgtaaacga
     1501 aatggaggag aatcaaattg actgagtttt ttgtgaatcc attatttggc ttaagtttga
     1561 ctttaggagt gtacgtatta tctgacaaat ttttgcggaa atataaactg cctgttttga
     1621 atcctctcgt attatcgatt gttgttatta tcttattttt gcatttttct gggatcagct
     1681 ataaagacta taatatcggg ggcaattttt tgactatgct gattactcct gcgacagtgg
     1741 cacttgccat ccctctttat aaaaacttcc gtttactgaa agagaatttt tatcctgtaa
     1801 tggctgcgat tttagcagga atagtcgcaa attgcttggt tagtgtaggg ttcggttact
     1861 tcttctctct tcataaagag atggtgatct cattactacc taaatctgta acgacggcta
     1921 tttctgtcga tttatctcat acgatgggcg gggtgaatgc cgtgacactt gctattgttg
     1981 tctctacagg tatttttggt tcgctgatcg ccactcatat tttccgccta ttcaagatcg
     2041 aaagtccagt tgctcgcggt gtggcgttag gaagtacttc tcatgctatt ggtacagcaa
     2101 aagcaatcga gattggtgaa atagaaggaa tcatttccgg acttgcgata tgcgtcaacg
     2161 gaatattgac cgttttattg ctaccgttat tctttcagcc ttttgctggc ttgttttgat
     2221 gcgaaaacca ctattttatg ctacaataac caaaacgttc gagcataaaa aaggagggaa
     2281 agacgtgtat ttgtcttccg agtatcttaa acaatttgat aaagatctat attataagat
     2341 tctcctccta gaaagttttg aggatcaagc ttggcatacc gctgcacaat tagcgcaagt
     2401 tgttcagctg gatgcccgca gcgtgtcgaa atacctgaac gagctgtcta aaaactatca
     2461 gcagtttagc ggtaagacac accctttgtt tacaaaaaat caccgttctg gttataactt
     2521 ttatgatacg ctcgattcaa ttgaacatga gcgttttttg atctatctcg tgcaaagtac
     2581 gctgaaattc cagttgctgc acgacatttt ttttgaagaa ttccatacaa tgtaccaatt
     2641 tgcgcaaaag cattacatta gtgaatctac tgctcatcga aaaatcaatg aatggaaaca
     2701 gcaactgcaa acttacggaa ttcggctgca acgaggtaca tatatcgctc aaggcgaaga
     2761 agaaatcatt cgactttact tgcatatgac attctggcag ttgtttcgag ggaaaatctg
     2821 gccatttgaa acgatttctc aaatggacgt aaagaatatg gcagaacata tcatggcttt
     2881 ttttaatgtt cgtctgaacg agatcaaaaa aagacgattg gaatatatgc taggtgcctt
     2941 ttttttaaga aaatcacaga agcattatgt tgtattgaat gagaaaaaaa gacggctgat
     3001 ttctgacaat ctgttgttcc agcgattttg ccaggtcatg gagccggtat ttcctaatta
     3061 ttttcaagta gaagacgagt taggcgcatt atttcttgta ctgatgactc gtgaagaata
     3121 ttatagtgat cctaagatac ggaagaaaat ttttgatttt catcaacaag caaaaacacc
     3181 gccctttaca gccctttcag aagcaaaagc cgctctttct ctttatcagg aagagcaggg
     3241 gctgccagca gaaaatctaa ccttcgaggc agaaaactat ttattcagca gtcatttttt
     3301 cgcctatctt tttcctaatg cgaaagaaac gattgatggc aatagtagtg attttattaa
     3361 tcatttagtt attgaaaaca aggaattgaa acaatggctc gtacattttt ttgaaagtag
     3421 acacaaacat cccaatcatc tggcatttaa aaatcatgcg tttttgatgg ggcggtatct
     3481 gactgtgttt aaaacattag gagcctttac tccacagctt ccaaagatca cgatcctttt
     3541 aatgacagat ttcccattat ttgaagagca actgttagag gagggattac ggacattttt
     3601 ccgtaatgag tatcagctga tttttctgcc aacagattat cgggggagag aagttgatct
     3661 tctgatttcg acaagtaaag tccaccgcaa accatgggcg gatcttgact attttattgt
     3721 gactgaagaa ctaaaattaa ttgattacat ccaactatct caaaaatttg aaatgattca
     3781 aaaacaaaaa ctattaaaac aataacaaag aaggagaaaa aagatgacat ttgaagaaat
     3841 tttaccagaa ttgaaaaaag gtgcaaagat tattcgtaaa ggatggagtg gatttgaact
     3901 ttatgttgtt ctcgtatccg atgatgaatt tgatggctgt ccggtgacgc cgtacttatt
     3961 gatcaaaaca agtgacgaag gtttttccag cttcgctcct acagtttgtg atattttagc
     4021 agatgattgg gcgatcgtag aatgaaaaac cagtatccag atctagctaa acaggttgta
     4081 tttatcacag gagctgcttc tggaattggt gctgcgcaag cccgagcgtt tcttgaacaa
     4141 gatgcttttg tatttggatt agatcaaaag ttcggtaaaa tggaagaaat ttctttgcga
     4201 tttcctgaaa cttttgccta cgcactaggt gatgttcgac aaatgaaaga actccaacaa
     4261 gcggtatcga aatgccaaaa tcattttggt gaagttacga ttttactgaa tacagcgggg
     4321 atattggatg cctacaaacc cttgatggaa actgacgaat cgctgtggga tctgatttat
     4381 gagacgaatg tgaaaagcat gtatcagctg acaaaaatca tccttcctga tatgatccgc
     4441 caaaaaagcg gaacgatcat caatatggct tcgattgctg gcttgattgc tggtggaggt
     4501 gggatcgctt atacaagcgc gaaacatgcg attgtcggat ttaccaaaca actcgctctg
     4561 gattacgcag gacagggtat ccgagtaaaa ggaattgctc caggagccat ccaaacacca
     4621 atgaatgcgg ctgattttga agggaaaggt gagatggcag agtgggtggc aaatgaaaca
     4681 ccagtcaagc gatgggcaca gccggaagaa gtagctgagc tgacgttatt tcttgcctct
     4741 cctcaagcaa gctacataca aggaacgatc atcccaatcg acggcggatg gttgttaaaa
     4801 taaccttgtg tttttttcaa gcttctgtat gataaaacta aagagagaaa caacggttga
     4861 tgataaaaaa agtcgatgac tgttcccgac attcgatgcc gaacagccag ttcaaaaaat
     4921 tctttttaat aatcgttaat attcgaaact aagcggcttt tcacgacctc ttgatataaa
     4981 cgatattcaa aagcttgcaa ctgcggtaac aagtccagcc aaatgaaaaa tataaggagc
     5041 tatttttatt tgaccattct tattacttgt tgcagggcag ctttttcact accttctgcg
     5101 acacttcagt tggaattttc ttcccatcct ttggtcgcgg tcattaccaa gataaaatta
     5161 tcttgcaaag gagaaataga acatggaaaa aaataatacc cgttcagcca aacgctggac
     5221 aacgacagat gtgactcgga tggcgctagt cactggttta tatgtggcag tgacgctggt
     5281 tttgtctgtc tttagctttg gagtgattca gattcgtctc tcggagatgt tcaactatct
     5341 gtccttgtat aacaaaagat acatcgtcgc ggtaacagcg ggagtagctt tggctaatat
     5401 tgcttctcct cttggactga tagatgttgc agtaggaagc gtcagcacgc tgcttgtatt
     5461 gcttatcaac tataaaatca ctagtcgcat caaaaacatg aaaatcaaaa tggccgtgac
     5521 agcatgcgta tttgcttttt ccatgtttac agtagctggt cagctaacga ttttatatca
     5581 actacctttt ttcttgaatt ggtgggtgat tgctttagga gaattggcat caatgattgt
     5641 cggtgggata attatttatt ggatcggtaa aaaagtggat ttgtcaaaat aaagatctgt
     5701 ttaatttaaa atagggcagc atggaagaga ctgaatgttg agtcttcttc catgctgctt
     5761 ttttgattaa tttgtatgaa gaaatcgatt tagccgtttt ttcatcaaga taccgatata
     5821 agctgctcca gttgccttga ttgcttccgg cagtaaaaac ggcagtagtc cactggcaaa
     5881 tgcagttgta aaagtaagat caccacttat tttcagccag atcgttccgc agatcaatgc
     5941 agcaagagag cccattagat tggcaaagat tgcatataag taatgagcat ccgttctttc
     6001 gatcatccaa ccagttatcc acgtttgtaa gagaaatcca atcaaatatc cgccagttgg
     6061 cccaaataaa acagctatac ctccagtcat ccctgaaaat acaggaagac caatcaagcc
     6121 aagtaaaaga taaactccaa cagatatcaa tcctagtttt cttccgagaa aagttgctgt
     6181 taatccaata gcaaatgttt gccctgtcaa aggaaccaaa ccaaaaggaa tggtcatctg
     6241 agaaaggaca gaaataatcg cggcaaattc cgcagcaagt atcaattctt tcaatgttcg
     6301 gttcataacg ccctcttttc gttaacttaa tatttttatt tggttaacga aattgtaaaa
     6361 caaagtgatt actttgtaaa tagaaaatat aaaaaaagac cagctatcca atagatagcg
     6421 gtctttttca agaaagcttt tgaaattata agctttggtt gtagtattca acgacaagag
     6481 cttcgtcgat ttctggatat agttcgtcac gttctggtaa acgagtcaat gaaccttcca
     6541 atttttcagc gtcaaagctt acgaatgctg ggcgtccaac agttgcttct acagcttctt
     6601 ttactgttac gatgttttgt gatttttcac gaactgagat cacttgaccc acttctacgt
     6661 ggtatgaagg gatatcaacg cgtttgccat caactaaaat gtgaccatgg ttaactaatt
     6721 gacgtgcttg acgacgagtc gttgcaagtc ctaaacggta aacaacattg tctaaacgtt
     6781 gttctagtaa gatcatgaag ttaacaccat gtttaccttc tttgattttg ctagctttta
     6841 cgaacaagtt acggaattga cgttcgttca ttccgtacat gtgacgaagt ttttgttttt
     6901 cagttaattg catgccgtat tctgattgtt tgccacggct attaggtcca tgttgacctg
     6961 gtgcgtaagg gcgacgtgct aattcttttc ctgtgcctga caatgagatg cctaagcgac
     7021 gagaaatttt ccatgatggt cctgtataac gagacattaa aatttcctcc aatgaatata
     7081 agtttttgga gtaaaataat ccgttgaaaa attcataatc atatagtttg ttcttcaatc
     7141 ttcacctttg cagccgcggt tacacaatcg aacccgagac ggggcaacaa actagggatg
     7201 agttattatt tttctgctgc attattttac acaaattaca gtatatgata gatcatccac
     7261 gaatgtcaag ataattagta cataaaaacg ctttacaaac ctaggctgtc tttataaaat
     7321 gagagggtat gtgcaaatgg aaaaaagcga aggaagttta aggagaaaat caatgaatcc
     7381 ggtatcacca ttatttgaga aaatcgatgc ttcaattgaa aagaaggcga atctttttga
     7441 aaacagcttt tcacgttacg ctgttcgagc ggttctcgcc tgtttgtttt tgactttagg
     7501 aacggctgtg gcatttgcga tcgcgatgaa gggggaagat atcgcgcatg gtctaggaaa
     7561 gattttatac gcatttatgt tcagctgggg tctggtcatg attctataca tgaacgccga
     7621 attaggaaca tcgaatatgc tttatatgac cgtgggtgtt tttcggcaga gacttgcttt
     7681 aagtaaggca gccaagattc tatttgcctg tatttttttc aatatgattg gcggtatatt
     7741 atgtggattt ctaatttcta tgactacgcc atttcaagat ttaacgttgg acaactttat
     7801 gttagaatca atagcagtaa aactagcaaa accttcagca acgattttgg tggaagggat
     7861 gtttgcgaat atcgttgtca ataccgctgt actgatcagc atgcggatga aagaggatgt
     7921 cggaaaagtg atcacagtcg tattcatcat tttcattttt gcttttttag gatatgaaca
     7981 cgtgatagcg aattttcctg cctttacgct tacttacttt gcttctcacg gacagatgga
     8041 tggaatgaca gtcagcaatg tgttacataa tttgtttttt gctttggcag gaaattatat
     8101 tggtggcggt ttagtaatgg gactgggata tgcttggctg aatcagtcaa aatcatccta
     8161 tgtggactaa aaagtcgttc attaaagggg aagtgccatg gaagttttag tttttccaaa
     8221 aaatgaggaa aaagcgttac aaatggaaaa gtacatgaaa aatctttttt cctttgctgg
     8281 agtacctaag ccggaacgcg ctcttttaca acaaccgtta ctggatgaga gcaaaaattt
     8341 atctatttct gaagttttag cttttgttca ttattattat gaacaaatcg aaagagagta
     8401 tcagtatgta gcgatcgatt tagtccagaa aaacatcaaa cggcttacat ttgaagactt
     8461 agtcgtattg ttgccactag ttcagaaaaa agaatggtgg gacagcatcg actcttggag
     8521 aaaagtatac agcacatggt gtaaagccta cccagagcag cgtgaagaag tctttcaatt
     8581 gtttgaaaaa aaggaagatt tttggctacg tcgaatagca atcacgttgc aattaggtta
     8641 taaacagcaa acagacacag gactattgat ccgagcaata gacgaagatc gtcaaacaaa
     8701 ggaattcttt atccaaaaag caattggttg ggcattacgc gattatagta agaccgatgc
     8761 cgattgggta aaaagacagc tgacaacaga tttgagtaag ttagctgttc gagaggcaag
     8821 taagtattta taagaaagcg agacgacaag agatgagacg caaattattt gcttttgata
     8881 ttgatggaac gttgttagat tcaaacaaac aagctttaga tagcacaaga gaagcattgg
     8941 aaaaattacg caaagccggt catctggtca cgattgctac agggcggagc cgatttcatg
     9001 cgcaagagat cattcgcgat cttgatttta cgaactacat tttatgtaat ggagcggccg
     9061 gttttttaga ccatgaacaa gtatataaga atttactaga tcgcgaccag ttggatgcgt
     9121 tcgttcaaga agcgcatcat gagcagatcg atacagcttt tgtcagctta gacaatatca
     9181 agcgttcttc ttccaatgat gtaacggtga tggaagaagc gatgcattct ttcggtgctg
     9241 tcttgcctga attagatcag ttttttgcag aggaacaaga aatataccaa gcgttagcat
     9301 ttttcgatca aacatatgat gggaagttct cttcatatga taaaattcga tttgtccgct
     9361 ggcatgagaa cagtgtagat gtcgtaccta aaaatggttc taaagcagcg acaattctgc
     9421 acttagcaga acaagtaggc atccctcatg aagacatcat cagctttggc gatggacaaa
     9481 acgaccgtga aatgttgcgc atgtcaggaa ttgggatagc tatggggaat gctgtgccag
     9541 aagtacaagc agaagctaag atggtcactg atacaaacga tcaggatgga atttggaagg
     9601 cattgaaaga gttaagcgtg atttaaaaga ggttgtgaaa aagctgttct gcatcaagta
     9661 ataagcccag ccaaacaaaa atagctcctc ctatttttcg tttttctggg cttattaccg
     9721 cagatgcaag ctttcgaaca ccgtttaagg aagaggtcgt gacagaagtg gtcagtttca
     9781 agaaataaga agggatttac gaaaattgct tctcaaattt ttgtaaaatt acggcttatt
     9841 tctgaagaag tagatgaaac aacgtttatt cgggaaatgg gactgtgaca agatgtttgt
     9901 catagtccca tttttttatt ttatttttca aaactaagaa ataccaggaa agagttcctt
     9961 ttgtttgacc ttttctttct tattgaaaac aactataata tagagtgatg ttaaaagaga
    10021 agaggaattt agtgtgcggg tattgtttat tggtgatgta gtaggttcaa tgggacgaga
    10081 gatgattact gaatatcttc ctagattgaa gaagaagtac cgtccgcaag taacgattgt
    10141 aaatggtgag aatgccgctt ctggacgtgg aatcacagaa aaaatctata aaaaattttt
    10201 gcaagacggc gtagatgtcg tgacgatggg gaatcatacg tgggacaacc gcggaatctt
    10261 tgagtttgta aatgaagcta aaaagatggt tcgtccggca aattttccgg aagggacgcc
    10321 tggacaaggg atggtctttg tcaaagtgaa ccaagtcgaa ctggcagtga tcaacatgca
    10381 ggctcgttct tttatggtgg acttggatga tccttttcgt aaaatgaatg agctggtaga
    10441 agaagccaga aaacgtacgc cgatcatttt tgtagatttt catggagaaa caacaagtga
    10501 aaaacaagca atgggctggt ttttagatgg gaaagtttca gcagtggtcg gtacacatac
    10561 acacgttcag acaaatgatg cgcgaatttt gccaagagga acagcgtatc tgacagacgt
    10621 tgggatgaca ggtccctatg atggtatttt gggtatgcgc agagaacctg taatcgaaaa
    10681 gtttctgact gcattaccaa aacgttttga agttgtggaa caaggccgat cgattttaag
    10741 cggctgtatc ttggaattgg atgatacgac aggtcatgca aaagaaatcc agctgattca
    10801 gatcaacgat gatcgaccgt tcatggagta gaaagagagg cagttttttg cagcaggagc
    10861 cggaaattca aaaagaagta aggaaactga caaaactttt aagagagaat gaaacgatca
    10921 ttcgctacaa agaacttgaa gaaaagatcc agcaaaacca gtatttagct gagttgagag
    10981 aaaaaatcaa acaagcgcaa aaagatgctg ttcattttgc ccactatgac aaaccagcag
    11041 cagaaaaaga agcaatcaaa caagcggatc aattcatgca agaatttgat cagcatccgc
    11101 ttgttgtagc ttatagaaaa cagctgttgg aagcagatga tctgcttcat catttaacaa
    11161 ctatgataca agaagaaatc aatggacaaa tagaggagga aaaacatgcc tcaaaaaact
    11221 aaaaatactc ctatgatgga acaatatcta agcatcaagg cccaatacca agatgctttt
    11281 ttgttctatc gtttaggaga cttttatgaa ctattttacg aagatgcgat caaagcgtca
    11341 cagcttttgg aattaacgtt gacgagcaga aatcgaaatg cagaagaacc tattcctatg
    11401 tgcggtgtac ctcaccatgc agcacaagga tatatcgatg ccttgattga aaaaggctat
    11461 aaagtagcga tttgcgagca ggtagaagat ccaaagacga ctaaagggat ggtcaagcgc
    11521 gaagtgatcc agctagtcac tcctggtaca gtcatggaca gcaaaggatt gtcggcaaaa
    11581 gacaacaatt ttttaacagc tgtagttagt caaggaaatg aatttggctt tgcttatgcc
    11641 gatttaagta cgggagaatt gaagacggct cggttgcatg atgaagaagc tgttttaaat
    11701 gaagcctctg cattgcagac aaaagaactc gtattaggca gtgagatcac tgataccctt
    11761 cgtgaacagc taagcgggcg tttagggcta gtcttttctc aacaaaatga gatggaagaa
    11821 aacgcggaat ttagcttttt gacaagcgaa ctagtcgatc cgctggaaaa agaagcaacc
    11881 ggcaaactat tgacttattt atcggtgaca caaaagcgga gtttagctca tatccaaaaa
    11941 gcagtagaat accaaccaga tcattttttg aaaatggatt attattcaaa attcaaccta
    12001 gaattaagcc aatctattcg gactgggaaa aagcacggca ctcttctatg gttgttagat
    12061 gaaacaaaaa cagcaatggg tggccgttta ctaaaacaat ggctagaccg tccgcttatc
    12121 caggcaaaac aaattaaagc aagacaagaa atggtcgctt ctttgctaga cagctatttt
    12181 gaacgtatcg atttgcagtc agcattaacg aaagtctatg atctggaacg cctagcaggt
    12241 cgagtagctt tcggtaatgt caatgggcga gacttgatcc agctgaaaac ttctttagaa
    12301 caagtgccaa tgatcagagg gttgattgaa ggaatcgatc aaggtcaatg gcaaagtctg
    12361 ctttcagaaa tgcagccgat ggaccatctc gtccaattga tcgaccaagc tatccaagat
    12421 gatccgccgt tacaaataac ggaaggaaac atcatcaaag atggattcaa tgagcagctc
    12481 gatatgtacc gttctgctat gagaaacgga aagaaatggt tggcagaact tgaagcaaaa
    12541 gagcgtcaag aaacaggaat caagaactta aaaatcggat ttaaccgcgt tttcggttat
    12601 tatatagaaa ttacaaaagc aaatcttgga aatgcagaac tggagaagta tgaacgaaaa
    12661 caaactttag caaatgcaga gcgtttcatc acaccagaac taaaagaatt ggagacacaa
    12721 atcctcgaag cagaagaaaa atctgttgat ttagaatatc agttgttttt agctgtgaga
    12781 gaagaagtca aaaaggctat ccagccgcta caagtacttg caaaagcaat cagtgcagta
    12841 gatgtgctac aaagttttgc aacgatcagc gaacgctatc agtatgttcg gccagaactt
    12901 gtcagtgaca agcatcagct tctgatcaaa gacggacgac atcctgtagt agaaaaagta
    12961 ttaggacatc aggaatatat ccctaacagc gtagaaatgg cagaagacga aatgatcttg
    13021 ctgattacag gaccgaatat gtctggtaaa agtacgtata tgcgacagtt ggcattaact
    13081 gttctgatgg cgcaaatggg ctgtttcgtt ccggcaaaac aagcagtttt acctattttt
    13141 gatcggattt ttacccgaat tggtgcaagt gacgacttaa ttgctggaca aagtactttt
    13201 atggtagaga tgatggaagc caatcaagcc ctgcgttatg ctacgccaaa cagtttgatt
    13261 ttgtttgacg aactagggcg aggaacagca acctatgacg gaatggcgtt ggcccaagca
    13321 atcatcgaat atatccatcg tcatgtacaa gcaaaaacac tcttttctac tcattatcat
    13381 gaactgacgg tacttgagaa agaacttccg cagcttaaaa atgtccatgt tggagccgtt
    13441 gaaaaagacg gagaagttgt cttcttgcat aaaatgatgg atggacctgc ggataaaagc
    13501 tacggcatac atgtggcaaa gatcgcagga ttgccttcca atctactaga acgagcagca
    13561 gatatcctgc atacgctaga aaatggcgat aacggacacc atactgttcc agctgaagta
    13621 gaagagaaaa ctaaaacagc caaacaggag caaataaaac cacctgcaga aaaagtaaat
    13681 caagaccata tacaagaccg tgtaaaagaa gaaaaccaac agctttcttt attcagtgaa
    13741 ttatccgaaa atgaaacaga agtcattgaa tcactgaagc aaataaattt actagaaatg
    13801 acgccaatgg atgcactaaa cacattatat gaattacaaa aacaactgta ggaagaaggc
    13861 ggatatcgat ggccaaaatc caagaattat ctgaacgttt agctaatcag atcgctgcgg
    13921 gagaagtagt ggagcgacca gcttccgttg tgaaagaatt agtggaaaat gcaattgacg
    13981 ccggcagtac tcagattgat atcttagttg aagaagcggg actgcggacg atccaagtga
    14041 ttgataacgg agaaggcatt ttggcagacg acgtagagaa tgccttcaag cggcatgcga
    14101 caagtaagat ccataatcgc gacgatcttt ttcgcatacg aacgctgggc tttcgagggg
    14161 aagctttgcc aagtattgcc tctgtttctg aaatgactct ggaaacggcg aatcaggaag
    14221 aaaaacaagg cacctatctg tcgctgaaag gcggagaagt tattgagcat cgaccagcac
    14281 cactgcgtca aggaacgaag attaccgtca gcaatctgtt tttcaatacg ccagcccgct
    14341 tgaaatacgt gaaaacactg cacacagaat tagcgaacat cggggacatc gtcaatcgac
    14401 tggcattgag tcatccatcg attgcttttc gtttagtaca tgatggaaac aaaatgctgg
    14461 caactgctgg aaatggcgat ctgaaacaaa caattgcggg aatctacgga ttatctaccg
    14521 caaaaaaaat gctgaaaatc gaaggaaaag atctagattt tgagttattt ggctatgttt
    14581 cattgcctga ggtgactcgt gccagtagga attacttatc gacgatcatc aatggacgtt
    14641 ttatcaagaa cttttccttg aataaagcaa tcgttgcagg ttatgggtca aaattaatgg
    14701 tcggtcgttt ccctattgct gtattggaaa tcaaaatgga cccgctttta gtagatgtaa
    14761 atgttcatcc gacgaagcag gaagtccggc tgtcgaagga aaaagaacta acagagctaa
    14821 ttagtaacgc cattcaaaaa gcattgtctc aagaaaatct gattccaagt gcagcggata
    14881 atctacggtt taagaaaaaa gtacctgatc agcctaaaac agaacaaatc gaaatcgatt
    14941 tgtcctacgc ttttgaagaa gaaaaaccta aaaagccaag tagtttgaat tttgatcgac
    15001 agacaggtac ctttttcgta gaagaactaa agaacacatt tgttgacaga attgttgata
    15061 agtctgtgga aaatgttgaa aactattctg gtttttctga agaaaacacc acttatccag
    15121 aaaaaacagc gaaaacggtg gataagtttg gtgaaaatgt tgataacttt gtagaaaaaa
    15181 cagtggaaaa atctgaaaag aaagaatttc ccattgtgga taacatggag aaaacaaatg
    15241 aagaacaagc agataaatcc gtagaagagg atgaaaagat ttacgaggaa gaattgacac
    15301 tccatcctga atttgatttc actcaaggaa acgacaaaac attagaacaa gccatcaaga
    15361 aattagaaac ggaaaaacca gtacaacgtt ttcctcagtt ggaatatttt ggacagatgc
    15421 acggaacgta tctttttgcc caaagtagcg atgggttgta catcattgat cagcatgctg
    15481 cccaagagcg cattaagtat gaatatttcc gggaaaaaat tggggaagta tctgatgatc
    15541 tgcaagaatt gcttgttcca tttgtgttag actatccaaa cagtgatgct ttgaaattaa
    15601 aagagcaaaa acacttgcta gaagatgcag ggatttattt agaagaattt ggtcaaaaca
    15661 gctttattgt acgggcccat cctacttggt atcccaatgg agaagaagaa tcgattattc
    15721 gggagatgat cgatatgctg ctgacaactg gaagtgtgag tgtaaaaaaa ttccgtgaag
    15781 caacagcaat catgatgagt tgcaaacgat cgattaaagc caaccattat ttgaatgaag
    15841 cacaagcacg tgtgctgctg aaggatctgg cacgatgcga aaatcctttc aattgtcccc
    15901 atggccgtcc agtattgatt cactttacca attcggatat ggaaaggatg ttcaaacgaa
    15961 tccaagaccc gcattaagtc aaagagaaga gggaaaacat gaagattgta ttagcatccc
    16021 aatcaccaag aagaaaagag ttattggctc atatcgtttc agaattcgag attgaacctg
    16081 ctgatatcga tgaaacgcca tttccagacg aacagcccgt cgattatgtt cgtagaatgg
    16141 ctgaagaaaa agcacgaact gtctgggaac aagatgaaaa agaagaccat ttagtgattg
    16201 ccagtgacac gactgttgtt ctcaatcaag agatcatggg taaaccagaa gatctggctg
    16261 aggctgaatt catgctgaaa aaactaagcg gggaaacaca tactgtatat acagctgttg
    16321 ttttaaaaac aaaggaacga gaagaacgga tactagcaga agcacatgtc actttttatc
    16381 ctttgacaga cgaagagatc aaacgctact tggagacggg ggattatgcg gataaggcag
    16441 gggcatacgg catccaaaat gctgcgagtg tattcgtcaa agaaatttct ggagattatt
    16501 acagtatcgt aggatttccg attggtgctg tcaatcaggc actgaaaaca ttcatttaaa
    16561 caaaaaagcg gactatctga ttttcgattg aaaatccatg atagcccgtt ttttttattg
    16621 aaaagattac ttatctactc tgactaagat tttacttcag ctttaccgac tgcttcgccc
    16681 gggcgaacaa gtcctgattg gtcaagagaa agtcctgcaa ctttttcatt attggtcaaa
    16741 gcaacaataa tgtcagtttt cttgccagct tgttctattt ctggcaaatc catttcagca
    16801 atcagggtct ctggtgtgac aaggtatcct tctttcacga atacattgaa aggaacaccc
    16861 tttagttcaa cagtatctaa tcccatgtga acaagtactt ccagcccctc attagaaaga
    16921 ataccaatgg catgttttgt ttcaaagaca ctcgtcactt taccgtttac tggtgcatag
    16981 actttgccgt ttgacggttc gacagcataa ccgtcaccca tcattttttg tgagaaaact
    17041 gggtcattta cttcagttat ttccttgatg gtccctgcag cgactgcgaa taatgtttct
    17101 tttgttgcaa ctgccgtacc agttgctgaa acaggttcga aagactgtgg cgttgctgtt
    17161 gcgccatttg ctgtttgaag ttgtacatca gcagcatttt caggaacagc aggatcaatc
    17221 ttattcaaaa ttccctgacg tctaaagatg atcgtcaagc caaatggaat gatgacagca
    17281 atgatcattg cgatgatgaa aggtacccat gtttctgctt ggatagctag tattcctggc
    17341 aaaccgccta ctccgatcgc gttagctcga acacccatca agttagcaaa catgccagct
    17401 aatccagagc cgaccattgc agcgacgaaa ggatagacgt atttcaaatt gattccgaac
    17461 atagcaggtt cagttacgcc taagtaacaa gaaatcattg ctggaatcga gatttgttct
    17521 tcttttttat tcccacgatg taagaagatg atggctaaga ctgcagagcc ttgggcgata
    17581 tttgataaag caatcatcgg ccataaattc gttgatccga aatctgcaat cagctgcata
    17641 tcgatggcat tggtcatatg gtggagacca gtaatcacta aaggagcata caggaatccg
    17701 aatacggcac taaacaacca gctgatagaa gacgtcaatc cagtatttac gatatttgag
    17761 atccagcttc cgactgtcca gccgatagga cctaaaataa cgtgtgcagc taaaacagtt
    17821 ggtaacaatg aaaacaatgg aacaaaaatc atcgagatcg actgagggat atatttgcgg
    17881 aagaagattt ctagatacgc taacataaac cctgccagca tggctggaat aacttgcgct
    17941 tgataaccaa tcatctggac ttgagcaaaa ccaaaatccc aaaatggaat atctgctgcg
    18001 gctgtggaag ctacactata agcatttaat aattgcggag aaactaacgt aattcctaaa
    18061 acgattccta atatttgtgt agtacccatc ttttttgcaa tactccaagt gatacctact
    18121 ggaagaaaat ggaaaattgc ttcaccgggt aaccacaaaa aggcattgac accattccaa
    18181 aacttcgagt gttcaacgat tgttccacca agactttcaa actggatacc ttcaagtaca
    18241 tttctgaatc caaggatcaa tccaccgaca atgattgcag gaatcagtgg tgtaaagatt
    18301 tctgctaaaa cggcaatagc acgttgaacg ggatgaaggt tttgttttgc agctgctttt
    18361 ccttgttctt tggaaacccc ttcaactcca gatactgcaa cgaagtcgtt ataaaaagta
    18421 gaaacatcgt ttcctatgat gacttggaac tgtcctgcat tcgtgaacat cccttttacg
    18481 ctaggtatat cttcgatcgc tttttcgtcc gcttttcctg gatcattcag gacaaaacgc
    18541 atacgagtcg cacaatgact aactgcagca atattttctt ttccgccgat atcatgtaac
    18601 agcttctcgg catctgcttg atattttgcc atatcaataa acccctttct cttaacttgt
    18661 ctatgcaagt tgtttcttca tttactatac tttttttcgg agagaattgc aagcgtttta
    18721 ttaaaaagta cgagagaaaa tgatatcgtt ttaaaaagct taaaataagc ttttcttaaa
    18781 aataggaaca ttgaaataca aaaagagctg tagtatagtg aagaaatgat ggaagaacga
    18841 gtgagggaaa aaacatgaat aagttcaatg aaattttttt agatttggaa cagaaaattt
    18901 tagcgaaaga atatcctccg cattcgcttt tacccagtga gaaccaattg atcaaaattt
    18961 atggtgtttc acgagaaaca attagaaaag cgctgaatct tttgactacg gaaggttata
    19021 ttcaaaaaaa acaaggaaaa ggttcgatcg tactggatcg gaatcgcttc gactttccta
    19081 tttctggatt gactagctat aaagagctac aggagaccca acgtattcca agtgaaacga
    19141 tcatccatac gttagaagaa acagaagtga caaaagcatt gaacgagatc actggctggg
    19201 agatcggtgc gcctgtttgg catttgatac gcgaaagaaa aatcaatggc gaagtagtta
    19261 tattagacac cgattatcta ttaaaggaaa tcgttcctat tttaactcca gcacaagcac
    19321 aaggatcgat ctatgaatat tttgaaaatg agctttcttt gacgattgct tacgcgcaaa
    19381 aggagatcac agtagaagaa gtaacggaaa tcatgaaaac agcgatggat cttcacgatg
    19441 acacttatgc agtagtagtc agaagcctcg ttcatctgga agatacccgc tgtttcgagt
    19501 attctgagtc ggttcaccga ttagataaat tccgtttcgt tgagtttgcc cgacgccgaa
    19561 aagtgtaaat agttcaaaag aaagcaaatt gttggcatct gtccactttg gcgctatgat
    19621 aggatcacaa agggggcatt tttaatggat aaaccaataa gaccagaaaa agaagaactg
    19681 aaagaaaagt tatcacccat ggcttatgca gtcacacaag aaaatgcaac agaacgacca
    19741 tttagcggga aatatgatga tttttacgaa aaggggatct atgtagatat cgtcagcggt
    19801 gaaccgttat tcagctctgc agaaaaatat gatgcaggtt gtggctggcc atcattcagc
    19861 aaaccaataa cgaaaagaca agtgcgcgaa aaagccgatt tttctcatgg catgcatcga
    19921 atagaagttc gaagcaaaga agcagattct catctcggac atgtgtttac cgatggacca
    19981 gtagatcaag gtggtctacg ttactgcatc aattctgcct cactgaaatt cattccatat
    20041 gaaaagatgg atgaattagg ttatggggaa ttcaaatcac ttgtagaata atggaaatac
    20101 acaaagagcg gtacaatacg gggatttact cgatcgtccg ctcttttttt attcaagcga
    20161 ttgctatttg aaagaaacgc tccctagact cctttttttc acaaggtgaa atgtgctaca
    20221 ataaagggaa cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag
    20281 gtgtggttac ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc
    20341 agatcgcact aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt
    20401 atgtccatca ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag
    20461 aaaagcaact gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg
    20521 caatcatggc aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca
    20581 cttatttgac gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc
    20641 taaaaggaaa attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa
    20701 acacaaaaga cgctacagct ctatctgaag caatggaagc tctctcggct cttggttaca
    20761 gtgatcgaga aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg
    20821 atgagtatct acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga
    20881 cagatgaata cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc
    20941 ttcgaccaca gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga
    21001 tttacatcga agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc
    21061 ctcctggatt aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata
    21121 ttcgtacgac gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg
    21181 aactggaagc aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag
    21241 aagagatgct ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc
    21301 caaccgcaca tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac
    21361 gggcaggtat gctttctgca cctttacgag atcgatttgg catcatttct catatggaat
    21421 attatgacga acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag
    21481 agatcattga agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg
    21541 ctaatcgctt gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg
    21601 atcgtcctat agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt
    21661 atgttgacca gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt
    21721 taagtacgct ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac
    21781 cgtatctgat ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt
    21841 atgcctatga acattttggt tacgactatc atatatgatt tttgctttta gattggagga
    21901 aaatgaatga gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg
    21961 tgccaaacgc aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc
    22021 aatcgacaaa cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat
    22081 tgtgaggatg cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag
    22141 caagcattga aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg
    22201 ctttctgacc cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc
    22261 aagatttttg aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat
    22321 gctcgttttt tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat
    22381 gaagaaacac cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta
    22441 tattcttatc gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg
    22501 ttggaaaagc aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt
    22561 ttgggaaata tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta
    22621 gaaggaaggg aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga
    22681 aagacaccgc accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat
    22741 atggttgctc ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg
    22801 gatcaagaaa atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc
    22861 catttgtttt taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat
    22921 accaataact ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat
    22981 atttggattg atgaaaataa aataacaaac aatttgtaaa gttaacgctt tctttttaag
    23041 gcgatgttag agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa
    23101 gtaacacttt cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa
    23161 tgaagaagct tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa
    23221 atgacactga agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc
    23281 aaaaagctgc tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta
    23341 gcaagtatga aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga
    23401 tgatccgcct acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa
    23461 aaataacaat cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag
    23521 atagaaaagc acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc
    23581 tttaagctga tatcagtaaa acggaacgtt ttaggatgag tatcatagca tgacagcatt
    23641 cggttgatta gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct
    23701 agccggggag ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt
    23761 tggtatgctt atataatagt cattagttgt gaatattttg tgacgaaagt tttcgaaagt
    23821 tgttaagtt
//
LOCUS       DAYYGO010000033        23672 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000033 DAYYGO010000000
VERSION     DAYYGO010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.033"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(228..1115)
                     /locus_tag="ACHA5T_001097"
     CDS             complement(228..1115)
                     /locus_tag="ACHA5T_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8886686.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(1112..1981)
                     /locus_tag="ACHA5T_001098"
     CDS             complement(1112..1981)
                     /locus_tag="ACHA5T_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8886687.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(2118..3404)
                     /locus_tag="ACHA5T_001099"
     CDS             complement(2118..3404)
                     /locus_tag="ACHA5T_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8886688.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            3677..4255
                     /locus_tag="ACHA5T_001100"
     CDS             3677..4255
                     /locus_tag="ACHA5T_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8886689.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            4255..5184
                     /locus_tag="ACHA5T_001101"
     CDS             4255..5184
                     /locus_tag="ACHA5T_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8886690.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            5181..6917
                     /locus_tag="ACHA5T_001102"
     CDS             5181..6917
                     /locus_tag="ACHA5T_001102"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8886691.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(6980..7816)
                     /gene="pheA"
                     /locus_tag="ACHA5T_001103"
     CDS             complement(6980..7816)
                     /gene="pheA"
                     /locus_tag="ACHA5T_001103"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFM8886692.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(7813..8322)
                     /locus_tag="ACHA5T_001104"
     CDS             complement(7813..8322)
                     /locus_tag="ACHA5T_001104"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFM8886693.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(8315..9610)
                     /gene="aroA"
                     /locus_tag="ACHA5T_001105"
     CDS             complement(8315..9610)
                     /gene="aroA"
                     /locus_tag="ACHA5T_001105"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFM8886694.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(9623..10714)
                     /locus_tag="ACHA5T_001106"
     CDS             complement(9623..10714)
                     /locus_tag="ACHA5T_001106"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFM8886695.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(10731..11897)
                     /gene="aroC"
                     /locus_tag="ACHA5T_001107"
     CDS             complement(10731..11897)
                     /gene="aroC"
                     /locus_tag="ACHA5T_001107"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFM8886696.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(11899..12963)
                     /gene="aroB"
                     /locus_tag="ACHA5T_001108"
     CDS             complement(11899..12963)
                     /gene="aroB"
                     /locus_tag="ACHA5T_001108"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFM8886697.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(12996..14018)
                     /gene="aroF"
                     /locus_tag="ACHA5T_001109"
     CDS             complement(12996..14018)
                     /gene="aroF"
                     /locus_tag="ACHA5T_001109"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFM8886698.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(14677..15651)
                     /locus_tag="ACHA5T_001110"
     CDS             complement(14677..15651)
                     /locus_tag="ACHA5T_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFM8886699.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(15632..17515)
                     /locus_tag="ACHA5T_001111"
     CDS             complement(15632..17515)
                     /locus_tag="ACHA5T_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFM8886700.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(17533..19758)
                     /locus_tag="ACHA5T_001112"
     CDS             complement(17533..19758)
                     /locus_tag="ACHA5T_001112"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFM8886701.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            19873..20907
                     /locus_tag="ACHA5T_001113"
     CDS             19873..20907
                     /locus_tag="ACHA5T_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8886702.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(20974..21987)
                     /locus_tag="ACHA5T_001114"
     CDS             complement(20974..21987)
                     /locus_tag="ACHA5T_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8886703.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(22065..22253)
                     /locus_tag="ACHA5T_001115"
     CDS             complement(22065..22253)
                     /locus_tag="ACHA5T_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFM8886704.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            22800..>23672
                     /locus_tag="ACHA5T_001116"
     CDS             22800..>23672
                     /locus_tag="ACHA5T_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8886705.1"
                     /translation="MTYTHLTTDELVIIESYFKINQSVAKTAHCLNRSRQTIHKVYLF
                     FKKGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNN"
BASE COUNT         6807 a         5040 c         4286 g         7539 t
ORIGIN      
        1 tgacaatcag gacagtcatc ggcaatatag cagcaatcac ttctttaaaa ttttttagca
       61 tgtatcctcc tcctttactc tttaagttta ccttaaaatg aaagagtttt cgagcacaaa
      121 cttaaaacgt tatcatttaa tttttctcaa cataaaataa cacacgtccc aaaagtatct
      181 ttttacttgg aaaaatgact ttgaaggaac gcgcttattg tgttttttta ttttacagag
      241 ccgatcattc cttgaacgaa ttgcttttgc aaaacaaaaa atacgatggc agttggtaaa
      301 gtagcgatga ctaatccagc catgatcatt ccatagtctg gtgtatagga agcacccata
      361 gcagataaaa caagcggaac cgtccgttta tcaggagatt gcaaagctac taagggccat
      421 aaataattgt tccagctact catgaatgtg atgattgcag ccgcggcata ggtattttta
      481 gccgtcggca tatacactct aaaaaaaata cttagttctt ttagaccgtc taatcgagcg
      541 gcttctacta gatcttttgg aaaagattta acattttgtc gaaagaagaa gatcaaaaaa
      601 gcagtgctaa cagatggcag gatgacaacc aagaaactgt tgattcccaa aaccgttccg
      661 ttcaattgtg aaaacattcg atataaaggg atcatcaatg ccgcaaaagg aaccatcatc
      721 gatagcagta gaatattgaa aattaggtct tttcgtttac tttgataaat ctcaaatcca
      781 tatcctgcca ttgacgaaat cagcaatgca aacacagtgg tcaccaaagc gatcatcgct
      841 gaattccaaa gggaacgagt aaaattcagc tcattggaaa acaaattttg aaaattgatg
      901 atcaagttac tgccaatctt tatttttccc gatgtgatat ccacgggtgt attcgtcatt
      961 cccaaaacca tccagacaaa agggaaaatg gaaataaatg ataggatgct taatgctgta
     1021 tactttaata ctgacccaat tgtcgaacga agttctaatg cttgtttttt gacgttgggg
     1081 cgttcgattg tttctttttc taccatattt ttcatcgact ttctcctcct actttcgttt
     1141 ggattatcga caatatcaca ataaaggaaa caataacaaa ggaaactgct gccgcatatc
     1201 caaaattagg cgtgaattta taactcaaat tatagatgta ttgagatagg gtggtcgttg
     1261 catttgctgg tcccccgcca gtaatatttt gtacttcatc aaaaagctgc aatgtcccga
     1321 tcgtcgaagt aatggaagta aacaaaatga ttggttttaa atttgggatc gtcactttga
     1381 taaactgctg ccatttagaa gcaccgtcaa tagatgctgc ttcataaatc tccgaatcaa
     1441 tattttgcat gcctgaaagg aaaaaaatca tattgtatcc agtccaacgc caagtgatcg
     1501 aaagaatgat cagaactctc gcccaaattg gatgagtcaa ccagccaatc ggtgtgtcaa
     1561 tcataccaat gcccaacaac aagttgttta caaggccaga ttgagaaaat agacttttca
     1621 tgatcagaga ataactgacc atcgaagtga tacatggaag aaagatagcg gttcgaaaca
     1681 accctttata tttcaacttt ttatcattta aaaggttgga aataaaaagt gctagaaaca
     1741 acatgatcgg tacttgtaca agtaaataca ggaatgtatt aaaaaaagct tttttaaaag
     1801 ttgcatctgt caacatccgc tgataattac ctaacccgtt aaatgtcata ttgacagggg
     1861 gaccactttg aaaagacgtg atcaaagcca aaaccatcgg aataaaaacc atgattgata
     1921 tcaaaatgac tggcaagatt aaaaaagcat gcccgttttt gaataaatct ttctgcttca
     1981 ttcttttccc tccaattcta tcattgatta tctacatgaa aagacaagaa agacaatatc
     2041 tgtaaggcag acgtgatctt ttttgtcttt tcgtttgtct gatgaaaaag cgaagaaatt
     2101 cactttttta ccaccgatca attagccaat tgtgcttcta cttgtttttg tgtatctgct
     2161 agtaccttgt ctgtctcttc tccattgatg attcgatgaa gagcttcagc tacgactgct
     2221 tcaattgcat aggtctcatg tccgtaattc acttctggta tttcagatgt ccatgttgaa
     2281 aaatcgtcaa agatcttttg accactgtaa aattcagaag cttcttgata agcttcttgt
     2341 tcattcgcgc taagcatcgt agatactagt ccgatttctt tcgcaagagt gcccatcagt
     2401 tcttcattcg aagcaaacgt tgcttctagg aaatctttcg ctgaatcttc tccagctact
     2461 cctttgatca cataccagcc agcgccgcca aggttagaag cttgcgcttt ttgcagattt
     2521 tctggaagtg caggtattgg tgcaatcttc cattttccag attggttttc tgctccttgg
     2581 atcgttgaag aataccaagc gccagttggg ctagaagcta ctgctcctga ttgtatcgca
     2641 gttactccag cattccagtc agaggtttgt tcgactagat cttctttcaa aagagtggca
     2701 aacaattcaa gaccatactt taaagatgca ttgtcttgga tcgtgactgt ctccccatct
     2761 tcagatgtat accattctcc tgcctgttgc ataatcatcc ttactcgacc aaggtcagaa
     2821 ggattgactt cagcgatttt ctttcccgtc ttttctttga catctcgcgc tacttgaata
     2881 tactcgtccc acttcagatt ctccatatcc tcttctgtat aacctgcttc tgccatcaga
     2941 tccgtacgat agaagtttgc tgttacgccc gaatcgaaag gaacaccgta tattttatct
     3001 cctactttgt ttaccgcaaa tttatacggt gcaaaatgat cttcctttac aatagcagac
     3061 aaattagcga atgcatctgg ataagaagtc aaatagcctt gtatcctata atcttcaatc
     3121 aagacgatat tgggcaatcc ttctgtattc ccgctcgcta aagcggtgtt cagtttctga
     3181 acgatatcat cctgagacat cgtaacaact tctatctctg tttcttcgtt ctcataaact
     3241 ttttttgctt cattcactgc tttgatattg aatgtctcat cccacgccca gacagtgact
     3301 ttttcttctt tgtctgctgt actactagca cttccgcatc ctgttaagaa acctgatgct
     3361 gccgctaaac aaacgactcc agcaattaac tttctgatgt tcatttttat ccgctccttt
     3421 tccatccatt agttgtttac gtttgctaca ttcatcatac caaaatttga aaacggtttc
     3481 tttattattc agatacttca aaaatttctc ctaaaataat aaaaaaatac aaatcaacta
     3541 tgacaaaagg aataatctta gcatctagac caaagatcga aatggtgagg tttctttaaa
     3601 ataaaaatat ccttgcattt tctcctagaa ataagtattt ttaaattaca agactattct
     3661 taaggcgggt aaaaaaatgc caatacgcgt aattcttatc gatgatgaac agacgatttt
     3721 agatggaatg aaacagttga tcaattggtc tgagcacggg tttcaattaa tagggacttt
     3781 ttccagtccg ctgaaagcgc ttgatttttc tttggtacat gcaatcgata tcgtggtcac
     3841 agatgtaatg atgcctgaat taaatggaat cgaattatcg cggctattaa aacagcaaca
     3901 acctgaaaca cagatcctta tcctctctag ctatgatgaa tttgatatgg ttaaagattc
     3961 ttttaaagaa ggcgtcgctg attatctttt aaagcctcga ttgagtcctg atttttttct
     4021 aaattcctta actgccgttt cacaaaaaat caaaaaaaag cccaacaaaa gcccaatcac
     4081 caatcgttat gagcagatct gcgagaatct ttcacagcag attgctggac aaactcgtcc
     4141 attgcttgac cctcttcttt ttgaacacac cttgtttttc tgttatatac cgaaaaacgg
     4201 ccattcagtt caaaacaagg ctctaaacaa aaacagtcga tcacccattc attgatggat
     4261 agcgacgatt cgtttactat ttatcctgtt tctacctttt cggaagaatc cggctatgta
     4321 atcaatactg attccaaaga tacacttagg aagtttgttc aaaagtttcg tcaagaagtt
     4381 tttccaggtg atttttttgt tgtttctgat tcttttgcaa tccaagatat caatgaaata
     4441 ttccaagaga tcagaaattg caccaaagga caacgtttct atcataaggg aaagaacctt
     4501 gcttatcagc atgaacttct tccgttatta accaaccacg agcagcaagc aagtagttat
     4561 ttaacaaaaa tcgttcataa agactacgct ttagctgtca aagaacttga gaatttcttc
     4621 acgacgatat tggaacagcc tattgatcca gcttatttga aacatgaggc aatcaatcgt
     4681 ttctatgcat tactcaacgc tttggctgac gagcatgttg ggaatgaaga actttctttg
     4741 ctcaagatcg cccttccttc tttattagcc gatgctgagt atttcgaaga tttttctatt
     4801 cttgtcaaag aaacacttga gaaactcacc cacttttctg caaatgcaaa aaaagacaat
     4861 ttcgatatct tgactttgat ctacgagttc gtccaggaaa attatgagaa agaaatcaat
     4921 ttacaacttt tatccgaaaa atatcatttt tcatatagct atctctcagc catctttact
     4981 gaaaaatttg gtatcaactt ttcaaaatat ctaaaaaaag tccgtatcag taaagccaag
     5041 ctctttttga cggaaacacg gcttactttg acggaaatct gtgagaaagt cggctatacc
     5101 gaactgggtt atttctccag agtttttaaa gaagagacag gaatgacacc ttctcaatac
     5161 cgtaaaggaa cttttgtaaa atgagaaaaa aaatcaaaga gcatgaactc tttgccaaac
     5221 ttctgttcat tttgttcatc ggtctttttc tgcaagccat cgtcatttct ttgtttattt
     5281 atcatcgatc aagggatgcc tatatccagc tgttcaatca atcgaatgat gtggtactaa
     5341 aaaagattca atctgaattt gaaagtttga atgatacaat cgagaataca ttagccgctt
     5401 ttgattctaa tccagctgtc aaaagctatt tttccaatga tccagcccaa catatggaac
     5461 agcttcagca actaagaaca atccaaaaaa tgaatgactc tctttcaaaa atccatccaa
     5521 tgatcgatta cgatgtattg atctttggag aaaatggaag aacttttgtc ggaaacgaca
     5581 tgctcactgc cgtatctgcc gattcttttt ttcaatctgc gattgctcag agagtgaatg
     5641 aacgtgctgc tgatacacaa atgctttttg cacaccacgg attgactctt cgcgataaga
     5701 aagcacccag cgtcttcttt gtcaaaaaac tgaaaaatac attgaatcac gtttacggat
     5761 atgctgtttt atcgatctca tccaagcaac tagcgaatct ttttcaatct gtagtaaatc
     5821 ctgagatatc gaagatctcc ttcatgaacg aagaacagac gatcatcgca tcaaatgaag
     5881 aaaatacaat tgggagccga tcaatgattt tcaaaaaact aacagtaggt gaaacaacga
     5941 tttcaaatgg acagcagttg acccgtcttc ctctgtaccg ccaggattgt acattactca
     6001 gtcaagtcga tcttcattct ttatctaacc aaatgggcgt gatttttccg attattttgt
     6061 ataatatcgt gacacttgct tttgctggct cattggtatt tatgtatttg gatcgacaca
     6121 caaaatcgat ctaccgcttg attgattcgc taaaacggct agaggaagat ggcttaaaag
     6181 ctactgttcc tgtacaagga agccatgaag tccaattgct agggaatacg atcaatcaat
     6241 tgctaagtaa tgctcagcat cattatgaac agacgatcca agacgaaaag aagaaacgtt
     6301 cgcttgaaat ccaaactatg caagcacaga tacagcccca ttttatttat aatacgctga
     6361 ctagcttgaa atttttgatt tggcagaaaa aaacagatga agcagttcaa ggaatcgaca
     6421 gtttcattac tttactaaga agtacgattg ggaaaaaaga agaagtgatt ccagtcaagg
     6481 aagaaatcaa aagcgtacaa agttacatcg atcttttgag tatgcgatat ggcgaaagaa
     6541 tctcggctaa aataatgatt cctgatgaac tgcagtcatt atctatgccg aatatgatcc
     6601 ttcaaccaat tatcgaaaac gcctatcttc atgcttttca caataaaacg caaggattca
     6661 tcacggtgta cggcaaaaca aaaaatcagc ttcttttatt cgaagtgatt gataacggcg
     6721 atggcttcga tactgaaaat accaaacaaa aaaatgatca tttttcagga atcggcatca
     6781 gccatgtaga tgagcggata cgcctaatgt atggcgatgc atatggctta acgatccaat
     6841 cagtgattgg gacaggaacg actgtaacga tttgtctacc gcttcttcct aaaaatcaat
     6901 cagaatatca aaaataaaga atagccaagc gctgattttt ttattttaag ttaaataaat
     6961 caatcagtat tcttgacttt catctacgcg tctcttctac tgtcagcact ggataacagc
     7021 ccaatatttg actttctcct ccgagcatct tgatttcttg gagcgcattc tctactaatg
     7081 tcattggacg gtccaacaac aaatcgatca cgaaaaaata ttctcccaag ctagttttca
     7141 atggtcggga ttcaatctta cttaagttga tttcccgcca gccaaaagct gccagcattt
     7201 tatgaagcat ccctggccga ttagcaggta gtgtcaagat gacactcatt tttactggag
     7261 ctgactgctg gctggtcatc tttcggtcac cgacgatcca aaaacgtgtc tggttcaact
     7321 cattatcttg gatattctca gctaagatct ccaatccaac atgctgtgct gtttctaatg
     7381 aagcaatcgc tgctgcgtct tctttcggat gttctgctac atacattgct gcagcagttg
     7441 ttgattctgt tgctactaaa ggaacattag gataatgcgt ttctaaaaac tgttgtgatt
     7501 gtgctagtgc ttgtggatgt gaaaggattt ttgtaatctt tgttgctgga ttgcctaaaa
     7561 gctgctgctt gataggaaga acgatttcac ttttaacttc gacttctgga tgtttggaaa
     7621 gaagatcgat tgtgtgatga acagctcctt ccaatgaatt ctctacagga acgacagcca
     7681 gatcgacttg tttgcgaaac aaagcgcgca aacaaacagg gatggaagca tacgcagtca
     7741 attgttctgt aggaaatagc tgacaagccg cctgataagt gaatgaactt tctggcccta
     7801 gatagctgac gttcatatgc ttttgatcct ttctataatg gtatcgatga tcttttctgg
     7861 tgtttgattg gtcgtgttga ttcgatgtgt cgcactttct tcataccaag aaagtcgttg
     7921 tgccaataat gcttttattt cttctcttgt ttttccaaga gctaaaggtc gaatagacgt
     7981 ttcatcttgt tggatacgat caactagaca gtccgcttct gcctctaagt aaagaacaat
     8041 cggctgtgtt tttaaaaaac gccgattttc tgcactttgg acaatccctc caccagtcgc
     8101 gataattttg tcttcatttg acagctttct taaaatatct gactccactt ttcgaaagct
     8161 tttttcaccg taatagtgaa aatattcggc aatcgacaat tggatatgtt cctcaatctt
     8221 tgtatctaaa tctacatact ctttttgtaa acattgagct aaccctttac cgattgttgt
     8281 ttttccagcg cccatgaaac caatcagcaa aatgctagcc atgagcttct cctcctgaca
     8341 caagttctgc taaatcgtca aaaaatgccg gataagagat tgaaacagct tcagctcctt
     8401 ctagttcgca aggtccatca gctatcaaag cagctacttg cagcatcata ccgattcgat
     8461 gatcgcctct gctggacact tttgcgccat gcagaggtgt cggcccatgg atgatcagac
     8521 cgtctgctgt cgcttttatc tttgctccca ttttctgtaa ttcttctgcc accgcatcaa
     8581 ttctgttggt ttcttttact tttaattctt ctgcgtcttt gatgatcgtg attccttctg
     8641 cttgagtagc cactaaagct agaatgggca attcatcaat cagacgcgga atgatttctc
     8701 caccaatcgt tgctttcttc aaatgactcg tcttgacact taaatcagcc gattggttct
     8761 gttcattgtg attcgtctgg gtaatcgctg cgcccatctc ctccaacaca tctaagatac
     8821 cagttcttgt tggattgacc ccgacatttt ttaacaacag ttggctttcc ggaacaagaa
     8881 gtcctgctgc tagaaagaaa gcagctgatg aaatatcacc tggaacagtt atctgttgac
     8941 cagttaattt ttgcggtccg gtcactatga tcgttttatc ttccactgtg atccttccgc
     9001 caaattgacg aatcatttct tccgtatggt tacgggaacg ttctttttca acgattacgg
     9061 atgtgccttc tgcttgtaat gccgcaaaca gcaaagcaga tttgacttgc gcactggcta
     9121 caggcatatt gtatcggata ggtgataaag agcgggttcc tgtcacagtg attggtagat
     9181 actggtcatt ttcttctcct tgcaagtcag cgcccatctc gcgtaaaggt tccatcactc
     9241 gccccattgg acgtttagac aacgaagcat ccccgaacag agtagtcgta aaaggctgtc
     9301 cagccaaaat acccatcaat aaacgtatgg tcgtaccgga gtttcccatg tcaattgatt
     9361 cggttggttg gacaaatgag tggctaccgc gaccatgaat cttgatcacc tcttcctctt
     9421 cttcgatctc gacacctaat tgttggaagg catgtaaagt actcagacaa tcttctgcgc
     9481 gcaaaaaatt ttgtatagtc gtcgttcctt cagcaattgc tccaaacatg atgctgcgat
     9541 gggaaattga tttatctgct ggtatcctaa cagtccctct taatccatgt atttgttgta
     9601 ataattgcat ttatccagcc tcctatttga tccagcaatg ataactggta ttagacataa
     9661 tgattctttt tgcttgtaac aaatcctgtt ggtttttaaa actgatttgc agtattccta
     9721 aaatatcttc cctggtctct tggatcttca aattaatcaa agaaactccc gcttttccga
     9781 tcagtccagt tacttctgca ataacgccag gttcatctgg cacatcgata aataaatcaa
     9841 aaaaggcagg aatcgcgcct tgcttatgga caggcaactg gtctcgactt tcttttgccc
     9901 gattgaaaaa agaaaagatt tccgattggt tctcagtcat gatccattct gctatttgtg
     9961 tcatttcttg ttgccaatct gccaatcgtt ccaataatat ctgcttattg ctcaacaaaa
    10021 tatctgtcca catgacagga tctgaggaag cgatcctagt gatatctcga aagcctcctg
    10081 ctgccagttg tttggctcgc ggatactctt gattgaattg ttctgcctga ttgactaagc
    10141 cagctgccag aatatgtgga aaatgactta gcattcctgt tatacggtca tgttctcttg
    10201 ctgtaagagt tacatatttt gctttggttc cgcaaaatag ttcttgcagt ttttcaacat
    10261 cttttttcgt cttttccaaa ggtgtaaaga tgtaataggc attttcaaat agtttagcat
    10321 ctgctgcctg tatacctgat ttatgagaac cagccattgg atgtccaccg acaaaccgaa
    10381 gatttttctg ctcagcggat gtcacgactt gctgcttcgt actgctgaca tccgtaatga
    10441 tgacgtgatc agacaagttg attctttcaa ggacatcaag ataagctaag gttgtacgga
    10501 cgggaacggc taataagata acgtctgctt gttctgcacc tgtttcaaaa tcacttgcaa
    10561 cgtaatcaac gatctgctgt tctactgctt gctcacgggt ttttgactga ctatcccaac
    10621 caactactat cagttctgga ttggtctgac gaatacaaag tgccatagaa gcaccaatca
    10681 gtccaagccc cacgataaaa acatgttgtg ccatcttatt tgcccccttt ttagtaatgt
    10741 ttcaagtagt cacggtactc cttcactgca gctttcaact caacaagcga atcacttggg
    10801 aattttgaca atagctctgt cgctaccaca gtggctacaa cattttccgc aacgacgcta
    10861 gctgcaggaa ctgctgtact atccgaacgt tctacacttg ctttgtaagg ttcttttgta
    10921 tcgatatcaa cactttgaag tggcttgtac aatgtaggaa tgggtttcat gaccccgcga
    10981 atgacaagtg gttcgccatt ggtcattccg ccttcgaagc cgcctagatt attggtacgt
    11041 cggaaatagc ctctttcttc ttcccatagg atctcatcca tcacttctga gcctggtaat
    11101 gaaccagaag tgaatccgac cccaaattct acgcctttaa aggcattgat actaaccaca
    11161 gcttgtgcga ttttggcatc tagcttcgtg tcccattgta cgtagcttcc taatccagct
    11221 ggaacgcctc caacgagcac ttctacaatt cctccgatcg tatctccatt tttctttgtc
    11281 tgatcgatca aatcttttat tctttgctca actgctggat caacaactcg tacttctgac
    11341 gcttcagaaa tttctttgat ttcttgaatc gagatatttc caggtatttc tgcttggatt
    11401 ccaccaagac aaatcacgtg tcctgctacg atgatgtcaa gttcttccag aattttcttg
    11461 gcaattgcgc cgattgctac acgcatggtc gtctctcttg cagaggaccg ttcaagcaca
    11521 ttccgaagat ctcgatgatg gtatttcata ccgcctacca aatccgcgtg tcctggacgc
    11581 ggacgtgcaa ctcgccgcag tgattggtct ttttcaggca cttctgctac agacatgaca
    11641 gttgtccaat ttttccaatc tttattttct accactaaag tgacaggtga accaattgtt
    11701 ttgccgtgac ggatgccaga cgtgatccga acacgatctt tttcgatctt catccttcct
    11761 cctctgccgt aaccagcttg tctgcgagcg agttcgtgat tgatttcttc ttctgacaga
    11821 ggtaatcctg ctggtagtcc ttcaataatg gctgttaatt ctggtccatg ggattctccc
    11881 gcagtcatat aacgcatctt acagtcctcc taagtaatct ttgaatgtct ctgtgggaac
    11941 aggaacgatt tttgcttgcc caatcttttc tagcaagatg atattcaatt ggcttcctct
    12001 tgctttctta tcatgggtta tcgcgtggaa taattcatct ggatgactta gatcgtacgt
    12061 aatcggcaga tggaattttt gaagtatcct ttctaaagca gctgtcgatc cagttggaga
    12121 caagccgatt ttttctgcat gagaagtgat ggctaccatt ccaatagcta ctccttctcc
    12181 atgactgact accccatatc ctgctgtatt ttcaattgca tggccaattg tatgaccaaa
    12241 atttaaaata agtcgatttc cttggtcaaa gacatcttct tccactactt ttcgtttgat
    12301 ttccaacgta gcagtaatga ttggaacagc atgttccaga agatcttttt cgtctttcag
    12361 ctcgttcagc tgtttccaaa gcgttgcatc tgctatagct gctgatttga tgatttcagc
    12421 gattccttct cgtaaacgac gtaccggcaa agtttttaga acctcaggat caatcaatac
    12481 accagctggc tggctaaatg ttccgacaag gtttttcgct ttgtttgtat tgactgctgt
    12541 cttcccgcca atactagaat ctacttgtgc aagtagcgta gtcggaattt gaacgaaatg
    12601 gatcccacgc atgaacgtcg aggccacgaa tgctgctaga tcacccacca ttcctccacc
    12661 aagtgcaagt atcccatcgc tgcgggtaaa atcattatct gctaaaaaat cgtataactc
    12721 agctgcttgt tctaaagatt tactggtctc acccgccggt acgattgaaa ggactggttc
    12781 aaaattagct tgtctcaatt ggaacatgat tttttctgca tatagtggag caacgtttgt
    12841 atcagtgatc acagctactt tttgaggtct ccaaatcgtt tgaagccatg atccagcctg
    12901 ttctagtgcg cctttttcaa tatgaatatc gtatttttgg ttatcaacat gtacagtaag
    12961 cattcgtttc acttctttct tttaaaaagc tggaattaat cgagtgtctg aatcttttcc
    13021 attgcttctt tcatgatcgc aacttctttc atcatgcgtg agtaagtctt ttcattcaac
    13081 gattggggac cgtctgacca agcatttgca ggatcaggat ggatctctac gatcatgcca
    13141 tctgctccgc ttgccacacc cgcacgagcc attggtggta caagttccca aattcctgta
    13201 ccatgacttg gatcaacaat cacagggaaa tgagttaatt ttttcatcaa tgggacagca
    13261 cttaaatcaa acgtattacg tgtcgctgtt tcgtaagtcc gaatcccacg ttcgataaaa
    13321 atgacaggag atttgtcttc gactgcaatg tattctgcag cattaagcca ttcattgatc
    13381 gtaccggaaa ttccacgttt caatccaaca ggtttacctg ttttaccgac agcagatagc
    13441 agtttaaaat tttgcatatt acgtgcgcca atctgtagga tatcgctgta ctcagccacc
    13501 atatcgatat gcccttcatc catcacttca gtaatcactt tcattccaaa ttcatcagcc
    13561 gcttgacgga tgtatttcaa tccttcttct tctaaacctt ggaaagcata tggagaagta
    13621 cgtggtttga aggcaccacc tcgcagaatt tttgcgcccc cggctttggc cattcgcgca
    13681 cattctctga tttggtctaa tccttcgata gaacaaggac cagccatcgt cacgaaactg
    13741 ccgtcaccaa tttttactcc gtctacatcc acgactgtat tgccaggatg gaattctcga
    13801 gaagtcaatt tgtatgtgtt taaaatacgc acagcatttt ctactccttc atagcgtgta
    13861 aatgctcctt cttgtaaact tcgcgtatca ccttttagtc caatcactac tcgttctgtt
    13921 ccttgatcga ttgagacttt gaatccttct ttttcgatat gatcgacgac tttttttatt
    13981 tgttcttccg ttgcttcttt tttcataatt actatcattg tttttcccgc tttcttttgt
    14041 ttacttatct atcttggctg attttctcta ctttttcaag gtggttttac attaattcct
    14101 actaaatgac cgtgtcctgt tgttctcatt ttcgctcctt ctttcctact tatgacccag
    14161 ataatcaaca aaaaaagatg tccctgtagg aaattgcctc tacagtgaac atcttcaacg
    14221 agccttaaaa agcgctgcaa aaatataaaa tagccactat agatgaattt ctctacgtgg
    14281 ctgtaagtcg tgtttaaaaa tctttagcca tcatagatac aaacctggtt tgtttgtacc
    14341 cacgaagtca tgaatacaaa cctatctaaa atagctaaag taatacgtat tcagttttgt
    14401 aagttttcta gatttcataa aaaatcgctc caaactgatg taagaattgt acctagtata
    14461 gacataaaaa aatcgactgt caatcatttt tatgaaactt tttttaattt tctgatagtt
    14521 aactttgttt ttcagaaaaa taacaattgg ttttattgaa gaaaatagtc atgaacaaaa
    14581 gtatctgaga acttttttac tctcttgttc atgacatata gaataaagca gctttgtctt
    14641 gtaaactccg tttttcaaat aataatctaa agattgctac tgaataataa agccgatttc
    14701 atggtcctct tcgctgctga ttcggtaagg tgactcaaca tccgctcccc agctattgat
    14761 acccccgact cctcttactt ttgcaaaaat cgtaagaacc gttcgtcttt ctggcggtag
    14821 ttcttctata tgtgttgcct gttccagttc ttctgctttg aatggtaaag cggaaaaagc
    14881 aaatggcgta tcctgctgtt caaatcgcaa cgaaaaatct tcttgatctt tatccgtatt
    14941 atccaacgtt tgatttcttg ttagttctac ccatttcgtt tccatatgca tgccgcattc
    15001 ttggggaaca aggtatgggg aaacggggag tcctgccact ttataaatac cttcgacacc
    15061 accagccatc cgatccggat atgtttcccc tgataggcct tcatactcaa aaaattttgc
    15121 tttcgttggc ataatgaacc ttaaacccaa tgcgggtaat tccggtaatc ctttttgtcc
    15181 ataataatgc atattgacaa gcattttacc ctctccagcg atttggtagg aaattgtcac
    15241 ttgggttttt ggtactgtgt tgatttccag tgtaaaaatg atttcaaccg tttcagcgta
    15301 ttcattactt gaatattgat tatttactgg agcaatcggc aaatcgatct ttttattgtc
    15361 caccagtact tctatttctt tgacttttgg atacaatccg gctcctaacc acataccgga
    15421 tttaaatcca aatccgctac ctctgtcatt atcggtcgtt gctcgccaaa aaactggaag
    15481 tgtttcccgg tatagccatt cttttccgtt tttattgagc gattccagtc cgcctcttgt
    15541 ataagaaaaa atataatgcg tattccccga tttgacacct aatgtacagt cgccgaagac
    15601 gattgcaact ttttccgtat ccatatgact tttatttact gtatttttca taataatatc
    15661 ttacctcctg catggcgggt ttttcttgtc catctgcaaa aacgatccca ttccctgaga
    15721 attcatagtc acttggttta tcgtcaaagt caccgccata gcgcatcact tctctgcctg
    15781 tgattggatc agtcacttgg atcgcctgat ctttaaaatc ccatatatag cctccttggt
    15841 acatttcata acgatcaagc aacgtcatgt aagaatccat tccacctaac gaattcccca
    15901 tgtcatgcat atattcacat aagatgaacg gtttttccgc tttattttca aggtagtcaa
    15961 tgatctcttc tggtggcgca tacatgcagc tttttacatc tgaaatcgaa tcttcgaatg
    16021 ctggatttgg aaaattccct tcataatgga caagtcgatc ggggtctact tttttgaagt
    16081 aatcgttcat ttgtcgaatg ttttcttccg cataagattc atttccaaga gaccaaaaaa
    16141 gaatacttgg atggtttttt aatgtttcga aattcgtttt tgctcgatca agaacagccg
    16201 cctgccactg aggtactgca cctggtacgt tgtaagaagg ctcaaatgcg cccatttttt
    16261 gccatgagcc atgggattcg aggttcgttt ccgccatcac atggattcct gcttggtcgc
    16321 ataaataata ccaagtgatc tggttggggt aatgagatgt ccgtacgccg ttgatctttg
    16381 cattttttat gatagccaaa tctttttcca tatcttctat agtgatggcg cgtccttttt
    16441 tcggatgcca ctcatgtcga tttaccccgt tgataatcat ccgttttcca ttgaagtaga
    16501 tgattttatc tttgatttcg attttacgaa aaccgaagga atagtcaata tactccgtta
    16561 ctcttttttc ttcgtctatc gtctccaatt caagcttata taaattgggc tgaaaatgcc
    16621 cccacagtgc cacattttca aatccatcga tgcgaatcgt catttgctct tttaacggat
    16681 attgctgttg atagatttcc ttcccatcag gatctagtat cgctacattc agctgactgt
    16741 ttttggctac ttgctcaaaa gacaaatcta cttcaagttt gccattttga catttgtcat
    16801 ctaagcgagg tttgatccaa agatcttcta aatggttttg tcctcgagca actaagaaga
    16861 catctctgaa gatcccaaaa aatcggaaaa agtcctgatc ttccaagaat gcagctgtac
    16921 ttcttttgta aacctccaca gctagaatat tttcttcttc caaataaggt gttaaatcaa
    16981 attcagacgg agtgaacgaa tcttcggcat acccgataaa atgaccattc aaccaaataa
    17041 acattgcttg ttctacacca gcaaaatgaa tcgagatttg ttttcccttt agaccttctt
    17101 ctaatcgaaa agtcgtcaaa tacgaaccaa ccggattgtc tttagcagct gaaaagatcc
    17161 cttccgcctc tttctcaggg cttgtcgtga aagggggacg gcgaaataca tgtccctccc
    17221 acggatacat cgtattgatg taatggatct tgtcataatt tgctaattca atatgactag
    17281 gaacagtgat ttcatcaaaa gaacaggcat caaaatcttt tttataaaat gtttttggtc
    17341 gtaccgaagg atttggcgaa taacaaaaac gccatgatcc atttaaagac tgtttaaggg
    17401 aggatgtggt ttctacttct tcccttgttg cataacaaac atgatcgcta tgtgcatttg
    17461 attggttcac tctaaaaact tgtggatcat ctaaccattt tatatttgct ttcatacttt
    17521 tttccttctc tctcataact ttttgaaatg aagcagttct gtttggaaat caccttgaaa
    17581 atcggggaag taaagtccaa ctgtcatcaa ttcggccccc gaaaatatcc gcttcgaggt
    17641 ctgttcctga taaagtgcct catcatctaa ccccatcaga tatacttcat ggaacgctgg
    17701 ttgagctgaa gctagtatcc tccctaaaaa cacaatagct tcatcttgtt taggagacac
    17761 aaacatccat gcggcaatat tttcttcaaa aggactcgcc aaacgataat aatggccata
    17821 ctggatcacc ggacgaatcg tttgatagga tataatttgg tcttttactt gctttttttc
    17881 ttcaggagac agctttgtca aatccagttc ataaccaaaa acagcgctca tagctgtttc
    17941 tcctcttgtt ttcaacgaag tagatctgtc ggtctgatga tttggtacag ctgatacatg
    18001 agctgtgatg gacgaaatcg gataagcaag gcttgttcca tattggattt tcatacgctc
    18061 cactgcatcc gtgttatcac ttgtccatga ctgcggcata taataaataa acccggcatc
    18121 aaaccgtcca cctccaccag aacagccttc ccataagatt tccggataat cagttgtcaa
    18181 atgttcaagt agttcataaa gcccgagcat ataacgatgc gctacttctc cttgtctctg
    18241 tgggtcaagg gtaatcgaat agacatctga aagcgaacgg ttcatatccc atttgatgta
    18301 gtcaagagga atcgtatcta aaatctttct catttgtttc tcaatcgtct gccgaacatc
    18361 taaccttgta aaatccagta catgttgtga tctggaagca gaaggcattc tacctggctc
    18421 ctgcataaga aaatcaggat gtgttcgata cagttcagaa tcaaccgaga tcatttcagg
    18481 ttcaaaccat aaaccaaatt tcaatccttt gctatgagca taatctgcta tttcacgtaa
    18541 tccatttgtc aacttgcctt catactcgaa ccagtctcct aatgaagaat tgtccgcatc
    18601 tctttttcca aaccagccat catctaatac gaacatttcg atgcctagct cctttgcttc
    18661 atcaatgatc gccttgattt tttcactatt gaagtcaaag taagtggctt cccagttatt
    18721 gaccagaatc ggccgttcag caaattgatg acgacctctt gcgattcttt cacgtagcaa
    18781 ctcatggtgg atctgactca ttttgttcaa tccctgactt gagtaagaaa ggatcatttc
    18841 aggtgtttga aaagactggc ctgcattcaa ttcccacgaa aaacgatggc tgttgatccc
    18901 accgacgact ctcaattgat tgatctgatc tttttcaagt tcgaaagaat ggttgccgga
    18961 ataaacaaat tgaagtccga tcgcttctcc agtattttct gtcgtatgat gatgaactaa
    19021 agcaataaag ttattcatat ggtgactgct cgttcctcga cggctttcaa atacttttgt
    19081 tccagataaa acagattgcc gtgaaatctg tctttcattg acatgtgctc ctggcaaagc
    19141 aatgacttca tcaaatcgcc tagtatgggc aaagtctagc tggaaactag ccgctttttc
    19201 tagtttgatt gtttctccgg tttcatttct tatctttaca gatcgtgtga tcactgctct
    19261 atcagtaaat atggtataga acaaatcgaa ataaatcgct gtttctctat ccttaagtga
    19321 aatcaccaac gtttgtgcct ctgacttgtc catcacataa gtttgaggca agccttcgat
    19381 ctctggcttc ccatctgtaa tataatggct gtcatatctc agatccagta agtctgatcc
    19441 attcttgcgc tgaatgatcg atgcgggcgt tcgaaagtcc atgctgccat gagaagagta
    19501 ttcttgcggt agtgtatctt tcgacagaga ccggtcagga tttaagggaa catttcctga
    19561 aaatccacga tctcttcggg gataattttt attgtctttg tactgcttga ctggtttgcc
    19621 aaaataaata tgcgccaata cattgctttc ttctatcttt atcagataag aaagaaatgt
    19681 atttcgtaaa tggaagactc ggctcttttc atcaaataag atgaaatttt tatctgtttt
    19741 tctatgcgta atatccatca tttcactccc aacaaaacgg aaaccgttta ctttttttta
    19801 taattcctgt atactgttta ctaaacaatt ttgtcaataa aaatttttta tttttttgag
    19861 gagtgtgtaa aaatggttgg aattagagat gtcgcacgaa aggcaaatgt ctctcctgct
    19921 acagtttccc gtgtgttgaa taacgatcag acgatccatg tggctcagac tactcggcaa
    19981 cggatagtag aggcagctgc tgcgttggat tatgacatca gtactcgtaa atatatcaaa
    20041 aagagaatgc cttcgattgg tgtgatctct acgatcggca aagcgagcga agaacaggat
    20101 atctattaca aacatttgcg agtagggatg gaagaagaag ctcgacgtct gcatttaggg
    20161 atgaatcgaa tctataatct atctgataat cctaaacaat gggaagatct agatcagtta
    20221 ggtgcggtga tcgttgttgg gactgttacg aaagaatcga ttaccgcctt gttaaaacaa
    20281 aacatgcaaa tagtcgtcgt ggataaccct gacatccagc aagaggtcga tatggtgtat
    20341 gcagatcttg aacggatgac taaaaccgta ctggaattgt tcttagaaaa tggtcataaa
    20401 agaatagcct atatcggggg atataaagtc gatatggatg aattcggtgg gaagcagatt
    20461 tctataaatg aaaagaggat gcgtgcctat cagcatttta tgatcgatca tcacttagaa
    20521 aaatatatgt cttatcatct aggtggctgg acagaaaaac aaggtgaggt acttgcgact
    20581 aatctattag aaaatgaatc ccctcttcct actgccattc ttgtcgggag cgatccaatg
    20641 gcaattggtg tctatcgggc attgaaacaa aaaaatatcc aaattggaga agagattgtt
    20701 gtagcaagct ttgataatat cgagacaatc gcaaacctct cccctggctt gaccagtgtt
    20761 caaatcaatg cgaaatcaat tgggcaagca gctgttcgaa tggcattgga gcgaattgac
    20821 ggtatacggg acgaaccaat cattttgact tatcctgcac gattgattgt tagggaaagt
    20881 tttatcccaa gaacaaaaag gtattgaaag agaggttgtg aaaagccgct tcgtttcttc
    20941 atactcaaaa cgaaacggct ttttcacaac ctcttactta tacttattca caaaatccaa
    21001 tgtctgctgg taacaatttt gcgattcttt tagcgtgtaa tcaaattggt attcatgggt
    21061 aatctctttt tttgtatctg tataaaacaa ggattgcacg ggtaccttta attctgtcaa
    21121 ccgattttct aatgcttgac cttgctcctg gaatgagtat gcatttccgt cagttatata
    21181 cgttggcgga aattccttag aaacatggtc agccacacta gcttctttca attgctcact
    21241 cgttttccaa tcttttgttc caatcaacga ccacgcaacg gttttgacga aaaacttcat
    21301 aaatcgatca tccgaatgct gctgtgctgt tagcttcaaa tcaacaggtc cgcaataaga
    21361 aatggttcct ttcagactat cttttggaag tagctgtttg attttcattt gtttagcata
    21421 cgcatcattc gtttgtatag ctgcatattg caatgcgatt tgtgaacctg cactatctcc
    21481 accaaaaaat aattgttcca tgtctagcaa tttgtcgtct ttttcttcag ctaataaatc
    21541 tttgatcagt tcattcactt gaagaacttg attagggtat tctgaatcag gcgctaattc
    21601 ataattcatc gcaaccactg cgatgttggc gtctgaaact aattttgtcg caaattcttt
    21661 gacacccgat ttatctccgc cgacgaatcc tccaccatgt acccaaaata gaacaggaac
    21721 aggccccttg ctgttttttg gatagtagat gtcataagta ttctctttat acttggactc
    21781 atacgtcaaa tctgtttttt tcgccacgtt tttttctgct tgatcgaacg tttttttatc
    21841 agtgatttga acagtatttg aaaacatatg gttgatcaag aatgctccag gtctggggga
    21901 aagctggaag gcaagaaaga ttgcaaacgc gatcccaaga atgattccga agaaccatcc
    21961 gataaattta agaattttct ttttcataag gctccctcag tagttagtat taagcattct
    22021 gattatacca tagtgttatt tcctatcaag ctctcctaga tgatttaatg attttcccca
    22081 aacttcttct gcaatgcttt tgacaaaaga aattttttcc attgttcttc aaacaaaaca
    22141 ttttcttctt ctgttgaagc aaatccacat tgaggactta agcacaaacg atccaaaggc
    22201 agatatcgtg tcgcttcgta gatccggtca atcacttgct ggtgctcttc catttcgcca
    22261 tttttcgtta cagtcaaaat ctaagacaac taatttatct tcagagactt tctctaaaag
    22321 aacaaaactc ccagcacgtt cactatcgta ttctaaataa taggcacgga cattttcttg
    22381 agtaaaataa tgggtctgct acgtcgtcat cccccctgat gccgccaaaa tcgaataata
    22441 atttccgcgt caaacatgta tatttagtgt tgaatcattt gagaatatga aaataaaaat
    22501 ctttttgatc aatcagttaa tcaaattgaa ataacgaaaa atctgagaca gaatgacgtt
    22561 cattctgacc cagatttgat tttgcacaac tcaacagtcc ttattggcaa atcgccaaaa
    22621 taatgtagac taactaaaaa ggctctataa tttatgggac tgatgaatca gaattgattc
    22681 ttcagcccca tttttatttt aggtatcgcc gattgtaaaa ttaagctaga caactaaaaa
    22741 agtcatttgt gctacactca aaatagttcc gaccaaagaa ttataaggag tgagtacaaa
    22801 tgacctatac ccatcttaca acggatgaac ttgtaataat agagtcttat ttcaaaatta
    22861 atcaatctgt tgctaaaact gcccattgct tgaatcgttc aagacaaacg atccataaag
    22921 tatatctgtt cttcaagaaa ggaaaatcag ccttagaata ttatcaacag tataagaaaa
    22981 acaaatcaaa ctgtggtaga cgtccgcttg ttttacccga ggaacaatca gaatatattc
    23041 aaagaaaggt tgttcaagga tggacacccg atgtgattgt tggtcgtgca gcgtttccta
    23101 ttagttgttc tgctcgtacc atttatcgta tgttcaaaaa ggggctattt gattcttctg
    23161 acttaccgat gaaaggcaag cgtaaaccga atggacatca agaaagacgt ggaaaacaaa
    23221 ctttccgccg ctctattcat gaacgtgaaa aggattatag ccaattctca aatgagtttg
    23281 gtcaccttga aggtgacact atcgtaggtc tgaaacataa aagtgctgta attaccttag
    23341 ttgaacgatt atcaaaagtt atcatcacat tgaaaccgtg tggtagacaa gcgattgata
    23401 ttgaaaaaaa attaaatcaa tggtttgaat ctgtaccgaa aaacctattc aaatccatca
    23461 cttttgattg tggaaaggaa ttttcaaatt ggaaacagat cagtaatgtc aatgatattg
    23521 ccatttattt cgctgatcca ggaacgccgt ctcaaagagg cctaaacgag aattctaacg
    23581 gattgttacg tagagatggt ttattgaaat ctatggattt caattcagta gatgaatttt
    23641 ttattcaatc tgtcgcatct aaacgaaata at
//
LOCUS       DAYYGO010000034        23654 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000034 DAYYGO010000000
VERSION     DAYYGO010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23654
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.034"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(27..602)
                     /locus_tag="ACHA5T_001117"
     CDS             complement(27..602)
                     /locus_tag="ACHA5T_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HFM8886706.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(607..1581)
                     /locus_tag="ACHA5T_001118"
     CDS             complement(607..1581)
                     /locus_tag="ACHA5T_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HFM8886707.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIASALKVNPNIGKHMERFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEEAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(1605..1649)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1864..2832
                     /locus_tag="ACHA5T_001119"
     CDS             1864..2832
                     /locus_tag="ACHA5T_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8886708.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            2929..3222
                     /locus_tag="ACHA5T_001120"
     CDS             2929..3222
                     /locus_tag="ACHA5T_001120"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8886709.1"
                     /translation="MRSAAYGNQRRKNRLALFNDEQQEIGEMTWSDAGPDIMIVDHTF
                     VDPAYRGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            3340..3576
                     /gene="secG"
                     /locus_tag="ACHA5T_001121"
     CDS             3340..3576
                     /gene="secG"
                     /locus_tag="ACHA5T_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFM8886710.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3679..4434
                     /locus_tag="ACHA5T_001122"
     CDS             3679..4434
                     /locus_tag="ACHA5T_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8886711.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            4437..6794
                     /gene="rnr"
                     /locus_tag="ACHA5T_001123"
     CDS             4437..6794
                     /gene="rnr"
                     /locus_tag="ACHA5T_001123"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFM8886712.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            6816..7280
                     /gene="smpB"
                     /locus_tag="ACHA5T_001124"
     CDS             6816..7280
                     /gene="smpB"
                     /locus_tag="ACHA5T_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFM8886713.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            7582..8301
                     /gene="atpB"
                     /locus_tag="ACHA5T_001125"
     CDS             7582..8301
                     /gene="atpB"
                     /locus_tag="ACHA5T_001125"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFM8886714.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            8354..8569
                     /gene="atpE"
                     /locus_tag="ACHA5T_001126"
     CDS             8354..8569
                     /gene="atpE"
                     /locus_tag="ACHA5T_001126"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFM8886715.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            8660..9184
                     /gene="atpF"
                     /locus_tag="ACHA5T_001127"
     CDS             8660..9184
                     /gene="atpF"
                     /locus_tag="ACHA5T_001127"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFM8886716.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            9171..9713
                     /locus_tag="ACHA5T_001128"
     CDS             9171..9713
                     /locus_tag="ACHA5T_001128"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFM8886717.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9736..11292
                     /gene="atpA"
                     /locus_tag="ACHA5T_001129"
     CDS             9736..11292
                     /gene="atpA"
                     /locus_tag="ACHA5T_001129"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFM8886718.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            11306..12208
                     /locus_tag="ACHA5T_001130"
     CDS             11306..12208
                     /locus_tag="ACHA5T_001130"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFM8886719.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            12231..13637
                     /gene="atpD"
                     /locus_tag="ACHA5T_001131"
     CDS             12231..13637
                     /gene="atpD"
                     /locus_tag="ACHA5T_001131"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFM8886720.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            13651..14073
                     /locus_tag="ACHA5T_001132"
     CDS             13651..14073
                     /locus_tag="ACHA5T_001132"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFM8886721.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            14542..14772
                     /locus_tag="ACHA5T_001133"
     CDS             14542..14772
                     /locus_tag="ACHA5T_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFM8886722.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            14869..16170
                     /gene="murA"
                     /locus_tag="ACHA5T_001134"
     CDS             14869..16170
                     /gene="murA"
                     /locus_tag="ACHA5T_001134"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFM8886723.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            16183..16359
                     /locus_tag="ACHA5T_001135"
     CDS             16183..16359
                     /locus_tag="ACHA5T_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFM8886724.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            16494..16790
                     /gene="yidD"
                     /locus_tag="ACHA5T_001136"
     CDS             16494..16790
                     /gene="yidD"
                     /locus_tag="ACHA5T_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFM8886725.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            16854..17321
                     /locus_tag="ACHA5T_001137"
     CDS             16854..17321
                     /locus_tag="ACHA5T_001137"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8886726.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            17406..18290
                     /locus_tag="ACHA5T_001138"
     CDS             17406..18290
                     /locus_tag="ACHA5T_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8886727.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLIGAAANYLAVRQGEN"
     gene            18404..18640
                     /locus_tag="ACHA5T_001139"
     CDS             18404..18640
                     /locus_tag="ACHA5T_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFM8886728.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            18728..18808
                     /locus_tag="ACHA5T_001140"
     tRNA            18728..18808
                     /locus_tag="ACHA5T_001140"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18762..18764,aa:Tyr,seq:gta)
     gene            18815..18886
                     /locus_tag="ACHA5T_001141"
     tRNA            18815..18886
                     /locus_tag="ACHA5T_001141"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18847..18849,aa:Gln,seq:ttg)
     gene            19012..19182
                     /locus_tag="ACHA5T_001142"
     CDS             19012..19182
                     /locus_tag="ACHA5T_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886729.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            19402..20109
                     /locus_tag="ACHA5T_001143"
     CDS             19402..20109
                     /locus_tag="ACHA5T_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8886730.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRRDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(20168..20812)
                     /locus_tag="ACHA5T_001144"
     CDS             complement(20168..20812)
                     /locus_tag="ACHA5T_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFM8886731.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKNFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSANHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            20939..21226
                     /locus_tag="ACHA5T_001145"
     CDS             20939..21226
                     /locus_tag="ACHA5T_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886732.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            21334..21654
                     /locus_tag="ACHA5T_001146"
     CDS             21334..21654
                     /locus_tag="ACHA5T_001146"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8886733.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIA
                     LIGE"
     gene            21735..23048
                     /locus_tag="ACHA5T_001147"
                     /pseudo
     CDS             21735..23048
                     /locus_tag="ACHA5T_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
     gene            23045..23464
                     /locus_tag="ACHA5T_001148"
     CDS             23045..23464
                     /locus_tag="ACHA5T_001148"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886734.1"
                     /translation="MRCSCCGKDIIQNLTFSEIIGVSKSRLPQTCSVCRAQFHLLEAK
                     GCAGCQKPIHLKYEETKKKANTSFVYCNDCQEWKKKYPAYDFQHRAFFTYDEKMQAWI
                     NRYKFMGDIRLAGTFAYKWTQIKKRLPGIYLLSNTFI"
BASE COUNT         7820 a         4125 c         5092 g         6617 t
ORIGIN      
        1 tattttgcta atccagctct gtttattcag cctatttttc tttttgaata tatcaagcgt
       61 tgtggcaaca tagtgatgac taatgaaaca acgacgatag acaacaaacg atcaaggtag
      121 tctgtacctg cctgtaccaa gatcacactt gttgtctgac ttaaacctaa cccatgtaat
      181 agctgaacga tcatactgga tcctgaggag gtgatcccgc caaataactg aacggtaatg
      241 atcgaagaaa cgatcgttcc aggtaccgac aaaatcaaag cgagtaataa cagtttcaag
      301 ctgtttttat ctttgccgag atatcttggc aaaatcaatc cagcaagcaa gcctgtgaga
      361 atttggattg gcgaataata cagagaaaaa atatctgtag tcactccact taaaataccg
      421 ctggacaatc ctgtcagcat tccaggtaca ggcccaagca atgcaccagc aaaaatcgtt
      481 ccgatagaat cgagatagat tggcaaacga agccataaag caatcgttcc tccaatataa
      541 ttgatcccaa tacacaaagc catcactgta agtacgcgag ttgccatttt gtttttattc
      601 attttgttag cccatcttta acctttccaa atctttcata atcgtttctt cttgtgcatt
      661 taaaagaacg gttaaaaact ttcggaaaaa aagacgagta tctacttttg tcataatttt
      721 actgttagca ggtttctgcc aaaattcata acgatcaacc aatgtttgtc cacgacttat
      781 tccttgagtt tccacagcag tgaagctttt gaaaccttca cagatggcct cctcgataaa
      841 ataagcaaca gctaacggat cattgatgac acatcctaaa attctttctt gtttccagtg
      901 gaagtcaaaa taaaaacgag tgatcgcttt tagatactct ccctcttcag gattcatttg
      961 acagcagtat tcgaggatag tcggtgtcaa gacgatttct cttgtcacat ctaacccaac
     1021 catttctatc gtttgtttga gattctcgaa tacataagaa gctgcttcag gatcacacca
     1081 gtaattgtat tcagcaactg gcgaacagtt accatggctt ttgtaagttc cacccatcga
     1141 gacaaaacgt tccatatgtt tcccaatatt tgggttgact ttcaaagcag aagcgatatt
     1201 ggtcaaagga cccaaagcaa tgactgatgt atttgtcttt tctttgaaat aatcagccaa
     1261 aaaatcaacg gcatggagag gttctgcctg ttttctcaag atcatcggga aatttgtttc
     1321 ccctaaacca tccataccat gagtgtcttg tgcactgatg aaaggttgct tcaaaggttt
     1381 ttccattcct tgataaacag gaatgtctaa acgatcacag cgttccaggc atttcagcac
     1441 attctgcgta cctatatctg cagggacatt cccacaaact acggtaatag caactacttc
     1501 gatatcaggg gatttgacag ctaatgataa agctaaagtg tcgtcgatcc ctggatcgca
     1561 atcaataatt acttttcgca ttatttccaa tctcctttct ttagagatac ttagtttttt
     1621 attctgggaa gtttccgaac cagtccaaga cgagcgtctt gattataatg cacgaaatta
     1681 gtatactggc ttttattgcg aatgtaaatg ctctcttaca agtaaatcta ttgaggagaa
     1741 ctttaagtaa atgatacaat gaaaaagaaa tggaatttac gtgtgccgaa aataaagaat
     1801 cctatggatc ggtacattgg ataatagata gattatagtt tggcagaaag cggcgggtgt
     1861 acaatggtta ttttttattt cattcttatt ctgataatta tagggatcag ttaccatttt
     1921 ctttcttatt tttatgcaaa gaaattagta agagcgggag tacagagagg atatgactgg
     1981 tatgaagaca ctggtcatcg tttgatggac cctgctgcag tcacgtcagt agaaaaaaaa
     2041 cgacaaaaat tagaagaaga gtgtgaaagc ttttgggagc agggaacacc caaaacaatc
     2101 aaaagttatg acgggttgaa actagctggt cagatgttca tccatccaaa taagcagaat
     2161 aaatgggtca tttgcgtaca tgattaccga agcacaggga aaagagacat gtctcatata
     2221 ggtaaaagat atgcagagaa aggattcaac gttcttgtac ctgatttacg tgcgcacgga
     2281 gaaagtgaag gagaaatcat cggaatggga tggctagatc ggctagattt aattgcctgg
     2341 atccaactca tcttagatga acagccgaat gcgtccatta ttttacacgg cggatcaatg
     2401 ggagcttcta cgatcatgat ggcaagtggt gaaaaacttc catcagcggt caaaggattt
     2461 attttagaca gcggatatgt ttctgtttat gcagaattcc gatatatgtt aagtaaaatc
     2521 actgtctttc ctaaaaaaat ggtcatgaga tatgcgaatc attatgccca gaaatacgcc
     2581 ggttactcct taaagcaagc atcagctacg agacagcttg gcagcaatca tctgccttta
     2641 ttagtcatcc atggtgaaag agatcatttt gtccctacag aagcagcgta cacgattcaa
     2701 aatgctacgg caggggataa agcactgctg cttgttccgg aagcagaaca cttggaagca
     2761 tcgatgaaag atccgaaaac ctattggaca gtgatttttt catttattga acaaagaata
     2821 acgatgaatt aattttctct tcttttttgt aagtaattcg aggataaata attgtttata
     2881 gatcaggaga ctgctaaaat aaggtaggaa taaaaaaata tcagttattt gaggagtgcg
     2941 gcatatggaa atcaaagaag aaaaaatcgt cttgctttat tcaatgatga acaacaagag
     3001 atcggagaaa tgacatggtc agatgcgggc ccagacatca tgatcgttga ccatacattt
     3061 gtagatccag catatcgtgg tcaaaagtta gctgaaaagc tagtgtatac tggagtagaa
     3121 ttagcaagac gagacgggaa aaagatcatt cctttatgcc cttatgcaaa aaaagagttt
     3181 gaaaagaaac cagaatatca agacgtctta cgtcagtcat aagaataaca gctgaaagtt
     3241 ttgaactttc agctgttatt ccatttatat agagttgtca cggactttta tctatgataa
     3301 agttaaagag acgatatctt ttgggaagga ggctgcgata tgtataattt aattttggga
     3361 atcgtgatcg ttttatcgat tgttatggtc attgcgatta tgatgcagcc aagcaagcaa
     3421 aatagtgcag cgagtgcatt taccggcggt gcggatcaac tattcggcaa acaaaaagct
     3481 cgcggctttg aagctgtcat gcagcgatca actgcagtta tgggagctat ctggatgatc
     3541 ttattatttg tcctagcata tttatcttcg aagtagttga ataagcctct cgcaaaacgg
     3601 cgggcggctt tttttattgc aaagagaacg cttaagaaga agttatgaaa gaaactatgg
     3661 taaaatgagg cgaaagaggt gagacgagtg agacaattac caaaacccct ttatgcaaaa
     3721 catggaaagc gagcggtttt gctgcttcac gcttactcag gaagtccaaa cgatgttcga
     3781 atgctcgcac gatttttgga aaaagcagat tatacagttt atgcgccgct ttttaaagga
     3841 cacggaacaa tggagccata tgatatcttg caagaaaaag cagaaagctg gtgggcagac
     3901 actaaaaaag ccatccattt tttacagtct gagcagtttt ctgatattgc cgtattagga
     3961 ttatcaatgg gcggcatctt tgctgtacga gcactagaag aagaatcagt gattggcggc
     4021 ggttttttct gttcgccttt gtctcctgta aaaacaaacg tgcctgaaaa ctttgaaaaa
     4081 tacgtacgtc aagtgctgaa gacagcaggt aaatcagaga aagaaatcaa tgaaaaagca
     4141 gttgcttacc gctcgttagc tgaacaacaa ttaatggata tacaagatca ggcagcaatc
     4201 gtagaaagta ggttgtcaga tatccaacag ccgatatttt tagctcaagc tgggaaagat
     4261 gaaatgatcg accctaatgg cgtatttgaa actgcacgca agttgagccg tcagcgagtg
     4321 acacttcaat ggtatcctga aagtggtcat gtgattacag ttggtacagc aagacgcgaa
     4381 ttagaaaaag acgtcttgga atttttagaa aaattacctt ggaatgagga ataaatatga
     4441 caaaagaaac gataaaagag aaaattctct ttttcatgga aagcagtcgc aaacgcagtt
     4501 tttctatgga agaaatcgct gaaggtttag ggttccaaaa aagcgatgat tttaaattac
     4561 tagtacaaac gattgctcaa atggaaagag aacagagtat agttttcaat aaaaaaggaa
     4621 aagtgaaact tcctttaaaa ccaatcctga tcgaaggaac atttcgagca aatgaacgag
     4681 gatttggatt tgtaacgatt gatcccgaag aagatgacat ctatatccca aaagaagcga
     4741 ctggttttgc aatggatgga gacaccgtag caatcgatat catgaagcga gcaaatacag
     4801 atatggatcg cggagcagag gggaaagtag tcgagattcg agaccgggcg actactcagt
     4861 tagcagggga attcgttgct tattcagaag aagaaataca agaaacagat ctttacggcg
     4921 ttgttatccc gaaagataga aagctcaacc aatttaaagt atatgcagca gcagagggag
     4981 ttcgtccagt tgacggaagt attgtgctga tcgaactgac ccattaccct gaaaaagggt
     5041 atgcgactag tttagaaggg ataatcaagc aagtgattgg tcataagaat gaccctggca
     5101 tggatatcct ctcaattgtc gtggctaatg gtatccctac gaaatttcct gatgatgtat
     5161 tgtctgaagc agatgaagtt ccagatcaaa tcagcgaaaa ggatataact ggacgtagag
     5221 atttaagaaa tcagctcatt gttacgattg atggagaaga tgcaaaagat ctagatgatg
     5281 cagttacggt taaaaaatta gataacggta attattttct tggagtacac attgcagatg
     5341 tttcttacta tgtgacacaa ggcagtcaat tggacagaga agcatatgaa agagggacaa
     5401 gtgtatattt gactgatcga gtaattccga tgatcccgca acgtttatcg aatggtatct
     5461 gttcactgaa tccccatgtt ccaaggctga ccatgagttg cgaaatggag attgatccaa
     5521 atggacaagt agtctctcat gatattttcc caagtgtgat ccaaacaaca gaacggatga
     5581 cctacacagc tgtcaatcaa atcttagaag atcaagacga agaaacgatg caacgctatg
     5641 ctcagctcgt acctatgttc caagaaatgg gtgaattgca ccgaatcctt gaaacgatgc
     5701 gaatcaaacg tggcgcgatt tcttttgagg atcgcgaagc gaaaatcctt gttgatgctg
     5761 aaggacaccc aaatgacatc cagctaagaa gcagaggaat tggtgaacgc ttgatcgaat
     5821 ctttcatgct tgcggccaat gaaacggtag ctgaacatta taataaatta aatctgccgt
     5881 ttatttaccg aatccatgaa cagccaaaag aagaaaaaat gcagcgcttc tttgattttg
     5941 cttcagcttt gggcattctg gtccgaggaa cgaaaggaac gatcacacca aaagatttac
     6001 agcatgtgat cgaaggagta gaagacaaac cagaagctgc agttatcaat actatgttgc
     6061 taagaagcat gcaacaagca cgctattcag aagacaattt tgggcattat ggcttagcgg
     6121 cagattacta cacacacttt acatctccta ttcgacgcta tcccgatcta atcgtccatc
     6181 gtctgatccg ttcttacagt gaagataaaa gtgaaagcaa tcaaagttcg tgggctgcgg
     6241 aacttcctga aattgcagat catagttcta agatggaacg tcgtgcagta gaaacagaac
     6301 gtgaagtaga ttccatgaag aaagcagaat tcatgatgga caaagtggga gaagaattcg
     6361 atgggatcat cagctctgtt gtgaaattcg gcctgtttat cgaactgccg aatacagtag
     6421 agggattgat ccatatcaat gaattgaaac aggactactt ccattttgtt gaaaatcatt
     6481 tagctcttgt aggtgaaaga actggtctga cttttaaaat cggacagaaa gtgcgagtaa
     6541 aagtagtcaa agctgatcca gatgaacgag cagtagattt tgaacttgtt tccgcagaag
     6601 aggttacagc gttggaacgg ccaaaaacac gaaatggaaa atcaggaaaa cgcgaaggaa
     6661 aacaagcagg aaaacgccca gaaagaaatg gcaagaagaa caaacgatat agcgatagta
     6721 agcaaacgtt cggtaagaaa aaagggaaga agccatttta caaaggtgtt aaaaagaaga
     6781 aaaagaaaaa ataaaatgag ttggaggaaa cagctatgcc aaaaggcgag ggaaaattaa
     6841 ttgcacaaaa caagaaagct cgccatgatt attcgatcat cgacacgatg gaagcaggga
     6901 tggtcttgca aggaaccgag atcaagtcga tacgaaacag ccggatcaat ctaaaagatg
     6961 gatttattcg cgtccgcaac ggagaagctt tcttgcataa tgttcatatc agtccttatg
     7021 aacaaggaaa tatttttaat catgatccgt tgcgcacgag aaagttatta ttacacaaaa
     7081 aacaaatcat ccggcttgaa aatgaattga aaaatactgg aatcactgtt gttcctttaa
     7141 aagtctatat tcgtaacggc tatgccaagg tattgattgg tctggcgaaa gggaaaaaat
     7201 cttatgataa acgggaagat ttgaaacgaa aagatatcga tcgacaaatt gatcgaacat
     7261 taaaaaattt ctctagataa tttcattatc ctttttcgca tattcggtgc ttttgctttt
     7321 acttaatgat aaaatttgtt agcatttaat ctgataaaac atgattatca taggtttttt
     7381 cttagatcag gagcaccgtg tataaagaaa aattcgcttc gtttttgact gaaataaaaa
     7441 agtgtctaat tttcatgaaa atgttttgaa ttttctgttt catggtggta tgataccgtg
     7501 tggttagaaa ccgattggaa aaaaccagat acgcacatga attgatttta tgtgaatttc
     7561 ggggaaaaga ggtgaaatgg attggatgaa cgctcgctaa cgttccacat tggaccaatt
     7621 tggtttgatg gtacagtttg tataatggtg ttactgacct gtttgattgt tttcttctta
     7681 gcttactttt ttacaagaaa tcttaagttg aaaccaacag gcaaacaaaa tgcgatagaa
     7741 tgggtcattg attttagtcg aggaatcgta acagataatc tgcctagaaa agaattgagc
     7801 aatttccatt tgttggcatt tacattgttt ttatttgttt ttgttgcaaa caacatcggt
     7861 ttagtaacaa aaatcgtttt acctaacgaa acaacacttt ggaaaagccc gacagcagac
     7921 ccgtttgtta ctttaacttt ggcatttata atgatcttac ttactcactt attcggggtg
     7981 aaaaagttag gcttcaaagg atatttcgta aattcgttct tgaaacccta tagttttatg
     8041 tttccgatga aactgattga agaatttaca aacttattaa cacttgcttt acgtctttat
     8101 gggaatattt atgcggggga agttttgcta accttgattg caaaaatgat gacaagctta
     8161 ggctggttct cattaccatt agcaattcca ttagagatgg tctggatcgc gttctccttg
     8221 tttattggga gcatccaagc atttgtcttt gtcactttgt caatggttta tatgagccat
     8281 aagattgaag tagaagaata atactcggtt aaaaaacaaa ctactattta tctattttag
     8341 gaggaaaaca attatgaatt atatcgcagc agcaatcgca atcatgggag cagcaatcgg
     8401 agccggttat ggtaacggtc aagttatctc taaaacaatc gaatcaatgg ctcgccaacc
     8461 tgaaatgtct ggtcaattcc gtacaacaat gtttatcggg gtagctctag ttgaagctgt
     8521 tcctatctta ggggtagtta tcgccttgat tctagttttt gccgtttaat tgaatcagta
     8581 ttaaggcaga aacaacatcg tttgttactg cttttttaca tggaaactag aaatactcca
     8641 aagaaaggaa ggctcgccta tgctgaatca attggcaatt gcagaagttg gcaatgcgat
     8701 gttaggtaat atcatcgttg tcagcggctc atttttgata ctactggctc tcttgaagca
     8761 ctttgcttgg ggaccaatca gcgatatttt gaaaaaacgt gaagacaaga tcgccaatga
     8821 tttagattct gcagaacaat ctcgcatcaa ctcagcgaaa atggaacaag aacgcgaaca
     8881 acaattgtta gcctctcgtt ctgatgcagc tgatatcatc aaaaatgcga aagaaagtgg
     8941 agaattaagc cgccaaaata ttttgaagga tgctcaagaa gaagcagctc gtctaaaaag
     9001 caaagcccaa gctgatatca ctgtagaacg tgattcagcg ctgaactctg taaaagacga
     9061 cgttgcagaa ctctctcttc aaatcgcgga aaaaatcttg aacaaagaat tatctccaga
     9121 aatgcatgaa tcattaatca atcaatacat tgaaggtcta ggttcttcaa atgaaactag
     9181 ataaatatac agtaggaaga cgttacggga aagctttatt tgaactggcg atcgattcaa
     9241 atcaagcaga agaaatctat caagatctcg taagtttacg tcaaatctat gaacaagtac
     9301 ctggattagg gaatatgtta agtgatgttc gtctagaacc tcatgaaaaa cgagaaatca
     9361 tggacaagct tgtcagcggt tatgatggga tcgttaaaaa ctttttagaa gttgtttatg
     9421 agtataaccg aatggatgac cttttgttca tgatcgaaga atatgaacgt cgatatgatg
     9481 aacatcaagg gttactattg ggaagtgtca caacagcagt cccactttca gacgaacaac
     9541 gaatacaatt agaaaagaat gttgcaaaaa caatggacta tcaatcagtt gaattaaaac
     9601 aaatcgttga ttcttctatt attggtggcg cgattgttga agccaaccat cgagtaatcg
     9661 acggaagcat tcgcacgcaa ttagaaaaaa tgcgtaatca attaaacaga tagtacaaag
     9721 aggtaggtga atcgaatggc catcaaagca gaagaaatta gtgccttgat taaagaacaa
     9781 attgaaaatt atcaaaacgt gctttcagtc gaagaaatcg gtaccgtaac ttatgtaggt
     9841 gacgggatcg ctcgtgcaca tggtttagaa aacgcgatga gtggtgagtt gcttgaattt
     9901 tcaaacggct catacggaat ggcgcaaaac ttagagtcaa atgatgtagg tatcattatc
     9961 cttggcgatt tcgagtccat tcgtgaagga gataaagtaa aacgaacagg taagatcatg
    10021 gaagttccag ttggagaggc cttgatcggt cgggtagtca atccgctagg tcaaccaatc
    10081 gacggactag gtgaaatcgt tactgacaaa gctcgtccag tagaagcgat ggcaccaggt
    10141 gttatgcaac gtaaatctgt taacgaacca atgcaaacag ggctaaaagc gatcgacgcc
    10201 cttgtgccaa tcggacgcgg acaacgtgaa ttagtcatcg gtgaccgtaa aacagggaaa
    10261 acttctattg cgatcgatac gatcatcaac caaaaaggtc aagatatgat ctgtatctat
    10321 gtagcaatcg gacaaaaaga ttctacagtt cgtacacaag ttgaaacatt gaaaaaatat
    10381 ggcgcaatgg attacacaat cgttgtgaat gccggtgcgt ctcaaccagc accattgctt
    10441 tatatcgcac catatgctgg tactgcaatg ggtgaagaat tcatgtacaa cggtaaacat
    10501 gtattgatca tttttgatga tctttcaaaa caagctgttg cttatcgtga actttccttg
    10561 ctacttcgcc gtccaccagg tcgtgaagct tatcctgggg atgttttcta cttgcactca
    10621 cgtttattag aacgtgcagc aaaattaagt gatgatttag gtggcggttc aatgactgcc
    10681 ttaccatttg ttgaaacaca agctggagat atctctgctt atatcccaac aaacgttatt
    10741 tcgatcactg atggacaaat cttcttagaa agcgacttgt tctacgcagg tactcgtcca
    10801 gcggttgatg ccggactatc tgtatctcgt gtaggaggtt ctgcgcaaat caaagcaatg
    10861 aaaaaagttg ctgggacttt acgcctagac ttagcaagtt accgtgaatt agaagcattt
    10921 acacaattcg gttctgactt ggatgcagca acacaagcaa aactaaatcg tggacgccgt
    10981 acagtagaaa tcttgaaaca aaaattgcat gcaccgctag ctgttgaaaa acaagtagtg
    11041 attttgtatg ctttgacaca tggattctta gatagtatcc ctgtagacag tattttagac
    11101 ttcgaacatg aattattcga atatctggat accaatcatt cagatatctt tgaaacaatc
    11161 cgtacaacaa aagatcttcc agaagaagaa gcgttgaaca gtgcgattca ggaatataaa
    11221 gacatgttct tagctacaaa aggaagtaat tcctctacag aagataaatt gaaatccatt
    11281 caaaatgcat agtgaggtga gatgaatggg tgcttcatta aatgaaatca agacacgtat
    11341 cgcttcaacg aaaaaaacga gccaaatcac gcgggcaatg cagatggtgt ctgcttctaa
    11401 gttgacaaaa tctgaagctt cttcacagaa gtttcaggtt tatgcaacaa aagtaagaga
    11461 aatcgttacg catttgactg ctactcagtt aaatgatatt gcctcagaaa atcctagagg
    11521 ggatatcaat tacaacagca tgctgctcag ccgtcctgtg aagaaaactg gttatatcgt
    11581 catcactgca gatggcggtt tagtcggtgg atataacagc tcgatattga aacaaacgat
    11641 gtctatttta gaagaagacc accaatcacc cgatgattac gtgatgatcg caattggcgg
    11701 aactggtgca gacttcttta aagcacgcgg tatcaatcta gcatatgagc tgcgcaatct
    11761 atctgatcag ccaagctttg atgaagtccg caaaattgta aatatggcaa caactatgtt
    11821 ccaaaacgaa gtattcgatg aattatacgt atgttataac catcatatca attcgctaac
    11881 tagtcagttt cgtgtagaaa aaatgctgcc aatctcagac ttagatccag aagaagcaac
    11941 gactttcgaa caagaatata tttttgaacc ttcaaaagaa gaaatcttgt ctcagttatt
    12001 gcctcagtat gctgaaagtt tgatctacgg tgcgatcgtt gacgccaaaa cagcagaaca
    12061 tgctgctggt atgacagcaa tgaaaacagc aactgataat gctgctaata tcattgatga
    12121 gctgacggtt tcttataacc gtgcaagaca aggggctatt acccaagaaa ttaccgaaat
    12181 tgttgccgga gcttcggcac tagaatagta ttgagattgg aggaaaaaag atgagttcag
    12241 gcaagattgt tcaagtaatc ggtcccgttg tcgacgtgga attttcttta gaccaatcct
    12301 taccagatat caacaacgca ttagttgttt ataaaaatga cgaaaataaa tcaaaagttg
    12361 ttcttgaagc agcgttagaa ttaggggacg gagtgatccg gaccattgcg atggaatcaa
    12421 cagatggttt acaaagagga atggaagtca ttgatacagg caaagcaatc tctgttcctg
    12481 taggtaaaga aacattaggt cgcgtattca acgtactagg agatacgatc gatttagaaa
    12541 cacctttccc agaagatgcg gaaagaagcg aaattcataa aaaagcacca gcctttgacg
    12601 aattaagtac aagtacagaa attttggaaa cagggatcaa agttatcgat ttgcttgccc
    12661 catatttaaa aggtgggaaa gtcggactat tcggtggtgc cggtgttggt aaaaccgtac
    12721 tgatccaaga actgatccat aatatcgccc aagaacatgg tggtatttct gtatttaccg
    12781 gtgtaggtga acgtactcgt gaaggtaatg acttgtatta tgaaatgaaa gattcaggag
    12841 ttatcgaaaa aacagccatg gtgttcggac aaatgaacga accaccaggt gcacgtatgc
    12901 gtgttgcttt gactggtttg acgattgcgg aatatttccg tgatgtagaa ggtcaagatg
    12961 tactgttgtt tatcgacaac attttccgtt tcactcaagc tggatctgaa gtatcagcct
    13021 tgttaggacg gatgccttct gcggttggtt atcaaccaac attggcaaca gaaatgggtc
    13081 aattgcaaga acgtatcaca tctacgaaaa aaggttcgat cacatcgatc caagcaatct
    13141 atgtgccagc cgatgactac actgacccgg cgccggcaac agcattcgcc catttggatg
    13201 cgacaaccaa cttggaacgt aaattaacag aacaagggat ttatccagcg gtagatccat
    13261 tggcttcttc ttcaagtgct ttagcacctg aaatcgtggg agaagaacat tataaagtag
    13321 caacagaagt ccaacacgta ttgcaacgat atcgtgaatt acaagatatc attgctatct
    13381 taggtatgga tgaattatcc gatcaagaaa aagtgttagt ttctcgtgcc cgcagagtcc
    13441 aattcttctt gtcacaaaac tttaacgtgg ctgaacaatt tactggtctt ccaggttcat
    13501 atgtccctgt agaagaaaca gtgaaaggat tccgcgaaat cttggaaggc aaatacgatg
    13561 atcttcctga agaagctttc cgaagtgttg gccgaatcga agacgtcgta gaaaaagcga
    13621 aaacactagg ctactagaaa ggggtgcccc atggatcaat atttaacagt aaatgtggta
    13681 actcctgccg gtttggtcta tgatcaccat gcagctatcg tcgttgcacg tacgacagct
    13741 ggtgagcttg ggattttgcc taaacatgca ccaatcatcg ttccattgac gattgatgaa
    13801 gttcgtgtaa aacgaacaga ttcagatacc cacgtcgatt ggatcgctgt taatggcgga
    13861 atcatggaag ttcgtgataa tgttgtttca atcgttgctg atagtgcgga acgtgaacgt
    13921 gatatcgatg tatctcgtgc ggaacgtgca aaacaacgcg cagaacgcca aatcgaagaa
    13981 gcaaaagcaa aacaagatac gaacgaattg aaacgggcaa ctgttgcgtt gcaccgagca
    14041 atcaatcgta tcaaagtatc taagcactca taaaatgaga tgcggatgtt tatccgtgtt
    14101 taagaacagt aaaaagaggt tatgacagaa gtggtcagct tcaagacata agaaggaatt
    14161 tacgaaaatt gcttctcaaa tttttgtgaa atctcagctt atttttcgaa gaagctactt
    14221 ctggaacacc gtatatcaga ggttgtgaaa tcagcgtttt gtcctgagta ttagagaaac
    14281 aaatggaaaa tagttcctca tattttgccg ttttttgagc taataccgag ggtcagatga
    14341 ttgaacaccg tttatttgga ataagggact gcgacaagac ttttgtctca gtcccttttt
    14401 ttgcatattt ttagcattta tttcctaaaa aatgtaaggg gattcctata tttttaagta
    14461 tacttgtttt tccttttata cattttatgg tattatcatt ttattagtta ttataaaaca
    14521 atcgttagaa aggacgtcat tatgcaagta tacggaattg atgcggtgct tcgaatcgtg
    14581 agtcatttgg cgtttatcta cttagccttc tggtctttac gttcgttacg cattgaaact
    14641 tttttcagac caatgcataa tacgcaaatt agaatggcaa tcgtactttt ttcaattttt
    14701 ttagggtaca ctgcaagtaa ttttttctta gaggttatcg ctttatgccg aaatcttttc
    14761 gttagcctgt aaattttaaa aaaaggtact attttaaaag caaggatgtt ataatagaaa
    14821 agattttgag taagtgtatg ctcattggaa ttttttggag ggaacaacat ggaagagatc
    14881 atcgtaagag gtggcaatca attaaatgga acagtaagaa ttgaaggagc taaaaatgct
    14941 gttttgccta ttttagctgc tagtttgtta gcagaagaag gtattacgac attagacaac
    15001 gttccgattc tttctgatgt cttcactatg aatcaagtga ttcgtcactt aaacgtagat
    15061 gtagattttg atgaacaaaa gaatcaagta acaatcgacg cttcaagaca attagaaatc
    15121 gaagcgcctt atgagtatgt tagtcaaatg cgtgcttcaa tcgttgtgat gggtccgcta
    15181 cttgccagaa atggacatgc aaaagtagct atgcctggtg gatgtgcgat tggtaaacga
    15241 ccaatcgatt tgcatttaaa aggatttcaa gctttaggag caaaaattat tcaaaaaaac
    15301 ggctatatcg aagcaattgc ggatgaattg atcggtaaca cgatttattt agatttccct
    15361 agtgtaggtg ctactcaaaa tatcatgatg gctgccgtaa aagcaaaagg aacaaccatc
    15421 attgaaaacg tagctcgcga accagaaatc gttgatttag caaacatttt gaataaaatg
    15481 ggcgctcaag tctatggagc tggtactgaa acaatgcgta ttgaaggcgt ggatcattta
    15541 catgcagtca accactcaat cgtacaagat cgtatcgaag caggtacctt tatggttgct
    15601 gctgcaatga cacaaggcaa tgtgctgatt gcagatgcta tctctgaaca taaccgccca
    15661 ttgatttcta aattgatcga aatgggtgca gaaatcattg aagaagaagg cggagtccgt
    15721 gtcatcggac caaaacatat cttacctaca gatgtaaaaa cgatgcctca tcctggtttc
    15781 cctacagata tgcaagctca aatgacagct atccaattag ttgcagaagg tacaagtgtg
    15841 gtgactgaaa cagtgtttga aaatcgtttc caacatttag aagagatgcg ccgtatgaac
    15901 gctcatgtca aaatcgacgg aaatgtagca atcatggatg gaaaccatga attgcaaggt
    15961 gcagaagtat atgcaacaga cttgcgggca gcagcagcat tagttttagc tggcttgaaa
    16021 gcaaatggaa tcacacgtgt gcgtaacttg aattatctag accgtggata ttataatttc
    16081 catatcaaac tacagcaatt aggtgcagac gtcgaacgtg tagacatgga tcaaacttct
    16141 gctgaaaaaa cagcacaaac gattgcttaa ggaagtggct gaatgagtaa ccaacgatat
    16201 atcttattaa ctttgataaa gattttagtg gtgatcttat tgcttatcct tctttttgtg
    16261 gccggaacga tgatcggata tggtgtcatc ggtggcggca atccatttaa agtattccag
    16321 ccaagtttgt ggattcatat tcgagacttt tttcattgat aaataagaat cacagtggtt
    16381 ctagtcgata tgaagtgagc catgaagcag aacatgataa aagaaaacag aagggaacgt
    16441 tgacgttagt cgcgatccct ttttttagta aaagaaaaga aggaatctaa ttgatgaaga
    16501 agatattgat ttatttagtt agaggatatc agcgctttat ttcgcctgca tttccaccta
    16561 gctgccgtta ttacccaacc tgttccaatt atatgataaa agcaattcaa acccatggtg
    16621 cgctaaaagg gacggtaatg gggactgcac ggatcttacg ctgtcatcct tttgtcaaag
    16681 gcggtatcga ttatgtacca ttgacattcc gattaacgcg aaataagaaa gataaaaaag
    16741 aaccagatta taaaaaggtt gtgaaaaata cgggtagcac tgagcgataa agaaaaagag
    16801 gaaatttatt gtcaaagtaa caactccgca actacttata tgggggacaa aaaatgatcg
    16861 aagaactaaa caagaaagat ttaccatggt ccttgttatt agatgcagat cctgataaag
    16921 aaaaagttca agtatatgtt tccagaggga gtggctggat atggaaagaa aaagaaaaaa
    16981 caataggtgt actgatctat gtggcaaggg aaaaagaata cgaaatcgtc aatgtagcag
    17041 taaccccaac gtatcaagga aaggggattg gcggaaaatt acttgagaca gcttttcaga
    17101 agctttcaca gctagcttca aaccaaactc ggatcattat acgtactggc actactagta
    17161 gcgctgcgtt acacctctat caaaaaatgg gctatgtgga gattggtcga gtcaaagact
    17221 attttatcca tcattactct gagcccattt ttgaaaatgg agatcaatta agggatcaag
    17281 tgattttagc aattcactta cctaaaaata gctttaacta ataaactttc aattcgagaa
    17341 aatgctttca aatgctaaat agatacatta caatagcttt agcaaacaaa aaggagaata
    17401 tttttatggg aatattagtt tttgattttg gtggatcggc tgtcaaatat ggttgttggg
    17461 atggcaaaga gattaaaggt aagggacaat ttgcaacgcc tgaatcttgg gaagaaatga
    17521 aaacgcaatt atttcaagtt tataaaaaaa tactcgtttc ttttgaagtc gaaggaattg
    17581 gaatcagctc tcctggagta gtgaataatg agaaacaggt gattgaggga atcagtgcga
    17641 ttccttatat tcatggattt aatattttcc gagatttgga aactctgttt caactcccag
    17701 taacgattga aaatgacgcc aactgtgccg gaatggctga attttatgaa ggtgctgccc
    17761 gtgattatca agatgttgct tttgttgtgg caggaactgg gattggcggg gcgttgttca
    17821 cgaaaggaca aataaataaa ggggcacatc tttacggtgg agaatttggg ttgatgttct
    17881 tagatggcga taagacattc agtaaacttg gtacagcggt acagatggct tggcgttatt
    17941 gtgaacgcaa aggtctcgat aaaaatgctt ataccggaaa agatgtcttt gaattagcag
    18001 aaactggaga tgcaatagca aaagaagaag ttgagagttt ttatacgtat cttacaaagg
    18061 gattattcag tattcaattt tcgtttgatc cagaagtgat cgtattaggc ggcggggttt
    18121 ctgcaaaaga agggctcatt gatgagatca aaagccgaat gaagaagctg acagaacaat
    18181 tcgatctgca tgatttcgaa ccgcagattt tattatgtga gtacagaaac gatgctaatc
    18241 ttataggtgc agcagcaaac tatctcgctg tacgtcaagg agagaattaa gagctttaga
    18301 gaaggtgcag cttgcataaa agaagagaat gaaatgaata ggagattttt acctaaggaa
    18361 tgttttattt gctgcagtat gataaaatat ctgagaggtg aaaatgatgt caaaaaaaaa
    18421 taaagatatt gaagtgcgta tagaagaagt gaaaaaagat atcaaaggta agaattatga
    18481 agtcactcag ttatttatag ggaaaaaaat gattggtgag atcttagcct atgggccaaa
    18541 agaatatgaa attttctttg gtgaagaaga ttttggaaaa gaaaaaagtc ttgaaaatgc
    18601 gatagaaaca gtgattcgac actggaatct gcacgaataa aaaaatatca aaaaaacttt
    18661 caataaaggg ttgcaaaaat ctggaaaatt aggtattata actaagtcag gttgatacaa
    18721 acctttcgga ggagtagcga agtggctaaa cgcgacggac tgtaaatccg ttccttaggg
    18781 ttcagtggtt cgaatccact ctcctccatt tcattggggt atagccaagc ggtaaggcaa
    18841 gggactttga ctccctcatg cgttggttcg aatccagcta ccccagtata ccacaatcgt
    18901 aagctctcgt tagttcgaga gctctttttt tttgctttgc taccttgttc cttgaagatt
    18961 ctgctgaatt aagatagagt taagttaact acaagaattg gagggatcca catgaaatgg
    19021 aaagaaaaag tagacgaagc agctgaaaag ttatatgatt tggtcaaatc agaaaaatac
    19081 aatattgaag tgaatattcc gaaaaaaggc ggaaaagcgg tccaggtaaa atcaaaacgc
    19141 ccaacgaatc atacaaaaaa atggatggct aaaaaccgct aataataaca aaagatccat
    19201 aagtcggcat tttcatcgat tttagggtct tttgttattt tctaggttga acatatctaa
    19261 aaaattgttt ctgacaatag gagtagggag aacgatgtgt gtctaaatcc tctgttgatg
    19321 gatagaaacc tttatctttt tcagttgtga atcaggtata taccagttat aatagaagaa
    19381 acgaaaggac gggtgtacgt tatgtcgaat caattacctg tatatataca gattcatgac
    19441 caattaaaaa atgaaatcga acaaggtata tggaaagtcg gagaccgtct tccttcagaa
    19501 agagaattag cagtgaaatt caatgtcagc cgtatgacgt tgagacaagc aattcaaaac
    19561 ttggcagatg aaggaatctt agaaagaaag atcggttcgg gtacgtatgt tgcgcgagaa
    19621 aaagtacaag aaaaaatgtc tggtgcaaca agttttacag aaattatgga atcacaaaat
    19681 cgagtaccgt ctagtcggac aatctcttat ttcttgactt cgccaagctc aagtgaaatg
    19741 gaaaaactaa acttgagtaa agatgacacg atcttgcgaa tggaacgtat tcgtttcgca
    19801 gatgatgtcc ctatttgttt tgaagttgcc agtgttccac aaaagctgat cgatggctac
    19861 agcaaagcag agattactga atcgttgtat cgcacgctag aagaaaaagg cgggaaaaag
    19921 atcggtccag cgaatcaaac gattactgct gtgttggctt ctgagaaaat tgctgactat
    19981 ttagaggtaa aacgaagaga tgcagttctt cgtttgcgcc agatttctta ttttgaagac
    20041 ggtacgccgt ttgagtatgt gcgtacgcaa tacgtaggaa atcgttttga gttttatttg
    20101 gaaaaataaa aaacaggtga tccctgattc cttattagaa ataaaagaga atcagaatca
    20161 cctgttttta tatatgatca gttatcagtt gctcgtgata agaaacagaa ccctcttcaa
    20221 attggacttg tccgttcagc agctcaatga tggattcttt aaagtggtta gcactttttt
    20281 catctacagc acagatgacg ctaacttgat cagtatattg gatttctttt ataaaaaaat
    20341 gttggagttc aagataattc tgtaattttc ctagttgcgg atacgtgatc atcacagaga
    20401 tctcctgttg caaggtacct tctacgattc caatgtcaga caatgcttga gagaccgcat
    20461 gagtgtatgc tcgtattaat ccgcctgtgc cgagtttcgt tcctccaaaa tatcgagtga
    20521 ctactgcagc tacattgatc agttgatttt ttttgaggac ttccaacatc ggtacgcccg
    20581 ccgttccact tggttctcca tcatcgctgc ttcgctggat ttcatttttt tctccaatga
    20641 tgaaggcgct gcagttatgg ttcgctttcc aatgttcttt ttttatttct tgaatgaaat
    20701 tttttgcttg gtcctctgat tctattcgtc taagagaaca gataaagcgt gattttttga
    20761 tctcaatctc actttgtcca ttttctttta ttgtacgata gcgctcgatc atcttgtcac
    20821 ctcttttttt cattttcttt attcagtata aataaagttc taggttgttt caacactgta
    20881 acggttgcgc aattgctgta gtatgcgata ataaagacaa aggcaggtgg atctggggat
    20941 gcaggaacga atgaaaaagt acgatgcaat tacgcattat ttgaagaaca atggcggctc
    21001 tcaagtaacg ttgaccttta cccaatttga cgaactgctg tttcccagta acggacttcc
    21061 caaaacagca agggaaagta cagattggtg ggcaaatgat tataaacacc ctgaaaaagg
    21121 tgcctatggc tggataaatg caggctatga agtagttgtc atcaatttgg acaaagaata
    21181 tgtcgtgttc aataaattag tgaaatctag ttggctattt gattaaataa accgcgttta
    21241 gaaaacgttg acaaatttct ttctttttat ataataaagt aacagacgcg aagaaaacgt
    21301 ttgcgtagtt tgttttaaaa aggagagaaa aatatggcta aaaaaacaat catgcttgta
    21361 tgttcggctg gtatgagcac aagtttacta gtaacaaaaa tgcaaaaagc tgctgaagca
    21421 aaaggaatcg actctgatat tttcgctgtg tccgcttctg acgcagataa caatcttgca
    21481 aataaagacg tggatgtgtt gttattaggc ccacaagttc gctttatgaa agctgatttt
    21541 gagaaacgtc tggaaccaaa aggcattcct ttagatgtca tcaatatggc tgattatggt
    21601 atgatgaatg gcgaaaaagt gttagatcaa gcaatcgcgc tcataggtga ataaatagaa
    21661 cagtataact atcgaacaag cgatccaaaa aatagatcgc ttgttttttc gtatattttt
    21721 ttagggcgaa taatatgaat catttttggg gtagaaggct tctgacgaga gatattaaag
    21781 caatagactg tgaggaggta aatttaccaa attatgagaa gatcgcagca gtggaacgtt
    21841 tgggcaactg gattctttgt catcgctgtg gaatgaggac gtccgtcatt gttggacagc
    21901 ttgcagatta cggttatttt tgtacacatt gtctatattt agggtgatgc gacactcaac
    21961 aagatttata tttgtttgat cagcctgaag caaaacctcg agaagtcgtc tttttttgga
    22021 caggacagct tactgaaaaa caaacggaaa tcgcaaagag aatactttgc cattcagaaa
    22081 aaagacatca tcttatttgg gctgtaacag gtgcagggaa aacagagatg ctttatccta
    22141 ttttagtgga aactttaaaa gctggaggaa gagtagcgat ttgtacaccg cgagtcgatg
    22201 tttgtaatga attgttttta cgctatcgtt atgtttttcc agaagaaaaa atcacgttgc
    22261 tccatgggaa tacagcaacg gcttatacct ttacttcttt tgttatctgt acgattcatc
    22321 agctataccg tttttatcgg tcctttgatt taatagtgat cgatgagatc gatgcgtttc
    22381 cttataacgg ggatgctggg ctagctcacg ctgtgcaaac agcaatcaga tcaaacggcc
    22441 gattcatcta cttaagtgcc acgcctgatg agaagttact aaaggaaatc aagcagacat
    22501 ttgagctcca caaacttgcc ttgcgctttc atcggcgctt gcttcctgaa cccgtgcttg
    22561 ttttcatgaa taattggcga caaaaatgtg gaaacaaaaa gaaaatcaac tctttggtgc
    22621 ggatcattca gcaactaatc aaagaaaacc gtatcctttt attttgtcca agtattacga
    22681 tgatgcatcg tttaaaaatc acactgcaag aagcttttcc agaatatgag ataacagatg
    22741 tttcttcaca agacgatcaa cgggcaaaga aagttcagaa aatgcgagaa gcaatatacg
    22801 agatactgtt aaccacaatg atcctagaaa gaggagtcac atttgaaaga atctctgtca
    22861 tcgttttagg agcagatcat ccagttttta ctaaatcctc tttggtgcaa atcgcaggaa
    22921 gagctgatcg taaaggtcct tatacgaaca gtcgagtcta ttttttctat gaagaaaaaa
    22981 cttcagctat tcgaaaagcc tgtcaggaaa tcaaagaaat gaacgaagaa gggaagcaga
    23041 tgctgtgaga tgtagttgct gcggtaaaga catcattcaa aatttaacat tcagcgagat
    23101 catcggagtg tcgaaatcta gacttcctca gacatgttca gtttgccgcg cacaattcca
    23161 tttattagaa gcaaaaggtt gtgcaggttg tcaaaaacca atccatttga aatacgagga
    23221 gacgaaaaag aaagcaaata ctagtttcgt ttactgcaac gactgtcaag aatggaagaa
    23281 gaaatatcct gcatatgatt ttcaacaccg cgcctttttt acgtacgatg aaaagatgca
    23341 ggcttggatc aatcggtaca agtttatggg agatatccga ttagcaggga cttttgcata
    23401 taaatggact cagataaaaa aaagattacc cggcatatat ttattgtcca atacctttat
    23461 ctgaaaaaag gtggctagaa agaggattca atcaagtaag cgagatgttg aaagcagcaa
    23521 aaattccttt tcagatgctg ctcaaaagag agcaacatct gcttgctcaa gcaaaaaaca
    23581 cgcgaaagga aagattggag atgcctcagc cttttgagct tgccgtacct ctttctaaga
    23641 tacaacataa aaaa
//
LOCUS       DAYYGO010000035        23484 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000035 DAYYGO010000000
VERSION     DAYYGO010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.035"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..337)
                     /locus_tag="ACHA5T_001149"
     CDS             complement(<1..337)
                     /locus_tag="ACHA5T_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFM8886735.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQ"
     gene            610..3081
                     /locus_tag="ACHA5T_001150"
     CDS             610..3081
                     /locus_tag="ACHA5T_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8886736.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(3184..4920)
                     /locus_tag="ACHA5T_001151"
     CDS             complement(3184..4920)
                     /locus_tag="ACHA5T_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8886737.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4917..5621)
                     /locus_tag="ACHA5T_001152"
     CDS             complement(4917..5621)
                     /locus_tag="ACHA5T_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8886738.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5752..6255
                     /locus_tag="ACHA5T_001153"
     CDS             5752..6255
                     /locus_tag="ACHA5T_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFM8886739.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            6215..6553
                     /locus_tag="ACHA5T_001154"
     CDS             6215..6553
                     /locus_tag="ACHA5T_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFM8886740.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6574..7992
                     /gene="ffh"
                     /locus_tag="ACHA5T_001155"
     CDS             6574..7992
                     /gene="ffh"
                     /locus_tag="ACHA5T_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFM8886741.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            8104..8682
                     /locus_tag="ACHA5T_001156"
     CDS             8104..8682
                     /locus_tag="ACHA5T_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFM8886742.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8686..9207
                     /locus_tag="ACHA5T_001157"
     CDS             8686..9207
                     /locus_tag="ACHA5T_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFM8886743.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(9286..9840)
                     /locus_tag="ACHA5T_001158"
     CDS             complement(9286..9840)
                     /locus_tag="ACHA5T_001158"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFM8886744.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            10093..10368
                     /gene="rpsP"
                     /locus_tag="ACHA5T_001159"
     CDS             10093..10368
                     /gene="rpsP"
                     /locus_tag="ACHA5T_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFM8886745.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            10380..10631
                     /locus_tag="ACHA5T_001160"
     CDS             10380..10631
                     /locus_tag="ACHA5T_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFM8886746.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10756..11547
                     /locus_tag="ACHA5T_001161"
     CDS             10756..11547
                     /locus_tag="ACHA5T_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886747.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11717..12238
                     /gene="rimM"
                     /locus_tag="ACHA5T_001162"
     CDS             11717..12238
                     /gene="rimM"
                     /locus_tag="ACHA5T_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFM8886748.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            12228..12989
                     /gene="trmD"
                     /locus_tag="ACHA5T_001163"
     CDS             12228..12989
                     /gene="trmD"
                     /locus_tag="ACHA5T_001163"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFM8886749.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            13125..13472
                     /gene="rplS"
                     /locus_tag="ACHA5T_001164"
     CDS             13125..13472
                     /gene="rplS"
                     /locus_tag="ACHA5T_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFM8886750.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            13845..14507
                     /locus_tag="ACHA5T_001165"
     CDS             13845..14507
                     /locus_tag="ACHA5T_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886751.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            14586..17813
                     /gene="fss3"
                     /locus_tag="ACHA5T_001166"
     CDS             14586..17813
                     /gene="fss3"
                     /locus_tag="ACHA5T_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFM8886752.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITGAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            17927..18241
                     /locus_tag="ACHA5T_001167"
     CDS             17927..18241
                     /locus_tag="ACHA5T_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM8886753.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            18254..18628
                     /locus_tag="ACHA5T_001168"
     CDS             18254..18628
                     /locus_tag="ACHA5T_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM8886754.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            18638..18982
                     /locus_tag="ACHA5T_001169"
     CDS             18638..18982
                     /locus_tag="ACHA5T_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFM8886755.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDVEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            19053..20405
                     /locus_tag="ACHA5T_001170"
     CDS             19053..20405
                     /locus_tag="ACHA5T_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFM8886756.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            20465..21607
                     /locus_tag="ACHA5T_001171"
     CDS             20465..21607
                     /locus_tag="ACHA5T_001171"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFM8886757.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            21632..21886
                     /locus_tag="ACHA5T_001172"
     CDS             21632..21886
                     /locus_tag="ACHA5T_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886758.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            22142..23326
                     /gene="mobT"
                     /locus_tag="ACHA5T_001173"
     CDS             22142..23326
                     /gene="mobT"
                     /locus_tag="ACHA5T_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFM8886759.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            23323..23460
                     /locus_tag="ACHA5T_001174"
     CDS             23323..23460
                     /locus_tag="ACHA5T_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFM8886760.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
BASE COUNT         7789 a         4179 c         5038 g         6478 t
ORIGIN      
        1 cctgcttcag ttcctttgtt agttgatgat gttcatcaac taagtccgtt ttcgcagaaa
       61 aggtccttcc acaatcatgg cagagaaaac gggtacgttt taattctaaa atagttaact
      121 tttctttgac tttcaacatc tgagtgcgtg tcgtatagga accgttgcgg ataatacgaa
      181 ccgatcgaca gtgttcacaa taggaagaag agtcatccaa ttttccttta ataaactgcg
      241 cttgaacgcc tttatattct ttttcaatca gccattcttc atcaaaaaat aaatgtttat
      301 ctgttaatcc gagtaatttt ctagtatgat tatccattga gagactcctt tggtaggatg
      361 acgttttggt cgactaaatt ttaccaaaag tgagtctctt tttgagttga aacgaagaaa
      421 aagtgttagt agaaaatcgc cttcgatttt ccaccaacac aaaatattat agaacccttt
      481 ttttttgcta tatgacagga atgaaaggtg tttgcaagct taatgtgagg aacactttcc
      541 atatctgttc tatactggat aggagaagtt tggaaatatc cagaaaacga aatcaatcga
      601 ggtagccaca tgaagaataa atggataaat cgaagtaaaa aaaagaaagt aacacaaaga
      661 tcatcagata aagtcatccg tctttttcca tcgtataaag aaggattaac ggcagatcaa
      721 gtcaaggaac gaatagaaaa aggtgcagca aataattcag tcgatcccac attcaaaaca
      781 aatcagcaaa tcgtattgga aaacatattt acgtatttca atttgatctt tcttatctta
      841 gccattttgc tttgtttagt agaatcctat aagaacttga cttttttacc agttatcata
      901 gccaatacag gaatcgccat ttttcaggaa attcgatcca aaaagatctt ggacgaacta
      961 aatgttttaa atacagcaaa agtcaatgtc atacgagacg ggatagaaca aacaatagat
     1021 atagaagacc ttgtaataga tgacattgtt attttagaga ctggatatca aattccagca
     1081 gatgcagagg tcgtggaagg gaaactgcaa gttaatgagg ctttattgac aggcgaagca
     1141 gatgaaataa ccaaagaaat aggtgatcca ttaatgtccg gcagtttcat tgtttccgga
     1201 aaagcttacg cccgattaga caaagttgga gcagattcgt atatttccca gctgacagtc
     1261 aaagccaaga caatgggaga aggagaacag tcggagatga ttgcttctct taatcaattg
     1321 atcaaatgga ttgggatcat cattattcct atcggtatcc tgcttttttc tcaaagttac
     1381 ttttttaatg gaaacacgat caaagaaagt gtcgttgcaa tggaagcagc attgatcggt
     1441 atgatacctg aaggactgta cttgctgacg acgattgcat tagcattaag tgcgactaaa
     1501 ctagcaaagc aaagagttct tttacacaat atgaaaagca ttgagacatt ggcaagggta
     1561 aatgtccttt gtgtagataa aacaggaacg atcacagaaa ataaaatgtc tgttcaaaaa
     1621 atgatcgtct ctaaaaaaca agaaataatc agtcagtcag atcaattgga agcatgcatt
     1681 gctgactatg caaaagcgat ggcaggagac aatgcaacaa tggaagctgt taaagcatat
     1741 tttacaaaaa ccactgatca atcctatcac catgttattc ctttttcttc tgtccaaaaa
     1801 ttcagcagtg tagcgttggc agacaaggtg tatgttttag gagcgcccga aatggttttg
     1861 cgcgatcaat tgccggaata tgcggaagag tttgtttctt tcgctgaaaa aggctatcgc
     1921 gtgttggtct ttggtgtgta cgatgggatt cttgaagaac cagtattgac agaagcagtc
     1981 acaccattag gctatattct gattgctaat cctatcagaa aagaagcaag agccacattt
     2041 gattacttca aaaaacagca agtagccatc aaagtgattt ctggagataa tccgctcacc
     2101 gtatccaatg tagctgtcca agcagggatt tcacatgctg atcagtacgt tgatgcatct
     2161 aaattatcag aagaagaata tgaagcagct gtcgaaaaat atacagtttt tggacgcgtc
     2221 aagccagaac aaaagaaaat cttcgttcaa ttgataaagc gtaacaatac agtagctatg
     2281 acaggtgatg gcgtgaatga tattctagcc atgaaagaag cagactgcag tatagcgatg
     2341 gcttccggaa atgaagccgc catgcaggcc tcacaagtgg tcttacttga ttctgacttt
     2401 tcacgcatgc cagaagttgt tgcagaagga cggcgcgttg tgaacaatat tgagcgatca
     2461 gccagcttgt ttttagtgaa gaatattttc tcctttttgc tctcactgtt ttcagtgatc
     2521 tttgctctga cctatccttt agaaccatca caaatcacat tgattagttt gtttactatc
     2581 ggacttccat ctttcttgct tgctctagaa gaaaacaaaa agcgaatcag agggaaattc
     2641 atcatgaatg tgatggaaaa agctgtgcca ggtggactaa cagatatgat cgtagtcggt
     2701 gcgttggtga tctgcggggt gactttgaat ctaaataaaa cagatgtttc tacagcatct
     2761 accatgcttt tgattgcagt agggttcctc gttctctata aaatctgttc accgctgaat
     2821 aaattcagaa gtcagatcat tcttttctgt gctagcggta actttttttc ggtcatcttt
     2881 ttgcataagc tgttttcaat caccggtatc tcagctgtat cacttttgct gttgagcatt
     2941 ctatttttct cggcagattc gatttttagg catttgacca cattggtaga atatttgttt
     3001 gaacgtaaaa atgaaaaaga gcaaaagccg tttagtcgcg tagctaaaaa atttctatcg
     3061 atatttaaac gagaaaattg acgaaaagta tctcaaaaaa gagtaaaaaa gaacaaccac
     3121 tcgtgtatct ttcgaagcag gatgagtcat ccaactttaa aacgcacgag cagttgctct
     3181 tttttacatt ttcggaatgg taacggtgaa agtcgaacct gttccaagat gactgtctac
     3241 agtaatcgta ccattaagga gttgcacgta gtcttttaca atcgctaacc ctaatccggt
     3301 cccgcctgaa tgtcggcttc gcgccttgtc tacgcggtaa aaacgctcaa agatccgttc
     3361 ctgatcctca atgtcgatac ctataccaaa gtcttggaca gaaaacgata gatcatctgt
     3421 tgcctgatag cggataataa tctcgctgtc gctttttgaa tattggacag cattttcaat
     3481 tagatttttg ataattggat aaaggatatc tgttttagta gtaaatggtt cgttctttcc
     3541 aatcaatcga atcgtcaacc gtttcttttc gatgattggc tgataatttt gaacgatttg
     3601 ctggaaataa ggttctgtat aaattgtctg gatctcgtaa gaattctcgc catcttttga
     3661 cagctggatg atttcccgaa tcagtttatc taaccggatc gcatcttttt gcatgatctc
     3721 aagaaaagaa gtcagagttt gcgggtcatc ttttgcacca tccagtaaag tctcggtaaa
     3781 accaatcagt gaagtcacag gagtttttaa ttcgtgagag acgttgccaa cgaaatcctt
     3841 ttgtagcttt tctaaacgtc gcactttcgt taagtcatac gccaccccta gaatctgtcc
     3901 ggtaccttct gaattagtaa agtagcgcaa actcaagtct aatgtctgcc gaccttctgt
     3961 aagggtgatc tcctgatgga tcagcggatg ctcaggagtc acttgatgaa tcagctgaat
     4021 gagttttggc tcttgaatga cttctgtgta ccgctgttca ggttgatagc ttaccacgtt
     4081 taaatgtagc tgcatctttg gattaagcag tagtaaacga gaatcagcat catcaatcag
     4141 gaatacgcca atcatcagtt cgttcagtaa cgtatataat tgctcttctg tggtagtaca
     4201 cgcccgatac atcttactca tttgttcgct caacgcatta atcgtctgat acaattcttc
     4261 ccattgatcg ggtgtttgca tgattacttc cgcctgatta ggattttgca ccattttttt
     4321 tagtacgggc agcacggttt tcaatggctg gtttttttga tagatcagat aatagatcat
     4381 tcctgcaaga acaaggaaga aagacagaaa gaagaaaaag acccaacggc gaaaactctc
     4441 agttctcggt aaaaaaccgc ttgtcggttc tgcgatacgt aaaatggctt ctaactggcc
     4501 actttttttg actggtaatg cgacatataa aagatcctca ttcaaagtag tacttttgcg
     4561 caaagcgcta cctaggttcc caccattcaa tacggctttg atttcgggac gggagtttcg
     4621 ctgttcattc aatgtttcat tgtttgtgtc aaataagatc tttccagtag catccattaa
     4681 agtaatccgt tcgttcgact gatggacgaa ttcttccaaa gcagttttgt tttgcgctgc
     4741 ttggaggttt tcgacatcta attgccggat cagcagttgt gctttttttg tcaggtagct
     4801 ttcttgctgt tccacgatct gctgttgaaa ataatgggaa atcaattgcc agccaccaaa
     4861 aaacaaacta actaaaagaa taagaaagaa gagacgttca taaatttttt tccatttcat
     4921 cgtttgggtt cctgaaactt atagccaaat cctctaatag tcaccaagta ctttgggtgt
     4981 tttggatctc gctcgatttt ttctcgcaaa tgactaacgt gtacatctac gattcggctt
     5041 tgtcctgcga agtcaaaatt ccaaatgcgg tctagcaatg tgtctcggtc gatcacacgg
     5101 tcttttcgtt tcatgaaata aacaagtaat tcaaattctt tcggtgttag ttcaatcaga
     5161 tggtcgtcta cggtcacttg ataattggtc aaatcagcag tgatttgtcc aatcgataga
     5221 tattcaggct cagcttcatc ttttgattgc ttacgaggtt caattcgtct gaatattgct
     5281 ttcatacggg ctaatacttc gcgaggacta aatggtttgg tcaaatagtc gtcagcacct
     5341 atttctaggc caatgatccg atccacttgg tcatctttag ccgtcagcat caaaatcggt
     5401 gtttcatttt tttcttgacg cagccgctga gtgatctcca tgccatccat cgttggaagc
     5461 atcacatcta aaatgatgaa atcaaatgat tgttctaatg ctaattctaa tccttcgcct
     5521 ccatctgtgg cactggttac cttgtagcct tctttttcca aattgaacgt caagagcgtt
     5581 acaatagaag gttcatcatc aacaactagt atttttttca ttcaaatctc ctttaaatag
     5641 gtttcattct gtttatattt tagcatagaa tcagtacaaa gggctatttt acaaaagaaa
     5701 taaatggtat aataggaagg cactttgctg aatagaaagg aagagcagtc aatgattgga
     5761 atcagtgcat gtttaggcgg tctggcctgt cgctatgatg gacagaaaaa agaagtacca
     5821 gaattgatgg aactagttga aactggagaa gctgtcatgg tctgtccaga agtgatcggc
     5881 ggattaccaa ttcctagaga tcccgctgaa atcattggtg gaaatggatt cgatgtgtgg
     5941 aaagatcaag caaaagtatg gacgacaagc ggcgaagatg taacggaagc gtacaaaaat
     6001 ggcgcgatca aagcctatca aaaattgcag gaaaaacaca ttgatctagt catcatgaag
     6061 gaaaaaagcc cttcatgcgg aacccattcg atttatgacg gaagtttttc cggaataaaa
     6121 aaaaatggtg ccggagtggc tactgcttat tttattcagc aaaaaatgac tgtgctctct
     6181 gatgaagaat ggctgaaaac tagaggtgag cagaatggaa atcgaaaaga ccaatcgaat
     6241 gaacgcgctc tttgaatttt attcgacctt attgacagaa aaacaaatga actacatgga
     6301 attatattat gcagatgatt tttcattagg tgagatcgcg gaagaatacg aagtaagccg
     6361 acaagcagta tatgacaata tcaagcggac aagcaagatc ttggaggatt atgaaaaaaa
     6421 actccatctt ttttctaatt atattgttcg agaacaggta ttggaaaata tgactgcata
     6481 tattacagat aagtacccgg aagataaaaa actacttgaa tatgtacagc aaattcaagc
     6541 aatcgaagaa taaataaaag gaatgaagaa aatatggcat ttgaaagctt aactgaacgt
     6601 ctccagcagg cgatgggaaa aataagaaaa aaaggaaaag tatcagaagc tgacgtaaaa
     6661 gaaatgatgc gggaaatccg ccttgcctta ctagaagccg atgttaactt gcaagtagtc
     6721 aaagatttta ctaagcgcgt acgcgaacgt gcggtagggg cagaagtgct ggaaagttta
     6781 tcacctgcac aacaaatcgt aaaaatcgtt gatgaagaat tgacgatcac acttggttct
     6841 gaaacagcag agttaaataa atcaccaaaa ataccaactg ttatcatgat ggctggtctt
     6901 caaggggcag gtaaaacgac ctttactggt aaattagcca attatttaaa gaaaaatgaa
     6961 aatgctcgtc cgctcctaat cgcaggggac gtatatcgac cagctgcgat cgaccaattg
     7021 aaagtattag gtcaacagct agacgttcct gtatttgata tgggaactga agtcagcccg
     7081 gtagagatcg tccgtcaagg gatggagtta gcaaaagaaa agaaaaacga ctatgtattg
     7141 atcgatacgg caggacgtct gcatatcgat gaaacattga tggatgagtt gaaacaaatc
     7201 aaagaattga cgcaaccaaa tgagattttg cttgttgtcg atgccatgac tggtcaagat
     7261 gccgtcaacg tagctgatag cttcaaccag caattaggta ttacaggggt cgtcatcact
     7321 aaacttgatg gggatactcg aggcggggca gcactatcga tccgctcagt gacaggcgca
     7381 ccaatcaaat tcatcggttc tggtgaaaaa ttgactgact tagaagtatt ccacccagat
     7441 cgtatggcta gccgtatcct tggtatgggg gatatgctga cattgattga aaaagcgcag
     7501 caagactacg acgagaaaaa agcggaagaa cttgcacaga aaatgcggga aaattcattc
     7561 gatttcaatg actttattga acagcttgat caagtgatgg gtatgggccc actggaagat
     7621 ctaatcaaaa tgatcccagg aatgaatcaa gtccctggaa tcgaaaacgt caaggtagat
     7681 ccaaaagatg ttgaacgcaa aaaagcgatg gtttattcta tgacgccagc agaaagagca
     7741 aatcctgatc tgttgaaccc aagccgtcgt cgccgaattg ctgccggttc tggaaacagt
     7801 gttgttgaag tgaatcgaat gatcaaacaa ttcaaggaat caagaaaaat gatgcagcaa
     7861 atgtccaaag gggatatgaa catcccaggt atggaccaaa tgtttggttc aggcgtcaaa
     7921 ggaaaactag gcaaaatggc gatgaatcga atgatcaaga aaaataagaa gaaaaagaaa
     7981 aaacggaaat aagatgaaaa aaacagcttc ggtacagact gaaaatgtct actgccggag
     8041 cttttctatc agcaattttt attctttact aatacaatta ctctaaacga agaggtggca
     8101 cagatgagat gtccgtggag tgaacaaaca gaaagtatgc ggcaatatca tgatactgtc
     8161 tggggtgttc ctgaatatga tgaccaaaag ctgttccgaa aattgatgct tgatattaat
     8221 caagccggat tgagctggca gacgatttta aataaaagtg aagcattcga tgaagcatat
     8281 gatcgattcg atatcaaaaa agtcgcaaat tatggagaaa caaaaatcag tgaactaatg
     8341 gaaaacaaag gaattattcg taaccgaaga aaaatcgaag cagccgtcca aaacgccaaa
     8401 gcagtcttga aaattcaaga agaaatcggc agtttttcag agtatctttg gtcgttttct
     8461 aatggaaaaa tcgttgattc ttcttatcat tcacaagaag aagtcccaac aacaagtcct
     8521 ctttcagatg aattagcaaa agatctaaag aaaagaggat ttaaatttat cggaagcaca
     8581 acaatctatg cttttttaga agctgtcggg atcatcaatg atcaccttga cagttgtttc
     8641 cgcaaagaag aggtcgtacg tatcggcaga aagggcgaat aaagcatgcg aaaaatcata
     8701 ttttatggtg ctataagttt agatggctat ttggcaacga aaacggatga tcttcaatgg
     8761 ctttttgaca ctccaacagg taagcaaact acttatgaag ctttttatga aacaatcgat
     8821 acgaccatca tgggcagaaa aacttatcag gaagcaaaaa aatatttgga gaccgaagca
     8881 atttatccag agaaaaagaa tttcgttttt tccacccacg cacaattaca attgccagat
     8941 gctgaggtcg taaacgaaga tcctgttgct tttctaaaaa atctacaaat gacagaagga
     9001 aaagatatct gggttgtggg tggcggtcga ttgatcaaac cgctattgga acatcaaatg
     9061 attgatgaat ggtatatcca gattacccct gtattattag gggacgggat tcctttattc
     9121 caaaaaggaa catatgaagc gcgttttgaa atgctagaca cgacacgatt cggtgaattt
     9181 atcgaattgc attatgtacg aaagtagaag taaataaaat aaaaaaggca actatatgca
     9241 gaaatgtcct gtacatagtt gcctttttta ttaccgttat gctttttact ctgttaattt
     9301 gttagaaaaa tctagatacg catctaaaat cgtttggaag aaacttaatg ttccttcaac
     9361 gatattacca ttttcatcaa ttagattcgc aacgtttccg atatatgctt ccggttgctg
     9421 caaggttggc acattcaaaa agacaagtga ttggcgtaga tggtggtttg cgccaaagcc
     9481 gccgatccca cctggtgatg cagtaacgac taaacctggt ttatgatccc aaacactgtg
     9541 tccataaggt cttgagccta catctaaagc atttttcaat actgctggca cagaacggtt
     9601 gtattcagga gtaacgaaaa cgacaccgtc tagtcccctc acttcctcac gaaaacgagt
     9661 ccattcagca ggagcctgtt ctggagtttc gagatcctca ttgtaaaatg gcagatcatc
     9721 gatttttaca aatacacttt cgtacccttc aggaagcaat ttcgcaaaag cttctgctac
     9781 ttttttgttg taagaatctt ttcttaaact accaataaaa aatccaattt tttttgtcat
     9841 acgatttttc ctccttcaaa ctatctataa taatcttaca aaagataagt aactggagca
     9901 aataaaaaca cttactgaca aaagctacat agaagataga ttacttattt ttaacaaaaa
     9961 agaagtctaa aatcttctat tttctaaaaa aaatcgaatt taatcaagct gtaaagaaaa
    10021 aaagctttac agggtaaaaa aaaactgtta cactacatct tgtgaataaa acaatggagg
    10081 tggaaataaa taatggcagt taaaatccgt ttaaaacgta tgggttctaa aaagagtccg
    10141 ttttaccgta tcgtcgtagc tgattcacgt tctcctcgtg atggacgttt catcgaaact
    10201 gtaggtactt acaatccttt gaaagaccct gcagaagtag ttttaaaaga agacttagtt
    10261 cttgactggt tatcaaaagg tgcacaacct tctgacactg tacgtaacat cctttcaaaa
    10321 gaaggcgtta tgcaaaaaca ccacgaagct aaattctcaa agaaataagg tgaccggata
    10381 tgaaagattt aagcgactta gtcttaacaa tcgttcgtcc gctagtcact tatcctgatc
    10441 aagtcaagtt agacgtcgta gagtcgaaag acttttacga gtacaatcta acagtggctc
    10501 ctgaagatat tggccgtatc attggaaaac aagggcgtgt tgctaaagca atccgcacaa
    10561 tcgtttacgg tgttcggatc aatgcaccga aaaaagtgcg tttgaatatc atcgatgata
    10621 aggaaaagta aataaacgtg ctgtactcga attttcgagt gcagcttttt tgtttttttt
    10681 attaattgaa gaaatgatta tttggcaaaa caagcaagaa agcgtatcct tataggagga
    10741 gggagtgaac agccaatgag aaagaaaatg ttatttattc catttgctct attgttaagc
    10801 gcttgtagtt cggttactca agaatcagac aatacacatc tatcaagcag tatatctgaa
    10861 actgttacca gtccagaaaa aagtagtacc aaaacggctt ccaccactga acaaacaatg
    10921 gctacctcaa ataatgagaa aaagactgct ttagaccagt tgaaagaaca acagccaaat
    10981 gttcccatgc ctctagatgt accagtctct tcaggctatc tcaatatcgc agcgacacat
    11041 acaaagcaag gttattctat tttatattac agaacagatc gtcccctcgg gttgaatgct
    11101 gacgaattga atcaagaaac gcctattgca acctatctat atcaatatgg gtttgcttct
    11161 agtcaagaaa caatccaagt tttgcagcca tttgagatcg acacaaatgg tcagcaagtg
    11221 gacttaggtt ctcgaatcac agggtatcag caaggtgcag caggttcaag ttttcttgaa
    11281 tggcaagaag gaaactggcg tattcgaatc cgtgggaata atatagaagg ccaagatccc
    11341 ttactattag ctaaagaaat cgtagcttat ttggaagaaa acagtctacc agcacctgaa
    11401 caatttggta aaattactgt tgatatgggt gatacgacga atcgagccgt agaagtaagc
    11461 tggcaagaac cgaaaaatgc ctatacgatc actcatcaag atcctatgtc tgcgttgaag
    11521 atggcagttt ctatgaagag gttgtgaaat cagcgcgctg atctgagccc atcatttaac
    11581 tccaagtcac aggaagtaat caaaaatagc acgccgcatt tttgcataat tttggcttaa
    11641 taccttaaaa gaaaactaga attgcctctt cacacatgct aaaataaaac agacaaaaaa
    11701 gaaatgggga tataagttga ctgaatactt gaatgtagga aaaatcgtaa acacacaagg
    11761 tatcaaaggc gaagtccgtg ttatctcaac aacggatttc ccagaagaac gttacaaaaa
    11821 aggaacggtg ctaacactat tccaagacgg aaaagcacct gtggaattaa cagtcaaaag
    11881 tcatcgcaaa cataaaaatt ttgacttatt gagttttgaa ggacatcctt ctatcaatga
    11941 tgtggaaaaa tatcgggatg gcatattaaa agtgtcaaaa gacaactctg tagatctagc
    12001 tgaaaatgaa ttctattacc atgagatcat cggtctgcat gtattagaag atggaactga
    12061 gttaggtaaa gtaaaagaaa tcttgtcacc aggtgccaat gatgtttggg tcgttcaacg
    12121 tccgaaaaaa gctgatatat tgcttccata tatcgcttct gtcgtcaaag aagtcgattt
    12181 agaagctgga gtggtccgtg tagaaattcc agaaggactg attgacgatg aagattgatg
    12241 tattgacttt atttccaaga atgtttgaag gtcctatggg ggaatccatc atcggtaagg
    12301 ctgtagataa aggattatta gatatcaata tctctaactt ccgcgattat tcagataaca
    12361 aacatcagac agtagatgat tatccatacg gcggtggagc aggtatgctg ctcaaagtcc
    12421 agcccatcta tgataacatc caagccattg aaaaagctgc accggatgtc aaaaaaagag
    12481 taattcttct tgacccagct ggtaaacgat tcgatcagaa aatggcagaa gaattttcac
    12541 aagaagaaca cttagtattc atttgtggtc attatgaggg atatgatgaa cgtatccgca
    12601 cattagtgac agatgaagtt tccttaggag attatgtcct tactggggga gaattaggtg
    12661 cgatggtcat gatcgatgca acagttcggc ttttgccaga tgtgttaggg aatcagactt
    12721 cagctcaaac ggattcttat tcaaccggtt tattggaaca tccccaatat actcgcccgg
    12781 cagaatataa agggatgaaa gtaccagaag tattgacaaa tggaaatcat aaattgattg
    12841 aagaatggca attaaaagaa tcactaagaa ggacctatca acgtcggcct gatcttttgg
    12901 aaacacttga atggacacct caaatgacga aatttttaga agaaatcaag aaagaagaac
    12961 aagaaaaaac agcagatgaa gccaagtgac tttttctaaa atcttcttta cagtaggttg
    13021 ataatatgtt agtattttat ggtgagtgca aaagcactta actattacga tattccgctg
    13081 tgagaaaagc tcataagaat atttggaata aggagaagaa aaccatgaat ccattaatcg
    13141 aagaattaac aaaagaacaa ttacgttctg acatcccagc gttccgccct ggtgacactg
    13201 tgcgtgttca cgcaaaagtt gttgaaggta ctcgtgaacg tatccagtta tttgaaggtg
    13261 ttgttatcaa acgccgcggt gctggaatca gcgaaactta tacagtacgt aaagtttcta
    13321 acggagtggg cgttgaacgt acattcccat tgcatacacc acgtgttgca aaaatcgaag
    13381 tagttcgcta cggtaaagta cgtcgtgcaa aattgtacta cctacgtgca ttacacggaa
    13441 aagctgctcg tatcaaagaa atcagaagat aaacacggaa aacttccttg gaaatctgaa
    13501 atttgtgaaa agtccttgta tatcaagggc tttttctttt ttctctatca cagttttaag
    13561 ccatttttgg ctgttttgga ccatttttca atcatattcc cttaaaaatt gtcctagatt
    13621 gtccgacatt gactatcagt gtcgtcaaaa tagggcaaaa tttagtagtc agcttgacga
    13681 caaatataat acgaaaaata ccaaaacatt taccgaattt agcaactttt gaacccaagt
    13741 ttctcaaagg gtcagctatt agcaaaaaag acaaaacatg tacttttcta ctaaaaagtt
    13801 gatgtgatag gcttctatct aaataactat taggagtttt tatcgtgaag caaaatgatg
    13861 gacgtatcat ttggaaaaat caaagtgagt taaaactgat tttaacaatt aatgaattca
    13921 tcgaaaaaca cggaattaca tctagccgtc aataccagaa aaagctatca gaaaatccaa
    13981 attctgcacc aagtatgtgg ttcattaaca aaaaatatgg atcatgggag aatttattga
    14041 ttagtatagg acgagaaaat actgggtatg gaaaatgggc tagaatgtca gaacaagaat
    14101 tattggaaat agtagaagcc tttattaagt gcgagaagat aacctctcaa cgaatgtacg
    14161 aacaaaaatc agtagggaag aatattcctt cacttagtac cattaaaaag atgttaggag
    14221 atatacgtcc actttttaaa gaaaaaaatg atggatctcg atttactgat ttcgaactgc
    14281 tgttagaact aaaaaatgaa ataatccgat taaaactaca ggatgattta tcaatgacaa
    14341 agtttcgaaa actagttcaa tccccaaaac taccatcagt tgatacaatt atgaaaagaa
    14401 cgaacaaaaa ttgggaggaa ttgatggcag agattggatt tgattatcga agaatcaaaa
    14461 tttataagca gagaaacaac ctatccaaga caaagaaaac taaatagtat cactctaaat
    14521 cagacgttca aaattaaaat ttggacgtct tttttattac ccaaaaacga aaggagattt
    14581 attttatgaa aaagccaaga tttaagaatt ggcgtttgct cgcaacactg agtctattat
    14641 gccaaacaat tggtggatcg cttggcccaa cgattgcctt tgctgatgaa attactcatc
    14701 cacaaacggt aactgtggaa ttggatttgg ctcaccagta tgctgtcgaa ggtacattta
    14761 gcgatggtcg tcccatgtca gaagttaccg ttccgcacta tgcagtttac aatggtgtga
    14821 agcaagatat tttctgtatt gagccagggg tgccgattta caatgagttc actcctggtt
    14881 atgagaaaaa tccattgcct gatatgtcgg agaaagcgaa attagtttcc gttttatgga
    14941 aggaagctgg tacggatgtg gatacacata ttgtggccca gaagatgatt tggcaagaag
    15001 ttaatggcta cacgcttcac tcaattaaac gttctgatgg tagtgcagta aatatcgcag
    15061 ccattgagtc aaaaatcaat caagccattg cggattatca aaagaaacca agcttccata
    15121 actccacagt gaaaaccgtg ttaggtcaat cgactactgt gacagatacg aacggcttga
    15181 atttatcgga gtttgatgaa gttgtcgaga atatcgcaaa tattgattat cgtgtgaacg
    15241 gcaatcaact agtgattact ccaaatgcca attcaaaaga gagtggtgaa ttaaccctaa
    15301 agaagtcagc taatacagga acaccggtag cttataaaat ggctggtcaa caaactttga
    15361 tggcgggggc aattgataag cccaatacct acacaatcaa aattgatgtg gaaactgagg
    15421 gttctttgaa gattaaaaaa gtcgataaag aaacaggaaa tgttgtaccg ggaaccgttt
    15481 tccatttaga ctttggaaaa acattacctg caaaagacgt gaccaccgat aaagaaggca
    15541 ttgcgacatt ggatgagatt cctcatggcg cgaaagtaac cattactgaa aaatcagtgc
    15601 cagctcctta tacgattgac actacaccaa tcaccgcaac cattaaatct ggtgagacta
    15661 tttctgtgac ttcaaaaaat actcgcgaaa aaggtcaaat cattttgaat aaaatggggg
    15721 tggaaacagg aactgatctt tggaacgaca aatactcatt agccggaaat acgtttgcca
    15781 ttcgcaaaga cagtccaacc ggtgaaattg tccaagaaat gaccacggat gaaaaaggtc
    15841 atgcggaaac accaaaagag attgccaatg cgttggaatt gggaacctat tatgtgaccg
    15901 aaaccaaagc tagtaatggc tttgtgaata cctttaaacc agtaaaagtc gaattgaact
    15961 atgtcaatca aaccgtggct cttgttacca gtaacgtaaa agggcaaaac caagaaatca
    16021 ctggtgaaac cactttgaca aaagaagaca aagataccgg taatgaaact caaggtaaag
    16081 ctgagtttaa aggagctgaa tatactctct ttactgcaaa agatggtcaa gcggtcaaat
    16141 ggagtgaggc ttttaaacca gagttagtga agggaacgaa agcttctgat gaaacggtga
    16201 ctttgacttt agatgaaaag aatcaagttg ctgttaaaca cttagcgatt aatgagtatt
    16261 actggcaaga aaccaaagca cctgaaggat ataccttgga tgaaacaaag taccctgttt
    16321 ccatcaaaaa ggtggatgat aacgaaaaaa atgccgtgat tacccgagat gttacggcaa
    16381 aagaacaagt cattcgcttt ggctttgatt tctttaaatt tgctggatca gctgctggga
    16441 ctgccgaaac tggcttcaat gatctgacct ttaaagtatc gccattggaa ggaaccagtg
    16501 agattacagg tgccgaagat gaagcaatta cagcttataa cgagcaacta ggttttgatg
    16561 gctatggcaa gtttgaaaat cttccttatg gggattattt acttgaagaa gtagaggctc
    16621 cagaaggatt tcaaaaaatt acgccattag aaatccgttc tacttttaaa gaaaataaag
    16681 cggattacac taagagtgaa tacattttta cgattaccga aaaaggacaa aaacaaccaa
    16741 ttaaaacggt gaccgttcct tacgagaaac tgacgaataa agcattttct gttagcttga
    16801 atcgtttgat gctctatgat ttacctgaag aagaagatag cttaacttct cttgggactt
    16861 ggaaagacgg aggcaaagaa ttgacagacc ttgattctac cgagctagtt gataaattga
    16921 gctataactt gcatgaaatc aaagaagact ggtatgtggt agcgcaagcc attgatgtgg
    16981 aagcgacaaa agctgcccaa gaaaaagatg aaaaagccaa accagtggta attgccgaaa
    17041 caaccgcaac gttggcgaat aaagagaaaa caggaacttg gaaaattttg cataaattaa
    17101 ccgcagaaca agttttggat aaaaccatcg tcttattcaa ctatgtgtat gaaaacaaga
    17161 aaccctatga agcaggtgat gatccagtag cgaaagatgc tagcttgaac aatcaagccc
    17221 aaactgtcaa ttgtacggtg gaacgccatg tttccatcca aacaaaagcc cacctagaag
    17281 atggttcgca aacctttact catggggatg tggtggatat gttcgatgat gtgtcgatta
    17341 cccatgatgt actggatggc tcaaaagaag cttttgaaac aattctgtat gcattacttc
    17401 cggataatac gaccaaagaa atttggaaat ctggcaaaat tgattatgaa gtgaatgaca
    17461 aagaattcac taaaaccgta cttgcggaaa aagtggacac aagtaaatat ccagaaggta
    17521 caagttttac cttcaaagaa attaactatg gcaaagacgg aaacatcaat gggaaacaca
    17581 atgaggatct aaaagaaaaa actcaaactt taacacctaa aaaagttccg acaacaccaa
    17641 gtgcaccaga acaaccggaa ataccaactg ttccaagtga ctctcaagaa tctagtccta
    17701 cagtaaagac attcccacaa acaggggaga aaaattctaa tgttctgctg tttattgggt
    17761 ttaccttgat ctttgcgact gctgggtatt atttctggaa tcgccgaaac taaggtgatg
    17821 tgatgaaaca actaaatagg aaaaaccaac gtgcgcgacc gcctcctaaa aaaagtggtc
    17881 gcgctatttt tgtatccaaa actaaaaatg aaaagaggaa aagaagatgg aattgaaatt
    17941 tgttgtgcca gatatggccg aaacctttgg aaagataagg tatgccggcg aaggcgaagt
    18001 tttaactgaa ggatatgggc ggaatactac agtaattggt cgtagttatc atttgtattc
    18061 cagtaagcaa cgagccgatg atattgaagt ggtggtagct gcggaagccg gcgaaaagga
    18121 ttttgaccaa gatcaaccac taaaagccgt gaatccccat ttggttgcca aaggctatga
    18181 gattgagaat cgtgggttta ccgactacgt gttgtatgtc gatgatttag tgaaagcata
    18241 ggaggaaaag aagatgagat taacagaagg tattgttgta gattcaggac taacgtttgg
    18301 gaaattacga ttttccgcat tacgccgaga agtacggaag caaaatgagg atggaacggt
    18361 tagtaacgag gtaaaagaac ggacttataa tttgaaatcc tctgcgcaag gtcgaatgat
    18421 ccaagttagt atcccagcaa atgtaccctt acgcgagttt gcctatgacg cagaggtaga
    18481 gttggtcaat ccaatcatgg atacggtcgc caattatgtt ttccgagaag gaactactgt
    18541 caattggttc attaaggccg atgatttagt cttgaaacga caaccagatc agggaaattc
    18601 tacaaaccaa aatgaaggaa agaaataggt gaagcgtatg gaaattaatg ttgaacatat
    18661 tttagattgt ctcgatcaat atggaaaagg tgagctaacg gaggaacaac tcactaaagc
    18721 attaacctat gatgaaaaaa tgtttctgat tatgcatcaa gggttactgg aagtaggtaa
    18781 cgatgtcgaa gaagattttg atgtactgaa ttggttaggg gaagaagagt ccttctttat
    18841 ggttatcgaa gtaaatgaaa gtctatgtcg agaagccgaa accgtattgg aagaaattgg
    18901 tgtggaaatg ccggatgcca ttgaagtttt tttaaagcaa ctagtggaaa cgaaacaact
    18961 ccctatagca gtaaatgact agcataactg cttgaaattg gatcaatgaa atcctatgaa
    19021 gcttttttcg attggaggtg gaaaccataa aaatgtacaa agggcatcgt atacgggcag
    19081 gagatcagca cttggtttat cactttgttc ttggctggct actcgctctg ttcatcggtt
    19141 ggatgagcgt cttttatttt caagagctta gacaatttga tatttctaag ctatcacttt
    19201 ctactataga aatcgttcgt tctataaaag atttaatcta tttgctagga agtctcgttc
    19261 tttcaggaag cacgatgttg ctttatatac acttttttca ggatcattgg cgcagtttgt
    19321 ggcatcgaca aaagctagcc cggatgattc tagaaaatca ttggtacgaa gtaaaacaaa
    19381 ctcagagtga aggatttttc aaagacctca atagtagtcg aaccaaagag acaatcagtt
    19441 acttccccaa aatctattac cgtatgaagg atggtttact ttctattcgc gttcaaattt
    19501 cactgggaaa atatcaagat cagcttttaa agctggaaaa gaagttagaa agtggtctat
    19561 attgtgaact agtagaaaag gaactcaaag actcctatgt ggagtacact ttactgtatg
    19621 atataattgc caatcgaatt gggatagacg aagtagtggc agaaagtggg gctttgcgat
    19681 tgatgaaaaa tcaagtatgg gcttatgatt ctttacctca tatgttaatc gctggcggca
    19741 caggtggtgg gaagacttac tttttactca ctatcatcga ggctttatta aaatctgatg
    19801 ccgagctttt tgttcttgat cccaaaaatg cggacttagc cgatttaggt acggtaatgc
    19861 ctcatgtcta ttcccaaaag gaggaaattt ctgcttgtgt ggaagatttc tatgagcgca
    19921 tgatgactcg tagcaaggca atgaaagaaa tgcccaacta caaaacagga gagaattatg
    19981 cgtttcttgg acttccacca aactttttaa tctttgatga atacgtggtt tatatggaaa
    20041 tgttaacgac gaaggaaagt gcggtgattt tgaataagct caaacaaatc gtgatgttag
    20101 gtcgtcaatc tggtttcttt ctgattctag cttgtcaaag accggatgcg aaatatttag
    20161 gagacgggat tcgcgatcaa tttaatttcc gggtggcttt gggtcgtatg agtgaactgg
    20221 gatattccat gatgtttggc gaggttgata aaaaattctt tatgaagcgt atcaaaggtc
    20281 gaggctatgt agatacggga gggagtgtga ttagtgagtt ttatacgcca cttgtaccaa
    20341 aaggatatga tttcttaagg gaaattagcg acattggtgg attaaacgtt aatacggaag
    20401 gataaaataa tagtatgtag cctaggagtg tcaagaatcc tgggcttttt ttacgaggtg
    20461 atttatgaca tttttagatt tattcgctgg gattggtggc ttttgcttag gtatggaaca
    20521 agcgggtcac cagtgtatcg gcttttgtga aattgatgat tttgctcgcc agagttataa
    20581 ggcaatccat gatacaagta aggaggtgga aatgcatgac atcacaagtg tatcagacga
    20641 gtttattcaa tctctcggac cagtggatat cctttgcggt ggatttccgt gccaagcttt
    20701 ttcaattgcg ggaaagcggc aaggattttc cgatactcga ggtactttat tctttgaaat
    20761 cgctcggttc gccgctattc tccaacctaa gtttttattc cttgagaacg tccggggatt
    20821 actcaatcac gaaggagggg ctacgttcga gacgatcctc cgaacgctgg atggattggg
    20881 gtatgatgtg gaatggcaag tgcttaactc aaaggcctac gttccccaaa accgtgagcg
    20941 gatcttcctt atcggacatt ctcgagacgc atgtaccgaa caagtatttc ctatcattgg
    21001 atcgtctcca acatctgatc aaaacatcag aaacttatta aatatcaacc cttccaatcg
    21061 aggaatgggg ggacaagtct atgggtcaga tgacgtagcg cctaccttga ccggtgatga
    21121 aggaatcaaa atcgcattgc cggtagatga tggaatttct gtagcgggta tgttactggg
    21181 aaattttgaa caaggcaatc gggtttatga gataactgga acagcgccta ctttgtcaac
    21241 gaaacaaggc ggaacgaaga tcatgcttcg gaaaaacgaa acagaatatc aagaggtgaa
    21301 gccagggaac agtgtgaacc tggcttttcc taattccaca agtcgtcgag gccgtctggg
    21361 aaaacaaagt gtgcatacct tattaacagg agaccaacaa gcagtcgtca cggatcagta
    21421 tcaaattcga aagttaacac ctagggagtg ctggcggctt cagggctttc cggactgggc
    21481 ttttgatcga gcttctcaag tcaattcaga tagtcagttg tataagcagg ctggaaattc
    21541 tgtaacagtt ccggtcattt ttgatattgc tagaagatta aaggaggtaa aaacagatga
    21601 tttatgatga gttgattggg gaatctactg ggtgattggc aagattcagt cagatccgga
    21661 attggaagaa gaactgtacc ggctcaattt tgatattcgc aaaaacgggg tgaaagtccc
    21721 tgatgatccg tatgtaattg atgaggaaac ggatgctcga atggagctga atcaagtcat
    21781 cagtgaattg gaacgaattg cggactttgc taaagaacct gatatccgcc aatacttgtt
    21841 tgagataaaa gcagagttgg aaattgaggg tattaccgcg gagtaacggc caaataaacc
    21901 actgataggc actgaccgcg ggggatttgc gaagcagaaa tcctccgtgg tgctgtgcct
    21961 gtttatggac ggcgttagcc ggccatgatg tctcaccccc cgtatctaac aggggggtac
    22021 aaaaacacaa aaaataagca agcaacgata aggaatggct gtcctatcaa ggataataag
    22081 caatttgtgt agatgttaac tgatttgaaa aagttaacac cttaaatttg gaggtgagaa
    22141 cttggcacaa aggaatttag attatcggct attaaaagac cggcgtaatg aatatggcgt
    22201 ttcacaaaat aaattagcta cgacttgtgg acttagtcgc ccgtacttaa atcagataga
    22261 aaatggtggc gtaaccgcat cgacaaaaac catgaggaaa atttttgacc aactggaaag
    22321 tttcaatccc gagttacctt taacgttact gtttgactat gtaaggattc gctttcccac
    22381 aacggatgcg aggaaaatca ttcaagagat tctccatttg aaatttgatt atatgctcca
    22441 cgaagattac gccttttact cctatcaaga acaatatgtt atgggagata ttgtggtgat
    22501 gttgtcacat gaagaagata aaggcgtcct tttagaatta aagggtcgtg gttgtcggca
    22561 gtttgaaact tttttactcg cccaaaagcg tagctggtat gactttttcg aagattgtct
    22621 aaaagccggt ggcgtgatga aacgcttaga cctggcaatc aatgatcgag ttggcctgtt
    22681 agatattcct gatttaacga aaaaatgtca gaaggaagaa tgtatctcct tgtttcgtac
    22741 cttcaaaagc tatcgttccg gggaactgtt gaaacctgat gataaagatg gtatgggcaa
    22801 tactttgtat attggcagtc ttaagagtga ggtctatttc tgtttgtacg aaaaagacta
    22861 tgagcaatat atcaagttag ggattccttt agaccagacc gaaacgaaaa atcgttttga
    22921 aatccgactg aaaaatgatc gagcttatca tgcaattcaa gatttgttaa aaggtcgcag
    22981 tattgaaagt accacatttt ccattattaa ccggtacttg cgatttgcgg ataaggtgga
    23041 aggcaaaagg cgaactaact ggcccttgaa tgaacaatgg gggcgcttca ttggacggaa
    23101 tcagaaggaa attcaattaa cttctgaacc gaagccttat accattgaac gaacgttgaa
    23161 ctggcttgga cgacaagtcg cccctacgtg gaagatggcc aaggagttgg atcggttgaa
    23221 tcagacaacg tacattcagg atatggtaaa taatactcga ctatcagacc ggcacaaaaa
    23281 gattttggag caacaaagta tggcaattga aaatttgatt atatgaaaag aggaatcgtg
    23341 atggaaattg tgaaagatat tttattggtg tttggtggca gtatgcttgg tgttactgtg
    23401 atgtgcttga tgcatgcaag tgcagcggcg gaccgagcaa tggaaaagaa aggaaagtga
    23461 tgaaatgaat ttcggtcaaa attt
//
LOCUS       DAYYGO010000036        23358 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000036 DAYYGO010000000
VERSION     DAYYGO010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.036"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            138..995
                     /locus_tag="ACHA5T_001175"
     CDS             138..995
                     /locus_tag="ACHA5T_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFM8886761.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            1110..2030
                     /gene="pstC"
                     /locus_tag="ACHA5T_001176"
     CDS             1110..2030
                     /gene="pstC"
                     /locus_tag="ACHA5T_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFM8886762.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            2030..2914
                     /gene="pstA"
                     /locus_tag="ACHA5T_001177"
     CDS             2030..2914
                     /gene="pstA"
                     /locus_tag="ACHA5T_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFM8886763.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            2929..3735
                     /gene="pstB"
                     /locus_tag="ACHA5T_001178"
     CDS             2929..3735
                     /gene="pstB"
                     /locus_tag="ACHA5T_001178"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFM8886764.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3751..4509
                     /gene="pstB"
                     /locus_tag="ACHA5T_001179"
     CDS             3751..4509
                     /gene="pstB"
                     /locus_tag="ACHA5T_001179"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFM8886765.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGINLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4522..5199
                     /gene="phoU"
                     /locus_tag="ACHA5T_001180"
     CDS             4522..5199
                     /gene="phoU"
                     /locus_tag="ACHA5T_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFM8886766.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            5437..5736
                     /locus_tag="ACHA5T_001181"
     CDS             5437..5736
                     /locus_tag="ACHA5T_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886767.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGRLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKK"
     gene            5777..6469
                     /locus_tag="ACHA5T_001182"
     CDS             5777..6469
                     /locus_tag="ACHA5T_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFM8886768.1"
                     /translation="MLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISIL
                     FVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYL
                     TRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVI
                     GSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPIS
                     VLFFLTVRRLKR"
     gene            6466..7233
                     /locus_tag="ACHA5T_001183"
     CDS             6466..7233
                     /locus_tag="ACHA5T_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFM8886769.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            7333..8901
                     /gene="liaX"
                     /locus_tag="ACHA5T_001184"
     CDS             7333..8901
                     /gene="liaX"
                     /locus_tag="ACHA5T_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFM8886770.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEITQVQL
                     STTTGSIYLKDTDK"
     gene            9198..9518
                     /locus_tag="ACHA5T_001185"
     CDS             9198..9518
                     /locus_tag="ACHA5T_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFM8886771.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            9518..9868
                     /locus_tag="ACHA5T_001186"
     CDS             9518..9868
                     /locus_tag="ACHA5T_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFM8886772.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            10035..10973
                     /gene="hprK"
                     /locus_tag="ACHA5T_001187"
     CDS             10035..10973
                     /gene="hprK"
                     /locus_tag="ACHA5T_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFM8886773.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            10988..11818
                     /gene="lgt"
                     /locus_tag="ACHA5T_001188"
     CDS             10988..11818
                     /gene="lgt"
                     /locus_tag="ACHA5T_001188"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFM8886774.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            11862..12887
                     /locus_tag="ACHA5T_001189"
     CDS             11862..12887
                     /locus_tag="ACHA5T_001189"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFM8886775.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            12902..13840
                     /gene="galU"
                     /locus_tag="ACHA5T_001190"
     CDS             12902..13840
                     /gene="galU"
                     /locus_tag="ACHA5T_001190"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFM8886776.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKKLEAEGSKIDHNPKGITEIKEEKKK"
     gene            13965..14405
                     /locus_tag="ACHA5T_001191"
     CDS             13965..14405
                     /locus_tag="ACHA5T_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFM8886777.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            14408..15010
                     /locus_tag="ACHA5T_001192"
     CDS             14408..15010
                     /locus_tag="ACHA5T_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFM8886778.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDTKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(15078..16184)
                     /locus_tag="ACHA5T_001193"
     CDS             complement(15078..16184)
                     /locus_tag="ACHA5T_001193"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFM8886779.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLASIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            16394..17395
                     /gene="ccpA"
                     /locus_tag="ACHA5T_001194"
     CDS             16394..17395
                     /gene="ccpA"
                     /locus_tag="ACHA5T_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFM8886780.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(17731..20076)
                     /locus_tag="ACHA5T_001195"
     CDS             complement(17731..20076)
                     /locus_tag="ACHA5T_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFM8886781.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            20355..21257
                     /locus_tag="ACHA5T_001196"
     CDS             20355..21257
                     /locus_tag="ACHA5T_001196"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFM8886782.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            21470..23245
                     /locus_tag="ACHA5T_001197"
     CDS             21470..23245
                     /locus_tag="ACHA5T_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFM8886783.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSFYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYSSSKKMLPWFLGLAVSFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEAKKLKERPFLLSKQEWLKLSLFLLPLVGFSIVYQWIEYKNETTYSIAAIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALDASPTFHAVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYQKGT
                     IKKKKEFYLMGSGDGKTMNDLPIVFAFLKSGYAYAWRYKDFHQGGNYSFGTQEQVEQA
                     QELLNLPLMDNWIDSSDPRTPDFQLTKTAKVANVMIKIYQVLAIPVLIASIAGILLMI
                     LGTAFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
BASE COUNT         7443 a         4142 c         4926 g         6847 t
ORIGIN      
        1 tagcatagaa acttttaatg agatagtttt ttttacaatt atgagaattt attttgttat
       61 ttaatgattt tgattcgttt tcatcaagta atgaaaatga taaaataaac atactttagg
      121 attaaaggag atttctaatg aaaaaactta aattattctt accaataata ggattagcca
      181 ttcttttatc aggctgttca aagtggattg atcgaggaga atccattacg gctgttggct
      241 cttctgcttt acagccttta gtagaaacag ttgctgaaac ttaccagaca gaaaatcctg
      301 gaaaattcat caatgtacaa gggggcggaa gcggaactgg tttgtctcaa gtacagtcag
      361 gagcagtcga catcggaaac tctgatttgt ttgcggaaga aaaatcgggg atcgatgcct
      421 cagcattagt cgatcatcga gtagcagttg ttgggatcac tcctattgtg aacaaggata
      481 ttggcgtaaa agacatcagt atggaaaacc tgaaaaaact atttacaggc gagatcaaaa
      541 actggagtga agtcggtgga aaagatcagc cagtagttat attgaaccgc gcttcaggaa
      601 gcggaacccg tagcacgttt gaaaaatggg tattggacgg agaaacagct gttcaggcac
      661 aagaacaaga ttcaagcgga atggtgcgcc agatcgttgc cgacacaccc ggtgcaatca
      721 gctatgttgc tttttcttat gtttccgatg atgtagatac gttgaatgtt aacggtgtag
      781 aaccgacaga tgaaaatgtg acaacgaaca aatggacgat ttgggcatat gagcatatgt
      841 acacaagaga tcatccaaaa aaactgacaa aagaattttt agactatatc ttatctgatg
      901 aagtacaaga taacatcgtt ggagaactag gttatatccc tgtttctcat atgaaagtcg
      961 aacgtgattg gcaaggaaat atcgttaaag agtaatcttt acacaaaatt tacaaactta
     1021 gctaaacttt tacatgttat taaaaccaaa ttgatgttca atttgtacac tgagtgagta
     1081 tgttaaacct gattaagggg gagtcatctg tggaagatgt gaaaaaagta ttattaacaa
     1141 aatcaaaacg ttcaaaaatg gaacagcgtg gaaaattcat cagtttttta tgtatcgcgc
     1201 tgatcgtgtt ggttgtatta tctatttttt attttgtagc tagtaaagga ttggccacct
     1261 tttttactga taagatcaat gtatttgatt ttctgtttgg tacagattgg aatccaagcc
     1321 aaatcggagc aaatgggaaa ccaatggtag gggcattgcc tatgatcaca ggttcgttta
     1381 ttgtgacctt tttatctgct atcgttgcca cgccatttgc cattggtgca gcagtattca
     1441 tgacggagat ctctcctaaa aacggaacga agatcttaca gccggtcatc gagctgcttg
     1501 taggtattcc ttctgtcgtt tatggtttta tcggtttatc agttatcgtg ccttttgtca
     1561 gaagtatttt tggcgggaca gggtttggta tcttagctgg tacatttgtg ttgttcgtta
     1621 tgatcttgcc gactgtcaca acgatgactg ttgatgcatt aaaggcagtg ccacggcact
     1681 atcgggaagc ttcattagcg cttggtgcca caagatggca aacaatctac aaagtcgtgc
     1741 tacgagcagc cgtgcccggg attttaacag cagtcgtttt tggtatggct cgtgcgtttg
     1801 gtgaagcctt agctatccaa atggtcatcg gtaatgcagc attgatgcca acaggactta
     1861 ctactcctgc ttctacattg acgtccatct taacaatggg aatcggaaat acaatcatgg
     1921 gaacagttga aaataatgtg ttatggtcat tagcgctgat cttgctattg atgtcattat
     1981 tctttaatat cgtgatccgt atgattggga agaaaggaga gctgaaataa tgaacgcaaa
     2041 acgctcagat aaaattgcta caggtgtttt atatatagtt tcggggatca tcgtattgat
     2101 tttagcagct ttactacttt atatcttagt tcggggaata ccgcatattt catggtcctt
     2161 cttgaccgaa ccttccaaag cctaccaaga aggcggtgga atcgggatcc agctatttaa
     2221 ctcgatttat ttgcttttga tcacgatgat catcagtttt cctatttctt taggtgcggg
     2281 tatctatctt tcagaatatg ctggaaaaaa ctggctaaca gatgtgattc gcacgtcgat
     2341 tgaaatcttg agctcgttgc cttctgtcgt tgtcggttta ttcggtttct tggttttcgt
     2401 tattcaattc cagtatggtt tctcgattat ttctggtgca ttggctttga ctgtattcaa
     2461 cttgcctctt ttgactagaa cagttgagga gtctttacaa gcggtccatt acacacaacg
     2521 agaagcagga ttggccttag gtttgtcacg ctgggaaaca gtgataaaag tggtggttcc
     2581 agaagcgaca ccagggattt tgactggtgt gatcctaagt tctggaagaa tttttggtga
     2641 agcagctgca ttgatctata cggctggaca aagtgcacct gcgttagatt ttacgaactg
     2701 gaatccatta agtgtttcaa gcccgatcag cattttccgt caagctgaaa cattagctgt
     2761 ccatatttgg aagatcaata cagaaggcac gatgcctgat ggtgtttctg tttcggctgg
     2821 cgcttctgcc gtgttgatca ttgctgtttt actgttcaac ttcggcgctc gtgcgattgg
     2881 caaacgatta taccgaaaaa tgacttcagc ctaaggagaa gaagcaaaat gaaaaaatat
     2941 aactggaatg aaacaaatgt catcaccatg ccaaaagaga aagaagtagc actatatacg
     3001 gatgatctac atgtctggta tggcgacaat gaagcaatca aaggagtgga tctggaattt
     3061 gaaaaaaata agatcactgc actgattggt ccttccggct gcggaaaatc tacgtatctt
     3121 cgttcgttga atcgaatgaa tgatgggatc gctaatacga aaatcactgg gaaaatcatg
     3181 tatcaaggcg tagatattaa tgcgcctcaa gtggatgttt atgagatgcg caaacggatt
     3241 ggcatggttt ttcaacgtcc taatccgttc agcaagtcga tttatgacaa tattacattt
     3301 gctttgaaac agcacggcga aaaaaacaaa aagaaattag atgaaatcgt tgaaacaagc
     3361 ttgaaacaag ctgccttgtg ggaccaagta aaagacagct tggacaaaag tgccttggct
     3421 ttatctggcg ggcaacagca acgtttatgt attgctcgtg cgatcgcgat gaaacctgat
     3481 atcttgctgc tagacgagcc ggcaagtgct ttggacccta tttcgacagg aacggttgaa
     3541 gaaacacttg taaacttgaa agaagactat acgatcatca tcgtgacgca taacatgcaa
     3601 caagcagctc gtatcagcga ctatactgcc tttttctatc taggaaaagt aatggaatat
     3661 gacaaaacta acaagatatt taccagacca aaaattcagg caaccgaaga ttatgtgtcc
     3721 ggtcattttg gttaagacgg aggcctcaac atgacaaaag aaattatttc atctaaagat
     3781 cttcatctgt attatggaaa gaaagaagca ctaaaaggga tcaatctaag tttcaatcaa
     3841 ggggaaatca ctgcgatgat cggtccttct ggttgcggga aatccactta tctgcgttct
     3901 ttgaaccgga tgaatgacct gattccgaat gtaacgatca ccggaagtgt tttatataaa
     3961 gacaaagaca tctatggtcc aaaaacagat gttgttgaac taagaaaaga aatcggcatg
     4021 gtattccagc agccaaaccc atttcctttt tctatctatg aaaatgtcat ctatggattg
     4081 aaattaaaag gggaaaaaga taaaaaagtc cttgatcaag tagttgaaga aagcctgaag
     4141 gcagcgtctg tttgggatga tgtgaaagac aaattgcata aaagtgcttt atctttatcc
     4201 ggtgggcagc agcagcgtgt atgtatcgca cgtgttttgg cagttaatcc ggaaatcatc
     4261 ttattggatg aaccaacaag tgcgcttgat cctgtttcta ctgggaaaat cgaaagtatg
     4321 ctgttagaat taagagagcg gtatacgatg atcatcgtga cccataatat gtctcaagct
     4381 tcacgtattt cagataagac ggcattcttt ttagatggtc acttgatcga atacaacgat
     4441 acgaagaaga tattcatgaa tccagaaaaa caagagacag aagattatat ttcaggtcga
     4501 tttggataag gaggaaacgg aatgctacgt acacaatttg aagaagaatt gctgaatctc
     4561 cataatcagt tctacgaaat ggggatgatg gtgagcagcg ccgtgcacaa gtcagttcgt
     4621 gcatacatca aacacgataa aatcttagcc caagaagtta tcgaaaacga tataaatatc
     4681 aataacatgg aaacacgctt ggagaagaaa agttttgaaa tgatcgcatt gcaacagcca
     4741 gttacaactg atttgcgtat gatcattacg gtcatgaaag ctagctcgga tttagaacgt
     4801 atggccgatc atgcggtgtc tgttgctaaa tccacgattc gcgtaaaagg gcagacgaga
     4861 ataccggaaa tcgaaaaaga gatctccgat atgtccgatt atgtgaaaaa aatggtggac
     4921 aacgtcctag ttgcttatgt caaaacagac gaaaaagacg cacgtacgat cgccaatatg
     4981 gatcaaagag tcaatgacta tttcaatcga atctataatg caacaatcaa aaatatgcag
     5041 gaaaatcctg agacagtcat cagtggaaca gactacttga atgttgccag ttatctagaa
     5101 cggatcggag actatgtgac aaacatctgt gaatggatcg tctacttggc tacagggaaa
     5161 atcactgagt taaatacgaa ccacaatgag attttctaaa ctgaagctgt gattatgccg
     5221 tttgtttagg aaaagaggtt gtgaaatcag cggttgtgaa aaagctgttc tgcatcaagt
     5281 aataagaaca gtcaaacaaa aaatagctcc tcatattttt cgtttggctg gacttattac
     5341 cgcagatgca agcttttgaa caccgtttat tcgggaaagg gtttgtgaca aaacatgtca
     5401 caaacccttt tcatcatttc atagaaagga taaagaatgc aaaaaaaaat tagcatcatc
     5461 actatatttt ttatttgtgt gcttacttca tgggttattc agcatcaagc tgctttatat
     5521 gaagtacctg tcatcgagat cgaaaaagta cgaataaaag aggatcatca attgattaca
     5581 ggaagactga tgaacacgga tcaaaaagga gaatcgattt atttatcggt tccttatcaa
     5641 aaaaatcaaa tagaaacagc aaatataaaa gcgagacaaa tgtatctagt ccaaatgcat
     5701 ggagatgaat ggcagctagt agcaaaaaaa aaatgatggc tggcttttct tcttcacaag
     5761 tatctccatt ggcgccatgt tgcttattgg cggaaaacag ggtctatttg ctttaggaag
     5821 tctcatcatc aatatcgttt tacttttact tttgttgctc atctttacta aaacagaagg
     5881 gatttctcta ttaaatatat ctatcctgtt cgtcgttata gggatgatta ttagcatatt
     5941 ggctttagat ggatggaata gaagcagtat aatcaaaatt tcagctgcag ttacgagtgt
     6001 ttttttagca ttttttattt gtcttttgac aatgaatcat tggaaagacc aaggactgcg
     6061 ctatgaggaa ctgaattatc tgacacgacc gtatcgttct gtttttcttt ctagcgtact
     6121 gattggaaca gctggaggaa cactcgatac cgttatcaca gtcattgcga cattagaaga
     6181 attgactaag cgacaaccag aaataacagc gaaacagtta tggctttcag gaaggcaagt
     6241 gggtcgagat gtgattggct cgatgaccaa tatccttttg ttcgtctata tcagtgggtc
     6301 agttccatcg cttcttttat atttaggaaa tcaatggtcg ttcaaagaaa cgatagaaca
     6361 gcatttatca ttggaatttt tacgcgtcat tgctggcagc ttaggaatag tattatcgat
     6421 ccctatctcg gtcctctttt ttctgactgt aaggaggctg aaacgatgaa tgtacttatt
     6481 atacttgttg ggattttagc aatcagcgtg ctacttattg gcggtacgca aggtatgtat
     6541 attcttttag gcttattcat caatttaggg atctttttcc tgttgttatt cggttatcat
     6601 cagaagtggc cgatactggc attgtcggtc ataggatttt tattgattgc tgtggttatc
     6661 ttatttttta tcaacggtta taatctaaaa atgcgtgcag cttttgtgag tatactgatc
     6721 tttttatttt gtttcctact attgcttcct atcactgatt ttttagcgat ccaaggtttt
     6781 acatcggtag aactagaaga attatctggc ttagataaga ctttagcgat tgattttaga
     6841 ttacttacga gatcgttgct tctgattagt cttagtggtg ctgttttaga tgctagtgta
     6901 gcaatttcta gtgggacatt cgaagtatat caggcaaatc cgcaattatc tttttaccaa
     6961 ttgcgccatt ctagtttcgc tattgctaaa aaaatattgg cgtcaacagt aatgacactt
     7021 ctttttgcat tcatgggaag ttctttagcg ttgatcctct ggtttattga tttagacatt
     7081 ccttttcagc aagtgatcaa tgaaaaatca tttgttctag aatatacgat ggctattctt
     7141 accacattgt ctgcactact tgttcttcct ttaacctgcg tgactacagc atatctcttt
     7201 acaaaaaaga aagaacaact caagatggag taaaagttac aacttaagtc ctatgacatt
     7261 tttactgaat cagtgcataa tatggatgta aggaaaagct tgcaaacaat tttgcttaag
     7321 gaggcaattt ccatgaaaga aagagaacgt attttagatt tagtgaaaaa aggaatcctt
     7381 tcgactgaag aaggcttaga tttattagaa agcatggcaa ctgaaaaaga cgaaaagcaa
     7441 ataaaaaaag aagcagacaa ggtaactgct agtcataaag aaaaagatca ggcaagtcaa
     7501 ttgatcgata aacttgaaaa cggcgaagaa gaaatttctg agccagttga tccaaaggaa
     7561 agagaaaggc aagatcaaga aaacttagaa aagatcttag atgaactagc aactgaagcg
     7621 aataaaactt cggcgcgtct agatgaagta aatgctcaat tcgctgacaa taaggcagca
     7681 cgcaatgaga aacaggaagc attgatgcag ctgaatacaa aagaagaatt aggcgagttg
     7741 acagaagaag aattagcaca gcgtcagacg ttagaagcag aaatcaaaga attagaagct
     7801 agtggcgata cactgttaga agaacaattg aaattagaag cagaattaaa agatatccgt
     7861 aagaatcaat ggtctgagaa aaaagaaacg tttactgata agtttgaatt gccagatgat
     7921 tggaaagatc aagcaacaga tacactgaat caagtaggcg aaaaaatgtc agaagctgga
     7981 tcacagttag gcaaattctt gaagaaaaca ttccaaactg tttcagaaac agtgaatgac
     8041 aacatggaat ggaaagatgt cagcttacgt gtccctggga ttgcgacaac gaaatttgag
     8101 catgaatttt actatgaagc tcctgcagca agtatccttg atatcaaggc agcaaacgga
     8161 aacgtgacgt taaaaacgtg ggacagcaac gatgtgaaag ttgaagctaa gatcaaactt
     8221 tacggaaaaa tgggtgctga accatttgaa gcattctctg aacggagcca gattgaggtt
     8281 aatgaagatc atatctcctt ccaaattcca aataaacgtg tgcgtgcaga ccttgttttc
     8341 tacttaccta aacgagtata cgaccacgca gctattaaac ttctaaatgg caacatcatg
     8401 attgaaacat tggaagctaa agatatttat acaaaatcta cgaatggtaa catcatcgtg
     8461 gaccaattaa ctgcaacgat gctggaagta gaaggtgtaa acggcaatat cgatattcga
     8521 aacgggaaca tcttagattc tattatcgaa acagtaaatg gtacagtgac atttggtgcg
     8581 acagcagaaa acctttctgt gtctttggta aatggtgatg tacgtcttac gattaaagaa
     8641 gataatttga aaaaagtaga agcaagttca gtgaacggaa atgtcaaagt cgctttacca
     8701 gatggcatcg gtttggaagg acatgctaaa acaagtcttg gaagtatcaa cagccgtctg
     8761 tccaattacg aagtagttcg tgagaaaaaa gaacggacaa accaaatgtt gcaattccgt
     8821 cgtgtttcag atggtgaaat cacgcaagtg caactttcaa cgacaacagg aagtatctat
     8881 ttaaaagata cagataaata aagaggttgt gaaagagatg ttcagccttg agcaataaga
     8941 agcgattttg taaaatagct tcccctattt tacaaaattg tgccttattg ccgataggct
     9001 atctcttgaa caccgtttac caagagatta tgaacctgcc aattatcgcc gagtcacaag
     9061 gaataatttc agaaaaagct cgtcacgttt tatgaatatg gtaatttgtg accgagaatt
     9121 ttgcagaaag acaccgatta agaagaaggt tgtcacaacg attttcttca tttaaaatag
     9181 tagaacagag gtgagacatg atgaaaaaaa agttgacaaa atccaataaa aatgttgttt
     9241 tgacagggac tttagcagga ataggagaat acatcgggat tgatccgact gttatccgtg
     9301 tgctttatgt attcctgagc ttagttgctt ttggaagtcc aatcatttta tacattttat
     9361 tagctttgat tatcccatct gcgcgttctg cacgaacaaa ctatggacat aacaatgcat
     9421 attatgaaaa aaacaattat cgtgaacaaa ctcgtgaaca aacaaaaaaa cgcaaagaag
     9481 cggaaaaaat cgatgatgac gaatggagtg acttttaatg tcgtactttc aacgtatttt
     9541 agttaatacg ttaacattca tatcattggc tgttcttttt cctgcgatgg tccatgtgag
     9601 cagtatttgg acggccgttc ttgccagctt cgtattgtct gtgttgaata tgtttgtcaa
     9661 accgattttg acgatactgt ccctgccctt tacattagtc acattgggat tgtttacgtt
     9721 tgttatcaat gcagcgattc ttaaaatgac ctcctttttt gtgggagcag aacattttag
     9781 tttttcttca ttcggtgcag cgttgttagt agctatcttg atgtcgcttg tcaatgtaat
     9841 tgtcagtgaa cataacagta gaaaataaaa gaaagctgtg ccgaactatc ggcgcagttt
     9901 ttttatttag aatcaggaat gcgttttcta ttacattggt aattaaataa aaacgttttt
     9961 tttaattggt agtacttttg gctagacaat gataaaatga tagagaacac tctaatagaa
    10021 atgaggtagc atgaatgtca gaagttgtaa aagttagcca attagtgaaa aaattatctt
    10081 tggaaatcgt gacaggcgac gagaaaagct tagaccgtgt catcgtaact ggggacatct
    10141 cacgcccagg actagagttg actggttatt ttaattatta ttcccataat cgtttacagc
    10201 tatttggcag taaagaaatc agttttgcgg aacggatgat cccagaagag cgtttgatca
    10261 tcatgagaag attatgtagt gaagatatgc ccgcatttat tatttcaaga ggattaccgg
    10321 ctccagaaga aatgatccag gcagcaaatg aaaatgggat agctgtactg cgttctccca
    10381 tctcaacttc tcgtttatta ggagaattat ccagttattt agatggtcgc cttgcccctc
    10441 gaacaagtgt ccacggtgtt ttagtggatg tttacggatt aggtgtactg attcaaggtg
    10501 acagtgggat aggaaaaagt gaaacagcgt tggagttgat caaacgcggt catcgattga
    10561 tcgcagacga tcgagtagat gtttatcagc aagatgaatt aacggtgatc ggagaaccgc
    10621 ctaaaatcct ggaacatcta attgaaatcc gaggaatcgg gatcattgat gtaatgaatc
    10681 tttttggtgc gagtgcggta cgtggctcta tgcaagtcca gttggctgtt tatctggaag
    10741 catgggcaaa agataagaaa tacgaccgtc tgggctctga agacacgacc gttgaaatcg
    10801 cggaagtggg cattccacaa ataaaaatcc ctgtcaaaac aggacgaaac gttgcaatca
    10861 tcattgaagt agccgcgatg aatttccgtg cacgtacaat gggctttgac gcaacgaaaa
    10921 catttgaaga aagattaagt cgcttgattg aagaaaactc aggcaacgac tagcaaggag
    10981 tgacaatatg ttagcacaaa tcaatcgagt tgcttttgag cttgtaggga ttcctatcta
    11041 ctggtatgca ttgatcatcg tatcaggtat cattatcgcc atgtggctta gcagcagaga
    11101 ggctgttcgg gtaggattaa aagcagatga tgtgacagat tttatgttgt ggggattgcc
    11161 tatctccatt attggtgctc gcttatatta tgttttattt gatcttcccc agtatatagc
    11221 tgatccaatc caaatattca atatccgttc aggaggctta gctatttatg gcggactgat
    11281 tgcaggcgcg atcacgctgt atttttttac taaatatcat tttatttctt tgtggacttt
    11341 tttagatatc gctgcaccaa gtgtattatt agcacaggca atcggtcgtt ggggaaattt
    11401 catgaaccac gaagcatatg gaccagacac gacgcgaagc ttcttagaaa gtctgcattt
    11461 accgcaattc atcattgata atatgtatat cgaagggatc tatcgtcaac caacatttct
    11521 ttatgaatca ctttggagtt tgattggatt tattttgctt ttgtttttac gaaagaaacc
    11581 acaccttttg aaaaaaggcg aagtcctttt atgttatcta atgtggtata gtttcgggcg
    11641 tttctttatt gaaggattac gtacagacag tctttacttt ttaggtgtga tccgtatttc
    11701 tcaattatta tctgctgtgt tatttgtcgg tacattgatt ctatttattt ggcgcagaaa
    11761 aaaaggaaat ttaccggatt atgaccgctc attaggaaaa aatcaacaaa ttatctgatt
    11821 tttagagcgt taaaaatgaa aaaaatttca ggagtggtaa aatgaattat agagtagcgg
    11881 ttttaggacc aggctcttgg ggtactgctt tggcacaagt cctcgccgaa aacggacatg
    11941 acgttcggat ttggggacat cgtgcttcac aagtagaaga aatcaacacc caacacacga
    12001 atcaacgtta tttaccagat cattatctac ccgattccat ccaagcattc accaatatgg
    12061 aagatgcagt aaaagacgca gatgcggtgc tattcgtcat tccaacgaaa gcaatacgtt
    12121 ctgttgcaaa agaactagtg cctaaattga atacaaagcc agtgatcatc catgcaagta
    12181 aaggattaga acagggaaca cataaaagga tctctgacat cttgttagag gaaatcccag
    12241 cagaaaaaag aaaagctgtt gttgttcttt ctggaccaag ccatgcagaa gaagtagcga
    12301 aacatgacat cactacgatc acagctgcaa gtacagatgc aaaagcagca aaatatgtac
    12361 aagagctgtt catgaataat tattttcgga tctataccaa tcctgatgtg atcggtgtag
    12421 agatgggggc agctttgaaa aatattattg ctattggtgc tggcgctatt cacggcttag
    12481 ggtttggcga taatgcaaag gcagcaatca tgacgcgcgg attagctgag atcagccgtt
    12541 taggtgtagc aatgggtgcc aacccgctga cctttatcgg attaagcgga gtaggcgatc
    12601 tagtcgttac ttgcacaagt gttcattcta ggaactggcg cgcaggcaac ttgttaggac
    12661 aagggcagcc cctagaagag gtgctagcca atatgggaat ggtcgttgaa ggtgtgaata
    12721 ctactcaagc agcgatggaa ctagcccaaa taatggaaat cgaaatgccc atcactcaaa
    12781 caatctatga agtgctgtat gaacaaaaag aaatcaaaac agccgcaaaa gaaatcatgt
    12841 tacgtgatgg gaaaaaagaa aatgaattct cattaacaat tttttgaagg agcgaatgta
    12901 tatgaaagtc aaaaaagcag ttattccagc ggcaggatta ggaacaagat ttttaccagc
    12961 aacaaaagct atggcaaagg aaatgttgcc aattgttgat aaaccaacaa tccagttcat
    13021 cgttgaagaa gcgttgaaat caggaatcga agacatcttg atcgttacag gaaaagctaa
    13081 acgaccaatc gaagatcact ttgattcaaa tattgaatta gaaacaaacc taagtgaaaa
    13141 aggaaagacc gaattattga aattagtaga agaaacgaca gatgtcaact tgcatttcat
    13201 ccgtcaatct catccaaagg gtttaggaca tgccgttctt caagcaagag cctttattgg
    13261 aaatgaaccg tttgtcgtta tgctaggcga tgatatcatg gaagatgaag taccattgac
    13321 acagcaatta atcgatgatt acgaacgtac tcatgcttct acgatcgccg ttatgcaagt
    13381 tcctcatgat gaaacttcaa aatacggaat tatcaatcca ggagaagtat tggaagatgg
    13441 gttatacaat gtcaaaaact ttgttgaaaa accagatcca agcaaagcac caagtgattt
    13501 agctattatt ggacgttact tattgactcc agaaattttt gatgtattgt ccgagcaaca
    13561 accaggagcc ggaaatgaaa tccaactaac tgacgcaatc gatacattga ataaaacaca
    13621 acgcgtattt gcccgtaaat tcacaggtaa acgttacgat gtcggtgaca aatttggttt
    13681 tatgaaaaca agtattgaat atggtctaac acatccagaa gtaggtgcac ctttacgtga
    13741 gtatatcctg tctttaggta aaaaattaga agcagaagga agcaagatcg atcataatcc
    13801 aaaaggaata acagaaatca aagaagaaaa gaaaaaataa cccttttttc taacgagctt
    13861 cagctgctaa ttgaataaaa agtagttgaa gctcgttttt atttgtttaa aaaacacgaa
    13921 aatgctatcc ttatagaaaa gagagacaag aagggagttt gatcatgtca ggaggagaaa
    13981 ttgcaggatt gatcgcagca atcgcctttg cgatacttgt cgtttttatc gttcgtgtgt
    14041 tgttgcaagt ttctaaaact ttagaaaaag tagatcagac tgtagcagaa gcaaatacga
    14101 cgatcgaaat cgtcacaaaa gacgtagatc ttttgtctag acaagtagaa ggcttgctgg
    14161 tcaaaagtaa tgaactgctc ttggatatca atggaaaagt cgcaacgatc gatccattgt
    14221 tcacagcagt tgctgattta agtgaaagtg tatcagaact taatcattct agtaaaaaca
    14281 tcgcgacgaa agtcggcgga atcggtaaaa cgacagctaa agcgacggtt gccggaaaag
    14341 tcggtgggac agcgatgaaa ttttttaaaa acaaaaaaca tactgaaacg acaggaggaa
    14401 aataaaaatg gctaaaaaag gtggattttt attaggtgca gtagtcggag gaacagccgc
    14461 agcaatcgcc gcgttattgt tagcaccaaa atctggtaaa gaacttcggg atgaattagc
    14521 tgcccaagcg gatgatttga tggatatggc ttcagattac acagatatgg cgaaagaaaa
    14581 atcgactgag ttaacaggta ttgcaaaaga aaaagcgcaa gatctttctc aacaagcagg
    14641 tgatctagca ggtagtgtca aacaaaaagt aagtgaatca gcagatgaag caggaagtga
    14701 atctgacgat atcttgttaa atcttaaaga acaatcagct gacttatcta atcgtttcaa
    14761 aaaaacggca gaagatatga aaaaacctgc agatgaatta ggaagtattg ctaaagatac
    14821 ttcagaagat atcatgattg atgtaaaaga cgctgctggt aaagcaggag atactgtcaa
    14881 tgaaggaatg tcagaagcaa aaccagttgc agatgacaca aaagaaatga tgaaaatggc
    14941 aaaagaagat gcgaaagaag ctaaaaaaga aataaaagat acaatgacag attcatctac
    15001 aaatcactaa aatcactaaa atcacaaatt tatagaataa aaaggtggga gcggactagc
    15061 tctcaccttt tttactctta tttttctaaa actagtaatt cttttggtgt agtcgtgaat
    15121 ggcacacagc catcgcttgt cacatataca cagtcttcga tacgtacccc tactttttca
    15181 ggaatataga ttcctggttc aagagagaag cacatccctt cttcgattac taagtcattt
    15241 ccttctacaa gggaagggaa ttcgtggacg gttgttccga tcccgtgacc taaacggtga
    15301 ttgaaatatt cgccgtagcc atagctgttg atcacgtttc gtgcgatttg atcaagttca
    15361 cctgctgtca cacctggacg gacggcttct tgtgcggcaa gctgtgcttc aagagtgatg
    15421 ttgtatattt tttcttggaa gtcggttggt ttttgataag caattgtacg agtagcatca
    15481 ctgcaatagc cattccaaac aacgccaaga tcgaataaca caagttcatt cggtgaaatc
    15541 ttcgtgtttc ctggtgtacc atgagggctt gcagcatttg ctcctgcaag gactaatgtg
    15601 tcgaaagaca tttgtgaaac accttgtttt ttcaattggt attcgatttc tgcaatgatc
    15661 gcttgttcag tcacgccttc tttcactgct ttaaagccaa tctcaaatgc aacatcggcc
    15721 catttaccag cttcgatcag tttgtctttt tcctctagag ttttgatcag ttgtagacgt
    15781 tggatgatgg aagctagatc agtgtaagaa tccgttcccg tcaagatatc agagatagca
    15841 tcgaagcgct cgacagtcaa ggcgttcttt tcaatcccta cattgccagg ctgaccatag
    15901 cgagcacgaa gttcttttcc gatgatctcc caagggtttt cgctgtctaa atagccacgg
    15961 acatcatacg cccaactact gttttgagca tcttcaactt ccaaagcagg agtgaaaagg
    16021 aaaggatctt gttcaagagg gataaaaaga gctagtacac gctcatgagg atcactttcg
    16081 tatccggaaa agtaagcgat gtggccaggg tcgctgatat aagccaattc gacttggtgt
    16141 tgtgccatcc attgacgtag ttcattgatt tttacgttat tcatttggca acctactttc
    16201 taaattaatt ttgtattcaa taattgtacc actgatttat taaaagacca acgttctaaa
    16261 aactgaaaaa agtaaaaatt ttcatgaaaa cagagataaa acggttgcaa ttcttgagat
    16321 tatttgatat tctattttag aaatgtaaga cgtattttca gaaaacatat aaaatatgaa
    16381 taggagaaca caaatggaaa agcaaacaat cactatctat gatgtagcga gagaggcaaa
    16441 tgtttcgatg gctactgttt cacgtgtcgt aaatggcaat ccaaacgtca aaccagcaac
    16501 aagaaaaaaa gtcttggaag ttatcgatcg cttggattat cgtccaaacg cagttgcgcg
    16561 tggcttggca agtaagaaaa caacaacagt tggggtaatc atccctgatg tcagcaatat
    16621 gttctttgct tctttagcac gtggtatcga tgatgtggca acaatgtaca aatacaatat
    16681 tatcttagca aattcagatg gtaacgacca aaaagaagtc aatgtattga ataacttact
    16741 ggctaagcaa gtggacggcg tgatcttcat gggtcaccat atcacagacg aaatccgtgg
    16801 agaattttca cgttcaaaaa ctcctgttgt attagctggc tctattgatc cagatgaaca
    16861 agtaggtagc gtaaacattg attatgtatc agcaacaaaa gatgcagtaa acaaattggc
    16921 aaaaagcggc aataaaaaaa tcgcttttgt cagtggtgcg ttgatcgatc ctatcaatgg
    16981 acaaaatcgt ctgaaaggct ataaagaagc attaagcgaa aataacctac catatagcga
    17041 aggattgatc tttgaagcac aatacaactt caaagatggt ctatctctag tcgatcggat
    17101 ccataatagc ggtgcaacag ctgtctatgt ttcagatgat gaattagcca tcggtatttt
    17161 ggatggttta ttagaccgtg gcgtgaaagt accggaagaa ttcgaaatca tcacaagtaa
    17221 caattcacta ctgacagaag tagctcgtcc tcgtttaaca agtatcacac aaccattata
    17281 cgatttaggg gcggtatcta tgcgcctatt gacgaaaatg atgaataaag aagaagtcga
    17341 agaaaaaacg attatcttgc cttatggaat cgaagaaaaa ggatctacaa aataataaaa
    17401 ggacaaccat tcagaggttg tgagcctgcc gtttaactct gcgttacaag gagcaatttc
    17461 aaaaaacagc atttcatgtt tgatgaatat tgtgactcgt gattcggagt tggcaggaga
    17521 acaccgttta ttcgggaaaa ggggttatga caaaattttg tttaatcctt tttttttgtc
    17581 ttaaaaaaga gagaaagtgg ttgaaattac atttatcgta tataaaacaa tccgtgtata
    17641 gatgctgcat tatgttcacc accatcaaaa aaataaggga ctgtgacaat actattgtca
    17701 cagtccctta ttactgtgta ttctattaaa tcaatcatct ttctcatctg attctttggt
    17761 tgcttgattc gctcttgcat aagctgaagc ggtgttccag taatctgtat agttttcatc
    17821 ggtaccgcca acgccaaatt taaaggcgct tttcttcggt ccatttttcg cccatagcga
    17881 ttcaactgtt tttcctggtg tttggatgga ttttttattg acagtaactt ttccaggtgt
    17941 ttgaccagta aatccagata cttttttctt catcacatca gatgataatt cgaatttctc
    18001 atcagtgcca aaaatgtcag gttttgcttg gtaagcttga ttgatcagat tggcaagata
    18061 attagccgaa cgtatccttg ctttgctgtc cattccagta ttgtcatcgt gccctgccca
    18121 gctactgatg gtaatagacg gagtagagac aattagccat gaatctcggt attcattggt
    18181 agaaccagtt tttccgaccc aatctgcttt tcctagattt ccgtttaagc ttgagatggc
    18241 atctttgaat ggtgtagtga tttttgcatt gatcacactg cgcatcatgt cattcatgat
    18301 cgatgccgtt gcttccgaat agatccggac agagttgctt tcgtgtttat aaattacatt
    18361 tcctgcgtta tctgtgatgg aatcgatgat atagccttct tcatagacgc cgccgtttgc
    18421 taaagcctga aatccattgg tctgttgaag cgtcgtgaca tctactgtac ctaaaggagc
    18481 agattcaacg ccccaattgt catttgccgg gtagttcatc tttgccaagt agtgctcgta
    18541 agcatattgt ttcgatccac cggaattcaa cacatcttga tataagtgat aagccgggat
    18601 attgttggac cattctagtg actcacggac ggtttggaag gtgttcgatc cttcgttagt
    18661 agcattgacg attttctcgc cagcattttc tccttcttgc caagttgttg cataatctga
    18721 tacacgggat tctgatccga taagccccat gtcgattgca ggtccataaa caagaactgg
    18781 tttgattgat gagccagctt gacgttctgt atcgaaggca tggttatttt gattctgact
    18841 gtaattccgt ccgccaacga atccgtagat gcggccagtt ctgttatcca tcaatacatt
    18901 tccggtttct acttgactgc cagcaccatc atctaacatg tatccataat tttctacgcc
    18961 attttgcaat gcagcataga catctttatc gattgtcgaa tggattgtat atcctttatt
    19021 ttggatcgtt tgctgtgcct tttgataata agcgtcactg accgatgaat cagacatatc
    19081 agcgttgtct ttttcagcca attgttgtgc aatgattctt gttgcttcat tcatcacagt
    19141 ataatacaag aattctctgt cagtctgttc tgcgatttgt tgtggtagaa agtcttttgt
    19201 aaggtcatag gcttttgctt cttcgtattg ttctttcgta atttggcctt cacgatacat
    19261 actgaataaa acaaaatctt ttctggcaag gccggcagaa agatcttctt ttagcgcacc
    19321 tgtgttcgtg tatggactat acgtgatcgg gctttggggt aatccagcga tataagcagc
    19381 ttgcggcagc gtcacatctt ttgcatctac accaaagatt cctcttgcag cttcctggac
    19441 tccagcaatg ttttgtccct tattattacg accaaatggt gagacattca aatacgtagc
    19501 aatgatctca tcttttgaaa agtatttttc tacttgtgca gaaagaatga tctcgtttgc
    19561 tttacgtttg aatgttgttt catctgtaag gatctgctgt ttgactaatt gctgtgtcaa
    19621 agttgatcca ccagaagagc caataccagt agcttcagat actaatgctc gtaaaacagc
    19681 tttaggaacg taaccttggt gtttatcgaa gtattcatcc tctgtgctga taattgcagt
    19741 tttcaataaa ggtgaaattt tatcagatga aatagtcgtg cgcatcagat ctgtctggat
    19801 ttttgatatc tctgtattat ctgcataaac aatctttgac gtttctgtaa tgtttcctag
    19861 ttctgtctgc agtgcttttt tagtcggcaa ttgtgtgtct tcgacgagat aggcaaaata
    19921 gccagcgcct attccaatac ctagagctgc gaacatcaaa agaagaacag taataaaaac
    19981 agtcaatgat tgaaaaactc taaaaataat cttaggaaca aaccattttt ctttttttct
    20041 tctattctgt ttttttcgag tatttttgcg tggcaaaagg gtcacctcaa ctaatggttt
    20101 tatttcgcta attatagcaa ataatagaac aaaaaaacag acaaggctgt ttttggcagg
    20161 aatttctttt ttaaaagagt taaaacgtga gaaaatgatc ttttttataa aagagttctt
    20221 aagcttttct ttagagaaaa tttcagttac tatggagaac agggcaaaga aaaatcaatc
    20281 attttctttg tatatcatct taccaatata taggaattgt tagtaacaat tgcctttcaa
    20341 ggaggaatcc atccatgtta ttaggctcac acgtcagcat gagcggaaag aaaatgctgt
    20401 taggagcagc agaagaagca gccagttatg gatcttcgac ttttatgatc tatacaggcg
    20461 cccctcaaaa cactagaaga aaagccatcg aagagatgaa tattgatgcc ggaaaagcgt
    20521 acatggcaga aaacggtcta acaaatatcg tcgttcatgc cccttatatc attaatttgg
    20581 ggaatacgat caaaccagaa atgtttcctt ttgccgtcca atttctgaga gaagagattg
    20641 ttcgtgcgga agctttaggc gctactcaaa ttacacttca cccaggtgct catgtagggg
    20701 caggaacaga agccggaatt gctagaatta ttgaaggctt gaatgaagtt ttgacaaaag
    20761 aacagacggc acaaatcgct ttggagacaa tggcaggtaa aggtacggaa atcggtcgaa
    20821 catttgagga attagcgcaa atcatcgatg gagtaaaatt gaatgaaaaa ttatccgtta
    20881 cttttgacac ctgtcatacg aatgatgccg gttataatat taaagaagac ttttcaggtg
    20941 taatggaaga gtttgataag atcatcgggt tagatcggct taaagtactc catatcaatg
    21001 attctaaaaa tccgcaaggt tcgcataaag accggcacgc caatattggt tttggaacaa
    21061 tcggattcga cacgctgaat aagatcgtcc atgatccaat gtttgacaat atttcgaaaa
    21121 tcttggaaac tccctatgtc ggcaaagata aaaagaaagc ttacgcacca tatggaaaag
    21181 aaatcgccat gttcaaagca caaatattcg atccagaagt gtttcctgag ttattagcag
    21241 aagaagtgat actctaagat tttctatctg acagcagcaa aaaaggacca ggcacattgc
    21301 cttgtccttt tttgtttaag aaacaaagga gtgctttttg cagtcttttt catcatgcat
    21361 cttctccccc ctctttttaa atttagacaa acagggtata ctggtagaaa agtaaaaaaa
    21421 cgaaaagttt cacaagcaag tggcaaaacg tgattggaag gacgaaaaga tgctgaacac
    21481 tttaaaaccc ttgatcaacc aactaaaaaa gaaacataca tactttattc ttggcatagc
    21541 cctattattt acaattattc gtttgtattt atatagtaaa gcaatctatg gaacagacat
    21601 gtcggcaacc tatgatgacc agctgctgat tcgctatgct caaaatattt tggacggtaa
    21661 atggctaggg gagtatacga ctacaacact ttccaaagga atctcctaca gcttattttt
    21721 agtacttgca cataaactgc atctttctta tgcagttttg ttcggcatgt tcagtatttt
    21781 tgccagtata ttggttgcac tcgcgtttaa accgatcgtg aagaacaagt atcttctttt
    21841 aagtttctat ttttattttt tgttttctcc gattagtttt acacatgaat attccactcg
    21901 aacgtatcgt aatgccctcg tgattccggc tgttgagatc attatcgctt gtttactagc
    21961 tatttattac cgtaaatata gttccagcaa aaaaatgctg ccgtggtttt tagggctagc
    22021 tgtttctttt cctttctttt ggttcattcg cgaagattcc atctggcttc ttccttttac
    22081 tgtagtcgcg ttaggcatca gtttcgtcca gatcttgcta ggacaagact tttctttcaa
    22141 agaagcaaag aagttaaaag aacgaccatt tctcctttcg aaacaagaat ggctgaaact
    22201 ttcattgttt ttactccctc tagtcggttt ctccatcgtc tatcagtgga tcgaatataa
    22261 aaacgagaca acatatagta ttgccgccat caatgaccgt tcgagtggcg aatttggacg
    22321 tttgatgaaa cagctgatcc gaatagatga cgaaacgaac ttaaataaag aaaacagtga
    22381 gatatgggtc tcgcatgctg cattaaataa agcattagac gcatctccta catttcatgc
    22441 tgtttctact cgtattgacg aattgtatac gactcatgcg tggacccaag gtggaaaagt
    22501 aaaagaatta ccaggagata tcattttttg ggcattgcga gatgtggtaa gcgaatctgg
    22561 ttattaccaa aacaatgcaa aagaaaccaa tgatttttgg aaaaaagtca atcaagaatt
    22621 agaagaagcc tatcaaaagg gaactatcaa aaagaaaaaa gaattctatc tgatgggatc
    22681 aggggatgga aagacgatga atgatctgcc aatcgtattt gcttttctga aatcaggata
    22741 tgcctatgca tggcgataca aagatttcca tcaagggggg aattatagtt tcggtacgca
    22801 agaacaagtc gaacaggccc aagaattatt gaatcttcca ctaatggata attggatcga
    22861 tagcagtgat ccgagaacac ctgatttcca gttgacaaaa acagcaaaag tcgcgaatgt
    22921 catgataaaa atttaccaag ttctggccat tcccgtgctg attgcttcaa tagcagggat
    22981 attacttatg atactaggaa ccgcatttgc aaaagctgat cgtgcctatt atagttcctc
    23041 tttgattgta gtcaccggat taatactgac acaattggtt tttcttttcg gcgtctcgtg
    23101 gttttgttct tatgcgccag aaaaaaagga ttatttttta agtgtctaca ctggcgcagg
    23161 cgtgccatta acgcaattca tcgtagtaac aagcatgcta gttttcagta aattgaatct
    23221 gaaaaaagct ttatcttcca aataaaaaca tactaagatt agaggagaac atcattgacg
    23281 aatgatgttc tcctctattt tctattttta gattatagtg aagagtgaaa gaaagttcga
    23341 acaaagcctt gaggacta
//
LOCUS       DAYYGO010000037        23154 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000037 DAYYGO010000000
VERSION     DAYYGO010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.037"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..583
                     /locus_tag="ACHA5T_001198"
     CDS             <1..583
                     /locus_tag="ACHA5T_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM8886784.1"
                     /translation="TMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGGEAVNWY
                     NEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQIKHVTIP
                     QLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTSTGDFGM
                     TAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            595..1515
                     /locus_tag="ACHA5T_001199"
     CDS             595..1515
                     /locus_tag="ACHA5T_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8886785.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            1556..3013
                     /locus_tag="ACHA5T_001200"
     CDS             1556..3013
                     /locus_tag="ACHA5T_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8886786.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3358..3972
                     /locus_tag="ACHA5T_001201"
     CDS             3358..3972
                     /locus_tag="ACHA5T_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM8886787.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            3965..5689
                     /locus_tag="ACHA5T_001202"
     CDS             3965..5689
                     /locus_tag="ACHA5T_001202"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8886788.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            5686..7137
                     /locus_tag="ACHA5T_001203"
     CDS             5686..7137
                     /locus_tag="ACHA5T_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8886789.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(7609..9762)
                     /locus_tag="ACHA5T_001204"
     CDS             complement(7609..9762)
                     /locus_tag="ACHA5T_001204"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFM8886790.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(9764..10810)
                     /locus_tag="ACHA5T_001205"
     CDS             complement(9764..10810)
                     /locus_tag="ACHA5T_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8886791.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            10960..12249
                     /locus_tag="ACHA5T_001206"
     CDS             10960..12249
                     /locus_tag="ACHA5T_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFM8886792.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            12262..14964
                     /locus_tag="ACHA5T_001207"
     CDS             12262..14964
                     /locus_tag="ACHA5T_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFM8886793.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            14949..15806
                     /locus_tag="ACHA5T_001208"
     CDS             14949..15806
                     /locus_tag="ACHA5T_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8886794.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(15877..16734)
                     /locus_tag="ACHA5T_001209"
     CDS             complement(15877..16734)
                     /locus_tag="ACHA5T_001209"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM8886795.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            16945..19116
                     /locus_tag="ACHA5T_001210"
     CDS             16945..19116
                     /locus_tag="ACHA5T_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFM8886796.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            19184..19525
                     /locus_tag="ACHA5T_001211"
     CDS             19184..19525
                     /locus_tag="ACHA5T_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM8886797.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(19801..21555)
                     /locus_tag="ACHA5T_001212"
     CDS             complement(19801..21555)
                     /locus_tag="ACHA5T_001212"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8886798.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLSFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(21566..22402)
                     /locus_tag="ACHA5T_001213"
     CDS             complement(21566..22402)
                     /locus_tag="ACHA5T_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM8886799.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(22404..23087)
                     /locus_tag="ACHA5T_001214"
     CDS             complement(22404..23087)
                     /locus_tag="ACHA5T_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFM8886800.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
BASE COUNT         7611 a         4143 c         4766 g         6634 t
ORIGIN      
        1 aacgatgtcc ttactacctt attttctatc ttgggtcatt atcagttact ttgtttacgc
       61 ttttttaagt cctgacaaag gaatattcaa tcagtggatc actggacatg gaggagaagc
      121 tgtcaattgg tataatgaac cgaaatattg gccgtttatc ttggtgttca ttggtacatg
      181 gaaagggatt ggttataaca gcattattta ctttgcatct gttatgggaa tcgatccgac
      241 ttattatgaa gctgctatgg ttgatggagc tagcaaatgg cagcagatca aacatgtgac
      301 gataccacaa cttattccac taatgacgat tttaacgatt ttagccgtag gaaacatttt
      361 ccgagctgat tttggcctgt tttataacat tcctaggaat tccggtgcat tatatgatgt
      421 gactcaagtt ttggatacgt atatctacaa tggcctgaca tccacaggag attttggtat
      481 gacggctgct gcaggacttt accaatcagt tgtcggcttt gtcttgttga tgatcacaaa
      541 tacgatcgcc cgacgttttg atagcgattc tgcattattc taaggaggaa aaaaatgaaa
      601 aaaaagaaag tggagagagt ccaagtccgc tctttcaata agacggctaa ttttttctct
      661 aatataatga ttgcactctt tgctatttcc tgtattttgc catttatatt tgttatcgcc
      721 atctctttta ccgatgagtc catattgtcg actcacgggt acagtttttg gccagaaaag
      781 ttcagtatat ttggttatac gtacttgttc gaccagatgc aggataaaat tttccaagcg
      841 cttttcgtca ctgtacttgt tactgtttta ggtacactga tcaatagcac ggctacttct
      901 ttatatgctt atgcgatttc acggtcaaac tttccattcc gtcgtttttt taccgttttt
      961 tgtttgatta cgatgttgtt ctcaccaggg atggtggcca attatcttgt tatgacaaat
     1021 cttcttcagt taaaagatac gatttgggca ttgatcttgc caatggctgt cagtcccttc
     1081 aatattattg tgatgcgcac attctttaaa cgttcggtct ctgattcgat tattgaatcg
     1141 gcacggatcg atggagctag cgaactgcga atatttgttc agatcgtttt accgttagct
     1201 gttccaggta ttgcgacaat cagtttattt gccgcattgg gttactggaa tgactggttc
     1261 aatgctttac tctacatcca agatgataag ctagttccac tacagtatct tttgatgaaa
     1321 atccaaagta atattcaata tctgacacaa aattcaggag caggaagcca attgacaggc
     1381 ggattagcct cagttccagg agaatctgcc agaatggcga tcgtagtcat ctcgacgttg
     1441 cctattgcga tcagctatcc atttttccag aagcattttg tcaaaggtct aacaatcggc
     1501 ggagtaaaag aataaagaaa caaaaatata aatcataaaa ttaggaggaa gaaaaatgaa
     1561 gttatggaag aaactagcct ttacaggtgt ttcagcgttg ttgttgggaa cattagcagc
     1621 atgcggaggt tctggttcta aagaccaggc agaagctagc aattcggaca ctttgcagat
     1681 gtatcaaatc ggagataaac cagataattt cgatcaattg atggaagtag caaacaaacg
     1741 aatcgaaaaa gagatcggtg taaaagtcaa tatcaattac attggctggg gcgattacga
     1801 aaagaaaatg aacgtcatca tttcttccgg ggaaaattat gatatcgcct ttgcgaacaa
     1861 ctatgtttcc aatgcccaaa aaggagcctt tgctgatttg actgaattag cgcctaagta
     1921 cgctgaaaaa gcttataatg atttagatga agcatatatt aaaggaaatc tggtcaatgg
     1981 aaaattatat gctttcccag tcaatggaaa tgtctttgct caacaagtac ttacttttaa
     2041 caaagcttta ctagataaat acgatctttc aattgatgga atccaatctt acgctgatgc
     2101 agaaaaagtc ttgaaggaat tccatgaaaa agaaccaaac acggcagctt ttgccattgg
     2161 acaaggcttc aaagtccaag ggaactttga ttatccttta gggaatacgt taccatttgc
     2221 tattgatctg gatggagatg attcgaaaat catcaaccaa tacgataatg aaacgtatat
     2281 cggactgtta cgtacaatgc atagctggta caaacaagga ctcattccaa gtgacgctgc
     2341 aacgagcaat aaggattatc cactagaagg aaatacttgg ctaatgcgtg gagaaacaca
     2401 aggaccgtat gattatggcg atacaatttt gacaaatgca gcaaaacaag aacttgtctc
     2461 aaaagcaatt actgttccac taaaatctac agctcaagca caaatggcaa attttgtcgt
     2521 atcaaatact tcaaaaaata aagaaaaatc agtagaactt ctagggctgc ttaacagtga
     2581 tccagaatta ttgaatgggc ttgtttgggg gatcgaggga gaagcatggg aaaaagtcga
     2641 tggttcagat gataaaatca aattgctaga tggttatcag ccaaatacac atatgtctgc
     2701 ttggaataca ggaaacaaca agattctata tacacaagag tctattacgg atgacatgat
     2761 tgccaaacgt gatcaatcga ttgctgatgc agaaacttca ccgattttag gattcagctt
     2821 caacactgac agtgtcaaaa cagaattaag taatatttct aatgtcatga atcaatattt
     2881 agatggattg aatacaggga ctgttgatcc agatgaaaca ttaccgaaat tgaaagacgc
     2941 tttggacaag gctggctatg ataaggtatt gaaagaaatg caaaaacaat atgatgagtt
     3001 ccgacaagaa taataatgaa taaagacaaa ggggttgtga caaaagacgt gtcacaacct
     3061 cttgttccga ataaacggtg ttcaaaagcc agtttctcgg tattaagcta aatatatgta
     3121 aaaatgtgga aagccatatt cacaaaattt ttcttactac tcgaaactaa gcggctattt
     3181 cacaaactct tggtaaacgg tgttcaatca gctgaccctc ggtattcgct cacaaaacga
     3241 caaaatatga ggaactattt ttccgtttgt ttctctaata atcaggtcaa ggtgctgatt
     3301 gcccaacctt tttttgtata atgagaaagt ataaacttaa tgtttggagg agaagatatg
     3361 ttagggaagg ttttggaaga gttatttatc cggatctggg tcattataaa actaaccttg
     3421 tatttctgga tatatacatt tgccggcggg atcatctttg gattaggtgc ggcatggaaa
     3481 acagtcaatg aactatttta tttgtacgga ttcgagtata aagaaatcac aataaaaaga
     3541 ggctggaata tatataaaag aaatttttta agaggcaatc tgctatttag tttattttta
     3601 tctggtactg ctttattgag ttacaattta tatttatctg ttcaaatcaa agggctcatt
     3661 tttttagcta ttgattttat tttacttttt gccttgctct gcctatttgc tacttatcaa
     3721 tacagtatga ttttagatag tgaatatacg atctctgttc ctaatctgct aaagctaagt
     3781 tttatttcgg ttttttccag cttttcgtct tttctaaaaa taattattgg tagcggtata
     3841 attttaggcg tgacttggca attcaaagga ttgatcctgt ttggtgtcat tggtcttttg
     3901 acggtttgga atggtacgat gacaacacat tggcgtgaag aattagataa acagttggaa
     3961 agttatgaat aagtttcgga ttctattttc aaaaggaacc ttactaaatc gtctgttgcg
     4021 gaaatacgcg gttatcatga tcggtgtaac gatgactgct accgttattt ttagtatcca
     4081 tacttgggaa caaaatcaaa aacaggcaga aaacatgaca agtgatgccg ttcaaagcac
     4141 aagcagaatg ctgaatgata aaacaacctt gagtaggatc atcaaaaacc agcttgtagg
     4201 gaattccgaa aagatcgaaa atgtgacgac ctacttaaca aaacccatag atcagtatct
     4261 gatgtatgta tacgaacagc aaaacagtac cgatgaatta gtttctttcc caaatcaaat
     4321 caaagatctc tacgcaaatt atgaagaact aagtgcgata tatatcgtgt tgaatcaact
     4381 accggagtat tatgaatctt ctagagaaaa caaaggtggg gaaataaaaa aggggacacc
     4441 aaaattacta gacgcctttt atatcaagtt gcctatcaca caaggaggag ctgagtcagg
     4501 aagtattttt attggttttc aaaagaaaga actagatatg attttagaaa atctaacgtc
     4561 attcaatgga ctctctttat acatgatttc tggaacgaca aatcgactat acacattcca
     4621 tgataataaa atagctaaag aaacgtttga aagacaagaa atgatcatca cagagagtct
     4681 gaagaaaaat tcacaattgc cgattgacga tctggaaaaa aacaattttg ttcaacacca
     4741 agaactgtct gatgattatc gtatattagc tgtactagat aaaaaaagtg taaaaatcga
     4801 aacgggaaaa aatcttgccc cgctgatcat tggtatcatt attttagaca atatactgct
     4861 tatctttctt tataaaacat tcaaacgata ttcacagcaa gtatctatcg tcatgcaggc
     4921 catggatcaa acagcagcag ggaatttaga gacgcggatc gacataacaa acacggaata
     4981 tgaactgaag gaactttcaa tcggtatcaa cgaaatgttg gatagcatca atcagtttgt
     5041 agaagacatc tacaaattag aaatcaaaca gcaagacgca catatgcggg cgcttcaagc
     5101 acagatcaat ccacactttt tatataatac attagaatat atccgaatgt atgcaatcag
     5161 cgaaggaagt gaagaattag cagatgttgt gtatgcgttt tccgctttgc ttcgaaacaa
     5221 tacgaatcaa gagaaaacga tcactttaaa agaagaactg gacttttgcg aaaaatatgt
     5281 ctatttgtat caaatgcgtt atcctaatcg agtagcctat cattttatga tcgatcctga
     5341 tttggaaaag atcgaagtac ctaaatttgt gatccagcca ttagttgaaa attattttaa
     5401 acacggtatt gattttactc gattcgataa tgcgctaagt gtcaaagtct tgcaggaagg
     5461 caaacgggta aggatcatca tcaaagataa tggaaaaggg atgactgaaa aacggctaaa
     5521 acagattgaa gaaaaactgt ctcatcctaa agtggaactt cacggttcta tcggtctgca
     5581 aaacgtgaat gaacgtttac gtgccagctt cggcagcagt tattatatgt cgcttgagaa
     5641 taatgagaca ggtggactga ccgtttctat tacattcaag gagggatgag cgtgtatcaa
     5701 gtgttattgg tagatgatga atacatgatt ttgaatgggt taaaaaagat aattgattgg
     5761 caaagtttag gctttcaaat agtagcaaca gcagagaatg ccaaagaagg actagctgta
     5821 cttgaacagc gacagattga tttagtagta acggatgtga caatgccaga aataaatgga
     5881 ctggaattta ttgaagcggc tcaaaaagaa cgtcataatt ttgaatttat gattttatca
     5941 ggataccaag aattcgatta tttgaaagga ggtatgcaat taggcgccgt gaattatttg
     6001 atgaaacccg tgaataagtt cgaattggtt gaaagcctca agaaaatcaa aacacggtta
     6061 gatcagcaaa atgaacaaaa aaatcaacaa gaaatctatc aagaactgtt attcagtcaa
     6121 tggttgaatg aagaattgga tgaaataagt gaagaagaaa taataggaca aataagtgag
     6181 aagcagcata ggatagtatt gattcaactg gcaagaactc atgatactct tatcaataga
     6241 tggttgcaag aacggcaaca aatcttttac tatcaacgaa attacggaga ccgtgtattg
     6301 atcacgttgt tgttggaggc agatgaagtt gatggcttct gttgttttgt tcaagaaaac
     6361 tttcctgatc aggaatggct gatcagtatc ggggaagaga cagattcttt ggacaaaatc
     6421 cctgaaacat tccaacaagc gaaagacaat ctacagttgc atcaatttta tggagataaa
     6481 gacgagcatg ttttttacgc agtacaagca gaaacaaaag atcaaaccat cgatttttca
     6541 agtttcagac gcgtgctgca aaataaacaa ttggaagtcg ctcaaaaaat gatcactgat
     6601 ttttttgaac agtttcagtt agcagccatg atgccggaag atatacgata ttccagtttt
     6661 ttactgttta tggagattca acgggaattg attgatctgg aagatgagga atatttacaa
     6721 ggaatcgaaa aaatccatca ggcaaaaacg gtgcaagaac ttcagcagct acttctgtca
     6781 ttcatcaaaa agcatcagag acagaaaaag tacagtgaca atgtggaaaa agtaatcgta
     6841 atcctgcacc agcattatca agaaccactt acactcaaag aagtgtcaga aaacctgcat
     6901 ttaaatgtca tgtatctagg acagctgttc aaaaaagaaa cgaaaaaaag tttttcagct
     6961 tatttgaacc atttacgtat ggaaaaagcg aaacaactgc ttttgcacag taatcaaaac
     7021 atcaatgaga tagcaagtga gattggctac aacaacacca cgtatttctc taaattattc
     7081 aagaaaatcg tcggcagatc ccctaaagag taccgagaaa acagtggaga atcataaaaa
     7141 aaaagccaat gtaatgaaat aacttataaa aaattgtcta gaaatctaat tttgggctgt
     7201 tcttattact tgaggcaagg cagctttttc atgacctctc gataaacgga gttcaaaagc
     7261 cagtttctcg gtattaagcc aaatttatgc aaaaatgtgg aaagccattt tcacaaaatt
     7321 tttcttaata ctcgaaacta aacagctttt tcacgacctc tccactaaat ggtgttcaat
     7381 cagctgaccc tcggtattag ctcaaaaaat ggcaaaatat gaggaactat tttgccattt
     7441 gtctctctaa tactcaggtc agaacgctga tttcacaacc tctcaataaa cggtgttcaa
     7501 aagcttgcat ctgcggtaat aagaccaacc aaacgaaaaa tatgaggatc tatttttgct
     7561 tggctgttct tattacttga tgcaggacag ctttttcaca acctcttgtt aattatagtt
     7621 tgataaggaa aaaggcaaat cttttgcttg atatttctta gggtgaggga tgatccctaa
     7681 ttggtaagag atcgtgccgc ctttcagcaa atcttgatgg gtaaaatata atttcgtata
     7741 ttctctgtcc atccgtttga ttttatggat aaattgttgc tgcggccgat taggcgttgc
     7801 ttcaatcgtc aattcctttc catttgacaa aagcaaaaca gccttgtcaa ttaatggcat
     7861 accgataaca tattcaccgg ttccaggcgt gacaggataa aatcccagac tattgaagat
     7921 ataccaagcg gccatacttc cattgtcctc atctccaggg taacccattg gagaatcgtc
     7981 gaaggtctga gtcatcaact gtttaagcag tggctgtgcc atttcaggtt gtcctatata
     8041 actgaacaag taaggatagt gaaaacttgg ctggtttgaa atagctaatt gtccaaattc
     8101 tatcgcggcc atctcactca tttcgtggat ctcaaaacca tatcctcctg ggtcaaacgt
     8161 tgggggctga ttgcacagct caatgagttt ttctttatat ctctcactgc caccatattt
     8221 tccgatcagt cctgaaaaat cgtgataaac agcaaagctg ctttgccaag cacttccttc
     8281 tgcataatct tgtccccaac gattagctga aaatggttct ctgaacttgc catctttatc
     8341 ttttgaacgc ataaatcctg actcatagtc aaagatattt tgatagttca atgattgctg
     8401 ctgatatttc ttcgtcattt cctcttcttc tagtaccttg gcaacttggc taatacaaaa
     8461 atcgctataa gcatagtcta atgtgtgatt gactgattcg tggtagtgat taggaacata
     8521 gccatatgtc aagtaatctt ctgttccttg gcgtccataa tttggatgtt tgctttgaac
     8581 cgtagcacct ttttccattg ccgttagaaa ttcaggcatc aagtctcgtc ggatatcttt
     8641 tacagcagca tctgcaatga ccgcatcgat caatgtccca ggcataagtc cacgttcatc
     8701 aggagacagc cattttggca aatagccact ttctctatag ctgttcaaaa aaccttccag
     8761 catttcttcg tatttttcta cagcaatcaa agaataaaga ggataaaccg ttttatacgt
     8821 atcccaaaac ccattatttg tatagagaac accaggtttt atagatcgac tagtcgtatc
     8881 atagtggatt ttttgcccac taggatcaat ttcataaaat gtttgtggaa ataaaaacac
     8941 ccgatataaa ttatgataaa acgtccgcaa ttgttgttga tcatggtgtg tgagctgtat
     9001 acgatcaaaa taggactgcc attgttcttg actgtgagca aggtaagtct ctttgctttg
     9061 gttttgttct agttccaggt tataagctgc ttgttccgag ctgataaaag atgtcccaaa
     9121 tcggatagtt tgtttttttg tctgttcaaa atacaacggg atcgagtcgc tatttttgtt
     9181 ggaaacagct aactcatcaa ttgttaaagg atattcaaat ctcaatataa aataaaaaga
     9241 aaaatcagga tcttctgaac cagcaaaatg tgtgattttt ccttccactg tgtaaggatc
     9301 tataacaaag agctgatggc gtcccggaaa ggataaaagt aaaccgctgt catttttgct
     9361 gtaatcaata gataagattc cgccatacat gctaggtatc aatgtagaac agatgccata
     9421 acgtaattga cagatcgaca gatgagtcgg gcaaaatata ctttcttctg ggcggtaaga
     9481 actttgagca tggaaaaggg tgttctcctg tagttttcca ttgatcggcg taaacagcat
     9541 atgactgaaa tcacccatcc aaggacttgg ttgatgggta agacggtagc cttgaaaaac
     9601 gcggtcatca ggatgaaacc accagctgcc gttttgatcc gtagtttgcg gtgcaaaata
     9661 attcatccca aaaggaacgc cagtataagg ctggcagttc ccattagaga aatttggctg
     9721 attcgctgtt gcatgtctag tatctatttg accgatattc attttatttc tcctttgttg
     9781 attgtctttt gatgaatgaa actggaaatt tactatcgga tggtcgatct tttgtctcta
     9841 accaagtgac caaagcatct cccgcaagct tacctaactg ttcaaaatct tgtgcgatgg
     9901 ttgtcagtgg cgggtcaaca agagctgctg cttggatatc atcaaatccg ataattgaaa
     9961 aatcttcagg gatatgataa ccatgctgtc tcaagagatt catggtctgg atagcgacaa
    10021 catcgttttc acaaacaaaa gcagttactt catacttagt gatataagaa agcaattcat
    10081 ctaatgtcgc gcttttgtca tccagtgaag tgtggaaagt caaaccagct ttgtccaatg
    10141 cttcaaggta accaaggtaa cgctgtcgaa cggattgtgg atgagtctgc gtaccaaaca
    10201 aataagcgat ttttgaatgt ccctgttcga tcaaataaga ggcagcaagc gcaccgcctt
    10261 ggaaattatc ggaaagaacg gtaggaaaag gtaactcata gattttttta tctaatatga
    10321 cgacagggaa ttctttcaat gaaagctcaa aaagtaagtc aagatgttgt tctgtatcaa
    10381 gagcataata aatcaatccg tcaaattcat tgattagctc ttttgctgtt ttattttgta
    10441 gaaagtcaag cgtcgtcatc atgacatcaa tctgtttatc ttgcatcaca gggttcaatc
    10501 ccttgctaaa atcaccaaca gacaaatcag cgacatatgg aagaagaaac agaatccgtc
    10561 gtgtttttgt aggagaagca agaatctgtt ccggagaagc ggatttaacg aaactgcctt
    10621 tcccgcgaac gcgataaata agtcctaatt gctctaattc agtcaatgca cgtttggaag
    10681 tgattctgct tactttatag gtacgtgaaa gttctttttc agttggtagt tggctgtcta
    10741 caggtaattc accagttaaa atagccgttt gcaaatcatt aaatattgtc tggtacaatg
    10801 gttgagtcat ataaacacct cttaatgata tatcatattc aaataatacc ataatggatg
    10861 aaaaagtaaa taaaaagata ttttttgaaa gcgtagacat aattggctgt atgaagtata
    10921 atctttaaag atatatcaaa tagaaagaag gttttattag tggtttataa agatatacca
    10981 aaatcggtgc agaaatttat ggaagatatc acacaaaaat gtggggatat ccatgcggac
    11041 tgggcaaaga attttacagc agcatttgcc aatactctgt tgacaacagt caaaaagcaa
    11101 aatgacggaa ccaccttttt attgacggga gacattccag cgatgtggtt aagagactct
    11161 actgcacaag ttcgtcctta tttagtgatt gcaaaagaag atgaggaatt agctgacatg
    11221 atcagcgggt tagtcaaaaa gcaatttttt tatatcaata tcgatccata tgcgaatgcc
    11281 ttcaacgaag aagctaacgg tgctggccac caaacagatc atacaaaaat gaacgattgg
    11341 atctgggaaa gaaaatatga aattgattct ttatgctacc caatacagtt ggcctatctt
    11401 ctttacaaaa atacaggaaa aactgatcaa ttcaatcaat cttttattga aggtatcaaa
    11461 aaaatcttgg acgtgttcaa aacagagcaa gatcatgaac actctcctta tacatttgta
    11521 cgagacacga caagaaaaga agatacgctg atcaatgacg ggcgaggtgc aaaaacagct
    11581 tttacaggaa tgacatggtc tggtttccgt cctagcgatg atgcttgccg ttatggatat
    11641 ctggtaccat ccaatatgtt tgcggtggtc gtgttaggtt atgtggaaga aatttttcaa
    11701 acgatactcg atgataaaga aattgtacag caaacacaga aattgaagaa agagatccaa
    11761 gaggggattc agaatcacgg acgaacggtt aatcaaaaag ggcaaacgat ttatgcatat
    11821 gaagtagatg gaatgggaaa cgccacagtc atggatgaca gtaatgtgcc caatctgatc
    11881 gccgcacctt acttgggata ttgttctgaa caagaggaag attatctgca aacaagaaga
    11941 acattgttga gtaaagaaaa tccatatttc tatgaaggaa agtacgcaaa aggaattgga
    12001 tcctcccata cgccagaaaa atacgtttgg ccgattgcat tagctatgga aggaatgaca
    12061 acgtcagaca aatctgaaaa agagcggata ttgaatttac ttgtagcaac tgacgcagga
    12121 acacacctta tgcacgaagg attcgatgtg aacaatcctg aaaattatac gagagaatgg
    12181 ttttcttggg caaatatgat gttttgcgaa ttagtcatgg attactttga tattagagta
    12241 gaaaagtagg aggtatcgta aatgaaaaag aaagtatata tcatttctca tagccactgg
    12301 gatcgtgaat ggtacatggc ttacgaacag catcacatgc gactgattga attgatagac
    12361 gatctactag agctatttga agttgatccg tccttcaaca gttttcattt ggatgggcaa
    12421 acgattattt tagatgacta tcttcaagta aggccagaaa aaagacaggc aatccaacaa
    12481 gcaatcaatg aaggaaaatt gagaattgga ccattttata tcttgcaaga tgattttctt
    12541 atcagctcgg aatcgaacgt gagaaatatg ctgattggca tggaagaaag ccgtaaatgg
    12601 ggaacccctg tcatgttagg ctattttccc gatacatttg gaaatatggg acagacgcct
    12661 caattaatga aacaagctgg tatttcagca gcagcgtttg gccgtggtgt aaaaccaatt
    12721 ggctttgata atcaagtttt ggaagcagaa aactattctt cccaatactc agagatgtgg
    12781 tggaaaggcc ctgatcaaac agcaatcttt ggtctgctat ttgccaattg gtatagcaac
    12841 gggaatgaga ttccagtaga aaaagaagca gctcttgcct tttggaagca aaaattagcg
    12901 gatgcagaac aatatgcctc tacgaaccac ttattgatga tgaatggtgt tgatcatcaa
    12961 ccggtacaaa aagatatatc aaaagcaatc catctcgcaa atgaactgtt tccagattac
    13021 gagtttattc attctaattt tactgactat ctggaagcag ttcaaaaaga tgtgccagaa
    13081 gacttaggct cagtagaagg agaattgaca agtcaagaaa cggatggctg gtatacttta
    13141 gccaatacag catctgctcg ggtttactta aaacaatgga ataccaaggt ccagcgacag
    13201 ctagaaaata ttacagaacc acttgcgacg atggcttatg aagttagtgg aaactatccc
    13261 catgatcaat tagattatgc ttggaagaca ttaatgcaaa atcatcctca cgacagtatt
    13321 tgcggttgtt cagtcgattc tgtccatcgg gaaatgatac ctcgttttga aaaagcagat
    13381 gaagttggaa agtatcttgc acaagattcc ttagaacagc tgaccgcagc aatcgatacg
    13441 accggattcc caaaagacag ctttccattt gtcattgtaa atactgcagg aatggataaa
    13501 actggtgaag cagaaataac catcgaatta gaaagaaaaa gattcgctga aggaatacct
    13561 gaacaactat atcaagagtt agaaaatttg cctaaacgta agtatcatgt agaaacaaaa
    13621 agtggggcca ctataccagc tattttatct gaagaaactg tccaatttgg ttatgatttg
    13681 ccgaaagacc gtttccgtgt gccatatatg gcaagaatga tcaaagtgac tttaccatta
    13741 gaaaacatgc cagcattttc ttgggaaaca ttcgcgcttg tagaagggga agcgaaagca
    13801 gaagaaaagg aaacaatgat tcatcagagt ggacgaatca tcgaaaacgg ccctctacat
    13861 ttgacgattg aaaaaaatgg taccattaca atggaagatc gcaaaaacaa acgaaagttg
    13921 aataatctgc atatttttga agatatcggc gatataggaa atgagtatat ctttaaacaa
    13981 cctttctgtg atcagccaat cctatccagt aacaaagaaa actctgaagt aaaagtatta
    14041 gtagatacac cggaaatagc aaaaataagt attctacagg aaatggaaat ccctgtttct
    14101 gcagacgagc gtttagaaaa agaacagcag atggttgtgg aatttcgtta tcgaaaagca
    14161 gaacgatcca aagaaaaacg aatccttcaa ataaaaacaa tcatgacaat cagaaaagat
    14221 agtaaaaaaa tcgactttga aacgactctc gacaatcaga tgaaagatca tcgcttacgt
    14281 gtcttgtttc ctacaaagtt acatgtggaa catcacgaag cagatagtat ttttgaagtg
    14341 gtgaagcgcc caaaccatgt gtctaaaagt tgggaaaacc ccacgaatcc gcaacatcag
    14401 caagcatttg tcaatattca tgatgaagag tacggtgtaa cagtcggaaa ctttggtttg
    14461 aatgaatatg aagtaacaga agacggacaa atcgcagtga cgctacttcg cagtgtggga
    14521 gaactaggcg attggggata ttttccaaca ccagaagcac agtgtctagg tgagcatcgg
    14581 ttcaattaca gcattgaact acatggacca gaagaaaaat tctctactta tttgcatgca
    14641 tacgcagcac agattccttt ttccactcaa caaatcaaac atcatgaagg aacattgatt
    14701 tcaaaacaac aatatttaac gataaaaagt gaaacgtttg caattacagc tttgaaacgc
    14761 agcaagttta gtgataaagt agtagtaaga ggtttcaata tgtctagtca tttagaaaaa
    14821 ttggaaataa ctaaagacaa tggaaaaact gtcatattga atctgcttga agaaccaacg
    14881 aagcaagcgg ttgtcccaat aatccaacct tatgagattc ggacaatcgg attcgaggag
    14941 gaaaacgaat gtatggcgga atagaagcag gcggaacaaa atttgtgtgt gcggtttcag
    15001 atggtggaga aattttagaa aaattaagta tccctaccac aacaccagaa gaaacattgg
    15061 atcaggtatt tgaatttttt gatcgttatg aattgaaggg aatcgggatt ggctcattcg
    15121 gcccgattgg tatcgacaaa acaaaagata attacggata cgttttggca actcccaaaa
    15181 aagggtgggc acaattcgat tttcttggca caatcaagga acgctacaat attcctgctg
    15241 cttggacaac ggatgtcaat gctgctgcat atggggaatt gaagcaagga gcagcacaag
    15301 gaaaaaatag ttgtatttac cttaccgtgg gaacaggaat cggtgcaggt gtcgtgatca
    15361 atgaagaaat tttttcaggg atcgcacatc ctgaaatggg acatatttgg gtgaaacgcc
    15421 atccagatga ccattatgaa ggtacttgtc cttatcataa agattgttta gaaggattag
    15481 cagcaggacc gtctattgag gcacgcacag ggatcaaagg gcaagatctg ccgcaagatc
    15541 atcctgtttg ggctattcag gcttattata ttgctcaagc actgatcaac tatacgttga
    15601 cgttagcacc tgaaaagatt attttaggcg gtggggtaat gaatcaggat catctccttc
    15661 aaaaaatccg tcagcaattt gttgaattga tgggtggtta tatggaaaca cctcaagtaa
    15721 gtgaatatat cgttcgttgg ggactgccga atgaaagtgg gattatcggt agtttgctgt
    15781 tagcagaaca cgaacatcaa aagtaagcag acaaattaca caaaaagaac agagtcttat
    15841 cattatctgc tgataagact ctgttctttt ttatttctat tcccataatc caggtaaagc
    15901 tttaccgact attttttctt gagtaaatgg atggatcatg tgcagttcta atgcatggag
    15961 catcagtcgt tcagctgttc tattttggta gagagggtct ccaacgatcg gatgtccctt
    16021 atgagctaaa tgaactctga tctggtgtgt acgtccggtt tccaaaacac aatagacttg
    16081 tgtttgttca gaaagtactt tgttgacagt gacatgtgtc aaggcgctct gaccattttt
    16141 atcatctacg accctttttc tccgatcatg gcgatctcgt ccaattttat tggtgatggt
    16201 catatctttg gcaatttctc cccatacgat ggcttgatat ctccgataga tcattttttg
    16261 ttccaacatt cttccaagta tcggcaagac aaatggattt tttgcaaata aaacggcacc
    16321 gcttgtttct ttgtccaagc ggtggacaac gtaaggtcgt tgctgtttag tgtcgagata
    16381 agcagctaaa tgattgagta aggtactgtt ttctgtcggt tcatttggat gagtcttcat
    16441 tcctgcaggc ttatccacta taatcaaatg ctcatcttca tataaaacac ggatgtgctt
    16501 cgcatctcct aattgaactt cttgataatt ataatctgtt tcttcgaata acaatgtgat
    16561 ctggtcattg ccctttactt catgatggaa caaagcaggt tgttgattga tccaaacgtt
    16621 tttacgtata cgcaaaaagt gtcgaacctt tctaggaact aaccattcct tttcaagcaa
    16681 gtcgcgaatt gttgtttcag gatggttcat aggtaaagtg atcgtgattt tcacaagtgt
    16741 actcctttca gtgcaattgc agactaattg taccattttt aaaacatatt taagaaaaaa
    16801 cttacatttt tctgttcatt cttatgttac tctacttgca ctaacgtaaa aaaacatatt
    16861 gaactacaac ttaagcagga agaaaacact ctttcactat ggtaaaataa gacgattgaa
    16921 tcaaatagac aggagaagaa gcaaatggat tttcaaagca ttttagaaaa gataaaagct
    16981 gctttgatca gtttttggcg ttggatcaga ccatatctcg gacaattcca tcgatggcga
    17041 aagcgcatct ggaaaaaata tcatgtgaac aaattgattt tgctgattgg tttgatctgt
    17101 gtcttagtga caagtatcta cctctttatt ttagcaaaac aagcaaatgt cgaaacgtta
    17161 aaatcaggat taagccaatc gacagtcatt tatgataaga aaaacgaaga agctggcaca
    17221 ctgtacgccc aaaaaggaac atatgtagaa ttaaacgcga tttctcccta tatccaagat
    17281 gcagtaatct cgacagaaga taggaatttt tacgagcatc acgggtttga tatcaaaggg
    17341 attgcccgtg cagcagtccg aatggtcttg tctggcggaa catcaggggg cggaggaagt
    17401 acgatcactc agcagcttgc caaaaatgcg tatctgacat tagaccaaac gtttgatcga
    17461 aaagcgaagg aattattttt ggcaatcgag atcgaaaaga agtacagcaa agaagaaatc
    17521 ttgaccatgt atcttaacaa tgcctatttt ggcaacgggg tatggggtgt ccaagacgct
    17581 tccagaaagt attttggagt agatgcaacg aatgttacgc tgtctgaggc tgctactttg
    17641 gcaggaatgc tgaaaggtcc tggtatttat aatccgatcg atcacccaaa aaatgcgaat
    17701 gatcggaaaa acacagtact aagtgtaatg gaagaaaacg gaaaaatcac aaaagaacaa
    17761 gaacaagcag aaagccagac agatatcagc gcgtatctta atgacacata cgataattca
    17821 gattctggtt atcgttatcc ttattatttt gatgcagtga tcgatgaagc agttaacgag
    17881 tatggtcttg atgaagaaga tgtcatgaat aaaggctata aaatctatac agctttagat
    17941 caggattatc aaaagcaatt agaagctact tatcaaaaca gtgcattatt cccagcaaat
    18001 gcttcagatg gtgccatggt ccaatctgct tcagtagctt tagatccgaa tacaggtggt
    18061 gtacaagcac tagtaggacg aagaggagat catgtcttta gaggattcag ctttgctaca
    18121 cagatgcgtc gttcaccagg atcaacgatc aaaccattaa gcgtatatac accagcactg
    18181 gaagctggat acaagccttc cagtatcttg aaagacgaac cgcaaagtta ttacgatgcg
    18241 cataattttg acgggacata ccaaggagaa gtaccgatgt atcaagctgt tgcacaaagt
    18301 ttgaatttgc ctgctgtatg gcttttgcat gagatcggtt tacaaaaagg atatgataaa
    18361 gcagaagaat tcggcctgcc tttagcaaaa tctgataagt attatggctt agcccttggt
    18421 ggactggaaa aaggagtatc tccattgatc atggcgggtg cttacagtgc atttgccaat
    18481 gatggtcaga tgtatacgcc gcatcttatc acgaaaatcg ttgattcgac tggagcggtg
    18541 atcgtcgata acacaaacaa aaaaccaaaa caggtcatat caaaggaaac agccgatgaa
    18601 atgaccagca tgcttcttgg tacattttca aatggaacag cggctgctgc gaatcctgca
    18661 ggttatacga tagcagggaa aacaggaacc acagaaacta atttcgatgc cacaaaagca
    18721 aatgaccaat ggatgatcgg ttacacgcca gacgtagtga tttcgacatg gatgggttac
    18781 gaagaatcca gcaagctgca ctatttggaa gggacaagtg gaaatgttgt cgggaaagtc
    18841 ttcaaatcag cggcagaagg catccttccg tatacttcaa aaacaggatt tagtgtagca
    18901 gatgcctatg ccacaggagg acaagtggta gctgcagatc aagttggaaa cacaggaaat
    18961 tctaatggag agtctggaaa ttggcaagat aacttgaatc aatatggtga acaagcaaaa
    19021 gaagggctga aaaacttcgg cgatatggtg aaagaaggcg ttcagggaat tggtgaagcg
    19081 gctaaagacc tctggcggaa atatcaagga gagtagcccc tttgaaaacg aataacccca
    19141 tgttacaata gagagtaact actaaaagaa aagagggcat aacatgtcaa acatctacga
    19201 tagcgcgaat caaatcgaac gtgagatccg tgaattacca gaattcaaag cgttgaaaga
    19261 agcgtacgag aaagtacaag caaacgaaga agcacacaaa ctgttcaaag acttccaagc
    19321 gatgcaaatc gaattacaag aaaaacaaat gagtggacaa gagttcagcg acgaagatgc
    19381 aatgaaagca caagaaatgg ctgtgaaaat ccaatcagaa gaagtaatca atgacttgat
    19441 gcaaaaagag caaggcttca gtacaatcat caatgacttg aaccgtatca tcatgacacc
    19501 agttcgcgac ctttacagcg aataaaacta ttttatgaaa caacagaggg tgtgaaagag
    19561 acgttcggcc ttgagcgatg acaaaattgc ttcttattgc cgataggctg tctcttgaac
    19621 accatttacc aagaggtcgt gagcctaccg tttaactctg agttacaagg aacaatttca
    19681 aaaaacagct tctcatgttt gatgactatt gtgacttgtg accgaggagt tggcaggaga
    19741 acaccgttta ttcagaaata ggagctacga caaaagtctt gtcatagctc ctattttttg
    19801 tcattcattt ttcaagcggt agaaatagcc tttttgagct aacagttttt cgtaagtacc
    19861 tatttcaacg atttttcctt catcgatcac aacgatttta tctgcctctt tgatcgttga
    19921 aagccgatga gccacaataa aagtagtggg tgtttctgtc aggtaattca gggcttgctg
    19981 gatttttttc tctgactggt tatctaaagc tgaggttgct tcatctagca aaatgatttt
    20041 tggctgacgt atcaacgctc gtgcaatgga gatacattgc ttttggccac cagacagctt
    20101 gttgccatgt tcgcctacta aggtatccag accatcagga agctggtcaa tcagtgaaga
    20161 aagttgcgca gcttcgatga cttcatctaa acgttctttt gatacgtttt tcaagccata
    20221 agtgatgttc tctttgatcg aagcggagaa caacaaagtg gtttgaggaa caacggctaa
    20281 ataatttcgc acacttcgca tatccagttc atccaaaggc tgatcatcta gaaaaatttt
    20341 tcctgaagtc ggctggataa atccaatcaa taagttaagg agtgtagatt ttccagagcc
    20401 agatggccca acaaaagcaa ttgtttctcc agctttcacc tctagagaaa aatgatcgat
    20461 aatcggtttg agtgagtccg gataaagaaa agaaacatcc tcaaaacgaa aatttccatc
    20521 caaatgttgg atttttttct taccgtgata agtttctaca tcgccagcaa tcaacagttc
    20581 gccaatagaa gtgatagatt ccatgccttt tgaaatcgta ggaatcagcg tgatcattcc
    20641 agaaacggaa ttgacgatcg tcgtgaaata cgtctgatac atcacgacat cgccgggttg
    20701 gatgcgccct tgtaaagcta atatcccagt gaaactcaaa caaatcagct gaaatatctg
    20761 aaaactagcc cagctgactg atccgaaaac agattgcagg atatctaagt ggaatccttt
    20821 ttcatagatt tgagtaaaat gcttatttaa gcgagttgtt tcgtgttttt ctaacccatg
    20881 agctcgagca atcggtgcta attcgatcac ttcgatcact tttgcagaag tctcttccat
    20941 ttcttgcctg aagtctgagt tggactgtct gatcctttta cggaaaaaga gagtaacgaa
    21001 tgcggcgaga ggaacagtca ataagaaaaa catgaaaaca acaggacttt tataaagcgt
    21061 gatacctaaa gctacgaata aattgacagc gatatttaat ccgctgacaa atacttgaga
    21121 ggacaacgtt tggatagctt ctacatcacg gatgatcttt gactggattc gtccagattg
    21181 caattctttt tgataaggaa tagatagttc ttggattttc ttgataaggg tcgctcttag
    21241 tccagcttct acttgacgga tcgaagtgct atggtatttt acatgaaaat aattcgtcaa
    21301 tatattttga ccaatcagac aagccatcaa aaaagcgttg aaataaatgg cctgcaactg
    21361 agaagaatct ttcgatgaag ctgcattgat tacattggca atagcgatcg gcattaacca
    21421 agatggaaga tgtttgataa taaaaaagag tcctgacatg gcaaaacgcc aataatttcc
    21481 ctgataaaga tgcagcaatg tctgaaaagc atttgcatga tatttttgat aacttttctg
    21541 tattcgttcc tccataatct tctccttaac aatcttggta aataaactgc aagtgttcta
    21601 ttgcttcagg aacataaatc tcaggagtag cttcaagagt ggcgtacatt agaggacgct
    21661 catatttgat ataatgcagt accgggcgga aatctaaaca gcccttgcca acaggtacca
    21721 tgcggatctt tccattttcc atgatatagt ctttgagatg aagcgctgta atataaccat
    21781 gtaaattgtc caaggcacct cggatcactt tttcctgatc agcatgattt tctttatgca
    21841 tcaaattcgc acaatctaaa atgattttta aatttggtga agctacttca tcgattaaac
    21901 gtttagctaa ctgatagtta tagattggat gattgatccc tgcttcaaca gcaacagtta
    21961 cgccaaaatt ttctgcatga gcgactaatt caatgataga atttctggcg atctgataag
    22021 cttcttcagt aaaattttct tttgtgtaac cgacagagac actaccggtt tccgtagcaa
    22081 tcatcttggc ttgataggca gagcacagag caatttgctg tttgaaagtt gccaatgctt
    22141 gtgcacgaac ttcttgattt ttgctggata ggttgatata gcatcctaaa atggaaagtt
    22201 cgatattgtt tttttggaag tattcaccaa aataggcagc actcccaagt gtcatgtcag
    22261 ttaatttaag ttgttcagga aatgcttttt gtggtgcaaa ttggatatgg gacaagtgaa
    22321 gctgtgatac tttttgaacg atttcttcac gatttcgaac atctaaatcg tgggcacgga
    22381 tgcctaaatt taatggaaac atctcaaact cctttttgta aataattagc aagaggcaga
    22441 cgggattctt ttatcaattc aacaattatt cgggcaactt tatcagcgcc ttctggacta
    22501 aagtgggtat tgtcttttag tccttctggg taatttttac aagtatcagg cataagatgc
    22561 aggtagaatt tttttgtctc ttcttttgag aaggtctgga acaattcttg tgatcttgag
    22621 aacacatcta aaagaggaat atggtgtttc tcagcaaatg cacgcatcgc ttttggatag
    22681 tctccaagta tatctggatt caatgttccg ttttctaagt aatctcgtcg cgtcacagat
    22741 gtcagtaaga ccatttggac gttcgctttt tttgcgcatt caaaaaatat ctgcagattt
    22801 tttaaatagc taccgaaggg aagcgtacct ctttcttctt ggattttctg gtcgttatgt
    22861 ccaaattgta caaataagaa atcattcggc tgaaactgtg aagctagacg ggctaacctt
    22921 ccttcctgga taaatgattt ggtgctgcgt ccattctttg cttggttatt gatctggatc
    22981 tcgggtgaaa aataagcaga gattttttct ccccaacctg tctcgggacg cttgttttca
    23041 tctttttctg ccgcagtaga atcaccggca atatggatag taggcatgtt atctcttctt
    23101 tcataaaggt ttataggcaa tgaagcagaa cagcaagaga agaagacgga gcaa
//
LOCUS       DAYYGO010000038        22897 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000038 DAYYGO010000000
VERSION     DAYYGO010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22897
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.038"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(41..3694)
                     /gene="rpoC"
                     /locus_tag="ACHA5T_001215"
     CDS             complement(41..3694)
                     /gene="rpoC"
                     /locus_tag="ACHA5T_001215"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFM8886801.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVKETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(3773..7390)
                     /gene="rpoB"
                     /locus_tag="ACHA5T_001216"
     CDS             complement(3773..7390)
                     /gene="rpoB"
                     /locus_tag="ACHA5T_001216"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFM8886802.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLFALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8118..9086
                     /locus_tag="ACHA5T_001217"
     CDS             8118..9086
                     /locus_tag="ACHA5T_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8886803.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9104..10009
                     /locus_tag="ACHA5T_001218"
     CDS             9104..10009
                     /locus_tag="ACHA5T_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8886804.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGSAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            10006..10812
                     /locus_tag="ACHA5T_001219"
     CDS             10006..10812
                     /locus_tag="ACHA5T_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8886805.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            10969..11937
                     /locus_tag="ACHA5T_001220"
     CDS             10969..11937
                     /locus_tag="ACHA5T_001220"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFM8886806.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMMTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12254..12778)
                     /locus_tag="ACHA5T_001221"
     CDS             complement(12254..12778)
                     /locus_tag="ACHA5T_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFM8886807.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13065..14819
                     /gene="scm"
                     /locus_tag="ACHA5T_001222"
     CDS             13065..14819
                     /gene="scm"
                     /locus_tag="ACHA5T_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFM8886808.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTS
                     ASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSS
                     TTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVI
                     LLVIGLRITFMSYKKSKR"
     gene            complement(14974..15960)
                     /locus_tag="ACHA5T_001223"
     CDS             complement(14974..15960)
                     /locus_tag="ACHA5T_001223"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFM8886809.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(15983..16549)
                     /locus_tag="ACHA5T_001224"
     CDS             complement(15983..16549)
                     /locus_tag="ACHA5T_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFM8886810.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(16563..16979)
                     /locus_tag="ACHA5T_001225"
     CDS             complement(16563..16979)
                     /locus_tag="ACHA5T_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFM8886811.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(17436..19370)
                     /locus_tag="ACHA5T_001226"
     CDS             complement(17436..19370)
                     /locus_tag="ACHA5T_001226"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8886812.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            19672..20607
                     /gene="pplA"
                     /locus_tag="ACHA5T_001227"
     CDS             19672..20607
                     /gene="pplA"
                     /locus_tag="ACHA5T_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFM8886813.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            20697..21800
                     /locus_tag="ACHA5T_001228"
     CDS             20697..21800
                     /locus_tag="ACHA5T_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFM8886814.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            21813..22766
                     /gene="menA"
                     /locus_tag="ACHA5T_001229"
     CDS             21813..22766
                     /gene="menA"
                     /locus_tag="ACHA5T_001229"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFM8886815.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
BASE COUNT         6726 a         4848 c         4471 g         6852 t
ORIGIN      
        1 gttgtgacaa taatttgtca caacctcttt tttaaaatat ctattttgtt tgattgttca
       61 ttgcgtcttc tgcagccatt tgcgcttcaa tatctgaaat actgtagaca ttttcgcttg
      121 ctacaccgac ttcttttggt tccatgtcac gataacgttg cataccagta ccagctggga
      181 tgatcttacc tataataaca ttttctttca aaccaagaag aggatctttc ttaccgcgga
      241 tcgcagcatc cgtcaagaca cgagttgttt cttggaatga tgcagcagat aagaaactat
      301 ttgtttctaa tgatgctttt gtgataccta gtaagacagg tcgagaagtt gcaggaacgc
      361 ctcctgagac tagcgtattg tagttttgat ctttaaagtc tgcgatgtct aataatgtac
      421 ctggtaagat atctgtatca cctggatcca ttacacgaac tttacgcagc atttgacgaa
      481 ccattacttc gatatgcttg tcgccgattt ctaccccttg catacggtaa acacgttgta
      541 cttcacgaag caagtagttt tcaacagata atacatcgcg aacttgcaac aattgttttg
      601 gatcgattga tccttctgtc aatggtgcac cacggtggat catatcgcct tctgctactt
      661 tcatacgtgc tgtataagga acagtatacg tacgtgtatc tgtttttcct ttgattgtta
      721 cttctttggt acgtgttgca ggatcttctg agatatcgat gacatcccct gttacttctg
      781 tgatcactgc ttgccctttc ggattacgtg cttcaaagat ttcttggaca cgaggaagac
      841 cttgagtgat atcgtctccg gcaaccccac ctgtatggaa tgtacgcatc gtcaactgag
      901 taccaggttc accgattgat tgagcggcga tcgtaccaac tgcttctccg acttcaacgt
      961 cagagccagt tgcaaggttg cgtccgtaac aatgtttgca gacaccatgt tttgtattac
     1021 atgtgaagac agaacggatt gtcactgttt cgacacctgc atcaacgatt tgttttgcga
     1081 tgtcttcgga aatcaattgg tttcctgcaa tgatcaattc atttgtttca ggatgacgta
     1141 cttctttacg ggtgtaacgt cccactaaac gatcttctaa tgattcgatg atttcatttc
     1201 cctcgcggat ggcttggatt tcaagacctc ggtcagtacc gcaatcttct tcacggatga
     1261 ttacatcttg ggccacgtca accagacgac gagtcaagta acctgaatcg gcagtcttca
     1321 aggctgtatc ggtcatccct ttacgagccc cgtgagttga gataaacatt tccaagacgg
     1381 atagtccttc acggaagttc gaaatgattg gcaattccat gattcgacca ttaggagcgg
     1441 ccatcaaacc acgcattccg gcaagctgtg taaagttgga aatgttacca cgagctccag
     1501 aatcagacat catgaagatt ggatttttcg cttccaaacc ttccatcagt ttttgttgga
     1561 tgctgtcttt tgcagcattc caaacagcaa tgacacgttc ataacgttcg tcatctgtga
     1621 tcagaccacg acggaattgt tttgtaatag tttcaacttg tttatgggca ttttcaatga
     1681 tttcttgttt ttcattcagt acactgatgt cagcgatccc tactgtgata ccggcatgtg
     1741 tcgaatgttt gtaccctaag tctttcatac ggtcaagcat cttagaagtt tcttttactt
     1801 tgaatcgttt gaagacttct gcgatgatgt ttcctaagtt tttcttcttg aacggtaata
     1861 ctaattcttg ctctttgata tgtgcaggaa tatcagtacc agcttcaaca aagtatttgt
     1921 ccggtgtttg gacggtcaag ttgaagtctg ttggttcatt cagataaggg aattcaggag
     1981 gcatgatctc gttgaagatg atcttaccaa ctgttgtgat catcgtacgt tctttttgcc
     2041 attcagtaaa tggtttttca cctaatgagt tagggttcac accgatacgt gaatgtaaat
     2101 gaacatatcc gttgcgccat gcgataacgg cttcgttttg atctctgaag accattcctt
     2161 cgccttcgcg tccttcttct tccattgtca agtagtagtt acctagaacc atatcttgag
     2221 aaggagtaac aactggttta ccatctttag ggttcaagat gttttgagca gctaacatca
     2281 acatacgtgc ttctgcttgt gcttcttcat tcaatggaac gtggacagcc atttggtctc
     2341 catcgaaatc ggcattataa gcttcacata caagtggatg caaacggatt gcgcgtcctt
     2401 gtactaaaac tggttcgaat gcttggatac caagtcggtg aagcgtaggt gcccggttca
     2461 gcaatactgg gtgttccttg attacgtctt ctaaaacatc ccatacttcg tcttcttgac
     2521 gttcgatttt acgtttcgca tttttgatgt ttgaagctag ttcgcgttga acaagctcac
     2581 gcatcacgaa tggtttgaat aattcaatgg ccatttcttt tggaagtcca cattggtaca
     2641 tcttcaagaa aggtccaaca acgataacgg aacgacctga gtagtcgaca cgtttaccta
     2701 gcaagttttg acggaaacga ccttgtttcc ctttcaacat atgagaaaga gatttcaatg
     2761 ggcggttacc tggtccagta actggacgtc cgcgacgacc gttatcgatc aatgcatcta
     2821 ctgcttcttg cagcatacgt ttttcatttt gaacgatgat tccaggtgca ttcaagtcta
     2881 acaaacgttt caaacggtta ttacggttga ttacacgacg gtaaagatca ttcaagtcac
     2941 ttgttgcaaa acgtccacct tctaattgaa ccattgggcg taaatctggt ggaataacag
     3001 ggataacgtc cataaccatc cagcttggtt gatttcctga agcgcggaat gcttctaaaa
     3061 tatccaaacg gcggattgca cgtgtacgtt tttgtccttg tgcagatttc aattcttctt
     3121 tcaattcggc tgcttctgca tctagatcaa cattatcaag taattgttta atcgcttctg
     3181 cacccatagc agcttggaat tcttgtccat attgttcacg tttttcgcga tattcgcgtt
     3241 ctgtcaacaa ttgttttttc tctaaattcg tatttcctgg ttcgatgact acatatgaag
     3301 caaaataaat gatttcttcc aaagcgcgtg ggctcatgtc taatacaaga cccatacgtg
     3361 aaggaatccc tttaaagtac cagatatgtg aaacaggagc tgctaattca atatgtccca
     3421 tgcgttcacg gcgaactttc gaacgagtaa cttccactcc gcatcgatca caaacgattc
     3481 ctttataacg gatacgtttg tattttccgc aggcacattc ccagtctttt gtaggaccaa
     3541 aaatccgctc acagaacaac ccttcgcgtt caggttttaa ggtacgatag ttaatcgtct
     3601 caggtttttt tacttcaccg taagaccagc ttctgatttt ttcgggagaa gccaaaccta
     3661 tttgcatact ttcgaattta tttacatcga tcaaaagggg ttccctccat ttcatttaat
     3721 ggcaaaccgc aagacggctg atcttcgacc gccttgcagc tcatagaccg gattagtcta
     3781 aattatcttc tgcggtttca aagttttgga tgacatcgtc tttttcatct tccacttgtt
     3841 cagcagcttt cttttctaat tctttggcag tttgctgttc ggcaaatttt gtcaaagcat
     3901 caacagtgat caaatcgtcg tcttcatcgt ccatgtcgcg aagttcgatc tctgtttctt
     3961 cgatatccaa gacacgcatg tccagaccta gtgattgcaa ttctttcacc aatacgcgga
     4021 atgattccgg tacacctggt tttggaattg gttcaccctt gacgattgct tcgtatgttt
     4081 tcacacgtcc gactacgtca tctgatttgt atgtcaagat ttcttgtaaa gtataagccg
     4141 caccgtaagc ttccagtgcc catacttcca tttcaccaaa acgttgtcca ccaaattgag
     4201 ctttacctcc caatggttgt tgtgtaacca atgagtaagg tccgattgaa cgtgcatgca
     4261 atttgtcatc gaccatgtga gcaagtttga tcatgtacat cacaccgaca gacacacgtc
     4321 catcaaatgg ttcacctgta cgtccgtcgt acagaacagt tttcgcatcg ctggccatac
     4381 cggcttcacg aactgtttcc catacgtctt catcacttgc accatcaaat actggcgttg
     4441 ctacgtggat acctaactga cgtgcagcca tacccaagtg taattccaat acttgcccga
     4501 tgttcatacg tgaaggcacc cctaaaggat tcagcatgat atcgattggt gttccatcag
     4561 gtaggaatgg catatcttct tccggcataa tacgggatac aaccccttta tttccgtgac
     4621 gaccggccat tttatcccct tcatggattt tacgtttttg aacgatataa acacgaacta
     4681 acatgtttac gcctggtgac aattcatctc cagcttcacg agtaaagatt ttcacatcgt
     4741 gaacgatacc gccgccgcca tgtggtacac gtaatgatgt atcgcggact tcacgagctt
     4801 tttcgccaaa gatcgcatgc aatagacgtt cttctgcaga taattctgtt accccttttg
     4861 gcgtgacttt ccctacaagc aaatcgccat cttggacttc tgcaccaata cggatgatac
     4921 ccatttcgtc taaatctttt aatgcgtctt ccccgacatt agggatttcg cgtgtgattt
     4981 cttcaggtcc taattttgta tcgcgggctt ctgattcata ttcttcaata tggatagaag
     5041 tgtatacgtc atcttttacc aaacgacggc tcatgatgat cgcatcttca tagttgtacc
     5101 cttcccaagt catgaaacca actaaaacgt tttggcctag agccatttcg ccttgttcca
     5161 tagatggtcc gtctgccaga gtatcccctt tttcgacttt ctcacccaaa tgaacgattg
     5221 gacgttggtt gtagcttgtt ccggagtttg aacgacggaa ttttgttaca tcgtatttat
     5281 ctaatgcacc gttgtcgcgg cgtacgcgga tttcagaagc atcaacgtat tcaacgacac
     5341 catcatgttt acataacaaa gcagctcctg agtcatgcgc tgatttgtat tccataccag
     5401 taccaaccca tggtgcttga ggattgatca acggcactgc ttgacgctgc atgttggcac
     5461 ccatcaaggc acggttggag tcatcgtttt ccaagaaagg aatacatgct gtcgcaacgg
     5521 caactacttg tttaggtgat acgtccatgt agtctacttt atcaatagat acttctaagt
     5581 tttcactttg agcacgagcc ataacaacgt cttgagcaaa tgttccatct tcattcaatg
     5641 ggctgtttgc ttgggctacg atgtaatggt cttcgatatc agctgtcaag tagtcgattt
     5701 ggtcagtaac acgacctgtt tcacggtcta cacgacgata aggtgtttcg ataaatccga
     5761 atttatttac tttcgcataa ctggacaaac tattgatcaa cccgatgttt gggccttcag
     5821 gagtttcgat cggacacata cggccatagt gggagtagtg aacgtcacga acttcataac
     5881 cggcgcggtc acgagtcaat ccgccaggtc ctaaggcgaa aagacgacgt ttgtgtgtca
     5941 attcgccaag agggtttgtt tgatccatga actgtgacaa ctgagaagaa ccaaagaatt
     6001 ctttgatact tgccacaact ggacggatat tgatcaattg ttgaggtgtc aatgtttcag
     6061 tatcttggat cgacattctt tcacgaacca cacgttccat acgtgctagc ccgatacgga
     6121 attggttttg taaaagttca ccgactgaac ggatacgacg atttcctaag tggtcgatat
     6181 catcgacatt gccgatacct tccatcaagt tcaagaaata gctcattgaa gcaatgatgt
     6241 cggctggacg aaccgttttt acagctgctt ctggatagcc gtttccgatc acgtttactt
     6301 cacgttcagg gtctcttggt gaaaatactt tgatgacttg gacagtcatt ggatctgtga
     6361 ccacaccgtc ttctgatgga tagtaagtca ctgaatttag gccattatca atgaaaggag
     6421 ctagtgtttc catgacttgg tgagtcagaa ctgttccttt ttcaacgatg atctcgcctg
     6481 tttctggatc aactaatgtt tcagctaatg tcagattcaa tagacgtgtt ttcaaatcta
     6541 attttttgtt tactttgtaa cgaccaacgt ttgctaagtc gtaacgtttt ggatcgaaga
     6601 aacgagcatt cagcaagtta cgtgagctgt ctgctgtttt tggttcgcct ggacgcaaac
     6661 gttcgtaaac gtctttcaaa ccttcttctg ttcttgagtc gcttgcgttt ttatgcaagt
     6721 ctttttcgac tgtattgcgt aatgtttcgc tgtcgccgaa gatttcaaag atcgtatcat
     6781 cggaaccaaa tcctaacgca cgaactaata cggttaaagg aatcttacgt gtacggtcga
     6841 tacgtacata agagatgtct tttgcatcag tttccatttc tagccaagct ccacggttag
     6901 ggataacagt agaaccgaat ccttcttttc cgtttttgtc gacttttcca tggaaataga
     6961 cacctggtga acggactaac tgagaaacga ttactcgctc tgccccgttg atgatgaatg
     7021 ttccttgttc tgtcattaat gggaaatcac cgaagaatac ttcttgtgct ttgatttcgc
     7081 cagtctcgcg gtttgtcaaa cgcaatgtta catgtaatgg tgcagagtag tttgcatcat
     7141 gcgcacgagc ttctgctact gtatactttg gttctttcaa ttcataatct acaaattcca
     7201 aagatagatt gccattgaag tcatcgattg gcaaaatgtc ttcaaacatt tctctaagtc
     7261 cctcatctaa gaaccattgg taagagtctg tttgaatttc gatcaaattt ggtaattcca
     7321 acacttcact gattcttgcg aaacttctac gttcgcgatg ttttccgtat tttactacgt
     7381 gtccagccaa gctcttcacc cctctaaaat gtcattcaaa aagactaccc gatcaattgt
     7441 tacaataata tttcaaatat tatttttagg taacaacaga cattttttgc ggcttttagg
     7501 caaaaaaaaa ccaaaaatag aacattctct tgaaacctta cttcgtgagc ttctactttt
     7561 gtttccttat attaaagccg aaacggacaa tatttcgttt ctgccttttc ttcatcagat
     7621 agtttttgca acttcatata ctacacttat tttctataaa agtcaacact tcctggcgtt
     7681 tcttctgctt tttcgcaaca ttttcttaat gttttcaatt ttatgttttc aattatctga
     7741 caattcttaa ttgacagacc tttttatctc ggttatactt tagaaaattc attttgtgga
     7801 ggaacttcta tgcgaaccaa catcaaccaa atcgcaccaa cacaactgaa taacttttac
     7861 tttagctact ttagataggt ttgtattcat catgatggat acaaacgtta aacgacagtt
     7921 tgtatctatg atggctaaag aactttgtat tgcagcaaaa agccacgtag acaaaatgct
     7981 aagtggctga gcagtgaaaa tacaaagtaa aagtatcaag cattctactt tttcaattta
     8041 ttttccgact tttcaatcca tttggacatt ttgtctgaat ggattttttg tttcccaaaa
     8101 ttaaggagga aaaagaaatg aaaaaaaata taatcatcgg attaggcgct gccctgctac
     8161 tagctttagg aggttgcgca caaaaagaca attccgcaga ggcatcagat ggacaaaaaa
     8221 caattggcgt attgcaactt gtacaacata actcgttaga tgctgcctat aaaggattca
     8281 aagaaggatt agcagaagaa gggtataaag aaggcgataa tctaacaatc gattatcaaa
     8341 atgcccaaaa caatcaagac aacctgaaaa gtatgagtga gaaactcgtc aaagaagatc
     8401 ctgatttgct acttggaatc gccactccag cagcacaatc tttattaaat gaaacaacca
     8461 ctatcccgat gactgtcaca gcggtcacag acttggaaca agcccattta gtcacatcca
     8521 atgaaaaacc tggcggcaat gtgactggca cgacagatat ggtgccaatc gaaaaacaaa
     8581 tcggcttact gttaaatatc gtgccagacg ccaaaacaat cggcatcatg tataacaacg
     8641 gtgaagcaaa ctctaagatc caagctgatt tggcagaaaa agaattgaaa aaagcgggcg
     8701 tcaaagtcaa agtactgaca gcaaattcca caaatgatgt ccaacaagtg acgaaaagtc
     8761 tagctaaaga tgtagatgga atctatattc ctactgataa cacgttcgca agtgcggcaa
     8821 gtgttgtcgg tgaagtcgca aaagaaacaa aaacacctgt cgttgctggc gcagttgatc
     8881 aagtcaaaga cggcggtcta gcaacagtag gaatcgacta cgaaagcttg ggcaaacaaa
     8941 cagggatcat ggcagcaaaa atcttagacg gcaagaatcc aagtgaactt cctgtcgaaa
     9001 cagcgaatga tttggaactg tatgtcaata aagacatggc caaagcatta ggcatcaatc
     9061 cagacagcat caaagaagct gaataaaaca caggaggatt ctcatgacaa atttgctctc
     9121 tcttttctta tcatctgcat cgcaaggggt cttatgggcc ctacttgcga ttggtgtata
     9181 cgtcactttc cgtatcttgg acattgccga cttgactgct gaaggaagct ttcctttagg
     9241 tgcagggatc gcggcagtaa gtattacaaa tggctatcat ccattgacag cttgtcttct
     9301 aggcttttta gggagtgcgg cagcagggct tgtttccgga ctgcttcaca caaaattgaa
     9361 aattcccgcc cttcttgcag ggatcatcac catgactggt ttatattctg tcacatcccg
     9421 tgtgatgggt gcgccgaaca tctctttatt gggtcagcca acgatctttc attgggtcga
     9481 gtcattcggc ataacaaaag acaacgctgt actgatcgtc ggtctgcttt ttgctttgat
     9541 cgttgtatgt ttacttgttt tgttcttacg tacagaaaca ggacttgcta ttcgtgcaac
     9601 tggcgataat ctggcaatga gcgaagcaaa cgggatcaac acagataata tgaagatcat
     9661 cggttatatg atttctaatg gatgcattgc tctttctggc tccttgcttg cgcaaaacaa
     9721 cggctttgct gatttaaatt ctggtattgg aacgatcgta attggacttg cttctattat
     9781 catcgcagaa gttttgttac gtaaccagcc actatttctc cgtctcttga ctgtcgtttt
     9841 aggtgcggtc atttatcgtt ttattttagc gttagtcttc gaactgaatg ttgaaccaag
     9901 tgattcgaaa cttgcttctg ctttcgtgct agtgatctgt ctttctttcc cgcagatcca
     9961 gcaaaaattc catttacctc aaaaaaagaa aggcggtagt gtcaaatgaa tgacgtctta
    10021 acattggaaa atctccatca tacctttgaa aaaggaacga tcaacgaaaa tcatgtcctg
    10081 aaaggcatta atctttcctt agatcccggc gacttcgtga cgatcatcgg tggaaatggg
    10141 gctggtaaat ctactttgct gaacagcatc gccggtacat tttctgtcga tcagggtcaa
    10201 attttattga atggaaaaaa tatcacgaaa aaatctgttg tcgaacgttc gaaatccatc
    10261 agtcgcgtat tccaagatcc taaactaggg acagccgttc gtttaacggt agaagaaaat
    10321 cttgctcttg ctatgaaacg aggaaaaaaa cgcggcttct tccgtggtgt caaaccacaa
    10381 gaccgaacat ttttcaaaga ccagctggtt cggctaaatt tggggttgga aaatcgttta
    10441 tcctcagaaa tcggtttgct gtctggagga caacgtcaag ctattacgtt attgatggcc
    10501 acattacagc gtccagaact gatcttatta gatgaacaca ctgcagcgct tgaccctgct
    10561 acttccaaaa cggtgatgga attgacagaa aagctgatca ctgagcagaa tctcactgct
    10621 tttatggtca ctcatgacat ggaagacgcg atccgctatg ggagtcgact gatcatgctg
    10681 catcaaggaa ggatcgtcgt agatatccag caagaggaaa aacagcagct cacagtgccc
    10741 caattattag cgttgttcaa acaaaacagc ggcacggcat taaaagacga tcaagtactt
    10801 ttatcttcat aatcagaaag aggatgggac aaaaaatgaa cgcccaccct cttctttttt
    10861 ctctttcctt ctttcgtttt tgctttatgt atcaaccgtt gtttcttcga aaactatttt
    10921 gttctctcct tcttcctgat agaatggatg ggaaaggagc ttgatgccat gacaacgaaa
    10981 gacaaggtac ttcaattatt gaaaaagaca ccagattttc tttccggaga aaagttggca
    11041 cagcaactgg aagtttcccg gacaagtatt tggaaagcca tcaaagaatt ggaaaaggca
    11101 ggctatcgtt ttgagcacgg gcctacaggg tatcgttacc tgccctctga cgtactagat
    11161 gcaaaagaaa tccagcaaga gctacggtcg tttattcctg atttgaaagt acaaacgatg
    11221 atgacttctg aatctacgat gaaagatgcc aaactggcag cagctcaagg agattctgtt
    11281 tcgaagttat ttgttgctga tacacaagaa caagctcgtg gacgttttgg acggcctttt
    11341 ttcgctgaac ctggacgagg aatctacatg agtctcttgc tccatccaga caaacggttt
    11401 gatgaattgc ctcaatatac gatcatcgca gccagtgcct gtgtgaaagc cattgaatct
    11461 gtaaccggtc gttctgcttc gatcaaatgg gtcaatgata tctacatgga tggaaaaaaa
    11521 gtctgtggga ttttatctga agcagtcagt gatatggaaa gcggcaggat cagtcatatc
    11581 atcattggga tcgggcttaa tttttctgtt cctcaaaatc attttccagc tgatctccaa
    11641 gaaaaagcca tctctgtctt tcccgacgga caagctagtg tcacaaggaa tcagctaatc
    11701 agcaaaatct ggcaagaatt gttcacttat ctttctcacc cagaaaaagc gttggacttc
    11761 tataaagaaa aatcattcgt attgggaaaa accgtgtcgt tcaatcaaaa aggaacgact
    11821 tatgtcggtc ttgcaaaaga aatcggtgat catggcgaac tgatcgtcca aacaaatgaa
    11881 cagaagatga gtttgtcttc tggcgaaatc agtctcacat ctatccgtac gcaataatta
    11941 tcagatttca caaaaggaca cctgatctca tacagtgtcc ttttttctga attctcttca
    12001 aaagactctc cttattctat cagctgacag tttctctatc ctctagaaaa aggtattcta
    12061 aaaatatcta taaagcatgg tacactttct ttaaatgaat caatgcaaaa aacaagagag
    12121 tcatcagttt acacgatcaa aagagatagc aggccgcttc ctttttcaat cttatagata
    12181 gaccatgggt aacgataaaa acaaaaaaga acttgcgact tattttgccg taagttcttt
    12241 tttagctagt gccttaatct cttaatccca acaaacgtct cgcatagatc acctgaccag
    12301 agtgcatagc agcatcatca ataatagaaa cgagacgcat tcctcgtgtg actggtggtg
    12361 tccagttctc gtcaattact tcatctagct cggaaggttc cagccttctt aagtaagaaa
    12421 tagcaaaatc agcagctcct cgcaagtatc ctagcagatc ttctatttga ttagcttgga
    12481 tctgctttgc ttgttccaga ctatgcttcc aatcttgttc gttatctgct atcgtcatcg
    12541 gaaaccggct ttcccagtct tgactatgcc aaattggaga agttcctgct aaatcagcaa
    12601 tctggaaatc taattctcga gcggtatgcc aagccagcca agtcattgat ttgatctgcg
    12661 gggctctatc ttccactgga aaacgattgg cctcctcaat agagacgcct tcaaacaaac
    12721 gttcaaaccg atcgtatgcc cgatccacac tctcgcatag catatctaaa taatccattt
    12781 attttcctcc tttttattta tcctacttac tttttcctaa ttctaggata cctacttata
    12841 tgttactaca cacccttatt ttttacactt taacagtcac catcatcaat agtttggaga
    12901 ttgaaagaaa aagatagtgt cgaactatca attgaacaag aagcaacaat atgaacgttt
    12961 cattaatatt ctttaaataa acaatgattt ttcaaacgaa cagtttaaga ctaggagaaa
    13021 aagagtataa taggaaaatg tttgaaagga aaggaagggg aaaaatgaaa aaaagggcaa
    13081 gtttactagt cctagttgct ttgttgttca atatcttttc gtatggtgtg gccgtttcag
    13141 cggaaacata cattcctaaa gaccctggga atatttataa tgatctaact ggtaactatg
    13201 gcttgttagg aattgctagt caattccacg tatttgctaa aaataagaca actgtgaatg
    13261 cacatacaga cggaaatata gcaacacatg aacttgatgc aaaaaataat tttggtacag
    13321 agcttcatga aggtttattg aaccaagaaa taaattatat acaaaaaact gattctttcg
    13381 ctaattcttc aggcatacct acaggtagca caagaatgaa taaatttgtc gttggcagta
    13441 acgtggatgt agcacttcaa gatggtcatc ctacagtcaa tggatccagg cttgatcact
    13501 taactccaga tgatttttat aaagatcgtg ctgggcatga atatattgat ttcgagcagg
    13561 aatttgctaa attgaatgta gcagcaaatg atcttgcaac gatcactcca gctaaaacat
    13621 atactgctgc agattttcca gacatgaaca atcggacaat cgatttaact gatttatcag
    13681 ataccgggtt tttattggta aatatcgatg cagaagtgtt aacgatgaat acgccacttc
    13741 aaatcattaa tccaaatgat caagtagttg tatttaatgt catcaacagt tcttctgcat
    13801 tgaacgtaca gtcaccaatc aaatacaatg atcgttcaaa tcatgaaaca gaagattttt
    13861 cggatgcgaa tatctcttgg aattttggaa atgagatgac tgatttgaca atcagtgctc
    13921 cttttcaagg aacaatcctt gcgcctaatg ctactatccg cattactcaa aaccaagacg
    13981 gaacgattat aggaacaaat gtgattttag atgcagctac caatcgttgg gatcctaacg
    14041 aaatatttat cagcgacaat ggtacagata cgacagacac aagcgacagt acagatacat
    14101 ctaccacaag tgatagctca gattcatcaa cttcaagcga cagtacagat actacctcta
    14161 ccacaagtga tagcacggat acatcagctt caagcgacag tacagatact acctctacca
    14221 caagtgatag cacggacaca tcagcttcaa gtgacagtac agatactacc tctaccacaa
    14281 gtgacagcac ggacacgtca gcttcaagcg acagtacaga tactacctct accacaagtg
    14341 atagcacgga cacatcagct tcaagtgaca gtacagatac tacctctacc acaagtgata
    14401 gcacggacac gtcagcttca agtgacagta cagatactac ctctaccaca agtgatagct
    14461 cagattcatc taccactaca agtgatagca cggacacgtc agcttcaagt gacagtacag
    14521 atacatcctc taccacaagt gatagctcag attcatcaac ttcaagcgat agtacagaca
    14581 cgtcgtctac cacaagcgat acacgtcatg caagtgtctc cttctcaaaa acaagttcgg
    14641 ataacaggac gaactttaac ttaactaata aaacaagcaa tgacaatgga aacaaatcaa
    14701 aaagagcact tcctaaaact ggaagtcaat cgaataattg gataacactc gcaggcgtta
    14761 tattacttgt aatcggttta agaatcactt ttatgagtta taagaaatcg aaaaggtaaa
    14821 aaaatattca agataaaaaa gagcaacgtc gctaacggac gttgctcttt atttttgctt
    14881 tttatcataa atacaagaac tacaattcaa tttgtcaaaa aacacgtaga tttccttctg
    14941 agaaatttat ttacgtatcg aacctttccc tattcagtct ttgcgatcca aaatcagttg
    15001 ggtaagggaa aagagattct cttttgttcg aagagaggtg gcaggtaatt tctttatttc
    15061 cttcaaagcc ttttctgtgt aagaggccgc taacttccta gcttcttcca cgcctcctgt
    15121 atgttcaacg attttgcgta cggcgtcgac atcttcttct gtcattgccg ctttcttctc
    15181 taaataaggt agtagctttg gtttgttttt ttgcaaagag tagatcaacg gtaaagaata
    15241 gaccccttga cgcatgtctt ctagtacagg tttgccgatt tcctggctct cttttgtata
    15301 atctaaaata tcatcgataa tctgaaaggc catcccgata gaaagtccga tatctcctgc
    15361 tttttttgcg aagcgctcgc ttgttccact ttcaaaagcg ccaacagaac aacttaattg
    15421 gaacagttca gctgtcttac ctgaaatatt ttccaaatat tcttttacag aaagttcata
    15481 attatagcga tcatccatct gccctaattc gccattcaat actttttcca tactgcgaga
    15541 attcaattgg agacttttca aggaagtgga gtaatcagac aatagtttaa aacagcagac
    15601 aaataaatag tctcctgcat agacagaaac gacattacca aactgactac ggatattagg
    15661 ctgaccacgt cgcagatccg cttcatcaac gatatcatca tggatcagtg tggccgtatg
    15721 gagcatttcg atagaagctg ccaaagcgat ggctttgtct cgatcttgct ctggtccaaa
    15781 ttgtgagaac aatagctgat aggcaggacg caacagcttt ccgccggcat ggatcgtttc
    15841 tttgattgct tgttcgacag gtttgttttt caggcggaca gtttcttcca tccgttttag
    15901 tgtttctgtc aattctttac ttaaagcagg atagttttcc cacattggat gtagcttcat
    15961 aaaaaaactc cttgtgtgac gattattttt tggtaaacat tgggctccat ttttgtttgg
    16021 ttccctgctc ataatctttt tggaatcgtt gaagtgtctg cacatgtcgt aaaagataat
    16081 tcgctgcgat cccgattgca atccctgata aaatccctaa aactgaaagg actggcaagt
    16141 acaacatgac tgtccatgat tgtgcaatga aagaagccat caaaagctgt ccgacattat
    16201 gcatgaaacc gccggccgca ctaattccga tgatactgac acgttttggt cctaattgct
    16261 tgaccaataa cattccaaaa taactaagca aagcaccact catactataa aggaatgtgg
    16321 aaatcgttcc gcctagcaaa gtagtcagga atagtcgtag ccaaagtagt aagaagctgt
    16381 cttgtttttt catggtaaaa atggcaacga tcgtgattag attggctaag cctagttttg
    16441 caccaggcgc aaaagaaaaa ggataaggga tcatattttc caataaacca atgatcactc
    16501 cttgtgcaac aagtagcgaa atataaatca ttttttgtaa tttactcatg gtgtgtaaag
    16561 gtctaataaa tcagactgtc ttcgtcacct ccatctgttg aacggacttc gatcactaat
    16621 ttatgtggca aacagacgat ggtttcgcca tttttcgttg cccagcctcg acgtacgcag
    16681 atctggtcat cgcaatctgc ttcttttatc cgtactcgat cgccatctat ctcgatcaag
    16741 ttataatctc cgtgttcatc tttgtaagta tatgtgttgc tctcttctcc agccatcaaa
    16801 gggaatgttt tgatttctgt gccatccaca cgcagtaccg cttctttttc tgctgttgcg
    16861 gtgttttgct gaagaccaaa aatcatgact ggtaaaaaag aaagaacagt caaaagcaaa
    16921 ataataaaaa catcccaagg tttgagcaga cttttttcag caaattcttt aaggctcatt
    16981 tttgttctcc tttttccaga ttaactcacc caattttagc ataaaaacaa gaggttgtga
    17041 cacctgtgtc ttgctttgag taataagaac gaagaataga aaacagcttt ccatgttttc
    17101 taattctttg ggcttattac cgagaagcag caggtggaac accgtttata acaagaggtt
    17161 gtgacagaag tgggcaactt caagaaataa gaaggaattt acgaaaatta cttttcaagt
    17221 ttttgtgaaa tttcggctta tttttcgaag aagctacttc tggaacaccg tttataacaa
    17281 gaggttgtaa cagaggttgt gacacctgtg tcttgctcat ggaggcttat ctcgggcatc
    17341 ttattccttt aattttacat aaaaaggagg ctgtgacggt ttgtctcagc ccctttctat
    17401 tgaaatcttg cattcaaata acgttcttta tctatttatt tcttgccgta ctgggaactt
    17461 ggtctaccgt accaccaatt acctagtgta cgttgtagcc atggaattac ccagcgatcc
    17521 aacccaatcg cacgtccgga accgttcatc aatgcgaaag caacaaaaat gaaccaaatg
    17581 tttacccagt aaaacatgcc tgataagcag aacgtgatca ccaatacaat cgttgtcgca
    17641 ctgcatagcc atgtgaataa tccagcaatc aatgctaatg ccaaggcaat ttcaaagaat
    17701 accatgaatt tttggaagaa cattgctaca tcattgtttg gcatgatcac ttgcatgatc
    17761 tttgtgaacc agtctggtga cttctcaatc acagccattg gttcttcacc gtatgcataa
    17821 ctcaaaccaa aatgtgcagc ttgagtcgct gcttctccgg cacttgcccc tgctccgctg
    17881 gcagctgtgg ttgcatcacc agcagcagaa gcaccactgg caacatctgt tacattgtat
    17941 tcccatgtcc aagggaacac actgtttcca gtgaaccatg atccttcacc aaaccaagta
    18001 cttggtttga acacttcacc attcccaatg atttttttgg aagcttcaac gaaccaaact
    18061 aaaccataga aaatacgaag tggcacactc cataagacat tgccgtaacg agaagaatgt
    18121 cctcgagtca cgttgcgatc atctttgata tggaaaaact catgcattaa atattggaac
    18181 atataataac cagaacgaat atcaaagaag tatttcaaat tcacgatatg tttcattacg
    18241 atagctaaaa atccgcttaa atggatctta tcaaataggt tagctacccc ccatttggca
    18301 ccaatagaaa ccataaatcc ttgatagttt cctttaaatt tatgcttttc tccgcctttg
    18361 atcgaagcca cgatattcgc tgcggctgtg tgtcctgttt gttcagcagc ttgaacgatc
    18421 tgcggtgtcg gtgttcctgg gaattcttca taaaagacta aatcaccaat gatatagatg
    18481 tttttatctt cgtagccttt tgcctgcata tattcattgg cgatcaaccg ctgtccgcgt
    18541 gctgattcta atccaaagtc agcagcgtca gaagttcctt tgacaccagc tgtccaaatc
    18601 agtgtgtgag tcggaatgtt gcgtccgtct ttcaatttga tatgatcagc agccacttct
    18661 acgattggtg cattcaataa taattccacg ttgtgcttac gcaaatagcg ttcagctttt
    18721 gcagcatcat ttcgatccaa catattcaag atcgtcggca ttgcctctac gaccatcagt
    18781 gtgatttcgt ttggatctaa tttataatct ttggctaggc gatttttcca gtcgatcagt
    18841 tccccaatca tttcaatacc ggtaaatcca gaaccacaga caacgaaagt cagcatggct
    18901 ttacgcagtt catcatctgg ttccaatgca gcttttgcta ctgtcttctc aatatgttcc
    18961 cgaatcctta cagaatcttc aaatgaccaa agggtgaatc cgtgtgcttt gacaccaggt
    19021 gtaccaaagt cattcggctc tccgcccatt ccaaggatca gctgatcaaa aggataggaa
    19081 cctaatttgg tcttgacgat ttttcgttct ttatcgatac cgattacagt gtctgtgaca
    19141 agttgtacat tcttttgacg agcaaacaaa tgctgcagat cgtattggat tgcttctggt
    19201 tctacacgtc cgccagctac ttcatgcaac tcggtcatca tagtatggta cgaatgacga
    19261 tcgatcagag taattgctac atcttctttt ttcagtttct ttgccaagta ttttgtagct
    19321 gaaacaccgg catatcctgc tcctacaacg acaatatttt gctttgccat tgatcatccg
    19381 ctccttatag actatctata taatgaatat atgataattc ttttagtttg atacttatta
    19441 cacaatctcc atatattata tagatgttct gttcttttgt ctaactcctt ttggattttt
    19501 ttcaaatttt aaaataaagc gttttttcac gaaatttgct gaaagaaaac gtttgaaata
    19561 ttacatgggt tatttatcaa aaatagttga cttataggct aaaaagatta gaatgtataa
    19621 tgaatcacaa agaaaaattg tggactagaa atggaatggt ggggtattaa aatgaagaaa
    19681 aaacgttttg taactggatt tgccgtatta gcattttcag ctctagtgtt aggcgcttgc
    19741 ggcgcagatg acaacggaag ctcaaactct tcttctgaat catctacagc acaatcttcg
    19801 actgcaaaaa gcacaacaga atcatctgca aaagttgtag ctggcggaga tttgcaagac
    19861 ggcacatata aattagaaga aaaaaattat tcaaatggtt accgtgcagt ctttgaaatg
    19921 gttgtaaaag acggcaaaat cactgaatca aaatatgata acgtcaatga aaacggcgaa
    19981 tcaaaaacaa aagatgcaga atacaacaaa aacatggaag caaaatctgg cacaaaccca
    20041 gaaaaataca ttcctgagtt gaatgaccaa ttcttaaaag cacaaagcgc aagcggtgtg
    20101 gaagtcgtaa ctggagctac tcactcatct gaaagcttcc aaaactacgc acaacaattg
    20161 atccaagctg ctcaagctgg taacacagat acaatcgaaa tcgacaatgg tgctgatttg
    20221 aaagacggca cttacaagct tgaagaaaag aactactcta acggctaccg tgtgcaattc
    20281 gaaatgacag ttgctggcgg caaagtaact gaatcaaact ttgattatat cgacaaagac
    20341 ggtaaatcaa aacaagatga tacagaatat aatgaaaata tgaaagcaaa aagcggtaca
    20401 gaaccaaaaa cttacatccc tactctgaac gacgaattcg tcaaagcaat gggcgaagaa
    20461 gatggctcac ctgctaatgt agaagttgtt acaggtgcta cacacagctc acattcattc
    20521 atcatgtacg cacaacaatt agtgaatgca gctgaaaaag gcgacactca aacaatcgaa
    20581 gtagataata tcgtaacaga aaaataatca cataatcata ctaataaaag ttggagggtg
    20641 ggacgatagc cagattaggc agtcgtctca cctttcaaaa tgaagagagg aaaaagatgc
    20701 ggaaaaaact actattttcc ttaatcacac tgggcatctc ttttatggtg ctaactggct
    20761 gcagcaactc agcaaataaa acaaccactg aaagttcagc agctaccaag atattgaaag
    20821 atccatattc tgatgaacag ttcttgttag ggacttacgt tcgtatccgt gtgtatgatg
    20881 aaggcaaaga atcagcattg aaacctgcct tcgatcgagt aaaagaactt ggtgataaaa
    20941 tcacgatcaa tcaaaaaggt tctgagatcg atgaagtgaa tgagcaagct gggatcaaac
    21001 cagtaaaagt ttctgatgac gtctacacgt tagtcaagcg ggcctacgaa tatagccaag
    21061 attctcaagg cggattcgat atggcgattg gtgcaatcac acaattatgg cgaatcggtt
    21121 ttgatgacgc cagaaaacct tcccaagaag aaatcgatca ggcattgaaa ttagtggatt
    21181 atcataagat cgaactaaat gacaaagaaa aaactgttta tctaaaagaa aaaggaatga
    21241 tcatcgattt aggtgcaatc gccaaaggtt acattacgga cgaagtagtg aaagtcttga
    21301 aaaaacaagg agtgaccact gccatcgttg acttgggcgg taatgtctat gtcttaggcc
    21361 acagcccaag aggcgaaaac caagactgga cagtcggcat ccaagatccg aacatggctc
    21421 gcggatctgt tttaggttct attaaagaac ggaacaaaac attagttacc tctggaattt
    21481 acgagcgtta tttagaagta gatggcaaaa aataccatca tttgtttgat ccaaaaacgg
    21541 gttatccgtt cgacaatgat atcgcaagtg tcacgatcat aacagataaa tcaattgatg
    21601 gtgacgggtt gtctacagca gtcttttcaa tgggtgtgaa aaaaggactg gaatacgtcg
    21661 aaagcgaatt gaataacggg acggaagcga tcttcgtgac aaaagacgat aaagtatatg
    21721 tgaccgatcc aatcaaagac accttcaaat tatcggagga ctcccattac acgatgggtg
    21781 accgttctga actaaaataa ggagtacgaa ccatgacact aaaaatcttt ttagaagtag
    21841 ttgaaatacg aacaaaggtg gctagtgtat ttccttttgc ggtaggggta ttattctcta
    21901 tcgctttttt tcatgagata cattggctaa acactttttt attttttatt ggaatgctag
    21961 tttttgattt agcgactacc gctatcaata attacatgga ttatgaaaag gctcattctg
    22021 aagtatataa gtatgaagaa aatgtcattg gccgcagaaa tctatctcct gtattgattc
    22081 gaaacatgat tttagctatg attgcatttg tttttgttat cggcgttatt ctaacgatca
    22141 atacgggttg gcttttactt ttgatgggat ttatctgttg ttttattgga gtattctata
    22201 cattcggacc gattccttta tctcgaatgc ctttagggga aattttcagt ggggtgacta
    22261 tgggccttgg aatttttgcc atggtcgttt atgtcaatac attcggttca gatgtctatg
    22321 atctgacagt caacttttca tctggacaat ttgctttggt aggaaatctt tgggctatag
    22381 cagcaatcgt attagcttcc ttacccttgg tgtttacgat tgccaatatc atgttggcta
    22441 ataatttgcg ggatcttgac cgtgatattg aaaatcatcg ctatacacta gttttttata
    22501 tcggacgtcc tgcgggaaca gtcttattcc agctattgat gtatgcttgc tatttcgtga
    22561 ttttaatggg gttgttgagc ggtatctatg aatggccgat cttggtcacc ttcttgacct
    22621 tgccaattgt gaagaagcat ttggatcaat tcaagcaaac gttaccccag cctatcagtt
    22681 tcagctattc catcaaaaat atggtcttat tcaatgccag ttatgtattg gggctattac
    22741 taacaatcat tatgcaagcc atttaacagc atgaagaggt tgtgaaaaaa ctgccctgca
    22801 tcaagtaata agaacagcca aataaaaata gctccttata tttttcgttt ggttgggctt
    22861 attaccgcag atgcaagctt ttgaacaccg tttatca
//
LOCUS       DAYYGO010000039        22398 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000039 DAYYGO010000000
VERSION     DAYYGO010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.039"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1835)
                     /locus_tag="ACHA5T_001230"
     CDS             complement(<1..1835)
                     /locus_tag="ACHA5T_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFM8886816.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWR"
     gene            complement(1832..2536)
                     /locus_tag="ACHA5T_001231"
     CDS             complement(1832..2536)
                     /locus_tag="ACHA5T_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8886817.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(2548..4857)
                     /locus_tag="ACHA5T_001232"
     CDS             complement(2548..4857)
                     /locus_tag="ACHA5T_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFM8886818.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(5052..5516)
                     /locus_tag="ACHA5T_001233"
     CDS             complement(5052..5516)
                     /locus_tag="ACHA5T_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886819.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(5516..6142)
                     /locus_tag="ACHA5T_001234"
     CDS             complement(5516..6142)
                     /locus_tag="ACHA5T_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8886820.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(6149..6514)
                     /locus_tag="ACHA5T_001235"
     CDS             complement(6149..6514)
                     /locus_tag="ACHA5T_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFM8886821.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(6504..6842)
                     /locus_tag="ACHA5T_001236"
     CDS             complement(6504..6842)
                     /locus_tag="ACHA5T_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886822.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(6832..7158)
                     /locus_tag="ACHA5T_001237"
     CDS             complement(6832..7158)
                     /locus_tag="ACHA5T_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM8886823.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(7139..7417)
                     /locus_tag="ACHA5T_001238"
     CDS             complement(7139..7417)
                     /locus_tag="ACHA5T_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFM8886824.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETD"
     gene            complement(7419..8783)
                     /locus_tag="ACHA5T_001239"
     CDS             complement(7419..8783)
                     /locus_tag="ACHA5T_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM8886825.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(8796..9365)
                     /locus_tag="ACHA5T_001240"
     CDS             complement(8796..9365)
                     /locus_tag="ACHA5T_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFM8886826.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(9337..10524)
                     /locus_tag="ACHA5T_001241"
     CDS             complement(9337..10524)
                     /locus_tag="ACHA5T_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8886827.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(10588..12315)
                     /locus_tag="ACHA5T_001242"
     CDS             complement(10588..12315)
                     /locus_tag="ACHA5T_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFM8886828.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(12312..12764)
                     /locus_tag="ACHA5T_001243"
     CDS             complement(12312..12764)
                     /locus_tag="ACHA5T_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFM8886829.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(12876..13256)
                     /locus_tag="ACHA5T_001244"
     CDS             complement(12876..13256)
                     /locus_tag="ACHA5T_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFM8886830.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(13253..13639)
                     /locus_tag="ACHA5T_001245"
     CDS             complement(13253..13639)
                     /locus_tag="ACHA5T_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HFM8886831.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(13641..13919)
                     /locus_tag="ACHA5T_001246"
     CDS             complement(13641..13919)
                     /locus_tag="ACHA5T_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886832.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            13967..14695
                     /locus_tag="ACHA5T_001247"
     CDS             13967..14695
                     /locus_tag="ACHA5T_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HFM8886833.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            complement(14841..15317)
                     /locus_tag="ACHA5T_001248"
     CDS             complement(14841..15317)
                     /locus_tag="ACHA5T_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFM8886834.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(15277..15633)
                     /locus_tag="ACHA5T_001249"
     CDS             complement(15277..15633)
                     /locus_tag="ACHA5T_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886835.1"
                     /translation="MKKVNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVL
                     KIEQNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIR
                     GGVCEISLVKRLPRTR"
     gene            complement(15611..15796)
                     /locus_tag="ACHA5T_001250"
     CDS             complement(15611..15796)
                     /locus_tag="ACHA5T_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886836.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(15797..16069)
                     /locus_tag="ACHA5T_001251"
     CDS             complement(15797..16069)
                     /locus_tag="ACHA5T_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH/PUA domain-containing protein"
                     /protein_id="HFM8886837.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(16066..16266)
                     /locus_tag="ACHA5T_001252"
     CDS             complement(16066..16266)
                     /locus_tag="ACHA5T_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886838.1"
                     /translation="MKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDKY
                     LGEGFCGDSLFDDERILKEETE"
     gene            16394..16606
                     /locus_tag="ACHA5T_001253"
     CDS             16394..16606
                     /locus_tag="ACHA5T_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886839.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            complement(16639..16833)
                     /locus_tag="ACHA5T_001254"
     CDS             complement(16639..16833)
                     /locus_tag="ACHA5T_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886840.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(16846..17028)
                     /locus_tag="ACHA5T_001255"
     CDS             complement(16846..17028)
                     /locus_tag="ACHA5T_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886841.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            complement(17025..17165)
                     /locus_tag="ACHA5T_001256"
     CDS             complement(17025..17165)
                     /locus_tag="ACHA5T_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886842.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            complement(17655..18020)
                     /locus_tag="ACHA5T_001257"
     CDS             complement(17655..18020)
                     /locus_tag="ACHA5T_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886843.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            complement(18060..18362)
                     /locus_tag="ACHA5T_001258"
     CDS             complement(18060..18362)
                     /locus_tag="ACHA5T_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFM8886844.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            complement(18362..18661)
                     /locus_tag="ACHA5T_001259"
     CDS             complement(18362..18661)
                     /locus_tag="ACHA5T_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886845.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            complement(18654..18818)
                     /locus_tag="ACHA5T_001260"
     CDS             complement(18654..18818)
                     /locus_tag="ACHA5T_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886846.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            complement(18824..19093)
                     /locus_tag="ACHA5T_001261"
     CDS             complement(18824..19093)
                     /locus_tag="ACHA5T_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886847.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            complement(19105..19854)
                     /locus_tag="ACHA5T_001262"
     CDS             complement(19105..19854)
                     /locus_tag="ACHA5T_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8886848.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            complement(19851..20543)
                     /locus_tag="ACHA5T_001263"
     CDS             complement(19851..20543)
                     /locus_tag="ACHA5T_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFM8886849.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
     gene            complement(20549..21220)
                     /locus_tag="ACHA5T_001264"
     CDS             complement(20549..21220)
                     /locus_tag="ACHA5T_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFM8886850.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            complement(21213..21554)
                     /locus_tag="ACHA5T_001265"
     CDS             complement(21213..21554)
                     /locus_tag="ACHA5T_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886851.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            complement(21732..>22398)
                     /locus_tag="ACHA5T_001266"
     CDS             complement(21732..>22398)
                     /locus_tag="ACHA5T_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HFM8886852.1"
                     /translation="AVTTSLQVAESFEKKHQHVLRDIDALKKDVSNFGQMFVEGNEPD
                     SYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIRKNTVPQTIEDM
                     MIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGKESRAYEEISKK
                     VFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEIDQLNRQQRLFGD
                     DNE"
BASE COUNT         6185 a         4739 c         3484 g         7990 t
ORIGIN      
        1 attctccaag ctctcttttc tgcatctgtt tttaattcat caggataccc accaggtgtt
       61 tctcctccgc cagatggacc gccattttgt cctggtatca cttctttacc tctaacagta
      121 agttttccct gaatatctaa atcaccgaaa taatatgctt ttccgtttcc taataacaca
      181 aatccttttt ctgcagttgg agaaattaaa atgtattttc catcgccatt tgtacgtatt
      241 accaaagaat tttcctcaag aggtgtaggt gttacagcat ttggaaatgg atttcctgct
      301 gaatctgttg ttccgatcgt tccaatggat tcttttctat tccagaattc catacctttt
      361 ttggttagct ccatgatttt tttgttttca ttccaaattt gaagcgtgcc ttttacaagt
      421 tttaacacat caccataagc attaaaggaa gtttcgaata cttctgcatt tattgttcct
      481 actttgatga agtcagcaac aatttctcct tttgatgtga tagcaatacc gaacgggcca
      541 tttactccgt tgtctgaata acctaagcca tttaaattcc aacgccacac tcgctttgca
      601 ttcgccacat ttggagtatc catgataaga atctctgatg gagctttttc tggacgaaaa
      661 acgacatgcc caccagaatt accagtgatc catgccgtcg cattcaacac attttgaact
      721 aacgtttctg ttcgattgtc gattttcttt ttcaattctt gtgcttgagt atttactgcc
      781 gaagtgtaca gtgataaatc attacctaaa acaatatcct tatatttccc aagcgttggg
      841 aaataagtgt attcgaccat acgctctttt atctcgatat ctagctcttt cgccctaaca
      901 tgagcgacat caccaaagtg taaagacgct agctcttgat acatgtcacc gtattcgagt
      961 gtgtgttcca acgccaccat actgactgta tgagtagctt ttggctcatg gatacgatca
     1021 ttgtcaaata aggttttgcc ccatttgatc aattcatcaa ctgttttaca atctccgttt
     1081 tcacgtttgg caatacgtcg attctcgttg gttactccat caatttctaa ataaccatat
     1141 tcgataggtt ctttgtcttc atcataatcg ttatcaggaa caccgccaac caaatacaaa
     1201 ctattgacaa ttgattcttc atcaatttct tcttcgattg cttctaaatt gatgccaaaa
     1261 tcaatccgaa aaccattatc tgatccaatc tgttttacca gtttcaaatc atagttatcc
     1321 atatcaagtt ccgcgctagt cacgccagtc aaattttgat taccgttatt tgaaccaatg
     1381 attgcttcaa ctggcgcgac ttgtcttgct gtaaattggt gcgttgtccc tacattcgaa
     1441 agatagttaa atttttgatc aaatgccaaa ctgtttttaa gattggtcat gatctgagac
     1501 ccattgccgt tatccgtaaa cgacttgaca ataaagttct tattggccat aaaaccaata
     1561 tgccgagctg ttacagaaac ggaggtcaaa ttctttttga cattataaat ttcaaaatat
     1621 tgccatgatc catctggtac cttcgctttt ataaaatttc ctttttttaa ataagagcga
     1681 tactgtccgc ttcttgaata gttaccataa aaacgatatt ggccattcag cacacgattg
     1741 atttctggca aatcttccca atcaactaac gacgctccgt tcacgctcaa atcatctggc
     1801 atttttttat aagcataaat aaattcttgc gtcacagata cacactccta ttccaaaacc
     1861 gcacttcttt aaattttccg gatattttta cctgattcca ttcaggctgc aatactggcc
     1921 aatcaccacg cgtaaataaa ttcaaacctt cttgtattgc ttttccaagc tgagtatcaa
     1981 ccacgattgt tgcagctaac gtattaagta ttgttaggct tttatcaccg acagcgatcg
     2041 ttatatctcc tccgttcgat tctatttcca gatagggatg tgcaatttcg tcaccatgat
     2101 caaaaatatc cataacacta gaatgaaaaa ttttaggtgc ttcaccgatt tttcttttga
     2161 gtggctgaca acgaaaagta acatcgaatg tataaaagaa gccccactcg ttttcgaaag
     2221 ggacttcttt atccatgctg cagatagcct ctagatactt gtctggatca ttatgtgtga
     2281 ttaattggct tttaccagtg agccatcgtt tgacttctct cagtttcgaa tgtggaatag
     2341 tgatcccttc tatttccaaa tcaaaaggtt catagtcatc aaacgtctcc gtcaattctc
     2401 cgcttcggcc ctgaatcgtg taagtttcgt atcgcttgtt cggcataata tctggaagct
     2461 ctgcctcaat gatgcaattc atatcaatta ctgcattccg atttttccaa ataaaatttg
     2521 gttcatctgg attcataaat tgtcgactca aataggaaca cctcccaaat cacgaatagc
     2581 ctgtttgttt gctttagcaa atttacgatt catacgatct aactcagatg gattgtttgc
     2641 atcgactttg ccaatgtgta tatgctgttc aatgctacct ccagaaactc ttccactgat
     2701 ccctttgcgt ttttcttcgt ctgataatgg agtaactgtg gttttaccat tcttggcagt
     2761 cagtaactca ggtccagctt caccaacaat cgcttgtcca ttgattagat gaccgccttg
     2821 tgctaaataa ggcagttttg aaatagaaaa tgattttcca cctacaccag gaacccattt
     2881 tggtatcttt atattattca atccaccaat aaatccattg atcaatccaa tcatggcatt
     2941 aataggagct ttccctacag caacaatacc atcgaaaata cctccaaaaa tatcaactag
     3001 accttgccat gcttttgacc aattccctgt aaatactcca gtgataaaat ctagaaaacc
     3061 actgaaaatt cgttttccag cattataaaa attattgaaa tttgctataa caccatcgaa
     3121 aaatccacca aaataaccac ttaaaaaatt aaatatttca acagctacat ctgaaacacc
     3181 tttgaagaaa gaattcactc catcatgaaa ccatttaacg ttattatatg cccaaattag
     3241 tccagtaact aatgcgccaa tagctataac tgctaatgca aaccagcctc ccgacattcc
     3301 aaataaacca gcaagtcctt gccaaactcc tataaaactt ttaacttccc caacgatttt
     3361 tgtaacagat cccataagtg aaccaagaac gattagtact ggtccaactg ctgccgctat
     3421 accagcaatt gttatgatcc aattctttgt atcttgatct agagaccccc accatttaga
     3481 gaattcctgt aataaggaag ttgctttttc aaaaaaaggc aaaaggctta cttggactgc
     3541 ttcacctacg tcagccattg ctaattttgc attgttcata gcttgatcgg cttgatcaat
     3601 tgggtcgaga gtagcatcga atgtgtctcc tacagctccc ccactttctc cagcagtttt
     3661 tgctaaatct tccaggttca aagtacctct acgaattgca tcggccattc taggaccgcc
     3721 tttagtcccg aatacttctg ctgctgcatt gatggcttct gtttcagaac ctgcattttt
     3781 taccttgtct tgtagttctt ctagtccttg acttaaagat tttccgtctt tcgcataagc
     3841 cactgttgct ttagataaac tacttaaagc tgcgcttgaa tcaacaccag attgctcaaa
     3901 ttgtccaagt aaagtgaccc cttcaccaaa gctcaaccct aattgtttaa tttgtggtgc
     3961 gccatcaatt gctttttgca tcaagtcgtc tacagattga ccagtattct gcgatgtttt
     4021 cgttgttaca tcaaggacag aattcaaatc atcatattct agtccataag catcaatagc
     4081 ttgtcgagca gatattgctg attgcgaaac atctgtatca ttgatttcag cgtactgtag
     4141 gagataattg gttgaatctt ccagtttttt atccatgaat ccaaattgtg tatttacctc
     4201 accaatagct tccccaaccg tttgtaaagg taaatgagta tttgaaccaa cgtttttgaa
     4261 agactgtgaa agtctatcag cttgatcacc tgttgctccg gtttttgtaa tgatggtatc
     4321 aagtgcctcg tcaacttcac caaatgctgc aagtcctgct gctcctgccg ctacaatagg
     4381 cgcggttact ccaacagaca ttttttcacc aacgcctttt actttctcac ctgtttcttc
     4441 gattttctgg agtttttttg ctgtatcaac agaaacgtca ccttgttctt taagtgcatc
     4501 gttcgtctgt tctaatgctg tacgaagttt attttctccg gtttcagaat tgagtaattg
     4561 tttataaagt ttttctgatt cagcagaata ttcaccggtt tctttgacag atttttcata
     4621 ttcctctctc agcatttgag atcgttgttc agccaatcct aattgttttt ctaatttttg
     4681 tttagccgct cgcaattttt ctgtttgagt tgcatccttg tccatcgcag acacttggtt
     4741 tttgtactcg gcagcggcta agttcatttc tttgttgatt tctttgattg ttttcgaata
     4801 actgacttcg ccattcgttt taaaattaag aacgacatca gattctttac cagccattta
     4861 tcttagcgct cctttctacc accaaggcga tttatccata gttacagatt gaggtggttc
     4921 aaaatcggtg ttttgttgta accactgtaa ataagatttg agccacaagt taggtgtaga
     4981 ctttaaaaag aaactctcac tccaattcaa tagagtgaga gcaacgtaaa tataaaaact
     5041 ccaaggagtt cctatctctt ccgattcttt ttgtttttct ttctttttgc ttgcgctttt
     5101 tgaaagtctt gtggcttctt ggattttttt aggtcttcca cctgaaatgt ctggctagca
     5161 aaaatttcca tacaggtact ataagcagac aacacctctc cactcattcc caaaaatttg
     5221 aaaatagttt ctggatcttc ctctaaacca ccagtacgca gcatggcata aatcaaggca
     5281 cgcatgattt ttagatcact aggcgataag ttagcagaag aaatttgtcc ttctttttta
     5341 gatagcatgg cgttcatatc agattcaaat tgcgaataat ctccaccata cacatctgct
     5401 ataaattcca tcgtttgcat agtaaatgaa atagggaatt ctgcgccttg aataattaca
     5461 acagcagaat tttttaagtc ttcaacgtga attccataat cagataaccg agccattaac
     5521 ccgcacctcc aactttagcc aatgttttcc actgttcttc atcgtataca ggttgagcaa
     5581 tgaatttatt aaataattct agagaagctc cgtctctatt tgaatcaaaa cttgcataca
     5641 tcacattatt gtatttcagt ccgatggaaa ccagatttgc agttacatcg tcaatttttg
     5701 tttcttcctc tgcagtggca tactcttcgt caatcacatt ggatagttgt gtattaggat
     5761 accaaactgc ttttttaccg ccattttcaa tatttccaat aaatccaaag gcgaagtacg
     5821 gaaattctcg tgcggtattt ttgctaaacg ttactccgct tttagcaact aatcctttga
     5881 tttcatccat cacttctatc ggaataccca cgtgatccaa cgcaagttca tgttttgttt
     5941 ctcgcgacac acgacgaaac attttgctgg atgcccattt ttctaatgct gttccattcc
     6001 ctttgacacc gatttttgtg gcaattggca atcgtaaaac ttcactataa gtcggtgcag
     6061 ttccaacaga atctggcgtt gccatcatcg caattaaaat gtcgtccaat ccttcaaaat
     6121 aaaaagtatc ttgttttccc aattaaaatc acccttccca taaatcaagt atttttctag
     6181 tcataatttc ttcaattttt tctttgtttt gttcatacgt accacttgca aaatgttggg
     6241 cacgttgatt tgttgtgcca ttttcggcaa agcgccagta aaaggccgtc tcttcaaatt
     6301 gaactttcac ttgatcgttt tcaactacta cttttatttg atcgcgcata tgtttctttt
     6361 ttaagagcga cttaggaata ttaggaagca actgctttag ataaaagtta gctgcttctt
     6421 ctaaagattc caacgataat tttttcggat caacttttga aaggtttcct aaataatccg
     6481 atatttcaga aaaaccattc ttattacttg gcattttcca cacacctcac atatgtgtaa
     6541 tagttggtta cggtatcgtc gttctcatca ccttgtatac ctgtaaaatc tgaatatgga
     6601 atgccagcat tttgcagcgc ttgttcaata acaactaaat cctgttctgt tccaagtgta
     6661 aaaaaagaga cttggtaata aggtaatttt ttatagactt taccggatgc catttttttg
     6721 ctgttactca catttgagta cacaatgtat ggatacatcg tccctaatct ggctttgtct
     6781 ctgaacactg ctaactttgt tgatttcagc gctgttttca attcatcaaa gctaatcgac
     6841 ataagctaaa ctcaactcca tttctcttgc atcgggattc gtataaatgc gagtaatgtt
     6901 atacgttaca gaatcaattt tgagcgcact taatttctct gtgatggatt tatcccatct
     6961 gactttaatt cgtctgacaa cgtctgtctt ggcttgctgt gataaatatt tttcttgaga
     7021 agtcacaccg agttcttcat agaatattag acgcttaaat tcgtaaattg tagttggacg
     7081 atcgtttccg tctgttcctg ttttgatgtc tagcaattcg gctttccatc tgagattatt
     7141 agtctgtctc ttcggcattt tgaatcgctc cttgcacgat aaatggcgtc atggcattca
     7201 tagctttgtc gagttcatcc tctgaaactc tatattcata ggcaatgccg gcaaccatca
     7261 aaataagata ttcttgttgg ccaccagttg ctgttttgac ataatctttt gccatattta
     7321 aataaaaaga gagcaaagaa tcatccatgc cctcttcaaa atgaatatgt gatttgaatt
     7381 tttcctctaa agacaattct ttagtttgct cttccatctt aaccaccaac tggttttgta
     7441 atttcgtagc gatatactgc cggttcaaat ggagaataaa ccaattgacc atctagcaag
     7501 ttgtaaattt ggaatccaat ttgattttta ccagagaatt tttcaacaag tttttgaatt
     7561 tccaaggcac caataacttc ttgaatttta aatgcagaaa aatcgccaaa atataaaact
     7621 ggcgtgtctg gttcaccctt tttatctgct gcatctgtcc aatccacagg atagccaact
     7681 aattggtaac caattccacc ttctgcttgt gtgaatggcc gcaacaaagg aaatccatca
     7741 tctgttttca ttttttcaat ggcagtcaaa gcagctcgat taataataaa gcgtcccttt
     7801 ttcatcactt ctgtcactgg tgtattttta aattcgatta atgcatcata taatttttgc
     7861 ccagcacctg aagcagttag atctaaaggt tttttaaatg ctacagcctt tttggctaat
     7921 gcaccaggat tttcatttcc agcgtcatca ccattgaaca tataattgat ttctttacgc
     7981 acataagctt ttttcaattc ttccacaaca atatcttcaa ctggaacacc agacattttt
     8041 aataattttt tagttactgt tgccaaagca tcgaattcgg caggattaag caaaatttca
     8101 tcaaactgaa tagctgtttc agcaatatca gttgaacgct ctttcttgtt tacattcgca
     8161 tctgctttct tcacaagaat tggatatttg acatctcctg atgttcgcac cactgttccg
     8221 tatttacgaa gtaaattttc ttcttgagca taagtaataa cttcagatgc aattacttct
     8281 gggacagtaa ctgaaccgtt gccagcttca atccctaaag ctcgagcttc tgcttcagaa
     8341 atatttccag ctacaaaatt agcaaatgct tttcgtagtt gttgatcttt ttgtttcttg
     8401 gtcatttttg cacgtgcctc caatccgttc ttaattgatc caagtaatcc atctcgttgc
     8461 tgttgagtaa tcattccaga acgattttct gaactatctt cactgtctga atcttcttct
     8521 tgatcgttgt cttcaccaga acggctttcg tctgaatccg tactattcga ttgatcatct
     8581 gtattgtcgt ctgtttcatc agttccagaa tctgcgccta attcgtcttt aattccgttc
     8641 aattcatcaa taacactgtc aatttcttct ttcacggctt ctaaatctgc ttcgcgtaat
     8701 tctcctgatt caactttttc acgtaattca gtcaatcgtt gctcacgacg tgccttcatt
     8761 tttttcaata attctttatc catgtatttt tcctcctacg cttctagcgc ttgattaatt
     8821 tttttgatta atttttttct ttcttcgacg tttttttcta attcatcacg acttcttaat
     8881 gcagcttctg tatcttcgta tgctggcaaa ggcacaatag aaacctcgta taattccact
     8941 tcatggattg ttcgaagcat tggttcagaa ttgtagtccc acgtttcttc agtcggcaca
     9001 aaaccaaaac tacattggtt gatgtctcct cgttccatag atttaactaa gtcccttgct
     9061 accgttgtgt ccggtaaatc aacttcgaat ttcaaaccac gttcatcttc ttcaaggcga
     9121 agggttccgc ttttagtacg tcctagcaca ttggaccaat cgtgattgaa caaacaacga
     9181 acatccgaat tactaattgc ctttgagaat gcaccaggaa caatcacttc gcttaagtca
     9241 tcccataaaa gtgtttgact gttgaataca gcagcgtagc cgctaatggt cctcgtatga
     9301 gtttcttcat cggaacgtgt tgaaagattg gtgatgtcaa tcgtgcgaat ttccttcttc
     9361 ttcatttcca tcacctcctt tcaagtcttg atcattcgtt ggtaaggaat catctgttgc
     9421 atttttctgg ccaatcctag ataagtcatt tgaaatatag atagcttgtg tttctggagt
     9481 attctgttta ggaaaaccaa gcatttctgc cacgttatct ggacttgtaa tcccggtacg
     9541 aacgatgttg tagccaatat ttgtttttgt tgagtaagga acaaaatcca aaatattaat
     9601 tttccattcc actctatagc cagaattagg cataaaaaaa agagcggtgt aatgttcgct
     9661 cttgtttttc aatattggtt tgattgcttt gttgtgcaga tacatcatcg ctttttcaat
     9721 atctgtcttc atcagcgatt gatacgtatc aacattgatt cctagaaatt ttcctaagtc
     9781 ttttttgtaa acacctaaat aattcaaaat tgccgcgtca tcaacaggac tttttaatgt
     9841 ctcgatggaa tatccttttc ccaaaggaat catcttaaca gaatgatcac tctcattttg
     9901 cgttccttcc agttgatcca atatagcttt gacaattttt gtttgggcgc tattatttgg
     9961 attgatgtgg gcgtctagtt taagcatgaa cgcaagtaaa ccgcctttag tatatttgtc
    10021 cgtcaaaact ttttcagcgc tcagaacgcc ttctagagtg tttcttgcaa gatcaattat
    10081 tccagcacct tttaatgaat cagttccgat gttcttaatg tgtcgaatca tttgaccagg
    10141 tattttttga ccattcattt caaattcttc ttgaagtcgt tcattgattt tagttgttac
    10201 accgtacgcc aaatgaagct gatccctatc cgttaatggg aatgtctcac cattgatcaa
    10261 taaagtattt gtttccaatt tggtaaattc gaatcctgtc aaataatcat tgggactctt
    10321 caaaatattt agtaagtgat gatctttgac ttcgttgccg tcgggaccaa taacaattgg
    10381 tgtggccaat gccacctgat tagatatgtc ttgaactaat tcataaacat cagaagattc
    10441 catgatagat gaatccgtca catatctttg accataacgt gtgtagtgtc caaaaatatc
    10501 ctcgatatat ccacgttttt ccataaaaga atagactgca ttcgataacc gatcacgtaa
    10561 tttcaatttt tttcaccgcc tttctattta tctatagatg gaacttaggt aatcatctaa
    10621 ctcatctgaa tcaatatctg tcatttgatt catcgtttcc ttatgaccac acaaaaacgc
    10681 cacgaaccca tcaatctttt tctttgattg acgtttactt ggcgcttttt gtccattgat
    10741 gttagtaatt gctacaacgt tcaaagtgca ataaaggaat aatggattat caaattgaat
    10801 tcgtttctca taaaacaacc gttcgacatc gtcaaaagga gcattcaata ctttaggata
    10861 ttgagcaact tcaacgcatt ccaatcctaa gttttctaat ttctcaacaa gtttgtcact
    10921 catcgctgga tcataattca cttgctgaat atcatataaa ttcatgcagt cctctatgta
    10981 ctgcaaaatc tgatcttgat caatcatttt tccgtcgcaa aattcaacga aaccttgttc
    11041 agccaaatca ctgtaaggaa cattatcttc tttttctcga aactctaaat cttcattcgg
    11101 aataaaataa agttgcttta ctttaagaac cgctttacca tcttcatccc atgttgggaa
    11161 gtttaaagat acacaggtca aatctcgtgt acgtgataaa tctaaaccga tataacatgg
    11221 ttcgccactt aaatcaccaa gttcttgtgt ggtaactaaa catggctcta cttgatcctg
    11281 ttcaaagaaa ttatccgcgc cgttcacaaa tacatccaag tgtttcgtta gaaattcagc
    11341 tttggaatga gctgaccgtt gcgctgtttt aaatgccgat tctaaagcag ataaatcgac
    11401 tgatattccc cagttaggat tgcacatttc ccagactttt ctatccgtcc aatcataatt
    11461 tttatttggc tcatagatta gaacaaagtt tgaatcatta tcatcacgtt tcaacacttc
    11521 ttttgcttcg cgatatacac gcattccaac agacgacgaa cctttcccag cagttgagat
    11581 attaaacatc aatggctgtg gtaaagatat ctgagcagac ttaaaattgt catactgttc
    11641 cattttctcc tgtttatgca attcatcgtt caatacaaaa tatggattgg atccctcgat
    11701 attatcaata ttcttggttt gaacaataaa tttatttgag taagccatat cttcatgaat
    11761 atagtcataa gtaatactag aaacagttcc ctttggcccc ctaaatattt tagttccttc
    11821 aagtaggatt ggattgttta agattgtaac tgcaaaaggt ttggcagcat attgcgcttg
    11881 agcaaaatca gatgcgcaag catagcaatc aactgataac gccccttctc cgtacattgc
    11941 atatcctaat gaaccgacag caatcaatgt tttaccattc ttttttggaa tctggacata
    12001 agcctcacga gtaacgcgta caatttggcc tttttcattt tctttaaccc aaccataaat
    12061 ccaagaataa ataaattttt cccagggttc taaaagaaac ggttttccca ccatttctcc
    12121 ttttgtgtga acaataaatg attctaccca atccatcatt tcattcgcgc gatctacatc
    12181 aaaccaaata tcttttcttt ttttccactg ataccagcga tctattgcta aacgaacggt
    12241 cttaggatac ttcttaggtt tctttcttac ttcttttgca aataaatcag cataatttac
    12301 accaggttca atcatttttc agcacctgcc ttcttacgcc atttatttct gtgcgctgct
    12361 agttcatcta ctggctttac ttctgggcgt gtaatttctt catcttttct tgcggtcgat
    12421 cctccagcaa ttagtctacc agtttttgct ttgtttgtta atcctaataa atctagagct
    12481 ttcatcttct tatctgccca agtttctact tgttgtgcca atggatgttt tgattgatta
    12541 gtagctcctg atttattcgt gaatttttgc gtctccggaa atcctttttc cttccacaat
    12601 aaatatttgt gttggtaaat ttcaaaaata tccaagtatg attcgattaa tggatcaaga
    12661 gtgatggtgt acaaatcaga caaattcatt atttctaaga tacgagcttt ttcagcactt
    12721 actttttcat caataatcgc tttacgttgc gctttagtcg tcatttttgt atacaccccc
    12781 ctttgttttt tgaaaaattt gacctaacga tgcgcgtgac tccccgctac cctatctccc
    12841 cacgcgaaaa aattttgaaa atgaataggg gggcttcaat tgaaatacga aggaaaaact
    12901 tttttgtctt ccgtttcgtt ttcaacaatt ggatgacatt ttgaacataa tagcatgaga
    12961 ttgtttggat caagcttaag cagttcgttg tctttgattg gtacaatgtg atggacgtgt
    13021 gctctctttc caaatatgaa ctgaccacac cgctgacaat gaccaccttc tctttcataa
    13081 ataaattgac gcatatcttt ccatgcttgc gttcgataga atggtttgtt ctcatgatga
    13141 taaacagact ttgcttgctg cttcttcttg cgtgatctgc ttgatctctt gtgttcagta
    13201 cagtagatac cctttgctat tttgttcgtg catccgtcaa actgacagta tttcattcta
    13261 cttcacgaat aagatttatg atatcgcctt ttgcacggat agcaccagga atatcaatac
    13321 catgtttctt agcatatgca cgcaattctt ttgcagtcat attgtccagt tcatccgtat
    13381 cttcagtgga ctgatcatta gtagcttcgt ctccatcaaa attagcagca gtgtttccat
    13441 cgctatcaag aatttcactg ctatgagcta tgagttcgcc attgacagca acaaatgatt
    13501 tgccatcact caaaattcct ttttcttctt ttacttcaaa atttggttct tgatctttcg
    13561 gaacaactac tgtccttttc ttttctgaat cccaatactc tgttccacta atggatgttc
    13621 taattttgat cattgccatt ttaattctct cctttgtaat ttgtacggat tacttttgcg
    13681 cccattcgct cataccattc aatcgtttcc tgcagattgg gtagagtgtg tgacaataaa
    13741 gaaataacta acgtattcat tccagttctg taatcatcat tctccacttg cacatgtgca
    13801 tgattaccat tccaattttc tctgtacgga ctattctctg cttcaattgc tttcttataa
    13861 actttagcca aacttgattc tacttcaata ctaagtaccg cttcatgaaa atcattcatt
    13921 tgtttactct cctatcagat attttgtggt atgatttata aaaaagatgc gaggtgtttt
    13981 aatgcttcaa gaagttaata ttaagtatcg tgatcgtcaa aatacagaga caatttattt
    14041 gccaaatagt tgtccgcatt gcggaaatat aatgactccg tatgtgtatt ttggggtttc
    14101 atcatatagt gcgttcgata atgatagaat ctttggcgta ttagtacagt gtataaatga
    14161 tgagtgcaac aaattttata gtttacaatt tataaatacg tttaatgaag atgaaggata
    14221 ttctgaaaat attttaatta aagattatta ccgtccgccg attaaaatag atttaccaga
    14281 aaatattgaa aaagtttctc ctacatttgt tgatatttat actcaagcca cacaagctaa
    14341 atctgagcaa ttggaacaaa ttgctggagt tggttataga aaagctctag aatttctaat
    14401 taaagattat gcaatatcta aaaattcaga ggatgaagat aaaataaaaa aaatgttatt
    14461 aggtaaaaca attgaaaatt atttgaatga ttttcctaaa ctccaaagat tagccaaagc
    14521 tgctacttgg attggtaatg atgaaacgca ctatgttcgt agacatacta ataaagatgt
    14581 tcatgatatg gatgcattta ttaaatcagc agcgcaattt atcgcggctg attatgatgc
    14641 tgatttagct gatcaattta taaatggaaa taactttatg ccaactacta attaaaagaa
    14701 taaaagagcc acagatacca attaataaaa cggttgggat atcttggctc tttcatattt
    14761 ttttgacact aatagaatat catgctgcta acaatgttta catagctaca atgaccgaac
    14821 atttagtgaa cagtgtaatt ttagaataaa gagagttgtt ctttgtactt ctcgctattg
    14881 taaaaatcaa tttcgttttc ttttttcaat cggtcagctt gtctgtgttc atactcatct
    14941 aaaaagttca atgtctttct aatttctgca tgtcgctgtc tgacatacga tgagctatac
    15001 ccaacagatt cagcaatatc ttctaagctc atttgatcaa catatttcat cttaacaatt
    15061 tgattatcga taccagaaaa gctatctatc agcaataaca tttcttcttt ttgctcgatc
    15121 aacaattcca attcattttc tatcttttgg atattctctt ctaaagaaga agttctcgaa
    15181 ttcttttcga tgcggacgtt tgctaaatca ccacagaccc atcgattcaa ttcaagctta
    15241 cttttattaa gattccactt taagtaaaga atctgctcat ctagttcttg gtaatctttt
    15301 aaccaatgaa atctcacaaa cgccacccct tatggtagaa tagtattgcg ctgctatctc
    15361 gaaaggggta gcttttttat tttttcttag ccgttccatt ctggattgcc ttttgttcca
    15421 aacgtcgttt tttcttctta atctttgatt ttgtttttcc catacaattc tcctttccga
    15481 aaacacattt tgttcgattt ttaatactaa ataaaagttg gccagaattt ttcgttcgtc
    15541 tttactcatc tttttttgaa taagattact agcataaaaa tagttagtac cacgaaaatg
    15601 aacaggtaaa tcaattacgt tcaccttctt catgccagtt atcagccact agaatctttg
    15661 cgttagtgcc gccaccgttt tcttttttgt atttttcaaa tgctttataa gcagctgttt
    15721 ttgatttata gatttttccg gaaccgactg ggaacatcca tgtgccctct gtgatagcta
    15781 agatgtatac tttcatttaa ttcacccctt taatccccaa cactacatat ccggacttct
    15841 gcatgtaatc agtaatataa gtgatctctg ctttgtatga gtcaccagtg taactaaatg
    15901 ttggtctcgt tagtccaggt aattcatgtt taatgtattt tttatattca cgtagtatta
    15961 actgatcacc aactttgaag tcacgatcat tcttgcggat ctcgaattgt ttgcgtccgc
    16021 tcgtcactgc ttcaaaatat tctggtagta tttttagttc gtggatcatt ctgtttcctc
    16081 cttcaaaatt cgctcatcat caaaaagtga atctccacaa aatccttctc ccaaatattt
    16141 atcgttcatg tataagtcaa ccaaatattt gtgatctgct tctcgatatt cattaatatt
    16201 aacgatcttt attttgtaaa cattaccttc gtcgctcact atcttgaatt gttctctgac
    16261 tttcaccacc gttcctcctc tttttgaaaa aattttagtg tttttgtaag accccctaaa
    16321 tcagcttttt tatttgatat aatacttgta tcgattaatc aaatcgaaat taaatttaaa
    16381 aaggtgattt atcatgaata tcaacaattt agtaaaactc gttgaacaac gttttaaaga
    16441 atcaggatta gaacaattaa aaaaagataa ttctccagaa ttattttcta attttaccga
    16501 agacgacatt cctactctta aacaattaaa atattattct gattattgga cgcaaactcg
    16561 tgagaatgaa ttaaatttag aaaaactcat tattaaaaag aaatagcaac tccagcccac
    16621 acggctggtt ttttagtttc aacttccctc cttacgggtc aaaatcatcg gttccggcat
    16681 aatctgatca tcctttagca acaaatcagg atattttttc cttagcttct tattgttcgt
    16741 gtaagttttc aatagccatt ggttggcttc gcttttgctt tctccagaaa aataaatttt
    16801 ccctgtttgg atgcctgcaa gttgaatcat cattttcacc tcaaatcaaa ttttttctgc
    16861 tagatggagc aaaagcaatc cgacatcaag aatcttttga cccattattt cagctacttt
    16921 gctcggctta actccttgag aaaagagcaa gcgtgcttgc tctatctctt cgtgagtcca
    16981 aataaatttg gcttcctcaa ggataattac tggatcagtt gctctcattg ttcctctgga
    17041 aaaatgatgt cattcttatg cttggaccag gtatctgcat atggaacaaa gtattgtcta
    17101 gctaattcga tttgatcaat taaagcagtt tctgataatt catggtctgc agcaatttca
    17161 ttcatactat cgccttcatc taaacagata agaccgttga cgttatcgat ttttacattt
    17221 gtgactgctt gactttctcc acgctcataa tattctacag ttgatactga tacatcagct
    17281 tcggagttta attgcagaat ctgttactgc cgggataatt taatattctt ttcataattc
    17341 accttttttc ctactgacat cgaatatttt tcatgttatg attagccgtg aaaggagagc
    17401 tatattatgt gtttacacat gatgtcctta gctcattcgg ttctttggaa gcttttttgg
    17461 aaataactct caggttcttg acgcaaatct catttattct ttgaaacaaa aaacttccat
    17521 tttccgagag ctttggatca cttcaaaaga gatttgcatt tatatttctg tgaccttgag
    17581 tcgcgtaaag aaccttacag caatggcggc aattccactt tcaggaaacc gccttttttc
    17641 attgatattg gtggttagcg gactacccat tttctttcgg cagtgccaaa ccacaaatga
    17701 tctgtgttga tgtttaggta gttgtatacc agataagcta aatccaaagt gcctgcatct
    17761 tcaagacgac aatattccaa aaattctccc atagctggac ataaaaatcc tggagctatc
    17821 atatcccaac agttaatagt gcaaagaaat tcgtttagct tctgaacgtt ttctttggtt
    17881 tcacgaatta caacatactc caattcttct ccgctggcag agcattccaa aacttcaaat
    17941 tgattaagaa atggtagaac atatgcttca aacatcaaca ttccatcttt ctgtttttgc
    18001 acttgttttg catcaatcat gtctatcctc ctattcagtt atttcttcca ttaatggaat
    18061 tatttcaggt cactggactt aacgaaaaca ccattgacca tctttccagt ccgacctttg
    18121 atttcgtcat aagcaaaatc caagcactcg tataaatcca tatcattttg cattgccaag
    18181 atgatcaagg ttactactac atcgccaatt ccgtctctta aaccatgttc atcttttcta
    18241 gctagagaag cggcaacttc cccgatttct tcgaccgttt ttaacatttg cttgctgaaa
    18301 tccgcttgat ccaatccctt atcttttgcc cactgctcta ctttcgcgat tagttcgtcc
    18361 attatttctc ctccacatac ctaaactgtc ttccttttga atcaatccat aagctcctag
    18421 ctctatccca aataatattt ttgctcagac cagtaatttc agataactgt ttagcggtac
    18481 ccgttactag aattcgatca ccatgccaga ttgcaatctt tctcggcgtt ctccgtttgg
    18541 gcttttcagt ccacattgat ttaccgagct tttggacttc tgcaactatt tttttatctt
    18601 cctgccaatc ttctgaataa gtcaattcga taattcgctg cattgccgct ttcttatcca
    18661 tcccgacatt ctcctttcag taatttgagt acttgatcaa gtgcgctctc acgtccgcca
    18721 tgaaatgtgt tgagccactt gtcttcgtac gacacacttt gtcttaaagc ttcttgatgc
    18781 attaattcga tctgtgctgt aaatgtcttt aggtccatct tgtttacacc tgctcaagtt
    18841 cattaagatg tttttgcaag cctttaacac aatcaatgaa tagtaatttg gtatatgcta
    18901 aatttcttaa ttgcgttcca tcgatataga gtgcgaaata gtatctgagt ttactccaac
    18961 tcgaacgatc attcttaatt ccttcaattc cagcttcttc gagttgatca tatacgtctc
    19021 tcaggacgtc taattcctcg ccctttttat aattcgctat ttcattaatt agttctagat
    19081 aatcgatctt catttttcca cctcttagaa tggtgctttt gattgtctat tagctcgttc
    19141 tagcgctttt ttcttgagat aggcttcttg gtcgattgcc cattcaggaa gcttctctcg
    19201 tcttcctgtg cgcttgtatc cactgcttgc gttcttaggt tcactttttt ctttccttgc
    19261 ccaacttcga atagttgcca aatagttttt ataagtctta ccagatgatt cacaatactc
    19321 tgacagtcgt tcgattcgct cttggtaatc attagggaat tctgttttga gtttctccat
    19381 ctgctcatct gacaaaagaa catttttata ctctccgtat ttatgacgga cgggcttagc
    19441 cttcgatttt ttcgaaggcg gtaactctct tatatattct tttgtattat taaatgtatt
    19501 attaatagat gtattattat ctttgacttt ttcgtcaata ggggtattgc gtttttcgtc
    19561 aatagggtat tgattaattc gtaggtacct attgattatt tgattggtac cctctttgta
    19621 aatgatttcc cgattcaagt atccaaactt aatcaaatca cttacccatc gcgatatggt
    19681 ctctttattc acaccatata aatctgcaaa gtactcattg cctgcccaac aaaagcctct
    19741 ttcattacac aaggccgtta tctctccgta taacaactta gtatttggtt taagtctttt
    19801 gtcgtacctt acgttggctg gtataatcgc ataataactt cgatgttctg tcatttttcc
    19861 cctccaatat ttaacttttt gattgttttc tggtttaatt tgatcccttt gatttgatat
    19921 ttatttttga aattaatcac acctattttg tgcttctctg tgtgatggat tctgcagagt
    19981 gctgcaaatg tgtactctgc atgatcaact tctttgcgct ttcgtcttcc tagcgctttg
    20041 tcaaagtgat cgatgtcagc tcctgttttg ccacagatgc agcagactct ttttgtgatg
    20101 catttgtaga agtaatattc ttgattcgca ggtaaaatct catagccttc tttgaaagga
    20161 atatgatgtt caaagatgaa atctaagatg atatttgcta agacattggc atcactcaca
    20221 gttgtattcg attcgtcttt caggcttatt ttgcgccctg tgacaccttc aaaacgaaag
    20281 tagaagaatt ccttccagaa gtccgttggc atgcctgtat cgataaaaat atcgcctatc
    20341 agcgcataga tgaagtttcg ttgctgtacg gtgaaacgtc taggatcaat aaaacgaatt
    20401 tcaatgactc gatcaccatc gtagccgtca tacatcgtct ttagtcgatc aatgttcact
    20461 tcctcattga tggttgcgct tatgtctttt cctttgaact ttttcagaac cgctgaatat
    20521 gaatcgatta atggtttaaa cactcatatc acttcttatc taattctttt ctcttagctg
    20581 ctattgctcg ctccatcaag gcacattgct catagcttaa ctgttcaata gtttcaacgt
    20641 tatcagctaa gagccctaat ttatctgtct gctcattaac atattcgatt aaggttttgg
    20701 tcatatcttt acccatctgc tcattgaaag cttctagaat cgtctctagc atgtttaatt
    20761 tctttgtatc gattctaggt ggtgttggaa tatcttcccc ttgaaataca tataatccca
    20821 gtccgtgtag agccaatgct ttcacaaagc atcgcttcaa tgagttattg atttgcattg
    20881 catttggttt aacaactggt tggtttcgat aatctaaaac aggaaataat tcggtttccg
    20941 tgtgtccttt aaccgttact gagacagata cataagtccc agtttcatcc ataagaaaag
    21001 gtttatattc ctcaacaaga aagtcttgat gagttccaga aacaaccctg tagtgtttgt
    21061 actcattaat agttaccgtt gcctgtggat cattcttttt cataatctcc cacgcgtgag
    21121 cccaagataa ataatcaaaa tttccttttt tcttgagaat tttatttaac ttacgactaa
    21181 aaagtttttc aaagttcgtt gtccctttga tttcactcat caaattctgc ctccatttca
    21241 gcaatgtatt tcttacctgg tccgtaataa gagatatcaa tcaagttatc tctgtcgtac
    21301 tcttctagcg catcaatcaa gccatcttcg atgacgtaaa tatattcagg tttattcgaa
    21361 tgcttcgata gatggataag ataaacatga tcccaaatag ttacatggtt gcctaaatca
    21421 tcttgatccc aagctagttc ttcattcgtc aaaagatttc gtctgatttt tcgaccactt
    21481 gtttcctcaa ttttcggctt gccccaatca ggatcaatca aatattgatc tagagtggaa
    21541 agttcttttt ccatatgcta aaatctcctt atgatgtgtt ttctttgtga ctctttgctt
    21601 gccggcggag tcactttttt atttgttgcc atgctttttg cttgtcaata tgttgttggc
    21661 ttaggatgat tggtttatag tatttccacc agcaattagc aattgccgtc cctattctta
    21721 gcgcttcagc tctattcatt gtcatcacca aaaagtctct gttgtctgtt cagttgatca
    21781 atttccatac ggatcgcagt ttctggcaac cacatttcaa taaatgaaac agcatcatcg
    21841 aatctcttac gaggtaactc gccatatctt gggattgaaa aggtacgttt aaattcagac
    21901 caaaattttg agaatacttt tttgctgatt tcttcataag ctcggctttc ttttccccct
    21961 agaactccca taactttcat atttcctttt tgcttaattt caaactcttg ttgtccgcta
    22021 attcgcatag tatctttaag catggaaaca tcttttttaa catctttcat ttcttctaat
    22081 tgatagatca tcatatcttc aattgtttga ggaacagtat tcttgcgaat aacatcttcc
    22141 atttcgttga atgcttcaat gtatttttgt ttgaagtaga tagctttctt tcctgtaaac
    22201 cccatagcca gcaagaaaaa tccatctcta ctaatgaaga aaactcgtcg atttctgcca
    22261 tatgaatctg gttcattacc ttctacaaac atctgtccaa aattggacac atcttttttt
    22321 agtgcatcaa tatctcttaa aacatgttga tgttttttct cgaagctttc tgccacttgt
    22381 aagctcgtag tcacagct
//
LOCUS       DAYYGO010000040        22270 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000040 DAYYGO010000000
VERSION     DAYYGO010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.040"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(103..252)
                     /locus_tag="ACHA5T_001267"
     CDS             complement(103..252)
                     /locus_tag="ACHA5T_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886853.1"
                     /translation="MKKKVIPYCPKPRNAFERGLFKYFEGKKGQDHKEENQKKKSKKR
                     SEYIF"
     gene            1220..1546
                     /locus_tag="ACHA5T_001268"
     CDS             1220..1546
                     /locus_tag="ACHA5T_001268"
                     /inference="COORDINATES: protein motif:HMM:NF013698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8886854.1"
                     /translation="MFKIFRKNCCGLDVHKTWIYACIGITDANGRTVYKQARFSSFTK
                     GLNELAEWLAKYNCKDVCLESSGKFWIPVFNILEKTCFVTLAHPKYTKPQKGNKTDRK
                     DAKMDL"
     gene            1554..2462
                     /locus_tag="ACHA5T_001269"
     CDS             1554..2462
                     /locus_tag="ACHA5T_001269"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFM8886855.1"
                     /translation="MCDMIKPSFIPGLEIRQLRDLMRYRTKLTNMKTSEKNRALNCLT
                     VSNLKLDDVFSDVFGKSSTSIIQYLLEHPGESFDVRPFIHARCKTPVEEIQAAVEGAI
                     SVPQAIKLRQCLNHINEIEKHREEINLEIQALAEPFSPVLELLRTIPGLDTQPITAIA
                     ILSEIGPDMSVFPTSKHFISWAGCCPRNDRSARRTKSTRISRAGQSLKPLLVQIANAL
                     LRSKEHPEFKNRYRRIKANRGHKKAIIAICKMLLTAIWNVLSKVEPYSAKGYLEPKPA
                     KEEKVLTQSQALDLLRKRGYTIIDSA"
     gene            3088..3342
                     /locus_tag="ACHA5T_001270"
     CDS             3088..3342
                     /locus_tag="ACHA5T_001270"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HFM8886856.1"
                     /translation="MIKSNQKSGFYTFIKAISSVLECDCIIGPYSMLIKMGFPSTESL
                     DSFINDLSKFGRTNTQIVFSTLVSLMVYQFWNVYHALLLK"
     gene            3884..4042
                     /locus_tag="ACHA5T_001271"
     CDS             3884..4042
                     /locus_tag="ACHA5T_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8886857.1"
                     /translation="MNKKQACEFLGIDNNTLDKWIRVYNFPVIKIDGVVRFDVEDIKD
                     FMNTFKKN"
     gene            4114..5340
                     /locus_tag="ACHA5T_001272"
     CDS             4114..5340
                     /locus_tag="ACHA5T_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8886858.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGIASEKYQKVFPTRQLAIKA
                     EREMKKKIETVLREENANSLELKGKIKFKDFYESKWLPRYELGQTTRSNRAPSNVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILCYIEFNRTTKIIQCITAPKKMALEEKRIREGNQALSSEELTDWIEAVNNDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFNKNTVRLKYALDKYQRKTHTKGWKDTV
                     IQAPEVVMTLLREWKSAQAEQLLKLKIIQTPDQYLFTYSKPSGEVNCPVHADYLNYRI
                     NAIKKRHPELAQLSPHKLRHTYATIAREGGANMNQISNALTHSDISTTKIYVNTPDVV
                     DKSVFEAFQKGLNKCD"
     gene            complement(5429..5821)
                     /gene="rpsI"
                     /locus_tag="ACHA5T_001273"
     CDS             complement(5429..5821)
                     /gene="rpsI"
                     /locus_tag="ACHA5T_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFM8886859.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(5837..6265)
                     /gene="rplM"
                     /locus_tag="ACHA5T_001274"
     CDS             complement(5837..6265)
                     /gene="rplM"
                     /locus_tag="ACHA5T_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFM8886860.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLAGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(6799..7266)
                     /locus_tag="ACHA5T_001275"
     CDS             complement(6799..7266)
                     /locus_tag="ACHA5T_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFM8886861.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(7386..8924)
                     /locus_tag="ACHA5T_001276"
     CDS             complement(7386..8924)
                     /locus_tag="ACHA5T_001276"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFM8886862.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            9004..9690
                     /locus_tag="ACHA5T_001277"
     CDS             9004..9690
                     /locus_tag="ACHA5T_001277"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFM8886863.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(9891..10418)
                     /gene="msrA"
                     /locus_tag="ACHA5T_001278"
     CDS             complement(9891..10418)
                     /gene="msrA"
                     /locus_tag="ACHA5T_001278"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM8886864.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(10502..11179)
                     /gene="pgmB"
                     /locus_tag="ACHA5T_001279"
     CDS             complement(10502..11179)
                     /gene="pgmB"
                     /locus_tag="ACHA5T_001279"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFM8886865.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(11176..12304)
                     /locus_tag="ACHA5T_001280"
                     /pseudo
     CDS             complement(11176..12304)
                     /locus_tag="ACHA5T_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
     gene            complement(12304..14487)
                     /locus_tag="ACHA5T_001281"
     CDS             complement(12304..14487)
                     /locus_tag="ACHA5T_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFM8886866.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(14484..15509)
                     /locus_tag="ACHA5T_001282"
     CDS             complement(14484..15509)
                     /locus_tag="ACHA5T_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8886867.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTANRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(15554..16381)
                     /locus_tag="ACHA5T_001283"
     CDS             complement(15554..16381)
                     /locus_tag="ACHA5T_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM8886868.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(16378..17445)
                     /locus_tag="ACHA5T_001284"
     CDS             complement(16378..17445)
                     /locus_tag="ACHA5T_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFM8886869.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(17471..18307)
                     /locus_tag="ACHA5T_001285"
     CDS             complement(17471..18307)
                     /locus_tag="ACHA5T_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8886870.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(18304..19173)
                     /locus_tag="ACHA5T_001286"
     CDS             complement(18304..19173)
                     /locus_tag="ACHA5T_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8886871.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(19243..20526)
                     /locus_tag="ACHA5T_001287"
     CDS             complement(19243..20526)
                     /locus_tag="ACHA5T_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8886872.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(20540..22225)
                     /locus_tag="ACHA5T_001288"
     CDS             complement(20540..22225)
                     /locus_tag="ACHA5T_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFM8886873.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
BASE COUNT         6388 a         4666 c         4033 g         7183 t
ORIGIN      
        1 tagtcattct ttacaacgat ttatagaaaa acaacctctt taaatataaa aggtacgggt
       61 tactgattac ttgtaaatct ttacgttcca gttttattct ttctaaaaaa tatactcact
      121 tcttttctta gacttctttt tttgattctc ctctttatgg tcttgtcctt ttttgccttc
      181 aaaatattta aataaacccc tttcaaaagc atttctaggt tttggacaat aaggaatgac
      241 ttttttcttc atataaatac ctcatttaac tatttgatat catcacatta tcaaaaaaaa
      301 taggtttttt attaaaacta tcttgacctt tcgggagatt tcactgtgat ttatttttat
      361 tcccatactt ttttcgttgg tcactttcgt atacctaatc taagcgatca tcggcgacgg
      421 tgcttttaca acgaatggca ggacaaaaca aggggcggaa aaactttttt gcctatttca
      481 taaaaaactt tttcctacag caatcccttg ttttgtccta ccacacgttg tgaacccaaa
      541 gcacgccgac gaacgcttag ctatacgaaa atgacctcca aaaacaaaaa agtgaggtga
      601 atagaaacaa aaagaaattg aaatctccca aaatgtcgtc cgcctcggag acaacttgct
      661 gctgaaaatg gttggttaat tcatttaagt attaaataag ataaatcatc aaattaatcg
      721 gaaaaatagc atgattggcc ttatcaaaaa taaactgtaa aaacttatat ttctatgagt
      781 ggtggaacaa tgctaatacc aaattaattt atcgattacc cacctgcaca aaccaaagat
      841 agcattatta ggaaacgaat caatcagcga atccagcatt ataatgagga tactaaaaat
      901 aaagttttca taattataaa tccgccttcc tacgaaaggc accaatgagg tgatgaaaac
      961 cttagagcgc tttctttctt tttttattcc gtgttaccct ttccttgcag gtccctggta
     1021 cactacagaa cccgaggagg tgagtggcgg ggctgtatag cggcaacccc gcataattat
     1081 atggtgtcgg tcatccgtta aggcatcaat gaatggcgca aacccgtagc ccgtaaactt
     1141 atctcttccg aagttgactc gatttcgccg gatgaccagt ccctaccaaa cctgtactat
     1201 ttttttacag gaggttgcta tgttcaaaat ctttcgtaaa aattgttgtg gcttggatgt
     1261 ccacaaaacg tggatctatg cgtgtatcgg aatcacagat gcgaatggtc gtacagtgta
     1321 taagcaagct cgcttctctt cctttacaaa aggtctcaat gaattagctg aatggcttgc
     1381 taaatacaac tgtaaggatg tgtgtttgga atcttccggt aaattttgga ttcctgtttt
     1441 taatattctt gaaaaaactt gttttgtcac actggctcat ccgaagtaca ccaaacccca
     1501 gaaaggaaat aaaacagatc gcaaagatgc aaaaatggat ttgtgatctc tttatgtgcg
     1561 acatgatcaa acccagtttt attcctggtc ttgaaatccg ccagcttcgt gatctaatgc
     1621 gttatcggac gaaactgacc aacatgaaaa caagtgaaaa gaatcgtgct ttaaactgct
     1681 taaccgtttc taatctgaaa ctagatgatg tttttagtga tgtgttcgga aaatcatcca
     1741 cttctatcat ccaatacttg ttagaacatc ccggtgaatc atttgatgtt cgaccattta
     1801 tccatgcccg ttgtaaaact cccgttgaag aaatccaagc ggcagttgag ggggcgattt
     1861 ctgtacctca agcgattaag cttcgccaat gcctcaacca tattaacgag attgagaagc
     1921 atcgagaaga gatcaatcta gagattcaag ctcttgcaga acccttctcc cctgttttgg
     1981 aactacttcg taccataccg ggcttagaca ctcagccaat aacagcgatt gcgattcttt
     2041 cagaaatagg accggatatg tccgtgtttc caacttcgaa acactttatt tcttgggctg
     2101 gatgctgtcc aagaaacgat cgcagtgccc gacgaacaaa atcaacacgt atctctcgtg
     2161 cagggcagtc gttgaaaccg ctacttgtac agattgccaa tgctcttctc agatccaaag
     2221 aacatccaga atttaaaaat cgctatcgta gaatcaaagc gaatcgtgga cacaagaaag
     2281 ctatcattgc tatttgtaag atgctcctga ccgctatctg gaatgtctta tcaaaggtgg
     2341 aaccctattc tgctaaagga tatctggagc caaaacctgc gaaagaagag aaagttctga
     2401 cgcaatccca agctctggac cttttaagaa aacgcggata taccattata gatagtgctt
     2461 aaaccaaagt tacgttgatt cttcctaaat agactcgttg tcttccattt caaaaatttt
     2521 gaaaacggta ggcttattta gtgtaccgtt cttcaggtat cttcaaatag atttattttc
     2581 aaacttattt cctttcttct tttactctcc tatagcttag catttaagtg ggcactcgtg
     2641 tcaccttgct agatagctat gaatgccgta ataagcctcc tagtatatag gcagactgcc
     2701 aattataact gattcaatcc acattatgat aaaatccacc ttttcgtttc atatgtgttg
     2761 aaatatcaca tatgtcaaaa gtgcacgagt cttttcattc agtgctttgt aaatactcaa
     2821 aattattcct tttcacttta aaaaaaagat aattagtaca aaatcagaaa tgaggaaagt
     2881 attaggatgg actagaaagg agttgttgaa tttgttgcaa cagattggtc atttatcaat
     2941 taaaaattag gtgaagtttt attcatcact gcagctgctg tttcccagag aataaaacgt
     3001 ctagaagaaa ctggtcagtt actgtattat caagcagcaa ttgactacga acaagcttga
     3061 ttactaacca aagcgtttat ccacctgttg attaaatcaa atcaaaaatc aggtttttat
     3121 acgttcataa aagcaatctc cagtgtactc gaatgtgact gtatcatagg tccttattcg
     3181 atgttaatca aaatggggtt tccttctacc gaatcattgg acagttttat caatgattta
     3241 tcaaagtttg gacgaaccaa cacccaaatc gttttctcga ctcttgtttc cttaatggtt
     3301 tatcagttct ggaatgttta tcatgcgctt ttattaaaat agaaaagagc ttttttcatg
     3361 cgacaaagca gtgatcattt attggcacat gcaatctttg tggatccatt ttttgaaaat
     3421 acgatattta gttttgaccc aaaaatcttt aaatacgtat ttgataattt atcttaacaa
     3481 ccagataatt ttattcagca ccgtattatc tatattgaat tctcggaatc aagataaatt
     3541 tttttccgtt tttgtttctt ttttaatctt cttcttgtta ttaatagtga ttaggatgat
     3601 attcctatat tgatctttga taattagata taatttttga ataacgttcc ctgctattcg
     3661 agctagaaat acaagtacgc catgacctag ttagcgacaa ctacttagag atttcttttg
     3721 ttctgggaca acaaaatgcc atgacaatag cagtaaataa tgatacttcc gtaaggctcg
     3781 ccgtaaactg gggagaggtg aaattcctat gtgcttaccc aaagcggttc aaaaatttta
     3841 aactattatt ttaggagatg attttataaa atcgaaagcc tacttgaata aaaaacaagc
     3901 ctgtgaattt ctaggaattg ataataatac tttagataag tggattcgtg tatataattt
     3961 tccagtaata aaaattgatg gcgtagttcg ctttgacgta gaggatatca aagatttcat
     4021 gaatacattc aaaaaaaatt gaaattttct tctcgtcaca tatactataa atagcgaaat
     4081 gtactgcctt actttttaaa ggagtgagga agtttgccta caaagctttc aaatgggaaa
     4141 tataaaacta atctgaggta tccaaaaaaa ttcagagaaa tcactggaat tgcatcagag
     4201 aagtatcaaa aagtatttcc aactaggcaa ttagctatta aggccgaaag ggaaatgaaa
     4261 aagaagattg aaacagtttt aagagaggag aatgcaaact cactagaatt gaaaggaaaa
     4321 ataaaattta aagattttta tgaatcaaaa tggcttccta gatatgaact aggtcaaact
     4381 actaggtcaa acagggctcc aagtaatgta acaattagta atactaagga tatattccga
     4441 ttgcacatcc ttccaatgtt tggtgaatat gcaatgaatt atctgaattc aaatactgag
     4501 ataatatcag atgaattaac aaagaaatcg aaagaatacg caaatataaa aattatcaaa
     4561 ggctatgttc gctctatgtt tgatattgcc gaaatactat gttatatcga attcaaccga
     4621 actaccaaaa ttattcaatg tattaccgcg cctaagaaaa tggctttaga agaaaaaagg
     4681 ataagggaag ggaatcaagc attatcatca gaagagctga ctgattggat agaagcagta
     4741 aacaatgact taaataatca cttgcttaca cttcacgatt acacactttt catgcttacc
     4801 ttatatctag gtgatagaaa aagtgagact tatgctctgc aatggaaata tattgatttt
     4861 aataaaaata cggttcgtct caaatatgct ttagataaat atcaaagaaa gacacataca
     4921 aaaggttgga aagacaccgt tattcaggca ccagaagttg tgatgacatt attaagagaa
     4981 tggaaatcag ctcaggctga acaattgttg aaactaaaaa ttatacaaac acctgaccag
     5041 tacctcttta cctattccaa accttctgga gaagtaaatt gtcctgttca cgctgactac
     5101 ttaaactatc gaatcaatgc aataaaaaaa aggcatcctg aattggctca attatctcca
     5161 cacaaacttc gtcataccta tgcaactata gctagggaag gtggagcaaa tatgaatcaa
     5221 atctctaatg ctttaacgca ctcggatatt tctactacaa aaatatacgt gaacacacct
     5281 gacgtagttg ataaatctgt ttttgaagca tttcaaaaag gattgaataa gtgtgattaa
     5341 catgtgttct caaatgtgtt cttattataa aaaaagacac tttccaaata attgaaaagt
     5401 gtcctgaaat attgatataa aaagaatatt aacgttttga gaattgtgaa gctttacgag
     5461 cttttttaag acctggtttt ttacgttcga ccatacgtgc gtcacgagta agtaagccag
     5521 cacgtttaag agctaaacgg aagtcaggat caacttctag caatgcacga gcgataccat
     5581 gacggatagc tcctgattgt ccagcgtatc ctccgccgtt tacgttaaca aatacgtcat
     5641 atgcgccttt tgtttcagta acagcgaaag gttgattgat tacttcacgc aagtcagcat
     5701 gtggaatgta ttcttcaaca tcttttttat ttacagtaat tttgccagtt cctggtacta
     5761 gacgtacgcg agcaactgcg tttttacgac ggcctgtgcc gatatattga acttgtgcca
     5821 atgaaattcc ctcctattag attaagtttg tgatgtctaa aacttctggt tgttgtgctg
     5881 catgtggatg ttctgctcca ccgtatacgt tcaattttgt aaattgtttg cggcctaaag
     5941 tgtttttagg aagcatacct tttacagaag tttcgatcaa acgacgagaa tttttagcac
     6001 gcaattcgcc agcagtgatt gatttcaatc ctcctgggta gttgctgtga cggtaataaa
     6061 ttttgtcagt cgctttttta cctgctaatt tcactttgtc tgcattgatg acgatgacga
     6121 aatcgccagt atccaaatga ggtgtgaaag ttggtttatt tttaccacgt agtacagatg
     6181 caacaactgt tgaaagacgt cccaatggaa catcagttgc gtctactaca taccatttac
     6241 gttctacttc gcctggtttg gccatatatg ttgtacgcac gttattttcc tccaatttaa
     6301 gttctgatgt ttgacataca cttcgagttt ccggggctct ttgtggggca aacaatacca
     6361 tttaacatat tacccgctaa tctagtcaat gtcaacgttt ttctgcttga ttgaaaaatt
     6421 tatatgaaac tttacttctt ttctttcttt tatataaaag ggtcgtttcc tgtcaaaatg
     6481 aaaaacaaaa agaggctgtg acaaaagtct tgtcacaacc ccttattttg aataaacggt
     6541 gttctcctgc caactcctcg gtcacaagtc acaatatcca taaaacataa gaagctgttt
     6601 tttaaaattg tttcttgtga ctcggagctg aacggcaggc ttacaacctc ttggtaaacg
     6661 gtgttcaatc agctgaccct cggtattagc tcaaaaaacg gcaaaatatg aggaactatt
     6721 ttgacgtttg tttctctaat actcaggtca aaacgctgat ttcacaacct ctttttcggt
     6781 caagttcaac aacagttttt acgcgtcgat ctctggcgct ttgcccaaat gagcttggat
     6841 catccagata cgtttttctg tggctgtttt gaattcgata aagatatctt gtgttggatc
     6901 gtcgccttct tctccagaaa cttcgattcc tttttggtaa agatctgcta gataacgata
     6961 gcctgcaaca agtgtttcca aacgttctgg aatagtacga tcccagcggc caatttcatc
     7021 agggattttt gtgttatctg caaattcacg caatgtagaa taaggtgaac cgtctaatgt
     7081 gatcaagcgt tcggatactt catctaactg agcgttcaaa tcatccatga actcatccat
     7141 cattgggtgc aatgtaagga aacctggtcc tctcatgtac caatgtgttt ggtgaacaac
     7201 aacagacatt tggcttagat ctgcaactaa ttgatttaat acttctttgg tttgttggaa
     7261 tttcatcttt cttgcctcct aacattttct tacacttaaa ataatagtag actttcaatg
     7321 aaaagtacaa caaaatcatt tacctgagaa cgattttcag ttcgcttttt tatccgtaaa
     7381 gccaatcatt tttcagaatc agctaattct tctcgcagtt gagcaaattg ctgataagtc
     7441 tgtgctaaat gcttttgtgt cgaaacaaat aatggataga ttttctgata ttttttatga
     7501 tcagtttctt gtactccaac ttgctctttc aattctatca attcctgtgc ttcactgata
     7561 ctttggatct ttccgatgct tctccagcct aaaatcacag ctcctaatgc ggatgcttct
     7621 gtgacagtag ggaaaacaag cgggtaaccc agtacatcag ccatcatctg acgaaaaatc
     7681 ggagattgag caaacccgcc tgtagcacgg atctctgtga gctgattttc ctggcaaact
     7741 ccttttaaaa tccgataaag gttgagacag atcccttcta gaatcgcctt catcatttct
     7801 ttttctgtat gttgccgctg taaaccgata aaactgccaa acgcatctgc gtcccaaagc
     7861 ggtgctcgct caccaagtaa atagggatga aaaatcagac cattggcacc cgctggcaca
     7921 aaagcgatag ccgaaagcag tgcatcatat ggattttctc ctttttcttt agcagattga
     7981 acgacttcct gtaaaaatgt atgccctgcc caatcaaaga tgccagcacc attgctagtc
     8041 gctcctccca ctacccaatg tttctgatcc agaacataac aaaacgtctc catttctgga
     8101 tggaactgag gctgagtagt aataaaacga agtgctccgc tcgttcctac ggtcaaagcg
     8161 gcgatcgtat caccaaaagc acctaaccct agattagaaa gcggcccgtc tgcccctccc
     8221 aaaacaaatg atatatcatt tggcaaacct agttctgctg ctgtttttga tggtagacct
     8281 gttacttgat aggtggaagg aaagacttgc ggcaattgct ctattcgaat gttcaagtag
     8341 tcaagcactt cttctgccca ttggaattca tggatatcaa aatatccagt tcctgaagct
     8401 gttccgtagt cgctgatcag ttcgccaaac aatctataaa agatatagtc tttgataccg
     8461 acaaaataag cagttttttt gaataattca ggttggctgt tttgcagcca cttgattttg
     8521 ttcaatggtg tcattggatg aatcggcgtc ccagtctgcc gataaaattc tttgccaagc
     8581 acggtatatt tcagtttttc tgcttcgtcc gctgctcggt tatctgccca agtgatcatt
     8641 cgagataacg gtgttttctc ttcatccaac aatatcaggc catgcatcgc agaggagaag
     8701 gagacaaatt gaatgattgt gtctgaaggc aactgtttaa ttacttcttt tataacggtg
     8761 cagacacctt gaaagatcaa ttcctgatct tgttctgcca tcccttgtgt ttcttggatc
     8821 aaagggtagc gaaaataagc ggatgctgct tcttgggctt catctgtgaa agcaactgct
     8881 ttggtttgtg tggttccgat atctaatccg attgaaacgt tcattttttc acctcaaatt
     8941 ttatgtatgt acaaaaattt agtttaagct tgcgtatctt ggttttgaaa agggggagat
     9001 ggcgtgttac tttattttat cattggttgc tgtatcggat catttttatg tttaaccgcc
     9061 cagcggattc cgttaggtca ctctattatt tatcctagat ctcattgtgt caaatgctgt
     9121 cgttcgcttt cttggtatga attgattccg attctctcga tcatcgtcca acgatttcgc
     9181 tgccgctatt gcaggtgtcg gttgcccttc tactatttgt tggcagaagc cttatgtggt
     9241 ggtctttttg cctggttctt tactttttct tccgcaagaa atttttcgac gttcatatgg
     9301 atgctttcgg ctttgctatt ttcgttgatg gatttatttt acttcatggt ccattctcac
     9361 acgctattct gttcttgggc aattttatgg attttttggt tgcaggccgg cgtcttccaa
     9421 tggcaaagtg tgctgttaac gtttatcgta ggcggtgtgt gccttcgcta tggtcaatcc
     9481 tatctcggag ctggcgatac tctgttgatg ctttcttgga gtggtggctt gtctctagaa
     9541 gagttgcttc agatcttgtt tcttgctagt ctgtttggaa tatccgtttt ttctgtttac
     9601 tttatctgtc ataaaaaaaa gttggaaaag ctgcctttca ttcctttttt gagcagtgcg
     9661 ttgctgattg tccttcatct taggtattga aaaaaggttg tgacaaacga cttgtcacaa
     9721 cctctgatat atagtgttct cttaccaaat ccttgtgact tggagctagg cgacaggttc
     9781 acaacctctc cactaaatgg tgttcaatca gctgaccctc agtattagct caaaatgctg
     9841 gttttacaac ctctgatata cggtgttcca taagtagctt tttcggcttc ttatttcttg
     9901 acgccccaat gtcgacgaag gaaacctgca cgtccagaac cttctcgata aagattgtaa
     9961 tggagcttgt ttttcttgta ataatcctga tggtaatctt ctgcaggata aaattcgctt
    10021 cttggttcga tttttgtcac gattggctta tcgaatcgcc cgctgtcttg caatgcttgt
    10081 tttgaacgct ctgcaatatt tcgttcttct tcatcattat agtagatcac tggacgataa
    10141 gaatcaccgc gatcagcgaa ctgtcctaat gcgtcagtcg gatcggtttg ctgccaatag
    10201 atttccacca actgatcata agaaataact tctggatcat attcgatctc tactgcttct
    10261 gtatgccctg ttgtgcctgt tgttacttgt tcataagttg gatttggtac atgcccgcca
    10321 gtataaccgg atgtcacaga aatgatccct ggctgctctt caaatggttt gaccatacac
    10381 caaaagcatc cgcctgcgaa aactgctttt tttgtcatat aaaatccccc ttttttctca
    10441 tcataccttg atttccaaaa ggaggataac gttttgcttg tgacagaaaa aacggaaact
    10501 attatatacg tgatacggct tctgataaat gccaaaacga acgtacttga tcaaaaacgg
    10561 ttaaatcagg aatcacatat tcagcagaag gaagatttat gttttcacct attcccaata
    10621 catgcatgtt accagccaaa gctgcttgac aaccagcttc tgaatcttca atgaccaagc
    10681 atgcatttgg cggtacattc aattgctgtg ctcctttgag aaaaacttct ggatcaggtt
    10741 ttgcttttga tacttgcgtg ccgtcaataa ggacatcaaa ataacttgtc aaattcgttt
    10801 tttctaaaat gaggcgtgca tttttgcttg ctgatcccaa tccgatcttg atgtttgttt
    10861 ttttcaagta ctcaagaaca gctattgcac caggcaaaac ggatgtttca tccatttttt
    10921 gaatcgcttg aagatataga ttgttttttt gctcagcaaa ggctgatttc tctttttctg
    10981 agaatttctg ttccattttt ccaaatgaca gcaatcgctc tagtgactcg gttctggata
    11041 ttcctttcag ttgctcgttt tctttctcag tgaatggaat ggacagctgg ttagctaaaa
    11101 ccagccacgc ttcgtaatga taacgagctg tgtctacaag aacgccatct aaatcgaata
    11161 gtgctgcttt gattttcatc gattacctcc ttgaaaatga acgacttgaa gtaaagatgc
    11221 gatgttttta gctgtgcgat gaagattatc agtggcttgc tgtaaagctt cttgtaaagg
    11281 cacagctgca ggaaggatcg gaaaaacggc cgaaatgcca acatctgctg ctttttcgct
    11341 tcctttacca acacttccgc aaatagctat cacaggaacg ccctctggta ctcttctagc
    11401 aatcccaact ggcgcttttc cttttagact ttgtccatct agacgtcctt cgcctacgat
    11461 tacgaggtct gcttgctgta cccgtatcga actccagtaa atccaatacg tagtcgatgc
    11521 cagagactat tttccctgaa gcaaatgcta acagtcctgc acccattcca ccaccagcac
    11581 tactgcccga tgcctcgatt acttgagggg caaatctcat ataaaaatca tgcatggctt
    11641 ggtcagccac tgctaattca ttttctttca agcctttttg tggtccgaaa acaaaagaag
    11701 cgccttcttt tccgcataaa agattagtaa catcagtaat gacagtgatt ttggctttaa
    11761 tcagctcaat aggcacgttc gttttatcaa ctgtagcaat tttacctaga ttttctccca
    11821 gtgcttctac tttttgatta tcttgatcgt aaaaagcata acctagacct tctgccatgc
    11881 cgatgccgcc atcgttggta ctacttccgc caacgccgat gatgatgtgt cgaacacctt
    11941 gttgcaacaa ttgtcgtatc agttctccta ctcctcgcgt ggataaccta agtggctgtt
    12001 gttggtttag tgggacatct ttcaatccga cgatttctgc catttcaaaa atcgcagttt
    12061 gaccgtctga agcgtactgg atttctttgt ttttgccaaa tgcattagta acgactgctg
    12121 tttgccgctt caaccttaaa ctgtctgcta atgatgaaac cgttccttct cctccatcgc
    12181 ctataggtag cagatcatat tcagcctgaa caaaaacttc ttgaaaacct gattgaatgg
    12241 cttgtgctgc ctctaaggct gtcagacttt ctttgaaaga atctggcgca atcagaatac
    12301 gcattatttt cccctccgtt ccaagatcag ttctttttcc agctggtatt ggtctccatt
    12361 gacaaaaata ggtaatgttt ctttcgtctc tttttttatt actatttgtt tttttgtcaa
    12421 aacaaatagc aacctgtgat ttttccaaat aaaaggaaaa gaaatttttt cccatgctga
    12481 aggaagtctt ggagaaatag aaagagttcc attcgttact gtaatacctg aaaaaccaaa
    12541 tataactgct agccatagtg ctcctaatga agcagcatga attccgtcat ctgatgaatg
    12601 cggatctggt cctaaatcaa tcaggcacgc ttcttggaat aaatgatacg cccattccat
    12661 ttcttggata cggcaagcct ctatggcgtg aatcgcttta ctcaacgatg agtcgtggat
    12721 tgttttgttt tcatagtaat gcaaattctt agaaacgatt ggttgagaaa aatactcagg
    12781 taataagtat aacagcatga caagatctgc ttgcttgctg acttggagtt catttacttc
    12841 gctacgggca taatcaagta aaatagactg gctgccagga cgttctttgt aagtggtcaa
    12901 atcaataact ggcttactga gatacgtgtc atcttgcgga atcagctgtt ctttgttagg
    12961 cactggcaaa tataacttat ctaaaaaatt ttgacattct ttcaaaaaaa gcggatcgcc
    13021 agcttgatat ttaacagcta aggccacatt ataatgcgcc agatagttcg tatatgcatt
    13081 attgtcgata tattctgtgt attcatctgg accaatgact ctgtgtatct ctaaatgatc
    13141 atcgactttt acagctctgc tcagccaaaa agctgctgtt tcatgaatga gcgtttttcc
    13201 ctttgtttct aaaaacgatt gatcctctgt tgcttggaca tactcttgaa cggcataagc
    13261 aaggtctgca ctgatatgat gttctgcggc agctgaagca acttcttgtc ttgttccggt
    13321 tttgatattc aatgcagcaa atttgggtgt ctcttcctga ccagtgtgtg cgctttccca
    13381 tgggaaaagt gcaccttgat agtgataatc tcttgctttt tgccgtgcgc cttctaatcg
    13441 atcataacgg tacagcagca aattctttgc tacttcagga aactgatata aaaagaaagg
    13501 caggataaaa atttccgtat cccagaaaac atgcccttta taaccttcgc cagttagtcc
    13561 cttcgcccct acactaacac ttggatcatt gcggggagtc ataatctgta aatggtaagc
    13621 agcgaagtcc atcgctaact gatcgaaaga agaatcgcct tggatcgtta tccgatgctg
    13681 atgccaaaaa tctgtccaat aagacatcga ctgctctaaa atttctgtat aactaacgct
    13741 tttctgggct tgggcaatac ttttcttaaa cgggtctgtt tgctgaagag aggtagagag
    13801 cacgatgatc ttctccagcg taaaggacgt attttttctg atcttttctg aaaaggaagc
    13861 ggtgatccgg cgattttttg cttggaatac acctgcttta ttgaatttag ctgcaatccc
    13921 aatatcaatt ttactttctg ttgtctggta ggatgcactg atcaaatcgt caccaaatac
    13981 tcgcagttct ttttcaacca actgctgtgt accaaaattg gtttgctgtc catcgatacc
    14041 cgttttcaca tctacttggc agtcttcggt caacggcttg atgaccactt tcagggctgc
    14101 ctgatggaga ttattttgat cagcaacacg acaagtaatg acttccactt tttttccttg
    14161 agcagtttca atgagcgcat agcgtttaag ctctcccatt tctaaatcta gtgcccgctc
    14221 gtatgcaaga actttcatcg tctcaagaga aaaatgttca ccgtcgattt ttatttccca
    14281 tctgatgaca tctgggagat tcactaactc ggctgattct gctcctaaag gtcggttata
    14341 tacaccagct gcaaacattc cccttacttg attccgatag tcttcctcct ggcttcctct
    14401 gataccaaaa aatccatttc caatggtcat ctgactcgca tacttattga tatactctaa
    14461 atcaaactgt ttttcttcta ctttcataag gtaataactt ctcctgtttc tgccgaacga
    14521 tataaatttt ccaccagatt ctggatgaca tatccttgcc agccatctgc aatcatctga
    14581 gtctggtcac cgagcgcccg attcacaaaa tgagccatac ttagttcatg tgggtcatta
    14641 actggcgtgg tcttttgtaa ggaaaccagc tttccatcta catcttcgaa tagttcgcct
    14701 ggatacagat tagcacctgc ctgatcgcca caaaaagcca cattcagttt gtcttctctg
    14761 atattcaaag caaacgaagt attcagttgg atgatcttgc cgttttctaa ttccagtgtg
    14821 ccaaaaattg catcttctac tgtgaatttg cttggatccc atttcccgaa tttccctgaa
    14881 ttttttttcg taccaatttt ttggaagctg tacgcccgaa cgctttttac tttaggaaat
    14941 ccagtaatat acatcgctgt atcaagcatg tgaatgccat aatcgatcaa aggcccgcct
    15001 ccttgaagtt ctttgtcgat aaacgttccc cagcctggaa caccagaccg acgaagcgca
    15061 ttcgcttcta cgtaataaat atccccgaga acatcaagat gattcttgat cattgttgct
    15121 tcgtcagaaa aacgatgctg gaatccataa gccaaaatct gcccgttttt atcggctgtc
    15181 tctgccatct ttcttgcttg atcagctgtc atagctggtg gtttctcgca catcactgaa
    15241 gcattagctt cgagggcagc caatacatgt tcgaagtgaa agcgattggc tgtacagaca
    15301 ctcacgatat cgggagaaat ctcattcagc atttgtgtaa ctgatgtata cgcatgagaa
    15361 aatcctgtac gtcggcaaaa gtcttctgct cgctggctga ctggatcaac cacagcggcc
    15421 aattctaaga catctgatct tttttgataa taagcagcat gtactttttc tgctacttgt
    15481 ccagcgccta tgattgctac tttcttcatt tatttctctc tcctttgctt atttacttat
    15541 tgttattctt tctctattct ttctctaaat catttaaatg acgcgctgtt tccagacagt
    15601 ttttcaagta ttgcaatgat tcctgataaa ttttcacttc attttctcct cgcaaacgac
    15661 attcatatac catataacct tcatactcat tttgaagtag cgtttgaaaa tggcgagtaa
    15721 aatcaattgc gccagatcct ggttggtagc gatgattatc tgctaaatgg atatgaccca
    15781 catactcttt ttgcgtagtc aatgcttttg aaatatcatc ttcttcaata ttcatatgat
    15841 aaaaatcagc gatgatctgc acatgtttca attgattaga gtcaatgtaa tgacgcgcat
    15901 ccgctaacaa attgatcata tggtcttggt atcggttcaa aggctccaaa taaataaccg
    15961 tacctgtttc ttcggctact ttatctaaat aacgaagtga ttcgctgact gctttatagt
    16021 ctccttcttt gcttcggggt gaaaccatcg gcggtaatcg ataagtgaac attccccaag
    16081 ctgctgggat aacgattcct ttgcctccaa cctcgcttag tgcacgcaaa atctcagcaa
    16141 tttgacgtac accattaaga cgccgttctt cgataaagtc accgatccac ccatcgtagc
    16201 cgttacaagc tgtcactact gggatccctg tttctttgat tgcctgtttg actgtgtcga
    16261 tattttctat taacaactta ccgtcgattt cgtattcttc aaatccacag tctttgataa
    16321 atctaaattt ttctaaaatg tctcttggga aaaatgcttg atcttgcgta ccgaatttca
    16381 ttgttctcct cctcggactt gaacgcccat tttgatactt tcttctgggt gctggtctac
    16441 ataacgcatg taagcttctg cgctttctgc aaatggcaca actggatcaa tgatttcttc
    16501 acaatccaag taaccgttca tcaataattc ccaacatgtg tcctcgattc gtttacggtt
    16561 ccatcttggg taatctgggt ttggttcact agatgctcga gcaaagacga tttttgcatt
    16621 attgaaatgt gcttcgcgac caagattaaa cccagctgga aaaggtttag caaaggcaac
    16681 ataagaaatg atcccgccat agccgatccc tcttaaagaa gcttgtaatg cggcagcact
    16741 gccactcgtt tcaataactg aatctactcc acgtttatct gtcaattcct ttagacgaaa
    16801 accgacatct gctttcatag gatctagcgt gtaatcggca ccgtattttt ctgctaattc
    16861 tctacgctgc gctatgggat cgatcccaac gatggtagta gcacctgctt ttttagctaa
    16921 ttgaatcgca atttgcccga tagcgcctaa accgaagatt gctacagcat cacctgctcg
    16981 aacatgtgct tcccggatac cacctaaagc gaattgagca ggatcgtaac atactgcatt
    17041 tttccattga tccaaccgat caagtttacg tagtttataa ttatcaacac cgtttgtaat
    17101 cagcgtttca cgaatgggtc cataagtgca gactaactct cctttttgat actctgtcac
    17161 caaactgccc gtttcttcga tcgtaccgac aatcatattt cctaattgaa attcgccaaa
    17221 taccacaccg cgttggcttc cttcttctct tggcaagaat aggcgccatt cttctgaaaa
    17281 ttcttcatcg ataaatggac ttaatcctct aaagtcgaca atttctgttc ctggttttgg
    17341 agtagcgaaa attactcgga tacgtacttc gttcttttcg atctcacggt cttcgtaatc
    17401 tttgaatccg gctattcttg gttctaatgc aactaatttt ttcatctttt ctttcctctt
    17461 tcttttcttt ttatcctttt acgccgcctt cgactaaatc acctttaata aatcgttcag
    17521 ataacgcata cataatgatg actggtaaag cggtcaccaa tgaagcggcc atcattcgtc
    17581 cccaaatgta gtctggtgta ctgaataatg tgttcaaacc gattggtaaa gtaaattttt
    17641 cggaggtcga caggaaaatc gaagcaaata aataatcatt ccacgaaacc ataaagcaat
    17701 aaacgaagac agaaatgatt ccagaaatag ccaatggtat aatgatccga ataatgatct
    17761 ggatccggct aagaccatcg atcattgctg cttcttcaat atcagtcgga atcgtgtcaa
    17821 aataactttt tagcatgaat atcgcagtag gaagtgtttg aacgaccatc gtgataatca
    17881 gtgctgtatt ggtatcatac aaacctagac cggaaatgat tttaaataat ggaacaacga
    17941 tcaaaatacc ggaaaacata taaacggtgt aaaagctagc attgattttc gagcgagctt
    18001 tgaagcgtag ttttgacaaa gcgtatgccc cgctgatccc aatcactact gcagttacgg
    18061 aggctatcaa ggagatcact aaactatttt tgaaatacat gatgaatgga aaaatctcag
    18121 cattgaaaat atcctgataa tgttcaaaag tccattcttt cggaaaaata gtttgtgtcg
    18181 ttgcaattgc ttcttgtgaa tccttgaatg atgtcatcag catgatcaaa aatggaaaca
    18241 aattgacagc taatagcaac aaaatcgtcg cgtaaaataa gattttcctg ctctttttat
    18301 ttttcatttc aagtccacct tctttcgtga gagcaagacg accgctgcaa taatgatgaa
    18361 caatagaatc gatatagctg ctgcttttcc aagatcattg aacgcaaagg ctgtctgata
    18421 caaataaacg cctaagatat tgactttatt cgtcagtaga tagacatctg taaacatata
    18481 gaacatccag atagatcgta aaacaatcac agttgctagt actggtttga ttgccggcag
    18541 tgtcacgatc ttgaacttct gccaagcatt tgcaccgtcc atttcagctg cttcataaag
    18601 cgttttgtcg actgtttgca aaatagccaa aaatgaaata aacgcatacg gatagtatct
    18661 ccagatagca aacaaaacga ccataaagaa gctagcaact ggctggtcga accacagcgg
    18721 tgcttcgttg aaaagctgca acctatctac taacagatca ttgacgattc cataactatt
    18781 attgaacata tatttccaag caaaaatcaa agaaatagat ggcgtaacat aacttaataa
    18841 aatcagtgaa cgcaccgttt tcctaaatct gaacttgcgg ttaagcaaaa tagctacggc
    18901 taatcctaaa gctgtactgc ctgctacaac taagaatgtg taagcaactg ttaaacctaa
    18961 agaacgataa aattcactat cacttaaaat ttcaagataa ttgcttaatc cgataaaaac
    19021 ggcgtctgag ttaggattca gcggcatatc gaaaaaactg atctgaatat tggaaagcat
    19081 tggaatcagc actaaaacag ctaataaaag catgcttggt gctaaaagtc ccatggctaa
    19141 ttttaagtca gaatgtttcc ttggacgatt cattgaaacc tccccctgat aatctgccaa
    19201 tagctatcta cttttttcga acaccattgg cagaacttct tgttatttcg tatttttttg
    19261 tgcgtcagct aaagcttgat cgacatcttc gtttccgaca gttacttggt tcactgcttt
    19321 tccgatcaca cctgagcttg tgatatctcc cattttcgtg aaattctttt ccccgactag
    19381 tccaaagact tgtacttcat caaaagcact agcaatctca gatggcagct cgccaaaaga
    19441 cttgatgact tcattttctt tgtaaccatc cagttcgact acttgtttat tcactggttg
    19501 agcaccacca ggagacatca agacccactt ttccatgttt ttcggttgag ataagaaagc
    19561 tgtgaatgct tcagcagcct ctttttgttt ctcgtctaga ccagaagaaa tcgtcaatcc
    19621 agatactgtg ccatagacag cttgatcttt ttgtgttggg atagcaaaac cgatattttc
    19681 tgagttcccc gcttcataaa cagaaggcaa aatgtaagtt gaatagattg ccattggtgc
    19741 tgttccattc atgaaagcat ctttgatttc tgtcacatca ttagagcctg gcatcgtgta
    19801 ttgggataag ttttgataaa acgacaatgc ttctctcatc ttttccgtat cgatcgttac
    19861 ctcgccttca tcatccaaaa catttgctcc atttgacaaa gcaaattgag agaacgcttg
    19921 ctcactcatt gttccttcag cagtaggcat agcaattcca tattgtttat tctctttatc
    19981 tgtgaaatgt tgagcaattt ttaaaatatc atcccaattt tctggttctg aaaaaccagc
    20041 ttctgataat ttttctttgt cataccaaat accttgaacc cagccgctaa ttggtgcagc
    20101 aatatagctt tttccatctt ctgaacgaac aaggtttttg gctccgtcat aataattatc
    20161 ttctccagct tcctcgataa cgttttggac tgccgtctga tcaatcagtt catctttatc
    20221 catcacttta gcaaaatcct gactgacttc cattacttct ggtaatttac cggaacgagc
    20281 taacgtaacg atttttgtat tgaacgctga ttcttctacc ggcacttgct tgattttgat
    20341 gtctggattt tctttttcaa aatcagcaac cagttcattg atcacactta atcgttcctg
    20401 ttcgacagaa gagtgcatga attgaatcgt tgtctgttgt gattcgttag agctttgact
    20461 ttcaccacaa gcagtaagaa gaaatccagt agctagcaga caggtagtaa caataatttt
    20521 tttcattttt ttccacctct tattttatta ttttcagcca catatattca tacggctgga
    20581 atatcaactt tcgttcttga ctgcaatgga taatttgtcc agaaaacagg tctaggtaat
    20641 ctccttttgg taaaagataa cctgctgatt gattggaaaa attatggata actaagactt
    20701 tctcttcctt cgcttcgcga ataaaactga taaaagaatc tgctgagtcc agtaagtaaa
    20761 tcaaactatt cggatggaag gctttttctt tttttcgaat agatagcatc tgggtcaatt
    20821 caccatatac tgctgttctt aatggatctg tctgcaactc tttctcaata tctgctaaca
    20881 aaaatttttg ccgattgatt tttcgattct ctcctgtttt ttctacgcct ttgtaatcat
    20941 ttcgacttcc cagcacactt tgaatataaa ttgccggcac gcctggtaac gttaccaaaa
    21001 atgaatgagc agctaaaaag cgtttgcttc tccattccgt cgtatctgtt ggagaactta
    21061 acgcatctag atacgtagcg tttatctcat atggactggt cgttccatct gggtttttct
    21121 tgtagtttac tcgggcaccc tctttcttca gctgttccac taattcaagg atcgtttttt
    21181 ccggcagaat acctcgtacc ggattcaatc cgataccgtc atgggaggct aaaaaattaa
    21241 aaaaagttgt ttttttcgat gcgaaatcga tggtttttaa ccattctttc aacttacttg
    21301 tattacccga acgaatcgct tgaagggtca aaggtggtag cgaaaactga tagaccatct
    21361 gtgcttcatt ctcgccattc ccaaaataac tgatattatc tttgtgtggc acatttgtct
    21421 cagtgatcaa taccgttcct ggagctgctt catcagcgat tgcacggaat aattggatga
    21481 ttttatgcgt ttctggcagg tgtatacaag aagttcctag ttctttccat aaaaatccca
    21541 cagcgtccaa gcgaataaat gaagcgcctt tttctaagta aaacaaaaga atatcgatca
    21601 ttttcaataa aacttctgga tttttaaaat tcaagtctac ttgatcttcg ctaaaagtcg
    21661 tccatacgtg tttttctttc ccgtttttgt caatgaacgt tgtaagtaac gggctggtcc
    21721 ttggtctagt cacgctgctt gtatctgtat ccagtggcaa atcaataaaa aaatctttgt
    21781 acgtatcatt caagtttaaa tattcctgaa accaagtact ttcggcagac atatgattgc
    21841 agacaaagtc gaacattaac cgtgtttcgc ttctcatttc ttctatatct tcccatgtgc
    21901 ccaaacggtc gttgattgcc tgataattta caacggagaa accatcatca gatgtatatg
    21961 ggaaaaatgg caagaaatgt acaactctaa atgtatctgc caaatacttt tgataaaatt
    22021 tcttaaatgt acgcaaagtt ggttgctgct cttcaaaaaa ttgatcccca taagtaatca
    22081 agaaaatatc ttcttcgtcc caatgcagtt tgcctgcctg gcctttcttc tgctgataaa
    22141 cactgacttt ttgttcaatc atagaggcgg ttttctcaga caaatctgag tcgcgataaa
    22201 tcgcttgtaa aagttttgta tacatcggat tcccctttct tttttgggag cgttcccaac
    22261 gatttgatca
//
LOCUS       DAYYGO010000041        21993 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000041 DAYYGO010000000
VERSION     DAYYGO010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.041"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            202..1167
                     /locus_tag="ACHA5T_001289"
     CDS             202..1167
                     /locus_tag="ACHA5T_001289"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8886874.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(1266..2438)
                     /locus_tag="ACHA5T_001290"
     CDS             complement(1266..2438)
                     /locus_tag="ACHA5T_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8886875.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(2425..3570)
                     /locus_tag="ACHA5T_001291"
     CDS             complement(2425..3570)
                     /locus_tag="ACHA5T_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8886876.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(3584..4508)
                     /locus_tag="ACHA5T_001292"
                     /pseudo
     CDS             complement(3584..4508)
                     /locus_tag="ACHA5T_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            4625..5704
                     /locus_tag="ACHA5T_001293"
     CDS             4625..5704
                     /locus_tag="ACHA5T_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM8886877.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            5701..6329
                     /locus_tag="ACHA5T_001294"
                     /pseudo
     CDS             5701..6329
                     /locus_tag="ACHA5T_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(6439..6666)
                     /locus_tag="ACHA5T_001295"
     CDS             complement(6439..6666)
                     /locus_tag="ACHA5T_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8886878.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(6769..9357)
                     /locus_tag="ACHA5T_001296"
     CDS             complement(6769..9357)
                     /locus_tag="ACHA5T_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFM8886879.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(9377..10036)
                     /locus_tag="ACHA5T_001297"
     CDS             complement(9377..10036)
                     /locus_tag="ACHA5T_001297"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8886880.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(10085..10588)
                     /gene="trmL"
                     /locus_tag="ACHA5T_001298"
     CDS             complement(10085..10588)
                     /gene="trmL"
                     /locus_tag="ACHA5T_001298"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFM8886881.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(10809..11675)
                     /locus_tag="ACHA5T_001299"
     CDS             complement(10809..11675)
                     /locus_tag="ACHA5T_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFM8886882.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            11784..12425
                     /locus_tag="ACHA5T_001300"
     CDS             11784..12425
                     /locus_tag="ACHA5T_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFM8886883.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(12487..13848)
                     /gene="mgtE"
                     /locus_tag="ACHA5T_001301"
     CDS             complement(12487..13848)
                     /gene="mgtE"
                     /locus_tag="ACHA5T_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFM8886884.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(13866..14765)
                     /locus_tag="ACHA5T_001302"
     CDS             complement(13866..14765)
                     /locus_tag="ACHA5T_001302"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM8886885.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(14767..15564)
                     /locus_tag="ACHA5T_001303"
     CDS             complement(14767..15564)
                     /locus_tag="ACHA5T_001303"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFM8886886.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(15542..16246)
                     /locus_tag="ACHA5T_001304"
     CDS             complement(15542..16246)
                     /locus_tag="ACHA5T_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFM8886887.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            16377..16952
                     /locus_tag="ACHA5T_001305"
     CDS             16377..16952
                     /locus_tag="ACHA5T_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFM8886888.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            17070..17747
                     /locus_tag="ACHA5T_001306"
     CDS             17070..17747
                     /locus_tag="ACHA5T_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFM8886889.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(17826..19634)
                     /gene="pepF"
                     /locus_tag="ACHA5T_001307"
     CDS             complement(17826..19634)
                     /gene="pepF"
                     /locus_tag="ACHA5T_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFM8886890.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSDAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(19810..20808)
                     /locus_tag="ACHA5T_001308"
     CDS             complement(19810..20808)
                     /locus_tag="ACHA5T_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFM8886891.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(21034..21687)
                     /locus_tag="ACHA5T_001309"
     CDS             complement(21034..21687)
                     /locus_tag="ACHA5T_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFM8886892.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT         6492 a         4699 c         3814 g         6988 t
ORIGIN      
        1 cttttttgtg tttttcggtc ttcggacctt tttcattttc ctagacttct gctataataa
       61 ccaagaatta attttgtaac agattttttt gaaaaaacaa gtttctttca ttcaaaaaat
      121 agaaaaagct tgctaattat tggttaacca gcaaacagga gtattaaaat atcttgtttt
      181 attgaatagc aggaggaaat catgaaattc cattatcata tattgatcaa tccatcggca
      241 ggcagcggaa atggctataa agtagcagaa agaattttac cggtattaaa aaataagcat
      301 attgactata cacttcatta tagtgaatac aaaggacacg atgctgagat tgctgaaact
      361 ttggcaaaag aaacattgat tccttggata gaagaagaac agaaaacaat cgatactttc
      421 ccccttttga tcatcgtagg aggtgacggc accttacatc aggtacttga tactttctat
      481 cagatggaag tagaattccc agttgcttat attccagcag gttctggaga tgattttgct
      541 cgaggggcag gccttccaaa aaatcccaaa aaaggacttc agctgatttt agcagctcaa
      601 tcaccagaaa aagtccacat aatggcttat gaagaaaaaa taagtgaaaa aaaaggaata
      661 gctgttaata atttcggtat aggattagat gcagctatcg tccatgcgac aaatcattct
      721 tccacaaaaa agcgattgaa caaatacaac ttaggatcat tttcgtattt attctctatt
      781 ctccgtgctt tattcactca aaaaggattt ccaattctgg tagatgcagg aggaaaacaa
      841 ttaagttttt caaacgcatt tttatgtaca gcaacgaagc atcctttttt tggcggagga
      901 atcgcgattg ttccaactgc tgatgcgtct aaaccagtca ttgattttgt cgtggtcgaa
      961 cgaatcaatt tctttaaaat cttgtggctc attcttttac ttatacagaa aaaacaattg
     1021 aaatcgaaat atttccatca ctacacaaca aacagattac gtatcgtttc aacaacaccg
     1081 caacatggtc aagaagatgg agaagaaatg gaaaaacgac cttttgatat cacaataacc
     1141 acaactaaac agttgttttg gtgttaataa aagaggtttt gtcagaagct acatctggaa
     1201 caccgtttat tcggaaaaaa ggactgtgac acgacttttg tcacagtcct ttttttacta
     1261 tgcagttaat tctttgccca attgggttgc catcgatagt ttctttcttt acccaaaata
     1321 aattggatca ttgaaaacac cgcagcaaaa gcaactaaga cacaaccatt gaaccaaaaa
     1381 gattctgcaa atgtttgatt gtaactgagt gtttcactga tcaagttatt attttgaaca
     1441 aaccaatatg ttggtgtgaa tgccgctatt ttgctaacca tttctggcaa gtattctgct
     1501 ggtacaaaca ccccaccgat aaagcaactg cctgtgataa aaatatcttt gatcccaccg
     1561 ctggtttctg agttttttac caatgaagtg atacaagccg aaaagctaat gatcggcaat
     1621 aaaaacaaga aagtatttaa aatatgatac cataccgtta tatcccacga ggtgtggatc
     1681 actaacagaa ccattagaag taagaagcca ctgaacaaca gcagcgagta actaatcaaa
     1741 ctaaaggtta ctttgcgatt caagtttcgt ttggtaatgg gcgcactctg gtttcgttta
     1801 ctgatctctt tgcgattaaa tgctagattg atgacgctga aaccgctaaa aatggataga
     1861 aacaacccat aagacaagaa attaaaggtt gtgcccgtca acagcttttt catccgctga
     1921 ctatatcccg cacttagctc aacttctcca gtaaccgaga gtgccatttg tgtctgttcc
     1981 atagctgcat cgattgtttt atcttcttct tgaaacagaa tcagtgtttg caaaaaggtg
     2041 ttcacttgct gtgtaacaac tgttttagaa aaggcatctg ggcgactctg tatcttgatc
     2101 gtcggcatct ttcctacttc gacttgttcg gtaaagtctg caggcagatg taagataatg
     2161 tttacacgat taaaataaag agcatcatcg atttcttttt gtgaggttcc atccaatgta
     2221 acgactgttt ccttgtcatt caaatattta cgaaggccat cgactacagg cgactcttct
     2281 tcactaatca aggcgatttt cgcatcttgc agagaggtat tttcaacatc aatattttgc
     2341 gcaaaaaaga atgtggcaac aacagtgata attacaccta tcagtaaatt cgatttattt
     2401 gctttaagca atgtccaaat cgttttataa agcgtcataa cgcacccctc tttccatcca
     2461 agcatttcct aacgcaaaaa ggaaaataaa tccgatcaac catagaagat tggtataaaa
     2521 actagccaaa tcagtgtaga aataaagttt atacaaactt tcgctgatca agttgacgag
     2581 atttaattga ccaacaatcg gaatatgaag atcaatccaa tacttcactt gttccgaccc
     2641 catcatacca gcaaaaaagc tcatcgcaat cgtcactgaa atcaagatgc tatccttttg
     2701 caccagattc atttttggta tcaaattacc aaccaatgta cctaacaaaa tcgcacacaa
     2761 cgaccccaga gcggtaacaa tcatcaacca ttgccaacgt tcaccaaagt ctacttgata
     2821 gataaaacga aagacagcta acataatata aatctctgtc aaaacaatcg taaagcttgc
     2881 tgccaaatta gcgattgaaa ctaacgattg ctttctagga atcaaggaca agcgaatacc
     2941 atttgacgat tgatttgctt gctgatcctg tacattgcgg accccccaca tcgtcccgta
     3001 gagaatcgcc attccaatca gagtgaagaa ataaaaactt ttgaaactca tattacgacc
     3061 tgcatgaact tcttgaaaag atacttcttg ttcaaaaaca gtcatgatgg gcgcgagatc
     3121 aacgttctcc acttctgcca ctttactcga aattatacca atacgctgta aaaattgatc
     3181 catgacgttt ttcatcaacg tctgactcat tcctgcttga cccacatgga gcgaaacaga
     3241 tgaaccatca atgagataaa aaccagccaa atcatctgca tttagctttt cattcgcttc
     3301 tacttccgat agttcctgta cttcaaataa tggtttatca ttgacttcga cttgttctaa
     3361 gactgtggta aaattttccg aaagcgctgt ttgatccgct ttgattacaa ttccagtatt
     3421 aatcgtttcg agattgccac cttgatcaat ctcgccaaag gccgccataa acatcaatcc
     3481 taaaagaatt ggaaaggcca atgtccaaaa caacatggat ttatttcgca gcagtatttt
     3541 tgtgcgatat ttataaaaat gccaaaacat tatttcccct cctctagtct ctcaaatcct
     3601 tgccagtaat ctctaaaaag acatcattca aggacgcttc tttggttgct atttttccgt
     3661 aactgatctt ttcttcttgt aagattgtaa gaatcggcaa caatactgta tttagttcag
     3721 tagttgttac ttggagctga ccttttttat aagcgattcc atcagaagct attgttcgct
     3781 gacaacacgt tttctgggag taatgggatg tcgatcgtca aacgatccgc cgtccgtacc
     3841 atatctttta gagattcttt ggttccttta gcaatcacct gtccttgatc catgatcacg
     3901 atctggtcgc aaagaatttc tacttcttcc atgtaatgtg ttgtatagac aatggttgct
     3961 ccttgtcgat tcagctcctt gatactttct aaaattttat tccgactttg ggggtctaca
     4021 gctactgtcg gctcatctaa aaaaattaac cgcggcttgt gggcaatccc acatgcgatg
     4081 ttcaaccgac gtttcaagcc accactaagt tctcctggac gaaacgaaac atatttttct
     4141 aatcccacta attgaatcac ctcttcaatc aaggctttcc ttttctcttt ttcggaaaca
     4201 tataaaccgc aaaaataatc gatattttct tgcacactta gttcgtcaaa gaccgcaacg
     4261 tcttgaggaa cgaccccaat cattgccttg acagcataat tttcaggagt cattgttgtc
     4321 ccgaacaatt taatggttcc tttgtcataa tttaacaaag aaagcataca gttgatggca
     4381 gtcgatttcc cactgccgtt tggtcctaac agacctaaga cttctccttc ttgtacttgc
     4441 agatcaaaat gattcaatgc tacaaaatct ccatagcgtt tgactaattt atcaatttcg
     4501 ataatcatca tgaaaaactc cttccaaatt tcttgttgat ctgactataa taaagtgcaa
     4561 gaaaagaaac tagtgacgat tggctaaacc acacatgaca tttgtcacaa tgaaggagtc
     4621 tgctatgcat aaatctcggc tactcaacga aactagcttt ctactggtta tctcattgct
     4681 tttgctggta agtcaaggat tcgttgctat gcaagttatc tatttattga tcgccgtttt
     4741 attcactggt gtcagttatt tactgcctac aaagccaaat tggattttcc aagttattct
     4801 atttctttta tgtgcctttt tacctgatct tgtcctgttt ctgccagcga cgatgcgagt
     4861 gatcgcccct gaagaaagcc accttgatta cttgtttttg ttaggttttt tgttgatttc
     4921 gacggttggt aatcatcccc ccatcaaggt cgcctttgtc gtaattttgc tgtttttttc
     4981 cttttactta cgaaaaaaag atcaacgaac gaccgatctt gagaaaaagt acattgtctc
     5041 caaagatact cattgggaaa aacaacaaga gttatcggaa caaaataaag cattggccct
     5101 gtcacaagaa acacaattag ccttgcaaat tgtggaagaa cggaaccgaa ttgcccgaga
     5161 catccatgac aatgtcggtc atctgctgtc aagtgcgatt ttacaaattg gtgcgcttga
     5221 aatgatcaat caagaaccaa aactaaaaga accattagaa aatttaagtg ccaccgttca
     5281 cactgggatg gatcggattc gtcaaagtgt ccatgacctc catgagacat cgatctcttt
     5341 tcaacactcc ttagagtttc tgatcgctga ttttccttct tctgttacga ttgaaggaaa
     5401 cttgtttgaa ggattgaatg aaacgcagca aaactgcttc ttaatggtca tcaaagaagc
     5461 cttaaccaac agtatcaaac atagtcatgc caaaaccgtt gttttacatt ttcgaacgtt
     5521 gccggccttt tatcgcttgc aaatcacgaa tgatggcacc agcccacaaa aaaattctgc
     5581 tcccggtatt ggtttgaaaa cgatgcgtca acgtgttcaa gaacaaaagg gacaagtcca
     5641 tattttccaa gaaaaccagc aatttcagtt aacgatcatt ttaccaaggg agggacacca
     5701 atgataacag tagtcattat tgagatgatc catttgtgac gaaatcgtta caaaccattt
     5761 tagaaagcac gaaagagatc cgtgtgttag gcattggtca ttgtgccaag gatgcgctag
     5821 tcttatacga aacccatcgt cccgatgttt tgctgacgga catccgcatg cctaaacaaa
     5881 cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc caaagccatc cttttgcttt
     5941 tgacgacatt caaagatgaa gaatacatcc gcgaagcatt ttccatcggt gtcaaagggt
     6001 atttaatcaa acagaacttg caagcgatta ttccttctgt caaatccgct tataatggac
     6061 aggccgtatt tggtaatgaa atcatcgaga ctttcactca aatgatgaag aataaaccga
     6121 cgattgatag tcatgcttat gcttctttta gtgaacgtga gcttgcaatc atcaaagaag
     6181 tagctgccgg gaaaaacaat aaagaaattg cagatgcctt gtacttgagt gacggcactg
     6241 ttcgcaatta catcagccaa ttactagaaa agctagattt acgggatcgc acgcaattgg
     6301 ctatctattt ttatcagcat ttgcaataag tcttatctaa cacaaaaaaa cctgaatgat
     6361 gctccttcgc atcattcagg tttttttcac atcttctatt caattgtccc gcaaaccatt
     6421 tcctcacgat tgaaagaatc aaggattcgc agggctgcca ggattcgttg gattgttcgg
     6481 cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt tctagttttg attcttcacg
     6541 atcgtctcgt tctacatcag gactttcatt caatttgaat tctttttcca tctcatctaa
     6601 ctttttaaag tcattgccag attcttttgc taatttcgct aatttatctt cggtttcttt
     6661 agccataaag taacgccccc cttttgattt cagcttagca tatcacactg aaaaaacaaa
     6721 agaaaacgct aaccaacgat catttttagc tatttctcat ctttaaaatc aagcattttt
     6781 tgccggtata aaatcgtagg gagaactgtt ttccatacca atcatcggat ctactaactg
     6841 gttatcgatc acttctgctg taagaacgaa atctttagaa acagcaggtt ctacctcttc
     6901 tttcttttct tctccaacaa atgaagaaaa cagatctggt tccactgttt ccattacttg
     6961 gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt agcttttctt ttacaacccg
     7021 tggctctttt gtatagttcc catcttcaaa tggatcattt ccgaccaaag aatcttcata
     7081 agctgctagt ttggtccgga gagtgatatt cattttatca ttatccatga tccttgattt
     7141 caacgtagtc atccggcttg ctgattcatt cgctacacaa gtgacatagg cttgctcttc
     7201 ccctaacaaa atcaacagtt ccttactgcg agtcactgcc gtgtagagca aattacgctg
     7261 aagcatccgc tgatattgat ggaccatggg caaaataacc atcttgaatt cactgccttg
     7321 tgatttatgg attgagcaac aataagaaag agtgatttta ttccattcgt ttcttttata
     7381 agtgacctca tttgcatcaa actggataac taattcatcc actttatctt cagaatcttt
     7441 tgccaagatg atacccgtga tttctcccat atctccattg aatacattca attcgggagt
     7501 attgactaat tggaggactt tgtctcctat ccgataaacg gtgtcgttcc aatgtacttc
     7561 cttttttcca ggtttccctg gattgaatat ctcctgcatc attttattta acgcatcgat
     7621 ccctgcaggt ccccgataca ttggtgctaa tacttggata tcttgtgcac ggaatccctt
     7681 atttttcgct ttttccacaa ttttagagat caagggttca atttgataag catcacttcg
     7741 gaaataggaa cgatcttttt gattctttgt gaaatctttt ggcagacgtc cttctttgat
     7801 ctcatgggca agaggaataa tactagaacc atcaccttgc cgataaatct gattgagttc
     7861 cattttggga atttgttcga tttccaaaag atcatggagt acttgaccag gaccgacaga
     7921 gggaagctga tctttgtctc ctactaagat aacttgcata ttcgtcggaa tcgctttgaa
     7981 taatgtgtta gccagccatg tatcgaccat agacatctca tcaacaatca aaagtccgcc
     8041 ttctaattct ttcgtactga ctgaaggtgt tttttcccgt ccattcagcc ccagaagacg
     8101 atggatcgta ctggcaggca gacctgtcgt ttcgttcatt cgtttagctg ctcggccagt
     8161 tggtgctgca agtagaatag gaaaaatttc ttgggtatat tttgaaggat ccaattccag
     8221 tccgttcaac tctgcaaaaa gattgacgat tccgttgatc acagtagttt tccccgtacc
     8281 tggtcctcct gttaaaataa atagagggga acggattgct tcagcgatag cttgttcttg
     8341 cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc cgaatattct tctgcacttc
     8401 tttttcagga taatggattt cttttttacg actgagaagg cgctggatcg aattaccgat
     8461 cccccattca gaaaagtaca aactgttttc gtaaagatta gtctcttctt gttgtatctt
     8521 tccttcttca acaagttgga taattacagt agcaacctga tcaggctgga tttcaattgg
     8581 gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat gtctccgccg gtacgtatgt
     8641 atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag atagctgctc gaattcttct
     8701 cggtgaatct gctgcaatac cgatttgttc ggctagatta tctgcacgtt tgaacccgat
     8761 tccttctatg tcttcgacta gctggtacgg attttcttcg atgatatcca aagactcgtt
     8821 cttataggcc tggtagatac tgaaagctaa ctgactaccg aacccgtaac gattcagtcc
     8881 catgatcact tgatccatcc cgtggtttag ccgaatcgtc tcagcaagca tttctctttt
     8941 tggcttcgtc aaaccggaga tttgttttaa taccgaagga tcagcaatga ttttttcgat
     9001 agcatcttct cccagcaatt ctacaatttt ttctgctgtt ttcttcccga ttcctgggaa
     9061 tttttcgctg gaaagatacg ctacgatccc atttgcagtt gttggctttt cttgctcata
     9121 tctttcgagt ttaagctgcc ttccgtattt aggatgatcc actaattctc cataaaaacg
     9181 ataagtctca ttttcttgaa tatcgccgaa acttccggtg actacaatct ctttttcacg
     9241 gtaatcagct gtggtttcac tgatgtgtac caaaagcact ttgtaaaaat tactaggatt
     9301 ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc atttcttctg gctgcatcgg
     9361 aagccgcctc cttcttctat agtaaaggtt ctggtgctgt ttcagttcct aaaaaatctg
     9421 tttggttcca tactttcagt tcatatggca ataatctgca aggctcttca gtctctaaaa
     9481 tcgtcaaact actattgaac aatccaccca tttcacgcaa ctcacttagc tcttttcctg
     9541 tcatccattg aatggctgca gttaaagaag ctccatgtcc tacgaataaa agtgggccat
     9601 ctttatgttg ggcaaccgca tctgctacaa cagtttcgat acgtgtgatt gccgattcaa
     9661 tcgtttctcc gtgaaaagga gaaggatcat acagatctaa atgattacgc aaattcggca
     9721 gattatccgg atatttctgg tacatttcat cgatcgactg accttctaaa gtacccagcc
     9781 ctagttctct tagcttatct gtataaaaaa tctctaccgg acgagcaagc tctttgttga
     9841 taccttctgc tgtatcacgt gctcgctttg ctgtactgga ataaattgct ttaaaaggaa
     9901 catattttac ctttttccca aatgctttga tttcttcgta actttgtggc aaaagcggag
     9961 aatcgccatt gctcccttga aaacgtcttt cctgattcca ttgtgtcttc ccatgtcggg
    10021 taaaatatag ttccatacgc agcaaactcc tgtctttctt ttattttact ctataataaa
    10081 ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa ttctaaatta gggaatcctt
    10141 gctggcgaag tgcttcatac actatcattg cagctgtatt cgataaattc aatgagcgga
    10201 catgctcatc attcataggg atgcgtaaac acttttcttc gttttcacgc ataaatgttt
    10261 ctggcaaacc ggttgtttct tttccaaata aaaagaagtg ctcttctcca tcagcaaaat
    10321 tttcgtcact atatacttta tgggcaaatt ttgagatcaa atgtagttta cgattaccta
    10381 aaaatgttaa aaaagcttct aaattactgt ggtaagtaat atttacatca ttccaataat
    10441 ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa acctagtggc tcgattaaat
    10501 gaagcggagt attcgtggct gcacacgtac gggcaatatt tccagtgttt gcagggatta
    10561 atggttcaaa taaaacgata tgattcatca ggatcattcc tctcatattt atgttcgttt
    10621 ctattttctg acaatttgat ttttgcataa aaaaaaacgt atcaactcgg tgtcagtcac
    10681 tgcgagtcgt tacgttagtg aagctcttct cactaacttt tatcagaaca ggaacctgat
    10741 aaaaaccaat gaaaaaacaa cttgttgcta atataacacc ctcaactttt ttttgcaacc
    10801 tctactcatt aaaaactgct agaaaaacgt tttctcttag ttccttttaa taattctatg
    10861 tcaattgtaa tcttcttcaa aggattctta tataagtctg ctttgattcg ctcttctacc
    10921 tgccattcta aaggtgacat ggccaaaaga tctttttgat tttcctctgg tatctcaaat
    10981 tcataggtta cacgctgacg ttcagttaat ttcattgctt cagcaaactt ggctactaca
    11041 cgttcattgg aataactctg tttttcaggt tgattcggat aaaaagcctg acgtagttcc
    11101 tttaaataat tttcttgtgg caccaccttc aacacttctc catcttctga gagtacacgt
    11161 tggaattctt ggtaatgaga aggcgaaaag atatttaaaa ccgcgttgaa tgtttgatca
    11221 gcaaacggca aattggtcaa atcagctaca caccaaaatg cttcggcaga ttgttctgta
    11281 gctgcgtaga ctccttcttt tgaaatatca aatccaacag cattttgaat ctttttcaat
    11341 tttgaaagtt cattcaagaa gctaccttct ccgcagccaa catctaatag cgagacttct
    11401 tcaggtaaat gctgcgcaat agtctcaata acgggctgat acatgccaga ttggatcatt
    11461 ctacgccttg gaacgaacat tccttgatcg tattctgttt taaccgcatg ggataaaaaa
    11521 taaagtgttc cttttttgga aaggtcaaaa cggtgattat ttttgcatat caagccatta
    11581 tcagactttt ttacttcttc ttggcaaacc ggacagcgga acagctgatg attttctgcc
    11641 aaaaactggc gtcctcggtc aatttttttt aacatagttc cccctcattt ctgttttatc
    11701 ttaccgttag atgctcactt tggcaactgc taaaaatatg atacaatgcc tagaagaaag
    11761 tgaaagataa aggagaagat tagatgatca aagaattttg cgctgaaaac tacacatcta
    11821 ttcctctagc aattgctaga ggagccgatc ggatcgaatt gtgtgacaac ttagcggtcg
    11881 gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt aagttatgct ggggaaaaag
    11941 atatccccgt aatgacgatt attcgtccta gagggggcga cttcgtctat aacgatatcg
    12001 aattgaaaat catgcatact gatttgattg aagcaaagaa attagggacg gatggcgtag
    12061 tatttggttg tcttactcct tcaggctggc tagacgaaga ggccttagaa ctattgatcg
    12121 aaacagcaga agggttgcag atcaccttcc atatggcttt tgacagtatt cctgaagatc
    12181 ggcaattcga agcaatcgat tggctggtag aacatggtgt tgaccgtatc ttgactcatg
    12241 gcggtcctgc tgacaaaact attgtagaaa atttcccacg gcttaaagaa ttgattgcct
    12301 atacagatgg ccgcttaatc attttgcctg gtggtggtgt cactgcagag aatgcagcac
    12361 atgtagctaa agaattgaac gtaagcgaag tacacggaac caaaatcgtg gatcttcagt
    12421 tataatctta taaacaaaaa aacatttgca acaggcataa tgcttattgc aaatgttttt
    12481 gtttttttac ataaaatagc ccataaataa agaagcaata ttgaaataaa tcagtacact
    12541 cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct actgctggat caaatcctag
    12601 acgatccatt agcatcggga ttaaactacc tgcaagattc gccactgtga ttgcaaatag
    12661 catagccatc cctatgacaa accccaagat aaaattatgt ttccaaaaac ctacgattag
    12721 aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg gtcaagactt cagccaaaac
    12781 aagtcgaaca aatccatgat ctttctcatc agaaatcgca agtcgacgaa cagccacagc
    12841 tagcgactgg gttccagcat taccggcagt tcctgtaatc agcgaaatga agactgctaa
    12901 aatactcgct tcacttacca agtcttcata gtgactgatt aaagaagcgg tcgacatacc
    12961 taaaaacaac agagtgatca gccaaggcaa acgtcgagac gctgccttta ctggattttc
    13021 attaatttct tccacattga ccccggcaag tcctgaataa tcgctagcag cttcatcatc
    13081 aataacgtcg atgatatcat cgactgtgac gattcctaac aaatgatcat cataatcggt
    13141 aacaggaaga gctaagaagt cataatctct aaatgtttgt gccacatctt cttggtcgtc
    13201 gcctacatga actgaaagca ctcgttcgct catcagatca gaaatcattg cgtcgtcatc
    13261 attcacgatt aggtcgcgca gagaaataac ccctactagc tgaccttctt gattaacgac
    13321 atagatgtaa tagattgttt cagctacatc tgcttcgttc ttcagcacgt acatcgcaga
    13381 gcgaacagtt tgattggcta cgatcgaaac aaattcagtt gtcatgatcg cgccagccgt
    13441 ttcatcctcg taatggagaa gttccttgat ttcactggca tcgtctgccg acatcaaact
    13501 taaatattta gcaatttgct ttttatccag cgtattaagc aagtcaacag cattatctgt
    13561 atacatctca gaaagcatat cagctgcata gctaggacgc atttcatcta ggtattcttt
    13621 catatgttcg ttgtcttctt caatcacatc gaacatatcc gctaattctt taggcgacag
    13681 ataggaatag atcagctgcc tgttctcttc tgtaagagat tggtagaatt gcccctgctc
    13741 atagatatgc atctctaaaa aactgtttct gaatgcctga atattatttt cccttaaatc
    13801 ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt tcatccaaac caatctcacc
    13861 tcgccttatt cttcaaaccg tctcacaact gcctccatgt ctgcggctaa aggctgcttg
    13921 aactccagca actcttctgt aaatggatgg taaaaacgca gatcataaca atgaagggct
    13981 tgtcttttta ttcccagatc cattcgcccg ccatacatat catcaccgag cagggcacag
    14041 ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc ctgtatgcag ctggatatca
    14101 actaacgcga tttctgttag tcgcttctct atccagtact cagtctgtgc cgaacgtcct
    14161 tcatcagaga ccattcgctt caatagcgaa gaataatctc gggcgatcgg caaatcgata
    14221 tgtccatgtg ccgataaatc tgaaagcctt ccactgacta aggcctgata ttttttcaca
    14281 aacttctttt cacgaagttg catatctaat tttgcgtgcg caaatccatg ctttgcaaaa
    14341 agcatcaaac cagatgtatc ccgatctagt cgcgtcacca catggattac ttggttttcg
    14401 taaccttgtt ttttataata agcctttaca cgattagcca ttgtcccatt tggatggtat
    14461 tgggcaggaa tagacgaaac gcctgctggt ttatttacta ccagaacgtg ttcatcttca
    14521 tacacgatat cgatcggtgt ttcatccaaa agtacggttt catgttctcc ttcagcagga
    14581 atcacaatgg ttaccttatc atccttagct agagaaaaca agacattttc tacttgatca
    14641 ttaactttga tttgaccgcc ttgaaatttt attttcgcta acagaccctt tgagatccct
    14701 ttttctttta aaaaatattt tacttgctga gaatgatctt tctcataatc ccatgtaaac
    14761 tgcatatcaa tcctcactaa taaaggcatc tttcacacga tgccaaaaat gcatatgtcg
    14821 ataagaagca aaatggatcc gttcttctgc tattcgataa taaattgagc ggatatttgc
    14881 ttttgcgaca ttcaattgat caaccgttac aagataatct tctgtatttt ccaattttat
    14941 ttctagccat tcatcatggg caatcacgat cggagaaccc aaagtacgaa acactcgatt
    15001 attcagtgaa gcaatctctg ccaactgaaa ggcattgata ctaggatgga tcacagcacc
    15061 gccgacactt ttattatatg ctgtcgaacc agtaggcgta gaaatcgtca atccatctcc
    15121 tctaaaactt tcaaacagct cgtttttgat gtatacatcg gctaccatcg tacgattggc
    15181 ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg tccggtgttt catccaaata
    15241 actgatccga acatccagca agggataact aacactttgt tcccggttcg ttcgtaagct
    15301 gtctactagt tcttccagct cgtaatcccg ccaatccgtg taaaacccta aatgacctgt
    15361 atgcacgccc aagaaacgca catcattcag acaatgactg tagtgatgga acgccgacaa
    15421 taatgtgccg tctccgccga ctgagatgac caattctggc tcatgatcat ctcgttgaat
    15481 ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact tctatagtct ttgcttcttt
    15541 attatgaacg atcgctactt tcatcccctg cctcctctct ttcgctttct ttcttcaaaa
    15601 gcgttcgata gtagacatca tctgattttc ctcttccatg ggagaataac cgctgtgctt
    15661 cttggatttc ttcacggata ctggacatct cttcatccaa ctgataggca gcttctgccg
    15721 cacgctgaag tcgttcactc atttcttctg gaaaaacgcc ttgatattta taattcagcg
    15781 aatgctcaat cgttgcccag aaattcatcg caagagtccg aatctggatt tccgccaaaa
    15841 ttttcttctc accgctgatc agctgtacag gatactcgat caccagatga taagaacggt
    15901 agccacttgc ttttttattt gtgatgtaat ctcgttcttg gagaactttc atgtcttttc
    15961 ttttacggat gatctcaaca acttgatgga tatcttctac aaactgacac atgatcctta
    16021 acccggcaat atcagacatt tcttcttcta aacgtcccat tggaatatgc cgtaattgtg
    16081 attttgttac aatactatcg actggcttga ctctcccagt gacaaattca atcgggacat
    16141 gtcgattttg ttcacggaac tgtttgcgga tcccccgcag tttcactttc aattctgata
    16201 cagtttgttc atatggtgct aaaaatgctt cccaatctcg ttccatagct gatacctcta
    16261 cttcattaac attcattgtt tattctacca tatatgcaaa gttttttcgt actcttgatt
    16321 tatgcattga ccaaaaaaag aaaattaggc aaaatagaca aaaaggagtc gatcaaatga
    16381 cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa gcaagagtac gagatattca
    16441 tcgctcacta tcaattaaca gataaagatt tccgtgtaca gactaatatt tatttcgata
    16501 ccgttgatga acagctgaag aaacaaaatt gtggcctacg gatccgtttc gttggtcatc
    16561 aagctgaata tacattaaaa acacctgctg aaaaagggcg tctcgaaaca acggatacct
    16621 ttataacaga agaagcagaa gcatttatct tcgaaaaacg tctgcctcgt tcaggcgcag
    16681 tttttcaaaa actttctgat ttgaatatcg atcctgcttc attaatccaa accggaaagc
    16741 ttacaacaaa acgggctgaa ttccctattg aagaaggact tttagctatt gatgaaagct
    16801 ggggagacaa tctacacgat tttgaactgg aactagaggt aggggatgca tctgtgggaa
    16861 aaattgcttt tataaatttt ttaaaacgtt tttctttacc ttatcgtcct gctaagaaca
    16921 agatccaacg tatgcttgaa gctaaaaatt agaaaccttt cattttcaca aggcacttga
    16981 gttcattttt ttactttttt ctgttaagtt ataggatgtg aaagaatcta gtcactagtt
    17041 gtatagaatc tattttgggg gagattcata tgatcgaaat ctatttattt gttaatcctt
    17101 taggggaata ctgtttcgaa atggaacaac agttattaca atttatcgaa gaagagtacg
    17161 ggacaccatc aaaagaaaag atgcaatttc gctttcttcc tttggtcaat ttgcagacaa
    17221 ttggggatgt catgcaacaa aaaggaattt ctcagaacaa tcttgaggaa cgtaatcact
    17281 tattctcaat gacttattct gcagcattgg actgcaaagc ggcgcagttt caaggaaaga
    17341 aaaaaggccg ccgttttttg atcaaattgc aagaagcagt cggctgtaat gacataccat
    17401 attctcaaga acttgctgaa tccatatttg aagaaatcgg cggcgatttg aatatgttta
    17461 aagaagatcg tcagtctgac ttcgttaaag aagtattctt ttctgatcaa aaaattgctc
    17521 gggaaatggg catcacaaaa cacccttctg cagtcgttta caattatgcc tgcgaccgtg
    17581 attttggtgt tttagtagaa gtaaaaggta caagtagcct ttggagaatc acagagctgt
    17641 gtaaaacaac agataactca tatcaagttt ttaatggtga acatttacat acaaatgatt
    17701 ctcagttcca ttcaagaact agccatttac acttgctttc taattaaaaa aatacgtcga
    17761 ctcccttaca agagagccga cgtatttttt tcgtttctat tcgagaaaat caaaaataga
    17821 aagccttata aacgatcgat caattcttct aattcattta gacgttgttc aaacatcgac
    17881 attgcatctt caatatatgc agcctgtgtc atatcaactc ctgctttttt cattacttcc
    17941 acaggataat cgctgttacc tgcttttagg taagccaagt agttctctag tgcctctggt
    18001 tcctggttta gtattttctt cgccagtgct gaagcagcag aaaagccagt cgagtattgg
    18061 aaaacatagt aattataata aaaatgcggt atccgtgacc attcgaattt aatttctggg
    18121 tcttcttcga ctgctggacc ataatatttt gcattcaatt taccataact atcacttagg
    18181 tattcactag tcaatggcac acctttttca tcttccgtat gcatgaaatg ctcgaattca
    18241 gcgaattgtg tctggcggaa aactgttcct ttaaacccat ccaagtaatg gttgagtaca
    18301 taagcccgta cacgagggtc tttttctgtt tccaataaat actccgttag gatattttca
    18361 ttcgttgtcg aagcaatttc tgctaaaaag atggagtagt cgccgtacac ataaggctgg
    18421 ttcgaacgag tgaaataact atgaacacta tgtcccattt cgtggacaag cgtaaatagc
    18481 tgatccagcg tatcatgcca attcaataaa atatatggat ttgtgtcata gcttcccgaa
    18541 gaataagcat cgcttcgttt ccctttattt tcgacaacat cgatccaacg ttcagagaat
    18601 gctttttcta cgatggccat gtattcttca cccattggtt tcagtgcttc taaagctttt
    18661 tcttttgctt cttcgtacgt aaaggtaatt ggagcttccc ctaagaccgg tgtataaaga
    18721 tcatacatgt gcagtttttc cacttctaat aaacgtttcc gtaattccat gtatcgatgt
    18781 aacaaaggca aatgcttgtt taccacgtct accaaagtat cgtatacact ttcaggaata
    18841 tgattattgc tcaaagacgc ttctctggca gagctgtaat tacggacttt cgctttaaaa
    18901 ttatgtcctt ttatatgtgt gcctaaagta gaagcaaatg tatttctaaa ttgttcgtaa
    18961 acactgtaca atcccttaaa tgctgcttcg cgcacccttc gatccgtgct ttctagcaac
    19021 tgaccataca cgccatgaga taattggact atttcaccat tttctccttc aatcgttgga
    19081 aaaactagat ctgcattatt caaaacagca aatgtatcac ttgaagcatc aaagatttca
    19141 cctgctccag caagaagaga ttcctgctca gcagataaga catgggctcg attatctact
    19201 atttgctgga tataatggcg atagacttcc aattttggtt cttctttgaa atattgccaa
    19261 atctggtcat ctgacaattg caatatttct ggttcaaacc aggaaacggc ttcactaact
    19321 ttcgaaaaca gactgcttgc tcttgcataa agcgcttggt aatctgtatt tccagtgtct
    19381 tgatcgtttt taaggtgcgc atatacataa atgacttcag tttggcgata aaccctcaat
    19441 acgaattcaa tcgcatttaa aaattgagaa gcgccttgat caagtgttcc tttgtgtttt
    19501 tcagattgtt ttaactcttc tgataattcc aaatatttct catcaaactc ttggtcgctt
    19561 gaaaagatct tggtcaagtc ccaagttaaa ttttcaggca attcttctcg ttttggcaac
    19621 tgctttactt ccatgaaatc cactcctttt cttcccttag ctctatctat caaaatagat
    19681 catagtctaa catccacatg tcacagcatc taacttttct actcattcat gtgtctgctt
    19741 ccatcaattt tagcataact aaatcttgtt ttacatagaa aaccgattcc tagcctattt
    19801 ttatacttac tattttttca aagaagacaa gtgtatgcat tcgttaaaaa actgctgata
    19861 gaccatctct tcatcaatca taggaaaaag ccattccctt ttatgttctt ttatttggca
    19921 aagccatgag ttgaaatcag gaactttctg gttttccaag gcttcttcgt acaacattcg
    19981 ataaagaaaa acctgttctt gtaaataaaa gaaaaaccga cttttttgat agatccacga
    20041 tgatagatgc aaaagatgtt tatgacgcaa gtagcattgt tcttgtatgg atactgtatt
    20101 cttttgcttg tgaataagtt ggcgtgtcag ccatttcttc ccatcttcac tcactttttc
    20161 tatttttttc atttggttca ttttaatttc gttactagtg aatatttctt taaggtccct
    20221 actagaaaaa gtccagcagc tactgcaaaa ggaaatatgc cctgatacag tctctgtaat
    20281 atgatgatac aaataaagct tttgtttctt gagatcagct ttccagcaat gcactcctct
    20341 ttttctatta tagtagcagc aacttttttc tatcaatgag aattgcgatt gtccaagaaa
    20401 agaattacca agtatccacc atacttggta actgttattc aaataatttt gggttctttc
    20461 aataaatctc tggtgagaca gtctagaaca ttggatttct attgctagat tcgccaacaa
    20521 aatatccggc ctttgccgca acctcggcaa ataggcttcc aaaaatactg gttctactga
    20581 ctgtcccagc caatccatga agcattcctt taactgtaag tgctcttttg attctctttc
    20641 actaaagacc gaacagttat ctgatttttg atgggcaaaa tgagcacatt ttttctttcc
    20701 ctttttcaaa atcactcttt ttcgacagcc acgacaataa tatttctttc ctttttctgc
    20761 atgtttagca aaacagcact tattatattc attcattgca agcagcattt ttctccctct
    20821 gaacttattt acgtctaaga atttatttac acgaaaaaag agggcgtgaa aaagctattc
    20881 tgcatcaagt aataagaaca gctaaacaaa aatagttccc aatatttttc gtttggttgg
    20941 gcttgttacc gcagatgcaa gcttttgaac acatttatac gaaaaaaaga ctgcgacaca
    21001 atttttgtca cagtctctta ctataatgct gatttataca aaataaaatt tcgttcgctc
    21061 gatagcatta cgcgccatca gacattgtcc atattcattc aatgtttctg gtgtacgatc
    21121 gctcttttca gcaaactcca aaattcgagc aatctgattc tctacatccg cttggttgag
    21181 attttccatc cagaagtgaa cgactaggta atagcgctct tcgaataaat acaaatcagt
    21241 ccaagcagac tctagttcga catctgttgc aagttggatc acttgttcaa aatcttcaaa
    21301 agcaaaagtt gcttgtcttt cctgttcttc tagggcgctt ccagataatt cttcctccgg
    21361 atcgctgcca ataatctgct gacgaagaaa gtcactcaca tcttctgaat caacagtaga
    21421 atcttctgat tgagaaattt cttcatccgg aagattttta ctgataaaca gttctaatcc
    21481 gtctcctttt ggcaaaactt ggaaagtgac agcttcgctt cctcggaact cctcttcgat
    21541 atcaacttct tccaaaatac tataaaagaa attttcgatt tctttgtgat tgccaagtaa
    21601 atccaaaaat gaaacgcccc gttcctctaa atcttcatga ccaattagta cacggatcgt
    21661 attttcatta atatgttcca tttccatttt gctacacctc actttgccac aatgtttaat
    21721 atagattcat tgtagcgaaa tcagcttaaa tgtaaagaaa aaaaatctcg ctgttaagcg
    21781 aatttaataa aaccagcgga tttttcaacc tttcaagcta tcttatttaa ctataacatt
    21841 tttggcagac gaatactata tagccgactt ttcagctaat tttccctctc tatttttcgt
    21901 gcaatcatca tcttctacta cctttttata catctaatca agaatacttt ttgtaaaaac
    21961 aaaaaaactt ggaaaaacta tctttgtaat agt
//
LOCUS       DAYYGO010000042        21868 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000042 DAYYGO010000000
VERSION     DAYYGO010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21868
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.042"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(19..564)
                     /locus_tag="ACHA5T_001310"
     CDS             complement(19..564)
                     /locus_tag="ACHA5T_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFM8886893.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(576..1268)
                     /locus_tag="ACHA5T_001311"
     CDS             complement(576..1268)
                     /locus_tag="ACHA5T_001311"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFM8886894.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1287..1571)
                     /gene="macP"
                     /locus_tag="ACHA5T_001312"
     CDS             complement(1287..1571)
                     /gene="macP"
                     /locus_tag="ACHA5T_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFM8886895.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1584..2147)
                     /locus_tag="ACHA5T_001313"
     CDS             complement(1584..2147)
                     /locus_tag="ACHA5T_001313"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFM8886896.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2375..3037
                     /locus_tag="ACHA5T_001314"
     CDS             2375..3037
                     /locus_tag="ACHA5T_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFM8886897.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3161..4357
                     /locus_tag="ACHA5T_001315"
     CDS             3161..4357
                     /locus_tag="ACHA5T_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFM8886898.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4458..5834)
                     /locus_tag="ACHA5T_001316"
     CDS             complement(4458..5834)
                     /locus_tag="ACHA5T_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8886899.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5871..6737)
                     /locus_tag="ACHA5T_001317"
     CDS             complement(5871..6737)
                     /locus_tag="ACHA5T_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFM8886900.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6826..7851)
                     /locus_tag="ACHA5T_001318"
     CDS             complement(6826..7851)
                     /locus_tag="ACHA5T_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8886901.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7869..8543)
                     /locus_tag="ACHA5T_001319"
     CDS             complement(7869..8543)
                     /locus_tag="ACHA5T_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8886902.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8727..9620)
                     /locus_tag="ACHA5T_001320"
     CDS             complement(8727..9620)
                     /locus_tag="ACHA5T_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8886903.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9694..11391)
                     /locus_tag="ACHA5T_001321"
     CDS             complement(9694..11391)
                     /locus_tag="ACHA5T_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM8886904.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11384..12697)
                     /locus_tag="ACHA5T_001322"
     CDS             complement(11384..12697)
                     /locus_tag="ACHA5T_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFM8886905.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12697..16638)
                     /locus_tag="ACHA5T_001323"
     CDS             complement(12697..16638)
                     /locus_tag="ACHA5T_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM8886906.1"
                     /translation="MEKLDEPIVPSGQALQRIQRQLFNGILERLGPTATQDPYEYIYP
                     SGEVENEQHRYPKFTTNDYTKVLGNWTQSNERYQGDKGSPYDGENLIDDSDYTNGGAN
                     WRNYDYATNNGSTAGEEDRNVPAQTVQLWGNERNFENSYLDYNGAYIKKWVEPVLPSS
                     TASDLHPEDATTLYNVYLDVIGGEKKEISPIDIVFVLDKSASMSELTAGTNSQTKNAA
                     LIEAVNEMSKDLLSDPSLDIRIGMVNFYHNSTAINNHEQISSDIFPLTNDINRLTGSE
                     NTALNRTPIGGTPLTLGLKNGYETLYKDNGGENRNPEKILIVVGDGTPTFSYAPIQSS
                     YRTSTNGAWSNWTVMEDKIAEDNDVLFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDE
                     VNVHYRYGEVKEGDNKATHWVGDGSSNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQE
                     NPPSIFSIGLGIDGNVSGRQRLDAIGRNVLKNIADLEEDGVTPRYYNANNKNDIVTAL
                     EDISSTFKRTIQQATLYDETGFNVSPFGAGRSADIQFYHLDPGENGMKYQEPEIWDEE
                     RHGQAPPEVVATPTTYVGRENQAYKFSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVN
                     NMAYIQNLEDYENDITTGRMYFPSSSIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQ
                     NPESGEAEPIETSKTTTDGIAIFETKMPILGDETGAVSDIFWIREIAGPPRYQLVDET
                     YQFQIKKYAANEDEMLGHYELVDEYTSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNI
                     EGTEYDELYVKLEMRNEFKPIHMNINKLIEGSNMPINGAEFELYETDGNGLEIGYPVA
                     KGLSGVNEVEEEKGRLTFYSVDEDGKYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAE
                     YKIIESQNPEGFKEPDENDTWILRLYDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWV
                     AFTAYNTFDYREIRVRKLDHEGHTLDGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKR
                     LPGIGYFYSRAVGVEKYVDLNYDQPLHLSIGEYEVSESVAPNGYRLSDQVFQFALNEE
                     GKFIIEDTVIEDETAPLPEGYEIIDGVLQVTLEDELAPIDLELLKIDSMNSRRLEGAE
                     FSIEKQNSAGEYEIIEGGLAPDPNDSSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFI
                     LEISYREEPDIENDRVVPGKEAGTLKVEISYYPNEEAETPDLQQELEYELLETNRIRL
                     QLSVGNDPENPLPATGGSGRLFYILLAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16747..17205)
                     /locus_tag="ACHA5T_001324"
     CDS             complement(16747..17205)
                     /locus_tag="ACHA5T_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8886907.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(17503..18021)
                     /locus_tag="ACHA5T_001325"
     CDS             complement(17503..18021)
                     /locus_tag="ACHA5T_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFM8886908.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(18023..18640)
                     /locus_tag="ACHA5T_001326"
     CDS             complement(18023..18640)
                     /locus_tag="ACHA5T_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFM8886909.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(18637..19491)
                     /locus_tag="ACHA5T_001327"
     CDS             complement(18637..19491)
                     /locus_tag="ACHA5T_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8886910.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(19488..20399)
                     /locus_tag="ACHA5T_001328"
     CDS             complement(19488..20399)
                     /locus_tag="ACHA5T_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8886911.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(20440..21774)
                     /locus_tag="ACHA5T_001329"
     CDS             complement(20440..21774)
                     /locus_tag="ACHA5T_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8886912.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
BASE COUNT         6358 a         4807 c         3775 g         6928 t
ORIGIN      
        1 tcaaatcact ttgttttttt ataaaatttc tgcgcctagt gtggactcaa aatgtctcaa
       61 tgcccattca tgtccaacgg gatcgaaact tgccacggaa tctttgaaaa tatggagttt
      121 gtatcctaga ttatacgcat cgactgctgt atgcaacaca cagatatctg tacatacgcc
      181 ggtcaaataa atatctgtga tctggcgttc tcgcaaacgg atgtctagat ctgttccact
      241 aaaagcggaa taatgccgtt tgtctatcca ataaacattt tcctcttgct catagtcttg
      301 gtataaactt tttaaagagc cgtacaattc ccgtccaccc gttcctatca cattatgcgg
      361 aggaaataag cggttctccg gatgaaagga atcttttgga tcatgcgcat caatggcaaa
      421 aacgacaaaa tccttttgat caataaactt ctttgtatga gcagtcagct ttttttcaat
      481 tacttgtcct gctgtcccag tagtcagctt tccatcctct gcaacaaaat catatgtgta
      541 gtcaatcgag atcaatgcgt tcatcttagt cctccttata cgagatgttt gacaaattcg
      601 atcaccattt ctgctgatcg tttgcctgcg tcttcgataa attcgtcaaa gctttgtgtc
      661 gccgagtgat ctgctgtgtc gctcattgca cgtacgatca aaaatgggat attgaactga
      721 cgagctgttt gtccaacagc ggctccttcc atttcgcaag ctaatgcttc agggaaattc
      781 gttaagattt ctttgatttt atctggcgag tcgacaaatg tatcacctgt aacgatcaat
      841 ccttcttttg cgttcaaatt cgttgctttt gctgctttaa ccatttcact gcgcaaatat
      901 gtgcttgctt cataatatag aggcattcct ggtaattgtc ctgggttgta gccaaatcct
      961 gttgcatctg catcaaaata agctactttg tctgatataa cgatgtcacc tacttgtaag
     1021 ccttcaccga ttccgccagc tgaacctgta ttgatcacca tgttcacgcc gtattgttgg
     1081 atcaacagac ttgtggtaat agatgctaag acttttccta taccggaacg tacgacgatc
     1141 acttcgtggt tacctaatga acctgaaata aataaagcgc ctgcgcgttc ccatgataat
     1201 ggttcgctta ggttctctct caaaattttt acttcttctt ccattgcgcc gataataccg
     1261 attttcatct ttttaattcc ttctttctat ataaatgcga ttgctaaaaa aaccacacat
     1321 aacattagga taacaataat aagagatttc atcaaaaaag agttccattt ggtcgtcttt
     1381 tcccgttcgc tgatccttgt tttcgtgatt ggtttattct ttttgctttc tttacgataa
     1441 aagctggcaa tctttttttc ttcttgctga taagctgttt ctgcttttcg ctgttttttt
     1501 aatgactcgc ttgcttgtgc ttgttgtcgc ttgcgaagtt cgcttcgcgt aataagcggt
     1561 cctttggcca taagccgatc cctctacttt cctttggtca atagttccca gtattgaatc
     1621 gcataaatcg tctttgcatc acaaatcaac tgatctttca tcgcttgttg cgcctcttca
     1681 agagttagat gatacaactc cagtacttcg tcttcatctt gtgctaaagg attctcgacc
     1741 ttctctaccc cttgtgcatg ataaatatga aggacttcat ttgcaaaacc tggtgaaaga
     1801 tacatcgaag taagatggct cagcgacttc gcacgatagc cagtttcttc ttctaattct
     1861 ctagctgctg tcatttcggg attttgtcct tctcctggat cgatttttcc agctggaatc
     1921 tccaaaatca ctttttctaa aggttttcga aattgtttga caagaagcag acgatcttgt
     1981 tcgtcaaaag caatgatccc gacaccgcca ggatgaaata ccagttctct cttcgaagtt
     2041 cctccatctg gcaaacgaac ttcgtctaca gcaacatcaa taatttttcc gctatagatt
     2101 tctttgcgac tgattgtttt ttcttcaaat tgctcattgt tcagcatatc ttctcctgct
     2161 ttctttgtaa tcataactgc ttttttttca tttttcaatt tttctcaaaa actccttatt
     2221 agtttaccat agataaacct tagattttct ctaaatatag aaaatccttc tttagtccga
     2281 tactctttct agtagtccgt atgctaaaat aagtatgtaa tcgaataacg aaaagaattc
     2341 aatactcaac gataaatgac atggagggtt tattatgata aaacgaaatt ggccgtggct
     2401 tctagcattg gtcttgctat ttctctttgt ccgaaacaat agtttttctt ttgttctagg
     2461 acttttattg ataggtgtag ttttagtttt gtggggactc tttggttcgc gcaggaaaaa
     2521 gggaaaacaa gaagaaattc ctgcactgac tgacgaactt gaaactcact atgaaaaaag
     2581 cggtatgaca gcgaatgaaa tttcattttt tcgacaaaca atgaatcaaa caaaaaatga
     2641 gatcaatcaa ttgcaaatga atatgcagca gacagccaaa cttaaagcaa tcgatctccg
     2701 ccatgatcca gtcaaagcag cgaaagcttt attcaaagaa ttggtgaaag aaccaaaccg
     2761 actgcatgaa gcaagtcagt ttctttatac gcatctgccg aatctagttg atttgacaaa
     2821 taagtataat gagatcaatg accatgagat caaaaacaaa cagacctatg aaaaactaga
     2881 agaaagcgcg cagatcatcg accagctgtc agctttaatc gcacaagatt atcaaaaatt
     2941 cgttgctgag gatctagatg atatcgacgt agagatatct gttgcgaaac agagcttaaa
     3001 gcgtgataat aaatatgaac cagaacaaaa ggattaactc ttttgttcat aaaagaagac
     3061 tcggaacatt cagatgatgt ctggctgttt cgagtacttt ttctaaagta gagacattca
     3121 tgtaagaatc gagaacagat tcaacaagga ggaatttttt atggatcata aaccgactga
     3181 aacaaaagat gttacaccag ttaacgatac acttgaagat ttattgaaca acccgttttc
     3241 aactccaaca gatacgctga ccacttcaca acaagcggag attgatcagc tccaagagca
     3301 acagacagct gctcgtttaa ttgacaaact accggcggaa cgccaagaac aagctaaaca
     3361 gctagcagca aaaatcgatg ctaatgatgc acagtctgtc atcagctacg gatcggctgc
     3421 ccaagcaaaa ttaagtgaat tctcacaatc gatgttgaat catgtgcaag cacaagatat
     3481 tggtcctgta ggagattcct taactgaact gatgtatcgt ctccaagaag ccaatcctga
     3541 cgaactgcgt gccggagaag ggaacatctt ccagcgtgtc ttcgggaagg tcaaacaatc
     3601 gatctatgaa gtgacagcaa aatatcaaaa gattggtgca caaattgaca agatttcagt
     3661 gaaattagat aaagaaaaag acggtctgct aaaagataat ctgatgttag aacagcttta
     3721 ccagaaaaac aaagactact ttgatgcact gaatatttat atcgctgctg gagaattgaa
     3781 aatggaagaa cttcaaacaa cgatcattcc agaagcgatg aaaagagcag aagaaactgg
     3841 cgatcagatg gatgttcaga ttgccaatga ttacacgcaa ttcctagatc gtctggataa
     3901 acgtacgcat gatcttcgct tagctagaca gatcacgatc cagcaagcac cacaaatccg
     3961 tttgatccaa aacacaaatc aggccttggc tgaaaagatc caagcatcta tcgctacagc
     4021 cattccgctg tggaaaaacc aagtcgtgat cgcattgacc ttgcttcgtc aaaaagatgc
     4081 agtaactgct caacgccaag tctctgaaac aacaaacgac ttattgaaga aaaattcaga
     4141 aatgctgaaa atctcagcca tcgaaacagc gaaagaaaat gaacgtggaa tcgtggatat
     4201 cgagaccctt caaaccactc aaaatgattt gatcgaaaca atccaagaga cattgcgtat
     4261 ccaaaaagaa ggaaaagaaa aacgtcgtca tgcagaagta gagcttggac acatggaaga
     4321 agatctgaaa caaaaactgt tggatttaac ccaatagatc acctttcgct tgttactaaa
     4381 acaaacgccc aacaacagtt tattacaact gttgatgggc gtttttctta tctttcttct
     4441 aacttctcca ttggagtcta tttctcattc atcttgctgt gtccatagcc atagccaaaa
     4501 taaatgatca gtccaataac cattacgata ccgaacacga tccatgtcac agtttgcaga
     4561 cgaagcatca atacaataca agtcaaaatg gagacgattg gcaatagcgg aacgaatggc
     4621 actttgaatt ctccagcttt tggtttgcca aacatttttc tcaacttcag taagcctaga
     4681 ctaacaagca tcaaataggc gagcgccatg atattcgtta gttccgctaa tacattcaat
     4741 gggaaaaatc cggcaaagaa tacagctgcc acaccagcgg cataagtcgc ttttttcggt
     4801 gtacgatgct tttgatcgat ttctgtcata aacttaggaa gtaagccatc cttgctgaca
     4861 gcaaacagta aacgagcaag tgaatacatc atcgagatcg ttaccgtcag tagcgttaaa
     4921 attgccccta cagcgatcac cgcgcccact gttccatgtc ctacatacct cattgcaaaa
     4981 gccactgggt cattgactcc tagttcagta tacggaacga ttcctgtcaa gatcagcgtc
     5041 acgatgatgt aaaggacagt agcaatcagt atcgaaccaa tgattcctct tggcacatct
     5101 ttttgcgggt ttttgacttc ctcggctgcc attgcaacgg catcaaatcc caagaaagca
     5161 aaaaacacaa gagctgcacc atctagcaca ccggaaaaac caaaaggcat gaatggctgc
     5221 cagttatcag gtttaacgaa aaatattcct acaatgataa acaacgcgat gataccaaat
     5281 ttgacatata ccatggcatt attcaaacgt aatgctttct tggcttcaag cgacacgatg
     5341 taagtgacga atatcactac gagcaccgca atcaagtcaa tgtatgtacc attttccgga
     5401 ttgtacccgc cagttaatgc ttgtggaagt tcggccccta aacttttcaa aaaaccttgc
     5461 atatagccag accatccgga agcaacagag gaaaccgcta gtaaaaattc cccgatcaac
     5521 aaccaccctg tcagccaggc agcaaattca ccaaatacaa catacagata ggcatatggc
     5581 ccaccaatca ctggcactcg cgaagcaaat tctgcgaaac ttagtccaga caaaatgaca
     5641 acgattgcag ctaagacaaa tgaaagtgac agtgctggac cagcattttg attggccgca
     5701 acccctgtca ccacaaatat tcccgtaccg atgatcgcac caattccaag catgatcaaa
     5761 tcagacgttt ttagttcttt tttcatctca ctcgtatgat tgagattgat ctctttctta
     5821 cgaaacaaat ccattgaagt tcctccccat tttaggttgg aaaacagcga ttaaactgtt
     5881 ttcatatacc ctaaaaggat accacctaaa atgactaaaa ggcaaccaac aataacataa
     5941 atgagctctt ttttcgtttt tctttcgcca aggatgaaaa tcccacctaa agtagagata
     6001 atgatcccca tttgtgaaag tgagaagcca acagctaaac caagtgattt gactgttaga
     6061 agcatgcaga tgtttcctaa gccccataat aatccacaga tcatgttttt ccacacaaac
     6121 tgatcgactt taactttacg gaaagcaaag aagcttgcgc ctaagatcat tccgatactt
     6181 tgaggtaaga taattgacat cgcatccaaa ttcgcccagt tgacgatgat ggtatataca
     6241 ccataaccaa tcgtagagaa gatcaatgct ctaaaaccct taccaaaatc aagcatttta
     6301 ttatctgtat ctatttttcc ttcgtcatct tgtcgggcag tcaaatacgc accgcttaca
     6361 agaagagcta aagcaataat accgtataca taatctttcg tttgtgtcca ttcatggaag
     6421 aaaacggctc ccgcaatcgt attcaaaatt aattgaaaac cagtagacaa tggtagccct
     6481 acagaaacac ccatgtactt cattccatgg aactgtccat tttgtccaac actccaagct
     6541 aagccggata aaaatccgat caagaaaatc caaggggtga ttacaggact aacaacgaaa
     6601 aagacaacta aagaaaacag aaatgcaccg atcgtcattc ctagtgtttg ttgattggca
     6661 tcgccaccaa ttttaccact tactaaacca atactacccc atgccaacat agggactaat
     6721 gctattaaaa tttccataac caatctcctt ttatttcaca tgataagaaa cttatcagcg
     6781 aaaacgttaa caacacaagc actttttttc gtttctaacg attttctaac gtttttcatt
     6841 ataaaaattt aagattggga aaaataaagt cattgttgtc ccttttcctt cagttgattc
     6901 agccgtcaaa tggataccta atttttcggc cagatttttt gctaaataaa gtcctaatcc
     6961 tgttgaatgt gttttgctaa gtcgaccatt tctcccagta aatcccttat caaagatccg
     7021 tttcaagtct tccgctggga taccgatccc tgaatcttta actgaaaggc tgactccttg
     7081 cgcttccttt tctatgctca caatgatgtt tccgtgatcc ggcgtatatt tgatggcatt
     7141 actaagcaat tggttgaaga taaaagcgat ccatttagca tcggtcaaaa cagtatgatc
     7201 ctcaccaacg atctcgtagc gaatattttt ttggataaaa taatttgcct gactgcgtat
     7261 cacaggctga atgattcctt ttagtccata ttcttggatg aggtagtccc gcgagaaact
     7321 gtcgagtctt gcataataca aaacttgttc tacgtattca tcgatttttg ataattcatt
     7381 ttctagtaat gtatactttt gatcgtcaat atcgaactcg atcgaacgaa ctagcaattt
     7441 agaagcggaa agtggcactt tgatttcatg gacccatgag tcaatataat ctttttggtc
     7501 ttgttgatta ctgattgctg attgcattac ttcttggtgt tcacggatca gctgattgat
     7561 atattcttct accagttttt cttcttctgt cctcccacca ctcaaataat tttgcaaagg
     7621 ggaagttttt tgtatgtcgg ctaattttcc ccaccatctt ctcttgctca agtaaccacc
     7681 cagcagataa aaaatcagaa agacgccctc aatcaaagct aaataagcaa ttgtgctcaa
     7741 atcaactttg atgtttggcg ccagccacat cacaaagatc gtcaatgcaa taaaaaacag
     7801 ccatcccaag atcaatgtcc actgatcttt caaatattta aaaaaattca tcttaaccgc
     7861 tcatcctttc agggaacgat atatccctgt cctacttttg tttcgatata tccttggata
     7921 cctgcctgtt cgatttttcg gcgtaaccga ttgatgttga ctgtcaatgt attgtcatct
     7981 acaaatcgtt cgtcatccca cagtgctcgc agcaattttt cccgacttaa gatctttcca
     8041 ttgtgcttca tcaaaataaa taatagacga tattcatttt tgctcaaatc aatgatctct
     8101 ccgttgattt ccatacttcc gttgtcaaca ttcaacgtaa tgccattgtg gctcatcatt
     8161 tcactagata attctgtgta gttgtaagaa cggcgaagta atgcattgat ttttgcaatc
     8221 agtacatcga tttggaaatg ttttgtcaca aaatcatctg cccccatgtt catagccatg
     8281 atcatatcca tattcgtatt cctgctggag ataaaaatga tcggtacttt cgatacttca
     8341 cgtatttttt gacaccaata atagccatcc aaaacgggaa gattaatatc tagtaataca
     8401 agctgtggtt cttctctttc aaagtctgag aacacttgat tgaaatcggt cgttccaaag
     8461 gtttcaaatt gccatttctc aagttcctcc atgatcaatt gtcgaatgat ctcttcgtct
     8521 tcgactacca tgatttttgc cataaaaaat ccctccatct ttcttcttgc tactacaata
     8581 tcaaagaagc ccttgtgaaa caagttggct tacctggaaa atgtcatttt agcaaacgtt
     8641 aaaaaaaact ggaaatatgc gatttaatcg cgtttgtctc ttccaagcag cttaaattta
     8701 ttatgttatt cttaataatt ggtctattat ctgttatttt tctttcgttg tttttgtctt
     8761 ttaaattttc gataacacca ataaccgaaa agcgttagca ctagcaatat acctagaagg
     8821 agtataagta atcgataaag atggtatttt tttgtctgtt ctatctttga agagacttct
     8881 tctgtaaagg gaactcgtct tcctgtcact agtaatcggt gagtgttcac ggtgtaaggt
     8941 gtacaagtga taagtgtgac tagatcctgc ttttctctaa ttgctaatgt atttgttttt
     9001 gttggaagga caacaatttt ctcaacgact tcataagcga gcatccgacc atacacttca
     9061 agatagaatc ggtcatcttg ctccatttct tcaagatccg taaagagttt tttctcagca
     9121 ataccgctat gggcggtaat cacggaatgt gtactctctc ctccagttgg aaaagaggtg
     9181 ccttgcagga ctgtcgctcc tctatctaac aaaagatcat tcgtttcatc aaataaaggc
     9241 aagctaacat tgattttcgg aatatagatg gctccgatca tatgttgttc gtaatattct
     9301 tttcctggat tttggacatt tctaaccgct tgctcaaaag gatctttgac ttgtcccatt
     9361 cctgggatta ctgatgtatg agcaagcgtc tgatttttct tttcatgcac tgctaattgt
     9421 tgctcatgtt tttcttcttt atcgcgttca agctgctttt gataattctc gatccttctt
     9481 tgatccaaat aattgctgag agcatctgct aaaaatggat atgtaaaaac tgcagctccg
     9541 gcaagaaaca tcactgccat aaataattgt aataaaagac tgatttgggt ttgaggtcta
     9601 ttacctattt tctttttcat tttcttcacc ttcttattta aaaaaactgc agcaaccttt
     9661 ataaaaccga ttgctgcagc ttatttttgt tgcttatact tcagacttca ttttagattt
     9721 acggtaccag ctgtacgctc caatcatcaa acttattccg attagtaaga atgccaagag
     9781 cccgttccca ccagtagcag gaagtgaccc gcgtttcata ttcggtactc tttctggccc
     9841 tggattttct actcctacga tctgaatttc ttcattatag cttccataag agatgataaa
     9901 ttcagtaaag gctgcctcac ctgttggaag tacataacct tctggcgcca tggtctcttt
     9961 catttgatag gttccataat ctaacccttg aacctcaaag tatccatttc catctgcgac
    10021 aaattctgtt gcttgtccgc ttgttgccca ctcaattcta tctgctcctg attcataaac
    10081 agctaaaggc gactcccctt tataaaatat tgcgtattca gaacggtctt cattccacaa
    10141 ttcgaatctt gcatctttaa gcaattcact gcttgatgca tcatattttt caaactttct
    10201 tccacctgtt gtgactttct ctggcggctc tactccttct tcataatcaa attcatcatt
    10261 tcgtccgatt tcaataaccg ctgtgttgtt atggaaatca tccgcttcta gttctgtgga
    10321 aaccttcaaa ttatacgtga tcgttaaatc tcctccacgt gcatctgcta atctacctaa
    10381 agtatctact ttggctactc cattttcttc tgtcaaaatc gtcacgacaa acccgttgct
    10441 tactacttct actatataat cttgtccttt tgttaaaatc gtctcgccgg ctcgcacttc
    10501 tatttcctga tcataataag ttagaccaac tgtaggcgta tctgtgattt tgtaattttt
    10561 gataacagcg cttccgtttt caaggacaga atcgatgaaa taaggaatag gtgctgtgat
    10621 cgtgtaagaa gccgtttttc ctctttctag atcaatataa gaaatcgttt cttgttctcc
    10681 agcttcatta ataatcgtta cttgtctaag gtcgctttcc tctacattca tctgtttatc
    10741 tgcttctctg atttcgtttt tcggatacaa atgcacatct gtattgtatg tgtctttcca
    10801 tgtagaacca tcccacacgc catccaccat atcaggcatc atcactggca tcataacaac
    10861 cattggagac gcgattctgg aaatatgagc tggcgaataa gtttctaaga aaagatagac
    10921 tttatctcta gaagaaggtg cttcttccgt cacttggacg gtagagaata ccgcttctcc
    10981 agcttcattc gttgtttgtt tgtcaatact tgtcagtcga tattttaaaa accaactttc
    11041 tgtgtgccgt ttttgaatcc attcaatcgc ttcggcagaa ttaagaccgt cttctcgttc
    11101 cggtgttaaa ggattatctc gaatcaaatc gtaatatact tccgttacgt caaacaaatc
    11161 aaaatcgact cctggaatat tttcttcacc aaaatccgta gaaaccaatc cgctattcgg
    11221 tcgttcttcc ggaacagatg agaaaccttt tttgtgtaat gtaatcgtca cttcacttgg
    11281 tttttgtgcg acttcttcgg ctttgactag ttttccgttc ccgaaaatcc ctattcccgc
    11341 agtaacgagt aacagtacac tcattcccgt gacaatcatt tttttattca tttttttctc
    11401 cccgttcttt ttttattaat aaaacagcac ctaacaataa aagaattcct agtaaagaaa
    11461 tcaacgttct tgcttcattc gtttgcggga atacggacca ttttcctgat tgacctggat
    11521 tgcttggacc actcggctct ttcggtttgt ccgttgtttc ttttacggtc tcgttataaa
    11581 cacgtggtgt tgcggattca taggcagaga ctggaacttt cccggattct aattcagcca
    11641 tctcctgtcc gttaactact acatattgtg gttggccttt tgcatccacc caaaattcag
    11701 ggatgatgac ctcgattctc ttacttgctt catccatctc gtaaccttcc acacctttta
    11761 attcttcaaa ataataggtg ccagaaggca agaaacgttg tcctgtcgta acaaggccct
    11821 ctttatccga aatgaatttg cttacatttt tgtcattcaa aggatcattg tctgctggtt
    11881 tgatccattg atttttcaag tcattagcag gagccatatc taaataatat ttcccccctt
    11941 ctatcatctg atatagagca aaaacagcac cttctaatgg ttttcctttt tcagttgttc
    12001 ctctttgacg tccatacttg aagaagtatg gatctcttag ataaccgaca tttttaggat
    12061 agatatgaat ctcttgcagt tcctcggttg attctgtcgg gtgataaatt ggcaaaatag
    12121 cagcaattgg gatagctgtc tgatcgatat caacatccaa tccaacttct ggatcaatcg
    12181 attcttcgaa aatcaaataa actgcatccc gatcttggtt tttcttaggt gctttgatac
    12241 gtgcaatccc gtctcctaat ccgccaaccg tatctttccc agttgttacc gaagtcccta
    12301 tttggacaag cctttcttct gtgattagct gacggatatt gtttctgttc gtttcagcga
    12361 tttgctggac gaattcttta ggagtcatgt tttcagcagc ttttttttca tgataataat
    12421 cggtcatgtc gtaaatggca aatgtcgctc catttagtgg caccgtctgt gaaatgatat
    12481 ctgctgtttc ccctgcttcc tcagagatag ccaagccatc attttcatgg tattcaaatt
    12541 gatcgttata atcgatatct cgaatcatcc gtttatgcaa cacgaattcc acgacatctt
    12601 cccctgattc ttctgccgac atcgcatgtg gaagaaggac tgctgcaagc ggtagtaaaa
    12661 caagtaattg aagcatcaaa cacaacagct ttttcattac cttgctccct cctttttaga
    12721 ttgtctgaca aacaaaccat aggctgctcc tgcaagcgcc atgaagaatg cagcaagcag
    12781 gatatagaat aatcgtccgg atcctcctgt agcaggcaac ggattttcag gatcgttccc
    12841 gacactaagc tgtaatctga ttcgatttgt ctccaaaagt tcatattcta actcttgctg
    12901 tagatcaggc gtctctgctt cttcgtttgg ataatacgaa atctcaactt ttagtgtacc
    12961 cgcttctttt cctggaacta cccgatcatt ttctatgtca ggttcttctc gataactaat
    13021 ttctaaaata aagtaacctg gaagttttct atagctgtca ggtgacttca cttccttgat
    13081 ccgatagacc ccttccaaca agtctgaagc attgaatagg gaagaatcgt ttggatctgg
    13141 tgccaagcct ccctcgatga tttcatactc tcctgcacta ttttgtttct caattgaaaa
    13201 ttctgcgcct tcaagacgtc ggctattcat actgtcaatt ttcaaaagtt ccaaatcgat
    13261 tggcgctagt tcatcttcta aggttacttg tagtacacca tcgatgattt catagccttc
    13321 cggcaaggga gcagtttcgt cttcgatgac cgtatcttca atgataaact taccttcttc
    13381 attcagtgca aactggaaca cttgatcaga tagccgatac ccatttggtg cgacagattc
    13441 cgagacctca tattcaccaa tagacagatg cagaggttgg tcgtaattta aatcgacata
    13501 cttttctact cctacggcac gagaatagaa gtatccgatc ccaggcaaac gtttcgattc
    13561 ttctggttct aacggattac cagtgtatgc tcgatacagc gggaaggaag aatcccccac
    13621 tttcctgata tcgaaacctg caccatctaa tgtatggcct tcatggtcta attttctaac
    13681 tcggatctcc cgataatcaa acgtattgta agcagtgaat gctacccata aacgatcctc
    13741 ttcatctgtt gtatacgtga gatattgcca attttcttca cctgtttgct tgagttggat
    13801 agtccctata gcattatcgt aaagtcgcaa gatccatgtg tcattttcat caggttcttt
    13861 gaaaccttct gggttctggc tctcaatgat tttgtattct gcatagttgg tagtttcttc
    13921 actatcctta taaacaaatg aatctggctc tccgattgga tgaataactt tctcaccatc
    13981 cacgattaca tatttcccgt cttcatctac tgaatagaac gtgagtctcc ctttctcttc
    14041 ctctacttcg ttcacaccag aaagcccttt cgctactgga tatccgattt ccagtccgtt
    14101 accatctgtt tcataaagtt caaactctgc gccattgatt ggcatattac tgccttcaat
    14161 tagtttattg atattcatgt ggattggctt gaattcattt cgcatttcca gtttgacata
    14221 caactcatca tactctgtgc cttctatgtt aggctcttgt acaacagaaa gcttgtgata
    14281 ttctccttca taataggaat tcggatcttt accttccggt ccgccgactc tcgaaccaga
    14341 tgtatattca tctacaagct cgtagtgacc taacatctca tcttcgttgg ctgcatattt
    14401 tttgatttgg aattgatacg tctcatctac taactgataa cgtggtggtc cagcgatctc
    14461 tctgatccaa aatatgtcgc tgactgctcc agtttcatct cctaaaatcg gcatcttcgt
    14521 ttcaaagatc gctattccgt ccgtggtagt tttacttgtt tcgattggtt cggcttcccc
    14581 actctctgga ttttggtcat ataatgcaaa ttcgactcca gccaacggct cgccattttg
    14641 acccttcttg ctgacttgga aattacggcc tttatgttcg taacgaatgg aagaagatgg
    14701 gaaatacatc cgtccagtag tgatatcatt ctcataatcc tccaggttct gaatataggc
    14761 catattgttc actgtgcaaa aatttccgtc ttgagctccc gcatcaagtt ttacttggta
    14821 cttgatcctg accatttccc cttcacctaa agaaatcgga gaaaatttat aagcttggtt
    14881 ttcccgtccg acatacgttg tcggagtcgc cactacttct ggtggggctt gtccatgacg
    14941 ttcttcatcc catatttctg gctcttggta cttcatgccg ttttctcctg gatctagatg
    15001 gtaaaactga atatctgccg atctgccagc tccaaatggt gagacattaa aaccagtttc
    15061 atcataaagt gtggcttgct ggatcgttct cttgaaggta gaagagatat cctccaaggc
    15121 tgtaacaatg tcattcttgt tgttcgcatt gtaatatcgc ggtgttacgc catcttcttc
    15181 taaatcagca atatttttca atacatttcg tccaatagca tctagtcgtt gtctcccgct
    15241 aacgtttcca tcgattccta aaccaatgga gaagatactt ggcgggttct cttgatactt
    15301 gtttttcagc caatgatgat aagcaactgt gttgatcgca gatgactttt cttgcgaagt
    15361 aggactgcca ttcgtattgt tattagaaga accatcacca acccagtgag tcgctttatt
    15421 atctccttct tttacctctc cataacgata gtgtacattt acttcatctt ctggacggtt
    15481 aaagtcagat gcataggtaa ctggatgagt aaaccctgca ttagaagtat tcccagaaaa
    15541 ctcctcaaaa tttctgaaca aaacgtcatt atcttcagca atcttatctt ccatcacagt
    15601 ccagttgctc caagctccat tagtgctcgt acgataactt gactgaatcg gtgcataact
    15661 gaaggtcggt gtcccatctc ccacaacgat taggattttt tctggatttc tattttctcc
    15721 gccattgtct ttatacaatg tttcatagcc atttttaagc cctagtgtaa gcggagttcc
    15781 tccgattggt gtacgattta atgctgtatt ctcacttccc gtcaaacgat tgatatcatt
    15841 tgtcaatgga aagatgtccg aactgatttg ctcatggtta ttgatagctg tactattatg
    15901 gtaaaaattc accataccga ttcgaatatc cagactcggg tcagagagca gatctttact
    15961 catttcgttt actgcctcaa tcaacgcagc atttttagtt tgactattgg tcccagcggt
    16021 taattcactc atactcgctg acttatctag aacaaaaacg atatcgattg gactgatttc
    16081 ctttttctcg cccccgatga catctagata gacattgtaa agggttgttg catcttcagg
    16141 atgtagatca gatgctgtgc tgcttggcag aacaggttct acccattttt tgatgtacgc
    16201 cccgttataa tccaagtaac tgttttcaaa attccgttca tttccccaaa gctgaacggt
    16261 ttgagctgga acgtttctat cttcttcgcc tgcagttgat ccattattag tagcatagtc
    16321 gtaatttcgc cagttcgctc ccccgtttgt ataatcacta tcatcgatca agttctctcc
    16381 gtcatatggc gagcctttgt ctccttgata gcgttcattg ctttgtgtcc aatttcctaa
    16441 cacttttgta tagtcattcg ttgtaaactt cgggtagcga tgctgttcgt tttcaacttc
    16501 tccgctagga tagatatatt cataggggtc ttgcgtagct gttggcccaa gacgctccaa
    16561 aataccgttg aataactggc gttgaatgcg ctggagtgct tgaccgctgg gtacgattgg
    16621 ttcgtccagc ttttccatct cattttcttc gctacgttga atttcagcgg aatcttctgt
    16681 atcagtttca ttttcttgta attcttggtt atctattctt tcatcaacca ccacttgaag
    16741 aggtagttac ccttcgttca catcggggat cttttctgtt tcaaaaatca atgtgccggt
    16801 ttctgcttct tttgaataaa cttttccgac ataccattct tgaccatcga tcgtctcgac
    16861 ttttaggtct tctccatctt catcatagga ggatagacct gttccacgaa cgttttttac
    16921 ggtaccaatc ccggcggctg ccgcatcgat tgctaaacgt attctacctt cattggcact
    16981 ttcctttttt tcaaaagcca gatgccattc aagggtttct tctttttcaa tatacttccc
    17041 ttcaattttt gcatagtcct catctaagaa tacctcatcc tcctttgttt ctgttgactc
    17101 agcagctaca tccagggtcg tcatatgata gactgactgg aaatatggtc caaatagcaa
    17161 actgacagtt actaactgca aaagcttcaa ttttaaccct ttcacatcct tatactcctt
    17221 tcaaaaatat ttgtcttctt tcacaacaat caacaaaaca atgcccatct aattataatt
    17281 taatttaatt ttaattttaa atcaaatgat atccttttta gttaaaaaac accaattata
    17341 caacatttat tattaaaatt aaattataaa attttataac gttctcattt aacaaaactt
    17401 aagttattaa gacaaatgaa taaacaacta cgctttcctc gaaacaaaaa aataaagcac
    17461 aaatgcgcat ttacgcactc atgctttatt cgtacaatta attcagataa cggcagcgca
    17521 taggatgcca ataacacttc ctaaaatagc tcctcctaaa atgtcagtag gataatgtaa
    17581 aagtaaaaaa atcctcgaat aagcgatcaa acaagcaaat aaaaaggcta atattcctgc
    17641 aactggactt aattcgaaca acgcagctac tgataaaaag gctagcgctg tatgtcctga
    17701 aggaaaagaa gaagtcttcg gtttacttgt gtaaaggaca agccgacgat ttttttcata
    17761 aggtcttttc cgtcttgtcg tttttttgat aaaaagcaaa ccaacaaaaa aatagatcaa
    17821 actgccgata agaatggcta gaccatattc tctagtatca ggtatcaaga ctaaaacagc
    17881 agcaaggagc aaccaaataa aaccaccatt agagatatta ctgataaatt tcaaaaaatt
    17941 gatttttcga ctatccgttt tccgttgctt ggcgaacttg ttgataatac tatcatcgat
    18001 tttagcaatc aaacgcaaca tgttattcct cctttttaag aaccttttct tgcgtacgat
    18061 ggataaaaag caggacttga aaataaaaca agaaactgaa acctaataaa gcaaaataga
    18121 ttggtcctgc aaaccagaat gcgacaagaa ggatcaacgt gcctgccaaa tagaaaagcg
    18181 cgcttttgaa atcattaaaa aacaagaaaa atgcagtaat ccaagaagct tctttttgtt
    18241 ccagttgaag caagattttg taactaaaga gaagcgcgct taatagaagc aaaaacaacg
    18301 tagtatacat caatgtatag ccaattcccc caccccaata gcggaaaatc tgataatcga
    18361 tcaatagaag aagaaaataa acacttgcta aactatcttg ctttccataa agagagaagt
    18421 tctcacgaaa ctttttccag aatagtgaac cgagcttttt ctcttcattt tttttgtaat
    18481 gataaatcat atacagaact gttcgacttg cagttcctaa tgtgaaaaca ggcaagacgc
    18541 acaggaacca tagtagctgt aaatggataa gacgtggaat ccattcaatg atttctaaga
    18601 aataatcatt ggctttttta ccaatcgatc gattcatcat ccttttgaac ctcctgtcaa
    18661 atccatccct tcaatgaaat aacgttgtgc aaaaaagaac agcaaaatcg tcgggatcaa
    18721 gacaagtgtt gctcctgcca tcagataatt ccattgtgtc gttgcttggt tctgaaaaac
    18781 ttgcaagcca atctgtaacg tgtatttttc ttgatcttgg atatataaca gaggacctaa
    18841 gaaatcattc caagctgcgt tgaaggaatt gatcgcaatg gtaatcaagg caggtttcgt
    18901 caatggaagc atcaaatgac tccaaatata caaatgattc gctccgtcga tttcagcagc
    18961 ttcgatcaat tcattattga tcgataagta aaattgacgc atcagaaata tattgaacgc
    19021 attgccgaag aacgatggaa cgatcagggg caaataagta cctacccagc cgatcttgga
    19081 gaataataca tattgaggga tcatcgttac aaaccccggg atcatcatcg tagaaagaac
    19141 caaagcaaac atcagctttt tcccaggaaa atcaagcttc gcaaatccat aagcaataaa
    19201 agaatttgaa aggacattac cgatcactac tagcacagtg atgaataatg tatttcttgc
    19261 atagcgccaa aaaggaaagg cggcaatcgt atccagatag ttttcccata cgattttttc
    19321 tgggaaaaag gtcggaggat aacgaacgat ttcttccatt ggtttcaatg aagtaaacac
    19381 catccaaagc aatggaatga tcaaaatgat tcctattact gtcatgagga cataacttag
    19441 tatttttttt gtttgccgat tcacactggt ctttttgaaa taatccatca tttgccgtca
    19501 cctccagaat aatagaccca cttaggtgca agcttcaaat tgacaaacgt cagaaccaac
    19561 acgatcagaa acaggatcat tgacatagcc atcgcataac ccatatcaaa agctacgaac
    19621 gccttgttcc acatatgcag attataaaaa agcaaagaat ttgcaggtcc gcctgttcca
    19681 tttttcgtca tgacataagc ttcttgaaaa atttggaaag aaccgatcgt agaggtgatc
    19741 acatcgtaaa aaataatagg agtgattaat ggcaaagtaa tacttttgaa ctttccccaa
    19801 acgcctgctc catcgatttc tgctgattca tataaagatc taggaacatt ttgtaatgta
    19861 gctaagtaaa gtagcattgc tcctccagaa ctccatattt tcatcaaaat caacgcaggt
    19921 ttcgtccatt gtggatcaaa cagccaagct ggtccaggga tatggaacca gcccagaaat
    19981 gtgttgatca atcctgcaga tggagaaagc agctgcatcc ataagatgta gactgctact
    20041 cctgataaga ctgccggtaa atagaaagct gtacggaata cactgattcc tttgattttt
    20101 tgattgagta ctgtggctaa aaacacactg aaaatcgtcg tcagcggtac attgaagatg
    20161 acgaaataaa cggtgttgta taacgatttc caaaataaat catcgtgaac gaacattctt
    20221 ttgaaatttg ctagaccaat gaaattcatc ttggatgtca aattataatc ggtgaaacta
    20281 ccaataactg aaaaaagcat aggccctagc ataaatacca agaacccgat aataaaaggc
    20341 gagataaata aataggccca tcttgcttct tttttctttc ggatagataa acgcttcaaa
    20401 ttttcttgcc tcctctgtac ctagacagtc aaaaatggat tattgatttt gcttgactaa
    20461 gttttctact gctttttgtg catctgccaa tccttctttt ggtgattttg ttcctaaaag
    20521 aatctggtca atctgttcat tcactaatgt cgtgtaatcc ggagcatcta aaggaactgg
    20581 tgtgatcacc gtatttttaa agttttcgtc cgccatttga tagatcattt ttcctttatc
    20641 cgttaggttg tcattattca ctaaattctc attggctgtc ttgctcgcca tgatatcaaa
    20701 acttttttcg ccgaattttt cttgtacttc tggagaagaa agatatttga tgaattcata
    20761 agaggcctca ggatcttttg ctccgtatgg tacttccaag acgaatccgc caccccacga
    20821 ccaatggcct gaaccttctt ctttttctgg gagctgcatc acgccaaaat caaaatcatc
    20881 cggtgcattt tccatcaaac tagagtaata attgatattt tgggcacgca tggctactaa
    20941 tccggaaata aatggatcag atacaccaga cccaaattcg gcttctaatt tattgatcgt
    21001 atttttccca taataatctt gccagtcttg gatccactcc aaggcttcca ctttattttt
    21061 agtatcaatc ttgacttttt catctttgtc aaaccaacta gtcccgttat ctgcgtttag
    21121 cgcccataca tcagccccta aattccataa tggataaaaa ccgatgcgtg taaattcttt
    21181 tccttctttt ttatctagtt tacgtgctgt ttcttctaat tcatcccaag ttgtcggtag
    21241 atcttgttca ctgattccag cttctttaaa taacttttta ttataaaaaa tgacttgtgt
    21301 atctgtattg aatgggatcg cataaggatc accttcatat tctactgtat cccaaagctg
    21361 cggataaaaa tccttagaag tgtctttttc tacatatttg gagagattag ttgcttgctg
    21421 tgcttctgct ctttgggcaa cggaatttat gtcctgtaca ataacatcgg gtgcatttcc
    21481 agctgttatt gcagataaag atttagtcca gatgtcaccc catggctgat acttataatt
    21541 tacatggatc tcgttttgtg attgattgaa atcctcaatg atttcttcga tagcttcttt
    21601 tcgtgcaccg gatccccaaa aggaccagaa atcaatattt tttcttccat cacttgtttt
    21661 ttcagaagaa tttgctgcac ttgaactggc atcgcttgca ttacttgatc cgcatcctcc
    21721 taataaaaac aatgctgaac ttgccagcaa ccctgtaaac aataattttt tcatatttta
    21781 cctccaaatt ttaatgtaac cgatgccggt tttaatataa cgatatcatt ttgttctgaa
    21841 aataacaatt tataaggtat gattctaa
//
LOCUS       DAYYGO010000043        21756 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000043 DAYYGO010000000
VERSION     DAYYGO010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.043"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..3660
                     /locus_tag="ACHA5T_001330"
     CDS             133..3660
                     /locus_tag="ACHA5T_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFM8886913.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="ACHA5T_001331"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="ACHA5T_001331"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFM8886914.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="ACHA5T_001332"
     CDS             7448..7789
                     /locus_tag="ACHA5T_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFM8886915.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="ACHA5T_001333"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="ACHA5T_001333"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFM8886916.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /gene="pheT"
                     /locus_tag="ACHA5T_001334"
     CDS             9146..11566
                     /gene="pheT"
                     /locus_tag="ACHA5T_001334"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFM8886917.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACHA5T_001335"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACHA5T_001335"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFM8886918.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="ACHA5T_001336"
     CDS             13000..13737
                     /locus_tag="ACHA5T_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8886919.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="ACHA5T_001337"
     CDS             13751..14566
                     /locus_tag="ACHA5T_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFM8886920.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="ACHA5T_001338"
     CDS             14579..15226
                     /locus_tag="ACHA5T_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8886921.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="ACHA5T_001339"
     CDS             15242..15898
                     /locus_tag="ACHA5T_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8886922.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="ACHA5T_001340"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="ACHA5T_001340"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFM8886923.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="ACHA5T_001341"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="ACHA5T_001341"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFM8886924.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="ACHA5T_001342"
     CDS             18240..18758
                     /locus_tag="ACHA5T_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8886925.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /gene="cbpB"
                     /locus_tag="ACHA5T_001343"
     CDS             18865..19365
                     /gene="cbpB"
                     /locus_tag="ACHA5T_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFM8886926.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19645..20565)
                     /locus_tag="ACHA5T_001344"
     CDS             complement(19645..20565)
                     /locus_tag="ACHA5T_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFM8886927.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20691..21743
                     /locus_tag="ACHA5T_001345"
     CDS             20691..21743
                     /locus_tag="ACHA5T_001345"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFM8886928.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
BASE COUNT         7282 a         3768 c         4654 g         6052 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag gaattcacga aaattgcttt
    19501 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgccgtttat
    19561 acgtgtttag agcgtgggac aaaagtgttc tttacttttg tcccacgctc tttttagtga
    19621 gattcgttgc atcgtttgtc caaattattt tccgacagca attggactgt tcggaatcgt
    19681 gaaaccagaa gctgtcaatt ctttgacata ggagctcaag aactcttctt ttaaggcata
    19741 ttgttttccg ttcaatgcgt agcagaccgt acgaatcgca aaattgctgt ttccaagatc
    19801 cactaatcca aagatagaag gttcagtttg gagctcttct tggtattttt cagctaatgt
    19861 acgatttact cgatcaatga tctcatagat tttgtcatat ccttcttcag gaacaatccg
    19921 aatatctaaa agtacttgca tatttgcacg agagagattg ctgatcgtgg tgatgtttcg
    19981 attcgggatg aaatggacag taccatccgt tgctttcagc tgaagggtac ggataccaac
    20041 agatacgacg gttccttcaa tcgttaagtt tgctagcttg atgtagtccc caacatccat
    20101 ctgttgttcc atgataataa aaaatccggt aataacatca ctcatgaacc cttgggcacc
    20161 aagaccgatt gccacacctg caattccggc accagccaaa agtgatccga tcggcacacc
    20221 gatagcagat aaaattgcat agataaaaaa gaaaccaagg gtataatgaa aaatagtatt
    20281 taaaagacta tgtaaagtct ttaagcggct ctcgtttaga atggcttttt tagaatactg
    20341 attaaaagac ttatcaatca gatattttcc cacacggtga agaatccaaa aaaggacgat
    20401 caaaaaaatc agatataaac ttttttggat aaaggcagcc aaaatggcgt cccaatcaat
    20461 cccgtcccaa aaccgctgaa acgcatttac tttgctgaca gcttgatctg tcaatgtctc
    20521 gccagaagag tctgtcgtac cggcagttgc taataatgaa aacataaaat cctcttttct
    20581 aaaatttgat tctttctgta ttgtaccaag aagttttcaa aaaaggaaat agcaaaaata
    20641 actataatga aggaaaaaag ctgaaaataa aatgacaaat aatatggata atgataaaat
    20701 gtggagaaaa taacgaaggt ggtaagtata tgcaaatcga ttggaacaat ctaggttttt
    20761 cttatatcaa aactccttgg cgttttattg ccaaatggaa agacggacaa tgggaagaag
    20821 gagaattgac agaagataat tacctgacga ttcatgaagg ctctccagcg cttcattatg
    20881 gtcagcaatg ttttgaaggt ctaaaagctt atcgtcgaaa agacggcaag gtcaatctct
    20941 ttcgacctga acaaaatagt cgacggatga atcaaagtgc tagacgattg ctgatgccaa
    21001 tggtccctga aaagatgttc gttgatgctg tcaagcaagt ggtaaaagca aatgaagatt
    21061 ttgtgccgcc ctatggaaca ggcgctacat tatacttgcg ccctttactg attggggtcg
    21121 gtgaaaacat tggtgtccat ccagctccag aatatttatt tgtcgtcttc tgtacgccag
    21181 taggagcgta tttcaaaggt ggactaaaac caacgaattt tattgtatct gattacgatc
    21241 gtgctgctcc taaggggact ggtgcagcaa aagtcggcgg aaattacgca gcgagtttac
    21301 ttccaggaac agaagcaaaa gaactggatt actcagattg tgtttatctt gatccagcca
    21361 cacatacgaa aatcgaagaa gtaggtgcag caaatttctt tggaattaca aaagacggaa
    21421 aattcatcac tccgcaatcg ccctctatct taccaagtat cacgaaatat tccttgttgc
    21481 agttagctga agaacgacta ggactgcaag caattgaagg agatattttc attgatgatc
    21541 tagatcaatt tgcagaagct ggtgcatgtg gtacagcggc tgttatttct ccaatcggtg
    21601 ggatttatca caataacaat cttcatgtat tttatagtga aacggaagta ggaccagtaa
    21661 caaaacggtt gtatgatgaa ttgacaggta ttcaatttgg agacatagaa gcaccagaag
    21721 gctggattga agtcgtggaa taatttcaaa gtaatg
//
LOCUS       DAYYGO010000044        21302 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000044 DAYYGO010000000
VERSION     DAYYGO010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.045"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ACHA5T_001346"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ACHA5T_001346"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFM8886929.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ACHA5T_001347"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ACHA5T_001347"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFM8886930.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVDCREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ACHA5T_001348"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ACHA5T_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFM8886931.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ACHA5T_001349"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ACHA5T_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFM8886932.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ACHA5T_001350"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ACHA5T_001350"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFM8886933.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ACHA5T_001351"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ACHA5T_001351"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFM8886934.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="ACHA5T_001352"
     CDS             complement(8053..8523)
                     /locus_tag="ACHA5T_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFM8886935.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="ACHA5T_001353"
     CDS             complement(8569..8994)
                     /locus_tag="ACHA5T_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFM8886936.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="ACHA5T_001354"
     CDS             complement(9061..9330)
                     /locus_tag="ACHA5T_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFM8886937.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="ACHA5T_001355"
     CDS             complement(9341..10936)
                     /locus_tag="ACHA5T_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM8886938.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="ACHA5T_001356"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="ACHA5T_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFM8886939.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /gene="pth"
                     /locus_tag="ACHA5T_001357"
     CDS             complement(14489..15049)
                     /gene="pth"
                     /locus_tag="ACHA5T_001357"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFM8886940.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="ACHA5T_001358"
     CDS             15402..16376
                     /locus_tag="ACHA5T_001358"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFM8886941.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="ACHA5T_001359"
     CDS             16546..17946
                     /locus_tag="ACHA5T_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFM8886942.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="ACHA5T_001360"
     CDS             complement(18024..18746)
                     /locus_tag="ACHA5T_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8886943.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="ACHA5T_001361"
     CDS             complement(18825..19331)
                     /locus_tag="ACHA5T_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM8886944.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="ACHA5T_001362"
     CDS             19454..21253
                     /locus_tag="ACHA5T_001362"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFM8886945.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLFQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
BASE COUNT         5925 a         4668 c         3840 g         6869 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gttttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaatc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc tcctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatgggat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttatact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggtcgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttttca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ct
//
LOCUS       DAYYGO010000045        21047 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000045 DAYYGO010000000
VERSION     DAYYGO010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21047
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.046"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            450..1829
                     /gene="brnQ"
                     /locus_tag="ACHA5T_001363"
     CDS             450..1829
                     /gene="brnQ"
                     /locus_tag="ACHA5T_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFM8886946.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            complement(1985..2431)
                     /locus_tag="ACHA5T_001364"
     CDS             complement(1985..2431)
                     /locus_tag="ACHA5T_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFM8886947.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(2458..2634)
                     /gene="rpsU"
                     /locus_tag="ACHA5T_001365"
     CDS             complement(2458..2634)
                     /gene="rpsU"
                     /locus_tag="ACHA5T_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFM8886948.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(2796..3224)
                     /locus_tag="ACHA5T_001366"
     CDS             complement(2796..3224)
                     /locus_tag="ACHA5T_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM8886949.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            3316..4176
                     /locus_tag="ACHA5T_001367"
     CDS             3316..4176
                     /locus_tag="ACHA5T_001367"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFM8886950.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPSVEFNLFRRT
                     FVTDEKTLMQALQDALEEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            complement(4260..4610)
                     /locus_tag="ACHA5T_001368"
     CDS             complement(4260..4610)
                     /locus_tag="ACHA5T_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFM8886951.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAIIQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(4626..6353)
                     /locus_tag="ACHA5T_001369"
     CDS             complement(4626..6353)
                     /locus_tag="ACHA5T_001369"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFM8886952.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     TIIQKMTEE"
     gene            complement(6566..7492)
                     /gene="trxB"
                     /locus_tag="ACHA5T_001370"
     CDS             complement(6566..7492)
                     /gene="trxB"
                     /locus_tag="ACHA5T_001370"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFM8886953.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(7637..9070)
                     /locus_tag="ACHA5T_001371"
     CDS             complement(7637..9070)
                     /locus_tag="ACHA5T_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFM8886954.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(9070..10473)
                     /locus_tag="ACHA5T_001372"
     CDS             complement(9070..10473)
                     /locus_tag="ACHA5T_001372"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFM8886955.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMNELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            10703..11605
                     /locus_tag="ACHA5T_001373"
     CDS             10703..11605
                     /locus_tag="ACHA5T_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8886956.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            complement(11646..13532)
                     /locus_tag="ACHA5T_001374"
     CDS             complement(11646..13532)
                     /locus_tag="ACHA5T_001374"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM8886957.1"
                     /translation="MGKYQELASKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGVVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTALLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPAFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNPGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEAGETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVTNYTDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(13629..14480)
                     /gene="licT"
                     /locus_tag="ACHA5T_001375"
     CDS             complement(13629..14480)
                     /gene="licT"
                     /locus_tag="ACHA5T_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFM8886958.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELYQVIQKKYKDTFGCVEKIADFLAEIFNYQMSTDEKMYLTIHIARL
                     VHENRCK"
     gene            complement(14938..16722)
                     /locus_tag="ACHA5T_001376"
     CDS             complement(14938..16722)
                     /locus_tag="ACHA5T_001376"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8886959.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPTITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLTKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            complement(16722..18473)
                     /locus_tag="ACHA5T_001377"
     CDS             complement(16722..18473)
                     /locus_tag="ACHA5T_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFM8886960.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLAPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAALLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGCSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQKLEAKIEGGEA"
     gene            complement(18615..18839)
                     /locus_tag="ACHA5T_001378"
     CDS             complement(18615..18839)
                     /locus_tag="ACHA5T_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFM8886961.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            19090..20676
                     /locus_tag="ACHA5T_001379"
     CDS             19090..20676
                     /locus_tag="ACHA5T_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFM8886962.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETIEEIE"
BASE COUNT         5973 a         4511 c         3743 g         6820 t
ORIGIN      
        1 aaaagaaaac aaaatagtta aaatattctt gcatttcaaa aaaaacaggt gtattattac
       61 gtcaacaaat aaacatacag caaagcgtcg aggaaaacag taggctattc aacgatttat
      121 tcccagtgag tcatgtaagt gagaaatgac aataaatcga atagacgaac atcatttccg
      181 agtcaatgtc gaaaacgtag atcattgtgg ctgcctccat tatcaggcta gcggataact
      241 ttatcaaaaa gcgtccgtga atgaagaagc ttttttaagc gaaaaaaggt ggtaccgttc
      301 tagaatctag agcccttttc ccaagtggaa aagggctttt ttttatattt taaggtggtg
      361 gttttcatgg aatctgatat tagtggcatt actaattgct gcaacgttta tatcgtcaat
      421 caaaatgata attattggag gaaaaattta tggaagaaac aaaaacttat tcatggaaac
      481 aactattgat ggtcagttca cttatcttcg ggatgttttt cgggtcaggt aatctaatct
      541 ttcctgtcca cttaggtcaa atggctggcg caaactgggt cagtgctggc gtaggatttg
      601 ccatctcagg cgctcttttc ccattactag ctatcttagc tgtagtagtg acaaaaagtg
      661 atggtctgta tgacttagct aaacctgtcg gaaaatggta tgctgccttg ttcttaatcc
      721 ttgttcattt gacgatcgga ccatttttcg gtacaccacg gacagctgct actgcttttg
      781 aaatggctgc taaaccattt ttgcctgaaa aatataattc attaggtatg tttttattct
      841 ctgctctttt ctttttactt gcttatttcc ttactgtcca tcccacacgg ttggtcaaat
      901 acgtcggaaa gtatctaaac tccttgtttc ttgcattatt agcaatcatt tttgtcgttt
      961 ctttattcca cccaatggga aatctaaatc aatctgctgc agatacttac caaacaaacg
     1021 ctttaacaaa tggtgtccta gaaggttata acacggttga tgcagtagcc ttgcttgcct
     1081 taagtgtcac ttttgtccat gcagtccgtg accttggttt caaagataaa aaagtcacag
     1141 aattaacagc aaaagctggt agcctgagcg ttgcgctaga agtcatgatc tattttggtc
     1201 ttgttcttct cggtgcgatg agtttgaata agatgtcttt atcagaaaat ggcggaactg
     1261 cactttctca gatcactaat ttttatttag gtaacttcgg cgctagcttc ttaggagtga
     1321 tggtcacatt aggtgtcttc actaccgcta tgggactcgt tgtttctttt gcacaagatt
     1381 tccataaact gttccctaaa gtcagttata tgacatggct gcggttaacg actttcgtct
     1441 cttttgtcgt tgcaaatgct ggtttggaca atatcatcca atggtcttta ccagtattga
     1501 tgcttttgta tccattgtcg cttgcgttga ttttgctttc cttaacagca aaatttttcc
     1561 aaaagacacc atttgtctat caagtaacga tgttgtttgc tgctgttccc gccgtattgg
     1621 atatgctggc aaattcgcct gctttagtga gtcaacaaag agttgtagca agcatgctcg
     1681 aattttatca tcatcacgtt ccttttgctg cattgggact tggctggatg gtcccaacgt
     1741 tacttgggta tgctggcagc ttactcttct attacgccta tcgcctatca gggtataaac
     1801 aagaagcaaa tgaactacca gaagaataac cagatcaaac acaatttaac ataagaaata
     1861 ttaaacagga aaaaaacagc tagatgaaag aatcctattc tttcgtctag ctatttttta
     1921 gatctctttt ttacgagcac aaaaaaactg cggacaatga ttgtccgcag tttaaatgat
     1981 aaaattaaga ttgtaatagt tctttcacaa tggcatttac ttggtttcca tctgcttttc
     2041 ctttgacttt aggcatgatc gcacccatga ctttgccaaa ttctttcatt gatgaagcac
     2101 cagtttgcgt gattgcttct tgaacaagtt gtcgaatttc ttcgtcggaa agttgttccg
     2161 gcatgtattt ttcgactatc acaatttcac tttttacttt ttcagctaaa tcgtcacgtc
     2221 ctgcttcttc aaactcatgc aaagaatcac gtcgctgttt catttcgcga gacaacaccg
     2281 ttaattcttc ttcttctgtc aagtcgtgac cttctttaat ctgctcgttt tgtatagatg
     2341 acttgatcat tcggatgact tgtaaagatt ccttatcttt cgctttcatc gctgttttca
     2401 tgtcatcgtt taatctactt agtagtgaca cactaccaac tccaattcat cacagaacta
     2461 gaattttttg cgttttctag ctgcttcaga ttttttcttg cgtcttacac ttggtttttc
     2521 gtagaattca cgtttacgag attcttgtaa agtacccgct tttgacacgg aacgtttgaa
     2581 gcgacgaaga gcatcgtcaa gtgattcgtt tttgcgaacg actgtttttg acatgttaat
     2641 tccctccctc cgagcttaaa aagtaaccgt aattgttatt gaaatatatc tcaaaaaaca
     2701 agactgtcct gtaaatatta tatctaatgc tgttttgacc gtcaaggaaa tcttgctttt
     2761 ttttaaaatt atcaaatgtt attttattta aatccttatt gacaattttc acatcttccg
     2821 aaaatctcga atctatgccc ttcgatcgta catccaccga gctgttcttc gaaataattc
     2881 attggacaca tatggatttc tttcgttgtt ccgcacaccg tacagataaa gtgatggtga
     2941 tggccgattt ccccttgaca gcaatgaaaa cgaaacttca tctctccatt taaatcggtt
     3001 tcttccagta aatcgatttc tgcaaaatca tggagatttc gataaatcgt atcgtaactt
     3061 actcctttga attcctgatt catatagtca aaaacttctt tagcggatac gtagcgattg
     3121 cgcttcacta aataagttaa caatgcttct ctttttttag tatatttaaa accgttattt
     3181 ttcaatgttt ctagtgattg ttcgatcaac gtctgttttt tcatgactta ttcctccaaa
     3241 tcaaaatgat tacgaagtac acattctaga gtatggtata ataaacataa aaaaaatgca
     3301 agtgaggttt tatccatgtc ttccgattct acagaaatcg ttactttttt tgtcatctcc
     3361 gactctgctg gagaaactgc gacaaaacta gcgcaagcca ctatggccca atatcctagc
     3421 gttgagttca atttattccg cagaacattt gtgacagatg agaaaacatt gatgcaggca
     3481 ctgcaagatg cactagaaga aaaagctctt gtcctgcaca cattgatcaa tcaagaattg
     3541 atcgagatga cgcgttcttt ttgtgaagaa catcaattat ttagttttga tgtgatgact
     3601 ccgccgatat cagaaatcga gcgcttaact ggtatcaagc ccattcggca acctggcgcc
     3661 cttcatttat tgaatgagaa ctatttcaaa cggatcaaag caatggaatt tgctgtaaaa
     3721 tacgatgacg ggaaagatcc tcgtgggttt ctagaagcag atgtcgtttt gctaggcgta
     3781 tcccgaactt ccaaaacgcc tttgagcttg tttttagcta acaaaaattt aaaagttgcc
     3841 aatctacctt tgatcccaca agcacatatt cccaaacagc tttgggagat cgatcctaaa
     3901 aaaatcgtgg gactgacgaa taacccagat attttaaaca atatccgtaa agaacggatg
     3961 cgctcttacg gattaaatcc tgatactgct tattccgata tcgaaaaaat acgagctgaa
     4021 ctcgattttg cgaatgatct ttatgaaaaa ttgggctgcg tagtaatcaa tgtcgcttct
     4081 ttatctattg aagaaacagc ttcgcttatt ttgaatgctt tagacctaga agaccatagt
     4141 tattatggaa ctgaaaccac cgaagaaaca gactaacaat ttttctttaa aaaaactgga
     4201 ctgtgtcagc gaagaagatt cgttgacaca gtccagtttg tctctatttg cacctatcgt
     4261 tattgatgtt ccacatgttt gagtgtctgg ttcaaaatat ccagtacgtg agaatcatca
     4321 agactataca aaatcgcttt tccctcacga tgggacttga cgacatggtt ttctcttaat
     4381 aaacgcagtt gatgcgatac agcagattgt tccatttcca ctgcttggat gattgctgtc
     4441 acattttgtt ccccgtcttt cagatacagc agaattttga gacgagtggg gtcactcaac
     4501 attttgaata gttggctgac tttctctact tttgtttttt gcgtgtctgg caatccattc
     4561 acctccaact tgaaaaaaga ctgacttcta aagtttacca ctttttccat cagtctcaag
     4621 tttttttatt cttctgtcat cttttggata atcgtttcat aagcggctac tttttcagct
     4681 gcgtcttctg aagagtttcc ttttacaccg atataaaatt tgattttcgg ctcagttcct
     4741 gaaggacgga tagcgatcca gccattttct tccagcgtat acttcaatac gtcagatggc
     4801 ggtgtcgtta gtttttctac gtttccttca cggtctgttc tcgtcaactc tttgaagtct
     4861 tctgtttgga tgacttttgc tccggcaaat tctgttggtg cttcttcacg gaatttttcc
     4921 atcaaagcag agattttcgc agaaccttct tgtccgctca ttgtcacaga aatcgttttt
     4981 tctgcgtagt agccataggt ttcaaaaatc gtttgcaagc catcatacaa cgttttgcct
     5041 tgttttttat agtaagctgc tacttctgct aataagagca atgcttggat cgcgtctttg
     5101 tcgcgaacga aaggtttaac taaataaccg taactttctt cgaatccgaa cataaaagta
     5161 tgggaatgat cttcttcaaa ttgttgaatt ttttcagcaa tgaatttgaa tcctgtcaat
     5221 acattgaaca tggtagcccc atagtgttcc gcaattgctg taggtaattc gctagaaacg
     5281 atcgatttca ataccgctgc gttttcaggt aaagtgcctc gcgattggtg tgcttccaaa
     5341 atatattgag caaacagtgc tccgatttgg tttccggtca atacttcata ggagccattt
     5401 ggcaaacgaa cggctgcacc taaacggtct gcatcaggat ctgttgcaat cagcaaatca
     5461 gcgccttctt tctcgccaag tcgtatcgca tactcaaaag cagagtgttc ttctggattt
     5521 ggtgatttga ctgtagtgaa atctgggtct gcgacagctt gttctggaac tagtacgaat
     5581 tgttcgaatc cagcttgttt caacgcacgt tcgcccaaca tttttcctgt tccgtgaagt
     5641 ggtgtgtaca caagtttcaa atctttcccc attgtttcca ccaattcatg atcaatcgtt
     5701 actgctttga tttcttctag ataagcggca tccacttctt ctccgatgat cgtgatcaat
     5761 ccgctatgtt cagcttcttc gtcagataat acttcaactt ttaacggatt gctgacttca
     5821 cggacatatt tcgtcaatgc atctgcgtct gcaggaggca tttgtccccc atcttctccg
     5881 tagactttat atccgttgta agctgctgga ttgtgggatg cggttaccat gattcccgca
     5941 aacgtcttca aatgacggac tgcaaaagaa agctctggtg ttggacgtag actttcgaat
     6001 acataagatg ggatattgtg atgtgctaat gttttggctg cttccatggc aaattctggt
     6061 gattgatggc gtgagtcata agcgatagcg acacctcggc gttttgtttc ttcgccttgt
     6121 gtatccatga aacgtgcaag tccttctgtt gcttgtctta ccgtatagat gttcattcga
     6181 ttgatcccag gccctaagat tccccgcatg cctgcagtac cgaattccaa cggtgcataa
     6241 aaagcatctt caagtttttc aggatcttca cccagatcat ttaattgttt ttttaagttt
     6301 tcttctaaat tttcttcatg tacccattgt tcatacactt gttcccaaga catgaaaata
     6361 acacctcttt ctgaaatatg gttcttaata agtatagcat tcacttatta gatatgaaag
     6421 tatgaggacc aaaaaacgct taaatcaaga aaggattctt ttatcaagca cggttgcaag
     6481 tattcgcata aatcggattc agtagatttc acgcgaaaaa aaagagtaaa agaacaatta
     6541 aaccgttctt ccactctttt taaacttatg cagttgtttc ttctaaagat tccaagtatt
     6601 tatacacttg ttgtccagcg atcccaccat caccaacagc agtagtgatt tgtcgcaaat
     6661 ctttttcacg aacatctccg atggcaaaaa cgcctggtat attcgtcttc atttcgttgt
     6721 ctgttggaat ccagccagct tcgttcgtca accctgcttc tctaaatggt gcagttaatg
     6781 gatccagtcc aacatagatg aatactccgt ctgctgccaa atcaaactca ttgccggttt
     6841 tgacattacg tgctttcaca cctgtcacta ccatttcatt cccaacgatt tcgtcaacga
     6901 ctgtatccca cataaaggaa attttttcat tcgcaaatgc acggtcttgg atgatctttt
     6961 gtgcacgtaa ttcatctcta cggtggacga tgactacttc agaagcaaat tgtgtcaaat
     7021 agatggcttc ttctactgct gagtctcctc ctccgatcac tactagacgt ttgttacgga
     7081 aaaacgcacc atcacaaact gcacagtaag aaacgccacg tccagcaaat tcttcctcgc
     7141 caggtacgcc taatttacgg tggatacagc ctgttgcgat aatgactgtt tttccttcgt
     7201 aagttttatc ttcacaaata acttctttgt ggctgccgtg atccttgata tcttgcacga
     7261 ttccataagc attttctgtc ccaaatcttg aaacaccttc atacatctta taagctagat
     7321 caggacccat tattgaatcg aaaccaggat agttttcgat ttctgccgta ttgttcattt
     7381 ggccgccagg ggcgccttgt tcgatcatca atacggataa gtttgaacgt gaagcatata
     7441 aagcagcagt catcccagca ggacctgcgc caataacgat tacatcatac atatgtagat
     7501 tctcctttac ttaatcaagt aagcatactt tacagccaat gcgaaaaaaa gtctattgtt
     7561 ctgcttgcgt ttctcgaata gctttattat actcgaaaaa tccgctgaaa aacaatagac
     7621 ttttataaat cattgatcaa tcattcgaaa gattttctcc atttgaatca attacttttt
     7681 tataccagtt gaatgatttc ttcttcgaac gggctaatgt gccatttcct tcgttgtcac
     7741 gatcgacata aatgaatccg taccttttct tcatctcacc tgtaccagct gatactaaat
     7801 caatacatcc ccaagtcgta tagcctaata gatcgactcc atctaattct actgcatctt
     7861 tcattgcctg gatatgtgct gccagatagt cgattcgata gtcatcttcc acataaccat
     7921 tctcatcggg cgtatcgact gcacctaagc cgttttctac aataaatagt ggtttttgat
     7981 aacgatcata aatatcattc atcgttattc tcaagcctaa tggatcaatt tgccaacccc
     8041 attcacttgc ttccagatat ggatttttga cagaagcaaa gatatttcct gcagtctgtt
     8101 ccaagagttc gttgtcagta gtcgaaactc gagaagaata ataagaaaag gagataaaat
     8161 caacagtatg ttctttcagc aatgcggcat ccccaggttc cattttgatc tgaatgccat
     8221 ttcgttccat ttctttcaat gcataagcag gatattctcc tcgtgactgg acatcaatga
     8281 agaaataatt ttctcgatct gattttctag aagcccaaac atcttcagga ttgcaagtgt
     8341 atggataata tgctcctgct gccagcatac agcctacttt gttctccgga tctacttcat
     8401 gagcaatctt cgttgcgatg gcacttgcaa ccaactcatg atgtgccgct tgatatttga
     8461 tttgttcttc attttctcct tcttcaaaat acagccctgc gcccatgaat ggtgcatgaa
     8521 ggatcatatt gatctcattg aatgtcaacc aatatttgac aagtccttta tagcgattga
     8581 agatgactcg gcacagattt tcataaaaac caacgagttc tctggaacgc cacgcaccat
     8641 attctttgat caaatgcatc ggacaatcaa aatgtgtaat cgtcacaagc ggctcgatcc
     8701 cgtacttttg acattcttta aagacatcct cataaaattt caaaccagat tcatttggct
     8761 ctttttcatc gcccattgga aaaatacgac tccaagcaat ggacatccga taagtcttga
     8821 accccatttc tgcaaataaa gcaatatctt ccttataatg atgatacata tcgatcgcaa
     8881 tttttgctgg ataaaaatga ttttcatcaa attcaaacat tttctttttt ccagtaatga
     8941 caggaaaacg gtcttctccg attggcacaa catctacgtt cgctaaccca cgtccgcctt
     9001 catcataacc gccctcgcat tgattggctg ctgtagctcc gccccataaa aaatcttttc
     9061 tgaatcccat catacttcct ccattacatg tgtattgctt gataattctc ttgccagttc
     9121 atcttggact tcgattcctt ctgcttttaa ccggctgatt gtttccttga attgcggtaa
     9181 atactgctga tgtgcaataa acaactcatt catgatccgt tttgctgtct ttcctgatcg
     9241 tataagcgga ttgatcgtaa acgcttgtaa agctgttcca taatcaccag tcacagctgc
     9301 ttcgatcgtc agcaattcca tattcttcat tacttgcaac cagcctcttt cagcagcagg
     9361 aagggaacca aacgctacat ttcgtgcgcc ttgtgcgcca acataggcag tgacttctac
     9421 tacacaatct gccggcagat caggaacagc tccatcattc tttgtagata ccacgatctg
     9481 tgtttcttta tttccataga tcgaagcaat cgtttcacag gcagcatctg aataataagc
     9541 accacctcgt ttttgcaatt gttcaggttt atgatccaat tctggatctt tgtataattc
     9601 aaacagctct gcttctgttt ctttcacttg ctgcgctctt gtaccaatcg tttgatattc
     9661 ttctaaagca tgttccagca tttcttcttc acggtagtaa tagcggtggt agccacaagg
     9721 gatcatgttc atttgtttta attgttcttt gaaaaacggg atatcaaaga tatttttcgg
     9781 caatccattg tcttcatcgt ataaacgatc aatcaattct gccgtgcggt catttccttt
     9841 attatctgct actttgtgcc aatggaagtg atttagaccg gcaaacttgt agatcagctc
     9901 ttcttttttc ttgccaagca ttgttggttc tgtcatcatc gccattactg gcacattgca
     9961 taatcctatc actttatccc atttgccata tctgataact gcttctgtca ccatcccgct
    10021 tgggttcgcg aaattgatca gccaagcatt tggacataaa cgcttcatgt cttcaacgat
    10081 tgccaaaacc actggaacgg tacgaaaagc tttgaatatc ccgccagcac catttgtttc
    10141 ttgcccaatc atcccataat aactagggat ccgctcgtct ttgattcgtg catctaattg
    10201 accgacacga aactgagtcg taacaaaatc cgcatctttc aatgcttctt cccgatccag
    10261 agttgcatag attttgactt catacggaga agcatcccac atacgctgag acatctgact
    10321 gacgatctca agcttttctt ttccttcttc tacatctacg agccagattt ctcgtatcgg
    10381 taattcctca tatcttttga taaatccttc catcaactcg ggggtgtaac tgctgcctcc
    10441 accaatcgta gcgattttga ttccttttgc catgttctca ctcctttact ttttacactt
    10501 ttattatctt ttttgtaaac aaaacaaaca atagaaattc gtttctttcc agtaacattt
    10561 acaaaagtta gtgttttcta ctggattttt gtaaatatct tgtcaaaaaa tgtctttatt
    10621 aagctatata acataaatcc gtttacaaat cattttcaaa aaagttataa tttacgtaaa
    10681 aaaacaagaa gaaggtatct acatgttatt acgtgaaaaa atcaaagaaa tctccttttc
    10741 tccttcagaa aatgaagtga ttcagtttct cttaagcgat tctccagcac ttggcgaaat
    10801 gaccatccaa gagattgctg agcgatgtta tgtccacccg tccacactca tccgtgtagc
    10861 aaaaaaaatg ggttttgatg gctgggtcag ttttcgtaat gcttttttag ctgaaacagc
    10921 ctatcttgaa gggaattttc aagacgtaga tgcgaatctt cctttttcca ccacagacgg
    10981 tattatgacg atcgcaaata aaatggcttc tttagaacag atgacgattc aagatacact
    11041 ttctctcatt catcatgacc aactgcaaaa agccaaacaa ctaatgcttc aagcaaaaaa
    11101 aatcgtgatt ttcgccagta actcgaatac tttgatttct caagatttca tgctgaaaat
    11161 gaagcgcatc aaaatggatg tcacgattgt caccactttt ggtgaatcgt tctatgaagc
    11221 gtacaattgc acgagtgata tgtgcgggat tctgatttct tatacgggtg aaaacaatgt
    11281 gatccttcgt accgcagaaa tattgaaaca aaatcatgtt ccaattttag ctttgaccag
    11341 tatcggtgat tcaacgctat cttcaatgag tacagccgct ttgcgactta cgacacgcga
    11401 acgactgtat tcgaaaattg gtaattttac gatcaataca tcgatttgtt atcttctaga
    11461 cgtgttatat agttgtgttt ttgcagaaaa ttatcaacag aatttagtcc atctaatttc
    11521 tgtaggacaa aaatttgata aacgccctat cagctcagcc atcatgaaag agcctttcaa
    11581 ttttgaaaat aaagaagtgg actaaaaaga ataattaagc ctagtccatt ctttcaccta
    11641 accgttcagg tcagtgcggt gataagtgca tcgtctggcc cgaccgtctc cccttgattt
    11701 tccaaaatat ccaaataatc cgtgtaattg gtgacgatca caggtgtttc taaactgtat
    11761 ccttcctctt cgattttctt tttgtcgaag cggagcaaca gatcgccttg tttcactctt
    11821 tctccttgct gcaccaagct ttcaaaatat ttcccttcca actgaacagt atccaatccg
    11881 acatgaatca gcaattccag acctgcttca gaaaccattc cgattgcatg tttcgtcggg
    11941 aacagggtca tgactgttcc gtcaaaaggg gcacgtactt ctcctttttc agggtagatc
    12001 aagattcctc gacctagtgt tccttgtgca aaagctgcat cctttgctgc attcagtgga
    12061 gcgatttgtc cttgtatcgg tgtttttaca atttctttac ctgatctttt ctgtctatca
    12121 tctgtttcac ctgcttcctc cttccaaaag aaaaaagtca gcgcaaagga taccaccatc
    12181 gcaatggcga ttccaatcaa tacattaatc acatccgtta aatcaccatc tggtctaata
    12241 tagttgggaa ttgtaaagat gcctaatcca cctggattcc aagaagttaa attcattaat
    12301 ccactatacg cgccccctaa cgcaccaccg acacaggtaa aaataaatat ttttttacgt
    12361 ggcaatgtaa acccataaat agctggctct gtaatcccca caataccaga aacaatggct
    12421 ggtattgcga ttgctttgtc ttttgggttt ctaagcttaa agtagagtgc agtaagtgct
    12481 gcagtttgtc caaaagacac tactgcaaca ggtgtcatga tattttgcca accattcaca
    12541 gaaaattgca tcaatcccat tggtacgatt gcccaatgga gaccaaacat cactagaatt
    12601 tgccagaaga agcctacgat tgctccataa ataactggcg aaagtgctaa aagtgtttca
    12661 aatccagcca ttaaaatatc agttgcgaat gtgaatgctg gtccaacgag taaaaaacct
    12721 aagggcattg taataagaac ggtgaagaat ggaacgaaga aattcgcgat catctcaggt
    12781 actaactttt taattggttt ctgaatttgg ctagctaaca acacaataaa gataatggga
    12841 atgacagaag aagcgtagtt acgcgcaatc cagggaatgc cgaatactgt ttgataaata
    12901 ggtgcttcaa aaatagtgcc agaaaatatg gtagtcagtg gtgcttctgc tgcaccactc
    12961 aaagcttcca gcgacaagct aggattcatt aatgctgccc caagcaacat ccctacgaat
    13021 tcatttactt taaatttacg ggcagctgtt aaaccgataa taacaggcat aaattggaat
    13081 acagaatcac cgatattatc aaacataaca aatgtgccag aaccgcggtc aaatgcgcct
    13141 aagaagacaa gtaatgctgt cagtccacga atcatcccac cagcagctag aatagcaagg
    13201 aatggctgga aacagcccga tattccatca ataagcatat caaacggtct cattttttct
    13261 gttgaaacag cctcatcaaa agtaaggttt cctgctttta taacctcttc aaacacgact
    13321 ggcacatgat tcccgatgac aacttggtac tgtcctcccg ctcgcattac cgtcacaaca
    13381 ccgtccatat ttttcaagat ctcatcattt gctttttctt cgttttttaa tttgaaacga
    13441 agccttgtga tacaatgtgt caaaccattg atattttcct tcccgccgac attttctaca
    13501 attttgctcg ctaattcttg atattttccc atttctattc cctccaattt attagtggaa
    13561 ggtttagcca actgaatgtc actaccctta tttcatgcgt tcttgttaat ttatcctcct
    13621 ttttttattt atttacaacg attctcatgc actaaacgcg caatatggat cgttaaatac
    13681 attttttcgt ccgtggacat ctggtagttg aatatctcgg ctaaaaagtc tgctattttc
    13741 tcaacgcaac caaacgtatc cttatatttt ttttgaataa cctggtacaa ttcctcagaa
    13801 acttctccca cttctgattt ttctccatct aggacgcgtg tcagaaaaaa acgtaggtgt
    13861 gtgacgaatc gaaaaaaatg gactgaatct tcatcaaatg taatcttata gtaatagcgg
    13921 acgatattta gaatatcctg tattgttgta gtaaacaggt aggtattgtc ttgttggctt
    13981 tgaatgcctt gtgcattgac taaatgcaat gcgataaatc cggcttcatc ttctgataac
    14041 ttgatgtggt atttatccga gattttcttc actgtttttt tcccgatggc atactcttta
    14101 ggaaatagtt gtttgatatc atacagcaat acatttggta caaatattcc cattttgtga
    14161 cgttcgatag ccgtatacaa atgatccgtc aatgcaatat acaccgaatc attcaactgg
    14221 acttgtagag cctcttttgt atattcaatg atttcacaag aaatttctaa gtactcaaga
    14281 ggaatcgctt gtgccaattc ttggaactgt tgtgaaagtc cgtcatttgt caactgaaat
    14341 tttttgtcga tgcatgagtc aaaaatcggg tcacccactt tctttttgaa tgccaaacct
    14401 tttcccatca cgatcatttc ctgatttctt tcatccctgg ttattacgac gttgttattc
    14461 agtactttct caatcagcat gttttactcc tttccatgat atatagggct gtatccaaaa
    14521 caaaaagaaa aacccaaaaa gccgtccatg gaaccatggg agactcatca ggtttagctt
    14581 gcatcaggca atcactatcc aagttgtatc gttttcttta tattatcgaa agcaagcact
    14641 tttgtcaacg ctttcttagc ggctattggg cacttctgac tagctgtcgt gatttttttt
    14701 aaatctggta attacgacac taacgctcac agatgaagaa tctgggaagc tctgttttat
    14761 tttcactcta gcgcttaggt caaaatgcta ctatgctact gtcccaactt ctaatatacg
    14821 gtgttccagt accagcttat cggtgataag ccaaatttat gcaaaaatct ggaaagcctt
    14881 ttttacaatt tttttcttat cacctaaagc tgaacggagc cctgtcacaa ccacttttta
    14941 cacaccactg ctttgcagtt tgtacatatc tgcatacaaa ccattttgcg ctagaagtgt
    15001 ttcatgattc ccacgttcta ctatatgacc tttgtctaaa actaaaatca attctgcatc
    15061 ccgaatcgtg gacagccgat gggcaatcgc aatcgttgtc cgtccttgtc gcatttttgt
    15121 caatccttct tggatcaatc cttctgtttc agtatcgata tttgctgtcg cttcatccaa
    15181 tactagtatt ttaggatctg tcacgattgt tcgcgcaaaa gtgatcagct gtcgttgtcc
    15241 gctagaatag ctggcaccgt gttcgatcac ttttgagtga tatccttttg gtaattcatc
    15301 gatgaacttg tttgcttgaa caaactcagc tgcatggcga atctgttcgt ctgtgatagt
    15361 tggattcatc atccggatat ttcctgcgat atctccataa aacaagaagg catcttgcaa
    15421 gaccaatccc attttcttac gtaactcttc cattggatat tctcgaatgt catgatcatc
    15481 aatcaagatc tggccttcat aaaattcgta aaaccgcatc aatacgttga tgatcgagct
    15541 ttttccgctt ccggtgtgcc ccactaatgc aacggtctct cccggattag cgacaaaaga
    15601 gatattgttc aatacattgt gctctccatc ataagaaaag cttacattgc gaaactcgat
    15661 cttacctgaa gtaatcgctt gatctgctcc aggattttgt tgcggtgcat attcttctgt
    15721 atccatgatt ttcaaaatac gactaccggc aaccattcca tctgtaaagg tgctcaaaaa
    15781 gtccatcatt tgagacattg gattaaaaaa tccctggaca taggtcgtaa atgcatagat
    15841 cagtccaact tctacgggag aatgcagcgc atcgatccca aacaaagtca acgccaacgc
    15901 aatcgccaat gcatatagaa aattgatgat cggacttaac agaatcgaat ttgttttgat
    15961 catcgcatag cgcgtgtcta aatactcgtt gttcacttgt tcaaattctt tagctaaacg
    16021 agcttcttgg cgaaactgct ggataatacg catgccggaa atcgattcat tcaatttcgt
    16081 gttcagctga cttaattttt cccgcatatt ccggtaaata cgagaactga atttctggta
    16141 ataccagatg acgattccta aaatcggcaa gaacaccaaa gtccacaagg caatttctcc
    16201 attgatttga aacatggcaa tgaaggaaga aacgactgca aagatcccag tcaataccat
    16261 caaaaagaca taccaaaatt caaatagcgt ttctgtgtca tttgtcactc gagaaacagt
    16321 cgatcctgcc ggtgtctgat caaaataacg catacctagt gtatgcagct tttcaaataa
    16381 tcttacccga acatactgat aggtcttcaa tgaagccatg gaatacaaat accattggaa
    16441 aaaccaaatg atacttttga ctatgactcc aaacaagtac ataccagcaa aaaacaaaat
    16501 aacttgtgtc gtagccgact gtttagccag atgatcatcc ataaaagttt ggatgatcct
    16561 tggcaatagg acattgatca cagacagggc aaacgcaaat aagatagcaa cgatgaacgt
    16621 tcttctaaaa ggtttggcga acttcatcag tcgtttgatg atcttcttct gttctttaag
    16681 tggaatcgat tttgtccatt ccgattgata attttcatcc atcaggcctc gcctccttct
    16741 atcttcgctt ctaatttctg tttctcccac attcgttcat accagccgcc taatgcaagc
    16801 agctcatgat gggttcctcg ttcaatgatt ttcccttgat cgacaacaat gatttctttt
    16861 gcatgcatca cgctgcttaa acggtgagct gcaatgatgg ttgttttctc ttttcttatt
    16921 tcttttaaat tatgcaagat agcttcttct gttttggcat ctactgcaga aagtgcatca
    16981 tctaagatca gcaattcagg atttttgatc aatgctcgag caatcgaaat cctttgtttc
    17041 tgtccgccag ataaagagac tccacgttct ccaactaagg tatcataacc ttttggtaat
    17101 tccttgattt cattgttgat aaatgctaaa gaagccgcat gctctacttc ttcttctgaa
    17161 agttttggat tagaaaaacg gatattatct cgaatggtca tcgaaaacaa gaagtgatct
    17221 tgaggcacat agccgattga tcctaataaa gcattcaatg aatattcttt gatcgaacat
    17281 ccgccaaatt gaatttttcc ttgataatca tcgtattcac gcatcagtaa tttgatgatt
    17341 gtcgtttttc ccgcaccagt tttcccaaca atacctaatg tttctccttc ttggatcgca
    17401 aatttgattt gttctaatgc tggtgattct tctttcggat agttaaaaga ggaaatagct
    17461 acattcagct cgccttttgc cggagtctgc actgcaccca gttgttccac gatatgcgtc
    17521 ttttcatgca aaagttcatt gacgcgatca taagaagcat ttcctctttc caatacgttg
    17581 aacaatcgac caatcgcaaa catcggccaa acaagcatac cgatatagct aataaatgag
    17641 accatctgcc cgattgagat cgtgccttcc aagacatact ttccacctaa aataattgtt
    17701 gcaacataag acagaccgat aatcaaggta atgaatggat caaataagga gtctaaaaaa
    17761 ttgactcgtt tgtttttaat aattgcatct tcaatctttt cttcaaaatc cgcgagatct
    17821 tctttttctt gtccaaatgt tttaatgact ttgatcccag taatactttc ttgtgtctta
    17881 tcattgatac tagaaaaagc ttcttgcgaa tcacgaaaag catcgtggag ttttgacccc
    17941 agcactcgtg aagtaacggc tagtaaaggc agaggaatca gcgcaatcag tgttaagcgc
    18001 caatcaataa acaaaatcat cgcaatgatc gttgttccgc ccgtgatcac tgaatctgca
    18061 aaggtcaaga taccagctcc tgctacattt tggattgcat tcaaatcatt cgttgcatga
    18121 gccatcaaat ctccagtccg atatttttgg taaaagagat tgtccatttc tgtaaaatgt
    18181 ttgaatagct gctgccgaag ttctttctcc aaccgagctg cacttcccca aatattcgtc
    18241 cgccagatat aacgaaaaag atattgtaag agagctgcaa ggatcaataa taagatccac
    18301 caggaaatga tctttattga taaattttta tcggcgatct catcaatgat gatcccaatc
    18361 acttttggag gagctaactg taccaaggcc actgcgaata gggagaaaat cccaattaaa
    18421 taatgtttct tttcctgtcg aaaaaaccaa cctaattttc taaaaatcga cattttactc
    18481 ctcctttgtc tttatcaaga gcataggatt gtctgtgctt gtcttttttg ctgaatagtc
    18541 tgaatagtct aacaaattac gaaataaaaa acggactgaa aaaatcagcc cgcaaaaagt
    18601 ctttgggtca gaacctattt tttcccttgg ttcttcattt gctgcatcat ctggcgaatt
    18661 ttcttctcag atggtttttg ccccattgac atcatcattg atcgcaacat gtcctcatta
    18721 acgggaggat ttttttctaa atagtctttc atatatttac gcgctaggaa aaagccacct
    18781 actgcgccga ttagcaaagc aataatagca ataagtacga caatacctgt tgataccata
    18841 taaatctctc ctttctattt ccgaattgct ctcaatgatt tattttacta aagtttattg
    18901 aaaaaagaaa ggcgtattct ttagtttcat gcaaaaaata gtggtttttt tagagaagaa
    18961 caaaatttct ttcattaacc agcaagacac gccacgaaaa aaaattaggc ataccaaatc
    19021 tttttcttta attttttctt tctgaggtgt agaatgtaat cgtagataaa taggaaaggt
    19081 ggttttttct tgaataatca atccaacaaa caatttcata agcatcgact tattgaacat
    19141 acaaatggtt tatctttagc tgaagtaaat ggaacagtgg atgtacccaa gggaaaaagt
    19201 ttttttaaaa cacttcttgc ttactctgga ccaggtgcat tagtagcagt tggttatatg
    19261 gatccaggta actggtccac ttcgatcact ggtggacaaa atttccaata tttattgatg
    19321 tcagttattt tgatgtctag tctgattgct atgcttttac aatatatggc agcaaaatta
    19381 ggaatcgtga cccaaatgga tttggcccaa gcaatccgag cacggacaag taagacattg
    19441 ggtgtcattt tatggatctt gactgaatta gccattatgg caacagacat cgcagaagtc
    19501 attggtgcag caatcgccct ctacctgctt tttgatattc cattggtaat tgccgtattt
    19561 atcactgtgt tagatgtttt cgtcctttta ttgctgacaa aagtcgggtt tcgtaaaatc
    19621 gaagctttgg ttgtctgctt gattttcgtc attttattcg ttttcgtcta tcaagttggc
    19681 ttgtctaacc ctgattggca cagtttgttt gccggattta tccctaacag tcacacattc
    19741 tcagactcac cggtcatcgg cggcgaaact ccgctgactg gagctttagg gattattggt
    19801 gcaacagtca tgccgcataa cttatatctt cattcagcag tttctcaaac tcggaagatc
    19861 gatcaccaag acgaagaaga tgtagcacaa gcggtacgct tttctacttg ggattctaat
    19921 atccagttgt caatggcttt tctagtcaat gcactgctat tgatcatggg tgtggctgta
    19981 ttcaaaaccg gttctgtcca agatccttcc ttttttggtc tgtatgaagc tttatctgat
    20041 ccatcagcta tgagcaatgg tatcttgatt gctgtagcaa aatctggtat cttgtctact
    20101 ttatttgctg tagcacttct tgcttctggc cagaactcta ctattactgg tacgttgacc
    20161 ggtcaagtca tcatggaagg cttcatccat atgcgtatcc caatttggct aagaagacta
    20221 gtcacacgat tgctttctgt gattcctgta ttgatttgtg ttttgtttac aagtacaaaa
    20281 ggaacgatcg aagaacatac cgctttgaat aacttgatga atgaatccca agtttttctg
    20341 gcttttgcat tgccattctc gatgattcct ttactgatga tgaccaatag ccaagcggag
    20401 atggggagcc gattcaaaaa ttcatttgtt gtaaaactac ttggctggtt ctctgtgatt
    20461 agtctgactt acttgaattt acgaggatta ccaggtcaaa tcgaaagttt cttcggcgag
    20521 caagccacct cagctcaact agcattggct gatcaaatcg cttatgtaat cattgttctt
    20581 gtccttctcc tgttggcatg gacgattggt gatttatata aaggaaacaa aaaatatgaa
    20641 cagcgtttgc aggaaacgat agaagaaatc gagtagaaac tgattctttt acacaaagga
    20701 ggttgtgtca gaagtgggca gcttcaagaa ataagaagga atttaagaaa attgcttttc
    20761 aaatttttgt gaaatttcgg cttatttccg aagaagttac ttctggaaca ccgtgtatca
    20821 gaggttgtga aaaagctgcc ttgcatcaag taataagaac agccaagtaa aaacagcttt
    20881 ttatgttttt aacttggttg gacttattac cgcagatgca agcttttgag caccgtttat
    20941 tcggaaaaag gaactgcgac aagacttttg tcacagttcc ctttcctacg attagtcaag
    21001 taaaaggtgt aagaaaaata gctcataaac gaaagcgaca aaatcaa
//
LOCUS       DAYYGO010000046        20956 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000046 DAYYGO010000000
VERSION     DAYYGO010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.047"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="ACHA5T_001380"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="ACHA5T_001380"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFM8886963.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            2714..2938
                     /locus_tag="ACHA5T_001381"
     CDS             2714..2938
                     /locus_tag="ACHA5T_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886964.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            2951..3454
                     /locus_tag="ACHA5T_001382"
     CDS             2951..3454
                     /locus_tag="ACHA5T_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFM8886965.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKEQIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCNLAEELEGTPIGEAASEIQHAFFNSFEEMVEH
                     VDDIVYYPDCDDMSDVAHYLIVEAGDFGEVPEQLKPYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPE"
     gene            3581..4618
                     /locus_tag="ACHA5T_001383"
     CDS             3581..4618
                     /locus_tag="ACHA5T_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic GMP-AMP synthase DncV-like
                     nucleotidyltransferase"
                     /protein_id="HFM8886966.1"
                     /translation="MANIQTSFIDFHNSIRLDVEDNTLLKDYKDQVIDGLKDYLPDDV
                     KFETFLQGSYSVYTGIKSCDEKIDFDIDIAVAFEIDHTVYEDPREPKLWVKEALVEIF
                     PNAQVNLKVPCVTATFTGKKTKKNVHVDVAVYAKEDENYFLAKAKEFSAPENRCWEEA
                     DPKVLKEKINSHVADSDDRKQFRRCIRYLKRWKDNNFNQEYKPTGIGLTINVMDTFLV
                     NKSTDFLTRKVQYNDMECMKQIVSSLKDSFVYEYSETDGWHYRLHAYLPVKPNSDTYS
                     KMTVNQMSDFKNKLSKLYDDLIFAIDTEDEYEATKRLNNQFGEDFLIISEEEVTEKNL
                     RNAFVTDYPSA"
     gene            4633..6330
                     /locus_tag="ACHA5T_001384"
     CDS             4633..6330
                     /locus_tag="ACHA5T_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E2/UBC family protein"
                     /protein_id="HFM8886967.1"
                     /translation="MDYTDILKEKLTDVVRLFDGNYYGDFSFATIGKLRLKIEIPKHF
                     PLVLPRIYIHNFDHLGLYLPHVEKDGLVCFSSDINYLVDEENSELVLLQCVNKTIKTI
                     EEGLKGLNKNDFRKEFVAFWDKQENQISLFYLCEPALFIKSVDTIMYGKSLVALDKEN
                     ELQLNAASRLFNGEKEDTHELDAYYVPIRNKNNIMPPKNNKAWSKKDILQIVKKNVTG
                     ANKRFFLKLEKRKNRHNVLLILDIPIEEKSNNVIIGFLFTKQSGSIKDYKGTPRTVLV
                     QRHDELYLVNRTSGENYGLGANIAIVGVGSVGSKIAENLGPLGVRKMTLIDNDCFQAE
                     NLYRNSLGADSLKNARSQNNFFKVDLMQEELEKKIPYLCVEVESMNVLDLISMQPGFF
                     KEYDYVFVCIGDTMASLALNNYFSKSSQKVFYSWLEPYGIGGHTLFINYSLNQKGCFN
                     CLYTDSNSKNRCSNRASLVKEGQIFERTLASCRSKFIPYNAIASTECAINAIKLFHKV
                     NQNNMTNSTLFSWIGDGSTFENLGYQYSDRNTFLNGVNKFEERFFSKYCKVCGELDD"
     gene            6323..6784
                     /locus_tag="ACHA5T_001385"
     CDS             6323..6784
                     /locus_tag="ACHA5T_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HFM8886968.1"
                     /translation="MIKFSCDRIVIEVRDEVLKIFEQYRQGKDDLPEAGGVLHGKRLK
                     NGSIIITDITTPQIGDIQKRLLFKKNREIHQQLSNEKWIESNKFSIALGEWHTHPEAV
                     PTPSNVDKKSWRLNVSKQHDDRVYVFIIVGFSDLRMWLLSKKSDIRQAIKL"
     gene            6797..7657
                     /locus_tag="ACHA5T_001386"
     CDS             6797..7657
                     /locus_tag="ACHA5T_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886969.1"
                     /translation="MKSQKNIWWWGFLVGKVMIPALLLISLPMITFFFTNKKMSKEAV
                     IFFFGDQKATFIETLVTSLQLNLNYIFSIIIVISLLNFFKKRNSGKVFNSNGNVYYNY
                     FYFVFWVAATLLGYDKIQIAGIPIHMQYKLVLSGIFSEVLPDIYDDHYDSDGTCKVSI
                     EKENFDDIDGYDSVNLLIIDTYDIKMSELSMENQTYPTIIVRGNSIDGVRKVNRSLIL
                     EIKKTMDEIQKSDFKKVFVALTSNPKNSINIINSSFRFFGRSRRFKLYVLQKDYASNG
                     KYSKKYRIFI"
     gene            7695..8084
                     /locus_tag="ACHA5T_001387"
     CDS             7695..8084
                     /locus_tag="ACHA5T_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8886970.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLMFV
                     IVFAHVPPLSMIEGAFLKYLGIPIAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            8071..10518
                     /locus_tag="ACHA5T_001388"
     CDS             8071..10518
                     /locus_tag="ACHA5T_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8886971.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDLQTDALVSMI
                     GDSQIDYRFFIGFKLISTDEEVNLKNLRKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVKRVTPSDLTYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPTRCLIEEKPRYLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAKSVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HKEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALASAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVIVELRKEDSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDKEDESNQKCMRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            10523..12631
                     /locus_tag="ACHA5T_001389"
     CDS             10523..12631
                     /locus_tag="ACHA5T_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFM8886972.1"
                     /translation="MKKKLFRIFGIVLISLVTLIILLSLVGTVAEATGLVDDTVGAGN
                     LYSQYSLGNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEVGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMNMFNLQSNDSQSMGRRVMRKPQMLMNRTLR
                     QLNRNVGRTLAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEIPSDLNKEK
                     PIPNNKKQSRMNLAGRKVGKVLDTQALVKDKTKQVKDGVRNTPTNLKYNFHKGIEKTK
                     KAPEEFKRGLVQEKANRGELREKQQQRRDEKIAEKRKVLNEIGNPHGKRRENVPIKAK
                     VQSQKDRAPKRIVRANSNPEIKRKFASQEIISKGINQSSSKKNSFQQVKRRSTLSKRT
                     NRKVQKSLNPHPKPRSGEKK"
     gene            12628..13632
                     /locus_tag="ACHA5T_001390"
     CDS             12628..13632
                     /locus_tag="ACHA5T_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFM8886973.1"
                     /translation="MNVKKLSAISLTFLTISFMGILMCVGLLFGEDSEGSSSSGAPPG
                     GSSVSEEVLKHRAMVEKYAKESDITEYVPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNFGGGYLDYVAKNGKKHSFALA
                     ESFSKEKSCGQKIDYPNPIANKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSRPETGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTESYWQQHLIGAGRYK
                     "
     gene            13651..14562
                     /locus_tag="ACHA5T_001391"
     CDS             13651..14562
                     /locus_tag="ACHA5T_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8886974.1"
                     /translation="MKIKIERNQKEKSLKKKKERVVSVGKHRKMVLALWLLLSCSLTF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGIENFTKDFSKEYFSWKNNKEAIEKRMNNL
                     EQYLTEEGFALSQDMVRADIPTNSEVQSVKVLDVEKSSEEFVVSFLVEQKITEGKKAQ
                     SISSAYRVTIFEDENRNYIVTSLPTMIAKPDRAKYKTKQVENDSKIDAKTTEEIAEFL
                     ETFFKLYPSASEKELEYYVENDAMRPINIDLKFSKLEDPVYLQNGKKILVSTKIKYLD
                     NVSKTTSYFQYKLILQKNGNWKIIDVK"
     gene            14749..15036
                     /locus_tag="ACHA5T_001392"
                     /pseudo
     CDS             14749..15036
                     /locus_tag="ACHA5T_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(15224..15439)
                     /locus_tag="ACHA5T_001393"
     CDS             complement(15224..15439)
                     /locus_tag="ACHA5T_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886975.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(15698..15907)
                     /locus_tag="ACHA5T_001394"
                     /pseudo
     CDS             complement(15698..15907)
                     /locus_tag="ACHA5T_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            complement(16210..17649)
                     /locus_tag="ACHA5T_001395"
                     /pseudo
     CDS             complement(16210..17649)
                     /locus_tag="ACHA5T_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(18197..18868)
                     /locus_tag="ACHA5T_001396"
     CDS             complement(18197..18868)
                     /locus_tag="ACHA5T_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFM8886976.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRYLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCQCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEKWIAYITLK
                     KRGTI"
     gene            complement(19217..20668)
                     /locus_tag="ACHA5T_001397"
     CDS             complement(19217..20668)
                     /locus_tag="ACHA5T_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8886977.1"
                     /translation="MKKSLKLASAVILMSQLFIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTLSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSAESSTTSSSTSEITSSKVTRPNHIESSKKEAPKTEGQTKNFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            complement(20780..>20956)
                     /locus_tag="ACHA5T_001398"
     CDS             complement(20780..>20956)
                     /locus_tag="ACHA5T_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HFM8886978.1"
                     /translation="VQLCSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPY
                     NFILKAVTGLNIET"
BASE COUNT         7006 a         3475 c         4070 g         6405 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaaa aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatcag gaacttacct atccatatat caatggtttt taaccaatgc
     2761 tcaatcttta gtcttactag cgattgtggt cattggtttg tttttaggat ttaaacggga
     2821 gttttccaaa ctgattggct tcttggtgat tgctttgatt gcggtaggtc tggtctttaa
     2881 tgcttctggt gtgaaggatg tcctactcaa tttgtttaac cgaattgtcg gtgcctgagg
     2941 gggaatcaca atggaacaga tgcgtgttta tattgcgaac cttggaaaat acaatgaagg
     3001 tgagttggtg ggggcatggt ttaccccacc tgtcgatttt gatgaagtga aagaacagat
     3061 tggattaaat gacgaatatg aagagtacgc gattcatgat tatgaactgc cttttgagat
     3121 tgatgagtat acgccaattg aagagattaa tcggttgtgt aacttagcgg aggaactaga
     3181 aggaacgcca attggtgaag ctgcttctga gattcaacac gctttcttca attctttcga
     3241 ggaaatggta gagcatgtgg atgatattgt ctattatcca gattgcgatg atatgagtga
     3301 tgtggcgcat tacttgattg tagaagctgg tgattttggc gaagttccag aacaattgaa
     3361 gccgtatatt gattttgatg cttgcggtcg agatttggag attggaggaa attatttggt
     3421 tactagtcgt ggagtttttg agtatccaga atgaatcaaa cactatatat agtgtttgat
     3481 tagtatatgt aagcaaaaaa acacaatata tagagtgcaa actgaagggt aatgtgatat
     3541 aatatttgcc gaagaaatat ttttgaaatg aggtaatatt atggcaaata ttcaaacaag
     3601 ttttattgat ttccataatt caattagatt ggacgtagaa gacaatacgc tacttaagga
     3661 ctataaagac caagtaattg atggattaaa agattactta cctgatgatg tgaagtttga
     3721 aactttttta cagggaagct attctgttta taccggaata aaaagctgtg atgaaaaaat
     3781 tgattttgac atcgatattg cagttgcttt cgaaattgat catactgttt atgaagatcc
     3841 aagggagcct aaactgtggg taaaagaggc gctagttgaa atttttccta atgctcaagt
     3901 taatttaaaa gtaccttgtg taactgctac atttactgga aaaaagacaa aaaaaaatgt
     3961 gcacgttgat gttgcggttt atgctaaaga agatgaaaat tatttcttag caaaagcaaa
     4021 agaattcagt gctcccgaaa accgatgctg ggaggaagcg gatcccaaag tattaaaaga
     4081 gaaaattaat agtcatgtag cagattcaga tgatcgaaaa caatttcgaa gatgtattcg
     4141 ctatttaaaa agatggaaag ataacaattt taaccaagaa tataaaccaa caggaattgg
     4201 acttacaatt aatgtgatgg atacgttcct agttaataag tccactgatt ttttaactag
     4261 aaaagttcag tataatgata tggaatgtat gaagcagatt gtttcttcgc tgaaagattc
     4321 atttgtgtat gagtacagtg aaacagatgg ttggcattat cgtctacatg catatcttcc
     4381 agtgaagcct aacagtgaca cctattcaaa aatgacagtg aatcagatga gtgattttaa
     4441 aaacaaactt tctaaattat atgatgattt gattttcgct atcgatactg aagatgagta
     4501 tgaagctaca aaacgattaa ataatcaatt tggggaggat tttttaatta tttcagaaga
     4561 agaagttaca gaaaaaaact taagaaatgc atttgttaca gactatccga gtgcttaggt
     4621 tggtgttaaa gattggatta tactgatatt ttaaaagaaa agctaacaga tgttgtgcgt
     4681 ttattcgatg gtaattacta tggggatttt tcatttgcta caattggaaa gttgcgttta
     4741 aaaattgaaa taccaaaaca ttttccattg gtacttccta ggatttacat tcataatttt
     4801 gatcatttag gactttacct accacatgta gaaaaagatg ggttggtatg tttttcttca
     4861 gatataaatt atttagtaga tgaagaaaat tcagaattgg ttttattgca gtgtgttaat
     4921 aaaactataa aaacaattga ggaaggtttg aaagggttaa ataaaaatga ttttcgaaaa
     4981 gagtttgttg ctttttggga caaacaagag aatcaaattt cgttattcta cctttgtgaa
     5041 ccagcgttgt ttatcaagtc agttgataca ataatgtacg gtaagtcttt ggttgctttg
     5101 gataaagaga acgaattaca attaaatgca gcgagtagat tgtttaatgg agagaaagaa
     5161 gatactcatg aattagatgc atactatgtt ccaattagaa acaagaataa tattatgccc
     5221 ccgaagaata ataaagcatg gtcaaagaag gatattttac aaatagtaaa aaaaaatgta
     5281 acaggtgcaa ataaaagatt ttttttaaaa cttgaaaaaa gaaagaatag acataatgtt
     5341 ttacttattc tagatatccc aattgaagaa aagtcaaata atgtcataat tggatttctt
     5401 tttacaaaac aatctggatc tataaaagat tataagggta cgcctaggac tgtattagtt
     5461 cagcgtcatg atgaattata tttggtgaat agaacgagcg gagaaaacta tggattgggt
     5521 gctaatatag ctattgttgg ggtagggtca gttggcagta aaattgctga aaatcttggt
     5581 ccattaggtg tgagaaagat gacactcata gacaatgatt gttttcaagc tgaaaatttg
     5641 tatcgaaatt cacttggtgc agatagttta aaaaatgccc gatctcagaa taattttttt
     5701 aaagttgatt taatgcagga ggagctcgaa aaaaaaatcc catacctttg tgttgaagtg
     5761 gagtcaatga acgttttaga cttaattagt atgcaacctg gttttttcaa ggaatatgac
     5821 tatgtttttg tttgcatagg agatacaatg gcaagcctgg ctttaaataa ttatttttcc
     5881 aaaagtagtc aaaaagtttt ctattcttgg ctggagccat atggcattgg gggacacact
     5941 ctatttataa attattctct aaatcaaaaa ggttgtttca attgtcttta tacagattca
     6001 aactctaaaa ataggtgttc aaatagagct tctctggtaa aagaaggaca aatttttgaa
     6061 aggactttag cgagttgccg ctcaaaattt ataccatata atgctattgc ctcgacggaa
     6121 tgtgcaatta atgcaataaa acttttccat aaagtcaatc aaaataatat gaccaatagt
     6181 actctttttt catggatagg agatgggagt acttttgaga atttaggata tcaatatagc
     6241 gacagaaata cttttcttaa tggagtcaat aagtttgaag aaagattctt ttctaaatat
     6301 tgcaaagtat gtggggagtt agatgattaa attttcgtgt gaccgtattg tcattgaagt
     6361 aagggatgaa gttcttaaga tatttgaaca atatcggcag ggaaaggatg acttgcctga
     6421 agctggagga gttcttcatg gaaaaagact taagaatggg agcataataa ttaccgatat
     6481 aacaacacct caaataggag atatacaaaa aagattactc tttaaaaaaa accgagaaat
     6541 tcatcaacag ctttctaatg agaaatggat agaatcaaat aagttttcta ttgcattggg
     6601 agaatggcat actcatcctg aagctgtacc aactccatca aatgttgata agaaaagctg
     6661 gagactgaac gtgagcaagc aacatgatga tagagtttat gtttttatta tcgttggatt
     6721 ttctgatttg agaatgtggt tactgtcaaa aaaatcagat attaggcagg ctataaaact
     6781 ataaaggagt gattaggtga aatcacaaaa gaatatttgg tggtgggggt ttttagtagg
     6841 aaaggtaatg ataccagccc ttctcttaat ttctctaccg atgattactt ttttctttac
     6901 caataaaaaa atgagtaaag aagccgtgat ttttttcttt ggagaccaaa aagctacctt
     6961 tattgaaacg ttagtaacat ctcttcagct taatttgaat tacatttttt caattattat
     7021 tgttatatcg ttattgaatt tttttaaaaa aagaaattca ggaaaggtgt ttaactctaa
     7081 tggaaatgtg tactacaact atttttattt tgtattttgg gtagcagcaa ctttacttgg
     7141 ttatgataaa attcagattg caggaattcc tatacacatg caatataagt tggttttgag
     7201 tggaattttt tctgaggtgt tgccagatat atacgatgat cattacgaca gtgatggaac
     7261 atgtaaagta agcattgaaa aagaaaattt cgatgacatc gatggatatg actctgtgaa
     7321 cttactgatt attgatactt acgatataaa aatgagtgaa ttatctatgg aaaatcaaac
     7381 atatcctacg ataattgtta gagggaattc aatagatgga gttcgtaaag ttaatagatc
     7441 tttaatcttg gaaataaaaa aaacaatgga tgaaattcaa aaaagtgact ttaaaaaagt
     7501 atttgttgct ttaacatcta atccaaaaaa tagtatcaat attattaatt catcgtttag
     7561 atttttcggt cgatcacgta ggtttaaact ttatgttctt caaaaggatt atgcaagcaa
     7621 tggaaagtat agtaagaaat atagaatttt tatttaagaa ggaatttttc cttcttttta
     7681 ttttggagga aactatgaaa aaaataaaaa gctacaccag tatttgggca gttgaaaaag
     7741 tcatttatgc gattaatgac tttcagctgc cttttcctgt gacctttaac cagatggctt
     7801 ggtttgtttt atccttgatg tttgtcattg tatttgccca tgtgcctccg ctatccatga
     7861 tagagggggc ttttttaaaa tacttgggca ttccgatagc ggtcacttgg ttcatgtcgc
     7921 aaaagacctt tgacggtaag aaaccattgg gatttttacg atcctttatc agctatcact
     7981 tacggtttaa ggtcactttt gcaggaaaga aagttaaaga acagaagaaa cgatgggacg
     8041 aaccaatcac cttggtaagg agtgtgaatt atgtacccga ttaagtatat tgaaaataat
     8101 cttgtcttta accaagaagg cgagtgtttt gcttactatg aattagtgcc ttacaattac
     8161 tcctttttgt cacctgaaca gaagtatcaa gtacatgata actttcgcca attaattgcg
     8221 caaaatcgtg aaggtaagat ccatgcctta caaattgcca cagaaagtag tattcgtgcc
     8281 acacaagaac gttcgaaaaa ggaaattacc ggtagactca aagaagtagc aaagcaacga
     8341 attgatttac agacagatgc attagtatcc atgattggcg atagtcagat tgattatcgc
     8401 ttctttattg gctttaaact gatttcgaca gatgaagagg tcaatctaaa gaatctcagg
     8461 aaatctttct tttctggact tcaagaattt gtctatggag tgaatcatca tttgatgggt
     8521 gattttgttt ccttatccaa tgaagaaatt cgtcgctata ccaagcttga aaaactaatg
     8581 gaaagtaaat tggctcgaag atttaaagtc aaacgagtaa cgccgagtga cttgacgtat
     8641 ttaattgaac atatctatgg tgaaaaaggc acaccgtttg aagaatatga attccagcta
     8701 ccaaagaaaa agctgaaatc agaaacgctg gtgaaacgtt acgacttatt acgtcccact
     8761 cgttgtttga ttgaagaaaa gcctcggtac ttacggatgg aacatgaaaa tcatgagtct
     8821 tatgtagcgt acttaacaat taataccatt gtaggggaaa tggagtttcc ttcctcggaa
     8881 cttttctatt accagcaaca acaatttacg tttccgattg atacttcaat gaatgtggaa
     8941 attgtgacga ataaaaaggc cctagctacc gttcgtaata agaaaaagga attaaaagat
     9001 ttagacaacc atgcctatca atctgataat gaaacgaact ccaatgtatt agatgctttg
     9061 gattccgtgg atgaattgga aaccaccttg gatcagtcga aagaatccat gtataaacta
     9121 agctatgtgg ttcgagtgag tgctaaatca gtggatgaat taaaacgccg ctgtgatgaa
     9181 gtgctcgatt tttatgacga taccaatgta aaactagtcc ggccctttgg cgatatgatg
     9241 ggtctacaca aagaattctt accctcaagt aagcgataca tgaatgacta tattcaatat
     9301 gttacgtctg actttttggc gggacttggt tttggggcta ctcaaatgct aggagaatta
     9361 gaaggcattt actttggcta taacgtagat acggggcgca atgtttacct gaaaccggca
     9421 ttggcatcac aaggggtcaa aggctcagta accaatgctt tggcatctgc tttccttggt
     9481 tcgttaggtg gggggaaatc ttttagcaac aatctcttag tctattatgc cgtccttttt
     9541 ggtgggcaag ctgtgattgt cgatccaaaa ggagaaagag gcggttggaa agaaacgttg
     9601 ccagaaattg cggatgaaat taatattctt aacttgacca gtaagccaga aaatcaaggc
     9661 ttacttgatc cctatgtgat tatgaagaaa aagaaagact cagaaagttt ggcgattgat
     9721 atcctgacct ttttaacagg gatttccagt cgggatgggg agaagttccc tgtgttacgc
     9781 aaagccattc gaacggttac ccaaagtgat cagcgaggat tattacaagt aatcgttgaa
     9841 ttgcgtaaag aggactcacc agtcgcggaa aatatcgcgg atcacatcga gtcctttacc
     9901 gattatgatt ttgcccattt gcttttctca gatggttcga ttacccagtc gattagttta
     9961 gataagcagt taaatatcat tcaagtagcg gatttggttt taccagatgc agaaaccact
    10021 tttgaagaat ataccaccat ggaattgctg agtgtttcca tgctaattgt tatttccact
    10081 tttgccttgg actttatcca tagcaaccga gaaattttca agattgtgga cctggatgag
    10141 gcttggagtt ttcttcaagt agctcaagga aaaacgttat cgaataaatt agtgcgtgct
    10201 ggtcgggcca tgaatgccgg tgtttacttc gtcacgcaaa acagtgatga cttgttggat
    10261 gaacgtttga agaataatat tgggctaaag tttgcatttc gcagtaccga tattcatgaa
    10321 atcaagaaaa ccttggaatt ctttggatta gataaagaag atgaaagtaa tcaaaaatgc
    10381 atgcgagatt tggaaaatgg gcaatgcttg atgcaggatt tgtatggtcg agtaggtgtg
    10441 gtccaagttc atccagtctt tgaagaactt ttccatgctt ttgatacccg tccgccagtt
    10501 caagagagaa tggagtgagg agatgaaaaa gaaacttttt cgtatctttg gaattgtttt
    10561 aatcagtttg gtcactctga taatactttt gagcttagtt ggaacggtag ctgaggcaac
    10621 aggtctggtt gatgatacag ttggagccgg aaatctttat tcccaatatt cacttggtaa
    10681 ctatcagttg gatttctttg tggatagttc gtgggattgg ctgccttgga attggggtga
    10741 tggcctaggg aagagcgtaa tgtatggact ttatgccatt actaatttta tttggacagt
    10801 tagtctctac ttatcaaatg ccacgggcta cgtggttcaa gaagcctata agctggattt
    10861 catttcagat acggctgaaa gtattgggaa gaatatccaa accttagccg gcattaccga
    10921 aaatggactc cagacctcgg gcttttactt tgggtttcta ttattgatga ttttagctct
    10981 aggagtttat gtggcgtata caggattatt aaaacgagaa actaccaaag cagtccgggc
    11041 tgtcattaat tttgtgatga tttttctact ttcgggttcg ttcattgcgt atgcgcccac
    11101 ctatatcaca aaaatcaatg attttagctc agatgtcagc gaagcggctc ttagccttgg
    11161 aaccgagatt gtcgtgccga attcagaaag ccaaggaaag gacagtgtgg atttaatccg
    11221 agatagcttg ttttccattc aagtccaaca accgtggtta ctcttgcaat ttgatgattc
    11281 caatatagaa gaagttggcg aagatcgtgt taacaaaatc ctgtctgtaa gtccagatga
    11341 aaataaaggc aaggatcggg aagaagcggt caaagcggag attgaagata atgacaatgc
    11401 gaatctcagt attaccaaga ccatgaaccg cttagggacc gtggtctttt tggtgttgtt
    11461 caatattggc atatccttct ttgtctttct cctaacaggg attatgttgt tctcgcaaat
    11521 tctctttatt atctttgcga tgtttttgcc gattagtttc ttattgtcca tgttgccgac
    11581 ttatgagagt ttgggcaaga aggcgattat tcgcttgttc aatacgatta tgatgcgagc
    11641 aggagttact ttggtgatta cgactgcatt tagcatctcc acgatgtttt tcaatatttc
    11701 ggcaacctat ccatttttca tggttgcttt tctgcaaatt gtgacctttg ccggtatcta
    11761 tttcaaattg ggcgacatta tgaatatgtt caatctccag agtaatgata gccaatcaat
    11821 gggaagacgg gtcatgcgaa aaccgcaaat gttaatgaat cgaacattgc ggcaactcaa
    11881 tcgaaatgtg ggacggactt tggcttttgg tggtggtgca gcagttggaa ataagttggc
    11941 aaaggaacaa tcaaaatcca aatttaagcc atccggttct tctctacgaa agaattcacg
    12001 attaccgaac gatcacgaga taccatctga cttgaataag gaaaagccaa ttccaaataa
    12061 taagaaacaa tcccgaatga atttggccgg gagaaaagtc ggcaaggttc tggataccca
    12121 agctttggtg aaagataaga ccaaacaagt aaaggatgga gtacggaata cgccaaccaa
    12181 cttgaaatac aacttccata aaggaattga aaaaacgaaa aaagcaccgg aagagtttaa
    12241 gcgaggactg gtccaagaaa aagcaaatcg aggagagttg cgagaaaaac aacaacaacg
    12301 tagagacgaa aaaatagctg aaaagcgcaa agtattgaac gaaataggga atcctcatgg
    12361 gaaaagaagg gaaaatgttc cgataaaagc aaaggtccaa tctcaaaagg atagagcacc
    12421 aaagcggatt gtgagagcaa attcgaatcc cgaaatcaaa cgaaaattcg ctagccaaga
    12481 aatcatttca aaaggaataa accaatcctc aagtaagaaa aattcttttc aacaagtgaa
    12541 acggcggtct actctgtcca aacgaaccaa tcgaaaagta caaaaaagtt tgaaccctca
    12601 tcccaaacca aggtcaggtg agaaaaaatg aacgtaaaga aattaagtgc catttcgtta
    12661 actttcctaa caatctcttt tatgggtatc ttgatgtgtg tgggcttatt gtttggtgaa
    12721 gacagtgaag gaagtagtag ttcaggtgct cctcctgggg ggagtagtgt ctcagaagag
    12781 gttttgaaac accgtgcaat ggtggaaaaa tatgccaaag aatcggatat aacagagtat
    12841 gttccgattc ttttagcgat tattcaagtg gaatctggtg gcacaatgga agatgtgatg
    12901 caaagttctg agtcagccgg tttaccaccg aattcattaa gtaccgaggc atccatcaaa
    12961 caaggatgtc tctattttgc gtctttggtt aagcgttcca aagaattagg ttgtgatcaa
    13021 gatagtatca ttcaggccta taactttggc ggtggttatt tggactatgt ggcaaaaaac
    13081 gggaagaagc atagttttgc tttagctgaa tctttttcaa aggaaaaatc ttgcggtcaa
    13141 aaaatcgatt accctaatcc catcgcgaat aaggaaaatg gtggatggcg ctataactat
    13201 ggcaatatgt tttattgctt attggtgaag caatacctaa ctacaacaca gtttgacgat
    13261 aaaacagtcc aagggatttt tgacgaggcg tttaagtatg aaggaacggc ttatgtattt
    13321 ggtgggtcaa gacccgaaac aggatttgat tgtagtggct tgactcaatg gtgttatgcc
    13381 aaagttggtc tgaaacttcc tcgggtggct caagatcagt atgatgccat gacccatatt
    13441 gacctaaaag atgccaaacc aggggatttg gttttctttc attcgactta tgatgccggt
    13501 acttatgtga cccatgtcgg tatttatgca ggggagaatc gcatgtttca tgcaggagat
    13561 ccagtaggct ggacgaatct aacagaaagc tattggcagc agcatcttat cggtgctgga
    13621 cggtacaaat aagagaaaag aggaaaaaga atgaagataa aaattgaacg gaatcaaaag
    13681 gaaaagagcc taaaaaagaa aaaagaacga gtcgtatccg tgggtaagca tcgaaaaatg
    13741 gtgcttgccc tttggctttt attatcttgt agtcttactt ttggcgttta taaaaatttc
    13801 accgccattg atcaacatac aactcatgaa aaagtggtta ttaaagagaa agtagtgaat
    13861 acttctggta ttgaaaactt tacgaaggat ttttcgaaag aatatttttc gtggaaaaat
    13921 aacaaagaag caattgaaaa gagaatgaac aacctcgaac agtaccttac agaagaaggt
    13981 ttcgctttaa gtcaagatat ggttcgagca gatattccca ctaattccga agttcagtca
    14041 gtaaaagtct tagatgttga aaagagctcg gaggaatttg tcgtttcatt tttagtggag
    14101 caaaagatta cagagggaaa aaaggcacaa tcgatttctt ctgcataccg agtgacgatc
    14161 tttgaagatg aaaacaggaa ttatatagta acgagtttgc caactatgat tgcaaaacct
    14221 gatagggcaa aatacaagac aaaacaagta gaaaatgatt cgaaaattga tgccaaaact
    14281 acagaagaaa tcgctgagtt tttggaaacg ttctttaaat tatatccatc agcttctgaa
    14341 aaagaattgg agtattatgt tgaaaatgat gcgatgcgac caatcaatat tgacttaaag
    14401 ttttcgaagt tagaggatcc agtttaccta caaaacggaa aaaaaattct agtgagtaca
    14461 aagataaaat accttgataa tgtatcaaaa accactagct actttcagta caaattaata
    14521 ttacaaaaaa atggaaactg gaaaatcatt gatgttaaat aataatgact caatctaaat
    14581 ttatgagaag ttataagtag tttaacttat ttcaggcaga agtcgcccat cctctatagg
    14641 tgggtgatga atgccgttcg gtaactcgaa aaccgacaat taagacatag tgccttttta
    14701 ttttaatcca tgtcataatc agtctatata agaaatggtt gatataccat ggaattagat
    14761 acaaataatc attcattatt tcttctttgt taccacctta ttttagtagt gaaacattgt
    14821 aaacatctca ttgatgatgt aatgttagct tatgcaaaaa actatctttg aaagaatatc
    14881 agaatcacat cacattacct tagttgagtg aaatcgtgat aaagactact ttcatattat
    14941 gctcaaagca caacctaaaa ccgaattaac aaaattcagc aatgcctaag ctcatgtctg
    15001 ataaaatgag actttctaag ggtcaaaaat ttcctttgaa tacgatcatt agcttagata
    15061 aaagtaaaaa ccttagaact acggggatag cttggttaat aagagatatc gctgttaata
    15121 aagacataag cttgcaagta tgctctattc ccaagaatct cccacttcaa gcactaagga
    15181 tcgttaaatt cagctaagtg gcaagtagtt cactagacga taattatctc catatttcaa
    15241 tctcagcttt tccatttttc atagcaaatt ccgttatttt tatagcatct aaaagattca
    15301 gcttttccac tgatctttca ccacttttca gcttttttat tgtgcttaaa ttgataccag
    15361 taccttttga tattgaattg gaagtaaaat cattaagggt tttttttatt aaattcatat
    15421 ccgcaattcc agcgttcata agaatgaaga ttctcctctc atagactact ttgaaaatgg
    15481 tactacagtg gctgtttata tatttcagta ggttactgta gtggtcattt tattataatt
    15541 agaaaaggaa cctaaatcaa aaatgctaaa gaaaattttc atagtattaa acctatcatg
    15601 actaaagcca cggaattctt gataaaagag tatacttgca agcctatgtc tttactaaca
    15661 gcgatatctc ttattaacca atctatcccc gttgttccta aggttcttga tgttttctaa
    15721 ggcaatgctt gtatacatag cccttgatca aaatattgat actaacgttg acgtttcgat
    15781 catgatgtgt acggcaaata ggacaagtcc attctcgaat tgtgaatgac ttcttaccat
    15841 ctgtgtgtcc acattctgaa ccaatttgac tagacggaaa tcatttatct atcttgatga
    15901 tttttttgac agaagtatcg aaaatcttta cataatctgg gtgttgcatt cttatgcaga
    15961 tgagagcgtt agctccgtat tcataaccag ttgcctctaa aacgtagtcc atcggtggat
    16021 acctagcttg cggttgccag tggctttcgc agtcttcttg taatcaaatc gagcatctaa
    16081 ttgagtacta gtattttgaa gataatactt gagtttttcg gttttcagaa gttcacttca
    16141 tcttccatca tccatggaaa tcccactcac ccatacgcag gcctccaaaa attcttcaac
    16201 aatggttttt cagacatttt tctgaatacc tccttgtttt ttgatacgcc agatagtcag
    16261 gaaaaaggca acaaaacaca aaacaaagct gcctaaaaag gtaatcttca ttccatataa
    16321 aaagacatcc ggccgatctg cgatatagtt agttactcgt tggcccatct tagcagacat
    16381 cgcttgatat aagaccgtcg tagaaagcga aatcccgatg accatcccca aatttcgggc
    16441 aaaggaattc atggagcctg ccagtcccaa attttcgata gtgacagaac tcataaccat
    16501 cgtattattt ggggactgga acaaggcatt gccggcaccc atgatggcag tagcgacaat
    16561 gtacagccac aaaggagagt ccactgacaa aaacatatac atgacttggg ttaccgacaa
    16621 taaacccaag cctactaata caaaaatctc cgggccaacc cggtcggtca ggtatccgct
    16681 taaaggcgcc ccgacgacca tcatcaaggg aaagaccatc atcaacagtc cggcatagct
    16741 aggactcaac ccgcgagcat tttgcagata aaaaggaatc actacattaa caaagaagtt
    16801 ggaagaaaaa atcaacaagg ctgtaatcaa gctcatggta aaaatcttgt tcttaaaaat
    16861 accgaaagaa atcaatggag aggctgcttt cttttcaact tttaaaaagc tgacaaaggc
    16921 aatcactgaa attacgaaca gtacaagggg gactggatgg aaaaagccac tttcttgccc
    16981 caaaaagatt gttccaaaaa ggctgataat cgtagccgca atggcagcaa agccccctcc
    17041 atcgattgta tggccgctca tggtgatatc ttttggtaag aatttttcag caatcaacat
    17101 cgttacgatt cccaccggta cattgatcca aaaaatgtac gcccaagaaa attgggacaa
    17161 aatcaagccg ccaagacctg gtccagcaat cgagcctaat gaaacaagcg caccgattgc
    17221 tcccaatgcc cgaccacgtt catgtatggg aaagacttcc gtgatgattc cggcatttgt
    17281 agccatagtc atactagcac caatggcttg taccacccgt gcgaacaatt aaatacctag
    17341 cgaatgattg aacccacaca acagcgagcc caagacaaag atcagcgtac caatccgaaa
    17401 tactttgatt ttcccacagc tatcgccaat tttaccaaac agcaacaaac aagaacagac
    17461 aatcatcaga taaacggaca ctacccattc cgcttgattc attggtatgc ccatatcatc
    17521 tgagatggta ggtagtgcaa tattgacaat actgccatcc aatgtggaca tgaaggtaaa
    17581 catagatata gcaatcaaaa tccaccagcg atttttttgg acttttgcat cttcttgata
    17641 actatgcata ataggatttc ctttctaatg atcgtgtagc taagaacaat ataacctatg
    17701 tctactgccc aacaacttct cgcgactcta gccataggaa gcttgagtaa tttcgatttg
    17761 ttattctcat gtaaccatgt aagtttgaga tgctttattt gaacttactc tatagtaact
    17821 agtgataatt cgctttactt tcttctagca atttctaaaa gtcgacttca ttgttcttca
    17881 gattaatagt gtgagctatc tcgtgagacg taagaaaatt tccattctac tttttcccaa
    17941 ttttttcata tttatggtgt tgcacctatt tgttttccaa tttgcttttc aaatacattg
    18001 ctatgatatt caattccata ggattatact aaatgtcaat agtatgaata atatattata
    18061 gatgtttata tatcactgtt aattattatc cacaataatt aatgattacc tcgatatttg
    18121 tcaatcaaag gttgaaacaa tttattttca gcatcttttc taacttgagc tgcactctta
    18181 atagaatcat acgatcctaa atagtacctc ttttttttaa agttatatac gcaatccatt
    18241 tctctttaga acgataaact cccttaacac cacttgtatt atttttcggt aattttgacg
    18301 ttagacactc tattactgtc ccttcaacac atgtccttgt ttcttgcctt tttaaacatc
    18361 cacaactttg agtatgtcca aaaaataagt tactataact tatctcacac atactgccac
    18421 actcacattg acaaacccat tttgttttcc atgaacagtt attgtccaaa ggatactttg
    18481 cagtaagtaa tccgaatttt ttattttcaa tgtgatgctt ttttgatttc aaacatccac
    18541 agctttttac atcacctttt agaagtcgct ttgccgatac taactttttt ctcccacaca
    18601 gacattgaca ttcccaaaca acagttcctt cacttcgcag tttagttggc ttgattgcaa
    18661 ctaacttacc aaaagtttta tttgtcaaat ttttatattt atgcctattt ttataatttc
    18721 cacatccaca acttttggtt ctacctgata gtaagtttga tcgatataca atcgtttcat
    18781 tcccacagtc acataggtaa cgatattttt tatatccatt atgagtaatc tcctcaacaa
    18841 cagtaaggtg accatatctt tccttcactg cacctaccct cccaagaaag aagtaccata
    18901 taatttcaat taacttttaa taaatcagaa aaaaatattt tcaatttttc ttagtctgat
    18961 tttatgacta atttataata caagactgaa cgatacgtac acgaaatcct caagatagat
    19021 aaattgtttt catctagtaa aacctgctca aaatatgagc ataaagacgg caataataca
    19081 ctcgatattc gataatggac ttgttttatt tgtcaaactc atcgtaatcg agatatcaat
    19141 tccagtctca atatcctgat caaaggtcta agaatccaac cattttcttg ttactttgtc
    19201 agattgatta ccttttttag tctccgaata ataaggtttt cggatcaact aacatgctct
    19261 gagaaagact actatctttg caaatttcca aatgaaggtg tgggccagta ctattcccag
    19321 tacttcctac atagccaatt atttgtcctt gcaaaacatt atctccagtt tttactaggt
    19381 attgttgctg gtgagcatat aacgcggttg atccatcttc gtgttgtatc accacgtaat
    19441 ttccccaaga gtagtgaaac tctgctttaa caaccttacc agctttaact gcatgaatcg
    19501 gctcaccctg tgtagctgcc atatcaagtc ctcggtgaaa ttcatttcca cgatttccaa
    19561 acaaactggt tattgaataa ttatgaatag gcacttggta tccagtctta ctcatttgtg
    19621 gacctttaac ttctttatca tagttggtta aatcataggt tttaattaat tcatttagct
    19681 tcaaatagta ttgtgtatct gttgcatacc gtcctgtaag gaatttagtt gcttctcgat
    19741 atgttttact attagctttt aatgctcctg cataaaaatc tttatttcca ctaattccct
    19801 tagttaataa tttggcataa tcttcaaaac tttcctcata gttttcatac actctaaatt
    19861 ttgattgggt cgtataaagc gttccattac ccaaatcttc gtttgttacc ataatgacaa
    19921 agtttccatt ataagttcct tttatcccaa ataaattgta attcggtgcc tgagctaact
    19981 ggctttgacc actggcagat tctaagatcg cttgtgcaat cataacagaa gcatataaat
    20041 catatttctg accaattttt ctcgcagact caccaatttt cataataaaa ttttctacag
    20101 attcattttt gtcaaaatga atttcttcat taccagatag tattggttgt gattggaaag
    20161 attctgttga ttctatttgc tgtggtgtcg aaaaattttt tgtttgtcct tccgtttttg
    20221 gcgcttcctt ttttgaagac tctatatggt ttggtcttgt cactttcgaa gaagttattt
    20281 cacttgtact acttgaagtt gttgaagatt cggcacttga aatcgtacta ctttcacttg
    20341 acgaagttgt tgaagactcg gcatttgaag tcgtactact ttcacttgac gaagttgttg
    20401 aagactcggc atttgaagtc gtactacttt cacttgatga agttgttgaa gactcagcat
    20461 ttgaagtcgt actactttca cttgataaag ttgtcaaaag ttcactactc tctgctgtta
    20521 catcggaatt actcatttca gtcgtctcgg ttctttcttt tgcaattttg tatgttgaat
    20581 tttcgcttgc caacacatgg aaaggtgttg ctataaagag ttgggacatt aatataactg
    20641 ctgatgcaag ttttaaagat tttttcattt gatttacctc tcttttttct ctttaatagt
    20701 gttttctatt aaaaattatg ataaatattt tcaatgaatt gagtaataca gtatatttct
    20761 tgatttcatt tagtaacgat tatgtttcaa tgtttaatcc tgttactgcc tttaaaataa
    20821 aattataggg ttggctaaaa ctaggatgaa ataaataatc taacattttc aatttgtcta
    20881 atgtaatttg ttcttctata gcaagagaaa acattgataa tagtccagat aaatcttctt
    20941 gactacaaag ttgtac
//
LOCUS       DAYYGO010000047        20788 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000047 DAYYGO010000000
VERSION     DAYYGO010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.048"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            26..2179
                     /locus_tag="ACHA5T_001399"
     CDS             26..2179
                     /locus_tag="ACHA5T_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HFM8886979.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            2148..3563
                     /locus_tag="ACHA5T_001400"
     CDS             2148..3563
                     /locus_tag="ACHA5T_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFM8886980.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            3596..4630
                     /gene="sppA"
                     /locus_tag="ACHA5T_001401"
     CDS             3596..4630
                     /gene="sppA"
                     /locus_tag="ACHA5T_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFM8886981.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            4632..5153
                     /locus_tag="ACHA5T_001402"
     CDS             4632..5153
                     /locus_tag="ACHA5T_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFM8886982.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            5150..5536
                     /locus_tag="ACHA5T_001403"
     CDS             5150..5536
                     /locus_tag="ACHA5T_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFM8886983.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(6032..6104)
                     /locus_tag="ACHA5T_001404"
     tRNA            complement(6032..6104)
                     /locus_tag="ACHA5T_001404"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6069..6071),aa:Lys,seq:ctt)
     gene            6485..7054
                     /locus_tag="ACHA5T_001405"
     CDS             6485..7054
                     /locus_tag="ACHA5T_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFM8886984.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            7041..7901
                     /locus_tag="ACHA5T_001406"
     CDS             7041..7901
                     /locus_tag="ACHA5T_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8886985.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            8091..8795
                     /gene="yycF"
                     /locus_tag="ACHA5T_001407"
     CDS             8091..8795
                     /gene="yycF"
                     /locus_tag="ACHA5T_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFM8886986.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            8800..10635
                     /gene="walK"
                     /locus_tag="ACHA5T_001408"
     CDS             8800..10635
                     /gene="walK"
                     /locus_tag="ACHA5T_001408"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFM8886987.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            10632..11948
                     /gene="yycH"
                     /locus_tag="ACHA5T_001409"
     CDS             10632..11948
                     /gene="yycH"
                     /locus_tag="ACHA5T_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFM8886988.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            11949..12818
                     /locus_tag="ACHA5T_001410"
     CDS             11949..12818
                     /locus_tag="ACHA5T_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFM8886989.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            12873..13682
                     /locus_tag="ACHA5T_001411"
     CDS             12873..13682
                     /locus_tag="ACHA5T_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8886990.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(13785..14414)
                     /locus_tag="ACHA5T_001412"
     CDS             complement(13785..14414)
                     /locus_tag="ACHA5T_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8886991.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            14584..15876
                     /locus_tag="ACHA5T_001413"
     CDS             14584..15876
                     /locus_tag="ACHA5T_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8886992.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(15952..16614)
                     /locus_tag="ACHA5T_001414"
     CDS             complement(15952..16614)
                     /locus_tag="ACHA5T_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFM8886993.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            16839..17123
                     /gene="groES"
                     /locus_tag="ACHA5T_001415"
     CDS             16839..17123
                     /gene="groES"
                     /locus_tag="ACHA5T_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFM8886994.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            17174..18799
                     /gene="groL"
                     /locus_tag="ACHA5T_001416"
     CDS             17174..18799
                     /gene="groL"
                     /locus_tag="ACHA5T_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFM8886995.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(18888..20027)
                     /locus_tag="ACHA5T_001417"
     CDS             complement(18888..20027)
                     /locus_tag="ACHA5T_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8886996.1"
                     /translation="MWVEQTKDGQFKFIERYVDPYTEKTRKKSTTLTSNSPQAWKKAQ
                     KILDKKIKEALENYNKSDITFGELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHISDD
                     TIVRNIDETLINKMIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPALAVK
                     IHPKKVEEEKRKLSMDKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRYGEL
                     LALQMKDYGEGKISINGTLDYTSVKMDNAIKTTPKNTYSQREVQLSNRAKELIESVIA
                     DNILAGRPTNPDQYIFISTNGTPLTLHSFNAVLHKVEEELELEKNLSSHIFRHSHVSL
                     LSELGVPLKAIMERVGHSDANTTLSIYNHVTKKAKQQVIDKLNRL"
     gene            complement(20138..20722)
                     /locus_tag="ACHA5T_001418"
     CDS             complement(20138..20722)
                     /locus_tag="ACHA5T_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFM8886997.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
BASE COUNT         6936 a         3370 c         4220 g         6262 t
ORIGIN      
        1 ttttagaagg agttgtgtta gataagtgat aaaatcattt ccttttgtgc agcaacaaga
       61 tgaaaaagat tgtggtgttg cttgcctttc gatgatttta aaatatttta aaactgatat
      121 cccgattcat aaattacggg acctatctgg gactgattta gagggtactt cagcatttgg
      181 attgaaaaag acatttgaaa aattgtcttt tgattgtatg gctattcaag cagatagtga
      241 gatctggaaa gaaagagatc ttatcttacc attaattgca catatattgg ttgataacag
      301 ctatatgcac tacgttgtaa tttataaaac gaagggaaac gtactatgga ttgcagatcc
      361 cgctaatgga aaaaatagaa aaacgattga agcgtttcaa aaagaatgga caggtattct
      421 tttattacct atgcctagag aaacttatat tcctattaaa gaaaaagtag ctggattaag
      481 ttcttttttg cctataattt ggaaacaaaa aggattagta ttccacatcg ttttagcatc
      541 gattttcatt acgttttttg gcataggaag ctcatattat tttcaaggta ttcttgattt
      601 tttcatccct aatcaagcac gttcaacttt aaatataatt tccattggct tgatcgctgt
      661 ctatttattt cgggtattgt ttgaatatag tcgtagttat cttcttatta ttttaggaca
      721 gcgaatgagt atggcaatta tgcttcagta tttcaaacat gtgttaactt taccaatgaa
      781 tttttttgct acgagaaaat caggggaaat tatttctcgt tttcttgatg ccagtaagat
      841 tattgatgct ttagcaagtg ctacgttatc aatattttta gatattggta tggtgttgct
      901 tgttggggtt acgttagcta ttcagaatag tacgttgttt ttaatcactc tatgctcttt
      961 gcctttttat gtagttgcaa tattcgcttt cgtgaaaagc tatgaaaaat cgaacaggga
     1021 agaaatggca gccggtgcag ctgtaaattc tagtatcata gaaagtctta aaggaataga
     1081 aacaattaaa gcatatagcg gtgaagagaa agtatataac cgtatagacc atgaatttgt
     1141 taaattaatg aaaaaatcat ttcgtacggt tacgcttgat aatattcaac aaggaattaa
     1201 acatttgatt caattaatta gcagtgcatt aattttatgg ctaggttctt attttgttat
     1261 ggataataga attagtctag gtcaacttat cacatacaat gctttactag tatttttgac
     1321 agatccttta caaaacatta ttaatttaca agtgaaaatg caaacggctc aagttgcaaa
     1381 taatcgttta aatgaaattt tcgcgattga ttcagaaaaa tcgaaccagt cttttgaata
     1441 tatgctttct gaacaattat ttcaacaaga tatcactgta aaaaacgttt cattctcgta
     1501 tagcatgaag gccccaacgt tgaacgaaat ttcatgtaca attccttcgc acagtaaaac
     1561 ggctatagtt ggagttagtg gttctggaaa atcaactctt gcaaagttat ttgttcgttt
     1621 ttatgaagca tcagaaggat cgatccatta tggaaacatt aatcacttag acattccttt
     1681 tcagatatta cgcgatcgag tgacatattt gcctcaagag tcatttttct ttagtggaac
     1741 aatcattgag aatcttatat tcggtttaga tagcccacca agtttcgaac aaataataga
     1801 agtttgtgag atagtccaac taaatgaatt tattaatcaa cagccattac gttacgagac
     1861 attgttggaa gaaggtggaa ataatctttc aggagggcaa cgacaacgat tagccttagc
     1921 acgggcttta ttaaaaaatc cggacatatt aattttagat gaagcaacta gtggattaga
     1981 taccttgtta gaacatgcaa ttatgaatta tctcctcaat ttaaaagaaa aaacgattat
     2041 ttttattgcg catcatttac caatagcaaa agcatgtgat caaattttag ttctccatga
     2101 agggcatttg gtagaaaaag gaacgcacga tgaacttcgt tttgccgatg gtatctatca
     2161 aaaattatgg gaaatctagg agagggaaac aatttatgca gaataataat tggttcgaac
     2221 attctagtat ctatactaaa caacatcata gcttctaccg ttgggtgtta taccctgtaa
     2281 ttcttttttt atgtattatt ggattgtttt taacctttgc taaaaaagag gttgttattc
     2341 ggacaacggc tcaattgaac cccgaaaaaa tagagaagtt acaagtgcca ttagaagcta
     2401 aaataatgga aaacttccta aaagaaaatc aatttgttca taaggacgat gtgttggtta
     2461 gattggattg ttctttaata gagaatgaaa aggctcagat agaacaagaa aaccaacgta
     2521 ttactcaaca aataaagatg gctcagctat ttattgaaag tataagtaaa ggaaaaaatt
     2581 tgttttcaac ggatgacagt tttggctaca gtaatcaatt aaagagcatg ttgtcagaaa
     2641 aagaatcact ccgctacgct ttgaagcaaa gtgaattaaa tgatcaaaag caattagaag
     2701 tatacgaaaa gacaaaaaga caactagaaa aacaaattga gagttcagat agtaaattac
     2761 aagaatggca acaagtacag gtagcttgga gtaataatca atcattaaaa gatttttcaa
     2821 aagaaatgat ggcaaactat gagaattggc aagaacaact aaataatgtt tctgatgatc
     2881 aaaaaaatca agtgaaactg acaatttcag caagcataaa tgaacaaatt gagcaactaa
     2941 aaaaagaagt agaacagtat cagtcagaaa aagctaaatt agttaaacca actacttctg
     3001 agaatgacag aattagtcaa acggaaaaag gaaagcaaga gctagaacaa gcaataacaa
     3061 ctgtaaaaca aacagttaca gagctaaatg aaaaacaaga gaagaaccag tcagttatca
     3121 aaagtttgga cgatcaactt tcaaaaggaa tactaaaagc tcctgtatct ggaacggttc
     3181 atttaaatga agaaacaaag ggacaagcag aactagccaa aggaacagtg cttgcagaaa
     3241 tttatccgat ccacgaaaaa tcaaaaatga aatttacagc acttttacct gcgaatgaaa
     3301 gtacctatat taaagaaggg atgaaggttc attttaaatt ggatcaaaaa ggtggggctc
     3361 cgattacgat cgacggaact ttagatgaaa tttcagaaaa ttctactgcc actgagaaag
     3421 gtgtttttta tgcagtgaaa ggaacgttaa aaacaactca atcgtttccg tttcgttatg
     3481 gattaactgg agagttgtct ttaattgtag gagaaaaaac atattggcaa caaattgtcg
     3541 atgtgtttct aaataaaaaa taattatttt tactaagaat ataggaggag tttatatgaa
     3601 taaaagaaga tggattgctg tgggagtagc gtgcggatta ttgttactat ccattatcgt
     3661 cgcgctaatt ccaggaaaag ataaagagga agcatcaaat acaaccctaa caggaataaa
     3721 taagattttt tacggatcta acgaaatcac agaagaaacg ttagaagaag gcgcatcaaa
     3781 taaaaagatt gtcaaactat ccgtcaatgg agttattgca gatacaggtg aaagcaatct
     3841 ttttagtagg gaacaatata accatcagaa ttttttaaca cagctaaaga aaattcaaga
     3901 agacaaagcg gttaagggag tgttattaga agtaaattca cctgggggag gaatatatga
     3961 aagtgctgag attgctaaag aaatggcaaa aattaaaaag ctagatattc ctatctatac
     4021 tgcatttaaa aatacagcgg caagtggagg gtactacatt tctgctggtt cagataaaat
     4081 atttgctacg gaagaaacaa caactggatc aattggtgtc attatttctg gtttaaatta
     4141 ttctggctta ttagaaaaac ttggcgtaac tgatgcgacg tataaaagtg gtgcattaaa
     4201 agatatgatg tcacctcaac acaaaccttc agaggaagag aataaagtta tacaggaatt
     4261 tgtgatgagt gcttatgatc gttttgtcaa tgttgtagca aaaggaagaa atatggatac
     4321 taatgcagtg aaagaacttg cggatggaag aatttatgat ggtaaccaag cagtagaaaa
     4381 tgggttggtg gatcaaattg gttactctga agatgcatta gattccctaa aaaaagagaa
     4441 gaaattaact gatgcgacgg ttatagaata taaaaatgac acaactggct ttgcttcttc
     4501 ttggttaggt aataagattg ctgaatggca agggctaaag gcaactacat cagatcgtgt
     4561 aatttcagtt tttgaaaaac ttggattttc tgatactcca aaaccaatgt attactatgg
     4621 agggctataa aatgaataag aagagtggca ttgaaacttt gcctaattat gtatttgcgg
     4681 gattttggtt acgatttttt gcttttctta ttgatttaat ttgtattagc atcatcaccc
     4741 atgctactat aggtctcttt tacaaaatca tgggactgga atctgacaaa tccattatta
     4801 ctttttatgg tatagcgtca ttagctattt atttaaccta ttttgtactt ttgactaagc
     4861 tgaattcagg acaaacaatt gggaaaatga tttttgggat aagagtagtg agctttgagg
     4921 aaaaagagct aagttggcaa actatcttta ttcgtgagtg tatatgtagg tttatattaa
     4981 aaaattttgt atttcaaatt gggtatttgg tggctgcttt cacgcccaag aaacaacata
     5041 ttggggatta tttttctaac acgtcagtgg ttgttattcg ttcattagaa gctattaact
     5101 atggtcaaaa aatttctaat tctaaaacac ttaatctgga gagaattaaa tgaataagag
     5161 aaaaacagag tttattttga ttttaattag tggaataaca gggattttat gtagtctttt
     5221 agcattgatg ggtggtatgt ttctatcttc tattgattca actactttaa atactacaga
     5281 actaaccagt aaggatatag atatgttagt caatctaagc gggaatgttg ttttatttgg
     5341 aggaattacc ctagtttttt cgataatgat tttcataata ggttttttag taagaaaaaa
     5401 tcaaaaaaca gtggtctatg gtattagttt actcatttta tctattattc cattttttct
     5461 tttaacatta ttgtgggtaa ttcctggagt tttaggtatt attgcgggat tgatgcttct
     5521 aataagaaaa tcttaattag gcaatgtgta gacaacgtga aacgattaat caattaaaag
     5581 ctaaatacgt tttgagaaat gataacttgt agtcgatgga agacatgtag ataaaaaaac
     5641 tacgtgtctt ttatttattc taattttttc gaaaaaaaga cactctctga ttgttgtatt
     5701 aatggaaaag gggaaagcag caagtaaatt tagtttttct attaaatatt cttcactttt
     5761 taacctttta tagtacttag atttaatcct atatgtttag agagttcatt ggcagcatga
     5821 ttttgcaaag cttctcgaat tattgttata ttagaaactt tactgttcat aattatgctt
     5881 cttttttaac tcaaagtcca aagggctaca gtggactttg agttaaaaat aaaaagccct
     5941 agttattttg aaaaataaaa aaagaacagc gtaaatgttt tcgaaatgct ttcgcaccaa
     6001 catttacctg ttccgtgggt ttggcaccca atgggccgtg aggggctcga acccgcgacc
     6061 cgctgattaa gagtcagctg ctctaccaac tgagctaacg gcccgtacaa aaagtactta
     6121 ataatagtag cactaagaat tttaaaatgc aagactacat ttaaagaaaa tcagaaaaat
     6181 ctttacttcc ttataggaaa tcattcatag attattcaca tttgctcgct attatataag
     6241 cataccaaca aacaaagttc ttgcaagaga acaaacccta acactttttt cattttttac
     6301 tcctttcccg ccgtctcccc ggcgggtctc ctgttcttaa cgataaaaga caagaaggct
     6361 tagccttctt gttttttgtt ttcatggaat cccgctaatc aaatcaccta gttaaagcaa
     6421 ctagattgaa tgtttatcat aagtctgata tcatgtttat gaaatgaaaa aaggagcggt
     6481 tatgatggaa aaaatgcgtc agcaattaat ggagtggggg aaggaactag aaacattccg
     6541 tctgcctcac tgggaagaac tccctgactt agaactttat atggaccaag tcagaacgtt
     6601 agttgatcgt tatctttcac ctgtgataca aggagaaaag catccattgc ttacctcttc
     6661 tatggtaaac aattacgtta aactaggttt gataccagca ccagttaaaa aaagatataa
     6721 caaagaacac gtagcatttt tacttgcaat cactacgttg aaacaagtat tgactattcc
     6781 tgaaatcaaa gaagggatat tgttccaagg aaaaacagta ggaattcgtg aagcctataa
     6841 tctattttgc gatgaacaag aagcagctgt gtggatggtc agccaattag cacaaggtaa
     6901 atctcatccg caaaaattcg accaggcaac ccctgtagaa tatattgctg taagagcggc
     6961 aactttatcg tttgcaatga aacttcttgc ggaaaaaacg attgttctag aaaccgaata
     7021 tttaaaagag gagaaaacaa atgaaaaaca ataaaatcgc aattttagta gattcaggta
     7081 cggatgttcc agaagaattg ataaaaaaat acgagatgta tatgatccca ttgaaaatta
     7141 tttataaaga tcaggtatat acagataaaa ttgacattac ccctgaagag atctatcaac
     7201 gtttgccaga ggaaatccct ggtacatctt tgccggatgg tgagtcgatc acagaaatct
     7261 ttgagcaaat caaaaaagac ggttatgaaa aggtacttgc ggtaacaatt tccagtggat
     7321 tgagcgggac atataatgtg gttcgtttaa ttgcacaaca gcaagaagaa ttggaagcat
     7381 atgtactaga tacgaaaaat attggtattg gtgcaggttt ttctgcgatc caagcagcaa
     7441 aatggctgga agaaggagta gaatgggatc aactgatcag taatctgaat gagttagtcg
     7501 aaagaacaaa agttttcttt aatgttgcga cattggaata tcttcaaaaa ggtggacgga
     7561 tcggtttagt tgcttctatt gtaggaaccg ctttaaagct taacccaatc atctcatgta
     7621 atgaagaagg gatttattac acagtaggaa aagctagggg acgtaaaaaa agtttggata
     7681 aaacggttgc ttacgtcaaa gaaaaaatcg gtaatgcaaa ggtcttcaac ttagctgttg
     7741 cccatgggga tgcgaaagaa gaagcaattg aaatggaagc gagacttaaa agagagttcc
     7801 cacaagcaaa ccaaatctac tttggccaga tttctccggc attagtagtc cacactggtc
     7861 cgggattatt gggtgtcggt gtacaagttt tagaaggata aatcaaaaaa aggaaacact
     7921 gaggtgaaaa tcagtgtttt ccttttttat tgaagttaga acaaaaaata ataaagtatt
     7981 ttgcactttc attttaattt tctcttttgt tttataggca aagatgaaac aaaagcagaa
     8041 atacggtata atgagaaggt atgacaataa tatataagag gtggctcttt atgaaaaaag
     8101 ttttagttgt cgatgatgag aagccgattt cggatatcgt caaattcaat ttagccaaag
     8161 aaggctatga cgtttatact gcatatgatg gggaagaagc cttggaaaaa gttgctgagg
     8221 tagaacctga tttgatttta cttgatttga tgctacctaa aatggacggc ttagaagttg
     8281 ctcgtgaggt acgcaaaact tatgatatgc cgatcattat ggtaacagcc aaagattctg
     8341 aaattgataa agtcctaggc ttagagttag gcgccgatga ttacgtgacc aagccatttt
     8401 ccaaccgaga attagttgca cgtgtcaaag caaatcttcg tcgcggtgcc acggctgcga
     8461 aggaaccaga agaggcagca cctgcggaat tgacgattgg tgatttgacg atccatccag
     8521 aagcttacat ggtgacgaaa cgaggagaaa caatcgagtt aacacatcgt gaattcgaac
     8581 tactctttta cttggcgaag caccttggac aagtgatgac aagagaacat ttattacaaa
     8641 cggtttgggg ttatgattat tttggagatg tacgaacagt ggatgtaact gtacgtcgtt
     8701 tgcgtgaaaa gatcgaagat aatccaagcc atccaaatta tttagtgaca cgtcgtggtg
     8761 tcggttacta tttgcgtaac ccagaacagg agtaattgga tgaaaagaaa agtccgcttt
     8821 tttcgatcag tcaattttaa gatcgctatt acctttattc tgattttatt gatctcgatc
     8881 gaaattattg gtgcctactt tatacgtggt ttagaacgtt ccacgatcaa tacatttatt
     8941 aaagatatga atcaaacggt ggaatcatta gcaacaacaa tcagcccgga attgaatcga
     9001 aaagataatg cagatgatga agaggtaaat gcgaatatca aaagatttat tgaaaacagt
     9061 gcgactagcg atatcatcga aatacgggtc gttgacgaaa aagggatcat ccgaggaaca
     9121 accgatgtca atgaacaaag cgctgtggga aagaaaaatg attatgttga tatcaatgat
     9181 ttcacgacta aacgttatgt tgctttagat aacgacgata aacgggtgaa tatcaatgtt
     9241 caaccgatcc tgtccccgac cggcgatgcc gttatcgggg ctctttacgt caaaagtaat
     9301 atcgaacaaa aatattcgga aatcaataac acagcagtca ttttctttac tgcctcacta
     9361 attgcggcat ttatttcaat gatcgtatct gttttagttg ctcgttcgat tacacagccg
     9421 atcggtgaaa tgagagaaca agccattcga atcgctagag gagactacag tcggaaagtc
     9481 aaagtgcatg gacaagatga acttggacag ctggctgata ccttcaatca gttagctgaa
     9541 cgtattgaag aagctcaaga tacaatggaa gctgaacgca atcgtttgga tagcgtgcta
     9601 tcccatatga cagacggcgt cattgcgaca gatcgcaggg gtaaagtcat tacgatcaat
     9661 gatatggctg tttccttgct agatatcaaa aacgaggaag caatcggaca atcgattttg
     9721 acacttttag atatcgaaga agaatatacg ttgcggaaat tattagaaga tccaaacgaa
     9781 atggtacttg accgttcaac cagtacattg gaaagtgatc agatgatctt gcgagtagac
     9841 ttttctatga tccgtcgcga atcaggtttt atcagcggat tagtagctgt cttgcatgat
     9901 gtgacagaac aagaaaaaac agaacgggaa cgtcgtgaat tcgtctctaa tgtttcccat
     9961 gaacttcgta caccgttgac aagtatgcgt agttatatcg aagcattgag cgaaggtgcg
    10021 tggaaagacg aggaagtagc accaaatttt ttgaaggtaa cgctagatga aacagaccga
    10081 atgattcgaa tgatcaatga cctcttaaat ctatctcgca tggatacagg taatacccag
    10141 cttcaattag aatatgtcaa tttcaacgag ttagtgaatt ttgttcttga ccggtttgat
    10201 atgatggtgg gaaatcagga aaaaaattat aagatccgtc gtgaatttac gcaaagagat
    10261 ctttgggtcg aagtagatac agataaaatc atccaagtag tcgataatat catgaataac
    10321 gcgatcaagt attcaccaga tgggggaacg attacttgtc gcttattaga aacgcataat
    10381 aacgtgattt taagtatcac cgatcaagga ttggggattc ctaaaaaaga tctgaaccga
    10441 gtgttcgagc ggttctatcg agtagataaa gcacgtgcaa gagcacaagg tgggacagga
    10501 cttggattag ctatttctag agaagtgatt aaggcgcatc gaggcgctat ttgggcagag
    10561 agcaaggaag gaaaagggtc gactttttat atctcgctgc catatgaacc ttatgaggag
    10621 gaatggtggg aatgagaatc tctgaaaaag tcgttcgtat aggactcatt ttgatggttg
    10681 cgttaagcat atacttctct tatgcaatct ggctgagccc agcaggaaaa agttcaatca
    10741 acgtagatga gacaaacact caaatgatcg agtctcaaag caatcgaaaa acttccgaag
    10801 tctttcttcc gcttcatgtt acatggcacc attcgggaat gatccaagaa accaacagtg
    10861 aaaatctcgt gtaccgattg caattgatca tcgataaagc aagatttgga aagctttctg
    10921 aagtagtttc aggagacaaa tcacagtttg aacaggtgaa aaatctgaac tctggttttg
    10981 agttggccta taatgcgcct tttttgctaa gagaatacaa ggaagcattt gatttagatt
    11041 tagatgtgtc gaacttggca aaagaaagcg aaaatatgta ttttactaga atacaattcg
    11101 atcaaggaaa taaaaaaatc cgatttttga attattcgaa ccatctcgta tatgaagcaa
    11161 atatttcgtt gaattggaca gatatcaata aagaattaac agcaaaagac acaaaatgga
    11221 cagaaatgac aacagaacca tcgacgatcg acacgcaata tgctacgaaa cattcgatca
    11281 aactgaagaa gtatagttat atcctgtcac agcaacccta tacacttttt agaaatgctt
    11341 ttttccagaa accagatagc gtgaaaaata atgatgaaag caatgaattg attttttacg
    11401 gtgggaatga aacaatgtct atccattcgg aaacgcaagt agttgatttt cgtggagagc
    11461 tgccagagaa aaaagaaaat gctagtgtat tttctgaaag tttcccctat attagcaaat
    11521 taggcagttc tttagggaac ctcagatatt ttgaccgcag tgacaatacg atcgattatc
    11581 ggatattcgt ggaaggcttc ccggtttttg ggacagacag cagaggacag attggtgtgg
    11641 aagttggcgg tgaaacgaat ggttctgtgc aagtgaatat ccaaaccagt ttgaatacga
    11701 ttcaagtgcc gataccttca gacgaagaag tagaacttgc aagtacgaat gaaatcatcc
    11761 agcagttaaa agctaaggga gcagatagca acaagattgg ttcagtgatc gttggctata
    11821 catggcaaaa tgtcaaagaa acgaatcgtg tcgttgatct cttacctgaa tggtatgtga
    11881 aatataaaga taactggtat tcagctaacg accttctgga aaaattacct ggattggagg
    11941 cgaattaatt ggatttcaaa aaaatcgaat ggatattttt cgtcgctttt ttagggttga
    12001 atgtgttttt attcagcagt tatcatgacg cggtatacca agaaaacaat gtcattcgct
    12061 ctaatcagac tgaatcaatc caacaacgtc tggctagcga cgatatcact tacacgggga
    12121 agatttcgtc tgagaacaag caaggctatt atcttagcgc ggagcagacc aatcttagca
    12181 cttcattagc gaattatcgc aaggctggta aaagcggctt gctggcaagt ggtgtgtttt
    12241 tagacggtga tgttttgacg aaatctttat ccagttcaga tgctgaaaag aacgtgatcg
    12301 atccagataa aattagtagt tccttggaag attttttatc taacaaagac aatattttat
    12361 tcggtaatga ttatgtgtac acgaaaaact tttctcattt agaagaagaa ccactagaaa
    12421 tccaagcaag ccaagagtat gaaggcatcc cattcaatga tgatacagca aaaatcgttc
    12481 tttctttaga aaaaaacgat aatgattata gcatcacgaa atacatgcag acgcatttat
    12541 ctgatatcga gcagctgcgc gaaaagacgg aactgtatac ggaagaggaa gcaatcaata
    12601 cattgtacat caataacaaa ataccaagag gatcaaaaat tttatggcgt cagctagctt
    12661 attcctgtat tctgaaagtc cgggaaaaaa atgtgtatgt tcctgtttgg catgtagcga
    12721 tcgaaacaag cgataaagtg gtccaaattg agagcgtcaa cgctttcagc aatacgatcg
    12781 tgacaaacaa tactgtacca aaagtggaag atcaataaaa aagcgagtat aatgaaaaaa
    12841 gtttacaaag taagaaggga agtaggagtc aaatggctga agaaaaacca gcttttaaaa
    12901 tcagtatcct agcaagtgga agttcgggta attcacttta tatcgaaagt gaaaaaaaac
    12961 ggcttttggt cgatgcagga cttagcggta aaaaaatcac ttccttgatg gctcaaattg
    13021 atcggacacc ggaccaatta gatggaatac ttgtgacgca tgaacatcga gatcatatac
    13081 atggcgtggg tgtcttggct cgtaagtatc atttggatgt ttttgccaat cagcagactt
    13141 gggaagcgat ggaaccactg attggaaatg taccagtgga acaaaaacat ctatttgaga
    13201 tggggaaagt cagaacattc ggcgatatcg atattgagag tttcggcgtc tcccatgacg
    13261 cagcggctcc gcaattttat cgtttccata aagaaaatcg gtcattcgtc atgttgacgg
    13321 acaccggata ttgcagtgat tatatgcgag gtgtcattga aaatgccgat ggttatttga
    13381 tggaaagtaa tcatgatctt gagatgttac gcatgggacc ttatccgtgg aatttaaaac
    13441 agcgtatttt aggagataga ggacatcttt ccaacgaaga cggtgcactt gtcatgactg
    13501 atgtgatcgg taatcggacc aaacgaatct atcttggcca tcttagcaag gaaaacaata
    13561 tgaaagaact tgctcattta acaatggaaa atgttttgaa agaaaaagat tttggcgttg
    13621 gacatgattt tgaaatctat gatacagatc cagatgaagc aactgagcta ttttcgattt
    13681 aaacagttca atcaataaaa atataaaaag cagaagataa aatcaatcag ggttggtttt
    13741 atcttctatt tttatttttt tagcatatta aaaaaataac cgtattatcg actgactaca
    13801 ctgatgtgct gcttttcatg tttctcatcc agtatctcat ctactaatgc aatcgcgtaa
    13861 tctgccatgc tgatcgtact gtcgctagca gcgtttcttt gtaaataatc atcacttaac
    13921 aaataagtac ctgtacgttt gccatgtgga tcaaataaag aagctggact gatataagtc
    13981 caatgtagat ttttagattg tttcaatttt aacaatcctt cgtacatatt gaaagctgtt
    14041 ggatagaagg gtgcattttt aggtacttgg tcaatcatac gagtcgtctt ttctgtatct
    14101 aagaataacg aagaggcacc cccaacgaca attaagcgtg tatcggttcc ttcaagcatc
    14161 tcaatcaaat gggacaaaga agtttgatgc atttcttctt ttcctttggg agcgttgaaa
    14221 gcatcaacga caacatcaaa agatgcaaga tcagtagttg tcaactcaaa taaatccttt
    14281 tgtataaacg gaacatctga tggcaatttt tctggatgac gcacgattgc cgtcacgtct
    14341 aaatctctgg acaatgcttc gtctaaaata aatgaacctg catggccagt agctccaatg
    14401 agtgcaattt tcataaaaaa cctccttttt tattcctgtc cattgtaagc agttgctgaa
    14461 tattcgtcaa agaatccgat ttttgaaaag ctcagtgcgc attcctttca tggttcgtga
    14521 tcattgggta tagttaaatt aagaaaactg attgataaaa atagaagttg aaggagataa
    14581 gaaatgaaag agtggttaga tcgtgccttt ttaccaatga taacatctgt tgtgaatctt
    14641 agacccttaa ctgctttgcg taatggaatg acttacacga tcccaataat tattgttggc
    14701 tcgctgtttt tcttatttgc tcattttcct gtggattaca tcgctcagtg gatagacaaa
    14761 acaggatttt ctatgtattt tacacaagta taccaggcta catttgatgt gatagccttt
    14821 tggatcgttt ttgccattgc atacgcttat gcaaaagaag aagacatcga tcgcttacca
    14881 gtcggcataa cagccttgtc tgcttatcta ctgcttattc agccactaaa tactcagata
    14941 gatactgtct ggacaggtgc tgaagggata ctaggagcag ttacaactgg gctcatcatc
    15001 ggatggggct atgcacgttt agtaagactt gcgaaagggt ggaagcaaag agagaagata
    15061 cctgaaaatg tttggcagtc ttttacttca ttgattccaa tcattgttat agtcactttt
    15121 acgcttatcg gttcagcggt ttttcaacat ttgttcagaa tgactgttgt tcaaggagta
    15181 tggacatatg tccagcttcc cattcaatcg ttcatagaca cgttgtttgg tgtgctttta
    15241 ttaggttttt ttgttccgtt tttatggtta tttggtcttc atggatctgc gatggtcaat
    15301 gggttggtta gtcccgtctt gcaagcgaat tcattagcaa atgcagaaat cttagcctca
    15361 ggacaagagt tgacggtggc aaatggcgga catatcgtta ctcagcaatt tctcgatcag
    15421 ttcatgacgg ttacgggtgc tggattgacg ttaggggctg tattttttat gacggttttt
    15481 gcgaaatcga agaaatacag agaattagga aatcttgctc ttttaccagc ctttttcaac
    15541 atcaatgaat caattatttt ttccacaccg atcgtgatga acccgatgat ggctgttcct
    15601 tttattttcg caccggtcgt ttcaggattg attacctatt ccgctttata ttttggattc
    15661 gttcctttat ttacagcagt ccaagtccct tggacaacac cgccgatcct ctcgggtttc
    15721 ttagtaggcg gagtgcctgc agcgatttta caaggtatcg tgttgtcaat tagttttttc
    15781 atttatttcc catttttgaa aaaaatagat tctgcaaact gcaaaaagga aagacagaca
    15841 aaaaatgatg gagcagcaga aaagatgaac gactaaaaaa caaaaatact cctgtcgctt
    15901 tagattggga aaatcaccaa tctaggatga caggagtatt tatagtcatg tttatactag
    15961 ccctttttgg acagctaatt gaacaagaac aactaatgtg ttcattccta cgtgtgtgat
    16021 catcgatgtc cagatccgac cagttgcttt gtactctagg ctgaagaaga aacctaggaa
    16081 aaagtagatt agcagatgac catcattatg agcaaacgca aacagcaaag agctgatcac
    16141 tgctccgacc caaaaatttg aatatcgact gatgatcccc agtatactgc ggcggaatac
    16201 gaactcttcc atgatgggtc cagcaacagt tgtcgccaaa ataaaagcgg gtgcttccat
    16261 gatcatctgg atgatatttt gtgtattctc ggaaggggtt gctttgccaa acaatacaga
    16321 ttctaacatg acagcgatgc tttgcagaat caatgcgaca aagattccaa ccaatccata
    16381 aataacaatc tttggagcag aagctttttt taccgctgat tcgatcgcta agggttcacg
    16441 ttgcttaaaa taaaaaatag ctaggacaac ggcgcctagt atatacatga ctgcagttgc
    16501 tgtgatcaat tcagacgtag attttacaag tcctatcgaa gctaggatca agggtgagat
    16561 aaaaattaga ccatagcaaa aaatgctgag gaagctatat ttttttaaag acatgtaatt
    16621 cctcctttta agggataaca agcatgcaag gcaagcttat atcgattgat ttcaattata
    16681 tcataatttc aataagcagc tgtcagaata aaatttcttt tctttgattt aaaagcttgc
    16741 aaaaaagagg gagaatgagt attattaaaa atgtaggtta gcactcgaag gttaagagtg
    16801 ctaacagatt aaaaaaagag ttatcggagg gatttatcgt gttaaaacca ttaggtgatc
    16861 gcgtcattat cgaagtcgca aaagaagaag aaaaaactgt cggaggaatc gttttagctt
    16921 ctgctgctaa agagaaaccc caaactggga cagttgttgc agtcggtgaa ggccgtttgt
    16981 tagaaaacgg tgaaaaagtt cctgccgctg taaaagcagg cgaccaagtt atgttcgaaa
    17041 aatacgccgg tacagaagta aaatacgaag gaaaagaata tctgatcgtc gcaggaaaag
    17101 atatcatggc gattgttgag taaatttgga agataaaaaa ataatcaaaa ataatcaatg
    17161 aggtgaatca atcatggcaa aagaattgaa atttgcagaa gacgcacgcg cagctatgtt
    17221 acgcggggta gataaattag cagatacagt gaaagtaaca ttaggaccta aaggccgaaa
    17281 tgtcgtacta gaaaaatctt acggctctcc attgatcaca aatgatggtg tgacgatcgc
    17341 aaaagaaatc gaattagaag atcattttga aaacatgggt gcaaaacttg tatcagaagt
    17401 agcatcaaaa acaaatgata ttgcaggaga cggtacaacg acagcaactg ttctgacaca
    17461 agcaatcgtt cgcgaaggtt taaaaaacgt aactgcaggt gctaatcctc taggcattcg
    17521 tcgcgggatc gaattggcaa caaaagcagc agtcgaagaa ttgcataata tctcaactgt
    17581 tgttgattca aaagaagcca ttgcacaagt agcagctgtt tcttcgggtt ctgataaagt
    17641 tggacatttg attgctgatg caatggaaaa agttggaaac gacggtgtca tcacaatcga
    17701 agaatcaaaa gggatcgaaa cagaactaga tgttgtagaa ggtatgcagt ttgatcgtgg
    17761 ttacttatca caatatatgg taacagataa cgacaaaatg gaagctgttc tagaaaaccc
    17821 atatatcttg atcacagaca aaaaaatctc taacatccaa gatattctgc cattgttaga
    17881 acaaatcttg caacaatcac gtccattgtt gattattgct gacgatgtag atggtgaagc
    17941 attgccaact cttgtattga acaaaatccg tggaacattc aacgtagtag ctgtaaaagc
    18001 tcctggtttc ggcgatcgtc gtaaagcaat gcttgaagat atcgctattt tgacaggtgg
    18061 tacagtaatt actgacgatc taggtttaga attgaaagat gcaacaatcg aaaacttagg
    18121 aaatgcttct aaagttgttg tagataaaga caatacaacg atcgtagaag gttctggaga
    18181 aaaagaagca atcgaagctc gtgtgcaatt gatcaaaaac caaattgctg aaacaacttc
    18241 tgattttgac cgcgaaaaat tacaagaacg cttagctaaa ttagcaggtg gtgtagctgt
    18301 tgttaaagtt ggtgcagcta ctgaaacaga attgaaagaa ttaaaattac gcattgaaga
    18361 tgcattgaat gctacacgtg ccgcagtaga agaaggaatg gtctcaggtg gtggtacagc
    18421 tctagtgaac gttatcagca aagtttctgc tgtagaagca gaaggagacg tggcaacagg
    18481 tatcaagatc gttgttcgtg ctttagaaga accaattcgt caaatcgctg aaaacgctgg
    18541 ttatgaagga tcagtgattg ttgataaatt gaagaacgtg gaactaggca ctggtttcaa
    18601 tgctgctact ggagaatggg taaacatggt tgaagcaggt atcgtagacc caactaaagt
    18661 aacacgttct gccttgcaaa atgccgcttc cgtttctgcc ttgttattaa cgacagaagc
    18721 agtcgttgca gataaaccag aaccagcagc gccagcagct cctgcaatgg atccatcaat
    18781 gatgggcggt atgatgtagg aattaaaaga gaacccttat attacagggt tctcttttct
    18841 ttttttgttt tattttaagt aaaaggggca aaaaaggggc agaaatatca aagcctattt
    18901 agtttatcaa ttacttgttg ttttgctttt ttggttacgt gattgtaaat agacagagtt
    18961 gtatttgcat cagaatgtcc tacacgctcc ataatggctt taagaggtac gcctaattca
    19021 gataatagcg aaacatggct atgtctaaat atatgcgatg atagattttt ttctaattcc
    19081 aactcttctt ctactttatg gagtacagcg ttaaatgagt gcagcgtaag cggagtgcca
    19141 tttgtagata taaatatata ttgatcggga ttggtgggtc tacctgcaag aatattgtca
    19201 gctatcacac tttcaatcaa ttcttttgca cgattggata attgtacttc acgttgtgag
    19261 taagtatttt ttggagttgt ttttatggcg ttatccattt ttacagatgt gtaatctaag
    19321 gtcccattaa tggaaatttt accctctcca tagtccttca tttgcaaagc cagcaattcc
    19381 ccatatctca aaccagtcaa atatagaaat tcagctatta tgccgtgtag ttttctgcga
    19441 ggattggaat ataactgttt cagtatttga tcaatttctt ctttctccag atatttttta
    19501 tccatagaaa gctttctttt ttcttcttcc acttttttag gatggatttt aactgctaga
    19561 gcagggtttc tttgaatgta ttttctatca attgcatagt ttagcataac ggatagagtt
    19621 gtttttgttt gctttgtgta gttcagtgag agatcaccaa aagtgtacat atcttcaatc
    19681 atcttattaa tgagtgtctc atcaatgttt ctaacgattg tatcatcgct tatgtgctta
    19741 gaaacatgtt tcatcatcat tggaaccttc aaatagctag tacgtttaac atgctgctta
    19801 taatattcat accattcttt atacagctca ccaaaagtga tatctgattt attgtaattt
    19861 tcgagtgctt ctttaatttt tttatctaga attttctgag cttttttcca cgcttgtggt
    19921 gaattacttg taagtgttgt agattttttt cgtgtttttt ctgtataagg atctacatat
    19981 ctttcaataa acttgaattg gccatctttg gtttgttcaa cccacacttt taacatctcc
    20041 tatcatttgc tataataggc ataacaaata gacctatata ggtttgtttt ctaaaagcac
    20101 gctcttactc tggacggtgg ggcgtgtttt ttatttatta tacagttgct ttaaaaatag
    20161 ctgctttttc aaatgatact aaaggagaga agtggatttc tatttctccc ttttggttta
    20221 acgtgaaatg agcagttaca tccatttgtt ttcctggagc aacagatccc attgtatttt
    20281 cgttggcata tgtctctgat tttttgtcat ctggtccata tacttccaca tctgtaccta
    20341 cagggatatc tgaatcgcca tcatttttta cggtataagt aatttttact acttgtgcag
    20401 gttggttttc ttcaaattga tttctttcat cagttagttc tacactattt agtgtatatt
    20461 cagcatcacc aacttttaca gtgtcaccaa tcttatagaa ggtatcgctt ttttcttctt
    20521 tagaagatga agcagaggtt gaagatttag ttactttttc gccaccatta tcacttgctt
    20581 tattactatc tgatccacca tttaaagcag aaccaataat tataattaaa attactgcta
    20641 atacccaaaa ccatactttt ttgtaaaaag gtttactaac tttatacatt tttccgtctt
    20701 gacccataac tttttttgcc atttaaatat tcctcacttc ttgttataat atatttgcga
    20761 tctcagaaat gaggtatgag tccgtgtg
//
LOCUS       DAYYGO010000048        20599 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000048 DAYYGO010000000
VERSION     DAYYGO010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.049"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..256)
                     /locus_tag="ACHA5T_001419"
     CDS             complement(<1..256)
                     /locus_tag="ACHA5T_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor protein"
                     /protein_id="HFM8886998.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYF"
     gene            complement(246..557)
                     /locus_tag="ACHA5T_001420"
     CDS             complement(246..557)
                     /locus_tag="ACHA5T_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8886999.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            complement(554..1429)
                     /locus_tag="ACHA5T_001421"
     CDS             complement(554..1429)
                     /locus_tag="ACHA5T_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887000.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIKIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            complement(1439..2599)
                     /locus_tag="ACHA5T_001422"
     CDS             complement(1439..2599)
                     /locus_tag="ACHA5T_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM8887001.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            complement(2599..3285)
                     /locus_tag="ACHA5T_001423"
     CDS             complement(2599..3285)
                     /locus_tag="ACHA5T_001423"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFM8887002.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            complement(3248..4426)
                     /locus_tag="ACHA5T_001424"
     CDS             complement(3248..4426)
                     /locus_tag="ACHA5T_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8887003.1"
                     /translation="MGIFQKAVGYFTKKATVTLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            complement(4446..6140)
                     /locus_tag="ACHA5T_001425"
     CDS             complement(4446..6140)
                     /locus_tag="ACHA5T_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFM8887004.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            complement(6118..6432)
                     /locus_tag="ACHA5T_001426"
     CDS             complement(6118..6432)
                     /locus_tag="ACHA5T_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFM8887005.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            complement(6535..6816)
                     /locus_tag="ACHA5T_001427"
     CDS             complement(6535..6816)
                     /locus_tag="ACHA5T_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887006.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVDIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            complement(6821..7165)
                     /locus_tag="ACHA5T_001428"
     CDS             complement(6821..7165)
                     /locus_tag="ACHA5T_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFM8887007.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            complement(7191..7358)
                     /locus_tag="ACHA5T_001429"
     CDS             complement(7191..7358)
                     /locus_tag="ACHA5T_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887008.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKESNEV
                     IPLYGKRIAKH"
     gene            complement(8042..8455)
                     /locus_tag="ACHA5T_001430"
     CDS             complement(8042..8455)
                     /locus_tag="ACHA5T_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFM8887009.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            complement(8531..8827)
                     /locus_tag="ACHA5T_001431"
     CDS             complement(8531..8827)
                     /locus_tag="ACHA5T_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887010.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTREKPKFKVGDMVRCEA
                     EGFIYPFRGYVEKVLSNSAIIRIENTMECDKWLAKSKENLAVARLVDMEVIESK"
     gene            complement(8827..9033)
                     /locus_tag="ACHA5T_001432"
     CDS             complement(8827..9033)
                     /locus_tag="ACHA5T_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887011.1"
                     /translation="MKIYVVKFGNQFYRSDERSIGANTLSIVDILQSARWFDNLEEAN
                     QVSRRLGGLTQVYELVTVDHEGVE"
     gene            complement(9030..9227)
                     /locus_tag="ACHA5T_001433"
     CDS             complement(9030..9227)
                     /locus_tag="ACHA5T_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFM8887012.1"
                     /translation="MNNRHRRVAKLRNQELNVAKVKFEREYGVSAEETYKVASQCVAD
                     ASDAIRKFGISILDIKWEDTE"
     gene            complement(9251..9556)
                     /locus_tag="ACHA5T_001434"
     CDS             complement(9251..9556)
                     /locus_tag="ACHA5T_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887013.1"
                     /translation="MNVQVYLKRDLARFPRSWDTNSHPSEAEEEGYLMSANMIEITRD
                     DADEFDKKRSWYSVPNPMYTAVIEDYHISDRFILIDLERPKKRIKQYRRCGFSTKKW"
     gene            complement(9553..9726)
                     /locus_tag="ACHA5T_001435"
     CDS             complement(9553..9726)
                     /locus_tag="ACHA5T_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887014.1"
                     /translation="MSRQIMLHIPDGTKAMTITIVAAQKDGGLALKTKSIDTKQILEG
                     RDVEIEIDEEEAE"
     gene            complement(9723..10292)
                     /locus_tag="ACHA5T_001436"
     CDS             complement(9723..10292)
                     /locus_tag="ACHA5T_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFM8887015.1"
                     /translation="MNKQEAIKKLESIKVIGNDALAACYNESINAGITLMKKIDEPKK
                     LVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLYSVIIAGEYLVKEISCSNEVKLVSTAYLPQYLAH
                     HYQLTEKQIKAIDERYWPFAVPVEEVAEG"
     gene            complement(10304..10438)
                     /locus_tag="ACHA5T_001437"
     CDS             complement(10304..10438)
                     /locus_tag="ACHA5T_001437"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887016.1"
                     /translation="MKDWLEEASALVGCFINCAFISVIIALVGFVSLKVIILLWQLIF
                     "
     gene            complement(10438..10773)
                     /locus_tag="ACHA5T_001438"
     CDS             complement(10438..10773)
                     /locus_tag="ACHA5T_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887017.1"
                     /translation="MIILATDYIDSLKDENGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDIHGKSKSAIKLFGAIEIYADDF
                     PNLWLKEEQ"
     gene            complement(10770..11156)
                     /locus_tag="ACHA5T_001439"
     CDS             complement(10770..11156)
                     /locus_tag="ACHA5T_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFM8887018.1"
                     /translation="MIPRFRSWYTPFKGKTIGQEMKYGQAGRLITHAEMAPDKYVLMQ
                     STGLKDKNGVEIFEGDVVLVNVSNGFDHLVNEKTVVQESEFHSGLVCKSLISGMEYRI
                     FSHQKMGYEYEVTGNIHENSELLEVE"
     gene            complement(11153..11470)
                     /locus_tag="ACHA5T_001440"
     CDS             complement(11153..11470)
                     /locus_tag="ACHA5T_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HFM8887019.1"
                     /translation="MNLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILDEYME
                     GSK"
     gene            complement(11470..11775)
                     /locus_tag="ACHA5T_001441"
     CDS             complement(11470..11775)
                     /locus_tag="ACHA5T_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887020.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWTEKTR
                     RRMPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRAKNMDIDSKGRQFRYVEEK"
     gene            complement(11772..11933)
                     /locus_tag="ACHA5T_001442"
     CDS             complement(11772..11933)
                     /locus_tag="ACHA5T_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFM8887021.1"
                     /translation="MTTEEVIQMRIRSIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            complement(11948..12307)
                     /locus_tag="ACHA5T_001443"
     CDS             complement(11948..12307)
                     /locus_tag="ACHA5T_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HFM8887022.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQV
                     PLILAKKYRNTFKEERF"
     gene            complement(12304..13155)
                     /locus_tag="ACHA5T_001444"
     CDS             complement(12304..13155)
                     /locus_tag="ACHA5T_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8887023.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSFVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            complement(13172..14005)
                     /locus_tag="ACHA5T_001445"
     CDS             complement(13172..14005)
                     /locus_tag="ACHA5T_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HFM8887024.1"
                     /translation="MSDNKRYYYLKLKENFFDSDEMVLLESMPDGYIYSNILLKLYLR
                     SLKHEGKLMFNDRIPFNSTMLATITRHSVGVVEKAVQIFRDLQLIDVLDNGAIYMSDI
                     QSFIGKSSTEADRKREYRKKIEEAKRNLITGGQVSDKCPDKTTPELEIEIEKDIDIDK
                     EEKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEY
                     VINWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKNNHKKQSQKLQYSNPSEYDDLP
                     I"
     gene            complement(14012..14698)
                     /locus_tag="ACHA5T_001446"
     CDS             complement(14012..14698)
                     /locus_tag="ACHA5T_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFM8887025.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIGATINEEVNIDRLKTMYDGYDGD
                     RIIEVRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHNIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            complement(14704..15450)
                     /locus_tag="ACHA5T_001447"
     CDS             complement(14704..15450)
                     /locus_tag="ACHA5T_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFM8887026.1"
                     /translation="MSEKEQPLKNRSDNTLFNTLYKINVKDVTEKRNNLTYLSWAWAW
                     AEVSKVCEAVDYEIYHDPETYLPYVFDKKTGYMVFTSITVNGVKRDMWLPVMDGANKA
                     MKDEPYTYEVNDYQWNNETKKKEIVGKIEKRVEAATMFDINKTIMRCLVKNLAMFGLG
                     LYIFAGEDMPEDVSMLEPATQRSKKLFLDALQLVANKYEKSIDEAIVALTDAASITAD
                     DSKWTKRDLGILKRGVNWIEDQYREETKEK"
     gene            complement(15443..15784)
                     /locus_tag="ACHA5T_001448"
     CDS             complement(15443..15784)
                     /locus_tag="ACHA5T_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887027.1"
                     /translation="MKKELSTLDQYLTDPSWGKSNIKETSNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWDHVYLIHLSRKSKKTEYIYVIEDGLIDALEEYDRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            complement(15842..15970)
                     /locus_tag="ACHA5T_001449"
     CDS             complement(15842..15970)
                     /locus_tag="ACHA5T_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887028.1"
                     /translation="MNRAEALKIGVIIANRWWRHNKPNILSQQHIDKQKAWQQIKK"
     gene            complement(15963..16670)
                     /locus_tag="ACHA5T_001450"
     CDS             complement(15963..16670)
                     /locus_tag="ACHA5T_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFM8887029.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFDKKHRHVLTAIDELKEGVAEN
                     WADLFWEDTYVHPQNKQSYRIIYMNRDGFSLLAMGFTGKKALSFKLQYIEAFNKMEKE
                     LKDQLPSIPATKREMALLALAANEETNERVDAIESDLNDLKNNQLLAEPDYRTISNMV
                     RNKIRVICNQQHLNSKAKAELFKDLNGGIKRITGAVARNRIKAKQFDDVIDFINNWMP
                     STATMTIIKQMELLEDE"
     gene            16715..17194
                     /locus_tag="ACHA5T_001451"
     CDS             16715..17194
                     /locus_tag="ACHA5T_001451"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887030.1"
                     /translation="MSRKIDNIILLTTIFYLVIGATLSLLSFLSNSSLIVLNQKSILT
                     EFAHIDYFSFFKTGLICLFLSSFILYIGTTFCLMVLNLKMFIIEKWIVIIAKIFSSIS
                     IIIPMTLTFSKEQFNVATTFVSFIALFSFAVPKTFQSKSSNAKVNVENTDRKNDESY"
     gene            complement(17253..17423)
                     /locus_tag="ACHA5T_001452"
     CDS             complement(17253..17423)
                     /locus_tag="ACHA5T_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887031.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFIGLDE
                     AEYKTKKPVGRD"
     gene            complement(17423..17722)
                     /locus_tag="ACHA5T_001453"
     CDS             complement(17423..17722)
                     /locus_tag="ACHA5T_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887032.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKSLSPSDVLKEAS"
     gene            complement(17827..18030)
                     /locus_tag="ACHA5T_001454"
     CDS             complement(17827..18030)
                     /locus_tag="ACHA5T_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8887033.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(18045..18791)
                     /locus_tag="ACHA5T_001455"
     CDS             complement(18045..18791)
                     /locus_tag="ACHA5T_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFM8887034.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
     gene            18869..19177
                     /locus_tag="ACHA5T_001456"
     CDS             18869..19177
                     /locus_tag="ACHA5T_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887035.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            complement(19148..19405)
                     /locus_tag="ACHA5T_001457"
     CDS             complement(19148..19405)
                     /locus_tag="ACHA5T_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HFM8887036.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(19703..19843)
                     /locus_tag="ACHA5T_001458"
     CDS             complement(19703..19843)
                     /locus_tag="ACHA5T_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887037.1"
                     /translation="MEKTVSAKIEDLKLDILKQAKVAMEHAVDKEDSAMVAAIAEILA
                     HV"
     gene            20137..20457
                     /locus_tag="ACHA5T_001459"
     CDS             20137..20457
                     /locus_tag="ACHA5T_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8887038.1"
                     /translation="MITFERIKELAKKQGKSLNKVEEDLGYGKNVLYRLKNSNPSTER
                     LQEIADYFDVSVDYLLGREEKETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            20475..>20599
                     /locus_tag="ACHA5T_001460"
     CDS             20475..>20599
                     /locus_tag="ACHA5T_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HFM8887039.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYG"
BASE COUNT         5677 a         4309 c         3096 g         7517 t
ORIGIN      
        1 taaaatattg atctttatta aatttcttgg cgattggatg aaatggagtc tttactttct
       61 tagttaattt cgatcccatc gcatccatag ctgttatatc actatctctc atggaaagat
      121 ttctaaattt taaagaagtg atttctgtat cttctacacc aatctttttt cctagttcat
      181 ttcttttggt agtttgcgtc aaaatcttta accaaccatc gttgaatgtt tcttcgagtc
      241 tacgattatt cgccattcac atcacctgca atatattctt gtagcgcata atgttgaatg
      301 aaacctaata actcactagc gaaattttgt tcaaactcat ctaaagcacg attccagtcg
      361 tatctacatc tttcgattag caatccgtat tctaagcttt caggagaaaa agaaagtgtt
      421 gtactcactt tactttgaag ataaacagca tttttagcta tcatcttttt aattgactca
      481 tcttcttcgt tccaggtaac gtaaatatta tccttcacag ctattagcaa ttcttcagtc
      541 acttgttcag gcgtcatcta accaccgcct taattgcttt aacatatgcg taagagcatt
      601 ttttcttgtt tacaaatgat aaatcttcat caaaaggcgt agaagtcacg tatctccctt
      661 tgaaaaataa atcttcatcg tttgttgtta ctccagcatt gtgtaagatt tttacttctt
      721 taactttttc tattggatca gtagcaaaac aaaagtctaa ttcctcgtga actttaggac
      781 caatattgaa atacatcatg ttccaaagct gtgcccacat ctcggctgtc cagattttta
      841 tatttgtttt ttgccctcta aggtagcgat atagccgatt agaatccaga taaacctttt
      901 tccaataatt cgctttaggg cggttaataa cccactgtgc gcctcctgaa ttagtgttta
      961 tagtttccaa agattctact gtaacattta caatgtttgc catatctttt agaatatttt
     1021 ctccgttttc acagcttcta atataatcaa gacttagata actacagcag tcgctacaat
     1081 accaaacatc atctttagaa ggcaatttgc gcaaattaat tcttttattg aaaatgacat
     1141 ccgaatcgat atagaaatat cggtcgtcct cacgcgaatg atcttcttct aaatatttcc
     1201 accataaata tggtttaatc gaaggaatat actctttgtc gtcccgcaga tcatcgtaca
     1261 catgaacttc aacaccatat tccttctcaa aaaaaatagg aatctgatca tcgtgtctgc
     1321 tgaaaagcaa tacgatatct ttgattccta gtttcttcag attagttaaa caaacttcaa
     1381 gctcccattt aaaccgattg attgccggct gacaaagaat atacttcatt ctgatcacct
     1441 acgcttgtgt tgtagttgtt gtggttgttg gttttgtagt tgtagtagta gttcccaaag
     1501 cgctaatatc taatacaatg aaactatcgt tacgtttagg ttgaccgttt gcatattgtt
     1561 tagctagata aatgcgttcg tcttcaacaa aatggtattc atctgaagct tcaattttta
     1621 gtgtagatcc tacacccatg aagtaatctg aggctacccc aataactgct tttccttctg
     1681 gcacagccgt tgactgcaaa tctgaaactg gtactggcaa tacttgtacg tattctccat
     1741 tagcagttag tacagtctta gctgggaata ctttagacca gtaatcagtt ggattcacaa
     1801 ttaggaccac atcagaagga ttcacattac gataaatcgg atcattcaca ccttcgatat
     1861 tgaattttga tagtcgcgcc atcaaaccgc ccatagttac agcatctaaa gctgtaatag
     1921 gttctgcttt tttttcagca tattctccgc tagtttgttt gctcatgtca cgcatcattc
     1981 cgactggcat atctttacca gtaccatcaa caattgcttg ttctaatgca attctcaatg
     2041 attctactaa aacagtacgg acataacgat ctaaccatac tggacctaaa tcaagcattg
     2101 ccttacatac aggaatataa cctgatagct tgaactgctt catgttaatt acatcaaagc
     2161 cattatctaa aactttttta acagcttcgc aaagtttacc ccaccatgct ggattgactc
     2221 cacgtgacac aatccattct gttacaccag ttgtgttaac aaaagtaatt ttttgcaata
     2281 gtggatgaga ttgttctaaa tcttcaaata cacgttcaaa tacagtagct ggcactaatt
     2341 cttcgacccc tgcaaaacct tcgtttttca ctacttcgtt atagaatttt gtttcttgtg
     2401 tagttaatac acgctgacca cggttcatta atactaattg atcttgattt tttgctgttg
     2461 cttcttctaa aattttatcc tgaatttcct tagataagct taccatagct gcgctaaaag
     2521 attcttcgtt accatcttta aaagctttca tcaattggtc gcttgcagct gttacacctt
     2581 ttaaattttt aactgtcatt attttacatc tccttgtcca aatgttttat ttaatgctgc
     2641 tgtaaatgca gcaatttttt ctgctctttt ttctttaacg tcattcaaaa tttcttcaac
     2701 gctttgttct tttttagctt cagtacctga gctattttct gcatcgataa tttcatcgac
     2761 caatccataa ctcaaagctg tttctgcatc cataaacgat tctttttcaa gaagttcttg
     2821 caatgcttca tctgtgccat tgaatcgtgt tttatatgaa gcctttaccg atttatcaat
     2881 tgattccagt tggtcagcaa tcgtacggaa gtcatcgaca tttccttctc cgtatgtgga
     2941 agcgcggtga atcatcaatt gtgcattgtt gtagattttt atagtatcgc cagccattgc
     3001 gataattgaa gcagcactag cggctaagcc gttaatcaca acgttaactt ttgctttatt
     3061 tgacttaagt aagttcccaa tagcaatccc ttgaaatacg tctccaccgt ttgaattaat
     3121 tactacttca atttcttctt gatcacctag actatccaaa atatttttga ttcccttgtc
     3181 agtattccct tcaaagaacc aactagaacc aataaatccc tgaataaaaa tttgcggtac
     3241 tgcgccttca ttctttactg ctagaaatgt tttcattgtc gtcattcgcc tcacctcctt
     3301 tcgatacttg ttgattgttt ttagttataa atatttcatc tgccatcgcc ttatcagagc
     3361 gatcatttcc aacgcgttct cttccttcgt tgattgtaaa tactccattt ctaatgccta
     3421 catcaatagc gtcaaccaaa tctttgaagc tagtaatctt gatcatagtt gtatccacac
     3481 gtacaaaatt ccctgacaag tattcttcta cttcatagag actagcgtta aacgcatcct
     3541 gaataagttc agcaatcggt atgatttcga acattaaaaa agcgtccact tgatccgata
     3601 acccactcat gtctcccttt agtaggtttt tcggaacgtg aaacgctgct gctgtcatct
     3661 caaagatgtc gtctattaag ttttttatat ctcttgaatt gctttggaag tttccgctga
     3721 aatcttctaa tgtgtactca ttttgtaatt gaaataccgc acctgcatta tcagcttcca
     3781 taaaagcctt aaattgtgat gtcatcattt tattgatttg atcttgtgtt gtattgtctt
     3841 gcggtcggaa taaattccct ttcagtacgt atctacgagc gttagagcgc ttgtaaacat
     3901 tcatggcact agaaatgagt ttcccatacg cttgataata cgcatcgact agttgcctaa
     3961 tttgttgatc tgcgtatttt atatagataa catcactttc tagaaattct ctatcaagga
     4021 ctatgttgtt aatttgcact tgagaaaaca catcatcttt caatgcatat tctgtgacat
     4081 cccaactatc cgcaataaat atttcgctag aattattaga cggagaaacg atcaatactt
     4141 cattgtagaa tattaatctc ctgatcagtt tttttctaaa ttctgttgca ttatttttct
     4201 tattaggagc tacattcagc ctatagtaaa gatcattctt tttatttttt ccatcttcat
     4261 atgacttgaa ttccgcttta ctcatcgcat ttgcaatcaa atcaatacaa gtttcaatcg
     4321 caaattttcg atacacaaaa tcaacttgca atttacaaaa gtattcttct aaagtaaccg
     4381 ttgctttttt tgtgaagtat cctaccgcct tttgaaaaat ccccactttc tcacctcctt
     4441 tcaagttaga atactagagg agtaaatcca gttcctgtat tttctactga gctatttgtg
     4501 actgttacag gagcagaatc ataaatatca tctaaaaaat tcaaaccatg aaggaatgaa
     4561 aaaaagccat ccgtttttct agtttcaggt tctatttttt catagcgtat atttccatta
     4621 gaaatatgct cttcatatac attcatgcaa taccaacgca taatcgcatc gtcaccaaaa
     4681 aataaacgtt gattaataaa aaggtcatca accagatctt ttaacatacc atgtgtaaca
     4741 gatccgcttc gaacaatttc cacagtaaaa cctgcttctt ctaaagcggg cttcaatatt
     4801 tttgcacggt acatatccat agcgattttt ttaatataat atttattact catttcaaga
     4861 aaccaaccta caatataatc agcttctata ttttttccat gaacgatctg tgattttcct
     4921 tgatctatag aaatatctat aacctctcgt ttgatgtttt gtaatcgaag ggctgattcg
     4981 tggataaaag tatgttgtgt aaaataaaca tctttatcgt attttcctag caacccaacg
     5041 ctggcaaaat ctcgtctatc agcaaaatcg actgttccta tcacttcatc cattttttca
     5101 ggaaattctt tttctttcgt atgcagaaca tcatcatatg aagcaacagc aaatcgtgta
     5161 tcttccatag gtctgttcat tcgtttggtc atgaacgtaa gtcttaaacc agcattacgt
     5221 tgcatttgag agtattcttg aaacattttc cgttttaaat ctgcattgta attaatagtt
     5281 ggacaagctt tttcccacat gtcgggatca tcaacttcat tatcgttatc caagcgacaa
     5341 ataaatggaa acaaactaga aaattctgct ccatccttgt caattccaag ttctccagaa
     5401 agaatcattt ttgattcttc tataatgtca tcaagcggac caccacgaac atgaccatta
     5461 gttgtatcat aaaattctct ataatctcga attttaccac caccagaagt agccacattt
     5521 atcattgaat aatcttcatt ttcgtgaatt tcatcaaagc ggtttgcacc tggtcgcttc
     5581 ccatcttttg ttctagcatt tgccgtgtta taacgaagtt tgctgtttgt agcgatattt
     5641 tgaataactt ccttcgtagc tttaaatact tttttatcta aatcaggatg atctttaatt
     5701 actttaaata catcatcaaa actagtcttt gcttggcttt cattattggc atagatatca
     5761 atatcataat ttttaatacc gtgtttagcg gttagtagaa agaagttgtt ccaagaagca
     5821 aaaccagttt taccattacc acgtcccatt aatgaaagat atctattgaa cactagtgtt
     5881 ttatcttttt tccatcgaac accataaata aaacattgta gaaatttttc ccacggaatt
     5941 aattcgaatg gaaagtattg tgctggtata ttgattgaat cctctaccat ctgcttatcg
     6001 aagtaaatat cttctctagt aaagactctt tcttctagat aattttttag caataattgc
     6061 tctttgcata ccttgatagt gccttcttct atagctttga accaattttc aatatgctta
     6121 taactcagga attgattcat ttgcttcacc taccaattca ggagtaatgg caagtttatc
     6181 caacatcaat cccatttgtt tgttgacaga aacaagcaac gctactgatt cattcttttt
     6241 accattctcc agtctaatgc cgttctcgga tatatcttct tccagtgata tcgccgtttc
     6301 ccataaactg atataacgat caacattatc taagaatggc tcaatatttg ttttctgact
     6361 ttccaattgg cttattaaag agcggcgtaa tttttctctg tagcgatttt gagacaattc
     6421 gtttttaaac attttagccc tcctttcatg ataaagttcg aaaaaatctc ttttcctgac
     6481 agccccctcc gtttcatcac ccccaaaaaa tttgcgattt attttaaggg ggggttatct
     6541 caccatcgga atgaaagctt cagcgaagtc aatgtaataa ttaatctctt caatactata
     6601 tccaaaaaca ttttttattc tttcgacgtt attatcttta ttcaacgctt ctcttacttg
     6661 attcactttg tatttactac aacagttatc tgataacaga tccctaatat ctacatagcg
     6721 aacgtatatc aaacgtttaa ttaatccctg agtataagat gaatactctt caatcttttc
     6781 tgtatcatac tctctgccat tatcattgat gatcatgcac ttaccacctt tcacttgcat
     6841 cgaagttagc aaagctttct atcttcttct cttgtttatc taatgctgta agatatctgc
     6901 catgaacttc attatgatgt tcaacacata aacaaataag attatctaaa tctaaagcta
     6961 agtcaggtct atccttgact tcctttatat gatgaacgtt ttctactcta tgatacttac
     7021 ctagtcttct acactcttgg cattcatagt gatctcgttt catcgctttc tctctaagcc
     7081 tgcgccattt aggagactga tagaacttaa ctaaacgatc ttctcttatc aactgtaata
     7141 gccatctata gaattcctcg gtcatattcc gtctcctttc gcaatcttat ttaatgctta
     7201 gctattcttt tgccatacaa tggaataact tcattgcttt cctttcgttt gtatgtatcg
     7261 ctctttattg gtcttctata ctttcgtact atctcaccgt taccgttttg cacagtgatt
     7321 acttcatact tctgttctaa gtattgtggt ctatacattc ttgttacctc ctttgtgtaa
     7381 aataaaaaga ccactcaaag agtgatctaa tctgtaaaaa ctacacctca taaacgagat
     7441 gcagttatag ctctatcaag caacctacac cgattccatc gattactatc gacctcgcct
     7501 tgctcgtgta ctttgagcgc ccatttccaa ccctcggttg ccaaagtcac tggcaatgaa
     7561 tcgaacattg catggtcaaa tcataaaacg ttaaggctat ccctcgacgt attgacctta
     7621 tttttaagcg tctacccttt ccgccacagt gacactataa aattattctt ggctgctact
     7681 attttttatt ttgcccattt ttaaatccaa tcatatagac attaagacag agcgcaaaaa
     7741 ttgaaattat taatggaatc atttctcttc actcaccttt agttatcgtg tgaataatta
     7801 aaaaaacaat agacagcaac ggatgataga taataagaac aatttagaag gagttgaaat
     7861 tcacatcctt attcttaata tttccgttgc tgtctatcga agcttaattg tgaaacaata
     7921 ataaaacgat gttcctttta ttattatttt gtctcagacc tatcactaat ctttcgacac
     7981 taccataata tcactggtaa atagctaaaa accgccatca ttccgccaaa aaaccgccaa
     8041 attatttata agcaattatt cttccgtgtt tatatgcttc tgcaaactct attagagctt
     8101 ccgacttcat ccgttgtata cttctttctg aataacccac ttcacggcta attctgtagt
     8161 ttgagaagct atctggcaca caaaagctgt agtagagtat ctgacggcta atcagactaa
     8221 gagccatcaa agccgctaaa atcgcatctc tctctgcttc tatatccatc atctgaatga
     8281 tcgcgtcctc tgccttattg ccgtgcttcg gcgccttcgg catatccgta ataattggag
     8341 acttaatatc tatcaaagag cgacctgcca tccgctccaa acgccgaaag ttcttcagca
     8401 catctctcgc attacatctt gtctgtttga aatctacctc tcgtaacaat tgcatcaagt
     8461 caaaccgctc ctttatgtga tataataaac ttgtctgttt tattatgtta gtcggagcga
     8521 tccggctttt ttatttgctc tcgattactt ccatgtcaac taatctcgct acagctaaat
     8581 tctctttgct tttcgctaac cacttgtcac attccatcgt gttttcaata cgaatgattg
     8641 ctgagttcga caataccttt tctacatatc cacgaaatgg atagataaat ccttctgctt
     8701 cacagcgaac catgtcaccg actttgaatt ttggtttctc acgtgtttta gggttctttg
     8761 tcggcatatc tagcatcaaa ccgccgatac catgactact agcgtaaaat ccgtctttta
     8821 gtttcattac tctaccccct catgatcgac agtgaccagt tcatatactt gcgttaatcc
     8881 accaagtcgt cgtgaaactt ggttagcttc ttcgagatta tcaaaccatc ttgcgctttg
     8941 gagtatgtct acaatggata atgtgttagc ccctatagaa cgttcatcgc ttctgtaaaa
     9001 ttgatttcca aactttacta cataaatctt cattctgtat cctcccattt aatatctagt
     9061 atcgaaattc caaacttacg aatagcatca cttgcatcag caacacactg acttgccact
     9121 ttatatgttt cttctgcaga aacaccatac tcccgttcga actttacttt cgctacattc
     9181 agttcttgat ttcttagttt tgctactctg cggtgtctgt tgttcatttt tcttcacctc
     9241 tgttttgtta tcaccatttc tttgttgaaa atccacaacg tctatactgt ttaattcgtt
     9301 tcttaggcct ttctagatca atcagaataa atctgtcgga tatatgataa tcttctatta
     9361 cagccgtata cattggattc ggaaccgagt accatgatct ttttttatca aactcatccg
     9421 catcatctct tgtaatttct atcatgttag cagacattag atagccctct tcttctgcct
     9481 cggatgggtg ggaatttgta tcccagcttc gaggaaacct ggccaaatct ctttttaaat
     9541 aaacttgaac attcattccg cttcctcctc atcgatctct atctcgacat ctctgccttc
     9601 taaaatctgt ttggtatcaa tgctttttgt tttcaacgcc aacccaccgt ctttttgtgc
     9661 agcaacaatg gtaatcgtca ttgcctttgt tccgtctggt atatggagca ttatctgtct
     9721 actcatcctt ccgccacctc ttccactggc acagcaaatg gccagtatct ttcatcaatt
     9781 gctttgatct gtttttcggt cagttggtag tggtgtgcaa ggtattgtgg caggtaagct
     9841 gttgatacta gtttaacttc attactgcaa ctgatctcct tgacaaggta ctcgccagca
     9901 attattacac tgtacaacgg ctctttctcg acctcgtagc cgttgtacaa gctcaatagt
     9961 gtttcatatg attgtctcgt tgcccaagcg agtaattctt ctccttgttc ttgcgtcact
    10021 atgccatctc gtacaaacca atcacagaaa taatatgaat caccatcttt agatttgatt
    10081 aaatacgcta ttttttcagc tttcgtaaaa gggtccgtag atttctcaag ccaaccggcc
    10141 acgagttgtg gaataactag cttcttcggt tcgtctattt ttttcattaa cgtaatacct
    10201 gcatttatac tctcgttata gcaagcagct agtgcatcat ttcctatcac tttaatactt
    10261 tctaactttt tgattgcttc ctgtttattc atcgctgttc ctcctaaaaa attaactgcc
    10321 aaagcaatat aatcactttc aatgatacaa atcccaccag tgcaataatt acagatataa
    10381 acgcacagtt tatgaaacag ccaactaagg cgcttgcttc ttctaaccag tctttcatta
    10441 ctgttcctcc ttcaaccata aatttggaaa atcatcggca tatatctcta ttgcgccaaa
    10501 taacttgatc gctgattttg attttccatg tatgtcagga ctgattaaac gatcataata
    10561 atcctgactg attgtttttc ccttgtactg ataacgtgta gccgcagtgt aatatactgc
    10621 tgtcttgctc agataaagat attttgctaa atcagccgat atccgtaaca aaatgattct
    10681 attcttactt ttaattccac aatttagcaa tgctgggaca ttcccatttt catcttttaa
    10741 agaatcgatg tagtcagtag ctaagataat cactctacct ccaataactc gctattctcg
    10801 tgtatatttc cggtgacttc atattcgtat cccatctttt ggtggctgaa tattctatat
    10861 tccattccac taattaatga tttacaaact aatccggaat gaaactcgga ttcttgtaca
    10921 accgtttttt cattaactaa atgatcaaaa ccattactta cattaaccag aactacatcc
    10981 ccttcaaata tttctacgcc attcttatct ttcagtcctg tggattgcat aagcacatat
    11041 ttatctggag ccatttcagc atgagtgatc aaccttcctg cttgcccata tttcatttct
    11101 tgtccaattg ttttaccttt aaatggtgtg taccacgatc gaaatctcgg tatcatttgc
    11161 tgccctccat gtattcgtct aatatctctt tatatttctc tacaaatttg aaacgatctt
    11221 gatgaagttt cttgctccaa tttgtttgcc gatccagctc acgcatttga tcgaaccctt
    11281 tttgaatttc gttgtaataa aattcaatgt ttgctgctgc cttccaatgc ctgctacttc
    11341 gcactcctgc ccctgtttca gccatttcca acttaactaa ttccgctcgt tcttttgatt
    11401 ttttgtcttt ctgaatcttc atcatgattt tcttgaggat gatatcactg tattgtgtaa
    11461 tgagattcat tatttctcct ccacatacct aaactgtctt cctttagaat caatatccat
    11521 attcttcgct ctatcccaga tgatgttttt gctcagacca gtaatttcag ataactgttc
    11581 agcggtacct gttactagaa ttcggtcacc gtgccagatt gcaatttttc tcggcattct
    11641 ccgtctggtt ttttcagtcc acattgattt accgagcttt tggacttctg caactatttc
    11701 tttgtcttct tgccaattct cagaatgtgt cagttcgatg attcgtttca ttgctgcttt
    11761 cttatccacg ctcattcctc caatctacga atttcccttc ttaagttctc tatgtgcaaa
    11821 tcgattgcct ttctcgccgt ttcattgacc atcactgcct ttgttcgttc cagatcgtca
    11881 atctcacgtt gaatgcttcg aattcgcatt tgaatcactt cttctgttgt catgatagac
    11941 cacctcttta aaaacgctct tccttgaacg tattccgata ttttttagct aatatcaacg
    12001 gcacttgata ttgatgacaa aacagctttg ccttgatctt aaagtctttt gtctgcattc
    12061 ctttaacatc tacgactttg acaagtttgc cgtttttata aaatgtgaag tcgggaatat
    12121 actcgatctt gcgatacttc tttccgtcta gttcaaattt cggcatcagc tcaaatcttt
    12181 cctgaagttt tactttccag ccattcgctt cagcttgcca caaggctaga tcgtaatact
    12241 ctgcttctgc gatagaatca aacttgatac ctcgatgaac agttttttta ttacggtatt
    12301 tattcatgcg atactacctt tcactggttt tatgcgcttg tctgctgttt gctggaattt
    12361 cagcgcataa ccttctgaat tcttaaatat cctagaaaca attctttcac cgtaggcttc
    12421 tcttagttca ggaccagata agtttgttgt gatgatcgtt gccttgttct gtcttgcttc
    12481 taagagcgtg tttaacgtgt tgtttgtaaa ctgcctacta tttgataccc cactacctaa
    12541 ttcagctcca atatcgtcaa aaaccaccaa atcagttgtt ttaatatcgg ctataagcga
    12601 tccttcaatt tcttttctca gttcagcatt gttataagaa aactttattt gctctaataa
    12661 ctcttgatag cttataaaaa gtattttctt gtcataattt gagcgctcaa gtatttccca
    12721 agctgtcgcc attgacaagt ggctttttcc gcttcctgat ttccctgata gaatgaaatg
    12781 tgcaggatgg ttcagtaaga catcatttac aaagctttta gctctttcta aagcaatttt
    12841 cgtttcttgg tccactacgt gataattctc cattttgcat ttaaacaaag ttttatctgt
    12901 taataccgaa ccattttgaa aaaaactcaa cgctcgtgct tttaagctgt cgttatatat
    12961 ccgttcggtc tgtatatcct ctttcacacg taacgcttta taaccacaac tcatgcatgt
    13021 tggtttacaa cgttctgaac catccttatt tttagctcgc caactataca aaggttcgct
    13081 acattctgga catttcccgc tttgcactaa tactcttctt attagtttct ccatagcatt
    13141 tgctaggctt tccatgtgat gcatctcctt tttaaattgg caagtcgtca tattcactag
    13201 gattgctgta ctgtagtttt tgactttgct ttttatgatt attcttgtct gctttgattt
    13261 cgaatttgag cttctcaaat ttttctctca atttcttagc acttctaata tttccaaacc
    13321 aaaattcatt tgtaggtagc caattgatca catactcaat cgcttctata gacgctttat
    13381 ctctttcttc catcaacctg attgtgtctg cccatttttc gatatctact ttgttcattt
    13441 cttttggaaa atcttcagtt aaattacttt gcaatttttt agcaaggcgt aagtgttcgt
    13501 cagaatactt acctttcttt tcttctttat ctatatctat atctttctct atctctatct
    13561 ctaactctgg tgtagttttg tctggacatt tgtccgacac ttgtcctcca gttattaaat
    13621 tccgttttgc ctcttctatt ttctttctgt attctctttt tctatctgct tcagttgagg
    13681 attttccaat gaaactttgt atatcagaca tataaattgc tccgttatct aatacgtcaa
    13741 taagctgcaa atcacggaaa atttgtaccg ctttttctac gactcctaca gaatgtcttg
    13801 taatagttgc gagcattgta gagttaaatg gaatcctgtc attaaacatc aacttacctt
    13861 cgtgttttag acttcttaaa taaagtttga gaagaatatt agaataaata tagccatctg
    13921 gcatactttc taagagaacc atctcgtcac tatcgaaaaa attctctttt agttttaaat
    13981 agtagtagcg tttgttgtca gacaatattt tttaccctcc tattctaagt ttcttaattg
    14041 tttcctggtt taacttgatc cctttgattt gatatttatt tttgaaattg atcacaccta
    14101 tattgtgttt ctccgtgtga tgaatcctgc agagtgctgc aaatgtgtac tctgaatgat
    14161 caacttcttt gcgctttcgt cttcctagcg ctttgtcaaa gtgatcgatg tcagctcctg
    14221 ttttgccaca gatgcagcag actctttttg tgatgcattt gtagaagtaa tattcttgat
    14281 tcgctggtaa aatctcatag ccttctttga aaggaatatg atgttcaaag atgaaatcta
    14341 agatgatatt tgctaagaca ttagcatcac tcacagttgt attcgattcg tctttgaggc
    14401 ttattttgcg ccctgtgacg ccttcaaaac ggaagtaaaa gaattccttc cagaagtccg
    14461 ttggcatgcc tgtatcgatg aaaatatcgc ctatcagcgc atagatgaag tttcgttgct
    14521 gcacagtaaa tcgacgtgga tcaataaatc gaacttcaat aatccgatcg ccatcatatc
    14581 cgtcatacat cgtctttagt cgatcaatgt tcacttcttc attgatcgtt gcacctatgt
    14641 cttttccttt gaactttttc agaaccgctg aatatgaatc gatcaatggt ttaaacactc
    14701 atatcacttc tcttttgttt cttctctgta ctgatcttca atccagttaa cgcctcgttt
    14761 tagaatgccc aagtctctct tggtccattt actgtcatca gcggttatag aagccgcatc
    14821 agtcaatgca acaattgctt catcaattga tttttcgtac ttgttagcaa ccagttgtaa
    14881 agcatccaag aatagctttt tgcttctttg agtagctggt tcaagcatcg agacatcttc
    14941 tggcatatct tcgccagcaa atatatatag ccctagccca aacattgcta gattttttac
    15001 aagacagcgc atgatcgttt tgttgatatc aaacatagtt gctgcttcaa ctcgcttttc
    15061 gatttttcca acaatctctt ttttcttcgt ttcgttattc cactgataat cattgacttc
    15121 gtaggtatat ggctcatctt tcattgcctt gtttgcacca tccatgactg gtaaccacat
    15181 gtcacgcttt actccgttga ctgtgatact ggtaaaaacc atatagcctg ttttcttatc
    15241 aaagacatac ggcagatatg tttctggatc atgatagatt tcataatcta ctgcctcaca
    15301 cactttgcta acttctgccc aagcccatgc ccacgaaaga taagtgaggt tatttcgttt
    15361 ttcagtaaca tctttcacat ttattttgta taaggtatta aaaagcgtat tatcacttct
    15421 atttttcaaa ggttgttcct tttcactcat caaattctgc ctccatttca gcaatgtatt
    15481 tcttacctga tccgtaataa gagatatcaa tcaagttatc tctgtcatac tcttctagcg
    15541 catcaatcaa gccatcttcg atgacataga tatattcagt ttttttggac ttcctcgata
    15601 aatggataag atagacatga tcccaaatac tcacaaaatt tcccaaatcg tcttgatcac
    15661 atgctagttc ttcatccgtc aaaagattac gtctgatttt tcgattgctt gtttccttga
    15721 tattcgattt gccccaacta ggatcagtca aatattgatc tagagtggaa agttcttttt
    15781 tcatgtggta acatctcctt agatgtattt tctttgtgac tcattgcttt ggtcggctga
    15841 gtcacttttt tatttgttgc catgcttttt gcttatcaat atgttgttgg cttaggatgt
    15901 ttggtttatt gtgtctccac cagcgattag caattattac gcctattttt agcgcttcag
    15961 ctctattcat cctctaacaa ctccatttgc ttaataattg tcatcgtcgc tgtagaaggc
    16021 atccaattgt taataaaatc gataacatca tcgaactgtt ttgccttgat acgatttcta
    16081 gctactgctc cagtgattcg cttaattccg ccattcaagt ctttaaacaa ttctgctttt
    16141 gctttgctat ttaaatgctg ctgattacaa ataactctaa ttttgttgcg aaccatatta
    16201 gaaattgttc ggtaatctgg ttcagctaga agttgattgt tcttcaaatc gtttaaatca
    16261 gactcgatag cgtctactct ttcattcgtt tcttcgtttg cagctagagc aagtaatgcc
    16321 atttctcgtt tagttgctgg aattgatggc aattggtctt ttaattcttt ttccatcttg
    16381 ttgaaggcct caatatattg aagtttaaaa cttaaagctt tcttaccagt gaagcccatt
    16441 gctaatagag agaagccgtc tctattcata taaataattc ggtatgattg tttattttga
    16501 ggatgaacat aagtatcttc ccaaaatagg tctgcccaat tttcggcaac cccctctttt
    16561 agttcatcaa tagccgtcaa aacatgtctg tgctttttat cgaagctttc tgcaacttgt
    16621 aagctcgtag tcacagcttc tttatttttc aaaattacta attcttgcat tatttcttct
    16681 ctcctttttg atataattct ggtggggtga aaacatgagc agaaaaattg ataatataat
    16741 attgttaaca actatttttt atttagtaat cggagcaact ctttccttac tgtcattttt
    16801 gtccaacagt agtttgattg tgttaaatca aaaaagcata ttaacagaat ttgcccacat
    16861 agattacttc tcttttttta aaactggact aatatgttta tttctatctt catttatcct
    16921 ctatatagga actacatttt gtctaatggt gcttaattta aaaatgttta taattgaaaa
    16981 atggatagtt attatcgcaa agattttctc atcgatatca attatcattc caatgacatt
    17041 gacgttttct aaagagcaat ttaacgtagc tactacattc gtatctttta tagctctatt
    17101 ttcgtttgct gttcctaaaa cttttcaaag caaatctagc aacgcaaagg ttaatgtaga
    17161 aaacactgat agaaaaaatg atgaaagtta ttaaaaaaaa taattctttc atttattgtc
    17221 aaacctttct tatagacatt agactttttt agtcagtccc tcccgactgg cttttttgtt
    17281 ttgtactcag cttcatccag ccctataaaa atccaaacca tgtacacaat cgtacctatc
    17341 aacgcttgtc tgcttcccca gaggcctaaa gcgtagatga ttagcggtgc gctgaatact
    17401 agtgctctgt tgaatttgcc cattaacttg cctccttcaa aacatctgaa ggcgataaac
    17461 tttttacacg atatttgttt gcgtctttcc actttaaaaa ccaaataaaa gtatggtaat
    17521 gaataaatgt ggttgagtga cctggacgaa gtattccttc tgcaaattca ggaattgttt
    17581 ccatttcctt gcagtattca actaaagtag tttttgacat gccgtggaac ttctccaaga
    17641 ttaaactttg tcggtaccag tcgtctggtt ttactgcctt ctcagcagct tctatcagct
    17701 ctgaaagtgt tggttttttc acttattatc cctcctaaaa attatcgttc catactcgat
    17761 gtttaacatt tcttgaagct ctgattttgt aagaatgatt ttatcttcca acgactttac
    17821 ctccctctac aattcgtaca tagtgataat cgaatctata attctgtttg cttctgcaga
    17881 agtcttttta ccgtttaaaa ttaaagataa gtagcttttg ctaattccaa atctttcagc
    17941 aagcatggtg taagttaaga actttgaact ttcgacatat tctttgattt tttctctatc
    18001 tcgttgagtg atttctgcaa tgtcagtcat actaaaactc ctttctaacc aatttcctct
    18061 aaatccattt gagggtaata gccctctgcg attaataagt tgtaaataaa cactcgccct
    18121 ttctgtgtcc atttggtatt cattacaact ttagtgccgc catcggcttt cggtatctca
    18181 cttgtgtgcg attttgtgta tccttggttc atatgttttc ggtaaagtat ccattgtccg
    18241 ctaactttat gctgaacacc taactcgttt agcaatttgt ttagagcaat tgccgacatt
    18301 ccgtaatcag ctgcaatttg agaagtagct accgtatctg tcgatgaaag aatcgtatct
    18361 aagtagctaa tctttggttc gtactcggca atttgttgtt cgagtaattg gttcttttct
    18421 tctaaatccg ctgctaaacg caatgcttgt gcaaaacttt gaggtacgtt tgaatagcta
    18481 cctgtttttc taattgttgg aaggacttca ctcgttaccc aacgtttaaa ttttttggca
    18541 gagggaagtt ttgattttaa gattaaactg taaaggcctg actcgttaat caaaacaact
    18601 gctgttccat tgacggtgaa cgaatcgttc tgcgtcttat cctccaagtc cacatgatct
    18661 cgaatagctt tttgcggatt cgaatatcct agaacgtctg ctacatcttt tcctacaaaa
    18721 tatggttcat catttactaa aatagttcga acttcgtttt gctcgaaatt aaaaatttgt
    18781 ggtgtattca tatttctcat tcctttcttt ggtataattt ccttatcagt gtgacaggct
    18841 gaaataattg ttaaggaggt gaaaatttat gaaaccagaa tttgtaatta ctatgcttga
    18901 tggagaaaaa atcaaaatat ttgccgatac tttggtcgtt ggatacgatg atgcgtcaaa
    18961 tatcgaagaa aaagatggaa tgttctattc ttctcaaatt tacgtgaata ccttgcaagg
    19021 tgatattggg gtatctactc ttgcaacatc aagaccgctt gtaggtctta tcggcttttt
    19081 gtcaaaagca acattctttt ctctcggcga tgatatagat tcggatgaaa ctgtcatcta
    19141 taaaacatca gctgttaaaa gtatcggttt caaatagtaa ttcttgctcg ttgatagcgg
    19201 aagccaactt ttcttccaac acatcggcga gctttctttt tgttggattc atttcaaagt
    19261 tgatagtctt gagaagcatt tttgcatcct taactttttg tccttgcaat tccttgatga
    19321 ttttatcggc ttggctatca attccatgtg ttagatcatc tatcattttt tgtaccttga
    19381 ttgtttggct catttccttg ttcattgttt agcctccttt aataaatgat tttttgtatt
    19441 ttttaatttc cataaattcc tcaaattgct ctttggtatt attaccacga ccatacatat
    19501 tgtgaaattc tttatggcaa ctgacacaaa gtgtaacgcc gtttgaaatc gctgcggatt
    19561 tagttgttgc atggctaaat cacgaatcac aagtatctac taaaggcgaa tcaatttcgc
    19621 caaaagaaat tgttcaaact tatttagata ttcattatgc tgttatctat ggtcagcttc
    19681 cagaagaccg taaaaacgat gactaaacgt gagctaaaat ctctgctatg gctgcaacca
    19741 tggcagagtc ttctttatct accgcgtgtt ccatcgccac ttttgcttgt ttcagtatgt
    19801 ctaatttcaa atcttcgatt tttgccgaga ctgttttttc catattcttc tctcctttct
    19861 tttatattcg taaacaaatt taacaacttt ttaaaaaact gcgttgacta aattaacata
    19921 cagtgttaaa atcagaacat agttaaataa gacatataaa cattgatttt aaaagctttc
    19981 ttggcggttg gcatttatta atcaatagtg ttttttgttg tctttttagt tgttaaactt
    20041 gtttacaaga atgatattaa caccttttgt taaaatagtc aacgaatttt aacaaataaa
    20101 gttaaatatt ttttcttgaa cgaaaggaat attgatatga taacgtttga gagaataaaa
    20161 gagttagcga aaaaacaggg aaaatcatta aataaagttg aagaagacct aggctatggt
    20221 aaaaacgtct tatatagact taagaactca aacccctcta cagaacgttt gcaagaaatt
    20281 gctgactatt tcgatgtctc tgtagactat cttctggggc gcgaagaaaa agaaacccct
    20341 aaacatgtgg atttatcaga aaacgatact gttttttctt ttgatggaaa agaaatatct
    20401 aaggagacaa tgcgtaaagc gattgcaatc gctaaagctt tagaggaaaa tgaatagttg
    20461 gagtgatggg ttgtatgtat ttgaaattga aagaaatgtt gagcgaatac aatttgaaag
    20521 tagtttacat ggaaatgaaa gagcctggct tctattatcc gaagcctaga atcatttttt
    20581 tgaatgaaaa tctatatgg
//
LOCUS       DAYYGO010000049        20417 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000049 DAYYGO010000000
VERSION     DAYYGO010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.050"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(387..1853)
                     /locus_tag="ACHA5T_001461"
     CDS             complement(387..1853)
                     /locus_tag="ACHA5T_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8887040.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(2144..4228)
                     /locus_tag="ACHA5T_001462"
     CDS             complement(2144..4228)
                     /locus_tag="ACHA5T_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8887041.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
     gene            complement(4753..5112)
                     /locus_tag="ACHA5T_001463"
     CDS             complement(4753..5112)
                     /locus_tag="ACHA5T_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFM8887042.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(5142..5483)
                     /locus_tag="ACHA5T_001464"
     CDS             complement(5142..5483)
                     /locus_tag="ACHA5T_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887043.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(5480..6154)
                     /locus_tag="ACHA5T_001465"
     CDS             complement(5480..6154)
                     /locus_tag="ACHA5T_001465"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM8887044.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(7330..8628)
                     /gene="asnS"
                     /locus_tag="ACHA5T_001466"
     CDS             complement(7330..8628)
                     /gene="asnS"
                     /locus_tag="ACHA5T_001466"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFM8887045.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(8668..9858)
                     /locus_tag="ACHA5T_001467"
     CDS             complement(8668..9858)
                     /locus_tag="ACHA5T_001467"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM8887046.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(9879..10406)
                     /locus_tag="ACHA5T_001468"
     CDS             complement(9879..10406)
                     /locus_tag="ACHA5T_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFM8887047.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(10453..13242)
                     /locus_tag="ACHA5T_001469"
     CDS             complement(10453..13242)
                     /locus_tag="ACHA5T_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFM8887048.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(13389..13589)
                     /locus_tag="ACHA5T_001470"
     CDS             complement(13389..13589)
                     /locus_tag="ACHA5T_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8887049.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(14042..14362)
                     /locus_tag="ACHA5T_001471"
     CDS             complement(14042..14362)
                     /locus_tag="ACHA5T_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887050.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(14865..15983)
                     /locus_tag="ACHA5T_001472"
     CDS             complement(14865..15983)
                     /locus_tag="ACHA5T_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887051.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            16216..16338
                     /locus_tag="ACHA5T_001473"
     CDS             16216..16338
                     /locus_tag="ACHA5T_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887052.1"
                     /translation="MHLIMIFSLLSIISLFAFSASRLLDAETQPIPVKVRKNDR"
     gene            16454..17761
                     /locus_tag="ACHA5T_001474"
     CDS             16454..17761
                     /locus_tag="ACHA5T_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFM8887053.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            17882..19081
                     /locus_tag="ACHA5T_001475"
     CDS             17882..19081
                     /locus_tag="ACHA5T_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFM8887054.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(19108..20376)
                     /locus_tag="ACHA5T_001476"
     CDS             complement(19108..20376)
                     /locus_tag="ACHA5T_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFM8887055.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVRKGILFLPIVKRMKEKNKV"
BASE COUNT         5911 a         4346 c         3562 g         6598 t
ORIGIN      
        1 aataaatggt gttcaatcag ctgaccttca gcattagctc aaaaagcggc aaaatatgag
       61 gagctatttt tccgtttgtt tctctaatac tcaggtcaaa tcgctgattt tacaacctct
      121 tccttaaacg gtgttccaaa gcttgcatct gcggtaataa gcccagccaa acgaaaaata
      181 tgaggggcta tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac
      241 ctctgattca cggtgttcca gaagtagctt cttcgaaaaa caagccgaaa cttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttgt aacaagtatt tctattttaa ggctgaacca ctgtttctgg ttcagtgatt
      421 tctttgtttt tgtctcgacg agcaattgct ggcaagatca tacctaataa gatcaagacg
      481 attggtgtga tgatattcgt aaataattgg aaccaccatg ctttaggatc agcagtataa
      541 tctacttttg gcaccattcc taggatacat gcaaacgcag tgaaggcaaa gcaccaagca
      601 ccaaagataa aaccgatttt tggattttta acaaatttat attcagaatt gaatttctta
      661 taagctttat tcaacatcat atatgccaag aacacccata ggtaacgcat tggcatgaca
      721 actgagttca agttcgtcaa ccattttact aattcattca tatctttgat accaaaaatc
      781 ggcaacaaaa taatgattcc tactagtata cctgtcagaa gataaccatt caccaatgta
      841 cctttacgat tacgtttacg tagccagttt ggtacaaatt ctggatccgc atctgctaac
      901 aagattttta gtggtgcatc aattgaaaag gctaatgcgg agatttgtcc aatcatgttt
      961 gtcaatgcgt aaatgatcat caatgagtta ccgataccat agtaattacc taaaatttga
     1021 aaagcactgt atgccccatt tgccatcaag tctttaggaa tattatcact agcaaacagc
     1081 atacccattg cgactgagcc taatacagca caaatcccta ccattgctgc taagaagatc
     1141 atccctttag gaaattcctt tgcaggattt cttgtagagt tgacataagg ggatattttt
     1201 tccgcccctc caactgcaaa tactagcatc gaaacggttg tgaaataact gaaatcaaat
     1261 gttggaatat atgttttcac ttgattcata tgtggtgtgg caaattccat cgttggattg
     1321 atgaacggtg ccgttacagc taaaagaata aataagatcg acatgacgaa catcgctgtt
     1381 ccggccagtc cgccaatcac tttcaaagta gaaagtcctt ttgttgataa atataaaaag
     1441 gcaagaaata taataagtga aataactgct accatttgta ctgacatcgt atttacgata
     1501 tttccatttc cttggactgc ccaaccacca gcaatcagaa ttgcttgagg cttttgtgcc
     1561 aaataaggaa tatggacgac ccagtaagtc caagcggcat agtatgccag tcgtttatta
     1621 gatgtcgtat tttcgaccca tgaactgacg cctcctttac tatccttgaa ggctgatcct
     1681 aattgtccaa cgatcaatgc gtatggaata aaataaatgg ccaaaatcaa tagccaagag
     1741 gtgacaactg atactccttg ctgagcatag ttattaacta cattccccat gccccaaacc
     1801 atgttaaatg caattaacgc aacagtaaac cagcgtaatt gtttgttgtt cattcgttct
     1861 ctcctttatg ataaaaattt gttatccaga tgtaatcata ttcaattttt aacaaactta
     1921 caagacagtg ttattttatg tgtgtattct tttatttaat acacatcgtt ttttcatatt
     1981 ttttccatca aaaaaataag ttattttctt ttatttcatt gaccttattg aatttcaagc
     2041 tattttgaat tttctgcaaa attaaaaaca ttagttattt tataatccta tctattttct
     2101 ttacattttt cattaagaaa aggatataaa aaaaagagga cgttcattcg tcctcttttt
     2161 ttacatgcaa gcttcgcatg gcattcaaaa cagctaatac tgtcacccca acatctgcaa
     2221 atactgcatc tcccattgat gcaaaaccaa aagcacctaa ggcaagtacc aaaattttta
     2281 tcccaatagc aaagacaatg ttttgtttga cgattttcaa tgttttcttt gctaaatgga
     2341 ttgcttctgc aatcttcgat ggttgatcat tcatgatgac tacatctgct gcttcaattg
     2401 cagcatcgct tcccaatcca cccattgcga ttcctacatc tgctcttgct agcaccggtg
     2461 catcgttgat tccatctccg acaaatgcgg tctttttatt gtttctttgt aagatttcct
     2521 ctaaccgctg cactttatct tcaggaagca attcgctata tatcttgtcc acgcctactt
     2581 gcttcccgat atgatcagca atttttttcg agtcgcccgt caacatgact gtgttcttca
     2641 caccagcctg ttttaattga accaatgcgt ctacggcatc ttctttcaag gtatctgcaa
     2701 taacaagata accactgtaa ctttgatcga tcgctagata aagcaatgta ccgatttctt
     2761 gacttaaagg aaaagagatt tcaaatcttt ccatcaattt gtgattacct gctaatactt
     2821 gtttgccatc aacttcagct aagatccctt cacctgcaaa ttcctggata ttagttgttg
     2881 gtaaaagcgg acctgcgtag ccttctaaaa cagattgagc aatcggatgt gtagaaaact
     2941 gttctgcact cgcaatatat tgcatgaatt tctttggatc gatttttgta tcgatcgtct
     3001 ggatcgaaaa gtcacctttc gttaatgtgc cggttttatc gaataccact gtctcggttt
     3061 gcgcaagcaa ctccaagtaa tttgaacctt ttactaatac accgatttta cttgcgcctc
     3121 cgataccgcc aaagaaacta agtggcacgg aaatcactaa tgcacatgga catgagatca
     3181 ctaaaaatgt caatgctcga tagatccatt cagaaaacgc cgctccagaa acaacaagtg
     3241 gaggtaaaac agctaataaa acggccaatc ctacaacgac tggtgtgtaa tagcgcgcga
     3301 atttagtaat gaaattttca gctggtgctt ttttactgct ggcattttct actaattcta
     3361 ataatttgct gatagtagaa ttttcataag atttttctac ttgaactaaa aatggtttat
     3421 ttttattgat aaatccgctt aatactgttt caccaacata tacagattta gggactgatt
     3481 ctcctgtaag tgctgacgta tcgactaatg attctccttc gatgatcgtt ccatctaacg
     3541 gtacacgttc accaggcttc acaagtacaa actgaccgat ctgcacgtct tcagggttca
     3601 cggtcatcaa tccttccgtc gtctgcacgt gagctgtttc tggacggatc gccatcaatt
     3661 cgcgaatcga ttttcttgaa tgactgactg ccaatccttg gaaatactcg ccgatttgat
     3721 aaaatagcat cactgctacg gcttctggat attcaccgat caacatagcg ccaatcgtag
     3781 caacagacat aagaaaattc tcatcaaaga tttttccttt gacgatattc atcactgcct
     3841 tcttcactac atcatatcct atcagcagat aaatcaataa ataccctatc aagcgaattg
     3901 gttcattaaa atcaaataat agcaataaag cgatacctgc aagggaaaat aaaatccgga
     3961 acagcgtatt tctctctttt tcatttccat gttcagatac tttttcctta tctatttctg
     4021 ttactttgac atctggttct aattccttca ctactttttt tgtttcttgt tcaatcgtag
     4081 ccaaatgatc ttcctctgcg tcaatcgtta gttttccaga agtgaagttt accgttgctt
     4141 ccttgactcc attgatcttc tgaactcctt tttcgatttt cattgcacag ttcgcacaat
     4201 ctaaaccttc taatctgtaa cttttcattt taatgacctc ctcgcctcaa catatgaaca
     4261 tctcttcata actaattata aatgaatggt tattcatatg tcaaggtatc cgaaagaaaa
     4321 aatgaaaaac atctgcatcg gaaaatttct tccatacaga tgttttggct tattctttct
     4381 ttttctcttc gatcattgcc cctgctctta tccaacccgc acttgccttc ttgatcttga
     4441 caggatagca actaagtttg aagcctgttg ctggcaattt gtctaaattc attaattttt
     4501 cgataggaca atatcttttt tcaatccctg caaaatgtcc ttcccagata ttcgaagctc
     4561 cactacgctt tcaaaaatat agaaagagtt ctttaatgaa aattatttca actttccata
     4621 aacagtaatc ccatataaat aaaagacgta aaagtcacat gggagaactt atacaggaaa
     4681 acacatagag tcaggtgaaa tacatgtatc ccacctgact ctatgcgttt tctaaccttt
     4741 attattctct ttttatacaa tttctgcacc gaatggcatc aacgccaatc gagcaagttt
     4801 gaaagattgc ttggcaaaag gtatccctat gatcgtgata catagaagga tgccgctgac
     4861 gacatgtgcg agagcaattg gtaggcctga aacgatcagc cagatgatat taacgatcaa
     4921 tgacatgcca gactggtgat aaattacttc tttgccaaaa ggcgcaaaag aaagggaggc
     4981 gatcttaaaa cattgcatac cgattgggat acctatgatc gtgatacacc aaagtatccc
     5041 cgctgctaac cagctaagac caccgattag tccgccgaat ataaaccaga tgacatttcc
     5101 taaacaactc attgtatttc tccttctatt actgtttttg attaaaactg ggcaaaaatt
     5161 gattttattc ttcttttttt agcttccagc atcaagtatt ctgcaaattt tttttggatt
     5221 tcttccgtat tttcttttgt tactaatgtg tgttggatta gttttcctga tggtcgctga
     5281 tccacccgtt cttctgactg ttgcacgtcc gcttgcgcaa atccgataat ccgtttttct
     5341 tttgttacag caacgagcaa tagtttattc ttatcattta ggtcagctgc cgtttttgct
     5401 tttacgaatg attctggatg atcagtaatc agctgattga tttgaggtaa atcttctttt
     5461 actgcccacc gtatttccat catttctcac ttttctttcg ctcattttta tctccactaa
     5521 aaaaatcgac tgcggcaatt gcatctatcc agctttgtgc aatgatctca tgtccgccaa
     5581 tcgttgggtg gaccccatct tctcttgtat atttcgaaaa accataagca atccctaaag
     5641 cattgaatat gccatctaaa ggaaccagca atgcgccata ttccttagca agtttacgaa
     5701 caatttggat tttagggtct aagtctttac gccagccatg acggtcgtcg gttataggaa
     5761 gtacaaacgg ctccatcaag atgatgttgc agatttccgc tgtttgaagt tcatttagta
     5821 attttcgata aacggattca aattgctgcg cagatttctc agttccgaat atagcctgat
     5881 cgctagtatt atgccaggta tcattgattc cgatcaaaat cgatacatag tcaggttgta
     5941 aagctaaaca atcttcttgc cagcgtactt gcagatctgc aattttgtct cctcctatcc
     6001 cacggttcat gcattcaaat ttcatcgttg gatattggga gattagtttt ccggcaatca
     6061 aaagtggata acctttacct agatcagttg cttctgctcg gtctcttcct gcgtctgtaa
     6121 tgctgtcacc gataaataat atacgttttt tcatttgtca aaaactcctt tttgaataaa
     6181 acgatgattt tcttccttat attatcccat aagccctatc aagacgaaag agaaaatata
     6241 tgtataaaaa atgacaccag cctagtccct tcaatcaatt gaatgaacgc tcatcaagtg
     6301 tctagttttt tatacttttt ttcttaacgt gccggattcc ccttgattcc ttcgttgttt
     6361 ggtccagaga aatcgatcac acctaagctt ccgtcaatca gaccactgat gacgccatca
     6421 gaacatatat ttataggctc atctaagact tccagtaaat gttcttttac taatatctct
     6481 tctagattgc ttgggataga ttggtcataa ctttatctgg tccgatgatt tcaaggatga
     6541 attaaactta catatggatc attgcatgct gaaaatacgg acctactcca tcttcgaatg
     6601 ttactattag tttgttttca ggtactagat gctgctttat ttcaacagca gaagcatcat
     6661 caaatcagat ttccatttga ataaccttct ttgtatatgt ttgattgatt gcgatttcgt
     6721 tatagaacct tgtgaatcaa atagcaattt cttttgcttt cttctcttgt ctgctggaaa
     6781 cttcctttat cccgtatttt aaaaaaagaa ctgcgagagt tcgcagttct tttttttaaa
     6841 taaacgttgc tctccggcca actcctcggt cacaagtcac aatagtcatc aaacatgaaa
     6901 aactgttttt tgaaattact ccttgtgact cggagctgaa cggcaggcgc acaacctctt
     6961 gatataaacg gtgttcaatc agctgaccct cgatattagc taaaaaaacg gtaaaatatg
     7021 agaagctatt tttccgtttt gtttctctaa tactcaggtc aaggcactga tttcacaacc
     7081 tcttccttaa acggtgttca aaagccagtt tctcggtatt aagccaaatt tatgcaaaaa
     7141 tgtggaaagc catttccaca aaatttttct taatactcga aactaaacag ctttttcaca
     7201 acctctccac taaacggtat tcaaaagctt acatctgcgg taataagtcc cgccaattca
     7261 aaaacatgaa cagctgtttt tgcttggctg ttctaattac ttgatgcaga acagcttttt
     7321 catcacctct tatggataaa ttcggttcaa caaacgtggg aatggaatag cttcacgtac
     7381 atggtcgata ccagcaatcc aagtgactgt tcgttcaagg cctagaccaa aaccagagtg
     7441 cggaacagaa ccgtatttac gtagatcaag ataccatgaa tattcttttt catccaaacc
     7501 gtgattacgg atttgttcta gcaagaaatc atagtctgtt gcccgttcgc ttccgccaat
     7561 aatttcaccg taaccttctg gtgcgatcat atctgcacaa attaccacgt cttctcttgt
     7621 tggatgtggt ttcatgtaga atggcttgat ggcttttgga tagtttagga taaagacagg
     7681 ctgttcgaat gaattcgcaa tgaacgtttc atgaggtgaa ccaaagtctt cgccccattc
     7741 gatgtcatca aatccatttt tcttcagtaa ttcgattgca tcatcgtaag taatacgagg
     7801 gaatggcaat tttgtataag tttttaagac ttctttgtct cgtcctagta catctaatgc
     7861 atattcgcaa tgatctaaaa cattttgtac taagaatgct acatattgtt cctgtacttc
     7921 taaactttct tcttgatgag tgaaagccat ttctggttcg atcatccaga attcgatcaa
     7981 atgacgtcgt gtttttgatt tttcagcacg gaaagtcgga ccgaatgaaa agacttttcc
     8041 gaaagccata gcagcagctt ccatgtataa ttggccactt tgagataaat aagctttttg
     8101 atcaaagtaa tttgtttcga atagatccgt agtgccttct ggtgcagaac cagtcaagat
     8161 cggcggatcg attttgacaa aatgatgttg attaaagaat tcatacgttg cacgaatgat
     8221 ttcatttctg atctgcataa tcgcatgttg tcttgaagaa cgcagccata aatgacgatg
     8281 atccatcaag aaatctgttc cgtgttcttt tggtgtgatc ggataatcgt ggctctcgct
     8341 taccacttcc acatttgtaa ttccgatttc ataaccaaat tttgaacgac tgtcttcacg
     8401 gatttctcca gttactaaca cggatgtttc ttggtgtaga tttttagctg tttggaaaac
     8461 ttcttccgac acttcgcttt ttacaactac accttggaaa aaggctgttc cgtctcttaa
     8521 ttgtaaaaat gcgatttttc cgcttgatcg cttatttgcg acccaagcgc cgattgttac
     8581 tgtttctccc acatggtttt tcgaatcgat gatttgaatt tgttccacgt atgactctcc
     8641 ttttatattc cttgaatttc ttcaggatta attttgactt ttactctcaa taaattgctt
     8701 gattctgcgt accgcttctt ctaacgtagc gcgatcagtg gcataactca tgcggacatt
     8761 ttctggtgcc ccaaagcctt cgccagtcac aagcgctacc cctgtttctt ctaaaagatc
     8821 ttctacccat tgtgtgacat tttcatactt acacatgcgt agcgtttctt ttacattagg
     8881 aaaaagatag aaagctcctt gcggcttttc aagagaaaca cccggcagtt ctgccacaag
     8941 cggatataac gtattcaggc gttcttcaaa ggcgttccgc atttcttcta ctgtatcttg
     9001 ctcacctttc aatgcttcaa cagctgcata ttgactgact gctgtcggat tgctagttga
     9061 ttgggaagca acagcgatca ttccattgat gatcacagga tttcccaccg cataaccaat
     9121 gcgccagcct gtcatcgcat atgttttaga cacgccgttg atgatgatgg tctgattttt
     9181 gattgcttct gaaatcgttg caattggagt aaactcattg ccattataaa ctaaacggcc
     9241 ataaatatca tcagcaacta tcaaaatatc attttttact gcccattcac cgattgcctg
     9301 cagttcattc ttgctataaa tcattccagt cggattcgat ggagaattga tgattacggc
     9361 ttttgttttt tctgaacgtg cttgttctag ctgttcaaca gtaactttcc agctattgga
     9421 ttcttcccct ttcacaaaga caggaagtcc ttctgccagt tttacttgct caccgtaact
     9481 gacccagtaa ggcacaggaa tgatgacttc atcttgcggg tcaaggatcg tttggaaaag
     9541 ggtatacaaa gcaaatttcg ctccgtctgt tacgactgtt tgtgaaggtt catagacaag
     9601 tccatagttt ttcttcagat aatcaacgat tgcctgtcga agctctggga ttcctgctgt
     9661 tggtgtataa tagctagctt tcccattgcg gattgcttcg attgctgctt cttggatgtt
     9721 ttctggtgtc gcaaaatccg gttctcccac agtcaaactc agcacatctc tgccttttgc
     9781 ttttaatgcc ttggcctttg ccgcagctgc caaagtgaca gacggttcta atcgttttgt
     9841 tctttttgat aactccatcc cattcattcc tttacctttt aaattccctt gattgtcttg
     9901 acttcatctc cagattcaaa gccaatcagg taataattca gtttcccatt tgtttctttt
     9961 cctactactt cccacacagg ctgatcatca tacatcccta aagcagcttt ttcgatttgg
    10021 atttcaggat gggccgcagc aattgtctgt tttgcttcgt tttcagtcag tccctttgtt
    10081 tgatccaata cttttacttt ttcaccagat ttaggaatga taacggcgat ttcttttcct
    10141 ttatcatttg tacctgtcag tgaaaagtat gtcttctttc gagtaaacca ataaaaatga
    10201 tcggtttcct caagatttgc atatttcttt gcgatttcta ctgcttcttt cttcgcctgc
    10261 accgccggac gactggaacg caagtaaaaa atcgagctga tcacgatgac cgctaataaa
    10321 aatacgatca agccaagtaa cacgcgatta atcgtttttt cctttgtctg ttttttcttc
    10381 aagggtcgtc gctcctttat cttcattcct tttcattata gcagatttta attatgaaaa
    10441 aagacagctc ctctaacttc cttcttcatt tttctttaag aattcatgga tatcctgata
    10501 gatttcctcc atagctagct ctttgatcgg tacttttttc ggtaatgcat ggatgatccg
    10561 tgcgccatat tttgcagtga ctagtcttcg atccaaaata agcaatacgc cacgatctgt
    10621 ctccgtcctt atcaagcgac ctaatgcttg gcgtaacttc aaagctgctt taggtacgga
    10681 ttcctggtaa aaaggattga tcccctctga ttctaaataa gcgttcctag ctttgaccat
    10741 cgggcgaagc ggattatcga agggtaaacg tgtgactaca acgatctgca aagcatctcc
    10801 aggcaaatct acaccttccc aaaagctgtc agcgccaaac aatacgcttt tttctgacaa
    10861 aatgaagcgt ttcaaaagct tctcccggct tccaccaaac ccttgagcta gtatttctcg
    10921 tcctttttct agcatagaca gatgcatttt ctgatagacc ttctgcaaaa catcatgaga
    10981 agtgaataac acaagtattg gccgatcttc ttgttcgatc agacctgtaa gtgctgtctc
    11041 taaataatcg atatatgctt gattgctgac ttgatggacg gcaggtgcct cttttggcac
    11101 atagatcctt gcctgtttct cataatcata aggagaggca atcactttaa gaggtgctgc
    11161 ttcaatgcca agcctcttag agagatagtg tcgattaggg ccgatcttca gcgttcctcc
    11221 aatataaaga atacgatcat atcgaggata ccaaacagtc gacggcaaca tagccgcttg
    11281 aaaatcatgg agatgaaaaa tcatctgctg cgctgtgttc ccaggaacga accaatggat
    11341 caaatgatct cgccagtctt ccagccattg atccatcagc aagtattgct tgtctaattg
    11401 atcaaatagg ctatataaac ttgctaaatc gatccgcatc tggtttgtcc agctttcttg
    11461 gataatctca aaagtcagac gcatttgtga ctgtagctgc tggatctcct gataaaaaag
    11521 ctgcaggtga tcaatggctg tcccagattc cagtggcagc tgatccaaca tctcttttgt
    11581 catcatgact tcttctggat actgtccgtt aggttgcttg ccgctcacta aactcattaa
    11641 tccttctgcc atacaaatct gtgcttcgca tagcgcagaa agttcttctc gataaatcga
    11701 taaccagcgt aaggcttcat gttctttcgg taaatagctt tgtattcggt caaacagctg
    11761 atttggttct tctaattgct ggatttgttt atggaaatag ttaatactta atcgtttgtc
    11821 atttactttt tcagcgattt ccggcaaatg atgtacttca tcgatcaaca aaaatggact
    11881 agctggaagc aatggttctt ttcgctgtgt ttcttgtact aaaaacgcat gattgacaat
    11941 caaaaaatta ctttgcctga ttttcttttg caagaaacga acaaagtctt gttcgtaaaa
    12001 tgactgattt cttgctaaaa atccaatacc tctgtgcgcg atatctgcaa agaatgaatg
    12061 gttcaaattt gttaaatgta attcatccaa atcaccagtc ttcgtttgcg tcagccaaac
    12121 taagaccgcc atttgataca aagcatattg tttttgttct ggcggttcca caagtgtgcc
    12181 tttaaaacgc tgcaaatcaa tatagtggcg atagcttttg ataattacag gttgtatggg
    12241 ctgatctagc aattgattca gcaatggaat gtctttttct atgatctgct gctgcaaaac
    12301 tagcgaaacg gtactgatga cagcgggttt ttcaggtgta gccaaataac tcaatggcag
    12361 cagataaccg attgttttac ccattcctgt agctgcttcg atcatcaaat cttttgattc
    12421 atcttttgtg aaatggtcgt agaccgcatt catcagtctg ttctgttctt ttcgatacat
    12481 cagtgtttta ccaaacattt tttcttttgc ttgttttttt cttggatatg tccgctcgcc
    12541 ataatggacg gatgtgaata gttccacttc ttttttctgc aaagctagcc catcgacgat
    12601 ttctaatgag ggatcaagtg gctctggttt ttctttcatt tcttcaatca catgataaat
    12661 gaaacgactc gtatccatac ctgtgtgtct acttaatcgc gcaatctgct ctaacgtgat
    12721 gattggcaat tttctcatcc gttcttcgat cagcaaaaga agttgacctg tcacttcggc
    12781 atcactatct gcctgatgag gattctcatg aataaatcca aggctttctg acaaatcagc
    12841 taagcgaaag cttggttccg tcggcaagaa aatttgtgca agttctactg tatcgatgcc
    12901 tggtatcttc aaactaggaa gtccgcatcg catgagttca tggttcaaaa aattataatc
    12961 aaaataaata ttatgcgcga caaatatggt gtctgccaaa agattgtaaa tcgtttgggc
    13021 gacatcttcg aaataaggcg ctttttggac gcgttgattc gtgatccctg ttaaatgctg
    13081 gatttgtttt gaaatcttcc gccctggatg aatatcgatc gaaaaacgtg aagtgatccg
    13141 acctgattca atcatgacgc agccaaattg gatgatccga tcttcttttg gattcgtccc
    13201 agtcgtttcg atatcaacga ctgcataagt tcttggtttc aaaatatctt cctcttctcc
    13261 tgcctctaat gttcttttga aattatacta caaagtgcag aaaaatactc tcatcgcttt
    13321 ttaaagaaaa tagacaaaaa cgcccagaca cgcaaatgtc tgggcgttta ttttttagta
    13381 taggttaatt aagctttagt aacgtttgtt gcttgaggtc cgcgttggcc ttcttcaaca
    13441 tcgaaagtta ctgcttggcc ttcttctaaa gttttgaatc cgtcgccttg gatagctgag
    13501 aaatgaacga atacatcgtt tccgttttct gcagtgatga atccaaatcc tttttctgag
    13561 ttaaaccatt ttactgtacc tgtttccata ataaaaatcc tccttgtgta tcaacacata
    13621 ttttgcaatt acttgaatgg tgtatatctg gaagatgttt atttgaaaca agctaccaca
    13681 gattaccgaa caaaaactac tcctatagta tatcataccg tttacaaaaa agctagagtt
    13741 tttcgacttt ttttattaaa aaaagttttc tcagcgatta cataaagtga aaaaccgaac
    13801 ttttttttaa aataaacgac atattgttca gttggtgttt ttttgctctg atgttctttt
    13861 ttaaactaaa aaaattattc tttctgacta cttatcggtt ctgtttcaac ggtcgaggct
    13921 ggggaagatt ctttagtagg tctgatgttc tcatggtatt tctgatagcc tatgacagtt
    13981 accagaagaa caaggcaaac caatacgacc aacgagatta gtttttcttt tttgctcata
    14041 tctatacctc ataaataaca ggatgatgtc catcatattt ctgagtatga gtaaaagcaa
    14101 aagctgcagc tgtagaatgt aagaaatgcc agcaactttc ataatcacag gtcggacagt
    14161 accaaaagta accaaatctt ggctgcaaag accgttcttc tgtttcagga atcgcagaat
    14221 caagatctgg tgtctgtaca taaacatttt tgtacagacg tgtgcctgta ataggaaacc
    14281 gctgtgtttc ttcagcaaaa actggctgac ttgttaaact tagggataga gacagcaaac
    14341 accataacag tctttttttc ataaagacct ccttatcgat aagtttttcc caattaaaat
    14401 catatctcaa gaacgcccgt tattcacatt ttgttcataa atgatgtttt ttgtatcata
    14461 atcgtcaaaa agaggtcgtg accacgtcac aacctctttt ttaaacggtg ttccagaagt
    14521 agcttcttcg gaaataagcc gaaatttcac aaaaatttga gaaataattt ttgtcaatct
    14581 cttcttattt cttgaagctg accacttctg tcacaacctc ttccttaaac ggtgttcaaa
    14641 agcttgcatc tgcggtaata agcccaccaa acaaaaaata tgaagagcta tttttgcttg
    14701 ctgttcttat tacttgatgc agaacagctt tttcacaacc tctcatacac ggtgtttaat
    14761 cagctgacct tcggtattag ctcaaaaaac ggcaaaatat gtgaaactat ttttccgttt
    14821 gtttctctaa tactcaggtc aaaacactga tttcacaacc tcttttaata acgaagaatc
    14881 gctgtcagac tcttttcatc tggcgcatct ttttgatcca aaataaacac ttttccgcca
    14941 ttaatgatca catcatctgc taatgcattc aacacttgac gccgatcata ttctgtttca
    15001 ggatcttcac cgaaaccatc agctaagttt gacgtagcga taaataaatg tgacactttt
    15061 ccttctttcg cagctggaac gatatcaact aactgatcga tgaatttctt atctaagagt
    15121 ttattgtatt cttttgattc ttgagctgcg agttcacttg acagcttctt cgccccttct
    15181 tcgatatctc taaaggatag ctgcgcagga gaagcagaaa tcgcaacgtc tgcacaataa
    15241 tagggatttt tagcaatttt tttgaacatc gtctgatttt ctggtagagc aaataaataa
    15301 atcggccatt gttctggatt ttcgaaagaa tccttcaagt acgtatcaac tgcttggtaa
    15361 tagttgaccc aatcgatctc tacttcttca tcttttggat ttacgccatg gaaagttact
    15421 ccatctcggg ctccgcctcc tccattagaa gagaagttca aaccgccacc tgtcagttca
    15481 gatcccaatg cagtaacgac atcaaccggt gccccttctg gtagagatac ttcttttact
    15541 tgtttgttgc tgacttcata caatttcatg gagtcgcggt tcaatcccaa taaataataa
    15601 gaatagttga actgcccatt tttgataata gccagtaaat aaggaagatc acttacatag
    15661 tattgatcgt ctaccgggat actcaaacga tggatatagg tttggtcagc agttataatg
    15721 accgctacac tcgtagttgc actacgccaa aacgaaggat ctcctaacaa attttcaatt
    15781 ttttcttgga acggggacca gtcatgttcg gaatattttt tctcaaaacg tttctttgct
    15841 tctttcgcaa aatttttgaa tttgatctga tctttttcca catcttgatg ggcgacatgc
    15901 gtattcagca tgatcgtgac gaatggtcct ttagcgcctt ctgaagtcaa ttgtgacaat
    15961 aattctttat ccttttttgc cataaacgta cccctcctaa aaaaatcatt cctttgtttc
    16021 tatctaaagt ctaccacaac actatttttc catacaataa aacgtatcta aaaaaataaa
    16081 acggtttcta cggttttaaa tttagctatt tttttaagtt atcttcataa tttcctcaat
    16141 tatttagccc cagtcttcaa ctattcttca tgaaatgctg ataaactaag aaaaagaata
    16201 aaagaggtgg atcttatgca tttgattatg attttcagtt tattaagtat tatcagttta
    16261 tttgctttca gcgcttcccg cttattagat gcggaaacac agcctattcc tgtaaaagta
    16321 cgtaaaaacg accgctaacc ttccataacg tgcgttctta tcctaaagtt ttttcagaaa
    16381 cattgaaact ctctcccctc tctgataaaa ttttaatttg tagcgaaaca aattaattac
    16441 aaggagatcg gatatgtcca taaaaaaaac acgtctagtc ctattattgg ctagtctcat
    16501 tctttgcctt gcttcttttc taccagctgt tgctgtgcaa gctgaagata ctttcaaagt
    16561 aaatgcaaaa gcagcctttg ctgtagatgc gcaaagcggt aagattcttt atgatcaaga
    16621 tggagaaaaa accatgggga tcgcttcaat cacaaaaatc atcggtcttt acatcgtcct
    16681 tgatcaagta aaagaaggga aactatcatg ggatgataaa gtttcaattt cagattatgc
    16741 agaaaatttg agtgtcaccc cagatttatc aaatgttcct ttgcataaag aaaacaccta
    16801 caccgttaaa gaattattcg actctgcaat catccagtca gccaacgctt caatggttgc
    16861 cttggcagaa aaaatctctg gcagcgaagc aaaattcact gaacgaatga aagaacaatt
    16921 gaaagattgg ggtatcaaag atgcaacgat cgttaatgca tcaggactga ataactctta
    16981 tcttggcgaa aaccgaccag aaggtactgg agaaaacgac gaaaatcaaa tgtctgctca
    17041 agatgtggct atcgttgccc gtcatttgat cttggatttt ccagaaatac tggatgtatc
    17101 tagcactacg acacaaatgt ttggcgaaaa cacgcaatca ccagtagaaa tggtcaactg
    17161 gaactggatg ctcccaggat tcgtgaatta taaagaaggc gtggacggat taaaaactgg
    17221 tacgactgaa cttgcaggag cctgtttcgt cggtacgatc actaaaaacg atcaacgaat
    17281 catcactgtc gtattgaatg cagacggcca tgcggaaaac ccaagtgccc gtttcaatga
    17341 aacagctcgc ttaatggatt attgctacga caattggtca gaaaaagagc ttggaaaagc
    17401 caatgcaagt atccctagtc taaaaaccat tgatgtaaaa gacggaaaag aatcaacagt
    17461 gcccgttgtg cttaaaagtc ctgtaaaagt atgggtacgg aacgatatgg atactggaaa
    17521 attaacaatc acaccaactc ttgacaaaaa acaagtcact gacaatgaga tccaagcacc
    17581 tatggataaa ggaacaaaaa tcggtaccgc aacgatcacc ttggcagaag ataaattagg
    17641 ttatcttgaa gacaacaacg cgccatccac agaaatcatc acggataaaa ccgtagaaaa
    17701 agcaaatatt ttcgttattg gctggcgacg cgtcgcagaa tttttcggta acttgtttta
    17761 attgaacaaa aagaaaaaag catctttcca tgtggaagat gtttttttgc tttttctagt
    17821 ttaaataaca gcttttttca tctattttcg ttatactgta taagaaaaag gagttgaatt
    17881 tatgaatcta accagagatc tgattttttt ggttctctat cttattgggg tattctccat
    17941 cgtttattgg cgaaaacaga aaaaggatcc ttccttgttc ttttacagca gtacatggac
    18001 gtttgcggta atcttgactt attttgtcgt gttggaaatt ttttattcga ccttttattt
    18061 tttgattccc ttagtgtttt tcagtatctt tgccttttcc tatttttcag aaaagcgaag
    18121 gctgttgaat ggtcttttat tcaatatttt cctcattagt tttggtatct acttgtttgt
    18181 cttgctatac gaaacacaaa atatttttct tggcggactg attgccttga tcacgatccc
    18241 gttactgctt gtctttctat tcggcattta tggattgatc gtttttcttt tttggaacgg
    18301 tgtcacagtt ttacgtagag agtcgcattc attagctaat cttttaacgt tgatactagc
    18361 catttttctt acattgtttc ttgtttttga ctttttcttg ttaaaatatt taccacaatg
    18421 gataaatgct ttgtttttct gcatcccatt gatcttgatc tatctattta tcgtattcta
    18481 caactttttg actgtctcat tcttgtatca gttcaaccgc cctcgataca accaagactt
    18541 catcgttgtg ttaggcgccg ggttgatcaa tggagaaaca gtttctcctc ttcttgccaa
    18601 gcgaatcaat aaggcgattg ctttttattg ggcacaaagc cgtgcgacat taaatccgcc
    18661 tatcctatta atgtcgggtg gacagggagc agacgaaaaa gtaccagaag caatggcgat
    18721 gaaacaatat gccatggaac aaggaattcc tgaaagagat attcttgtcg aaaccaattc
    18781 gacaaccact ttggagaata tgcgctattc aaaggaaatc atggatcagc aaatgaaagg
    18841 tccctatcgc gcgatcttca gttccaacaa ttaccacatt ttcagagctg gtctctatgc
    18901 acgacaagcg aaactgaagg caaatggcat tggggcaaaa acagctttct attacttgcc
    18961 taacgcgttt ttaagagaat acatcgccat cttgatgatg aacaaaaaaa gacatatgat
    19021 catttgcgga ttgcttttcg caggttccgc attcttatca ctgatcactt tacttttcta
    19081 aaaaaacaag gtgtgacaaa acatatgtca caccttgttt ttttctttca ttctcttaac
    19141 tattggcaaa aagagtatcc cttttctgac cgctacttgc tgagcagaat aaataccgct
    19201 gttgcctgaa cacataaagc tgatcaagca gatcataaag aagtatactg ccgcttgtga
    19261 accgaacaat tcgatcccca tcacaaagca ggcaatcggt gtatttgtcg caccagaaaa
    19321 tactccgata aagcccaatc ctgcaagaaa aggaacagaa acatggagca aaggcgctag
    19381 tgctgcacca agcgttgcac caatttcaaa caatggtgtc acttctcccc cttgatatcc
    19441 cgcgcctaaa gacaaaactg tgaagaataa cttcccaata aaatcataga catgtgcctg
    19501 tccattaaat gattcctgca gtaatggaag actcaatccg agataacgtt gtgtttgcaa
    19561 aatcaatgct gcggctacaa ctacagcccc acctatcgta tttcgccaaa caggatgagg
    19621 aatccattgt gcatatcgcg cttttaaaaa gacgatcgaa cggctgaata cccaaccaat
    19681 caaaccaaaa gcaatcccag caacaaaaac tttgaagaac agcaacatac tccattcagg
    19741 aatcacaccc atttggtaat gtagatgaga aacaccatag ctttctgtca taaaatttgc
    19801 aaacagtgca gcaaagaagc tagggaagat cgcttctgtt cggactttcc cgattgccaa
    19861 cacttctaat ccaaataatg ttccagctaa aggtgtgcca aatacagaac taaaacccgc
    19921 actgattcca ctgataatca agatttcccg ttctgattta tccaatcgga acaaacgtgc
    19981 tgtattatct gctaacgctc cccccatttg gactgctgtt ccttcacggc caacagatcc
    20041 tccaaataaa tgggtagtta tggttccaaa cagtgtaagt ggaataagtc gcaacgggat
    20101 tttttcttct ccaccatttc cttgatcgat cactaagttg tttccacgac tagaaagacc
    20161 gccgtgatac gcatagagat aagcaaaaag cgcaccacta actggtagta gataaaggag
    20221 ccaaggatgt gatagtcgga tatctgtcac aactgtcagg ctatttaaga aaaaagcaga
    20281 taagctcccc atgaatatac cgagaatcgc tgtgatcacc agccacttca gcatgtacac
    20341 taaaatgcgt actggactcg gtaattgttt ttccataaaa aattcctcct caataaaaaa
    20401 aggctctata atttatg
//
LOCUS       DAYYGO010000050        20346 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000050 DAYYGO010000000
VERSION     DAYYGO010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.051"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(9..314)
                     /locus_tag="ACHA5T_001477"
     CDS             complement(9..314)
                     /locus_tag="ACHA5T_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFM8887056.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(339..770)
                     /gene="ruvX"
                     /locus_tag="ACHA5T_001478"
     CDS             complement(339..770)
                     /gene="ruvX"
                     /locus_tag="ACHA5T_001478"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFM8887057.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(767..1033)
                     /locus_tag="ACHA5T_001479"
     CDS             complement(767..1033)
                     /locus_tag="ACHA5T_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFM8887058.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPSYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(1288..2013)
                     /locus_tag="ACHA5T_001480"
     CDS             complement(1288..2013)
                     /locus_tag="ACHA5T_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFM8887059.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(2374..2444)
                     /locus_tag="ACHA5T_001481"
     tRNA            complement(2374..2444)
                     /locus_tag="ACHA5T_001481"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2410..2412),aa:Gly,seq:ccc)
     gene            2561..3313
                     /locus_tag="ACHA5T_001482"
     CDS             2561..3313
                     /locus_tag="ACHA5T_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFM8887060.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNNLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            3329..3808
                     /locus_tag="ACHA5T_001483"
     CDS             3329..3808
                     /locus_tag="ACHA5T_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFM8887061.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(4059..5639)
                     /locus_tag="ACHA5T_001484"
     CDS             complement(4059..5639)
                     /locus_tag="ACHA5T_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFM8887062.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(5632..6375)
                     /locus_tag="ACHA5T_001485"
     CDS             complement(5632..6375)
                     /locus_tag="ACHA5T_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8887063.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(6551..7327)
                     /locus_tag="ACHA5T_001486"
     CDS             complement(6551..7327)
                     /locus_tag="ACHA5T_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM8887064.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(7575..8411)
                     /locus_tag="ACHA5T_001487"
     CDS             complement(7575..8411)
                     /locus_tag="ACHA5T_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFM8887065.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(8422..9351)
                     /locus_tag="ACHA5T_001488"
     CDS             complement(8422..9351)
                     /locus_tag="ACHA5T_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFM8887066.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            9543..9896
                     /locus_tag="ACHA5T_001489"
     CDS             9543..9896
                     /locus_tag="ACHA5T_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFM8887067.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(9970..10626)
                     /locus_tag="ACHA5T_001490"
     CDS             complement(9970..10626)
                     /locus_tag="ACHA5T_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFM8887068.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            10852..12786
                     /locus_tag="ACHA5T_001491"
     CDS             10852..12786
                     /locus_tag="ACHA5T_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8887069.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(12872..14095)
                     /locus_tag="ACHA5T_001492"
     CDS             complement(12872..14095)
                     /locus_tag="ACHA5T_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8887070.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(14089..15960)
                     /locus_tag="ACHA5T_001493"
     CDS             complement(14089..15960)
                     /locus_tag="ACHA5T_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFM8887071.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            complement(15981..17099)
                     /locus_tag="ACHA5T_001494"
     CDS             complement(15981..17099)
                     /locus_tag="ACHA5T_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8887072.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(17182..18015)
                     /locus_tag="ACHA5T_001495"
     CDS             complement(17182..18015)
                     /locus_tag="ACHA5T_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8887073.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(18008..18925)
                     /locus_tag="ACHA5T_001496"
     CDS             complement(18008..18925)
                     /locus_tag="ACHA5T_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8887074.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(19152..20276)
                     /locus_tag="ACHA5T_001497"
     CDS             complement(19152..20276)
                     /locus_tag="ACHA5T_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8887075.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT         6280 a         4045 c         3387 g         6634 t
ORIGIN      
        1 tttatagtct attcttcttc ttccgccatg aatgtattga atacttcttc aatcatgtcc
       61 cattctgcgt ctgtttcgat ttgttctaac tcgccttctg ttccatcttc gttttcgatg
      121 tatgcataag cttgtaattc tacatcttca tcttctgaaa caccagcagg atataaaaga
      181 acatagttac gtccaaattc ttcttgtccg tcaactgtta gtaggatttc atataatgtt
      241 tcatttcctt ggtcatccac caatgtgatg tgttcgtgtc cttcatggtc gtgggaatgg
      301 ttatgttctg tcataagatt acctcttttc ttttattatt aaaataattg cttacctgag
      361 ccgtctaaat aattttgtaa gatcatcaca gctgctaatt tatcgatgac tttttttcgt
      421 ttggctcttg aagtatttgc ctgctctact agcatacgtt ctgcctgaac agtggttaat
      481 cgttcatctt gataatcaac tggcaaggag aatagttctt tgatcttttc tccgtatgac
      541 atggaagctt ctgcccttgg tccgatggaa ttgttcatat ttttaggtaa tccaactaca
      601 aaccggtcta cttcatattg tgctactaat tccttcaacc gatcaaagcc aaactcttct
      661 tctgcttcat tgatccgaat gatctctact ccttgagctg tccagccaaa ggggtcactg
      721 atagcaacac ctactgtctt agaaccgaca tccaagccca ttactctcat aaattcactc
      781 catgatttga gaggtagtcc ttgacaatta cttccatgat ctcatcgcgt tcgtgacggc
      841 gaatcaaatt tcgagcatca cgataacgag gaatgtagga cgggtctcca gaaagcaagt
      901 agccgacaat ctgattgatt ggattgtagc ctttttcttc caaagcccga taaacaatcg
      961 ctaatgtttc gctcacttct tttttgcgac tatcgtcaaa accaaaacgt acggtttcat
     1021 ctgtaaaacc catgattcta cacctctttc ccgcaaggta attccagctt accttctaca
     1081 accttatttt actcgaagaa agggaaaact acaaacaaat attccaaact tccgaataaa
     1141 ttaagaaaca ctatttgtat gtttttttcc ttttttctgt agctttatca aaagtactct
     1201 tttcataaag tatacgctat atttctttga atgtatgttt ttacacaatt ttttcataat
     1261 attcataaaa aattcgaata gctgctacta attttttgtt tttgttgctt gtcctgtctc
     1321 ataattgatc gtgtagccat cttgaacatt gtagataaag acattgaatt caattttttt
     1381 gtcttcaatg ctttctgctt ccaattgcac tcccctggct accagttcat tcccctcaaa
     1441 atgaggagtg acacggtagc gtacatgatg atttgtttct ttgatatatg ccgcaatatc
     1501 attttcgtga gcaagcatgt atggagcatt caacgaacgt gtccctgtca tcaagttttt
     1561 aatgttgtta ttttgaccag tcagctggaa accaattaaa tggctgcgat tatataacca
     1621 ttgcccgtca cttaatttct tttgtttcca acctgtcggg tcaatataca aaggctctct
     1681 cttttcagta gggaacaaat cttttccaag catagcattg gcttgtccta ctctgttcaa
     1741 tttatcaata tttgaatagc tttcccatgt tcctttggaa aggctaagat cctcttttgt
     1801 aaattccggc tgattcccat tgatttctat ttcttgatgt tttccatcat attctggcac
     1861 atccgctact tgagaagctt gtggtgtgga atcaaaaata gttgtaatct gctgaaagat
     1921 atctgaatct ttttgtgtgg tggaaacacc gaatgcccct gcaatcacag cagcgacgat
     1981 ccccacgatc gccttggttg tttttttgct catttcttta ctcctttact aacagctccc
     2041 taaatcttaa catatgttcc cctataaata aatactagaa agaaatagaa acagcaaaaa
     2101 ataaaacctg ttttaagatg gcttaaaaat acaaaaaaga gacggattcc tcgtctctaa
     2161 tcagtcacca ctctatctgt aaggtattta ttattattca aaaattttct tcatactcaa
     2221 gacaatacac tgatttcaca accgcttggt aaacggtgcg caaaagcttg catctgcggt
     2281 aataagtcca tccaagttaa aaacataaga agttgttttt gcttggctgt tcttattact
     2341 tgttgcagag cagctttttc acaacctatt ttaagcgggt gacgggaatc gaacccgcga
     2401 catcagcttg ggaagctggg attttaccac taaactacac ccgcaataca aaaacagtat
     2461 acgcctcttt tattccttcc tcaagttttt ttattcttta cgcttctggt atgttttgta
     2521 gtaaaataaa actaatcaat caattattgg agaagatgct atgacgggaa aagaattaga
     2581 tctgttgctg gacacttgtt tactcgctgg gaaaatcatg atggaaagca atgcggagat
     2641 gtatcgggta gaagatacga tgagtcgaat tgctttagct tctggaaatt tccgtttggt
     2701 cagctatgtc actcaaaccg gtctctttat tggtcttgat cgtacctcga ccatccgaat
     2761 ggaacaaatt accaaccgtt cgattaattt agaaaaagta gtaaaagtaa ataatctttc
     2821 acgtaaatat gttgcttcag agctaacatt agaagaactt tactgcgaac tgcaagcaat
     2881 cgaccgagac cgttctttct ttcctgtctg gttgcaaatc gttagcgcgg cagtgatcag
     2941 tggaacaatt atggtgttat ttggcggaaa gatgaccgat ttacctgtga ctttagtcat
     3001 tggaggattg ggatacgcag cttatctata tagtttgaaa ttttttagaa tcaaattttt
     3061 atcggaattt ttatcttctt tattgattgg catagccgct atctacagcg tacgttttgg
     3121 cttaggaagc aatcaggatc taatcattat cggatgtgtg atgcctttag ttcctggagt
     3181 tcaaattacg aacgctatcc gtgatttgct agctggacat tatgtctcag gagtttctcg
     3241 cggaacagaa gcgatgatga ctgctacgat gatcggtttt gcgattgctt ttattttcca
     3301 gcttttttat taggaaagga acacaagtat ggggacgata tttattcagt tttcttttag
     3361 ttttttagcg actgcggcat ttgccgttat tacaaatgta cctaggcgtt cattgatttg
     3421 ttgtggtctt tctggtgctt ttggctggat gatctactgg atctgtgttc aaataggcgg
     3481 atctgctgct tttggttcat tgcttggttc tttaggagta gcattcatca gcgatctttt
     3541 ctctaaacgt ttgaaaatgc ccgtaacgat tttcaatatt cccgggatgg ttcctctagt
     3601 ccctggtggt ttagcttatc aagcggttcg aaatctcgtt actgaatcct atcgagaagc
     3661 ggctcattat gcggtccaag caattatgat tgctggagca atcgctttag gtcttgtatt
     3721 atcagaagtc tttaaccata acatccgtaa tttcaaagta aagcgtgacg caatcatgat
     3781 gaaacgaaaa aaacggcttt ttaaataaac gaagtgtaac aaagaaaagg ttgtgaaaga
     3841 gacgttcagc cttgagcgat gacaaaatat ctcacaaaca ttgcgacaag taaccgcagg
     3901 agcaataaga agtaattatg agtattaaaa aaagttttgt aaaaatagct tctcacattt
     3961 ttacataaaa ttgacttaat actgagaaac tgacttttga acaccctcta tctgtaaaaa
     4021 aggggctgcg acatgatttt tgtcacggcc cctcttcttt attcaaattt tttccaaaac
     4081 gttcgttgat aataaacgat ccatagagcg gaaagtatta gaactaaacc gatcacagga
     4141 atattcacta accaaaatgg ataaaacaaa gccaacattc cgtataagac gataagaata
     4201 atactgatga tcaatgaacc catcatagtt gcgactaaac ccaaacttcc tgaacctctg
     4261 ttaaataatt ggttaatatt ggtccagttc agcagcagca accgataatc tctagcgaaa
     4321 taacgtagtg acaataagta gcagccgaat aaagaaccaa atagcaagct agggataaaa
     4381 aacaaaggca gctgaaaaga cagtccgctt aataatgcga tcccaccagt taaaaaactc
     4441 tgcaatatcc atccgaatct gaacttttct tttaaatatt ggttcataga gattggcaaa
     4501 gaacggataa acagaaaatt ttcttgatcc aaagaaataa gattgctgat aaaagaagtc
     4561 tgattaactg ttaagaaagc caatgcgatc cctaccagga aaacaacacc gattaactta
     4621 ttatctaaat cagttaattt tatttcgcct cctagggcaa aagcaagaat aaaaatgatt
     4681 ggagttaaca aggaagaaga aagaacttgc ataatcaaat taggttcttt tactaattga
     4741 gcattgtaac taaataaaag ctgatgcaca ttttggttct ttttatgggt tctttttatt
     4801 ttgaactcac tgtttgaatc agtcagttgc tctgtaagct ttggtactat cagctgtttt
     4861 acagcaagga aaaatcccag agccgtgata gctagtattc cccaacccaa taaaccttgt
     4921 tgagaaagtg gctgactggc agcatcatag aatggcaata atatcgaaat cggatgccga
     4981 tctgttaact cattttcaat agtccccgtt tgatgattca tccacatgat tccagcaaat
     5041 gcgatcagca tcgagctgcc aagcaagata ctagaaactg tttttctatg ttttttaaac
     5101 actgctgttc gagccaaacc aaaaacaatc aaactagcca gacaaaaaag aataatcaaa
     5161 aatagagaaa acaaaaggat tgagataatg atagtaagca aaatccctag atcagctcgc
     5221 catccggtta aaataaacat gactagaagc ggaaaaacaa aaggaatgat ggtcaaagca
     5281 actaccaaaa tctttgctga aaaaacctgt tcttgtccaa aaggcagagg caaaaatact
     5341 tgcaggtcat ttccttcaaa aaagatatta taaatgactg atatactttg agttaaacct
     5401 aacataccaa aaagtgccac gtaataagta aagaaacctg gcaatttaac aaagttcata
     5461 gcaaacatag caaatccgta aatcagtaaa aaaatcagcc ctgaaaaaag attttgagac
     5521 aaaagataga tagttaatcg ctttcccttt tttcctttct ctcttgcttt attcgtgagc
     5581 tgtggattaa catataaaag attaactcga attaattcta ataattgtcg cttatacata
     5641 cggtccatct cctgtcgctt gccttccagc cattttcaaa taaatctttt ctagcgtttc
     5701 atcaggtgag ccattcaata attcactaac tgccccctga taaatcaatt cacctttctt
     5761 caaaatagcc agtttatcgc ataactgttg tgcggtatcc aatacatgtg tagaaaaaat
     5821 gactgttttt ccttttgcgg catgagcctt catcatttct ttcaaatcaa atgctgcttg
     5881 aggatcaagt ccttgcaaag gttcatccaa tacccaaata tctggatctg gcagcaacgt
     5941 gccgatcaaa atcgtttttt gacgcattcc atgtgaaaaa cttgcgattg tttcattttg
     6001 atgactatac atatcaaaaa gcgctgtcaa ttctgctaaa cgcttttctt ttttttgtgt
     6061 gtctaattca taggcagctg aaatcaagtc ccaatattct ccagccgtca attgtaaaaa
     6121 aatatctggg gaatcaggca cataaccgat tttttgtttg atactcaatc gattttccgt
     6181 caatttcatt ccatctacta aaatcgttcc cgatgatggt tcaataatac taactaaact
     6241 tttaatcgta gttgattttc cggcaccatt atgtcctaaa aatccaaaaa tttccccttg
     6301 tcttatcgag agagacaagt cttttaatgc ttgttttttc ccgtaacttt tacttacatt
     6361 gatgaattca atcatttctg tactcctttt gtttgttatt actttttatt gtacccttcg
     6421 ttattggtgg agtaaatgtc aaaaagagtg cataacaaaa aaggatagaa acgactaggc
     6481 tgctacttgg gttgttctct tctacaggaa aacactcttc taacaggtaa gatatgagtc
     6541 tttgttttct ttaaaatgat tcattttcta tcttttcttg cataaaattc tttagtttat
     6601 caatacttct ctttcccatc tctagttgtt ctccatttgc aaatgttact atttcgttta
     6661 ctctgctaat agatgtaatg ttttcagtat taatgatata agcttttaaa tctttacata
     6721 aatactcata ttcgagtgat tttttatatt ttttaattgt gccgtttacg agaatctctt
     6781 catctttcaa tactaggcct attttatttt tataacctgg catgctcatt agataaagga
     6841 tttcatcgta atggatatag tgatcgctat taccataacg gacagttgtc cacctttttt
     6901 tcgtagcatg ttgaacagga tagttttctt tttctttcaa taagtaactt tggatgcttt
     6961 ttttattttt ttcataagtg aattctttta tcaaataacc actaggcgta atctttcgat
     7021 taataacagt tattgcttta ttcggatgag acgttagata aacaatttta cacttttcat
     7081 tcttttctct aagcatttcg cctagttcga taccgctata ataggcgttc aaatcgatgt
     7141 ctaggaaaaa aatatctgag gaatagattt cattattttt aatgaattca acaaattgta
     7201 taacgttcaa tccctgaacg atctcaaatt tccaaggact taataaaatt ctggattctt
     7261 ccaaaatttc tactagcatt ttttcaaaaa ccaaactatc atccaaaata aatatacgcg
     7321 ttaccataaa aagtacctcc actcgatatt ttactatcaa aatcatttca tacaactttt
     7381 caacatacat gttaggaata aattgtaatt ctcttcacaa tcttttccaa cacctttgaa
     7441 accgatatca aatactttca ggatgctgct tctaaaataa atatcaaaca aatgaataca
     7501 tcatgcaaaa aaagccagac aaccgacgtc cttgcgtgtc agctgtctga gctatgttta
     7561 tttattttgt tcttttaaaa agaacgatac acatactgat ttttcggagc agagactttc
     7621 tcagcgtttg taacaactac agatgggatt tcacgcttga atggttgata atagtctgat
     7681 tggatggttc cttctatttt ataccattca tcatttttaa ccgttactcc ttccggcata
     7741 tgcgtcaaca aaccaaagac tcctgaatct gccacacaat ggataatgcc gaatcggaac
     7801 aagaagatat ctgtattgct atcgttcggt gtttggtaaa caaatccttc atagctgatt
     7861 tttttgccaa tgaattcgct agggtaatta taaattagct ccataacttc taaatagttt
     7921 tcatctgtga tcttgatagt ttgcttatcc ccatactttt ctttcaactc attcatttgg
     7981 tctaagtagt ctgatttgtt gaaataaata ctggtatccg gctttaagta ttgtgtctgc
     8041 atctccggat cccctactga ttctttactt actgggaaat taaatccttt ggcttctacg
     8101 atcgtagtgt cgagactaac tgttggaaac aacgtcccca cgattactgg caaagctagc
     8161 agtacataag cgattccacg ttgataaggt tttgacaacc catggtcatg atcgtgctcg
     8221 tgtgcctttt cttctgcttt tccgtttttg acccacaaat acagctgaac taaagccaaa
     8281 agagcagata aaatcattga aagaatagct aagtagcgat aatggacatt gatatattga
     8341 tcgagtttgc cagaaacttg caagtacatc atcaattcga aatacccaga taaaatcaaa
     8401 aaccgcagca tattttccct cctaaataaa taatgtgaat ccaataacgc taagggcaat
     8461 cagtccgatc agcgataaaa taaagcgtcc tgtaaaatat cgtttcatca tcagtagatt
     8521 tttgatatcg accattgggc caaagaccaa aaaggcagct acaggtgctg tgccgaacag
     8581 acttaacaaa gaagatccta taaacgcatc tgcttctgag caaagagaca tcgtaaaggc
     8641 caataacagc atgatgataa tggctaaaat ctttgttgag ccgagcgtta gcataattct
     8701 agttggtaaa taagtctgca tcgcagctgc gattaatccg ccaatgatca aataacgacc
     8761 tgtatcaaaa aattcgtcga ttgaatgtgt caacacatgc ccagtttgat ggatcagctg
     8821 attttttggt tcattcttct cactttgttc ttcttcatgg acatgatgat ggtgattttc
     8881 tgctacttta gacgtgattt tttctttcaa tacagcgtct ttttgaaaat aggcaagcca
     8941 gacaccaatc acaagggcaa caaaaaagct gcctatcacc cgccagcttg ccatctgcca
     9001 agaattgcca aatgcgataa aagtggaaaa aatcacgatt ggattgatga tcggagcagt
     9061 cagcataaaa gcaaacgctg tgtgaactgg tacgcctttt ttaacaaatt gatgaacgat
     9121 cggtacgatg ccgcattcac atgaaggaaa gaaaaatcct aagatcgaac cagtaaaaat
     9181 agagaagata cgattttcag gaagccatct cttcacacgt tctggggtca aaaatacttg
     9241 taaggcgcct gagataagac agcctaataa tacgaaaggt aatgcttcaa taacgatcga
     9301 taagaaaatc gtactcatct gcaaaaccga atttggaata aaagaaaaca tgagtttcct
     9361 cctttttatt tctagttcat tcgctttgca aatatacgct tttattcaaa aaaaacaagt
     9421 tgctctgtaa atttttacac aactcttatt ttttaccaaa gcacttcaat tgactttttc
     9481 tgttattccc attaaattaa ggtctcatac atatcacttc tatcataaaa gggagaataa
     9541 aaatgaaaca aaccactaac ttgactgaag ttcaagacat actgcaacag tcatccgttg
     9601 ccgtcattta tctttcgatg cctaattgca gtgtttgtca cgctgttcgt ccacgactag
     9661 aggaacttct cacacattat gatattccgg cacttcattt ggatgcattt gaaacaccag
     9721 aagtagctag taaatttgaa gtgctgactg cgccagttgt cctgattttt catcaaggaa
     9781 aagaagtctt tcggcaagca cggttcatcg actttaaaaa aatccgttat ctactagatg
     9841 aactgacagc agaagatgat tccctttctt atgaagaaat cttccgaaca aaataatcgt
     9901 tgtccaaaca gaaaaaagcg attgaacaag tgaaagtttt gccttcattt gttcaatcgc
     9961 tttttatttt tattctgctg tattttcgga atgcgcattt gcgtcagaat gcaagaaata
    10021 gatcaaggtt tgcaattcat tcgtcaaatc aacattttgg atcaatacat ctgacggagc
    10081 attcaatcga gcagcggtaa agttcaagat tcctttgatt cctgcttccg ttaactgatt
    10141 gactacttct tgtgctcttt cagcgggtat cgttaaaatt gctacttcga tttgttggat
    10201 cttgatttgt tctgacatat cttccattgg ataaacgggg ataccgtcta cgatgcgccc
    10261 aacgatttcc tcattcacat caaaagcaca actgatacgt atgctattac tttgatggaa
    10321 tttgtatttt agtaaagcac ttcccaagtt ccctacaccg atcagcgcaa catttgtcaa
    10381 ttgatcgtcg tttaatgtct ttgcaaaaaa gtgcataaga tcttctacat cataaccata
    10441 cccacgtttt cctaattcgc caaaataaga aaaatctcga cggatcgttg cactatctac
    10501 ttgaacggct tcacttaatt cagttgaaga cacttttttc tttccagcat tatttaaaat
    10561 ccttaaatag cgataataca aaggcagtct tttagctgtc gcctttggaa taatatgatc
    10621 tttcacgaaa tgacctccac gtattgtcta attacatctt tgatttctcc atcatagcag
    10681 tatttatagg aaaaaatcaa ttccattgct cagatattgt gaaattttat tctttattca
    10741 tttatttatc atttctgcaa atctcgcagt ataatggaat ttatggtaga ctactaattg
    10801 aaaaatagtt ttttatttga ggagaattca caaagtagaa aagaggattc catgatatta
    10861 ttacaagcca atcagattgc tcgccatttt ggtgcagaag tcttatttga aaacatacat
    10921 ttagaaatca gtacaggcgc tcgtgtcgct ttagttggca gaaatggcgc tggaaaatcg
    10981 actttattaa agatcattgc tggaattgaa gcacctgatg aaggaaagat cgctaaaaat
    11041 aaaacagcga ctctcggata cctcgctcag gacacaggac ttgattccaa tgaaacagtt
    11101 tggaacgaga tgctgaaagc ttttgaacaa gttagacaaa tggaagagcg gatgcgcgag
    11161 ctagagattt tcattagcga aggtacgcca gacacacctg cttatagttc ttatttaaaa
    11221 gaatacgacc ggctgcaaca cgatttttca gatcgaaacg gctatggtta tgaaaatgaa
    11281 attcgttccg tattgcatgg gtttaaattc gattcttcat tttatgatca gaaaatcgat
    11341 actttatcag gcggacagcg cacacgttta gctttagctc gcatgctttt gcaacaccct
    11401 gacattttga ttctagatga accaacgaac catttagata tcgagacact ttcctggctt
    11461 gagaattatc tgcaaagcta tactggtgca ttattgattg tttcccatga ccgttatttt
    11521 ttagataaag tagtcaatga agtctatgaa ttaagcagaa gaaaaatgcg ccattacaaa
    11581 ggtaactatt cgaaatatct agatttgaaa gcagaacagt tagcaagtga atggaaggct
    11641 tatgaaaagc agcaaacaga aatccataaa ttagaagact tcgttgctcg gaaccttgtt
    11701 cgcgcctcta ccacaaaacg agcgcaaagc cgcagaaaga cattagaaaa aatggaccgg
    11761 ctggatcgtc ctcaagggga tgaaaaacaa gcaaactttc tattcagtat agaaaagcct
    11821 tctggaaatg tagtcttgca aatcgaagat gccgctatcg gatatgataa tgtcacctta
    11881 tctgaaccaa tcaatctaga tatccgtcgt caagaagcga ttgctctagt aggtccgaat
    11941 ggtatcggga aatcgacgtt gctgaaatca atcattcacc agatcccttt tatccgtgga
    12001 aaagaaacgc tagggacaaa tgtcaccatc ggctattacg atcaaggtca agctgatctg
    12061 catacaaaca agacgatcct agcagaattg tgggatgagc atcctaccac tccagaaaaa
    12121 gagatccgaa gtgctttagg tgctttctta ttttctggcg aagatgtaga aaaaccaatc
    12181 cctctgctaa gtggcggtga aaaagcacgg gttgctttag ccaagttagc gatgaacaaa
    12241 gaaaattttt tgattttgga tgaaccaaca aaccatttag atattgacaa caaagaagta
    12301 ttagaaaatg cattgatcga ttatgacggc acactgcttt ttgtttccca tgaccgatat
    12361 ttcatcaatc ggatcgcgac aaaagtcgtc gaactctctg aaaatggcag caaagtctat
    12421 ctaggagact atgattatta tttagagaaa aagcaggaag aagaagaaat cgccgaattg
    12481 ttggcacaag atgagccaga atctgctcct gctcccacga acaaatctga ttattaccaa
    12541 agcaaagaac agcaaaagct tatccgtagc cttcaacgaa aaattactca ggttgaagaa
    12601 gaaatggcgc ggatcgatga atggatcgat cagttggaac aagaaatggt cgcgccagaa
    12661 aacctggaag atcacgtaaa actcaatgag ctgaaccaag atttagaagc tgctagacaa
    12721 gatcaagaaa caaaattgac ggaatgggaa gaactaagct tggaattaga agaatatgaa
    12781 aattaacgaa aaaaccaaaa cagaaaccat tcatgctgtt ttggtttttc tttttttatt
    12841 ttatcaaaca aaaattccat atttaccttt ttcaccaata agaagaccaa ttactagtca
    12901 ttctcactaa agcttctaaa taatagtaat caccccaaat acaaggtgta tttaccccat
    12961 tatttttggg cttattataa acagactgtg ttaaaaagcc atcttcttct tccgttgtag
    13021 catatctttc tttcaattgt cgcataattt tccaaacagc agactggtat aagttctttt
    13081 ctgttttgtc taatagacct gattctatta attctaataa tccacaaacc atgatcacca
    13141 tactagatgt atctttttct tctctgctag aatcatcaaa gatcaaatcc caataacaaa
    13201 tggtatcttc tggcaaccga ttcataaaat aattggctaa tattttgctt aaatcaaaat
    13261 atttttgttc tttcaaataa cgataactta atgcaaaacc gtaaatcccc catgcttggc
    13321 ctctagccca acaggaatta tcactataac cttgatgcgt acttccatat cttggttttc
    13381 catctttgac atccatataa aatgtatgat aagttgaacc atcttctctt actaaataat
    13441 ctgctgcatt ttgaatatga gcctgagcag cttttttata tttttgattt ccagtttctt
    13501 cactcgccca atacaataat ggaagattca tgcaacaatc catgatcatt ctaccttgtt
    13561 gtgaaggatc actaggatct ccccaagctt gtataactcc tgccttctcc caaaaacgct
    13621 gaagtaatgt ttctgcagca tcaattgaag cttccttcat ttttcgattt cccgttattt
    13681 tataatctgc tacggttgat aaagaataca aaaaaccaag atcgtgatga ttagtgataa
    13741 tttgacagtc aatcctgtgg ataaaatcta tatctgcttt ttcagctgtt tgttgaaata
    13801 aaggatcacc cgtcttctca aataacaacc aattaattcc tgtccaaaat ccttctgtcc
    13861 aaccaatatt gtctcttaat ggataacgtc cttttatagg acactcgctt gggaattgat
    13921 actgactgtt actgatacgt ttgattaacc tctccgtgtg tctgcgacaa cgctcgatct
    13981 cattattcca ttctttttcc attaattctt tataacattc atttatccat ttttctggat
    14041 gtattatctc ttcaaacatc actttatcaa gcatttcccc accagccttt agcataattt
    14101 tagtcttttt ttattgtttt tattttggat agttagcccg gttgtcccat caaaatcaat
    14161 tttgggacga ttgttccata cttctaaact ataatcccga tcttgatgag caaccactcc
    14221 tgttccgagc caatgggttc cttttccaaa tgtaccctct aatccaggaa taaaagtgtt
    14281 ttcccatgtc atcaagtttg tattgggcaa acccataata gctgtggatt ttcgtttaat
    14341 agtatcgtca cttaaacaga taatacctga aaagccagct tcactcaccc tatagttttc
    14401 cttgtcttct tctatgatta ttccttcttt ctcaggagaa cattcaatag caaaccctgt
    14461 ctcatataga acatattctg tatctaattc tactttgtgt acgcgaatct gccagccttc
    14521 aaaaggaata agccaagttc tgatttttgc ctgtaaagta ggttcccatg tagaggcaag
    14581 tacctctttc ttacatacaa tgtctcgtac attatttcga acgatgaact gtccataggt
    14641 tttgatgctc tcattcttaa aactggtcgt gatatatgtg tccgctcgct gaatccctag
    14701 cattgaatcc attgccattt ctgctttcaa ttgattcgct cgcggtatcg aaaaaccaaa
    14761 ttgactagaa taagcaaatt ttaaatattt ttcattggtc aatgtatgga aatttggccc
    14821 taattgtccc gcattcaaaa aaaatgtatg tccattatca tgttgtatga gcaaattggc
    14881 ttctgaaaga agtttagttc cttcacgaat aggcataggc atttctttag ccgtccaata
    14941 ctcatggtct tcaggtagcg ccaacaataa aaaaatctta ttcagccata atggagataa
    15001 cgtactatta tagggttcag tcaatgtcaa ttgagggtag gtataaccaa tattcaggat
    15061 acctttttca tcaaatattt ctttttgcat ccaccatctt atattccgat tgactaaccc
    15121 cttgatttca cctaatttaa aaggcaaaag atcggcccac aacatagctg accaaaagct
    15181 tgaaactgca aaacgataaa tcatgcttcg accaaaaggc acatttgcac catcttccgc
    15241 aaaaaaatga atatagtctc tagaaaaatc aatagctcgg ttcttatata tctggctaat
    15301 gggcttttct ggttctagta cgctataaat caaaccataa taatgaaatg caaaaggatt
    15361 ataataatct tggcgacctc ttgaactgtc tcgataccag ccattttgaa gataacaatt
    15421 ttctatatta tcaaatgctt ctttcagtgc tgattcttta actggataac caaatttttg
    15481 gataattgtt ccgacaatta tcttaaaaaa ttgccaatta ccatctgcac attcatattc
    15541 aaaaatttgc gagaaccaat gaataatctg gcttttttct cctgtatcta attttttcca
    15601 taataattgt tcatgatgat ataacgctaa taacattgcg ggcatttcta ccatacgctg
    15661 gtctctttca tgtatttctc cccaataatc gggatgggaa ggatctgttc cagcttttat
    15721 tcctgataga attaatttca accatcgttc atctaattgg tcaattgcgg gccccgctcc
    15781 ccataaaacc cgagagaatc cttccattcc tgcaatttta tttccatatc ctaccccttg
    15841 atgtatataa tcaatttgtg cgccacttgc tcgataaaac ggctggagtg gttgatttat
    15901 ctttttaaat aagtcaaagt aatcctgtct ggtccttaat agtggtactg gatgtttcat
    15961 tgtattttct cctcttttta ctaccaaata gctaattccg actgttctaa tcgaagtaag
    16021 ccttcaataa aaaaataatc agaatagatc agtggcacat tataagtcag ttctctatgg
    16081 tatgcttctg ctccgcctcc aatgatccca tcttcatcca tattccaatt tgcatagtgc
    16141 ttttctgtct tttggagtat attgatagca gcttcatgat acatttttga tcgtacagag
    16201 gaaacatatc ctgctaattc cagaagaccg catgcagcgc atactccagc actggtatca
    16261 atattcgaat cttttacagc aaaatcaatg agaggaatat aaccagtttt ttcgattttc
    16321 tgaataaaat ttactgctat cttttctgct atagctagat agtacggatt ttttgtgtaa
    16381 cgataactta acgcgaatcc atataaagcc catgcatttc ctctgctcca acaagaattc
    16441 tcattgtatc cttgcccgcc aagtaaccga atatatttcc ctgattctgc atcaaattct
    16501 gcgatatgcc cggttgatcc atctttcctc actagatact tagctgttgt atctgcatgc
    16561 tgccgagcaa ttttagagaa cctcggatca ccagtttccc tcgaagccca atacagtaat
    16621 ggtaaattca tcatactatc aataatcatc caaccaggtt tatggtcatt ccatgctact
    16681 aaatagttcc ctgaaatatt atagcgtcca gcaagtaccg tagctgcatg taaccctagt
    16741 tgtcgactat gtttgttttg agactgttga taatgtgcaa tagccgatgg taagaataaa
    16801 aaacctacat catgatgaac gtctgaaaaa tggtacaggg cagattctaa taattgttct
    16861 tgttgtattg ctgcctcttt gaacttttct cgttttgtct tgtgaaagag ctgccataaa
    16921 ataccactcc aaaaaccatt ggtccaccag tcgattcctc tgtcctttat tacgtctgta
    16981 tattttccgt tgtaagaaat atatggaatt ttattcccca ttctgtctaa ttccgcatct
    17041 atcttttcag aagctacatt caaaattttt cttgaccatt ccttcgtctg ttcattcatg
    17101 gatatcccgc cattctttta atttttatga atgtggagtt gctcctagaa cgaactagat
    17161 ggttaacatt cgttattttt tttacttatt tgcagctcca atgtcattct ccttaaattt
    17221 agactgcgcc cacacataaa tcaagaccat tgggatcact gaagttgcta aaattagcat
    17281 aattgcgcca taattaattg gctcacctgt actagaaacc gtcatatttt tgataattaa
    17341 gggaaccgta tatttatctt tatctgaaag catgactaat ggaaatagat attgattcca
    17401 acttcccata aagataagta aaccactagt aataatcgta ggtgataaaa tcggtacagc
    17461 gattttcgta aaaatcaccc actcattggc accatcgatt ctggcagcct ccatcagtgc
    17521 tgtaggaaat tgactgctat attgtcgcat cataaacact gaagttgtac tggcaatcga
    17581 agggatgatc acacctgcat aagtattcga taatcctaaa cgactcataa tgtaaaataa
    17641 aggaatcaat gtggtaaaaa aaggcaccat cctagatagc ataactgtat tgaacaacag
    17701 tttattcccc ataaaacgat actttgctaa tgcataaccc gccattgtag aactggctgt
    17761 agctaaaact gtactgatta cagcaataac aatcgaattc caaataatca agggataatt
    17821 atatctcgcc atgatggctt gccagttttt taatagcata tccccaaact tgaaattgat
    17881 tccgccactt aaaatttctt gattactttg cgttgaagct acaatcatat ataaatatgg
    17941 catcaaaaat attaaggcac caaacacaat aatgacgtac gtaaaaattc tgctttgtat
    18001 cttttgttta gtcattttta tcacctccga atttaaattg aatagccgat aatataacag
    18061 atacaaacac catcgtccag gcaatagcag aggcttttcc aaaatcaacc atttcaaaac
    18121 ttgttttata taaattgact cctacgacca aagctgaatt ttctggtcca ttggaagtcc
    18181 caaagaatat atttgggata ttgaaattac ccaagccatt gatagtggct aaaactgtgg
    18241 ccattaaaat gattggcttt agcatgggga tcgttaataa aaagaattgc cgaaatttcg
    18301 atgctccatc tatttcagca ctttcgtata tctctttaga aatcccttga agaccagcta
    18361 aaaataaaat agtgttatat cctacactaa gccaaatcat cgtgatgatc attgatattc
    18421 gtatccaaaa aggatttgtt aaccactgta caccgttaat tcctagcgca ttcaaaaatc
    18481 cattgaataa cccagtcgga ttgaaaaaag tcatgaatac ggaagctaca gccaccgttg
    18541 atgtaatggt tggaatataa tagacagttc taaaaaatcc ttttcctttt aactgtgctg
    18601 aattcaataa aacagcaaag taaatagcta atagcatcgt tattggaatt gatcctgcca
    18661 tgataatcaa aacattcgtg taagatttaa taaacaatgg atctcggaag atagctagat
    18721 aattgtcaaa tccaacaaat tcaaatgagt agccaatttt cataaaacta aaagcaaacg
    18781 agataatgat gggaatcaaa atcgtcacaa taaagaaaat aacaaaaggt gtgaccaatc
    18841 cataagcact ttttttatga gacgtatctg ttgctttttt cttgctgttc tttattgtct
    18901 tgcttggtaa cgaagattgc atcatcgtta agtcctccta atatttttta atcaccagtt
    18961 aattttacgc cgtacttatg agcaaaattt tctgcttctt ttagatatga attttcaaaa
    19021 tcacctgttt gccaatagtt atatgcagcg gtagtcaaat aagacgtagc atctgaataa
    19081 gtggctggta aaacagtatt tgatgtattt tcattgattt tattcaatag ttcatagtat
    19141 ttttgtcctc cttagtagtc aaactttttc tcagctgctt ctgtttgata ggccaatgag
    19201 ttagatgtag ccacgcccag ctccagtgcg ttggctaagc attcttcatc tgtcagcata
    19261 aatgtaagaa attgttgtgc cacttgtgga ttttttgcat ctttagggac ataaaaactt
    19321 gatcctcctg atactgcacc ttttcctgga ttttcttttg aaaaaggaat cagtggagcc
    19381 atgcgccatt tgtcagatgc atcaggaaaa ttcaaagaca tattcgtagc ataccagccg
    19441 ccagcaacaa acattgcaga ttcttgaaat gctttctcag catcttgtgc accgtagaaa
    19501 tgtacaatat ctgagtcgat catttctttg attaactttg cagcatgttt cgcttcttta
    19561 gtattcagac ggatatttcc ttcttcatca agcaaagatt gattctgctg ggctagcatg
    19621 ttgataaaca attcttggtc cccagattct ggcaaggcaa ttccaaaact accagttttt
    19681 tcttttaatg cttttgccac ctggatatat tcttcccaag aactgatttc ttcaaaatct
    19741 acacctattt cagcaaattt ttttggttga taataacaaa gtgctggggt aaaatcacta
    19801 gcccaaggaa taatcgtttc attactcgtt tgttgacgca aaatattcaa tttttgatca
    19861 taaaactgat cgccatattt gtcataaaat ccatattcat cagcactata aaaagcatct
    19921 tgaaattttt ccagcacacc tgctgctgaa gtgtctgcca tggagataat atccggcata
    19981 tcttcgtgtg ttaccaattt tggggtgatc atttgtactg ttgctgttcc cgaagctaca
    20041 ttcacaattt ttaagtcaat atttgctcca gttttgcggt tatagatttt tgcagcattt
    20101 tcaacatagt ttgtcagttt gccgtttgcg acatatactt ctaatgtagt ggcttttttt
    20161 atcattggat ctgcgatgtt tgctgttact tcttcgttag tttttccaca tgcacttaag
    20221 ctgaataata gggtaatgtt tagcaaagct atcaccaatt tgtatctttt tttcatgtct
    20281 atcccttctt tcttttcaag ttcttatata aaacgctttc acttatgtat attaacggaa
    20341 ttagta
//
LOCUS       DAYYGO010000051        20181 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000051 DAYYGO010000000
VERSION     DAYYGO010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20181
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.052"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            208..1014
                     /gene="proB"
                     /locus_tag="ACHA5T_001498"
     CDS             208..1014
                     /gene="proB"
                     /locus_tag="ACHA5T_001498"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFM8887076.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            1011..2258
                     /locus_tag="ACHA5T_001499"
     CDS             1011..2258
                     /locus_tag="ACHA5T_001499"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFM8887077.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            2334..2678
                     /locus_tag="ACHA5T_001500"
     CDS             2334..2678
                     /locus_tag="ACHA5T_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887078.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(2795..3121)
                     /locus_tag="ACHA5T_001501"
     CDS             complement(2795..3121)
                     /locus_tag="ACHA5T_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887079.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(3494..4696)
                     /locus_tag="ACHA5T_001502"
     CDS             complement(3494..4696)
                     /locus_tag="ACHA5T_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8887080.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            4813..5328
                     /locus_tag="ACHA5T_001503"
     CDS             4813..5328
                     /locus_tag="ACHA5T_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8887081.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(5527..5973)
                     /locus_tag="ACHA5T_001504"
     CDS             complement(5527..5973)
                     /locus_tag="ACHA5T_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFM8887082.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(6020..7498)
                     /gene="msr(C)"
                     /locus_tag="ACHA5T_001505"
     CDS             complement(6020..7498)
                     /gene="msr(C)"
                     /locus_tag="ACHA5T_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFM8887083.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(7852..8481)
                     /locus_tag="ACHA5T_001506"
     CDS             complement(7852..8481)
                     /locus_tag="ACHA5T_001506"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM8887084.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGVEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(8465..8929)
                     /locus_tag="ACHA5T_001507"
     CDS             complement(8465..8929)
                     /locus_tag="ACHA5T_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8887085.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(9416..10684)
                     /locus_tag="ACHA5T_001508"
     CDS             complement(9416..10684)
                     /locus_tag="ACHA5T_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFM8887086.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(10915..11652)
                     /locus_tag="ACHA5T_001509"
     CDS             complement(10915..11652)
                     /locus_tag="ACHA5T_001509"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8887087.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(11719..12288)
                     /locus_tag="ACHA5T_001510"
     CDS             complement(11719..12288)
                     /locus_tag="ACHA5T_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFM8887088.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLLVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12416..13288
                     /locus_tag="ACHA5T_001511"
     CDS             12416..13288
                     /locus_tag="ACHA5T_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8887089.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            13630..14502
                     /locus_tag="ACHA5T_001512"
     CDS             13630..14502
                     /locus_tag="ACHA5T_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8887090.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            14499..16457
                     /gene="nagE"
                     /locus_tag="ACHA5T_001513"
     CDS             14499..16457
                     /gene="nagE"
                     /locus_tag="ACHA5T_001513"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFM8887091.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(16708..17559)
                     /locus_tag="ACHA5T_001514"
     CDS             complement(16708..17559)
                     /locus_tag="ACHA5T_001514"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8887092.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            17682..18149
                     /locus_tag="ACHA5T_001515"
     CDS             17682..18149
                     /locus_tag="ACHA5T_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFM8887093.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(18412..18894)
                     /locus_tag="ACHA5T_001516"
     CDS             complement(18412..18894)
                     /locus_tag="ACHA5T_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM8887094.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(18994..19857)
                     /locus_tag="ACHA5T_001517"
     CDS             complement(18994..19857)
                     /locus_tag="ACHA5T_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM8887095.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         6067 a         4156 c         3662 g         6296 t
ORIGIN      
        1 tactcctatt ctaacatatc cgaggctgta aaagactttc tttacaaatt cttcaagaaa
       61 ttctgctatc ttaaagtaaa ccattaattc gttctttttc caaacgacta caagttgtac
      121 aaaaaaacta ttttttacat gactttactc gtcaaaacaa gtagaataga acgaaattaa
      181 tgagtggata tttgattgag gtgtcacatg cgtaaagaaa ttaaaaatgc aaaacgaatt
      241 attgtcaaag taggaacaag tacattgatt tatcccaatg gaaatatcaa tcttgcggca
      301 atcgatcagt tagcttttgt cctttctgat cttagaaatc ggggaaagga agtgatctta
      361 gtctcatcag gagctgtagg cgttggtatg cataaattac atttgtcaga acgtccgact
      421 ttgattcctg aacagcaagc tgcggcggct gttggtcaag ccgaactgat gaatatctat
      481 aatcaaagat tcagtgtata taatcaacag acggcacaga tcctcgtgac aagagacgtg
      541 gtagattatc ctgaaagtcg taaaaatgtg aggaatacat tggaacagct tttgattatg
      601 gacacgatcc ctatcgtgaa cgaaaacgat actgtagcag tggatgaatt agatcatctg
      661 acaaaatttg gcgataatga tcagctgtca gccatcatgg caaaacttgt cgcggcagac
      721 cttttgatcg tcctttctga tatcgacggg ttttattcag ataatccgac tacaaatccc
      781 gaggctcaga tgtatcgcta tatcgataaa atcgatgaaa agattctggc acaagcaggt
      841 ggtgctggta gcacatttgg aacaggcggt atgcagagta aattaaaagc cgcttctagg
      901 atatttgaag caaacagcgc aatgatttta gcaaatggaa aaaatccggc aattattttt
      961 gatattcttg caggaaaaga agtaggtact ttatttaagg aggacttgaa atgactgact
     1021 tgatgcaatt aggacaacag gcaaaagata gcgcgcaaca actagcactt atgcctacaa
     1081 accgcaaaaa tgagctactt gttcaaatgg cacaaaccat caaaaagaat caacaagcga
     1141 tcattgaagc aaatcataaa gacttagaaa aagcagtcga aaacaatatc agtgaaacga
     1201 tgcaagaccg cttgcagctg acacctgagc gaatcaatgc catggctaca gagatcgaaa
     1261 aaattgcttc gttagacgat cctattggaa aagtagatga gatgtggacg aacaccgacg
     1321 gattgagaat cggcaaaaag cgcgtgccac taggcgtgac cggcatcatt tacgaatctc
     1381 gtccaaatgt cacgacagat gcagctagtc tagcattcaa atcagggaat gccgttatcc
     1441 ttcgcggtgg caaagaagca ttcttctcga atcaattatt agtccaatta ttgcagcaag
     1501 tactggtcag tgctggcgaa tctccttatg ccatccagtt tgtcgatgat acttctcatg
     1561 aaactgcagg acagttgatg cgattgacag aatacttaga tgtgctgatt cctcgaggcg
     1621 gagcaaaatt aatccaacga gtcaaagaac aggcaacggt acctatcatt gaaacaggta
     1681 caggaaataa tcacgtatat atcgataaag aagcacagtt atctatggca gtgaacatca
     1741 tcgtcaatgc aaaagcttcc cgtccatctg tctgtaacgc ggcagaaaca ttgctgatcc
     1801 attctgagat cgcacctttt ttcttgccag ctattgaaaa agaactggta gaacacgggg
     1861 tctctttacg tgcggatgct cgtgcgttag aatatctaga gacggctgct ttagcagaag
     1921 acacagattg ggatactgaa tatctagatt atatccttgc tgtcaaagtg gtcgattctt
     1981 tggatgaagc aattgcacat atcaatcggc ataatactaa acattcagaa acgatcgtga
     2041 cagatagtta tgcggctagt caacgtttct taaatgaagt ggacgcggcg gctgtctatg
     2101 tcaatgcctc cactcgtttt acagacggat cggtatttgg ttttggtgca gagattggta
     2161 tctctactca aaagttgcat gcccgtggtc caatggggtt aaatgaatta acttccacaa
     2221 aatatatcat ttatggcgat ggacaaatcc gcgaataatg tttcgtgtga aacattattc
     2281 ccgccttttg ccttttttct gtaaaggttc tatccaacta gaaaggtgtc aatatgtttg
     2341 aaattttaat gatttttttt atttacctta tttctctgaa tatcgctgct tttctgggag
     2401 tcggtatttt atcccttttc tttcaattca aaaaacgaag catagggagt caacgggaaa
     2461 aatggtcgca gtattttgac aaaatcgggc aaaaagggct ggttacacgc ttgcacatta
     2521 gttacatggt tgcattatgc ttattagcga caatcaatta ctattctttt ttcgatcatt
     2581 caatcgctta tacgattacg ctactgattg ctgggatttt tcacttatct tataaatatc
     2641 agctgaataa aaaccacttg aatcggactt tccgctaaaa aatatccgta cgataagtag
     2701 agtgccttga agatcggcaa aaaactgccg gactttttag aggctgtgac aaagattttg
     2761 tcacagcctc tttttccata taaacggcgt tcgcctacca gctgcctggt aaatctgttt
     2821 cctcttcgtt aagaagcgaa aatccgtcaa atgtttcgcc atcataataa cgaagcagct
     2881 catccatgga caagttcatc gttttttctt tttcttcttc cgctgattca gccagtagct
     2941 gttcccgata cacctgatac ataccagcta ttttttgctg catcgctttt ctagtttcac
     3001 cttccgcatt gattattggc aattctttga tatagccacg ataaatcaac cgatcatctg
     3061 aaaattcaat gggtaattca acgagtgtca acccgacttc ctgaaaatat tcttccagca
     3121 tctattatcc ccccattctt tttcttaaat gaattttagc ttacctactg aaaattttcc
     3181 agtttcatta ttttgagaaa aaaagggaat gtgacaaaaa tcgtgtcaca tccccttttt
     3241 ccgaataaat ggtgttcttc tgccaactcc tcaatcataa gtcacaatat tcaacaaaca
     3301 tgagaaactg ttttttgaaa ttgttccttg tgattcagag ttaaacgaca gtctcacaac
     3361 ctcttggtaa acggcgttca aaagcttgca tctgcggtaa taagcccaac caaacgaaaa
     3421 atatgagaag ctatttttgt ttggctgttc ttatgacttg atgcaggaca gctttttcac
     3481 aacctctgat ttcttattct tcttcatttg tagcaaaata attacggtga taaatgatac
     3541 aacatacact gataagaaca atggcacctg caaaataaaa cgcatagctt tcaccaatat
     3601 gttcaccaat cacacctgca ataaatggtg ctaaagctcc accgaaaaag cggacaaaac
     3661 tatagctgga cgaagcaacg tttcgttgca gacctggtat ttccatagca atcgtcgtca
     3721 acagtgtatt gataattcct tggaaaaatc ctgctaacac aatgctgatc ggtacaagga
     3781 ctggcataga agttccagct cctaataaca gcaaacaaat aatgaataag acaaatgctg
     3841 ctaaaattgt ccgataagtc gtaaacttcc gctcaagagc cggtgcaacg aaaatcgaag
     3901 caatagccag aagcacaccc cagccaaaaa atacaaaacc gacttctaat tcagaatagc
     3961 caatcaataa aaatggcgca tacgcaagta aagtaaagaa tccgaaatta tataacaatg
     4021 cgattagtcc aactgaacga agtttatgat tgcttaacgc tttgacacct tcccaaaggc
     4081 ctacttttct ctttggtttc tcagtttcag gtaaaagtac cgctagggct ataaaaccgc
     4141 aaaacatcaa aaatgctacc ccaaaaaatg gataacgcca tgaaccactg cccaagaccc
     4201 caccgactag agggccaaca gccattccgc agccaagtgc tgcttcatac atcatgattg
     4261 ctttttctgt gttaccaact aaaatactga cgatcgttgc taaagcagta gagacaaata
     4321 aagcattccc gacaccccag ccggcacgta acccaatcag cattccgata ttcccagata
     4381 atccgccaag accagcgaat acaacgatga taaacaatcc tgtcatcaat gtttttttca
     4441 aaccgatccg actagataag aaacctgtaa atagcatcac cacaccagtg actaacatgt
     4501 aactagtaaa taacagggtc gtttcagctg gcgtagcgtt taactgtgca gcaatcgttt
     4561 ttaagattgg gtctactaga cccactccca taaaagcgat catacaggta gctgctacaa
     4621 ctagcgctga acttttcccc tttgtttctt catttgtttg ttcttgtata tctatttgat
     4681 tctccaatat ttccatccct ttctctctat tagcatcata catgatttat tttacatgta
     4741 aatatgcaca tgtaaaacgt ggaaacttta ctgtgatata ataaagaaaa taagacgaga
     4801 ggtaggattt ttatgaatac tttagcctat atattattat gcgtgctttt atccaaacca
     4861 cgttctgggt atgaattgaa gcagctgatc acaatttttt gggaagccca tcacagtcag
     4921 atctatacaa cgcttgccaa gttagaccag cagggttatg tgaccgtttt ggacaaagac
     4981 gagcactctc aaaaaaagat ctaccacttg actgatgaag gacgtgctct tgtagaggaa
     5041 tggatcaaag aggaaactcc cgctcctacg caaaaagatg aatttctagc aaaaatctat
     5101 gctttttcga cgctcgataa aacaacagct actggactgc tattcactag agaacaacag
     5161 ctgaacaaaa tcatgcaaaa aaatcaaaaa aaattgaatg acctctcgct agacaagaaa
     5221 caagaatttg gacgttatgt tgtgatccag cgcagaatta tgctttgtca gcaggaactc
     5281 caatggtgtc agcaagtaag agaacaaatg cagcagtttt ttgagtaagg gataaacaga
     5341 acctttctag aaatatctat tgaagagcaa attatcttca ataaaagagc aatacagttg
     5401 gaaaagaaaa agcgatccga acgacaggcc aaaacctatc gttcggatca ctatttttct
     5461 agaactgcat tcaagagact atgacaaaaa gcatgccaag gccttttttc aaataacagt
     5521 gttccatcat tcattacttt gaattgcttt tagcacactt cttgaaagcg ggccaacgat
     5581 gatcaactgc aacggtaaag cggcaatcac attcattttc caagcattca tataagcaga
     5641 gaataattga tcaggtattc cttgttccat gatcacaccg aataaagaca tacatgtcac
     5701 cattcctaaa accatcaagc atgaaatagt taagatcatt tggaccttgc tttctggatt
     5761 tactggtagt ttaaaggcaa tttttttcgc cagcacaccg atgatgaaga catccaaaat
     5821 gaaagccacc acgaatccag gcactagacc aatcaacaaa cttgataaat gaagatcttg
     5881 atgcagccat aaattatacg cgctcatccc caagaccatc aaaaaacaca tcattgttgt
     5941 aaaaattatt ccttcttttt tatttgtagg caatctgtac cactccttta aatttgtcac
     6001 gaaaatcatc gtagcacatt taaaaatttc tcgtaagtac tttttttatt ttctgccctt
     6061 ttatttcata aacttcatca gccgtacgct cgacaaagta agaatcatga gaagtaaaca
     6121 aaatcgttcc cggatatatt tgcattagct tctctaaagc ttcgatcgtt gccatatcaa
     6181 taaaattagt cggttcatct aacagcaaca cattacttgg cttagtaaaa agcaaagcaa
     6241 tcgtcagacg ggtcgcttct cccccactta atgtacataa cggtttcgtc aaagtttcct
     6301 gagcaaaccc taagtgattc agcaaactac ggacaagcga ttctgaaaac tctgtttgtc
     6361 tcatgacaaa acgaatgatg gattcttcag atgtcatttg ataacccttt tgctgatata
     6421 cttgaaagct tactttcgga gagagaagga ttccttctcc ttgtttgcgg atatgttcta
     6481 ataaggttgt cttacctgaa ccattttcac cgacaatcgc gattttttta cctaaagaaa
     6541 actgaaaatc accatttacc agtaatgttc tcccacttct ttccaattgg acattttgtc
     6601 ccatgatggg atagcggctg tggagttcaa gagattttgg tactggaaaa cggatttgtt
     6661 tcatctgctg cggcttggtt gtttctccta ttttttggag tcttctctct aatgttttcg
     6721 cattcttttg gatggctttt tgtacactgt ctttttgttt agaggaagac aaccgatccg
     6781 gtctgatttg ttgctttttc tttttactgg acactttttc caacttttcc gcttgttttt
     6841 tcttttcttg gatcgatttt tccaatcgtt ttttctcttt catgatcgac tctgcttctc
     6901 gtagctgagt ctttttctcc aattcctttt gagtgagata ggcactataa ttccccgaaa
     6961 actctcggat ttctccgtct tttacttccc agattttcga tgctagctca tctagaaaat
     7021 agcgatcatg actaacaaaa accagcgtac cataataata acgaagctct tcaatcagtc
     7081 gatcaatact ttttctatct agatgggttg ttggctcgtc tagaagcatc ccttgttcat
     7141 aagaagaaag aagacgtatc aactcgatct ttcgttcttc ccctccgctc catttccgct
     7201 ctgaagtttc ttccagtctt aactggaaat gactggctaa ttccaaattt aggtcctttg
     7261 cttcagcgag ataggttaat tgaggcaggt agttgaaggt gatctctgtt tggattttcc
     7321 catgatcggg aaaaagttca ccggctatta gtttgagcaa agtcgattta ccgacaccat
     7381 tttctcctat gatccctata cgatcttgct gatagacacg aagagaatca atagatagtt
     7441 ctagctggtt gccaaagctg acttgtagat ttgttatatt tactgctaaa ttttccatat
     7501 gttttacact cctatttcat gtaaaacaac aattatcgat actaaaaaag agcagaaaaa
     7561 tctgtttgca aaaaaactgc atcacatctt ttcctactct gaacgaaata actatgacta
     7621 aatcaccaaa tctgtcatag ctttcaccta ttctactgtc gttcgaaaaa atggacatac
     7681 tccatccatg aaattgttgt ttatacatgt cttttgaatt tatttagttg ttattcaaaa
     7741 gttcgaaacg taatttcatc gatgcagtca tttttccgac ctcctttttt attaggttac
     7801 cacacacgat tcaaaaaaag caatagctac tgaatagtaa ctgaaaacct tttattcgaa
     7861 cgtagtgatc ttttctggtg ctaaacgaac agactcgtaa ccttcttcta ccgcttttcc
     7921 tattgataaa ataataactg gaacataacg gtctttatcc aaaccaaatg cctctgccaa
     7981 ctgatctgct tcgactccac caattggatt tgtttcatag ccatgatcac gcgcaactaa
     8041 catgaattgc atagccgcta agctagaatc gactttgact acatcgttca tttcttctct
     8101 tgaaaaactc ttgtaataag gaatgatcgc agctaattgt tgatctctga cttcttttgg
     8161 catcttccct gattcgtaag cttggttata gatttcttcg ccatattcgt aacattgcat
     8221 atcgccaaaa atcatgacca ttgcagaaga agtatcattt tgtctcgtat tgaaacggat
     8281 caaaggacgc aagatttctt ttgcttcctc gctttcagcg accacaaagc gccatggttg
     8341 catattgaca gaagaaggtg cagtcgtcgc ttctttgatc atttccagca tttcttcgtg
     8401 tgggattttg tatgtttcat catatacgcg aacggatctt ctaccagtca taattgttga
     8461 aaaatcattt ttttgttcca ttattattaa cctctttcgt ttctgttgtt atattgcaaa
     8521 gttgggtatt caatttatct aaaagtgtta gaaattgttt catttctaca tcgctgaaag
     8581 ccggggatag cacggaacct gtatctttat ggtccatttc acaatgctcg attttcttct
     8641 gggctttttc tgttagcgct acatacactt cccggttgtt ctgaggattt ctctgacgcg
     8701 aaacatagcc atttgcttcc aataccttca gatgacgagt aactgctcct tgatcaatct
     8761 gcaacttttc ttgcaaactg ctttgtagca atggattttc ttctttcagt ctcatcatga
     8821 tctcgtatct agtaagactt ataccgatat tttcttcaaa gatctggacg attttttgat
     8881 cggctaattt gatttgatat aaaaggtgac taatctcttc taaagacata atttcctcct
     8941 ttctaataat tgactcgtca acgattgact tgtcaataat agaataaaaa aagaaaaaag
     9001 tcaaagaatc agctgtgtaa taaaaaaaac gctctcaata gaaaagtata gagaaaagaa
     9061 attttttgta gaatgaacgg aaaaaatgat aaagaaagca caacgctaaa accaagatta
     9121 ttgattctta tcaaactata gaagatagct cttctgcagt agaaaatttt acaccgccaa
     9181 aaatttccga aatcgccttt tgcaagaatc ccgtcgaata ttagaaattg gatcagtatg
     9241 cttaaacagt aataaaaaag ttatgacaag atgttgtcac aacctttttt gagtaaacgg
     9301 tgttcaaaaa cttgcatctg cggtaataag ttcaaccaaa caaaaaacat gaaaatctgt
     9361 ttttgtttgg ctgttcttac tacttgatgc agggcagctt tttcactacc tctttttaat
     9421 aaccataaaa tcctattcct tcatatctcc agcccatcgg tgttagccaa tctctttcct
     9481 tagcactcaa ggtatagtga tgggtaccag ttttttcatt tggattaaat agtcgataaa
     9541 ttggcttgga accaccagaa taaaaactta gaccttcata acgccaaccg atttttgtta
     9601 aagagttgat ctcagattgt gccattgtgt aatgatggtc tccactgtaa ggattgtata
     9661 aacggtagac aggttgtccg ctgtttggtg cttgccaagc aatcccttca tattcccagc
     9721 ctacagttgg cagataattt ttttctccgg cattcaatgt atataaatgt tctcctgtat
     9781 gacggttata cagacgatac atcggagaag ttgaagttgg tgctgtatca tactgtgtca
     9841 gattataggt ttcgattaca ttgttcaatg tagaaccata attaggcgca gtggcataac
     9901 gacctgttag ccaagccgta gcatctttgt aagagttcgt attgcttttc catgcaccgg
     9961 aatagtagta aacgcctggc tgaaaagaag tcgtcttcaa taccgttacg ttatcttgca
    10021 atgattcttt gtacgaagga tattttctaa aagcggcatt caccgtaatc cattgcccat
    10081 tcacatattc cgaagtaggc atcgtaacag attggccatt atagcttcct ttaatcccaa
    10141 atagattgta attaggggca gaagccaaag tactgttccc ccagccactt tcaacgatgg
    10201 cttgagcaat catgactgat gcatataagt cattggcact tgccaaactt tgtgcagttg
    10261 aggcaatact atcaatgaac gcttgttgag aattagcaga tgagcgagca taaaaagcat
    10321 agccagtata gttttcgaat tcagacaaat ctgcttgatg ggtatgtgtc aatgtttgcc
    10381 catctgtcaa ttggttcgtg atttcttctg cattctctgg attgttctga gcttctgctg
    10441 atgtactgtc ttcggtttgt gtttgttctg ttgtttcagt tgttggttct tcggttgaaa
    10501 ttgtattttc tgagacagta gctgactgca aagtactctc tgtatcgttt tcaattcctg
    10561 tttctgaaga actcgatgtc tgagttgcga cagtttctgt cgattcttca gctagcactg
    10621 ttgtgttggc aactgctaga tttcctaaaa gaataacgct aaataataaa ttctttgctt
    10681 tcatcttttt tctcctttga attatgaaac ttttcctaaa taacagtgta actaaaaaag
    10741 taaaagcaag tcaatgtcca tatttaaata acatttgttt ctttttgata aatttgcttt
    10801 ttctacataa aaagacgtaa atgaagaata ttcttctcca ttcacgcctt aaattgatgg
    10861 tcgtttgaat aactatttaa caagtaattc tctttggata ttatccgtta tggattatac
    10921 ttcctgtgtc gtcggacgga taatcatgtt actgacagac atccggtctg gcgtttctat
    10981 cgcaaatgct acagcagaag ctatatcttc ggaggtcaat ccccattctt tttgcgcttc
    11041 atgaagttca agagcagcct ttttgtcgct gatggtttga tataattcag tttgaacagc
    11101 tcctggagag atgatcgtag atttgatgtt attttgccgt tgctcttgac gtaatccttc
    11161 catgattgca cggacagcaa attttgttcc gcaataaacg gcggaatcag gataaacaac
    11221 atgtcccgcc acagaatcag ttgaaatgat atgcccagat ttttgttttt ccatgattgg
    11281 aagtacggcg gaaattccat ttaacacacc catgatgttg atatcaagca tattttgcca
    11341 ttcatcacgg tgaccttcaa ctaatggagc ggttggcatg attcctgcat tattgtacaa
    11401 tacatcgatt cgaccgaact tttccatcgc taaatcgata actgcttgga cttgtgcaaa
    11461 atctcttaca tctgctgttt gaatatatag ctcagcttct ggtaaagatt cttttattgc
    11521 ttttaggcga tcttctcggc gtgcagctat aactagttta gccccttttt ctgctaatag
    11581 cttcgttgtt gcttcaccga ttccgcttga agcgcccatg atgacaatca ctttttctga
    11641 taaatcattc attctattct cctcctgttt tgaaaaatct cttgcttata ctcactatct
    11701 cttatttcat cttactgttc aaataatccg ttcgacaggt ttgacgattt tggcagggat
    11761 tcctgcaacg atggtattgt ctgggacatc tttcgtaaca gttgcattcg ctgcaacaat
    11821 ggcattctcg ccaatcgtta cgcctggcag aatagttgct cctgctccga tccaagcatt
    11881 tttcttgatg tggattggct ttactaacaa gcctcttcgt tcttttggat cggttagatg
    11941 gttcaccgtg atgaggttga cacgaggacc aatcaatact gaatcttcta aacagatgcc
    12001 acctaagtca acgaacatca cttgacgatt gataaagaca tttttcccta tactaatatg
    12061 tttgccgaaa tctgtataaa aaggcagcag aacagtcacc gtatcatcta ctttgtcact
    12121 aatgatattt tctagccgtt tgcggatatc tgctggttca tggtacccag tattcagcgc
    12181 tgctaaaggc tcttcgtttt cagcttggat ctgatggatc acttcaaaga gcggatccgt
    12241 tactcgaatc tctttattga cgatttgttt ttcgatgagg cttgtcattt tgctccctcc
    12301 ttttatcctt actttataac ggacggctta tcatgtctaa tacttataaa acatacttaa
    12361 ctattccttt taactatact tagtggtatg cttttttaaa aacaggaggg aaaaaatgga
    12421 tattcgtata ctgacgtatt ttttagcagt agctagagaa aaaaatatca gccgagcagc
    12481 aaaatctctg catatcacgc aacctactct ctcaaaacaa ctgaaagatt tagaagaaga
    12541 acttggtgtg acacttttca ctcggggctc ccgagagata cagttgacgg attctggtcg
    12601 atatctttat tcaaaaggaa aagaaatctt gtcgttggta gaaaaaacgg cgaacaattt
    12661 aacaaatgac gcaatcatct ctggagaaat ctacattggc ggaggcgaaa ctaaagcaat
    12721 ctctttcatt tctgatagcc ttcattcgtt gatcaaggcg catcctgata tccagtttca
    12781 tttatatagc gggaatgcag atgatatcat agacaaactg gatagcggat tacttgattt
    12841 tggactcgta atcgaaccga caaacaaaca aaaataccag tcatttcgac tgccagccca
    12901 agatcgttgg ggacttttga tacatcgaaa tcatccttta gcaaagcaaa ccgtcatcca
    12961 tccgcaggaa ctgaaaactg ttcctttgat catttcccgg caaacgtcag tggacagtca
    13021 actagctgaa tggctaggtt tcagcataga tcagttatcg atcgtcggaa catataactt
    13081 attgtacaat gcttctttga tggtcgataa cgggactttt ggtgcattag ctctagatgg
    13141 gatcatcaat acttatggga cggaaaaaat attcatccca ctcgatccgc cattacaagc
    13201 ttcactgaat ctcatttgga aaaaagacca gcctctttct cctgctgctc gtaaattttt
    13261 agatgtccta acggagaaat acagttaagt agagtttttt tccttcgctt tcagcttttt
    13321 aacaacgaat aagataagca aaagatccaa cgattaaata acataagttt cttaatttat
    13381 ttcttccttt tatttctgat aattccagta aattactata attataaaaa aagaattgac
    13441 aaaacataac tgtctatata aaattgatta cgttaacaat aattcgtttc atgtgactag
    13501 atcatactag gcatgaaaaa tctctgagga acatggtttc cctaggaggt ttttcgtgtc
    13561 tatttttgtt tggccgcaat caactggtct agcaaacgtg aaaaccaaat cttcaaaaag
    13621 ggtgcgcgca tgaaaatcaa aaagatcttt aatcaaaatg cagtattggt agatgacggt
    13681 ggacaggaaa aagtagctat tggaaaaggg attggatttg acagaaaaag aaatgatttg
    13741 atttttgatc gtgatgtcga acggatattt gtcatggaac cagaaggtca aatcaagttg
    13801 caaaatttac ttaatcagat tgatgaacgc tttttatttg cagcagaaac gatcattgat
    13861 catgcggaaa ctgttttgat ggaaaagttg aatgaacatg tattgatcgc tttgaccgac
    13921 cacattgctt tttctgcgga gaacattaat aacggtatcg tcatccgcaa caaactttta
    13981 agagaaatcg aagtgcttta cagtgatgaa ttcagtattg ctcaatgggc agtagagtat
    14041 ctgacaaaag agcttgggat tccatatact tatgacgaag caggatatat cgcaatccat
    14101 attcacagcg gacgaagcgg tcggaacaat aaccatcgca gtatccgtga agtgacgatt
    14161 gtatctgaca tcatccattt agtggaatca gaattggaaa cagatatcca ttcagaacca
    14221 ttttcactga attattcaag actggtgaat catttacgac tgcttctaca acgaacccat
    14281 gcgcaacaat atgctgtttt agacacggaa atcgtagaaa tggtcaaacg aaaataccca
    14341 gaaagctata agatttctaa aaaaatccgt gtgttgctga ccaaagaata ccagctcgcg
    14401 atcacgaaag aagaactggg ttacttggcc atccatatcg agcgtttaag aagcgcaatc
    14461 gtacaaacaa atgttaacaa tcatgaggag gaaaaaaatt gaaagcatat ctacaacgaa
    14521 tgggacgttc ccttcaactt ccagttgcgg tcttacctgc tgctgcttta ctagtaggga
    14581 tcggaaactg gtgggcatct tatagtaacg acattgtcgc tcatttttta caagctggcg
    14641 gtaacgcagt tttaggtcaa ttacctttgc tgtttgccgt tgggttagca ctagggatgt
    14701 cagtggacaa atcaggtgct gctgccttgg caggtttggt cgcatttgaa gttccagtaa
    14761 atgttttaaa aactgattca gtagctactt tattgaatat caaaccagaa tcagttgatc
    14821 catcttttgc tcagatcagt aacgtattta ttggtattat tgccggattg attgcggcag
    14881 ctctttataa tcgtttccac gaagcaaaat taccgatggc tttatctttc tttagcggca
    14941 agcgtctagt gccaatcttg gctgcctttg tcatgttgct gatcagtgca gtgctactgc
    15001 tcgtatggcc tccggtttac tctgctttag tttcttttgc taaattcatt ttgagtttag
    15061 gctggggtgg tgcaggtctt tatggcttct tcaaccgttt actgatccct acaggattgc
    15121 atcaagcaat gaactccgtg ttctggtttg acgtagcagg aatcaatgat attggtaaat
    15181 ttttggcttc tgaaggaaca aaaggcgtaa caggaatgta tcaagctgga tttttcccaa
    15241 tcatgatgtt cgggcttcca gcaggagcat atgctattta cagaaatgca cgtccagaaa
    15301 acaaagcaaa aacagcttcc ttgatgctag cagcagcttt tgcttcattc tttactggtg
    15361 tgacagaacc acttgaattt tcatttatgt tcgttgcttg gccattatat gtcttgcatg
    15421 caattttcac tgggatttct ttagcagtga gtgcattctt ccattggaca gcaggatttg
    15481 cctttagtgc cggatttgtc gattacttcc tatctttgaa aaatccgatt gcgaatcagc
    15541 cattgatgtt gatcgtccaa ggattagtaa cagcagtgat ttactacttt ggctttaact
    15601 ttgctatcaa aaaattccat ctgatgactc ctggccgcga ggaagcagat ctttcagatg
    15661 acgatactgc aacgactaat tcagacaata aatatgcggc acaagcaagc aaaatctacg
    15721 ctgcattagg cggagccgac aatgtaactt cgatcgataa ctgtactaca cgccttcgtt
    15781 tgcaagtcaa agatacaggc ctgatcgatc aaaataaaat caaagccact ggcgtacctg
    15841 gtatgaagat catcgacgga aaaaatgcgc aagtcatcgt aggtacagaa gtacaattcg
    15901 ttgcagatga gatggcaaaa ctacatggtg gtgctgcagc acgacctgca caaacaaaca
    15961 ccgtggttaa aacagaaaca tttgccggtg aaacgatcga acaagatatt tacgctatcg
    16021 caaatgggaa actgattcca atcactgaag tttcagatga tgtattcgcg gaaaaaatga
    16081 tgggtgacgg ctatgctgta ttaccagaaa atggagaaat cttctctcca atcgctggta
    16141 cgatcacaaa cattttccca acgaaacatg ctgtcggaat ccaaacagat gctggtatcg
    16201 aagtcttgct tcatatgggc atcaacactg ttgacttaaa aggcgaacca ttcactttat
    16261 atgtagaaga aggtcaaaaa gtcgcaagag gtcaattgat tgctttagtt gatcttgccg
    16321 ctattcaatc tgcaggcaaa aacacggata tggtcgttgt attcacaaat ggcgacaaag
    16381 tccaaagctt agaaatcgaa cccgcacgtg atgtaaaagc aaatgataaa atcggctctg
    16441 tttccaacaa agcctgagcc atcgtcttcc ccagactttt ttatcgataa acaaacagga
    16501 gctgagacaa aaatcttgtc tcagctcctt tttccgaata aaaacggcgt tctcctgcca
    16561 actccgcggt aataagtcca gctaaacgaa acatatgaag agctattttt tatttgactc
    16621 ttcttattac ttgatgcaga acagcttttt cacaaaattt ttcttcatac tcaaaactaa
    16681 tcggcttttt cacaacctct ttatacatta ttcctgatcc aatatttctt ttaatgctgc
    16741 ttcaagagaa gttaatggtt ttcccaatac tttttctaaa tcatcagagg aaatagctaa
    16801 ctgatccttt ttaatatcat gttggatcgc taaaaacatg tcagctgctt cttgagggaa
    16861 tccggcttct tttagatttt cgacaaacgc tgcatcactt gcttcaataa tttctaactc
    16921 tttacctgtc gcacgctcta atgccttggc taattcttca tagtcagtta gattgccgga
    16981 taattctaag atttctggag agtcactttc tactagtact tttgctgcag cttctgcata
    17041 ttcacgtttc aatgcccaac ctgtttttcc atttcctgca gcataaacga attgaccagt
    17101 ttttaaagcc tgcccaatga taggtaattc attctctaca taccaattat tccgtaaaaa
    17161 tgtatgggaa atacctgaat ccttaatcat tttttcagtg aattggtgat ctggcgctaa
    17221 tacactgatc gagtgatcgg catctgcaaa acttgtataa gcgatataag ataccccagc
    17281 tgcttgtgct gccttcacta cattcttatg ttcggcttga cgatttcctg gaacgccaga
    17341 gacaaaaagc aatcgatcta ttcctttcaa cgcttctttc attgagtcta aatcatcata
    17401 atcaccaata cgtatattaa atccagcttc tttcagtgct tctccttttt cttcactacg
    17461 tactagtaca tagatttcat tttgtggaac catctttttt aagaattcca gcgcataacc
    17521 accaaaacct ccagttgctc ctgttactaa ataatgcata tttgccactc cctattcttt
    17581 ttatttgtta agatttacag ctgtacattt ataaattaca cctttatcat aaagagtgtg
    17641 gtaagataag tcaagcaaaa aaaatgaaag gatgacaact gatgaagtat tctctgcaat
    17701 ttagtgatgc aatccatgta cttgcctata ttgaaatgtt tcaagatacc cgatcacttt
    17761 ctagtgagat gattgccagt agtatcgaaa caaatgcagc aaatgtacgt aaaatcatga
    17821 gtcagctcaa aaaatccggt ttgatcttta caaaaaccgg aaaaccgcag ccttcccttg
    17881 ccaaaagtcc agatcagatt tctttactag atgtctatcg cagcatcgaa ggtaatacac
    17941 agcttatcca agtcgatcca aaaacaaatc caaattgcgt cgttggtgcg aatatccaag
    18001 aagctttaga agaaagctac gctcggctgc agaaaaaggc agaagaagaa atggctaaga
    18061 tcacacttga tagtctgata cataaaatag ccgttttgga aaaagaaaaa agaccagaaa
    18121 acacaggcct cgtagagaga tttttataat tctctctact gtgcctgcat ttttctgatc
    18181 ggaatcgtct acataaattc ttttattctc cgggggacaa aagccttgtc atgccccttt
    18241 ttcctctcta atgctcaggt caaagcgctg aagtcatgac gtcttgataa acggtgttca
    18301 agtagctgac cctcggcatt agttctgaaa aatgcaaaat atggaaagct atttcccatt
    18361 tttcctctct aatgctcagg tcaaagcgct actttcacga cctctatatt atcatggttt
    18421 attgactacc ggtatccaga tttctcccat atatttcccg ttggtaactc ccatttcgac
    18481 tacagcattt ggacccccaa cataggcgta atcttctact tctcttaaaa catgcccaaa
    18541 ggccgtattc gtcaaatcat ttcttaattc ttcaggcgat tctgctaaag cgcttacaac
    18601 aagatacgtt ccttttggga atgggatgat tcgcgtagtg tttggaacat cctgactcgt
    18661 ttgaacaccg acatagtaca tcattttccc gttatagggt tcgttgactg taaagaaata
    18721 atgattttca gcagcttgtt tcaatgcatc tattgttcca ttaccgataa ctttttgctc
    18781 aaatgcttgt ttctctgcag ccaaaccgac aaaatccgta ttgctactct tcaattccac
    18841 accaataccc gtcaccgtaa aatctggttt ttcttcgatc ctatactttg ccatttctcc
    18901 caccctttct attatattaa gttcatctta cataaaatta aagggaaatg acacgattac
    18961 gctctttttt ctagggatat ttttattttt taactatttt tttgctttca ctgcaatcca
    19021 aatctcactg taaacattac tgaaatcctc tccccatttt gtaaaagaga tctcgggagc
    19081 ttcgactaac ccataatctg aagaaggaag ccattctgca tagattttcc cccaagtatc
    19141 ttgtaaagtt tggggaaatg gccctttatt aggaaaaatg gcccaagtgt gttcttctat
    19201 agcaagcatt tctaaatcag cgtacggatt ttcttttgtc gtagcaaagc caatcatgtg
    19261 catcaaactg cccttttctt ccaagcggtc tccatcaaat ccataagaaa cgttgattac
    19321 ttgatttgga tataaatctc ctaacttatg catctcttcc ctttgagtct ccgtgatcgt
    19381 ctgcgctaac ttttgtattt cttgattgac tccttcaaat ttgacaggaa cttttttcgc
    19441 cacacctgcc acataaaact gttctttctt ttcgatacga aattccatcg attttcctcc
    19501 tctgacatca ataaaaaatg tcaagcgtgg aaaagatttt tgtatgccgt atctgctgat
    19561 ttctgaaggc agatatcctg accaatcacg aaaagctctt gaaaaacctt cgactgtttc
    19621 atatccatat ctaaacgccg tttctgtcac tcttgatcca tctagaagat cgcgattagc
    19681 caaagccaat ctacggtttt tgatatagtc cattagcgac atccctgcaa taaacgtaaa
    19741 gatgcgtttt aattgatatt cagagattcc tgtattttcg gctattattt tataggaaag
    19801 tccttctgtc aaatgttctt cgatgtaatc gatcaattgg ttcagatctt taagcatatt
    19861 tatcttcctc cttgattgcc tttttatcat attatcaaag aaagtcaatt acttgatgaa
    19921 tcaggaacca atttagtcag atgtagaaaa tgttgtatgg atctaaagaa aattctcaag
    19981 cagcgtttcg taacggataa ttggtattat ctgcctatct atgttttacc tttagaaaaa
    20041 taaattttgg ggaagacaga ttctaagtaa acaaagcggt aaaaatatct tttattccct
    20101 tagttaatcc gcttactacc tactgtacaa agcaaattgt tgttcaaact gctgatcaga
    20161 acgctgcttg gatttttctt t
//
LOCUS       DAYYGO010000052        19507 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000052 DAYYGO010000000
VERSION     DAYYGO010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.053"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(460..1089)
                     /gene="udk"
                     /locus_tag="ACHA5T_001518"
     CDS             complement(460..1089)
                     /gene="udk"
                     /locus_tag="ACHA5T_001518"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFM8887096.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1173..1868)
                     /locus_tag="ACHA5T_001519"
     CDS             complement(1173..1868)
                     /locus_tag="ACHA5T_001519"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFM8887097.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1881..2597)
                     /locus_tag="ACHA5T_001520"
     CDS             complement(1881..2597)
                     /locus_tag="ACHA5T_001520"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFM8887098.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2927..3532
                     /locus_tag="ACHA5T_001521"
     CDS             2927..3532
                     /locus_tag="ACHA5T_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFM8887099.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3857..4180
                     /locus_tag="ACHA5T_001522"
     CDS             3857..4180
                     /locus_tag="ACHA5T_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFM8887100.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4362..5732
                     /locus_tag="ACHA5T_001523"
     CDS             4362..5732
                     /locus_tag="ACHA5T_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFM8887101.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5862..7163)
                     /locus_tag="ACHA5T_001524"
     CDS             complement(5862..7163)
                     /locus_tag="ACHA5T_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFM8887102.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7393..9384)
                     /locus_tag="ACHA5T_001525"
     CDS             complement(7393..9384)
                     /locus_tag="ACHA5T_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFM8887103.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDKVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9528..9794)
                     /locus_tag="ACHA5T_001526"
     CDS             complement(9528..9794)
                     /locus_tag="ACHA5T_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8887104.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10304..12430)
                     /gene="pnp"
                     /locus_tag="ACHA5T_001527"
     CDS             complement(10304..12430)
                     /gene="pnp"
                     /locus_tag="ACHA5T_001527"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFM8887105.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12618..12887)
                     /gene="rpsO"
                     /locus_tag="ACHA5T_001528"
     CDS             complement(12618..12887)
                     /gene="rpsO"
                     /locus_tag="ACHA5T_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFM8887106.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13138..13701
                     /gene="def"
                     /locus_tag="ACHA5T_001529"
     CDS             13138..13701
                     /gene="def"
                     /locus_tag="ACHA5T_001529"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFM8887107.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(13954..14475)
                     /locus_tag="ACHA5T_001530"
     CDS             complement(13954..14475)
                     /locus_tag="ACHA5T_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFM8887108.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14478..15320)
                     /locus_tag="ACHA5T_001531"
     CDS             complement(14478..15320)
                     /locus_tag="ACHA5T_001531"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFM8887109.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15313..16038)
                     /locus_tag="ACHA5T_001532"
     CDS             complement(15313..16038)
                     /locus_tag="ACHA5T_001532"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFM8887110.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16214..16510
                     /locus_tag="ACHA5T_001533"
     CDS             16214..16510
                     /locus_tag="ACHA5T_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887111.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      16766..16940
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17078..18670
                     /locus_tag="ACHA5T_001534"
     CDS             17078..18670
                     /locus_tag="ACHA5T_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM8887112.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18683..19423
                     /locus_tag="ACHA5T_001535"
     CDS             18683..19423
                     /locus_tag="ACHA5T_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8887113.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
BASE COUNT         5580 a         4001 c         3702 g         6224 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaaa cagttggctg gtgactgttc aaaaagcaga atccttgctt
      421 ttcaaacggt cgcagtccgt tcaactgttt cgctgcgcac tatttatggt ttaagaaact
      481 agctactttt gttgtaatca aatcaatagc aacgtggttt tctccgcctt ctggaacaat
      541 gatatccgca tagcgcttcg ttggttcgat aaattgatga tacatcggtt taacaactgt
      601 caaatattgt tcgatcacgg agtccaatgt gcgtccccgt tcttccatat cccgtttgat
      661 acgacgaatg atacgaatgt catcatctgt atctacgtat actttgatat ccatcaagtc
      721 acgcagacgt tcgtcttcta aaatcaggat tccttctaga atgatcactt cttttggttc
      781 ctgaatgatc gttgcctgac ttcttgtatg cgctacatag tcatacacgg gtttttcaat
      841 ggctttatag ttcagtaagt cgcctacatg ctgaatcagc agatcattat caaaggcaaa
      901 tgggtgatcg taatttgtgt tcaatcgttc ttcaaaactc aaatggctct gatccttgta
      961 ataagaatct tgttcaagca tcatgatgga atggtccggg aagttgttga aaatcgcacg
     1021 gctgacactt gtctttccgc ttccggatcc acccgtcaca ccaatgataa ttggcttgct
     1081 ttttgtcatt gactaaaacc tctttcgtat ccgttttttc attctaatct attatagcga
     1141 aaaaacacga aaatgctata tttttctgaa aattaatcct tttcaatttc caataatgaa
     1201 taaagttcct ctaatgaatc gattcgataa gtaggctgga tttcttcgat ttctggtaat
     1261 ttatttctat tgaaccaaat cgtatcgatc cctgccatat ttcctccttt gatatcagaa
     1321 gtcaaagaat caccaatgat cacagtttgc tttttatcaa aattaggaat gtgggaaaag
     1381 acatgatcaa aaaattcttt catcggcttt tgcgctccga cagcttctga gacaaagata
     1441 tccttgaagt aatcaagcat tcctgcatct gttaatcgcc gatgttgcgt gtgcgacaca
     1501 ccgttcgtca caatataaag atcagcatgc ggatggacct ttttcaggat ttctagactt
     1561 cctggaacaa gatcatgacc ttggttcaag tagtggcggt agcgtttttc catttgatca
     1621 ctatctactg ttttcccaaa ttgctggaaa agtaacccaa aacgactgcc tattatttct
     1681 tctcgtgata gtctgccttt ttcaaactcc tgccataaat gatgattcaa agccttatag
     1741 ctggtttcga tttctggtgt caatgtgatt ccttgttctg caaacaaagc acttaaagca
     1801 ttggcttcag ttaattgaaa atctaacaat gtatcatcta catcgaacaa taaagtttta
     1861 tatttcatct ttttgacctc ctatttaaaa taaatactta actttttcat ctctgctaat
     1921 gtcaacgatc gatactcgcc tactcctagc tgttcatcta attccaatgg tcccatgctt
     1981 agccgtttca atgcggtcac tttctttcct tgggctaaga acatcttttt gacttgatgg
     2041 aattttcctt cttggatcgt caataaaacc gtactttggt gcttgtctgc tttgagtatt
     2101 tttaaatcag ccggcttaca ttgataccca tcagcgaata cgattccatt ttcaaatgct
     2161 gttacgtctg ccggtgtcac cctttcgttc acaactactt gataagtctt tgaaaccttt
     2221 ttttctggaa gtagcatttc atatcccaat tgtccattcg aagtgatcag caatagtcct
     2281 gttgtatctc tatctagtct tcccactgga tataatggtt ggaaacgatc ggcacttgca
     2341 atcagatcga ttactgtcgt cttttctgga tctgatacag ctgtaacagc tttttcaggt
     2401 ttattgagaa gataatagga ctcatttgtt tctaagtgtt cacctgctac ttcgatttga
     2461 tgcagtaaac tatctacatt tcgattttgt cgaaattcga ctgtgccatc tatcatcact
     2521 tgcttcaaag caaacagccg cttcatttcc ttgcgtgtcg tttctaattt ttcttctatt
     2581 aatttatcta aacgcatgct gtttcctttc ttattactac taattaatta taccttgaat
     2641 attcttgctt gcaaatacat catcatagtt attccttgac ttaattaaca gttcacgtta
     2701 cggttaaata gtaaagtgaa ttggatttct catacacagc gtaatgacct ggtggagaaa
     2761 agttgaatcc tgactttcta aggggagatg cgcgcatctc tccttttgcg cttttttaag
     2821 cgccctgcct tcgatgatgt gcacgagatt agcctttaga attcacttaa ataaaatctg
     2881 tccgttacca aagcagacag tcacatttta aggaggaatt ttggttatgt cagtatcatt
     2941 agaagtaagt aaaagagaag tacgtccccg ttcattaaga aacaaacttc gtcacgaagg
     3001 gaaagttcca gctgttgttt atgggtataa agtagaaagt acccctatct cattcgatgg
     3061 tttgacacta agcaaattgt tgcgtgaaca tggagcgaac acagtcatca cgatggacat
     3121 cgatggacaa aaagtcaata cattggttca caagatccaa acgaacactt ttaccaatca
     3181 tttcgaacat gcagaatttc tatctgtaaa catgtcggaa gaaacagaag tcgaaacaga
     3241 agtcgttctt gttggcgaat cagtgggtgc taaatctggt ggagtgttag ctcaaaactt
     3301 gtatacagta gtcgtatctg ctacaccaga caaattgcct gaacgaattg aagttgacgt
     3361 gacaaacttg gaattaggtg attctattac agtagccgat cttccaaaaa acgacgatta
     3421 caaattcgtg accgacagtg aagagcaaat cgctgctgta gtcgctcaaa gtgaagaaga
     3481 aagcacaggt gaaggtggcg aaccagaagt gatcggttca acagaagaat aatactgaaa
     3541 caatcaggtg gtgttagaag cggcgggtca gcagctttta acacgccttt tttatttcta
     3601 atcgtttatt ttcatatggg gcttccactt ttgtaaatgg ctttacaaac gaaaaatcct
     3661 aatttaccaa gcttttcctc gcatactttt atcgtacctt tataaaaatt tatactttga
     3721 aaaaaagtat tcaattattg ttgcattttt cctgattctt tattagaatg aaccctcgtt
     3781 gaagtaagta tttctagttt atcctaggat taattataaa aataaaacat tcgttgattc
     3841 gaaggaggtt aaacagatgt ttgaaagttt tgatagcagc cgcaaccgtt acgcttcagt
     3901 tggtgtcgta tcaagtttgc cagatgaatt gattgacagt atatggttta ttattgacct
     3961 agatcttaag ggagtcattc cattagaaaa tatattaacc tttgatttga tcgaaaatcg
     4021 tggaaaagtg acgatgcatt tttctcaagc aggaagcaat gtggaaatgg atattgattt
     4081 gccgtttgcc tattcgaacc aatttcctgc tcaagtatac gcctatgatg acggtacgag
     4141 agaaacgatc cttttaccaa gtgagattag acaatattaa ggtgatagat aaacgtggat
     4201 gtcccttatt ttacagacca ggccaagttg ttgattcttt gactcaattc ttggcttctt
     4261 tttatggagt ttcataaatt atcattgatt tctaattggt attctcagga taaacgctat
     4321 atactactaa ctaaatttta aagatataag gagtttaaaa gatgcgcgat ttaacaaaag
     4381 gaagccccgc taaactgatt ttgatgttca ctgttccttt actgatcgga aatgtttttc
     4441 agcagttcta caatatgatc gatatgatca tcgtgggtca gacgctaggt aaaaatgcgc
     4501 tggcagcggt tggtgcaact ggaagcctga catttttgat cattggcttt gcccaaggtt
     4561 taacagctgg tttagccatt attaccgcac aacgttacgg tgcaaaagat tatcggggtc
     4621 tgaagaaaag ttttgctgct agtgttgtca ttagtcttgt cgtcactgtg attttaactg
     4681 tcctaagtct cctgtttatt cgtccgatgc ttcaattgat gcaaactcct cctgaaatac
     4741 ttgatcaagc acaaacattt atttcaatta ttttattagg gatctttgca tcagtcagtt
     4801 tcaatttgct ttctaatgtc attcgcgcac ttggtgacag ccgcacacca ctattctttc
     4861 tgatcattgc ggtcatcatc aacgtcgtac ttgatttgat ctttattatc tattttggca
     4921 tgggtgtgga aggtgcagct attgctacag taatcgcaca agtttcttcc agcgtactgt
     4981 gtctagtgta tatcaagaaa aaaatgcctt tgttacagtt acgcaaaaaa gatttcaaat
     5041 ttgaaaaaga agagtttgct gtacacctaa atgctgcttt gccaatggcg tttcaatctt
     5101 cgatcatcgc tgttggtgct atcgtgttac aagcagcact aaatagtcta gggacagatg
     5161 tcgtagctgc tcaagccgct gctggtcgta tcgaccaatt tgcaaatcag ccgatgatgt
     5221 catttggtat tgcaatggca actttcactg cacaaaacta tggtgcgaaa gaatatggcc
     5281 gaatcttgca aggggtacgt caaactttac tgatgagtgt cggatttagt ctcttagcag
     5341 gtgctatcgt cattttcttc ggtcatttct ttgttcaatt gtttgttagc ccatcagaaa
     5401 cacgtgtatt tgaattagca caaacctact tcaatatcaa tgggtctctt tactggatct
     5461 tagcagtcct gttcatttta cgctacacac ttcaagggct tggacaaagc aagatcccta
     5521 cgatcgccgg tatgatggaa ttgcttatgc gttcttttgc agctatcgtt cttacaggaa
     5581 tgtggggata cccaggagca gcagcagctt ctcctttagc ttggatcggt tctgttgccg
     5641 tgttgcttta ctcttatatg cgttcgatga aacaattgaa acacatgggt gaagaggaac
     5701 atttcacatt agaacatgca gaagcaagat agccttgatt aaaagaaatc gttgcggcta
     5761 aaaatgaaaa cttttattga aaatatcttc ttcgtttacc gaaaacaggt tttttagata
     5821 acaaaaaggc tgtgacaaaa aatgtcacag ccttttttct tttattctat ttttctttcc
     5881 gtggactcta ctttgaaata atcgatatgt gcatgctggt attctaatac cgtgaaacgg
     5941 aatgatgcaa tctcaatgat tgttcctgct tttatttctt tatatcggct agaagcaaag
     6001 cccgcaagtg tagtaaaatt cgtttcttca aactctggaa tctctacatg aaaataacgc
     6061 tggaaatcat ctaatgttag tttgccggag atgatataag agccatctga ctgttccgct
     6121 atcaatgctt cgtccaaatc tgtctcatct cggatttccc cgacgatttc ttctaaaata
     6181 tcttcaatcg tcactaagcc gcttgtccct ccgtattcat cttttaagat agcaatatgc
     6241 tttccctttt ttcgcatgat cgtcaataat ttttttatag ggatcgtttc agttgttacg
     6301 atcggctctt caatcaactg ttctactgtc agctggtcat ctttataaga ttgcttgatc
     6361 aaatctggca aggtgacata acctaaaaca ttgtcttttg attctaagat aaccggatag
     6421 cgagtatgtc cttgttcaat cgaagctttt atcgcttctt tgacggtttc tgaagcttcg
     6481 aatacttgca tggacgtacg gtctacttgt atatcttttg ccaaggtatc atcaaattca
     6541 aacacgtttt ccaaataatg atattcttct ttggtcaact ctcctttttc ataagaattc
     6601 aatgcgacta atgtcagctc ttcttgagac aatgtctctt ctccttcaga tacgagacgc
     6661 acacctaata gcttccctag ccctgcagct gaatgattca atacccaaat aaaaggaaac
     6721 attactttat aaaaataatg aagtggttta actacgaaca agacgacctt ctccgtattg
     6781 acgatactat agctcttagg aagcagttcg ccaataacca catcgatata cgtaactaaa
     6841 ataaatccca gcgccacaga gattgtactg ctaatagccg aactaaaagg tagtttaccg
     6901 atcactggtt ctagtaaagc cgcgacagat ccttcaccga cccacccgat gatcaaaccg
     6961 gttagcgtaa ttcctaattg actagctgat aaatagttat ctaaatggtg tgtaacatga
     7021 agagctagct tggcgttctt tacattttcc ttttctagtt gttccaatct tgatcgtctg
     7081 attttcacta aagcaaattc acttgctaca aaaaatgaag taattgcaat catcaaaaat
     7141 aaaataatga aattgataac catattctcc cgcctttctc tttttctatt gtaccctttt
     7201 tctacaaaaa gattgaaaat aatgcgtatt catatttagg aataaaaaag gagatgcggc
     7261 atttttcagc cacatctcct tatcacttca aaatcattgc gttacttgtt gctaaacagc
     7321 tttttcatga cctctgataa acggtgttca agtagctgac attcgacatt agttcaggtc
     7381 aaagcgctac tttcacaacc gctctttcaa ccatgttttc cgagtatttc caatgataag
     7441 cggaacagtt tcatgtttta cttcgctgta ctctagccca aaccatgttt ctggcgaggt
     7501 agcgattttt ttgattgcca gttttgcctg catgatttga cgttgccatt ttcctagagt
     7561 tgtcacattg gaaactactt tatcaatcag gacaaattgg aaatcgccaa cttctcgccc
     7621 tggtgtcaga gagtatcttt gtggctgttt tggcaaacgg ccttgtttca tcaaatcatg
     7681 gatgacttgg cgaacatata cgttgatttc ctgttggacc ctgaaaccta agaacagatt
     7741 cacttggaca ataaaatcag tgtccatcat atctacttga tattctttcg tatatggctc
     7801 atccgtcact tccacatgaa caaaccaata gactcgtgct cttttaggcc gtttatctaa
     7861 aatcgaatag atgaactgtc ggccaacttt atcttcagcc atatcaggaa ccaagaagac
     7921 cacatttgtc tgatagattg gtaaagaagt atcatcacgt aattctctaa gctgcgggat
     7981 ataatctctc aaagacacat cctcggttga cgattcgcga ataatgttcc cttgttccca
     8041 aataaacatc acgactaaga tgatcaaacc aatcagaacg gctacaaaac cgcctcggaa
     8101 gaatttgaca acacttgaga taaagaaaac gacttctatc gcaccgaaga aaaaggtaac
     8161 taaatatgca agccaacgag aatgcccatt ttggataaga taaaagtaaa gcagtgctgt
     8221 tgtcatcaac atcgtcacag tgatcgatag accgtaagca gcttccatat gatgagacgt
     8281 tcggaatgtg acaaccacta gagaacaggc gatccataaa atcagattga ctgctgggat
     8341 atacatttgc ccgatactgt tcccaggata catgatcttt aaccgaggca gcagtttcaa
     8401 cttgattgct tctgaaacta gcgtaaacga acctgtaatc aatgcttgcg aagcaatgac
     8461 tgcagcgact gttgcaaaag caacgccgat cacgatccaa ctttgaggca tcatttggaa
     8521 gaatggattc aggttcttga tttgctgtgc ttcagggttt tgataaacat tcaacaacca
     8581 cgcagcctgc ccaaaataat tgataaccaa acaaattttg acgtaaggcc agctcagacg
     8641 aatatttcgt ttgccgacat gtccaagatc ggaataaagc gcctctgcac ctgtcgtagc
     8701 caggaaaata tttccaagaa tgaacaagcc taatttgtta tctgcactaa aaagcagctg
     8761 gatagcataa taaggattca gtgcacggat aactgtccag tcttgactga agttcattaa
     8821 tcccattacg cctaaaaaag taaaccaagc gaacatgatt ggtccgaatg cttttccgac
     8881 aatatctgta ccaaagcgtt ggaccatgaa taaaacaaaa ataataacta aagtgataat
     8941 gacgatcacg ttctggttat taccgaaccg gtcaaaaaac tgcggaattc ctcgcaagcc
     9001 ttcaatcgca gtcgttaccg tcacagcagg ggtcaatacc ccatctgcta gtaaggcagc
     9061 tcctccgatc attgcaggaa tgatcaaata tttcccacct tttcggacaa gtgtgtagag
     9121 tgagaaaatt ccaccttcac catggttatc tgcattcaat gcaatcacga catatttgat
     9181 tgttgtcagt agagtcaatg tccaaaaaat caatgagact gatccgataa caaaatcagg
     9241 attgattcct gacaatccgc cattatcttc aacaatcgat ttcataacgt aaagcggact
     9301 tgtcccgatg tctccgtaga cgactcccat cgctacaagc aggccagcca ttgatatctt
     9361 tttggtattg tgcgttgttg ccacgttttt ccctcatttc ttgttatatg gatatccttt
     9421 aggtaagctt gaatgataga tgatattctt atccatgaca cccgctttcc taaattgctt
     9481 ctaattatcc aagtaacaca ctgaagtgtc aacttatttg aataaaatta ttctttcttt
     9541 ggacgttctc tgatacgtct tgccacaaat gaataaagag caaggcaaaa aattgcaccc
     9601 aataatgctg gtatcaaata aaaaccgccg agttccggtc ccagtggtcc tagtaaaaat
     9661 tcgccgagcc aactacctaa aaaaccgata atgatatttc cagatacgcc tcccgggact
     9721 tcctttccta aaattgcgcc agctatcaaa cctaatatac cgccgataaa taacgaccat
     9781 aaaaaattca gcatctcctt tccctccttt atccatattt ctcactttta ttttacgctt
     9841 tattcagaag gactccaaaa atatgcgcca agaaactcca gaaaaaaaga agttgtaaca
     9901 aaagtgttca gcttatttcc gtagaagctg cttctggaac accgtgaatc agaggttgtg
     9961 aaatcagcgg tttgacctga gtattagaga aacaaacgga aaaatagttc ctcatatttt
    10021 gccgtttttt gagctaatat cgagggacag ctgattgaac atcgtttatt ggagaggttg
    10081 taaaaaagct gttctgcatc aagtaataag aacagccaag taaaaacagc tcctaatgtt
    10141 tttaacttgg ctggacttat taccgcagat gcaagctttt gaacaccgtt tattcgaaaa
    10201 aaaaggactg tgacacggct tttgtcacag tccccttttt tcgaataatt attgtccccc
    10261 tgtcaccttc acgatcacaa gtacattatt cctatgaatt aaattatttt tcttcatttt
    10321 tcgttgaacc atcttcgttc aacattgctt ttcttgacag atttacgcgt ccttgtttat
    10381 cgatttcagt tactttcaca aggatctcat cacctagttt aacaacatct tcgactttat
    10441 tgacacgatc gtttgctaat tgggaaatat ggacaagtcc atcttttcct ttgatcaagt
    10501 tgacgaatgc accgaatttt tcgatacgga caactttccc tagataaact tcaccaactt
    10561 tgacttcttt ggtcaaatct tcgatgatct tgatagcttt tttgatcatt tctgcatcag
    10621 ctgaagcaat acttactttt ccatcttgat cgatatcgat tttcacacca gtttcatcga
    10681 tgattccgtt gatcgtatcg ccgcctttac cgataacatc tttgatttta gcaggttcga
    10741 tctggatcat ttcgattttt ggtgcatatt ggcttaattc ttcacgtgga gcagcaatcg
    10801 ttgaagtcaa ttcttctaag atttccatac gtgcttgttt tgcttgagtt aatgcttcag
    10861 ttaggatttg ttctgtgatt ccttgaatct tgatatccat ctgtaaagca gtgataccat
    10921 cttttgtacc ggcaacttta aagtccatat cgccaagatg atcttccagc ccttggatat
    10981 cagttaggat cgtatagttt tcaccatcag aaacaagtcc catcgcaata ccggcgactg
    11041 gcgctttgat tggtacacct gcatccatca atgctaatgt gccagcacag atacttgctt
    11101 gggaagaaga accatttgat tccaatactt ctgctactaa acggatcgta taaggaaatt
    11161 cttcttctga agggattact tgtgctaacg cacgttcacc taatgcgcca tgtccgattt
    11221 cccgacgccc tggagagcct gcacgtcctg tagaaccaac agaaaattga gggaaattgt
    11281 agtggtggat gaatcgtttg ctgacttcca cccctaaacc atcaatgatt tggtgctctc
    11341 ctagaggtgc gagtgtacaa gctgataaag cttgtgtttg tcctcgtgtg aacaaacctg
    11401 atccatgtac acgtggcaat aagctgacct ctgatgacaa tggacggatt tcatccaatt
    11461 tacgcccgtc aggacggatc ttatcgatgg tgatcaattc acgtacgaca tctttttcaa
    11521 gatcttctgt gatttgttta acttctttca acaagtgatc ttcatcttca tgacctgcaa
    11581 atttctcagc ataagcttcg cggatatcga tcttcacctg ctcgatatta tcttcgcgag
    11641 caagtttttc ttcagtcatt actgctgctt tcattgtagc gtaagaagca tcaaagattt
    11701 cttttttcaa tacttcatca acttgcagta aagtcacttc cattttttct ttccctactg
    11761 cttgaacgat ttcttcttgg aaagcaacta actctttgat agcgtcaaaa ccaaacagca
    11821 atgcaccaag catgtcttct tctgaaactt ctttcgcgcc gctttcaacc atattgatgg
    11881 cttgtttcgt tccggcaaca gtcaattcga tatctgtttg ttctgcttgt tctactgttg
    11941 gattcaagac gtactcgcca tttacacgac caacttctac gcctgcgatt gggccgtcaa
    12001 atgggatatc tgagattgat agtgctaaag aagaacctag cattgctgcc atagctggcg
    12061 agcagtctgt ttctacactc atcacgatgt tcgttacttg gacttcatta cggaagcctt
    12121 ctgcaaacat cgggcgaatt ggacggtcga tcaaacgggc agtcaatgtt gcttccgtac
    12181 ttggacggcc ttctcgtttg atgaaacctc cagggatttt ccctgctgca tacatttttt
    12241 cttcataatt gatcgttaat gggaaaaagt ctgtatcttt tgcttcttta gaggccacag
    12301 ctgcacttaa gactactgta tctccataac ggactaaaac ggctccgttt gcttgttttg
    12361 ctagctgacc gacttcgatt tgtaagggac ggccgcccca tgtcgttttg aatacttgtt
    12421 tttctgacat aacttctcct tttccttctc gtgaaagcaa gacgctacta aacaaatgaa
    12481 aactgccgaa agccgtcttt tgagcagctt tcatttggtt agtaggagtc catctagctt
    12541 gatcacggaa ttttacttaa ttgatatacg agtaaaaaaa gtgagattca tgtgaatccc
    12601 actctttttg actaaaatta acgacgcaag cctaaacgtt tgatcaattc gcggtaacgt
    12661 tgaacgtcag ttttacgtaa gtaagctaac aagttacggc ggtgaccaac ttttttcatt
    12721 aggccgcggt atgaatgatg gtcttttttg tgcacgcgtg catgttcatt caagtggttg
    12781 atttcttcag ttaatacagc gatttgtact tctggagaac cagtatcccc ttcatggcga
    12841 gcgtattcgt tgatgatttc attcttgcgt tcttttgaaa ttgccatttc tttcacctct
    12901 ttcgattatt cccctgcgac tgagtaaaac gttggtgatt cgcttaacca agtggcaggt
    12961 catgtgccta gagcacacag ctatacttta cctgaaaccg ggttgaaaat caagtagtta
    13021 atcattaaga aaaggtagtt ttcaaacaat cttactacta cttttccatt tttgtggtaa
    13081 actataatgc gaaaatagac ttgaaacaca gtcttttaag aaaggggtac atattccatg
    13141 attacaatgg atgatatcat tcgtgaagga aatccgacct tacgcgaagt tgcaaaagaa
    13201 gtttctcttc cattaagcga agaagatatc tctttaggaa aagagatgct tgagttctta
    13261 aaaaacagcc aagatccaat caaagctgaa gaactacact tacgaggcgg tgttggctta
    13321 gctgctcctc aattagatat ttctaaaaga atcattgctg ttcacgtacc aagtcctgac
    13381 ccagaggcag atggaccttc tctaagtact gttatgtata atcctaaaat tttgagccat
    13441 tctgttcaag atgcttgttt aggggaaggt gaaggctgtt tatctgttga tcgagaagta
    13501 ccaggatatg ttgttcgcca tgccaaaatc actgtgtctt attatgatat gaatggtgaa
    13561 aaacacaaga ttcgtttgaa gaattatgaa tcgatcgtcg ttcaacatga aatcgaccat
    13621 atcaacggtg tgatgttcta tgaccacatc aacgatcaaa atccatttgc actaaaagaa
    13681 ggcgtattgg tgatcgagta gagaattggc aggttccttc gatactatgc tacaagaaag
    13741 ctttataaaa aaggggagtg ctcaaaagct gcacttccct tttttatgtt tctccactaa
    13801 tcgaaattta gatagacatc tagaaatcgc aattccaaaa aattctgtgt attggatacg
    13861 catcttttct tattttttct tatttcttcc atatgggtgt ttatctcaga gatgatagaa
    13921 tagtttctag gataaaaagt agcagctacg atgttatcct tcgtagagga ttcctgcttc
    13981 agctagtttt tgtttgatgt ttttcagcat atgcttttct gggtagcgga tcgtatgcag
    14041 atggatgccg cctgttaatt cggataacaa agtagcatta cttttttgat aacgtctcat
    14101 aaaggaatcg atatcttgtg gcgttttgat gtgcaaagtt cctgctaact caccgtaaag
    14161 gggatgttcc acaattacat caatgatttc accaccattt tcaacgatta gtctcagttc
    14221 ttcttctgtt tgatcttttc catgctggac agcgatttta ctgatgtgcc cagtattggt
    14281 ttcttctgtt tcgaggatat aacctcttgc tgttgccacg atacttttac ctgcagctcg
    14341 tagtaaagca atatcgccta caatgatctg acggctgact ccaaaagttt tcgcaaactt
    14401 gctagcactc actggttttt ctgtatttgc caaggtttgc atgatttgtt ttcgccgtgt
    14461 ctctccttcg atcacactta ttcctccttc attttgtttt ggtattcgcg cgagtgtttc
    14521 atcaaatcaa catacgtgtc tgttattgtt tccttatatc ccttattttt cagcagacta
    14581 acaactttat ccaatacttg ctgctctctt tttgcatcat aaactgcttg gttttcttct
    14641 tttttcactt gtcctatttt agttaccgta tccattcttt tttctaataa agcaaccaac
    14701 tctcgatcga tcatatcgat cttatgacgt aatgtttcaa gagactcttc tgttagttct
    14761 tcctctactg tcttgatgat acgagcagga tttcctgcaa tcactacatt gtcgggaaaa
    14821 gatttggtca caaccgaacc tgcgccgact actacgttgt cccctagagt gacacccggc
    14881 gtgacgataa ctcctccgcc taaccaaaca ttattaccga ttttaatggg ttttgcgtac
    14941 tccagaccgc tgttacgttt cactggatgt agtggatgcg tcgccgtgta aagctggaca
    15001 ttcggagcga acatgcagtt atcaccgatt tcaatcgtgc tgacatctaa aaatgtacaa
    15061 ttgaaatttg cataaaaatt ctccccaaca aatatattat atccgtaatc aaaattgata
    15121 actggctcca tatagatttt tttccctgta cggccaaaag tctctttcaa caattgggat
    15181 cgcagttccg ctgattctgc ttggttgatg atttggcttt gacttcttgc aaacatacgg
    15241 tcggcaacca gctcaggatc attggcaaaa taaagctcgc ctgcaatcat tttttctttt
    15301 tcggttttga attcagtcat aatctgctcc taattcttgt atttttttga aaacaaccgc
    15361 tccgtcttct tgaataaacg tataaggctc cgctaaataa acaggcgttt gattgattac
    15421 taagattttg gctgacggct ggcgaaactg taccagatca caaaacggat gaacttgaaa
    15481 actcgttcca acaatgacga tcagctctgc ttggctgacg gcataagccg cctgctccat
    15541 gactccatct tgaaaccctt caccatataa cacgatgtct gggcgtattt gtcctccgca
    15601 tgcttgatgt ttgtcgctgt gaagatattc tttccagtct accgtttgtt gacattttcg
    15661 acaatgacat tgatacagac tcccatgaaa attgacgagt tgcgggcttt tagcctttgc
    15721 gtgcaaaccg tcgatgtttt gggaaacaac ccaaacattt ttttctttcg ctagatgtgc
    15781 tatttccaaa tgaatgatat tgggttttgc ttcaggatga taaatctttt tgacaaattg
    15841 atagaattta tccggttcat tcaccattgc ttgatgactc agcaagtatt ccggctgctc
    15901 catcccgtgg tagattcctg acaaagatcg atagtctggc actccagaag gtgtggatac
    15961 ccctgcaccg gtcaaaaatg taatttgctg ataagctaat atctcttttg ctgcttcgtc
    16021 tactgttaat tgtttcatgt tttgtcacct cactttccat tatagcccat actcgaacaa
    16081 atttattgtt gaaaagtcag aaaaatggga ataaaaaagt gttatgggca tttgagcaat
    16141 ttatcacaat gttcactgac aaaagaatga aaacgtgtta caatactgct gtataaaagt
    16201 gaggtgaatc gatatgttac cattctttat ttattggggc gttattctgg cttgtatcgc
    16261 ctggttagcg ttaagcgttt atttctctgt tttttatctt gtacgcaaag aaaatggaaa
    16321 tctttgggct tttgctttat tcaacgtgat tgccgcaatt gttttagcca ttacattagc
    16381 cgtatatcga acatggggct ggggaatcac ccaatactca agtttgattt atctaatatt
    16441 agccatttat ggcgtcgttg ttcttttgca agcgatccta ggacgtgagc caaagaaagc
    16501 ggcagcttaa atgatcgaga tggaactgga cacaggaaat tttctgttct agttcttttt
    16561 ttattgtatt acatacattt ttgtgtataa ttatactatc gtttgcaatg gatagatgat
    16621 tcacattttg attgtttttt cggtaaaagt aaagatttct atatttatca gtattttttc
    16681 aaaaaccagc agttatagat tgacaaagcc tctcgagtat cgtaaacttt ttttgtataa
    16741 ataactgaat acgagatgcc tcaatcaatg aggtagaggt cgcgagaatc attaaccctg
    16801 tggagtgaag caaacatgga gaagcaggaa tagggaggat cgccgaaatg tgcacttttt
    16861 tgcttggagt gtatgttggg acgcaagtta agagcttgcg gactgtctta gcagtgatgc
    16921 taagttgcgc tatgttttgt gagaggttta tcaccttatg gaattttagc ggccttttta
    16981 tatctataaa tcggtcgctt tttatttttt cagataaagc tttaccgcat ttcatctaga
    17041 ccgctcgtgt atcatataat cagaaaggga gaaaaaaatg aaaaaaaata tggtttcagt
    17101 tgccgtacta tttgcttttg tcttctcact attcagtgtg ggatctctgg caaatgcaga
    17161 tgaaaaaaca ccgagcgatg agtttcgcgt aggtatggag gctgggtatg cacccttcaa
    17221 ctggtctcaa caaacagatg agaataatgc cttgccaatt caaggccaaa atagttacgc
    17281 cggcggttat gatgttcaaa tcgctaaaaa agtagcagat ggtttaggga aaaaattagt
    17341 catcgtccaa acgaaatggg atggtctggc tcctgccctt caatcaggca agatcgacgc
    17401 gatcatcgcc ggaatgagcc ctactgctga acgacgaaaa gaaattgatt ttaccgatcc
    17461 ttattacgaa tcacaattag tagtagtagt tcaaaaagat agcaaatatg caaaagcaaa
    17521 aagtttagca gatctttctg gagcaaagat cactgctcag ttaaatacat tccactatac
    17581 tgtgatcgat cagataccag atgtgcagaa acaacaagca atggataatt tctcagcaat
    17641 gagaacagct ttagcttctg gaatgatcga tggttatgtc agcgaacgtc cggaaggtgt
    17701 aacggcaact agtgtgaaca aagacttgga aatgcttgaa ttcagcggtg cagatggctt
    17761 ccagacaaac ccggaagatg ttcagatttc tgttggtatg cgtaaaggcg acccggatat
    17821 ccaaaaagta aaccagattc tttctggcat ctctaacgat gaacgaacca aaattatgga
    17881 ccaagcagtc aaagatcagc ctgctgcaac tgatagtgaa gaaaaacctg gcatcatttc
    17941 tgatttcact acgatttgga aacaatacgg caatatgttc ttacgaggtg ctggacttac
    18001 cttgttcatc gcattgatcg ggacagttgt cggtactaca cttggattac tgattggtgt
    18061 cttccgttcg gtacctgaat caggaaatgc actgactcgc ttcttccaaa aaatcggaaa
    18121 tgcacttctt tctatctatg tcgaagtatt ccgcggaaca ccgatgatgg tacaagcaat
    18181 ggtcatcttc tacggtctgg cactcgcttt tggcatttca ttgaaccgaa cgattgccgc
    18241 tttgtttatt gtctctctca atactggagc ttacatgaca gagatcgttc gtggggggat
    18301 ttttgcagta gatgaagggc agttcgaagc tgcgcaagcc attggtatga cccatggcca
    18361 aacgatgaga aaagttgtta ttccacaagt tttacggaat atcttgccag caacaggaaa
    18421 tgagtttgtt atcaatatca aagatacagc agttttaagt gtaatcggcg tagctgattt
    18481 gttcttccaa ggaaactctg cttccggtgc gaatttccaa ttcttccaaa catttacgat
    18541 cgtcgggatc atctacttag tcatgacctt tacgatcacc agaatcctgc gggcggtaga
    18601 aaagaaaatc gatggtcctt cagcttatac caagcttgaa gaaatcgaca attcaaattt
    18661 gcaggaataa ggaggaagaa tcatgaatga aaaaattatt gaaatcaaac atctaagaaa
    18721 aagcttcggt gaaaatgaag tattgaaaga tatcagcgta acagtcaata aaggtgaagt
    18781 tgtgacgatc atcggttctt ccggttctgg taaatctact ttcttgcgct gtatcaattt
    18841 actggaaaaa ccaacaggcg gcgagatcat ttataatgga gaaaatgtat tagctcctaa
    18901 atataattta cctaaatatc gaacgaatct aggtatggtt ttccaatcct tcaacctgtt
    18961 caacaatatg aatgtcttgg aaaactgtat gtctggtcaa acaactgtcc tgaaacgaga
    19021 taaagaaact gccaaaaaag tagcaatcga aaatctggaa aaagttggta tggatcgtta
    19081 tatcgaagcc aaaccagcac aattatctgg tggacagaaa cagcgtgtcg caattgctcg
    19141 ggcattatca atgaatccag atgtattgct attcgacgaa cctacttcgg cgttagaccc
    19201 tgaaatggtt ggcgaagtgc ttaatacgat caaagactta gcacatactg gcttgacaat
    19261 gcttatcgtg acgcatgaga tggaatttgc aaaagatgta tctgaccgtg tcatttttat
    19321 ggataaaggt gtgattgcag aagaaggtac accagaagat atctttgtcc atccgaaaga
    19381 agaacgtaca aaagaattcc tttcacgtat tttaaatgct taggatcaat agctgattgg
    19441 gacaaagagc tgcggtgttt accgcagttc ttttttatga attactcata aaaggtaaat
    19501 ttctgaa
//
LOCUS       DAYYGO010000053        18568 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000053 DAYYGO010000000
VERSION     DAYYGO010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.054"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            118..819
                     /locus_tag="ACHA5T_001536"
     CDS             118..819
                     /locus_tag="ACHA5T_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM8887114.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1428..2444)
                     /gene="tsaD"
                     /locus_tag="ACHA5T_001537"
     CDS             complement(1428..2444)
                     /gene="tsaD"
                     /locus_tag="ACHA5T_001537"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFM8887115.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2441..2905)
                     /gene="rimI"
                     /locus_tag="ACHA5T_001538"
     CDS             complement(2441..2905)
                     /gene="rimI"
                     /locus_tag="ACHA5T_001538"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFM8887116.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2912..3454)
                     /gene="rimI"
                     /locus_tag="ACHA5T_001539"
     CDS             complement(2912..3454)
                     /gene="rimI"
                     /locus_tag="ACHA5T_001539"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFM8887117.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3438..4160)
                     /gene="tsaB"
                     /locus_tag="ACHA5T_001540"
     CDS             complement(3438..4160)
                     /gene="tsaB"
                     /locus_tag="ACHA5T_001540"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFM8887118.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4351..5331)
                     /locus_tag="ACHA5T_001541"
     CDS             complement(4351..5331)
                     /locus_tag="ACHA5T_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8887119.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5355..6839)
                     /gene="galT"
                     /locus_tag="ACHA5T_001542"
     CDS             complement(5355..6839)
                     /gene="galT"
                     /locus_tag="ACHA5T_001542"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFM8887120.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6851..7840)
                     /gene="galE"
                     /locus_tag="ACHA5T_001543"
     CDS             complement(6851..7840)
                     /gene="galE"
                     /locus_tag="ACHA5T_001543"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFM8887121.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            8088..8255
                     /locus_tag="ACHA5T_001544"
     CDS             8088..8255
                     /locus_tag="ACHA5T_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFM8887122.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(8317..10128)
                     /locus_tag="ACHA5T_001545"
     CDS             complement(8317..10128)
                     /locus_tag="ACHA5T_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFM8887123.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(10125..10490)
                     /locus_tag="ACHA5T_001546"
     CDS             complement(10125..10490)
                     /locus_tag="ACHA5T_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFM8887124.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(10653..11048)
                     /locus_tag="ACHA5T_001547"
     CDS             complement(10653..11048)
                     /locus_tag="ACHA5T_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM8887125.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(11066..12028)
                     /locus_tag="ACHA5T_001548"
     CDS             complement(11066..12028)
                     /locus_tag="ACHA5T_001548"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFM8887126.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(12028..12240)
                     /locus_tag="ACHA5T_001549"
     CDS             complement(12028..12240)
                     /locus_tag="ACHA5T_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFM8887127.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(12261..12959)
                     /locus_tag="ACHA5T_001550"
     CDS             complement(12261..12959)
                     /locus_tag="ACHA5T_001550"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFM8887128.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(12979..13521)
                     /locus_tag="ACHA5T_001551"
     CDS             complement(12979..13521)
                     /locus_tag="ACHA5T_001551"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFM8887129.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(13653..14657)
                     /locus_tag="ACHA5T_001552"
     CDS             complement(13653..14657)
                     /locus_tag="ACHA5T_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFM8887130.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(14654..15643)
                     /locus_tag="ACHA5T_001553"
     CDS             complement(14654..15643)
                     /locus_tag="ACHA5T_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFM8887131.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(15640..16446)
                     /locus_tag="ACHA5T_001554"
     CDS             complement(15640..16446)
                     /locus_tag="ACHA5T_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8887132.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            16612..17568
                     /locus_tag="ACHA5T_001555"
     CDS             16612..17568
                     /locus_tag="ACHA5T_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8887133.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(17645..18163)
                     /locus_tag="ACHA5T_001556"
     CDS             complement(17645..18163)
                     /locus_tag="ACHA5T_001556"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFM8887134.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(18251..18400)
                     /gene="rpmG"
                     /locus_tag="ACHA5T_001557"
     CDS             complement(18251..18400)
                     /gene="rpmG"
                     /locus_tag="ACHA5T_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM8887135.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
BASE COUNT         5546 a         4010 c         3211 g         5801 t
ORIGIN      
        1 acaaattata tttgtaaagt attaaaactt gtttctaatg ttggtatgtt aaaactttac
       61 aaattcttta aaaaaagtga caataaatat agtgaacaat aaggagagga ctttttaatg
      121 cgtgttaata aaatcgattt tcacttttca aaactcagat atcaacctga ttttgaacat
      181 ttctcggatg aagagttcca acagatcaag gaacatactg ttcttcgttc ttataaaaaa
      241 ggacagatcc ttttcgacga tggtgatccc agacaacggt tgtatatctt gaccaaagga
      301 ctcgttcgtt tagaacgtta tgatgaaagt gcaacttatt actactatga ctatgtgaat
      361 acaaataacc tttttcctat gtcaggaatg tttggagaag agacctacac ttatacagct
      421 caagcgatga ctgatattga aacattctac cttcctgttt cctactatga aaaattagca
      481 cgaaaaaatc ccgaacagtt gatctatatg atacaaaaaa tttctaaagt agttgaaata
      541 catgaattgc gtatccaaat cggattgacc tctagcgcct ttgaccgggt aaaacaaaat
      601 ttattcattt tgaaagaaga attgggcgta ccaacaaaag atggtcatat caccattcct
      661 tatccgatca ccttaaaaga attagcagtg aatagcggca cgacacgtga aactgctgga
      721 caagtaatca aacaactacg tgattccggg ctgttagatt atgacaaaaa gcgttttact
      781 ttctgtaaga cagctactac tccagctatg agagaataac gcgaacaaaa ataagcagtg
      841 accctgataa atatcagggt cactgcttat tttcctccct aaaacttctg aaagatccgg
      901 cagttttaga tcactggaca agactcaaag attttctatg tgtgtttcag ttatgtaata
      961 ttcactgttt gcaaaaaata ctgttagatt aactagatga agcgtcttgt cctcatccga
     1021 tctagattat tcgatattcc tcgacaataa acgattactc agtgagggca atcagaggaa
     1081 cggataatga taaataagct gtaaaaaata gcaaaataac aataatgatt aaaagtatgt
     1141 aaaaaatagg gctttgacaa aaacgtgtca cagccctatt tcgattaaac ggtgtgtacc
     1201 tgtcaactct tcggtcacaa gtcataatgt tcataaacat gaggagctgt tttttgaaat
     1261 tgttccttgt gaatcagagt taaacgacag gcttacgaac tcttgattaa acggcgttca
     1321 aaagcttgca tacgcggtaa taagaacagc caggtcaaaa acatgagaag ccgtttttcc
     1381 tgggctgttc ttattacttg atgcagggca gctttttcac aacctcttta ttcagataaa
     1441 accaagcttg gatcagcatt caactgatag ctagcaaaat ttcttttttg catttccaca
     1501 tgtgctgctg ctccaatcat tgcagcatta tctccacaaa gtcgaagagg gggaatcact
     1561 agttctacct cagggagttt agctgccaaa gcatcctgca atccttcacg caacccttga
     1621 ttggccgcta cacctccagc tataatcagt tgcttgactg gataattttg acaagctcga
     1681 agtgttttag taatcaatac atcgatcaca cttgcttgaa agctagctgc taaatcattt
     1741 ttgtctagat cttctccacg ctgttgtgca ttatgcacca aattgataaa tgcacttttt
     1801 aatccgctaa aactgaaatc ataattatct tcatggatca tcgctcgcgg aaaatggtaa
     1861 ttatcttttc cctgatgagc tagctgatcg atttcttttc cgcttgggta agaaagacct
     1921 aatactcttc caactttgtc ataagcttct cctgctgcgt catctcttgt ctccccaatg
     1981 atttcatatg atccatcttc ttgcatatag actaattctg tatgtcctcc gctaacaaga
     2041 agtgccatca atggaaactg gaatggttta accagacggg ctgcatagat atgccctgcc
     2101 atatggttta caggaataag cggcaattgg tgtgcccaag caaaagcctt agctgcagaa
     2161 ataccaatca gcaacgagcc gacaagtcct ggtccataag tgacagcgac tgccgataaa
     2221 tcttctgccg acacaccagc ttctaccagt gcatcttcca agcataaggt gatttgttct
     2281 acgtgatggc ggctagctac ttcaggtact acgccaccaa aacgtttatg actgtttatt
     2341 tgagaagcaa cgatgttaga taagatctct gttccatttc taacgaccgc tacactcgtt
     2401 tcatcacaac ttgattcaat cgccagaatc aattcttcat tcatttcatc gtctccgttt
     2461 ttaactttct gctcataatg atcgcatctt ccgtcggatt gcgataatat gatttccgtt
     2521 tacctaatat acgaaacttt tctgattcat aaagttttct tgctgcttca ttagatagac
     2581 gtacttctaa aaaaacatgc agcgaatctt ttatttgttc cgtaatcagt aattgtaaca
     2641 aatgccgtgc atgccctttt ccttgttctg aaactgcaac agcaatattt gtgatctcga
     2701 cttcatctat tactttcgag tatccaataa agccacagat ctggttatct tcagctaaaa
     2761 gcagataatc agagtgaggc tgttggatgt ctgccaaaaa ctgcattttc gtccaaggtg
     2821 aaccatgtgg ataagcattt tcgctaatca tccatacgca ttctgccaag tacgcttcat
     2881 caaaatcttt ttttctgcaa atcaactaat ctcactcatt ttctaaaagt tttatcatat
     2941 ccaacgcatc ttcttttgtt tcagtgtaat atcttttttt gatcgttgtt gcttgaaacc
     3001 ccaatttccg ataaagtctt tgtgcatctt gattactcat acgcacttct aatgacatcg
     3061 tctcacagcg attcattata gcaaagttct cgatttcgtc aataagcagc gaaccgattc
     3121 gtttacgctg atatgccggt cgaacggcaa tattcgtgat atggcaatct aaaccgaacg
     3181 ctcttgaacc gataaaacca atcattcttc cattatcaaa gatacctaga tatagatgag
     3241 gaagcggtga cttcaattct gatataaagg cactttttgt ccatggcaat tcgccattat
     3301 agacttctcg ttcgacatcc agcagctctt tgatatcttc tgtagtcaat tctcttagta
     3361 cgtaacgacg atcgatttgt tcgactgttt tttctgtata aatcatttta tcttgaaaaa
     3421 agcttctggc atacgctcta aattttttca acataacttt cgtctccagg tgtgtgcgtc
     3481 tccaaccatt tttcttctgc ttctactcgt tttaaatagc gaggaagaaa agcttgtttc
     3541 tctgcaactg gttcaactaa agcacctaat tgagccacag ttacaccact tggaatatcc
     3601 catgctggaa ctgtattaat catcgcacta ggcaatattt cctttatttc agagaagaat
     3661 ttgtgacaat cactcccgac gaaataaata gcttcattca gcttttgtag ttgtgtgaac
     3721 aaatcagaag cagaaatatg tacatcttca agtacagttt ctaatctatt ttctttccag
     3781 cggtatgccc cagcgtatac attttttctt cgagcatcaa acatgggcac aataatatta
     3841 tccacaccga tgcaattagc cgcgatggtt ttcagactag atactcctac tagctcttta
     3901 tctaatgtat aagcaatcgt ttttgctgtc gttacaccta tacgaagccc tgtataagaa
     3961 ccgggaccat cagaaaccgc gattcggtca atgtcttttg gacttatttg aagatctgca
     4021 aacaattggt cgattgcagg catcagtgtt acactgtggt tgcgtttgat atttgtttgt
     4081 atttgtccaa gaatttgttc gtcttctacc acaccgatcg ccaaagtttg gtttgcagta
     4141 tctattccta atgtaatcat atatttgatc ctttctgcta tgacattctc gccttccatt
     4201 gtagcatact tttttattct gaggatgaaa ataccaaagg caaaactagt acaataatac
     4261 atgattatct gctggcgggc aaattttgcc ccttcctgaa acacaaaaaa gctgcggtat
     4321 cactccattt tcccacagct tttccatctt ttatttcgtt gattctcgaa taatcaactc
     4381 tgtttccaca ataatctttc ttgaaaccgg tgctttggaa ttacttaatt gaagaatcgt
     4441 ctccactgct agctctccca tccattcggt ataaactttt acagtagaaa gcgagggcgt
     4501 tacatatttc gctacactga catcattgaa gcccatcaca gaaatatctt ctggtaccac
     4561 taaacctttt tcttgaatcg ctttcagtgc gccaatcgct aatgcatcat tagctgcaaa
     4621 aagcgcatca ggaacatgcg gattcgtttt taaatattgc ttcactgctt gatatcctga
     4681 ctcgacagaa aaatctgctt cgataattgg accttcgtcg tagccttttt ctgccaaaac
     4741 ttgttgaaat gccataattc tgggatcttt tagccgctga tggtttttct tcgtatattc
     4801 tgttccagca agcatagcga tatgttgcgc tttatgggag acaaagtgtt caatgacttt
     4861 ttgaacactt gacttaaagt cgacaactag agagtcgatt ccttgagaag tgccgtcaga
     4921 atctaccaaa agcaaggcat tttgggatcc tttcatttct aataactgag cttcgtcgaa
     4981 ttttcctagt gcgatcgttc catctgccgg aagatcacta atggaatccc acgtttcttt
     5041 caataaagta acgtttgctt cttctgcctt tttttcgatt cctaggcgaa tagataaata
     5101 ataaagatct tcaagctctt ccgtatcgtc gtaccactgg atcaggcgaa gcgtttgctt
     5161 agtatttgct gcatttttac gatgtttcgt ataatttaag cgttctgctg cttcaaaaat
     5221 tttttgcttt gtggctaagc caacggaaag atcaggatca tagttgagaa ctcttgaaac
     5281 agtagcagga gaaactcctg ccaactgcgc aatgtcttta attgttgcca tttgtctctc
     5341 ctcctttttt acgtttataa aatcgcagta aatcgttcaa acgctgcctg tcctttttca
     5401 tctcgcttga atactccagc gtcttccagt acacgtgcaa aaacctgacc tacagatttt
     5461 tgtacgacat cggtgaccgt atctttcgtc aaatcaggat gttctttttt taattgttct
     5521 gcccacggac gatgatattc agccatctgg ttttcttctc ctaataagaa atgctctact
     5581 tctactagtt ctggtttcaa acgaggtggt aaaacagcaa gccccattac ttcgatcaac
     5641 ccaatgttct cctttttgat atgttggaca tctttatgtg gatgaaaaat cccatcagga
     5701 tgttcttctg aaacattgtt gtctcgaagg acaaggtcca actcaaagta attgtccctt
     5761 cttcgtgcaa ttggcgtaat cgtatggtgt ggtgtaccat ctgctgaaaa agctctgata
     5821 gatactgttt catccgaata tgctttccat ttttccaata ctgagttagc tgcttcaata
     5881 agcaagtttt tctctgttga ttgcaaacgg atcacagaca tcggccattt cacgactcct
     5941 gcgatgactt gcggataatc ttctagtttg ataagttttt ctatcggtgc ttttgccata
     6001 ggaaattcat gacgtcctgc ctgataatga tcatgagaaa gaatcgatcc tccaacaatt
     6061 ggtaagtcag cattcgaacc aacaaagtaa tgaggcaaga cttcagtaat ccgaagcagt
     6121 ctagcaaatg tttcctttgt gattttcatc gggcggtgtt gttctgataa aatgatcgaa
     6181 tgttcattgt aataagcata aggggaatat tggaatcccc agctttcacc gtctagattc
     6241 attcgaataa tccggtgatt ggtgcgggct ggatagttca agcgacctct atatccttcg
     6301 ttttccatgc aaagcatgca ggtaggatag tctactttta ccgcctcgcg ttctgctgcg
     6361 atttgttttg gatctttttc tggcttagat aaattgattg tgatttccag ttcaccatac
     6421 tccgactctg ccggaaattt gatgtttttt gcgatggctc ttgttttgat gtagtcattt
     6481 ttcttactta aattaaagaa atactcagtt gcttcctctg ggcttttcga atagtgctgt
     6541 gcaaagaatg cattgacgac tgaaggtggc ggtgtgagaa aatccattaa ttgcgcttca
     6601 aaaatctctt gttctgctgg caattctgaa agaacaccat tttctcttgc cttagcgaca
     6661 agctgatcca ataagtctac agattcttcc ttaactggtc ttacttcaat gctttctaga
     6721 gcatcttctc ctaccatccc taaaagacga ttctgtaaat ataagcggtc catttccatc
     6781 catccgccag cttcgatcgc aagtgtggca aaatcacaaa tcgtttgact gattgacata
     6841 tctcattccc ctattcttca taaccatttg gatggctgac atgccaatcc catgctgttt
     6901 tgataatcgc tttgatatct gtgtattcag gtttccagcc cagaatctct ctcgcttttt
     6961 cacttgaagc gactaatcgg ctaggatctc cagctcttct tggtgctatt tctgctggaa
     7021 tttcttttcc tgtcacttct ctagctgctt ctagcatttc cttgactgag tatcctttgc
     7081 tgctgcctaa attgaagaag ttgctttcat ttccttcttt caagtattcc aaagctaaaa
     7141 tatgtgcagc aatcaaatct tctacttgaa cataatctct gatacatgtt ccatctggtg
     7201 tatcataatc gtctccataa acagccaagg cttttctttg accaagagct acttgtaaaa
     7261 tgattgggac aagatgcgtt tctggtgtat gatcttctcc gatagatgca tctgcttttg
     7321 ctccggctac attgaagtag cgcaaagcga catagcgcat gccatatgct tggtcacacc
     7381 acttcatcat tttttccatc atcaatttac tttcaccata cggattttta ggattagctg
     7441 gtgtatcttc tgtgattgga gactctttcg gctctccata agttgctgca gtagaagaga
     7501 atacgatttt attcacgttg aactcgtgca tgacttcaag taagatttgc ataccgtata
     7561 cgttgttgtt gaaatacatc aatggttttt cgacagattc tcctactaaa gagcttgcag
     7621 caaaatggat tactccttca atcggttcct tttcaaatac ggaacgtaga aattctttat
     7681 cccggatgtc tccttcataa aaatgagcat ctggatgtac tgcttgtttg tgacctgtga
     7741 gcaaattatc taccacagct acttgatacc ccttttgaac tagctggtct acagcgtgcg
     7801 aaccgatata accggctccg cctagtacta aaattgtcat aaaaaacact cctttgatta
     7861 gtaaaattgt gtttgttgat tctatataat ttaaaaaacg aagtttactt ttatggtgaa
     7921 aacaaacaaa gtaaaatttc gtaaattcat atggtaaatt tataataaaa catttagtaa
     7981 acattttcaa gggttttttg caaaggttta caaacaaacg taaaactagc ctttttttct
     8041 cttttgttcc ataatagata taacaaataa taaatgaggt ggattcaatg aaaaaatttg
     8101 tatctggaat attagtagga agtctggcaa cagccgctgc cgttgctggt ttggttgctt
     8161 ctgtgaaaaa aacagtgatc gatccaattg acgaaaaaga agcaatgatc gaagaaaacc
     8221 gtaaaaaagc aatgcgtaaa cgcgtttctc gttaataatt atttaaaaat acgcatgaag
     8281 actttctaaa aaacagataa gtcatcatgc gtatttttat ttgtcagtca atataagttg
     8341 tgctatttta tctgcctgtc tcagattcca tggatctgta taaggtctag ctactgctgc
     8401 agttagccaa tccacacctt caacaggtat tccatagcaa taaaagtcag actggatttc
     8461 ccctgaagca tcaatcacgc gattcgtacc tcgctcaaca tctatagctt ctgaatgata
     8521 acttcctgta ttatctgaaa atgaaaaagg gcgaacaagt ttgttttcct tcaaattttt
     8581 tatgacagga ttacttgaac gagaaaagct tgtttgtggc aatcgagctt caatcaaata
     8641 gcgactgctg atttctttat cttgtagaga aataacatag cggttgtgct tttgctgaat
     8701 atcaaatggc ggttcgatca aatgaaaaat acccgcatct atcagtgctg ctaattcttt
     8761 cgtccgaatg attggcggac cgatcgttaa aaagctattt agatgagtaa accatcccca
     8821 tagtatttct ttgtattcct tagcagaaaa aacgtgccat tcaatagcct gccggattgg
     8881 atcacgccag tcttttaatg catcaaatgt tgaagcaaat gctcctgtgc aattgccttt
     8941 ttccgcttct tttatctgcc cttcaataaa tattcggcta gcttctgaaa agggctcatt
     9001 ctgataggca agaggagttt ctaaatacga ccaatcccat ctataaggga ttagtgctgg
     9061 atgctttccg agacactcct ctgaagagag ggagagaaaa tcatgctcaa aatttttctt
     9121 tggaatcggc aagtgatatt cttcgatgat tttcttgtaa taaaacaact caacgtcttt
     9181 tttcagatag tcaaaaaatg tttcgccagt caaaaatccc gactgatgcc attgctccag
     9241 gttttctttt gttagtaacc gtggccatgc cattgctccg ccttgctttt gatttcttcc
     9301 tcgagggtaa taagggagcc cccttcttga tccactgtat acaatcggtt ccttgcctga
     9361 tggatgatag accagttttc ccttttcgtt ctcgaagcgc cctcctcttg atactgtaaa
     9421 caatccgaca taatcaaaaa aacttaagcc tagacctctt aaaaagacat tttctttagg
     9481 cggtatcttc gaaagatcta catctgcagg attagaagga agctgataca gtaaaacatt
     9541 tttctttgcg tagtctaccc acactctttc ttcttctgtc tcatcatttt cccagtgacc
     9601 gcttgctaaa acaagctgat cgatagaata gctttgctga tctgtctgta aaagataacc
     9661 atcttttctt ttgcttactt tttttactaa agaataaaca aactgaatag aagctggaaa
     9721 ttcttcttgc aactttgaga aaaaccagcg ttgatagagt ccgtataaac atcgagtaga
     9781 ttgctgatct ttttccagac gattgatttc ttctataatt agttgcttat ttttggttac
     9841 cgtctgttcg atataatctg tagcaggtcc tttactccat tgaaacaagt tgggtccctg
     9901 tgttaccaat cctccactgg acaatgtttc atctgtaaaa agcgtcactt gttgagaaac
     9961 agaattcatt aataactccg gtggctgatt caaccgccaa actcgacctc caggaccatt
    10021 gggatcgaaa agaaaaagtt caagagaatg tctgtcctga ttattatgga gaatcctttc
    10081 tgctacactt aatcctctag gtccggcacc aatgatccct attttcattt gcatgcctcc
    10141 atttcgataa attttttcaa taaaacagca tggttctgat gttcattttt tgcaccataa
    10201 acaaaagtaa tgactggatg ttctttcata agttgactga gtttaaaaat agcttgcttc
    10261 ttttcttctt cgaaaatcaa ttcttgctgg tatttctttg caaatgaagc aaatcgttct
    10321 ggtacatggt caaaagtttt gcgtgcttca ttactaggtg caatttcttt caaccacaga
    10381 tctaactgcg ctttttcttt tgaaattcct cgtggccaga atctatccac gagtattcta
    10441 tagccatcct ctttttcagc atcaaggtaa acacgtttta atttgatcat agttgtttcc
    10501 ttctttctac cttttcgctt agcttatata tgtagataat agcacaaaat aaaaaaaatg
    10561 aacggcacaa ctatccattg cgccgttcat gtgatcttgg tcaaaatcta atgattgatt
    10621 cggtttttaa agctgtcata caatcatctg ttttatactt ttttactttt gatttttcca
    10681 gaccatccgt catatccacc cttaagtaaa taaatctctt gataacctgc tttacgtaat
    10741 ttatttgctg tacggatgct caaacttttc ttttgatcgt aaatatagac aggctggtct
    10801 tttcgaatag atcctaatga atctttcaaa acagtgtaag ggatgtttct tgcacctaaa
    10861 atatgcccag catcaaacac atctttttcg cgaacatcga ttacttgtgc ttttctcatt
    10921 ccttctcgaa attcttcttc tgtcaaagtc gttgctgaac gtttggccat tactcgtaga
    10981 tagacttccc aaaggatgat tgcaagcaca atcaatagca aaatgatatt aattacccat
    11041 aaaaccatgt aatgcctccg tttttttaag caaatttcaa ggctaatgag gcagcaccaa
    11101 tgacaccagc ttcattacca agctctgcta atttgatttt tgttgattct cttacttgcg
    11161 ggaaagtaaa ttctttaaaa tatttctcta cacggctacg taaaaattct cctgctgccg
    11221 atactcctcc acctaacacg atacttgacg gatttaatgt attccccaag tttccgcaag
    11281 ctaaacctaa gtagaagcat acgcgatcaa caaccatcaa tgcaaacgga tcgtcttctt
    11341 gagccatttc aaagatatct ttacttgtta cttcttctcc gttgtctagc attgctttca
    11401 gttttgaatc ccctgcgtat tcttctgcca agaaacgacc taggcgtacc acacctgtag
    11461 cgctagatac agtttctaaa catccgcgtt taccgcaagt acattcgaac ccttcaggat
    11521 ccacagtgat atgtccgatt tctcctccag ctccgctcac gccgtgaatc aaatttccgc
    11581 cagcaacgat acctccgcca acacctgttc ctagagtaac gaatacaacg tctggatcgt
    11641 tttctccagc acctttccat cgttcaccta aagcagcaac attcgcgtca ttatcgatag
    11701 cgaatttgat tcctgttcct gcctcgatca gttttttcac aaattgaact tctgtccagt
    11761 ttaagttata cgcaccaata accgttccag cttcgctgtc cacgcttcct ggggtaccca
    11821 tgccgattcc gataaagtct tcggctttca tattatataa attcaaacga tgattgattg
    11881 attcaatgat ttcaggaacg atatgcttac catcatccaa aatattggta tcgatgctcc
    11941 atttttgctg aatctctcct tctggtgtta aaattgcaaa ttttgctgtt gttccaccta
    12001 aatcaatccc aataatcttc ttgtccatta caaatttcct ttctttcggc tttcttcaat
    12061 tcggtgctct ctttttaata tgcgccacgc tgtcatgtat gtatcatgat caatcaaacg
    12121 gctatcatac agattacgca attcgatagt cattaattca atgtcataga tgcgtgcacc
    12181 cacatagata aagatgccaa attttttgaa cagctgctgt acatcatata agctctccat
    12241 ctttaagcac ttccttcata ttatacaagt aaaccatatt tttttaaacc cagtaaaata
    12301 catattacaa aaagaaatac aaaaactagt gtagaaagaa tcctttggtg tatcgaatag
    12361 cttgtctgtt gtttcggtag acccaaaaca ttccctaaca ctaatccgcc aattagccca
    12421 ccgacatgtc cccaaacatc tacagaggta tccagaagac caaaaacaaa tgtcaacaca
    12481 ataaataaaa gaaaatgtct gactagctgc tggatcgctg ggttatgttt aaaatgaaac
    12541 cctaagataa acaacgcacc gaacaaaccg aatatcgaag tacttgcacc accagacaga
    12601 acattcgctt cgttaaaagc aaagcttgct gtatttccca ttatcccgct gaacatataa
    12661 atcaagaaaa aacgccaatg gccatagatt gcctcgattt gctgccccat aaaatacagt
    12721 atcagtgagt tcattacgaa atgcatcagt ccaaaatgga tgaagattgg tgtcactaaa
    12781 cgccaccatt cattgaagtg aacaagataa ggaccataca ttccggcata ataagggatc
    12841 tcaagtcgcg gaaataattc catcatgagg aagaccgctg tctgtatccc taaaaaagca
    12901 taggtccata acggtcgtct taaccatgca cgcaatttta tttgctgttg ataattcatg
    12961 aaaacctccc atttcatttt agtcaataaa caattgttgg atcttctgat caaagctctc
    13021 cggttgccat tgttcattca attgttcacg aaaaacaagg ctacatgttt ttttatgaaa
    13081 attatttaaa taccggtcgt aataaccacc gccaaaaccg attcgataac cggctctatt
    13141 gtagataata cctggtacaa tgaggagatc gatcatctcc ggcaaataga gcttctctga
    13201 ttgtggctca agaacaccaa aactcgaacg tacaagaacg gactgttcat ccatttcata
    13261 aaacgccatc tgacgtttcg gcaaagtttt aggtacaact attttctttt gttctttttc
    13321 tgctcgttgg ataaccggct gtgtatccag ttcaaattca ttcgataaag tcgtccctat
    13381 cacttttgct gacttccagt actcgctggc gaatagctgt ttatagattc gctgttcttt
    13441 ttcctttttc ttgccacttt tggccagatt tttcagcttt tcgattcctt tccggcgtaa
    13501 tcttactttc aattggtcca aaaactcacc cttccttctc tcataaaatg gtataggaaa
    13561 aaaagaaaaa tagcaatcga actttcaaat aaatgcataa atagaataaa aagaagacct
    13621 agaaatgatt ggccttcctt ttttaatgaa tttcaactgt tcagcgcgtg tttttgtctg
    13681 ataaaaagtg catacagaaa atatggcgca ccaaaaaggg caatcaaaat tcctgtaggt
    13741 acttccccac ttggtagaat cactctagca accatatctg cactaagcag aaaaatggaa
    13801 ccgaacaaac cgctcagaac aaaggtccag ttattttctt tacgtacgac tagcttcgca
    13861 gcatgaggag ctagtagtcc tacaaaagat aaactacctg tcaccgctac actaccgctt
    13921 gccaaagcaa cagctaacaa caaaaggatt ttttgacttt tagccacgtt aaggcctaat
    13981 ccttgcgcgc gttcttgacc aaatgatagc aatacaatcc tctgtgcata aagtcctcct
    14041 agcggaatta agataaccgc ccatggaagc aatgcccaaa cataaggcca gctcgcaccc
    14101 cagattgatc ccgctaacca tgcattcaca aatccataat tttccttaga taattggatc
    14161 gtgcatagca atgttaatgc agaaaggccg gcattaacgg caataccgga caataagatt
    14221 cgattcatac ccaaaaagcc atttcgatca taggccgcaa gatagacaca gcaagcagct
    14281 aaaacaccac ctagaaaggc tacaactagc aatagaaacg gcagcgaata gtgcgaaaag
    14341 aaacctaaat aaagcatcac taaaaatcct gctccggcat taatgcccaa tatactagaa
    14401 tctgccaatt catttcttgt tactccttgc aagatataac cagaaacgcc aagacaagct
    14461 cctgctaata aactgatcag taatcgagga actcgaaatt ctctaacaat cagcgccaaa
    14521 gaagatgatt ctttccctat gaacgtggca aaaagctgat ccaggtaaat aaaaggcgta
    14581 ccaatcatta aactacctat agacaggatg acaagtacaa aaagcaagaa atagaacgtc
    14641 caatatattt ttttcaccgt tgttccctcc ttacttgtat cagtaaaaac ggaacgccaa
    14701 tcaatgcaat gactaatcca aaaggtgttt caaaaggggg attgatcatc ctcgctgtaa
    14761 aatccgccaa cataaccaat ccaccgccaa ctaaaaaaga gacaggtaga acataacgat
    14821 agtccgttcc aaccattccg cgaacaactg gcggaaccaa cagcccaata aatgaaacag
    14881 gtccgactaa cgaaacggca atggctgcta acactgcaac tagacagaaa acaatcaacc
    14941 gcgttcgttt aggatgactt ccaagagcta atgcatgtgt ttcactcaaa tgaatcaaag
    15001 atattgcttt tcctaatgaa aaagaaacca aaacagcaag tatatataaa ggaaacaata
    15061 ctgctaattg tttccatgtg atattggcag ctccgcctac aaaccaaaaa gcaagatcct
    15121 gctgcagatt aaagaattgg gtaagtgctt ggctgatagc agtaaaaaaa gaactaatag
    15181 ctacgccagc taaaatcagc tgtaaaggat tcatgcccat tttcgaaaac tgcaaaaaac
    15241 caaatacgag caaagttaca gcaaaagaac ctgccaatga cattagaaac acagaaaaat
    15301 cagaagcttt aggccaaaaa gcaaaactaa gggccattgc caacgaagca cccgcgttga
    15361 ttcctagtaa gcctgaatct gctaaaggat ttcttgtcac tccttgcatc aatgcaccgc
    15421 tcaatgcaaa acaactccct acaagaaatg ctcccgccac acgaggcaaa cgaatatttc
    15481 gtatgatttg gtgactttgt tttgatctat caaaatcaaa caacgcctgc caaaaatctg
    15541 ctttttccac agtcgctgcg ccatttgaaa gagcaacaaa aaaaataatc aaaagaacaa
    15601 aacagaccaa aagcaggagt ctatttttct tcatagtcct cataattgat cctcctgctc
    15661 ttttggcaac aaatcatagg ttagaaaaac tggtttattt gttcttggaa gcgtgacaat
    15721 cgttgcatca atctcaaata tttttttcat acactctttt gtgatcactt cttcaggtgt
    15781 accttctgtg atcaacttac cgtttttcat tgctaataaa taatctgaaa aacgtgaagc
    15841 atgattaatg tcatgaatcg acatcaagat cgttgtttgt ttcatttgat tgatttcagt
    15901 caataactgt aggatctcta attggtgtgc cggatctaaa aatgttgtcg gttcatccaa
    15961 gatcaagata tctgtttcct gagcaagcgc catagcgatc cagacacgct gggtttgtcc
    16021 accagacagc gttgcgatat cccgcatacg aaatccagct agacctgtag catccaacgc
    16081 ccactcaatt gcttcatgat cttgcgtgtc catgccactg aacagttttt gataagggta
    16141 tcgtccgtaa gatactactt cttctactgt catacctgta gggtgttcac tagtttgtgc
    16201 taataatgca attttttttg caagttcttt tgaactgtat gtagagatat cagagttatt
    16261 gacaatgatc tttcctgatt tcacaggtag tatacgggta aaacttttca ataaagtaga
    16321 ttttccactt ccatttggcc caatcaaact agtgattttc ccttcaggaa tcgtaacaga
    16381 catgttttca atgatttttt tgttttcgta gccggctgca atgtcagctg ctcttaatct
    16441 cgtcatttca cccatccttt cgctgaaaat cattctcaat tatgacgctg tttcaacatt
    16501 tcgtcaagcg ctactttcat atctatagcc aaatgaagcg gttgacagcg ttcgaaatat
    16561 caggaataat aaccaatgag aaagattctc aattatgtta ggaggaaaga aatgaaatta
    16621 aaaacaaaag tattcacatt atctagcata ttgctgctgg ctggagtact gagcgcttgc
    16681 tccccctctt cagataaaga aacagctaca actgccaatt cgacgacttc atccaatagt
    16741 actcatacag tcacagatac acttggacat aaagtagaga ttccaaacgc gcccaaacgg
    16801 atcatcggca gttacttaga agactatctg attgcactag gggaaaaacc tatcgcccag
    16861 tggactgttg gcagtggttc cattcaagat tatctgcaag atgatttaaa ggatgtccca
    16921 acgatttctt atgaccttcc ttatgagaaa gtgttaagct ttgagcctga tttgctgttg
    16981 atttcttctt ccgctactgt cgaaggcggg aaatacgaac aatatagcaa aatcgcgcca
    17041 acttatgttg taaaaaacgg aacagatgtc acatgggaag aacaactaaa agatgtcggc
    17101 aaagctttgg caaaagaaaa agaggcagaa aaaatcattt ccgattatca aaaatccatc
    17161 aagaaaactc gagaagaact agcagataaa atcgagaaca agacagctgc agtgttgtgg
    17221 gtgaccaaca actcagcctt tatggtcagc aaaaatcggt caagtggaag aatcgtatac
    17281 gacgatctgg aatttggtgt tcctgatcta gtggaagagg tctccaaaaa agccacttct
    17341 gactggtctg ctgtttcctc tgaaaagcta gctgaattag atgctgacta catcatcctt
    17401 gtgaacagtg acaaagatgc tgccatgttc aatgaaccaa gctggaagaa tctaaaagca
    17461 gtgacagaca atcatgtact gcaattcgga cctgaatcta gttggctata taacggacct
    17521 attgccagtc aggaaatggt caatgatatc aaagaaaaac taaattgatt ttaactaaaa
    17581 aaagcatctt cacgattagt cgtgagatgc tttttttctt attaacaaac tagacgcata
    17641 gattttattc ttcttttgaa gcaaccctat tttttactct ttcccatttt tgaaaacgat
    17701 gggcatatag attttcttga tccgttatcc cctcatactc ctctataagg gcgaagtctg
    17761 aaaaatcaat attcccaata tacgtatcgc cttcaaacgt gctatcgatc aatgttcgcc
    17821 agatccgacc agtgtaagga agaagttctt gaaaaatccg actaccaccc gaaacataga
    17881 tatcttctga gatcgtttga ctatacgaca aaagttcatc gatcgaatgc attacttccg
    17941 cattttcttt cgtgcgaaaa tttggttgcg ttgtcaacac gatgatatgg cgataaggaa
    18001 gtgaaagatc ccccattcct tcataggtct ttcttcccat cactaaaatt cgatccattg
    18061 tatgttcgcg aaagaaacgc atatcatttg gcaatcgcca tggtaacaaa ccatttttgc
    18121 cgatcagacc atttttatct tgtgcccaca tggaaataaa catattatcc gctccttatg
    18181 attttagatg ttttgagata aaaaaagtct caattctttg accagcaagg aattgagaac
    18241 tttaaaaaca ttattttgtt tcacggtgca aagtaacttt gcgttcgcgt gggcaatatt
    18301 tttgtttttc caaacgatca ggattgttac gtttgttttt gcttgttaga tagttacgtt
    18361 ctttacaaga agtacactct aaagtgatgt ttacgcgcat gtttttccct cccattttta
    18421 caagaatcct ctaagatgac tctcagactt gattatctta ccatgatttt ttctagattg
    18481 ctagtgtttt tcacgatttt gttaagtaaa actacttgac gataaataca acggttttac
    18541 ttctcaaaca aaatcttttt ctttttgt
//
LOCUS       DAYYGO010000054        18546 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000054 DAYYGO010000000
VERSION     DAYYGO010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.055"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(34..153)
                     /locus_tag="ACHA5T_001558"
     CDS             complement(34..153)
                     /locus_tag="ACHA5T_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFM8887136.1"
                     /translation="MVFQKPLNNFRYIVYYCHTKIKKNKQKETVTKVLSQSLI"
     gene            332..1993
                     /locus_tag="ACHA5T_001559"
     CDS             332..1993
                     /locus_tag="ACHA5T_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8887137.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2063..2410)
                     /locus_tag="ACHA5T_001560"
     CDS             complement(2063..2410)
                     /locus_tag="ACHA5T_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFM8887138.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSTLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2494..3435
                     /gene="opp3b"
                     /locus_tag="ACHA5T_001561"
     CDS             2494..3435
                     /gene="opp3b"
                     /locus_tag="ACHA5T_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFM8887139.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3436..4479
                     /locus_tag="ACHA5T_001562"
     CDS             3436..4479
                     /locus_tag="ACHA5T_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8887140.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4485..5555
                     /locus_tag="ACHA5T_001563"
     CDS             4485..5555
                     /locus_tag="ACHA5T_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8887141.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5545..6498
                     /locus_tag="ACHA5T_001564"
     CDS             5545..6498
                     /locus_tag="ACHA5T_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8887142.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6510..7520
                     /locus_tag="ACHA5T_001565"
     CDS             6510..7520
                     /locus_tag="ACHA5T_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFM8887143.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7805..8626)
                     /locus_tag="ACHA5T_001566"
     CDS             complement(7805..8626)
                     /locus_tag="ACHA5T_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFM8887144.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8723..9001)
                     /locus_tag="ACHA5T_001567"
     CDS             complement(8723..9001)
                     /locus_tag="ACHA5T_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887145.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9269..11278
                     /gene="metG"
                     /locus_tag="ACHA5T_001568"
     CDS             9269..11278
                     /gene="metG"
                     /locus_tag="ACHA5T_001568"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFM8887146.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11757..12344)
                     /locus_tag="ACHA5T_001569"
     CDS             complement(11757..12344)
                     /locus_tag="ACHA5T_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887147.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12519..13841
                     /locus_tag="ACHA5T_001570"
     CDS             12519..13841
                     /locus_tag="ACHA5T_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8887148.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13996..14772
                     /locus_tag="ACHA5T_001571"
     CDS             13996..14772
                     /locus_tag="ACHA5T_001571"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFM8887149.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14765..15334
                     /gene="rnmV"
                     /locus_tag="ACHA5T_001572"
     CDS             14765..15334
                     /gene="rnmV"
                     /locus_tag="ACHA5T_001572"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFM8887150.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15327..16211
                     /gene="rsmA"
                     /locus_tag="ACHA5T_001573"
     CDS             15327..16211
                     /gene="rsmA"
                     /locus_tag="ACHA5T_001573"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFM8887151.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16662..17291
                     /locus_tag="ACHA5T_001574"
     CDS             16662..17291
                     /locus_tag="ACHA5T_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887152.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17415..18524)
                     /locus_tag="ACHA5T_001575"
     CDS             complement(17415..18524)
                     /locus_tag="ACHA5T_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8887153.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
BASE COUNT         6058 a         3343 c         3778 g         5367 t
ORIGIN      
        1 ataccgagaa actggctttt gaacaccgtt tattcagata agggactgtg acaagacttt
       61 tgtcacagtt tctttttgtt tattcttttt tatctttgta tggcaataat agacaatata
      121 acgaaaatta tttaatggct tttggaagac catggaaaac cttgtacaaa gctatttttt
      181 ctgaaaaatt ccttgaaacc tcacgaaaaa atcattattt tatgaaaaag acaaaaaaaa
      241 gtgttgattt tttaataatt attcattaaa ctcttaaaca acttcagaaa atattaatta
      301 ttctgaaaat tccaataaag gggtacgtaa aatgaaaaag aaatttacat ttggtttggt
      361 tgcggtatgc ggccttgtac ttgctggatg ttatggcggc agttcagcca caagcagtag
      421 ttcagcaaaa agcggttcag cagatggagg aggcgtattt aacttagtcg tgcctcaaga
      481 aatgcctaca gctgacttat cagttgcaac agatacaatc agttttacag cattgaataa
      541 cgtttatgaa ggaatttacc gtcttgatga agacagcaaa ccacagccgg caggagctag
      601 cgagttggca gaagtcagtg aagacggatt aacttacaaa atcaaattac gagaagacgc
      661 aaaatggtca aatggtgatc ctgttacagc agcggattac gtttatggct ggcaacgaac
      721 agtagaccca gctacagctt ctgaatatgc ttacctgtat gcgccagttg aaaatgcaga
      781 agacattaca gctggaaaga aagataaatc agaactaggg atcaaagcag ttggtgacta
      841 tgaattagaa atcaaattga cgaaacaaac accatatttc caatatctgc ttgctttccc
      901 atcatttttc ccacaaaaac aatccgtagt ggaagaaaat ggggatgcct atgcatctgc
      961 aagtgataaa gctgtttata atggaccatt cacattagaa gattttgatg gaccaggaac
     1021 agatacagaa tggacttata agaaaaatga tcattactgg gacaaagata cagtaaaact
     1081 ttctgaaatc aaagtcagtg tagtaaaaga atcttctact gcattgaact tattcaaaga
     1141 tggccaagca gatgacgtga tcttgtctgg tgagttagca cagcaaaacg caaacgatcc
     1201 agcttatact tctgttaaag aagcgcggac aagctatatc gaattcaatc aacgtgaaaa
     1261 gaattcacca ttcaacaatg tgaacctaag aaaagctatt tcttattcta tcgatcgtga
     1321 agctttagta aaacaagtac taggtgacgg atcagttgtc tctacaggat tgatcccatc
     1381 agatatggta aaaagtccaa caacgaacga agactttgca aaagaagctg gaaaattagt
     1441 ttcttatgac aaagataaag caaaagaata ttgggaaaaa gcgaaaaaag aattgggcgt
     1501 tgattcttta gaatttgatt tgatggcttc tgatgatgat tcatcgaaaa aagtaatcga
     1561 atatgttcaa aactcaatcc aagaaaactt ggatggcgta acagtcaaac caacacctgt
     1621 tccattctct gtacgtcttg accgctcaac ttcgggtgat tttgacgcag tactaggcgg
     1681 atgggctgct gactatgcag acccaagcag ctttactgat ttgtttgtca caggtaattc
     1741 atacaaccgt ggtcgctgga gcaatgcaga atatgacgaa gcagttaaag cagcttcatc
     1801 aacagacgct ggcgatgaac aagcccgttg ggatgatttc caaaaagcaa tcaagatcat
     1861 ctctgatgat atgggtgtcg ttccggtgta ccaaaaagca gaaggtcacc tagtgaatga
     1921 aaaaatcaaa ggtatcgttc accatgcggc aggcgcttca tgggattaca aatggacata
     1981 cgttgaagaa taatcgttaa taccgtgagc atgagaaaaa acgaggctgc ggcaaatgtc
     2041 gcaacctcgt tctcatattc acttatccta acaaagaaaa aagcatgaaa atagcagaaa
     2101 gagcaattag tattccggca atgatcagcc aaaagctgta cataccgtag ctggcagacg
     2161 agagcgtgga aaattctggt tttttctttc ggttccttgt tcgtgcaaga tggaccgatc
     2221 gctgaaaatg atcgaagaaa cccgaagaaa agacccataa aaaaccgccg ataatcagaa
     2281 acggcaatga ccataagaat agatcattag ataattgaat gagtgtaaag gaatggttga
     2341 tgagattttt tattaaaata accagaatca gtagaaaagc tactgcgtag gggatagatt
     2401 tttttttcat ttgattgctc ctttattttt ggttcattat ctaataatgt agcgaaacat
     2461 ctgtcagaag tcaaaagcta gggggaaaca ttagtgaata gttatatcaa atatgtatta
     2521 aaacgtgtct ttttcatgat catcacgctt tggttaattg caacaatcac ctttttcttg
     2581 atgcaattgc tgccgggcac gccatatacg aatcaggaac gtttgagtcc tgaaacaatc
     2641 gagatgctaa ataaacaagt cggtttggat aaaccagtta tcgtacaata tgggatttat
     2701 ctttctaatt tgcttcaagg agattttgga atttccttcc aatttaaaaa ccagccagtc
     2761 gcacatttgt tagctggaag aatcggacca tccttgcagt tagggttaca ggcaattatc
     2821 tttggaacag tcctagggac gattttagga acgatctctg ccatgaagca aaatacatgg
     2881 gcagatacat cttctacact cgtagcgata ttaggtcgtt ccattccaaa ctttgtattt
     2941 gctgttttat tacaatatat ctttgcaatc aaattacaag ttttgccaat cgcaaaatgg
     3001 gatggctttg tgtacacaat cttaccaaca attgcgttgg ccatgtcgcc gcttgctgac
     3061 tctgctcggt ttatccgtac ggaaatggtg gaagtactgc atagtgatta tgtggaatta
     3121 gcaaaagcaa aaggattgag ccgttgggaa atcgccttta aacatggtct tcgaaatagt
     3181 ttgattccat tgatgacttt attaggaccg ttagctgtag cattgatgac cggatcactg
     3241 gtggtagaaa atatctttgc tattcctggt atcggggaac aattcgttaa gtcaatcaca
     3301 acaaatgatt atcctacgat catggctgta acgatccttt actcatttat gctgattttt
     3361 gttattttga tcgtggatct attgtacggt ctagttgatc caagaattcg tgtttcagaa
     3421 gggagccgag gataaatgga aatgatacca aagcgttatg aaacagttgc agatatccct
     3481 gcggatgaat ttcttccatt acaaaaaaat accgaagaag aacgtgaaca gattactgca
     3541 ccttctttga attttatcca agattcatgg cgccgtttaa agaaaaacaa agctgccgtt
     3601 atttcaatgg ttttactatt ggtcatcatt ttgatctctg tgatcacaat ctttgtttct
     3661 cctcatgatc caactgccca aaacgtggct tatatcaatt tgcctcctcg tattccggga
     3721 atcaatatcg atggattgaa tggaaaagca atggttggtg gtgaacttgt tgataaatat
     3781 gctcaagcaa acgtcccttc aaacgtaaat ttctttttag gaacagacgg tttgggacgt
     3841 gatgtattaa gccgcttatt tatgggaatg agaatctcgt tgctgatcgc atttattgct
     3901 gcattactag atgtaacgat tggtgtagct tacgggttgg tttctggttt gttaggcgga
     3961 cgtgtcgata acgtgatgca acgttttttg gaaatcttat caggaattcc aaacctcgtt
     4021 gtcatgattt tgatgttggt cgtttttgaa ccaggaattt tttcaatcgt ggctgctatg
     4081 gccatcacaa actggatacc aatggcacgt atcgttcgtg cacaaacaat gaaattaaaa
     4141 gatcaagaat atgttttagc tggattgaca ttaggtgaat caagaatgaa aattgcattc
     4201 aaacatattt tgccgaatat ctctagtgtc attattattc aaatgatgtt tagtattcca
     4261 tctgctattt tcttcgaagc ctttttaagc tttatcggtt taggtcttac gcctccgtca
     4321 gcttcattag gaacgatgtt gagcgaaggg tacaagacat tcctgtatct gccttatcta
     4381 ttatggattc cggcagtaac tttatcggtt atcatgattg gctttaactt acttgctgat
     4441 ggattgcgtg atgcctttga tcctaaaatg aaagagtgaa gacgatgacg aaaattttag
     4501 aagtgaaaga cttagaaatc tcttttgata cgtatgctgg aaaggtacga gcgattcgcg
     4561 gtgtcaattt tcatttgaat aaaggagaaa cattggcgat cgttggggaa tctggaagtg
     4621 gaaaatcagt gacgaccaga agcatcatgc gcttgctgtc aagcaacgcc aatatagatg
     4681 ctggtcaaat cctctttaaa ggtaacgata tcgttggaaa atcagaaaga gagatgcaaa
     4741 aaatccgtgg taaagaaata gcgatgattt tccaagatcc aatgacctct ttagatccga
     4801 cgatgcccat tgggaagcaa gtcgctgagt cccttagaaa gcataataaa gtttcaaaaa
     4861 aagagggaca aaaagctgca ttagaattgc ttaaattagt tggaataccc gaagctgaga
     4921 aacgtatcaa cagttatccg catcagttct caggaggtca gcgtcaacgg atcgtgatcg
     4981 cgattgcact ggtatgttat ccggaaatcc tgattgctga tgaacctaca acagcgcttg
     5041 atgtgactat tcaagcacag attttggagc tgctgaaaga aattcagaca aaaatcgata
     5101 catcaattat ctttatcact catgaccttg gtgtagtagc gaatgtcgct gatcgtgtcg
     5161 ctgtcatgta tggaggaaga atcgttgaag ttggaacatc ggaagaaatt ttctacaacc
     5221 cgcaacatcc ctatacttgg ggactgcttg gttcaatgcc gacattagat agtgcgaacg
     5281 atcgattgta tgctattcct ggttctccac cggatttatt gaacccgcca aaaggagacg
     5341 cattctatcc aagaaatgaa ttcgcgatga aaatcgatgc agaacaagaa ccaccatttt
     5401 ttgaactatc aaaaacacac aaggcagcaa cttggctact tgctccgcag gcacccaaag
     5461 tgacaccgcc agaagaaatc caacgtcgct gggctatctt taaagagaaa caaaaacata
     5521 cttacggtca aggaggaatt gctgatgcca aataaactac ttgaagttaa aggattgaaa
     5581 cagtatttca atgtaggaaa aaagaatgaa gtacatgctg taaatgacat cagctttcac
     5641 atttatgaag gagaaacatt cggtttagtt ggagaatctg gaagcggaaa atccactact
     5701 ggccgtacga tcattcgctt gaatgaacct actgatggtg agatattatt cgacggtcaa
     5761 gatataacga agatcaaaga taaaaaaggt ttgacgaaat tccggcatga tgttcagatg
     5821 atctttcaag atccatacgc ttcgttgaat cctcgtatga aagtcagaga tatcattgcc
     5881 gaaggaattg atgttaatgg actagctaaa tcatctgaag agcgtgcaaa aaaagtcgat
     5941 gatctattga gaactgttgg attgaatcca agccacggta ctcgttatcc tcatgaattt
     6001 tcaggtgggc aacgccagcg tattggtatt gcacgagcat tggcagtaaa accgcgtttt
     6061 atcatttgtg atgaaccaat ttctgcacta gatgtgtcga tccaagccca agtggtcaat
     6121 ttgttgcaag atctccaaaa agaacatcaa ttgacctatc tatttattgc acatgatttg
     6181 tcgatggtta aacatattag tgaccgaatc ggtgtaatgc ataacggctt gttattagag
     6241 atgggaacaa gtgatgaaat ctataatcac ggtgttcatc cttatacaga aagtttgtta
     6301 tctgctattc ctctaccaga tccagaccat gagagaaaac gcagaagaat caaatatcaa
     6361 cctgaaccag atgacggaca agtacgtcaa cttcgtgaaa tcgcaccaga acattttgtc
     6421 tatgcaactg agcaggaagt cccatattac gcaaaaaaac taaaacgaca aaaggaatct
     6481 cttctggttg ctaactagga gggacataga tgctaaaagc ttttaaattc cgtatgtatc
     6541 cgacagaaga acaaaaacag caattgattc gtacttttgg atgtgctcga tttacctata
     6601 accatttgct gaaaaagcgg cagaaaagtt ggcaacaaac aggagtagcc aatttttcgc
     6661 tcacacccgc tacattaaaa aaagaatacc cattcttaaa agaagtagac agtcttgctt
     6721 tagcaaatgc acagcttaat ttggatcggg cttttcgcaa ttatttcaag ggacgtgcca
     6781 gcttcccaaa gcttaaaacg aaaaaaagta tgtggcaatc ttatacgacc aacaaccaaa
     6841 cccgtacgat ctatctggaa aatggctact taaagttacc aaaacaaaaa gaactaatca
     6901 agataaatca gcatcggcca gttgaaggat cgattcgctc cgctacgatt tctgctcgat
     6961 ataatgaaga attttatgtc gcactacttt gtgatgtttc gccagtaaaa aaagaatctt
     7021 tagctaaatg gatcgggatc gcctatcatc cgaagacatt gatccaaaca tctcgacctc
     7081 tagaagtaac tttaccaaaa tttcaccaga cagaagaaaa gctgcaacat gcacagcgga
     7141 aattgaatgt gaaagtccgc tccgctcacc atagaaagat caggttagat caggcaagca
     7201 attatcagaa acaaaagaga aaagtaatgg atctttatct gaaacaaaag aaccagcgag
     7261 aagattatct tgaacaatta tctgggaaat tggtcaaaca atatgactac ttatttgtag
     7321 aatcttttcc aaaagaagag gcacatgctg atttttctat tcacgactgg cataaattaa
     7381 taacaaaatt gcgttataag tcccaatggt ataataaaaa atttcttttg atcaataccg
     7441 acggagcaga agaaagcaat tccgtaagaa agagccaagt agtagaaaaa atgggcagac
     7501 attcggttat caagggataa aaaatgaatg aaactgatag gagcgttagg gttagccttg
     7561 ttaataaaaa gtcttaatga cttttgtttc taggaatttt tcttttgaag aaaagctcac
     7621 taattatgac aaaccaataa aacaatagca gaaaaagttc gtacgagctt tttctgctat
     7681 tgttaatttt ttattcagaa agtttacgaa tacatcaaaa ttctgaaaca aaaaacgtaa
     7741 ctcacaaaga gttgtttttg cgttttttct tgaatgatta ggtctgaatc ttttgctcac
     7801 aaccttattc atggtgcccc ggcttgagct gatagatttt tgaaggacgg ccgattccta
     7861 caggacgttc accgatctca tcgaaatatt ggagcatagc tttcttaaaa ttggaatgat
     7921 cgatcgtttt ataatcgatc cctaagaatt tagcaaatac ttttcgagct tccgtaatcg
     7981 tgaaatcttt acctaaaact tgtagaactt gcggctcgtg ttccatttta ttgactacac
     8041 ggttgaatgc tttcaagatg atttgactat ggtcaaaagc caaagtatct ttgccagaag
     8101 actctccagt gactaaatca agcgagatag ccacttctcc gctagataaa tggatcttgt
     8161 tactatgacg ttctagtgtg aaccagcgta cttccttggc atcatctccg gcgattagcg
     8221 gttcttctcc aataaaggct aagtagctga ctgtgacgac ccagcctcgc ggatctcgat
     8281 taggcgtact gaatgtatgc agttgctcaa tattttcttt tgaaataacc acaccagttt
     8341 cttcctttgt ttctcttaag acgctttctc cagttgactc atccttttgg acaaagccac
     8401 caggcaatgc ccatgaattt cgataaggat gcccttttct ttggatcaac aaaactttca
     8461 gctgatcttc ttctttgtta tagcacatca gtacgatatc gacagtcaaa gagggcttgt
     8521 catattctgg acgttcctgt ttttgatacc agttaagaaa ttcttcttca cttgctttat
     8581 gttcataata taatttttct tcttctttag aagcaaaacg gttcatctga ccacctccat
     8641 tgacagtaaa agtacttttt accttattgt agcagactct gaaaaaaaac caaggattta
     8701 cttgaaaaat ctaggttatc atttaaatat gaaaaataaa tggaagtatg agattgaata
     8761 aaaagaaaag cagataaaac ggcaatactt tataccgctc ttcacgaatg gcaaagaagc
     8821 cacaaataac gatatatacg tcaatgataa aacgcaaaat ccaagctgct gtcataaaac
     8881 tgttcaaatg cccatggagg ttaaaataac catacatacg aatataaaaa tagctgaata
     8941 ttaaatgtgc taatgaaaga aaagcaatac taagtgtaaa aaaaagatgt ttcttctgca
     9001 tgattgttcc agtcccttac taagatagat agtctttatt ataccacgtg tgaggggaga
     9061 aataaaaagc cgttttttag tagatatgaa acaaaacaaa aacccgcatt tattcaacct
     9121 attcatcctt ttaaaggata gattgaacgt agatagaagt cgtttatcca gtctttttca
     9181 ttttgaaaaa aagctaacag catgcttttt ttatggtata ctagcaagga aacgaattcg
     9241 catcatgcga taaaggagga agagccccgt ggcagaaaag gaaacttttt atatcactac
     9301 gccaatctat tatccaagcg gcaaacttca tatcggcaac tcgtatacta cgattgcatg
     9361 tgacgcaatt gctcgttata aacggttgat gggctttgat gtgttttatc tcactggagt
     9421 ggatgaacat ggtcaaaaaa tcgaaacaaa agcagaagaa ctaggtgtaa aaccgcaaga
     9481 atatgtagat aaaatggcgg ctgatgtcaa gaaattatgg aaaacgcttg atatcagtta
     9541 tgataaattc attcgcacga cagatgatta ccataaagca gctgttcaaa aaatatttga
     9601 tcgtttatta gaacagggag atatttactt aggtgaatat gaaggctggt actctgtttc
     9661 tgacgaagaa ttctttacag aaacacaact tgcagaagtg tacaaagatg acgaaggtaa
     9721 cgtgatcggt ggtaaagcgc caagtggaca cgaagtagaa ttggtaaaag aagaatcata
     9781 cttcttccgc atgagcaaat acgcagaccg tctattggaa tactatgaag aacatcccga
     9841 attcatccag ccagaatctc gtaaaaacga aatgatcaac aactttatca aaccaggatt
     9901 agaggatctt gcagtttcac gaacaacttt tacttgggga gtaccagtca agagtgatcc
     9961 gaaacatgtg gtgtatgtat ggatcgatgc attatccaat tatattactg cactaggata
    10021 tggctccgaa gatgaaagtc tattcgaaaa atactggcct gcagatgttc agatggtcgg
    10081 taaagaaatc gttcgctttc atacgatcta ttggccaatc atgctgatgg cccttgattt
    10141 gcctctacct aaaaaagtat ttgggcatgg ctggctattg atgaaagacg gaaaaatgtc
    10201 taaatcaaaa ggaaatgtcg tttatcctga aatgcttgtg gaacgttatg gtttagatgc
    10261 actccgttat tatttgcttc gtgctgttcc gtttgggtca gatggtgtct ttacgccaga
    10321 agattttgtt tcccgtgtca attatgatct ggcaaatgat ctagggaact tattgaatcg
    10381 aacgattgct atgatcaaca aatattgtga tggactcgtt cctgactatg cctcacttgt
    10441 cactccattt gatagtgaac tgtctacgac ggctgctaac gttgtcggac gttaccatga
    10501 tgcaatggaa aaaatggaat tcaatacagc attggctgaa atctgggtgc tgatttctcg
    10561 tgcaaacaaa tacattgatg aaacagaacc atgggtatta gcaaaagacg aagaaaagaa
    10621 agctgaattg aatagtgtca tgatccattt agctgaatct ttgcgtatcg cagcgatatt
    10681 acttcagcca atcatgacag aaacgccaga gaagatcttc aatcaactag gattggactc
    10741 tgaaacaatg aatcttgaag gattacactt tggcgaattt ccttcaggaa caaaagtagt
    10801 tgcaaaaggt acaccaatct tcccacgttt agatatggaa gaagaagttg cttttattca
    10861 agaaaaaatg tctgaaggta ctcaaacaaa cgaagataca gtcaaatggg atccagaaga
    10921 aacagaactt gtttcaacga aagaaaaaca aatcaaattt gatgtatttg aaaaagtaga
    10981 actaaaagta gcagaagtca tcaactgtca aaaagtagaa ggggcagata agttgctgca
    11041 attccgacta gatgctggag acagccaaga ccgtcaaatc ttgtcaggaa tcgctgaatt
    11101 ttatcctgat ccaagcgaat tgatcgggaa aaaagtcgtc attgtagcaa atctgaaacc
    11161 acgaaagatg cgtggacaaa tcagtcaagg aatgatcctt tctgcagaag caccagatgg
    11221 ctctttacaa gtaatcgaag caccaaaatc catgccaaat ggctcagaaa tcgcatagaa
    11281 attcaaaggg ggtatgacac tagttgtgtc ataccccctt tttccgaaat ttgagaagca
    11341 attttcgtaa attccttctt attacttgaa gccaatcata taatacagaa acagagaact
    11401 atccaaaata aatcctagaa aaagaagtac gagcttcgct gatttttgtt aatttagaat
    11461 aatactaata ggaatcgtaa aaacagaaaa aaagcataat gataaagggt cgggcagatt
    11521 ttaattgatt catctggtac tcttttttat tgtccttaca ttcataaagt ttgaatgcaa
    11581 tgactttatt acatctatat ttatatctaa cagtagcgtt gatgtgttaa ttataagttc
    11641 tatcacttta tgttcaatta gtttgtgctc ttatatgtgt gattgaaaat atagataaaa
    11701 aaacaatcat cggctttgac ttcgatgatt gttttcttta ttttgtaaaa atcctatcac
    11761 ttgattttat ggtaaccttt tatttttgtc ttcaaacggc ttggtaagag agcagtaagc
    11821 agtgcaaatg ggatcgaaaa gacataccag aaaatcgtac gtccgatacc ataatgttga
    11881 tagagctttg tcaacatcac gtgtaacaag atcaacccta aaagcaagga gccagctata
    11941 aaccaatgat tatcccaatg catgacttca ctaagtgcca tcactccaaa aggatagaaa
    12001 taaaaataga tcccgactaa ggtcatccgc cagttctttg taaatgttcc gatattcaat
    12061 aaaaagaaaa tcgctgaaag caaaataccg ataggcggaa aaagcccaac caaaaaggaa
    12121 tacaacatcc cccacaagat catcacagga cctttgatca atgcaacgat tgcgatgaac
    12181 aaagtcagca atattagttt ttgtgataac gttgcaacag tcattaatcc aaataaaatt
    12241 aatgtgaacc aatgccaatg ggactgctgt tgttctttac gggaaaaatc gacccggttg
    12301 gataaataat ctgctaattt cggataagaa cgcttaaaat ccataaactg ttcctccttc
    12361 gtggaaaagg taggtttagt atacgaaaaa taaaaaagaa atacatccgt cttaagaggg
    12421 agtttttcac tgaaagtgga gaaaaaagaa tggtaaatta aaatattctc agttatactg
    12481 aaaattatac caaatttcaa agaagaaaga ggatgggaat gaaaggcaaa ctaaaacgag
    12541 aaatcaatct gtttggcgca ttagctaccg ttatgggaac cgtgatcggt gcaggcgtct
    12601 tcttcaaaac tgctgctgtt actgctagta ctcagtctac aagtttgaca ttgctagctt
    12661 ggcttttggg tggtttctta acgatctgtg ctggattgac agtggctgaa ttagctacag
    12721 caatcccgga aactggtgga gctgtaaaat atattgaagc tgcgtatgga aaattgccca
    12781 gctttttatt aggatgggcg caaagcttga tttactttcc tgctaatatt gctgcattat
    12841 cgattatctt tgcgacacaa ttgacgaact tattacaatt atctaccgat tatcttttgt
    12901 tgattgcagt gatcaccgct gtttctgtga cagggttgaa tttgttaggg acaaaagtcg
    12961 gtgcttctgt acaatcagtg acgttgattg ttaaattaat accaattgct gtgattgtca
    13021 tatggggatt attgacccca ggacaaggaa ctgtccagtt atttccaatc gaagctggaa
    13081 aagatgttac ttttgtcgaa ggattgagca gtgcgttgtt agcaacgttg tttgcttatg
    13141 acggctggtt aggtgtcggt gcaatggcag gggaaatgaa acggccggaa aaagatttgc
    13201 ctaaagcaat tattcttggt ttgagttttg taacagtcgt ttatctattg atcaattttg
    13261 tttttttgaa aactttgccg attgatcatc ttgccggtaa tttgaatgca gcttctgaag
    13321 catcagacgt catttttgga ggaatcggtg gaaagctggt cactattggg atcttgatct
    13381 cggtatacgg tgcattgaac ggttatacac taactgggat ccgcgtgcct tatgcgatgg
    13441 ctttagaaga cgagctacct ttcagtaaac agctgacaaa gctttcaaag aagtttacgg
    13501 ttccttatgt tcctgcggtt ttccagttag cagtagcttg tattatgatg agccttggtt
    13561 ctttcgactt tttaacagac atgttgattt ttgtgatgtg gctattcagt ttactgattt
    13621 ttatcggtgt ctttatttta agaaaaaaag cgccggaatt gccacgtcca tataaagtac
    13681 cactttatcc aatcgttccg attattgcga ttctcggtgc tattttcatt ttaggtatga
    13741 cgatgatgac gcaaacgaaa ttagcattga tcggtatagg tgttaccttg ataggtatcc
    13801 cagtctatta tcaaaaaaag aaaaaacgct cagaagagta aaatatttca acaacagaca
    13861 gaaaaagtac gaactaggtc ctctagatct tcgtgctttt tctatttcaa taaaacaagt
    13921 tcttctattt tgatttttgc tacttctgca agtagtgctt atgtgctaaa gtaagaatga
    13981 aaaggagaga taaacatgat atttgattct catacccatt taaatgctga acagttcaat
    14041 gaagatattc cagaaacgat tcagcgagca caagaaatgg gggtaacgaa aatggcggtc
    14101 gtgggcttcg atacgccaac gattgaaaag tctttgcaat taagccatga ctatccaaat
    14161 atatacagta ttatcggctg gcatccgaca gaggctggca gttatacgaa agaaatcgaa
    14221 aataaattac aagaacaatt gactttacca aaggttgtag cattagggga aattggcttg
    14281 gattatcatt ggatggaaga cccgaaagaa gtccaagata aagtatttcg cagacagatt
    14341 gctatcgcaa aagaaatgaa cctaccaatc agtattcata ctagagaagc acttgaagac
    14401 acgtatcaaa tcctaaaaga agaagatgtt cgagatatcg gtggaatcat gcatagtttc
    14461 agtggtgatt atgaatgggc aaaacgattt cttgacttag ggatgcatat ttcatttagc
    14521 ggtgtcgtca ctttcaaaaa agcattagac gtccaagagg ctgcagcaaa tgtaccaatc
    14581 gaacgattac ttgtggaaac agatgcccct tatctggcac cagttccttt tagaggaaag
    14641 cgaaatgaac caggctatac tcgttataca gtagaaaaga ttgctgaact tcggaatctt
    14701 ccgacagaag aagtggctgc ccaaacatgg acaaatgcgc accgattatt tggtctaagc
    14761 gaacatggat aagatgaaga tccaagagat tatagtcgtt gaaggaaaag acgatacaag
    14821 aaggctgcgg gaagtagttg atgcagatac gatagaaaca atagggtctg caatcaatga
    14881 agagatcctg atgcaaattg aacatgccca agagactcgt ggagtgatcg tttttaccga
    14941 tcctgatttt tcaggagaaa aaataagaaa aatcattatg gaagctgttc ctcaggcaaa
    15001 acatgctttt ttgccgcgca cacaagctaa accaaagaaa aagggaagcc ttggtgtcga
    15061 gcatgcaagt gatgctgcta ttttagaagc attgaaaaag gtcgtgacgc cagacatttc
    15121 ccaaacggct gtttctgaaa tcactagaca agacctttta gactatggtt taatagctgg
    15181 ggcacaagca aaagaaaaac gagaaaaact aggtgacgag ttgcgcatcg gttacacgaa
    15241 cggcaaacaa ttggaaaaac gtttgaagat gtttcgtatt acgccgaaag aactcgccca
    15301 agcaatgaaa atagtggagg aacaacatga gtgaatatcg tgaaattgcg acaccctcta
    15361 gaacaaaaga gattttattg aaacatggtt tttcatttaa aaaaagttta ggacaaaatt
    15421 ttctgactga accaaatatt ttaaggaaaa tcgtagaaac tgcgcaaata gatgaacata
    15481 cgaacgtgat cgaagttgga ccaggaatcg gtgcattgac ggaacaatta gccaagcatg
    15541 caaaacaagt cgtagcattt gagatcgatg atcgtttgat ccctgtattg gctgatacga
    15601 tgcaacccta tgacaatgtg actattgtac atcaagatat tttaaagacc gatttgtcaa
    15661 cagctgttag agaaacattt catgaggagc ttccgttaaa agtcgtagcg aatttacctt
    15721 actatattac gacaccgatc atgatgcatt ttcttgaatc agatttggtt gtggatgaac
    15781 tagtcgtcat gatgcaaaaa gaagtagctg atcgtatctc tgctgaacca ggaacgaaag
    15841 cttatggttc attatcgatt gctgttcaat attatatgga agccagttta gcattcatcg
    15901 taccaaaaac agtatttgtc cctcagccaa atgtagattc agcgattttg aaactgacaa
    15961 gaagagatat accggcagtc gaagtgacag acgaaaaagc atttttccga ttgacaaaag
    16021 cggcatttca gcaaagaaga aaaacgcttt ggaataactt gcagcattcg tatggcaaag
    16081 atgaccagac aaaagcatgg ttagcaaaaa gcttggaaac agctggaatc gatccaaaac
    16141 gtcgtgggga gactttgtca ttacaagaat tcgccgcatt aagcaatgcg atgtcagaaa
    16201 atatgcagta aaaaaagagg ttgtgacaga agttgtgaaa aagctgtcct gcatcaagta
    16261 ataagaacag ccaaacaaaa atagttcctc atgtttttcg tttgtttggg cttattaccg
    16321 cagatgcaag cttttgaaca ccgtttaaga aagaggttgt gggcctgccg tttaactcta
    16381 agtcacaagg aacaatttca aaaaacagct tctcatgttt ttgagttagt tggtcttatt
    16441 acttgatgca agcttttgac cactattatg ccgaacaagg agatgtgaca caaattttgt
    16501 cacatctcct atttttgttt cttgatcaaa aaattcttat acttgatact cacactcatt
    16561 ttgtatggat ttagaaaaga atatgtgtct tgacagttat tagtaagtga gttatactaa
    16621 gtcgacattt atgtgtcaag acatattagg agagagaaaa aatgagaaaa aattcagttc
    16681 gaaaactaac gatttctgct ttattgattg ggatgggggt cattatccca atggtcatgc
    16741 ctaaaatcat gattccacca gcttccttta ctttggcgtc tcatgtgcca ttatttattg
    16801 cgatgttttt tacaccagga gttgcagtgg ctgttgcgct aggtacgaca tttggtttct
    16861 ttttaacaac gccggttatc atcgcattgc gagcattgtc acatcttgtg tttgcgttga
    16921 ttggggcttg gtacttgcaa aagcatccga gtatcgtttt aaaaaatgga cagtttactt
    16981 ttgctaattg gcgtttccaa gggttcaatt ttgtgatcgg tgtgattcat tctttagcag
    17041 aaatgctagt agtcagcatc ttttacttta ttggtaatat gcctgaaaca tattattctc
    17101 aaggatattt ttatacagta ttcatcttga tggggattgg tgggcttatc cacagtttag
    17161 ttgattataa tattgcttat tttatagcag gaacactcag caaatatttt gatatccccg
    17221 tatttacggc agcaaaaaaa gaaaatcgga aagagtcgat tcaagggtac gcgaagaaaa
    17281 cgacatatta gacatgatct ctcaaaaaaa aaactctcat ctagtaatgg ataataaatc
    17341 ctctaagatg agagtttttt gtcctaaaaa gagctgcacc aacatcttct ggcacagctc
    17401 taacaggggg caatttatgg aagtttgatg acttgttctg tttctttatc aaagaagtgg
    17461 cctttgctga tgttgaaggc aaggtcgact atttcgcctg gtttatggaa atctcttgca
    17521 tctacttttg agatgaactc agtagaacca gtttttgtgt aaagcatcgt ttcagctcca
    17581 agtaattcgg aaacgacaac ttctgatttc actacggctt caggtgaagc ttcaagggct
    17641 acttgttcac tgtggatgtc ttctggacga ataccgaaga taacttcttt tccttcataa
    17701 cctttttcaa ctaatacttt gttttttcct tcaggaatac gaagcttaag cccatgtcca
    17761 tctgtgataa caccattttt caatgtcaca ttaaagaagt tcatggcagg agatccaata
    17821 aagccggcaa cgaacatatt gacgggtgta tcataaactt ctttcggtgt accgatttgt
    17881 tggataaatc cgtctttcat gataacgatt cggtcagcca tagtcattgc ttctgtctga
    17941 tcgtgagtaa catagatggt cgttgtttca agacgctggt gaagtttggc aatctcagca
    18001 cgcatggcta cacgtaattt agcatctaaa tttgataatg gctcatccat taagaagact
    18061 tttgcatccc gaacgattgc ccgtcccaat gccacacgtt ggcgttgtcc accagataaa
    18121 gcagctggtt ttcgtttcaa gtattctgtc agacctaaga tctctgctgc attgtccaca
    18181 cgttttttga tgtctgcttt atcatattta cgtaatttca agccaaaagc catattatca
    18241 aaaacagtca tatgaggata caatgcgtag ttttggaaaa ccatggcaat atcacggtct
    18301 ttaggtgcca cgtcattcat gacttttccg ccgatattta gttctccttc agagatatct
    18361 tccagtcccg cgatcattcg caaagtagtt gactttccac aaccggaagg accaacaaaa
    18421 acgataaatt cacggtcttt gattgataga ttaaaatcag ttacagaata attttctgca
    18481 ttgtcatatt ttttatatac gtttttcaga gccatttcta ccataatacg ttccacactt
    18541 tctttt
//
LOCUS       DAYYGO010000055        18198 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000055 DAYYGO010000000
VERSION     DAYYGO010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.056"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(17..841)
                     /locus_tag="ACHA5T_001576"
     CDS             complement(17..841)
                     /locus_tag="ACHA5T_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFM8887154.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(814..1494)
                     /locus_tag="ACHA5T_001577"
     CDS             complement(814..1494)
                     /locus_tag="ACHA5T_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM8887155.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(1517..2455)
                     /locus_tag="ACHA5T_001578"
     CDS             complement(1517..2455)
                     /locus_tag="ACHA5T_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFM8887156.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(2700..3551)
                     /gene="ispE"
                     /locus_tag="ACHA5T_001579"
     CDS             complement(2700..3551)
                     /gene="ispE"
                     /locus_tag="ACHA5T_001579"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFM8887157.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(3705..6278)
                     /gene="mprF"
                     /locus_tag="ACHA5T_001580"
     CDS             complement(3705..6278)
                     /gene="mprF"
                     /locus_tag="ACHA5T_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFM8887158.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            6419..7225
                     /gene="proC"
                     /locus_tag="ACHA5T_001581"
     CDS             6419..7225
                     /gene="proC"
                     /locus_tag="ACHA5T_001581"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFM8887159.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(7304..8860)
                     /gene="rny"
                     /locus_tag="ACHA5T_001582"
     CDS             complement(7304..8860)
                     /gene="rny"
                     /locus_tag="ACHA5T_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFM8887160.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(9271..10320)
                     /gene="recA"
                     /locus_tag="ACHA5T_001583"
     CDS             complement(9271..10320)
                     /gene="recA"
                     /locus_tag="ACHA5T_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFM8887161.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(10429..11670)
                     /locus_tag="ACHA5T_001584"
     CDS             complement(10429..11670)
                     /locus_tag="ACHA5T_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFM8887162.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(11910..12488)
                     /gene="pgsA"
                     /locus_tag="ACHA5T_001585"
     CDS             complement(11910..12488)
                     /gene="pgsA"
                     /locus_tag="ACHA5T_001585"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFM8887163.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(12697..13620)
                     /locus_tag="ACHA5T_001586"
     CDS             complement(12697..13620)
                     /locus_tag="ACHA5T_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFM8887164.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(14358..15644)
                     /locus_tag="ACHA5T_001587"
     CDS             complement(14358..15644)
                     /locus_tag="ACHA5T_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFM8887165.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(15637..16905)
                     /locus_tag="ACHA5T_001588"
     CDS             complement(15637..16905)
                     /locus_tag="ACHA5T_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFM8887166.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            17157..17618
                     /gene="mscL"
                     /locus_tag="ACHA5T_001589"
     CDS             17157..17618
                     /gene="mscL"
                     /locus_tag="ACHA5T_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFM8887167.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(17677..18123)
                     /locus_tag="ACHA5T_001590"
     CDS             complement(17677..18123)
                     /locus_tag="ACHA5T_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8887168.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
BASE COUNT         5190 a         3981 c         3181 g         5846 t
ORIGIN      
        1 ttcttttctt tataatttag ttattttttc ttctgaaggc aaccaatatt cgtttgatga
       61 tatttacaat caggaacaaa gcaataaaga ctaatgtgat tgttgctcct ggaggtgtat
      121 ctagataaaa cgagctagtt agtcctgaca gcatccctag aaatcctaca caaacactga
      181 tagcaatcac gacattgaag cttttgccta atcgcatacc gattgctgcg ggaagaacca
      241 tgattgcgga tatcaaaagt gcaccggcaa tcgggatcat cacagcaata gccacaccag
      301 taatcacatt gaacatcatc gacatcaaat gaacgggtag cccatccacg tgggcggtat
      361 cttcatcaaa agtcagaaca tacatcggcc gtttgaataa aaagaacaat ataccgatgg
      421 caacgaataa gattcctaaa aagatgactt gctcccatgt aatggtcacg atcgaaccaa
      481 acagatagga ttggatactt gttgctgaat tccctccgct caaattcatc aatactaacg
      541 ctagcgccaa accaccagcc attaaaatgg caatggagat ttctgaatag gttcggtaga
      601 tcgttcgcag atactccaat atcacagctg ctaatacaac gactagcatg gtcgtaagtg
      661 taggattaag gttcaaaaag aatcctaacg ctacccctgc taaagagaca tgagaaagcg
      721 tatcagccat cagcgattgt cttctgatca ctaaaaacac acctagcatc ggggcgattc
      781 cggcaatgaa gatagcggca agaaacgctc ttctcatgaa ttcatatgaa agcaacgcca
      841 ttgagaatcc tcctttctca ccagtcggat ctgtctgtcc gcatactgtt tgatatcttc
      901 atgatcatgg gtgatcatta gaactgattt cccatggaca tgcgcactat gtcggagaag
      961 acgataaaat tcatttctgg attcttcatc catacctgtt gttggttcat caagaacaaa
     1021 taagtcaggg tcagtagcaa aaatacgcgc taaactgatt cgctgctttt gtccgccaga
     1081 caattcacct attcgccgat ttcgcatatc ccacatccct acagcttcta aagcacgctg
     1141 tacatgttga tgatcttttt cagagagacg cttgaaccag cggcctcttg gatatcttcc
     1201 tgaacggact aattcgatca ccgtactagg aaaacctgca ttgaaagatg caacttgttg
     1261 aggaatataa ccgatactta atttttttcc ttcttgattt ttctcagcaa tctttatcat
     1321 tccttttgaa ggtttcagta atcctaacga tgctttcacc aaggttgact tggctgctcc
     1381 attttctcca gttaaaatga caaattcacc cgcatctaca tgataagaga catcttctag
     1441 tacaggttca tcatcatagt aaaaggtcaa atcagatatt tcgatataac gcaccttaca
     1501 acctccattc taaaacttaa ttgatacttt cttttaaagc tttcagattt ttttccatga
     1561 cagaaatata attttcgcct gctttgattt gttcattagt caagctttct aaaggattca
     1621 acacttctag cttcactcct gtttcattcg acaatgtctc agctaccttc gaagaggcat
     1681 tttcttcaaa ataaatcact ttgatgttgt ttttcttcac aaattctttt agctcagcta
     1741 agcgacttgg tgtgggttcc tgatctggtg aaacgcccga aatcgctact tgttttaagt
     1801 cgtattgatt cgctaaataa gcaaaagcag catgctgagt gacaaatgtg cggtttgttg
     1861 catcttttag ctcatcggag tatttttgat ccagtgcttc aagctttttg atataagcat
     1921 cggcattctt cgtaaaagtt tcctttttat ctggatattt ttcgcctaat tcttcagcaa
     1981 tcgttctgac ttcttttata gctaataccg gatcgagcca aacatgtgga tcgtattcat
     2041 gtgaatgacc atgcccttct tctgattctt catgttcgtc tgcttcttgc gattctaaac
     2101 ggtcgattcc tttagctgct tcgatcatca aggtctgttt gctatccact gaatccttta
     2161 acgaatccac aaaaaattcc atgtctgaac tattatacac gaaaacatct gcatcggaaa
     2221 tttctgctct ttcttttgca cttggttcga aatcatgcgg ctccgttcca gcagggatca
     2281 aaagtttcac gtttccttca tcccctacga tctcttttgt aaaatcatac atcggataaa
     2341 aagtcgtaac gatcgtcaaa tcctcttttt tgtctgcttc cttgttcgta tttccacatg
     2401 ctgcaaacag taaagcacct atcattaccg tagctgtcaa aagatatttt ttcatttata
     2461 tgctcctttc aatgaacaaa tcggaatttt acgatttatt aacgtgaact aatgtatcaa
     2521 aataaaaaaa cacagtcaat agaaatccat caatttctga tttaagcatg tatattcttg
     2581 attaaatcgt aaaatggatg agttgctgct catatctagc catttcacta caataaaaaa
     2641 ctgaagaata ccattttagg attcctcagt ttttcatcac ctattttact tcttatattt
     2701 tattgcaggg ttcgtactaa ataaacttcc tcacaaaaac cttttaaccc attatatact
     2761 ctctgcgctc ttgaataatg ttgacacaag gcaaaaacgg ttggtccgct tccactcatc
     2821 aatgcagcat ctgctccgta cttgatcata cgctctttga tttgatggat gactggatgc
     2881 cgctttcccg ttatatcttc taaactgttt cccatttttt tgatcatctg ctgataatct
     2941 tgctctcgga tggcttcagc aagtccgtgg atgtcttgat gttggatggc ttgcgtatct
     3001 acttctctaa acactgtacg tgtagagaca ctcattcttg gtttgaccaa cacaacccag
     3061 cactgtggca tagaaggtaa tacttggatt tcttcccctt tgccaccgac aaatgccgta
     3121 cttccgtaga cacagtaagg aacatctgta ccgacttcca aaccgatttc tgccagttct
     3181 tctaacgaac agcccagatt ccataattgg ttgatccctc ttagagcagc tgcacagtct
     3241 gtacttcctc cgcctaatcc agctgctact ggaatattct tcttaacaaa gatcttgaca
     3301 ccggttttga tattgtattt attttttagc aataaagcag cttgataaac attgttccgt
     3361 tcatctaccg gtaaaaatgc tttatttgtt tctacaatga ttttgtcttc tgttatttct
     3421 tcaaaaaata gatgatctgc caagtctacg ctcgacatca ccatttccaa ctcatgatac
     3481 ccatcttgcc gtctatataa tacatccagt cctaaattta tcttggctgg cgctttttca
     3541 atcaattcca tcctctcacc cattcgaaaa gtctaagtcg caagtattct atcataggga
     3601 aaaataaaag tatagcttat gtctgcctag tggttgattt tgtcatcgtt tcaacagctt
     3661 tcacagaatt ttctcttcat aatccaaaca agttctagtg attcttataa ttctgtgagc
     3721 acttcttctg ccaaactcgg aacatttcct ttttcttttc ctatcagact gagtagctgc
     3781 atggtagcaa atccgaaatt acttcctttc ctataagcaa aataacgtcc ttcccaatga
     3841 tcgacatatt ttaatttctt ttcataagca gactggaagg aatagaaaaa attaccataa
     3901 ttatatacga tatgcatgac tttttcttga aagaaagaaa aatctgtttc tccaacatta
     3961 gctaatggag ctaagcctag attggcttcc gagtagccca ttttctcgta tacaataaac
     4021 agattggcta acagatagtc agctaattcc tcagattctt taggtaagac ccgcaacaaa
     4081 tcatatcccg cctgcttctt cgtcatcggc tgagcggtga tgaagccaac gactttttga
     4141 tctttacgga caataccgac accgctagat aatacatatg atttatcaaa tcgtcccaca
     4201 gagaagtatt tttctcgtgc accttgaagc catttttgtg aaacttcatt tacagcttga
     4261 tgtaattctt ccggcagttg atcgtaccaa gtaaaatggt atccttcttt catcaaacgt
     4321 tgatattcaa gttgctgact cagccgagac aagtcttctg ttgctagatc aacaatccct
     4381 gcttcaccta ctttcgtgaa gtgaaaaccc aattcatgaa gcatcactac gtagctttct
     4441 gatatcttat aaaacgcaag ctgatagcct aaacaatctg cttgtttcat aaactgattc
     4501 gttgctgccc gcatcatttt aggattgcca actggatctc ctaaaacaaa gcatttgttt
     4561 cctttgatct gataaccaaa aacgacttga tcttggtcgt cttcttggta ataaaaataa
     4621 ctgtaacctg acaagcgaag ctgatggctt gtagctgtgc ctccgtatgt ttccaataaa
     4681 cggtcaaaac ggtctttttc gtactcactg ccaagcttcg tagtatcttc agctaaatat
     4741 tgataaagcg ccactaaagc tagcaatgag atgcccaaac caataagccc tgagaaccac
     4801 acatcatctg aaggaaacaa aataaaatga tttcctgtta ttcgaccgac taaaaagcgt
     4861 ccgctgtgat agcctgcaat tgcgtaaacg ataaacaaaa agccgaacaa gctagcatca
     4921 aagattatag ctccccaaga ataaacgaat tttgttcgat aaaattcttt tctggataac
     4981 cacacagcta acagaatcaa gacatatacc aatatcaact gccaagataa cgtacgtgtg
     5041 attgtattta aaataccaaa agatagtaaa gcaatcgtag gcagaaatgc ctttttgact
     5101 tttactgaaa gtgctctagc aaggccgagt aataaaaagc caaccaatag attcactgtc
     5161 tgatctaaaa aattgaatga gaacggcaat aatacttgaa atagccgact gattgcagac
     5221 agattagtga ttgttgatag aagtaccatc atgatccccg caaaatagac agcacttacg
     5281 agcacgatat gcgcaacctt ttggaaaaac agtcgcggaa tgttatctaa aaatcgattg
     5341 attttgactc ctgtatgtgt gatgaacaat agaagtcccg tcataaaagg tagaacataa
     5401 taaaacaacc gataatataa tagccaaact actgctacat cttgccgaac aggcaattga
     5461 ctcattccta agatcatcaa aacgtcaaaa gtccccattc cacctgggac catcgtcagc
     5521 attcctatca atgtagcaat catgaacata ggatagatcg aaagcatcga aatatctatt
     5581 ctcatcaact tgccgatgat caaaaatacc gcaagggctc ccgtccattg tccaaaggat
     5641 gccagtagca gaccaagcaa gccttttgga taaaactcga agaacaatgt ctttctcttt
     5701 agataagcaa aaaagaaaat gactggtgct aaaaaactac cgccgaaaag ccagatccaa
     5761 tagcttcgaa aaatactttc tgactgaata aaaaagacat caataaatgt aagaaacgac
     5821 catacagaca aaccagtcag cataaatagt gcgacttttg atacagtggc taaaaccatt
     5881 tttctatttg tctgctttcc ataaaaacta gcccgcagac ttgctcctac cacaccacca
     5941 aaaccggcga gattgttgat cgtgtttgtt acccacgcag caagaagaaa ctcttttctt
     6001 tccatttttg gttttccttg tttttctaag actctgatcg tcacccaatc atacaacaac
     6061 atcggagtga cacccagcag tccagccaga atcatgagca ggatcgtcat gcggctttgt
     6121 tgtcccatcc tttgccaaat atcttgccaa ctcatgccat tagcaatatt tgcgacttga
     6181 tttgccacaa agactaacac ggagccgaaa aaaatcagtt ttaaaatcag actatgttcc
     6241 tttatccatt gcagcatcaa gtatcgttta tttttcacat tttcccattc ctaacttgta
     6301 tacttttgtg tcatacccca atgttatctt atttatttta atttttccac tttagacctg
     6361 ttctttgctt gcgatgatag aaaaagaaag atatcctaat gctaaaggag aggataacat
     6421 gaaagtagga tttatcggca cagggaatat ggcacaagct attattgatg gtttattaaa
     6481 aaaaggaaca gtctcgaaag aagaggtgtt cgtttccagt ggacattttg aaaatgcaca
     6541 aaaatttgct gctgaaactg gcgtccatgc atgcaaaagt aatcaggaag ttgcatcaca
     6601 atcagatatc gttcttttag ccgtgaaacc aaaaatcatt gaaaaagtat tggaagaact
     6661 aagagaatat agccagaaag tcttattcat ttctattgct tcaggaaaat cattaacaga
     6721 tttagaaaca gcgcttgaag atcctcaggc ctccatcgta cgtgtgatgc cgaatgtcaa
     6781 tgtcagcgta ggagcaggcg tttctgcgat ttgcaaaaat gaagctgcca cggaggaaca
     6841 gcttcagcaa gctacgatga tttttgaagc aatcggcagc gtctatcctt tagcagaatc
     6901 agatttcagt acattcattg gccttgctgg cagctcaccc gcattcgttt acatgctgat
     6961 cgatgcgatg ggacggactg gtgtactgaa tggtttgtcc aaacagacag ccactgagat
     7021 tgctgcaaaa gcaattatgg gttcttgtca aaaattcctt tcaagtgata aaaatccatg
     7081 ggaacttgta gaccaagttt cttctcctgg cggtacaact gttgcaggtg tagttgcctt
     7141 agaagaagca ggattcatcc cagcaatcat caaaggaatc aatacgacga tcgagaaaga
     7201 tcttgagatg ttgaataaaa aataatgata gcaatgtttt ttcgtgcctt acaaaaaaag
     7261 aagagctgtg gcttttgccg cggctcttct tttgtatttt ttattattta gcataatcag
     7321 ttgcacgagt ttcccgaata acgattactt tgatgtgtcc aggataatcc agttcttctt
     7381 cgattcgttt acgaatatca cgtactaaac gaacagcgtc taaatccgag atttcttctg
     7441 gcttgaccat tacccgaact tcgcgtccag cttgaactgc gtaacttgat tctacaccgg
     7501 cgaagctgtt cgaaatattt tccagacttc ttaaacgacg gatgtagttt tctaatgatt
     7561 cacttcgcgc accaggtctt gctgctgaga gcgcatctgc tgcagctaca aggacagaga
     7621 taacagaagt agcttccaca tctccatgat gagaagcgat cgcgttgatt actactgggt
     7681 tttctttgta cttggcagct aattccgcac cgatctcaac gtgagatcct tcaatctcat
     7741 ggtcaagtgc tttcccaata tcatggagca acccggcacg ttttgctaat tggatatctt
     7801 cgccaagctc tgctgctaag ataccagcta attttgcaac ctcgaccgaa tgcttcaaga
     7861 cgttttgtcc ataactagtt ctgaatcgca gacgtccgat gatcttgatt agatctggat
     7921 gaagtgtatg agcaccgact tcaaacgctg cctcttcccc gtattcgcga atacgttcat
     7981 ccatttcttt acgagatttc tcgaccattt cttcgatacg cgctggatgg attcgtccat
     8041 cttgtatcag tttttccaag gtcatccgtg cgatttctct tcggattgga tcgaatccgg
     8101 aaagaacaac tgcttccggt gtatcatcga tgattaaatc gataccagtc agcgtctcaa
     8161 gcgttcgaat attacgacct tctcgtccaa tgatacgacc tttcatttca tcattcggaa
     8221 gggtaacgac tgaaacagtc gtttctgaaa cttgatctgc tgcgcagcgt tgaatcgcta
     8281 atgataataa gtttttcgct ttgcgatctg cttcttcttt ggcacgctgt tcagattctt
     8341 ttaccattag agttaactca tgattcagct cttcttcagt cgatttcatg atgacatctc
     8401 gtgcgtcctc ttttgataaa ccagcaattt tttctagttc tttttgttga tcttcaatca
     8461 ttttttcaac gtctttttct cgctcttcaa ttaattgctg ttttgaacta agtgcttctt
     8521 ctttactttc aagtgagttc tcgcgttttt ctaacgagtc atctttacga ttcagcgttt
     8581 gttcgcgttg aatcaaacga ttctcttgag attttaattc taacttactt tctctcagct
     8641 cactttcaac ttcagaacgg tatttctgat tttcttcctt agcttctaac aatgcttctt
     8701 tttttagagt ttctgcttct ttttttgcac tatcgatgat tccagcagct gatgattgtg
     8761 caccagctat ttctttctcg tgacgtgact ttgcgaccat aaaccctaaa ccaagaccga
     8821 caattaaacc gatgatagcg aggagaatgt tgaataccat aaaatccacc tccatactat
     8881 ctttttcatt tttatcatct atacagcttc tatgataaat tattctaaac tcgtcaatat
     8941 gcatcttcgc atatatgtgt acaaaaaaca tgcacaactt tattctaatg tttgtacccc
     9001 gtagtgtcaa ctttaaataa acaaagaaac ggctttctac ttattcatta aacgatggtt
     9061 tttaaaataa atgacactag tttcatatag gtaaaccttt aaattgagaa acttttctat
     9121 taaattattc tttgtagatg aacagatatt tttattttat gtacccactc gtataattaa
     9181 ataactcacg atttctttat tcaataaaaa aacggcaggt caaggagagt acctactctt
     9241 cagaccagcc gtttttgttt atcataattt ttattcttct actaaaggca attcttcttg
     9301 tcctttttca tcttctggga cagcgacttc ttcaccaata ccataagctg ctctaactag
     9361 agcagaaact tctgccatca tttcaggatg attagccata tagattttag cgttttcccg
     9421 tccttgacca atccgatctt ctttataaga ataccaagca ccgcttttat caacaatgtc
     9481 tttttctact gccatgtcta gaagttctcc ttcttgtgag atccctaaac catacatgac
     9541 atctacttca gcgattttga aaggaggtgc tactttgttt tttacgactt tgatctttgt
     9601 acggttacca acaatatctg taccttgttt caattgttct gcacgacgta cttccaaacg
     9661 gatcgttgcg tagaatttca atgcgcgtcc tccaggagtg atttctggat tcccaaacat
     9721 cacgcctact ttttcacgga tttggttgat gaaaatagcg attgtttttg ttttgttgat
     9781 cgaaccagag agtttacgca atgcttgaga catcaaacgt gcttgtaacc cgacatgtga
     9841 gtcacccatt tcgccgtcga tttctgctcg tggaactaaa gcagcaactg aatcgactac
     9901 tacgatatct acagccccac ttgatactaa agcatcagcg atctctagac cttgttctcc
     9961 tgtgtcaggc tgtgaaagaa gtagttcatc gatattcaca cctaattttt gtgcatattg
    10021 cggatctaac gcatgctcag catcaatgaa agcggccgtt ccgccatttt tttgtacttc
    10081 tgcaatagcg tgtagtgcaa ctgttgtttt acctgaactt tcaggtccat atacttcgat
    10141 gatacgtcca cgggggtaac cgccgacacc taaagcgaca tctaacgcaa gagagccact
    10201 tggaattgta gagatttgtt ggtcgatttt ttcgcccaat ttcataatag agcctttacc
    10261 atagctcttt tcaatttttt tcagtgcagc atctaaggct gctttacgat catctgccaa
    10321 tacgataatc ctccttatat ttacaaatca gagcaacatt tcattatcct tatcatatct
    10381 tttttagtga aaaaaagcaa gaaaaaaccg aacaaatatt cgcatttatc attttcttaa
    10441 aagctgtttt ctcaaaagat caaatccttg cataaccgca ttatatctga tagcagcacg
    10501 atctctggca aagtgatatt tttccgaaac cacgccgtct ttagatgaaa tagcgatcca
    10561 tactgttcct gcagattgac cttctaacgt gtccggtcca gccactccgg tgaaagctac
    10621 actgtaatct gttcttgcta tctcacgtgc ttgaatggcc atctgctcca cacattcttt
    10681 gctgactgtt ccatattctg ctaatagctc cggatcaatc tccagaaaac cagcttttgt
    10741 ttggagcgag taggtaacga atccgcctgg aaatacttca gatacaccag caatgtcgcc
    10801 taatgcggct tggaaggcgc cggctgtcaa actttccgct gctgttacag tctgcttgtt
    10861 ttctttcagt agttccacta cgacttttgc tagcgaatta tcatcgccgt atccataaaa
    10921 atattcccct actcgttctt ggattttttc ttctaatgct aaaagagctt gattccctgc
    10981 ctgaacatca tttgttttca ctgtcagtcg taatgtcact tcatttggtt tggcatatgg
    11041 tgcgatcgtt ggattgatct gtgtctcaat cagatctttt agctcagtga ccagtcgcga
    11101 ttcaccaatc ccataaaagc gcaaaacttt agaaattagt ttttcttcag aaggaaaacg
    11161 ttcctctagt aaaggacgta cttgttccac aaacattggt ttcaactcat taggcggacc
    11221 gggaagaagg atatacgcat gtgtatctgt ttgataaaaa gtccctaaag caagccctgt
    11281 acgatttggt agaggaaccc cgccttcgat aatctgggac tgttgcagat tgtttttggt
    11341 catttttcga tgggttgttt caaaatatgc caataatttt ttgtatcctt ccgtattttg
    11401 tatgagagac ttaccgaggt gagccgcagt tgcttctttt gtcaggtcat cttctgtcgg
    11461 tcctaatccg ccacataaga gtatgagttc acttcttgtt tcagctagag caatcaactc
    11521 ttctaaacgc tgctgattat ctcctacaac tgtttgataa taaacttcga tacctagatc
    11581 agctagctgt tctgacaaga aagtggcatt cgtattcacg acttgtccta gtaaaagttc
    11641 tgttccgaca gctattattt ctgctttcat tacttcccgc tcccttatat agtatacgct
    11701 gtatttttat atttttttat ttaaaaagag ggcaaggagc tgtgacaaaa atcttgtcac
    11761 agctccttgt tccaaataaa cagtgttctc ctgccaaaac ctcggtcata agtcacaata
    11821 tacataaaac atgaaaagct gttttttgaa attatccctt gtgactcaga gttagacggc
    11881 aggctcacaa cctctccact aaacgatact tacattgatc ctttgaacac accgctgttt
    11941 ttgatgaagt aatccacacc tgaatagata gtaaagatca gacaagcata aagcatgatt
    12001 tgatctactg gaataccgat caggctgaat gggatattat tgataaacag caagataata
    12061 gcaaccattt gtgtagccgt cttgactttt cccggccaag ctgcagccat gacttctcca
    12121 tgttcgacaa gtagtaaacg caagcctgtg acggctaatt cacgacagac aatgatcgct
    12181 actacccaac caggggcctt tccttggtca accaacatga taaaagcagt cataactagc
    12241 attttatctg ccaacgggtc cgcaaacttg ccaaaattag tgacaaggcc tcgcgcacgg
    12301 gcaatttttc catctaacca gtctgtgata ctagctaaag caaaaataac tgcacctact
    12361 agctgtgtga cagccagtgt ggtgtctcct acgttaaatt gtccccaatc taaaggtaaa
    12421 cctacaacca aaatgaaaat cgggatcata caaattctaa gtacagttaa tttatttggt
    12481 aaattcacac tatcgactcc tttcttccct attctacgtg actatcagcg aagtttccac
    12541 cgatattatt tacttttcca aatgaattta ttcaaaggag tatttttatt gaaattgatc
    12601 tttttgaatt aacgaagaag gaccgcgaca cagatttgtc acgatccttc ttttttatac
    12661 ctacaagtaa acagatgcgt aaactaattt atacgatcat tctgcgtatt gaagagtaag
    12721 gttcacattt tttggactct gattgttttc ccctggatta actggtacgg attctccatt
    12781 cgctttgatc gttgcattgc ttgctgcacc aatcgtgatg gtcgcatttg ttgctgtttc
    12841 cggtaacacg gtactctgtg tttcatttgc tgccagtgtc ccttgataaa cataggcatt
    12901 gtctactagc acacctaacc atacacgatt gacagcagta aattcaaggg ttacaggctt
    12961 ggtagcgtct gtgatattta ctgcgatggc actgccagaa tcttctcctg tagctattgc
    13021 cattttcttt tcttcacttg attttgttgt tgattctact gtagaagact ctttggtctg
    13081 cgtgctttgc tctgaggaag aggactcctc gactgatcct tctactttaa gcgaagaagc
    13141 tgtgtctccg attattggat cagcttgacg atcttgccaa gtcatatagc ccacgacaac
    13201 gacaatcgcc aaagcaacca atcctaataa aatgacagga agacttgtcc aaaagcgact
    13261 tggatgcgat tcttcctgat gcattgcttt tctagaagta cttactgttt caggctgcgg
    13321 acgttcaggg accgatcctg tcaatttgct tttcccatca aaaacatcga ctaaatggtc
    13381 tccatcttct cctacagctt cagcgtattg ccgaataaat gcccgcacgt aaaatttacc
    13441 aggcaattgg tcaaaatcat cattgtcgat tgcttccaaa taacgttttt gaatttttgt
    13501 aatctgctgt aattcatcta aagatatttt tttattcagg cgtgcttgcc gtaatttttc
    13561 tccgatggtt tcaaaagcca ctcgttcatc ttctccagtc tgtttgattc tccgaagcat
    13621 ctgctgttgc aacgaaagac gttatgtttg ggtgctgtga caaaagtcat gtcacagctc
    13681 cttttttccg aataaacatt gttctcctgc caactcctcg gtcacaagtc acaatatcca
    13741 ataaatataa gaagctgttt tttgaacttg ttccttgtga ctcggagtta aacggcaggc
    13801 tcacgacctc tttcttaaac ggtgttcaat cagctgttcc tcggtattag ctcaaaaacc
    13861 ggcaaaatat aaggagatat ttttccgtat gtttctctaa tactcaggtc aaaacgctga
    13921 tttcacaacc tcttccttaa acggtgttca aaagcttgca tctgcggtaa taagtccaac
    13981 caagttaaaa acattaagaa gctgttttta cttggctgtt cttattactt gatgcagggc
    14041 agctttttca caacctcttg gtacacggtg ttccagaagt agcttcttcg gcaataagcc
    14101 gaaatatcac aaaaatttga gaagcaattt tcgtaaattc cttcttattt cttgaagctg
    14161 accacttctg tcacaacctc tttgattact tcgtttgatt ttattttaat ataagttgat
    14221 taaaagagag catgatttgt tttattttct cactaatttt tccaacacaa ctaagaatac
    14281 cataaaagct gattttggag gaggttccta ctacaagtct aaaacgaaat ttaagacttt
    14341 tcctcataaa tactggttta ttttggtctc atgtaaaact cgctgaaggc ctcttcactt
    14401 ataaatgctt ccccagctgc cagtacgtct ttcatttgga tcgaatcgat gatttcagga
    14461 agatcaaata aagttcgatc gccaaataaa ctttgcgtaa attgattggc aatatattcg
    14521 atcgaattta atgattggaa atattgtcca agcattttct tcttcagcaa atcgagattt
    14581 gtttctgaca cttccggatc atcagcgaat ccaagaatga tttctttgac tttttctgct
    14641 gctttctctg gttcgtccgt atctccgctg aagtccgcaa agtgaaattc acgatccaag
    14701 ctgaattcaa aaccaaagct gtcatcgata attccttgat tgtacatcgc tagatagttt
    14761 cgagaagtat tgcctaaaat catttggaac aacagattga ttgctgtttt atagcgtaac
    14821 agttctgttc cttcttgcgg caatgtgtct aatcctttaa taccaagtac aaatttatct
    14881 cgtgtaatgg ctgcttccaa tgcactttgt ttgatgattt cttttgtgtt ttcagggaaa
    14941 taacgtacaa tttcttgctt tggagggaaa ttttttgctt cttggttttc ccgaataagt
    15001 ttcatcaatt tctcaggctc catcttacca actacaaata aaaccatatt gcttggctga
    15061 taaaacgtcc gatagcacgt atacaagtct tgagctgtga ttttgtcgat actttcaacc
    15121 gtgcccgcaa tatcgatatg caatgggtgt gtaggataca agttatttag tatgccaaaa
    15181 aacatccgcc agttagggtc gtcttcatac atttggattt cctgaccgat gattcctttt
    15241 tccttgttta cagtttcttc tgtgaagtaa ggtgcttgta caaaatcgat cagagtcgtc
    15301 agattctttt ctacctgatc cgtcgtagaa aacagatagc tggttttcgt aaaacttgtg
    15361 aaagcattgg cggaagctcc ttgtttccca aataattgaa agacatcacc atcttctttt
    15421 tcaaacaact tatgttctaa aaaatgggca attccatctg gaactttcac cttttctttt
    15481 tcaccatagg gaataaattc gttatcgatc gaaccataat tcgtactgaa caaaccatat
    15541 gtcttgtgat agtcattttt aggcaacaaa tacacggtca aaccgtttgg cagtacttca
    15601 tgataaagtg tttcattgat ttgctcatat tctgttttat tcatttgcat ttcctcctgc
    15661 taaaaagaag attgcctgca attcgatttg ctcagctact tgttgtatct ctttgatcgt
    15721 tacgctctcc atacgtttca accattcttc atcagataat tttgtttccg gcagccagct
    15781 gtccaaaaaa gaagcttcga ttgccgcttg cggagagtct agagaaagca gatattggtt
    15841 tctcagcatt gcttttgtct gagacagttc caactctgtt atttcaccat ttcgcaaact
    15901 ttctaattgc tcgtgaatca gatgaagtac ttgatttcgg ttcttttcat cgataccggt
    15961 ttggacactc atgaatcctc ggaacgtatc cacgctacta gaggcataat aagctagact
    16021 ttctttctct ctgacattca tgaaaagttt agagtgagga aaaccgccaa ataaaccgtt
    16081 aaagaccatt agcgcaaaac gttctggttc atcgtaatac acatttgtct gataagctag
    16141 gttcagcttt gcttgaacga ttggctcacg tacttgtcgt tccttcacga tattcacttg
    16201 aggttgggta taaaacagat ctggataaac tgtttcccgc gactcaaatg gtaattgccc
    16261 aactgcttca gctgctttgt ccggatcaac atcgccgaca acaaaaatgt cgatttgatc
    16321 atttgccatc atttcttgat agtaggacgc tagcgaacta gcagtgatct tgtccaaatc
    16381 ttcgactacg cccaaccctg ggatcttttg ttccgggctg ttttcaaaat aaagttcttg
    16441 caaggcatag gaagcaaaag tttgtttatc ttccttcaaa ctttccaaat atagacgcag
    16501 attgttcttt tccaagtcaa acgtcaactg atcaaactga tgatttttaa tatttggata
    16561 aaataaaacc tcttttaaaa aatctaccgc ttgagaaaaa aggtttggat cattgacata
    16621 ttcaccattc acaaaactga tccctgcgtt tagccaatgt aaatttcctt tttttctaac
    16681 tgataatcca aagcttgcgc cataaagctc tgcaagttgc gcacttaact ttgtctgatc
    16741 cggataattc aaactatttg tttccaacat gctgctgaga agtgaacgtt tcgtgatgac
    16801 ttcttgttgc agacgcgcag taaaacggat aaaaatccgg atcgttttgt attgttttgt
    16861 tggtaagaca tgaagattca cgccttctgc caattgatac accatattta tatgctcctt
    16921 ttcacagttg attgcccgct tactctttta atgggtcaac ctgccttttt tacttattcc
    16981 tctgcttgaa atttataagt tcaggcaatt cgccgttttt tctaaatgat gttacacttt
    17041 atagtatagc atagatgttt tttatcaaaa gaaacagacg gctaaaagcg tatattgttt
    17101 ttttattttt tatttttcga tatactaaag acaaataaaa gaagggaggg tcattcatga
    17161 tcaaagaatt caaagagttc atcatgcgtg gagatgtctt ggatcttgct gtcggcgttg
    17221 ttattggtag tgcgtttaca agcatcgtaa accaagtggt gcaaggcttg atcacaccac
    17281 taataggttg gtttgtcgca atgatcacag gtacttctga tttagaagga gcactttcaa
    17341 ttcttgactg gtcgccgaca aaaggcgtca catttgcgtt tggtaacgtt attagtgcta
    17401 tcattacatt tctgatcact ggttttgtct tattcttagt tgtcaaagcg gcaaaccgtg
    17461 cgaagaattt acgctccaaa gaagagcaag tggaagtgga agaaactcct actgcagaag
    17521 attatcttag tgacatacgc gatcttcttg ccaaacaagt ggcagaagac aatacacatc
    17581 cagtagagca aacaaatctt tccgatactt ctaaataata aagaatctaa aaaaaagaaa
    17641 gccgattttc agtgtcggct ttcttttttt agattcttaa cttgctactt gttccttttt
    17701 catgtcgata tgtggaatat catcttccaa atacacatct gagattgcct tgaatccaaa
    17761 cgactcataa aaagcttgta ggtattcttg tgcgccgatc acgattggtt tttctggaaa
    17821 atttttctgg atcatgttaa tcgtttcttg aacaatcttt cttcctaaac catctttacg
    17881 gttgtcctga tgaacaagga cacgtccaaa atggatactc tccgcttctt catatactcg
    17941 cgtgtaggca aggatcttcc catcagtatc ttgaaaaaac gtatgccaag cttgtccatc
    18001 aatgtcatca acttcttgat aaggacactg ttgttccaca acaaaaactg cgattcgttc
    18061 tttgacgatc gcgtaaaact ctgctgttga caattcttca aatttcttta cctgatattc
    18121 cactaagcaa acgccccctg cttttttatt cttagcatac cgattcgcac acaaaaaagc
    18181 aaacgaacct tacgaatc
//
LOCUS       DAYYGO010000056        17951 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000056 DAYYGO010000000
VERSION     DAYYGO010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.057"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(24..722)
                     /locus_tag="ACHA5T_001591"
     CDS             complement(24..722)
                     /locus_tag="ACHA5T_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFM8887169.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(715..2067)
                     /locus_tag="ACHA5T_001592"
     CDS             complement(715..2067)
                     /locus_tag="ACHA5T_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFM8887170.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2290..3690)
                     /locus_tag="ACHA5T_001593"
     CDS             complement(2290..3690)
                     /locus_tag="ACHA5T_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFM8887171.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3851..4588
                     /locus_tag="ACHA5T_001594"
     CDS             3851..4588
                     /locus_tag="ACHA5T_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFM8887172.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4713..5192
                     /locus_tag="ACHA5T_001595"
     CDS             4713..5192
                     /locus_tag="ACHA5T_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFM8887173.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5189..5956
                     /locus_tag="ACHA5T_001596"
     CDS             5189..5956
                     /locus_tag="ACHA5T_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFM8887174.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6186..6755
                     /gene="lepB"
                     /locus_tag="ACHA5T_001597"
     CDS             6186..6755
                     /gene="lepB"
                     /locus_tag="ACHA5T_001597"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM8887175.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6859..7461)
                     /locus_tag="ACHA5T_001598"
     CDS             complement(6859..7461)
                     /locus_tag="ACHA5T_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFM8887176.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7567..7797
                     /locus_tag="ACHA5T_001599"
     CDS             7567..7797
                     /locus_tag="ACHA5T_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887177.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            7788..8036
                     /locus_tag="ACHA5T_001600"
     CDS             7788..8036
                     /locus_tag="ACHA5T_001600"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8887178.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            8142..8726
                     /locus_tag="ACHA5T_001601"
     CDS             8142..8726
                     /locus_tag="ACHA5T_001601"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFM8887179.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8729..9802
                     /gene="prfA"
                     /locus_tag="ACHA5T_001602"
     CDS             8729..9802
                     /gene="prfA"
                     /locus_tag="ACHA5T_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFM8887180.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9795..10634
                     /gene="prmC"
                     /locus_tag="ACHA5T_001603"
     CDS             9795..10634
                     /gene="prmC"
                     /locus_tag="ACHA5T_001603"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFM8887181.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10627..10806
                     /locus_tag="ACHA5T_001604"
     CDS             10627..10806
                     /locus_tag="ACHA5T_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887182.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10828..11853
                     /locus_tag="ACHA5T_001605"
     CDS             10828..11853
                     /locus_tag="ACHA5T_001605"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFM8887183.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12044..13288
                     /gene="glyA"
                     /locus_tag="ACHA5T_001606"
     CDS             12044..13288
                     /gene="glyA"
                     /locus_tag="ACHA5T_001606"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFM8887184.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13441..14388
                     /locus_tag="ACHA5T_001607"
     CDS             13441..14388
                     /locus_tag="ACHA5T_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFM8887185.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14522..15151
                     /gene="upp"
                     /locus_tag="ACHA5T_001608"
     CDS             14522..15151
                     /gene="upp"
                     /locus_tag="ACHA5T_001608"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFM8887186.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15290..15532)
                     /locus_tag="ACHA5T_001609"
     CDS             complement(15290..15532)
                     /locus_tag="ACHA5T_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887187.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15653..15725
                     /locus_tag="ACHA5T_001610"
     tRNA            15653..15725
                     /locus_tag="ACHA5T_001610"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15686..15688,aa:Thr,seq:cgt)
     gene            complement(15844..16482)
                     /locus_tag="ACHA5T_001611"
     CDS             complement(15844..16482)
                     /locus_tag="ACHA5T_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFM8887188.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16605..17237)
                     /locus_tag="ACHA5T_001612"
     CDS             complement(16605..17237)
                     /locus_tag="ACHA5T_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFM8887189.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            17588..17887
                     /locus_tag="ACHA5T_001613"
     CDS             17588..17887
                     /locus_tag="ACHA5T_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM8887190.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT         6153 a         3147 c         3552 g         5099 t
ORIGIN      
        1 tcacattccc ctttttcgaa taactaggct gtttgtcctt caagataggg cagatccaat
       61 tctttaccgt aacgttgttc taaagctagg cggatcaaac gagctgccag gttctcattt
      121 cttgcaagga taggtccatg gaaataagaa ccgaagacat ttcggtaaat gacaccttca
      181 gattggtctt caccattatt tccttgacct tgtaccactt tccccaaagg tcgttcacct
      241 tcacctaaga aagtccgacc attgtggttt tcaaatccat aataagtttc attgaattct
      301 tcgttatgga taacgatatc accgataaac cggttatttt cctgacttaa cgtgtagtga
      361 tccagtgcac tgattccttg gatcttttct ccattagctc ccatgtagta gtgacctagc
      421 aattggtagc caccacaaat ggctaacatg acgccatcat tttcgatata gttgatcaaa
      481 gaatctttct ttttctggat gtcttttgaa atgattaatt gctcaaagtc ttgaccaccg
      541 ccaaagaaaa ccaagtcata tttatcagca tcaaaaggtt catgtatact aacgatctcc
      601 gtatggcaag aaacccccat tttttctgct atatatttca acatcaaaag gtttccgtta
      661 tcaccatatg tgttcatcaa atttccataa aggtgggcga tattgagttc ataattagcc
      721 acggttcatt cctcctttga taaatccttg agcagctagt tctttacgaa gctgcaatac
      781 agcagtgtaa gtagctaaaa tatagacatg ctcagttggc aattctttga ttttttcgat
      841 tacttctggt aattcttttg tttctgtcaa tttattttct ggaatcccgg caacttttag
      901 acgtaaagtc atatctgtat gacggtctcc tccagaaata acagctggga tatccatatc
      961 ggcaaagttt tcaaaattac catcccaaat ccagctgaca tcgattccgt ctgcgtaatt
     1021 ggcatttaat aaagccacta aagaaaatgg ataaggagct agaccaatca tatcgattac
     1081 ttgattgata cctacaggat ttttgacaag tactaatgta cattttttcc cattgatatt
     1141 gatgacttct tgtcggccga atactttctc gtcatatcct aaaccttgtt tgattttatc
     1201 aggatcgaca ttgaagtact ctgccactgc agtagctgct aaagcattgt agacattata
     1261 cataccgcca acttcgattc cgtattcatg accatcgatc acgaaatctg ctgacacatt
     1321 gtccatccgt accatatcag ttaatcgata atccaattca ggacgtttga agtcacaatt
     1381 tggacagtaa tatttcccta agtttgcata agtgatcatt ttataatgca agatgtgatg
     1441 acaatttgga cataagacac cgtctgtatt gtaatgagcc atttgttcac gatcttcttc
     1501 atggtcaaag ccataatatt ttctaggatt gactgtttcg atagaattga agattggtga
     1561 atcaccgtta ctgataatcg ttgcatttgg tgactttgcg gcaccgtcta caatcatttt
     1621 ataggtcgta tagatttctc cataccgatc catttgatca cggaaaatat ttgtaaataa
     1681 gaacagttct ggtttcatat attctgtgac tttgctcaaa cttgcttcat ctatttctag
     1741 gacagcaaac tttttctgtc ctttttttgg ttttgcctgc aagaaagtcg atacaattcc
     1801 ctgtaccata ttcgcaccag tagggttggt tagaacttca tcgaattgct ggcgcaaaat
     1861 attgactgtt aaagcggtag tcaatgtttt accgtttgtt ccggtgacaa cgacgacttg
     1921 gtagtctttt gccaaactat ctagtatatg tggatcgact ttcagcgcta gtttacctgg
     1981 taaacttgat ccgcctttaa aaaaagtttt taatgcccat tgcgacgttt ttccaactgc
     2041 aatcgcaaaa tgactgcgta ttcccattat gtaacctcca aaaacgattt gctgacgaat
     2101 aaattcaact aatcatacca tatgttgaaa aaaaggttaa attgcttttc ttattcttaa
     2161 gaaaaggtta cggctgaaag tgcatgaaat cggacaaaat cgcatgattt aacaaaaaaa
     2221 cagcttgaaa caaaatgatt gtttcaagct gctgtgattt tattattccc tcttacctat
     2281 attcccattt taaccgataa taattttctc gcttggatat tcatagccta gatctcgtgt
     2341 ttcttttggc acgaataaca tcaatgtata tagcattccg atccgcccaa taaacatcag
     2401 tgtagcaatc gtgattttgc ccacaacaga caagtctccg gtaatcccta aagataaacc
     2461 agttgttcca aacgcagaag ttacttcgat gatgatcgag atcagcgttt gttcttctgt
     2521 agcagataaa aaaacgatac aaaagaaaca cataccaaga gaaagcataa agacaacgac
     2581 tgattttcgc acatcatctt gatcgattct ccgtccaaaa atattgatgt tatcttcact
     2641 tttgaggaag gaatatagat acaaaccgat gattgctaca gtagttgttc gaatcccgcc
     2701 gcctacagaa ctaggactgc atccgataaa catcagtaaa gaaaaaatga tcagtgttgt
     2761 gatttgaaaa tcacccaaat cgtgaatctg cagaccagca ttacgagtcg taatcgaata
     2821 aaacatcgaa ttgatccatt tgacactcat attggaatct tggaataaat ggtccttttc
     2881 caatagataa atcaagacag ttcccgaaac aaacaagata acaaaagcta acacagctaa
     2941 tttggtaaac aaggaaaaac gaaacggaag cttcgcattg gaacgtttgt ataatagcca
     3001 ttcccggcac tccatcaata caggaaaacc aattcctccg acaaaaatca aaaacatgat
     3061 caggatcaag aaaaaatagt catgtgcaaa gggcttgatg gaatccccag tgacgtcaaa
     3121 cccagagttc gtgacagccg atatcgcctg ataaaaaccg taaaaaacgg catcacgcca
     3181 acgatcaaaa tagcctctgt aataaaaata aatagaaaag aatgtcccaa aaagtaattg
     3241 aaaccagatt aaaatagcaa aagtgatccg aatcagtcgg acgataccag acaaacgcgg
     3301 ttgattcata tccgtcatga tcagctgtcg ctgtttcagt gtgatccgtc gtttagaaaa
     3361 aatgacaaaa gcagtagaaa tcatcatgat ccccaaacca ccaacttgaa ataatatttc
     3421 cagcagtata ataccgttat cattgaacac agaattgata tcaaaagtcg atagtccagt
     3481 cacactcact gtactgattg ccatgaacag catgtccaca aaagaaacat ggctatctgg
     3541 ttcgcgaaaa atcggcatat aaaatagaac gagtgataag accgtcatca atatataata
     3601 aaaaacgatg atttggatcg atgagaaatg attggcagca aacttctgtc ctcgccgctg
     3661 cagccttcga aagaataaat caacatgcac agtactatcc tcctgacaat agtcgtttct
     3721 accagtatac aaggggaatt aggaatactc aaagaaatta tcaaacagat aacaatacag
     3781 ctttcgatta aaatagaaat ggcttataat aaggaaaatc ctagaaaaaa gaaggaggag
     3841 gaataacaaa ttgattaatg ctcaacaaat cgtagaaaaa atgaaaaatc aaaaaataaa
     3901 ttatgataca gttctgaaaa aaatgatcat gcaatgggag aaagaagaag taaagcctaa
     3961 gatattaata catacatgct gtgctccttg cagtacgcac acattggaat tcatgtctca
     4021 atatgcagag atcacgttat ttttttcaaa ttcgaatatt catcctaaaa gtgagtattt
     4081 gcgaagatta catgaacaaa aacgatttgt agaccaattt aatgagaaaa ctgaccatca
     4141 tgttgcgcta atcgttgatg aataccgtcc aaatgaattc accaaaatgg ttttaacaaa
     4201 ccatcttgaa aacgaaaaag aaggtggtgc tcgctgcagt gcatgcttca atatgcgatt
     4261 agatcttgtt gcgcaaaaag cgcaagaact aggatacgat tactttggaa gtgccctgac
     4321 catctcgcca aagaaaaatg cagctttgat caatcagatc ggaatggata tccaaaagat
     4381 ttacgggaca caatacttgc caagtgattt caagaaaaac aaaggctatg aacgctcttt
     4441 gcaaatgtgc aaagagtatg atatttatcg ccaatgttat tgtggttgcg tgttcgctgc
     4501 caagcagcaa ggatgcgatc tgaaagaaat caatcgggaa gcaaaagctt acttaaaaac
     4561 atcaaccgta gtttttgagg aaaattaaag aaatctttcg gaaaaacgta agaaaaaaat
     4621 aaagaattta tggtagaaat aaggtagact gtataatttg tccgtagtaa agtaaggaaa
     4681 caagaataaa tttcaatgaa ggaagagata agatgcgaaa agtagcacct attttgaagc
     4741 caatcgctgt attaacaatc tcgacgattg tcgcgactac tacaggtatt gtgaatagtt
     4801 ccagctaccg tgcaccaagt tcaagttcta gtactgtaga agaaacaaca ccaactactt
     4861 cagtctcgac atctgaacca acaacaacgg aaagtttgaa tgaaaccact acgacaagta
     4921 catgggacga tacaacaact agttcgtcgt atgatgagac atatacaact gacagctcaa
     4981 gcgaaacaag taatacggta ccagatggca ctccatcgga ttcaaccaat ccaagtacaa
     5041 gtccggatac aagtgctagc tccgaaacga taccaagcag tacgactgta gatgataaca
     5101 cgcaaaatga tgatgcagca cgtacagcac agcaaaacca agattcagga aatacccaaa
     5161 cgtataacca gcaagcaggt gattctcaat gacattttta ctagacctta tcaacggggt
     5221 gcataaattc cttggctatt tagacatcag tcctaaatat cttaaccgtg gatatacgat
     5281 tttaagtgtg attcccacgc tttatttatt gagaattgtt tatggacttt ggcaaaacca
     5341 aaactatctt caatttttct tatatggact tgcatttcta gtgcttgttt attttactgt
     5401 tttaaatgta ttttattact ttttagataa aaacacgaaa gccgatgtca ctcagctttt
     5461 tgtgaagtat ttacctgatg aagcgttcaa catccagaca gaagaatcga tagataatgg
     5521 aaattcgatc gataaagtga atacacaaga ggtcatggtt tcttatgacg aagattatca
     5581 attgaaatta gccgagaata tgcggtattt gattggaaat ggtgagatca aaacaaatga
     5641 actcggtcgc atggatggat ttctggtcga tagaaataca ttgtatcctt attactatgt
     5701 caagaaaaca ggagagaaag agtatgttct aaatattgga cgaagctact cggacatgga
     5761 aaaagtaggg accattcgac ttgactcaac agaagacaca ttggaacctg tcggattgtt
     5821 catcgtcggc ggtgattttg tcaaagacgg gttacgctat catgagcctt atcgtctgaa
     5881 attgattgca aaaaaggcac agtcgcagga agaaccagtg gtttatagtc gtagccaaag
     5941 aagaagacat tcataatgtg atgaaacgcc gtgtatcagt ggttgcgata aaaacgttta
     6001 ttcggaataa gggggatgtg acacgtcttt tgtcacgttc cttttttata taacgagaaa
     6061 gataagacga acacatcact tattctttcc tttcatttat aaacattgta gaataaagaa
     6121 agtacaacga agtattgtga aaaaacgtaa taaataacga ggaaaataca agtaggaggt
     6181 aagctgtgaa agaatttttg aaaaattggg ggattttgat ccttgtatta gcggctattt
     6241 tattagctag agtgtacgtg tttacaccag ttactgtaaa cgggcattcg atggacccta
     6301 cattaagtga tggacaacga ttgatttcat ctaagatctc aaactatgaa cgaatggata
     6361 ttattactac aaaggaaccg ggggatgaag aacggatgat tgttaaacgg atcatcggta
     6421 tgcctggaga tacagtcaag atggaaaatg accagttgac cattaatggc aaaaagtatg
     6481 atgaacctta tctggatgag ttcaaaaaag aattttcaga agataagtta caaggagaat
     6541 atgcttatag ctcaggattc caagcacaag cagaaagttc atccaccttt acgagtgact
     6601 ttgaatatac tgtgccaaaa ggaaagtatc ttgttctagg cgataaccgc ttgatttcaa
     6661 aagacagccg aatgtttggg ttagtcgaca aagatatgat ccaaggaaaa gttgttttcc
     6721 gttattggcc tctttcagaa attaaaatca tgtgatgaaa aaataaaaag aagctgtgac
     6781 atgttttttg tcacagcttc tttttccgaa aattccttct tatttcttgg agttgctcac
     6841 tttttttgca atctcatttt aagctttttg gctgatggat aagaaaagcg aagcgttttt
     6901 gttcataaat agcttttccc cacgatggaa ggtatgacta gtagcagaga caaagcccgt
     6961 tttttccaat cgattattca attcttcttg gctaaagcca ttatggactt tcggatgact
     7021 aatttgttca ttcttgtcaa agtctacgat gatcaattga ccgccaggag ctaaaatttc
     7081 ataaagtttt gtaagaatat tctccgtatc aggaatgtgt aaaagtacaa gtgatagaag
     7141 gatgagatta gcttggacag caggaaattc gacagaagca tcagcgtgga tcgttcggac
     7201 atttttcaga tctgctgctt ggatcttttc ttctgccatt ttcagcatag tttctgaagc
     7261 atcggcaatg atcagttctt tgaatctttc cgcaagcggt aaactaacaa gcccagttcc
     7321 gcctccgtaa tccagcagga ctttttggtg gctgtcgcgc ggtagataag tagttagttc
     7381 ttcgcgaata atcttggcta atgcttgacg gtcaggggaa tcataatggt tagcgatgga
     7441 atcaaaaata ttttttgtca tcaaatagct ccttacgtaa aactttgaaa ttttaactta
     7501 ttatagcacg tgaaaagaaa gaaagcatgc tatactcaat tcaaaaaggg agaatctaga
     7561 aagaatatgc cgatatcgtt tgtaaaagat agagaggaaa aaggaaagtg tgtccgagaa
     7621 atattactag acttgcctga gtggtttggg cttcctgaat caacggaaaa atatatcgag
     7681 gaatcaagta aactaccttt atggtgtgaa aaaagaaaag aagaatattt aggctttatc
     7741 actctttcac agacaagtga agatactgcc gaaatctatt ctattgtatg ggaataaaaa
     7801 aagactatca tcaccaagga ttaggaacaa aactttttaa agaagcagaa ggttacgcag
     7861 caaaacatta caaatatttg caagtgaaga ctgtagacga ggggcactac tctatatatg
     7921 atcaaactat ttgtttctac gaatcattag gattttcaag gttggaagtt tttccaaatt
     7981 tatgggatga atggaaccca tgtttagttc ttgtcaaaaa attggaacaa aagtgagcaa
     8041 tagatttaat actaatctgt gcttgatgaa aattctcact gtaaaaaatg ctgaaaatca
     8101 agtataatac cataggaaca aactggaagg tgaaaaagag catggcacaa ctatttttta
     8161 agtatggcgc aatgaatagc ggcaagacga tcgagatcct aaaagtcgca cataattatg
     8221 aagaacaaaa taagccagtt gtcttaatga caagcggtat tgatacaaga gatggtttag
     8281 gcgttgtttc aagccgaatc ggactgaaac gtaatgcgat gccgatattt gaagaaacag
     8341 acgtatttga cgtaattcag aaaatggaaa gcaaacccta ttgtgtgctg atcgatgaag
     8401 cgcaattctt aaaaaagaaa catgtcattg cttttacgag aattgtggac gaattggata
     8461 ttccggttat ggcatttggt ttgaaaaatg attttcgaaa cgagttattt gaaggatcaa
     8521 aatatttatt gctttatgca gacaaaatcg aagaaatgaa aacaatctgt tggttctgcc
     8581 acaaaaaagc aatcatgaat ttacattata ttgatggaaa ccctgtttat gaaggcgatc
     8641 aagtgcaaat tggcggaaat gaagcttatt accccgtttg tcgaaaacat tactttcacc
     8701 catcaatgat tacagaagga gaataagcat gtacgatcaa ttacaagcaa ttgaagaccg
     8761 ttatgaagag cttggagaac ttttaagtga cccagaagtg atttctgata caaagcgttt
     8821 tatgcagctt tcaaaagaag aagcaaacac gcgcgaaaca gtcgaagttt atcgtcgtta
     8881 taaagaagta gtacaaggaa tcaccgatac agaagaactg cttggtgaaa aactggatga
     8941 tgaaatggct gaactagcaa aagaagaatt atctgaatta aaaaaagaaa aagaagtctt
     9001 agaagaaaaa atcaagatct tgcttttacc aaaagatcca aatgacgata aaaatatcat
     9061 catggaaatc cgtggagcag caggcggaga cgaagcagca ttattcgctg gcgatctttt
     9121 caatatgtac caaaagtatg ctgaagcaca aggctggaaa acagaagtga tggaagctaa
     9181 cgttaccgga atcggtggat ataaagaagt gatcatgatg atcaccggag acaacgtata
     9241 ttcgaaatta aaatatgaaa gtggtgcgca ccgcgtccag cgtgttcctt caactgaatc
     9301 gcaagggcgt atccatactt ccaccgcaac agttgtcgtg atgccagaag ctgaagaagt
     9361 agagatcgat attgaagaca aagatatccg tacagatatc tatcatgctt caggtgctgg
     9421 cggacagcac gtcaataaaa cagcatctgc tgtacgtttg actcacttac caacagggat
     9481 cgttgttgct atgcaagatg aacgttctca aatcaagaac cgtgaaaaag caatgaaaat
     9541 cttgcgtgct cgtgtgtacg ataagatcca gcaagaagca caaagcgagt atgatgccaa
     9601 tcgaaaatca gcagtcggaa ctggggatcg ttcagaacgg atccggacgt ataatttccc
     9661 gcaaaatcgt gtgactgatc atcgtatcgg attaactatc caaaaattag atcagatttt
     9721 agcagggaaa ttagatgaga tcgttgatgc attgatcatg tatgatcaaa catcaaaact
     9781 ggaagctatg caaaatgggt aaaatgacct atcgagaagt cctgtcacgg gcttcttctt
     9841 ttttagagga tcatgggaaa gaagcatata gtatccaatt tttatttttg gaacgaaaac
     9901 aatggaaaaa acttgattgg ctccttcata tgaacgaaga gatttcagaa gaagaacagc
     9961 ggttgatcga aaccgatctg cgacttctgc ttgcagatca tccacctcag tatttgctag
    10021 gatatgcaga tttttatgat catcggttaa aggtaactga agcaacactg atccctcgac
    10081 cagaaacaga ggagttagtc gaatggtgtt tagacgagac gcctggtgta ccgttagaag
    10141 tgatagatat tgggacaggg actggagcaa ttgctattag tttgaaagca gcaagaaaaa
    10201 attggcatat atctgctgtg gatctttcag aagaagcatt agaagtagcg aaagaaaatg
    10261 ctcagaaaga aggaacgaaa atcagttttt atcatggaga tacattagga ccagtaatgg
    10321 atcagcaatt tgatgtgatt atttctaatc cgccgtacat cagtcggaat gaatgggaac
    10381 tgatggatga aagtgtccgg tcattcgaac ctaaaatggc cctttttgct gagaacgatg
    10441 gattagctat ttatgaaaaa atagcagttg aagcaccaag cgtactaaaa tcaaatggaa
    10501 agattttttt agaaatcgga tttcgccaag gtgaagcagt aaaaaaaata tttcagcaag
    10561 catttccaga taaaaaagtg gcgataaaaa aggatctttt tggcaatgaa cgaatgatcc
    10621 aagtttatgg ttgaagttgt ggatgaaaaa ttttttattt gtttttttat tataaaaaaa
    10681 cacttatttg acaaggatgt aaacgacttt ttggtttatc aacaagttta tccactattg
    10741 acaaatagat tatccacaaa cagtggaaaa cttgaggata aactcagaca aaataagaat
    10801 gtatgaaaaa ggggttgtgg ataaactgtg gaaacgaaac gatatgatga aacagaattg
    10861 caagaggcag ccgaagcact gaaagaagga gaacttgttg cttttcctac cgaaacagta
    10921 tacggattgg gcgccaacgc tcttttacca aacgctgtca agaaagtgtt ttctgtaaaa
    10981 gggagaccgc aagataatcc attaatcgtc catgtcgcct cttttgaaca agtaaaagag
    11041 tatgtggaca actttcatcc ggcagcagag aagattgtga agaacttctg gcctggacca
    11101 ttaactttga tatttaagat caaaaaagac actttgccct cagttgtgac gggaggatta
    11161 tccacagctg catttcgtat accggataac aaaaaaacac tagaagtaat cgaactttcc
    11221 ggtgttccgc ttgtcggtcc aagtgcaaat acgtcgggaa aaccaagtcc aacgactgca
    11281 gatcatgtat atcatgatct acaaggaaaa attaccggga ttattgatga tggagctacg
    11341 cgaattggtg tagaatccac tgttcttgat ttaagtgatc caacagccat gcctatgatt
    11401 ttacgaccag gtgcagtaac gaaagaacaa atcgaagcag tcatcgaatc tcctgttgct
    11461 attgatcagc atcttgtaaa agaaaatgaa acaccaaaag cccctgggat gaaatacaaa
    11521 cactactcac ccgatacacg tgttctgatg gtcagagatg gcgattggtc aactgcagtt
    11581 caatgggcaa aaaataagaa gatccgtgca ggtgtcatag caagccccga aattgcggat
    11641 caagtgcgga cagatactgc agctgtttat atgtataacg acaactcagt agaagcggcg
    11701 gctaaaggat tgtttgcagg tctaagagga ttagatgaac caacacttgg attagatttg
    11761 atttttgtac aagtgtatcc agaaactgga ttggggaatg cttacatgaa tcgcttgaaa
    11821 aaagcagcag gacaaaatta ttttgaaaaa tgatcaattg gttattgtcg cattctttta
    11881 gtatgataca atgctatcaa gtaaatcttc tatcagtgaa cgaaaaacta agcgtaacta
    11941 atcgtaatag aagagatacg aatatttggc aatttgtttt tgatgggaaa aataaaattg
    12001 taggaaaata aaattgtagg aaaataaaaa ggagctgttg tttgtggtag attacaaaac
    12061 gtttgaccct gatctttggg ctgcgatcgc caaagaagaa gaaagacaag aacataattt
    12121 ggagctgatt gcctctgaaa actttgtctc cgaagcagtg atggctgcac aaggaagtat
    12181 cttgacaaat aaatacgcag aaggttatcc agggcatcgc tattatggtg ggtgtgaatt
    12241 tgtcgatatc gttgaaaatt tagcgattga ccgtgcaaaa gaattattcg gagcgaaatt
    12301 tgcgaacgtc cagcctcact ccggctctca agccaacacg gctgcttatc tcgctttggt
    12361 agaaccaggg gacacgatct taggaatgga tctttccgca ggcggacatc taacacatgg
    12421 atcaccagtc aacttcagtg gcaaaaccta tcactttgtt gcttacggtg ttgatccgac
    12481 aacagaagtt attgattata atgtggtacg tattttagct agaaaacatc aaccaaaatt
    12541 gatcgtagca ggtgccagtg cttacggacg aacgatcgac tttgcgaaat tcagagaaat
    12601 cgcagatgaa gtaggcgcaa aactgatggt agatatggca catatcgcag gattagtagc
    12661 agcaggactt catcctaatc cagttcctta tgcagatatc acaacgacta ccactcataa
    12721 aacattgcgt ggccctcgag gcggcatgat tttgacaaac gatgaagcat tagcaaaaaa
    12781 aataaatagt gcggtatttc ctggtattca aggcggccct ctagagcatg tgattgcggg
    12841 taaagcagta gcattcaaag aagcattaga tcctgcattt aaagaatata gtgaacaaat
    12901 catcgccaat gcaaaagcaa tggtcaaagt tttcaatcaa gcaattggaa ccagagtcat
    12961 ttcaggggcg acagataatc atctgatgtt gatcgatgtt cgcgagttag gtataaatgg
    13021 aaaagaagca gagagcattt tggacagtgt gaatatcact gtaaataaaa actcgattcc
    13081 ttttgaaaca ctaagtccat tcaaaacaag cggtattcga atcggcacgc cagcaatcac
    13141 tactcgcggg ttcaaagaag aagatgctgt aaaagtggct gaactagttg tcaaagcatt
    13201 acaagcaaaa gatgataacg ctcaactaga cgaagtcaaa acaggtgtac gcgaattaac
    13261 tgaaaaattt ccgttgcata aaaaataaaa atttaaattc cattagtaaa cagaaaagtt
    13321 caatgttggg cttttctgtt tattttttgg gaaaacggat tctttgacaa tatcggtgat
    13381 gctctctagt atatgaggtg taattttagt cttcacacta aatctcaggg aggaaaagtc
    13441 atgactgatc gtatctcgtt agaaaaggaa gcagtagcat tcagtgatgc aaatgcgcct
    13501 catccgcgta tctatgaact agcaccggaa gaaggaagag ttttactaga agaagtacaa
    13561 gaatcacctg tgaacaaata ttccgtcgat attgaagact tgatggtgga tactggcgaa
    13621 tggggaaaac tcaatattcg tctcgtacga ccagaaggga atatggataa attaccagtt
    13681 attttttata ttcatggagc aggctgggtt tttggcagtg ctcaaaccca tgataaattg
    13741 atcagagaac ttgcggttcg tacgaattcc gtcgttgtct ttccagaata tagtcgatca
    13801 ccagaagcaa aatatccaac agcaattgaa caaaattatg ctgtgttgca acagctgaat
    13861 aaattagcca agtcaaaaaa tctggatctc gagaatctaa tcgttgcagg agattcagta
    13921 ggtggtaaca tggctacagt catgacgatc ttaacaaaag aacgaaaggg cctcccaata
    13981 tccaagcaat tgctgtatta tccagtcaca aacgctgatt ttgatacaga ttcctatcat
    14041 gaattcggag aaaactattt tttgacaaga gaaggaatga tgtggttttg ggaccaatat
    14101 acgacgaatg aagaagagcg tgctgaaatc acagcttcgc ctttgagagc aaatataaat
    14161 gaactaaatg atcttccttc agcaatgatt ttaacgggag aagcggatgt attaagagat
    14221 gaaggtgagg cttatgcgcg caaacttcgt gatgcagggg ttcctgttac acaagtacgc
    14281 ttccaaggaa tcatccatga ttttgtcatg gtaaatgcta tggatcagac aaaggcaacg
    14341 cgtgcagcaa tggcattatc tacttcttgg atcaagaaaa aagaataagc tttgcttgtc
    14401 cgcaggaaaa cttcttttca aaaacggatt ttcttaggaa aaatggcgag tttgactagt
    14461 caatcacttg attttgtttt acaattaaaa agaaacgaca gaacaaagga gagcttaaaa
    14521 aatgggcaaa tttcaagtga tcgatcatcc attgattcaa cacaaactaa ctattattcg
    14581 ggacaaaaac tgcggaacaa aggtttttcg tgaagtagtc gatgaaatcg ctatgttgat
    14641 ggcatatgaa gtatcacggg atatgccatt ggaagacatc gtgatcgaaa caccgatcac
    14701 tgaaacaaca caaaaaacgt tgtctggtaa aaaagtagcg attattccaa tcttgcgcgc
    14761 aggtattgga atggttgacg gtatcttgga attgattcca gctgctaaag tgggacatat
    14821 cggtctttat cgagatgaag aaacattgga accacatgaa tatttcgtca aacttccaga
    14881 agacattgat gcacgtcagt tgtttgtagt agatccaatg ttggcaacag gcggatcagc
    14941 aatcatggca atcgatgcat tgaaagaacg tggcgcgtcg aacattaaat ttgtttgttt
    15001 ggtagcagca cctgagggag taaaagcttt gcaagaagcg catcctgata ttgacatcta
    15061 tacagcttct ctagatgaaa aattgaatga acatggttat atcgttcctg gactagggga
    15121 tgcgggtgac cgtttgtttg gtacaaaata agtaaatacg aaaaagggtg tgacacaact
    15181 tttgtcacat ccttttttgg tcatttttaa cgtttgatgc agaacagctt tttcacagct
    15241 ttcgtttgca tataaaaaaa ccatcccaaa aatgcagaga tggtcaaatt caaatctttt
    15301 ttagttcttt gtagttaacg attactacac ccttactttg cgtcatcaag ttcgcatttt
    15361 ctgcagtttc atcaatctca acaacagcgg aatttttcaa ttgctttgaa atcgttccag
    15421 tttgcgactt gccgtgaatt gtgaacatta cgtgagtatt gatcttgaat ttttcaccaa
    15481 tttccgatgg tacgatagtt gtatcaaatt ttccaaaagt tgccattttc ataaaacctg
    15541 cctttcacat caattatacc acgttttatg aaaaatatca tgaaattaaa ctaattttcc
    15601 atcggactct ttacaatcag ccaaatagag tatataattt cttttggata aggccagctt
    15661 agctcagttg gtagagcaac gcactcgtaa cgcgtaggtc gtaggttcga tccctatagc
    15721 tggcattaaa agagaagaca gatatatcaa ggatttttaa tccattgata catctgtctt
    15781 tttgtttaaa ggaaataaag tatgctttct tagcaataga gcatgtatta tttctataaa
    15841 aatttaggag aataaaacga ctagcaatat agctgtgaaa tcaaatatca catgaactgc
    15901 ccaacttgta atgatcgagt tgcttttaat ggcagcttgg ttgaataaca atcttgccac
    15961 cccttgtatc aaaaggatgt gtgctacagt gtgtaaaata tttccattat cataagtact
    16021 gtaatggata aatccaaaga tgattgctga caagaaacta gcgacaggaa tgctgaactt
    16081 actagaaaaa atagcgagaa aatacactga gaacaattct tctccaagta tcatgatagg
    16141 caaaataata aataataacg ggctgaaagc ctcatttatt ggattgcctg ttaaacccca
    16201 tttcaaaatt tgttgcaaaa ataccgagac tgcggcactg attactacat tgataaaaaa
    16261 atatttggct atatttttga caggagcgac aggtttcgag aacatttttt tcgtatcttt
    16321 tttaccaaaa acaagtgtaa atgctataaa tccagtgata gttaaagaaa aaatgctaat
    16381 aatggatggc gcaaatagca accctaacaa accgagtaca acgacaatag tgcccaatat
    16441 ccctttttta agttctattt tttttcctaa aatgttattc atgataataa ctcctactat
    16501 tcagtttttg aagatagagt cagtatacat gaagagttaa aataggcaat gtttttttat
    16561 ggatatttgt attgctaaaa atttatctta tatattcatt ttgattatac tacttttatt
    16621 tgtaaaaatt ttctagcaaa accaagtgct tgcaactgat taagcgtaca attttttaga
    16681 tgttccgagc gtacttcaat catttcaaag gagttgctac ttaaatcgat attttttaaa
    16741 tcagtatcaa aaaagctagc attttctaaa tcattagtag aaaacattat tttagtgagg
    16801 gaacagtcta caaaatctgc gcttgataag acagagtgct caaaggtact ttcttttatg
    16861 tttgaaccag ataaatttac atagtccatt ttacaattct cgaataacgt gttcccaata
    16921 taactttcca tcaatttagt acctgttaat ttacaattga caaatttcac gcgatgcaac
    16981 atgctttttg tcaatataca gcctgaaaaa tcacagtttt cgaatataca gtcggtaatc
    17041 tccattcttt gaaaatcatt ggaagcaaaa aatgcatact tgaaaataga tttgtaaata
    17101 attggatggc agattatttg gttttcgatg acctcttttt gtgaaaggat attatacagt
    17161 tcaaaatctt cgttttcata gatatgaaaa aaggatcctt tattcaagct tgcttcatta
    17221 actttaggaa aaatcatatt taattacctc cattttcata aagtcatatt aacaaaagct
    17281 aaaccaactt acattagcag aaaatgagat aggttttcgc ctttttcgat aacgatagtg
    17341 tcgctatctc ttgaaacgtt cttagaattg ccctgagaga cacaaattag gcgtaattct
    17401 aataaaaatc ttagaataaa aatttttact gatttaattt agaatgtttt taaaataaaa
    17461 atcgttgcca taacagtgta ttgaaaggtt ttaacaatcc agtaatgatt taaaaaaatc
    17521 tagatgtagc gtatactttg tttatagaat gtagtgggaa gaaatatatt tttgggagga
    17581 aatcgtgatg tctaaaagtg aagaaataga aaacatggta ctaaaattat atgatctcgt
    17641 tttaaatcca gagatcaaag aaaaggaacg tatacttttg atagacgcaa agacaggctt
    17701 agaaaaaggt cagtattatc ctaaagtcat aaataatttg gaacgtagtt tacgtccgtt
    17761 agcaatcagg ggagaattgt ctaaaccagt atcaccgttt tatatggaga tatcaacaat
    17821 aggaaaattt gaaaaagaat taggtcgagg aatggcttcg gcaccgatta cttttgggca
    17881 tttataaaaa cgagttattc atctcaatga attaaaaagt atagtcaatc tcataatgca
    17941 ggctaggttc a
//
LOCUS       DAYYGO010000057        17929 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000057 DAYYGO010000000
VERSION     DAYYGO010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.058"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(143..457)
                     /locus_tag="ACHA5T_001614"
     CDS             complement(143..457)
                     /locus_tag="ACHA5T_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887191.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
     gene            743..982
                     /locus_tag="ACHA5T_001615"
     CDS             743..982
                     /locus_tag="ACHA5T_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887192.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(1344..1604)
                     /locus_tag="ACHA5T_001616"
     CDS             complement(1344..1604)
                     /locus_tag="ACHA5T_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887193.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVIGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1789..2286)
                     /locus_tag="ACHA5T_001617"
     CDS             complement(1789..2286)
                     /locus_tag="ACHA5T_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFM8887194.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(2416..3126)
                     /gene="budA"
                     /locus_tag="ACHA5T_001618"
     CDS             complement(2416..3126)
                     /gene="budA"
                     /locus_tag="ACHA5T_001618"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFM8887195.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(3139..4803)
                     /gene="alsS"
                     /locus_tag="ACHA5T_001619"
     CDS             complement(3139..4803)
                     /gene="alsS"
                     /locus_tag="ACHA5T_001619"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFM8887196.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(5008..5670)
                     /locus_tag="ACHA5T_001620"
     CDS             complement(5008..5670)
                     /locus_tag="ACHA5T_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM8887197.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(5680..6495)
                     /locus_tag="ACHA5T_001621"
     CDS             complement(5680..6495)
                     /locus_tag="ACHA5T_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8887198.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(6757..7200)
                     /locus_tag="ACHA5T_001622"
     CDS             complement(6757..7200)
                     /locus_tag="ACHA5T_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HFM8887199.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(7334..7672)
                     /locus_tag="ACHA5T_001623"
     CDS             complement(7334..7672)
                     /locus_tag="ACHA5T_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8887200.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(7660..8037)
                     /locus_tag="ACHA5T_001624"
     CDS             complement(7660..8037)
                     /locus_tag="ACHA5T_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8887201.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            8261..9454
                     /locus_tag="ACHA5T_001625"
     CDS             8261..9454
                     /locus_tag="ACHA5T_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8887202.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(9620..10042)
                     /locus_tag="ACHA5T_001626"
     CDS             complement(9620..10042)
                     /locus_tag="ACHA5T_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8887203.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            10413..11087
                     /locus_tag="ACHA5T_001627"
     CDS             10413..11087
                     /locus_tag="ACHA5T_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887204.1"
                     /translation="MKKLVALFLVAGSLLSGCGATTSTTQSTSSAQASSSSTTISEST
                     ADSSKEQEQIEKKKEEEKQKAEKAALEKKVTEAESALSQAEANPTKENYATASAAIQA
                     LPENKQELTSRLAAIDSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQ
                     AAAEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPC
                     SKCYGN"
     gene            complement(11249..12421)
                     /locus_tag="ACHA5T_001628"
     CDS             complement(11249..12421)
                     /locus_tag="ACHA5T_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8887205.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(12627..13925)
                     /locus_tag="ACHA5T_001629"
     CDS             complement(12627..13925)
                     /locus_tag="ACHA5T_001629"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM8887206.1"
                     /translation="MHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYP
                     SKDTAVPADATPFASVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLS
                     LPFYNPNSSDPDKPLDTIHLYAKNVIKESNIKIQKAGNFSGVDKLAGAQFQIKNSENQ
                     FVTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVETKAPDGYQLTNPQ
                     KETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTITVENVQNYGDFHF
                     IKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWSDEVTGSDWEEVKL
                     ISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDDDPTTSDETTIDNQ
                     PKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(14053..14568)
                     /locus_tag="ACHA5T_001630"
     CDS             complement(14053..14568)
                     /locus_tag="ACHA5T_001630"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFM8887207.1"
                     /translation="MDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFT
                     SSKDGTFSVHGLSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEP
                     KANPTNNGKTPVQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKK
                     QQKGNNRREIK"
     gene            complement(14641..15555)
                     /locus_tag="ACHA5T_001631"
                     /pseudo
     CDS             complement(14641..15555)
                     /locus_tag="ACHA5T_001631"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
     gene            complement(15552..17879)
                     /locus_tag="ACHA5T_001632"
     CDS             complement(15552..17879)
                     /locus_tag="ACHA5T_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HFM8887208.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
BASE COUNT         5228 a         3539 c         3094 g         6068 t
ORIGIN      
        1 taaactctag agaaattaat tcatcctaac ataaaataat tgttcactca aaagtttttc
       61 tagaacaatt aaaacagcag gcttcacatt cgctatacat ttgtaagaag taataattcc
      121 tgtactaaaa ggcataattg ctttaattat tttttgactt ctcttcaaac acaagaagta
      181 gttgtgtaat aattgcagaa ataaggattc caataataga tataaaattt gacatggaaa
      241 cgtctataac attaaaagaa taaagtgttg aagaaagtaa actgattgaa ataacccctc
      301 cgtaaaaaat agtctgtgaa tatacccact ttttttcaga agacgtactt cgtttggttc
      361 taaaacctat ccctgcattt ctactcttat caaaaaacca aatatttaga agtaataata
      421 ttgctattat taagataaaa ttactgtaaa aatccaaaag ctatcctcct agcaactatt
      481 taaataccca aaaaaattac ctctagataa tatcatacct acgttttcac ttataaattt
      541 tttcataaaa aaagcgttta gaggcataat cgttaataca gatggtaacc aatgtatctg
      601 gtttgaactg ggatatcacc ctctccatat atgtattact aagactaata gatactataa
      661 aagacttgat agttttgcat tgattttgcc taatactgag cataggtgta tgctaaacga
      721 tactataagg agtgtgtaca taatgaaaag aattctgttt atcagtttat caaatttatt
      781 gttacttact gcttgtggga cttctgcatc aaagaacata ccttcagata cgcaaatttc
      841 gaatagtatc gattcaacaa tttctacaaa tgagaaaaat tctccggtag aaacttccga
      901 tataaaagtt tctacctctg caaaaaacag ggtaaagaaa ctgtcagcac gtctccttta
      961 tttcaaacaa actctgaatt agcttcttca tcttcttcag caaataatga tgatagtttc
     1021 gcgtttactc ctaactaaat gcaaccttta caaatgcttt acgataacaa gcctgagcta
     1081 aatgatcctg atatcagttt tgcatttttt gaaatgattg gatcggatga tctttttaaa
     1141 actactagtt tgtctatccg aaaataggac agttctggca gtataggttt ttatcgagtt
     1201 tctccaccag gcctcgttac agagacagat tctctaggcg aaccttttga ataaatcaaa
     1261 aatcatgaac aatcactgcc aactaaataa agtgccttca aaagcttggt cttctcgctt
     1321 ttgaaggcac tttatttaaa tctttatgaa actaaagtat gttcatattt gttgctatag
     1381 ttatatagat attctgttgt ttccaactca taaacgtgag tatattttgc cactactttt
     1441 ccgcattttt ctaatatttc attgcgatcg acgtcatgat attcgtctac gcagatcaca
     1501 acacaatttt ctaatttact gattacttct ccaatcactt tttgtttcaa gccaataggt
     1561 tggcaaatat atttttttcc taaaatgata gtttcgttat tcatatttca tccatcctta
     1621 atgttttttc attatttaaa atgataataa cattaaaata tgattatgaa aatcaataac
     1681 acttgtgatt ttataaaaaa ataaaaaaac cgaaaaacag aaatgcccac tgtctttccg
     1741 gctcttttta ttcttgattt ctcttctttt ttgtttcaat agacgatttt agttattaat
     1801 aaatgaaaaa gcatttatat taaaatttgc taataaaaga tctacatatt tttctagaaa
     1861 tgcttgatcg atttcatcat accctttcgt tactccgcaa tcgatatcta aaacaccgat
     1921 taaattacct tcttttacca ttggcactac gatttctgat tgtgcggctg aatcgcagga
     1981 aatgtaattt gcatgggttt tgacatcatc aacgatcaat gtttcttgtt tttcagctga
     2041 ttctccacaa actccttttc ccatcttgat acgtgtgcaa gaaactcgcc cttggaacgg
     2101 tcctaaaatc aattcattac catcgaataa ataatatcct gcaaagacag tctcaggcaa
     2161 agtctcattc aacaatgcag atgaatttgc taaatttgct aaccagttat cttctgcttc
     2221 taataatcct tgttgctgta ataataaaat ttcgtatgca tcttttttct gtttttctgt
     2281 ccacatgata aaaaccgctc ctttttgatc tatggagaag attatagctc agtttttttg
     2341 taaacgctag gttttctcct caaaaaaacg agatacgtca ggttaatcac cttgaacgca
     2401 tctcgctgtc tttttctatt ctgcttgttg gatttctgct gctaaatcag cataatcaat
     2461 cgtttcgtga acaaaagtcg gatcatcaac tggaaaatgc tgtacaaggg acgagatgcg
     2521 gctaatctca acttttcctt cagccacttc aaaatccatt acatgtccgc caaatgtatg
     2581 tgtatcatcg ataaaatgca ggtgaaatcc cgctgcagca actccttgga ataattcagg
     2641 cgtaaagaac ccgactaatg ttccttggac attgctttgt tggaattctg gttgagactc
     2701 tgaaattttt gctagtcttc tgtaaggttt ttcttgcttc ggcatgatac ggatgtgcat
     2761 atttttgaat aatccagtta tcttcactgc caaaaatagg ttttccccgg cttctcttac
     2821 taatatgtct tttttcaatt gttctccatc tgcagttttt gagacagaaa aactagtatc
     2881 tggcgtaaaa tctgtaattg ctgcataggg ggtaagttca cttccatcta attgggcaac
     2941 tgagccatct gatcttcctt gataagctac gccatctaaa aagatgactt caccatcaag
     3001 cccatgcaat gttcccaatc ccagagttcc tttttctaat atatgagcaa tcgtttctgt
     3061 tccatccatc aagccagcca tcaaagctcc taatgtcccg tgctggtata agattttttc
     3121 agtcatgtcg acacttcctt aatagaattg atctggtaag attgtttctc ccaatttctc
     3181 attatcacgg taatcaatcg gaatatcaat aattacgggt ccttctgtcg caaatccttc
     3241 ttctaaagct ttttctaatt cctctacact tgtagctcgg attcctttag cgccaaaagc
     3301 ttctgcatat ttcacaaaat caactggacc aaagtctaca cctgacgccc gctgatattt
     3361 cattttttct tggaactcta ccatattgta gtgtccatca ttccaaatca aatgaacgat
     3421 attcaatttt ttacgtaccg ccgtttctaa atcctgggca gagaataaaa atcccccatc
     3481 gccggaaact gatacgattt gtgtatttgg acgcaccaat gcagcagaaa tcgcccatgg
     3541 caatgcaaca ccaagcgttt gcataccatt actgaataac aaatgacgag gttcataact
     3601 tctaaagtgg cgtgccatcc aaatataatg actacctaca tctacggtaa cagtcatatc
     3661 atctgttact tttgactgaa gcgtgttaat aacttctaaa ggatgcaaga tacctgcttc
     3721 tccttttgat tgaacaatgt cccgctccgt taattctgct tgaagagatg ctaaatattc
     3781 ttttgcatct tctgacactt gttgaggctc taatgaacct gtcaataaat caagagtggc
     3841 tgagatatca ccgatcaatt ctctttcagg ctgcataaaa ggatcgattt cagctggcgc
     3901 ttcatcgatc acaatgatcc gcgcatcttt ttccgcattc cagtttcttg cttcatattc
     3961 gattggatca tagccaatag caataactag atcacttcgt ttcaaaagca tatctcctgg
     4021 ttggtttcta aataaaccca cacgaccaaa gaaatgcgct tctaattcac gtgagatcac
     4081 tccagctgct tggaatgtct ctacaacagg caatgctgtc tttccaacta attgcctgat
     4141 ggctaacgtt tctttttcac ttgatcctcg catacctact aatagtaccg gtagttttgc
     4201 ttctctaatg cgttcagcta ggtaacggat atcatctgca gaagcagaac ctaatttagg
     4261 atcgctcaat ggcttgatga cattcccttg gacaggtgca tccactacat cttgcgggat
     4321 actgataaag ctcgcgcctt tttttgaact cttagccata cgataagcat tggcaatcac
     4381 ttctgaaatt gtttctggat cttggatttc tgcactgtat tttgtgattg gctggaacag
     4441 tgctgcatta tccatgcttt gatgggtcaa cttcaataga tcgcttcgtt ttacttgtcc
     4501 ggcaattgct aaaactgggt caccttctgc tgtcgctgtt accaaacctg tagctaagtt
     4561 actagcacca ggaccacttg ttgcgataac tactcctggc tctcctgtaa tacgtccgat
     4621 tgcttgtgcc ataaatgcag cattttgttc gtgacgagtc acgattaatt caggaccttg
     4681 atcctctaat tcattaaata caccatcgat ttttgcacct ggtataccga atacataatc
     4741 aacatgatga ttaatcaaac tttctactac agatttcgaa ccttgtttca ttttttcgtc
     4801 cattgaacat cgtccttttt atgctttatt tttaattatt agttagaaat aatcacaaat
     4861 aattgataca gtttctaaac ctgtttcatt caaaagtata gcacgtttta tttcatttgt
     4921 ttagccatat gtttcaaaaa atgaaaaaga atgacagttt ttacataaaa actgtcattc
     4981 ttaattgaaa gtttcaattt ataaagtcta cagatccata gttctcatca atttacgatt
     5041 gttgaagtaa atcaaaccaa atacgataga aggacctagt agaatacacc attggggctt
     5101 tacgaaaaag aagagaaaca aacttgtcaa tagtaaaaaa tctagtaaac tcacatacca
     5161 ttttttcaac gtatagtaga acgctatccg tagaacttga tttggtttcg acgctggatt
     5221 tctcacctga aaataccagt agttgatgct aaaggaaact gataaacaag caagtaaaac
     5281 aaacaacgga atcaaccatt ttccataaga aaaacgtata aaaaagaaac aatcaaaaat
     5341 cattatcaca ctaactatcc aagcaaatag ccaataaaaa aatcccttta gccaaaattg
     5401 gcgaaacgtc tttatgaaca aacgtactgg tgaaaaactt ttttcttttg ctaattcttc
     5461 tgtcatacct atcatcacga tgcaacctgg accaataaaa agtaaagaaa aaataaagga
     5521 aaagacattc cttccatcta tcgacaaaag gaacgcagct gctatataca agatattgac
     5581 acaccaaaaa caaaggctac ttgccaatga ttgatacaac caataaaaga attccatata
     5641 aacatttttg tcaaaacgct gctctttcat aatcacctcc tattccttca ttcctgatga
     5701 agcgatccct tctacaaaat atttttgcat cgctaaaaat ataaaagcaa caggaagaac
     5761 agaaacaact gaggcagcca tgattaatgc gtactctgca tcaaatagcc caataaacat
     5821 tttcaaccct aactggattg ttttattttc cgtagaagac aaataaatga atggccccat
     5881 ataatcgttc catgtattaa cgaatgtgat gatcgtcaaa ctggcaatcg ccggttttgt
     5941 aagaggtaaa atgatcttgc gatagattcc ccactctgat aagccatcga tccgcgcact
     6001 ctcacataaa gaatctggga tactactgta gtattgcttc aacaagaaca caccaaaagc
     6061 attgaacgct tgtaatagca ccaacgacca caacgtatat gttaaaccca cttttctcat
     6121 cataatgaac tgcgggatca tataagactg ccaagggaca gcaatcgtgc cgatatatgc
     6181 taaaaataaa atatcgcgcc ctttaaaatt tagtttagaa aacccgtaag cagcaaaact
     6241 agaagtaaac aactgaatca acgtgatcac aacgcttaaa aaagctgtat tcttaaaaaa
     6301 tgtcactaaa ggaatacgag accaaataac cagataattc tctaaatgcc aagttttagg
     6361 gatccattga atcggaatcg tgaacacttc attatttgct ttgaatgaag cagaaaccat
     6421 ccataaaaaa ggaatgatcg tcaataaagc taatacaatc agcagaatca tacccaacca
     6481 ttgaataaag ggcatgtttt ttgaacgaat ttttgctttt tttctttcat ttcgaacacc
     6541 tccaaagtta taatcttcat ttctttgtaa tgattacaat ccagaacaac ggttattata
     6601 gctgattcat ttccgttctt cttatgtttg taagcgctta tttaacaagt tatgtaaatc
     6661 tctttatttt ttttgattta aaaatgaaaa aatacaaaag caagactgtc atacgtctcg
     6721 cttttgtatt gaaatttctt cgattagaga tacttattac tgttcaaaag cttctttgaa
     6781 acggtgaatc aattcattga ctacaggtga taaaatccga ttctttttcc agactagcat
     6841 gcagttcgtt tcaatcattg ggtcaagcgg aacgaatgtc actcgctttg aaagatgact
     6901 tacagcacct tctatgacta aagcagcacc tacctgattc tcaattagcg caaatacatt
     6961 gaaaatcaaa ttgtacgttc ctgtgatcag caactgctcc ttttttatgc ctaaccagtc
     7021 agcaaacatt cgctgtattt ctggacgctt tgagatcaaa agaggtacat tcgcaaagtc
     7081 ttcttttgtt atcacgtctt tttttgctaa aacagaattt tctaaaatga gcaatcccca
     7141 tttttccttt cttggtaaca aaatcgtttc gtatttctct gtatctactg gatctagcaa
     7201 aatcgctaaa tctaacaggc ttttttctag tcactccgtg atatcatcac ttgttccgct
     7261 gtggatatga aattgaacgc tgggataatc acggatcaac ttttctagta atagtgccaa
     7321 tgtattcgaa ttatcagctt ccacacagcc gatcgtaaat cggccgctga ataattgttt
     7381 ttttctgttc tcgaattctt gagtggtttg attcgttaaa tctaaaattt cttctgctcg
     7441 gcttttttag aaaataacct gcttctgtca agatcatttt gcggttttcg cgtacaaaca
     7501 gttctgttcc tagttcctct tctaactcct tcagttgtcg gcttaaggtt ggttgtgtaa
     7561 gatgtagaac ttctgcggct tgagagattg ttccttcttc tgcaattgtc caaaaatatt
     7621 ttagtaagcg taactccata gcgatgctcc ttgatttttt tacttgatac attccatttt
     7681 atttctagat aacacacgat catatttctt gatttttcct tgtaaacgtt ccaaacattc
     7741 ttgcatttca gcaatttttt tcgtcaaaag ttcactttct tcaatcaaaa gcttacgccg
     7801 ctcaaataca gttgcttctc cttcgctaga taggcgaata tattcaatca aagactcgat
     7861 cgataatccg gcatttcgca tacagatcgt aaagtcgacc cattttaggt cttcttctgt
     7921 ataatcacgg attccgcctt ttgtccgatt aactggtgga atcaacccga ttcgttcata
     7981 atagcgcaat gtatcggccg aaatgccctt ttcttcactg acttgtttga tattcatctg
     8041 atcactcttt ctcttaattt cttgccactt tacttatgta ttttatttat tctacaactt
     8101 taagtgtact ccaagtcaat actcatttct attaaaacta agctggatta tctatatcgg
     8161 ttgtttgtgt attatttcaa ataaaaaagt cttgtttttt tattcttttt tggatatact
     8221 tttaacaatt tagggaaatt ttaggaaaag gggtataaaa atgacaacgt atcagcgaaa
     8281 gttattatta tcgacatcgg cgggaattgc tttagagaat atggatatta tgtttttagc
     8341 tttttcatta tcttcaatga ttacttcttt tcatattagt ggtacgcaag cgggtttcat
     8401 ttcaacgatc actaatttgg ggatgctcat tggaggtatt ttttttggca tcatagctga
     8461 taaatatgga cgtgtgaaag tattttctca aacggtcctt ctgttttcga ttgcttcttt
     8521 attgatgtat tttgcttcaa acatctactt ggtttatctc tttcgcttta tcgcaggtgt
     8581 aggagcggga ggagaatacg gggcgtgtat gtccttgatt tccgaaagtt tttcaaaaaa
     8641 acaaattgga cgagcttctt cggttgctgg tattggtgcg caggtcggtg cggctttggc
     8701 tgcgatttta gctgctgtga tcattccaac gtttggttgg aagatgttgt atgtcgtagg
     8761 tgtactgccg gtgttgatgg tcttgttcat tcgtcgcggc ttgactgaac caaaggaatt
     8821 caaatctgta caatctgccg aaaagaaaac caaacttagc agactgtttg aatcaaaaca
     8881 tcttgcttgg caaacatgtg gccttagtat catggtaact gtccaaattg ccggctattt
     8941 tgggttaatg aattggctgc cttcaattat gcaaaatcga ttagggcttt cggtttctgg
     9001 gtcttcattg tggatggtca gtactatttt aggcatgtct ttggggatga tgacttttgg
     9061 cataatcatg gataaattgg ggcctaaact agccttttct ttgtttttaa tttgttcatc
     9121 cttatctgtc tatctacttg tgttagccca tagccagtgg agtttggtag cagccgctgt
     9181 gatagtcggc tactttatca atggcatgta tggtggatat ggcgcagtga ttagtagttt
     9241 gtatccaaca gaaatccgcg cgacggcgaa taatttcatt atgaaccttg gacgagctgt
     9301 tggtggtttt tcttctatcg tcatcggttt cttgatggat cattatagtt taactgccgt
     9361 aatcttgttt ttgagcggta tttacctagt cagtctgctg gtcttgacaa ctcttactgg
     9421 tgttacagca ttaagggaaa gcttgcagca ttgatagttg agtctgtaaa tgacaagaaa
     9481 gaggtgtggc aaaagcttgc atctgcggta ataagaccag ccaaatcaaa aacatgagac
     9541 actgtttccg tttggctggt cgtattactt gatgcagggc agctttttca actgctaatt
     9601 tccttctatc tggcggctac tattctttga cgtccgtcat ttcaaagtac tttactaata
     9661 acttagcata acgaaaaact gtggatacac tcgtatattg agcgtctgca taatcttcca
     9721 aatttttctc aggatgttga tacatgaatt caatcaaatg ataattatat gattttctgt
     9781 agaaatgaag ataaataatg tagattggga agttctcctg cttttccata gaaaagcgcc
     9841 gattattctc attcttcaaa atgaccttat caggatattt ttggaacaat tcttctaaat
     9901 ctgttagata attcaagata gacttacttg ataccttcaa ttcgttttgt agttcttcta
     9961 ttgaacacca acgttctgtt tcaagcagta aatgaatcat tttaattttc ttttgagaaa
    10021 cttttgttaa aaaagtctgc atattttctc ctcctgtatc tactttacta tactacatac
    10081 aagaaaaaaa acggatacaa atatcatatt gtcacaccgt caatagtgca taaacctgtt
    10141 attatttctc tttttttcct aagtattcta ttgatatgaa tcatttaatc gtttattata
    10201 taaatgtcaa taatctaata ataaaataac gtacatattt tattattgca tacttaataa
    10261 cagaactata gcaaaaaatc ccgtgtatca aaacgggttt agattaagtt tcctcaatca
    10321 ctattcataa cacaattatt ataacaagta agtaaacaat tgaaaaggac atttattgaa
    10381 aacggttgtt ttttacttaa ggaggaaatt tagtgaaaaa attagttgca ttgtttttgg
    10441 tcgcaggaag tttgttgagt gggtgtggtg ctactacgtc tactactcaa agcacaagtt
    10501 cagctcaggc ttccagcagc agtaccacga tctccgaaag tacggcagat tcatcgaaag
    10561 aacaagaaca aattgaaaag aaaaaagaag aagagaaaca aaaagcggaa aaagctgcac
    10621 tagaaaaaaa agtgacagaa gcggaatctg ctttgtcaca agctgaagca aatcctacaa
    10681 aagaaaacta tgcaactgct tcagcagcta tccaagcgct acctgaaaac aaacaagagt
    10741 tgacaagccg tcttgctgca attgattcca caatcaaagc aaaagaagca gaagaagaac
    10801 ggaaaaagca agaagaaatt gcacgacaac aagcagaaca agcgcaacaa gaagcaatcg
    10861 cacagcaaca agcagaacag gcgcgacagc aagcagcagc tgaacaggcc tctcagcagg
    10921 cacaagccgc agctcctgag caaaataatg aacaaactgt ctatgtcaca ccgaccggtt
    10981 caaaatacca tacacataaa tgtggaaatg gtacgtatac ccctgccact ttaagcgaag
    11041 ctcaagctcg tggtttaacc ccttgctcca aatgttatgg aaactaattc aaaagttata
    11101 ctataataat aaaattcttc tgaaacataa aaaaatagaa gctggcgttc cagatagtaa
    11161 accaaaaata gcagtttaca cgtccatttc gtcagcttct ctcttttttc atttagtctg
    11221 ttccctctgc ttttttggac atcttcattc attctttctc aatcaaatag tacacctctt
    11281 tttcttttcg cagtttatat cttccttttt tcaatttcat tgaaaactgt ttcgaatgtt
    11341 tatgttttac atggatttta agcaggagct cctcatgttg caagcgatag ttcctgcctt
    11401 ttaaatcaga aaatctcact agtccgtatt catcagaagc tttcacaaat ggaacttttt
    11461 ctctagtaat atagtgtttg ccgttgagcg tttttacaga aaagaccatt ccttcgattg
    11521 gtcgtccgtt tgtgttcttt acataaaaag ataaaggata gtttcgctta gcgatggcca
    11581 aagaatctaa ataagcgatg agtagccaaa ctaagccgat tgatccaagt agtataccga
    11641 taaaccaaat aaaaaatttc cattttttcc attgatcaat tccttggatc gctttttttg
    11701 cctcttttgc ttgataagga atacgatgcc ccgtcacaag cagtcgatgt gtattgacca
    11761 tataaggagt acaggtaagc aaagtcacga gatctctgcc tttttggata cccaaagaat
    11821 ctacttcatc aggtaagacc acttggattt tttctacttg atacgccaat gtctttccat
    11881 tgatctggat gaaaaactgg tctccttttt tcaacttagg caaatctgta aataattttg
    11941 cttctgggag tccacgatgc ccagaaagaa cggcatgggt gtttttcccg ccagtcggat
    12001 aagaactttc ttccaataat gacgttcctt tttgcaaaaa tatttccgtt gtctgatcga
    12061 atataggtaa gctgacattg atttttggga tttcgatgac tccaagcata tgttccataa
    12121 aaaaggcatt actaggtaaa tcttttaatg cttttgcatc aactgcttgg ttgaaactgc
    12181 ctatccctgg tgatacgttt tgctctgcca actgttggtt tttctttgtc attttttcct
    12241 gctgtttttt tatttcctct gaattcttat tcgatgcttg cttttgataa tggatgatca
    12301 tttgctgggc aagaaaatca ttcagtgaat cttctacaaa aggataagaa aagatcccga
    12361 tcccaacgac taaaatgatt gccatgaaga tgtccagtat tcttattctg cttttattca
    12421 tcatgtccac ctattttcta atatgtatat gaagaggttg tgaaatcagc gccttgacct
    12481 gagtattcgc tcaacaaacg gcaaaatagt tcctcctatt ttgccgtttg ttgagcgaat
    12541 accaagagac cgaccacttg tcacaacctt tctccccatt tgaatcacta tcgtttattt
    12601 atcgtatgat ttttgtttcc aatagtttaa gcttctgttt cttgatggcg ttttttgaag
    12661 tagatcactg ctcctccaac taaagcagta cctaccaaga tgaatacgat gatccccata
    12721 ccaccagttg aaggcaagat tcctttaggt tggttatcga tcgttgtttc atcagaagtt
    12781 gttggatcat catctactgt aaattcagtg tacgcagctt cattcttagg taatgcgtag
    12841 ccttctggtg cttttgtttc ttgaaggcag tatgcccctt gtttcagccc ttcgattgaa
    12901 aatttccctt ctgaatcaga aatcaatttc acttcttccc agtcagaacc tgtgacttca
    12961 tctgaccaag catatgggta ttctcctgag gcaagatttg ctattttttt cattaattta
    13021 acagttggat cttttgctct tagaatagaa aattctgcat cggctaattt ttcttgcgtg
    13081 ttcacatctt gtttgatgaa gtggaaatca ccgtagtttt ggacattttc tacagtaatc
    13141 gtgttatccg tagttgtatt ttcccgattg actacaccgt ttggattccc aagattggct
    13201 tcatctgcgc catggatgat accggttgtt tgttgcaatt ttccgttttt tactataaaa
    13261 gtagtttctt tttgtggatt ggttaattga taaccatctg gtgcttttgt ttcaactaaa
    13321 gtataattgc cgtctattaa tccattgaca ctgatggatc cttcagtatc agttgtgaat
    13381 gtcaacgctg actcagcagg atcatagaga gcgacaccag tgttttcatc aaaaccggtc
    13441 acgaattgat tctctgaatt tttgatctgg aattgcgccc ctgctaactt atctacccct
    13501 gaaaagttcc cagctttctg gattttgata ttgctttctt tgatgacatt tttagcatat
    13561 aggtgaatcg tgtctaaagg tttgtcaggg tctgatgaat tcgggttata aaagggtaag
    13621 cttaacacga tatttggcga cgtttcgaca ccgggctttg gtgtttctac aaagacgtat
    13681 acagcatctt tcccatcaga tttacctgga agagtcaggt tagctaatcc ttcaccagaa
    13741 gtcgttacac ttgcgaaggg tgtagcgtct gctggaactg ctgtgtcttt acttggatag
    13801 tacgtagcag tgatgtcata tacgttgaat tcaacgccgt tcaacggttc gctattcgga
    13861 aatgtcaatt ccgttccgtt gtttatttga ttttctggtg ctgaagtgaa tttcaattta
    13921 tgcaaattaa ccgtaacatt ttctggtgta ggtgatacgg cctagaaacc tctgcagttc
    13981 ctaggccgag aacgaaagga agcagcatga tagcagttcc taaaaacgtc catatttttg
    14041 ttttgatgtt tttcatttta tttccctcct attgtttcct ttttgttgtt ttttataaaa
    14101 ccaagctgtc cctgttgaaa ggactagcaa caaccctaaa attgagaaac ctccaagcat
    14161 ctcttcgctt gtttttggaa agcttcctcc atttttttca tttgatggac catttgtctg
    14221 catctgaact ggcgtttttc cattattcgt cggatttgct tttggttcgt tgatgatttc
    14281 caatggtata ggatgaagcg gatcataact gcctttttca acttcaaaga gaatggttga
    14341 ttgactcaat atatatcctt taggcggttt aatctccact agttcgtaac ttccaaaaga
    14401 caatccatgg acagaaaatg ttccatcttt actggaagta aaaatcgttg ctcttgaatc
    14461 atctttggct acccaatgat ttatcccgtt ttcttgcgct aagtattggt catttttttt
    14521 gactagaaat tgagcatctg ctagtttttg tttcgtttgc cgatccactt tgacaaattg
    14581 tttgcctcct gtttggatgg caagatgtgt ttctactgtt tgattgtcag tcgtcaaacg
    14641 tacatcgtta tcaaatgtcg ttttttcact gttttctttc aatgtcattt ggtaacggat
    14701 cgttaatttt ttcccagcaa acgaactaag atcttttcca gaatctgata agacaaaacc
    14761 atgttttgtt ttttgtgtgt gaaatgtatg aatcttgtct ccaccaatca aaaaatcgat
    14821 cgtttctggt tctagccata actgaggatc agctgtatct ttgatcgtat aggttcctat
    14881 ctcgttgatg ttctcaggaa ttgttacggt gatgagataa ggaactttat ccccatctgc
    14941 aaaatctgtc tgcttattct ctactgtttt tgttaatgtg agtgcagcag gtattttttg
    15001 ttcgctcttt ggatacaagt ggatggtcgt caatttatga ttagatgaat ctgtcacagg
    15061 aagcacaacg acaaatggtg ctgatttttc ttttacttaa tctgaagtct ttatttccgc
    15121 aaatcggtaa acagcgtcac gaccttgctc atctttgtcc gatgcactaa aagaagcaat
    15181 gccctcttcc tcgtctactg tttttgtcac attttctgcc agtatttttt cactatcact
    15241 tttttgtgct agttttcttt gtgcgtcttc cacactactt ccttctgacc gcagcttgta
    15301 aaattcatcc gagacatcat atatagcgaa agtaacgccg ttcaaccctt ttactccttg
    15361 aaagttatcc gggatattta ttccgtcgtt ttgactgata cttggcatct tatcgctttc
    15421 gaacattcga ttatgcaaag taaatgtaat aggtgattct ctagctgaca cagtcggaga
    15481 acctataaaa aagaccagta ggataaaaaa gagaacagca aatatatcca cacgtacatt
    15541 ttttgttttt ttcatcgctg ttcacctgct tttttcttac gatggataaa ataaaagaag
    15601 ccagaaaaaa taaagatcag tcctaaaaag acaaagggca gtagacctat cccacctgtc
    15661 gaaggaagca agagagtcgg attgatcgtc aacttagcgg tatctgttgc gataatcgta
    15721 taattattgt ttacacttcc ttcgcctaaa ctaatcgtta ctatttgtga tccaatatct
    15781 gtccggctgt taactgttag ggtataatcc aatgattcgc ctaagacaac accggtaacg
    15841 tggagatttc caaccgaaat cgagtgactt tgggcatcat aattaaaggc agcgtcatcg
    15901 acagtaatca ctgcgttagc tttatagatg gtataagttc ctgatgtgtt ggctatatca
    15961 gtaaatgaca acttgggatt tgctgctttg actttgtcta ttccgctatt cgttagttta
    16021 attgtatagc tgccagcatt aaccatattt tttgctgatt gtgcatctgc tgtgtaatcc
    16081 atactgctaa gtgccacttg tactggagta agcgaactag tgacaccctc tgctagcttg
    16141 acatggattg acgggacacc tgcttttcca tcatatgtct tactgccact gagctctacc
    16201 gcaccgctga tttgcttaat cgtatagaca tcagatgtct gcacgatagt aaataaatag
    16261 tttgttctgg cagctagttt agctagtccg ctttttgtca attgccaaga cgtataggtt
    16321 ccaatttcag taggcgcagt tacttcaact cccttttcat tcatcgcttt gaaatcagtt
    16381 ggattccatg tgatttgatc aatactgaga tcattgttta aaagggatgt tgattctgtt
    16441 ttatcattaa aggcatattt aacgatcggt acaacagaaa gaccatcata gtacttactg
    16501 ccatttgcat tgattgatac tgttgcattt gtcttataat ataggatgac ttcagaaatc
    16561 tgttttccat ttgcgtcctt tacgattaca tttccatttg tatctaaact agctaaaaca
    16621 ctcattgctg attggttctt tgtttctgtc gctgaaacag ctgtaaaggt atatccgttg
    16681 atagtaggcg tattagtcgt caggtcttgt gcagcacttc cgtaagtaag attcattatt
    16741 ttagaaggag caatcatctg tccaaaatag tcttgatact taatagtgat tggtgtttgt
    16801 ttgcctgtat aggtcaccgt atcgacgtta ctttctaggg ctgtgaaaga accgtttgtt
    16861 tgtgcaaaag aatagcccgt tactgctgtt ggagtgataa ccactttgtc acccacatgg
    16921 attccagata atgtagtccc gtacgttccg atttttgctg ctccaccatt gactgtataa
    16981 gaaaaagttc ctgtccgatt cgcattgacc tgaaccgttg ctgcttgata ggttggaaaa
    17041 tttgaaacat ttcccttacc atcaacgatc tgcccatttt tgatcgagta ccccgtgaat
    17101 ggaatatcat ttgctaactg tataccgatt gaaaaagtac tcatacctcc tccggcagga
    17161 agccctcccc atgttgaggg tgtaaaagaa taacttttat ccgcattggg tgtaactgaa
    17221 tcgatcgtat ttccagcggt agccaccccc atatagatac tttttgggaa tgtgatagta
    17281 gtaccattta ctgtggagcc aataaacaga ttatggatat ctggacggtt atcaataaaa
    17341 gcgattgctt tggttacgcc ttgtaacgca gttttatccg cactcatcga tccgttaacc
    17401 gcaacaatga ctccatcttt ggcatatatc actgtatatc catctggtat ggtatgtccg
    17461 ttgaaattcg cagtggccgc aacagtacta aatgtctggc ttctacctgt ttctgaataa
    17521 gctttctccc ctgctgcttt tgctgctgtg atttcttttt ctgtcggatc aatcagttga
    17581 gtcgaggatg tggttgaggt tgtctcgttg ttgctagact gattggtgct ttctatggac
    17641 tgtatttcag aatagggtgg actatttgtc tcctctgtcg tttggttagc aaaatttgac
    17701 gtttctgtat aaacgtttac ataaatactt gataagctcc ctaagaaagt aaaaatcaag
    17761 atgatcgaaa tcataatggg atgtaagtat tttcttttag atacagcttt gagtctagaa
    17821 acaaaatgtt tttgacatat acgttggatt ttattttgag ttttcttaat cattttcatc
    17881 cctcctctca ataaaaaatc atcaaataat cctttacacg ttattctaa
//
LOCUS       DAYYGO010000058        17541 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000058 DAYYGO010000000
VERSION     DAYYGO010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17541
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.059"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..205
                     /locus_tag="ACHA5T_001633"
     CDS             <1..205
                     /locus_tag="ACHA5T_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887209.1"
                     /translation="FVLINKNDKNQGFLQFYGAAYAVLGVMGILTAFFNQRFIALVFL
                     LIVIIVSATFSIRFAKKIAEPKQ"
     gene            278..757
                     /locus_tag="ACHA5T_001634"
     CDS             278..757
                     /locus_tag="ACHA5T_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8887210.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            980..1123
                     /locus_tag="ACHA5T_001635"
     CDS             980..1123
                     /locus_tag="ACHA5T_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFM8887211.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            1468..1596
                     /locus_tag="ACHA5T_001636"
     CDS             1468..1596
                     /locus_tag="ACHA5T_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887212.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            1701..3284
                     /gene="abc-f"
                     /locus_tag="ACHA5T_001637"
     CDS             1701..3284
                     /gene="abc-f"
                     /locus_tag="ACHA5T_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFM8887213.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(3721..4905)
                     /locus_tag="ACHA5T_001638"
     CDS             complement(3721..4905)
                     /locus_tag="ACHA5T_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFM8887214.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(4930..5937)
                     /locus_tag="ACHA5T_001639"
     CDS             complement(4930..5937)
                     /locus_tag="ACHA5T_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8887215.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(6109..6468)
                     /gene="comGG"
                     /locus_tag="ACHA5T_001640"
     CDS             complement(6109..6468)
                     /gene="comGG"
                     /locus_tag="ACHA5T_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFM8887216.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(6440..6865)
                     /gene="comGF"
                     /locus_tag="ACHA5T_001641"
     CDS             complement(6440..6865)
                     /gene="comGF"
                     /locus_tag="ACHA5T_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFM8887217.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            complement(6828..7175)
                     /locus_tag="ACHA5T_001642"
     CDS             complement(6828..7175)
                     /locus_tag="ACHA5T_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887218.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            complement(7147..7617)
                     /gene="comGD"
                     /locus_tag="ACHA5T_001643"
     CDS             complement(7147..7617)
                     /gene="comGD"
                     /locus_tag="ACHA5T_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFM8887219.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(7592..7870)
                     /gene="comGC"
                     /locus_tag="ACHA5T_001644"
     CDS             complement(7592..7870)
                     /gene="comGC"
                     /locus_tag="ACHA5T_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFM8887220.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(7867..8916)
                     /gene="comGB"
                     /locus_tag="ACHA5T_001645"
     CDS             complement(7867..8916)
                     /gene="comGB"
                     /locus_tag="ACHA5T_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFM8887221.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(8873..9802)
                     /gene="comGA"
                     /locus_tag="ACHA5T_001646"
     CDS             complement(8873..9802)
                     /gene="comGA"
                     /locus_tag="ACHA5T_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFM8887222.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            9934..11688
                     /gene="ade"
                     /locus_tag="ACHA5T_001647"
     CDS             9934..11688
                     /gene="ade"
                     /locus_tag="ACHA5T_001647"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFM8887223.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNNQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(11786..12448)
                     /gene="trmB"
                     /locus_tag="ACHA5T_001648"
     CDS             complement(11786..12448)
                     /gene="trmB"
                     /locus_tag="ACHA5T_001648"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFM8887224.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12518..13300)
                     /locus_tag="ACHA5T_001649"
     CDS             complement(12518..13300)
                     /locus_tag="ACHA5T_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFM8887225.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(13504..14715)
                     /locus_tag="ACHA5T_001650"
     CDS             complement(13504..14715)
                     /locus_tag="ACHA5T_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8887226.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(14708..15445)
                     /locus_tag="ACHA5T_001651"
     CDS             complement(14708..15445)
                     /locus_tag="ACHA5T_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8887227.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            15583..16017
                     /locus_tag="ACHA5T_001652"
     CDS             15583..16017
                     /locus_tag="ACHA5T_001652"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFM8887228.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            16010..16354
                     /locus_tag="ACHA5T_001653"
     CDS             16010..16354
                     /locus_tag="ACHA5T_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFM8887229.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            16534..>17541
                     /locus_tag="ACHA5T_001654"
     CDS             16534..>17541
                     /locus_tag="ACHA5T_001654"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8887230.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
BASE COUNT         5603 a         3572 c         3101 g         5265 t
ORIGIN      
        1 ttttgtattg atcaacaaaa atgataaaaa tcaaggattc ctacaatttt acggagctgc
       61 atatgctgtt ttaggcgtaa tgggtatttt aacagctttt ttcaatcaac gctttattgc
      121 cttggtcttt ttactgatcg taattattgt ttctgccacc ttcagtatcc gatttgcaaa
      181 aaaaattgct gaaccaaagc agtaaataat tctctttttc cctaaagtgt tctacaataa
      241 aagagtacaa tacgattgac ttgaggagtg aatgcttatg ttacaacaat acaaaaaaat
      301 catggttgcc gtagatggtt ctgatgaagc tgaattagct tttaaaaagg cggtcaatgt
      361 agctatccga aataacggag aacttctatt agcacatgtc attgacacac gttctttcca
      421 aaccgtttca tcctttgatg gaatgcttgc agaacaagct actgaaatgg caaagcaaac
      481 attggcagat tatgaaagca atgcgaagaa agctggcttg aataatgtaa cctctgtcgt
      541 agaatatggc tcaccaaaac aaattattgc cagagaaatc ccagaagata atcaagtaga
      601 tttgattatg ttaggagcca caggattgaa tgctgtagaa cgtttattta ttggttctgt
      661 ttctgaatat gttattagaa acgcagcttg cgatgtgctt gttgtccgaa ctgatttaga
      721 aaaccaattg ccagctaaag accacaccaa agcttaacac gtagacgacc tgccaactat
      781 cggcaggttt ttttgcacaa aaaaccgtga taaaaacaaa agacgcggca agcactcatc
      841 ggtctttata atgtttaaaa taaagggaaa acagtcaatg agttcaagta aaataaaaaa
      901 aagtgaacac cagggcgatt agtcgccctg gtgtggttta aaacgaccta agcatgccaa
      961 agcttattgg ttcgtcaatg tggaagtttc cgatgaatct gatacgaaaa aatatgaagt
     1021 atcgatggcg gataccgatt ttatcgccaa actaaaattt tatttcgggt tcgggtaccg
     1081 cgagaaggca agtgctacat tcggtaaatc attcaagaat tgatgaaagg ctaccgcttg
     1141 ccattgcaac cacaacgata ctcctgaacg cgatgttggt gagatttcca aagagaagag
     1201 ctgtttaatg caacggtatt aagcaaatgg caaaaggaag tcatgtagac aaagtttttt
     1261 atcaaacacc atatgatttc gcctgaacgc tggtttagct cagaatacaa tgatgtggtc
     1321 cacgagacat taccctcagt cagataaaaa aagctctctg acagaattac acatcgataa
     1381 atattaagta aatgtcagcg ctagttgaac tttctgacac tttcaggtac actggaaaag
     1441 acaactacac gaaggaggga aaatactatg atgcgcatga ctaaaataac aagtaatgaa
     1501 aattaccaac gataccatat cgcatttagc catgcaattg gttttacagt tgctgttgac
     1561 gggacaagtt tgtccaaaac agttgaattc acgtaaaatc aatggccatc tcaaggtatt
     1621 tttctcatga taatctgagt ttaaataaca caggccatgg ataaccatgg cctttttgag
     1681 tggtctcaag gagagagatc atgatcatac aagcaatttc tattaaaaaa acaatcggcg
     1741 ggaatccatt atttgaggat ttatcctgtc aaattgaagc tggtgaaaaa attgccttag
     1801 tcggtatcaa cggaactggc aaatcaacct ttttacaaat cttgaccggc agagaaggtg
     1861 tcgacagcgg tgttatcagt cgcaaaaaag gcttaaagat cgggacggtc gaacaagaac
     1921 ttacagtttc cgaagcgacc gtcaaccact acttgctcca ttctgccgct gagatacagg
     1981 acctcaaaca gcaaatgagt cgctacgaag cattgatgac agaaccagat acaaacttag
     2041 agaagtgtct ggcccgttac ggtgagctgc agcatcgctt tgaagaacta ggcggttacg
     2101 tattagaaga ccggattgca gcgatcttgc aaggcttagg gattccacac cgtcaggaag
     2161 cacggttaac agaacttagc ggaggcgaac gagttaaagt cgctttagcc aaaattttag
     2221 cagccgatgc cgatttactt ttactcgatg aaccgaccaa tcatttagat cttaacagta
     2281 ttcgctggtt ggaaaactat ttacagaata caaaaaaatc ggttctggtc gtttctcatg
     2341 atcggcaatt tctcgatcaa gtcaccacaa aaacatggga actggaagaa ggtgcattga
     2401 ttgagtatcc cggaaactac agccgttttc gagtcttgaa agaagctcgc ttggctgagc
     2461 tgacaaaaaa ttacgaactg cagcaaaaag aagtccagcg gttgaaagtc atgattcgtc
     2521 gttttcgtca gtgggcacat gaaggagaca atgagtcctt cttcaaaaaa gccaaagaac
     2581 tggaacgccg cctggcgaaa ctgacactcg tcaaaccgcc acctcccccg aaaaatcggc
     2641 tgcaaagctt gtctaatggt ggaaaatctg gcaaagaagt tttcatcatt caaaacttgc
     2701 accagcagta cgctgatcaa gtactattca aagacagcag ttttgcagtc tatcgtggag
     2761 atcatttagc catcatcggc gacaatggtg caggaaaatc aaccttactc aaattattac
     2821 tcggtgagga aaagccttac ggtggcacca tcaaattagg tagtagcttg caaatcggtt
     2881 atttgccgca gcagctgcag tttgccgatc ccgatagccg tttgctggct tacgccatca
     2941 cattgactgg aaacgaagaa gtcgctcgac ggcaattagc caaaagtggc ttttatcaaa
     3001 gcgatgtggg taaacggatc aaagatttat ccggtggtga aaaaatccgt ttggcgttgc
     3061 tgaaactctt tttagaaaaa atcaacgtcc tgattttgga tgaaccaacc aatcacctcg
     3121 acagctatgc tcgagaagaa attgaagaaa tgctgcaaga ctatcaagga acgctcctag
     3181 cagtcagcca tgaccgctac tttttgcagc aacacttcca agaagcactc gttattgatc
     3241 aggagcagat cagccgccag ccgctcggcc aactgattga ctaggacgtt ttttcgcaac
     3301 aaaaaacttg attttcatac ctgtgctttc acgattcagg caaagtacag gtggaaaatc
     3361 aagtttttat tattcgatca atgaatgttt cggatcggtt ttctagctaa acataaagcg
     3421 aagaataaag tcgacagccc acgaggctga tctaaaaatg ttttttcttt ttacatatgt
     3481 aatgtccctt ataaagatga atgaaaataa aatattggga ttttcctgaa aatcaataaa
     3541 taaaatgcca gtcttttccc ttttgaccac ttttccaagc aaaattccct aagaagatcc
     3601 tgtaaaaaag aagggaaatt gaagttaaat taccattttg cggtcttctc cgcaaaaaaa
     3661 acacatgcaa gaattccctc ttgcatgcgt ttcatctttt atcctaacga atgctttggc
     3721 ttagccgcgc agacgctcca catcacgggc aatcattagt tcctcatctg ttgggatcac
     3781 taatactctt acttttgatt cttttgtaga gatatctact tcttcaccac gcaattcatt
     3841 caacgctgga tcgatttcac aaccaaacca agacatacct ttgatgattt cactacgtac
     3901 atgtccgtca ttctcgccga taccagcagt aaagacaatt gcatcaacac cattcatcgt
     3961 tgtcacataa ccgccgatgt attttcggat gcggtccacg aaaatgtcat aagctaaacg
     4021 aatatgttct ttttcatagt tcttttcaag atcacgcata tcactagaca atcctgttag
     4081 gcctagcaga ccagatttct tgttcaaaac attgatcatt tcatcaatat caatctctaa
     4141 tttttccatt aaatatggga gaacagctgg atcgatatca cctgaacgtg tacccattgt
     4201 caccccagct aatggtgtga atcccataga agtatccatg gatttacctc catcgactgc
     4261 tgtgatcgat acaccatttc ctaagtggca agtgatgatt tttagttctt cgatcggttt
     4321 gttcaataat tttgccgcac gttccgacac ataacgatga cttgtgccat ggaagccata
     4381 ttttcttact ttgaaatctt cataataaga caccggtaag ctatataaat aattttgctt
     4441 tggcattgtt gagtggaaag atgtatcgaa tacagccaca ctcaaaatgt caggtaaaat
     4501 ctttttaaac gctttgatcc ccattaagtt tgcaggatta tgcaaaggag caaattctgc
     4561 taattcagca atgcggtcca ttactttctc atcgatcact actgaatccc cataagtttc
     4621 tcctccgtga acaacacggt gccctactcc ggtgatttca tcataagagc ttaaaatttg
     4681 cagttcaatc aactgatcta acagcatttt tactgctact tcgtggtttt cgatatccat
     4741 gatttgttcg aatttttgat tatcgccgta cttgatggta aaaatagaat cttgtaaacc
     4801 gatacgttcg acgatccctt tcgcaactac ggtttcttct ggcatttgat ataattgcca
     4861 tttcaaactt gaactcccag cattgattgc aattgtttta gacatatgac tctccttctc
     4921 gttgaatgat tacagagcag atgatttcca gtttttaaac tcgttgaaaa attccgtcac
     4981 cttctctggt tcttttaatg aagcaagttt cacaagcaag acttctttta cttgttgtgc
     5041 ttgtggtcct tttttctgta agatcaaaat acttttttgc gattgtttat tacggaacaa
     5101 ttcatctggc aattgaatca ttccttgaag ataaccttct tctttaaacc attttttgat
     5161 ttcttcactt tgatccgttt ctaaaaatcc actcggcatc aaaaacaaac caaaaccatc
     5221 cggcttcaca tatttcattg attgctctag caataaatga tgtgcataac tatggccttc
     5281 ttcggtactt gttaaaaact cttttgcttt ttcgtcattc ggataataac cgattggcaa
     5341 atcgctgatt gcaaaatcga caggatctat taataattcc tgcaacccgt cttgatgaaa
     5401 gtattgaaca tttgcttgtg tcaaatcact tgtagaagct gcgacagcca acaatgtatc
     5461 atcaatatcc acaccaattc cttctgtttg gtaccccgca ttcgataggt ttaaaagaac
     5521 tgttaataac aagttgccca ttcctgccgc aatatccaga atcttaaccg gtgctttttt
     5581 atttgtgtat agttgttcaa taagaaaaac aaataaaaaa ccgatactat caggagtgag
     5641 ttgatggttc gcctgcaaat gttctgtctg gctgcccttc aataaaagta attgagacag
     5701 tctgcgccat tcttctggct cgaaagaaag tttcttcaac tcttcataaa gagctgtgat
     5761 ccgttgcgtt gtctcttttg tcggcactcc atccaccaca cgtacttgat aatcatctat
     5821 taaattttct gcattttcga cataggcttc taaaaaggaa gttcccaatg cattctgtaa
     5881 aagctgaata gcttccaggt tttgattaaa cccttgttcc attttttctg gtaacatggt
     5941 gcacctcatt tctagatata ttataacaat tttttcacct gtttaattct atcgaacaat
     6001 tgacaaaatt tcaatcgttt tcactgtttt ttatttgtga aacggcaaac atttttttat
     6061 taaaaacagt gttataacag aaaagagggc gttttcacga acattccatc attctgtact
     6121 agtatctgta tcatttttta cttcttctgt gaaccgaaag ataaacggtg agacttccac
     6181 ctctaagaat agggactgtt gtacccactg atatgttaca gatccagttg tgtagtaaaa
     6241 aactccttcc gaactttcgg caagcgtaga ctgttccgat aaagccatct gcttcattat
     6301 ttttgctata taataatttt tgattcgtat attaagatcc attgacaaac ggtagctttc
     6361 taatgctgcc atcaataaaa acgtacataa aagaaataaa agcaagacag aaaataaaat
     6421 agagccctta taacattttc tactgatcgt tttttgtcca tatggcatat ccatcttctc
     6481 cattttcaaa gtgtacataa aaagaaattg tgtgttcttt ttccaataat tgaagctcag
     6541 tgatacttgt taacataggt tgatgtccgc cggaagttct tcttttacgt atcattgcct
     6601 gatatctttc gatgatatac tgtttatcct cgatcgtgta gtgcagttgc tgatttttca
     6661 cagagacatt gtgactgctc ttgagttctt cttctaattg tgctaaaaat attagccact
     6721 ctttttgatc tttacgctgc aaaaattcac ttacttggat ggtttgctga atcaaaagat
     6781 tgaatgtgag caatagactg ctcaatatca tcagtccaat tagacattca atcagcgtaa
     6841 atgctggaat tacttgtttt ttcaatactg atcgcttctt tctccatatt gacaaacact
     6901 ttttcgatta ctccattatt atcgacaatt tggatatcgt atgggatcat ttcttccctt
     6961 cgctctggtc ttttgtgata catccgctgc gtcttgatat cttcataaat cattcgaata
     7021 aatagcagtt cctccccact tttcttttct gcctgaagca aaaacagctg gccaaagctc
     7081 actaagtaga cacagacaga agcgatcata agcgctgtaa gggactccaa caaaacaaat
     7141 cctgttttat tcaattcttt ttgtatagcg accactccct atctggaatt gatacgtaat
     7201 cgtccgctct ttttcttccc aataaaattg ataagctttt agtgaacttt cattcccggt
     7261 tctagcagca aaagtaatag aagcatggcg tttcaaggta atctcatcag gaatatctaa
     7321 tacttcccaa tttattttat ctggattcgg aacatgaaaa atgatttgat tctcttcact
     7381 attccaataa aagcctgttt ggatctgatt cacaatagcg attttttgcg tagaatagat
     7441 tcgtttttca aattggccga aaaattgata gactgccaat tcattcttcc atgcagaaaa
     7501 tgcgagaact ggaaaagaaa ggatcgttgt aagtaataaa atcagtaaga gtgattcaaa
     7561 caacgtatat cctgatgcta aaaccttacc ttcaaggccg ctgatagatt tctgcatgtt
     7621 ctcgtttgat atagccttca ttcaacaatt cctccacagt aggaactctt ccattatttt
     7681 gaagcgcata gagctctttt tgtgtgtcta ccaactggat gatcgcttca cgactttctt
     7741 ggtctacatg attcctataa cgcgacaaat tagggacgaa caacaacact aatacactga
     7801 tgatcagtaa gacgattagc atttcgatca atgtaaaacc gctataataa gatatttttc
     7861 tctttttcat ggcaaaacct cctctatgct tccgtaaaca ggtaataaaa cagcggcata
     7921 aactaataaa atcaaagccg ctacgcccaa aaaaatgatt ggctgaataa aatgcatcca
     7981 gtgatgaaga cgtttgaaaa gctgttttct tgtccattcg ctgtaaaaaa tcagttcttt
     8041 acctaaactt ccctttgcct ctccctgtaa aacaatttta ctgaattctt ctgatagaaa
     8101 acgataattc tggaactgct cagccaatga catacctgcc tccaatcctt gaatcaactt
     8161 agaggcaaga tgctgaatca gactttcttg tttcgtttcc tttaagcaga ctatgatttg
     8221 ccttaattcg aaaccttcat gaaaaagctt tccaatctct agtgacaaat aagccgattg
     8281 atacaaggaa tatatcggtc caatcaataa ttgtctgctt atccattcac tctgatcaac
     8341 aggagacaac ttcatcagac gataccttac tagtaaagcc aatagagtaa ccgctaggat
     8401 gaatagtaga acataccaat gaaagacttg tatcagctga ataccccaat gctcttgcat
     8461 gaccattcct gaaccaataa gctgaggcaa aacgaacaac cgcatactaa acaagatacc
     8521 tagcaaaaag gtgagcaata gcatcggata tgcaagcagc tttttcaaat cattgcgaaa
     8581 tccttcaaca agccgaaact gtttggcaat ttccaacaaa gttgtcggca gattcccatg
     8641 acgttcagct aactcgatct gtaccagttg ctctgctcca tatcctattt gcgaaagtac
     8701 ctcactaaac gcttgccctt cctttagctg atgttggata tttgccaaaa taacaggtgg
     8761 aaacgaacgg atcgtgagta ataaataaag ggattcttgc agactaaaac cagataaaag
     8821 cagttctccc atggtttgtg caaactggtt ttgctggctt cttgttaatc ctttagaata
     8881 gttctgactg ccaaatattt tcctgagctt cttcataact ttcctcccat gattttttta
     8941 ctgattcttc ctccataaat tgataactcc ataaaacaga aggatggtga ttggcatctt
     9001 ccaaaagttc ttgataaatg acaccagcta aacattcaga tagctcttct tttttctttg
     9061 tcagttcatg gattctgacc aatgtacctt caagattgcg tgcatgtacg gttgcaaaaa
     9121 cccggtgccc tgttagtgct gctcttaccg cagcatgtgc agttgattca tctcttatct
     9181 cgccaatgat tagcaagtct ggacgatgcc gtaatgcgag tctcaaaagt gtatcataag
     9241 tctgtgctat tttcggattg atctgtagct gcagaaaaaa cggttcttct atctctacag
     9301 gatcttcgat agtaatcact tgcaattttt cttccaaagc caaccgatac attagagaag
     9361 tcttgcctga tccgactggt ccgctgaaaa ggtataatcc acgtctgacg gttttctgta
     9421 aaatagttgc aaaatctcga gaactaaagc aacgatgggg tgtttcaaaa gtaccgtata
     9481 atagccgaat cactaaactc tctcgttgaa ggtaatctcc tacagaagac aaccgcagac
     9541 gaacttcctg tttttctaaa gaataagtaa atgcacctaa ctgagctttt cttttttctc
     9601 cgatatccat ctgtcccaaa tacttaaatc ttgagatcag ttgctgtcca tctgccgaag
     9661 agactttctt ccagaatgtt cgtttttttc catagcgaaa atagatgtaa aatacttctg
     9721 aagctggcaa tatatatacg tcttgggcat gtttggcaaa actttcactt atcatttgat
     9781 ccgataatcg ttttatttcc attattctcc cctccacaaa aaacatacgt ctttttgatc
     9841 tttttgtttc aaataaaatg aatttacaaa aaaatattgg caaaaaccga atttatgtta
     9901 tacttataaa aatttatctt gggaggcact ccattggaaa caacattaag gaaatatatc
     9961 gaagtagcaa gtaaaaaaaa gcctgctgac ctagtgatca agcatgctaa gatcgtcaac
    10021 gtctttacga aagaaatcat ggaaaaagat gtagcaatct gcgaaggaat gatcgtagga
    10081 atcggtgaat acgaaggaaa aagcgtacat aatgctaatg ggcaatatct agtgccagga
    10141 ttcatcgatg gccatgtgca tatcgaaagc tcacttttat caccaagcga attttcaaaa
    10201 gtatccttgc ttcatggagt tactactgta gtgacagatc cccatgaaat aggaaacatt
    10261 gctgggagta caggtcttga gtttatgata gaagatgcaa gacaaacact catgaatatc
    10321 ttttttatgc taccttcttg tgtacctgta accccttttg agaccaatgg tgcaaattta
    10381 aatgcaaaaa aattagcccc ttttttacaa aaacccgaag ttttaggctt agctgaagtc
    10441 atgaactatc aagcagtagc aaataatgaa acagacatcc tagaaaagat ccaattgatg
    10501 caccagcata agaaaaaaat tgacgggcat gcagctggta tcggcatgga agagttaaat
    10561 gtttatcctg cagcaggtat tcggacggat catgaagcta caactgcaaa agaagctaaa
    10621 gaacgtttgg atcttggtat gtatttaatg gttcgagaag ggaccgttgc aaaagatctg
    10681 gcatcgctac taccggcgat cacacctgaa aattcaagaa gatgcttctt tgtcaccgat
    10741 gataaactga tcaatgattt agtaaaagaa ggatcaatcg atcatatcgt ccgtcaagct
    10801 attgcacttg gaatcgatcc attacaagcc attcagatgg caacattgaa tgcagcagaa
    10861 tgttttgatt tgaaaacact aggtgcgatt gcccctgggt atcaagctga tttcttctta
    10921 acagacgatc ttaccaagct gccaattcat gaagttttct ataagggaac attagttgtt
    10981 caatctgggc aactccaaga ccatttattt gctgatcatt caaattcata tacgtgttgt
    11041 ctgccaaaac taaacgctca accattaaat ccacattcgt tagagctgct tctttcttct
    11101 tcacaagccc atgtgatcga aattatcccg aatagtctag tgactaaaga tttggtagaa
    11161 aaagtggacc gacaaggcga acgattcatt ccttcaatcg aaaaagacca attaaagatt
    11221 gcggtgatcg agcgtcacca tcaaacggga aacattggat taggcatagt gaaaggattt
    11281 cagctgaaac aaggagcgat agcgacaacg attgcccatg actcgcataa tctagttgtc
    11341 gttgggacaa acgacgagga tatgctgtat gctgctaatc ttttgattca aaaaggtggc
    11401 gggatgaccg ttgtcaacaa tcagcaggaa atagcttgtg tttctcttcc tataggcgga
    11461 atcatgacac aggaaccttt ttcgatcgtc tatgaacagt tgaatgaatt aataactaaa
    11521 gcacatcaac taggagcagc taaaaccttt gatccttttt tgacactttc tttcttaaca
    11581 ttatctgtca tacccgaatt gaagatcacc gacaaaggat tattttcatt ttctcgcttc
    11641 caattgattc caccttgtgg aaaaaccgat gaaataaata cattttaaat caaataaatc
    11701 ccagaaaata cattacatct gtaatgttca ttttctggga tttatttatg gatattaatg
    11761 tttttatgaa agcagaaagt tagtttcaca gacttttctt tttggtctta tcttgaaatt
    11821 gagcttctac tcgataaata cgttgacctt ttgaagaaaa tttttcttca tactccgtca
    11881 taatattgcc ttcaaattga ctttgatgta aatctaacca tacctgtttg aggatcatgc
    11941 catattgaga aaaacttgcc agagaatact caaacaatcc ttgattatct gttttgaaat
    12001 ggatttctcc atttggacga agaatctgtt catctacagc aagaaaagtc ttatatgtca
    12061 atcgccgttt ttcatgacgt ttttttggcc atggatcgga aaaattcaga tagatttgat
    12121 ctacttcgct gtcagcgaaa tactctgtca acgctgatcc atccacatgt agaagtttca
    12181 ggttaggaag cggttgttcg atcaacttgt ctagtgcaat cgaaacaaca ctgacctgca
    12241 tttctactcc aatataattg atttcaggat gggcttttgc cattccagtg atgaactggc
    12301 ctttccccat accaatctca atgtggattg gatgatcgtt ttcaaacaat tcattccatt
    12361 ttcccttcca caatgttgga tcactaatta caaaattcgg gtgagccgct aacatttccg
    12421 ctgctcccgg acgatttcgg acacgcatta tttatctcca ttcctttcct ctaattaaaa
    12481 aggtctggcc aaatctccgc agagtttggg ccaaacctca gccactgaaa attcgtttta
    12541 attgtaaaat ttcttcgttc atccgtcgat gatcttcacg tttgcaatat ctggtgattt
    12601 cttggagcaa attcatggcc gcataccaat gaaatttttc aagcatttcg tcagtcgggc
    12661 gtatgccata actgatcagc cactgattcc agctagacag tggcacataa tttcctaaaa
    12721 tcgttgcaat atccaccgca gggtctgcaa acatgattga atcccaatcg actagatata
    12781 aataattttt acatactaac caattacgat ggtttacatc tccgtgtact actgataagt
    12841 tttccttatc tattgacggg atctgctcac gaagaaaacg atggacaatt tgcagaaatt
    12901 gatttttctg gagttcttcc ggtaattctt tttcatattt ttgcagcatc ttctccggcg
    12961 tcatgacacg accaccgata cgggtcagca tggttttaag agaatttgaa tgatgtaaat
    13021 ggtacaaaac atcaatcacg tcgtttcttt gaccaatctc attaggagtc agcaagcgac
    13081 cttccagcca ctcttgtgct gtcaaagtat cgccattccc cgttcgtttt gtccaaacaa
    13141 gttttggtgt aattccttcc cttgaaagag cagccaacat cggagtcgtg tttcgtttaa
    13201 taaagacttt gtcctcatca cgcatgccta tgtatgtttt tcctgtgtcg cctttgatgg
    13261 gacgtaaccg ccactcttgg tctaactgaa actccatcat gcattctccc gtcctcttac
    13321 tctttgtttt gcagtactta ttattctagt cgcctgaaca ctacacgtca agcacatata
    13381 cgttcttttt catttaattg ccattcaaaa aaccagcttt tctcaaaaaa aatagaacga
    13441 acagcattct tactgtcaac gctctatttt tcattctcat ttttacccta cacagctttg
    13501 ttttcagtct gacatcttct tcaaacgagt tggtacatat agtttaacaa agagcatagt
    13561 aacaatcaaa taagccgcta gcggaaccca tcgctgctgc ccatctaata cgactgcagc
    13621 aatcaaacca aaaataaccg acgcgataat taatacccat gtgagtagat actgaatcgc
    13681 ctgcttcttc tgattcttat ggattggata aagctgaacg agaaccatat agcggaactg
    13741 gttcgctaaa ggtaacagtt ggaaaccgat caaatataaa aataagaaac cgataatcag
    13801 actaaaataa aagtcgacta ctccggcaat caataaccca cctataatta ctaatcggaa
    13861 ataaagacca ctaaaatcag aacctcttgc caatcttctt gcaaacaagt aaagataagt
    13921 gttttttgat tctcttttaa tatgagtcaa tataggatcc aaatagcgtc ttcttttgat
    13981 ctttgccgta atttcaggaa catctgtgaa taaattgata aattgataga tctgatgcaa
    14041 cctatgttct tctacatgca ccattttctc ccaatccaat cctttttctt gttgcttcca
    14101 caaaaaccag taatatagac ttgcctgtac taatgcacct gctattcctg cccatattag
    14161 ccaataaaat gatagaaaca aaaagatcag agaagaaaaa aaccagccta atttccaata
    14221 acgaataact tgttgatcta gtcgaaacaa ttctgctctc ttgatttgca gatgactgaa
    14281 tttcaaactc cataatatga tcagatagac gaaaaacatc gaaaaggatt gctccgtact
    14341 aaccaccact aatggcatgg ccaatccgca tgccaatatc aaaaagatca acggaaaaat
    14401 gcaagaatag cgaaaagctc gttccaaata ggtttgcatt tctttttctt taggaagtaa
    14461 aaaaacaata tctgctgcct ctgcaaaagt agccatctta cccacgaata aacagagcat
    14521 ccaaaataca gagattatga gccctccaat tgcaaacggt ggctgcaggg ttttcaacca
    14581 attagaataa tataatccca caccgccaat caagaaaaga caaactaaaa caaaatgatc
    14641 gttcaataca taacgcatat atttcatcat tttcttttga tgtcttgcta aacgtttttg
    14701 aaaaaaacta ctcatgtgcc tgctcctttg ttaaagcaat atagatttcg tccaaagaag
    14761 aatccggcag ctgaaattct tttctcaaca tctcaagaga accgtctgct cgtatctctc
    14821 cgttatgtaa gaccacaaaa cgatcacaat atttttctgc agtagctaaa atatgcgtag
    14881 acatcaaaat accggctcct tcttttttca tttctaccat caattcaagc aatgcattga
    14941 tagctagtgg atccaagcct aaaaatggtt cgtcgatgat atataaagaa ggctcgatca
    15001 aaaaggcgca taagatcatc actttctgtt tcattccctt tgaaaaatta gctgggaacc
    15061 agtcaagacg attagataac cgaaaagtct ccaacaactt ttctgctctt ttcattgcta
    15121 tatccatagg tatatcgtag gccatcgcag tgacttcgat atgttctctc aatgtcaatt
    15181 cttcatataa agaaggagtc tctggaatat acccaatttg tttgcgatat tcttccgtat
    15241 ttttctttaa ggatagatga ttgatttcta tcgttccttt tgaagcttcc agcagaccga
    15301 taatgtgctt gatggtcgta cttttccctg ctccgttcag tccaatcaac cctactatct
    15361 ctccaggctg tacatcgaaa ctgatatctt ttaacacagg gagatgtccg tacccccctg
    15421 tgacatgatc gataattaaa ctcattcttt ttcctccgcc atttattttt atttattata
    15481 ccacgaagaa aatgacggta ttttgttttt ttccttacaa tagctttaac ttatggtaaa
    15541 gttaagctga agtatttatt ctttatagaa aggatgtttt atatggaaaa ctgtattttt
    15601 tgcaaaatca tcaatcaaga aatcccaagc tataaaatat atgaagacga caaggtctat
    15661 gcttttttag acatctctca agcaacgaaa ggacacacat tagttgtgcc taaacgtcat
    15721 gtagcagata tttttgaata tgatcccgaa cttgcaggag aggttttttc gcgtgtgcct
    15781 aagattgctc aagcattgga aaaagctttt ccagaaatga aaggtcttaa tatcttgaat
    15841 aacaaccgtg aactagccta tcaatctgtt tttcattcac atatccattt agtccctcgc
    15901 tacagcaaag aggatgattt ttctattcac tttgtcaatc atcaagatag ttatggatcg
    15961 gaagaactaa aagctattca agaaacaatc gtaaagcagg tgtcctgcga tgattaaaaa
    16021 ctttttcaaa ggactgttat ttggagctgc tgctggcact gtcggaggtc tgttattggc
    16081 gcccaggagc gggaatgaaa caagaaagaa actgatcgaa gaattggaag aagcaacaac
    16141 tctgacaaat gatttaaata acagcctgaa tcattttaaa agcgcattga tcgaaacaaa
    16201 agaaacagct gaatctgtga ttcctgcttt ccaagaagca atcaaaaaag atatcgaaga
    16261 ttttaaattc caagccgaac cacgtatcac acaaatcaat gaacaagtag aaaaactaaa
    16321 agctgatctg ccagagtatc ctgaacaaat ataataatga ttattttctc aacagattta
    16381 actatataag tgagaaaagt ttgcgtattt cctaggaatg tgatgaaatt ttgaattttg
    16441 tttgaaacta ccacgacaac gtttttaaag gaaatgggat atggtatagt taatatataa
    16501 aaataataac taattacagg agtgcaaatt ttaatgaaaa aaaaatcaat tattttggca
    16561 gctactagtg cgttggctgt tttaactcta gcagcttgtt caggcgatac taataaagat
    16621 atcgcaacga tgaaaggcgg aacgatcact gtatctgatt tctatgatga agcaaaactt
    16681 gaatcatcga accaatcctt agtacaacgg atgatcattt ataaagtatt caataacaaa
    16741 tatggcgata aagtaaccga taagcaagtg gatgcagaat acgacaaaca agcgaaatca
    16801 ttaggtgata cttttgaatc acaattagaa gctgctggat atacgaaaga tacttataaa
    16861 gaatatattc gtaacaattt agcttttgaa gctggcttga aagctcacgt agatattaca
    16921 gatgacgatt tgaaaactgc ttggaaatct ttccatccag aagtagaagc acaaatcatc
    16981 aaattatcaa gtgaagatga agcaaaagat gtgaaaaaat cagcggatga tggagatgat
    17041 ttctctaaat tagccaaaga caaatcaact gatacagaga caaaagaaga tggcggtaaa
    17101 gttaaattcg actcaacgac aacaactatt cctgcagaag taaaagaagc agctttcaaa
    17161 ttgaaagacg gtgagatctc cgatgttatt actacaacaa atcctacttc ttatgcgaca
    17221 gaatactacg ttgtcaaaat ggtgaaaaac caaaataaag gcaatgacat ggataaatac
    17281 aaagatcaac taaaagatat cgctactgaa actaaactaa gtgacaatgc attcacaact
    17341 aaagtaatcg gtgaagaatt aaaagatgca aatgtaaaaa tcaaagatga tgcctttgaa
    17401 aatgtgttga gtgcattcac aacaacttca tcaagtacga aagactcaag tgaaactaca
    17461 gcttcaacaa agagttctga cacaaaatca actgattcaa caaaagaatc ttcaactgaa
    17521 gaaacaactg attcatcaaa a
//
LOCUS       DAYYGO010000059        17314 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000059 DAYYGO010000000
VERSION     DAYYGO010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.060"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(51..1598)
                     /locus_tag="ACHA5T_001655"
     CDS             complement(51..1598)
                     /locus_tag="ACHA5T_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFM8887231.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="ACHA5T_001656"
     CDS             complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="ACHA5T_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFM8887232.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(2043..2237)
                     /locus_tag="ACHA5T_001657"
     CDS             complement(2043..2237)
                     /locus_tag="ACHA5T_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887233.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
     gene            2320..2928
                     /locus_tag="ACHA5T_001658"
                     /pseudo
     CDS             2320..2928
                     /locus_tag="ACHA5T_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2948..3976
                     /locus_tag="ACHA5T_001659"
     CDS             2948..3976
                     /locus_tag="ACHA5T_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFM8887234.1"
                     /translation="MNRIWNKSHRNNCSLLLILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILIRNSEKESSPNLKLKKTYADQSDGRNVIKANLQNPNAS
                     YMFGLTTSVEIYKKETKEPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKG
                     AWKEKRWNLHTDFEITDDAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFI
                     LKERRKKK"
     gene            3973..4731
                     /locus_tag="ACHA5T_001660"
     CDS             3973..4731
                     /locus_tag="ACHA5T_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM8887235.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPNEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QNGTDSTGTATQVQNFVQVTDKSGIYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGTPSTISGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEGKA"
     gene            complement(4817..5557)
                     /locus_tag="ACHA5T_001661"
     CDS             complement(4817..5557)
                     /locus_tag="ACHA5T_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM8887236.1"
                     /translation="MKKYFIIEDNQFHSEWIIKELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            5618..6871
                     /locus_tag="ACHA5T_001662"
     CDS             5618..6871
                     /locus_tag="ACHA5T_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFM8887237.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGFIALMVVTLL
                     FPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGLRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            7166..7420
                     /locus_tag="ACHA5T_001663"
                     /pseudo
     CDS             7166..7420
                     /locus_tag="ACHA5T_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308281.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            7542..7868
                     /locus_tag="ACHA5T_001664"
     CDS             7542..7868
                     /locus_tag="ACHA5T_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFM8887238.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLQLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEF"
     gene            7936..8649
                     /locus_tag="ACHA5T_001665"
     CDS             7936..8649
                     /locus_tag="ACHA5T_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFM8887239.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYLVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PIFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEEKEIVTLITCGDMDAVTRLVVQGELESVTP
                     IKDATDDMRSAFNLETKTF"
     gene            8959..9811
                     /gene="licT"
                     /locus_tag="ACHA5T_001666"
                     /pseudo
     CDS             8959..9811
                     /gene="licT"
                     /locus_tag="ACHA5T_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
     gene            9932..11797
                     /locus_tag="ACHA5T_001667"
     CDS             9932..11797
                     /locus_tag="ACHA5T_001667"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM8887240.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            11811..13254
                     /locus_tag="ACHA5T_001668"
                     /pseudo
     CDS             11811..13254
                     /locus_tag="ACHA5T_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            13362..14063
                     /locus_tag="ACHA5T_001669"
     CDS             13362..14063
                     /locus_tag="ACHA5T_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8887241.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            14189..14701
                     /locus_tag="ACHA5T_001670"
                     /pseudo
     CDS             14189..14701
                     /locus_tag="ACHA5T_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(15099..15308)
                     /locus_tag="ACHA5T_001671"
     CDS             complement(15099..15308)
                     /locus_tag="ACHA5T_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HFM8887242.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
     gene            15549..16124
                     /locus_tag="ACHA5T_001672"
     CDS             15549..16124
                     /locus_tag="ACHA5T_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFM8887243.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            16124..16564
                     /locus_tag="ACHA5T_001673"
     CDS             16124..16564
                     /locus_tag="ACHA5T_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFM8887244.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            16580..17281
                     /locus_tag="ACHA5T_001674"
     CDS             16580..17281
                     /locus_tag="ACHA5T_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM8887245.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
BASE COUNT         5715 a         2968 c         3577 g         5054 t
ORIGIN      
        1 tacgtcaaat aacccgacct atagaataga atcgggaatg tttggcgttt ttagttacga
       61 gaaaaatgtt gttgtacatc cttgttccaa ggcaacagtt gatctaaagt ttctgctgaa
      121 agtacgggaa gatttggtat cttatcaaat agaaactcta gatactttac aggattcaat
      181 ccgttaagtt ctgctgtttt atatacactc agaatgattc ctgaagttcg tgctccttca
      241 agacttgtgg aatgcaacca atttttccgt cctatcacca gctctttgat atttcgctcc
      301 gcacgattat ttgtgagttc taaacgacca tcttcaaaaa taggttcaaa accacttcgt
      361 agtttacacg catattgaac ggctgcatcc aacttggatt ttgcaacagg atctatcgtt
      421 gccatccact cataaaaagc aataaataat ggacgcagtt cttgttgccg acagcttaat
      481 cgcttctcag gagaaaagtc tttccatttc ttttctagga caaaccattt atccaattgt
      541 ttgactgctt gagctgcggg aatatccgtg tttttctttc cttttggaat tgcttcgaaa
      601 aatttccgcc gaacatgcgc gaaacaatag acaagtcgta cactttccac ggcattatac
      661 ccgctatagc cgtcacagtg taaataccct gtaaactcct tgagaaaagt tttcggaaca
      721 tctccagcac gactttcgtc gtggtgataa taaacgatgg gatgctgact gtcttttcct
      781 gtacaaaact gccagtaata ggtatctgtt ttggcacttt caatcacacg ataacttgtt
      841 tcatccccgt gtagaatctc ttcttgatta agatagactc ttaatcgttc tcctaaaggg
      901 tctaacgcgt tttgcactgc tttaatatgc caattggtga tattatctct cgaaatatcc
      961 aacccatgtg tttgtttcca atagttctct tgccgatagg caggaacttt ctgaccaaat
     1021 ttcatccgga tcgtttccgc cactaaacta ttggaaccta agctattaga aatcaaaggt
     1081 ttaggaacga tcgctttttt aaaagaagaa agtccttctt tttcgcagct tttacaacaa
     1141 taggtatgtt gataatgggt gagacaatcc aacatagcct gatggaaacg aatttcttct
     1201 cgtacttttg tccgtccgat atctttcatc tgtgaaccgc aatgttcaca aaattgttct
     1261 tccaaaggaa ggagatgttc tttctctgtt ataggtaagt gtttgatttt gtctgctttc
     1321 gttcctacaa ctcgttgatg tccttttact ggaataatgg tggcagctgc ttctggaaca
     1381 cttacagaaa tttcttcatc actaaaaaga ttcaattgcc cattcgtttc ctcaaagaga
     1441 gtctctttag agcgtccaaa taatttgttg gttaaataat tgaccttttc attcaatagt
     1501 tggatttcag ttgtaagatt ctgaatggtt tgttgttgtg cttgattggt tttatgggct
     1561 tcttgaagca actgaagcaa cgtatctgta gtttccaaaa tttttcacca ccttgaatcg
     1621 aactgatttt gttacagaat accacaagaa acgcacaaga aataggggtt caacacattt
     1681 tttgtgttaa ccccccagct taaaaaactc ctttttttcc tggcaaaata gctcgaggtt
     1741 gatcgatact taatccttct aacaaccatc gaacctgttg gttcgttaag gttcgaactt
     1801 cgtctttcgt ccgaggccat tttaacttgc cgttttctat ccgcttataa cagagaagaa
     1861 aaccatcacc gtcccaatag agaagtttga aacggtcttg acggttcccg caaaataaaa
     1921 aaacggcatc ttcgtaaagc tcgagttgat actcttgttg aataagactg gcaagaccat
     1981 caattccttt tcgcaaatct gtttttccgc acacaatata aatatgtttg gtttgaagta
     2041 aatcataaag cattggacaa cagttctttc aaaatagctt gaagaatata attgttaata
     2101 ccgttataga tcaaaacttc agaaccattc tctcttttga tgcgcatcgc taacttggca
     2161 gccgcgggtt tacctttttc tggtgcactc ttttcgtttc ctaattggac aggaacaatc
     2221 tcatttaatt tatacataga aaacctccag acttcttgat agctcaagta tatcttgagg
     2281 tgcatttgtt agtaaggtac tgttctatgg ggcgcttacc cagaaaatca actttttgca
     2341 ggagatgtat tagaaattgt attaaacgac caagcaggag aagctgctgc taagggtgtg
     2401 cagaacaagc caagtaccaa cgatgaaatt gggaatcaca atccaagtga tacgccagtt
     2461 gtttatcatg atgccccgcc ttttgcacct gctacgaaga ttgtagtaga aggtggtctc
     2521 tcatttcttg ccccttccaa actcgattgt ggtcagatca aagcaactgg gttgacgcaa
     2581 gaagcatttg gccaaatgga tatacaggaa aaattatgga tttatgatca gcgagaaaca
     2641 aaaagtccat ggatcttaac gataaaatta tctcagcctt tcacaaccga tgcaggattt
     2701 tctatgcagg atgtcctata tttcaagaaa gctgaggagt atcaattagt gaatgaagaa
     2761 gaactggaaa ttgtcagagg acaaaattca acgaatgtgc aaacagatta ttcagaatat
     2821 ttaaataatg gtcgggcgtt taaacttgtc ttaaccaaag aaaatcagat agctggtaat
     2881 tactcagcag agatgacatg gacattagca gatgcgccga cagaataaag atcaaggaga
     2941 atgattgatg aatcgaatat ggaataaaag tcatcgaaac aactgctctt tacttctgat
     3001 ccttctcctt ggattcatcc tttggcagac acctgtccat gcctccgaat tcaattttgc
     3061 ggtagatttt gaattaccgg ctaatcagat tgataagaac aaaacatata taaatgtaag
     3121 gatggagcct gggaaaacac aggaaatccc ctacacggtc tttaaccgaa cagataaatc
     3181 agtaacagtt gagacattga tccactccgc tacgacgaat tcaaacggtg tgatcgaata
     3241 tggagaatca aaaagtaaat ttgatgaaac attaagttat gatatgaaaa acttagcaaa
     3301 aacagtggga gaaataactg tccctcctca aagtgcggtc actgagacac tttcactgaa
     3361 agctccagaa aaagagctgg ctggtgtggt cgcaggagga atcacgtttc aagaaaaaaa
     3421 agagaacgtt tctaacaaaa gtacagttgg tgtcgtcaat cagttttctc aagtgaaagc
     3481 aatcttgatt cgaaatagcg aaaaagagtc ttccccaaat ttgaaattaa aaaaaactta
     3541 tgcggatcaa agcgatggaa gaaatgtgat taaggcgaat cttcaaaatc caaatgcttc
     3601 ttatatgttc ggcttgacta catcggttga aatctacaaa aaagaaacaa aagagcctta
     3661 tctttcaata aaaaaagaaa aaatagatgt agcacccaat acaaattttg atttacttgt
     3721 cccattagaa ggaaaaaaat tagctccggg acattatcaa gcggttgtga agggtgcttg
     3781 gaaagaaaaa cgctggaatc tccataccga ttttgaaata actgatgatg cagcggacca
     3841 attgaatgaa aaggatattg atttaaaaaa tcagccagaa acatttgact ggcgatggct
     3901 gatacttgtt ggagtcattc ttattggctt atttgctgga ttatttattt taaaagaaag
     3961 aaggaaaaag aaatgaaaaa aacaattgca ttaagtaccg ttctagctgg aagtctatta
     4021 ttatgtacac aaagtgtttt tgctgagaat aagagctcag ccactacaga aggaacgatt
     4081 tctgtaacaa aaccagatgc cgcaaatcct gaagacgttg ttccagttcc tcctgttgat
     4141 ccaaatgatc ccgatacgcc gtttacacct aacgaaccaa atcagggaac atccggatta
     4201 ctgacgattg accaagctcc agatcttgat tttggaacaa tcaagttagg tacagcaaaa
     4261 acagtatatg ctgcattaca aaacggaacg gacagtacag ggacagcaac acaagtacag
     4321 aatttcgtac aagtaacgga taaaagcgga atttatgccg gttggaaact atcagtcaaa
     4381 cgaacagaat ttattggtac aacatctgat acgaataaac taactggttc aaaggtgaaa
     4441 ttcaaaaacg ccgtgattcg aaatagcgca actaatagca gtggaacgcc atctactatt
     4501 tctggtactg gcgctgaagg aattgagatc ccagcagatc aggcagtaga tctagtaact
     4561 gcaggagcaa atgaagggat tggcacatgg atttattcat taggagagaa tgtggaacaa
     4621 gggagacaat cagtggaatt atttgttcct gtatccaatt atgccgtaga caactacgtt
     4681 tcaacattca catggtcgtt aacagatgct cctacagaag ggaaagcgta aaatcaaatc
     4741 aatgacttca gggagtggag ccgattcaag gccaactccc tgaaatctgc tatgcttacg
     4801 tctcagctct tattcatcaa ctcgaagcag ttttttggaa gtcatggaaa cgttcctttg
     4861 tcttttcaaa gatctttcga ccaacgagta aggagtcacc attgaaaaat tctaacacat
     4921 aatgagcaga atcaattttc tgcaattgcg ataagttgat acagtaagat tttaatagta
     4981 aaaagtaagc atacttttcc aactctgtct tcaattgttt taaagaagta ttcactaatt
     5041 tatcttctgt agttgttttg accataactt ggtttttcaa acctttgata ctttcaatat
     5101 ataaaatttc ccgaggatac aggacgagac ttccaccttt ttgatcaata aagattttat
     5161 cgacatttac gatttgctgt tctttccaat tgatctgttc tagcacttga cgaatttgtt
     5221 cagataaatt cctgtctttt ctgatatatc caatcggaag gattttccga ttgatgatct
     5281 ctactgcaaa attcgtataa gcggtgaaaa aaacaatatt actatgtgca ttttttttac
     5341 gtatttgttc ggcgacatca atacctgtga gtgattgctt caagtcgata tcgataaaga
     5401 aaacatcgtg attatcaata gtacccaact ggatcaactg attgatttga gagatagaag
     5461 tgagctgcac gatattctcg atatgatgat tgaccaattc tttgatgatc cattcgctgt
     5521 gaaactggtt atcttctatg ataaaatact ttttcatatg caggctccta ttaaaaaaat
     5581 atgtgtacat tttgaaacaa aacaatggag gaataagatg gtcgcaacag tgacacttta
     5641 tttgaccttt actttgattt tagatttagg tatctattta ttcttttcga aacaactgct
     5701 agtaaaaaac agctttggtt ttatagcttt gatggtggtg acacttttat ttcctatccc
     5761 taaagtgagc tttccttacc tagttggctt attattgatg tttctttttt attttactac
     5821 gaaaaacctg tttatcagct gtttgatcag taatcttttt tttgctatca ctggactcag
     5881 cttttttctg gtttttgatc tgatggatta tttaggaaag agacatcttg gaacagcaag
     5941 tttttttgca ctacttatcg catttatttt ctatctatta gtaaaaagta tagacaaaca
     6001 gacgagattt ctcaacttgc tttttactta tccaactgaa cactattttg gaggtatttg
     6061 tattttatta agctggggcg tgatttactt ggtattggga agtgttcact tccgctcatc
     6121 taattatctg cttctatctc ttttgtcgat agtcatcagt tttttttctt tgtttttttc
     6181 cattatgttg atcagtttcc aagtaaaaga aagacaacat caggaatcgc ttcaactgta
     6241 tcaaataaat gaagaatatt accatcatct agaggaattc aaacatgatt ataaaagtct
     6301 actgttttca ttaaaaatta ctcttcagga aaataaaaaa gaaacgttgg aatttttaga
     6361 aatcttagaa gatcattctg cagctatttt tgaaaaaccg caaatacaac agctaacaaa
     6421 tattgtatcc ccagctgtca ggggtgtctt tttaaaattt ctagaaaaag cagaatcgga
     6481 gaaaatcccg atccaagtag ttattcctgt cgaagtgaaa acaatcccga ttgatctggt
     6541 tgtttttatt cgctgtttat ctatttttat ttctaacgca ttcgatcatc gtgtagccga
     6601 tcagagtccg attacgatca atctgctgga agaaaaagag ggattacgtt ttagtatagc
     6661 gaacaaggca aatccgacga atttgacgct atcggagatg gtcaaaagag gaaaaacctc
     6721 taaaaaaaat ggcgggttag gtctatacag tgcgaaaaaa atgttggatg tttacgaaaa
     6781 tgcagaacta aaaatcgaat tttcaaaaca gaatcactat ttttttgtag aaatgtatct
     6841 ggcatctaga aagagcaaat cagcctactg acttactgtc aaaggataag cttgaggaaa
     6901 gtctacacgg agatagagac aacaacacag catattcaaa agaaacagaa gcgtgatgac
     6961 cgttgtcacc cttctgtttc ttttgctatg tagttaaaca aagtagttga cacttccgct
     7021 acttagtatt agtacgaaag tatacttcat gatactaagt agataagaag tgaaaagcgg
     7081 aacccacgat cgtcaaaatg ctaactgctt tgcttacacc agataaaggg aagagcaata
     7141 tcgcttattt taggcgtgga gaagcaagaa aatccctacg atcacacaaa aaatgctgac
     7201 ccagcagtta agagagttag aacaagatgg cattgtccat cgaaaagtct ataaccaagt
     7261 cccgcctaaa gtagagtact cgctcaccga agaaggaaaa tcattaagag agattctagt
     7321 agcgatgtct gtttggggag aagaacgtat cgaaaaacaa aaagcctcag gggaagaagt
     7381 cacattactg catacacatg gtggctacct aaattactga tttctatgac atttatttta
     7441 aataaaagaa aacaggagtt tttttaaaag catcatctat tttttatgta aaaaaatggt
     7501 atgattaatc aagtaagaaa tgattggaag aaggcaagca aatgaatagc atggaaaaag
     7561 ttaaccagtt tagagatgaa cgtaattggc gacaatttca taacgaaaaa gatttggcta
     7621 tttctatttc tttggaagca tctgaattgt tagaactttt tcaatggaaa acgccggaag
     7681 aagtaaaaga aacacaatta gaacgaatca aagaagaact ggcggatgta ttgatctatt
     7741 catacatgat ggcagacaac cttcagctgg atctagatga gatcattgaa gaaaaattga
     7801 tcaaaaataa tttgaaatat cctgtagaaa aaagcgcagg tgtacgaaaa aaatacacag
     7861 agttttagaa atgtgtcatt cggctgttgg aaagagcaaa agaaaaacag taaaatgagt
     7921 ctggagggag agaaaatgaa aaaaaaagga aaagcaaaaa gatggctagt caatatcctc
     7981 ttgttcctct tgttattagt aggactagct ttgattttca atgaacagat caaggattat
     8041 cttgtcagag agacaggaga taaatatgct atagcgaatg taacaaaaga agatctgaaa
     8101 aagaataacg ataaagatgt cagttttgac tttgatgcgg tagaaccaat gacgacagaa
     8161 ggggtcatga ggtcgcagat gagaggaaca gatctaccag tgattgctag tatcgcagtc
     8221 ccttcagttt caatcaattt accgattttc aaaggcttgg ataatacgtc actactttac
     8281 ggagcaggaa cattatctcc agatcaagaa atgggaaaag gaaattatgc cttagcaagc
     8341 catcgtgcaa cgaaccctga attgttgttt acaccgctgg aaaatttaga gatgggtgcc
     8401 aagatctatt tgacagattt agaaaatgtg tatacataca aaacattttt taaagaaaaa
     8461 gttgctccta cagataccca attactgaat gaagtcgagg aaaaagaaat tgtcacacta
     8521 attacttgtg gcgacatgga cgccgtcacg cgactagttg tacaaggaga actggaaagt
     8581 gttacaccga taaaagatgc gacagatgat atgagatctg ctttcaatct agagacaaaa
     8641 acattttagg atactgggac ataagtcgtt acagattaaa aacagccaaa ggatatgaat
     8701 cgataaggaa aaaccaattt gctgaaaaaa agaggaacgc aaagaggtgc atctctgatt
     8761 tggttgtttt ctagatagac ttgtgttcca gtttttttat tttttatatg aaaaggcttg
     8821 cataaataaa aattcgatgg tatgatgatt ttgaataaga aatggattgt tactgtacgg
     8881 caggcaaaac cagaattcag ctaagaaaca gtaggcgaat tctaggtttt tttatttttc
     8941 ttgagagggc gaagcaaaat gaaaattgat aaaattttga ataacaatgt cgtcatttcg
     9001 aaaaatggat tcggggaaga agtcgtttgt atgggaaaag gactagcttt tcaaaaaaaa
     9061 gattggggac gagatttcgc cagaagcggt ccaaaaagaa tttattttaa gagattcttt
     9121 tgctaaaaac caattccagc agctgatggc tgatattccg gcagaggaga tcgaatgggt
     9181 caaacaagta gtcgaactgg ctgaagataa actaaaagtt gaattttcgc ctaatattta
     9241 tctgacattg accgatcatc ttcattatgc gatcacacga gcaaaagaaa atatccaact
     9301 gcctaatcca ttattatttg aaacaaaaaa attttatccg aaagaatatc aagcagcgaa
     9361 agaggctctt ttgttcgtga aacaaaagac gggtgtggaa ctttctgagg atgaagccgg
     9421 ttttattgcc tttcattttg tgaatagcca acaaggaaac gaagacatgc aagtgacgat
     9481 gaccgctacc acaatggtaa gagatatact gagtatcatc agtaaattct tcggcatcgt
     9541 gttcgatgaa gaatctttga attatcagcg tatcattact catctgcagt tttttgccca
     9601 gcgttatctc aaaggggaag gatctgacga gcaagatgaa tttctctacg cattagttca
     9661 aggaaagtat ccaaaagcgt ttcattgtgt cgaacggatc aatgagtatc ttcgacaaac
     9721 gcaaaaacag gaaatgggga tcgcagagca gatttattta acgattcata ttcaaagagt
     9781 agtaagtgag aaaaaaagca tccaatcata aggattgtta ctgtatggca ggcaaaacct
     9841 ggattgaaaa aataggttca cgaatgtttg tgactgtttt tttcaatctg ggtttttatt
     9901 ttttaaataa aaaagaaaga aggaaaaaac aatggataat caagcagttg gtcgtcgtgt
     9961 atgggaagcg gtaggaggac aaaagaatgt caaaagttta gtacattgtg ctacgcggtt
    10021 gcgattcaga ttaaaagatg aatctttagc agatacgcaa aagttaaaag aagacccgga
    10081 cgtgatccaa gtcgtacaaa gtggcggtca gtatcaagta gtgattggca gtaatgtagc
    10141 agatgtttat caatcgatcg tggatgagga aggtcttgca gtttcggagg atggaaaaga
    10201 agagtccaaa aatattttga atcgtctgat cgatattatc tctagtatat ttacaccttt
    10261 tctaggggcg atggcagccg ccggggtatt gaaaggattc ttatcattgg caacggtgat
    10321 gggctggctg acagctgata gcggtactta tcaaatattg tttgctgcag cagatggtgt
    10381 ctttaccttc ttacctgtca tgcttgcttt tacagcagct aaaaagttca aagccaacca
    10441 gtttttagca gtagcaattg ctatggcact tgtgtatcca gctatcacag cagttgctgg
    10501 tgcgggagaa gctatcagtt tctttgggat cccagtgatt ctttcaccat ctggttatac
    10561 ttcttctgtc ataccaatca ttctagccgt atgggttcaa agtaaattag agccattcgt
    10621 aaaaaaagtg attccgcaat ttctgcaaat gattttagtt ccgcttgtcg ttttagtagt
    10681 gatggttcca ttaacatttc ttgctttagg accaatcggg acggttgccg gtaatgcttt
    10741 aggcggtctc ttcaactcga tttatggatt tagtccaatc gtagcaggtc taatcatggg
    10801 aagtttatgg caagtattcg tgatgttcgg catgcattgg ggattcgtac cgatcatgtt
    10861 tttgaatatc gaacaatatg ggtttgatgt attgatgcca atgcttcttc cagccatctt
    10921 ggcacaaggt ggggctgctt tagctgtagc attgcgaaca aaagatacga aacttcgagc
    10981 attagggatt tcttctacag tgacttcgtt atttggaatc actgaaccaa ctgtgtatgg
    11041 tgtgacatta cctttgaaga aaccattcat tgccgcttgt atttctggag gtattggtgg
    11101 agctattatc ggattttccg gagtaaaagc tttctccagc agtttggtca gcttattgac
    11161 gattccgact ttcattaata cggtggatgg tgtagagtca aatgtgacag tagcagtaat
    11221 agctacagga attgcttttg ttcttgcatt cgtaggtaca ttgattttag gatttgacga
    11281 acaaacacaa gacaatcaat tagaaaacaa acatgcgaat gcaggagaac cgattacttc
    11341 agcacggcat accttaaaaa gtcctttaac gggtaaagta ctgccattga gtgaagtacc
    11401 agaccaagtt ttttcttctg gcgtgatggg aaaaggtatc gctattgatc cagaagtagg
    11461 agaactagtt gcacctgcag acggagagat cacgactatt tttccaactg gacatgctgt
    11521 agggatcaca acgactgacg gagcggagat tttgattcat atcgggatgg atacagtaga
    11581 attaaatggg aatggctttg agatcttagt caaacaaggg gatctcgtca aagcaggaga
    11641 tttattgatt cgttttgaca tcgaagcaat tagagcagct ggctatagcg taatcactcc
    11701 agttgtcatt acgaatactg atgcatttgc cgatatactt gaattagacc aaaaagagat
    11761 tattgcaaac gaagatgttc tggcaattgt aaaataagga ggaaatcagg atgcaatcac
    11821 gtttttcaga aaaattttta tggggtggag caactgctgc caaatcaatt agaaggggct
    11881 tatgatcgag acggaaaagg actatccgtt gcagatgcta tgcctggagg taagcagcgc
    11941 tttgcgatta tcggcagtga agaatttgac tggacgattg atcaagaaaa atatatttac
    12001 ccgaatcatc gaggaatcga tcattacgat cgtttcaaag aagatcttgc cttgtttgcg
    12061 gaaatgggat tcaaatgtta tcgtttttcg attgcctgga cacgaatttt tccaaatgga
    12121 gacgaaggta ctcctaacga agcgggatta gaattttatg atcagctgat tgatgagtgc
    12181 ttaaaatatg atattgaacc tgtgatcacg atttctcatt acgaaatgcc tcttcatttg
    12241 gcaaaagaat acggtggctg gaaaaatcga aaactgatcg acttctatga acgatttgct
    12301 cagacagtat tagagagata cagcagtaaa gtcaaatatt ggatgacttt caatgaaatc
    12361 aattcggcct ttcactttcc agctttaagt caaggattag taaaaagcaa tggagcagga
    12421 gagtaccaaa atattttcca agcatggcat aatcaattcg ttgcaagtag caaagccgta
    12481 aaaatcggcc acgaattgcg ttcggatatc caaatcggct gcatgattat ctatgcaaca
    12541 acttacagca tcgatgccaa tccagtcaat caggcagcaa caatgatcca aaaccaagaa
    12601 ttcaattttt tctgcacgga tgtccaagta cgtggagaat accctgcata cacagcgcgt
    12661 acccataaaa aatatggagt cgatccggaa aaattagagc aaacggaaaa agacttccag
    12721 ttgctaaaag aatatccagt ggactacatc ggatttagtt attatatgtc tacggcaatc
    12781 aatgagactg atccacaagc agcaacttca gaagggaact tgctgggagg agtaaaaaat
    12841 ccgtttcttg aggcaagtga atggggctgg cagatcgatc cagaagggct gagaatcgca
    12901 ttgaatgaat tatacaatcg ttaccagaaa ccattattca ttgttgaaaa cggtcttgga
    12961 gccattgatc atatggaaga ggatggcacg attattgatg attatcggat cgattattta
    13021 cgtcgtcata tcgaagcgat ggctaatgca gtagcagacg gtgtagattt gatgggctat
    13081 acgccttggg gatgtatcga tcttgtcagt gcatcaacag gagaaatgag taaacgttac
    13141 ggctttatct atgtggatct agacgatgaa ggaaatggga cactgaatcg atcaaagaaa
    13201 aaatcttttg attggtataa agaagtaatt gcctcaaatg gcgtaaactt gtaattaaag
    13261 cagctgaatg aaaaagaaca cacttctcac atgtgatttg tgttcttttt ttatttttta
    13321 tgtgctattc ttttagtaat agtaaagcaa agggtgacgt tatgaaaagg acacgtgtac
    13381 tatacttgga agtcgcagat aaaattaaag aagatatttt ctcaggaaaa tatccagtcg
    13441 gttcgatgtt gccgacagaa agtgaattag aagaactgtt tcaagtcagc aaaatcactg
    13501 tccgaaaggc aatcgaactg ctggcatcag atgaatatgt cgcaaagaaa agtggtcgtg
    13561 ggacgacggt tctaagcaat cgtccttaca atcgattatc caaagcggca agctttacgc
    13621 aaatcttgga gaactcccga tatgaagtcc ggaaagaaac gctggatttg caattggtcc
    13681 atataaagaa ggaagatcct ttgttttcct ttttcggtga gcaagctgtt tgttttaagc
    13741 gcttatatta tttgaacaat cagccttata tttattttgt tcattatctt ccacaagaaa
    13801 tgatgcaatt cagcagagaa gaatttggca aagaatcttt gtatcgtttg ctgacgaaaa
    13861 aaggatatac tattgactca ttttctgatg atttctcggc agtattttta acagaagaag
    13921 aacaaggaat tctgaaaaca gacgcacaga tcggcatcaa acggatacgg aaatcgtcta
    13981 cgcaaattgg ggaaatgatt gaatattcgg aggcggtcta ccatacggag ctgcatcctt
    14041 accatattga ttatgaaaca taaataaaaa aagaggttgt gaaaaagctg ccctgcatca
    14101 agtaataaga acagccaaac aaaaatagct tctcatattt ttcgtttggt taggcttatt
    14161 accgcagata cacggctgtt tctggattac catttattct ggataagggg gtgtggcacg
    14221 attttcagaa ggaatctatg aaccaatcac tgtattgatt ttagcttcga tggtaacccc
    14281 attcggcttg acgatcgctt atttcttagg gaaaatcatt cgtaaaaata tactcaatcg
    14341 tcaagaaatc gatacattga agaccgcttt tcctatgggg atctgtcaga tcacagaggg
    14401 atgtttccct atcgtattga acgacttggc acgcaatgtc atagcaacgg gagtcggtgg
    14461 gacagtaggt ggcggattaa gcatgttttg gggcgcagat agccgtattc cggcatcggg
    14521 aatgtttgca gtcgctacga tgaccagacc atgggcattc attggtgcat tgttagctgg
    14581 ttcttttgtc acaggaataa ccattttatt attgaaaaaa ccagtagatc cgaatgcaga
    14641 aatcatccaa gaagaaaaag aggaagaaga catatcttgg gatgatttga caattagtta
    14701 ggagaggcag aatggattac aagaaaaaag tacaatagat gtaccttgag acaggaatgg
    14761 tatgaaagaa tcagagttag aaagaatcga atatacagat tttggtttag gagcgatcga
    14821 aactgtccag agcatcggca ccctccttga aatgacgatg aaggaaaaat gagaaccttt
    14881 cgttgtcgaa agggtctggt ataccttatg tcgaaggaaa ggaaacaaaa aaactttcct
    14941 gtactattcc aaaaaggaaa gaaagttatt atacagtaaa aaaagaaatt attcttcatg
    15001 ctagagatca gtacacgttc gagcccaata tcaaacacta gttccaggca ggcgcaacag
    15061 gtgcggttat cactgaattt tcttccagca gtgacgattc aagtgatatt tttacaaatc
    15121 cagctattta tcgttaacaa aaagattttt aggagaagct gatgaacaaa aatgatcaaa
    15181 ttttgatcgt ttagttcgtc ggctttttta tttagagaaa catcatttat cagtgaaaca
    15241 taacacgaga acaaaatgat tttttcgtta ataaaagaaa caagtatttg ttgtttaaaa
    15301 taatacacgt tattggcata taggacgaaa tcccagtatc ttctataagt aagtcatttc
    15361 atatcgatga aaacacgtta tttatttttg ttttatctat aaaatggctg aaaaatgatt
    15421 tttttctaaa tactatatat agtggcgtct atcctatata ccactaaaaa actacaaaat
    15481 ctagtctttt attttaaaaa aacacgtgta gaatgaagat agacttttga gttagggagt
    15541 gtttttttat gaaacttact gttttttgcg gttcccgttt tggtaataaa gaaagttaca
    15601 aatttattgc gcaaacgtta ggaaaataca tggcacaaga agaaatcgaa ctggtttacg
    15661 gtggttcata ctccgggatt atgggagtga ttagtaaaac agtactggaa aataacggaa
    15721 aagtcacagg tatctatccg aatgggcttt ttgaaactga attgcctgga aaagatgtta
    15781 ctacatttat tcctacagaa acgatcgatg aaagaaaggt attattattt gaaaagggag
    15841 atgctgtcct cgttttccct ggaggtctag gtacattgga ggaattctca caacttcttt
    15901 cttggatagc aatcggtctg acgcccgaca agccaatcgg aatattgaat attggcggtt
    15961 attacagcgg cttgcaacaa ttattagaaa cttttgcaaa agaggatttt atggataaaa
    16021 aatggttgga tcaagtaatc ttttccaaca atccattgga actggtcaat gaattacgag
    16081 cagtatctat taaaaatcag ttattattga aggaggcaaa ataatgagtc aaatatattt
    16141 agcaggacca tttttttcag aggagcaaat cgatcgagta agtcgtatcg aaaaagcgtt
    16201 ggaggaaaac aaaacagtca caagtttcta ctctccacgt catcaccaag aaagcaatta
    16261 tgaactattc agcgctggat gggcgcagga agtatacgaa aaagatatgg aagaactgac
    16321 aaatgcagaa tttgttgttg ctatccttga ctttgagcat caaacaatcg atccaggaac
    16381 ggcttacgaa ttaggtgtgg caactatgtt gaaaaaaccg atgatcattg tacaagaaga
    16441 gacggtacct acgaacttga tgattacaca aagtctccat acgtatttaa aatcagacca
    16501 agctgtacgt gaatatgatt ttgaaacatt gcctgttgaa acttacgtag gcgagtactt
    16561 atagaagagg aagggttgta tgtcagtgat tttgatttca ggaacgattg gtgcaggtaa
    16621 gagcagtttg acggatatgc ttgcaaaaga gattgattct aaaccatttt atgaaaatgt
    16681 cgaagataac gaagtattgc cgctctttta ttcaaatcct gagcagtacg cttttttatt
    16741 gcaaatcttc tttttgaaca agcgattttt agcaatgaaa aatgctttag tgaacgatga
    16801 caatgtgcta gaccgatcca tctatgaaga tagcctgttg ttccatctaa acgctgattt
    16861 aggtcgggta acggatatcg aagtacagca gtacgatagc ttattagata cgatgctaaa
    16921 cgagttagat gatgttgctc caaaaaaacg gcctgatttg atggtccata tcaaagtttc
    16981 tttggacacg atgctagaac gtatcaaaaa acgtgggcgt gactatgaac agctagaaag
    17041 cgatgagacg ttatatacct attatgaaac gttgaacacc cgctataatc aatggtatga
    17101 agattttgat gtatgtccta aaatacaagt ggatggggat aaattcgatt ttgtagaaaa
    17161 tccagaagat gcaaaaaagg tgatccgtca gatccaagaa aaattagctg aattggaagg
    17221 aaagcctgct ggaaaatatt ttacggataa ccagcatgca aaaggtcaag aattaggcta
    17281 aaaataaaaa accaaacgac gggaaagaaa aatt
//
LOCUS       DAYYGO010000060        17134 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000060 DAYYGO010000000
VERSION     DAYYGO010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17134
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.061"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..371
                     /locus_tag="ACHA5T_001675"
     CDS             3..371
                     /locus_tag="ACHA5T_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFM8887246.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            551..1492
                     /locus_tag="ACHA5T_001676"
     CDS             551..1492
                     /locus_tag="ACHA5T_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFM8887247.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1561..2292
                     /locus_tag="ACHA5T_001677"
     CDS             1561..2292
                     /locus_tag="ACHA5T_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8887248.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2436..4616
                     /locus_tag="ACHA5T_001678"
     CDS             2436..4616
                     /locus_tag="ACHA5T_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFM8887249.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4636..5118
                     /locus_tag="ACHA5T_001679"
     CDS             4636..5118
                     /locus_tag="ACHA5T_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFM8887250.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5134..5895
                     /locus_tag="ACHA5T_001680"
     CDS             5134..5895
                     /locus_tag="ACHA5T_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFM8887251.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6026..7360
                     /gene="murC"
                     /locus_tag="ACHA5T_001681"
     CDS             6026..7360
                     /gene="murC"
                     /locus_tag="ACHA5T_001681"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFM8887252.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7667..8152
                     /locus_tag="ACHA5T_001682"
     CDS             7667..8152
                     /locus_tag="ACHA5T_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFM8887253.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8145..8828
                     /locus_tag="ACHA5T_001683"
     CDS             8145..8828
                     /locus_tag="ACHA5T_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFM8887254.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGIILAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8954..11599
                     /gene="polA"
                     /locus_tag="ACHA5T_001684"
     CDS             8954..11599
                     /gene="polA"
                     /locus_tag="ACHA5T_001684"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFM8887255.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11644..12480
                     /gene="mutM"
                     /locus_tag="ACHA5T_001685"
     CDS             11644..12480
                     /gene="mutM"
                     /locus_tag="ACHA5T_001685"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFM8887256.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12444..13073
                     /gene="coaE"
                     /locus_tag="ACHA5T_001686"
     CDS             12444..13073
                     /gene="coaE"
                     /locus_tag="ACHA5T_001686"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFM8887257.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            13125..13457
                     /locus_tag="ACHA5T_001687"
     CDS             13125..13457
                     /locus_tag="ACHA5T_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFM8887258.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13769..14260
                     /gene="nrdR"
                     /locus_tag="ACHA5T_001688"
     CDS             13769..14260
                     /gene="nrdR"
                     /locus_tag="ACHA5T_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFM8887259.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14283..15656
                     /locus_tag="ACHA5T_001689"
     CDS             14283..15656
                     /locus_tag="ACHA5T_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFM8887260.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15656..16582
                     /gene="dnaI"
                     /locus_tag="ACHA5T_001690"
     CDS             15656..16582
                     /gene="dnaI"
                     /locus_tag="ACHA5T_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFM8887261.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16711..>17134
                     /locus_tag="ACHA5T_001691"
     CDS             16711..>17134
                     /locus_tag="ACHA5T_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887262.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIID"
BASE COUNT         5836 a         3001 c         3783 g         4514 t
ORIGIN      
        1 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
       61 cgttttggag agcagttggc tttgcggaaa aagatcgtca agaaatggat gggacgctta
      121 taattgaagt aggagttgag gacagtcaaa cctcatttct gttatatgat cgagcatttg
      181 ttgagtccca ttcacctggc acagctacta gtgccccatc tttgatgttt tacagtaaag
      241 atatctttgg actatataaa aaaatgcaga cgcaatcagt cacattaggt gatatggtac
      301 agcttggaga agaatatgtc tttaactttg ctgatcccga tggcaactac tttgcggtta
      361 caggtaaata atagaaataa agactttgtt tttctgtgcg aaacaggaaa gcgaagtctt
      421 tttaattcaa ataataatat tatgtaaaca aaaaactttc catgcgtaat tatacacaag
      481 caaataaatc gtcaaatgcg aacgccttct ttatactgtt taggaacgaa aagaaggaag
      541 gaagaaatac atggattatc aagtactgct ttattacaat tatacaaaaa tcgaagaacc
      601 agaggaattt gccaaaaatc atttagcctt ttgcaaatct ttggatctta aaggtcgtat
      661 tctagtagct gaagaaggaa taaatggtac tgtgtctgga accgttgaaa acacgactgc
      721 ctatatgaaa gcaatgaaag ctgatcctcg atttgctgat acgatgttca agatcgatcc
      781 aagcgaagga catgccttta agaagatgtt tgtaagatac cgtccagagc ttgtaagttt
      841 aaagctagaa gaggacattg atccaaacca agtgacaggt gcctatcttt caccgcaaga
      901 atttaaagaa gctattttag atgaacagac agtggtgatt gatgcacgga atgattatga
      961 atatgacttg ggacattttc gaggggctgt acgcccagat atacgcagtt ttcgagagct
     1021 tccagactgg atccgcgagc acaaagaaga atttatggaa aaacgggtag tgacctactg
     1081 tactggtggc attcgttgcg agaagttttc tggttggctt gtccgtgaag gattcaaaga
     1141 tgtcggacag ctgcatggcg gtattgctac ttatggtaag gatcctgaag tccaaggaga
     1201 attatgggac ggcaagatgt atgtgtttga tgaacggatc gctgtcgata tcaaccaagt
     1261 agataaacaa atcgtcggtc gcgactggtt tgacggaaca ccatgtgaac gatatatcaa
     1321 ttgtagcaat cctgagtgca atcgtcagat tcttgcttcc attgaaaatg aagaaaaaca
     1381 tttaggagca tgtagcatag agtgtgcgaa acatccgcgc aatcgctatg ttcaagacca
     1441 tggaatctct ccagaagaag cagaactgcg gtttacagct ttaattaaat agaaaacaat
     1501 agttttgatt gtcgaccatt tccaatatgc gctatactag cgttgaggat ggtgatccac
     1561 atgaaatcat tgatttatgc tattggggat gtccatggca aacatgagct ttttgaaaaa
     1621 cttattcact attatgatcc aaagatccat cagcttgttt tattagggga tctcaatgat
     1681 cgaggtcctc gtacaaaaga atgtttttta ttaggaaagc aattagtcga agaaacagga
     1741 gccatttacc tccggggaaa ccatgaagaa tattttttgc aatttctgca atcaccggag
     1801 gactggtatg cgccttatat gagaaatggt ggaaaagaaa cgtttgaaag cctgattcac
     1861 ccaggggcat cggaggaata ctccccaact gaaatctcga tgatgatcca atcgagatat
     1921 tcagaactga ttaaattttt agtccagcgc cccttgtatt atgaatggga aaattatctt
     1981 tttgtccatg ccggtgtgga tttgacaaag aaagactggc gtaagacaag tctaagagac
     2041 tttatttgga taagggaacc atttcatgaa gggaaaaaca atacaggtaa gacaattgta
     2101 tttggacata cgattacacc aatgctccat ggagatatgc agacaacaga cctctggatt
     2161 caggatcata aaatcggaat ggacggtggg gctgtattcg gagggtcatt gcatggggtt
     2221 gtttttgaca aggaaggtat tcttcaagat attgaatatc agaacaacga aggaccttgg
     2281 catggaatct gactaatgga aagaatgaag gagctgtgac tttgttgcgg ctctttttat
     2341 ttttatagag aatattgttt ctttcagtag tttaacccag gataaaaagt atgatagaat
     2401 aaagaagcaa tttttagcag aaaagaggca ttttaatgac ggaaaatttg aaccagacga
     2461 tcattcagct tgtacaaaaa gaactttcag actatcgtcc taagcagttg actactgtac
     2521 ttaatttgtt aaatgaagga aacactgttc cttttatcgc gcgttaccga aaagaaatga
     2581 ctggaagtct tgatgaagtc cagattcgtg aaatcgaaga gcggtatgcc tatcttgaaa
     2641 acttagaaaa acgtaaaact gaagtgattc ggctaatcga tgaacaaggg aaactgactc
     2701 ccgagttaga agcagaaatc acccaagccg tgaaaatgca acaagtggaa gatttgtatc
     2761 gtccatacaa acaaaaacga cggacaaaag caacgattgc taaagaaaaa ggacttgaac
     2821 cactcgcgaa gtggctgatg cagcttacag atggagaggt gcaatctgaa gcagaaaaat
     2881 atattgacaa agaaaaagaa gtctcttctg ccgaagaagc gttgcacggt gctcatgaga
     2941 ttattgctga acaagtcagc gataatgcaa aatttcggac ttggattcgg tcttatacgt
     3001 ataacaaagg tatgtatgtc agcaatgtca aggatgagca agcagatgaa aaaggcgtat
     3061 atgaaatgta ttatgacttt gcagaacccg tccataaaat ggtttctcat cgaatattag
     3121 ctactaatcg tggagaaaaa gaagatattc tgaaagtatt ctttcaagta gatgaagcag
     3181 caattttagc ttacttggat cgccaaatag tcaaaaatcc tgcctctcct tcttcttcat
     3241 tcgtcagaga agcctatcag gatagttata aacgctttat tcagccagca atcgagcgag
     3301 aactgcgcaa tgaattgact gaaaaggcag atgagcaagc aatcgctatt ttcggcgaaa
     3361 acctccgtaa tcttttgttg caaccgccac taaaaggtaa agttgtcctg ggatttgatc
     3421 cggcttatcg tactggatgt aaactagcag tcgtagatgc gacagggaaa gttttagcaa
     3481 tcgaagttat ttatcctcat aaacctgcag cgcaagcaaa aagagaagct gctggaccag
     3541 cttttatcca attaattaat aaatatcaag tagatatggt agctatcgga aatggtacag
     3601 ccagtcggga atctgaactt tttgtggctg agcagttgaa atccgccgac cataaaacct
     3661 attacgctat cgtcaacgaa gcaggcgctt ctgtttactc cgctagcgaa attgcacgaa
     3721 aagaatttcc gcatctacaa gttgaagaaa gaagtgctgt ttctattgcc cgtcgcctgc
     3781 aagatccttt agctgaatta gtcaaaatcg atcctaaagc tgtcggagta ggccagtacc
     3841 aacatgacgt ttctcaaaaa cgtttggctg aacaattgga ttttgttgtg gaaacagcgg
     3901 ttaaccaagt aggtgtggat gtgaatacag caagcccgca gctcttgcag catatatcag
     3961 gattgaataa aacaactgcc caaaacattg tcatttatcg tgaagaaaac ggagaattca
     4021 ccgcacgaac tcaattgaaa aaagtgccac gcttaggacc aaaagcatat gaacaagcaa
     4081 tcggattctt gcgagtccct ggtgggaaaa acattttgga caacacaggt atccatcccg
     4141 aaagttattc tattgcaaaa gaaatattaa cttcagtcca actttcagaa aaagagcttg
     4201 gtacagaaga agcagtagaa aaattgacac gattatccgt agagaaactg gctgaaagcc
     4261 tatcagtcgg agaagaaacg ttagccgata ttttagctgg gctgacacaa cctggacgag
     4321 atatgcgtga tgagatgcca gcgcccttat tacgtacaga tgtattgagt atggaagact
     4381 tgaaaccagg gatggaattg actggaacgg tccgtaatgt cattgacttc ggcgcatttg
     4441 ttgatatcgg tgtgaagcaa gacggacttg tccatatatc gaaactcagc aaaaaattcg
     4501 tcaagcatcc tacagatgtc gtgtcagtag gagatatcgt aacagtttgg atagaacaag
     4561 tcgatacgaa aaaaggcaga atcagtttga cgatgctttc cccttatgaa gagtaacaat
     4621 gaggagtaac aaaaattgac acaagaagaa ctgcagcagt tagtagaaga gatatcatta
     4681 aaaagcttcc agcttccttt caagcaccaa gcaatgttca atccacgctt acgtacaact
     4741 ggcgggcgct atcatttaaa cgatcatcat ttagatttta atgcgaagct atttgaagag
     4801 tcagacgaag agacgattgc aggaatcatc aaacatgaac tttgccatta tcatcttcat
     4861 ctagctggaa gagggtatca gcataaagat atggatttca agtatctttt gaaaaaaaca
     4921 ggagggttgc gctatgcacc agccttgacg aagaaacaga taaagatgga gtattatcga
     4981 tgccaagatt gttctgccac gatctgccgc aagagaagaa tcgatgtacg caaatatcgt
     5041 tgtggaagat gtcatggaga aatcaaatgg attgagacaa aaagagaaga ctttaatgaa
     5101 gcacaaaaaa tcagatgaaa ggatagaagg cctttggaaa aaagtgagca gaggaatcta
     5161 aaagactgga atcagctatt aaatttttta gccgcacctg aagagggaaa ttttgaggaa
     5221 ctgacggcac ctacacttat tttagctgga aactgtcttc ctatcttagc tgataaagca
     5281 gcacaaatgt atttaaatgg ccaagtagat caattgtttt tggtcggagg ggtggggcat
     5341 gcgactagaa tcctttatga gaattttgaa aagcaaggat ttcattttga agatggcatg
     5401 agtgaaagtg aaatctgtcg tcagtatcta aaagaagtct atgatttgcc ggataaggct
     5461 tttctgatag aatcgaagtc tacaaattct ggagagaacg ccactttttc actggagatt
     5521 cttcattcct taggagctgt accagaaaaa atcttattga tgaatgatcc gacactgcaa
     5581 cgcaggactc gagcaacttt tgagaaagta tggcaaaatg aacaaactat tttcgcgaat
     5641 gctgtgccat ttgtgccgga aatccttcac ttttctgaag agatcatctt tacagcaaaa
     5701 gaactgaatc atcaatggcc taaggaatat ttccatgcgc tagtattagg agaaatggaa
     5761 cgtctccatg acgatgagaa cggctatggt ccgaaaggaa aagattttat tcctcacatt
     5821 gatatatcag aggaagtttg gtcttcttac accagaatta aagagtcaat aaaaacggat
     5881 ttttctcgta cttgaccatt ttagcaagcg aatcctcctt tccaaataaa tttggttgaa
     5941 attttcaatc gtttataaga taatgaggag cgtgttgaat gtaatagcta aaagaatgga
     6001 cgctagataa gagaagagga ttcaaatgga aaatcaaaat aaattgtatc attttgttgg
     6061 tatcaaaggt tcaggaatga gctccttggc tcttgtttta catgaacaag gtctaaatgt
     6121 ccaaggatca gacattgaaa aatatttctt tacacaaaga gatttagaaa aagcaaatat
     6181 cacgattctg ccatttaacg cagataatgt aaaaccaggg atgacgatca ttgcaggaaa
     6241 tgcatttcct gattcacatg aagaaatcca gcgagcaaaa gaattaggtt tggaagtgat
     6301 tcgctatcac gactttattg gtcattttat ccaaaattat acgagtatcg ctgtaacagg
     6361 ctctcacgga aaaacaagta cgactggact gctctctcat gtattgagtg gtgtccgtcc
     6421 aacaagttat ctaattggag atggaacagg acatggtgat ccgcaagcgg aattcttttc
     6481 atttgaggcc tgtgaatatc gccgtcattt ccttgcctat tcaccagact atgcaatcat
     6541 gaccaatatc gattttgatc atccggatta ttatacaagt atcgacgatg tatttacagc
     6601 tttccaaaca atggctggac aagtgaaaaa agctattttt gcgtatggtg acgatgctta
     6661 tttacgcaaa ttaaaagcaa atgtgccgat ttattattat ggtgtcacag aaaacgatga
     6721 tatccaagca cgaaacatcg aacgcacaac ttctggttct gcttttgatg tctatcatgg
     6781 ggatgaattt gttgggcact tcactgtacc agcattcggt aaacacaata tcttaaatgc
     6841 tttaggtgtt attgcagtag cttatttcga aaaactggat ctgaaagaag ttgcagaaga
     6901 aatgctgact ttcccaggag taaaacgtcg attcagtgaa aagatcgtag cagacatgac
     6961 cgtagtcgat gattatgcgc atcaccctgc agagatcaaa gcaacgattg atggcgcacg
     7021 ccaaaaatat ccagataaag aaataattgc agttttccaa ccccatacgt ttacgcgtac
     7081 gattgcattg atggatgaat ttgcagaagc acttgattta gctgataaag tttacttatg
     7141 cgatattttt ggttcggctc gggaagaaca aggaaacgtt aagattgaag accttggagc
     7201 gaaaatcaaa aaaggtggag aagtcatcaa agaaaataat gtatctccat tgttagatta
     7261 tcatgatgca gtagtaatct tcatgggtgc tggagatgtt caaaaatttg aacaagcgta
     7321 tgaaaaactt ttaagcagca cgacgaaaaa tgtactgtaa tacaattact gcagtgtcct
     7381 tataaaaata gaaagagggg gagtggcaaa aatcttgcca agcccccttt tttgcaactt
     7441 atgataaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaatcaa atcaaaaaca
     7501 tgagaagctg ttttttcttg gctgttctta ttatttgatg caggacagct ttttcacgac
     7561 ctctgtaaac tataggaatc tgttaaatca agggagcaga acttgccttt tacgctactt
     7621 ttggtacaat aatgtatgat taaaaataaa ctggagggtt tctatcttga acataggtaa
     7681 gccgagaaaa ctaggaaaaa caataaatga aatcgaggaa ggcgactcgt tatccttaac
     7741 agaatctatt gaagataacc agcttttgct gtatttggga ttgacgaatg atgcaaatcc
     7801 tttgtatatc caacatgact ttgcacaaaa aacagaatat caacagccaa tcgttccatc
     7861 agtgatgctg atggggatca tcacaagtgc aatttcaaag catcttcctg gaccaggatc
     7921 acatgttgtc aatttttctg tcaattttat tgaaccggtt ttccattatg aaacactgac
     7981 ttttcagttt gaggtcataa aagtagataa gatgaaggat gtggtgacga tttctgtaga
     8041 agcggagaat acgaacgaag atcgtgtgtt ggatgctgtc gttatggtcc aaccgccaca
     8101 agttcctgaa ttggaaatag caatcgaaag tgaaggtgaa ttgaatgaat aatccaagta
     8161 catcaatgtc aggtatcaat cgtttttatg caaaagttta tggattttta ggtatgggac
     8221 ttgcccttag tgcagtcact tcttacttgg ctctttgggt atttcctttg caattagcaa
     8281 cttttatcaa taattttcca ctaggctttg tgggtctgtg gatcgttgaa ctgattttag
     8341 taatggttct aggcgtaaaa gcacaaaaga atccgagctt agcaatcggt ggatttattg
     8401 cctactctct tttgaatggc ataatattag cggttactct tgcatactac gatattggaa
     8461 cagtaacgca agctttcttg acagctactt tgatgttcat ggggatgtct gttgtcggag
     8521 taacaacaaa aagagatctt agcggggttg gaagagcagg acaggcagct ttaatcggag
     8581 tgatcattgc actagtcttg aacatcttca ttttgagaag tagcgtagtt gagctgttca
     8641 tctccctact gttagtcgtc atctttgcag ggattacagc ttatgataac caaagcatcc
     8701 gtaatatcta tgtccagaca aacggaacag cacatagtgg aattgctgta ttcatggcat
     8761 tgaagctgta tttggacttt atcaacttgt tcttggcgtt tctgagaatc tttggacgga
     8821 acaactaaaa aagaaaaaaa ttcatcagtt ggggctatac cgaattattt ggtatggtcc
     8881 cgctttttct tttcgcattc cgcaggattc tttgttagaa tgaataagaa ctttacagta
     8941 aggagttaag aagatgacaa aaaataaatt attactcgtt gatgggaaca gtgtggcttt
     9001 tcgcgcattc tttgcgcttc acaattctct tgaacgtttc aaaaatagaa acggtcttca
     9061 tacaaatgca atctatgcat tcaatacgat gtttgaaaat gttatgcaaa aagagcaacc
     9121 gactcatgtt ctggttgcgt tcgatgcagg aaaaacgact tttcggacag aaatgtatgc
     9181 ggagtacaaa ggtggaagat ccaaaacacc tggcgaattc aaagaacaaa tgccatatat
     9241 acgtgatttg ttgacaggac taggggtaca gtattacgaa ttgcccaatt atgaagcaga
     9301 tgatattatc ggtactttgg cagaaaaagt agacaaagat caatttgatg tcgtcgtgct
     9361 ttcaggtgat cgggatttga ctcaattagc tagtaaagaa gtaaaagtgg atatcactgt
     9421 aaaaggtgtc agcgatatcg aaagctatac accagaacat gtagcggaaa aatatgatgg
     9481 gttgacgcca aaacagatta ttgacatgaa gggactagcc ggagatgcct ctgacaatat
     9541 ccccggtgtg actaaaatcg gtgaaaaaac agctatcaag ctattgaaac aatatggctc
     9601 agtcgaagga atttatgaac atatcgatga aatgaagcaa agcaaaatga aagaaaatct
     9661 gataaatgac aaagagcagg ctttcttatc gaaaaaattg gcgaccatca atacttcagc
     9721 gcctgtggat gtctctatcg actcattgaa gtatgaaggg aaaaatctcg ataaactcgt
     9781 tccgttttat aaagaaatgg atttcaatca gttcctttct aaattgaata tcgtagaaga
     9841 agaagtaaaa atggatgata ttttgtttga agtcgttcat gaattcaaag aggaaatgtt
     9901 tactacggat atggcgttgt acgtggaaat gatgggcgac aattatcata cagaggaaat
     9961 cgtcggagta gcgtggggga cagaaaagaa aatttatgtc acgaacgatc tatctgtatt
    10021 cgaaaaccga gcgttccatt cttggatcac tgatccgact cggctgaaaa aagtatatga
    10081 tgcgaaacga acttatgttg ctttaaaccg ctatactgga aaaacgaaag gaatcgattt
    10141 tgatgtcttg ctaggtgcat accttctcga tacgaatgaa aaaagcacag atatcgaagg
    10201 aatagctgct cattacggat acaacgatat ccaatcagat gaagctgtat acggcaaagg
    10261 ggcgaaaaaa ggtttaccag aagaagagga actctttttt gctcatttag cgcgaaaagt
    10321 agctgcaatc aatgcgttga caccaaaatt gagccaagaa ctggcagaaa aaaaccaaga
    10381 agatcttttc cggaaaatgg aactgccatt gtctatcatt ttggcagaga tggaaataag
    10441 cgggatcaaa gtagatgcga ctcgcttaca agaaatgaag ggcgagttct ctgctcgctt
    10501 aagagagatc gaacaaaaaa tctacgaaga agccggcgaa gaattcaatt tgaattcacc
    10561 aaagcaatta ggagtgatct tatttgaaaa aatgggatta cccgttatca aaaaaacaaa
    10621 aactggatat tccacagccg tagatgtact ggaacagctt cgtgaacaag caccaatcgt
    10681 agaggatatc ctcacctatc gtcaaattgc aaaaattcaa tctacctatg tcgagggact
    10741 gctgaaagtg atccaatcag atggaaaagt gcatacccgt tatgtccaga cattgaccca
    10801 aacaggacga ttaagctctg ttgatcctaa tttgcagaac atcccgattc gattagatga
    10861 agggagaaag atacgtcagg catttgttcc tagagaaaaa gactggctga tttattcttc
    10921 tgactattca caaatcgagt tacgcgtact tgcgcatatc tcaaatgatg aacatttgaa
    10981 agctgccttc ctagaaggtc aagatatcca tgccagcaca gcgatgcgtg tatttggaat
    11041 cgaaaaggca gaggatgtca caccaaatat gcgtaggcag gcaaaagcag tgaattttgg
    11101 tatcgtgtat ggcatctctg attacggact ttcacaaaat ctaggaatca gccgaaaagc
    11161 agcacagcaa tacatcgata cgtactttga aaaataccca ggtgtcaaag aatatatgga
    11221 aagaatcgta cgcgaagcaa aagaccaagg ctacgtcgaa acgctgtatc accgccgtcg
    11281 atatctgcca gacatcaatt caagaaatta caatatccgt tcttttgcgg aacgaacagc
    11341 tatcaataca ccaatccaag gaagtgctgc tgatattttg aaaatcgcga tgatcgaatt
    11401 ggataaacga ttaaaagaaa caggtcttca agctacgatg ctgttacaag ttcatgatga
    11461 acttgtcttc gaggtaccgg aaaaagaact tgaatcatta gataaattgg tgaaggaagt
    11521 tatggaacaa gctgtttctc ttcatgtacc actgatcact gacagcagtt ggggaaaaac
    11581 gtggtacgaa gcaaaataaa cattgtttaa gacagagcaa tataatgaag gtgaaaagaa
    11641 aaaatgccag aattaccaga agtagaaacc gttcgtaaag gattggaacg gttagtagta
    11701 ggaaaaacga ttcaaaaagt tcaggtcttg tggcccaaga tcatcgagca accagagaca
    11761 cccatctttg aggcttctct tgttggcgaa accatccaat ccattggacg tagagggaag
    11821 tttttaattt ttcatctcga tcattgtgaa ctgatctcac acttgcgaat ggaaggaaaa
    11881 tatcaattta cgaaagaaaa tacaccaatc gataaacata cgcatgtact tttctttttt
    11941 gaggatggaa gccaacttcg ctacaacgat gtacgaaaat ttggtcgtat gacaatcgtc
    12001 gaaaaaggag caagtgcaac ttaccgagga atcatgaaat tgggacctga gcctttgcca
    12061 gatagcttct tattagcaga tttcgcaaat ggactgaaaa agtcccacaa agcaatcaag
    12121 ccattgctgc tagaccaacg tctagtgact ggtctaggta atatttatgt agatgaagca
    12181 ttatgggaag cgaagatcca tccagaacaa ccggcgaaca cactgcgccc caaagaggta
    12241 gaacaattac gtctatcgat cattgatgtt ttggatcgtg caatagaagc cggaggtacc
    12301 acgattcgca gttatttgaa tgcattagga gaatcaggcg gatttcaagt agcactgcat
    12361 gtctatcagc aaactggcaa accatgtata cgttgtggaa cgccgatcgt gaaaacaaaa
    12421 gtggcacaaa gggggacgca ctattgtccg aattgtcaaa ggctaaaaat cgcaaagtag
    12481 gatttgtttt aggtttgacg ggtggaatag ctaccggtaa aagtacggca gccaaagtct
    12541 ttcagtctca tggatttcct ttgatcgatg gcgatgtgat tgccagagaa actgttgagc
    12601 ctggaacgct tgctctgagg aaaattgtat cagtgtttgg ccaagaggta ttgcagcctg
    12661 atgggcaact agatcgaggg aaacttggga tgattgtttt tccatcaaag gaaaaacggc
    12721 aaaaacttga tcagctatta gatccgttta tccgaaaagc gataaaagat caaatcgcct
    12781 ctctaagttc caatcatcct ttagtaattg tagatatccc attgctctac gaaggtcatt
    12841 atgaccacta tatggatgca gtagctgtcg tttatactac gccagaaact cagcttcagc
    12901 gtttgatgaa acgtaatcaa ctgacgctag aacaagcgca gcagagaatt agtagtcaac
    12961 ttccaatcga agaaaaaaag cagcgtgcgg atattttgtt cgacaacaat ggaacaaaag
    13021 aaatacttgt gggacaaatc gaaaattggc tttttgctaa tcgctttctt tgagtatctg
    13081 tgtcaatttt gctatcctga gtatatagaa agtgagggat agcgatggga tcattaaaaa
    13141 atcaaaagga taaagtcgta ggtaacgcaa aagaaaaaat cggcgagatg ctcaataaag
    13201 aaaatctaat cgatgaaggt cgtacgcaat cagaaacagc agaagaaaaa gaaaagcagt
    13261 accaagcaag aatcgatgaa atagaagacc agcaggcaga agaagaagcg aagcggcaag
    13321 atttgactca agaacgaaaa aatccgacac ctgatcgtag taaaacaccg ctcccagaaa
    13381 gtgaccacat tcccgatgag caaaaaatcg aggaacagcg aatgaaacac tatacaggaa
    13441 tcgaagagaa gttataaaat agtaaaaaat cgagatgtga cttgaagcag atgagaaggt
    13501 gttcatctgc ttcaagtttt ttgtgctaga tgactaaatc agcttttgtg taagttattt
    13561 ctcagtaatc aaggtgatca gtattggaaa attgaaattt agtaaggaca ggggacacaa
    13621 ttacgcatga aagtagaaaa aattgttctt atatccgtgt tttttgggta ggacagtcgc
    13681 gtttgtgatt tgtttcgtgt tataatacaa ctagttgtaa agaaagacac atcctttata
    13741 taaaatgcga tgcaagaggt gagacaaaat gagatgtccc agatgtcaac ataataattc
    13801 acgagtaatc gacagccgtc aagcagatga tggacgtgcg atccgtcgcc gtcgcgaatg
    13861 tgagaattgt tcgttccgat tcactacatt tgaacgaatt gaagctgctc cgttattagt
    13921 aatcaagaaa aacggggaac gcgaagagtt caatcgcgat aaaattttaa gaggactcat
    13981 ccgttcagca gaaaaaagac ctgtagctat ggaacaaatg gaacaaatcg tcaaccatgt
    14041 cgaaaaccgc gtacgcagtc tcggagaaaa cgaagtatcg acgaatctaa ttggtgaata
    14101 tgtgatggaa gatttagttg atttagacga aatcgcttat attcgttttg caagtgttta
    14161 tcgccagttc aaagatatgt ctgttttctt aaaagagttg caagatatcg tggataaagc
    14221 aaaagaaaat aataaagaga ataataaaga aaacaattaa gaaacaaaaa ggaggcattt
    14281 ccttggaaaa tgcttggaaa gagcttcaac caaaaaatat ttaccaagga agaaaagcgg
    14341 ctccgctgag agaagacagc aagaacgcct tgctgtatct ttatcagcca ctaatcggcg
    14401 gagaagcctt gagtatctat ttcaccctgt tatcagaaat ctctcaagaa tctggcaaag
    14461 gtcctgaagg gatgcatgca gatctgctta gtagtctagg ctgcggtatc ccgcaatttt
    14521 atgatgcaag aaaaaagcta gaaggaatcg gcttactgga tgtttatttt aaagaagata
    14581 gtgatctggg acctcgtttt atttatgagc tgttagaacc actcattcct aaagcatttt
    14641 tcaaagactc tgttctggct tttcttcttc tggaaaaagt tggtgaacga cgattccaga
    14701 agcttaccca attctttgaa cctaaaaggc atacagcaga gggatatcaa aaaatcacaa
    14761 aaaaattttt agatgtttat cgcttcaatg aagcagtgta cgctgctaat caagaaaaca
    14821 tcgaatcgct gaataaatct tttgaagaat ctgaatcagt agcattactt gaagcagatt
    14881 cgacattcga ttggcagttt ttgaatgatt ggctggcaaa aaaacatatt gcaagtcttg
    14941 atgatccaat catcaaacaa gtcgacatgt atcatcagct ttacggtttc gatgaattga
    15001 cacttggcga actgatcagt caatcttatg actttagtga acaaaaagta tctgaaaaag
    15061 aactgcaaaa agccattttg gcaatgcaga gacgtccggt gaagcaaaca aaagaaacag
    15121 ttgcggtttc tgaaaacagt aatgaaccct cgtcaaacga acatttgaca aacgcagcgc
    15181 ttcagctgat aaaagaagca cagagcattc caccgatgaa atatctggaa gcaatcaagt
    15241 cagaaaaagg cggatatgtt tctaaacaag aaaactggtt actgcaagat cttgtttctc
    15301 aatcaggtct ttccagtagc gtgatcaatg ttttgctgaa ttatgtacta gtgatcaaga
    15361 ataatgctag cttaaatgcc agttatgtaa atacagtggc aaatgaatgg gcacaaaaga
    15421 aaatcgccac agcagaagaa gcgatccagc acattcggca aataagtaat caagcaaaac
    15481 aatcaaaaca aaagaagcaa acaaattatc aaactggcaa gcgaaatgtt cgacgggaaa
    15541 agcttcctga ttgggtcaac cagccaaaag atgaaaaaca gatcagcagc gagaaaaaag
    15601 cagagattga ccgccgtttt aaagaatacc tggcaaaaaa ggagggtgat atctagtgga
    15661 agacgtaggc aaagaattga agaggatcat cagcaaacgc aattatcaag aaaagtatgc
    15721 tgaaatgatc gctgaagtct taaatgatgc ggatgttcaa gcttttctaa gcgcacacca
    15781 agaagaattg acacaagcgg atattgaaaa aagttacgct aaattgtatg aattcgtgca
    15841 agaaaagcgt aaatatcaaa aaaacgatcc aacgatgatc gctcccggct atgagccgaa
    15901 gctgacgttg aattttcatt atgtggatgt cacttatgta ccaacggaag cattgttagc
    15961 acggcaaaga caagaagaaa tcagaagccg agtaaaagca atggatatgc caaaagatat
    16021 tcaatcggct tcttttgaaa actttgaacg tacagatgga cgaggatatg cgagcttaga
    16081 agccctagat tttgtagaga aatatactgc tgatccaaaa gggtatcata aaggactgta
    16141 tctagtagga agttttggta ttggaaaaac atatttatta ggagcaacag cgagagaatt
    16201 agcgattgct ggattcacca cgacgctagt ccattttccg acatttgccg ttgagatgaa
    16261 acaggcaatc ggaaaagatc aagtggcaga aaagttagat gcagtgaaac gttcacctat
    16321 tttgatgatc gatgacattg gtgcagatgc gatgagtagt tggatacgag acgaagtatt
    16381 cggtgttatc cttcaatatc ggatgcagga acagttacca accttcttct catcgaattt
    16441 tacaatggat gaattggaac agcatttgtc cgtcactcaa cgtggagatg aagagccgct
    16501 aaaagcgaaa cggataatgg aacgcatacg ttatttgaca aaagaaatcg aaatgactgg
    16561 tcgcaaccga cgcaatggtt gaacagttga caagtccaac tgacatttga cctagtagta
    16621 aaagcaggtc agcgaatcat tatatggaca gaaatgaccg gagacaatca ccgcaacaag
    16681 taatgcagaa acttaaacgg aggatttttt atgagggcta ttttttctag tatatctgta
    16741 gttggtctat tttttctttt agcaggctgc ggtagcgcag atagcaacac aggttctgtt
    16801 gattcgattg atgcgggtca aacagtaaaa acaagcagta cgaaaacgga gagacaaagc
    16861 atatttgtcg gaacgattga aagtatcact caagtagaag cagaagaaga aacgcttcag
    16921 ttgaaactcg tcgatgtcat ggccaaagaa gatccagcga atatgggaac gtcattcagc
    16981 aatgacggag tgatcttaaa tgctactgct gaaaaaataa gcgtggacat aaatgaattg
    17041 aaaatcggtg acaaagtcca atttacatta gttgagatgc cgatcatgac gatgtctatc
    17101 ccaccgcaag ttcctggaga ttcgattatt gatg
//
LOCUS       DAYYGO010000061        16983 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000061 DAYYGO010000000
VERSION     DAYYGO010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16983
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.062"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(200..1510)
                     /locus_tag="ACHA5T_001692"
     CDS             complement(200..1510)
                     /locus_tag="ACHA5T_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFM8887263.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1522..2940)
                     /locus_tag="ACHA5T_001693"
     CDS             complement(1522..2940)
                     /locus_tag="ACHA5T_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8887264.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3180..4748)
                     /locus_tag="ACHA5T_001694"
     CDS             complement(3180..4748)
                     /locus_tag="ACHA5T_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFM8887265.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4752..5162)
                     /locus_tag="ACHA5T_001695"
     CDS             complement(4752..5162)
                     /locus_tag="ACHA5T_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFM8887266.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5159..6082)
                     /locus_tag="ACHA5T_001696"
     CDS             complement(5159..6082)
                     /locus_tag="ACHA5T_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFM8887267.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6079..6957)
                     /locus_tag="ACHA5T_001697"
     CDS             complement(6079..6957)
                     /locus_tag="ACHA5T_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFM8887268.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6944..8281)
                     /locus_tag="ACHA5T_001698"
     CDS             complement(6944..8281)
                     /locus_tag="ACHA5T_001698"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFM8887269.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8284..9387)
                     /locus_tag="ACHA5T_001699"
     CDS             complement(8284..9387)
                     /locus_tag="ACHA5T_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8887270.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9461..10969)
                     /locus_tag="ACHA5T_001700"
     CDS             complement(9461..10969)
                     /locus_tag="ACHA5T_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8887271.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10935..11627)
                     /locus_tag="ACHA5T_001701"
     CDS             complement(10935..11627)
                     /locus_tag="ACHA5T_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFM8887272.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11649..12623)
                     /locus_tag="ACHA5T_001702"
     CDS             complement(11649..12623)
                     /locus_tag="ACHA5T_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8887273.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12923..13795
                     /locus_tag="ACHA5T_001703"
     CDS             12923..13795
                     /locus_tag="ACHA5T_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8887274.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14377..16575)
                     /locus_tag="ACHA5T_001704"
     CDS             complement(14377..16575)
                     /locus_tag="ACHA5T_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8887275.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            complement(16731..>16983)
                     /locus_tag="ACHA5T_001705"
     CDS             complement(16731..>16983)
                     /locus_tag="ACHA5T_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8887276.1"
                     /translation="TEGFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVPHSNG
                     VTEGLNNKIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
BASE COUNT         5004 a         3552 c         2779 g         5648 t
ORIGIN      
        1 tttttttcga tagaatcaag cttcgcaagt ggttcttttt aacgacttag ttatcttatc
       61 acgtctaaaa acatgtgtca actctttttt ggaattttca taaaatgttt caatgaagcg
      121 cgacaacgtt gtttaatata tcattaaaaa aaataaaggt caagctaaaa tattatttaa
      181 gcttgacctt attataattt caacgattgt caaaagtatt gtttgcagct gtgttgccag
      241 tttctataga taaatcacag cttttgaaac ataagttttg tactgcttcg attcgcccga
      301 attcttttgc agttatttgg caatgagaaa agcagacatc ttctataggc ttttcaggaa
      361 atgctactaa atcaaaagct cgggaaggaa gtgagtaacc tggagaaagg catgcctgta
      421 cgtttttgat gtgtatgtct ttgactttcg tcatttgctt tgattcaggt atttcttcag
      481 ccactgcttg ccaactttct ggcatttgag caaggtcatt gctatttttt ctattgtatt
      541 cattatgcca gtccattatc caagaaaaag gaaattgtac atcaatcata ttcagatttt
      601 ctaccctgat attttcgatc actccccctc tttcttttga agatttcatt cgaataccac
      661 agtctgactg aaaaaaatcg atatcgtgaa tatatacgtc actaactcca gcactaactt
      721 cgctaccaat agttacaccg tatcctgaac ggattttaca tctagcaacc tcgattcttg
      781 atgattttcg attgactctt gcaccattgc catctctgcc agacttaata gcaatacaat
      841 catcaccaca ggccaaatca cattcataga cacgcacatt tgttgaagaa tcaatatcaa
      901 tcccatctgt acttggtcca tcattgttgc ggatgattac tttttctact aatacatcgt
      961 tagaataagt gatttgcaag ttccaaaaac ctgatttctg aaaaatcaaa tcacgaacaa
     1021 caacatgttc tgcatgatac agtaaacaag cgcgtggacg tttaatagca taatccgcaa
     1081 tccaacgaag tcccttttga tcgtattcag ctcttgtccc gcttttttga tcttttcccc
     1141 aatacagctc ccaccaatgg tctccttgac cgtcgattat tccttttcct tctataagaa
     1201 tatctttcgc cgagagaaca ttcaaaatcg ctgctggcca tttcatttct acgccggcta
     1261 ctcgtgtatc gatttctgga aatgcttcga tatctgttct ccccttgagg actgcacctt
     1321 cttcaaagat aagatgactc ttatctttta aaaaaagggc tccgactaaa taaatgcctt
     1381 ttggaataaa tacaggtaag tgttctttcg caccttgatc gattgctttt tgcagtatta
     1441 ttgtatcatc ttgttgagtg tcgtttgttt taaagttcaa agcattgata atcccttttt
     1501 ttgtccgcaa tacgtttctc cctatctttc attgattttt ttcaattctt caccaataaa
     1561 tacagttttt gaacgattca gtgggtaacc tactagcaaa atgattgcgg atacaaataa
     1621 gcagaatgct ggtaacaagt ttgccatcgt ataaatcctt tcttgtacta aaactgattg
     1681 gatggctcca cctgttgtag acgattggta accaatgagt gccagcataa atccgcctac
     1741 cgctcctgca catgcttgtc ctacttttct cgcaaaagaa tttactccat atactgttcc
     1801 atcttctctt aagcctgtct tatattgatg ataatcaatc acgtcagtaa tgaatgccca
     1861 gaccatcaaa ttgaataaac cagatcctaa agtagcgata aataaaagaa tcagatatac
     1921 ccatgcattt gttatatgaa ggaaatacat cagcaagtac atacatgaag aagcaagtaa
     1981 cgcaaatacg cttgcttcct ttttaccaaa taattttgtc agctttgtag caaatggtgc
     2041 taaaactact actgttccgt aagtaaataa aagagcaaca gacattgctt gtttattatg
     2101 gaagtaatca ttgaataaat aagtcatatt cgtcccagct aaaatctgat tgatcacaat
     2161 gaaaatgtca acaatgacta aaataatcag tgctcgatta gctaacaaac cttttaccaa
     2221 ctgagaagca gatacttttt cgtttttgac aactcttact ctttcagtag tcaatttaca
     2281 agttaacata tatgcgacaa atgctattgc agcacatccg attgaaatcc aaaagaaatg
     2341 attccctgaa acgacttgtt gtccactctt gttatgggta tacattaaaa tcggaatgaa
     2401 gaatcctgta gttgatccgc caatcgcaga accaatgctt cggaacgtgg acaacgaggt
     2461 gcggtcatta ggatttgagc taattgctgc tgccattgct ccatacggga tattgattgt
     2521 tgagagaaaa atcccataaa caatgtatga aacaaaaata taagcaatct tcaccgtcat
     2581 tggaagatgt tggacgaatg gcaaaaacaa aatgacagtc actaagcaaa atggatactt
     2641 catttttctg atccaaggaa taaatcggcc agtttctgtt aactctgaga catcacataa
     2701 tcttccaacc atcacatcag caaacgcatc caaaattctc gaaatcaaga ataacgtacc
     2761 tacgattgcc cctgaaacgc caagaacatt cgtataataa atcatcaaga aactattgac
     2821 taaacctaaa ataaaacagt tacctagatc accgaacatg tagcctatct tatcggaaat
     2881 cccaaattcc tttttttctt tttttattgt atttgctgta acagcatttt gtgaaagcat
     2941 ttgaattcct cctacctctt ctagtaaacg ttttcaaaat tataactctt tttttcttgt
     3001 ttttatattc acaatgttgg ctttttattg cattatcttg acagataaaa aacaaaaaat
     3061 aatctaaaaa cattgatgca taagattgat aacttttttt acataacaat atatacaaac
     3121 aaaaaaattc actaactagt gaaaataagt tagtgaacct catttattaa agaattaaat
     3181 tacagatgat cttttccatc atacttgaaa aactcaaaat cagcataact tcctcttttt
     3241 ttatttagat catgcactgc tatcccgata aaatttccag taaaccctcc tgagagtata
     3301 cacatgtttt tttgcggacc tactttcttc cattcttcag ttgatacgtc tttcaaatga
     3361 aactgtgctg tctctttctt tacagtgact ttcaaaaaag tcttcttttc tgtgagtgga
     3421 atcgtccaat cttctatttg tgctactccg ttttctgatt ctatcatacg taggcatttt
     3481 cccttgtcct cattccaagt aacataacaa taaatataat tggattcatt taagtacagt
     3541 accagtccag ccatctgatt aaaattatta ggcatgtatt caaatgctgt ctccgcatca
     3601 aaagtaaaat ccgtttgccg aatagctaac aaatgatgtt cgaaggtaga ttgaagagat
     3661 tcaccggaaa tcagacgtaa aaaacctggt ctacattttg tatcacacca atcagcattt
     3721 ggcaaaatgc gtctggcatt ccagttcttc tttagctctc cttcaaatgt atccataaac
     3781 tgatgatctt tttcttgttg tacctcttca tttgtctcaa tcattatttc ttccatcgga
     3841 gttgtctggc tatctttaag tcttaaccaa tcgtcggacg tccaatacac ttcttgaata
     3901 gcggtttctc ttcccaaaat agccgcaccg tttaatggtc tggtacaaag atatgtcatg
     3961 taccatcttc ctgacggggt ttgtactaag ctcccatgtc ctgtacattg caatgtagaa
     4021 gcaggtttgt cactagctgt cagcatagga aaaccaggat caagctcgta aggaccagta
     4081 atattctttg ctcggcaaac tgtaactgaa tgtcctcttc ctgtaccgcc ttctgccgtc
     4141 atcaaataat aatacccgtt acgaaaatac aaatgtggtg cctccgtttt cgctaattct
     4201 gtacctgcaa atattttata gatgggacca tcaagcttct tcttctctgc atcatattct
     4261 tgtaatacga taccgctgga tttattcggt gtgtccagac gataatccca tagttcattg
     4321 acaagccatt tttttcccgt ttcaagatca tggaacaaag aaggatcaaa gccgttacta
     4381 tttaaataaa ttggttcgga ccaaggacct gtgatctcag atgcagtgat caaataattg
     4441 tggacatctt taaacggacg gcctgcactt ttgacatctg tgtaaacaag ataaaacaat
     4501 ccatcagcat aactgatttg tggcgcccat atacttccat ttgtaggatt tccttgcaaa
     4561 gatacttgag tggtcaaaat atccgttgta tgtgaccagt tgactaaatt tttcgactca
     4621 tatactcgga ttcctggaag ccattcaaaa gaggagacaa taatgtaata ccgctctttc
     4681 actcttaata tattaggatc tggattgaat cctcttaaaa taggattttt cactttgata
     4741 gtctgcatga ttcaggctcc ttttctcatt cttactcgat ataaactgaa tgccccagct
     4801 gcgaaataaa aaaatgcaaa aggaaacgca ataaactgag tgccaatcaa caatatgaat
     4861 cctgttataa aatacaaacc agtggcagtt gttaagtatt ttcctttagc agtcaaaaac
     4921 aatgctactg ctaaaaaagc aagtgaaata aacaacacaa cggcaaacag accagtcatg
     4981 tcctgtaata atccaaatgt attttctaaa gacaaagttg aatgatctac agcaagcgac
     5041 tgataacttt ttttaaactc tgacgcagac atattactta aagtaactgc taacccgcca
     5101 aagaaaatca aagatagact gccaccgacc attcctagca atagttccca ttttcttttc
     5161 atttctgaac cccctgcttt tctaaatgta tccattcagg tcttttgatc gatgtttcat
     5221 aattgttatt acgttcagag aatttgacag tctgttcatt tttcttgtca tcccaattat
     5281 cccaaccagt tgggtgaaca tgcccaccta tcttacacgc ttcaaataag acatttgcat
     5341 acggacgcca cggtcttcct aaatagacgt ttctagtatc aggttctgca gtcaaggtac
     5401 agctttcaaa acgataccct gttttttgat ttttcggcgt agatgctgct gtgatgtatc
     5461 caggattttt actggctaag ctatggatat gacactgatg aaaaacggca tctgccccac
     5521 caaatatgaa atccaccgtt ccttcaatcc agcaatattc ataggtctga cgacaatact
     5581 caggattggc agaagccggt gtacaaaaag gagtaccatc tttttgcaaa tcaggcaacg
     5641 gacccgtgca taaggtatcc tgataaccag atagtcgaca gttttttaaa gatacgcgat
     5701 ctcctgaatt gaataaagca acagcctgtc ctaccttttc tcctggacct gctgtatttt
     5761 cgatgtgaag attttctaat acgacattcg atccttctaa gaaaaaagta gcacttcgga
     5821 aagtccctaa ctggctaccg tcaagatact cctgttttgc aaaacgatta ccggagataa
     5881 ctactgttcc tgctcccaca agtttaaaat tggataatct gctttctata tattcatcat
     5941 atcttccttc ttttactatc aagacctttg gatggctatc cggcaggcct gccaaataat
     6001 cgattccttc ttggatagtt tcaaaatccg tcggggtatc tatagcattc tttccaatta
     6061 tcagtttgtc tttcatcctc ataaatgctc ccttatgaat tgtttccaaa tatttctcat
     6121 ttcttgtgtt tccatatgac cgcctgttaa agaaaaagaa gaaaagtttt ctggactacc
     6181 cattgattga tactttttgt ccagcccttt tctcagcttc agtacgcctt cgtccaagca
     6241 catcgtatcg ttttttccaa ctaagctaag acgagggcgc ggagcgattt tttcttggat
     6301 tgctaacgtt gtataagctg tcaatagatt tggcacatag taataaaacc catgatgatc
     6361 taggcggcga tgtagcaaaa gtatttcgat gtccacctgt gccgctagat caatacaaac
     6421 tttgacacga gaatccaatg ctgtcaccca ccagcttaat aagcctccca tagaaagacc
     6481 aatcgttgcg agccgttgag tatctgtgtc ttcccttgtc tccaaataat cgatcaagct
     6541 gatcacatca taaatacgca ttccccataa cgtttttcct gttaataaaa attgtttgaa
     6601 caactcgctt tcagaaatac ctccgcgttc ttcaaatccc caagcatcaa tcgaccaaac
     6661 agcatatccc attcctgtca gtacttctgc aaaagatgga gaagctaagt aaggagcacc
     6721 atggatcaat tcacttttcc cgactgaaaa atctccgccg tgagaatgca ggtaaaggac
     6781 cggcggaaac ggtccttctt tttctggata agcatagagg gctggtactg tttccaattc
     6841 atttaagtac aattgatagt attcaagcat aaaaccgttt tgtttttctc ggtataatcg
     6901 ttttccacta ggggtatgtc tgctagatgg agcacctaaa aaatcattca actgcataag
     6961 caaagttttt acgaacaacc agttcatcca tattttctga ttgatttgat tggaatttct
     7021 caatactgat ttctttagta ttttctaata gaaatgctgg tccctcgtga ttttcaatcg
     7081 acatctggtt aaaggaaacc ttacccgcaa agccaatata aaatccttgt ctgctcattt
     7141 cctctattcc tgtcatcata gcaggaacac cgggaactgc atcttgggcc atcgagacat
     7201 cgatattgtc aaatgtgatt tcttgtatga attgttccgc taatccataa ataaaacctg
     7261 cagaagcatg gacattcctt gccgtgatat tcgaaaaatg gatccttctg aatgctggtg
     7321 tccgttcatc tatcggataa gccttttttt cccatacata cggttctttc cctcgcggac
     7381 cacagaaata ataaagattc agtataaatg gacacatcac attatccatg actatattgg
     7441 atactcgaat atcttcaacg atcccgcctc gacctcgtct agatttcaat cgtattcctc
     7501 gatcagtttc ttggaagata caatttgaaa tggtaatatt acgaatacta ccactcattt
     7561 cacttcctaa tacgacaccg ccatgtccat ggaccatcgt gcaatttgtg atcgtgatat
     7621 tttcgcaagc gatcctttcg tacgtatctt cagtccctgc cttgattgca atacagtcat
     7681 ctcccacgtc aatatggcaa ttactaatac gcacattttt gcatgattct ggatcaattc
     7741 cgtctgtatt tggtgaatcc gctgggttca agatcgtcaa gttatcaaac gttgcattac
     7801 tgcaaagaat cgggttgatc gtccaacttg gtgactggat caatttaata tctttcacag
     7861 taattcgatg acagttatgg aaactcatca atttaggacg aggataagcg agattatctg
     7921 gttcatttct aaaagtatgc caccattttt ttccatttcc atctaatgta ccgaaaccag
     7981 tgacagagat gttctctaca ttttgagcat agatacagga agcataaact tttcgatgga
     8041 ctccttccca acgtgaatga acaacaggat aatcttttgg atcgtcagag aatttcagaa
     8101 ccgctcctgc gcttaaatga agttcgacat tgctttttaa aaataaagct cctgttagaa
     8161 attcacctgc tggaacaact acggtcccgc ctccgtcact tgctgcagca tcaatggctt
     8221 gttgtattgc ttcagtattc aattcatcaa ttgatgcgcc aaatgtcaaa atatcataca
     8281 tatttataat gcctccactt caatcaatgc ttgcaagaat gcaccaacgc ctttttgatc
     8341 attacaaata attggttcac tgatgtagta agcatatgaa ccatctcgct gatctgctcc
     8401 gcctaaccct gctacttgac agtttttatt caagttcagc cagccttctt ttgtttccag
     8461 aacaaattca tcaagtaatc cttgatgtga tttcttcact tgtggaagcc attcttcttt
     8521 gttcagcagt cccaaacgta ttcctttagc cattgcgtaa acaaacatac tactgccgga
     8581 agcttccaga taatttcctt tccgttttgt ttctgttgtt acttgatacc atgtcccgct
     8641 gttttcttct tgatatggaa gtaatgcagc cagtgtcttt tctagaattt ctttcagcaa
     8701 agaacaatct gtttcttcat gaagtaattc ataggtatct gctgcagcca tcaaatacca
     8761 gccaatcgat cggctccaaa aatgatggga tagacctgtt tttggatcag cccatggttg
     8821 gaccttcttt tcatcccatg catgaatcag caaatttgtt tttggatcga atagatgttt
     8881 ataactgatt tcaaactgtc tcaaaacatc ttggtaatct ttttcttcac taaaattctg
     8941 gataaattca gcataaaaag gagaacccat atataagcca tccaaccaaa tttgataagg
     9001 ataaatttct ttatgccaaa aagctccttc ggatgtgcgt ggatggtttg ccaactgctg
     9061 ggaaaggagt tctgctgctt ttttatattt ttcttcgcct gtttctttgt acaataaaaa
     9121 gaaaagcttt cctgtattta catggtcgat attgtattct tcaacgctgt agccacggat
     9181 cgttccatct tcttgcacga aatagtcgat attgtctttt atgaaatcaa aataaatttt
     9241 ttcttgggtt cgttcccaaa gtaattggaa ccctttaagt acgacgccat agtcataaga
     9301 ccattttcct ttataccact tttcttcata taagcgaggt gttctttcca tgatactttt
     9361 tgctaatttc tttgaccagt tttccattat ttgctctcct ttaaaaatta aaataggatt
     9421 tgggacaaat accaagaagt atttgtccca atcgtctata ttactgtgcc gctgctttgt
     9481 atgcttcctc gtactcttgt gtgattcgat cgcctccttg tgctttccag tcttctacag
     9541 cttttttgaa tccatcttcg tcaagctgtc ccatgatgta ttgcaccgta gcatcaatga
     9601 cgattttttc aatttctgat cctacttcat tgttcgtcgg tgaatccaat ggcacagaag
     9661 gatccattac agcaaattct tcattttctt tgatcaattg attagagaat tctttttcag
     9721 ggtctgtatc tttgaaagag aacgtcactt cactaggacg agagcttgat aacggttgca
     9781 cctcttgttg ccaaagatcc atatcgataa attcgataga accatcatca ttcaattcat
     9841 aatgcgttcc ttcgataccg ccagtcatca atttatacac atcttcttct aaaagatcat
     9901 caacaaattg aagtaatttc ttcaattgtg cttcgtcttt tacttgagat ttgggaaatg
     9961 ctagcaagcc gccaatacca tttccttctg accaaacatg ataaccgccg ccttcaccat
    10021 ctgagatctt gtttacaggc actaattcca tatcatcttg aataccttga cccatcgtct
    10081 tcagattctt gatatccacc attccagtgt agattccagt tcttccttgt gcaaattgtt
    10141 cttgctgatc agtcttttgg gtaactgcaa agtcttgtgc caaatagcca ttctcaaata
    10201 gatctcttga aaaattcaaa gctttcatat actcttctgt atcaaattct ggagtgaatt
    10261 tgccattatc gtctacagcc cagccatttg gtgctccaaa ataagaagtg aaaagtttaa
    10321 agctggtata acgaatatca ctaggcgcac gatcgccaaa accaactgtg tcgtccacac
    10381 catttccatc aggatcgttt tctgtaaatt gtttagctac ttcataaagc tcatccatgg
    10441 tagttggaac atctagtcct agattatcca accagtcttt tcgaatgact aagccagcac
    10501 gagcgtagtc tttttgtatc ggtacgccgt acaaggcacc gtcgatactc gcagcttcga
    10561 ttctatcttc agaaatttga cttaaatttt tatagtcttt taaatattct gatacatccc
    10621 agaatacgcc tgattttaac gcattgcgga cagaagagtt tttcaacatc gcaatttgaa
    10681 gtgtaacgat atctcctagt tcatcagaag ccaatgcagt tgttaatctt tcttcttttg
    10741 aagcatcagg aatccagttg aatgtcagtt ccgtatcggt tttttcttcg atcaagttca
    10801 caatcgtgtc tgtaggcggt gaagcatgat gcaaaatatt taaccaagtg attttagctt
    10861 ttccatcttc tgtctctgaa gaactgttac ttgaactgct gccgcatgcg gcaagtgcta
    10921 gaattcctgc aaaactaaga cctaatttta cgagcgttgc ttttttcata aattttctcc
    10981 ttcactgttt taataatatt tttgttttct tctgtttttt ctgtatatga gttcttccat
    11041 tccaaatggc aaaatataga atttgctgtc cacgtgactg cggaaaagaa gagactgcca
    11101 ccgataaagg ttaaaacacc tggcattgtt tggatgatca tcagataaat ccctaccata
    11161 cttaatatca acactaatga gtaatgaaga tgtgagaatc cgaataaaaa tgccattttc
    11221 attttgaaaa aatatccttt ccaatcataa tgagccatga ttgggaaaat atagagtgct
    11281 gagagcacat atatgaacgc agcaatatac aaagcgatcc taagatacca attgtgtatg
    11341 aaagaaagat ccacaagtaa aacaatcccg atggcgccat atatccagct catcagcaag
    11401 gtttcacgat agttttcttt aaaatatgtg aagtacgttt tcgtcaaagg aaacgtttct
    11461 tttgagcgca gccattttct aatcactgca accatcgcat acgatgcagg accgaaagta
    11521 aaaactccca aacctgcgat gactaaaccg ccccacagaa aattcaaata aaccaactgt
    11581 aaaatacgat ttagccttcc gtttatttta tctaatgact gcagcatatc tttccctctc
    11641 tttctatctt agtaaacgcc gtcttctcct agctttttgg caatcttatt agctaaaatt
    11701 accataaaca atcctacgac ccctttaaac aatcccattg ctgtactgaa acttaactgt
    11761 ccattcttca atccagctgt ataaatatag gtgtcgaaaa tttctgcaac gcttcgattc
    11821 aatgaattca ataacaagta catatgctcg aaccctaagt ctaatgtctg accgattttt
    11881 agaatcagca aggtaatgat tactggacga attgccggca atgtaacatg ccatgttttg
    11941 cgtaagcgtc ctgctccatc catttctgca gcttcataca actgtgtatc gactgcagtt
    12001 atcgctgaaa ggtagatgat cgtagaccag cccaactctt tccagatcac ttgtccgata
    12061 tatactgtgc gtgtccattc aggggatgtt agaaaactga ttttttcaaa tcccattgtt
    12121 gcaaggaatt cattcaatac ccctccgtca acattcaaaa atacataagt aatcgaaaca
    12181 atgattaccc aagacatgaa gtgagggatg taaatgatcg tttgaatccc gtttttcaat
    12241 cgtttgttcg ttacttcatt taatagtaaa gacaaaatga ttggcatcgg aaaaaacacg
    12301 aagatattta atccgaacaa gatcaacgta tttcttaata atgtaaagaa tgtcggttct
    12361 gtaaacaaac gaatgaaatg tttgaaaccg acccaaggac ttcccattat tcctaaaaat
    12421 ggttgataat cttgaaaagc aataacgagt ccccccatag gaagatacct aaaaatcaag
    12481 aaataaagga ctcccggcag aatcatcaca tataaaaatt tattcgtttt gattcctgca
    12541 attatttttt ttcgtcttct gagcttttct tcttttttcg atagcgtcag ttgaaatggt
    12601 tcttgtttct ttacactttc cataagcccc tccttagttt tgtgaatgtt tgctgcttat
    12661 gacttcatga tagataacta tctgaattac gtatataatc atttttaccg taagtaaaaa
    12721 cgcttacata gcaatgattt ttgtgctata caaaacgata aagtgtattt tttcacaaat
    12781 aaactacgca tagcaaacct tctatttttt tctatctgac acttagcata ggaaatgatt
    12841 atatacgtaa tctttcaccg atgataaatt gaacatatag aaacagaaac atttaattta
    12901 ttttgaggag gagcctatct atatgaaaaa gaaagtctct ataggagaaa gaagttttac
    12961 acttttcaac aatacgtttc taattttgct agctttgatc tgtatcgttc cttttttaaa
    13021 tatcatcgct acatcttttg cttctacaca agaagtcgtc gcgaaaaagt ttatcctttt
    13081 tcctactacc ttttctttag acgcgtatcg ctacattctt tctacgccga ccatttttag
    13141 agcacttgct gtttcgattg gtgttacagg tgtcggaacg atcgtcagta tgtgtgcaac
    13201 ttcactgatg gcctacggtc tttccagaaa gtatttgttt ggtcgaggtt tcgtcaactt
    13261 tcttgttgta ttttctatgt tgttcagcgg ggggatgatt ccaacgttct tagttgttcg
    13321 ttctctaggg ctggtcaatt cttactgggc catgatcctg cctgtggctg ttaatgcaat
    13381 gaatatgatc attatgagaa actttttcca agccttgccg gatagtttgg aagaatcagc
    13441 aaaaatggat ggttgcacag attttggtgt atttttcaaa attatgctgc cattagcttt
    13501 accgtctatt gctactatct cgctttttta tgctgttact tactggaaca cttatatgac
    13561 tgctattttg tatatcaacg attcatctaa atggccgata cagattcttt tacgccaaat
    13621 cgtcattgta tcaagtggga tgcaagcaga aagtagtgcc gttgatgtga ttccaccagc
    13681 acaaacagtt aaaatggctg ttatcgttat tgcaacagtg cctatgctga ttgtttatcc
    13741 ttttgttcag aaatattttg tcaaaggagc actcgtaggt tctgtcaaag ggtgattatg
    13801 tactgaacgc aaaatggtag ttgtgtcaga agtggtcagc ttcaagaaat aagaaggcat
    13861 ttacgaaaat tgcttcttaa atttttgtga aatttcggct tatttttcga agaatctact
    13921 cctggaacac cgtgtatcag aggttgtgaa agagacgttc agccttgagc gatgataaaa
    13981 actctcacaa acattgcgac gagtaaccca caggagcaat aagaagcgat tttgaaaaat
    14041 agcttcccat atttcacaaa attatgcctt attgtcgata gcctgtctct tgaacgccat
    14101 ttattcgtaa aaaagaactg tgacaagact tttgtcacag ttctttttgc gtgcttagcc
    14161 ttgaaattta actatccata aatacaaata ctagggttat tctaacttct tttattgctt
    14221 gaatcagtcc acaagaaaaa cagcctcttg attaaacggc gtttatcttg ttgattctgc
    14281 ggcaataaaa tcagccagca gcaaaacatg aagtgctgtt ttttgctgtc ctcttttatt
    14341 gcttgaatca gatcacaaga aaaacagcct cttgattcac aaatattttt ttcgatattc
    14401 tcccggcgta atcgattcct tctttttaaa gaaacgaata aaattttgcg gatttcgata
    14461 ttgcaatctt tctgcaatct cttttacgga gaggttagtc tctttcaacc atttttttgc
    14521 tacctctaaa cgatgatttt gaacaaaatc accaaatttt tctcctgttt cctttttaaa
    14581 tacattactt aaatagttag ggttataatg caacctatca gcgatcacat ctaaagacag
    14641 atcacgatca tattctgtgt agacgatatg cataatattc tccgacaaat ttttaaattc
    14701 atgatcagtg attgcttgag agcattctgt gattggcaga actaattgtt ctaaaacaag
    14761 tttttctagt tctttgggat gataattgtt cagtgctttt acataaattt gtttcatgtt
    14821 atcaaagttt tttgaatcta ctcccaataa ctgtcctaat tgaacgagtt cattgatcaa
    14881 gcggaccgaa actacttccc gactcaaagg atttttattt tgtgcaaaca atgcatcaat
    14941 caattcatgt actaaattct tcacttcctg atttccagaa cgaatggctt caaacaatcg
    15001 attttgttct tctatcggat atctaataag tgttttttca tgagcagcag gaacgatttc
    15061 ctgataaaaa ataattgaac ttggaccagt atttacacga taatacaggg cttcttttgc
    15121 ccgatcaacc gattgcttgc tttcattcag cgtttcgaac cggttactga tgcctatact
    15181 gatagttaaa ttcaagtatt cttttacacg tttttgaatg agtcggcaat actccatgac
    15241 ttttttgctt gcttctgcat catctttatc cattcgataa atagtggctt gcatttcttc
    15301 attcaaaacg atcggcagca ttcgattttc ctttggcaca atttccgtga tcatgttgtt
    15361 gatcgctaaa agtagcaaat cacgctcccc accgtgacta tcttctaaca gatcgatttg
    15421 aaccaaagct gtataatagc agtcattttg agaactataa ccaaattgct gcaaccgctg
    15481 atctagttct cgcctttcta ctcgatttct aaataaacta agtacaaata gcgtttccag
    15541 ctgtggcttt tgggtaacta agtgtgcact taacgcttct ttttctccta tgatccgact
    15601 gactgattga gcgaccaaat ctagttcttt cccactgaat ccttttgatt taagattcag
    15661 acgttcctgt atttgagaaa tcggacgggc aaatctgtcc gaaaataagt aggaaaaaag
    15721 accaatgagt cctaccagtg ttaaagacac tgcaactagc ccgattctca gattgcggat
    15781 cattgcgcca attgcactag agtcaacttc taatctgtaa atccaattat tataatctga
    15841 tttcagtact atatacgttt tatctttttc tcttatcact ttcagctgat tgtaatctag
    15901 atccacgtcc gttaatacag ttggttgctc tttatttttt actgtctgag tagtaaaaag
    15961 gttgccaact tcgttttcaa tagaaagtat gtcttctcca ctttcaccaa caacttgttc
    16021 tattgtatgt ttctctatcg tagcaattcc caatgcatat ttttcttttt caaacattgg
    16081 caaagaaatc accatttcga ttcctttgtt tttcttttgc caaaataaat tatttttgtt
    16141 atttatgtac ttctgttgat acttttcaac ttcctcttcg gtcagttgac tcaacgatcc
    16201 attgattact ccccatttac cagccaaact gactaaatca taattattat catccataac
    16261 aatcgtttct ataaaattta gttctttcct gatttcagaa taagtggagt aatctgtata
    16321 tgtcagcgga tgcttgaata tgtccgagaa atttttggtc gaaccatacg tagaaaacga
    16381 atattcaatc gttctaattt tccattcaac gttagtttta acttgcctga gcaaattctt
    16441 tttgtctgtt aaataactcg attcgatcga ttcataggta ttctggtaaa tgaataaact
    16501 aaatcctatg acaagtacgg ctcctaataa aaacatcggt aaaaatattt tataaaatac
    16561 tgtacttttc ttcattttct tcctctcctc cttttaaaat aaaaggcttt catttttctg
    16621 taaaattgat actcatctaa ttaaggctct tcgtcaaatt gtgttggcga agacaaattt
    16681 gaataaaact tagtccgata tgcggcgcgt tctgtgcgcc gcatattttt ttagttttca
    16741 aagatcagac cttgttgtaa atagatccgt gcccgaaagt tataaaagtt gcgataacca
    16801 aacgcaattc gtttaatcag cttgatcttg ttattcagcc cctcagtgac gccgtttgag
    16861 tggggcacct taaaggcatt tgaaataccc gattggtagc gatggaagat cgtgaatttc
    16921 tttttaaacg actgaggtaa cgtagatgaa attgttttaa gggtttcttg aaagccttcc
    16981 gta
//
LOCUS       DAYYGO010000062        16954 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000062 DAYYGO010000000
VERSION     DAYYGO010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.063"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(277..2169)
                     /locus_tag="ACHA5T_001706"
     CDS             complement(277..2169)
                     /locus_tag="ACHA5T_001706"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8887277.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2166..3893)
                     /locus_tag="ACHA5T_001707"
     CDS             complement(2166..3893)
                     /locus_tag="ACHA5T_001707"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8887278.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3909..4559)
                     /locus_tag="ACHA5T_001708"
     CDS             complement(3909..4559)
                     /locus_tag="ACHA5T_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFM8887279.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4763..5059
                     /locus_tag="ACHA5T_001709"
     CDS             4763..5059
                     /locus_tag="ACHA5T_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM8887280.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5231..5851
                     /locus_tag="ACHA5T_001710"
     CDS             5231..5851
                     /locus_tag="ACHA5T_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFM8887281.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5943..7109)
                     /locus_tag="ACHA5T_001711"
     CDS             complement(5943..7109)
                     /locus_tag="ACHA5T_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8887282.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7121..7474)
                     /locus_tag="ACHA5T_001712"
     CDS             complement(7121..7474)
                     /locus_tag="ACHA5T_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFM8887283.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7602..8624
                     /locus_tag="ACHA5T_001713"
     CDS             7602..8624
                     /locus_tag="ACHA5T_001713"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFM8887284.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8643..8804
                     /locus_tag="ACHA5T_001714"
     CDS             8643..8804
                     /locus_tag="ACHA5T_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887285.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(9063..9497)
                     /locus_tag="ACHA5T_001715"
     CDS             complement(9063..9497)
                     /locus_tag="ACHA5T_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887286.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9607..11466)
                     /locus_tag="ACHA5T_001716"
     CDS             complement(9607..11466)
                     /locus_tag="ACHA5T_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8887287.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            11467..11607
                     /locus_tag="ACHA5T_001717"
     CDS             11467..11607
                     /locus_tag="ACHA5T_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFM8887288.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(11584..12744)
                     /locus_tag="ACHA5T_001718"
     CDS             complement(11584..12744)
                     /locus_tag="ACHA5T_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8887289.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(13051..13962)
                     /locus_tag="ACHA5T_001719"
                     /pseudo
     CDS             complement(13051..13962)
                     /locus_tag="ACHA5T_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
     gene            complement(14128..15321)
                     /locus_tag="ACHA5T_001720"
     CDS             complement(14128..15321)
                     /locus_tag="ACHA5T_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8887290.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            complement(15405..15605)
                     /locus_tag="ACHA5T_001721"
     CDS             complement(15405..15605)
                     /locus_tag="ACHA5T_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFM8887291.1"
                     /translation="MNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVIM
                     NGNRIQIKRKQFEKIIDTLDTI"
     gene            complement(16029..16262)
                     /locus_tag="ACHA5T_001722"
     CDS             complement(16029..16262)
                     /locus_tag="ACHA5T_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8887292.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(16269..16700)
                     /locus_tag="ACHA5T_001723"
     CDS             complement(16269..16700)
                     /locus_tag="ACHA5T_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8887293.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
BASE COUNT         4766 a         3705 c         3181 g         5302 t
ORIGIN      
        1 aagaacagcc aaacaaaaat agctcctcct atttttcgtt tggttgggct tattaccgca
       61 gatgcaagct tttgaacacc gtttaaggaa gaggttgtaa aatcagcggt ttgacctgag
      121 tattagagat acaaaacgga aaaatagctt ctcatatttt gccgtttttt gagctaatac
      181 cgaaggacag ctgattgaac accgtttatt cggaaaaagg gactgtgaca cgacttttgt
      241 cacagcccct aaaaaaaata ataaatttgt actattctat gaagcttctt cttcagaaaa
      301 ctgactgtta taaagatctg catagaatcc gtctttttcc atcaatgtat cgtggtttcc
      361 tgtttctacg atcgaaccat gattcatcac aataatgttg tctgcatcgc gtattgtaga
      421 caagcggtga gcaacaacga aacttgtccg gttttccaat aggcggttca tcgctttttg
      481 aatcagtatt tctgttcgtg tatctacact agatgttgct tcatcaagga tcaggacatc
      541 tggatttgct aagaatgccc gcgcaattgt gattagctga cgctgccctt gcgaaatatt
      601 gcttgcatcc tcgtttaata cagtgttata tccttctggc aatttacgaa caaaatcgtc
      661 tacgtgagca gctttagcag cctcaatgat ttgttcgtct gttgcatcgt cgttcccata
      721 tttgatattg tcatagatac ttcctgtaaa cagccaagta tcttgcagta ccattgaaaa
      781 ctgtgcgcgc aattcatcac gtgaaagatc acgggtatct acaccatcgt aacgaatact
      841 acctccagaa acatcgtaga accgttcaag aaggttgatc agtgtcgtct tccctgcacc
      901 agtcggacct acaatagcga ccatctcacc aggtttgaca tccaagttaa aatcagtcat
      961 tagcaagtta tctggtgtgt agccgaattg aacattttca aaacgaactt tgtatggtgt
     1021 atctttttct tctggaaggc cagatttttc attggacatc tcttcttcat ccaacacttc
     1081 aaatacccgt tcggctgaag caactgttga ttggatagtg ttcatcaaac ttgcaagttg
     1141 agtgattggt tgagagaact gtgttgtata ttgcaagaac gcttgaacat cccctaatgt
     1201 catgttccca tttgcaactt ttactccgcc tagtacagcg acgaacacat agcctagatt
     1261 tttaacgaaa ttcatcaacg gcatgatgat tgccgaaata aattgagctt tccaaccagc
     1321 ggcatacaga ttctgatttt ctttttcaaa tacttcctgg tctttttcag tatgattgaa
     1381 tgttttaaca atggtatgac caccatatgt ttcttctact tggttattca ataaacctaa
     1441 acttttttgc tgatctgcga aaaacacttg tgatttcggc gctacgatca tcacgatgat
     1501 caagcttaaa gggatcatca ataacgcgac taacgtcaat tgccagctga ttgaaagcat
     1561 catcacgatg accccaatca atgtgactgc actagttaca aattgagtca aattttgctg
     1621 cagcgtgctt gcgatgttat ccatatcatt gattgctcga gacatgatat ccccatttgt
     1681 atgtgtatca taataggaaa tcggtacacg attcattttg gcttccaaat catgacgtaa
     1741 ttcataaact gttcgttggg aaacacgtgt cataataaat tgctgtaaaa agttgaatac
     1801 agcagaaatc aaatacatca cgattactgt cgcaatgatc tgcgcgattt tatcaaagtc
     1861 tatcggaaat tttgttactt gttggccttg ttgcatctgt tgagcgccag ccattacacc
     1921 tttgaatatt tctgtggttg ctttccccaa gacctttggt gtttggattt ggaagatcac
     1981 ggctgctatt gctagtacaa atacagcgat gatcgcaacc atacgtttag acatatagcc
     2041 aaacaatcgg cgtaccgttc cccagaaatt tttaggcttt tcacctttta caccgagatt
     2101 tctgccaggt ccaccatgtc ctggaccgcc tgctggtcca tgagagggac gtgtgtttgt
     2161 tttactcatg ctaagtcctc ctctcttagt tgtgaatgaa cgatttcttg ataagtttcg
     2221 tttgttgcta atagttcttc atgtgttcct ttgccaacca gttttccttc atctagcaca
     2281 aggatcatat ctgcatccat tactgtactt actcgttgag caactaaaac ggtgatactt
     2341 tctttcatat tcgttttcaa tgcttggcga agattggcat ccgttttgaa atccaacgct
     2401 gagaaggaat catcaaacac atagatatct gctgttttga ctaatgcccg tgcaattgcc
     2461 agacgctgtc tttgtccacc agagaagttt cctcctcctt gttccacatg gctatctaag
     2521 ccatcttcca tctctgaaac aaaatcttta gcttgtgcga tttctaatgc tttccaaatc
     2581 atttcatctg ttgcatccgg cttaccatac tgcatattgt ctcggatcgt tccagagaac
     2641 agaactgctt tttgaggaac aaagcccatt aattctcgca aattatattg agtcatgtca
     2701 ctaatgtccg ttccatttat ttggatcgaa ccagattcga tgtcatataa tcgagggatc
     2761 aaattgacta aggtcgtttt acctgaacca gtccccccta tgattgcgac gatctcgccc
     2821 gacttggctt ggaaatcaat atcttccaat gcaagttttt ctgctccatg atatcgataa
     2881 ttgacatgat tgaaggccaa tgttgcgttt tttccagcaa aagaaactgt tttaggattt
     2941 tcagggtcat ggataccgat tttttcatcc aacacttcat taatacggtc agctgaagct
     3001 tgtgcacgag gcaccatgat aaaaatagcg gaaagcatca taaagctgat caagatctgc
     3061 atcgcataag tcataaatgc aatcagatta ccgacttcca aagtcatatc tgctatgtag
     3121 tgtccaccaa accaagtgat tgctacgttt gtaccgctca tgatcaatgt catcattggc
     3181 atcatcaatg cgacgatcgt attaactttg attgccgtat ttgtataatc tttattcgct
     3241 aaatcaaaac gattttcttc aaagcgtgtc ttatcgaatg ctcgaatcac tctgacccct
     3301 gtcaatcctt ctcgaaatac caggtttaaa cggtcagttt tcttttgcat acttttgaat
     3361 aatggaacag caaagtacat gattccgccg atcaaaataa tcattactgg gatgacaact
     3421 aagaagattt ttgtcaactg atgatctttt tgataagcaa gtatgctcgc gccaatcaac
     3481 gtgattggtg cattgatcat catacgtaaa aacatttgtg taaccatttg gatttgagtc
     3541 acatcattgg tcgttcttgt gatcaaagag gccgttccat atttatcaaa cgcatctttt
     3601 gtcagactct ctgatttttt atagacatcc gtacgcaatt gcttacccag tttctgtgat
     3661 tcacgagtag caaaaaatgt atttgcgata gcagctaaga tactgatcaa agaaaagccg
     3721 atcatcacaa aacctgtatg ccaaatataa tcgacatctc cttgcgctac ccctttatca
     3781 ataatattgg aggtaagtgt cggcaaatat aaatctgcca gtacttggat caccatgaaa
     3841 actgcagctg caattgctga agtcaaggag atccgtttta cgatttttag catctagttc
     3901 ccttctttct atttatatac tccgtttttc agccaagaaa gtttcaaatt gaattcactg
     3961 attgcttgct ctactgagta atcttcagat gttgaaaata agcgatacga atgcgcaatt
     4021 gttgtaaata cagtattcat cactaattgg atcagtaatt ccaattcatg atcatttttt
     4081 actttcagct tcgtccgatc gatcaattca agcaactctt ttgcatcttg tttgtgttct
     4141 ttatgtggat cgtgaggcgt tttttggcga ctcgtatgag gagcgacttt tcttgaggca
     4201 cggtaatcca tgttcataaa caagttacga taaaaagaag cattctcgcc ttccaaaatc
     4261 tcataaatca ttttagaaaa ataggattcg aatcctttga atagatcacc atcggattct
     4321 ttcattagtt gtaacatatc tttagagctg tcacgtctca ttgtctcaaa gtaataaaaa
     4381 taaagatcct ctttatcttc aaaatactga tagaaactgc ctctagggat atccgctaat
     4441 ttgacgatat tagcaatcga agcttctttt aagggtactc tagaaaactc gattctcgca
     4501 gcctcgatta gtcttttttg tttttcttta ggcaaatgga aaaatgtctg tgtcggcata
     4561 tggatcctcc tttctttcaa gatgacacat tgtcatatga cgctgtgtca ttataacacc
     4621 tcctacctgt tttgcaagac atttgcatag tttttttcat tatcatccga tacattaaaa
     4681 aagtgataag aggaatcctt ccatttgaat taatgagagt tgcccattaa aatagccaca
     4741 tactaaacaa aggatgagat aaatggccca atcaattagt gtccaagact tcaataaatt
     4801 gcctctcgat gctgacagca tcgttttaga tacaagaaat accttagatt acacaaaaaa
     4861 ccacttaagc cactcgcttt ctatccccgc tgctatcttg ccaagacaat taaaaaatct
     4921 agatccaaag aaactttatt atattctaag ctatacaggc aggcgatcag aagcattagc
     4981 cgatcaactt actgctaatg gttttcgtgc tgtttatatc attggtggga tggaagctct
     5041 tcagtcgagt gttgcttgaa tattgcttgt tagattcaaa agaggctgtg acaaaaaatc
     5101 gtgtcacagc ctcttttata ccctgtcttt ttttagttat ttattcttca accttatcaa
     5161 tatgacttga tagccttttc tttttctcat agttataaca atcatagcaa taaaaggaga
     5221 aggtaagcat atggcatctg ttttagtagt caaaggtcat cccctaactg cagaagaatc
     5281 gcgcacagtt aaggcattga cgtctttttt aacaagttat aaagaaaatc atccagatga
     5341 tgaagtgact gttttggaac tttatcgcga tgatattccg gaaatcgatg aagaattatt
     5401 atcaggttgg gaagcattac gtgctggtgc agaattcact tccttatcag aaagtcaaca
     5461 acaaaaaatt gctcgtttca atgaattgac tgatcagttc cttgctgctg ataaaatcgt
     5521 tatcgcaaac gccttatgga atctaaatat cccaacaaaa ttaaaagctt ggttcgatac
     5581 cgtcaatgta gctggaaaaa cgttccgcta tacagaaaac gggccagaag gattagtgac
     5641 aggtaaaaaa gcactgcaca tccaatctaa tggcggtgta tacaacggac aagactttgc
     5701 ctctcaatat gtaaaaggta ttttaaactt tgtaggcatc gatcaagtag atcagctatt
     5761 catcgaagga atcgattatg accctgaccg tgcagatgag ttgatgcaaa atgcattaga
     5821 taaagctgct gctttaggta aatcatttta aaaattgaga taaatgcata caaaagagac
     5881 agaggaaaaa atcttcaaag cagcatttgt accttggata ttttcccatc tgtctctttt
     5941 tgttattctt tctgcttttt caacggattt tcttcataaa gagaagagat tgttgtgtac
     6001 cattcaaaaa catgagtaat cactacttta gctgctgcat aaacaggaat ccccaagatc
     6061 acaccgatga ttccgaacaa tttcccagaa gtcagcagaa ctaataaaat cgttactgga
     6121 tggattgcta actgattccc tagtactaat ggagagatca agcgtccttc tattgtctgc
     6181 tctactgcaa aaacgatcag tactttgatg atcatgaatg gacctgctac aatggctaaa
     6241 aagatagccg gaatcatcgc taaaaatgat cctaagtaag ggatcaggtt caaaaaaccc
     6301 gcaataatcc ccaacgtgac agcatactct aaaccaatga ccgcaaaacc aatcataaac
     6361 atgattgcca cagcaaaagc cacagttagt tgtcctctta tataagaaga aacttgacta
     6421 ttcatttcac tcagcacatt caacgtcggt ttttgcatgc gtgtcggtaa aaatttgaca
     6481 aaataaggtg ctaattgttt accgtctttc aatagataaa ataagataaa cggcatcgtc
     6541 agcagagcca ctaaaatagt cgctaccgcg ccgacaaaac ttccaagact ctgaactgta
     6601 ctcttagata tatcttggat catatcgcct gccgaattca tgaacttatc acctgctgtt
     6661 tccaactgtt cgcgaaattg gttgaacagc gggtcccgca aaatctcggt taatttatta
     6721 tcaatcgtat ctacatattg agggaagtta ctgatgaagc tcactgtttg ttcctgaatc
     6781 ttagggatga tcactacgct cccccatgcg attagtgcaa caaccaaaac aaacagccca
     6841 atgatactgt aaagccttgg tacttttcgc ttttcaaagt aatcaactac cggattcatc
     6901 aagtaataca agattcctgc taaaatgata ggcagaccta cgattgctaa aaattgccag
     6961 attggtgcaa acaaatatga aacttttgta aataaaaaga cgatcagcaa tattaacaat
     7021 acaacaagta aggctgtgac tacttgatta tttaaaaacc agcgccaaaa ccacgatacc
     7081 ggtctgttct tcttattttt tccttccata attcctcctg ttagttataa aaaatttggc
     7141 ttcccaaacc aatagcaggc agtctatgcg gatgaagata gattccattt gcaatttgtc
     7201 cttcttctgg acaagcatca aaaatcaatg atacgtgagc tactgtctct aaattcccat
     7261 tggccatcgg accgacataa tcgatcgtat atacctgatc atcaaaagca attgttccgc
     7321 ctgctttcaa agaaaaaggt tcgtcttttg atacttcttg aatcaccgaa tattttcgta
     7381 aggcagctgt tgctgtttta tcgaacaaaa tcagcatggg ttcttccttg tttattgctt
     7441 ctgtcccaat ttctgtaatc tttgcctgaa tcattgcact ctctccttta attgctttca
     7501 aaaacagtat aacacattct cattctttag ccgtgtggaa taattatgtg atagaataaa
     7561 aacgaaaact atttcattac aattaggagg aatcagctca tatgatcgaa aagataattt
     7621 taggaaccta tacgcgtcgc gaaagtaaag gaatctatac gatcgacctt gatacagaaa
     7681 aagaagaatt agccaacctt tcattagctg ttgaagaaaa tagccctacc tatgtagcaa
     7741 aaagtaaagc tggcaatctt tacacagtta ctagtgtgga tggattaggt ggtgccggtg
     7801 cgtacgataa ggattttcat tttttgaatg cggtaacaga gtctggcgct cctttgtgtt
     7861 atgtagcggt tgatgaagca cgtcaattac tatacggggc gaactatcat aaaggtgaag
     7921 taaatgtcta ccatatatta ggagatggcg aactgaaagc agcagattca atattccatc
     7981 aagaagcgac aggtcctcat aaaaaccaag atcatgcgca tgtccactat acggatctga
     8041 ctcctgacca acgattagtc gtatgtgatc ttggtacaga tcgtgtgtat acttatgatg
     8101 tgtccgaaaa cggcaaattg aatcttgtaa ctaccttcac tgcagagcca ggaacaggcc
     8161 ctcgccatct tgtctttcat ccaaatggac aattcgctta tttatttggt gaattagaca
     8221 gcacagtccg cgtgctttct tataataaag aaacaggtgc ttttgaagaa ttacagaaaa
     8281 tatcgacctt gccagaagaa ttcgacggtg aaaatggagg ggcagctatc cgcatttcaa
     8341 atgatggcaa gttcctttat gcttctaacc gaggacataa ctcgattgct gtattcgcag
     8401 tttttgaaga tggttcccac attcaaaaca agcaaatcat ctcaacagaa ggggattttc
     8461 cccgcgattt cgcactagat ccaagcaata attttgtcgt gtgtgccaat cagaacaccg
     8521 ataatctgac attatatcgc cgtcatcctg aaacaggatt actggaatta ctgaaaaaag
     8581 atgtctatgt accagaatgt gtctgtgttt attttgatcg ttagtaagaa aaggagaatg
     8641 aaatgaatca gcaagacgta ttagacagac tacgggaaga actaaatatt cctttctttg
     8701 atgggaaaat cgaagataaa gagtatagcg aagaggaata tcagaaactc aaagccgatt
     8761 tacagaacta ctttgaacaa tatgtacaga atgtagaaaa ctaatcagct ttccatacta
     8821 tctgaaaaga acatcaggac aagacttttc cgtctatcct gatgttcttt tctacaaata
     8881 aaaaaccgcc gagatggtta gactcgacgg aaaaggagta tttactcgat tttgagtaag
     8941 tacaaggttg ttgttggtta taggtatgtc attatcataa aggattttga tcactctatc
     9001 ataaaggtaa tattacattt atattagata actctagttt ttattgttat caaagatttc
     9061 ttttattttt tcaacaacaa attagtcatt ttttggatgg cagctgtcaa ttggtctgtc
     9121 gcttggtctt gttttgcttc agatgtattg aatagatctt catctacccc tgattcaaga
     9181 tcgatacttt ggatcatttc ttcacagcct gctacatatg aaagataagc tttttcaaaa
     9241 tttttatgaa tgcccattgc ttttgctggt ggacgcaaag tactgacttt cttaagcatt
     9301 gcccgatatt tttttgttcc ttcttcaaat aacaggatga tctcttcaag agtcgatttg
     9361 tccagttcac ttgtttttcc ttcatcaatt gcgtttctga tcacttcata tttcggatgc
     9421 attccttctc cgattgcttc tgtttccttg acgatttgat cgatggactg cacataatag
     9481 ccaatattcg gtaacatgtt cgttttccta ctttctccat tgttgttatt atttattcta
     9541 cttgttttta tcctcaaatg acaagtactt gaaaaaattt aatggtttct atacagctcg
     9601 ccattcttat tcgtgctcaa agaaaccatc tgctaaacta tattgtttcc ctgcgacaat
     9661 gtcatattgg atcaattgat attcgtcgta aatctcttgt tgcgagcgat tgaattgcgc
     9721 ttctttatac cactgtccgt tttgatagta cagttctcgt tcaaacgccg gaagttcctg
     9781 ttctaatgat aaaagcagct gataaaagcc atttgttgtc tgattcgtct gttccattag
     9841 attcgctgca aaataaaacg gactggtgat tgcatcgtga gtcgtttgct tttctaattc
     9901 tcctttactg tcgaacataa gaaattgtgt ttcatgcaat gtttgtttgt cattttttgc
     9961 ttggatcgta tctgaataga tccctggaag atgatccccc caaaaaacaa ctaaagtcct
    10021 tctagaaaga ttcttcaatt cttccgtgaa ctgcttcagt gccgtgctgc tataggaaat
    10081 atcttgcaga taattctcta agtcaagtgt cccacttcca tctgaagttt ttgcaggata
    10141 gtctagatta tcatactttc catcgtaggg catatgtgtc tgcatcgtga ctaaatgaat
    10201 gaactgaggt gttttttctt ccttgagaag tgtcattact tctttgtaag ctgactcatc
    10261 agaaatatat ggattattct ctatcgtatc cgtataggtc atcgtacgct cgctgatgaa
    10321 ctggtcaaat cctaatgtct gataaacatc ctctctttta tacatcgaag tattgtatgg
    10381 atggatcgct gtcgtctggt atgattgtgc gtttaacgct gaaacgatag aaggaagctg
    10441 atccattttt ggtacaagca tcgtgtaagg ggtggtgagt tgcgcattaa ataacgccat
    10501 agaaaaacca gtcaatgctt caaattcgat atttgctgtt ccaccgccat agttttgaga
    10561 aagcatattg ccactatatg tttgatcagc tacttcataa taatctgcca atggctcacc
    10621 agatacttct acgccgttta atcgactagg atctgagaaa ctttcactca tcacgaacac
    10681 gatgttcggc gactcttcct ctacagtttt atttttttca tcggctagct tctggtattt
    10741 ttcagtgatt tctttgatct tttctttcga atatccttca ggttcatcca ttggttctac
    10801 cttcagattg tacaaaaacc caccgataaa accagtgtta tagtagttca ttttctggct
    10861 gtaaggtatc catagtgccg ttttattata tgcttttcgc aacaaattat cgggattgtt
    10921 gaaattactg atatacccta gcagtccaat tgccacaagc agtgtcgtta atcggatcaa
    10981 ttggaatcgc cttccttttt tcaggcttcg aaacatgtac cagcaaaaca atcctatcac
    11041 ggttcctgcg gcagctaaga tcactgtaaa cagcatagtg ccggtcatat ctttcagcaa
    11101 tccgatttca gtgatcatct tcaaatcgtc tggataaatc ggttcttgcc gataactcat
    11161 tttcatataa tttgcaaaac ctaatacacc gatggctacg acatacgtaa gggaacctaa
    11221 agggacagat cctgctagtg caatcagaaa aataagaata atcagcaaga cgagacaggc
    11281 taagaagaat ttttccgtat gccaagaaaa agcaaatttc aacgcaaggt ctacggatag
    11341 atcattttga caccactgca aaaataaatt actaaaaaca acaagaaaaa agatactgat
    11401 gattgctgct gttcctttta aaatcttttt ctgtttggtc gaaagtctgc ttatatatct
    11461 tttcaaatgc ttgctccttc atttctatca ataaaagaat gttgcttctc ttattttagt
    11521 tgtacgaaat tcgaaagtaa agaacggaaa gaagaattaa tgaaatccaa atattctctt
    11581 tctttatggc ctttctggtt tctttaataa gcggaccttt cgtactccag aaattttttc
    11641 tagctgttgt ttcacttgat tgagctgttc aaaatcagta ctgtctatgt caatcaatat
    11701 gtagacatgc tcattcttat ggttacgcga tatatctgcg atcgctagct cgttctcatt
    11761 cagcagagaa aatatctctg ctaatatatt ggttttcttt tgataaaata tagtgaatcg
    11821 aaacggcgct tggaaaaaca ttcgtgcgga tggataattg accgcttctc taaccgtacc
    11881 gaacaaaaga taatctttta aggcttcaac tgccaaacgt ccgctgtctg ccaaagcttc
    11941 ctgagtcgat ccccctagat gaggcaacat aagtatacgg tcattttcta agaactcttc
    12001 ttctggaaaa tcagtcaagt atttagccag atgttgtttt tccaatgctt gcataacagc
    12061 atatttatca acaatttcac ttcttcctac attgataagt acagcatctt ttttcatttt
    12121 ttctatattc gcctgatcga tcaatccttt tgtatcttct gtcaacggca gcatgactac
    12181 gatgtaatcc gaggtagaaa gaactgtttc taaatctgct tgccgaacat aatccaactg
    12241 ggcatcgcgg atagaataac ccaagacatc catccctaag ctatagcaac ttagcgctac
    12301 ttttgtgcct atcgctccaa gaccaagaag accgatggtt ttgccttgca attctcgtcc
    12361 cacataagca cttcttttgt tttctgcctg ttctaaaata ttaggacctg ttagtgtttg
    12421 taccatacga cttgcttcga ttatcggacg ggaagataag agcaaacagc acaaaacaag
    12481 ctcttttact gcattagcat ttacacctgg tgtattcatg acgatcgtcc cgttttcaga
    12541 agctttttca acgttgatcg tgttcacccc aacaccagct cttgaaattg ctagcaaatc
    12601 tggtgtaagc cattcatctt tcactttcgt actacgaata actattcctt cggctggcaa
    12661 atcatctatt attttgaaat attccccttg taacttttct atctcttgtt ctggaatagc
    12721 atcaatcaaa tgaacgtgat acataaaacc ccctccttac taacaagtat ctcgaaattt
    12781 ctgcaattag acaactgttt accatttgac ttcctagaag caaaaagagc agttagagca
    12841 gagtaggctg agtgataaaa ataaggctat gacaagttac tgccataagc cttattttcg
    12901 agagcaaaat attgagggtg agatatcttg ataaatggtg ttccgttagc tgacattgat
    12961 aataagacta aatttcaaaa aagttgaaaa gcaaatttcg tcaatctctt cttatttctt
    13021 aaaactaagt ggctttttca caacctcttt ttatctaata accactgttt ccaactcaac
    13081 taattttgcc cacgtttcaa tttgatctgt tgttagattt agcgagacaa cggtatgatg
    13141 accgccacct gcctgaatcc atgaatggac accttcatgg aaattcggtt tagttttcca
    13201 taacacacgt gcaacaggta aattaggtgc ttcagtcgtt ggttcaaagg cttctacttc
    13261 attgatcagc agtttataat gcgtaccgaa atctgccatc gatacgacta ccccttctcc
    13321 tgctttccca tcaaatacaa gacgcgccgg atcttctcta tctcccattg ataaaggaga
    13381 aacaacgatt cttggtttcg tcgcagcaag cgtcggatct acttccatca tatgggattc
    13441 caagattgct tcttggccac tagctagctc gtaagtataa tcttccatga atcctgtatc
    13501 tttccctcga gccatgattt tcagcaaccg atcaatcgct gcagttttcc agtcgccttc
    13561 cccagcaaaa ccgtatcctt ctgccatcaa acgttgtaca gctagtccag gtagttgttt
    13621 cattccatac aggtcttcaa aattagtcgt aaaagcgtta tagcctcctt cttctaagaa
    13681 acggcgaata ccgatttcct gctgagcttg tacttttaca tgttcctccc atgtcttttc
    13741 ttcataatca ccatagtcga aggtatacaa ttccttgtac tcttcaaata attgatcgac
    13801 ttcttcgtct gatacgcgat cgattacttg tacaagatct ccgataccaa aataatcaac
    13861 agtccaacca aattgaatct gtgcttccac tttgtctcct tctgtgacac ccacgtttct
    13921 catgttgtca ccaaaacgag ccacttttat tccaaaatta tatgggattt tagattatct
    13981 ttttagaagt tcttgaatgt cggtatagcc cgtgtttccg ggcttttccg gcatttttga
    14041 tattgttaga tacttacaaa tgttcttatt acctctcacc atttccccct caaaagtagt
    14101 aaatggagta gtaaaaccgg ccttgtgtca ggctgccagc cgttccattt ctgcccttgc
    14161 ggaatcgaat gtggcgtgtg cataatagtt cagcgtcatg gtgatattgg aatgtcccat
    14221 aatgtactgc aaggctttgg ggttcattcc cgcattggct aaccgggtgc agaatgtatg
    14281 acgcatagca tggggcgtcg tcgttttcgg caacgcttct tcatggctct tgttgtactt
    14341 cttcacaagc ctgccaaaca tcccgccata atctaccgcc gtcatgggat agccgttctg
    14401 tttgagaaac aggaaattgg catacccgtc aataatgaac ggctttgcac ctttgcggtt
    14461 ttttatcact cgctggaacg cctgatacac ttcctcattc atggggattt ttctgacgcc
    14521 gctttgggtc ttgggttcgt caatatagta accgacgccg gtatttctca aaagctggtg
    14581 gctcacgatg attacccggt tttcaaaatc caaatctttg tccgtcagtc cgcacagttc
    14641 cgaaatgcga agccctgtcc ccagcagaat aacgattgcg tcataatgct tttggtaaac
    14701 cttatcacct tgaataaagg acagaaaact ttcttcctgt gctggtgtaa gaggcacctt
    14761 tggttccgta tcgtcgtcca gaacgtcact aagctggaag tcaaacgggt tttttcggat
    14821 gcaatcgtcc tgtatggcgg tgtaaaaagc cgctttcagg gaacgcttgt cgttattgat
    14881 ggtcttataa gaaaggccct tttctttcat ccgaagcgcc cattcctttg cgtcggacag
    14941 cttcacactg tcaatcgggc agctccccag ccgatcttgt tccagaatac gcatcagccg
    15001 ttcccggccc agtttggtgt tgtgcttcac attgccccgg tgcctgatct gcttggcgta
    15061 gagctggcag acggtcattt tcttgccgat ggtgtctatc ccgtcgtcaa ggtctttctg
    15121 gatttctttc tccttttccc ggagtgaaat atcctcccgt ttacctgccg gggtcttatc
    15181 cgtaggcact aacttccatg agtaaacaaa ctgcggtttt ccaaaggtat caacatattt
    15241 gtaagcatat cgtccgtctt ttctctggct ctctccagac cgcaaaacgc gatttttgct
    15301 gtctcgtctt ttttctgaca tctcgtcatg ctcctttcca tgacggaaag agccgcatat
    15361 atgcttataa attattataa catatctgcg gccctttttc actattagat tgtgtccaaa
    15421 gtgtcaatta ttttctcaaa ctgcttgcgt ttgatctgga tgcggttgcc attcataatg
    15481 acccagccag aggccggatt ttcctcggcc agcttacgca atttgttttc tccgatacgg
    15541 aaatatttag acgcttcctc aatcgtcagc gtgtattttt cccaaatagg cacatcatgg
    15601 ttcataatgc agcccccttt cacaaattca aaggtttgtg tgatttaggg gcatagacgg
    15661 acggctgctg gcacagctcc acgggacttt cacccccgcg tggttctcac gcggccctac
    15721 ccattgcctg cgacgctttt aacgctcgga ctgtggctat aagggagtat catagtcctg
    15781 cctgtacgtc gtcgcccgca agccgccacg ctcacattgc ggcgtggtgt tcctcgctcc
    15841 gcttttcccg tagggaaaag tatcatggcg caccagccgt ccggctcggc acagacagaa
    15901 tgtgtccgta tgccctatga tgcgccgccg ttatcttgcg ggcatattta tcaaggtgcg
    15961 atcaggccac cgggaaaaca ccgaaacggg gaaagggtaa gccgtttcat gctgccgggg
    16021 ccggttcgtc agttcgtgac gatagcgtgg atgagcttga tttccagacg gcgcttcatg
    16081 gcctcgtcca gacacacatg cggatagccg ttttcgtcat agagcgtccg ggtacacagc
    16141 ttgttcatgt aaccgctgta atactgcaaa acgcgctcta cggctccggc atccccagcg
    16201 cgggcggcct cgatcaccga aaggggcaaa agctcaatgc ccttgtagtt cgggttcttc
    16261 atccgcgtct aacctccctt cggcatcaac gcctttagct gtttccgcag ctccgataag
    16321 gtatttgtcc tgtgccgctg aacggcactc cgggacattc ccacaaggcc gccgatctca
    16381 ccatctgcca tatccagaac acagtgcagg attaaaatac tctgctccat gctgggcagc
    16441 gcggcaaagg cttcggcaac aagctcattg ttgatatgta ggtcatagcc atgcgacgaa
    16501 aacgtaaagc tgtcgctggg gtagtgatct actgtacaga gcttgtccaa atctgcctgt
    16561 gacaaagagc ttagtgacac ttcacggtca cggcggcgct tcatttcagc caagtagctt
    16621 ttggcctcgt tacgcagtac cgtcttgcaa aaagcatcga acatgcaccg cgcttcatag
    16681 tcgcggggga tagcatccat cttctcaccc cctttctgtg ggggacttag gtggttctct
    16741 ccctttccgc taacaagact ttgacaaggc cgccagctac ccgctaaata cctccttttt
    16801 gaaaatttct caaaaaattt ttttggggac agccgggccg caaaagacga caaaaatcgc
    16861 ccggcaggtc gcaaacctac cgggcgagaa aatgctttgc tatgctgctg atttacatgg
    16921 tatagtacat actcataacc ggaatagccc tgtg
//
LOCUS       DAYYGO010000063        16001 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000063 DAYYGO010000000
VERSION     DAYYGO010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.065"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            226..894
                     /locus_tag="ACHA5T_001724"
     CDS             226..894
                     /locus_tag="ACHA5T_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFM8887294.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1414)
                     /locus_tag="ACHA5T_001725"
     CDS             complement(1094..1414)
                     /locus_tag="ACHA5T_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887295.1"
                     /translation="MGDCFCDGRTSQRYQKNEEEARKQPLPKQQMTLQEIDSILTEAF
                     LSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYFMIDHQPILWEDVRYIELKETKK
                     WFVT"
     gene            complement(1451..2833)
                     /locus_tag="ACHA5T_001726"
     CDS             complement(1451..2833)
                     /locus_tag="ACHA5T_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFM8887296.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2989..4176)
                     /locus_tag="ACHA5T_001727"
     CDS             complement(2989..4176)
                     /locus_tag="ACHA5T_001727"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFM8887297.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4183..5214)
                     /locus_tag="ACHA5T_001728"
     CDS             complement(4183..5214)
                     /locus_tag="ACHA5T_001728"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM8887298.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5260..6684)
                     /locus_tag="ACHA5T_001729"
     CDS             complement(5260..6684)
                     /locus_tag="ACHA5T_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFM8887299.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMED"
     gene            6859..7764
                     /locus_tag="ACHA5T_001730"
     CDS             6859..7764
                     /locus_tag="ACHA5T_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFM8887300.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7855..8532
                     /locus_tag="ACHA5T_001731"
     CDS             7855..8532
                     /locus_tag="ACHA5T_001731"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFM8887301.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8529..8699
                     /locus_tag="ACHA5T_001732"
     CDS             8529..8699
                     /locus_tag="ACHA5T_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887302.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8813..9709
                     /locus_tag="ACHA5T_001733"
     CDS             8813..9709
                     /locus_tag="ACHA5T_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8887303.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9764..11134)
                     /locus_tag="ACHA5T_001734"
     CDS             complement(9764..11134)
                     /locus_tag="ACHA5T_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8887304.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11372..12118
                     /locus_tag="ACHA5T_001735"
     CDS             11372..12118
                     /locus_tag="ACHA5T_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM8887305.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12115..12954
                     /locus_tag="ACHA5T_001736"
     CDS             12115..12954
                     /locus_tag="ACHA5T_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM8887306.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12955..13905
                     /locus_tag="ACHA5T_001737"
     CDS             12955..13905
                     /locus_tag="ACHA5T_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFM8887307.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14046..14798
                     /locus_tag="ACHA5T_001738"
     CDS             14046..14798
                     /locus_tag="ACHA5T_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887308.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14817..15968
                     /gene="queG"
                     /locus_tag="ACHA5T_001739"
     CDS             14817..15968
                     /gene="queG"
                     /locus_tag="ACHA5T_001739"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFM8887309.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5193 a         2998 c         3223 g         4587 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttttcttg aaaatgccaa acatgttatc ctagagaatg ccggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt tgataccgtt
     1381 gactagttct tccatcgcaa aagcagtccc ccattttaat ccaaatggcc gatctcgata
     1441 ctcgttatag tcaatgaatt tttttgctgt ccgtctcatc ttttccacct tctaatccac
     1501 tcataccacc tgcatgcccg ccaacgagag aactacgtgc gatggcgcgt cctccttgca
     1561 agagactatg cgcatggatg attgattgaa agccgtattt ttttctaatc gtatcgacaa
     1621 caaaatcaac tttcgcttca tttatctgct tttcaggatc ttcaaaaagg ttaagctgta
     1681 aacctgtcgc gtatacgagg tcggaacagt tcacgcctac acgccgaata tcctggtagt
     1741 ggtaatgctg gtggaaaatg gtcaataaag catctgcaag atctctgcta gcatttgtcg
     1801 cttcgatttt catttgctgg tgaaacccgg tcttgccatt tggatcaaga tagcctaaag
     1861 agtatcctac ccataagctg actacttgtg tttttgctgc agatcgtctc aagcgagtgc
     1921 cgacttggtc ggccatttct ttgataacga tctcgatttc ttctcgtctt gtataatctc
     1981 tatctaggat ttgactattt ccgatacttt ttgattttgg tgtatattct tgtcccaaaa
     2041 aacttcgatc gatgccccaa gcatgggcat aaagctgagt accgatgacc cctagcttgc
     2101 ttttcaagtg ataataatcc gcgtgagcta aatcataaac agaatgtata cccatttgtt
     2161 tcagacgttt tgctgttcgt ttgccgattc cccaaaagtc agtgatatct gagattttcc
     2221 ataacttttc tggtacatct tcataacgcc attcagcaaa catttgcttt ccgtcttttg
     2281 agccattgtc taaggctagt ttagcaagta gcgggttatc gccaattcct atagtcgtgt
     2341 agattccggt ctgctcaaac acatctttat ggatcagttt agccatgtcg tacgcatttg
     2401 ttttaccaaa gagtgccatg ctatttgtta catcaaggaa gctttcatcg acggaataga
     2461 catgatggtt cgcttcgtct gcatattttt tatagataga attgatttct ctgttctttt
     2521 ccatataata ggccatcctt ggggcaacaa tatataaatc acttggatag gggaagggaa
     2581 gatctctggc acggctgata ttggtgatgc cgtaagcttt ttttgctgca ggactggaag
     2641 ccaagattaa tccgctaccc cgtgtttctg tcgaatcgga cggataggac atgactacta
     2701 atttagttgt aaggggatca agatcctttt caacgcactc tacggaggcg taaaaagatt
     2761 tgcagtcaat acataaaata tctcttgaag gttctttttc ataaacaaat accggatttt
     2821 gattcgccca cataacaatc acctcttcaa cacaattaga acacacgttc tctttccgtg
     2881 tcaataaaaa acagcaattt tcaaatacga gcaaaaggct cacatttgga aattgctgag
     2941 aggttatttt tatctatttg tttttagctg agatcgttga tttttttatc aaacatgaac
     3001 agcttctgga tcgatctttg ttgtcccatt tccaatgtgt gcaacataaa attccggagc
     3061 atagccgaca actttcatgt attcatcata gacattgttt ttgaaggcag gtatattttc
     3121 ttctttgaca agagcaatcg cacagccacc aaatccagct cctgtcattc gtgcacctaa
     3181 gacaccttct tgtttttgag cagcatctac tagtgtatct aattcgatcc cagttacttc
     3241 ataatcatag cgaagagagt ggtgagaatc atttaacagt tgaccaaatg cttctaagtt
     3301 cccagcttct aattcagctt ttgccttcaa agtccgctga ttttcagaaa cggcatggcg
     3361 agctctgcgt atcaatgtat catcgccgat ttggtcaata gttgattcaa attcttcttc
     3421 atctaagtct cctaaagaag agataggcaa agttttttgc aaacgtgcca aggcttcttc
     3481 gcattcgcta cggcgttcat tgtattttga atctgccaat tcccgccgct tattcgtatt
     3541 catgatgacg attgcataac catctagttt cactggaacc atttcgtatt tcagcgtatt
     3601 tgtatctaat aagattgcat gatctagctc accgaagccg atggcaaact gatccatgat
     3661 acctgaattg acaccgatga attcattttc tactttttta cctgtttgga ctagttccaa
     3721 gcgatctgtt tttaaatcaa acagatcttc taatacaaca cctactaata attctaatga
     3781 agcgctactg gaaagtcctg caccattagg gattgttcct tccactaata actcaaaccc
     3841 atgatcgatt gagtgtccag cttcttttaa tttaaggacc attcctttta cgtaatttgc
     3901 ccagtttgct tttttatcat attctagctc atctaaagta aaactaatga caccgatatc
     3961 gtcaaagttt gtggaataaa ctaatatttt attatcgtct cgaggtgctg ctacaccttg
     4021 cgttccataa gtgatggaag cggggaagac atggcctcca ttgtaatctg tgtgttcacc
     4081 aatcaaattg atgcgtcctg gtgagaagta ttgtgtagtt tttttaggtc cgaatgtttc
     4141 ggcaaatttt gtgtttaagc ttcttttgat ttccatgatg agttattcct ttcttgtttt
     4201 gattttgaat tctgttactg tttcaaatac tgatccggct tttaaatgaa tcgaaccaaa
     4261 tgctgagaag cgctctgcac ctggagctgt ttgagtctca aatgtgatgc caccgtgatg
     4321 tgccagtttt cggttgcgca tttctggtgt ttctgtgccg aaatttgcag tgaaaataac
     4381 cacactagaa gcatctgtag caatatctac ttggattctt ttatcggggc tagtcaagcg
     4441 agcggcttct ttacccaagc cggtttcttt taagaaaaat ggatgatcga ttccgtcaac
     4501 aagatttttt tgatccaaat cactagcaaa aacggacgct aattttttag gtatttgaaa
     4561 atcgaaagca cttcctgcag cattttcttt tacccctata ggaatactat ctgttcgcaa
     4621 aggagcataa gcttcgctat tgagccaaag ctcatgttga tcgattgact gagaagcatc
     4681 tcccgtcaag ttgaagtaaa catgatttgt cggattgaat aaagtatctt ggtcactggt
     4741 gccgcgagag gtcactcgcc agatattatc ttttgtcaaa gtatagcgga tttccacatc
     4801 cattgtacct ggaaaaccat gttctccgtc gggacttgta gtagtaaaaa tgacagaagc
     4861 ttcattttcg ccattcaaaa tgacataaga ccattttttt aattcaaatc caggagcacc
     4921 accatgcaga ttatgtccag tttttggatc tgtggctaat tggtaagttt ttccattgag
     4981 agaaaagcgg ccattttcga ttctccctgc ggtgcgaccg atagaagcac cgatatacgg
     5041 atctttttcg atatattctt cagcggtgtc aaatcccaaa actaattctc gatcatttga
     5101 ttttaaactg acgattctag cacctaaatc agagatagaa agtgctagct tgtttttgtt
     5161 ctccaaggtt atcaagtgat aaccttggcc aaaatctttt tctgtaatct tcattttcaa
     5221 gatcctttct taatgggaag gaactactgc atcgtgaagt taatcttcca ttgtatcagc
     5281 tggagccaat ttgttttcca atttctcaac gatttgtgca tgttttttct ctgtcaatgt
     5341 aatcttccaa gcaaaaataa tagcggagaa aatcatcaaa gcagcaggag catagaatgc
     5401 ataagctttg aaagtcgtta cgccatgagc agtgatatca gcagcagtag cattaccagt
     5461 catcccagaa gcgatagcga caaaccctac gatcccgtta gacaaagcac cagcgatttt
     5521 atctagtaaa gggcgaattg atagagtaac agcttcgtta cggataccat ttttccattg
     5581 accatattca acagagtctg tgatcgtcat caaagcggat aagaaaatca attgttgagg
     5641 gaagtagaac aatactagcc cgatgataac gattggcaaa cttgaacctg cgatcgtaaa
     5701 gaaaaagtaa gaagaaatca tcattgcgat ccctccaagg taaacgtagc gacgagagat
     5761 gacgcgtgta agcattggga atagtggaac agctactaaa ccaagtaagg cagcaactag
     5821 accaacgatc caaaattcag ttggttggcc aagtacatat ttgaagaagt agaataatac
     5881 ggcagttgtt gctgcattgg caatcgcata agcgatataa gcaagggcaa gccacatcaa
     5941 ctgatcattt tgagcaactg ctttgaacac atctttgatc ccagcttttt cattttgctg
     6001 acggatcgca gtatcgactt cttttgtgcc caaggcagtg ataatcgcag taccgcctga
     6061 aataacagca gcaatcacac cgaaccagaa ccagcctgaa gcgccttcaa tatgtttatg
     6121 agtaacaaga taggtaaaga aagtaacgat agggacaacg actagagtag taccatttgc
     6181 acctagagaa gagccgaaac gagcaaacgt agcaaatttt tcacgttcct ttgtttccgt
     6241 actcaaagct ggaatcattg accaaaaagc gatatcctta aatgaataaa aactatccaa
     6301 taagatgaaa acgattgcaa ataatatgat aaataaagtc gtattatgag tagctaagcc
     6361 aaagaaattg gtaaagattg ctaccaagag tacggaacta acaacgccgc cgataactaa
     6421 ccatggtttg aatttaccgt attttgtacg agtattatcg atgatgccac caatcaaagg
     6481 atcaaaaata atttcaacta agcggatacc tacaactaag cttgtaactg tagcaatcat
     6541 ttttgcattc gttgccttat ctgttcctgc gaacatctgg cttgtcacga ataccataaa
     6601 ataagtgctt aatgtaacat agaatgtgtc gtgtccaaaa gcgcctaaag cgtaggacac
     6661 acgttggatc attttgcttt tcataatctg tattcctctc ctgttcaata tataatacta
     6721 agtaaaccgg ttacaacgaa taacaaagat cttttaatga cttaaactta acaagaaatc
     6781 gcttcctttt taatatattt ctgtgacttt tatttaactt tttgttgcat tatactaaag
     6841 gagtaaggag ggatagacgt ggagcaatcg atttttttaa aaaaggaaca tcaacaagta
     6901 gaaaaagaat tgtattttga tttttgtggt ttctctaaaa cgatgccgtt tcattctttt
     6961 ggtcctgcta ttcgcaatga ttacatcctg catgtcgtga tggaaggcaa aggatcttat
     7021 catgtgaaaa atcaacaatt ccaattgaaa aaaggtgatc tgttcttgat tcgtccagga
     7081 gactcgacgt tttacttatc agatggagaa gatccgtgga tgtattgctg gctttcattt
     7141 ggtgggagtg ctgcagaaga aatcattcgg cagtcgcttt tcagagaaga ccaatacacg
     7201 atggtctctt ctgacataca aaaatatatc gacattattt tagaatgcat gaaagatacg
     7261 gaagaaacct tggaaaatga attacagtta acagcattaa cctaccgttt tttatcagtc
     7321 cttttaaaag atggcggaaa ggtcagcggt ggtgtacaaa aggttcattc ctctttagct
     7381 gttgaaacag tcaaatacat cgaggcacat tatgctgaaa aattgtctgt tgaggaaatc
     7441 gcccagcatt tagcggtcaa tcggagccat ttgtcgcgag tattcaaaaa tcatatgggt
     7501 accagtatca aggaatattt gatcggggta cggatcaatc gaggggcgtt tcttctttct
     7561 ctgacggatg aatccgtgga aagtattgcc tatcaagtag ggttcaatag tctagtcgta
     7621 tttagccgaa tgttcaaaaa gagtaccgga gaaacggcta cgaactatcg taaacgtatg
     7681 aaaaacgaaa attttaagaa tattgctttg gataccttga aaaaacagtt ggaaaaacaa
     7741 accgcagttt cttggacaac ctgattagtc tggttaaaat gacaagcatc ggcaaaagaa
     7801 acttgtggcg gttatgctac actgtaaagc gaagactaaa aaggaggaaa agggatgcgt
     7861 acacttatca tcattagcca tccaacgatc gaagaatctt taaatcagca atttttcaaa
     7921 gaagcttctg ctggtttacc agctacttgg catcatttgg aatcaagtta tccagacgga
     7981 aaaatagata tagagactga actacagctc ttggcccagc atgatcggat cattttccag
     8041 tttccgtttt actggtatag ttctcctgct catttaaaaa tttggcaaga taatgtcttg
     8101 gaacgagcag cacatgtatt aaaagggaaa gaactaggaa tcgtattgac aacaggtgtg
     8161 gcggaaaaag aataccaagc tggaggaaaa gaagaatata cgatttccga atttttgagg
     8221 ccgtatcagc gaatcgcaaa taaattccat atgacctatc ttccgccatt cgttttagca
     8281 caatttatgt atttatccca ggaaaaacgt tgggagaagc taattgctta tcagcagtat
     8341 ctctcattgg aaggcaaacc atcgctgact cagcggattg actggtttat tcaaagagta
     8401 caggaaaatc agaaaatgca ggaagaagaa tctgaaaaac aagcatatat cgtagaagca
     8461 ctgactgagg caaaagagca gatcgaagac ttgaccttta cactgcaaga aatgaaagga
     8521 acttcattat gacaaatgat tggctgatcc aacaattaga tgaattaagc gaaaaatcag
     8581 aatatcaaga ccgtgcgctc ttatatgaaa taaagaaact gatggctgag atagacgaac
     8641 gaacaatgca gctgcaagga gaaatcgacg gacaatcttg gaatcatctc aactggtgaa
     8701 attttcttat aaataacaaa gataaaaatg tgcacagcga caattatttg ctacaataag
     8761 aatatgaagc gtttgataac gctattatac ataaaaattg gtggtgaaaa aaatgtatga
     8821 tgaaaatgag tggggagaaa atgttcggat gacccgaaga agacagcaaa aacaggcagc
     8881 tcgtaagcgg atgatcatga ttggggcagc agttgccgtg tttttgatca tagcaggagg
     8941 agttgcgggt ctatattttc atggaaaaaa ttcgtcagaa gcgaaaagta cgaccataac
     9001 ggaaacaacg acaaagaaaa cagagacgaa acaaacaagt gagtcatcaa ctaaaacaaa
     9061 agagacacaa acttctgaag aaaaaacagc aaatggtttg ccagagcaac ttcaagcaat
     9121 cgtaaatgat tattcgaaaa agatcgaaga aaagacgcct gcattgattg aagaatacca
     9181 gacagaaatt cagggaaatc aagagggaat agctggttta tcagcagttg ctaaccaaaa
     9241 agcacgtgag cttcaagcaa tctccgacga agggatcaga aaattacgtg cagcgtatca
     9301 ggcagcagaa aataaagacg gtgtagactt agatactttg atcaatcaat tatcggctaa
     9361 ttatacgaat catgtcgcta agatttccga tatttatttg cagactagcg cctctcttca
     9421 agcagagtca acaagcacac aagacacaac atcttctgat tctgagacag cagagtcggt
     9481 agaagcttct gaaatgcaag ctcgaacaac acaagacaca acagattcct ctgaaactaa
     9541 taccaacagc gcgacttcac aggctgcgac aaccgtagtt ttggaaggag aaggtccaaa
     9601 tcaaatagcc gaacgaactg gcgtaccagt agaaacgatc ttgtcgctga atgggatgag
     9661 catggagaat tatttcctga ctcccggtga gacgctaaaa ttgaattgat aaaagcaata
     9721 aaaaagaacg aagcttccac taaggtgaag cttcgttctt tttttatttt ccggttcttt
     9781 tttcgcgtac aaagtaatgg atcgtcatgt aaagcaagaa aacgagtcca ataatcaatg
     9841 agattctcgt ttcaggattg ataaacatga aaatcaatac gatagctaaa aaagcaagtg
     9901 tcacataatt cgtataagga gatagtggca ttttaaaagg atgatcatgc attttatctg
     9961 cgtgcttctt tttgaagcga atctcgctga tcaaaatcac tacccaagga accataccag
    10021 gcaaaatact tgagctgtat acgaaaacga aaatattttc tgactggttg atgaacattg
    10081 gtagaagata atttaagatc agaccaatta agattccacc tgagattgct acgacaggat
    10141 aaaaaggcgc accgtgacga gatagtttca gaaatttttt agacatcttt ccatttatgg
    10201 caagtgtata gaccatacga cttgcgctga aaatccctga attgcaacct gataatgccg
    10261 ctgtaatcac gacaaaatta atcagtcctg cagcaaatgt aatgccgact ttagaaaaag
    10321 tttgtacaaa aggtgaacca atctcactca attgattcca cggatagata ctcacaatga
    10381 taaaaatagc accaatataa aaaatcaaga ttcgaccgat agtggactgg acagcttcaa
    10441 tgatcgtctt tttaggattc tctgcttcac cagcagtcat tccgatcagt tcgattcctt
    10501 gataggaagc aacaacgatc gataatgcaa agaaaaatcc tttgacgcct ccagtgaaga
    10561 atccaccatt tttccaaaga tttgaaatcc ctactgcctg accatgatta ccaaatccaa
    10621 ataagattac cccaagacct gcaatgatca tcaaaatgat cgttatgact ttgatgatcg
    10681 aaaaccaaaa ttctaattcg ccaaacgcct tgacagaaat aagatttgcc agcataagaa
    10741 acaagacgac aatgattccg ggaataattg ccggcaaatc aggccaccaa taattcatat
    10801 aagatcctac agcaatcact tcacttattc ctacgacaat atattgaaaa atattactcc
    10861 aagcggtcaa gtagccagcc acaggatgga tgtactcgct cgcgtaattt gcaaaagaac
    10921 cagtcgaagg atcaacgtaa agcatttcgc ctaaagcacg cataatcaaa tacaaaaaga
    10981 atcctgcaat cgcataggct cccatcactg agggccctgt ccaacgtatg gttgaggagg
    11041 cacccataaa cagaccgaca ccgatcgttc cacctagtgc gatcatttgg atatgacggg
    11101 cactaagact tcgagttaac ttttcttctt ccatacaaaa actcctttcg ttgttgagtc
    11161 aacaagaaaa aataatcaaa cgtttcaaca catgtttttc attattaggt tagccttatt
    11221 gaactcttga tgtgattcta cagaaaattt tcataaaatg cgaggaaaat tcgaaaaaaa
    11281 ctttttcatc attctacttg aaaatgcttt ccattttagt taaactatat ttaaataaaa
    11341 agttaggtaa acctaaaaag gagtgtaaga tttggaacgt acggccattg taattcaaga
    11401 cctttcagcc gcttatcaag ggaaaactgt tttgaaaact atcaacttga cgataaatcc
    11461 acaaaaaatt accggtatta tcggaccaaa cggtgcagga aaatcaacat ttatgaaatc
    11521 tttgctagag ttgataccag ctactactgg taaagtcacc ttttctggac agtcggtaaa
    11581 ctcagtccgt aaaaaaatag cctacgtgga acagcgaagt gaattggatc tttcctttcc
    11641 aattgatgta ttaggcgttg tacttttagg aacatatcca tctttacgaa ttggacaaag
    11701 acctgggaaa aaagaaaaag aacgtgcaag acaagctttg aaaaaagtag ggatggaaga
    11761 atatgccaaa agacagatca gcgaactatc gggtggacag cttcagagag tttttattgc
    11821 aagagctcta gcccaaggag cagaatggat ctttttagat gaaccattcg tagggattga
    11881 tgcgttaagt gaacgaaaga tctttgacat cttgcaggaa ttgaagaatt caggaaaaac
    11941 gattttgatc gtccatcatg atcttcataa agtagaagaa tatttcgatg aggttattct
    12001 tttaaataaa cagctgatcg cttccggtcc agtacaagaa gtctttacat cgaagaacct
    12061 tcaattggcc tatggtgaga tcatcagaca tttagtaaaa ggaggagaga aaaaatgatc
    12121 caatccttta ttgatggatt aatgaattat caattcctgc aatacgcttt ggtcacttcc
    12181 atgcttgtag gacttgcttc tggagtcatc ggttctttta ttattttacg agggatgtct
    12241 ttgatgggag acgctatctc tcacgcagtt cttccaggtg tagccatttc ttatatgttt
    12301 ggttttagtt acatctttgg agccactgct tttgggatgc taacagcagc agtgattggt
    12361 ttcgtgactc agcggagccg attgaaaaat gatacagcga ttggaattgt ctttagctcc
    12421 ttttttgcat tagggattat cttgatttca tatgcccaga gtgcgacaga tttgtatcat
    12481 attttatttg ggaatgtctt agctgttagg gaaagcgatt tgttgctgac agcactcgtt
    12541 agtggaatcg tcttgatatt tgtctttttc ttttataaag aattgaagat cacatcattc
    12601 gatccgacaa tggcaaaagc ttatgggctg aacacatcgc tgatccatta tcttttgatg
    12661 ttctttttga cattagtggc tgtagtcagt ttacagacag taggaacaat cttggtgatt
    12721 gccatgttga tcacaccagc cgccacggct tacttgctaa cgaaccattt actgaaaatg
    12781 atcattacag ctgcaggaat cggtatgcta agtgcagttg tcggtgtgtt tttcagttat
    12841 agttacaatt ggccatcagg agctacgatc gtgttagcat gtaccgcatt ttttatcctt
    12901 gcttttttat tttctccaac aaaaggaatt ttatttcaga aaggaagcag atagatgaaa
    12961 acaaaaaaat cactcttttt aatcttggca gttagtttct tggtattagc cggttgcgga
    13021 aagcaggctt ctgaccaagc ggatgaagga agtaaagaaa agttgtcagt cgtggctacc
    13081 aattcgatct tggcggacat ggcaaaagag gtcggaacag atcaaataga tatccacagt
    13141 atcgtaccag tcggaacaga tccgcatgaa tatgaagtac taccagaaga catcaaaaag
    13201 gcaagcgatg cagatgttat tttatacaac ggtttgaatc ttgaaacagg taacagctgg
    13261 ttcgataact tgatggaaac ggctaaaaaa gaagaaggaa aagattattt tgcagttagc
    13321 aaaaatgtag aacctcttta tttaactagc ggtgaagaac atacaaaagc agatccccat
    13381 gcttggctag acctatctaa cggaataaaa tatgtggagg aaatcgcacg tatattctct
    13441 gaaaaagatg cagaaaatgc gacactctat aaaaaaaatg cagaagcata tgtggaaaaa
    13501 ctaaaagaat tagatacaca agcgaaggaa agttttgctt ctatcgaaga gaacaaaaaa
    13561 ttattagtaa caagtgaagg ggcattcaag tatttttcac gagcatatga tttgccagca
    13621 gcttatatat gggagatcaa tacagaaagt caaggtacgc ctgatcaaat gaaagcgatc
    13681 attgatcaga taagagcaaa agaagtaccg gttttattcg tggaaaccag tgtagactca
    13741 agaagcatgg aacgggtagc aaaagaaacg ggtttgaaaa tctatgataa actgttcact
    13801 gattccatag caaaagaagg agaacaagga gattcttact atcagatgat gaaatggaat
    13861 attgaaacga tccatgaagg tttgtcacaa acaaaagaaa gctgaaaagt gcaaggtcta
    13921 gcttgaggag cagaccttgc attttttata agagatatga aaaaaaatta tgctgttttt
    13981 cgtcgtacag ataccttttt tgaagaaatt ccgttatagt tagagtaagg aaggaaggtg
    14041 tggctatgaa ttttacacgt aaagataaat atacgattaa agaagaagtc tttcgattga
    14101 tcagccagca ggaaaatacc gacaaggatc aagaccttca atttcttgaa gcagaattag
    14161 ctaaattatt catagcaaat caaaatgaga caacagaacc agatgaagag ctgtacatag
    14221 gcaaatggtt gtttgatgaa tttcagcctt tagatgaaaa taccggtatc ggttattttg
    14281 tgttcctttc tccagtcggc ttcacacctg gcgtattagt taaacaaaac gacgaaataa
    14341 ccgtcaaagc atggcctgca ttattttcag tcatcaaagc agaaggagta atcaaaaaga
    14401 tccagtcggc ctattataaa agtcaattta ttgccaacag caaattaatg aatatgtttt
    14461 ataatcgtct agtagaaatt gctaggcctg ggaaaatccc gcaaacattc acgtacacac
    14521 agcaaaaaaa tctctatgaa gaagtattgt attcccgcga tctgtctgag ttttcgccat
    14581 gggaagaggt aaggctccat gaactgttaa gtacaaaacc agtttcattg aattttttta
    14641 atgcattaga taacaaaaga aaattaattt caacaaatta ttgggaagta tttgctgcaa
    14701 gaaaaaaatg gatggaagaa aagaagattg ccgatcagca gatccagttt gtccagctga
    14761 aaaaagaaaa actatattat gggttataca gccgttaaca gcaagcgagg aagaccatga
    14821 cattaaaaga agaaatcata caagaaagca aacgattagg aattgacaaa atcgggttca
    14881 ccacggcgga accttttgat tcattaaaag aatcgttgga agaacaaaaa gcagctggcc
    14941 atacttctgg ctttgaacat ccaaatatcg atgagcgtct ttatccagaa atgacatttg
    15001 atcagccgaa atcgatcatt tccattgcac ttgcgtatcc tactaggatc catgaagaag
    15061 tgcctaggga tgaaaaacgc gggcagtttg ctcgtgcttc ttgggggatc gattaccatg
    15121 atatactcag agaccgtttg aaccgattga tcgaattcat ccaaacaaga gcagaaaaat
    15181 ggcaaaaaga agaggaatgg cgtctagctc cgcaagtaga tactggagaa ctgatcgatg
    15241 tcgctgtagc acagcgagct ggtttaggat ttattggacg aaacggattg ttgatcactg
    15301 aagagttcgg ttcatttgtc tatcttggtg agatcgttac taatattgta tttgagcctg
    15361 ataaaccagg agaatttggt tgcggtgact gtatgcgctg cgtgactgct tgtccgacaa
    15421 aagcattgct tggtgacgga agaatgaatg cccagcgatg tttgtcttat caaactcaga
    15481 caaaagggat gatgccggaa gaatatcgta aaaaaatgcg aaatgtcatt tatggatgtg
    15541 atatctgcca gttagtctgc ccttacaatc aaggaaaaga ttttcatttt catgaagaga
    15601 tggaaccaaa agtagacgaa atatatccaa aattaaagcc aatgctttcg atgtctaata
    15661 aagacttcaa acagcaattt gggcatttag ctggttcatg gcgtgggaag aaacctttgc
    15721 agagaaacgc attgattgct ttagctaatc taggtgatcg aacggcattg cctgagattt
    15781 cagaatgttt aacagatgtc cgccctgtca tcagaggaac agcagcttgg gctattggac
    15841 gtctaggaac aaaagaaccg gaaaaatggc ttgatgtatt agatgaaata ttagctaaag
    15901 aaacagaaga agtcgttata aacgaattgg aacatgccca gaaattattg cggagaaagt
    15961 taaaataaaa aaatagttaa cattattcaa agtttttgtc c
//
LOCUS       DAYYGO010000064        15929 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000064 DAYYGO010000000
VERSION     DAYYGO010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.066"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..116)
                     /locus_tag="ACHA5T_001740"
     CDS             complement(<1..116)
                     /locus_tag="ACHA5T_001740"
                     /inference="COORDINATES: protein motif:HMM:NF045046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate--amine ligase"
                     /protein_id="HFM8887310.1"
                     /translation="MNILILSCGRRCELVNAFKTALNNNEMEGKIIGADCSEL"
     gene            complement(128..1945)
                     /locus_tag="ACHA5T_001741"
     CDS             complement(128..1945)
                     /locus_tag="ACHA5T_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFM8887311.1"
                     /translation="MFILTRRRKIATLVFVDSLLLIAANIAAAKFMKPFVSIPMDLIL
                     ISIGLSIGFYLFYGSLFKVFTRINRYTNLREIIAIFSSLSASAASSILILLFINRRYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYIETKNMRYVSADSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIHVVGFVDDDPNKRNTYLSGKKVLGALKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESARVKVNTMPSIEELASGKISVSKLKTIDVVDLLGRDEVKLDIESI
                     KDQITDKVILVTGAGGSIGSEICRQIIQFNPAKLLLLGHGENSIYLIDRELRTHHQNC
                     PTEIVPIIADIQDREKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAAKVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAQGGPITVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSIQKVMDFVKSLPQDDEQLAKDIVTFANASNR"
     gene            complement(1959..2723)
                     /locus_tag="ACHA5T_001742"
     CDS             complement(1959..2723)
                     /locus_tag="ACHA5T_001742"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFM8887312.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMTASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPDYEEVKKKKFGLF"
     gene            complement(2751..3449)
                     /locus_tag="ACHA5T_001743"
     CDS             complement(2751..3449)
                     /locus_tag="ACHA5T_001743"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFM8887313.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAR
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILDEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLNKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(3461..4240)
                     /locus_tag="ACHA5T_001744"
     CDS             complement(3461..4240)
                     /locus_tag="ACHA5T_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFM8887314.1"
                     /translation="MEETISLKEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDALGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(4256..5200)
                     /locus_tag="ACHA5T_001745"
     CDS             complement(4256..5200)
                     /locus_tag="ACHA5T_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM8887315.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTLQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(5246..6025)
                     /locus_tag="ACHA5T_001746"
     CDS             complement(5246..6025)
                     /locus_tag="ACHA5T_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887316.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKNFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVVIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISAAYATKAQTVAQQMGYSNISY"
     gene            complement(6415..8490)
                     /gene="glyS"
                     /locus_tag="ACHA5T_001747"
     CDS             complement(6415..8490)
                     /gene="glyS"
                     /locus_tag="ACHA5T_001747"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFM8887317.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRASRGHRFLGEDVTFTHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(8492..9409)
                     /gene="glyQ"
                     /locus_tag="ACHA5T_001748"
     CDS             complement(8492..9409)
                     /gene="glyQ"
                     /locus_tag="ACHA5T_001748"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFM8887318.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            complement(9793..10605)
                     /gene="recO"
                     /locus_tag="ACHA5T_001749"
     CDS             complement(9793..10605)
                     /gene="recO"
                     /locus_tag="ACHA5T_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFM8887319.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(10748..11647)
                     /gene="era"
                     /locus_tag="ACHA5T_001750"
     CDS             complement(10748..11647)
                     /gene="era"
                     /locus_tag="ACHA5T_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFM8887320.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(11662..12057)
                     /locus_tag="ACHA5T_001751"
     CDS             complement(11662..12057)
                     /locus_tag="ACHA5T_001751"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8887321.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(12041..12517)
                     /gene="ybeY"
                     /locus_tag="ACHA5T_001752"
     CDS             complement(12041..12517)
                     /gene="ybeY"
                     /locus_tag="ACHA5T_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFM8887322.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(12534..14726)
                     /locus_tag="ACHA5T_001753"
     CDS             complement(12534..14726)
                     /locus_tag="ACHA5T_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFM8887323.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(14750..15721)
                     /locus_tag="ACHA5T_001754"
     CDS             complement(14750..15721)
                     /locus_tag="ACHA5T_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFM8887324.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
BASE COUNT         4243 a         3387 c         2865 g         5434 t
ORIGIN      
        1 agttcagagc aatctgctcc aatgattttt ccttccattt cattattgtt taatgctgtt
       61 ttaaatgcat tcactagttc acaacgacgt ccacaactta atattaaaat attcaatttt
      121 taatctccta tctattcgaa gcatttgcaa aagtgacaat gtctttagcc aattgttcat
      181 cgtcttgcgg caggctcttg acaaaatcca tcactttttg tattgagtaa cctttgatgt
      241 ttcctacaaa gattttctca taaactgctt catcatttct ttccttatcc aacaatagtt
      301 cttcatatag tttttcccct ggacggatac ctgtttcaat gatttcgatt tcgtcttcgc
      361 tatacccgct caatcggatc atgtttttcg ctaggtctac aatcttcact ggttcactca
      421 tatctagaat aaaaatttcg ccaccttttg ctaatgcgcc agattgaata actaaacgac
      481 tagcttcagg aatcgtcata aagtatcgag tcatacgaaa gtctgttaca gtgattggac
      541 caccttgagc aatttgttct ctgaatactg gaatcacact accgcgagaa ccaaggacat
      601 tcccaaatct gacagctgag aatttcgtac agccttcttc attcaatcca gtcacaatca
      661 tttcagcgat tcgttttgtt gaacccatga catttggagg attgttagct ttatctgtcg
      721 ataccatgac gaagtttttc acttttgctg ctttagctgc ttctgctaca ttttttgtac
      781 cgaaaatatt gtttttgact gcttcttttg gattatattc catcagtggt acatgtttat
      841 gagcagctgc atggtagacg atatctggat gatattgttc catgatctca ttgattttct
      901 cgcgatcttg gatatctgcg atgatcggta cgatttctgt aggacaattt tgatgatgcg
      961 tgcgtaactc acggtcaatc aagtagatcg agttctcccc atgtcctaaa agcaatagct
     1021 ttgcaggatt gaattgaatg atttgccgac agatttctga accgatcgat cctcctgcgc
     1081 cggtgacaag aatgactttg tctgtgatct gatctttgat ggattcaata tccagcttta
     1141 cctcatctct tcccagtaaa tccactacgt caattgtttt taattttgat acgctaattt
     1201 tccctgaagc aagttcttct atagaaggca tcgtgttgac tttcacacga gcggattcga
     1261 ctaactcgaa gattcgtcgt aatttttttc ttgataagga agggatagcg atcgttacca
     1321 tttgaatgtc atatttttca atcaattccg gaatatcttt taatgcacct aatacttttt
     1381 ttcctgaaag atacgtattt cttttatttg gatcatcatc tacaaacccg acaacgtgga
     1441 tatcttgagc cgttttggat ccaagaaagc tgttgtataa aatacgcccg ccttcgccag
     1501 caccaacaat caatgtattt ttagcagaat ctgctgatac gtatctcata ttttttgttt
     1561 cgatgtagat acgccagatc aatcggctac cgatgatcaa taagagcgaa agcagataag
     1621 caaagataac taaccgtaaa ctgtatcgcc gattaatgaa cagcagtatg aggatacttg
     1681 aggcagcact tgcagatagt gaactaaaaa ttgcaatgat ctctcttaaa tttgtatagc
     1741 gattgatccg agtgaaaact ttgaataaac taccataaaa taaataaaaa ccaatcgaca
     1801 aaccgataga gattaaaata agatccattg gtatagatac aaacggcttc atgaatttag
     1861 ctgctgcaat atttgcagca attaataaca agctgtccac aaaaaccaat gtggcaattt
     1921 ttcttcttct tgttaatata aacacttttt aattccccct aaaataaacc aaacttcttc
     1981 ttttttacct cttcatagtc tggataactg atttcatccc cgttgatcag atcctttgca
     2041 acttgcttca tctgtactac tttttcctta ccaaagtcct tggcaatctg ctcatagcac
     2101 tcttttaagt aaaaggtgcg cttagagact ccgtgtgcat cagaagcagt catctgcacg
     2161 agattatgtt tgaccattgt tttagctgtt ttctgaatat ctttgccaaa taccccaaca
     2221 taacttggcg cagtaagttg ggccaaacac cccatatcta ggaacggaag caaatgattc
     2281 gggtcttcgc gaaaatgcgc gttgcgttct ggatggacga tcactggtgt ctttcctagt
     2341 tctaacaact caaaaaaaag ccgttctgca tataacggca cttccagtgt cggaaattcg
     2401 atcagaagat atgtatcatc taaatctgta aataaaattt catctcggtg gatgtcttcg
     2461 atgagcgttc cggtaatacg tacttcttgt ccttccaata acgtcaaagg caactgtctt
     2521 ttctctaatt cggcttgtaa cgaagcgact agagaaatga cttgtgattt ttcattgctg
     2581 tattttccat tattgtggtg tggtgtgcat aaaatatgcg tgatgccttg ctgaatcgct
     2641 ttttcagcca ttgcaatact tgcttctaag ttttctgccc cgtcatcgat tcctggtagg
     2701 atatggcaat gtagatcgat catttctctt gtctcctctc ttcttattcc ttaatttccg
     2761 tagtaataac cagcatcttt gctatgttct gctccgttgt atacaacacc taaaactcgt
     2821 gcttgtacca tctctagcag ttcttttgct ttattcaatg attctttacg tgatgtattt
     2881 tcacgtacga ccaataaagt gccatccgtt tttgaagaca tgatctgtgc atctgttacg
     2941 gctactactg gtggcatatc gaatatcacc acgtcaaata actgtcttgc ttcgtctagg
     3001 atctgatcca ttcttggcga acttaacaat tctgatgggt tgggtggctt aggtccacta
     3061 ggcaaaatcg acaagttctc gactggcgtt cgttggatta catctgctac acttcctgat
     3121 gaactaagtg ctgtacttaa tcccctggca ttgttcagct ggaatgtctt atagacgacg
     3181 ggtttacgta aatctgcatc gactagtaga actcgttgac cggatttagc aaaaacgact
     3241 gcaatgtttg ctgcagtggt ggattttcct tctccaggac ctgatgaagt cacgacaatc
     3301 gttttgattt gctgaccagc tgcggaggca aactggatat tcgtccggat cgtacgatat
     3361 tgttcggcga ttggtgaaga tgggtttgtt aatgtgatca aactgactgc atgagtttgt
     3421 gctgcttttt gtttctgtgt tcgtgccatt tttcttcctc ctagactctt gagcggcttc
     3481 tgcgtgatgg ggctgtttta tcgtttgtag cttttggtcg tgcaggagct gttccttttt
     3541 tacggatacc agcgttcaat tctttcgctg acatttctgg aactgttcct aagattggga
     3601 atcctaatgc atccgtcaca tatttttcat ctttcactgt ccgatcaagt aattcgaata
     3661 agaatgccag acctacgcct accatcattc ctaatactaa gccaatcact agattcaatt
     3721 tattatttgg cgaaacaggt gtcgtattgg caactgcacc tgaaataatc gagattttat
     3781 cgacattcat cacgtctttt gcgttctctt ggaaaacttg tgaagttgtg ttagcgatct
     3841 gttgagcagt cactgcattc gtgcttattg cctggatcga gaacatttgt gaattttgtg
     3901 actgattgac ggagatcgca tctttgattt gtccagcagt cattttcaca ttgtcttctg
     3961 tttctaaacg atctttgact tcatttagta ctaaatcact gacgatcata tctttatacg
     4021 tattgatcat ttgcaggttc gtattgacat cattgacatt tgctgtgtct gattgtggca
     4081 gtgtcacgat caattgtgct tgtgaactgt attttggtgt caagatgaaa aatgttacga
     4141 ttcctgagat ggccaagcca gcaaacattg agatgatgat tgttaccaag tgtttgcgta
     4201 atgtttcaaa aatttctttc aagctgattg tctcttccat tttttctcca ctttcctatt
     4261 tattctctag attcatttga tcttttaatt cttttttcac acgatcaagt tcttgatcgc
     4321 tgactttttg ataggagata ttatcttgca tgaagccttc tccttgcagc tgatcttgct
     4381 tgatcgtacc aaatgcctcc cgataatcta aagcgatttt tttcatttca tcaaagctca
     4441 tgtctgtttt catattcgac tctagagcat tcaaaacgga ttgatagttg ctgacaccgt
     4501 ttaaactcaa tacttttttt gctacacctt ctacgatctt tctttgacgc tcttgtctac
     4561 catagtcacc atttggatct tcataacgca tacgagaata agataatgcc tgatccccgt
     4621 tcaatgtaat tttaccgatt ggaaagtcat agccatcttg tgagaaagtg aggtcattat
     4681 tgacttcgat accaccaact gcatcaacga gttcctttag ccctttcata ttgatagaga
     4741 catagtgatc gattgggatg tctacatatt tttgtagggt atccatcgcc atggcagctc
     4801 cgccgaatgc gtaagcgtgg ttgatcttgt cttttgtgtc acgacctacg atgtcgacat
     4861 acgtatctcg aggaatgctg acgatcgttg tctgtttgtc ttcaggactg actgtggcaa
     4921 ccatcatcgt atctgatctt ccttgatcga cccgacctaa atctccagta tcaatgccca
     4981 tcaatagaac agagaaagat tcttgtttgt ccagatcaac tggtttcgtt cgttctcctt
     5041 gggttcgttc cactgattcg taagttttct caatagaatg tgagagatcc agatataatt
     5101 ttgctcctac tcctacaatt gctaatacta atacgataag aactgctaaa ataatcagaa
     5161 atattttttt ccctcttgat tttcttcttc gtcttgccat agtttcctcc gtttgctaac
     5221 cttgcttgaa tcaacgtttt aactgtcaat aacttatatt gctatagccc atttgctgag
     5281 caacagtttg tgcttttgtt gcgtatgctg cgcttatttt ttcattccgg atctgctcta
     5341 tttgactgag cgccgtcaaa tattgatcgt aagtagcggt ggcagtcact tgaccatcct
     5401 tcaacatcgt atcgaataac tgatctagtt gcgtgacttg cgcgatctga gtatcagcga
     5461 atcctagata ttgcataaca accgttttcc aaggactatc tttaaaaaag ctcatatttt
     5521 cacgtacacg agcgacatct gcacttgtca ccttttcttt gatgacgaca tcgtctacag
     5581 cttgctgcca gtttgataca tttttagtaa ataaatcgtt gacagctgtc tggatcttca
     5641 gttttgattc tgcactatct aactgtgtca cgattgcttt cttctcatct tggattgcaa
     5701 cagcttcttt aggcaattct gattctttga tgtcgtaatc atctgctgaa agtttgacag
     5761 aggatagctt atttgacaaa cttgtgattg tttcttctgt tatttcaggg tttagaaaat
     5821 ttttttcttt tgtactatac agcttgtcta cttcttttga taaagaagtc aaggtgtctt
     5881 tttgattggt gatggcagat tctgcataat tgatccgttg ctgatgttgc cgctgattat
     5941 tgatatggta accaaacaaa cagatcgcaa tagcacctgc acctaccgcc acagcagtca
     6001 atgacttgac gaatggcttt tccatagatt cactcctaag tattgatgat agataaatta
     6061 agatataatt cacatacata aaaatgacag ctttttattt taaaaaacat aattaaatta
     6121 atatattcac agaaatattt gtaaacaagt tttccctata taatcatacc acaaactatt
     6181 ttcttgacta gggcaattta cgatttaaaa gtaaataaaa cattaataag ataacaatta
     6241 atcgatttca tttttatttc cttaattttc ataggtgttt ttaaaagtta cacaaaaaat
     6301 aaaataacaa aaaaattaac attgaattaa taatagttat ttatatttat aacaaataaa
     6361 ccagagaaat agcctatttt gtgcctgttc tctggtttat ttacatatta gaaattattt
     6421 tgtgatgatt tgatctaaac ttgctagtga caaagccatg gcagcaattt gacttaattg
     6481 cgttaatcgg ttcttacgta ctgctggttc ttctgccatg accatcgtct gatcaaaata
     6541 attttcgatc agtgggcgaa ggcttacaag ctgatcataa ttttcgttga tcgtttggtc
     6601 agcaaagctt gttttcattg cctctactgc tttgtgcagt tctttctccg cttcgttttc
     6661 gaacagttca gggtctactt ctgattgttt ttcaagctcc gcttttttcg ctaagttcac
     6721 gacacgtgtc aaagcttcca tcgatggttt gaagtcttca tctgcaagat gctgcttgaa
     6781 caattgtgct gctgcaaata cttttgtcag atctttttgt tctgcattca aaacagcttc
     6841 gatgacatca tgacggatat tcttcgttgt caataattgg cgcagacggc ctttgataaa
     6901 gtcgatcacc tgttgttgtc ccgctgacaa gccaatgcca aaacgatccg tatctttact
     6961 gataacttca ctaatcgttt cttgcagaac agacaaagga aaggcccatt ttttcgattc
     7021 gatgattcga acgacaccat atgcttgacg gcgcaaagca tatggatcgt ttgagccact
     7081 tgggatcatc ccaactgcaa agaaagaaaa gatactatct aatttatcag caatcgctaa
     7141 aacagaacct actgtagtct caggtaaatc tccttcactg gatataggca tgtaatgttc
     7201 acggatcgct gttgctacgg ctggtttctc gcctttcagc agagcatatt tttctcccat
     7261 gatcccttgt agttcaggga attctcctac catgttggtt actagatcaa atttatagat
     7321 ttcagcagcg cgttgcagat ccatcaattc ttcctcagat agtcctactt gttcgcctat
     7381 gatgcgagcg atgacttgta cccgttgcat tttctcatag atgctaccga ttttttcatg
     7441 gaaagtaaca gtttttaatt tttcaacgta ttggtcgatt gaagctttct tgtcttcatt
     7501 ataaaagaat tccgcatctt ctaaacgagc cgtcaatact ttttgattgc ctcgtaccac
     7561 attttccaat tttacccgat cgccattacg agcagaaata aaatgaggaa gcaacatacc
     7621 ttgatcgttc cgtacttcga aataacgttg atgttctttc atagaagtga ctagtacttc
     7681 ttcaggaaca gatagatatt tttcttcgaa gtcgccagaa aagacagttg gatattcgac
     7741 taaattgttg acttcttcta gtaaatcttc atctagatca atgatccagt gatttttttc
     7801 ggctaattcg tttgcttgtg tgatgatcat ttcttttctt ttttgcggtg aagcaataac
     7861 aaattgttca ttcaattttt cttcgtattc ttttgcatgc gtaaatgtca catcttcgcc
     7921 taagaaacga tgacctcttg aagctctgtc tgttttgaca tccaagatct caaacgggat
     7981 tatttcgtca tctaacaatg ccacgatcca gtgaataggg cgaatatatt caaaatcgta
     8041 ttctgcccag tgcatcgtta ctgggaaagt gaggctttta atgacatctg ctaaacctgt
     8101 caatacttct tttgcttctt tgccgtgagt gtatttcgtt acatagacat attccacgcc
     8161 ttttaattct ctaaaagtga tagcatctgt cgtcagacct tgtccgcgga caaatccttc
     8221 agctgctttc gtccagtttc catctgcgtc caaagcaatt tttttggctg gtcctttgac
     8281 ttcttcttcg ctgtctgctt gacgttctgg aatgtttgtt actttgattg ctaagcggcg
     8341 cggtgtagaa aacgtttcga tcgtatcata agtcaattgg ttttcatcta aaaattttgc
     8401 tgttttttct tctaattgtt tcatgcttgg tgtgacaaca tgagcaggca tttcttccag
     8461 cccgatttca agtaataagt cttttgccat cttattcagc ctccttcatg ttttctggat
     8521 ttgctgtgtt tttattcaat aaagggaatc ctaatttttc gcgttctgcc acaaagattt
     8581 tggctacagc acgagccatg ttgcggatac gggaaagata acctgctcgt tctgttacgg
     8641 atactgcacc gcgagcatct aataaattga atgtatggct gcatttcaaa atataatcat
     8701 acgctggatg aacaagattt tcatcgatac agcgtttagc ttctttttca aaacggtcaa
     8761 agttgcttag tagcaagtct tggtcactta cttcaaaaga atatttggaa tgttcatatt
     8821 ctggttgttt gaagatctcg ccatatttca caccatcagc ccattccaga tcatagacac
     8881 tttcaacttc ttgaatatat gaagcaagac gttctagtcc ataagtgatc tcagaagtaa
     8941 ctggtttaca aggtagtcct ccgacttgct ggaagtacgt gaattgtgtg atctccatcc
     9001 catcaagcca tacttcccaa cccacaccgg cacaccccat cgatgggttc tcccagttgt
     9061 cttcaacaaa acgaatatcg tgttctaatg gatcaattcc taaaagacgc aagctttcta
     9121 aataaagctc ttggatattt tcaggtgaag gtttcattac gacttgaaat tgatgatgct
     9181 gatacaaacg gtttgggttt tctccataac gcccgtcagc tggtcggcgg gatggctcta
     9241 cataagcagc attccatggt tccggtccga ttgcccgtaa gaatgtatat gggctcatcg
     9301 tacctgctcc tttttctgtg tcataagctt gcatcagcat gcagccgttt gatgaccaaa
     9361 atttttgtaa tgttaaaatc atatcttgga cggtcaattt tttactcatc tgttttgccc
     9421 ctttcaattt ttaagcgttg ctgtcaataa gaatacagga aagaaaacga caaaaagtcc
     9481 ctatgtctga ttacagacat agggacgaat aatttcgcgg ttccacccta cttccgaata
     9541 aagattcggc agcttcattt atcgtactcg caagtgcctc ttcaaaaatc gtttgggtcc
     9601 ggcttccact ctcccgaact cgctaaacca cgtgtttttt actgatcttg gtcaatgtac
     9661 gtattcattt tgcagttttc ttgtttatat aatagatcct ctcagtgaac gatctattta
     9721 atctacggtt aataatagac ggaagtccct gtatttgtca aatagttttg atttttttgt
     9781 tgtatctttc atttattctt ctttttcagg tatctctttt tcaggcatat ttttctctgg
     9841 cgtctttaat acgtgtgccc aagacttcat ttgatcaatg aactttttgc tttttagatg
     9901 aataccgaca tattcatcat acaactggtc aatgaggtca cggattgcct gcttcgtttc
     9961 ttctttcaag tggattccac tgatcttatc ataagaaata gccgaaaaca ttcgaacgaa
    10021 ataaacagcg cgaggatctg catgataacg gtgttcgtcc attggccagt gtttctcgca
    10081 tatcacaccg ccagatttgg aagaatagtc aaattttcct tttgtttccc cgcatacgac
    10141 acagtgcgtc caaattggcg cgattccgaa gcgttgcagt atttgaactt caaagatatt
    10201 cgtgatgatt tctgcatcat ctccttgatc catccgctgc agagcttgtt gcgtaaattg
    10261 gaataaatgc gggtcatatt gatgatcttc gattgctgca tctactagat ttagcaaata
    10321 ggtaccataa ccgctcaaaa agatatcttc ttggatgttc cgaaacgcat ggacgtcttt
    10381 gacactgctt aaaaacgaca aaccttcact tcgaaaatta ccgatataga ctgcttcggt
    10441 aaaggggagc aaagccggag ctaatggctg attttgccga tggattcctt tgatataaaa
    10501 catcattttg ccagaagatt ccgtaaagat tttgactaat ttatcttttt ctttgtaatt
    10561 acggctagat aaaatcaagc ctttcgattc tgcaagctgg ctcatattct ccctcctaaa
    10621 aaataaatct ggaaaagcag attctgccga tccagattat ttatgtttcg atcttccgcc
    10681 gtttttctca acgtgcggat ttttatccag tttatttcta tacttaacct ataagaagtt
    10741 cagaatatca gtattcgtct tttcggtagc caaagtcttg cagatagact tttttgtctc
    10801 gccagtcttt ttggactttg acccaaagtt ctagaaatac tttgtctcct aaaagatttt
    10861 cgatatcctt tcttgctttc gttcctactt gcttcagcat ttttccgcct ttgccgataa
    10921 tgattccttt ttggctatct ctttcgacga tgatcgtggc ctggatatgg attttatcat
    10981 tctcgtttcg cttcatcgaa tcaacaacga ctgcgacaga atgaggcact tcgtctcgtg
    11041 ttaaaaacaa gactttctca cggatcaatt cagaaacgat aaagtattca ggatgatcag
    11101 tcacttgatc atctggaaaa tattgtgggc cctctggcat ttcactgact aatacatcca
    11161 ttaagcgttc gacattgttt ccttctgtag cagagatcgg aatcacttgt gcaaaaggca
    11221 tctgcgtcga ataatcttcg atgattccca gcaactcgtc tggatggatc ttatcgattt
    11281 tattgataat cagataaact ggagaatgca cgttttttag acgttcaata ataaaatcgt
    11341 ctccccggcc gcgtttttga tcagcactga tcataaataa gactgcatcc acttctttca
    11401 acgcactgta agctgcttct accataaaat ctcctagacg atgctttggt ttatggattc
    11461 ctggtgtatc gataaaaatc agctgagctt ctggtgtagt atatactcct tggattttat
    11521 ttctagttgt ctgtgccttg tcactcataa tggcaatttt ttgacccacg attcggttca
    11581 ataatgtgga ttttcctaca ttcgggcggc cgacgattgc gacaaaccct gatttatgtt
    11641 ctggcatata cgttctcctt tttaattaaa caacaaatga tatattttag ggacaaataa
    11701 aatcaaccca atgatgacag caaaacaagc ggtcactaaa actgctccag ctgccatatc
    11761 tttgattttt ttcccaatcg gatgaaaatg aaaatccgta aacatgtcca ctacattttc
    11821 aaaaacagta ttgatgatct ctactatcca aacgagaaaa acagctaaaa gcagccataa
    11881 ccactctatc cgatcaagct ggaaaatcac gcccataaat aaagcaagga taccgaacgc
    11941 aacatgtttc ctcatattgc gttcttcctc aaaaacggtt ttgactcctt ggacagcaaa
    12001 ttccacagac atcaggaaat gtttgttttt ctctacgttt ttatcttttg agtccatagg
    12061 catccaagat ctctttctgt aaaccaaaca tttctttctc atcttctggt gtcatatggt
    12121 catacccgtt gatatgcaag aatccatgta ccgctaaaaa gccaagttcc cgatcatatg
    12181 aatggccgta agcttctgct tgttcagcag cacgttcaat agaaatcatg atgtctccta
    12241 gtacacgagg caaaggattc tctccatctt caaaaatcac tgggatttcg tcttcgcctt
    12301 cttcttccag agcaaaactg atgacgtctg tcggtgcatc ttttccacgg taatcacgat
    12361 tgattacttg gattgccgca ttgtccatga aagtaacaga catttctgtt tcctctggca
    12421 gctttaaata agatgctgca aattgcagca agtcatcgat ttctttcatt ttctcttctg
    12481 agacttggtt tgtttcatcc ataaaggtaa tatccattat acatcgcttc tttctattct
    12541 tcttgttctt cctttatctt ctcaacttct gttttcattt taggatattt gatccgagaa
    12601 tggaaggtgc tacttaaacc gctgatcaat gatttttcca cttttcggat ttctttcaag
    12661 gtcagtccac tatcgtctaa ttgaccatcg gaaatacgct cttcgataag attatggaca
    12721 aaactttcga ttttttcgct cgtcggatga tccattgccc gtactgcagc ctcacaactg
    12781 tcagcaatat tgacgatccc tgcttcacga ctttgcggtt ttggtccagg ataacggaat
    12841 tgctcttctg ttacttccgg attgcgttct ttcgctttga tgtagaaata acgcattagc
    12901 gtggttccat gatgctgttt gcaaatatcg atcaccattt gcggcatatt ataatcttcc
    12961 aatattttgg ctccatctgt cacatgtcca aagataatat gtttactgtc ttctggcaat
    13021 aagaaattgt gcgggttttc cgctcctgaa ggcaagttct caacgaaaaa gctagcatgt
    13081 ttgatcttgc cgatatcatg ataatagcaa gctactcgag ttagcaaaga acgtccgccg
    13141 atttctgcta cagcgttagc acttagatta gctaccatca tactatgatg ataagtacct
    13201 ggcgcttctt ccagcaactg tttcaataaa ggatgatttg gattgctgag ttcatttaat
    13261 acaattacac tgtcatcatt gaccatcaat tcaatatatg ggtgtagacc gatcgtcagc
    13321 aagaatgaga agagtgcacc agtgaacccg cagacaagca ttaaccaagt cgttgcatca
    13381 ccaaagctca ttccttgata gacgatcaaa atgacatcca tcatcacagg aaaaataatg
    13441 acccacatgg cagcagataa tccttgttct gagatccgtt tgcgtctgac aatcgttgcc
    13501 atgagcccag agaaaacata agacatcaag ataatcgtta gaaagttcgt gccaatcgaa
    13561 tcataaaaaa tgaaaagtgc agatactgct tggaaaatac cagccatgat ccctgctctt
    13621 cggttcaaga aaaagcttaa aacaagtggt gcaaaagcag cgggataaaa cagcgggata
    13681 taagtagctt gttccgtttg gaataattgg aaaaatttca tcagcactat gctgactgac
    13741 atagctgtca catagaaaag cacgtatttt gttctttctc ctgcattttg gaattgaagt
    13801 gaattataaa tcagtacgcc gatttgaagc aaaacagcta aaaccatggc aatgagtggg
    13861 aaaatcgaag tcgtttgatt cgtcaatcct agtaattcca attttttcat cgctgaagca
    13921 tcaatttgat ttccttcgcg aacaatgatt tctccttgga agatcatcac tggttgtaca
    13981 gcttctcgtg cagattgttt taattcttct gtctttttct cattcaatac atcattgact
    14041 acaatgcctt gatccactaa gtagcgaatc atttgttgct gctctgttgt gacattcaaa
    14101 tactgaatct gatcttctgc ttcttgttta aaagcttcca attcgctttc acgaacttgt
    14161 ttactcatct gttcgtcaat gagcttcaaa ctttcatccc taaccgtgtg aatttccgtt
    14221 tcagtcattt caaatatcgt cgaataaaaa ttgttcggca gtttttggta aaacgcaacg
    14281 ttttctgcat tcacgttttc aaaatttttc ttcagtgtag caatccgctc ttctgtagta
    14341 ggtttcggga tattttcatc ttccttcgcc ttatctacct tgtcattata agatttgttg
    14401 atggtatcat tcgtcttatc gatcaattcg aataattgct tgatccgatt gttctggtca
    14461 tctgccagat ctttttgata agtatattct ggagtcactg cttctgctgc cagttttctt
    14521 ctttgttcgg tttcttccgt gttctcgatc gttttattcg cacggatact ctcttctgct
    14581 acttggcctt ctctgaaatc attggccttt tgtttgacac tactaaatgt aacgccgcag
    14641 acaatgataa aaaacaagaa cagaaggaaa ggcaatgctg cctttccgag tttttcttgt
    14701 agtttctgca gtgttttatt caacatgtgc ttatcctcct aagcaaagct tattcttgat
    14761 gaagatccgc cttttcataa gctttgatga tttcggcaac tacagggtgc cgaacaacgt
    14821 cacttgcttc aaaattaaca aaatcgattt ttttgatttg ctttagtgtt cgttctgcat
    14881 gaacaagacc acttgtcgtc cctcgaggca agtctatctg acttgtatcc ccattgacaa
    14941 tcatttttga ttgataaccg agacgggtca agaacatttt catctgagcg actgtcgtgt
    15001 tttgtgcttc gtccaagata acgaatgcat catccaatgt ccgcccccgc atgtaagcta
    15061 atggtgcgat ttcgattacg cctcgttcca tcaatcggtt cgtatgatcc aatccaaaaa
    15121 tttggtataa agcatcataa acaggccgca gataaggatc cactttttct ttcagatccc
    15181 cgggtagaaa gcctaggttt tcccctgcct ctacagcggg ccgcgtcaaa atgatttttt
    15241 gcacttcgcc tttttttagg gcagcaattg ctaatactac ggcaagaaac gttttccctg
    15301 ttccggctgg ccctacacca aatacgatgt catgtttggc taccgattca acgtatttct
    15361 tttgaccgct gttttttacc cgtataggtt ttccattacg gtctttgacg atctcttcct
    15421 catacatctc aacaaaataa tctaatgtac ctttttgccc cattttcaat gccgtcacga
    15481 catctggtgt acttacgtgg attcctcgtt tgatcaattc ctgtaatgtc cgaatgactg
    15541 aggcagccaa ggaaatctgg gattgttcac caatgatctg aatgatttct ccacgtgtat
    15601 ggatggttgt ttccgttgtt tcctcgatca gttttatatg cttgtcgtgt gcacctaaaa
    15661 gcatgctgac atcatcagca gctgtcaatc taatctctaa agaactagac tcttccgtca
    15721 aaaatagaca tccccttttt ttcgattcat tctttcctat tttaccatac tcagaagaaa
    15781 ctatatagca aataagaaaa ggttgtaaaa gagtcgttta accatacgta ccaagaaacg
    15841 ttaaaaaata gctgtttcca ttcgctaaag gtctcgtttt gataaaaaga atctgagaat
    15901 gtaagttata tgaaagtgga gaacaaaat
//
LOCUS       DAYYGO010000065        15255 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000065 DAYYGO010000000
VERSION     DAYYGO010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.067"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            333..947
                     /gene="gmk"
                     /locus_tag="ACHA5T_001755"
     CDS             333..947
                     /gene="gmk"
                     /locus_tag="ACHA5T_001755"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFM8887325.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            952..1257
                     /gene="rpoZ"
                     /locus_tag="ACHA5T_001756"
     CDS             952..1257
                     /gene="rpoZ"
                     /locus_tag="ACHA5T_001756"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM8887326.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            1510..3918
                     /gene="priA"
                     /locus_tag="ACHA5T_001757"
     CDS             1510..3918
                     /gene="priA"
                     /locus_tag="ACHA5T_001757"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFM8887327.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            3933..4424
                     /gene="def"
                     /locus_tag="ACHA5T_001758"
     CDS             3933..4424
                     /gene="def"
                     /locus_tag="ACHA5T_001758"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFM8887328.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            4417..5355
                     /gene="fmt"
                     /locus_tag="ACHA5T_001759"
     CDS             4417..5355
                     /gene="fmt"
                     /locus_tag="ACHA5T_001759"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFM8887329.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            5355..6713
                     /gene="rsmB"
                     /locus_tag="ACHA5T_001760"
     CDS             5355..6713
                     /gene="rsmB"
                     /locus_tag="ACHA5T_001760"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFM8887330.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            6726..7466
                     /locus_tag="ACHA5T_001761"
     CDS             6726..7466
                     /locus_tag="ACHA5T_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFM8887331.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            7463..9532
                     /gene="pknB"
                     /locus_tag="ACHA5T_001762"
     CDS             7463..9532
                     /gene="pknB"
                     /locus_tag="ACHA5T_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFM8887332.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            9697..10596
                     /gene="rsgA"
                     /locus_tag="ACHA5T_001763"
     CDS             9697..10596
                     /gene="rsgA"
                     /locus_tag="ACHA5T_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFM8887333.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            10609..11259
                     /gene="rpe"
                     /locus_tag="ACHA5T_001764"
     CDS             10609..11259
                     /gene="rpe"
                     /locus_tag="ACHA5T_001764"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFM8887334.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            11260..11898
                     /locus_tag="ACHA5T_001765"
     CDS             11260..11898
                     /locus_tag="ACHA5T_001765"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFM8887335.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            12053..12907
                     /gene="mreC"
                     /locus_tag="ACHA5T_001766"
     CDS             12053..12907
                     /gene="mreC"
                     /locus_tag="ACHA5T_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFM8887336.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            12911..13420
                     /gene="mreD"
                     /locus_tag="ACHA5T_001767"
     CDS             12911..13420
                     /gene="mreD"
                     /locus_tag="ACHA5T_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFM8887337.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            13675..15249
                     /locus_tag="ACHA5T_001768"
     CDS             13675..15249
                     /locus_tag="ACHA5T_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFM8887338.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
BASE COUNT         5114 a         2864 c         3319 g         3958 t
ORIGIN      
        1 gtattaagcc aaatttatgc aaaaatgtga gaaactattt tcacaaaatt tttcttcata
       61 ctcgaaacta atcggcttct tcacgacctc ttatacacgg cgttcaaaag cttgcatctg
      121 cgataataac cccaaccaaa cgaaaaacat gagaagctgt ttatgtttgg ctgttcttat
      181 tacttgatgc aggacagctt ttttacgacc tcttggtaca gggtagcttt tttaccaatt
      241 tctagctata attaaagata accttgaaag ttgcagaaaa aaagtgcaaa ataaacgata
      301 agtacaaaaa gactagttga aaggacgtcg tcatgtcaga gcgtggatta ttaatcgtgt
      361 tgtctggtcc atccggtgta ggaaaaggca cagtacgaaa agcaattttt gagagtgagg
      421 ataatgattt tcagtattcg atctcaatga ctacccgtaa aatgcgtgaa ggggaggtcg
      481 aaggcgttga ttatttcttc cgaagcaaag aagagtttga atcaatgatc gaagctggag
      541 agatgctgga atacgcagaa tatgtcggga attattatgg aactcctttg tcatatatca
      601 acaagacatt ggatgaagga aaagatgttt tcttggagat tgaagtccaa ggagcaaaac
      661 aagtcaaaga aaaagtgccc gatggtgtat tcatcttttt gactccgcca gatttagcgg
      721 aattaaaatc acggatcgta ggccgaggaa cagatgcaga tgaagtaatc gaagaacgaa
      781 tgaaagttgc aaaagaagaa atcgaaatga tggctcttta cgactatgct gttgtaaatg
      841 atgaagtgcc gctagcagtt gaacgaatca aaaacattat tgccagtgaa cattttcgag
      901 tagatcgcgt aatcggtaaa tatattaaaa tgttaaagga gatgtagtct tatgatgtta
      961 aaaccttcaa ttgactcact attagatcgt gtaaattcaa aatattctct agtgatttta
     1021 gcaagtaaac gcgcgcatga attggatgca agtgcacagc ctactctaga ttcttttgat
     1081 tcagtaaaaa gcgtcggtca agctttagaa gaaatcgatg caggaaatgt agtcaatgat
     1141 ccacatcctg aattaaaacg tgaacgtttg aagatggaag aagaggaacg tcaagcacaa
     1201 aaagagcgtg aacaacacga attggaatca cgtatccgtg aagagcaaaa aatgtaataa
     1261 agaaaaatag agcgaacatc taaaaagaaa agcaaaccca gctgtttcct tttcaaacat
     1321 tcgccactta ttgtctggta gaaaaggctg tgtctgaact ttttgacaca gttctttttt
     1381 ttcttatctg ctgttaattt tttgttagga tttggcagaa gataagacaa atccatcgat
     1441 ctctagtaaa ataggcaagg aactttttgt cgcttgataa gaaaagacaa aaaaaggagg
     1501 aaacgaacca tgacattaac agctgaagtc attgtggacg tacctactat gcagaccgat
     1561 cagccattca cttatttggt tccctctgaa atggaaacag ctttgcaagt cgggatgcga
     1621 gtagaagtcc cttttggtaa tggcaatcgt catgtccaag gatttgttat ggctatccaa
     1681 aaatcagaag aatcagctaa tccatctttg aaggcagtta ttcgtttgct agaccttgct
     1741 ccggtggtca atgaagaatt actttcatta gctgattata tgaaaaaaat tacttacgca
     1801 ttcaaaatca cttgtctaca aacgatgctt ccgagtgtga tgaaagcaga atatgataaa
     1861 ttgatctatc ctttggcaga cacaccagaa gttcaggtat tgtttgatca gcgagaagtg
     1921 atttcctgga aggaagcaga agaagaaggc agcctttctc aattgattcg ctggcgccaa
     1981 gagcaattag tagatatcaa atatgaagtc catacacgaa ataaagtaaa aacgattcgt
     2041 ttggtccgtt ctttgttaac tgaaaagcaa atcgaagaag aatgggcaaa attacggcaa
     2101 aatgccaaga aacaaaaaga acttcttttg tgtttatctg aactgagcca agaagaacca
     2161 attgcttatt tcaaaaataa aggaatcagt actgctgtct tgaatcaagg aaaagaaaag
     2221 ggctggctgg agtttataga atcagaacgt tatcgcgacc cttataaaga ccgagtattt
     2281 gaccaaacga cggcattgga actgaatgca gaacaaaaaa atgctgtcga acaaatcatc
     2341 acagcaggtc agcagcaaaa agatcaggtc tttttgctgg agggaatcac tggcagcggg
     2401 aaaacagaag tatatcttca ggcaatcgct gatgttttgt cagaaaataa aacggctatt
     2461 atgttagtgc ctgaaatcgc attgactccg caaatggtcg agcgattcaa aggacgcttc
     2521 ggagaatcag ttgcggtctt acatagcgga ctttcccaag gtgaaaaata tgatgaatgg
     2581 cgcaagattg agcgggaaga agcgcaagta gtagtaggcg cacgatctgc tatttttgcc
     2641 ccgctgaaaa atatcgggct gatcattatc gatgaagaac atgaatcaag ttacaaacaa
     2701 gatgagacac cgcgttatca tgcgagagat ctagcaattt ggcgcagtaa atatcatcat
     2761 tgcccaatag ttctaggaag cgcgacgccc tctttagaat caagagcacg agctcaaaaa
     2821 ggcgtttatc agttattgca gttaaaccac cgagccaaag cagctgcaca gcttccagct
     2881 attgagatcg tagatatgcg tgaagagttt cagaatcatc ggacctctac tttttctgct
     2941 aatttgcagg aaaaaataca aaatcgatta gacaaaaaag aacaaactgt cctactgctc
     3001 aatcgtcgag gatattcgtc ttttgtgatg tgtagggact gtggatttgt tcttccatgt
     3061 cctaattgcg atatctctct tacactgcat atggatacac gttcaatgcg ctgccattat
     3121 tgcggacatg aagaacctat tcccaatcgc tgtcctaatt gtggcggcaa taaaatccgc
     3181 tactatggaa caggtactca aaaagtagaa gaggaactcc gagaacttta tccgcaagct
     3241 aggattttgc gtatggatgt agataccact cgaagaaaag gggcacacga acagatttta
     3301 caaaaattcg gtgcaaaaga agcagatata ttattaggga cacaaatgat tgctaaaggt
     3361 ttggactttc cagaagtgac attagttggg gtgttgaatg ctgatacctc cctgaattta
     3421 ccggattttc gttctagtga gcatacattc caattgctta cccaagtttc aggacgagca
     3481 gggcgagcgg aaaaagcagg cgaagtcgtg atccaaacat tcaatcctca acattacgcc
     3541 atagaattag ctaaaaaaca aaattatgag cagttttatc agcaggaaat gcatgtacgg
     3601 catcgtggag gctatcctcc ttattacttt actgtgaaaa taaccgccag ccatcctgaa
     3661 gaacaagtag cggcaaagaa aatctttcag attgccaatc agctaaaaga agtgttaagc
     3721 cctcaaagct tgctattagg gccaacgccc agcatgattt tgagagtgaa aaaccgatat
     3781 tattatcaac taatcatcaa atataaacat gaaccgaatc ttcctcatgt tttggatgaa
     3841 attttgaatg gaagccagaa agaacagcga caaggtcttt ttgtggcaat tgacaacgaa
     3901 ccattaaact ttatttaaga aagggccaaa ttatgcgcta tcctatattg atccacccta
     3961 atgataaatt aaaaagaact gctcagccaa tcgatgtaat cacggacgag acaatcgctt
     4021 tattagacaa tttatatgag acaatgattg cgaatgacgg aatagggatc gctgctcctc
     4081 aagtaggaca aaacaagaga attgctgtga tcgaagtaga tgaaggcgag aaatttgaac
     4141 tgattaaccc agaaatcatc gaagcaaaag gtgaaagcct tgacgtagaa ggttgtctca
     4201 gtattccgca tgtctatgga acggtcaagc gagcagatga agtgaccgtg cgttattatg
     4261 atcgagacgg agaagagata gaagtgacgg cttttggcta tttagcccgt gccttccagc
     4321 atgaaatcga tcacttagac ggtatcttgt tcattgagaa aatgatacag cagatcccgg
     4381 aagaagaact tgaagaatat atggaggaac atcttgatga ctaagctagt atttatgggc
     4441 acacctgctt tttctgtgcc gattcttgaa gggctgatag aatcaggtta cgagatccag
     4501 gcagtggtaa cacagcccga ccgtccagtt gggcggaaac gagtaattac gccgacaccg
     4561 gtaaaagaag cggctctgaa gcacggtatc cgtgtgctgc agccagagaa aatctccggt
     4621 tcgccagaaa tggaagaaat cattgaattg gtgcctgatg tgatcgtcac tgcggcattt
     4681 ggccaattct tgcctgaaaa gttattgcaa gtaccaaaat taggggcaat caatgtccat
     4741 gcttctttac ttcctaaata tcggggaggt gcgcctgttc attatgccat catgaatggg
     4801 gagaaagaaa ctggtgtaac gatcatggaa atgatcaaaa aaatggatgc aggcggaatc
     4861 tatgcgcaag aaagcatacc aatcactaaa caagatgatg tagggacgat gtttgaaaag
     4921 cttagccttt taggaagaaa gctattgcta gaaacattgc ccaatatttt ggatggacaa
     4981 aaaccagtac ctcaagacga gtcagaagca actttttcgc caaacatcac acgggaacaa
     5041 gaagcaatcg attggcataa aactgcagaa gaaattgaca accaagtacg cggaatgcgt
     5101 ccttggccaa ttgcattcac gacctatgag ggcatacgct ggaaattatt atcggttgaa
     5161 gtattggcag aaactacaga caaagcacct ggtacgatca tccggaaaga caaaaaaaat
     5221 ctatggattg cttgcggtaa acagaccgtc ttagccatca atgaattaca gccagctggt
     5281 aaaggaaaac aggccgtaca tgatttcctt aacggaagcg gtcagcaagt tcagattggt
     5341 cagcaggtga aataatgaaa aaaatcccta aaaaaatgaa aaaatcagta cgatatatgg
     5401 cattactgac attagagcga gtgaacaagg gtggcgccta ctcgaatctt ttgttgaatg
     5461 agatgatcaa acaaagcgag ctaaatcaaa aagatgtggg tttatttaca gaattgatct
     5521 atggtacgat tagccatcag cgactattgg aattttacgt tgcaccattg atcgctaaag
     5581 caaaaaaagt ggatgactgg gtcaagacgc ttctttatct ttctgtatac caattagaat
     5641 ttttagataa agtccctgca catgctattt taaacgaggc ggtcgaaatc gcaaaagtaa
     5701 aaggaaatcc tgggacaggg aaatttgtaa atggtgtctt gaggaattat cagcgtcaag
     5761 gagcaccaga tctgcaaggc attgctgatc ctatcgaaag actttctgtc aaaatcagcc
     5821 tgcctctatg gatgacgaaa cgactggtgg atcaaatcgg atacgaagaa actgaaaagc
     5881 tcggtttatc gctatatcag cctagccatg ctagtgcccg agtagataca cgccgacttt
     5941 ccagagatca ggccatcact gttttgcaag aagaagagat cacagctgaa aaaagccaag
     6001 tcagtccata cggtatagta gcgaaaaaag ggcatttagc ttctagctca ttgttccacg
     6061 atggcgtcat gacgatccaa gatgaaagtt cgatgcttgt agcgccgtct atgcaaatcg
     6121 agcctgaaca tcatgtctta gatgcctgtg cagcacccgg aggtaagacg acgcatattg
     6181 cttctttttt agatccgtca tgcggtggag aagtggtcgc tttagatgta cacgaacaca
     6241 aagtcaaact cattgaagat aatgcagcac gtctaggtgt ctcttctgct gtctacgctc
     6301 aaaagatgga tgctagagaa gtcagtaaac agttcgaagc agagcagttt gatcggattt
     6361 tagtagacgc gccttgttca ggtcttgggc tgcttcggcg aaaaccagat attcgttata
     6421 aaaaacagcc ggatgaatta gcgaatttac caaaaatcca attggaaatt ttagaaagct
     6481 gcgcaccaac tttaaagtct tccggtattc tgacttatag cacttgtacg atcttaaaag
     6541 aagaaaacca agacgttgtt tctgctttct tggaaagaca ccctgatttt gagaggattg
     6601 atgttttgac caatcctatg ctacaatcat caatcgaaga gaaaatgttg actttgtatc
     6661 cgcatcagtt ttacacagat ggatttttca tttgttgttt gcgcaaaaag taatgaggtg
     6721 aaaaaatgca gattgaatat caatcagatg taggaagacg acgtaacaca aatcaagact
     6781 atgccagtgt atttaccagc cgttcaggtc tcaagcttgc tgtattagca gacgggatgg
     6841 gtggtcatcg tgccggagac attgccagtc agatggcggt aacgaatcta ggcgaagctt
     6901 gggaaaaaca agaagctgtt gacgatgaaa aaatcgccca atggttcatt caatcgattc
     6961 aagaagaaaa tatgctgatt tatcagcgtg gacaagaaca gccggaatat aacggtatgg
     7021 gaacaacaat cgtagcggcc gctctgtcag atgactggtt cacgattgcc aatgttgggg
     7081 atagcagagc ttatttgatt cgtgaagata aaatcatgca attgacggag gatcattctt
     7141 tagtcaatga actagtcaaa tcaggagaaa tcagcgaaga aatggcagtg aaccatccaa
     7201 ggaaaaatat tttaacacgt tctgtcggca tgcctggaac agtcgaagta gatgtttcta
     7261 ctcatctttg gcaactaaaa gatcgactgc tgttatgttc agatggcttg acgaacatgc
     7321 tgtctgaaga agaaatagga acgatcatca atcaagaagg cccattgtct cataaagtaa
     7381 ctgagctgat tgatcgtgcc aatgaagcag gcggagcgga taatatcacc gttcttctta
     7441 tcgaatataa ggaggacagt gtatgatcga gttgggcaaa aaattaaatg gccgttatca
     7501 aatcactgga aatatcggta gcggtggtat ggcaaatgta tttttggcgc acgatttaat
     7561 cttggatcgt gatgtagcag ttaaagtctt acgatttgac tttcaaaatg accagactgc
     7621 gattcgacga tttcagagag aagctttagc agcaacagaa ctcgttcatc caaacattgt
     7681 tagtgtttac gatgttggtg aagaagacaa catgcagtat ctggtgatgg aatacgtcaa
     7741 aggaatggac ttgaaacgat atatccaaac acattatcct gttccttatg aaacagcagt
     7801 aaatattatg cagcagatcc tttctgctat ttctttagct cataaccatc aaatcatcca
     7861 ccgagatttg aaaccccaaa acgtactgat cgataatgaa ggtgttgtaa aaatcacgga
     7921 tttcgggatt gctatcgctt tatctgaaac atcgatcacg cagacgaata cgatgctggg
     7981 atctgttcat tatctgtccc ctgagcaggc cagaggaagt atggcaacga agcagtcaga
     8041 tatttatgct ttagggatca tcctttatga gatgttgacg ggtagcgtac cgtttgatgg
     8101 tgaatctgct gtaacaattg ctttaaaaca ttttcaagat gatcttccat ctataaaagc
     8161 acttgatcca aatgtgccac aagcactgga aaacgtgatt ttacgggcaa cggctaaaga
     8221 acctgcagac cgttataagt cagccgagga aatgtctgat gatctttcta cagtattatc
     8281 accagctcgt gcaaacgaag aaaaatggca gcctcatgtg atggataatg aaacaaaagt
     8341 tatcacaccc cttacggaag atgcaccaat gcctgattca ttcaaatcta tgcctctgcc
     8401 aaaagacaag ccagaaaacg aaaccttgcc agaggaggaa gaagttctgc ctgatcctaa
     8461 aaagaaagga cagaaaaaat ggtggctgat cctattagct attcttgctg ttttaggaat
     8521 cggaacagct gtattctttg cttctggcgg gcgaggagaa gttgttgttc ctgatgtttc
     8581 tgatctttct gaagcttctg ctagagaaac actcgcaaaa gcgaaattag aagtagcaac
     8641 aaaaacagaa gaaattgcag atgacaaaat cgaagaaggt aatgtagtga agacagaccc
     8701 ggcagcggga acgacagtca agcaaaaacg cgaagtcact ctgtatatta gtagcggcac
     8761 gaaaaaaatc aaattagatg attacagtgg taaaagttat gaagatgccg aagcaagttt
     8821 gaaagaatta gggttttctg ctgacttgat tcaaaaagaa gaggaattca gttcagaggt
     8881 agaaagcgga atgatcattt cgcaatctcc atcagaagat acagaagtcg atcctaaaac
     8941 agacacgatt actttcacag tcagcaaagg accagaaaca gtgactttga gtagtcatgt
     9001 aggagaaaac tacgaaactg ttcgtaattg gcttgttaca caaggattca atatcaataa
     9061 tattagtgct acttatgact atagcgacac tgtaccatca ggttcgatca tcagtcagga
     9121 tccagcctcg ggtgaagttg tagcagaaga aacttacatc aattttgtcg taagccaagg
     9181 aacagagcct aaattgaatg atatctccgg ctatacaaaa agcgaagcac aaagttattt
     9241 agctagtgtc ggtgcagaat atatcgggca tgagacttcc gaatattcaa acacggtgga
     9301 taaagataaa gtgattcgta cagtaccagg ggcaggaacg acaattacca aaggaatggt
     9361 tgtgaacgta atttactcta aaggaccaga tccagcgtct tctagttctg aaaaagactc
     9421 ttcagacagt tctagcagct caagtagctc cagtagcaca agcaactctt caaaagaaga
     9481 atcttccagc tccacaagtt catcagctag cacctcagaa agttcaaaat aagtgctgaa
     9541 taaaatatat cagaagggaa tgcgacattg atttgttgca ttctcttttt tgtgtttcta
     9601 tctcttttga tcaattttca agccatatac tttgagaagt ttgcatggaa taacacctac
     9661 tttcctcttc gtatggtaaa ataaacaaaa acggaggtgg cacttatcaa aggacaaatc
     9721 agaaaagcaa tcagcggttt ttactatgta tacgcaaacg gagagaccta tcaaacaaga
     9781 ggaagaggaa atttccggaa tcggaaaatc acgccgcttg tcggagatca ggtgatcttt
     9841 gagagtgaaa atcaaacaga cggctatctt ctggaaatct tgccgagaaa aaatcaatta
     9901 gttcgcccac cagtcacaaa tgttgatctt ggaatcattg tcacaagtat cgtagagcct
     9961 gcattttctt ataatctact tgaccgcttt ctagttacct tggaatacga gtcgatcgaa
    10021 ccgattattt ttttgacaaa gaccgacttg tcagacgacc cagaaaaaat cgaagagatc
    10081 aagcaaacct atactaaaat aggctatccg gtgattgtac ccaatcatac gggtgatacg
    10141 acagaactga tccgttactt cccagaacgg ctatcggtct ttatgggtca gtctggagca
    10201 ggaaaatcga cgctactgaa tcaaatatca ccggaactga atttagcaac aggagagatt
    10261 tctgatgcac ttggaagagg acggcataca acacgtcatg tggaactgat tcctatctat
    10321 ggcgggttag tagcagatac accaggattt agctcgattg actttttgac aattgaagca
    10381 ggagaactgc cgagacaatt tcctgaattt gtagaagctt ctgtccattg caaatttaga
    10441 gagtgcatgc atgctaaaga acctggctgc gaagtaaaac atagagtaga aacaggtggt
    10501 atcgcacaga cacgttacga caattatttg caattcttac aagaaatcga aaaaagaaaa
    10561 ccaatgtatg caaaaaaaga aggtaaacga aaataggagg aaacaagaat gaagatagca
    10621 ccatccattt tgagtgcaga ttttgccaat ttacaaagag acattgagtt agtggaaaaa
    10681 ggaggcgcag attacatcca cgtagatgta atggatggcc aatttgtccc aaatatcaca
    10741 ttagggccaa atattgttca ggcaatccgt cctgttacta aattaccgtt agacgtccat
    10801 ctgatgatcg ttgatccaga aaactacatt ccagcatttg caaaagctgg agcagatatc
    10861 attactgtcc acgtcgaagc aactcctcat attcatcggg cacttcaaat gacaaaaagc
    10921 ttaggagtta aagctggagt agttatcaat ccaggaacac cagtctccat gatcaaacat
    10981 gttttgcctt tggctgatca agtactagtc atgactgtca atcctgggtt cggcggacaa
    11041 agctttattg aagaaacagt agaaaaaatt acggaactcg ctgatctacg tagagagaac
    11101 aattggactt atgcaatcga agtagatggc ggaatcgttc cagaaacagc aaaaatctgt
    11161 aaagatgcag gggcggatgt cttcgttgca ggatcatata tctatggagc cgaaaatcca
    11221 gtcggacaaa tcgaacgatt aaaagaggca ttagactaaa tgaaaatttt attagctgct
    11281 ggaggtaatc cgcaaaactg gccgcagtct ctgctgtcca aatacgatgc ctatgtcggg
    11341 atagaccgtg gtgcgctgta cctgcttcaa gcaggctacc cagtagatct ggcagtgggc
    11401 gattttgatt cattgtcgaa cgaagagcag aaggaagtat ttgcaaaagc caaagagtat
    11461 acccgttccc ctgcagaaaa agacgataca gatacacagt taggtatctt aaaaacattt
    11521 gaacgttacc cgcaagcgga agtcactttg attggagcta cgggcggaag attggatcat
    11581 cttttagcca atctctggtt aggtatggaa cctcgcttca aaccctttgc ccatcagctt
    11641 attatcagag attgtcaaaa ttatttgtct tattatttgc ctggagagca tatagtaaga
    11701 cagataccag atatgaagta tttagcttat tgctgcttga ctcccgtctc gaatcttaca
    11761 ttaaaaggca gcaagtatgt attggaaaat aaagaagtag aatatccgac atcttatgca
    11821 agtaacgaat ttctttcaga taccgcagaa ttttcttttt cagatggtat gattgcagtt
    11881 attcaaagca aagattgatc cataaacaaa taaaaaaatt tgtctttcag gctaaaaatg
    11941 acagaatcta ttatttgcta aaaaaaagct taagaggagc aacttacctt ttgtttcacg
    12001 gcatttagtg ataaaattga acacaagagt ctaattaatt cgaggtgtct ttgtgaagaa
    12061 gttcaatcct aataaaaata ttattattac cttggttatt gttatcatcg tggtgacgat
    12121 actaagtgtg accgctgcgc agcgagcgaa tgaaggaaag cccaatttct tccaatccat
    12181 cgttaatgac agtgttgcaa tggttgataa aacaatctct gccccagtac ggtgggtacg
    12241 aaatggagta gattctgtcc ataatctttt ttcaacttat gccgaaaatg agcggctaaa
    12301 agaaaaaatc gatagctatg atgacgtttc acagcaaaac aagaattacg agagagaaat
    12361 cgaagcgttg aaaaaagaac ttgagttgaa tcagacgctg actagttacg aaaaagtaac
    12421 ggcaaatgtc attactcgtt cgcctgacag ttggcaggac atgctgatcg tagacaaggg
    12481 ttctaaagac ggtatcgaag caaatatggc agtcatgtcg caaaaaggat tgattggccg
    12541 agtgatcgag gtcaatacgg cttcgtctaa aatcgaatta ctgtcatcct ctaatgaaag
    12601 ctccaatcat tttccagtac gggtatcttc ggctaatggc gaagcgtttg gtttgcttaa
    12661 aaactatgat gaaaagctcc atgccttagt ggtgacccaa ttaactggtg atacggatat
    12721 caaagaaggg gatgttgtcc agacatccgg tcttggaggg aattctccag ctaacttgcc
    12781 gatcggtacg gttattaaaa cgaaaccaga tagttatggg ctggatcggg aagtttatgt
    12841 gaaaccttat gcagaaatgt atgacgtgtc agttgtgacg attgtccaac ggctagcgga
    12901 ggattagtcc atgttgaaga aagaaacaat gaagtactat ctgccaatcg ttttgttctt
    12961 tttgatgttg atagatggtc atttaacaag aatgctaggg gagtggtcga aaggcaccta
    13021 tatgtcaaat gcccactttc tgatattggc attattatgt tgcagtatgg cgtttgaaaa
    13081 acgttattta ctgattacca cgattgttct cggggctatc tatgacgctt actatattgg
    13141 cgttatcggt atctatgcag tagctctccc tttaattgta tggttgatgt atgtaatgaa
    13201 agacgttatc catgtcaaca tctttactga atttttcagt atgatcatct ttgtcacggg
    13261 ttatgaattg tttacgatgg tggtccagtt gatttttaaa ttagcagtag taaataacac
    13321 gtattttatt acaaggtttt taggacctac actgctgttg aacatgatta tatttgtatt
    13381 attcattttt ccctttaaga aattattcag tgatgaataa tttctttttt tatttttagg
    13441 tcgaaagcga aaaagaatcg ttgttatctt aagaaatgct agacttaacg cggctttttc
    13501 atgatcattt tctgaaacga acagttttgt catttttaaa agttgttttg aaacattttt
    13561 gtaacataac tattatatcg ttgacacact gctatggtac aatttttata tcgttaagaa
    13621 agacttaaat atatttagat atttattaaa ttaagccagt cggaggaatg aagagtgaaa
    13681 aagagtttaa tatcagcagt aatggtatgt tcaatgacct tgactgccgt agcatcaccg
    13741 atcgctgccg cggcggacga ttttgattct cagatacaac agcaagatca aaagattgca
    13801 gacttgaaaa atcaacaagc agatgcacaa tcacaaatcg atgccttaga aagccaagtt
    13861 tctgaaatca acacacaagc acaagaccta ttagcaaaac aagatacatt gcgtcaagaa
    13921 tctgcacaat tagtaaaaga cattgcagat ttacaagaac gtattgaaaa acgtgaagat
    13981 acaatccaaa aacaagcacg cgaagcacaa gtaagcaaca caagctcaaa ctacatcgat
    14041 gctgtattga atgctgattc attagcagat gctattggac gcgttcaagc aatgacaacg
    14101 atggtaaaag caaacaacga cttgatggaa caacaaaaac aagataaaaa agctgtagaa
    14161 gacaaaaaag ctgaaaacga tgctaaacta aaagaattag ctgaaaacca agctgctttg
    14221 gaatctcaaa aaggcgatct tttatctaaa caagctgatt tgaacgtact gaaaacttca
    14281 ttagctgctg aacaagcaac tgctgaagac aagaaagctg acttgaaccg tcaaaaagca
    14341 gaagctgaag cagagcaagc tcgtattcgt gaacaacaac gtctagcaga acaagctcgt
    14401 caacaagctg cacaagaaaa agctgaaaaa gaagcacgcg aacaagcaga ggctgaagca
    14461 caagctactc aagcttcatc tactgcacaa agctcagcaa cagaagaatc atcagctaca
    14521 caatcttcaa tgactgaaga atcatcatca gctacacaat cttcagcaac agaagaatca
    14581 acaacacctg aatcttcaac agaagaatca acagcgcctg aatcttcagc aacagaagaa
    14641 tcaacaacag cgcctgaatc ttcagcaaca gaagaatcaa caacagtgcc tgaatcttca
    14701 gcaacagaag aatcaacaac agtgcctgaa tcttcaacaa cagaagaatc aacaacacct
    14761 gcgcctacaa caccatcaac agatcaaagt gttgatacag gaaacggcac aggaagtagt
    14821 actccggctc caacgccaac accaacacct gaacaaccaa aacctgtaac acctgctcca
    14881 gctccttctg gaagtgtgaa tggtgcagca atcgtagcag aagcttacaa atatattggt
    14941 actccatatg tttggggcgg taaagatcca agtggctttg actgctcagg gttcacacgc
    15001 tatgtttaca tgcaagtaac tggtcgtgac atcggtggtt ggacagttcc tcaagaatca
    15061 gctggaacaa aaatttcagt ttctcaagca aaagctggag acttgttatt ctgggggtca
    15121 caaggcggaa cttaccacgt agcgattgct ttaggcggag gacaatatat ccatgctcct
    15181 caaccaggcg aaagcgtaaa agttggttca gttcaatggt ttgcccctga ctttgctgtc
    15241 agcatgtaag aatga
//
LOCUS       DAYYGO010000066        15138 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000066 DAYYGO010000000
VERSION     DAYYGO010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15138
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.068"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..2377
                     /locus_tag="ACHA5T_001769"
     CDS             5..2377
                     /locus_tag="ACHA5T_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFM8887339.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEEYVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESAFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     RPTDSMKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTLETPENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2438..3898
                     /locus_tag="ACHA5T_001770"
     CDS             2438..3898
                     /locus_tag="ACHA5T_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFM8887340.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQIKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3909..6113
                     /gene="topB"
                     /locus_tag="ACHA5T_001771"
     CDS             3909..6113
                     /gene="topB"
                     /locus_tag="ACHA5T_001771"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM8887341.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLRAEEISTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYES
                     LTPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGVLLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSIDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            6129..6278
                     /locus_tag="ACHA5T_001772"
     CDS             6129..6278
                     /locus_tag="ACHA5T_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887342.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKLRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6353..6946
                     /locus_tag="ACHA5T_001773"
     CDS             6353..6946
                     /locus_tag="ACHA5T_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFM8887343.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            6959..7858
                     /locus_tag="ACHA5T_001774"
     CDS             6959..7858
                     /locus_tag="ACHA5T_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFM8887344.1"
                     /translation="MTPTQFAAKTRNLAKRTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTAQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIIGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            7861..8358
                     /gene="ssb"
                     /locus_tag="ACHA5T_001775"
     CDS             7861..8358
                     /gene="ssb"
                     /locus_tag="ACHA5T_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFM8887345.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMVTLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNALQNPFGNPFGGQSIDISD
                     DDLPF"
     gene            8724..8984
                     /locus_tag="ACHA5T_001776"
     CDS             8724..8984
                     /locus_tag="ACHA5T_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887346.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            9429..9635
                     /locus_tag="ACHA5T_001777"
     CDS             9429..9635
                     /locus_tag="ACHA5T_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFM8887347.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGQRAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9656..9886
                     /locus_tag="ACHA5T_001778"
     CDS             9656..9886
                     /locus_tag="ACHA5T_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887348.1"
                     /translation="MTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLVEDAPTML
                     FEDLVKIAGYELHYQENMETYENEYWLTKLEN"
     gene            9902..10339
                     /locus_tag="ACHA5T_001779"
     CDS             9902..10339
                     /locus_tag="ACHA5T_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887349.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            10332..11039
                     /locus_tag="ACHA5T_001780"
     CDS             10332..11039
                     /locus_tag="ACHA5T_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887350.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKHRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSCTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATIDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKRQ"
     gene            11203..11403
                     /locus_tag="ACHA5T_001781"
     CDS             11203..11403
                     /locus_tag="ACHA5T_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887351.1"
                     /translation="MNKIIPLLFVLCIGEILMRHGAAVHLNFVVFLGILVFCLIFWRF
                     VLALVSLLFKLIAAIFFIGLFR"
     gene            11419..11598
                     /locus_tag="ACHA5T_001782"
     CDS             11419..11598
                     /locus_tag="ACHA5T_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887352.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            11923..12186
                     /locus_tag="ACHA5T_001783"
     CDS             11923..12186
                     /locus_tag="ACHA5T_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HFM8887353.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            12180..12530
                     /locus_tag="ACHA5T_001784"
     CDS             12180..12530
                     /locus_tag="ACHA5T_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8887354.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(12554..13168)
                     /locus_tag="ACHA5T_001785"
     CDS             complement(12554..13168)
                     /locus_tag="ACHA5T_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8887355.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            13482..13904
                     /locus_tag="ACHA5T_001786"
     CDS             13482..13904
                     /locus_tag="ACHA5T_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887356.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            13914..14117
                     /locus_tag="ACHA5T_001787"
     CDS             13914..14117
                     /locus_tag="ACHA5T_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887357.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            14328..14912
                     /locus_tag="ACHA5T_001788"
     CDS             14328..14912
                     /locus_tag="ACHA5T_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8887358.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT         5570 a         2451 c         3061 g         4056 t
ORIGIN      
        1 gaacatggca gaagaaaaag aaccactaga caatagtcgt ttgggaaaat ccaaaagaaa
       61 actagtacga ttgcaaaatg aattaaacga acaaattgaa aagatgtttg aacaccaaaa
      121 aatgacgaac ggtcaaccaa tgaacgacaa gaggaatggc cgttcatggt ttaaccagca
      181 agaacgaatc gaaaataaag tccaaagtct gagagaagag atcaagcaac aagaagaata
      241 cgtggaaaag cttgaaagac aagaagagct aaaggaaatg ggctataaca agtacggcgg
      301 actggatatg acgatcgata atattccaag aatcaaagaa gaaatcgaac gctttgaaaa
      361 aggtgaatcg gctttttcag ccgccacgat ccgaaaatac caacggaagt tggaaacatt
      421 agaacagctg aaagaaaaat cagaaaaagg aaaagaaaat atcctccctg aagtacaagc
      481 gatcattgac agcggacgag tgacgcaatg gaaaaaaaat ccaaccattt attttttgaa
      541 aggctatcga aaagttgctt tagaactgaa tgaaaacggg ggatttgagg aatcaaaaaa
      601 ataccaagcc acgtcggaag aggaaaaagc agctgttcgt gaattacttg aagaggctag
      661 accaactgat tctatgaagg agaaagaaaa cgatcatttc tatcgagtag aattcaacga
      721 aaatgatcca gaaaaaggat tgaaaagtta tggtggggag atcgtaacat tcgaattgat
      781 cgatcaactc aaaacattgg ataaccaata cgccgcagaa gcaggttatt ataaattcta
      841 ttttgaggaa gtgaagaacg gagaagtagt cgatcagatc cgaatggatc taggggatgg
      901 cgtcaacagt aatcaaccaa tctatttaga gttagcaaaa ttgtgcgaac aagtacctga
      961 gaaggaacaa aaacaatcgg tcaaaaatga acctgtttcg aaagaacaaa tgcagtcgaa
     1021 agaaacaaaa acaaaacaaa agggctatca aaaacgaacg gcagaagaga ttaaacaaga
     1081 agttaaatcc ctttcgcaac aggcactgga tgcggtgaaa aagcataccc attctcctga
     1141 agacgtcaaa gaacttttga actttatgtc tcgttttcca gaacgatcct ttagaaatca
     1201 attattgatt gaggaacaat ttccaggtgc aacggcttgt ttaggtcgtg ccgcgttgaa
     1261 aaaagaagga atctatatca aaaaagggga acaagggaac aagatctttg tcagaaaaac
     1321 ggtcaaaggg ttttatgaga aaggacgtgg ctttgttcga gaaacagaag ctacaccaga
     1381 agataaaaaa cgaatcgaaa gtggtcaaat cgaagtgatc aaaaaacctt actatacgat
     1441 tgaaaaagtg tttgatatca cacaaacaca gttaaaacct gaagactatc cgaaaatctt
     1501 tccgaaccgt gtctttgatt ttcaattgga taaaaaagga cagtcagaac ttcaagctgg
     1561 gattcaagcg gtagccaaca atattggaat acaaattcga gatatgacag aaagtgaagt
     1621 ctatcaaaga gaattaggac aagcacgtgg ggcgtatgtt cgtaacgaat ttactggcga
     1681 agaagaaatc gtcatgaata ctcgaaatac tcctacgcag catttagcta caagtattca
     1741 tgaattagcc catgcaagaa tgcacaaatt ttcagaatta gataccgcaa ctaaagaatt
     1801 gcaggcagaa atgacttctt atattgtgtg caaacatttt ggaatggaca cgtcggagaa
     1861 agctattcct tatattgcag catggacgaa gaatggtcag acgttggatg ataaggaagc
     1921 tgcggaacgt gggaaaatca tgaatgatgt cagccgtgtc gcaaatgaat tcattcaaac
     1981 catttctaat gaaatcaatc aatacagaga aagagaacca gagatccaac cagcagaagt
     2041 gaagcaggag cataaaaata ctttagaaac gccagagaat aagaatattg tgcccgttgg
     2101 ttcactttgg atcgacaaga aagacgggaa agtaatggaa gaagatacgg gtcgaacgtt
     2161 acatttgaag gatgcggcgt ttattgaaga tccgcagcat aatcaagtaa ttttaaaagg
     2221 aaaaagttat ggaaaagaga tccaaacggc gttaccttca gaagaatttg cgctaggaaa
     2281 tccattccga ttttcacttg agacagtgtc agaagtggca aaacgaaaaa atgaaatcta
     2341 ttatgaacaa caattgtcac aaaaagaacg tgtataaaac aagtaaaaaa gactatctga
     2401 aaaacggata gtcttttttc ttttgggagg ttttaaaatg accgagaaaa aaatgagtct
     2461 tattgatcga tgtaaacaga tcgatatagt ggattttgct cgaaacaatg gaatggctgt
     2521 agtcaataaa ggacgagatt atcgcttaga agaccatgat tcatttgttt ttgatcgaag
     2581 aaaacaacgt ttttattgga acagtcaaaa tattagtggc gacattattg aattagctaa
     2641 actttttttc atagacaaag agatccaaga ccctaagcaa caatttaaag cagccactga
     2701 ttttattcta aagaatgaag acaaaacaga acgagtggaa aatcttcatt ttgaaacaga
     2761 aaagtacaaa gatcaccccg ttgattatca accactcaca gaaaaaggaa ggaattatct
     2821 gaaggaagaa cgaaaactac cagagtggct gattgattat gcagaaaaag aagggttgat
     2881 tgccgaacta aagccaaaat atgaacggca aaatttctta gttcgagacg atcgtttaga
     2941 tcatgcggtc gcctttttat ggaaagatcc acagacaaaa gagacagtag gggcgagtta
     3001 tcaaggaacg ttcattgatt atgaacgttt tggtgagaga ggcacatata agcatataga
     3061 taaaaattcg acagcgaatc atggctttaa cctaaagatc ggtgacccta agcagataaa
     3121 attttttgag agtagtattg atttacttag ctatgctgca ctgaatcgtg atcagttaaa
     3181 cgatacgtgg ttagtctcta tggaagggtt aaaacaccat gtgatcagtc attattttgg
     3241 tgaggcagta tctgaattga gaaagaaaca agcctttcct caatccatcg agatttgtgt
     3301 ggataatgac cgtgcaggac atattttcta cgaaaaagaa caattaatgg gcgcagtcga
     3361 tccatttacg aatcagaaag tacgttgtga acgaggaatc gctaatgatt ggcaagtgcc
     3421 aaaagaatac aaaatcattt atgaagaagt cgcaaaagaa gaaaaggtca caccagaagc
     3481 tattatggcc atacacaaga cagaaaacaa tctgcaatta acgaaccaac tggtttccgc
     3541 tcacaaagta aatgcgtttt ttggccagca gctttctgtt aacgattcaa tagaagccat
     3601 taatttgaaa gatatttgta gagaagtcgc aaaagaatta aaagtttgtg aacgcgtgga
     3661 tggtacgtac gattttgatc gtttttatca agaaaaaggg gatattaatg cgcagattct
     3721 tttctcttat aaagcagaac aatactacaa aggctataaa aatcatgaac atgaatttgt
     3781 acctgaagta aaaaaggact ggaacgatca attgaagcat gagatccagc aacaagaaat
     3841 cagaaagcaa aaacgtgcca tgttgtttca acaaggcaga caacaagaaa gggagtgaaa
     3901 tcgtcatgat gaaaaccgtt attttagcag aaaagccgtc acaagcgaaa gcttatgccg
     3961 atagcttctc taaagccact cgaaaagacg ggtattttga aatccaagat cgattgtttc
     4021 ctggtgaaac tgtgatcacg tatggttttg gtcatttagt cgaattagat tcgcccgata
     4081 tgtacgatga aaattggaaa caatggtcat tagaacattt gcctattttc cctacacatt
     4141 atcactatca tgttcctaaa gataagaaaa agcaattcaa ggtggtgaaa cagcaacttc
     4201 aatcggccga caccattatt attgcgacag atagcgatcg tgaaggggaa ctgatcgctt
     4261 ggacgatcat tcaacaagta ggcgccgatc aaggaaaaac ctttaaaaga ctttggatca
     4321 actccttaga aaaagaagcc atttatcagg gattccagca gttacgagac gcaaaagaga
     4381 cctatccaaa atttgaagaa gcacaagcac gtcagatcgc cgattggtta attgggatga
     4441 acggtagccc actgtatagt ttactcttgc agcaaaaagg cataccagga agcttttcct
     4501 taggcagagt ccaaacccca acgctttaca tgatttatca attgcaggaa aagattagaa
     4561 atttcaagaa agaaccttat tttgaaggaa aatccgaagt aatcgctcaa aatggcgcat
     4621 ttgatgcgaa gcttgatccg aatgaaaccc aagcgacgca agaagcattt gaagattatt
     4681 taaaagaaaa gggggttcaa ttgggaaaac agccaggcat gattcgccaa gttgaaaccg
     4741 agaaaaaaag cgcggcaagt ccacgtcttt tttctctatc cagccttcag tctaaaatga
     4801 atcagctcat gaaagctagt gccaaagaca ctttggaagc catgcaaggc ttgtatgaag
     4861 ggaaatatct aagctatccg cgaacggata ccccttacat tacggaaggg gaatatgcgt
     4921 atctgttgga tcacttagac gaatacaagc attttttgag ggctgaagaa atttcgactc
     4981 ctattcatac accgaatagt aggtacgtga acaacaagaa agtccaggaa cattacgcaa
     5041 tcattcctac aaaaacagtt atgacagaag cgacgtatga aagtctgaca cctttacaac
     5101 aagcgattta cgagcaagtt ttaaaaacga cagtggcgat gtttgcggaa aaatatacct
     5161 atgaagaaac aacgatcttg acgcaagtcc agcagcttca gttaaaggca atcggaaaag
     5221 taccgataga tctaggttgg aagaagttat ttggcaaaga gagcgacgga aaagagaagg
     5281 aagaagagcc gctacttcct aaagtgacaa aaggagaaat ggtgacggta gatctacaag
     5341 tccttgaaaa agaaaccaag ccaccacaac cttataccga aggtacgttg atcactgcga
     5401 tgaaaacagc tgggaaaacc gtggatagtg aagaagcgca atccatattg aaagaagtgg
     5461 aaggaatcgg tacagaagcg acacgggcga atattattga aaccttgaaa caaaaggaat
     5521 atatcaaggt agagaaaaat aagcttgtgg tgacgaataa aggggttttg ctttgccaag
     5581 ctgtggaaaa agagccgctg cttacgagtg ccgaaatgac cgccaaatgg gaaagctact
     5641 tgttaaagat tggagaacga aaaggaacac aaaccacttt tctagccaat atccaaaagt
     5701 ttgtctctca cttattggaa gtcgtcccag gacaaatcca atccattgat tttggatcga
     5761 cgttacaaga agtgaaagca gccagtgaaa agcaagaagc cgcgcgtcat ttaggaattt
     5821 gtccaaaatg ccaggaacaa gaagtcttac tctatcaaaa ggtggcagct tgtacgtctg
     5881 aagcctgtga ttttaaatta tggacaacga ttgccaaaaa gaaactcaca gcgacacagc
     5941 tgaaggagat tattcaaaat agccgaacaa gccaaccagt gaaagggtta aaaggtcaga
     6001 agggttcgtt tgaagcaacg attgttttaa aagaagactt cactacaggc tttgaatttt
     6061 ctgaaaagaa aaaaacgaac ttcaagaaaa gaacacgacg cacgacaaaa taatggaggg
     6121 ttaatcttat gcgtaataca acacacaaac aacaagaaat cgaaaatcaa aaagccaaaa
     6181 ttcgttcaga acaagagaaa ttacgtgcca gtaaaagaag acttcagcaa ttaaagaacc
     6241 agcaacggat tcaaaaagaa atcgaattag atcgatagac attaaccttg ttacaaatac
     6301 actatcatga tatatttaat gtagtgtatt tgtaacaaaa ggggggaagt aaatgcaaga
     6361 gaaactgaaa gaattgttgg ctattcgcga tggaaattta tctatgaagg aagccagaaa
     6421 attaggaatc gcagcaacca ctgtacaaag attggtgaat caaggaaaat tgatcaaagt
     6481 tgatcgtggt tattatgtag aggatggtca tggaatagat gatttatttt ggcttcagca
     6541 acgtttttcc aggggaatct tctctcatga aacgacttta gatatttacc agctttctac
     6601 gaatatgccg aaattcattc acttaacttt tccccatgga tataacgttg atcgttcaat
     6661 tacaaaagag cagcagttac aatttcattt cgtgaagaaa gaagtttatg aactaggaaa
     6721 agtagaggga acttcctttc aaggaaatcc tattactatg tatgataaag agcgtacact
     6781 ttgtgatatt tggaatccga gatatgaaat tgaatatgaa atgaaattag aagcattaaa
     6841 agagtacatg gaagacccat tgagagatcc aagtaaatta agagattaca tgacaaaact
     6901 acatgtagaa aaagaaatga aataccacat gcaaagctta tattgatgga ggaaaaagat
     6961 gacaccgaca caatttgcag caaaaacaag aaatttagca aagaggacag gattaaatgc
     7021 acagttagta caacggcatt ttatgatgga tcgtttaatt gaacaaattt ctgaatctaa
     7081 gtataaaaat gatttcattt tgaaaggtgg ctttttatta ggatcaaaat atggaatcga
     7141 tagaaggacg acaattgata tcgacacgac atttagaaat agcaaattaa ctgaagaaaa
     7201 attgaagagt atttttaatg atttaactag gaaacccacg aaagaaggta ttgttttctc
     7261 tattcaagga atgaaggaaa caagagaagc cgatttttat cctggatttc aaattaaatt
     7321 ggtggctaat ttagagaaag tacgtgtgcc atttaaaatg gacgtgacaa caggagattc
     7381 tatttatcct gaagtagata cacactatca ccaattaatg tttgaagata aaaaggtgga
     7441 gattccagct tatccaacag cacaaattat tgcagaaaag ctaagtgcta cgttcagctt
     7501 tggcacagat aattcgagag caaaagatta ctacgactta tttaccattc cgaaactaga
     7561 aaatatcgat acgaaagagt tatacaaaag tgttcgaaat acatttacaa aacgtgggga
     7621 tacaaaaccg ttgtcctttt attatgaaag agaaatggat aagatcatag gaaatgacta
     7681 tttggaaaag cagtgggaaa aatatcgctc agttaacacg tttgcgaatt ccatttcatt
     7741 tacagataca gtcaacgaaa ttgatcacct aatgaaaagt gtgatcaaaa ttgaaaatca
     7801 agaaagaaaa cggcagttta aaagatcaca acaaatgaat caagaaatgg agagataaag
     7861 atgatcaata atgtaacttt agtaggacgc ttaaccaaag atccagattt acgttatacc
     7921 gcgagtggta ctgcagtagc gacttttacc ctagctgtca accgaaattt cacgaatcaa
     7981 aacggcaatc gagaagctga ttttattaac tgtgtgattt ggcgcaaacc agcagaaaca
     8041 atggtgacat tagctaaaaa aggtagcttg attggtgtag ttgggcggat tcaaacgcgg
     8101 acttatgaca accagcaagg acaacgtgtg tatgtgacag aagttgtggc cgataatttc
     8161 caactattag aaagcaaagc agcgacagaa agccgtgcgc acgctgatca atcgtccacc
     8221 agtccaagca ccaccacttt tgaacaacgg gatacagcga cgcctaataa caatggattg
     8281 aacgctttac agaatccatt tgggaatcca tttggtggac aatcaatcga tattagcgat
     8341 gatgatctac cgttctaagt agcattttct attggttgca tttgctacgg tttatgttat
     8401 aatttagtca acaaaaagaa ttttcaatct ataaccaaaa accactagct ggcgggctag
     8461 tggttttctt ttacgtttct attataagaa ttaacgggta ggcttactgt ctttcttggc
     8521 aaatttacaa tcaagccatt tgcagatata gtgcgcggct acttctgcca tgattgcaaa
     8581 aacaaaatca aaaagaattt tcaaacctat aacctcctcc ccttgccaga ttaggccggg
     8641 gtagcagaca gcaagactat tatagcataa ttgaaatatc caaatgattg gatttttgct
     8701 ataattttag cgaggtgatt gtgatgaaca aatatgttgg tttgcttgat aaaatccgag
     8761 taataaaaac gcaaccgtta ttggttcgct ttactttaca aacgattcat gaatcgatta
     8821 attgtgtagt tgcagatatt gaaattatcg ataaattgct aattatggat gatggaaaat
     8881 acaatattgc cgtcacaggt cacttcaata aacgaaatca gctagttatt gctagcatgt
     8941 atgttagaaa tcccgatcat tttacgcgat cgatgggcat ataaaaaaag aagagttgca
     9001 atcctaatgt aggactgcag ctctttttgt tttacataga aacaagaaaa atgaaaaggc
     9061 ctttttagga aggcgcgagc gccttctcaa ttgaaaaacc tctctcactc caaccgccac
     9121 cgccgttcga gaagaaagga agtgaacgtt gcacgttcac taagataaag ggtcttgcaa
     9181 agcaagacaa aatagaaaaa acttcctctc accaaaaggt tcgttcctgt tcgttatctc
     9241 tttattgatt tgccagaccg gcaacaagtt gccttttatc accaaaaagc gacaaggaca
     9301 agggtaaatg gcacgctgcg cgcccttgtc cttgtcgctt ttcgttgatt cattctggcc
     9361 aacaaatcaa taaagaaaga actaacgaaa ggaagtaaaa cctttcctaa gaggaggcag
     9421 aatcaatcat gcgaaagaaa tttgattatt ggggtgttcc cttcagcgaa ttattcccaa
     9481 actatcatgc accacatact gtcgaatgcg attgtggaca aagagcgaaa tgtatcaaaa
     9541 gctatcgttt gtatcaatgt ccaacatgcg gaaaaaaata caccttgtct tatggcgatt
     9601 atattttgat tgatgagaaa ggaaagaaac gctgatgaaa acataacata aattattgac
     9661 tgtaaaagaa gtcgttcaaa ttttggtgga caaagaagtc atttctgaaa aagtagcaga
     9721 agctttactt attccgcggt actggacgat agaaacccct ttagtagagg atgcaccgac
     9781 tatgctattt gaagacctag tgaagattgc aggatatgaa ctgcattatc aagaaaacat
     9841 ggaaacttat gaaaacgaat attggttaac caaattagag aactaaaaag gagaaaaaaa
     9901 tatggctcaa aattactatg acgaatttgt caaattacct ttggataaaa tggcacaaaa
     9961 aatggaggat atgacattcc tataccatga gacacgtgtt ccaaaaaaac attataagga
    10021 aagattgtcg gtagctgtgg aagaaatgat tgaatcaggt gtcgaaatga atctcattgc
    10081 gacgtactat cgaacactgg aagaactgaa aaaacaaaat gcaaaatggt ttttccaagc
    10141 cttactatgc ttggaagtag gtgtcaaacc aagcactatt aaaccttctg aatatcaagc
    10201 tttagaacta acgtatggga aatttgtcga aacgaaaaaa gccaagactg tttcaagtga
    10261 atggttagat tattttgaaa atatcaataa gtatggcgcc tattacacca tgaaaaagga
    10321 ggacaacgaa aatgaataag gtgatttatc aactaacaac ggaagatgtg tcacactttt
    10381 taacaaacaa agaacatttg aatgaagtaa tcagacggat tcaaaaagac tttagttgta
    10441 tggaagaaat gaagtacttt attcaaagag tcgaagaaca attgtatttg gaaagtgaaa
    10501 cgagtcaaac gaaggtcaca gtcagcaata attatttaga cgtgtataaa catcgtcctg
    10561 aaacggtaaa aaaaggaacg gagcttctac gactagaaac agaaaaatac atcatgaatg
    10621 tttacctaga aaggagtgaa ctggtcgaaa actttgtcag ctgcacgatt tgcaggaaac
    10681 actattactc agaatcagaa tattattcaa tttttgagag catggaatta atggaagaag
    10741 aaatggaaga gctaaacagc aaaaatgcga cgatagacga ttattatctt gtatttacta
    10801 gatttttgac gtgtggaacg gaatctatcg tgttaatggg aatggattat gaactcgctg
    10861 tacccgaagc atttttgagg gaatatgtag aagaagtaga taacccaggc gacgactggt
    10921 acacgacttt aactgatttt ttagatacct acacttacga cgatacagaa gaaatccaaa
    10981 atgaacttga ccgtaaacga atgccatatc aacgatttga aaggaaaaaa aggcaataat
    11041 catttatacc gaaaagtgag aatgtgtgaa ataagaacaa aaaccttgaa attaagggaa
    11101 tggttcttat ttcgcaccaa tcgctccaac cgcccccgcc gcttaggtgg tgcgaaataa
    11161 gcgggcaaag cccgataacc taagagaaag aaggagatcc atatgaataa aatcattcct
    11221 ctgttgttcg tactgtgtat tggcgagata cttatgcgtc atggtgcagc tgtacattta
    11281 aacttcgtag tattcttagg aatactagta ttttgtttga ttttttggcg ctttgtgtta
    11341 gcccttgtga gtctactatt caagttgatt gcagccatat ttttcatagg gttatttcga
    11401 tgaaaggaga gagtactaat gccaattgca ataggaaata aacggttacc cgtgacatta
    11461 gatgaaaaga gacaaaaaga attgcagcaa ctaaagcaga agtacggaaa aagtgaatct
    11521 aagattatgt gtattgcgtt agatctattg atcgcgcaag aaaaagcagg atttgatata
    11581 ccagcactaa aaaagtgacg tcacttttca tttgaagaaa caaaagtgat agcacttaat
    11641 tgttcctact atttagaata ttttctatgt cctttttatt ccattttgta tcaataacta
    11701 tgaaatcttt aaagtatcca gatcaatttt tttcttacct gattctaaag attttgtttt
    11761 cttaaacata ttatgatttc ctttcgacta ttttcatata aatgttaaca tagtttatgg
    11821 attcatggaa tgttaatcta atttaataga aaaggataat taagtatata cgttttttct
    11881 tatgtatgtt atactatgta tatacagaaa gaggtgtcta ctatggaaat gttacctata
    11941 aaaaaatggg gaaatagtaa tggccttaga ttgccaaagt atattatgga atatcttgga
    12001 attcatacag aagacaaagt gaagataata caagaagaag taaacggtca gaagcggcta
    12061 attatcgaag cggctaatat agaaaatgaa ttaacaattg aacagttatt tgaaaattac
    12121 actgatgaaa gaaatcatgt gacgattcaa aatttaggtg aagcagtagg taatgaaaaa
    12181 tggtgagaaa acctagacaa ggcgatattc tattattgaa tacagcacct cgatctggac
    12241 acgaacagac aggtaaaaga ccttatattg ttttaagtca tgatattatt gcggattata
    12301 gtaacgttgt tacagttgct ccaatttcta gtacaactag aaactatccg ctatatgtaa
    12361 atataaactc agaatatgaa atgaagacaa caggaaaagt attattagat caactaacga
    12421 caattgatta tgaagccaga gagtgtattt ttttggaaaa agcgcatgat agtttagttg
    12481 aggagttatt gatgaaagtt agaacagttt ttcaaaaagt aagcaaataa aatagaaaca
    12541 gcagcatgat caactattcg tgattgacca tgctgtcttt ttttattcta tatacggttt
    12601 gtctcgtaac attgtagtct tttgcaatct tagcgatcgc aacacctttt tttagatcct
    12661 caacgatgtt tttataaaca agacgacgtt ggggatcttt tgcattggca ctgtatagct
    12721 taggacgtcc tttataaaca ccgttcgctt ttgcaatctt aatgccttgt gcctgacgtc
    12781 gtttagattc ggtacgttct tgttcagcga tcatggccaa aatttgaatg atcaaatctt
    12841 taatgaattt atccaggaga ggattgccaa ttgcttcggc catgatcggt agacttgtga
    12901 tgatcagctg cacatttttc ttcttcaagt aattgactga agcgatgatc tcgtcgtaat
    12961 ttcttccaag tcgatcaata gcttccacaa taaaaatatc ttgatctcgg acgaaatcca
    13021 gtgcttcttg aaaaacaggt cgatgagtaa tcgttgcgcc agattgtttc tcaatgaaaa
    13081 ttctctctgc accgaatttt ttcatttctt caatctgccg ttgttcattt tgatcgatgg
    13141 atgagacacg tatgtatgca actctcaaaa tttttaccta ctttctgcaa ctatttaaaa
    13201 aacaccctaa tcatacacga gaaaaccaga tttttcaatg tcgcggtcgt gtctagttag
    13261 ggtatacctt atatagacaa aaactaatga tggaggaaat agtatgtgtc acttttcttt
    13321 ttaaatggca atattttttg taaataaatg tcaatcaaga aaatttaaac gattatgaca
    13381 ttaaaaatat attttatgaa ttttctagta tataacttga tttatctgtt aagatttatt
    13441 aagaaaacgt tttcttattt ttgatttcga ggtgatagac aatgaaaaat aagtttattt
    13501 tatctttagg tattttaact attggaatta gtgtaactgg aactacaatt cctacatttg
    13561 ctgatagtac aaatgaacca actactgtag tagataataa ccaagaatta actcctttag
    13621 ataggaacgg aatagccatt ggtaacccaa ctaacgactt aactgttact gaaaattatt
    13681 taatagtttc tccattactt gctcgagcag cttctaaaac gatacgagtg acaggaacga
    13741 tgatttttga taaagttggt tggccttcta gacctcctca atcacggaat tggaaagaaa
    13801 aaagaaatgg ttattggtac tatggaaaat tgtatttaca aagttataaa tcagctggaa
    13861 acaattggat tgcgacttat tcaggaacct taactagaaa gtagggattt tttatgccag
    13921 aatttgtgaa cacaccaaat ttttatcaag atagcgatat tctaagtaaa attaatttac
    13981 cgaaagaaga atcagcttta caagttcagc gaactttaca ggagacacaa aaaattcaaa
    14041 aatcaatttc taatctagaa gattctgaag aaaaaagaaa aaaaagagac caaaaaacat
    14101 atcaaaaaga aaaataatga ttttaaaaaa agagtgtcta gtcgacattc tttttttcta
    14161 attgaaagtg ttcataaaat cacccatttc tggacacttt ctttttttgc tttatttttc
    14221 aaaaaaacaa ttcatttcat gttcataaac ccataacaat atctatgttc agttaaacta
    14281 tatggataaa gttatgttac aataaaatta atgaaggagt gatttttttg aaaataggat
    14341 acgcccgggt atctactggt ttacaaaatt tggatttaca agaagacagc ctaaataaat
    14401 ttggttgtga aaaaattttt actgatcata tgagtggggc aaaaagtaat cgaccaggat
    14461 tagaaatggc cattgatttt gttcgctctg gcgatacact tgtcgtttgg cgcttagatc
    14521 gtttaggtag aaatatggaa gatttgattt caatcgtcaa tcgactgaat gagcgtggtg
    14581 tgagttttca tagtattcaa gaaaatatta cgatggacaa atcaagttct acaggacagc
    14641 tcatgtttca tttgtttgcg gcatttgcag aatttgagcg taatttgatc ctggaacgat
    14701 ccgcagcagg acgagcagct gctcgtgcaa gaggacggtt tggtggtcgt cctgaaaaat
    14761 tgtctgaaca agatttggaa ttactgaaga ctttagtgga tagcggtaca cctattaaga
    14821 ctattgcgga acggtggaac gtctcaagaa ccaccattta tcgttatttg gcaaaaatgg
    14881 aggagaaaaa tggaaagaca aattctcatt agtttttgtc atttaatgct gaaaattttt
    14941 gccatttatc tagaaaagtg acaatagtat gagaattttt tgtaagtgtc tactgataac
    15001 ttattctcaa atgttgtaag gattttattc atttgttaat attaaagaac ggaactccaa
    15061 aaaagtccgt actctaattt gcagtctctc actcttttag agtgagagtt tttattagat
    15121 tttcaaatta attagtaa
//
LOCUS       DAYYGO010000067        15003 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000067 DAYYGO010000000
VERSION     DAYYGO010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.069"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(617..958)
                     /locus_tag="ACHA5T_001789"
     CDS             complement(617..958)
                     /locus_tag="ACHA5T_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8887359.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(958..1188)
                     /locus_tag="ACHA5T_001790"
     CDS             complement(958..1188)
                     /locus_tag="ACHA5T_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8887360.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            1475..1813
                     /locus_tag="ACHA5T_001791"
     CDS             1475..1813
                     /locus_tag="ACHA5T_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887361.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(2018..2580)
                     /locus_tag="ACHA5T_001792"
                     /pseudo
     CDS             complement(2018..2580)
                     /locus_tag="ACHA5T_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(3312..3893)
                     /locus_tag="ACHA5T_001793"
     CDS             complement(3312..3893)
                     /locus_tag="ACHA5T_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8887362.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(4076..4702)
                     /locus_tag="ACHA5T_001794"
     CDS             complement(4076..4702)
                     /locus_tag="ACHA5T_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFM8887363.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(4724..6055)
                     /locus_tag="ACHA5T_001795"
     CDS             complement(4724..6055)
                     /locus_tag="ACHA5T_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFM8887364.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(6210..6611)
                     /locus_tag="ACHA5T_001796"
     CDS             complement(6210..6611)
                     /locus_tag="ACHA5T_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFM8887365.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(6697..6903)
                     /locus_tag="ACHA5T_001797"
     CDS             complement(6697..6903)
                     /locus_tag="ACHA5T_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887366.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(6998..8500)
                     /locus_tag="ACHA5T_001798"
     CDS             complement(6998..8500)
                     /locus_tag="ACHA5T_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFM8887367.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(8518..8973)
                     /gene="argR"
                     /locus_tag="ACHA5T_001799"
     CDS             complement(8518..8973)
                     /gene="argR"
                     /locus_tag="ACHA5T_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM8887368.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(9071..9688)
                     /locus_tag="ACHA5T_001800"
     CDS             complement(9071..9688)
                     /locus_tag="ACHA5T_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM8887369.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            9909..11282
                     /locus_tag="ACHA5T_001801"
     CDS             9909..11282
                     /locus_tag="ACHA5T_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8887370.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            11357..12469
                     /locus_tag="ACHA5T_001802"
     CDS             11357..12469
                     /locus_tag="ACHA5T_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8887371.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(12541..14178)
                     /locus_tag="ACHA5T_001803"
     CDS             complement(12541..14178)
                     /locus_tag="ACHA5T_001803"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFM8887372.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(14346..14954)
                     /locus_tag="ACHA5T_001804"
     CDS             complement(14346..14954)
                     /locus_tag="ACHA5T_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8887373.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         4089 a         3107 c         2638 g         5169 t
ORIGIN      
        1 cataattgtc gctttgagat tctactttgg ttcactatct gacccaattg cacattttcg
       61 atcattacaa gtattggtgt cgttagaaca actaaataaa aactagcggt agatttttca
      121 gaatctatgc taatttctgt atggatgttc cagtgatggc aaatttctct tttcattgtt
      181 tttcttgtcg catgatatgc ctttttgtgt tattcagtta aactatagaa attctttcct
      241 agacagatct tgatcccaaa ttttacgttt ttgagcatcg atatagttta aaatgacatt
      301 actcatatct aaagagttta gattttttag gactaaaact gtcagttgtt ctttttcttc
      361 tttctgttaa agtattgtcg cgacttttcg tatacgttaa gatatgtcgt gaaaaaacaa
      421 gttttgtgac acaaaaaaac gaatgacttg aggaaatcac ctcaatcatt cgtttaaaat
      481 gctatatatt ctttagttta agatacggaa attaatgcca tcgaccacac tagtagaatg
      541 aacgacattt acttggcctt tttttaattt cgttatgttt agcatataaa aatattatag
      601 agtttactta caatgcttaa aacatatact gtataatctg atcaatttta gccatgtctt
      661 gcaatggtaa gttttctatt tttttgagtt ttcgttcctt aaaatcaaga gattttaatt
      721 gtgtaataat tacttgtcct tttatatcta gctcttcagg taatgagtaa cgtgtcggta
      781 aattctttat agtggaagta attggacata tgaccgaaaa cattgtttta cggttaaatt
      841 cataacgaga gacgactagt cctggtcgtc ttttttgaat ttcttttcct acggagggat
      901 caaaatcaat ccatacaata tcaccttttt taggaatata aattctttct tctgacacta
      961 taaaatttct ctcccttcag gagttaaatc ttcccattcg tctttctcat aatactcggc
     1021 ctcttcccca ccactaaatg gatcttcaat ttttggaact aacgtaattg ttccatcatc
     1081 agaatacaca acgatatatt cttggttttc tgaaggtttc tttccgttat cagaaggtaa
     1141 agttaccact acagaacttc cttgtaatct agattttgta acaagcatta tcatcactcc
     1201 cttaataaaa gtatactata taacactaag taacgcaaaa ttcccctttg taaactatct
     1261 agaaaattgt tttgtttaaa gtatatctta ttggatataa atagaaataa attgtagcca
     1321 gttcattttt tctttaacta aaaaaataat ccaagaaaca ttgaacctat ggatccaaaa
     1381 gctacccaaa tactatagct aaccccccgc taccttcaat tttgcagata aaacgctttg
     1441 ggagaaaatt tcaaagacaa caggccatcc tatcatgccc gattataaac aaaaagctgg
     1501 ctattccttt tgctctgaca aatttcaatt gggaaagttc acgaaactag caggtgaaga
     1561 agtttcaact gttcggatca aagaatgccc gcttcaaata gaaacattgg ttcaacgaat
     1621 ttcaactaga caagactttg ctattgtaga acgtgagatc gtagctattt ttgtagaaga
     1681 agggatttta catgatccaa ctcatatcaa tgtggataaa tggcaacctc ttatctataa
     1741 attcagagaa tatacaactg ccaaccagtc gttaactttg aattttagat tccaagaatt
     1801 caaagagtca taatgataaa catcgttagg tataaataaa aaagactcgc agcacgagta
     1861 aaaaacaaat agaagctata cattagaaag gtcgactgtg accctataga tgcagtatat
     1921 gcagcttatg agcatttctt ctgttctctt tgtttttggg atctgtgctg aagtcttttt
     1981 cgttttttgc cgctctttct ttgaaaaatc gattatgtta taccttctcc attactaatt
     2041 caggcatctg ctgagtaatg atttttcgcg caaaagaaat atctgttacc caaggttcga
     2101 tttgttcttc agtcaagaca agcggcattc ggtcatggat tggtgcgact gtgcggttag
     2161 gttcagtagt cattatgatt gattctgcca ttttttcatc ctgatggcta cgataaaaac
     2221 cgccaatata cactattttt tccttatttg aaaacataaa tttgtctttt tcttcattcc
     2281 actcattggg aaaacacaac gattttgcaa aaatggttga tgaaaggtct ttttttcttc
     2341 tactgtctct gcacgggcat taatcatcaa ttgtcctttt ttaaatccgg taaatcccca
     2401 cttcatgatt tccggagtga ggatgttttc ttcctctttt cttaacgtca caacaagatt
     2461 tgacggaaaa acttctccag ctgctaattc tgctgcctgc ttcggttgtt tcgtctgggc
     2521 ttctcgataa taattctgaa ggagctctga ctctagatca aaaaaataac gaccacacat
     2581 cctaatccct cccaatcttt ttttctatca tacactgcaa caagaagttc gcaccaacaa
     2641 aacgctttca atttttgtag aactaaaaaa gttgtgacaa gtgacaaact ctccagtgaa
     2701 tggtattctc ctgccaactc ctcggtcaca agtcacaata ttcaacaaac atgagaagct
     2761 gctttttgaa attgttcctt gtgactcgga gtgaaacggc aggcttacga cctctgggta
     2821 aacggtgttc aaaagccagt ttctcggtat taagccaaat ttatgcaaaa atgtagaaag
     2881 ctattttcac aaaatttttc ttaatactcg aaactaagcg actttttcac aacctcttcc
     2941 ttaaacggtg ttcaaaagct tgcatctgcg gtaataagcc caaccaaacg aaaaatatga
     3001 gcagctattt ttatttggct gttcttatta cttgatgcag ggcagctttt tcacaacctc
     3061 tccactaaac ggtgttcaat cagctaacct tcggtattag ctcaaaaaac ggcaaaatat
     3121 gaggaactat tttgccgttt gtttctctaa tactcaggtc aaagcgctga tttcacaacc
     3181 tctaatacac ggtgttccag aagtagcttc ttcgaaaaat aagccgaaat ttcacaaaaa
     3241 tttgagaagc aattttcgta aattccttct tatttcttga agctgaccac ttctgtcaca
     3301 acctctaatt attaaagaac cttttgcgaa gttaacaatc tttctttgat caattgaaaa
     3361 ttctgcctaa gtagtgatag atcctgtgtg actttcaaca ataagatact tccctggatt
     3421 tcagcaatca acgctttggc ttgacggtct gcttcttcat cggacagttt ttcttcagaa
     3481 agtgccagga tattttgttt cgtatacttg acccattgat tgactaattc tagtagcaac
     3541 agacggtatt cttcatccac tgtactcaac tcaataatca aattacctac aggacagcca
     3601 tgataagtga tctgtgtctc atgatcagcg aaaatccagt ccaacatttc ttctaatgct
     3661 tcttctggct gttttccagc atttaagatg tcttcgaaca attctgtctg ccatacgtcc
     3721 atatgcgcat ggatcaccgc tagacaaact tctttttttg aagaaaagta ataataaaat
     3781 tgaccttttc ctgtatcaga agctgctaga atatctcgga ttgacgtttc atgatagcct
     3841 tttttataaa taagatcttt ggtcgcttcc aagatctttt cctttgtttt caatgatatt
     3901 ctcctttttc atttctcata gtcctatatt atacagtaaa ttcatcattt tttcttctta
     3961 atcgtttaaa aaagactgtg ccaaattatc tgacacagcc ctcactataa aactgttctc
     4021 atatagctag atgttcattg attcaagaac tgcgaggaca aaagcaattc ctatcttatg
     4081 atagttgctc taatacttcc tctgttgtca tcacttgatc tgcaataaag ccaaagttcg
     4141 tcaacgctgc atccattgct tcttggccag gagcaccaat tgcatctgtc gctaccacaa
     4201 cattgaatcc actctctacc aattcacgca tatgggaatc gctacacaag tttccgttca
     4261 ttccaccaag aattactgtg tcgattccat tttttctcaa ttggagcgcc aaatcattgc
     4321 tttctggtcc aaagatttta tgaggagagc tgatgatcgt attttcatcc aaataaggtt
     4381 tcaattcttc gataaaatcg gctcctgaac cttcttccac tgattcatat tgactttttc
     4441 tagcaaacat ctgctgctca agcatcatat gttctcctgc accgccaaat tgccatttat
     4501 ggtcatgtgg gtaaaaataa tgaggagaaa taaagagttt gtatcctttt tcttttgctt
     4561 gttttaccat ttcaatcata ttttcgatcg tattgtattt ttctaagata tcttttgtca
     4621 aaccaaagcc accgcctgct ggtttcaaga actcgacttg cgggtcagtg agaactactg
     4681 ctgtattttt attattgaac atggtggatt ctccattcgt tatttatttt aatacatcat
     4741 ttagagcttc actcattgtc ggatgagaat agatttgatc tctcaataac gtataaggaa
     4801 gttttccatt catagctagc gaaatcaagt tgatgacttc atgagaatct tccgcataga
     4861 tacttgcccc tagaatcaag tcagtttcag ggtcgactaa aactttgaat acacctttag
     4921 gatcttctaa tacttgtgct ttagggacac cgcttgtcat atacttgaat aatttgtaat
     4981 tgacgccttg tgctttggct tgtttttctg ttaaacctac attggataaa gtcggagtga
     5041 taaatacact gtaaggcaca gctttccggt tattcgttgt tcgtttattc tcgcccttca
     5101 actgatccat aatgattcga taatcatcta gagagatata agtgaactgc aatccgcctt
     5161 tgacgtctcc aatcgcccaa atgttgtctg catctgtacg aaggtattca tccacagcga
     5221 ttgctccgcg atctgttagt ttaacatctg tattttctaa tccaagtgat gctgtacttg
     5281 gttttcttcc agttgcagcc aaaacttttg acgcttggat tgttacttct tggccatctt
     5341 ctgtaaaggt gacggcagct gttgattctt ggtctgctac ttgatctaca gatacaccta
     5401 gatggaattc gattcccgca tttgtcatgt cttctaaaat catttgcgcg atatcttcat
     5461 cttcacgtgg aagaaattca gaatgggcat caagaacgac aactttagat ccgtattcat
     5521 tgaacattga tgcaaattcc aagccaatat atccagcccc gatgatcact aatttttccg
     5581 gtaattgttc ctgatccatc gcttgcgtag aatcaatcaa gtagcgactt gtattcaatc
     5641 ctttgattgg taaaatgatt ggttcagaac cagtattgat gaaaatccgt tcgccttcaa
     5701 caatcacttt ttcaccatta ttcgtcactt cgatttgatg gttagaaaga aaacgtgctg
     5761 taccgtccat cactgtccca gttgcctcat cgctgatcat atgataattt ttggcgcgaa
     5821 gcatgcccgt caatttttct ttccgagcaa cagcctctgt aaagtcgatt cctctttctc
     5881 cattgaaaat caatgattta gaaggaatac agccgatgtt gatacatgtt ccgccataca
     5941 tacgtgttga cttttcaacg actaaaaccg attcaccttt tgaagctaac gccttcgcta
     6001 aagtcttacc gccctttcca aaaccgatca caatattttg atatttttcc atcatttttc
     6061 cctcgcttct tttttaccta ccgattggta caaaaatatg ataaccgata attgtctatt
     6121 ctccaaatca tctgctttat ttgtcaaaaa aaaccagaca aaaagcggtc ctttcacgtt
     6181 tttagtctgg ttcgatttcc tttattcttc taaacaatcg ttacttctac atcgatattt
     6241 cctctagttg ctttggagta agggcagaat tcatgcgcat cttctgcaat tttttgggct
     6301 tcttcttcgc tgatgccatc gatatgcact tctaatacta caccgatctg atagccgcca
     6361 tcatctaaat tataaagaga gacacgtgct tttactgtgg actctgctgt cattcctgct
     6421 tctttgatca tatgttgtac cgccccattg aaacaagaag cataacctgc tgcaaataat
     6481 tgttctggat ttgttccttc tgcatggatt cctggaggag tgattttcat atgcatcgag
     6541 ccatttggtg cctcgacttc tccattgcgc ccgccatggt tgatcattgc tgtttcgtat
     6601 gcttttttca ttgtactgct cctttcgatt gaccatttgc tacttggcta taattatagt
     6661 gtatgacaag taaggcgctt tctacaaaaa ttctgcttat tcgaatacca gaatgccgtc
     6721 ttctactatt tttccaaaaa acaaaaaaat actactcgtg aacaacagcg tcatatttgt
     6781 tccaaaataa atagcaatct tttctacata atcttttata tgattgattt taagatacat
     6841 atataccaga aagttgaccc cataaaccaa tactgttaaa aaaataacca tattcgttac
     6901 cattttttcc tccttttgct ggtctcccta ccagcactcg atttaaccat accacttttt
     6961 ttgccgtcca agcaactagc ctttcaaaaa gactggttta ttctgctgtt aatgattcaa
     7021 gatcagaaaa atgtccgaat aattttgcta attttactaa cgccaacttg ttctttccga
     7081 attcatcatt ttctatctgt aatactagta acggctcatg taagcttaaa cggatcaaaa
     7141 accagccgtt tccgtatggc tcttctagat catatcgaat accttcttca ttttccgggt
     7201 cgaagttcat ccctttttct tcttccaaca gtcgcttcat ttcttctaat atcttgtttc
     7261 cttgttcgcg tggttcatcg ctgaacaatt ttaatcggat ttcttgtact tctacgggct
     7321 gtttcaactc tttgatttgt tcatctaatg tgataccttt tctttttaat tctggcaaaa
     7381 gcatcaggat ctttgcgatg acatatgcac catcatccag aaaatagttt tccttgaatg
     7441 cagcgtgtcc gcttgtttcg atggctagct gtgtatcttt cccaagacgg ttaagttcta
     7501 atgctttatt gattacattt cgatagccgg aaatataacg aacttggatg cctccttttt
     7561 cttcaatgaa tctttgtaga tgagaagaag taggggaatt cgtcacgatt cttgttcctg
     7621 gatgctcttt taatacgatt gtacttaata ctgcgatcaa attatttcga ttgatcactg
     7681 ttccagaagc actgacaact gcagaacgat ccacatctgt atcgaaaatg atcccaagat
     7741 ctgcttcatt tgctaaaaca gcctgcttga tactttccat cgcttcttta ttatcaggat
     7801 ttgggatatg attaggaaat cggccatctg gttccaaaaa ttgggaacct gttgaatccg
     7861 cacctaagct attcaatact ttttcagcaa agaatccacc agcaccattt ccggcatcaa
     7921 caatgatttt ccaaccattt agtggtttgc tttctagtgt tccaatcccc tttttaattt
     7981 ttgtgactaa atcttgcgca taaggtgtca aaaggtctgc tttttctacg cttccagttt
     8041 gttcgctttt tattttttgc tgctgtgata ggataaaagc aatatcttct ttttctgctc
     8101 ctccttctga ggtaaagaat tttattccat tataataata gggaagatgg ctagcagtca
     8161 gcataaccgc agcatcgcac gagaactgtt caaattgagt ggacataaac atagcaggcg
     8221 tagtagctaa ttcgaaatcg atgacatgta tcccttgttc tttcagactt tggatcaacg
     8281 cctctttcag tgctggtccg cttatcctac tgtcatgacc aatcccaatc gtccattttt
     8341 tatttggatc ggttgatttc ttctttttct ttatccagtt tgctagtccc cagccgatct
     8401 tttttaccat gtctgtcgtc aagtttactt catatccttc aactggtaat gcgattcctc
     8461 gaatatccga tccattttgc agcgctttca tatctttcat ataaaagtcc tcctcattta
     8521 ttccctcatt ttttccagct tttctgcaaa tcgctgtgct tcttcttctg agcggcaaat
     8581 aataatgaat gtatctacac cggccatcgt tccgacaact tcagggtaag ccacttcatc
     8641 tagccaattc gttaccacat cggcaccatt tcgttcagtc aatacgataa ccatgaactg
     8701 aacagactgg atacgcaaaa cctcgcgtct aattgctgta cgcaaacgtt cttcatttaa
     8761 agaaacagat ggctggttaa ataatgtata gcgtactatt ttgttttcat tttgactttt
     8821 tactaacttc atttctcgaa tgtctcgaga aatcgttgct tgagtcacat cggcgcctgc
     8881 ttcatgcagc cgcgcaagca gctcttcctg tgtcccaata ggttgttctg taatgatctg
     8941 gcggatcagt gcttgtcgct gtttcttttt catattagac acctctcaat gttgattata
     9001 gcaaaagata agtaatattt cttcatattt tggtattttt tacttttatt ttttgttttc
     9061 attcaggttg ctaacctttt tctattttaa cacgcaaaat cgtcttcatt ttttcaggtg
     9121 ctgattttct tggatcgctg atgtaaatct ctttgtgttc tttagaagct ctttcatagc
     9181 cttcttttgc gatgaatgct tctaatttct caaacgttgc tggttcttcg gcatagggcc
     9241 cgacgtgtaa gatttgggca caaagacctt cttccatctt tttgaatact acttgttctt
     9301 tcaagtcttc cggaagtttg ctagaacgct gaatggcttc ttgtgctatc gcttctgtaa
     9361 caaagtcagg ctgtcggatc atcaattgat aagaaaaatg ttcttttttc atcactggtt
     9421 catgcaaata cttttcttgc agtccccatt gtccttccag tggatagaca gtatacatct
     9481 gataatcagg aatcagccaa ttttttttag gcgacatgcg tattgcatag ctgactgcat
     9541 atagacaggc tactcttcgt ttaaagtctt cctgattcgg gtcgccgatc cctctgatgc
     9601 taaagaatga ttttttagga acctctagta tttgcgggct ttttttcata ctatagctct
     9661 tgtctttacg ccattcaatt ttttccattt ttcttcctcc gcgaaattcc atttagttat
     9721 ttattatcat acctcttttt gagtgacttg gctaatcgtg ttgacactat ttttcagttt
     9781 gtgctagaat aagtgaagta aataaatcgt acagataaca cgtttcggcg tgtcgagaga
     9841 gaggcggttt ctatttttat aatagaaatc actctctttt tttacgaaat tttttagagg
     9901 tgatgtaaat gacaaaacaa aaacagctgc ttttagtacc tgcagtaatt gcaacaggga
     9961 tgatgtcttt tgcgggcgta ttgatcgaga cggccatgaa tgtaactttc ccaactctta
    10021 tcagacagtt tgatttaacg actgcacaag tccaatgggt aacaacgatt tatcttttga
    10081 tgatttcgat cattgttccg ctgtctactt atttgaatcg caattttagt ctcaaaaaac
    10141 tctttttagc ttcaaatctg cttttcttgg caggtataat gatcgatttc ttctctccta
    10201 ccttcatggt cctattgttt ggtcgattgc tccaaggtgc agcgacaggt atcgctcttc
    10261 cgttgatgtt ccatattatc ttgacatttg cccccctcaa tcgacgaggc gcgatgatgg
    10321 gtgtcggtac tttgacaacg gccattgctc cagcaattgg ccccacttat ggaggcatta
    10381 tgacttcaca ttttacatgg aatcatatct tcctgtttct tgtgcccgtt ttgtttcttt
    10441 ctttgattat cggctggttt gctattcccg agatggcagt acaaaaaagt ggttatttgg
    10501 attggataag cgttttagga atttgtcttt tgtttagtgg aagtctaaca tttttgagta
    10561 cgttgggaac agtttccagc tggattgctt taatcatcgg cggtattggc tggttccttt
    10621 tttactatcg aagtaaaaaa gtggagcatc cactgatccg actgtctatt ctttcaaata
    10681 aaacattccg cttgtttttg attggcttct tagtttatca gtttttatta cttggtgtat
    10741 cttttgtctt gccgaatttc cttcagatcg ttcaaggaag tagtcctttt gtcgctggat
    10801 tagcgatgct tcccggcgca gcggttggtg ctttgctttc tcctttttcc gggaaaatgc
    10861 tggatcaata tggtccgaag aagccaattt ttgctgggct gattctttct ttgattggtt
    10921 ggctagcatt attcctactc ataggacacg cttcagtggg tgtattgatt gcttgtcatg
    10981 tgttttatat gatcggtatc ggattatctt acagcaatat gatgaccacc ggaatgaatg
    11041 cactgaatga agaactacaa ggagacggga acgcaatgtt caataccttg cagcagtttt
    11101 ctggcgctgt ggctacttca ttagtagcgg tcattatcaa ttacgtccag catcatactt
    11161 cccataacta tgaagtgtct actacattag gatcaaaagc agcattaggc gtcttgttgc
    11221 tgttgcttct cgtaagcttt gctcgattcg cctattatct gttctttgct aaaaaagcgt
    11281 agattccata tacggcaggt gttgcatctg ccgttttttt gtgtgaaaat ggttgtttgt
    11341 aaaaggaggc ttttttatgg aaaaaattgc cattatcggt ggcgggatca ttggtatgac
    11401 cctagccagt tacttagata catcaaaatt caatgttgct ttatttgatg aagggacagg
    11461 acaggcaact aaagctagcg ctgggattat ctccccttgg ttgtcgaaac gcagaaacaa
    11521 acaatggtat cgtctggcta aagacggcgc tgcttttttc cctaaactta taaaagattt
    11581 ccaacttgat caaacgattt acgcacaatc agggacactt ttgcttcgac ctgaaaaaga
    11641 tttagctgaa ttggctgttc ttgccgagaa acgaaaagaa gaagcgccag aaattggaaa
    11701 catcgaatgg ctttcacctg aacagacatc tcaagcactt ccgttattga aaccagcagt
    11761 ttcactgcgt atcagtggtg gtggtcgtct ggatggaaaa gcatatttag aacatcttgg
    11821 tcagcgttta gcacaaaaac aaatggagca gatccaggca aaagtcactt tgcataagaa
    11881 gagcgattct tggctcatca agggaccgtt tggagaatac acagctgatc gtgtaatttt
    11941 gacacctggc ccggcactta agcctttatt acaagaatta ggctatgctg cagatatacg
    12001 tccgcaaaaa gggcagctct tagcatttca gacatcttat aagatgagtc aagactggcc
    12061 tgttgctatg cttgacggag aagctgattt cattcctttc atggatggaa agatcctttt
    12121 aggtgctacc catgaaaacg atcaaggctg ggatttggct cctactagcg aagcttataa
    12181 gggattgact aagagtacag aacagttctt gactgatcca gatcagataa aaaactggcc
    12241 atacgagttc cgtgtaggta cccgtgctta tacgtcagat ttttccccat ttttcggcgt
    12301 actgccagat gatccaagct tactggctgc tagcggtcta ggctcttctg gcttgaccac
    12361 aggtccttat atcggctatc tattagccaa cttcttaaat actggtcatt ggcctacgga
    12421 agattatcaa aaggacatct ccacctatat tacgaaaaat ccatcataga aaaacagctt
    12481 tttcaagtaa ggatcccgag cgatattgcg catggattac ttcttgaaaa agctgttatt
    12541 ttagttcaat ttctgaatca aattctccaa actttctttt gcttcatctg gcaagagatc
    12601 ttttcctatc tgagcttcaa tgaatcgtaa acgaatatca agtcgttgtt tcaccagatt
    12661 tttataggac gtatcctcaa aagaaacagg aaaatctggt ataggtaaat agtggatgtc
    12721 ttcaaatgga ccaaacgact tgaattctgc cgtttgttgc acgattcttt caacgattga
    12781 caatgtcgtt tcaggtttga tttttttatc atcgaaatat ccagtattca ggacatagca
    12841 ggctgtttgc tgtttcaaaa acaactctcg aaaagcttga tagtcttccc taagaggata
    12901 gacacggaaa ggattagcaa atggttcaat gactagctca tctagctgtc tttttgtagt
    12961 gatattttcc gcgtctgatc gttttgttgc taatgttgca ccaaaagacg ctgctaaaac
    13021 tgggtcattg atcttgataa ttggcggaag cgaatcatct ttcatgatcc aaaaaacagc
    13081 atcgatcttt tcgctaagat gatcgactcg gtcgggtgtc gcataacgtg atttgactgt
    13141 ccgcccattt ccgtttcgta tatctcctgt tactaggact ttttctcctt tacttgtaca
    13201 tgtcacgccc acattctgac aagtaaggaa agaagaaatg gccggatcat cagacggata
    13261 gtcttgtgtt ttatcaaagt aagaaggttc caaggcagta gcaaaaccat tttttcgaga
    13321 aataacgaaa gcatcgtcat gcaagatcga aacagagtga ccttccgtat gttttgctaa
    13381 ggtaatcgtt gattttccag aaccggacaa cccaaataca gccatcgtat aggtctggtc
    13441 attactagca tattgtttca tcccaccgtg gcaggcaata aagccattgc gatttcccaa
    13501 tgcccaagca agcgtcaaag tcgctttttt taattctcca aagtaacgta atcctagcac
    13561 agccgctgta ttatggagag catcaaacaa aatgagtcca tttggaaatg tttcatgtgt
    13621 ccattcggga tcacaaaaga tataaagatc tccctcatca aaggaaacag acttttgata
    13681 agtctctaag atcttcgaat tcgctggctg aaaattcagc aaataagaat acaaattgtt
    13741 ctcaaatcct tctggaatca gcaaatgact ttgcacagaa aattcttcag ataggccaac
    13801 gattactttt gtatggtaaa actggcgctt gcgactttga tacacagctt ctcttaaaat
    13861 tttctcgtat ttgctttgtt ccttgtctaa gtcctcaaag atccgccgag cacttgctgt
    13921 tcttcccaca atgcttccat cattgaatac tagtgtccta acatcagctg gtaaatctag
    13981 ttcttcagca tgtgagattg ttaaatctgt gatgatcgtt cctggactcc cttttgctag
    14041 ttgataagct gtcttcctat caaaaacttc atgaacatta ttaccgtaaa atgtcgtttc
    14101 tacaatcgat cgaatactgg ataacagtgg atttccactt gtgatttcgt ttaattcgag
    14161 actctctttt gtactcatgg ctcctcctca ttccctaaac aaaaaaacgt tttctttatt
    14221 atacattatt cgagttttta tttgtactgc tgcgctgtgg gaagcaaacg agttattttt
    14281 cacacaaaaa aaccgagaac ttgaataaac aagtttctcg gtttcatcat tttcctttta
    14341 tcggcttagt accagccgtt tgctaaccag aatgttttag cagcatccca tgaaccataa
    14401 cgagatgtta catattgttc tgctacacgt tcttgattgg cagctgaata gtcgccgttt
    14461 aagtatgaag catctaattg ataacggccg atgtaacgtc cgttagtagc tgtgtatgaa
    14521 ccgcctgatt ctttttgtgc gatccattct tttgcagagc ttgaagttgg cgctgttgca
    14581 gcagctgggg cagcaggagc tacttcaact gtttcaacag gtgcaacgta tgtatgttct
    14641 tgcgttgctg tttcagttgt agatgctgta ttttctgttg ttttgttttc tgttgttgct
    14701 gaagaatcat ttgaagttgg gattgttaat tgttcaccaa cataaatgcg gttgatgttt
    14761 gcgatcgaat ttttttcagc aattgcatcg atcattgagt tgtctcctgc aaatttttgt
    14821 gagatcttag ataatgaatc acctgatttt actgtataaa cttcatcagc gtgagcattt
    14881 gtagttccta caaacattgc agtagcacca gcagcaaaag ttgtacctaa aacaattttc
    14941 ttaaatgaat tcatattaaa ctcctttttt tctccgtttt ttattctttc gacgctatta
    15001 act
//
LOCUS       DAYYGO010000068        14789 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000068 DAYYGO010000000
VERSION     DAYYGO010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.070"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            125..1084
                     /locus_tag="ACHA5T_001805"
     CDS             125..1084
                     /locus_tag="ACHA5T_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFM8887374.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIFDIDTLPMRGKRKPNGHQEKRGKQQYQRSIHDRSDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRK
                     VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            1406..1576
                     /locus_tag="ACHA5T_001806"
     CDS             1406..1576
                     /locus_tag="ACHA5T_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887375.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            1590..2192
                     /locus_tag="ACHA5T_001807"
     CDS             1590..2192
                     /locus_tag="ACHA5T_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8887376.1"
                     /translation="MAKIGYARVSSKEQNLDRQLKALEGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVVVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINNLVIELYKYQAESERKRIKERQAQGIEIAKKKGKFKGRQPKFKENDPRLQHAF
                     DLFLNGCSDKEVEEQTGINRRTFRRYRSRYNVTVDQRKKQ"
     gene            2208..2885
                     /locus_tag="ACHA5T_001808"
                     /pseudo
     CDS             2208..2885
                     /locus_tag="ACHA5T_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            3196..3693
                     /locus_tag="ACHA5T_001809"
     CDS             3196..3693
                     /locus_tag="ACHA5T_001809"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="HFM8887377.1"
                     /translation="MLFLRDGEIKATLTTMMNKLAFSHKLILEPLFKSVSQIDEESDR
                     ERMDAIDKLMEQLLEERNTLIALMSKGFLEPALFNQERNVLDSEIKNLTTEKTNLVTN
                     SASGVLRANEIKDLINYVSADNFNGDYTEELFEEFVVNIIVNSRDELTFNLKCGLSLK
                     EKVVR"
     gene            3694..4110
                     /locus_tag="ACHA5T_001810"
     CDS             3694..4110
                     /locus_tag="ACHA5T_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HFM8887378.1"
                     /translation="MAYIPYGYKIQDGVVTVDEKAAGQVKVFFEKYISGLSLTVAGEQ
                     AGIEKTHSVMGRILKNVNYLGNDTYPAIIDKEIFDKAEEVRDKRAKDLGRVVELAAFT
                     SPPPKERFKMKKADNKMPVDPFERAEYLYSLIESEE"
     gene            4130..5674
                     /locus_tag="ACHA5T_001811"
     CDS             4130..5674
                     /locus_tag="ACHA5T_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8887379.1"
                     /translation="MVIPARKRVGSTAAKEKIKKLRVAAYCRVSTETEEQNSSYEVQV
                     SHYTEFIKKNNEWEFAGIFADDGISGTNTKKRDEFNRMIAECMDGNIDMVITKSISRF
                     ARNTLDCLQYIRQLKDKNISVYFEKENINTTDAKGEVLLTIMASLAQQESQSLSQNVK
                     LGLQYRYQQGKVQVNHKRFMGYTKDEDGNLTIVPEEAEIIKRIYREYLEGQSLVGIGR
                     ALEKDGILTAAGKPRWRPESVKKILQNEKYIGDALLQKTVTVDFLTKKRVKNEGHVPQ
                     YYVENSHEAIIPKELFLQAQEEIHRRSNIYTGEGKNKRIYSSKYALSAITFCGDCGDI
                     YRRVYWNIHGRKEFVWRCVTRIEQGPEVCKNRTVKEDELYGAVMTAINKLLAGGNNMI
                     KTLEENIHAVIGETTEYQISEINNSLEEKQKELIKLANKGQDYDHLADEIDELRDKRQ
                     ILLVEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEGHFVIAFKS
                     GIEMEI"
     gene            5817..6041
                     /locus_tag="ACHA5T_001812"
     CDS             5817..6041
                     /locus_tag="ACHA5T_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8887380.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            6056..6925
                     /locus_tag="ACHA5T_001813"
     CDS             6056..6925
                     /locus_tag="ACHA5T_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFM8887381.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            6906..7640
                     /locus_tag="ACHA5T_001814"
     CDS             6906..7640
                     /locus_tag="ACHA5T_001814"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8887382.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            7673..8581
                     /locus_tag="ACHA5T_001815"
     CDS             7673..8581
                     /locus_tag="ACHA5T_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFM8887383.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            8578..9120
                     /gene="sat4"
                     /locus_tag="ACHA5T_001816"
     CDS             8578..9120
                     /gene="sat4"
                     /locus_tag="ACHA5T_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HFM8887384.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            9213..10007
                     /locus_tag="ACHA5T_001817"
                     /note="aph(3')-IIIa"
     CDS             9213..10007
                     /locus_tag="ACHA5T_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFM8887385.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            10283..10414
                     /locus_tag="ACHA5T_001818"
                     /pseudo
     CDS             10283..10414
                     /locus_tag="ACHA5T_001818"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
     gene            10490..10983
                     /locus_tag="ACHA5T_001819"
                     /pseudo
     CDS             10490..10983
                     /locus_tag="ACHA5T_001819"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            11153..12049
                     /locus_tag="ACHA5T_001820"
     CDS             11153..12049
                     /locus_tag="ACHA5T_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8887386.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMIERIIQLENEKE"
     gene            12148..12357
                     /locus_tag="ACHA5T_001821"
     CDS             12148..12357
                     /locus_tag="ACHA5T_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFM8887387.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            12374..12646
                     /locus_tag="ACHA5T_001822"
     CDS             12374..12646
                     /locus_tag="ACHA5T_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFM8887388.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            12648..13511
                     /locus_tag="ACHA5T_001823"
     CDS             12648..13511
                     /locus_tag="ACHA5T_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFM8887389.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(13774..14511)
                     /gene="erm(B)"
                     /locus_tag="ACHA5T_001824"
     CDS             complement(13774..14511)
                     /gene="erm(B)"
                     /locus_tag="ACHA5T_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFM8887390.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(14636..14719)
                     /locus_tag="ACHA5T_001825"
     CDS             complement(14636..14719)
                     /locus_tag="ACHA5T_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFM8887391.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
BASE COUNT         5246 a         2326 c         3038 g         4179 t
ORIGIN      
        1 ccgcactatg ccattacatt tatatctacg cgaattgtaa aattaagtta gaaaaataaa
       61 aaggcattta tggtacactc aaattgtatt tccgacgaaa gaaaacaaag gagtgtaacc
      121 ataaatgacc tacaaacatc ttaccataga cgaactgaca atgatagaat catattatct
      181 tcaacataat aagccggttg aaatcgctaa ccgaatggga cgtgctatac aaactattta
      241 taatgtagtc aataagttca agcaaggcaa gactgctctt gattattggc accagtataa
      301 agaaaataag aaaaaatgtg gtagaaaagt cattcaatta cctgctcatg aagtagatta
      361 cattaaagag aaagtcactc ttggttggac gcctgacgtc attatcgggc gaaaagaaag
      421 gtctgtttca tgcggtatga gaacacttta tcgtttattt tctaaaggaa tatttgatat
      481 tgacacacta ccgatgagag gtaaaagaaa acccaatggc catcaggaaa aacggggaaa
      541 acaacagtat cagcgctcaa tccatgatcg atctgataat tatcctgatt tcaattctga
      601 gtttggtcac cttgaaggtg atacgatcgt tggcattcat cataaaagtg ccgtcattac
      661 tttagttgaa agattatcta aagtcattat cacgattaaa cccaacggcc gtaaggcatt
      721 agatattgaa actgccctta atcaatggtt ttctcgcttc cctaaaaact tctttaaatc
      781 tattacgttt gactgtggaa aagaattttc taactggaaa gccattagta accaacatga
      841 tattgatata tattttgcgg accctggaac accttctcaa cgcccattaa acgagaattc
      901 taacgggatt ctgcgtcgta atggactgcc gaaatcaatg gattttagaa aagtgaatca
      961 gacatttatt tccagtgtca gcaatcaacg taatcatatt ccaagaaaat cattgaatta
     1021 cagaacacca atcgaaatat ttttgagcta tgtacaagaa gcattttatt ctagcttaat
     1081 ttgacaaatc atactatgat acgtgtttgt ttttctgtgc tgtttagtga atgattagca
     1141 gaaatataga gagtaagatt ttaattaatt attaggggga gaaggagaga gtagcccgaa
     1201 aacttttagt tggcttggac tgaacgaagt gagggaaagg ctactaaaac gtcgaggggc
     1261 agtgagagcg aagcgaacac ttgatctttt aagttgctat ctttttaagg tcaatagagt
     1321 atacttattt gtcctaatat ggttttagca gtataattga cttggtgaat aggtcattta
     1381 agttgggcat aataggagga gtaaaatgaa aaaatttatt tatcgagttt tagaaaatga
     1441 cgaagtggtg gctattttta atgagcaaca atatgcgcaa gattttatcg cttacgaaaa
     1501 gacaatttct gataagcaat ttgaaattga aaaagtagat atagctgatt ggttattgca
     1561 accgagagaa ttttagaggt tggttgaaaa tggctaaaat tggttatgca cgtgtcagta
     1621 gcaaagaaca gaacttagat agacaactga aagcgttaga gggcgtttct aaggtctttt
     1681 cagacaaatt aagcggtcaa tcggtcgaac gcccacaatt acaagctatg cttaactata
     1741 ttcgtgaagg ggatatagtt gttgttactg aattagatcg attaggacga aataataaag
     1801 aattaacaga attgatgaat caaattcaaa ttaagggggc aaccctggaa gtcttaaatt
     1861 taccctcaat gaatggtatt gaagatgaaa atttaagacg gctgattaat aatttagtga
     1921 ttgaattgta taagtaccaa gcggaatctg aacgcaaacg aattaaagaa cgccaagccc
     1981 aaggaattga aattgctaag aaaaaaggaa aattcaaagg gcgacaacct aaattcaaag
     2041 aaaatgatcc acgtttacaa cacgctttcg atttattttt gaatggttgt tccgataaag
     2101 aagttgaaga acaaactgga attaatcgcc gaacgtttag aaggtatcga tcaagataca
     2161 acgtgacagt agatcaaaga aaaaaacaat gaaaagaggg atagttaatg agtacggtta
     2221 ttttagctga aaaaccaagc caggcattag cctacgcaag tgctttaaaa caaagcacca
     2281 aaaaagacgg ttattttgag atcaaagacc cactatttac agatgaaacg tttatcacct
     2341 ttggttttgg gcatttagtc gaattagcag aaccaggtca ttataacgaa aagtggcaga
     2401 actggaaact tgaatctttg ccgatttttc ctgatcgata cgattttgaa gttgcaaaag
     2461 ataagggaaa gcagtttaaa attgttgctg aacttctcaa aaaggcaaat acaattattg
     2521 tcgcaacaga tagcgacaga gaaggtgaaa atatcgcctg gtcgattatc cataaagcaa
     2581 atgccttttc taaagataaa acgtataaaa gattatggat caatagctta gaaaaagatg
     2641 tgatccgaag cggttttcaa aatttgcaac caggaatgaa ttactatccc ttttatcaag
     2701 aagcgcaaac acgccaaatt gccgattggt tgatcggcat gaacgcaagc cctttatata
     2761 ccttgaattt acaacaaaaa ggcgtacaag gtacattttc attaggacgt gtacaaacac
     2821 cgactttata tctcatttat caacgccagg aagcaataga aaactttaaa aaagaacctt
     2881 ttttcctgaa taattcatag ttttactaat agtctttcct aacattgatt taatagtctt
     2941 taaagtattc tgcttcatca cccattaggt gatgaaaaaa gaaccccttt ctgatttagc
     3001 ggacaaatac tttgacctaa tctcagaaga tataaaagaa aaaataataa aatatccgat
     3061 ttatactttg tataaagaac taaagtagtt agcaaatcat ttacagcaaa tcactatgtt
     3121 ccacatacca ttttacatac ttaccttatc cacgacacat gttgagatag tatcgaagat
     3181 aaagaaagtt gctccatgtt gtttttgcga gatggggaaa tcaaagccac attgacaacc
     3241 atgatgaata agcttgcttt cagtcataag ctaatcctag aaccactttt caagtcagtt
     3301 agccaaatcg atgaagaaag cgaccgtgaa agaatggacg ctattgataa gctaatggag
     3361 caactattgg aagaacgcaa tacccttatt gcactgatgt ccaaaggttt cctagaacct
     3421 gctcttttta atcaggaacg aaatgtttta gatagtgaga ttaaaaatct tactaccgaa
     3481 aaaacaaacc tggtaacgaa ctctgcgagt ggggttttgc gagcaaacga gataaaggac
     3541 ctcattaatt atgtgtcagc agataatttt aatggtgact acacggaaga actatttgaa
     3601 gaatttgtag tgaacatcat tgtaaattcc agggatgagc tgacattcaa tttgaaatgc
     3661 ggtctttccc tgaaagaaaa ggtggtgaga taaatggcat atattccata tggatacaaa
     3721 attcaagatg gagtggttac tgtcgatgaa aaggcagcag gtcaagtaaa ggtattcttt
     3781 gagaaataca tatcaggact atcccttaca gtggctggcg aacaggcagg tattgaaaag
     3841 acacattctg tgatggggcg cattttgaaa aacgtcaact accttggaaa tgatacgtat
     3901 ccagcaatta ttgataaaga gatatttgat aaagctgaag aagttagaga taaacgtgca
     3961 aaggatttag gacgagtggt agagcttgcc gctttcacct ctccccctcc caaagaacga
     4021 ttcaaaatga aaaaggcaga taataagatg ccagtggatc cttttgaacg agcagaatac
     4081 ttatacagtc tgatagaaag cgaggaataa agtgacagag aaaaatataa tggttattcc
     4141 tgctcgtaaa agagtaggaa gtacagccgc aaaagaaaag ataaagaaac ttcgtgttgc
     4201 tgcctattgc cgtgtttcta cagaaacaga agaacaaaat tctagctatg aggttcaggt
     4261 ctcacactat acagagttta taaagaaaaa taatgaatgg gagtttgctg gcatctttgc
     4321 agatgatggt atctctggta cgaacactaa aaagcgtgac gaatttaatc gcatgattgc
     4381 agagtgtatg gacggtaaca tcgacatggt tattacaaaa tccatcagcc gatttgcgcg
     4441 taacacccta gactgccttc aatacattag acagctcaag gataagaaca tatcagttta
     4501 ttttgagaaa gagaacatca acaccacgga tgccaaaggt gaggttttgc tgactattat
     4561 ggcatctttg gcacagcagg aaagccagag cctttcacaa aacgttaagc ttgggctaca
     4621 gtaccgatac caacaaggaa aggtgcaggt caaccataag cgatttatgg gctacacgaa
     4681 agatgaagat ggaaatttaa ctattgtccc cgaagaagct gagattatca aacgcatcta
     4741 ccgagaatac cttgagggtc agagtctagt gggcattggt cgagcccttg aaaaggatgg
     4801 tattttaaca gcagcgggaa aaccaagatg gcgaccagaa tcagttaaga agatacttca
     4861 aaacgaaaaa tacatcggag atgcccttct gcaaaagact gtcacagtag attttctgac
     4921 caagaaacga gtgaaaaatg aagggcatgt tccccaatat tatgttgaaa atagccatga
     4981 agcgattatt cctaaagagt tatttttgca ggcgcaagaa gaaattcatc ggagaagcaa
     5041 tatctacaca ggagaaggca agaacaaaag aatttacagt agcaagtacg ctttaagtgc
     5101 catcaccttc tgtggagatt gtggcgatat ttatagacgg gtctattgga atatccacgg
     5161 aagaaaagaa tttgtctggc gatgcgtgac tagaatcgag caaggtcctg aagtttgtaa
     5221 gaaccgaacc gtaaaagaag atgaacttta tggtgcagta atgactgcga ttaataagct
     5281 acttgcaggt gggaacaata tgataaagac actggaagag aatattcatg ctgtgattgg
     5341 cgaaaccaca gaataccaaa tttcagagat taacaactca ctagaggaaa aacaaaaaga
     5401 actaatcaag ctggcgaaca agggtcaaga ctatgaccac ttagcagatg agattgatga
     5461 gctgagagac aagcgacaga tccttttagt agaagatgcc tccctcagtg gcgagaacga
     5521 gcgaatcaat gagctgattg aatttatccg caagaacaaa ttccgcacct tagaatatga
     5581 tgataagctt gtaaggaaga taatccagag cgttacagtc tatgaaggcc acttcgtcat
     5641 agccttcaaa tctggcatcg aaatggaaat atgaaaacca gaagtaaata gcccatgact
     5701 ctacagtaga gttgtgggtt ttcttttgct tgctgattat aataatattt taataactat
     5761 tgtgtttatc aaccaaatgg tttataatat tcttagcgta aacttggagg tgctgtatga
     5821 ctacggcaga aatgattaaa gaactgtgtg agcaaatgaa tataagtgtt tccgaacttg
     5881 ctagacgtat tggccagact ccacagaatt tcaataaaaa attacaacga gaaacagtaa
     5941 ccttggatga gttgaaggcc attgctgatg tgctaggtgt caagtttgtg caggcattta
     6001 ttttaccaga tggagaagaa ataaaaatat ctaacgaata aaggaggatg tttatatggt
     6061 aggtaatgtt attcaaatag taacagagaa attatcatct ttgcctttta tagaaggcat
     6121 cgtattaggg ggctcacgtg caagaggcac ccatacagaa aattctgata tagatatcgg
     6181 aatctattac aattcagact catttgacct gacagcgatt aatcaaattg ctacagagtt
     6241 ggatgatgag aatagaaaca accttgttgt acctcccgga gcgtggggtg attgggttaa
     6301 tggcggtgga tggttagtta taaacgggtg tcatgttgac ttgatattac gtgatataaa
     6361 acgggtggaa caaataatca aagatacgga gcaaggaatt gttactgcca attatcagac
     6421 cgggcatccc catggttata taagtgctat gtatcgtgga gaattagcga ttagtaaaat
     6481 actatatgct aagaacgaaa gcttatgcga attaaaaaag caggcagaaa tttaccctac
     6541 tgctctaaag aaaagcttga tgaacttttt tatatttgaa gctgaattct ctttaatgtt
     6601 tgtaaaagca aatgcggggg cagaggataa atattatatt gcaggccatg tttttcgtat
     6661 tatttcatgc ttaaatcaag tactatttgc atgtaataat gcttattgca ttaacgaaaa
     6721 gaaagctata aaactgcttg aaacttttga atataaacct gaaaaatatg ccgaaagagt
     6781 taatcatatt tttgaagtac tcggtctttc tctttttgaa tgctacgaca tgaccgagaa
     6841 actttataaa gaagtgaatg aaattgtatc ggagataaat aactttttaa acgaggagag
     6901 ttcagatgaa agaaaacaaa tatgatgata atatattttt tcaaaaatac agtcaaatga
     6961 gtcgctcgca gaaaggactg gctggtgcgg gagaatggga gactttgaaa aagatgctac
     7021 ctgattttaa gggtaagcgt gtgcttgatt taggatgcgg ctatggatgg cactgtatat
     7081 atgcgatgga aaacggtgct tcctctgtag taggtgttga tatttctcat aaaatgctcg
     7141 aagtagcaaa aggaaaaacc cattttccac agattgaata tgaatgctgt gccatagaag
     7201 atgtggattt cccagaggag agctttgatg taatactaag ttcgcttgcg tttcattatg
     7261 tagcagacta tgagaattta ataaaaaaga tatataggat gctgaaggct ggtggcaatt
     7321 tagtttttac agttgaacat cctgttttta ctgctcatgg aacacaagac tggtattata
     7381 acgaaaaagg agaaatactg catttcccgg tggacaatta ttattatgag ggcaaacgga
     7441 cagctatgtt tttggaagaa aaggttacaa aatatcatag aacactgacc acatatctaa
     7501 atacactgct ttcaaatagt tttataataa atcagattgt ggagccacag ccgccagaga
     7561 acatgatgga tattccgggg atggcggatg aaatgcgacg cccaatgatg ctgattgtat
     7621 cggcaaaaaa gaagatgtaa taatatagaa aaaataaacg aggagtatgt aaatgagatc
     7681 agaaaaagaa atgatggatt tagtactttc tttagcagaa caggatgaac gtattcgaat
     7741 tgtgaccctt gaggggtcac gcgcaaatat taatatacct aaagatgaat ttcaggatta
     7801 tgatattaca tattttgtaa gtgatataga accgtttata tctaatgatg actggcttaa
     7861 tcaatttggg aatataataa tgatgcaaaa gccggaggat atggaattat tcccacctga
     7921 agaaaaggga ttttcctatc ttatgctatt tgatgattac aataaaattg atcttacctt
     7981 attgcccttg gaagagttag ataattacct aaagggcgat aaattaataa aggttctaat
     8041 tgataaagat tgtagaatta aaagggacat agttccgact gatatagatt atcatgtaag
     8101 aaagccaagc gcaagggagt atgatgattg ctgcaatgaa ttttggaatg taacacctta
     8161 tgttattaaa ggattgtgcc gtaaggaaat tttatttgct attgatcatt ttaatcagat
     8221 tgttcgccat gagctgctga gaatgatatc atggaaggtc ggcatcgaaa caggctttaa
     8281 attaagtgta ggcaagaact ataagtttat tgaaaggtat atatccgagg atttgtggga
     8341 gaaacttttg tccacctacc ggatggattc ctatgaaaac atatgggaag cattatttct
     8401 atgccatcaa ttgttcaggg cggtatccgg tgaggtggcg gaaaggcttc attatgccta
     8461 tccggagtat gataggaata taacaaaata taccagggac atgtataaaa aatacactgg
     8521 taaaaccggc tgcctggata gcacatatgc cgctgatata gaagagaggc gggaacagtg
     8581 attacagaaa tgaaagcagg gcacctgaaa gatatcgata aacccagcga accatttgag
     8641 gtgataggta agattatacc gaggtatgaa aacgagaatt ggacctttac agaattactc
     8701 tatgaagcgc catatttaaa aagctaccaa gacgaagagg atgaagagga tgaggaggca
     8761 gattgccttg aatatattga caatactgat aagataatat atctttacta ccaagacgat
     8821 aaatgcgtcg gaaaagttaa actgcgaaaa aattggaacc ggtacgctta tatagaagat
     8881 atcgccgtat gtaaggattt cagggggcaa ggcataggca gcgcgcttat caatatatct
     8941 atagaatggg caaagcataa aaacttgcat ggactaatgc ttgaaaccca ggacaataac
     9001 cttatagctt gtaaattcta tcataattgt ggtttcaaaa tcggctccgt cgatactatg
     9061 ttatacgcca actttgaaaa caactttgaa aaagctgttt tctggtattt aaggttttag
     9121 aatgcaagga acagtgaatt ggagttcgtc ttgttataat tagcttcttg gggtatcttt
     9181 aaatactgta gaaaagagga aggaaataat aaatggctaa aatgagaata tcaccggaat
     9241 tgaaaaaact gatcgaaaaa taccgctgcg taaaagatac ggaaggaatg tctcctgcta
     9301 aggtatataa gctggtggga gaaaatgaaa acctatattt aaaaatgacg gacagccggt
     9361 ataaagggac cacctatgat gtggaacggg aaaaggacat gatgctatgg ctggaaggaa
     9421 agctgcctgt tccaaaggtc ctgcactttg aacggcatga tggctggagc aatctgctca
     9481 tgagtgaggc cgatggcgtc ctttgctcgg aagagtatga agatgaacaa agccctgaaa
     9541 agattatcga gctgtatgcg gagtgcatca ggctctttca ctccatcgac atatcggatt
     9601 gtccctatac gaatagctta gacagccgct tagccgaatt ggattactta ctgaataacg
     9661 atctggccga tgtggattgc gaaaactggg aagaagacac tccatttaaa gatccgcgcg
     9721 agctgtatga ttttttaaag acggaaaagc ccgaagagga acttgtcttt tcccacggcg
     9781 acctgggaga cagcaacatc tttgtgaaag atggcaaagt aagtggcttt attgatcttg
     9841 ggagaagcgg cagggcggac aagtggtatg acattgcctt ctgcgtccgg tcgatcaggg
     9901 aggatatcgg ggaagaacag tatgtcgagc tattttttga cttactgggg atcaagcctg
     9961 attgggagaa aataaaatat tatattttac tggatgaatt gttttagtac ctagatttag
    10021 atgtctaaaa agctttaact acaagctttt tagacatcta atcttttctg aagtacatcc
    10081 gcaactgtcc atactctgat gttttatatc ttttctaaaa gttcgctaga tagagttcta
    10141 tattagagat gtaaagagtt ttggtgaaga ggtttgtgag tatggctttt ataaccaggg
    10201 tgcaatgaga agcactaaca gcagctagct taagaacgca taggatatac tttttatgga
    10261 agtcacagag gaggaataaa atttgaacag gattaacaga gtaacctcta ttcttattca
    10321 gctgcaatca aaaaaaataa ttcctgccaa agaaattgca cagcgattta atataagctt
    10381 aaggacagtt tacagagata tccggacact tgaagaagcc ggaataccaa ttggatctga
    10441 ggccggaaaa ggatattttc ttgtagaggg cttcctactt ccgccggtaa tgtttacagc
    10501 ggcggaagtg ggtgcattga ttacagcagg gaaattttta aattgccatg gagatgaatc
    10561 atttataaag gattttgatt cagctatgta taaaatcaaa tctattttga agcatggtga
    10621 aaaaaactat gcacaggagt tggagaacag tattaacgtg tacagcacat ctggacagaa
    10681 aaatacattg gcggataacg ttattgcagc aattcagact gcaatctgca ataaaagggt
    10741 aatatcaatt caatatcctg catcaggagg gcaagaaccg gaaagcagaa tgatagagcc
    10801 tatatcactg gggttttatg aacagaactg gtacttaatc ggttttgcag gctaagaaat
    10861 gaatatagga attttcgtgt ggatcggatt aaaagcattt gcatagaaga agagctctgt
    10921 caaagccatg atggctcttt ggaacaaata ttaaaacaaa tgctttcata taaaaaacta
    10981 tatgccctac aatgccgttg taaaaattgg ggaaaaggga tatattgatt ttatatcttt
    11041 ctcaaaataa atataaaagc cctttaaaga gggcttttat atattaatca caaatcactt
    11101 atcacaaatc acaagtgatt aatcacaaat cacttgtgat ttgtgattct taatgataca
    11161 atattactat acaaaaaaag aatggggcgt agttatggag aaggaaaaac taaaaatact
    11221 tgaagaatta agacgtattt taaacaataa aaatgaagca attattatct tgaataatta
    11281 ctttaaaggt ggtgttggaa agtccaaatt atcgactatg tttgcttact tgacagacaa
    11341 attgaattta aaagttttaa tgatcgataa ggacttacag gcaacattga caaaagactt
    11401 agcaaaaact tttgaggtag aattgccacg tgtcaatttt tatgaaggct tgaaaaatgg
    11461 aaacttggct tcttctatta ttcatttgac tgataattta gacttgatcc ctggcacgtt
    11521 tgatttgatg ttactgccaa aattaactcg ctcatggacg tttgaaaatg aaagtagatt
    11581 gcttgctact cttttagcac ctttaaaaag tgactatgat cttattatta ttgatactgt
    11641 accaacgcca agcgtttata caaataatgc aatcgtggcg agtgattacg ttatgatccc
    11701 tttacaagca gaagaagaaa gtacaaacaa cattcaaaac tatatttcct atttgattga
    11761 tttgcaagaa cagtttaacc ctggactaga tatgattggt tttgttcctt atttagttga
    11821 tacggacagc gcaacgataa aatcaaacct ggaagaactg tacaaagaac ataaagagga
    11881 taatttggtt ttccgaaata ttatcaagcg aagtaataaa gtaagtactt ggtctaaaaa
    11941 tggcattaca gaacacaaag gctatgacaa aaaagttttg tctatgtatg agaacgtatt
    12001 ttttgaaatg attgagcgaa tcattcaatt agaaaatgaa aaagaataga atcacaaatc
    12061 acaagtgatt aatcacaaat cacttgtgat ttgtgatagg tgatgataaa ataaatagta
    12121 agaagaaata gaaagaagtg agcgatcgtg ggaaatttag gcgcacagaa agcaaaacga
    12181 aatgatacgc caatcagcgc aaaaaaagat ataatggggg ataagacggt tcgtgttcgt
    12241 gccgacttgc accatatcat aaaaatcgaa acagcaaaga atggcggaaa cgtaaaagaa
    12301 gttatggatc aagccttaga agaatatata cggaaatatt tacctgacaa actttaaaag
    12361 gagtgaaaat gaaatggcag ttacgtatga aaaaacattt gaaattgaaa tcattaatga
    12421 gttatcggca agtgtttata atcgggtatt aaattatgtt ttgaatcatg aactagatac
    12481 taaaaatact cgtttactag aagtgaatct tttaaatcaa ttagaagtgg cacaagaagt
    12541 tgatttattt caacaaccat ttgaagaatt acaagctatt catgagtatt ggcggtcaat
    12601 gaatcaatat tcaaaacaaa ttttgaataa agagaaagtg gcttaacatg gcgaatatta
    12661 ctgacttcac cgaaaagcaa tttgaagatc gtttagaaaa gaatgttgaa cgactaacta
    12721 aaaatagact agcggttgaa tcgccaaccg cttttttact tggtgggcaa ccagggtcag
    12781 ggaaaaccag tttgcgatca gcaatttttg aagaaacaca agggaatgtt attgtcattg
    12841 ataatgatac ctttaaacaa cagcacccaa attttgacga attagtgaaa ctctatgaaa
    12901 aagacgtagt aaaacacgtt accccttatt cgaatcgcat gacagaagca cttattagcc
    12961 gtttaagcga tcaaggctat aatttagtga ttgaaggaac aggacgaaca acagacgttc
    13021 ccattcaaac agccacaatg cttcaatcta aaggttatga aacaaaaata tacgtcatgg
    13081 cagtaccgaa aattgaatca tacttaggaa cgattgaacg atatgaaacc atgtatgcag
    13141 atgatccaat gacagctagg gcaacaccaa aacaagcgca tgatattgtt gtcaaaaact
    13201 taccgaccaa tttagaaacc cttcataaaa cgggcttatt tagcgatata aggctttaca
    13261 atagagaagg agtgaaactc tattcaagct tagaaacgcc ttccattagt ccgaaagaga
    13321 ccttagaaag agaattaaat cgtaaagtat cagggaaaga aattcaaccg actttggaga
    13381 gaatagagca aaaaatggtt caaaatcaac accaagaaac ccctgaattt aaagcaattc
    13441 aacaaaaaat ggaaagcttg cagctaccta caccaccaat acccaaaaca cctaaacttc
    13501 caggtattta aaccttaaaa aaggaaaaga gtagttacca aaaaacggta actactcttt
    13561 ttttataaaa acatttcctc atgttggggt cccgagcgcc tacgaggaat ttgtatcgat
    13621 aagaaataga tttaaaaatt tcgctgtttt tttctaattt cattttgagg tgaattaatt
    13681 ggtagttatt agaggtgccc tatacctaat aatttatcta cattcccttt agtaacgtgt
    13741 aactttccaa atttacaaaa gcgactcata gaattatttc ctcccgttaa ataatagata
    13801 actattaaaa atagacaata cttgctcata agtaacggta cttaaattgt ttactttggc
    13861 gtgtttcatt gcttgatgaa actgattttt agtaaacagt tgacgatatt ctcgattgac
    13921 ccattttgaa acaaagtacg tatatagctt ccaatattta tctggaacat ctgtggtatg
    13981 gcgggtaagt tttattaaga cactgtttac ttttggttta ggatgaaagc attccgctgg
    14041 cagcttaagc aattgctgaa tcgagacttg agtgtgcaag agcaacccta gtgttcggtg
    14101 aatatccaag gtacgcttgt agaatccttc ttcaacaatc agatagatgt cagacgcatg
    14161 gctttcaaaa accacttttt taataatttg tgtgcttaaa tggtaaggaa tattcccaac
    14221 aattttatac ctctgtttgt tagggaattg aaactgtaga atatcttggt gaattaaagt
    14281 gacacgagtg ttcagtttta atttttctga cgataagttg aatagatgac tgtctaattc
    14341 aatagacgtt acctgtttac ttattttagc cagtttcgtc gttaaatgcc ctttacctgt
    14401 tccaatttcg taaacggtat cggtttcttt taaattcaat tgttttatta tttggttgag
    14461 tactttttca ctcgttaaaa agttttgaga atattttata tttttgttca tgtaatcact
    14521 cctgaagtga ttacatctat aaataaatac agaagttaaa cgatttgttt gtaattttag
    14581 ttatctgttt aaaaagtcat aagattagtc actggtagga attaatctaa cgtatttatt
    14641 tatctgcgta atcactgttt ttagtctgtt tcaaaacggt agatgtttta tctacattac
    14701 gcatttggaa taccaacatg acgaatccct ccttcttaat tacaaatttt tagcatctaa
    14761 tttaacttca attcctatta tacaaaatt
//
LOCUS       DAYYGO010000069        14595 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000069 DAYYGO010000000
VERSION     DAYYGO010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.071"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(144..710)
                     /locus_tag="ACHA5T_001826"
     CDS             complement(144..710)
                     /locus_tag="ACHA5T_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8887392.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(710..1666)
                     /locus_tag="ACHA5T_001827"
     CDS             complement(710..1666)
                     /locus_tag="ACHA5T_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFM8887393.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1666..2322)
                     /locus_tag="ACHA5T_001828"
     CDS             complement(1666..2322)
                     /locus_tag="ACHA5T_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFM8887394.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            2692..4710
                     /locus_tag="ACHA5T_001829"
     CDS             2692..4710
                     /locus_tag="ACHA5T_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8887395.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    4780..4986
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5137..7551
                     /gene="leuS"
                     /locus_tag="ACHA5T_001830"
     CDS             5137..7551
                     /gene="leuS"
                     /locus_tag="ACHA5T_001830"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFM8887396.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            7899..9452
                     /locus_tag="ACHA5T_001831"
     CDS             7899..9452
                     /locus_tag="ACHA5T_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFM8887397.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(9833..11617)
                     /locus_tag="ACHA5T_001832"
     CDS             complement(9833..11617)
                     /locus_tag="ACHA5T_001832"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8887398.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(11632..13353)
                     /locus_tag="ACHA5T_001833"
     CDS             complement(11632..13353)
                     /locus_tag="ACHA5T_001833"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8887399.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13453..14214
                     /locus_tag="ACHA5T_001834"
     CDS             13453..14214
                     /locus_tag="ACHA5T_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8887400.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4734 a         2695 c         2909 g         4257 t
ORIGIN      
        1 tcatgttttt gatttggttg gacttattac cgcagatata agcttttgag caccgtgaat
       61 aaagaggtta tgaaatcaac gctctaacct gagtattaga gaaaaaaacg caaaacatat
      121 catttatgtt ttgcgttttt tatttatctt tttttctcaa tacaatataa aataggcggc
      181 tggttctttt ggttgataaa ccgataattc agcacattat atgtttcctg tggcagtttg
      241 gaacaaaaag aatcaaccat atccagttct ttttctccac cttcatgacc atagtagacg
      301 acaaggatca atcgtccgcg aggcacaagt cgtttcaata tttcttccat agctgtgcgt
      361 gtagtctctg gcatagtgat gattgcttta tcactttttg ggagataccc tagattgaaa
      421 atccctgctt tgagattttg ttgttcagct atcacttctc ctaacgtttc gtggccttgt
      481 agaaacaatg agacacgttc ttctaactga cgctgttcta gcctttcacg agtagcatcg
      541 attgcttgtt gctggatatc aaaactatag acatgaccag tttttccgat gtgttctgct
      601 agaaaaaccg tatcgtggcc attgcccatc gttgcatcta cgacgtgatc ccctggttgc
      661 aagatttctt gcagtaatgt atggctgaaa tgcaaagcag tttgcaacat cagacgctca
      721 cctttttccg tacatcatat tttccttgga agctatctct gcgaagaagt tcttcatcaa
      781 ttgcattcaa gacttcccat tttttcaagc tccacattgg tccaatcaag gaatcccacg
      841 gcgcatcccc tgtcaaacga tggatgatga tctcttttgg tatcatttcc agctggtcgc
      901 aaatgacatt gacatagtca ggacggctca ttaattgaag gcgaccttca tgatagtccc
      961 gcaacattct cgttttgcgc atcagatgca tcaaatggag cttgattcct tggatatctg
     1021 aatctaaaat ggtgcgtctg acattttctt tcatcatttc taaactttcg cctggtaatc
     1081 cgttgatcaa atgcgtacaa acacggatat tatgtttgcg taatttagcc acgccatcta
     1141 aataagtttg ataatcatgt gctcggttga tcagttttga agtttcttca aaagttgttt
     1201 gcaatccaag ttctacccat aaatacatcc gctcgttcag ttcagcaagg taatcaacta
     1261 cttcatctgg cagacaatcc ggtcgtgtac cgactgataa tcctacgaca cctggtaaat
     1321 tgactacttg ctcaaatcgt tctttgatta tttcaactgg cgcatgggtg tttgtaaaat
     1381 tttggaaata aacgatgtat tgttggacat gaggccattt ttgatgcatc atgtcgattt
     1441 ctttctggaa ttggattgga agagggtctt caggtgcaac gatcatgtct cccgaaccag
     1501 atacactgca aaaagtacaa ccgccatggg caacagtccc atctcgattc ggacagtcaa
     1561 atcctccatc gatcggcacc ttgaaaattt tcccgccaaa ctcatttcgt aaagcgtaat
     1621 tccaagaatg atatctttta tttccttctg aataaggaaa agtcattaaa agtccttctt
     1681 tctgtaagag ttatctattt gttttctttt ttctctatac actggataag tcaggcacag
     1741 ccaagaaaga ctgagtaagt agccgccgat gatatcagta ggaaaatgaa cgcccagata
     1801 aatgcggctt atcccgacac ttactataat aaacatcatc agcaattgaa gcaatgtgcg
     1861 taaggttctt ttttgaacaa aagctggaag cagaaatagt aaagaaccgt aaagaatcat
     1921 actgcctgct gcatgtccac tagggaaact gtaacttgtt tcaactacta agtgcgccaa
     1981 tgaaggacgc tctcgcatga aaaatagttt gatcaccggg ttgatgattc cagcaattac
     2041 aataaatcca gaaacgaacc aaaatgtttc tgcccttttt ttcttatacc acatccataa
     2101 agccgcacaa gcaaataaaa tgatgacggt aagaggatta ccgaatttag tcataaagag
     2161 ttggtagctg ttcagatcag gataagaagc tctgacaatg cctgtaaacc actgatcgaa
     2221 accagaaagc cacgacagat ggaactttac gatccagccc aaaatgaaaa acaggacaag
     2281 aaagaagcta ccagccacct gaaatgatgt tttatatttc atacaattcc tcctttgaag
     2341 caatgactta ttatagcatt tttggtccag cgaaaaaaac ggtaaagaga taaaaccaat
     2401 gtatgattcc tctttgaata accaatattc ttataaaaaa aataactgta atcggaaaga
     2461 cattttttgg taatgcaaat gaaatgttag ggaaataaaa tcagtgtttt tcgaaatcct
     2521 tttaaacaag aacgtagacg taggaagaac ttgaaaatga acagaagttg ttcattacgg
     2581 taaattacaa tatgattaca aaaaacaaca aagctcttaa ataactttta ataaatatta
     2641 agagaagtta taattattaa taatagataa attgaagttg gaggatagaa gatggaaaac
     2701 attgcaagaa aagaacggag acgtttagaa cagaccaaac gttttcgaaa agcaaaacga
     2761 ggggcagcaa tcgtgggaac tgcgatggta ggctgttctg tcgctgcccc attagtacag
     2821 ccggttcctg ttcaagcgga tgaaacacct gttcagttcg gcgcaagaat caatacagcg
     2881 gcatttatca cagaaattgc tacttatgct caaccaatcg ctcaagccaa cgatctatat
     2941 gcatcagtga tgatcgcaca agcagttgta gaaagcggtt ggggcagtag tgcgttatct
     3001 caagcacctt attataatct ttttgggata aaaggcagct atcaaggaca aaccgtctac
     3061 atggatacgc ttgaattttt aaataatcaa tgggtaaata aaaaggagcc tttccgacaa
     3121 tatccttctt ttgctgaatc tttcaatgac aatgcctatg tacttagaaa tacttcattt
     3181 ggcaacggtt attactatgc cggtacttgg aagagcaaca cgaaatccta tacagatgcg
     3241 acagcttgtt tgacagggcg ttatgcgact gatcctggtt acgcaggcaa attaaataat
     3301 atcatcgcaa cttacggatt gacccaatat gatacaccag ctagcggaaa cgcaggcggt
     3361 ggagcgacta caggtggcaa taatactgga agtgcaggta gtactggcgg ttcaagcaat
     3421 tctggtaata caaactcagg ttcagcaact tcgaccactt atactgtcca atctggtgat
     3481 tctgtttggg gaatctcaaa taaattcggt atcacaatgg atcaattgat ccaatggaac
     3541 aacatccaaa acaatttcat ttatccagga caaaaactga caatcaaagg tagccaggaa
     3601 aatggctctt cgacgaataa ttccggtaac aataccaact cttcaggaaa tgctggtaca
     3661 tcaaacggag gacaaacaac tggtgcgaaa tacactgttc aatctggtga ttctgtttgg
     3721 aagatctcaa atgatcacgg aattacgatg gctcaattga ttgaatggaa caatatcaag
     3781 aacaatttcg tttatccagg acaacagcta atcgtatcaa atggcggttc ttctaatgct
     3841 ggagcaaata ctggctcaaa tactgggaat acaagtggct ctagttcgtc cagctcaagt
     3901 tcttctaata cagcttcagg aacgaaatat acagttaaag ccggagactc tgtttggagc
     3961 gtctcaaata aatacggaat tacgatggct cagctaatcc aatggaacaa tatccaaaat
     4021 aactttatct acccaggaca acaactgact gtttcaaaca gtggatcaac aaacagcacg
     4081 tcaggtaata caggttcgtc aaccaataca aataacagca gttcagttaa tactggcagc
     4141 agttcgtctc aaggcactta taccgtgaaa gcaggtgaat ctgtttggag cgtggcaaat
     4201 aaaaacggca tcacgatgga tcagttaatc gaatggaata atatcaaaaa caacttcatt
     4261 tatccaggac agcaattgat cgttaaaggt ggaacttcgg caaatacaaa tacaggttca
     4321 actacttctg cagcgaaacc aaatacaccg aatacgtctg ctacgacaag tacatcatcc
     4381 actggtaata cgatgtatac cgtgaaagcg ggcgagtcag tttggagcgt agcgaacaaa
     4441 catcatatca cgatggatca gttaatcgaa tggaataata tcaaaaataa tttcatctat
     4501 ccaggacagc aagtgattgt gaagaaaggt tcatctcaat caacaaacca acaatcagtt
     4561 actggttcga aaacctatac tgtgaaatct ggagaatctg tttggagtgt tgctgacagt
     4621 catggaatta caatggctca actaatcgaa tggaacaata ttaagaacaa ctttatttat
     4681 ccaggtcaaa cgttgattgt agctaaataa aatagctgtt cgaagaaata acgattgaat
     4741 tccacaaaaa tatttgttac tataaggcta cttaaatatg acaacacttg aaaaggaagt
     4801 agtaaaaaga ctgaagtttc agagagcgca tggctggtga gaatgtgcac ggatgctttt
     4861 gaactcgcct ttgagttgcc cttgctgaat acccgagtaa gcaagcggcg gtgacggccg
     4921 ttacaacgtt cgaggccttt tcactaaggt aaaattaggt ggtaccgcgt agattacgtc
     4981 ctataaaaag agcaatcttt ttataggatt tttttatttc ttttgtgaag tttgagaaaa
     5041 caaagcaaaa ggaaataaaa ataaacagat tctgccggtt ttttcgttat atttataata
     5101 gaaacaagtg ttttcaatag aacaggagga cagcaaatga gttacaatca taaagaaatt
     5161 gagaaaaaat ggcaaaaata ctgggcaaaa acgaatactt tcaacaccca tgatgatcct
     5221 gaaaagccaa aattttacgc actggatatg tttccctatc catccggtca agggctacat
     5281 gtagggcatc ctgaaggtta tacggcaacg gatatccttt cacgtgtgaa aagaagtcaa
     5341 ggatacaacg tgctgcatcc aatggggtgg gatgcattcg gtctgcctgc tgaacaatat
     5401 gcgctagata caggaaacga tccagctgag tttacccaaa aaaatatcga aacattccga
     5461 cgccaaatca attctttagg atttagttat gactggaatc gcgaagtgaa tacaactgat
     5521 ccggagtatt ataaatggac acaatggatc tttacgaagc tttatgaaaa aggtctagct
     5581 tatgaagcag aagtagcggt aaactgggta cctgaattag gaacagttat ttcaaatgaa
     5641 gaagtgatcg atgggaaaag tgaacgtggt ggctatgatg tagtcagaaa accaatgcgc
     5701 caatggatgc tgaaaatcac tgcttatgct gatcgcttat tggatgatct agaattagtt
     5761 gattggccag aaagcattaa agaaatgcaa agaaattgga tcggtcgttc agtaggagca
     5821 aatattgaat ttaaagtggc tggtacggat aaatcctata ccgtatttac gacacgacca
     5881 gatactctat tcggtgcaac ttactcggtt cttgcacctg aactagatct tgttagagaa
     5941 attacgaccc ctgaacaaaa agctgctgta gaagcttata tcgaagagac agctaaaaaa
     6001 tcagatctaa agagaacgga tcttgcaaaa gaaaaaacag gagtatttac tggtgcttat
     6061 gcaatcaatc cagttaatgg caaggaaatc ccaatctgga tcgccgatta tgtattatct
     6121 tcatacggca caggagccat catggctgtt ccagcacatg atgaacgtga ctttgaattc
     6181 gctcaaactt tcggactaga aattcttcca gttatcgaag gtggagatgt tgaaaaagct
     6241 gcttacacag aagatggtac gcacatcaac tctgaatttc tagacggcat gaacaaacaa
     6301 gaagcaatcg ataaaatgaa tgaatggcta gaagaaaatg gcgtagggaa aaaagaagtc
     6361 agctatcgct tacgtgactg gctattctcc cgtcaacgtt actggggtga accaatccca
     6421 atcatccatt gggaggacgg aacagtcaca gctgtcccag aagaagagct accattacgg
     6481 ttgcctaaga caaaaaacat caagccaagc ggtactggag aatctccatt agcaaatatc
     6541 gaggagtggg tcaatgtcgt tgatcctgta acaggtaaaa aaggccgtcg cgaaacgaat
     6601 acaatgccac aatgggcagg aagctcatgg tattacctgc gttatatcga ccctcataac
     6661 aaaaaagaac tagcagatta tgaaaagttg aagcgttggc taccagttga catctacatt
     6721 ggtggagcag aacatgcggt acttcattta ctatatgcac gattctggca taaattctta
     6781 tacgatatcg gtgttgttcc tacaaaagaa ccattccaaa aactatacaa tcaaggaatg
     6841 atcttaggcg aaaacaacga aaaaatgtca aaatctcgtg gtaatgtagt caatcctgat
     6901 gatgtggtcg aaacttacgg tgcagataca ctgcgcatgt acgaaatgtt catggggcca
     6961 ttagatgctt cgattgcttg gagcgaaaat ggcttagaag gaagccgtaa attcttggat
     7021 cgtgtatggc gtttgatcgt tgatgagaac aataaaatgc gtgaccgtat cactacattg
     7081 aatgatggca agcttgataa agtgtatcat caaacagtga aaaaagtgac agaagactat
     7141 gaaaatcttc atttcaatac agcaatctcc caattgatgg tctttatcaa tgaagcctat
     7201 aaagtggacg ctctgcctta tgaatatatc gaaggatttg ttcaactact cgcaccgatt
     7261 gctcctcaca tgggtgaaga actatgggca atccttggaa atgatggtgg tatttcttac
     7321 gcaccatggc caacatacga tgaagcagca cttgttgaag acgaagtaga agttgttttc
     7381 caagtaaacg gtaaagttcg tgcgaaaagc aatgttcccc gagacttagg aaaagatgag
     7441 ctagaaaaag cagcattagc caatgaaata gtgcaagaat atattgaagg caaaacagtc
     7501 agaaaagtta ttgcggtacc aaacaagtta gtcaatatcg ttgctaatta attggctctt
     7561 tgtcaataag gactgatgag ttgtgcaaaa tcaaatctgg gccagaatga acttcattct
     7621 gtctcagatt ttttgttatt tcaaattgat taattgattg atcaaaaagg ttttgatttt
     7681 catgttctca aacacgttca atgtcttttt cttctgaatg gtcttgtaag agatctctgt
     7741 aatcatcaaa tttttgtcta ttaatcttag tattttttga tgtataaact acctgaaatt
     7801 gagagtacat accaaattca taaatttttt attagtggga ctagtgattg tatggatttt
     7861 gacgcataat gaatgaagta tgagaaaagg agagacaaat ggatcaagaa ctatttatta
     7921 tcgaagtatt atcagctaaa caaaaaatta tcgagaaagc gaaaaaaaga gcgaaaagaa
     7981 taatgttcaa gcttttaagc ttagtatttt tctctggttt gctttttttc ggagctgtat
     8041 cgttcctata tcgccatatg agtgcagtag tccgttcagc tgaagggatg caaaaattaa
     8101 ataatttgta tcacttttcg tggggagttg gcggaataac aattgttgtg cttattgttt
     8161 ttcttctact tatttggctc cgttaccatc aattgtcgcg tcatttcgat caatggctta
     8221 gtcaaagagc acaacagttt ttacataata aaccaacttc tcaagatgat gactatcttt
     8281 attttgagaa gacttcgacg agtcaagctt ttcggataca aaaaagtgat tgccagttat
     8341 tgctgagatc acttgatgat ttacctatct acttaggcga aagcaaatca aaattcggaa
     8401 tgaacacatt ccatattttt ttactagaaa acgaacccag atgggaaaaa agccagtctt
     8461 cgcctggtgg atacctgaca actaagatga gtacattagt cactggtttt ttcctcattc
     8521 ttttagctgg aacaatctat tatgggagat acactcagcc aaaaatgtct tctcatataa
     8581 atgctgcttc tcacatgaca atgaactcaa ctaacgatac gcatatacaa aagacaaatg
     8641 aagggttgtt agctgatcct aagcaaaagg taaatttgac aacacaaaag ataaatgatc
     8701 tcaaacttga tacagagacg cactctctct atatgacaac agatagtggt gctaattggg
     8761 tatttgtgcc gattgattcg caatggttgc gttttggcga atacaccctg acaacggggc
     8821 tgattcctga aggttattgg atggacaaca catatgatat ttccgccgat ttctcatggt
     8881 ttatctattc ttctggggat gatgtacgtc tgctgacttc aagagataat ggtaaaacat
     8941 ggcaaacgtc tattatcacc caatcagttc agccgatacg ttatcgaaaa gcggtatttt
     9001 atggcaacga tggtctattt atctgtacat cagccagtgg gatgtctgca gaaactttgt
     9061 acatttacac tactatggat ggcggaatca cttggataaa aagtggaagt acaatgatca
     9121 atcagccagt acaaaatgtc agcttcgtta caacttcttt aggttttgtt tcgacacgtg
     9181 aaagtttgtt ttatacaaac aacggaggac gctcattcaa agaatcagtt ctgtcgattc
     9241 cggaaaacta tcagacaggg gggatagatc tttttcaatc accaaatgaa gttactcaaa
     9301 taagcgcgaa tacgttagag acaaaatttt atttgttgaa aaaaggacca atcgatgcag
     9361 ggaaaatgtt tgcctgcctc ttccgttcaa ctgataacgg ggaaacatgg caattcgttc
     9421 agcaactatc gcaagtagaa caaggagatt aaagaagaga gattgtgagc ctgacgttca
     9481 gcaccgagtc acaagaaaca aattcaaaaa acagcttctc atatttgttg aatagtgtaa
     9541 ctcgtgattc ggagttgaca agagcaacaa gaagcgattt tgaagtatag cttctcgtat
     9601 tttacaagat cgtgtcttat tgccgatagg ttgtctcttg aacaccgttt attcgtaaaa
     9661 agggattgtg acaagtcttt tgtcactact taccgaacga aactgaagga gctatccaaa
     9721 aagtatcatt agtattattc tttgtgtttc tagtggcacc actagtacat ttaaaaaagc
     9781 tatcgatcat ggactaatca tacatgatcg atagcttttt agataatgtt tgttacgcta
     9841 tctgaaattg actattataa agactggcat aaagcgttgg tgattggagt agttcagaat
     9901 gtgttccttt ttcgacgata tcgccattct tcattacaag gatcatgtct gcattttcaa
     9961 tcgttgacaa acggtgtgca atcacaaaac ttgtcctatc ttttgtcaca gcttccattg
    10021 cttgctgaat caatgcttct gttcgtgtat ctacgctaga agttgcttca tctaaaataa
    10081 cgacaggagg attcgctaga atgactcgag caattgtcaa taattgttgc tgtccttgag
    10141 ataacgtgcc gttttcgcta gaaatgatcg tatcatatcc gtctggcagt gttcgaataa
    10201 agtgatcaca ttgtgcgatt tcagccgctt cgataatttg ttctcttgtg gcatccattt
    10261 tcccgtaggc gatattatca gctaccgttc cttcaaacag ccaggtattt tgtaaaacca
    10321 tcccaaaaga acgacgtagt tctccacgtg aaagcttggt aatatcaaga ccgtcgaatt
    10381 cgatctttcc tttattcagt tcataaaaac gcatcagcag attgactaaa gtcgtttttc
    10441 ctgcaccagt tggaccaacg atcgctaccg tttgttttgg ctttactgaa aaatcgactt
    10501 gattcatcaa tattttatct gatgtgtaac caaactgtac atttttgaaa gctatttttc
    10561 ctttcggttg ttctaaaagt cttgcatctg gaaggtctgg aatttcatct gtttcatcta
    10621 atatctcaaa tatcctatcg attgctgcca ttgcggattg gatcgagttg atcacataag
    10681 aagcggtaga aattggttca gaaatctgat tgatgtactg aagataagct tgtaagaagc
    10741 cgatcgtcac accacctgat agaacaagca tagcaccgaa taccgcactt atgatgaagg
    10801 cgagttgatt aatgaaacga atggccggat agatagcaca gttcataaat tgagcacgtt
    10861 taaaagcgtt gtattgcttt ttatttactt cgtgtatagc agcttctgca tgagcttgct
    10921 gattgaatgt tttgaccact agattaccag ctaagatttc ttccattcga ttatttaaaa
    10981 cacccagttc atactgattc tgttcagaaa tctgtttgtt tttgttcgca atcttagttg
    11041 taacaattga actgccaacg atcagcaata agacgagcaa agtaagcttg ctttcgatat
    11101 aaaacagcat cagaccagca gcagctattg ttacgacaga agtaaaaaat tgattgatac
    11161 ctgtcaataa cacttgagaa agttgattga gaccggtagt tgtccgacta atgatgtcac
    11221 ctacttgatg attgtcaaaa aacgacatcg gtaatgtttt gaattttttt gtgatttctt
    11281 ttcgcaccct caatgtgatc ttttcgctta acgctgccat tgtccgttcc tgaataaatg
    11341 aagtgatgct gcttaagact gaacagccga tcagcaataa gacagggaac aacaatgctt
    11401 gtttgatatc actgaaagaa agctgtccaa tacctgtttt tcttatttct gataagagat
    11461 catcgatccc gattcccatg atcaagggca tggctacgac taataggttc ccgaataaac
    11521 tgcatagaaa caatccgtaa aaatatttat gttcaggttt gatcattttc caaaaacgta
    11581 tcaaagcacg cgtgttcaac gtttttttct tttccatcgt atcccttctt tctatattcc
    11641 ttgtgatttt gcaaactcct gataagtttg gcaattagac agtagttctt catgagtacc
    11701 ttgtccaacg atttttcctt catctaaaac aatgatgtta tctgcttgtt ggattgtact
    11761 gaggcgttga gcaacaatca cgagagtttt ctccgacatg tgtttatgaa gggctgctct
    11821 taatgctgca tctgttttat agtctaaagc ggagaaacta tcatcgaata catagatctc
    11881 tgctggtttg atcaacgctc gagcaataca tatccgctgt ttctggcccc ctgaataatt
    11941 agaaccgcct tgagcaacaa agctgtccaa tccgtcagga agggtgtcca ctagttcttc
    12001 taattgagcg atcttgatcg ctttagacaa ctcttctgtt gaggcatatt catttcccat
    12061 ctgcaagttg cttaaaattg tcccgctgaa taagaaagcc ttctgaggga cataactgat
    12121 gtgggagcgg atctgttctt gagtcatttg agtaagagga ataccaccaa gggttacttt
    12181 tcctatcgta gggtcactaa gtctcaagag aagtttcgcc aaactacttt ttccagatcc
    12241 ggtcccgcca acgatcgctg ttgtttttcc tttaggtatt gtgaaatcaa tatcttctag
    12301 aactggatca gagatttcag tatatttaaa tgtgacatgt tcaaaagcag ctgcatttgt
    12361 ctgatcaaag gttggttcag ctactgtttt gatcgtctct tcctcaattt cgatctctgc
    12421 ttgcagtatt tcttctatcc gtcgtaatga ggcaaacgat ttagggataa tgaccaatac
    12481 catagctgcg atcatcaagt aagcaagtgt cagcatagag tattcgacta cagcagtcac
    12541 gacaccgatt tgtaatgctc cttctgctac aagatttccg ccaaaccaca aaatagaagc
    12601 ataaacgatt cccatgagca aaaaagcaat tggagtgacc caagcaaata ttcggttcac
    12661 tttgatcatt tgattagcat agtcagcaaa gctcttattc gttcgctttt cttcatatga
    12721 ttgattatca aaggcacgga tcatattaat cccagtaaag aattctctca atgtgatggt
    12781 gatccgatcc atttttggct ggatactcaa agataaaggt gcccctcgtt tcatcagtcg
    12841 ataaacaatc catacataaa aaacaacaga gatcaatgta acccatgtta gtaatagaga
    12901 ataacgaaac atcatgagaa tggtgaaaac gcaaataacc ggtgctggta agatcatttg
    12961 gcaaaataaa acaatcattt gctggacatt atcgacatca ttcgtcattc ttgtcaaaag
    13021 agatccagaa ctaaaacgac tgatatcctt cattgacagc tgttggaatt tacggaaata
    13081 tttctcccgc gtttggaatc caaacctagc ggaagccaat gctgaaaagt aactagaggc
    13141 aactgcagcg attgttccta gaaacgctac aagcagcatt tcaactccaa ttttttttat
    13201 aactgaggct tcgccacttg ctatacctgt gtcgatcagc tttgctacta attttggtac
    13261 acctaatgta ccggcgattt ctaaacagat catcacaaag gtaaggagag cgagtccttt
    13321 gtttttctgt aagaatgttt ttaagataga catattattc ctcctttttt cctgatactt
    13381 ttcgattcta taccttcaag taacttgaag gtcaatagaa aaaagaataa actgaaaaag
    13441 aggtgagaaa aaatgaaaaa aatgtatacg attggccaag tatcggaact atttgatttg
    13501 ccaaaatcaa cgatccgcta ttgggatgaa caaggattaa tccgttcaag tagacaagaa
    13561 gagaatgatt atcgattgtt tgatatcgat gatattttta tgctttatga tatcgttttt
    13621 tatagaaaat tagatatacc gatcaaacag atgaagaatt tatatggaaa aacattgacg
    13681 gagttatatg agacacttga tgaaacagaa agaagaatcc ataaagaact ggtggtgatg
    13741 aaacaaaaac aaaaagagat aagggagcaa aaaaaacagt tgaaattgat gatcgataca
    13801 aacgaggaag aatttcctgt agaagagatc ccttttgact gtatgatctc aacggagttt
    13861 gaagatattg tggaaatcaa aaaatttcta cctaactatt caagctttgg gatgatgagt
    13921 atgccagctt ccagttcaca gacgatgtat ggatttttta tcgaccctag tgaagtacat
    13981 ttatttactc aagacgtgat ttgggagaaa aaagatacgg ctgtttatcg tcgattctta
    14041 ttgaaatccg agatgaacca tgcggaacgt aataatattt tagaaatcag agaaagaatg
    14101 tatgaaaaag gatggaaaac aggagaagtg atcggccagt atctattgac gaatacggat
    14161 gaaaacaata tcagaactga atattatcat gcatggatag aaatgaaaaa ataggaaata
    14221 tgtgtttaag ataactcgaa aatgatggtt gttttagtta taaagaaaaa ttttctggtt
    14281 cgattacggg tttaatgtgg gggattatta gtcgtggata gatagaattt tagtttttta
    14341 gaggaaatat gtctttaaaa atggaatggt tgcaattctt agcgatatat acgatgtaat
    14401 tgtcctctta aaaaagtatg gaaaaataat tgtattgggt gttatgtata attcatggtg
    14461 aactgagact aatgagaaga aaaattcata ttgttatgag tgagattgta tcagatgaga
    14521 atgataaatt aaataggtgc ataaaagcat tacacttaaa atacattgtt gcttacatta
    14581 ttacaatatg ttaat
//
LOCUS       DAYYGO010000070        13964 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000070 DAYYGO010000000
VERSION     DAYYGO010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13964
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.072"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..239)
                     /locus_tag="ACHA5T_001835"
     CDS             complement(<1..239)
                     /locus_tag="ACHA5T_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFM8887401.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQA"
     gene            complement(718..1437)
                     /locus_tag="ACHA5T_001836"
     CDS             complement(718..1437)
                     /locus_tag="ACHA5T_001836"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFM8887402.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(1486..3132)
                     /locus_tag="ACHA5T_001837"
     CDS             complement(1486..3132)
                     /locus_tag="ACHA5T_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFM8887403.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            3311..3901
                     /locus_tag="ACHA5T_001838"
     CDS             3311..3901
                     /locus_tag="ACHA5T_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8887404.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            3993..5498
                     /locus_tag="ACHA5T_001839"
     CDS             3993..5498
                     /locus_tag="ACHA5T_001839"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFM8887405.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(5582..6934)
                     /gene="celB"
                     /locus_tag="ACHA5T_001840"
     CDS             complement(5582..6934)
                     /gene="celB"
                     /locus_tag="ACHA5T_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFM8887406.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6934..7452)
                     /locus_tag="ACHA5T_001841"
     CDS             complement(6934..7452)
                     /locus_tag="ACHA5T_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887407.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(7468..7794)
                     /locus_tag="ACHA5T_001842"
     CDS             complement(7468..7794)
                     /locus_tag="ACHA5T_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFM8887408.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7807..9786)
                     /locus_tag="ACHA5T_001843"
     CDS             complement(7807..9786)
                     /locus_tag="ACHA5T_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8887409.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9807..10121)
                     /locus_tag="ACHA5T_001844"
     CDS             complement(9807..10121)
                     /locus_tag="ACHA5T_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFM8887410.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            10289..10582
                     /locus_tag="ACHA5T_001845"
     CDS             10289..10582
                     /locus_tag="ACHA5T_001845"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFM8887411.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10660..11772)
                     /locus_tag="ACHA5T_001846"
     CDS             complement(10660..11772)
                     /locus_tag="ACHA5T_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM8887412.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            11925..12773
                     /locus_tag="ACHA5T_001847"
                     /pseudo
     CDS             11925..12773
                     /locus_tag="ACHA5T_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(13126..>13964)
                     /locus_tag="ACHA5T_001848"
     CDS             complement(13126..>13964)
                     /locus_tag="ACHA5T_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM8887413.1"
                     /translation="YQLVETQAPTGYELDATPIEFEIERSQTAVVELTKANRLTPGGV
                     VLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAP
                     TGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDA
                     NGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIF
                     LTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKR
                     NI"
BASE COUNT         4359 a         2855 c         2390 g         4360 t
ORIGIN      
        1 gcttgatccg aagaaaataa aaatttgaaa ttttcccaac ccacccaagg actttccctc
       61 aagctagcca agaacccatc tgctgaaata tgaaaatttt tgaatgctac gacattcgcc
      121 attacaggta tgtaaaagaa aaaaatcatc caaagcatac ccggtacagc catcaggaca
      181 agtgctttat aacgccatag attcataaaa aaatgattga acccctttcg ttttttcatc
      241 taaaaacccc tttcgattca ttcgcaacgt aattatagcg ctttcaataa tgagaataaa
      301 taaacaaagt atagggaaaa tgaaacaaag tataggagag gttgtgaaat cagcgttttg
      361 gcctgagtat tagagaaaaa aacgggaaaa tagttcctca tattttgccg ttttttgagc
      421 taataccgag ggtcagctga ttgaacaccg tttatcagag gttgtgaatt cagcgttttg
      481 gcctgagtat tagggagatc gtgtccttac tatctaactc tgagtcacaa ggaacaatgg
      541 ggactgtaac aaaagtcttg tcacagtccc ttattccgaa caaacggtgt tccagaagta
      601 gcttctacgg aaataagccg aaatttcaca aaaaagccat ccgaaagtca gacggacaag
      661 tctgcttttc agatggcgct acaactactc tgcttattct tttagctatc cattcaatta
      721 tttttctctc acttgttcta gctcttcttt ggtcatgggt cgatattgcc ctttattcaa
      781 atctttaggc agccaaaatt ttcccatacg gattcttttt aaatataaga cttcttttcc
      841 tactgctttg accattcgct taacttggtg aaactttcct tcatgcaaaa tcagacggat
      901 aattgaagtt tctgtctctt gatctacttg atcaatttgg agttttgccg gcaatgtctg
      961 ctcatcgcca tcaataatca gtccttctgc aaataaacgg atatcttctt ctgtcattat
     1021 accgctaacc tcagctagat attctttctc cacatgtttt ttaggtgata gtaactgatg
     1081 ggcaaacacc ccgtcattcg ttaaaatcaa gaggccctct gtgtctttat ctaatcggcc
     1141 gactggaaaa agatcttccc gataatcctg gtcttccaat aaatcgatta cggtttgatc
     1201 atgattatcc tgagtagcag aaatgactcc tgcaggttta tgcaaaatgt agtaaaaatc
     1261 tttttgatac gtaatgattt ctcctaaata agtaaccgca tcttcgaatt catttacttg
     1321 cgctttatcg cttttcacca cttgtccatt gacttcgatc gttccttttt tgattaatcc
     1381 tttgacttct ttgcggctgc caagtcccat atctgctaaa aacttatcta aacgcatgcg
     1441 tttcccccat ttcaaaagag catcaatcta gctcttctga ttcacttatt ttatttttaa
     1501 tttatgtcgt aaacgcgcta ttcctggtcc tagtagtctt tctgccaagc ggattttcaa
     1561 agctaaataa ccatacacta aagcccctat acctgctact acaagaatca acaatagaga
     1621 ctggaatttg ctatcttcgt ttaaaaaagc accaaaaatc atctttgtga tccacgaagc
     1681 caaaagcata atcaaggtaa gaataaagat cagcaatgtc ctgcgccaga caaaacttgg
     1741 tttaaagcgg gctacttcat ggattctctt taagatcaag gtacaagaca cagcaaatcc
     1801 gatcattgtc gccaataaag ggccgtatac ttcaaataaa cgaatggctg ggtattgaag
     1861 aaggagtttc actagaaacc caataaccaa ataattaatt gccgccttgt tttcaaacat
     1921 tccttgcagc atgttggaaa ctagcatata tagtcctagg aagagtccta caaaacttgc
     1981 ttggatcagt acattacttc ctagagaatc cggtgtataa aataatgtat ttagcggata
     2041 ggcaagtaac ataacaccaa atgtcgccgg aaacataaaa aatgaaaaca actgtaaatt
     2101 tccgcttgtc aattttgcca gacctactcg atctttcaac gtaacagctt ctgtaatcaa
     2161 cggtaaccca gtagacgcga tcgatgttgc caatgcaata actaccatcg tcaacttatc
     2221 tggattggca ctaaaaatcg aaaaaagatc cagtaactgt gcattggaat actcggtgga
     2281 tgcagacatg atcttcataa aagtgaactg atcgactaat ttgaagatcg ttacccctga
     2341 acccacaata ataaacggaa tcgcttcttt aacggtatct aacaacaatt ccttcgttgc
     2401 aatatttatt ttgttttcgc tgtagtgaat aaacgccgat gtatacgctt gatgtttctt
     2461 caaaaaatac aaaaggacaa gaatacttgc tagcataccg ataaatgccg cgaaagttga
     2521 ttgcgtgacc gccgtcacat agtcaccatc catgactttc atgatgatga atgtagccaa
     2581 tagcatataa aaaatacgtg ccacctgttc tacaatctgc gacagcgcat aaggcatcat
     2641 ttcttgattg ccttgcaaat accctcgtat cacactcata caaggaaaaa ccaatacagc
     2701 gatactcaaa gaacgcatcg ttggaatcag ttcttctccg ccaccagaag catgagctag
     2761 ccaaggagag gcgatgtaca tgaacaaagc aaacaacagg cctaacccgc ccatcatctg
     2821 taatgcacgg ataaataatc gtcgtgaagt gccatattca ttcaatgaat tatagcgtgc
     2881 agtttgtttt gcaatcgcag caggtattcc tgctgtagag atcagtagaa acaatgcata
     2941 tatattgtat cccatattga ataatccatt ggcagcctta gcattttcgc ccatccaagc
     3001 ataccaggga atgatataga cagcgcctag caatcttgaa atgatattac tggcggtcat
     3061 ccaagcagag cctcgtgcca ttttttcttg cggtgttaat tgatctaatg ctggttttct
     3121 ttgattatcc atcggtcaga ttccaacttt ctacaaaaca ttactatcat tttaattggt
     3181 aaaacagaaa ctggcaatcg attttatata acttttataa gaacaattgt ttttttgaat
     3241 tcggtttcgt ttttttggta aagtaaagaa atatacagcc ttttatggct aacatttata
     3301 ggagatatca atgacacaag aaataaaaaa actacccgtt atatttttag gtcttttctt
     3361 tatcacagtt ggattgaatt ggttcttgct ccctcacgat atagcttccg ccggagtggg
     3421 cgccatcgga catttagttg aaacggcctt ttctatccaa cgctcagtta ctgtttggag
     3481 catcaatctt atgatgctgc ttctagccgc tgtcctatta ggaaaatcag tattttttaa
     3541 gactgtagcg ggaagtctgc tttttcctgt tgttttagag attgttccaa aagtggaaat
     3601 gatctcctca catttctttt cactgatttc aggaagtctt ttgttcagtc ttggggttta
     3661 cacattgtac acagtcgggg cctcaaatgg cggtataacg ataccgccga ttattttcca
     3721 caaatttttt cggcttccaa tgccgcaagg acttttactt accaatagtc ttatcgtctt
     3781 tttgaattat tatgtttttg gattgttgga gacactattc gttctcctat ctattacagt
     3841 cagttcattg tttatgaaaa tactcatccg cttgtcccct gtgtcaagaa aatttgaata
     3901 aagctggatt ttgtcatagc ggaaacaaac gatatattaa aaagagtgtg ctataataaa
     3961 ccaagaaatt gaataaagcg agggatcaat caatgagctt ctctttagaa gacatccgtc
     4021 atttactatt aaaagaaaat ctattaaaag aatttgtttc aacaagaggc tggcatcttg
     4081 atgtaccaga aaaagctgaa tttcttcagt taagttatga ttcaagaaaa gctgatgctt
     4141 ctactttgtt tttctgtaaa ggaatgaatt tcaaagaaga atatttggat tcagcaatcg
     4201 aacagggtat tcaatattat gtatcagaac aaccatacga aaattcagca gtaggaatca
     4261 tcgtaacgga tatccgtaaa gcaatggcgc ttcttgctat ggctttttac gactatccgc
     4321 aaaataaatt gaaagtgatc ggattcactg gaacaaaagg taaaacaact gctgcgtact
     4381 ttacaaaagc aatcttggat cacacaacaa ataaaaaaac agcattgctt tctactatga
     4441 atacaacgtt agatggcaaa acatatttca aatcccattt gacaacgcct gagtcattag
     4501 atttatatcg tatgatgtca gaagcagttg aaaatggtat gactcatcta gtgatggaag
     4561 tttcttcaca agcttacaaa acacaacgcg tatacggctt gactttagat gtcggaatct
     4621 tcttgaatat ctcgcctgac catatcagtc cgatcgaaca tccgacattt gatgactatt
     4681 tctattgcaa gcgacaattg atcttgaatt caaaaacagt tgttttaaat catgaaagtg
     4741 actattttga tttacttagg gaaacagccg atctattcca gattccaacg atcacttatg
     4801 gtcgttcgga agaaagcaat tatcaagtga tccgttcaga taaaggtact cacggtttta
     4861 ctttatcttc tcatgaagat cgcttagcta tctgcgatac tgcttatgat attttgctgg
     4921 ctggaggatt caaccaagaa aatgcagcca gtgcgatcat tgccgcagca ctaacaggtg
     4981 cctccagtca agatgcacaa gacggattaa aagaagctcg tgtaccaggc cgaatggatc
     5041 aattgatcca atccaatgga gcgcatgtct atgtggacta tgcacataat tatctaagct
     5101 tgaaaacatt gctgaaattt gccaaaaatg aacatccaga tggccgtgta attgttgtat
     5161 taggcagccc aggaaacaaa gcaatctccc gtcgacatga ttttggaaaa gtgctatcag
     5221 aaacagctga catcgccttt ttgacggcag atgacccagc atttgaagat ccgaaaaaaa
     5281 tcgcggaaga aatcaatgaa gcaattacta atccagattt gatcgttcat tatgaaatgg
     5341 atcgtcctga agcgattcga cgcgctttag cagaaagcgc tccgcaagat tcagtcgtta
     5401 ttgcgggaaa aggcgtggat ccctatcaaa aaatcaatgg tgtagatgaa ccttatgaag
     5461 gggattatgc aatcgtgaag cggttgattg aagaataatg gtatttatat actagtggtt
     5521 tcccatttct aagaaaaaaa gggactgtga ttttttgtca cagttccttt tttcttagaa
     5581 atcattttaa cttttcacta gctaattctg ctgccttttc tctttcttca tttaataatt
     5641 tattatcata gaatttgata aatgggaacc agataatgaa tgcgatgatt gcacaaatga
     5701 ctgctaacac agcagctttc caatcaccac cactaccaac aaatgctccg atcccgactg
     5761 gtgaaggcca cggcatctgt gcaagcatcg gtttgacgat ttccagccga atggcaaaat
     5821 aagcgagtgt agcagaagcc atcggagcta agaaaaacgg aaccgctaaa tagggattgt
     5881 agacgattgg catcccgaaa atgattggtt cattgatatt gaataatcct ggtacaagtg
     5941 aggcttttcc taacactttc agctgatcag atttcgcgag gaatgcaata aagatgatca
     6001 atcctaaagt cgcacctgat ccaccaacgg ttacataaga attgttgaat tctcctgcca
     6061 acggataatt tgcgccttgt gcgttagctg ccatattagc taatacgatc ggggtcaaga
     6121 aagaagtgat gatattcgct ccatggatcc ctacgatcca caatgcatgg atcaagaaat
     6181 aaatcaccat gacaccaagc catgtattcg tcaactgcgt aacaaaccca aatgggatag
     6241 caatcatttt gaagatatcc gttcctagtg caactaataa tccattgatg atcatcacca
     6301 caaacgcaat gacaaatgtt gggatcagag cagtgaaaga acgagaaact cctggcggaa
     6361 caacatctgg catttttatg acccaatttc tcttgacaca catacaatac aattcgaccg
     6421 caataatcga cataataata gctgtaaaga ttcctgaagt tcctaaacgt tcgacaaaag
     6481 agcccatcct aataccattt actacagttt ctgtctcatt gatagaagca atcaagctga
     6541 ttttaccatc agtcatgatc aattcaggga tacacataaa gaatgcaaaa acagatagta
     6601 atgcccccgt gagcggatta acatttaatt tttgttcatc tcgttcaatt gacgttaatt
     6661 cataagcaaa cacaatggca aagtaaatag ataaaatccc catcgtcatt gtattgacga
     6721 tcatgtacaa gtcactaact ttgaaaaaag ttgcattaaa aaatccttct aaaaatggga
     6781 acggtaatgg caaaatattt aaaaccaaaa acatcgagcc tacaatcgtg aaaggaatag
     6841 aagccatccc cgctgccatg acagcccgaa cgattttaag ttgcgcaatt ttccccatag
     6901 gtcccatcag gtatttctgc aaaaaatcaa acattagtta ttctcctttc ctaaatataa
     6961 cgctttttca tattgtttaa tacggatata ctgttggtct tggttgagtg aaattttctt
     7021 taaacgatac tgtataaatg cactggcaaa aataccgctt acaacaagaa tgaaaacaaa
     7081 taactgtcct ccattgttat ttgccataaa aggatacaag aatgtaaatc ctgaggtaaa
     7141 caacactgga atcactaata aattaaagac actctggaac aaaaaatacc actttgtcca
     7201 tggaacgttc gtttggtgtt ttcgataaag ctttacttgt tctcctactg caggcaaaat
     7261 agctaaaatc agaatcatcg gtaaactact tcctaaactt ttagctgata gaagcagaat
     7321 catccattgc atgttcatga aaaagaaaaa ggcagtcagg taacgtacga ttagataacg
     7381 attgaaatac atgtttttca aactaagttg ctttttttct aattcgattt tcctttttac
     7441 tgtatcctgc acaatgatcc ttcctttcta tttatttttt tgatacaaat aattcatttc
     7501 ctgtgcaact tccaataatg tcatcgtcgt catcagatga tcttgtgcat ggaccatgat
     7561 aatttccatt tctatttttg tcccgctggc atattctttc agaagttgag tttgtgactg
     7621 atgcgcatga agaatctctt catttgcttg tcgcaacttc tcttctactt cttcaaacat
     7681 ctctttacgc atcgagcgaa atgcttcatg tatcagtgtc cgtgcatttc cgctatggaa
     7741 aatgatatca aaagaagtca cttgtagtgt ttcactgttt attttttcag ccatctgttt
     7801 tcctccttat tcgatcagtt tgataaattt actgctgaaa ttctcaaaaa taggttcagc
     7861 taagatttcc tgctgtactt ttagctggtc aactaaccta acgattgctt tagtcacgac
     7921 cgtaattcct tcattttcga cataagaagg cgaaactaaa aaaacaaaat gaatttcttg
     7981 atattgatcc cactgcatcc cagaaggaat caatgcaacg gctacctttg tttcgattcc
     8041 aactggaaca gcgggatgtg ggactgccac tgtatcactg aatatgattt gtcccatatt
     8101 ttcacgttgc tggatctgct ttcgcatttc tcttatatat ccttcttctt catttatgct
     8161 cagcttttgc agcaaatcat gaagaatttc ttcctttttc ggttcgtacg tataagtctt
     8221 gaaaaatttt tcagacaact gttcggtgta aatctgcttt ttttgctgta gagaaaatgc
     8281 tggtatcggt ctatctgagg aatgataggg tatagattct ccaactacct ttcgtatttt
     8341 acgaacatca tcttcattca aaaaaatact gacatgtaaa actggaattt tgaaaaacat
     8401 tgtcgaaaga tcaatagaag aaataatcag gtcgattcct tttaaagttg tttcattgat
     8461 ctcataatag ccttttacac ttaccactgt aatattttta ccgaattcac tgactacgcg
     8521 attctttaat agttgggcac tgccgtaacc cgttgcacag ataatcaatg catgtagttt
     8581 atgcgcagct ctttcttttt ccaatgccgc catgagatgc aaagccaaat aggcccattc
     8641 atcttcattg atggtatagt ttttcaaagc cggcatgttg ctgaaatgcc tttttactgc
     8701 gtcaaagact ttgggatttt ctttctttat ctcctcagtc aatgggttct caatgcgaat
     8761 acctcggctc aatcgaatca tcatcggtga caaatgatcg agtagtccat tgaaaagctg
     8821 gagatcctct actaatgaaa aatgaaattc ctgagacaat cttttcagaa ctttcgctaa
     8881 ttcctttgat agttcctgat cattttgatt ttctttgtga ttcgacttag ccatgagatg
     8941 taatgtcaaa taagcaattt cttcttcggg aaacgataca cctgcgatta cctcaatacg
     9001 ttgaacgatc cgctgcgcca cttgatactc tactcgctta gaaacttctt gttcgatgcc
     9061 taattccttg atttcaaacc cttctcggag ccgtttgatc cccaaagcca agtgtaaagt
     9121 cagattctgt atgataaaat ccgaaacttt taacttagct tctcttgttt catcgagaat
     9181 gataatgact agctcttcaa aactgatgtc tgggaaaaaa cggctttgcc ctaaatattc
     9241 tttcaatgaa tttccataat ggtttccaaa aaaagtatcc atgataaaat gccgtcgatt
     9301 tctttcttcc ccttcgatcc atgtcccttg accatgctta gaaacgattt ttagtgaata
     9361 tggaatcaat cgctccttga tttcttgcat gtcctttgtc aaactggaac gactgatata
     9421 aagctcttct gccaaatcat ccataaaaag aatggcatct tccaaaagca attggttcaa
     9481 tatatacttt tgtcgatctt cggactcata aaatccttgc ggttctctag catatccagg
     9541 tactatttct tgccgactca aaaacaagtc aaatgtcagt ctttgtaaaa tatgcagttg
     9601 gtagccataa cctggtttag caatgatctt acctccgttt ttttctacta actctttgat
     9661 ccgctgcaag taattacgga ttgtccgatc agataataac aattcagaag ctaaacgttg
     9721 actggtgaca tactccttct tgtgatcaat caaatatctc aacagcaatt tttccttctg
     9781 tttcattatc atccactcct attccatcat atgttctctt cgatcacctc tgctaacttc
     9841 tggatgcctg tagggattgg gacatacgct tggaaaggga tactaacaac aggctttcca
     9901 acagattctc ctaatttttt gaatttatcc aaaaacatcg tcgtttgcgg gctgatcaga
     9961 aataaatcaa aatcactatt cttaatcata ttatcgcctt ctgtagctga aactgcatct
    10021 acgagtacct cttctctttt ttgcttaaag aaatccgttg tctttgaggc catcatcgag
    10081 gatgacattc ctgccgcaca aataatcaat gcttttttca taaatcaatc actcctttta
    10141 tttttataaa ccaagcatat cttgtaagcg ttttctattc aaaatatata tttccgtagc
    10201 attgcggaaa aaatgtgtat catatcttga ataataattt ctggcaccgt gatatactag
    10261 tgatagaatt ttttcaggag gggatttagt gagtatcaca gtgaatattt tgtatacaag
    10321 tacaaatgga caagccagaa aatttgcaga agaaatggtc gaaagaaaca ttgtcaaagc
    10381 gattcgtgca gaagaaggaa acgagaaata tgactattac tttccactag aagatccaga
    10441 atctctttta ctgatcgacc gttggaaaga tgaagaagca atcgaaaagc atcacaaatc
    10501 cgaaatgatg gctcaaataa acgaactccg taaaaagtat aaattaaaaa tgaaagtcga
    10561 acgtttcact gatttaccgt aaaaagatac acatggaatg atagcaattt atcatccatg
    10621 tgtatcttca ctttttcctt attttttatt cgatctcatc taatgattca aaccgtgttt
    10681 ttctcttatt tttcggataa gtatttcagt caccccaata tacatgattc ctaaacatac
    10741 cccaaggata ttctcaaaaa tgcggatcgt tactgctcct ggaaccccga ataaactggc
    10801 agtgattgcc aaagcaccaa aagaattaaa aactgtttta tatctatagg tagaacaaac
    10861 acctaaagct agaccaccta atataccgac ccagttaaaa ggaataaatt ggctgatacc
    10921 aataaataaa acacatccaa tcaatgagcc aatgattcgg tctactgctc gttctttgaa
    10981 accaatcgac atcaatccat agctagatac aatagaagaa aatgcaaacc ctgcccacat
    11041 caatcgctga aaagggacat actctcctgc aacgatcaaa agactaatgc caaatgcata
    11101 gatgagcatc caaatttttt cctgcgaata catgcctttc aaaaaaatct ctttaaacaa
    11161 gcttttgtct ttgttctttt ctcgatgttt tctgtaaagg agaactgaaa accaacaaaa
    11221 aaacaaaaat aataagctac ttgtttgtgt aaagaaattc ttgttcaacg aatctttagg
    11281 taacgaataa acaataaaga gatatgaaaa gccgtacaaa gaagcattcc ccattttggg
    11341 atcactagcg gttgccatta atatgctgct tagtaaaata aaatgaatca acatgcccat
    11401 aactgaccat ttgatcagct ggacatacgg tgctactaat agtgagaaca tgacgattgc
    11461 taaacctatg atcgaatggg aaatcttata accaaagtca acaaaacgaa tactcatcaa
    11521 catacagaat aaaaccacag cgtatggttt atgcttcgat tcaaataatg ttgtaattat
    11581 aatgataaaa caaatggcaa acaatacgat gaataatgat cgtaaaaaca atgaacaaat
    11641 atacatgcac ttttcttttt tagaagctgc ttctcgtatt ttttgtttta aaataaacgg
    11701 atctagttgt agagactgat aaaaatccac ctgttgtcgt cctttctttt tacttttatt
    11761 ttttacagtc atctatgaca ggttgtaaat actatggaac atcatatggc gttgtcattt
    11821 tcttgtcaac tatttttcct attcatgttt ttataaaaaa acatatcgtt ttcttgtatg
    11881 attaaatcac cacaaaataa acaactgaga gaacgatatg ctttatgaat ggttctatca
    11941 aaaaaattct gagatcaata gacaaaaatt tgatgattac agaggtctct tacgaaacct
    12001 tacagaagaa aaaaacattg atcgttgctg cagttctctt ccctgctcca tgtgtctgta
    12061 gaaattgtgg ttctgctgtg gtagatggaa ataggaaatt aatggtggtg aaaaacaaga
    12121 agaaggaaac cattgtccac tttgaataat ataatcatat gccaatggtt atacgcctaa
    12181 aaaagcagcg ctatacctgt aaaaactgtc gaagccattg gaacgctcaa agttatttta
    12241 tccgtccaag gcactcgatt tcaaatcatg tcagacataa aatcacttct ttactgactg
    12301 aaaaagtgtc gttatttttt atttcgaaaa gttgttaggt atctttgaca accgttattc
    12361 gcacattgaa agagttgaga agctatttac caaagtcttc caaaagaatt ctgtcttgag
    12421 tgttgagggt tgtgaatttc gttctcacgt ttccgttgag gataaactga gttttatttc
    12481 cgcggatggc gagacagaaa aattaatcga gaaaatttac ctacacaaaa aattcctcga
    12541 ttaacaaact atttccttag ctgcactaat cctaaagaaa tagaattctt agttacagac
    12601 atgaacgcca cctactttca gctcaccaaa catgttctac caaatgcgaa attggtgatt
    12661 gatcgattac ttggtttttc cacgcctttc aaggaggcgt aacctttatt cacgagttgg
    12721 ttgaagcttt ccgagacaaa agacccgact tgtttttctc cttattgaca aagagccatt
    12781 aaaactagag aaaaaattac aactgatcgc tgattatttg tagaaagaaa atcaaagtat
    12841 tacccaataa acttgagtca ttaggcaaaa aacactgcga aactcaagaa tgattttcgc
    12901 agtgtttttg tatatttttt attaactagt attactacaa aggtttattt ttcacttctc
    12961 aacgcagcac aaaattgtct ttacttattt actggttggg ttcaaccttc tctactccga
    13021 tactaatatt tgtttgtata ccccttctaa aatcggtagt ttccattcaa ttgatgggta
    13081 ctagttgatt ttaaaaacta tgcttattca attttttatt ttagctcata tatttcgttt
    13141 ctttctaaat tttatactga tgaaaacaag tagcacaccc aatattgaca atataaactg
    13201 gctaatgatg gtttctccag tttttggaag atgcttattt ccactgcttg ttggattgtt
    13261 tcctcttata tctgagctat cagcaacttt tctattctct tttgttaaaa agatcgccac
    13321 ttcttgttct ttttcgatct tgaaagttac tggtgtcgca tccaattcat accccgttgg
    13381 cgcttgagtt tctaccagct gatatgctcc tggtgctaag ccatctatcg ccagttttcc
    13441 atctgctcct gtggttagac ctgactgcag aacggttcca ttggcatctt gtaactcaaa
    13501 aacagctcct tgcaggactt ctccgctttg gtcatcgatc tttgtcaaca cgacaccgcc
    13561 tggtgtaagt cgattttctt tcgtcaattc taccaccgct gtctgacttc tttcgatctc
    13621 aaactcgatt ggtgtcgcat ccaattcata ccccgttggc gcttgggttt ctaccagctg
    13681 atacactcct ggtgctaagc cgtcaatcgc cagttttcca tctgctcctg tggttagacc
    13741 tgactgcaga acggttccat tggcatcttg taactcaaaa acagctcctt gcaggacttc
    13801 cccgctttgg tcatcgatct ttgtcaacac gacaccgcct ggtgtaagtc ggtttgcttt
    13861 cgtcaattct accaccgctg tctgacttct ttcgatctca aactcgatcg gtgtcgcatc
    13921 taattcatac cccgttggcg cttgagtttc taccagctga tatg
//
LOCUS       DAYYGO010000071        13926 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000071 DAYYGO010000000
VERSION     DAYYGO010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.073"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(34..909)
                     /locus_tag="ACHA5T_001849"
     CDS             complement(34..909)
                     /locus_tag="ACHA5T_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8887414.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            1057..1389
                     /locus_tag="ACHA5T_001850"
     CDS             1057..1389
                     /locus_tag="ACHA5T_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFM8887415.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            1501..2709
                     /locus_tag="ACHA5T_001851"
     CDS             1501..2709
                     /locus_tag="ACHA5T_001851"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFM8887416.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            2863..3318
                     /locus_tag="ACHA5T_001852"
     CDS             2863..3318
                     /locus_tag="ACHA5T_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887417.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            3680..3955
                     /locus_tag="ACHA5T_001853"
     CDS             3680..3955
                     /locus_tag="ACHA5T_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887418.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            3968..6046
                     /locus_tag="ACHA5T_001854"
     CDS             3968..6046
                     /locus_tag="ACHA5T_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFM8887419.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            6209..8092
                     /locus_tag="ACHA5T_001855"
     CDS             6209..8092
                     /locus_tag="ACHA5T_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8887420.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            8104..9051
                     /locus_tag="ACHA5T_001856"
     CDS             8104..9051
                     /locus_tag="ACHA5T_001856"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFM8887421.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            9071..9598
                     /locus_tag="ACHA5T_001857"
     CDS             9071..9598
                     /locus_tag="ACHA5T_001857"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFM8887422.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(9661..10314)
                     /locus_tag="ACHA5T_001858"
     CDS             complement(9661..10314)
                     /locus_tag="ACHA5T_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFM8887423.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            10446..11288
                     /locus_tag="ACHA5T_001859"
     CDS             10446..11288
                     /locus_tag="ACHA5T_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8887424.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            11446..12342
                     /locus_tag="ACHA5T_001860"
     CDS             11446..12342
                     /locus_tag="ACHA5T_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM8887425.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            12345..13058
                     /locus_tag="ACHA5T_001861"
     CDS             12345..13058
                     /locus_tag="ACHA5T_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFM8887426.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            13078..13596
                     /gene="msrA"
                     /locus_tag="ACHA5T_001862"
     CDS             13078..13596
                     /gene="msrA"
                     /locus_tag="ACHA5T_001862"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM8887427.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            13593..13820
                     /locus_tag="ACHA5T_001863"
     CDS             13593..13820
                     /locus_tag="ACHA5T_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFM8887428.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
BASE COUNT         4799 a         2398 c         2874 g         3855 t
ORIGIN      
        1 atctttcctc atagcctatc tttgtctttt ttcttatagt tttttcagta atcttgtctg
       61 tggcttcaaa taagtaaagc cttgcccttc cacttcatgc acattgatga ttgaaaggaa
      121 agctttttcg tcaatctcgt ggacgatcct cttagcttca gttaattcac tagagctaac
      181 gaccatatag atgatttttt ttgaattacc agagaatgcg ccttctccag aaaggaaagt
      241 cacacctctt tgcagaccgg tcatcagtag tggtgcaatc tcttcgcttt tgttggagac
      301 gacaagtaga ccttttgcag aatatccgcc atctaagatc gaatcaatca cacgactgaa
      361 aacatacgaa acaatcaacg tatacatcat acgtttcaag tcgatatagg ttaatgaagc
      421 aagtaagacg atgatatcaa atgccaatag ggaacgcccc atagtgatgc cgtaattttg
      481 ttctaaaatc cgagcgataa tatcactgcc gcctgttgtt ccacccatgc ggtagacgat
      541 cccgcttcct acacctgcaa ctaaacccgc aagtagagaa acgatcagta gatcatgctg
      601 taaattgatt tctagtggaa ccctttgcca gatccataaa aagaatgaca aagaaagtgt
      661 acctagaaca gtataggcca aggaacgttt accgaggatc tttcctccta gtaaaatcaa
      721 tgggatgttg attaataaag tcgtataagc tggatcaata ccaaataaag ctcttaagat
      781 caaggtgatc cctgagactc ccccttctgc taaatgatta gcgatgttca agtaaacaag
      841 accaaatgca taaatacttg ttcctataat gataaaaaga acttctttta ctgtttttcg
      901 tttgaccata tgaattccag ctttcaatta tgtattttca cagaatttcc atttagaaat
      961 tccttcctaa atgtagcatg gaaattcgat tttctcaatt gctcttatct aggatttgcg
     1021 ttaaagtttt ggtaagatag ttttggaggg aaaaagatgg ataaaaagcg ttctttatac
     1081 agtatgcagc aggaagtgga tgaatatatc cagcaattca aggtggggta tttctctcca
     1141 ttagctcaga tggctcgatt aactgaagaa gtaggtgaat tggcaagaga agtcaaccac
     1201 acttatggag aaaaaagcaa aaaggcttct gaaccagtta actcggtagc agaagaactt
     1261 ggtgatgttt tattcgttac gatgatcatg gctaattcat tgaatatcga cttaactgaa
     1321 gtttttgaaa aaaacatgga aaaattcaat caaagagacc ataatcgctt tgagcgtaag
     1381 gacgagtaaa caagatgaag tggtcctggc cttgaagact ctgaaacgga cttgtcaaac
     1441 agtcaccatg caactgagtc tttatctcag gacaagaaac acgcaggaaa ggttggaaaa
     1501 atgaaattaa aacaattacc tctggaatac caaaaggcaa ttccagttct gaaaaaatta
     1561 gagaatgcag gatatgaagc ttattttgta ggtggaagtg ttcgtgatat cttgctgaac
     1621 cagccaatcc atgatgtgga tatcgcaaca agcgcatttc cagaagaaat caaacagctt
     1681 tttccaaaaa cgatagatat tgggatcgaa catggaactg tgttggtatt agaaaatgat
     1741 gaacaatatg agatcaccac atttcggacg gaatcaactt atcaggacta ccgtagacca
     1801 gatcatgtcg aattcgttcg ttcgttggca gaagatttga aaagaagaga ttttacgatc
     1861 aatgcgtttg ctttgaaaga agatggagaa atcatcgatt tgttcgatgg tctgcaagat
     1921 ctagaagaac agatcttgcg agctgtaggc aatcctcatg agcggttcca tgaagatgcc
     1981 ttacgaatga tgcgcgggtt gcgttttgtc agtcaactag ggttcaaatt agaggaagaa
     2041 accttctacg ccatcaaaga aaaccacgag ctattaggta aaatatctgt agaacgtata
     2101 aatgtggaat tcatcaagat gctcttggga aatgaacggg ccgcgggaat ccatgcattt
     2161 attgaaacag agtgctacat ttgctgtccc ggtttgaaag aagaaggcga agcactttta
     2221 cgttttgctg aattaccatc cgtcccattg aaagaagaga gccaagcatg ggcattattg
     2281 atcagccaac tggatctaaa agaaaatgag attcgttcct ttttgaaatc gtggaagtgt
     2341 tctaaccaaa tgattaaaga cgttcaagca cttgtccaag gactacgaca acgagaaact
     2401 ggagctttgg aaccattcga attgtacttc ttaggcaaaa aacatgcact gcaagtagaa
     2461 gaattgatga tctattttga cgaaacacct cagatggatg ccgtaggaga atcctatgat
     2521 cgtttaccta tcaagtcatt aagcgaatta gcagtagatg gtaaaatctt gctagaagaa
     2581 actggaaaaa aacctggtaa gtgggttagt gaagctttgc aattagctga aaaagccgtc
     2641 gtagaacaaa gaataaaaaa tgataaaaaa caggttttgg attttttatc gaaagaaaaa
     2701 ttgatttaaa aaacactaga ctgtatctgc tcagctttgt tggctactta aagtgggcag
     2761 atacgtcatt agaagaaaaa acataagatg tgaccactta cataaatttg tgctaaactg
     2821 aggtagtaca agaataaatt acatcataaa ggagtctttt taatgagtaa agaaatgaca
     2881 gcgctaaaat tttattttag aaatggagaa acctggacga tcaaccgccg tcatatcggc
     2941 gatctttgga tcaaacaaat cacaacaagt tttggacgaa tcaatggcag tgaatttgtt
     3001 gaaatccatc cgtgtgctgg atttaaaatc gaaatcttcc atgaagggga cgcagttgcg
     3061 acacatgata tcaacctagg cggactggaa atgggcatgt ttaatcgcgc attgaaatac
     3121 gaagatatcg agcgaatgga aatcctttac cgaaatggca cacctgatct tgtgtacttc
     3181 ccatatctag ataaaggaac agaaggatta gacaatcaat atcaatcaac aaaaatcagc
     3241 gaaaaaacag gtaacttgta tatcgtgatc aatccagatc aacgtgtaga agatgtatac
     3301 ggggaatttt tcgaataaat cgatttttac gcaaaaatga ggctgtgaaa gagatgtgtc
     3361 ttattactac tgataggctg tctcttccac accgtttatt cagaataagg gactgtgaca
     3421 agatttttgt cgcagcccca tttttttgtt gtgataaaag aacaagaaag gcaggaatgg
     3481 gaaagtaaac atccttttgt cagaaacttt tatctttctt aagttttact tgatatctga
     3541 tactatttgg aatagaatga ggcgttgaaa aatttatgga aatttctaac tagcaataaa
     3601 cgatgaaaca atgaaatatt tttcatccaa aaagaacttt gctataatga tggaagtttg
     3661 tgtataggag atctggaaaa tgagagagag ttttgaacaa caaaaaaaac tgttgcatga
     3721 tcgctatggc gccctttcga tggatgaccg ccgtcaaata ctttgtaagt tgcgtaaaag
     3781 aaatatattg atgtatcgtc agttggaacg tctgaaacat gatctgcttc gtttggagtc
     3841 aaaacgtgta cagtgtgaat tagaggggaa ccaaacacag gttgaagtag tcgaaaccaa
     3901 aattttaaag aaaaaagaac aatttttaaa aatgcttaca caaaataaaa aataaagggg
     3961 atggacgatg gaatttgctg agttagttat tgtatttgca gtaacgatca ccttatcgaa
     4021 catagctagc cgcattttgc caatgattcc agctccactg atccaaattt tccttggagt
     4081 ggtattaggt ctgacagagt ggggacaatc aatcgatttt gaacctgaat tgttcttagt
     4141 tatgattatt gcaccattgt tatttagaga aggagaaaaa gcagatattt cttcgattct
     4201 caaaaatttc gggacaattt tgttcttggc atttggagga gtcattttga cccttgtcgg
     4261 ggtaggtgcg acgttatcat tcctgttgcc aagtgtccca cttgctgcct gctttgcttt
     4321 tggagcagcg ttaggtccga cagatgcggt agcagtcagt tctctttctg gacgagtgaa
     4381 tatccctaaa aaggcgatgc atattttgga aggagaaggt cttttgaatg atgcatctgg
     4441 tgttactgcc ttccagtttg ctcttggtgc gctgatcacc ggatcttttt cagcagtaaa
     4501 cgcggggatg agtctagtag tttctagtat tgggggagct ttgatcggct ttttgcttgt
     4561 ctggttcaaa caaaaaataa ttcatttgat cgaaaaagca tcagcacaag atgtcacagg
     4621 gtatctgctg atcgaacttt tgcttccttt tctagcctat gtattggcag aggcatttga
     4681 tgtatctgga atcattgctg cagtggcagc aggtattctc caagcttctg gttttaggaa
     4741 aatctctgtg tttgatgcag aattgtcgag cttgtcccac agtacatgga caactattgc
     4801 ttttacactg aatgcattag tatttatatt tttaggaatc gaattgacgc aagtgttctc
     4861 gccagtatgg ggagacggtt tatatccaaa cggtctttta ttagcgatta tcgtattaat
     4921 ctctgtcatg ctttttgtga tcagattcat ttctatcagt cttttttatg tattcaaaga
     4981 tggcagtaaa aagttcaaaa aacagttgaa cgagatcttg atcttaacat ttggcggtgt
     5041 aaagggaaca gtcagtttgg ctacgatttt tattttgcca ttttcaatca ataatatgat
     5101 gttttaccag cggtctttgc ttttgtttct gacggcgggt gtcatcttag tgactttggt
     5161 catagggatc attgtcttgc ctatgctgac ggaaacagag gaagcgaaaa gcacggattt
     5221 gaatgctttg atgattttag aagaggtcgt tgaagttttg cgaaaagaaa taaaggaaat
     5281 tgatcgtaat acaaaagaat ttttagccac tgaagcagtg atagaaaatt atcaagaacg
     5341 gatacgtgat ctgtacttgg aagatttaac cgatgatgaa aagcaagaag tacaagaaat
     5401 acaagcactg attttgtcga tcgaacggga tggattggat gaaagttatc gttcgggcaa
     5461 gctttcttct aatggttatc gtttttactc ccgttttctt tctcgattcg aacattctat
     5521 cacaagccag attttgtctt ttatcggctt ttggttcatt gttgtccgta ggttaatgcg
     5581 aatcgttctc catccgaaaa tgttttggca aagaagatat gctgatcgtc agacatttat
     5641 tagtgatgaa gatatccaag aaatcagaga gacatatcaa aaaaatactc agttgattat
     5701 tgaaagttta gataacttga ctgatattta tgatgaaacg atggttcaat tttttatcca
     5761 gcaacgcaaa cttgaaggaa taaaaatgaa ctcggggaat ttgatttctt cgtggatgat
     5821 ccagcaagat agtctgttta cgaaaaaaat gctaagaggt tattatcttg aaagaaaaat
     5881 cattgatgaa tacgaagttg ctgaaaaaat cacgacgttt tctgcgaaca gctatcgcag
     5941 gaatatcaat ttgttagaat cttatacgat gaacaaacca tctgataact tttctttccg
     6001 atttgcgttt cgagcaaagt caaaaaaaac gagtccaata aaataaaaag gtaaaaattt
     6061 tcaagtttta taaaaaacaa aacatatcag aacaggatcg aatcggtcct gttctttttt
     6121 tgagaacaca aaaagaacaa ataccaaaaa aattttcctg aagttcacta tttttatgat
     6181 acaatatcca agataaggag ggaacatgtt gaaagaatta aaagtaactg acctcaaaaa
     6241 aacttatggt gaaaaagatt tgtttgacca gatttctttt ttggtccacg aaaaagaccg
     6301 tatcgggttg attgggacga acgggacggg taaaacaagt ctcttaaata ttattgccgg
     6361 tatcgatagc agcgacggtg atcggcaaac agtgttttat ccaactgatt accgtattgg
     6421 ttatctttct caagcagctg aattttccga tgaattaact gttctccaag cagtctttca
     6481 aggaaacagc cccttgatcc aaactgttcg agcatatgag gaagcattga tcgaactagg
     6541 ggaaaacgga gaagaccctg atgtgcaaaa acgttatgct aaagcagaag aacaaatgaa
     6601 taaagaagat gcttggacta cagataccaa cgcaaagatt attttgcaaa aattaggaat
     6661 cagtgaatta gataaaaaaa tcagtacttt atcaggtggt cagaaaaaac gagttagtct
     6721 ggcgcaagtg ttgattgatg aacccgattt actattacta gacgaaccaa caaaccattt
     6781 agattatgaa gcgatcgagt ggttggaaaa ctatttgaaa caataccgtg gcgcactatt
     6841 gatggttacc cacgatcgtt attttctaga tagagtagca aaccgaatct tcgaactttc
     6901 atttggaaaa ttatacgagt ataaaggaaa ttatgaagct tacgtcttag aaaaagcaga
     6961 acgtgatcgt gtagaagtag aacaagaaga aaagcgcaag cggctttata aacaggaatt
     7021 agcttggatg agggctggcg tacaggcacg tggcaccaag cagcaagcac ggatcaaccg
     7081 tttcgaagat ctaaaggaga acttgtacca agtaaaccaa gaagatgatt tagagctgaa
     7141 cctagcgaca caacgtttag ggaaaaaagt actggaaatc aaagatggca gttaccgtat
     7201 aaatgaccaa acactgctag aacatttaga tttattgatt caatctcggg aaagactagg
     7261 tattactggt aaaaatggtg cgggaaaatc gacattgctt aatattctag ctggcagaat
     7321 tcccctggat agcggtacaa tgtctatcgg tgaaacggtt catcttgcgt attatacaca
     7381 agaaaatgaa gaaatggcac cagataaacg gatgattgct tacttacaag aagctgctga
     7441 agaagcaaaa acagcagatg gcagtcaaat cggagtagca gaattacttg aacgattttt
     7501 atttccaaga tttatgcatg ggacactgat aggcaaactt tcaggcggag aaaaacgccg
     7561 gttatttttg ttgaaactgc tgattcagca acctaatgtt ttattattgg atgaaccgac
     7621 caatgatttg gacatagcaa cattgacgat tttggaagat tattttagaa gttttccagg
     7681 tgctgtgatc actgtttccc atgaccgtta tttcttggac aaggtagcag ataagttatt
     7741 agtattccaa ggaaacggaa aacaagaact ttattacgga aacatgtcca gttatttact
     7801 gaagcaaaaa gagacccagc agcctgctga aaaagcgaaa ccaaaaatac agagcaagga
     7861 acctgctgaa aagaagaaat tatcttacat ggaacaaaaa gaatgggaaa cgattgaaga
     7921 tgagattgca gaacttgaag aaaaaatctc attgctccaa gaagagatga atcatcaagg
     7981 agataatttc actcgattac aagaattgca aaacgacgta tctgaaacag aagcacaact
     8041 tgaagaaaaa atggcacggt gggaatactt gagtgaatgg gtagaagatt aggaggaaaa
     8101 aggatggaac aagcgtatct tgacttagga agaaaattgt tggaagaagg gcatcgtaaa
     8161 gaagacagaa caggcacagg cacattaagc ttattcggat accaaatgcg gtttgatctg
     8221 caaaaagggt ttcctttgtt aacaacgaaa cgagtacctt ttggattgat caaaagcgaa
     8281 ttgctttggt ttttaaaagg agatacgaat atccgctatc tattacaaaa taaaaaccat
     8341 atttgggatg aatgggcatt tgaacgctac atcaaaagtc cagactataa tggaccagat
     8401 atgactgatt ttggccatag atgtttgaca gatcctgaat tcaatgaaga atataaaaag
     8461 cagagcaagc ttttttgcga gaagatccta accgatgatt cctttgctga aacatacggc
     8521 gatcttggac acatctacgg ctatcaatgg cgtcactggg aaacaaaaga cggtggcttt
     8581 atcgatcaaa tcaaacaagt gatcgaagca atcaaaaaga ctcctgattc tagaagattg
     8641 atcgtttctg catggaatcc ggaagatgtt cctagcatgg ctttgcctcc ttgtcatacg
     8701 atgtttcaat tttatgttca agagggacgg ctaagctgcc aattgtatca acgcagtggg
     8761 gatgtgtttc taggcgttcc gttcaacatt gcaagttatg cgttgctgac tcacctaatt
     8821 gcacatgaaa caggtttagc ggttggcgaa tttgttcaca cattaggaga tgctcatctt
     8881 tatatcaatc atatcgaaca aatgaaagaa caattgagcc gagaaatacg cgcatttccg
     8941 actttagttt tgaatcagga aaagcagtca gtatttgatt ttgagatgga agatatccaa
     9001 ttagaaggat atgacccaca tccggcgatc aaagctccta tcgctgttta gaatcagaaa
     9061 ggatgagaaa atgttagctg caatttgggc acaggatgaa aacggattga ttggaaaaga
     9121 agaccgtctt ccttggcgtt tgccaaatga tctgaaattc ttcaaacaaa tgacagaagc
     9181 aaatacacta gtgatgggac gaaaaacatt cgaagggatg ggtagccgtc cgttgccgaa
     9241 tcgtcagacg attgttttga cgagagattc ttcctataaa gcagaaggcg tgcatgtcat
     9301 gcatagtgtg gaagacgtgc ttgctctgga aaaagaaaca gacggtatct ttttcatcgc
     9361 tggagggtca gctgtttatc aagaatttct gccattttgt acgatattat accgtacagt
     9421 catccatcat gtattcgatg gagatgctta ttttccacct gttgactggt ctgattggtc
     9481 actgatcaat actagtcaag gagaaatgga tgaaaaaaat cgttatccgc atcaatttga
     9541 aacgtatcag cgtaatgaga atcctgtaaa aagcgggaca ggcgaattga cgatataaaa
     9601 aagctatgga tagactagct gttatttaga gctgtcccat ccatagcttt atttttttta
     9661 ttaagtaaag aataaaacag agaagtacat aaaacctgca cctaacataa caaacaaatg
     9721 ccaaacaaca tgcatatagc ggatagaatt caagctataa aagaatgcgc ctatcgtgta
     9781 agaaagcccg cctgctacca tcaaaataac accagtcatt ccaagagagt gataaagagg
     9841 aacagcagcc acgacacaga gccaccccat aaaaatatag atgatcgtag atacttttga
     9901 tactctctcc cgtttatgca acgtcaacgc tttatatata atgccaagaa tcgcaaacaa
     9961 ccagattaat acaaataaag tccaacctag ccatcctcgg atactcagca aacaaaaagg
    10021 agtataactt cctgcaatca aaagaaaaat cgaactatga tcgaatactt ggaacacctt
    10081 ttttgctttt gtaaaaatca atgaatgaaa caaagtagac gttaaaaata aaagaatcat
    10141 catcgatcca tagatagcat aagagacaat atggagggag gaatgcaagt gggcaccttt
    10201 tactaaaaga accactagtc ccgcgatact caatccagtc ccaattccat gggtgactgc
    10261 attaaacact tcatttaata tttcatacgt tcgattaaat ttcgtttttt ccatgaaaac
    10321 ggctccttta ctaggtgtaa ttaatgaaaa atcattattt gtctgttata ctaaaaatag
    10381 aaattgtctt acatgtagtt taacatgaat aaggcatctt gttaaagaaa gagagagaga
    10441 aaataatgac aaatgtaaaa atcgtaacag attcttcatg tacgatggaa aaaaatgtac
    10501 gcgatgcttt aggcattcgt gtcatgccgc tatccatcat gatcgacgga acagtatatc
    10561 ccgatgatga ccaactagac ggcgaaagat ttatggaaat gatggcgaaa gcaaaaaatc
    10621 tgcccaaaac aagccaacca ccaattggag aattcatctc gatgtatgat gagctaggag
    10681 aagacggcag tgaagtgatt tcgatccata tgacaaaagg cttgagtgga acagtagaag
    10741 cagcaagaca agccagtaat ctaacaaaaa cgaaagtaac ggttatcgac agtgatacca
    10801 ctgaccaagg attatctttt caggtaattc gagcagctga attagcaaaa gaaggtaaaa
    10861 cagtagagaa aatccttgaa gaagtcaaaa aagtacaaga caatacaaaa ttatacatcg
    10921 gaatatccac actagataac ttggtaaaag gtggaagaat cagccgaaca actggtttgc
    10981 tgtctagtgt tttgaacata cgcgtggtga tgaatttcgc ccattctgaa ttaatccctg
    11041 taactaaggg gcgaggaaag aaaacgttcg ataaatggtt taataatttg aaaaatgaac
    11101 tgaaagaatt gccaaatgtg cgacaaatcg gtatttcaca tgcggatgca aaagaattgg
    11161 cgcaagaatt caaagagggt ttgcaagaat tattcccgga catgcatatc cctgtactgc
    11221 atacgaatcc ggttatcgct acgcatactg gtaaagacgc gtttgcaatc atgtattatg
    11281 tagattagtc gaaaagagga taagccctca gcctgaaagg cttgcagctt attctctttt
    11341 tctttataaa attaggaaaa taattgtttt cttttttcat aaagactacc tttttgataa
    11401 aatttttgat aacattagaa ccaatcgaat acaagaggtg aaaaaatgaa attaacgaaa
    11461 cgacatttga tcctcgtttt gttcccatta ttaattgcga tcatcatcta tgcaggttta
    11521 gctgcttcta taccgaaagc tggcccacta atgaaaccag taactactgt gagagaagat
    11581 ccaaataaaa aagaaaaact tcactacata gctatcggag attcgctgac tgaaggagta
    11641 ggagatcaaa ccaagcaagg aggattcgtt ccccttgtcg caaatgatct aaaagaccgt
    11701 tataatttga ccgcggttga aacagaaaac tatggtgtaa atggtgaacg aagtgaccag
    11761 atcttaaagc gagtaaaaac aaaagaagaa attcgaaaca atatcaaaac agctgatttt
    11821 atcacactga ctgttggcgg taatgatttg atgaaagtta tccaaaataa tttattcggg
    11881 ttaacggtca attcctttaa aaagccactg aaaaactatc aggaaaatgt cacagaacta
    11941 ttggaagaat ttcgatccct caataaaacc gcacctattt acattttagg catctacaat
    12001 ccattttatt tgaactttcc tgagatcact gacatgcaga caattgttaa caattggaac
    12061 aaaggaacag aaacggtgac aaaaaaaatg ccgaactgct actttatccc aatcaatgat
    12121 ttgctttatc aaggattgaa tcaggaagta ggtgtcacag aagaaggaac agatcaaaca
    12181 actagcacga cagaaaatct aaatggcatc aaaaataatg tgctctatga agaagatcat
    12241 ttccatccaa ataatttagg ttatcaattg atggcaaatg cagtgagaga tcagctgatc
    12301 gagacacaaa accaatggtt agagaaggag gatgatcaat aaacatgaaa acaagatctg
    12361 acgtatcttc aaacaaaaca aagaaaacaa attactggaa attcgccttc cttatccttc
    12421 ttggggtagt acttggttcg accgtatttt taggcagccg tatttttgct aatcgggaac
    12481 cagaactccc agaaatacca gctcttacgg aaagacaagg agatcctgtc ttaacgatca
    12541 attcaaataa agaaaaagtc aatcaaatca tcagcttttt ccttagtgag tatcaaaaag
    12601 atagtgatat tgaatataaa ttttatttgg aaaatgaagc actactcaat ggtactttcg
    12661 aagtactagg ttttcctatc gatttctatt tatattttga tccgtatgtg atggaaaacg
    12721 gaaatgtcca actaaaagct agaagtcttt ctatcggaac attgagtttg ccaatcaaag
    12781 atgtgatgaa tatgatcaag agaaactata aattaccaga ttggatcgaa gtcaatacag
    12841 atgatctgac tatcatgctt aggttggatc aattccgaat gcaaaacggg atgtatatta
    12901 aagcagataa aatcgactta gtaaatgatg acattcggtt tagtctatat ttgccaaaag
    12961 atacctctga tgcttcaaac aactcaaata ccaacaaaac tagcaccagc agtactgaaa
    13021 gcaagacaaa agaaaataaa aaatcatcgg atgagtagta ttagaggggg aatcataatg
    13081 gaacgagcaa tatttgcagg aggctgcttc tggtgtatga tccagccttt cgacacgctt
    13141 cctggtatcc atacagtgat gtcaggatat actggagggc atgtaccaga tccgacttat
    13201 gaacaagtaa aaagccaaac gactggacat acagaagcag tggagattat ctatgatcca
    13261 acattgattt catatgaaca attagtagaa ctttactggc agcaaactga tccaactgac
    13321 gctttcggac aattcgagga tcgcggtgac aattatcgcc cggtgatctt ttatttgaca
    13381 gaagaacagc ggatcattgc tgaaaaaagt aagaaacagt tagctgaaag tggacgtttc
    13441 gaacagccaa tcgttacaaa aatcgaacca gcccaaccat tttatctagc agaagaagac
    13501 catcaagagt attataaaaa gcatcctgaa aattttaaaa gaaaccatgc acgccgggct
    13561 gcctttatcg aagaaaattg gaggaacgaa gggtgaatcg aagctttttt cattatttaa
    13621 tgacactgcg aggcggtggc acacatgatg ctttatctgt tttcgctaca gaagccggta
    13681 aagacatcca atttcctaag cacagctcta gctacgaaga aatttctaat tatctggaac
    13741 tgaatgtcga ctatttgccg tccatggata tctttgacga agcatgggaa aaatatttag
    13801 aaaataatca tgacatataa gagatagttt tgtactttat gatcaaaaaa gttttttgtc
    13861 caatagccta attgaaagaa ttatttttat ttcagctatc tagtcgcatt tttttgatat
    13921 ttttct
//
LOCUS       DAYYGO010000072        13808 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000072 DAYYGO010000000
VERSION     DAYYGO010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.074"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(162..2159)
                     /gene="tkt"
                     /locus_tag="ACHA5T_001864"
     CDS             complement(162..2159)
                     /gene="tkt"
                     /locus_tag="ACHA5T_001864"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFM8887429.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2261..2512)
                     /locus_tag="ACHA5T_001865"
     CDS             complement(2261..2512)
                     /locus_tag="ACHA5T_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFM8887430.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2659..3282
                     /gene="lexA"
                     /locus_tag="ACHA5T_001866"
     CDS             2659..3282
                     /gene="lexA"
                     /locus_tag="ACHA5T_001866"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFM8887431.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3462..3680
                     /locus_tag="ACHA5T_001867"
     CDS             3462..3680
                     /locus_tag="ACHA5T_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887432.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3969..4481)
                     /locus_tag="ACHA5T_001868"
     CDS             complement(3969..4481)
                     /locus_tag="ACHA5T_001868"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFM8887433.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4495..6792)
                     /gene="recJ"
                     /locus_tag="ACHA5T_001869"
     CDS             complement(4495..6792)
                     /gene="recJ"
                     /locus_tag="ACHA5T_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFM8887434.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6934..7377)
                     /locus_tag="ACHA5T_001870"
     CDS             complement(6934..7377)
                     /locus_tag="ACHA5T_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFM8887435.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7378..8166)
                     /locus_tag="ACHA5T_001871"
     CDS             complement(7378..8166)
                     /locus_tag="ACHA5T_001871"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8887436.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8163..9104)
                     /gene="rnz"
                     /locus_tag="ACHA5T_001872"
     CDS             complement(8163..9104)
                     /gene="rnz"
                     /locus_tag="ACHA5T_001872"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFM8887437.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9281..9868)
                     /locus_tag="ACHA5T_001873"
     CDS             complement(9281..9868)
                     /locus_tag="ACHA5T_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFM8887438.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10089..11402)
                     /gene="obgE"
                     /locus_tag="ACHA5T_001874"
     CDS             complement(10089..11402)
                     /gene="obgE"
                     /locus_tag="ACHA5T_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFM8887439.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11690..12700
                     /gene="gap"
                     /locus_tag="ACHA5T_001875"
     CDS             11690..12700
                     /gene="gap"
                     /locus_tag="ACHA5T_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFM8887440.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12785..13720)
                     /locus_tag="ACHA5T_001876"
     CDS             complement(12785..13720)
                     /locus_tag="ACHA5T_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8887441.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3808 a         3096 c         2435 g         4469 t
ORIGIN      
        1 ctcaagaaac acaaactaat ccgaagaatt ctttgttttg tcatttaaat gtaacatagc
       61 cgaaaacgaa aagtaatatt gatttattct tctaacacat aaagaacagc tgtctccaac
      121 ttgcagacag ctgttcttat cagtcaaaca attgcttgga tctatgatag ttgattgtac
      181 gtattcacga cattttctac tgtaaatcca aattcttcta aaattttagt gccaggggca
      241 gatgcaccga aatgatcgat tgtaatcgtt gcgccctctg ttcctacgta gcgttcccat
      301 ccaaatgatg cagcagcttc gatagccaca cgtttcttca ctgatttagg aagtacactt
      361 tctttatatt cagaagattg tttttcaaag aggtcaaagc ttggcataga aacaacagaa
      421 acatctttgc ctttttcagc caattctttt tgagctttca cagccaaatc aacttcagaa
      481 cctgttgcaa tcaaaatacc ttctggtgtt tctccttgtg atggagaaag aacatacgcg
      541 ccttttttca ccatttcatc tgctacttct tttgtagaag gcaatactgg aaggttttga
      601 cgactcaata caaggatagt aggcgcatct gttgtctcca tcgctacttt ccaagcagca
      661 cgtgtttcat ttccgtctgc cggacgaatc acttgaacac caggcatgca gcgaacagat
      721 gctagttgtt caatcggttc gtgcgttggt ccgtcttctc cgacagcaac cgagtcatgt
      781 gttaagacgt aaacaacagg agtatgttgg attgctgcta aacgaactgc tggtcttaga
      841 taatcgacaa agacaaagaa tgttccaccg tagatacgag ttccgccatg aagttggata
      901 ccattcatag ccgaagccat tgcaaattcg cggacaccga accagatgtt gcgtccttca
      961 taatgttcag gagtgaaatc tttatctgca gccaccattg tattattgga acctgacaag
     1021 tccgctgaac caccccagaa gcttggtata gctttcgata attcttggat gacttcttta
     1081 cttgaaacac ggcttgcttg actgcttcca acttcataag ttggcaattc tgcatcccag
     1141 ttttctggta gtttgccatc aaaagcatct tcaaattgtt gtgctaattc aggataagct
     1201 tttttataat tggcaaacat ttcattccat gcatcttcag ctttttgtcc ttcttctatc
     1261 atcgtttgat ggaagcgggc agctacttct tctggaacag tgaaatctgg atactcccaa
     1321 ccataaactt ctttagccat cttgatgcca tcttcaccaa gaggagcacc atgaacagca
     1381 gatgttcctt ctttaggagc accatagccg atgactgttt ttacttcgat caatgttggt
     1441 ttgtctgttt cagcttttgc ttcttcaatc gctttagaga ttgcttctag gtcatttccg
     1501 tctttcacta agatatgttg ccagccatat gcttcatagc gagcaccaac gttttctgtg
     1561 aaagcttttg aagttggacc gtctagtgaa atatcgtttg aatcgtataa aacgatcaat
     1621 ttccctagtt tcatatgacc tgccatcgaa cttgcttctt gagatacccc ttccatcaag
     1681 tctccgtcac cgcatattgc atacgtatag tgatccatca cattaaagtt ttctttgtta
     1741 tacgttgctg caaggtgcgc ttctgccatt gccataccga cagccattgc aatcccttgt
     1801 cctaatggcc ctgtagtggc ttctacacca tctgtatgat gtacttcagg atgtcctggt
     1861 gttttagaat cccattgacg gaattgtttc aaatcatcga ttgtcacttg atagccagct
     1921 aaatgaagca agctatacaa cattgctgac ccatgtcctg ccgaaagaac gaaacggtcg
     1981 cggtcagccc agttttttga tgtcgttgga tttactttca ggtgttttgt ccataacgca
     2041 tatgccatag gtgctgcgcc catcggcaat ccaggatgtc ctgagtttgc tttttggact
     2101 gcttcaatac ttaatgtacg aattgtgtta acgccaagtt ggtcaatttt gtcaaacaaa
     2161 ataacccctc ctattaattt aggttttttt tacctttatt tcttacactt tcattctata
     2221 agatgtcaca cgaaaaagca atctttttca gtatttttta ctaaaagttg tcatttcggt
     2281 tgtgtaagcc ttttttcttt tggatttctt ttaatttttc cggggtcacg tcattgccct
     2341 caggatcgac gactttcatt ccttcgatat gatgacgcat accggaacga aaagcttcca
     2401 aatactcttt gcgcaagttg atttgttctt gttcttcggc ttttgagaga ccattttcct
     2461 tctttttacg tgcaagttca ttgatccgct ctattttttc aggtgacagc atgctcagcc
     2521 ctccttttac tctccttcat attatataaa aactgaaaaa aaaacaacta gataaactaa
     2581 ataaaatacg aacattcgtt tgatttagcg ctttttatat ggtactattt atttatcaag
     2641 tgaaagaagg tgtaccttgt ggcccgacaa actgaatcaa gacaaatcga agtcttacga
     2701 tatatccatg aacaagtaac ccaaaaagga tacccgccga ctgtccgtga aatcggggaa
     2761 gctgttcagc tttcttctac ttctactgtt cacggtcatc ttgcacggct tgaaaaaaaa
     2821 gggtttatcc aaagagatcc gacgaaacct cgtgcaatcg agttgacaaa acaaggttta
     2881 gaaaagattg ggattcgtcc aacaactatt ccagtacttg gcgtcgtaac agctggagaa
     2941 cccattttag cagtagagga tgcttcagac ttctttccaa tccctcctaa tctttcttcc
     3001 gaagaaggca gtctctttat gctgacaatt cgtggagata gtatgatcaa tgctgggata
     3061 ttggatggtg acaacgtgat cgttcggaag caagaaactg ctcaaaatgg cgatattgtc
     3121 attgcaatga ctgccgagga cgaagcgact tgcaaacgat tctataaaga aaaaaacttt
     3181 ttccgtctgc agcctgaaaa cgacttgctc gaaccaatca tcttagatca agtatctata
     3241 ttaggacgag tagtaggact ttatcgtagt catatctatt aattgctact aattaaaaga
     3301 aaacttttta aaaagagttt gagacgttgg tctcaaactc ttttttcgcc cgggttcttc
     3361 ttctttcaaa tttttatcac ttccctcttt acaaaccgaa caaatattcg tatactaaat
     3421 atacgaacaa ttgttcgcat aaattaaaga ggtgagaatt catgaaatca atgagacgtc
     3481 ttggcttagt attaactgtt ttgtttttag gtattattat tggtaatttc ggcatacgtc
     3541 cagctttact tgtgtttgtt ccgttattta ctttgtggtt catgttgtgg gatgaaaaga
     3601 agtaccgtca atctgagcgg cgcagaattc atgaagatca tttctatcat gagccctact
     3661 acagatccag gcaatcataa taaggattga ctgctaatta ttcaaaagac aagaagttaa
     3721 acttttacgg gggtgtgaca aaaatcggtc acatcctttt tttcgaataa acggtgttct
     3781 aaagccagtt tttcggtatt aagtcaaatt gcggaaaaag tgatttttta aacttctact
     3841 ggtctttgct tgtttccatt tataaaaaaa tagcgaccct caaaagtcag atagagaatc
     3901 tgacttttga gggtcgcttt aaaatcgctt ctttaagaat aacatcaaga atagcttctt
     3961 ttttctgctt aatattccat taatgtaatg atgtcatatc ctttgatttt gtcgcgtcca
     4021 tggagatcca tcaattcgat caaaaatgcg cagcctacga ctacaccgcc taattgttcg
     4081 atcagttcga tcgtcgcttt gattgttcct ccagtagcta gcaaatcgtc acaaatcagc
     4141 acacgttgac ctggtttaat ggcatcctta tgcaatgtca atgtatctgt tccatattct
     4201 aatccataag tcacttcgat ggtttctcga ggtaacttcc cttttttgcg gactggggca
     4261 aaaccgactc ctaactcata agcaacagga catcctacga tgaatccacg ggcttctggc
     4321 ccaactacca tatcaattcg tttttctttt gcataattta cgatttgttt agtagcttcc
     4381 cgataagcgt caccatctgc catcagtggg gaaatatctc gaaacacaat tccttttgac
     4441 ggatagtcgg gaatactggc aatataatct tttaaatcca ctctttgttt cctcctaggt
     4501 tgttaaccat ttctttatgg taggaatatc gctcaaaagc aaaaattcct caactttgat
     4561 tttattgagc cgtcgctggt ataatctgct ctcagtcaaa ggatgacttt ctgggctagc
     4621 gactttttgt aaaacaccgt tctctattgt aacaaatttc aattcaaaaa acacctgtat
     4681 cataaaaatt aataattttt cagggatacg caaatattga gctattgctt ttattttatg
     4741 acgtatatca attcgttctt gagaatggat caatttaaac aattttgcgt actgttctct
     4801 tgtgcctacc ccattcaaat aggcctcatc aggtgaaatc cccatcagat aaacacgatt
     4861 gaatgaataa gaccgaaaaa cttctttcag atccgctaaa tcatctggac aatccataac
     4921 aactagagag tttggttttc ttttttcaat tattgccttt acagaatcta gtccttcgaa
     4981 aacaatcgca gcttcctgga taatctcagg cacgaatttc aacgtcgaag aatcaaatgc
     5041 taaataaagg ctaccttccg acgttcggct ctgttcacgg aaacgttttg cccgccaatc
     5101 aaatacttgc agaccaccca cagaaaaatc agataccatc agctgcggtt tcttacgccc
     5161 gttccattca ttgatcgaaa gctttcctac cacatctacc tgattattca gcagttcttc
     5221 ttcttgagta ccaaaaccaa aagcgaccgc atcaagctga ctcgatgcat cggaaaggac
     5281 aaatttcaga tgctgttggt ttgctccgat tcttttgata ttttctgctt ggacttgtcg
     5341 aaataaaaac tgaggcaaag gattatccgt accaaatggc gccaatagtt tcagctcatc
     5401 aattcgctct actgttactt catttggcag caatacttca tcaattctca actcaggccc
     5461 tctcatcaag tcaatttgat gatcgactat atattggttc atcttctcct gtagtatagt
     5521 gacattttca cttggcatcg ttaaaccgac tgctgcatga tgtcctccga agtaagtaaa
     5581 taaatctcgc atctggtcaa gcatttcaaa aagattcaag gcttcaatac tacgtccaga
     5641 tcctttagct gatccatctt cttttaaagt aaggacaagc gttggtttac ccgtttcatt
     5701 cattattttc ccagcaacaa tgcccaaaac gccttcatgc catcctcggt ttaccaatag
     5761 atggatatga ttctccttat tgaccatcgc taatgcttct tgtgttatct gctctacgat
     5821 tgcttttctt tcttcattga tctcattcaa tttttttgct tgaacggatg cttcctcttc
     5881 atcaaacgtc gcaagtaacg agacggcagg acgtggatcg cccatccgac cgattgcatt
     5941 tagtctggga gcaatcgaaa aaccgatcgt tgtttcgtct gcgtccctca tttttacgcc
     6001 gctttcttca aatagcgcct gaagtcctat ccgttctgaa tgatgaatgg catcgattcc
     6061 taatgaaaca aggattcgat tctcatcagt caatgaaact aaatcggcga tcgttcctat
     6121 cgctactaaa tccaaaaatt ctgcaggcgg ttcttccaat aatgcagaag ctactttgaa
     6181 tgctactccg actcctgcca aatctggaaa tggatagtgc cccgctggat gacgagggtg
     6241 aacaatcgca taagcgtctg gtaaattctc aggtagttcg tgatgatctg taatgatcac
     6301 gtctacacca gcttgctgag cgtatgctac cgcatcattt cctgctaccc cgttatcaac
     6361 agtgatgatc aactggattc cttcttcgat cttttcttgg tataccgatt ggtttggtcc
     6421 atacccatgc tcaaatcgat ttggcaggaa tacttctact tctgcaccaa gtaattccag
     6481 tgtttctttc attaccgttg cactggtgat cccatctgcg tcataatcac cgtaaacgag
     6541 gattttttct ccactgatta cagcttcttg gattcggctg attgctttat ccatatcgtg
     6601 tatcagatac ggatcatgta attcttgttc ctttggatgt aaaaatcggt tgatatcctt
     6661 ttcctcatgg taacctcttt gccacagtaa cttaccgaca aatggagata atttcagttc
     6721 acggatcatt tggagaaagg attcagaagg ctctgtctcg cttaacttcc aatcaaaatt
     6781 tgcttgtctc actttctcac tcctaactta gttattatag caaaggaaat cagcaaaaaa
     6841 aagcagaaag aaagaataaa aacagatcag atccgctaga cagatgttca gcaggacctg
     6901 atcttttgta cgaccattta tgaaatatcc ctattagtca aaataattga agctttgttt
     6961 gggcgattga gttttttgtt cttccggcgc tttcaattga ctgatttgtg cggttttcgc
     7021 ttgtaactcc gcctgataag cagcttccaa ctccgcacgt tcgtttctaa actcgcgttg
     7081 ctgttcttct tttgcttctt caattttctt ttgaagttct gtttgatatt ctgacatttc
     7141 ctgagtcagc tgtttgattt gctttttttg ctgccatagt gttgccgaag cagtcaaaaa
     7201 tacaatcagt gcaccaatca gcgcagaacc gatgatcact aagatcaatg gcgcagaaaa
     7261 ggaagtgaag ccaaaactta caggaacatt tttattgttc aatacagcaa aaagaacaat
     7321 aatcaaaact aaaacaaatc caagaatcac attgctttgc tttttcaatt ccttcaccta
     7381 ctttttgtta aaaattccgc tggctaaata atcaccaatc gttggaaaca gcgtataaaa
     7441 ttttgatgca acttctaaaa taaacggttg attgatttcc cgttttggat gtctcatatt
     7501 gcggacgatc atttttgcca acttggcagg ttctaaaacg atttgtccga ctttttccaa
     7561 atagcttccg cttggatcag ccttatcgaa gaaatttgtc tcgattggac ctggattcac
     7621 tgtcgtaaca gctacgccaa gtggtttcaa ttctaatcgc aaagcgttgg agaagccaag
     7681 gactgcaaat ttcgtggcag aataaaccgt tgattttgca gttgccattt ttccagccat
     7741 agaggcgata ttaatgatat gaccttgtct tctttctgcc atatcgatcg caaatttttg
     7801 tgtcaaaacc atcattccta agatgttgac ttcaaacata tcgtatgctt ttccaaggtc
     7861 gaatgtaagg aagtcttcga aaatcccaaa acccgcattg tttaccagaa tatcgacgcg
     7921 cccgacttct tccatcacct tttcatataa ttcgtccacg ctttcaggat tgctgacgtc
     7981 taccttgaaa gcataagcct ctttgccgct caagcgtctg cactcgtctc tgacaccttc
     8041 tacaaatgcc attcttcgtg cgcaacttat aacgattgct ccttgctttg ctgcttcata
     8101 acagatttgt gcgcccagac cgcctgaact acctgttact aaaacaactt tatcttctaa
     8161 attcatgcct cttcctcctc gcgaaatgga atctcaatgc tatcaaaatc tttcataatc
     8221 gtcgtgtttt caaacacttc ttgtgcttct ttttcaagct ctaaagctgc ttttcctaaa
     8281 tatctcgcac tgatatgagt aagtagcagc cgttttgcct gcgcttcttt tgcaacttca
     8341 gcagcttgat gagtcgtgga atggaaatag ttgtgcgcca ttcttgcttc atctttatta
     8401 aatgtgcttt catgtaccag aatatcgcta ttttccgcaa gcacaacgga attatgtgtt
     8461 tttctcgtat cgcctaaaat agtcacgatt cgtccttttt tatctggacc tacgaaatct
     8521 tttccattga tcgtccgccc atcttctagt tggatcgttt caccttgctt cagtttgccg
     8581 tacagtggtc cagctggaat acctaattct tttagacgat ccacctgcaa ttctccttta
     8641 tgatcatgtt caaccacacg ataaccaaaa ctgtcgatcc catgatttag tttttttgta
     8701 taaacagaaa attgctgatc agtaaaaatc ggggctgttt catttagctc aatgaatttc
     8761 agcggataac ttaaacgcgt ttgcgaaata cctaaagaga cttttatgta ttcctctatc
     8821 ccttttggcc cgtaaatctc taatggcgta tccccacctt gaaatgaccg gctgctgatg
     8881 agcccgggaa gtccaaaaat atgatcgccg tgtaaatgtg taatgaaaat tttaccgatt
     8941 tttctcggac gaatagtcgt tcgcaaaatc tgcatttgcg tgccttcgcc acagtcgaac
     9001 aaccaaactt cattgcgctc gtctaaaagt ttcaatgcta tacttgtcac atttctatgt
     9061 tttgcaggaa cacctgcacc tgttcctaaa aattgtaatt ccataatcta cctgactttc
     9121 tttaagttcc tctttaattt tagggtaaaa agaagggata atcaaccgta agtcgagaat
     9181 gggttcaaat taaaaataac gttctaaagc gatagcgttt tctttgaaaa ttactataaa
     9241 aacagcagcg aacccgtttg agatcctctg ctgtttttta ttaatttgaa gatccagaac
     9301 tttcattgtc gttggaagtt tgcggatgtt tgtccttcca agtcttttgt gcagcttctt
     9361 tcgcatgatt ttcagcttct ttcgcatcat gcacatcttt ttcgacatct tcataatcca
     9421 agcgagtaat ttctccgcct aattccatca ttaccaattg attcagtggg cgattgtcct
     9481 cttcatcagc aatcaagatc agtcctgtag ctccttcacc gattttattt cctacaaagt
     9541 cgaacacggt ctgggcatct tttatttctt tggcatcttg tgtcgcaccg atcattcctc
     9601 cggcaaacca tcctaataaa atccctagcg gaccacctaa tacaccaacc aacattccga
     9661 ttaatccacc cgtagaagat ttgttattac cggtaaagtc gataaaatct tctattttga
     9721 attgatgtgt cccattagga tcatggctga caactgccat ctgttctcct ttaatcgaac
     9781 gaacagcatg taactttttg atttctgaaa atgcctgata tgcctggctt tctacttgaa
     9841 aatttaaaat gattactcgt ttgctcatta aaaacgcccc tttcatacct tcttgttatt
     9901 tattttacct ttaattgaaa aataagaaaa gaaaagcact ctttttttcc aataaaatag
     9961 ggctgtgaca aaagtcgtat cacagctcct ttttccgaat aaacggcgtt cttgaagctg
    10021 atcacttctg tcacaacctc ccttaaatct gaatctatat tttttggtat ttgtttttat
    10081 ttgcttgcct attcgacaaa ttcaaattca aactcaccga tacgtacaag gtcgccatct
    10141 ttcgcaccgc gtgcccgaag agcttcatcc acacccatac cacgtaattg acgagcaaaa
    10201 cgcatcactg tttcatcatg atcgaaattc gtcatttgga ataatttttc gatcttctca
    10261 ccagataaga tccacgttgc atctgaatca cgatcgattt ggaactcagg accttctgga
    10321 ttgaatccat agtgtacagt ttcttcttcc agttcttctt cgtacaatgg gaattctggc
    10381 gttacttcta acaaatctgc tgtagcattc aataacgcgt ccaatccttg tcgagtcact
    10441 ccagaaattg gaaatactgg gatgtcatca gcaaattcat cttctttttc cttgttcagc
    10501 tgttctttga atttcactag attttccggt gcatccggca tatccatttt gtttgccacg
    10561 atgatttgcg gacgctctag caagcgtaag ttataagtag ataattcttt gttgatggca
    10621 agatagtctt cataaggatc tcgtccttcc atcccactca tatcgatcac atgtaaaatt
    10681 actcgtgttc tttcgatatg acgaaggaat tgtgtaccca gcccaacacc ttgcgaagct
    10741 ccttcaatca agcctggcaa gtctgctgcg gcaaaacttc tgccatcact tgtagtaacc
    10801 attcctagat taggtacgag cgtcgtgaaa tgataagcgc cgatttttgg acgagcagat
    10861 gaaataacgg atagtaaagt cgatttcccg acagatggaa aacctacaag tccaacgtct
    10921 gccaatactt tcaattctag ttcgattttt cgctcttgtc ctggttcacc gttttcagca
    10981 atttccggtg caggattccg aggagaggca aaacggatat tccctcgtcc accgcgtcca
    11041 ccttttgcca caactagtgt ttgaccattt tcaattaaat ctcctaaaag agcaccagtc
    11101 tccgcatctc ggacagtggt tccttgtggt actttaacat aggtatcttc tgaaccacga
    11161 ccgtgcatcc ctttactcat cccattttca cctggctgcg ctttgaaatg gcgattgaac
    11221 cgaaaatcca tcaatgtacg taacccttca tcgacgatca agatgacatc gccgccacga
    11281 ccaccgtctc cgccagccgg tccgccgtca ggaacgtatt tttcgcgacg aaatgcaacc
    11341 attccgtctc cgcccttgcc agctttcaca tcgatcgtta cttgatctaa aaacatggac
    11401 ataattaagt cctcctatta ttcatttatt tatttgactg cctgaatttc cacaataaac
    11461 aaaagtctta cttattttat cggtgaaatc tgattttgtc tatcatttca gcaaaaagat
    11521 tgtttacctt cttctttttt tatcatttgg taaggtttcc aacctttttt ctgctaccaa
    11581 gtataatttt ttgacaaaat attctttgcg tagcagaata acagtgtatt aattgtatta
    11641 attgaggatg ccgtttcaca gcacccctaa taaaggagga acttttgata tgacagtaaa
    11701 agtaggtatt aacggatttg ggcgtattgg ccgtctggct ttccgccgca tcaaagaagt
    11761 ctcagatgat attgaagtag tagcaatcaa cgacttaaca agtccaacta tgttagcaca
    11821 attattgcaa tttgactcta ctcatggtac ttatcctggc acagttactg caacagaaga
    11881 ctcaatcgta gtggatggcg aagcaactcg tgtttatgca gaacccgacg caagcaaaat
    11941 cccttgggca aaagaaaacg gtgtagatat cgtactagaa tgtactggtt tttatacatc
    12001 tgaagaaaaa gcaaaagcgc atttagatgc tggtgtcaaa cgagtagtta tttctgcccc
    12061 agctggtgct atgaaaacca tcgtctataa cgtcaatgat gatacattag atgaaaacga
    12121 caaaatcatc tcagcaggtt catgtacaac aaactgttta gcacctatgg cttatttctt
    12181 aaataatgaa tttgggatcg aagttggtac aatgacaaca gttcatgcct acacttccac
    12241 acaaatgctc ttagatggac ctgtaaaagg cggtaatttg cgtgctgctc gttcagctgc
    12301 tgacaatact atcccacatt ctactggtgc tgcgaaagca atcggcttag ttatccctga
    12361 gctacaagga aaattacaag gacacgcaca acgtgttcca gtcgttgatg gttcattaac
    12421 tgaattggtt tcagtattga aaacaaaagt aactgctgat caagtaaacg aagcaatcaa
    12481 aaaacacaca gttgacaatc cttcattcgg gtatgatgac cgtcaaatcg tttctagcga
    12541 tgtcattgga acaactcaag gttcgatctt tgacccaacg caaacagaag taacaactgc
    12601 tggtgatttc caacttgtta aaactgttgc atggtatgac aatgagtatg gatttacttg
    12661 ccaaatgatt cgcttactag aaaaattcgc taacttgtaa tttgatagat caatcgataa
    12721 aagaagcctt tgactggatc cagtcaaagg cttctttctt ttcatcccct acttttaaac
    12781 tcgttcaatg aacatattgg cttaaaaatt gttggactac ttgctgatat ttttcaggat
    12841 ctgtcttgta tgcttccgca tgctcggctc cttttacaac atacttttct tttggtccct
    12901 tcgttgcacg atagacctta tctaacattt caaatggtac aaatgtatct tcgtctccat
    12961 gtataaagag cattgggcgc tggtttttct tcaattgttc cactgcattt gcttcaccga
    13021 aaaagtagcc agcacggact tttgtcacta gactcgttac ttgcataagc ggaaatgctg
    13081 gaagactaaa catatcgtct agctgataag caagctcatc atgaacggaa gaataaccgc
    13141 aatcttcaat gattgctttc acattatctg gtagtttttc tccgctagtc atcataactg
    13201 ttgctgctcc catgctgaca ccgtatagaa cgatttcttg cgattttcca ttgttttcta
    13261 gcactttgtt tatccactgt acatagtctt tacgctctgg ccatccaaat ccaatgtaat
    13321 ccccttcgct ttttccatgt cctcttgcat caggaacaag gacattgtaa cccatatcat
    13381 gaaacatctt tgcgtaattg gtcatcgttt cagcgtttcc catatagcca tgagccacaa
    13441 gagctgtttt ttcacttttc gtttcagcag gaagataaat cgctgataat ttcagaccgt
    13501 cttttgaagt gatggtccaa tcgcttcggt tttcttttga actaaaccat ttatgtgcat
    13561 ccgtttcttt ggatgtctgt gttgtaccag gtgtatcttc gtctaaaaag tctttctcag
    13621 aaggcacaac agcataatta taaaaataat tccctgcaaa cactaacgca acaatcagag
    13681 ctaaagcgac gcctaaaagg attttcatcc aaagtttcat aaatcatcca tctcatttct
    13741 cttttttcat ataaccttga attagtataa tcaaagaatc tctactatgc aatgttcatt
    13801 tttataac
//
LOCUS       DAYYGO010000073        13717 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000073 DAYYGO010000000
VERSION     DAYYGO010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.075"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(4..369)
                     /locus_tag="ACHA5T_001877"
     CDS             complement(4..369)
                     /locus_tag="ACHA5T_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFM8887442.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(516..1289)
                     /locus_tag="ACHA5T_001878"
     CDS             complement(516..1289)
                     /locus_tag="ACHA5T_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8887443.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(1463..2323)
                     /locus_tag="ACHA5T_001879"
     CDS             complement(1463..2323)
                     /locus_tag="ACHA5T_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFM8887444.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(2323..3741)
                     /locus_tag="ACHA5T_001880"
     CDS             complement(2323..3741)
                     /locus_tag="ACHA5T_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFM8887445.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(3798..4247)
                     /locus_tag="ACHA5T_001881"
     CDS             complement(3798..4247)
                     /locus_tag="ACHA5T_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8887446.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(4692..4955)
                     /locus_tag="ACHA5T_001882"
     CDS             complement(4692..4955)
                     /locus_tag="ACHA5T_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFM8887447.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(5054..6349)
                     /gene="rho"
                     /locus_tag="ACHA5T_001883"
     CDS             complement(5054..6349)
                     /gene="rho"
                     /locus_tag="ACHA5T_001883"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFM8887448.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(6346..7617)
                     /locus_tag="ACHA5T_001884"
     CDS             complement(6346..7617)
                     /locus_tag="ACHA5T_001884"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFM8887449.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(7782..8651)
                     /locus_tag="ACHA5T_001885"
     CDS             complement(7782..8651)
                     /locus_tag="ACHA5T_001885"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFM8887450.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            8754..8966
                     /locus_tag="ACHA5T_001886"
     CDS             8754..8966
                     /locus_tag="ACHA5T_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887451.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            complement(8943..10553)
                     /locus_tag="ACHA5T_001887"
     CDS             complement(8943..10553)
                     /locus_tag="ACHA5T_001887"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFM8887452.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(10775..11020)
                     /locus_tag="ACHA5T_001888"
     CDS             complement(10775..11020)
                     /locus_tag="ACHA5T_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887453.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            complement(11746..13086)
                     /gene="glnA"
                     /locus_tag="ACHA5T_001889"
     CDS             complement(11746..13086)
                     /gene="glnA"
                     /locus_tag="ACHA5T_001889"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFM8887454.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(13114..13506)
                     /locus_tag="ACHA5T_001890"
     CDS             complement(13114..13506)
                     /locus_tag="ACHA5T_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8887455.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         3872 a         3006 c         2334 g         4505 t
ORIGIN      
        1 gatttattcc attttccgtt catctggtac gatgccttga cttagcaacc acttgtggaa
       61 gaaccattct gccacaccga tcaatagagc aggaatcaat actttcatcc aaatatcgac
      121 tgtatcattc ataaaatata gatagagcca caagataact gcagctgaac cagcatctgc
      181 gatcgtcgct gcgatgttcc ctactttacg taagatcaac agatccccta gcacatagat
      241 catgactaca ataattaccc caacaatcgt tgaatccata aagctaacac cgtataaacc
      301 tgtcagcacg acccacagaa tcgcccagat catgattgcc ttcactacca acgccttcac
      361 atgtttcatt ttctgcactc ctttctcctg gataaaaaag taagattatc taacaagata
      421 accttttcat tattatacca tcggctgaga gaagcgagca aagataaggt cctatctaaa
      481 aatgaggaag ccacttagaa cattcacgta ataatttatc cgtacagaca ttcgatatgg
      541 tgttccttta ctttcgcata ctgttctttt gtcagttctt tacttgtcac cagataatcg
      601 atttcatgaa aagcaatggc ttgatgcatg aatttatgtg caattttttg ctgtgtaact
      661 aaaacggcaa gccggtctga ttgttttcta aaagcttttt tgaccattgc ctctccttca
      721 tgagtttctg ttactccttg gctgaaatct agcccgcgac aactcattat cgctaaatcc
      781 gcataatatg acaatgcatc gttataggct tttgaaccgt tgattgttgc tcgtttcgga
      841 acaattgccc ctcccaatac agatactttt gccgttgtat attcactcaa catagcagct
      901 gtgacgatgc cattcgtaat gatctgcaaa ccatcaaatc ttgctaggtt tctgactaag
      961 tgtaaagagg ttgtactgga atccaagaat aagaccatat catctcgaat caaagaagaa
     1021 gctgtatctg caatcttacg tttttctgtt tcttcttcta atttggtgat cttcatcagg
     1081 taaggcacgt caatttttcg attgtcaata agcatggcgc caccgcgaac tctacggatc
     1141 aatccacttt tttccagaaa aacaaggtca cgtttaatcg tggcttcgct ataatttact
     1201 aactcgatca aatcggtgac tcttgcaaat tggttgtttt ttagataatt cacaatctcc
     1261 tgctgtctat tctccacttc attcaacata aaatcacccc atttacttta tatagtaaca
     1321 ggaaaaaaga ggttgtgaca gaagtgattg gcttcgagaa ataagaagaa atttttcgaa
     1381 aaaactactt ctgaacatcg tgtgtggtta agagggtatg acaagactat tgtcataccc
     1441 tcttgaccac acatttttat ttttagtata aactagcctt gcctactgtg ttcaaaattt
     1501 ccattttatg tttgaccact tcttgtacct ttttattagc tgctggataa attgcattcg
     1561 gctcgtacat cgatggattt tcactcaata tttctctcaa agcattgaaa aagacgcttt
     1621 taagatctga gctcaaattc actttggcaa ctccgtattt tactgcttcg ctgacttctg
     1681 cgtccggatt acctgaaccg ccatgtaaaa caaatggaag atcgatttgt tggttcaatt
     1741 ctttcagtaa gtcgatattt aattttggag ttctgttttt cggatacaag ccatgcgctg
     1801 ttccaattgc tacagcaagt gtatcgattc ctgtttcttt cacaaaaatc ttcgcttgat
     1861 caggatctgt ataaatgatt gtttcagcac ctccttcagc agaaccatta ttcccaatcg
     1921 ttccaagctc agcctccact gagacattta ccgcatgagc taacgcaacc acttttttcg
     1981 taatctgtac attgtcgtcg aatgatttca aagacgcatc gatcatcacg gaagaatagc
     2041 cgtttcgaat cgcccgtaag acatcttcta tcgtaccgcc atgatccaag tggataacga
     2101 tcggcagttt acttgtataa gcataggctc ttactgccgc aacaaactca tcacttaaat
     2161 aggcgatttc atcaggatga atttctaaga ttactggtgc ttctaatctc tctgcttctt
     2221 cgatgattgc tttcatcata tcatagctgc aaatattgaa ggctggtact gcaaattgat
     2281 tctctttagc cactttcaat aactctttca ttgtatataa cattacgctt ccacctcatt
     2341 tgttagattt tcatttttcg tccacttgtc ttgttttttc tctttcactg tttttttcaa
     2401 tagtccaagc ataaaggctg tagtgactgt acctgatgca attgccaaga catacatagg
     2461 taaattgttc ataacatttg gaattagcat cacccaaatc cctccatgag gtgccattga
     2521 agtaactcct actgccatgg aaatccctgc agcgactgct gaacctgtca tcaaactagg
     2581 tatcacgcgg atcgggtcag ataccgcaaa cggtatagca ccttctgtaa tatacgaagc
     2641 gcctaaaatc cagcatgatt gacctgaagt ccgttcttct ttggtaaatt tatgtttgaa
     2701 taataaagtg gctaaagcaa tacctagggg aggtgtcatt cctgcagcca tacaagcagc
     2761 aatcggtgca tttactccag ctgacatcaa accgatcgag aaagtcgaag tcgatttatt
     2821 gatcggccca cccatatcag ctgccatcat gatcccgata agtaatccga acaatagccc
     2881 ggatgtattt cccaagctat tcagccaatt tgtcaaagca tccaacaccc atgtgatcgg
     2941 ttgttcaatc acaaatacca tcaaaagccc aataattaag atcgataaaa aggggacgac
     3001 aatcagctga taaatagcag aaaattcttt ttttacgcta attttatttg ccagaaaatc
     3061 acagatatac cctgctagca cgccaccaat cattccgcca agaaaacctg aaccacctgt
     3121 agaggccagt aatccggcaa caattcctgg ggcaaaacct gcttttccag caatcgatgt
     3181 agcaatcccc gcgcctaacg caggaaccat catggcaaac gctgtatctc ctccgatcct
     3241 tgaaaatgca gctgctaaca cattatagtc tgctgagttg ggatcagccg ctgtaatccc
     3301 aaaggcaaaa ctcagtgcga tgatgattcc gcctgcgatc acaaatggaa gcatgtaatt
     3361 cacaccattc atgaaatgat tatagattgt cggctttttc tcatttgcag ctggttcttt
     3421 ttcattagac acaacgaata cgccaatatc tctcaatgct tcgtctatta ctacttgcgg
     3481 ttctttgatt gctcggcttg tagagacacg ctttactttt ttcccggcaa atcggtccaa
     3541 ttcgatttct ttatctactg caagaatgat tgcatctgcc tctttgattt cttgttcaga
     3601 taactgattt tctgttttta caccttgtgt ttcgaatttt gcttgatggt ttagatttct
     3661 tgctgttact tctaattttt ctgccgacat ataagtatga gcaattccag tcgggcaagc
     3721 agttacacca acgattttca tgattatttt cctccttttt cttcactatc gatactcgga
     3781 caaagactct actattttca ttcttgacta atctgtctga tgatatctgc tggcgtagtg
     3841 gcttctttta ttccctttct aaaatctgca ttcatcaatc gtctggagag tgtgctgaga
     3901 atctctagat gcatatctgc tgatttttca ggaactacga tggcaaaaat atattcaatt
     3961 ggcatttcat ctatcccgct atagtccgtt aatttggctg tatgaaaaaa acacagtgct
     4021 ggttttgtca catatttgct ttttgcatgg ggaatcccaa atccatcttc tattcctgtg
     4081 gagatctcct cctctcgttt gaatagtgcc ttaacgactt tttgaacatt tcttacctta
     4141 tttgtttttt tcaacacatc ccctattcgc tcaaataatg atccttttga atctatcgat
     4201 ggatcgaaaa caatcagttc ttctgcgatc aattgatcaa acctcataaa aaacctctcc
     4261 cccacaaaaa atgttaacgt tttcttggaa ctgaggtcag tatagcatcg ctagctgtac
     4321 cgttcaatat tatgaatgaa cagaggaaaa agacccatca tccgcgtgta taagtcatta
     4381 aaatgaacga tctagctcac aaacaatcgc aagtctataa caaaacaaaa agccttcctt
     4441 gaaaggaaag cttagcttct aattttaggt aaatggtttt ttacttcgaa ataacccctc
     4501 ttcatttcgt taatataagg tgtttttatc aagtggataa ttaaaactat atactagttt
     4561 ttcattgttt tgtgcattat gatcctacga ctttttcagc atcaaacgaa gttttaaatt
     4621 ggcaaaaaac ataaaaaaac agactatccc tatcagatag tctgctaaac gttgagttat
     4681 cagcgttttt attattccgc agcgtttgcg tctttgagac cgtatttttt gttgaaacgg
     4741 tccacacgtc cgtctgcttg tgtgaatttt tgacgtccag tgtagaatgg atgtgagtca
     4801 gaagttactt cgacacggat gactgggtat gtattaccgt cttcccattc aactgtttct
     4861 tgtgaagttt ttgttgaacc tgacaagaat ttgaaaccag ttgttgaatc catgaatact
     4921 actgggtggt aatctggatg gatgttttct ttcatgattt tttcgctcct ttgccctgct
     4981 ccatttgctg aaccagagtt gatttgtcga tttacaacat taacatctta acatgtttca
     5041 gtcaaggttg caattatctt ttcagatttc tggtcggatt ttttttacca aaggagacat
     5101 cttgaaattc tttgaaaaat tgctgattgt tttttgtttt acgcaaaaag cggatcagtt
     5161 gttccgtata ttctaatgaa tctccagtca tattttttcg aagtttccaa atttcttcta
     5221 attcttcact tggcatcaat aattcttctt ttcgggtacc tgattttttg atatcgatgg
     5281 ctgggaagat ccgacgttca gataattcac gtgaaagttg taattctaga tttccagttc
     5341 ctttgaattc ttcatagatc acgtcatcca tacggcttcc tgtatcgact aaagcggtcg
     5401 ctaaaatcgt caaactgcca ccttcttcga tgtttcttgc tgccccgaaa aatttcttag
     5461 gtttatacaa tgcggctggg tcaatcccgc cactcaatgt acgtccgctt ggtggaacga
     5521 ctaagttata ggcccgagca agtctagtaa tactatccat caagataacg acatctcgtt
     5581 tatcttcgac taaacgcatt gcgcgttcca atactaattc agaaactcga gtatggtttt
     5641 gcggctgcag atcaaatgta gaagatacca cgtcgccttt cacgcttcgt tctagatccg
     5701 ttacttcttc cggtcgttca tcaatcagta gaacgattaa ttctacttcc ggatagtttt
     5761 cggtgatacc atttgcgatt tcttttaata cagaagtttt ccctgctttt ggcggtgcga
     5821 cgatcaatcc acgctgacca aaaccgattg gagaaaagat atcgatcatt cgggtagata
     5881 gtctttgcgg tgttgtttcc agttgaagct gtctttgcgg ataaagaggt gttaaggctg
     5941 gaaaatgcgg acgttctttt gcttcttccg gatcttttcc attaacgctt tccacgtgca
     6001 tcaaaccata ataacgttcc gattctttag gtggacgggc ttttcctgcc actttgtccc
     6061 catttctcaa actgaatcgt ctgatttgtg aggcagaaat atagatatct tctgcacttg
     6121 gtccataatt gattggccgt aaaaaaccgt aaccatcttg accaacgata tccaatattc
     6181 cttccatata ataaaatcct tgtttctctg cttgtgcacg aatcacagct agagataatt
     6241 cctttttatt catcttactg taataaggaa ttttaaattc tttggcataa ctataaactt
     6301 cttttaacgt tttgttctct agttctgcca ttgtcaaata atcgctcatt cagctcccac
     6361 ttcctctatc atttcaattg atgcccctaa ggctgttagt ttctcgataa tatgatcata
     6421 accgcgtaag atataatcta cattgtaaat cgtcgttgtc ccttctgcca atagtccagc
     6481 aatcactaaa caagcacctg cccgaagatc agaagccacg acttctgtgc cgtgaagttt
     6541 atttggacca ttcacgatga tcatatttcc ttccacggta atatcagcgc ccattcgtgc
     6601 tagttccggc acatggttca cacgtttcgc atagatcgta tcgatgattt ctgaagtgcc
     6661 tgtagtcatc aatagtaacg cagtaagtgg ctgctgcaga tctgtggcaa atcctggata
     6721 aggataggtc atgatgtttg cgggttttaa ttctttcgag gggtaaactt cgatttcatc
     6781 ttcgctgatt ttcatatgga caccaatttc ctgcaatttt gctataaagc tttccaaatg
     6841 ttcaaagatc acatttttca ctttgatacc atttcctact gcagctgcta atgctagata
     6901 ggttcctgct tcgatgcggt caggaatcat gaaatgccga cagccatgta atgtttcgac
     6961 tccttcgata cggatcacat ctgtacccgc tcctcgaact tttgccccca tattattcag
     7021 taaagttgcc acatctatga tttccggttc tcgtgcagcg ttttcaatta ctgtctgtcc
     7081 ttttgctttt acagcagcta acatcacatt gatcgttgct ccgactgaaa ccacgtccat
     7141 gaaaatccga ttgccacgca atcctttatt ttctgtccgt aaatacatgg caccatgttc
     7201 atttgtcact tcagcaccta gagcttcaaa tcctttgacg tgtaggtcaa tcggtctagg
     7261 gcctaaataa cagccacctg gcaggcctac taccgcttcg ccaaatttac caagcagtgt
     7321 tcccataaaa taataagaag cacgcaaact attgattttt ccgtgcggca tcggaatcga
     7381 tacgacttct cgaggatcga tttctagtat gttgttggaa aaatggacag ttgcgcccat
     7441 gatctctaaa atttcaatta atgaatgaac atcttgtatg tccggcacac cttccaagat
     7501 cactggggaa tcagctaaga ttgcagctgg aatcaatgcg actgcactat tttttgcacc
     7561 gctgatcgtt acttcaccag ctaaaggtcg gtttcctttg attacgattt ttttcatctg
     7621 tttccacctt gtttattaat cttttttcat ggatatgctt gtattttatc atagataaaa
     7681 aaaagaaacg agcatggtct gccgttttaa gtaaaaaaga atggagagcg acttcaaaag
     7741 tcgttctcca ttcctatata tcgtatctgt tatgtggatg cttacgcttt gttagctgaa
     7801 ccgaaccagt cgatatgaga tttagcatct tcaacgattg ctgctttacc tggagctaat
     7861 aatttacgag ggtcgaatcc tttaccttct ttatctttac cagcttcgat atatttacga
     7921 gtagctgcag caaatgataa ttggaattca gtgttaacat ttacttttga gatacccatt
     7981 gagatagctt tttcgatttg atcttgagga atacctgatc cgccatgtaa tactaaaggc
     8041 atgtctggac ctacagcatc agcaattgct tgtaagtggt caaaagctaa tcctttccag
     8101 ttttctggat attgaccatg gatgttaccg ataccgcaag ctaagtagtc gatacctgta
     8161 gcaaccattt gtttgcattc ttcaatatca gctaattcgc cgttaccgat gattccgtct
     8221 tcttctccgc cgattgaacc aacttcacat tcaactgaga taccttttgc atgtgctttt
     8281 tcaacaacgt ctttagctaa tttcaagttt tcttcaaatg gtagatgtga accatcgaac
     8341 attactgaag tatagcctaa ttcgatacat tccaaagcag cttcgtaatc accatggtct
     8401 aagtggatag caactggaac agtgatgttc attgcttcaa ctaaatcagt gattaaatct
     8461 ttacagattt tgtaaccacc catgtattta gctgcaccca ttgatgtttg gataagtact
     8521 ggtgcttgtt ttgcttcagc cgcttcaagg attgcttgag tccattccaa gttgtttgtg
     8581 ttgaatccgc cgacagcgta accgccttta cgagcagctt ttaaaaattc agctcctgat
     8641 actactggca taataaaatt cctcctaagt tttgtaaaac taaatttgtt aagacaattc
     8701 ttaaccaacg accctatttt accaaacatt tcggtagttt tctactaaaa tcaatgaaaa
     8761 tgaaaatttt caagaagttt tttgcaaaaa ataaaatatc caatagcgct ttaatcgaaa
     8821 aaagtctttt gctttctatc ttttttccta caaataataa caaatcagaa aaagagaaaa
     8881 gtgacaagaa aattgcttat ccccttctct tgctactttt ctctttctat caagttcaaa
     8941 aattattttg tttcgtgatc tgctaatgat gctccgacaa atgctttgat taatttttgc
     9001 gggcgatttg gacgagaaat caattctggg tggaattggc atcccacaaa gaattttttc
     9061 tctggcagct ctacgatttc tactaaacgg ttatctggtg agacacctga aaagactagc
     9121 ccttgttctt caaacaattg acgatatttg ttattgaact catagcggtg acggtgacgt
     9181 tcttgcacaa catcggctcc atcatatgct tcagccgtct tcgttccttt tttgatttta
     9241 catgggtata atccaaggcg taaagttcca cccaaattct cgatgttttc ctgatcagcc
     9301 attaaatcaa taatattgtt tgctgtatct ggattcgttt cagctgatga cgcatcttct
     9361 aatccaacga cgttacgtgc aaattcgaca caagccatct gcataccaag acaaatacct
     9421 aaaaacggaa cgtcattttc gcgagcataa cgaatcgctt cgattttccc ttctactccg
     9481 cgatcaccaa atccaccagg aaccaagatt ccatctgcgt ctttcaaacg ttcttctaca
     9541 ttttctctcg ttaattcttg tgctttgatc caatcgatct cgatgtctga attataggcg
     9601 aaaccagaat gtttcaatgc ttcaactact gagatatatg catctggtaa ctcgacatat
     9661 ttaccgacaa gcgcaatccg tacttttttc ttcaagttca agacacgttc ttctaattct
     9721 ttccattctg tcatgtctgc ttctggtgta tctagtttta aatggtcgca aacaatttga
     9781 tccattcctt gtgcttgtaa aactaatggg atggagtaaa gtgtatctac atctggcgac
     9841 tcgataactg cttctggttc gacatcacaa aattgtgcca atttgttttt cacgctttga
     9901 gaaacaggtt gctctgtacg taccaccaaa atattcggtt ggatacctaa accacgtagc
     9961 tcttttacac tgtgctgcgt tggtttagtt ttcatttctc cagcagcttt caagtaagga
    10021 atcaatgttg tatggatata cataacatta tctgtaccga catctgcttt catttgacga
    10081 agtgcttcaa ggaatggcaa agattctata tcccctactg ttccccctac ttctgtaatg
    10141 atcacatctg aatcagtcat tttagctgct cgcatgattt tttctttgat ctcatttgta
    10201 atatgaggaa taacttgaac agttgctccc aagtattcgc ccttacgttc tttacgcaaa
    10261 acttcagaat agatttttcc tgtcgtcacg ttggagtatt tgttcaaatt gatgtcaata
    10321 aaacgttcgt agtggcccag atccaaatct gtttcagcac catcatctgt tacgaatact
    10381 tctccgtgct gatatgggct catcgtcccc ggatccacgt tgatgtatgg atcaaatttt
    10441 tggatagtta cttttaagcc gcggtttttc aataagcgtc ctaaagaagc tgcaacaatc
    10501 ccttttccga ttgatgaaac gacaccgccg gtcacaaaaa tatattttgt cataagttca
    10561 aaaactcctt tttaatttat gtgcaggaaa agtctcagac aataaacaaa gctccctatt
    10621 cttcaataag aatagggagc cgaaatttct gtaaacttct gaccctctaa aagggtgccc
    10681 aaggtgtata atacaagctg actaagggaa agtcaagtac tcaaatgaat actatccgct
    10741 tacaattttt tctttttcga tatcctttga gaaattattt cttaagtcct ttatgagtgc
    10801 aaaaaaaatt taacccacat aataagcttg atataaacat agctaacgtt aaacctaagc
    10861 aaatcagaaa atcgttactg aaaaatttta agtgaaataa tagataaaaa aataaaacaa
    10921 acattatgat agataatatc ccacctaaca aagaactact ttttcctact tgcagcaaca
    10981 gtttatatag cttatcgtag aaacaatctt tttctttcac ccttatttac ctcctttacg
    11041 taaaaaacgt agtccacagt tctcggattt ttggcatatc ttttctatta aataatatca
    11101 aaaacaaaac aattccacta attttttcaa agatgacctt tttttcaaca aaagtgcctt
    11161 gtagggattc attgaaggta acgaatcaca taagtaaaaa ataaggagat gcgacaaaag
    11221 tcgtgcctcc ttatttctaa ataaacggtg ttctcctgcc aactcctcgg tcacaagtca
    11281 caatatccat caaacatgag aagctgtttt tgaaattatt tcttgtgatt cggagttaaa
    11341 tggtaggctc acaacctctg gataaacggt attcaaaagc cagtttctcg gtattaagcc
    11401 aaatttatgc aaaaatgtag aaagccattt tcacaaaatt tttcttaata ctcgaaacta
    11461 aacgtctttt tcaaaacctc ttggtaaacg gtgttcaaaa gcttgcatct gcggtaataa
    11521 gttcagccaa ttcaaaaaca agaaaagctg tttttgcttg gctattctta ttacttgttg
    11581 cagggcagct ttttcacaac ctctccacta aacggtgttc aatcagctga ccctcggtat
    11641 tagctcaaaa aacggcaaaa tatgaggaac tatttttccg ttagtttctc taatactcag
    11701 gtcaaaacgc tgatttcaca gcctctaatt cacggtattc ctgttttaat aaagttctag
    11761 atattgttct ctttcccact cggaaacagt ttgtctgaat gccgcccatt cgagacgttt
    11821 tgcttcaaca aaatttgaga agatatgttg tcctaatgcg tcaatcatta cattatcctt
    11881 acgcaattct ttgatagcat tatggatagt tgaaggtaag tcctggatat gtgcagcttc
    11941 tctttcttcc tcatccatta cataaatgtt acggtcaaca gctgctggcg gtgtgatctg
    12001 attacggata ccgtctagtc ctgcttgcaa caatactgcc attgccaagt atgggtttgc
    12061 agatggatca actgaccgca attccaaacg tgtggataaa ccgcgagatt ctggtacacg
    12121 tactaatggc gaacgattac ggccactcca tgcgacataa acaggcgctt cataacctgg
    12181 aacgagacgt ttataagaat tgactgttgg attgcaaacg gctgtgtagg cacgagcatg
    12241 tttcagcaaa ccacctaaga aatgataagc tgtttcactt agttccaatt caccgtcttt
    12301 atcatagaaa acatttcctt catcattgaa caaagacata ttgcaatgca taccagaacc
    12361 attgatccca aataacggtt tcggcatgaa agtcgcgtga agtccatgct tgcgtgcaat
    12421 cgttttgaca acgagcttga aggtttggat attatcacaa gcatctacca catctgcata
    12481 tttaaagtca atttcatgct gtcctggcgc tacttcatga tgtgaagctt ccacttcaaa
    12541 gcctagactt tctaattcca acacgatgtc acggcggcaa ttttcaccta aatctgttgg
    12601 tgcaaaatca aaataaccac ctttatcatt caaggtcgtt gtgatcccac cgtcttcgtc
    12661 taatttaaat aagaagaatt ctggttcagg tcctaaattg aaagaggtaa aaccaaggtc
    12721 gcgcatatct ttcaatgctc gtttcaaatt cccgcgagga tcgccggcaa atggtgtccc
    12781 atccggatta tagatatcac aaattaaacg agcaactttt ccatgagcac tttcccacgg
    12841 gaagatcatc caagtggaga gatctggata caaatacata tcgctttctt caattcgaac
    12901 aaatccttca atcgaagaac cgtcaaacat cattttattt tctaatactt tgtcgatctg
    12961 gctcactgga acttctacat ttttgatcgt acctaagatg tctgtgaaca tcaatcgtaa
    13021 aaatcgtacg ttttcttcct caatgattcg tttgatttct tcacctgtta tctgtttctt
    13081 taccatggta tccctcttcc ttttttaatt tattcagtca aagaattgtc tttgcaatcc
    13141 ttgcgactga taatgacctg ttttgcttaa cccaccttgc gccaaaatct catcacgaaa
    13201 aatttgtcga acgtcatgat ctgtcaacgg cttcttgttt tttgcttctt gttttttcgc
    13261 ctgtgccatt tcgtatacac gtttgatccc agccatattc aatccctctg aaagatagtc
    13321 tttaatctca agcaacatat caatatcatt cagcgaatac atacgtcggt ttccttcgct
    13381 tcgttccgga tgaactaaat cttgttcttc ataatagcga atttgccgag cagacaaatc
    13441 agtcagcttc ataactgtac caattggaaa tacggacata gatcggcgca attccttttc
    13501 tcccatcaga cgtccccttt cgttttataa gttggctaaa gtgtaccaat taaaaaaaat
    13561 attgtcaact gcatatgtta ggttttctaa catgagatct ctatttttta taaaataaca
    13621 aaaaaataag aacacaaaaa aggtatctag ttgattttcg taccagatac cttttatttt
    13681 ttattatact gtactatcct ttattagtgt ttttatt
//
LOCUS       DAYYGO010000074        13540 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000074 DAYYGO010000000
VERSION     DAYYGO010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.076"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1366
                     /locus_tag="ACHA5T_001891"
     CDS             <1..1366
                     /locus_tag="ACHA5T_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFM8887456.1"
                     /translation="VNKEVNLEKLSNLLHVCKTTLLNDINNIKKEFEEQIAYTHREKD
                     RYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDEPDYLQLTECEFISVSKAYSLK
                     KQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRLGGFNSWELPESFFERADRFIE
                     SVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTIDSKFIEEIKKRPIYNYVESAWE
                     NTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDFQQLHQVFIDNTPEIKELVATF
                     ESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKFYLLNEEQTNLYKEVQTIFSSW
                     SSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYIVTNSDVHYLIYREALEAVTTF
                     DFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDAVQYENIIPISINTIDRAIISA
                     VQNK"
     gene            complement(1458..1823)
                     /locus_tag="ACHA5T_001892"
     CDS             complement(1458..1823)
                     /locus_tag="ACHA5T_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFM8887457.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1820..2185)
                     /locus_tag="ACHA5T_001893"
     CDS             complement(1820..2185)
                     /locus_tag="ACHA5T_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFM8887458.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2377..2625)
                     /locus_tag="ACHA5T_001894"
     CDS             complement(2377..2625)
                     /locus_tag="ACHA5T_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887459.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2624..2752
                     /locus_tag="ACHA5T_001895"
     CDS             2624..2752
                     /locus_tag="ACHA5T_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFM8887460.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2809..4503
                     /locus_tag="ACHA5T_001896"
     CDS             2809..4503
                     /locus_tag="ACHA5T_001896"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFM8887461.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4603..4899
                     /locus_tag="ACHA5T_001897"
     CDS             4603..4899
                     /locus_tag="ACHA5T_001897"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887462.1"
                     /translation="MQAFEHRLPRGCEKAALHQVIRTPKLKLLLMFLTWLDLLPQMQA
                     FEHHLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQAFEHRLPRGYEKGV"
     gene            5277..6410
                     /locus_tag="ACHA5T_001898"
     CDS             5277..6410
                     /locus_tag="ACHA5T_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFM8887463.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6441..6761
                     /locus_tag="ACHA5T_001899"
     CDS             6441..6761
                     /locus_tag="ACHA5T_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFM8887464.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6790..7254)
                     /locus_tag="ACHA5T_001900"
     CDS             complement(6790..7254)
                     /locus_tag="ACHA5T_001900"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8887465.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7385..8638)
                     /locus_tag="ACHA5T_001901"
     CDS             complement(7385..8638)
                     /locus_tag="ACHA5T_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8887466.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8667..8984)
                     /locus_tag="ACHA5T_001902"
     CDS             complement(8667..8984)
                     /locus_tag="ACHA5T_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM8887467.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9010..9330)
                     /locus_tag="ACHA5T_001903"
     CDS             complement(9010..9330)
                     /locus_tag="ACHA5T_001903"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8887468.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9794..11209)
                     /locus_tag="ACHA5T_001904"
     CDS             complement(9794..11209)
                     /locus_tag="ACHA5T_001904"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8887469.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11226..12542)
                     /locus_tag="ACHA5T_001905"
     CDS             complement(11226..12542)
                     /locus_tag="ACHA5T_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8887470.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12692..13390)
                     /locus_tag="ACHA5T_001906"
     CDS             complement(12692..13390)
                     /locus_tag="ACHA5T_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFM8887471.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
BASE COUNT         4290 a         2763 c         2331 g         4156 t
ORIGIN      
        1 tgtaaataaa gaagtcaatc tagaaaagct ctccaatctc ctgcatgttt gcaaaaccac
       61 tttactcaat gatatcaata acataaaaaa agaatttgaa gaacagatcg cttatacaca
      121 tcgggaaaag gaccgttaca ctttatattt ttcagaacat atacctcgat gcaagatcat
      181 gcagcaattg tctcaaaatt cgttgttttt aaaaacttgt ctgctttatt tggaagaaga
      241 tgaacctgat tatcttcaat taactgaatg tgaattcatc tctgtcagca aagcttattc
      301 attaaaaaaa caagtattag cttattttaa tgactgcggg atcgaaatcg accgctactc
      361 ccctcgattc actgaaatgg aaagacgtct gctgcttctt aatgtgtcgt atcgtttagg
      421 cggttttaat tcttgggagc tacctgaatc gttttttgaa cgtgccgatc gtttcattga
      481 atccgtaacg gaaaatagtg gcagatttta tgataaagaa aataaagaaa tcttatcgat
      541 tggttttgct atcagtttcc tacgccagca agtgtgtgct gtgacaatag attcaaaatt
      601 tatcgaagaa atcaaaaaac gtcctattta caattatgtc gaatcagcat gggaaaacac
      661 tgatttccaa acctattata aaaaagaaga attcgccttt attttgacac tattcaatct
      721 ttgtaattat ggtttccatt cttatcagtt gattgcggaa gactttcaac agctacacca
      781 agtgtttatt gacaatactc ccgaaataaa agaactagtt gctacctttg aatctcactt
      841 caatcaagaa ttattcggaa accaaccgtt cgaacgtgcg ctgattcatc tgatgcgttc
      901 ggcttgggat aactatcaat tattcatgcc agaaaaattt tatcttttaa atgaggaaca
      961 gacaaattta tataaagaag tccagacaat tttttccagc tggtccagcc agttacctta
     1021 tgatctacgg ttgaatccta attgcatgcg ggcttttgtg atcgaattga gcggaattct
     1081 ccgtctgaca aaagaacatc tgactatcta tatcgtcaca aattcggatg ttcattatct
     1141 gatttatcgg gaagccttgg aagctgtaac tactttcgat tttcaagttg caccaacaat
     1201 ttattcaagt atatctgata taaagaagta tgcgcagcag tcctcaaacc gggtattatg
     1261 tgaacgtaca ttatatacac cagatgcggt ccaatatgaa aatattattc ctatttcgat
     1321 caatacgatc gatcgagcta tcatttcagc tgtgcaaaac aaataagggg ctgtgacaaa
     1381 aatcatgtca cagcctctta tttgttgaag atgcccattt ctgtaacaat ctcttgatac
     1441 acgtcaggct cacaacctca cttattttgg acatctacgg gtccgatctc tttatttaaa
     1501 aaataagaaa tgatggttcg gataactacc aacgccgcta gttggaaaat atcctgcaag
     1561 gtaggtttga taatcgattc gatgatccca gctgaaacaa gaatttccaa acttagaagt
     1621 atatagcctc ccaacatctt tttaatagca ttgttctcgc tgactcggtc gctttgcact
     1681 tttgtatgga caaataaata tttcaaaaaa tccttgattg atagacaaac accccaaacc
     1741 aataaacaga tagcaaagat atcaagtccc agtacaagtt tttcgaaata tggaagaaga
     1801 aactgcatca actctgtcgt catcctacct ctcctcttct ttgtttttct ttgtcttcta
     1861 tttcctgatt caagaaataa gaaatgactg ttcgaatgat aataataagt gcaagcttca
     1921 aaatatctgt ccaagtcggc ttgataactg actcgatgat atcagcaacg actagtactt
     1981 ccaagcttaa caaaatataa cttgctagca gttttcttgt atctgcatta cgagtattac
     2041 gttctgcata gtttttcttg acatgagacc aatcaaaaag tttgaataga acaagaacga
     2101 tcccgacaat taagataccg atagaacaaa tatttaaaat agcaatgatc cattctagaa
     2161 attcaattaa atttgagcga aacatatacg ctacctactt tcttttaatt tgttcctatt
     2221 cttttattgt aaaagaaagt taccttgttc gtctatttaa aagttgttct aatgataacg
     2281 gttcgcttaa atttttcttt ttcactctcc catttttctc cctccatcca taaatcgtgg
     2341 aagtatccaa aacaacgtag caagttaaaa taaggcttag tccgtcatca gcttttcaaa
     2401 gaagcactgt ctcggtgctt tttttgtacc caaatttcta aaaatagaac catacacctc
     2461 ctcaaatctt ttcttcttac ctaccctatt tttatttaca ggaaaatcag acacttcttc
     2521 ttttttgtca aaatttcctt tttcttctcg tcctatcatt tggcattcta ataaaaacga
     2581 actatccttg gatatagaaa gagaaaacaa ggaggcgttt ttcatgagta aaaaattaat
     2641 caaagtcggc atcggtttag gtttgcttgc ccttggcgct gcatatctag gaaaaaagac
     2701 aggactattc gaagatgaca gtcatttata tgatgaattc gaatcaatct agcatcctat
     2761 ttagttgaat cactacggac atacttggaa gacattggag gaatcacaat gagaaaaagt
     2821 aaagcaataa tgattggtgc cggtattgcc aatatggcag cggctgttta tttgattcaa
     2881 gaaggcaaat ggaaagggga tcagatcacc ttttattcat tggatgatca cggttcaaat
     2941 gatggtgcac cgaccgacac tgtagtggat gaatattgga ataaaaacca tccattagaa
     3001 aaccaaaaag gctatattgc tcgaggcggt cgcatgctta attaccgtac ctatgtagat
     3061 ttgatggatc tgttaagtcg aataccttct gttactgagc cagggatgac tgctgaagaa
     3121 gatacgagag attttgatgc aaaacaccga acctttgaca aagcacgttt gatggaaggt
     3181 ggcaaaggaa tcattgatgg cggaaaactg ggattcaata ataaagaccg tattcttctg
     3241 actaaactga ttgctatgcc tgattcagaa gaagaaaaac tagacaatat cactatcgaa
     3301 gaatatttca aagatgatcc acactttttt gaaacaaact tctggtttat gtgggaaaca
     3361 acatttgctt tcagaacacg tagttctgct caagaattgc gtcgctatat gcatcaaatg
     3421 atttatgaat tcactcaaat cgaacatcta gtgggcgtca atcgaacacg ttacaatcaa
     3481 tacgaaagta ttatgcttcc tttgatcgag tacttgaaag aacaaggatg caaaatcatt
     3541 ttgaaccgcc gtgtcactga ctgggaattc aaagaaacac cgatgcaaga tgaaatcact
     3601 gtgactggtt tgaaaattac caatacactg actcaagaag aagaatacgt tgtggtcgat
     3661 gaagatacag cagttatttt cactaatggg tccattacag attcagctac tttaggagat
     3721 tacgaaacac cagctgtaga aaatatggat tatggtgcag cttctagtct ctggaaaaaa
     3781 gccagtgaac gtttctacaa cttgggaaat cctgacaaat tctttgctga tcgagatgca
     3841 agcgaatggg tcagctttac cctgacgacg aaaaaccatt tgttgttgaa tgaaatcacc
     3901 cgtatcacga ctcaagaacc aggaaatgcc ttgaattcat ttatttcaac cacaccgatc
     3961 acgccgttag gccaaaaaga tgtcaatatg tctatcgtcg tgcatcatca accgcatttt
     4021 acgtctcaga aaccaaatga aacagttatt tggggttatt tcctttatcc aagacgacgt
     4081 ggcgaatttg tggataaaca gtatattaaa atgaatggaa aagaaatgct agaagaactg
     4141 attggacaac tttcaaaagt agatcctgga ccagtgaata ttcgtgaaaa agaaacagaa
     4201 attttcgaca gcgtaataaa taatatccct gtctatatgc cttatgcttc cgcgttgttc
     4261 aataatcgtg ccaaaagcga tcgaccaaaa gtcattccta aacattcgac caatctggcc
     4321 tttacaggag aatttgtcga gcagccttat cagatgatct ttactgaaca aagtgctgta
     4381 cgttctggag aaatcgctgc tttccatttt gcgggtattc ctatgtcccg tcttgtcaaa
     4441 acaccgcgtt acgacaaaga tattcctact ctagctcgtg ccgcaaaaaa aatgtttgaa
     4501 taatcaagag gttgtgaaaa agctgccctg catcaagtaa taagaacaac caggctaaaa
     4561 cagcttctta tgtttttgat ttggttggac ttattaccgc agatgcaagc ttttgaacac
     4621 cgtttaccaa gaggttgtga aaaagctgcc ctgcatcaag taataagaac acccaagcta
     4681 aaactgcttc ttatgttttt gacttggttg gacttattac cgcagatgca agcttttgaa
     4741 caccatttac caagaggtta tgaaaaagct gccctgcatc aagtaataag agcagccaag
     4801 ctaaaacagc ttcttatgtt tttgacttgg ttagacttat taccgcagat gcaagctttt
     4861 gaacaccgtt taccaagagg ttatgaaaaa ggcgtttagt ttcgagtatg aagaaaaatt
     4921 ttgtgaaaat ggttcctcac gtttttgcat aaatttgact taataccgag aaactgatcc
     4981 cttctgtcat aacctctttt tatttatgtc tgtttctttt tgaaaacaac tattttttca
     5041 acgtttactc tcttgccaat ccaacatcat ctaacggata caactatttc aatgattacc
     5101 attacactaa aaaatcggaa acacacctct ccgaacacaa atacataaga ctatagaatg
     5161 tttcatttaa gaaaaaaagc gtttcattat caaaaaggta agatctcctt taaaatgaaa
     5221 ttatctgtag tactaataat gcagacaatt ttatgaatct gaaaggagca tctccaatga
     5281 aagcagtcac ttggcaagga aaacagaaaa tggaaatcag aaacgtagat gatccaaaaa
     5341 tattagaacc aacagatgca atcatccgta tcacagcaac agccatttgc ggctccgatc
     5401 tgcatcttta ccatcatggt gaagcagttt tggaaaaagg gtatgttgta ggacacgaac
     5461 caatgggaat cgttgaagaa gtgggatctc aagttcaaac attgaaaaaa gggcaacgtg
     5521 tggttatccc ttttaatatt agttgtggtc attgtcattt ctgcctgaat cacatggaaa
     5581 gccaatgtga tgaatcaaat ccagatcagt tatatggcgg gcttttcgga tttggcaagt
     5641 tgaacggtaa tcattggggt ggtcaagcgg aatatctaag agttccttat gcagactcta
     5701 cttcatttgt tgtaccagat aatgatcttc ctgatgaaaa agttttattt ctatcagata
     5761 ttttgcccac agcttattgg agcgttgaaa acgcaggagt aaaaaaaggc gatacagttg
     5821 tgattctagg ttctggtcca gtcggtctat tcgctcaaaa attcgctgtt atggctggag
     5881 cagagcgcgt aatcgcagta gatccagtac agcaccgtct agataaagcg gctaattaca
     5941 atggggttga aacttatttc ttagaagaca cggcacaagc tggaacagaa ttgtatgaac
     6001 tggctaaagg tggcgcagat gtcattatcg attgtgtagg aatggacgga ttagagcctg
     6061 tcaaagaaaa agcgaaaaat ttagtaagct tacaaagcgg cacaatctct ccattacaga
     6121 tggcatcgca agctgtcaaa aaatttggaa cagtccaagt aactggtgtt tatatgactc
     6181 cagcttcttc ttacccgctg caagaatttt ttatgcggga tatcgctgtc aagcatggac
     6241 aggcaccaat tatccatcta atgccaaaaa tttacgcaat gattgcagaa ggactattcg
     6301 atcctacaca aatcattact cattcgctgc cactatcaga agcagcacaa gcatatgaga
     6361 tctttgacaa aaaagaagat aaaaatataa aagttgtttt gaaaccttga attcagtaaa
     6421 aaatacaggt gattttcttt atgcccaaaa tcaaacgaaa tacccatgag atcgaaatag
     6481 gaaaagaaga agatatcgaa atacgaggac gtcgcttccg cttctatttc caaaatcgtt
     6541 acacattgat ctcactggct gtcgatttat taaccgggat tttctatatt tttggaagcc
     6601 ttgcttcctt aactaaaatc cccgatcgat atggcaccta tttttatttg gctggtgcta
     6661 ttttcttgac gattcgtcct attctcagaa tcgtaaggaa catttttatt tacgacgaaa
     6721 aaaaagcaat gaagagaaca agcaaaaaaa aagaggcatg agaatttttc aaatctcatg
     6781 cctctttttt caccctatcg tatcactttg atacagacga gcggtcgtcc cactttcttc
     6841 cttgaccatt gtcaaaaatg tccactcgta tcgttcataa aacgtggtat gatctgtaat
     6901 taagtaaagt ttttcgtacc caagttgatg catttctttt ctgatttccg ctaatatccg
     6961 cttggctatc ccctgcttac gcttttccgg ttcaacaaac agggcacaaa gatttggtgt
     7021 cagatcaggg cggttatgga agtcgttttc aatgacgccc gctcctccga cgatggtttc
     7081 tttttcatct aagaccacat accattgagg aatactttcc ttattagcga ttccagtacg
     7141 aatactttcc tcatacacaa cagcaggaac tccccatttt gatgaaaacc aattcgcgct
     7201 ttgttttact aattctgggt gttctcttaa tttgatccag ttatatgtaa ccaatgttta
     7261 aacctccgac taaaataaaa cgtttacaaa aattatacaa gtctttatga aaattagcaa
     7321 tgacaacaat agtgatgtaa aaaaaaaacg gaaaacaatc acttgttctc cgttttttga
     7381 ttaatcaagc atctaaagct tcttttcttc tttcttggat cgtagctacc ttgacgaacg
     7441 gtagatagat caaaatagaa agaaccaaat tgacagcggc tagtacacca cctgcaatac
     7501 tttttgttgc caatacaccg ccaataattg gtggagttac ccatggaggc ataacggtag
     7561 ctgctggcac taaacctgta gaagtcgcaa agtaagcaac actaactaag accattggtg
     7621 tcaaaataaa tggaatgaac atgatcgggt tcaacacgat aggtaaccca aacatcgttg
     7681 gttcattaat attgaagaca cctggcgcaa tcgacaagtt tgttacaacc atatatggtt
     7741 tgtttttacg tcctactaaa taaatagcta acagtaaacc taaagttgca cctgttcctc
     7801 ctaagttgac aaagctgtca aagaaaggtt tgttcacgat aaacggtgcg ctatgcccag
     7861 ctgcgattgc attaacgttt gcttcaatgg ctggcgcatt gattgtttgc atcaaaggat
     7921 cgaccatatt cgctccgtgt aaaccaaaga accataagaa tggtgtgata aatgcaagca
     7981 gtaaggcaga tggataagag tttgcaagtc ccataaatgg ttcttggacc actgtataga
     8041 aagaacctac gatatcatca acaccgaatg cagcaaaaat tgcagcaact aaagcaaacg
     8101 cagagatagt gatcattgct ggtagtaatg aagcaaatgc ttttgcaact gccggtggca
     8161 caccatcagg cattttgatg atcaattttt catttccgct taaacgtaca aagatttctg
     8221 ttgaaataat cccaacaata atagcaataa acaaaccgtt tgagctcata cctaatgctc
     8281 cgcctaaagc gaagaaagag cctaaagaaa tcacacctgc ggaaacaccg tcttttcggt
     8341 agccgttagc taaatgataa gcaagcaaga aagcaatcaa gacagaaaga atggcaaatg
     8401 taccgttcca agcagcagcc ccgaatcctt tccatgattc accaccaaaa attgagttca
     8461 tcaattcttg gaacccggga atcggtaagt tattgatcat tactgctaat gctcctaaga
     8521 tcattaacgg catactcacc ataaatgagt cccgaatcgc aactaaatgc cgttgtgcac
     8581 caatttttga tgctacaggt ataaaatgtt tctccataaa tgcaacaaat ttatccataa
     8641 atcttccctc ttttctttac tgtagattac gcgttcaatc gacgatacag atcaatgatc
     8701 tcttttgcta aatctgtaaa agctatagat gtcatcagat gatcttgact gtggaccgtc
     8761 aaaagcgtga cttcaacgtg atttccttgt gcttcttgtg tcagcatctc tgtttgagag
     8821 tgatgtgctt ggactaaaga ttcctctgca tctttgattt tttcatctgc taaagtgaaa
     8881 tcaccttttt tcgcagcagc aatcgcttcc attgcatcgc tttttgcatt tccgccaaac
     8941 atgatcagac ccatgatggc ttctagattt ttttgatctt ccatttattg tatcctccga
     9001 taatttttat tatttgttta ttagtttttc tgcttggtcg agtacttttt caccattcat
     9061 catgccatag tcagccatat tgattacatc taaaggaatg cctttaggtg ctagcttttc
     9121 agcaaattga ttttgcataa agcgtacttg cggtcctaaa agtaaaacgt ctacattttt
     9181 agattctagt tgattatccg catctgaagc agatacagca aagatgtctg cttccattcc
     9241 ttttgcttct gctgcctttt gcatttttgt gactaggaga cttgtactca ttccagctga
     9301 acatacaagc atgattgttt tcttttccat tttaaacacg ttcctttcta tacttacact
     9361 tgtatataag caagtagcgt gccaacttta aaagcgcttt aaaaagttga tatcatcaca
     9421 tttaaggtct ataccaagtt gcatacacac taaaaaagaa agcgcacact aaacacacaa
     9481 attaattttt attacctttt ttcttttaac gtgcacaaaa aaagttgtga cacaaaactt
     9541 gtcacaactt ttttcaaata aacggtattc aaaagcttgc atctgcggta ataagtccaa
     9601 ccaagttaaa aacataagat gttgttttta ctttgctatt cttattactt gatgcaggac
     9661 agctttttca caacctctca ataaacggtg ttcaatcagc tgaccctggg tattagctca
     9721 aaacgctgga ttcacaacct cttttatttc aaataaccag taaatacaat gaacatctac
     9781 aaaaaacttc tatttattta tagatttcgt caggcaaatc caattctctc ttatcaatca
     9841 aagtctgata ccacgaagca gattttttca atgttctgct tcgttcatcg ttcaattcga
     9901 ttcgaactaa tccatatcga ttcttaaatg cattcattgg tgacacacag tcagtgaatg
     9961 cccataacat atagccttcg caatttgcgc cttcttctgt tgctttgatc aagtaatgca
    10021 aatgctgtcc gataaattcg atacggtaat catcttgcac gattcctttc tcatccatat
    10081 actgttcttc atattctctt cccataccgt tttctgttat cagccaaggg atattatgat
    10141 aattttcttt tagatacatg gctatatcat acatgatttt cggatagatt tcccatcccc
    10201 tggaattatt cattttcttc ccaggtaaat caaagacttc ataatatcgt tcaggatgga
    10261 aaggtgtctc ttcattccat tgataacgag gggcgcgaac gcgtttagga taatattgat
    10321 tgattccaag gaaatctact gtatgatgct gaatcgtttc taaatcctct gttgcaggat
    10381 cgaaataaat cgattgtttt tgacacaact ccaggaactc ttctgtgtag tatcctttta
    10441 ccatcggatc aaaaaatact cgattataaa acaaatcata catataagca gctttttggt
    10501 cttctggaga attggatcga gcatagacca tttcaggatt taaaatacag ccaatccttc
    10561 caccataatt tccgctgcgg taaatctcta ctgctttagc cgttgctaaa actttatgat
    10621 aattccacat catccacttt tttgtgtttt gttcatgcgg ccagcgaaca gcatccaaat
    10681 agcaacgcgt ttggggaacg attggttcgt tgaatgtaaa ccagtttttt accctatcac
    10741 cgtaacgatc aaaggcaatt tgagcatact tagcataaaa atcaactact tttcttgaag
    10801 accagccatc atatttttcc aaaagaaaag ctgggacttc ataatgttcc aaacaaatca
    10861 tcggttctac gtctgcttcg atcaaagagt cgatcatttc acttatatgc tgagcatatt
    10921 cttcatctac tatacagttc tcataatcag taaaaaaacg agcccaattg atagaagtcc
    10981 gataatgtgt cagatggatg gatttcatca gatccacatc ctgctggtat tgttccataa
    11041 aattagttgc tacggctggg ccatacccat tatgccaaac aaaatgttca tttttatacc
    11101 agctatctat aaacgaatcc tgtccttctt tttttccttt ccaaccttcc gtttgccaag
    11161 cagaagatgc agcaccaaga ataaaatttt ttggaatagt aattttcatg atttcctcct
    11221 gttttttaga tcccatcatt ttccacacga tttgctactt ttacaaatgg cagatagatc
    11281 aaaatagaaa caataataca aacaagttgc gtaataactg caccgatatt tcctgctgtt
    11341 gccagataag cattgacgat cggcggcatt ggccaaggga ccattacaac tgctttcgcc
    11401 gcaaatccta aagcggttgc aaaataacca atcgttccag tgataagagg cgttaagata
    11461 aacggaatag ctaaaattgg attcagcatg atcggcatac caaaaataac tggttcattg
    11521 atgttgaaga tcccaggagc aagggataac ttggtgattt gtttcatatc ttcccgtttt
    11581 ccaactaaca atactgcaat cagtaaaccg atcgtgactc cagacccgcc catactcata
    11641 tacatatccc aaaatggcat agtaataatg ttcggtactt cttttccagc ttcaaaagct
    11701 tccgtattta cagcaattgc cgctaaaagc aacggttccc gtatcggctt caccatttga
    11761 tttccatgga taccaatcac ccaaaagact tgcgcaataa acatcaatag caaaataccc
    11821 ggtaaccctt gtacgacacc ttctaatggg cgttgtacga cattgtaaat gatatcataa
    11881 aggtacattc ctgtaagttc tttgatgata aatcccgcag ccgcgatacc cgatacagtc
    11941 agaatcgtcg ggaataaagc agaaaaacta gcagaaacat tcgttggcac ggtatcaggc
    12001 attctcagct gtagtttctt ctgtttcctc aaccagcaat aaagttcaat agaaaggatg
    12061 gagaccaaca ttcccaaaaa taaccctttc gtatctgtat attgtttagc caatacgttt
    12121 tctactataa cagtctgatt ttcattcact aattcataaa cagttggatt aacagataaa
    12181 taagacatca ctgcgatgat accagggaat acaccatcca ctttattcag cttggctaat
    12241 tcgatcccta tcagaaaaac agcatagatg gtcaagaaac ttagtgtgac atagctgatg
    12301 gcagatgcga taggctttaa ttcttctaaa aaacgcaaga aacttatctg tgctaagcca
    12361 ttttccgagt caaagaccat tgctgaaaac aatgtgccaa aagcacccgt aataattacc
    12421 ggaatcaatg cagcaaatgc gttttttatc accatgatat accttaaatt attgattctc
    12481 gttgccacgt tccctaaaac attggctaat ttatccataa aactcattcc agatcccctc
    12541 attcctgctt cttctccttg acgtcatctc tttttgtcta ttagtcagcg cttacatttc
    12601 gatgaacaga aactatttac tcctcttttt taccaaatat aaaacaaaaa ttcatctgat
    12661 gtatttatca taaaatacat cagatgaact gtcaattagt ttctttttct cctattattt
    12721 tctgtaattc tctccgattt attgccatat ggacagtcat tgcatcaaac gcagcaagtg
    12781 cattccgcct ttcgatggcc tgaacgatct ctgtatgaat atcgatcgtc tcgattttca
    12841 gatgctggat atcataattt ttattaaaca aagcaattga ttcattaatg attgggataa
    12901 tatgcagcaa ggctacgttt ccgctagctt ctgcaatcat cgtatgaaaa tcgatgtcta
    12961 attgaacatg ttcttgtgtg ccttctcgaa aagaagtttc aatcttttgc gttaattttt
    13021 tcatttggac gatttgattc ttcgttgcat attccgctgc caaacctgcc atcttaggct
    13081 ccaataaata tctcagttca aataagtctc gtatcatccg atcctgatct tcaatcaaag
    13141 aaaaaccgag tgggtcattg gcaatccctc gcttatcact gacaaatgtc cccgctcctt
    13201 gccgaacttc aaggatattc ctggaagcta gagcccgcac agcttctcgc accgtgcttc
    13261 gccccacctc taaataatcg gacaattcat attcattcgg cagtttatct cctacttgca
    13321 actgcatttc ctttaaatat ctaaggatct tgtcagtagt tacttctact agggttttat
    13381 tttcttccaa accacatacc tccttttcat ctgatgtatt ttttcgtaat tattataaca
    13441 cgtttgaatc atctgatgat tttttgtata caaaattgat taatccgttc atagacatac
    13501 aaacgaagaa gatatttacc ataaaaaaga ctaggataaa
//
LOCUS       DAYYGO010000075        13455 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000075 DAYYGO010000000
VERSION     DAYYGO010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.077"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            114..761
                     /locus_tag="ACHA5T_001907"
     CDS             114..761
                     /locus_tag="ACHA5T_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFM8887472.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    890..1106
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1154..3799
                     /locus_tag="ACHA5T_001908"
     CDS             1154..3799
                     /locus_tag="ACHA5T_001908"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFM8887473.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4109..5422
                     /locus_tag="ACHA5T_001909"
     CDS             4109..5422
                     /locus_tag="ACHA5T_001909"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFM8887474.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5412..6065
                     /locus_tag="ACHA5T_001910"
     CDS             5412..6065
                     /locus_tag="ACHA5T_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8887475.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6120..6812
                     /gene="radC"
                     /locus_tag="ACHA5T_001911"
     CDS             6120..6812
                     /gene="radC"
                     /locus_tag="ACHA5T_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFM8887476.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6962..7162
                     /locus_tag="ACHA5T_001912"
     CDS             6962..7162
                     /locus_tag="ACHA5T_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8887477.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(7416..9095)
                     /locus_tag="ACHA5T_001913"
     CDS             complement(7416..9095)
                     /locus_tag="ACHA5T_001913"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFM8887478.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(9097..9309)
                     /locus_tag="ACHA5T_001914"
     CDS             complement(9097..9309)
                     /locus_tag="ACHA5T_001914"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFM8887479.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9702..11432
                     /gene="efrA"
                     /locus_tag="ACHA5T_001915"
     CDS             9702..11432
                     /gene="efrA"
                     /locus_tag="ACHA5T_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFM8887480.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            11429..13189
                     /gene="efrB"
                     /locus_tag="ACHA5T_001916"
     CDS             11429..13189
                     /gene="efrB"
                     /locus_tag="ACHA5T_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFM8887481.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
BASE COUNT         4259 a         2339 c         2895 g         3962 t
ORIGIN      
        1 tttgaacacc gttttatttg tctcacaccc ttttccttcc tacttgtaac cttgctcccg
       61 tgttagaaac atgttacact aaatatgtga aattattaac aaggagcgtc gttatgcgag
      121 aaaaaacaga tacactgcca aaagcaactg cgaaaagatt acctttatat ttacgctact
      181 tgaagatgct ggatgactca ggtataagta gaatcaaatc aaatgagttc agcgaaatca
      241 cccaaattcc ttctgcaacg atccgcagag acttctcgca attaggcgag ctaggcagaa
      301 gcggatatgg ctatgatgtc ccttttctta tcgatgtatt caataatatc cttaatacta
      361 aagaagaaaa acgaatcgca ttagtaggat atggaaatct tggcaaagca ttgaaacaca
      421 acaattttag aagaaatgaa aatctaaata tcgtgtgcgt ctttgataat gatccagcac
      481 tgatcaatcg agtaattgat ggagaaatga tctatccgat tgatcgtttc gcggagatag
      541 ccaaagcaaa aaatgtgaca gtagcaatct ctacggtacc aagtaaatat tcacaatctg
      601 ctattgatga aattgtcaaa ggaaatgtaa ccgctatact taattttgcg cctgacagag
      661 taactgtccc agcttatgtc aatgttcaat acattgattt gacaaccgag ttgcagacgc
      721 ttatttactt tgatgagaat tactcagaag ttttttctta ggaaaatttg agtaggctta
      781 ttgatcttgc gagaactcgt cgtaattctt tcaaaaattt tttagtacta aatgaagtgg
      841 ttttttcacg accacttgtt gactaaaaaa aaaaaccatt gtagaatgaa gcataacgct
      901 gaacaagagg agtaactggc acattcattc ttagagagag aactatctgc tgaaaggttc
      961 tcaatgacaa ccagtgaagg tagcttggga gtttctgtct tgaatggagt agagatagac
     1021 gagtcgtgat cgttaactca cttgagaggc aataatgatt gattattgca atttaggtgg
     1081 taccgcgtaa atcatacgtc ctagaatacg aaagtatttt aggacgtttt ttagtttaaa
     1141 ggaggaaaac agcatgacgg aagaaaataa cctatcaaca aaatataatc cgcaagaagt
     1201 tgaagctgga cgttatcagc attggttaga acaagatttg tttaaaccga gcggagataa
     1261 aaaagcaaaa ccttattcaa tcgtaatccc gcccccaaat gttactggaa aacttcattt
     1321 agggcatgct tgggatacga ccttgcaaga tatgctgatt cgtcaaaaga gaatgcaagg
     1381 atatgatact ttatggctgc ctggtatgga ccatgcagga atcgcgacac aagcaaaagt
     1441 agaagcgaag cttagagagc aagggatttc tcgttatgat ctaggtcgtg aaaagttcat
     1501 cgatcaagtg tgggaatgga aagaagagta tgcgagccac atccgcgaac agtgggctaa
     1561 aatgggctta tctttagatt acagtaggga acgttttaca ttagatgatg gattatctga
     1621 agcagtacgt aaagtttttg ttaccttata cgaaaaaggt ttgatttatc gtggtgaata
     1681 tatcatcaac tgggatcctc aagcacgaac agctctatct gatatcgaag ttattcataa
     1741 agatatcgaa ggcgcctttt atcatatgag ctatgaacta gcagatggat caggcgtagt
     1801 agaaattgcg acaacccgtc cagaaacgat gttgggagat acggcgattg ctgttcatcc
     1861 agaagacgag cgttatcaag cgctcatcgg caaaaaagtg attctacctt tagtcaataa
     1921 acaaatccca atcattgcag atgaatatgt agatatggaa ttcgggacag gtgtagtaaa
     1981 aatcactcca gcacatgatc caaacgactt tgaggtaggt aatcgtcatg atcttccacg
     2041 cgtaaatgtt atggatgatg atggtacgat gaatgaacta gcaggaaaat atgctggaat
     2101 ggaacgtttt gctgctcgta aagcaatcgt gcaagatctg aaagaaatcg gtcgattgat
     2161 taaaatcgat acgatgatcc acagtgtggg tcattccgag cggacaggtg tcgttgttga
     2221 acctcggctg tctactcaat ggtttgtaaa aatggcgccg ttagcgaaaa aagccattga
     2281 taaccaagaa acagatcaag cagtggaatt cttccctcca agattcaatc aaacgttttt
     2341 acgttggatg gaaaatgtcc atgattgggt aatctcacga caattatggt ggggacatca
     2401 aattcctgca tggtatcaca aagaaacagg tgaaatgtat gtagggatgg aagcaccagc
     2461 ggatagtgaa aactgggtac aagatcctga tgttttagac acttggttta gttcagcttt
     2521 gtggcctttc tcaacaatgg gctggccaga tacggaaagc gaagattata agcgatactt
     2581 ccctactagc actttagtaa ccggttatga catcattttc ttctgggtaa gcagaatgat
     2641 tttccaaagc ttggaattta ctggcgaacg tccatttgag aatgtcttga ttcatggatt
     2701 gattcgtgac gaacaagggc ggaagatgag taaatcatta ggtaatggaa tcgatccgat
     2761 ggaagtaatc gaaaagtatg gagcagatgc tctccgctgg ttcttatcga atggttctgc
     2821 accaggacaa gatgtccgct tcagctatga aaaaatggat gcttcttgga actttatcaa
     2881 caaaatctgg aatgcttcac gtttcgttat catgaatatc gaaggaatga ccgtagaaga
     2941 gatcgattta tctggagaaa aatcagtggc tgaccggtgg atccttactc gtttgaatga
     3001 aacgatcgaa cgagtaactg agctatttga tcgttttgaa tttggtgaag caggtcgaca
     3061 actgtataac tttatctggg atgatttttg tgactggtac atcgaaatga gtaaagaagt
     3121 actttatgga gatgatgctg tttcaaaaca aaccacccgt agcatcttag tccatacact
     3181 tgatcagatc ctacgtttat tgcatccaat catgccattc gttacagaag aaatatggga
     3241 acatattcct catcagggaa attctctagt agtggcagaa tatcctgttg tacgtccaga
     3301 gtttgatgat gaaacagctt ctaaagggat ggaagtattg aaagaattga ttcgagccgt
     3361 tcgaaatatt cgttcagaag tgaatacacc attgtctaaa ccaattacgt tgctaattaa
     3421 aacaaacgat gctaaaatcg atcaattttt agttgaaaat acaagctata tcgaacgctt
     3481 ctgtaatccc gaagagttga cgatatcaag tgagatcact gcgccagatc tagcgatgtc
     3541 agctgttctg acaggtgcag aaatcttctt gccgttagca ggattgatca atattgaaga
     3601 agaaatcaaa cgattagaaa aagaactgga aaagtggaca agcgaagtaa aacgtgtcca
     3661 aggaaaactt tctaatgaac gttttgtttc taacgcgccg gaagaagtag tagaggcaga
     3721 gcgtgcaaaa gaaaaagatt atttagagaa gcaaacggct gtcaaagaac gaatcgaaag
     3781 tttgcgtact attcaataag aatagaaaaa gagagtgtga aatcagctag ctattactaa
     3841 aagctagctg attgaacatc gttacaggaa agagtttgag cctcactctt agcttcgatt
     3901 cataaggaga tgtgacataa attttgtcgc atctcttttt tagtctactc ttattatgta
     3961 ggagaaggag ggaggtggat catgtatcaa gagtcaataa agtagttgat aaaatcgttc
     4021 ctctctctgt ttcttttctc tttgtcatac ttttattttt cttatgtgtt ctatttgtta
     4081 taataaaaga gaaatggggg aattgttgat gacgattgaa gaagcaatag aatggatcca
     4141 tagtcgatta ccatttggtt cacgtcctgg ccttgaccga gtcgaagctt tattagaagt
     4201 ggtagggcat ccagagagaa aagtaaaaac gatccatatc gctggaacaa acggaaaagg
     4261 ttctactgta gcttatttga gaagcctatt agaagaggct gggctgacag taggtacatt
     4321 tacttcacct tatatcgaaa cgttcaatga acggatcgca atcaatggca aaagcattcc
     4381 agacaatgat ttgatccagc ttgtaaaaaa atatcagcca ctgatcaaac aacttgatca
     4441 atttgaagca gtagctggta ttactgaatt cgagacatta acagcaatgg catttgatta
     4501 ttttttgatg aagaaagtgg acgtagcgat tatcgaagtt ggcttaggcg gcttattgga
     4561 ttctaccaat gtagctgtac caatgctgac gggaataaca acgattggac ttgatcatac
     4621 cgaaatactt ggagatacaa ttgaagaaat cgctgcacaa aaagctggaa tcatcaaaca
     4681 aggcgttcca gtagtaacag gaaatattgc cccagaagct tttactgtca tccaacaaac
     4741 agctaaagca aaaaatgcac cgctttatgt atacggggaa aattataaaa taaactatct
     4801 gcatcctgat gagatgtggg gagaagtatt tgaatttttt aatgaaatag gaaagatccc
     4861 tcaattaacc actccgcttt tagggcaaca tcaagctgaa aatgcaggag tagccatcga
     4921 attgtatttt gtttactgtc aattggaaaa gctgcctttc cgagaaaaag atgttttgaa
     4981 gggattgaag aaatctcgat ggccaggaag aatggagcgt ttgagtaacg agccattagt
     5041 tgttttagat ggtgcccata atacgcatgc aatgaaccgg cttgttgaga atttgaaaaa
     5101 agaattcaaa ggatatcaaa tcaacatttt gttttccgcc ttacgaacta aaaatgttag
     5161 agaaatgcta gaacgattgc agcaagtacc aaatgtccat atttatttga cgacctttga
     5221 acatccaaaa gccatcgaat taagtgaagt atcagattta gcaagtgaga gagtatcaat
     5281 cgtttcattg tggcaattcg gattagcaga tatattggaa aagatgacaa atgaagacgt
     5341 attagttgtg accggttcac tttatttcat ttcagaagtt cgccatttat taatacaatt
     5401 aggaggagat catgaagtat aatgcagtga tttttgacat ggatggtttg ctgttcgaca
     5461 cagagttagt gtattacgaa gccacacaag tagtagcaga caagatgggt tttccttatg
     5521 ataaagacct gtatttaaaa ttcttaggtg tttcagatga agaagtatgg gcgaattatc
     5581 atatgatttt ctcggaattt ggcaaaacaa gtgtacaaag attcatagat gattcatacc
     5641 aggaaacgct tgaacgtttt tcgcaaggag cggtacaaat gaagcccggt gtgttggaac
     5701 tcctctcttt tttagaagaa catcagattc ccaaagttgt cgcttccagt aaccaacgag
     5761 cagtcattga acttttattg aagaagaaca atctgtttga acgatttgag acgattgttt
     5821 ctgcagaaaa tgttcaacga gcaaaaccag acccagaaat tttcttgtta gctaatcaga
     5881 agctaggtac agcgaagaaa gaaacattga ttttagaaga ttcacaaaat ggtattttag
     5941 cagcttctgc ggctgagatc ccggtagtca tggtaccaga tctgcttcca ccatcccaag
     6001 aattgatcaa aaaaacagct ggggttgttt cttctttaca tgaaatccct gcattgttaa
     6061 aataaaaatc catctctttt cgtatcttat tttagataag atagaaagga gatgtttttt
     6121 tgggaaaacg attgatcaaa gaagtaccta ctagctcttt accaagggaa cgtatggaaa
     6181 tttatggcgc ggaagcttta tcggatcagg agcttttggc aatcttactg cgtaccggtc
     6241 aacaccctta tagtgtgatg tcgattgctg gaaatctgtt aaaaacgttt ggaggactgg
     6301 cctctttacg acaggctact ttgcatgagt tagaagagat tcgagggata ggacgcgtga
     6361 aagcaatcga aatcaaagcc ttgatcgaat taggtcgacg aattcagacg gatcatgaaa
     6421 gagcggctcc accggttcgt accagctacg aattggccca gcaattgatt ctggaaatga
     6481 aagatcacaa acaagaacat ttggtttgta tttatttgga cacgaagaat caagtgatct
     6541 taaaaaaaac agtgttcatc ggttcgctga atcagtcagt cgctcatcct agagaaattt
     6601 tccattatgc tgtacgttat tgtgcagcaa ggatcgtttt agcgcataat catccttctg
     6661 gtaatgttat tccatcgcag caggatatga attttaccaa aaggatacaa aagtgtggag
     6721 aaatgatggg gatcactgta ctggaccact tgatcatcgg acgcaaacga tattttagtt
     6781 tgagagaaga aggaatgatg gaagagaagt aagttgcttt cttgcaaaaa aaatgaagca
     6841 agtgtacaat tatattgtaa ttttgtttgg gaatagaagt tgaaacatct gttgcacctc
     6901 tatcgtacac gagcaatgtt acaatttaaa ttagtgcttt tggcacgagg aggatattat
     6961 tatggaaaac ggaacagtaa aatggttcaa cgctgaaaaa ggatttggat ttatctcacg
     7021 tgaagatgga agcgacgtat tcgttcactt ctcagctatc caaggtgacg gattcaaaac
     7081 attagaagaa ggtcaagcag tgactttcga cgttgaagaa tcagaccgcg gcccacaagc
     7141 tgcaaacgtt gtaaaagctt aattttatct gtaaatcaaa ttgatggtag acatcaattt
     7201 ttacgagaga acaaagcgag taacaaaaat acctgccaca agagacaatt tctcttgtgg
     7261 caggtatttt tttgtatgaa aaagtaaata aatttaagag gctatgaaag agccaccttg
     7321 tatcaaggaa taagaacaaa caaaaatggc ttctcatgtt tttcatttgt tttctcttat
     7381 attcaggaca aggtgctgat ttcataacct cttatttatt cttcatctgg tgtcattacc
     7441 attggcaaaa tcattggatg tctttctgta cgttcgaata agaaaggctg aagcgcgcca
     7501 gtcatcgctt ctttcaattt atattctgaa cagtcaggtc cattcaaaca ttctcttaaa
     7561 gcgtggaata agattcgctg agcttcattg atcatttctc ctgattctcg catgtagaca
     7621 aatccacgag agagaatatc tggaccagcc aagatttctt ttttcttgat atccactgtc
     7681 gcgacagcta atacaagacc ttcttctgaa agcaggtgac ggtcacgtaa aacgacatta
     7741 ccaatatcac cgatcccgct accatcgata tacacatctc cagccacaaa atgcccagca
     7801 ggacgcgcag aatcgctagt taacgctaaa acatcgccat tttccatgat gaagcagttt
     7861 tctgcaggaa cacctacatc ttgtgctaga gaagcatgta ctttcaacat gcggaattct
     7921 ccgtggacag gaataaaata ttttggcttc atcaaacgaa gcattaattt ttgttcttct
     7981 tgtccaccgt gaccggaagt atggatgtta ttgatttttc cgtgaatcac ttctgcacca
     8041 gcttcagaca gcaaattgat caaatgattg acacttgttg tattaccagg aataggtgaa
     8101 cttgaaaaga tcactgtatc gtctggctgg atttggattt gacggtgagt accattcgcg
     8161 atgcggctca acgcggccat cgcttctcct tgagaacctg tacataggat catcgtttca
     8221 ttggcaggca agcgattgat ttcgtgtgca tctacaaatg tgccgtctgg aactttgata
     8281 tatcctaaac gttgaccgtt gacgatggca ttttccatgc ttcgtccgaa aacagcgatt
     8341 ttacgaccag ttgccacagc ggcatctgct gcttgctgca aacggaagat gttagaagca
     8401 aagctggcaa aaatgatacg tccttcgatt tttgaaaaga tcttcaaaat agaatgccca
     8461 atcgtttttt ctgatttagt aaatgttgga atctcagcat ttgtactatc agataaaagg
     8521 cacaagactc cttcttcccc gatacgagcc attttatgca agtttgctgg ttcgccaaca
     8581 ggagtgaaat cgaatttgaa gtctccagtc gctacgatat ttccagaagg tgttttcacc
     8641 acgatcccta atgcatctgg gatactatgg gtcgttctga aaaagctgac agaagtcttt
     8701 ctaaaacgga tgatcgtatc ttcattgatc tcatggagct ctgcatctcg aagcaagcca
     8761 tgctcatcta atttgtttgt gatcaacgct aatgcgagag gaccagcata gataggaata
     8821 tttgcttgtt tcagcaagta gggtattccg ccgatgtggt cttcgtgccc gtgagtaatc
     8881 accaaggctt ttactttgtg taagttttgt atgatgtagc tgtagtcggg aatgacatag
     8941 tcaattccaa gtaaatcgtc ttccgggaat ttgatcccgg catcaatcaa aatgatttca
     9001 tcttggaatt gtaccccata cgtatttttc ccgatttcac ctaatccacc aatagcaaag
     9061 acgcctgtct cgttattttt gatggaaagt ttcatattaa aactccacta atgagaaatc
     9121 agcattttct ttttcgtact ctagatgact tccttctaga agttgaacga attcaatatt
     9181 gtatggggta ttttcttcca cacgttggcg agcgtctaca tcgctttttg cctcaatgta
     9241 aagagatttt gtttcttccc gtttagggtt gcgtactttt gtttcttgat aataaacttt
     9301 atagatcatt gttttgtctc ctttatctgc gtaaagcagt gatttagcta gttgattttt
     9361 ttagacaaca caaaacgagg ggacaagcca cctggttttt catatgcttt gactagaata
     9421 ctggattcga gtcaaagaga caagcctcgt ttttgcttat ctgttcggat attttttcaa
     9481 ccgtaccttc tgtataaaaa atatacaaca attaattgtt attctagcat aaaacagcaa
     9541 ataagtatag aaatcaacta gattgaaaga aaaaatcaat gtttatcatc ccgctataat
     9601 cataatgact tttttatgag agaagtgttc tattaatagg ctcgttgtgc tatcattttt
     9661 agtaatgaaa atgtaacaaa agattaaaag gagatgagaa agtgaaacta atgtggcatt
     9721 ataccatgcg ttataagaaa ttcttgtttt tcaatttcat atgcgtattt ggttttattt
     9781 tgattgaatt aggacttcct acgattttag cacgtatgat cgatgtagga atccgcaatg
     9841 atgattttga ttatgtgaag caacaaggga tatttatgat cgttattact gtaatcggta
     9901 ttattttgaa tatcttgtta ggttattttg gttcgaggat tacgactaat atcgtagcag
     9961 atattcgaga tgatttgttc gaaaagatcc aaagttattc gcatcaggaa tacgaaaatc
    10021 taggcgtatc ttcattgatc actcgtacaa caaacgatgc ttatcagatc atgttgttcc
    10081 tgcaaaatat tttacgtatc ggttttatgg caccaatgat gttcattgtc agtttgtaca
    10141 tggtcatgcg tacaagtgtg acactcagtt tgtatgtagt aggtgcatta cctttactat
    10201 tattggcagt cgtagcgatt gctaaggtgt ctgaaccgtt atctaaaaag caacaaaaaa
    10261 acttagataa aatcaacagt atcttgcggg aaaatctttc aggattgcgt gtgattcgtg
    10321 cattcgtcaa tgaaaagttt gaagaaaaac gttttgctgt agtcaatgag aattatgctt
    10381 ctagttcaaa aagcttattc cgtttgatgg ctgttgcaca accgggtttt tatttcttat
    10441 ttaatatcgt aatggtattg atcatctgga gcggggcagt gcaaatcgat gcaggcgagc
    10501 ttcgtgtagg agatttgatt gcgtttatcg agtatatatt ccatgccttg ttctcattca
    10561 tgttatttgc tagtgtgttc atgatgtatc caagagcagc tgtttcagct ggacgtatcc
    10621 aagaagccct agatgccaaa ccttcgattg tagaagatcc aaatggtata tctgatacaa
    10681 aaactaaagg gtatatcgaa tttcaaaatg taacatttgc ttatccaggc cattcgcaag
    10741 agccagtgat acgaaatgtc agttttacag cctctccagg agagaccgtt gcttttattg
    10801 gaagtaccgg tagcggaaaa tctacgttga tccaattgat ccctcgcttt tatgatgtga
    10861 gtgaaggaaa gatcttgatc gatggagtag acgtgagaga ttatcggcta agcaagttgc
    10921 gagataagat tgggtttatt ccgcaaaaag cgctgctatt tactggaacg attgcagata
    10981 atttgcgata cgggaaagaa gatgctacgc aagaagaaat ggaacgtgca gctgagattg
    11041 ctcaagcttc agaattcatt tctcgaaaac cagatggatt taatgaacat ttatcagaag
    11101 gtggttccaa tttttcaggt ggacaaaaac aacgattggc aatagccaga gccatcatac
    11161 gccgtccgga aatctatatt tttgatgaca gcttttctgc attagattat caaacggatg
    11221 cgaagttacg tgctcgtctg aaaaaagaaa cgacagaatc tactgtattg atcgtagcac
    11281 aacgtgtggg aacgatcatg catgcagata agatcatcgt attaaatgaa ggagaagtcg
    11341 tcggtatggg gacgcatcgg gagttgttgg aaaaatgccc gatttattat gatattgccg
    11401 cttctcaatt atcaaaggag gaattagcat gaaaaaaacc ttttcttcct taaaacgttt
    11461 agctcgatat atcagaccgt atcgtgagac ttttctttta gtgatcctat ttacagttct
    11521 gacagtggct ttcaacgtgg ctttgccata tgttacagga cttcctacta cagaaatcag
    11581 ccgtaatatt gccaatggcg aatcaatcaa tttttcttat atattcaaat gtctcatttg
    11641 gatcacagtt gttggaatag ggtattgtat ttcgcaatta ctttctggtg ttttgatgac
    11701 aaatgtcgtt caaagtgcca tgcatgattt gagaagagac atagaagaaa aaatcaatcg
    11761 tcttccggtt tcatatttcg ataaaaacca gcaaggaaat attttgtctc gtgtgacgaa
    11821 tgatgtagat gcagtcagcg gtgctttaca acaagctttt attggtatcg taaatgcgat
    11881 attgggaatt acgttggctg cagcaatgat gttttatatc cagccgatga tggcgctgat
    11941 ctctatgatc atgattccaa catcgatctg gatctcaaaa cgagtgatca atgcttcgca
    12001 gaaatatttt caaagcatgc agaattctct tggtgagcta aacggctacg tccaagaaaa
    12061 tatgacaggt tttagtgtat tgaaagtata tggacgcgaa aaagaaacat tggaaggatt
    12121 caaaaaagtt aatcacagct tgaaatactt tgggtttcgt gcagcattca tttctggctt
    12181 gatgatgcct ctagttcaat taactgccta tgctacttat attggtatgg ctgttctagg
    12241 aagcttttat gttattactg gggcgatcgt tgtcggtcaa ctacaagcat tcatccaata
    12301 tatttggcaa atcagtcagc caatggggaa cgtgacacaa ttgtcttcag ttttgcaaag
    12361 tgcatcagcg gcaacgaaac gtgtattcga gattttagat gagccagaag aaaaagaaaa
    12421 caaagtagat gcttcactgc cagaaacgat tttaggagat gtgacattcg atcatgtcga
    12481 ttttgcatat gatccgaaga aaccattaat ccaagatttg aattttgaag tgaaagctgg
    12541 tcaaaccgta gctgtggtcg gtccgaccgg tgccggcaag acgacattga tcaatctatt
    12601 gatgcgtttt tatgacgtag acaaaggagc aataaggatt gatggaatcg acaccaaatc
    12661 aatgagtcgc agtgatgtgc gttcgctatt tggtatggtc ttgcaagatg catggcttta
    12721 tcaaggaacg attgcagata atatccgctt cggaaagctt gatgcgacag attatgaagt
    12781 agttgatgct gcgaaaacag caaacgtaga tcattttatc cgaacgatgc cagatggata
    12841 tgatatggcc atcaattctg agggagacaa tgtgtcttta ggacaaaaac agttgctgac
    12901 gattgcccga gcagtggtct ccgatcctaa aatcttgatt ttagatgaag caacaagttc
    12961 agtagacacc cgtttagaag cgttgattca aaaagcaatg gatcgcgtga tggagggacg
    13021 gacaagtttc gtcatcgctc accgtttatc gacaatccgt gatgcagatc tgattctagt
    13081 gatggatcaa ggtcagatca ttgaaaaagg aacgcatgag agtttattgg cacaaggtgg
    13141 attttatgaa aaactgtata acagccaatt tgctgaggaa gcagaataag tcatataaga
    13201 ataaaacagt ttaaaactta ttttttgttc tctattcttg ataatggtaa agtaaagtta
    13261 tcaagaaaag tgaggacacg gagatggaac agtttttaaa tgaaaaaatt actgaagcca
    13321 aaagagaagt tgatcgttta acgcacgtct gtcagacgaa gccttttgat tcagctgatt
    13381 tttttgaaaa tgagtttcaa atcgaacgct tggcagcaca aatagaagtg tacgaattaa
    13441 ttttaaaaaa agtaa
//
LOCUS       DAYYGO010000076        13233 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000076 DAYYGO010000000
VERSION     DAYYGO010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.078"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..304
                     /locus_tag="ACHA5T_001917"
     CDS             5..304
                     /locus_tag="ACHA5T_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887482.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(401..835)
                     /locus_tag="ACHA5T_001918"
     CDS             complement(401..835)
                     /locus_tag="ACHA5T_001918"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8887483.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1067..1777)
                     /locus_tag="ACHA5T_001919"
     CDS             complement(1067..1777)
                     /locus_tag="ACHA5T_001919"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFM8887484.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(1767..2627)
                     /locus_tag="ACHA5T_001920"
     CDS             complement(1767..2627)
                     /locus_tag="ACHA5T_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFM8887485.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(2632..3270)
                     /locus_tag="ACHA5T_001921"
     CDS             complement(2632..3270)
                     /locus_tag="ACHA5T_001921"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFM8887486.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3285..3584)
                     /locus_tag="ACHA5T_001922"
     CDS             complement(3285..3584)
                     /locus_tag="ACHA5T_001922"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8887487.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(3627..5111)
                     /locus_tag="ACHA5T_001923"
     CDS             complement(3627..5111)
                     /locus_tag="ACHA5T_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFM8887488.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5131..5592)
                     /locus_tag="ACHA5T_001924"
     CDS             complement(5131..5592)
                     /locus_tag="ACHA5T_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8887489.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(5610..6677)
                     /gene="ulaG"
                     /locus_tag="ACHA5T_001925"
     CDS             complement(5610..6677)
                     /gene="ulaG"
                     /locus_tag="ACHA5T_001925"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFM8887490.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            6895..7665
                     /locus_tag="ACHA5T_001926"
     CDS             6895..7665
                     /locus_tag="ACHA5T_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8887491.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(7938..8765)
                     /locus_tag="ACHA5T_001927"
     CDS             complement(7938..8765)
                     /locus_tag="ACHA5T_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8887492.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9333..10568
                     /locus_tag="ACHA5T_001928"
     CDS             9333..10568
                     /locus_tag="ACHA5T_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8887493.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(11567..11827)
                     /locus_tag="ACHA5T_001929"
     CDS             complement(11567..11827)
                     /locus_tag="ACHA5T_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFM8887494.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(11854..13155)
                     /locus_tag="ACHA5T_001930"
     CDS             complement(11854..13155)
                     /locus_tag="ACHA5T_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8887495.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
BASE COUNT         4198 a         2759 c         2081 g         4195 t
ORIGIN      
        1 aaacatggat tatactgttg aatttgacag tgaactactt aagcaatttc cacaagaaga
       61 acatttcatg tataaagaag caatttccca caaagtgaca gagtatttgg agcataattt
      121 ttttcgtata aagccagtgc gtactcgttc ggcaaaagga atatacgaga tgaaagtcaa
      181 aattaataaa aaaaattatc gcatagcatt tgctttaaaa aaaaatcatg tgttcatttt
      241 ttatattagt tcgaatttgc aaaaaattgt atttgataaa gaggtgagta aaaaaataaa
      301 atagctgagc attggaggat agattgagtg atactaagtc tatcctcttg tttttctaaa
      361 gaactaagct agaatttttt cttttgtacg ttattatatg ttatttgtca ttaccaaaaa
      421 gcaccttttt tcttctagta tctatgacat aagaaatacc acccatttcc tttacttgaa
      481 caaatcctac tgattcataa aatttttttg ttttctcagt atcatctgtc aataattcga
      541 tttggactgg tccataacta gtgatcttct ccaaagcttc attcaaaagc actttgccta
      601 ttccttggcg ctgatacctc ggatgaatga ggatatcttg aataaatgcg atcgtcgcaa
      661 aatcagtgat ccaacgaacg attccgataa tcttcccttc cccattacgc tttatcagag
      721 attcagattg ttcaaatgct ctagctaacc ttgcagtgtc ttttgtgtaa ttcgtccaac
      781 caacgctctt atataattca gaaatctcat cgatcataag ggaatcaata ttcatttttt
      841 tcttcctttc ttatacaatt ctccctctaa taatacagtt ctttgactat ttaatctatc
      901 acatataact ctcctgtaaa gattctctcg attccttaaa attcctcttt ccttttagaa
      961 tgcatacttt ttgataaata aaaaggttgt gataaatttt cttcacaacc tctgtttctc
     1021 tctatcaagt gttatctttt aattttctac ccttacccta gcatctctat tgcccgtagt
     1081 atgcatactt tccatgtttg cgaaaataat gtttatctag taaatattgc gggatttcac
     1141 atatattggg gttgatcagc tcgttttctt tggccatcag acaaatttca tctaagatca
     1201 aactgttttc tactgctttc ataggtgaat ccccccatgt gaacggtcca tgtccataga
     1261 caagtactcc agggacttcg tttggatcta attttctttc cttaaacgtc tcgacaatca
     1321 cattccctgt atgaacttcg taagcttctc ttacttcttc tttcgttaat tggcgtgtac
     1381 aaggaacctt accataaaaa gcatcagcat gagtcgtgcc ataagctggc aaatcgcgtc
     1441 ctgcttgcgc ccacatgacg gagtgcgtgg aatgagtatg agtaatagca gttatatctt
     1501 caaaagtttg atacaaaaca acatgtgtgg ggagatctga agatggtttt aagctatctt
     1561 cttctattat atttcccgac aaatctgtta ccaccatttg atctgcttgc atgcattcat
     1621 atctgacacc gcttggctta atgacgatga ctcctagctc acggtccact tcactcacat
     1681 tgccccaagt caatttgact aatccgtatc gcggcaatgc cagatttgct tcaaggacac
     1741 gttgcttcat tgctgcaatt ttttcttcac aagacatcat agccagcctc cttcaattta
     1801 gaacttaagt agtcttttgc ctgctggatt tctcttcgaa agtcttggct tttttcactc
     1861 cacatttcaa tcagaaatgt tccatcataa tctagacgtt tcaaatgtct taataatcca
     1921 agaaaatcaa cacaaccgct accaaaagga acatcacgaa actggcctga atattctttt
     1981 gttacagggt aagtatcttt taagtggata gcagtgatat aatcgatccc tttctcaagt
     2041 tctcgagccg gattattttc cggccatgcg gacaaattgc ctaggtctgg atatacttgt
     2101 agaaaaggtg aatgaatctg ttctttgatt tctaaaaatt ttgaaatgga gttcataaat
     2161 ggatcatcca tgatttccac ggaaagaatg atgccatatt tagaagcttc ctttacgctt
     2221 tcttttattc cttcgataaa gtcttctctg gaattcattg atttctcttc ataataaaca
     2281 tcgtatccag ctatttggat tactttgacg cttagattat gcgccaaatg gattgctttt
     2341 ttcattagtt gtttcgctgc ttttttcttt tctggatcag cagaaccaaa cggaaatcgc
     2401 cgatgcgcac ttaaacaaat cgaatggata cgtacaccgg agctgaacat ctcttctctc
     2461 agctcagcta tcttttcttc agaccaatct aatcgtgcta accgctcatc cgtctcatca
     2521 atcgacatct cgataaaatc gaagtccatt tcttttacaa gggaaaaacg ttctttccat
     2581 gaaatatctt tgggtaacgc tttttcataa atcccaattt gagccatttc cttaccccca
     2641 gatacgaatg atctcatctt tgaagttttg tgcggccttc atcggatcat ttgctgctgt
     2701 aataccacga ccagtgataa aggtatacac atcgatccct tcaaataagt gtaatgtgtg
     2761 tggatttaat ccaccagtta cagaaacatt aaagcctagt tcaatcagtt ttttgatctt
     2821 cgataaatct ttttcacccc aagtttcacc agataacagc gcatcacggc tttgatggta
     2881 gattacctgc cggataccga tgttatgcca atccatggct tgttcaaaag tccaattgcc
     2941 gtagagttct acttgcaatt cacctatttc tttttgtgct gcctccatag ttggaatcgt
     3001 tgcacaacaa atgactgtca tccagtctgc tcctgctttt gcaacattac tcgccactgt
     3061 tcctccagcg tctgcacatt tcgtatcagc gactagtttt ttatctggga acatactgcg
     3121 aatacagcga atcgcttttt gtccttcctg taagcaaaga attgtgccca cttcaataat
     3181 atctactaca tcaccaactt tcataacatc tcctaatgca tgttctaagt cattgtgatc
     3241 taacgcaatt tgcaaattcg gcctgctcat cttagttcct tcctttactt gtttttttgg
     3301 taccactttg tttcagcaaa tttttcactg atttctttga ctgacatcac atttttgata
     3361 ccgataactg taactccttt tttctctgcc tgagtaaaca tattcaaaaa attgactggc
     3421 gtaaaaacta catcgtattg gccagctgaa ctttttccct ctgaaatggc acaatgatgg
     3481 attttggtaa ttggtacgtt tagttctttc attgcttttt ccacacttct catcatcatc
     3541 aagcttgttc ctgatccatt tgcacacgat actaaaattc tcatcttaat ccctccatta
     3601 tgagtgttgt ctaatcagat tttcttttat actctctgct tttctgcata tgtgtcatag
     3661 tcttctacaa tcaaaaaata accatcagga tttttccgat attgtaactg cggaatagcc
     3721 aataaaccaa tgacaactac ggcaattcca gcataaccta ggactttcat gattactgta
     3781 aacaatggcc aagctgttgc ccagtcgaac atcccgatat aaccaccgta tgttgacatc
     3841 ccaatccatg tagcgaataa tgctgaaccg cctacttgta tgattcctga aagaaacggg
     3901 aacaggcaag cggctttgaa tccacctcgg ttattggcat aaactgctat aacggcatta
     3961 tcaaagaaca acggaatgaa accggctatg acgatcgttg gtgatttcaa taaaatcagt
     4021 aaaccaatca tcaaaaactg tcccaaagca ccaaacaaga acccaatcgt taccgcattt
     4081 ggcgaaccaa agccaaaagt agcagctacg tcaattcctg gaacagctcc tggtaaaatc
     4141 gtgtttgaga ttccttggaa agattgagtt agttcatcga cgaatgtccg aacacctaat
     4201 tgcaagatcg ccagatatac cgcaaaattt aaagaagtcg tcataatgta gaataagaaa
     4261 ctctgccctt cttgcatgaa ttgcgcttga atcaaataat ctttgcctag tacaatcaga
     4321 atgatcccaa agaaaaatag catgagtaac gaagtagcaa ccatattctc attgaaaatc
     4381 gacaagaatc ctggtaattt gatatcttca atttttttgt cttgcttgat tgatctattt
     4441 ttcttttcat cccgcttttt catccactca gctaattttg caaatataaa gattccgaac
     4501 atttgctgat gtgccactgc aaatccggca ccttctgtca attcctgcgt gattcctact
     4561 gttaagttag aagctactgt ccaataacaa cctaaaatca gtcccataaa aatcaatgct
     4621 tgtatcctac ctagatcagg aaagcaaaac agtaaaatcc aaaaagcggt agctgcttgt
     4681 tgaacctgaa cgtttccggt agtgaaaacg gaacgcagtt tcgtatattt ctgaaaacgg
     4741 actaataaga tatttaaaac aaatgcaatc agtaataaaa tcatcacatc tccaaaggta
     4801 cggccaaatg tctccattaa tccagcatcc acagcatttt gtccaaaata aggatctgta
     4861 accatggcat ccaaattgaa ccgttccttt agtccaacca agattggccg aaaattattg
     4921 accaaaccac cagagccaac agtcaaaata aagtaaccga ccgttgcctt taagaatcca
     4981 gcgatgcatt catgaagcgg gcgtttcagc aaaatatagc ctaataacac gataaaacca
     5041 atcaggtatg ctggctgcgt caagatattt gttgcaaaat acgtccaaat gcttaacaaa
     5101 aattccccca tcttttttct cctctatcaa ttaattatat tctgccatca caacttggta
     5161 atcatcgatg gagttcgtag agatgagacg ctcaaccaat ccatctgtca ttaataattc
     5221 ggataatttt tggatatttt ctagatgttc ttctggatct tttgctgcga gtgtaaaaaa
     5281 caaactagcc gttttctcct cattctcccc ttcactttca aaacggattg gctgtttcaa
     5341 cttggtaaat gaaatagctg ttccaaaaac accatcactt ttttctgaag aatgcggcat
     5401 tgctacttga ggtgcgatca caatataagg accgtatttt tctacacaat ggatgatctc
     5461 ctccacatac gtttcatcaa taatttcctt atcgatcaat acaagacagc tttcggcgat
     5521 agcttcacgc caatccgtga tttctttatc tgggtaatga atcaattgat ggtcaaaaaa
     5581 gtatttcagc aagaaactcc ccttctttct tataaaaacg acggaaacgg tgtatcattt
     5641 tcaatcgaga agacatcttc aaatcctcga taaaaattaa actcaagatc atctttgttt
     5701 gtcggataga tgaatttccc gcctacttgc cagatataag gtttaaatcc atacttcagg
     5761 cgatcctttt tgaatttcca aatttcagtg atctcttttg gatcagccat aaaattagac
     5821 caaatatcgt agtggacagg aatcactact tttgcattca atgactccgc catgcgcagc
     5881 atatcaacag aagtaacttt gtctgttatc cctcgtgggt tttcaccata ggcccctaaa
     5941 cagacatcga ttttgtgttc attaccatgt ttggcaaata aattggagta atgtgaatct
     6001 cctgcatgat aaagattccc tcctgatgtt tcgaataaat agtttactgc aatttgatcc
     6061 atctcctgag gcatttttcc ttttaatacc tcttctggat cttcacaagt gaccaacgct
     6121 gtccgatcaa acgcttctaa agctactaca ttgatgtctt tgattttgac tgcatcacct
     6181 ggctttactg taatcgtttt ttcttcagga attccccatt tttgccacgt gcgaaccact
     6241 tcttttggcc caatgaatgt tgcttcagga caattttgat gaactgctgc ggctgtatgg
     6301 atatctagat gatcggaatg gatatgtgta acgattaaag cgtctacatt ttttatcgca
     6361 aaaggatcga ttacgaaagg ctgagtacgt aaattaggct gcatattttg aacgccactc
     6421 atacgcatca tctgatggcc ttttttcatc atgccattcc catgactacg ttttcccgtc
     6481 ccacaccata gatcacataa aatattcgtt ccttcatgac ttttcagcca aattcccgta
     6541 cagcccagcc accacatagc aaaagttcca ggatccacta cttctgtttc gatttcttca
     6601 ttcaagtatg tgccccactc aggaaatgtt tctactaacc acttttcttt tgttacttca
     6661 ttgacagttt tactcatttg cttgctcccc ccatatcgtt gttccaactt acaagtaaat
     6721 attaatgtgt atcagcatcc atgaaaaccc ttttatatga ccgtttgttt tttatatgat
     6781 tttctttcag taatcaatga ttgtttcagt ataataaaga cagatatgaa tgtttaaatg
     6841 aacaactagt caaataaatc catggatcaa gatgtccctc aggaggcaaa agtcatgaaa
     6901 aactcacttg tcaatattga aaaacgtcaa aaagaaattt tggaactgct tcagcagact
     6961 gaacaactat caactttcga attagctgaa tcccttgatg tatctattag tacgatacga
     7021 agagatttaa atttattaga agaaaaaaac gagatcatac gcaattacgg ttattgttct
     7081 tataattaca aaaaccagac tgattttgat caatctggtc ctgaaaggat taaacaagcg
     7141 attgccagag aagctagtaa atacattaac gattatgata cgctgtttat caattctagc
     7201 tcgactgcgt tgaaaacact ggattttctt caatctaacc atttgacagt cattacaaac
     7261 aacgtgaaaa tcaattactc tccgcacaaa gataattgta gttatatttt aactggtgga
     7321 gagatgcgct ttccaaaaga agcactagtg ggcgacatcg cattaaatac gatttcttca
     7381 gtcaatgcag acatttgtat cattggctgt agcggagtcg atgtagtaca tggggtcaca
     7441 acaaagatat taaatgaatc aacgatcaac gaaatgatgg tcaacaagac aatcaaatgt
     7501 aagattttag tcgctgatca tcgaaaaatc ggactgacat caaagttcaa gattgctgat
     7561 atttcagtct ttgattattt gatcactgat aaattttgtt ctgaaaaagt attagaggag
     7621 atcagaaata cggggataaa aaccattcaa gtagattcat tttaagaacc aggacgtaaa
     7681 tatgtaaata ggattatcaa aaaaatgaat aacaaattta tcgataaatg aaaaacacgc
     7741 tacgctcaaa ttgataaagg aatcccaaga tgtatccatc caatcgatac catcataatc
     7801 tattgcttct tttataaccg ttatatgttg cctaaatagg aatcaaacaa tatcgaaaaa
     7861 aggaggtatg acaaaagtca cacctccttt tttcgaataa acggtattca aatcagaaca
     7921 ctgatttcac aaccacttta tttcaggtcg ttttcaatca cctttaccgc gtaatcattt
     7981 ccgttgaaac gcgctatttt ttttgcgtcc atatatcttt ctaaataatc atcttctcct
     8041 aattgcttat ggcaagaagc aatttcataa agcaagtatt ccaatgcata agtcgattta
     8101 tgtttcttat tatactcaat acccttctca cataattgga ttgctttatc ataatcctcg
     8161 attttacggt aataggtggc gatatcataa tatacaatgc agaccatcag attatcaaaa
     8221 tcagtctcac tcaatatttt aagtgtacgc tccacataaa ctttcgcaaa atctaattct
     8281 ccttttgaca agtagatcat tgccacgttt gcagctgctt gtaagtgatg gatatctgtt
     8341 tcggtcgttt gttccaacac ttgattaaaa taaaataatg ctgcatctgg ttttttcgca
     8401 taaagatgat caagcagtcc ttgataaaat aaaatttgat ttaaaagatc agaattactt
     8461 actttttcct gatcgatcgt ttgtaaaatt ttataagatt cttggtattt tgatacagaa
     8521 ttcaatgatt gaatctcatt tatttttttc ctgattttgt cttcttctgt ttctactaat
     8581 atatcggaga tctcgacatt cagctttttg caaagcgcac ttaagatatt gatgcctggc
     8641 gacatattct tgttttcgat attgctgaca gttgcttgtt cacaaatgcc ttctgctaat
     8701 tgtacttgtg ttaacccctt tttctttcgg tattctttta gtaatgctcc atctacttta
     8761 atcaaaacaa tttccttccc tttaaaaaat tatttctcct ttatatcata cacaaaaaaa
     8821 agttctaaac aaccaaaaat gtttagaaca gaataaaaat tttaatttta aaataatatt
     8881 caaattaaac aactggcgta tctgctcctt gatttctttt tgtgttatct gaataattat
     8941 cgtttacagt tataacacat agtgaaaatt gtcatggcga gaaagtctaa gaatattttt
     9001 aaacataact atttctttct tacgtgtgta taatgacgac agcacaccta tcaattaata
     9061 taattatttt atttttttat gttctcatgt ttatttttaa tgacacaact tctcgtattt
     9121 atctggcaca acatataaat cgatcaaaaa attgttgcgt gtttttttga aaattggagt
     9181 ttcaacttga ttacaaaatt taaatttaga agttgtgaaa acagcgagag acagtttaat
     9241 attcaaaata tacataggct actcgaacgc tgtgtttttt agtatactag taagaaacga
     9301 agggatagac tcattccaat aaggaggatt tcatgaagac aattcttttt gctcctgcag
     9361 tattcaattt agctgagaca acacgaatga ttgaggttgc caaagctatg aaagaccgtt
     9421 attactgtgt attctttggc ttttctaaaa cttttgctcc tttgatcaca gaaagtggct
     9481 ttgagtatca tttactagcg ccgatcctta cgaaaaaaca agaagaacaa atcataaaat
     9541 ttgaccagtt gcgtacactt aaaaaccctt ttaccaaaga aatggttgcc tctcgtatca
     9601 ctagtgaaca gagtctgatt gctaagtttg agcctcgact gattgtgaca ggttcgaatg
     9661 tgacaatttt tttatcggcc cgcaataaaa agattccttt agtatatgtt aaaccctatg
     9721 ctttgagtga accttttttt caatcagagc atgcgctgat gccccacgag ttacgtttct
     9781 tcggcccttt aagcaatccc ctttggcact ggacaaaaaa gcgtatagta aatacccgat
     9841 ggatccccaa tgccttcaaa cacgctgcac aaaaagaaat gcttccttta ccaaaaacaa
     9901 gtttacaaat gatggatgct gatctaaatc tcatcacaac gcctaaacta tttttggaag
     9961 aagaacagct gccggaaaat tatcgaatcg ttggtccaat ttttgctaag ctcagtactt
    10021 ccttgcctga agaagtgcta acttttatac agcgaaagca acaagaaaga aaacgtatta
    10081 tctatttcgc aatgggtagt tcaggcaatc cgcgtatgtt acgcaaaatt ttgttcttct
    10141 tacaaaaaca accggatcta gcggttattg cacctattga ctatctgctt gatgacattc
    10201 aatctgactc agaagatctt ttaataacag aatatctgtc tagtcatctt ttgcatgaat
    10261 tcattgattt ttcttttatc cacggcgggg aagggaccgt ccaaactgcc tgtgaatccg
    10321 gcaaaccgtt tatcggtgtg gggatgcaat ttgagcaaag ctgtaatatt gactattgtg
    10381 taaaatacgg aaacgcaatc gccctcacaa aaccagtgac tgaaagaaaa ctgcagattg
    10441 ctctgcgcga agtttcttca cccaagattc gtcagcaggc acgcacgctg caagccgttt
    10501 ttccaataaa tggtccacaa caagcaattc aagaaatcga aaccttcctg agcaaccacc
    10561 caatatagtt tcaagtagaa aaagcttttt taatagagac tcatcagtaa tttcgctatt
    10621 cgagacccag acagtgaaaa tacaaaaaaa gtaaatagtg gattgaaaag ttagagttac
    10681 ttaaattacg gtgtctaaaa aacacaaaat catccttaaa ataaaagatc tgacgctatc
    10741 caaatgagca taggtagcgt ctgccattta ttcttagtca aattctttga cacctttctc
    10801 tggttggatc atctcaacgt ttcttcaaaa gaaagaccat aataattagg caaatacatt
    10861 ttcttaaaat gtgtcatata gatctagaag catcgctttg tagagatttg tgccgctagt
    10921 cccctcaaca attactaaat caaatgtagg atttaccact ttatctttga taatcaacct
    10981 ccctcttctg ttgagacaat gatctcgcaa tccgatgcat actcctatat aaactgccag
    11041 caatccccac atattgagta gatcatgtca gaatgttcta tgtcgtcagc aacgatacac
    11101 taaaagtcac gttcaccatg actgattgct attcccagca caattatttc tatacacgtt
    11161 tcatgatttg aataaacact cacatcatac atattaccat tcatacacca tactgcagcc
    11221 cctactatat ggttgtaagc tcccaaatct atatttgact tgatcacttt ggtagtagat
    11281 ctactaattc taaatccatt gctgataaaa tttccatttt ttcctccttc tttctgccaa
    11341 aacagttaaa acaataataa tctttatgta aaaaagggct gtgacaaaag ccttgtcaca
    11401 gtcccatatt ccgaataaac ggtggtcaaa agcttgcatc tgcggtaata agttcaacca
    11461 aacgaaaaat aagccaacat ttcacaaaaa tttaagaagc aattttcgta aatttcttct
    11521 tatttcttga agctaaccac ttctgttaca accactttta gaacatttaa agcttcgatg
    11581 tgacaaacca tctatgctgc tcgatcataa aattgatagc aaaaattcca caaagtacat
    11641 atgtccccca tgaaaacaaa acattcatat gcggtacaat gatctttgga agaatcacga
    11701 tcaaaaatga tgccagtgcc caaatccatc gattgatttg aatatgatat gtagctagca
    11761 tattcaatat aaggtgacta gccgttaata aaaagataaa aacaaaaaat aaaatcagcc
    11821 aatacattgt ttcccacctt ctaaaaacca aacttagaca gtctggactt cgttttctcc
    11881 acgactttgt tcatctgaca gcatcttttt atcataagct tttacaaatg gataatagat
    11941 aataaatgct atcaatgtgt tgatcatgat caagataaca acacgccagt catttcctgc
    12001 tgaaagataa ccgccgattg gagctggtaa tgtccatgtt ggcaaaacag taaatctatt
    12061 gaccatttct aatttgactg caaaataact gataatagtc aaaactaaag gaactaagtt
    12121 gaacgggatg aacatgtaag gattcaacat cataggagct ccaaaaatta tgggctcatt
    12181 gatattgaag ataccaggaa taatagaaaa acgaccaacc tctttcaaga attttgattt
    12241 acagaagaat ataaacaaaa cagataccac gatcgttgct cctgcaccac caattgttac
    12301 cgtccattga taaaactgtt caggagcgat atagggtaat tgggtggcag ctgtaccatc
    12361 tgccaatgcc tttgcattct catccaacat gatcagccac attggacgaa caacagcccc
    12421 tacaatactc atgccatgga taccaaatga ccacaacaaa tggatcaaaa acattggtag
    12481 taaaacacca agcaaattat tccctaaaat attggttaaa ggctcgaata acgataataa
    12541 ggcagcgttt acatcaaagc ccgcgacgct tcgaatcagc caagtaattg ttaaaatgac
    12601 tgcacctgga atcaatgcct caaaactacg ggcaacagca ggtggcactt gttctggcat
    12661 tttgatagta atatttcgat ttttaaagaa acgatatact tccactgcaa aaatcataga
    12721 taagatcccc gcaaacatcc cagatgctcc caaagaactc ataggtaaga cccagccaag
    12781 aggtgcctct tcaggagtta aaacgtttac aggaacttgt aacataaaga acgttcctaa
    12841 agataatata ccacctgtca cactatctaa tccatatgat ttagctagat ccgaacccat
    12901 aacaaaagtt gcataaatag ccatcaatcc catcgtcatc ctaaagggta aaacaatcgt
    12961 tgccgaataa ggggcaataa attcattcca tccaggaaac ggtaaattga taaagaccat
    13021 aaaaaatgtc ccaatcaaaa tcaatgacaa tgttgaaaca accccattac gaattgctaa
    13081 taaatgacgc tgtgtaccaa tttttgccat tggtgggaca agtttctctt cgacccattt
    13141 catcagcttt tccactaaaa ccactccttt tcaataaaat atgaaccatt cattgcatga
    13201 caacgcaaag aatgttcata actaattata taa
//
LOCUS       DAYYGO010000077        13212 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000077 DAYYGO010000000
VERSION     DAYYGO010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.079"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            147..1016
                     /locus_tag="ACHA5T_001931"
     CDS             147..1016
                     /locus_tag="ACHA5T_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8887496.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1225..2250
                     /locus_tag="ACHA5T_001932"
     CDS             1225..2250
                     /locus_tag="ACHA5T_001932"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM8887497.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2289..3239
                     /gene="manA"
                     /locus_tag="ACHA5T_001933"
     CDS             2289..3239
                     /gene="manA"
                     /locus_tag="ACHA5T_001933"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFM8887498.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            3333..5036
                     /locus_tag="ACHA5T_001934"
     CDS             3333..5036
                     /locus_tag="ACHA5T_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8887499.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5038..7071
                     /locus_tag="ACHA5T_001935"
     CDS             5038..7071
                     /locus_tag="ACHA5T_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFM8887500.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7082..7522
                     /locus_tag="ACHA5T_001936"
     CDS             7082..7522
                     /locus_tag="ACHA5T_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8887501.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(7722..8849)
                     /locus_tag="ACHA5T_001937"
     CDS             complement(7722..8849)
                     /locus_tag="ACHA5T_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8887502.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8852..9529)
                     /locus_tag="ACHA5T_001938"
     CDS             complement(8852..9529)
                     /locus_tag="ACHA5T_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8887503.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9793..11559
                     /locus_tag="ACHA5T_001939"
     CDS             9793..11559
                     /locus_tag="ACHA5T_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFM8887504.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11574..12011
                     /locus_tag="ACHA5T_001940"
     CDS             11574..12011
                     /locus_tag="ACHA5T_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8887505.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12024..13175
                     /locus_tag="ACHA5T_001941"
     CDS             12024..13175
                     /locus_tag="ACHA5T_001941"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFM8887506.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT         4488 a         2293 c         2731 g         3700 t
ORIGIN      
        1 tttataagag tttttactca ttaatttaca aaaaacaagc aaaatgcgaa ttttctcaca
       61 ttttttttaa caaaatcaaa gttattgctt gaaagaagaa gaatccatcg ttaaaataaa
      121 gcttgacgta gaggggagca agaaatatga aagattttca attgggggct gccacctatt
      181 taaaaagtgt agccattcgg gtgaaagacc gagataaaat gattgatttt tacaagaacg
      241 ttgttggctt tgatttgaaa agagaagaaa acgaattagc cattttagga acagaagaag
      301 ctggaagtga aatgttgctg ctggaagaaa gtcctagagc gaatgatcac atgggggtga
      361 tcaaaaagct gaaccgtttt tcattgatta tccctagcac agaagaaatg gcagatatcc
      421 tttgtcgtat ccgtaaaatg aattatccaa tcaaagaagc tttagaagat catggacgta
      481 tggggatttt gctcgtagac ccagaagaga atgaattgga aatttaccat gaagagaata
      541 agaacgacca aagagaaatt ccagaaacaa tggatcaaga agcattatta agaaaatctg
      601 aagaaaaatt cagtaaacta tcaacaggtt cttattttga aaaaatccat ttaaatgtta
      661 ctaacttaac aaaagaacgc caatttctta atcatgtact tggtttgaag gtacataatg
      721 aagaagaggg catccatgta ttgaatgaag gagatttcca tgttggtttg acagaagcta
      781 gcggaggcgc aattgatttg cctacagatg aagtgattgg acttgatttt ctgaaattca
      841 tgatcgatac tgaaagcatg gaaggactga ttgctcactt gcagcaacta tcagttgatt
      901 tcttcttaga caaaaagcac acagtactca caatctatga tgctatagga atcgaatggt
      961 ggttcgtcag tgagccaaaa gaaaagaata aaactgacat tcgatgcgac gcttaacttt
     1021 ttctttctta acgattgtcg catgtctcgt cattttatga acggtcatac tatcgcttat
     1081 tctagagata gaaagagaaa atagtatacc gggggaaact gcgcggtttt tcgcgcagtt
     1141 tttttgtgta aaaaaggttg attcctcata gattaagctt taagcttgga ctgtaagaat
     1201 ctgatagaaa agcaggagaa aaagatgtat ccacacacga aacagcttat tttgaaaaag
     1261 atggaagaag gattttatcc aggtgtagca gtcgccttca taaaagaaaa tcaagtagaa
     1321 acatttacag ccggctttgc ccaagtcgtt cctgaaaagc agctgatgac agaagatctg
     1381 ttgtttgatg cagcctcact gacgaaagtc gttgcaacca cttcattagt tttacgtcta
     1441 ttagaagatg gaaagataga actcgatcaa ccgcttcata aatatcttcc tgaatttgaa
     1501 aatcagacaa tcactttgcg tcatttactg acacatactt ctgatatcaa aacatggata
     1561 ccaaacagag ataagctatc tcaggaagaa ttgatcgccg cttatttgaa gctagaacca
     1621 ggtgagcaac taggaaaaga agtaaaatat acagatgccg gtacgattct tcttgggttg
     1681 atgctagagg aaatttacca agaaactgca gtggaaatct ttcaagaaca agtattggaa
     1741 ccattaggaa tgatgaacag cttatttctt ccttttcctt ccaaaaaaat cgttcccaca
     1801 gaacaactgc cttcgggtga ggttttgcgt ggacaaactc atgatccgaa agcaagagtt
     1861 ttggctgaac atgcaggtaa cgcgggtctc tttattgatt tgaaagacag cattactttt
     1921 gtccaaatgt atttgaacaa gggaaaaatc cgtaatggtc gttttttaga agaagagacg
     1981 attgattctt tactgcaaga tcaaacaccg aaaaaaaatg gctatcgttc tttaggatgg
     2041 gacttaaaaa aggatgtaga agacggtcat gcgattcttt ttcatactgg ttatacaggt
     2101 acgtttttct tgattgatat aaaaacacaa gaagcgttga tatttttgtc aaatcgcgta
     2161 catcctgtcg atcatcgtgc ggagtatctc atatgtcgag atgaaattgt tgaaaacttt
     2221 ttgaaagaga aagcggtatt accgaaataa tgctataata gctatgaaaa atgagaggag
     2281 tttgaattat gcaggaacct atgtttttaa aaccagtttt tcaagaaaaa atttggggag
     2341 gaagtcgttt acgttcagtc tttggttttg atatccctaa cgataaaatc ggagaagatt
     2401 gggcgatcag tgcgcatcct catggtgtca gtgtagtaga aaatggcgaa ttcaaaggga
     2461 aaagattaga tgaactatgg tcagaacaca aggaattgtt tggccatcct tcagaaccag
     2521 tgtttccatt attgatcaag atcttagacg cagaagatga tctatctgta caagtacatc
     2581 cagatgatgc ctatgggatg aaacatgaag gtgaattagg aaaaactgaa tgctggtata
     2641 tcatcgatgc tgaacctggt gcagaaatca tctatggtca tcatgcaaag acaagagaag
     2701 aactagctga gatgatcaaa gatgggcgct gggatgacct tttgaagaaa gtaccagtta
     2761 aaaaaggtga tttcttttat gtaccaagcg gaacgatcca tgctattggt aaaggaatca
     2821 tgattttaga aacacagcaa agttctgaca cgacttaccg cgtgtatgac tacgacagaa
     2881 aagatgcgca aggacaaaca agagagctgc atatccaaca atcgatcgat gtaacaactg
     2941 tccctgcgaa aacaccagaa ttacaaatca aagaagtccg aaaagggaat tcatcgatcg
     3001 tcacttattt agaaactgag tttttcaatg tatatgagtg ggatattaaa ggaatcacat
     3061 ctttcaaaaa acaagcccct tatacattga tgacggtaat cgaaggagca ggagaccttg
     3121 tcgttgacgg aaaaacatat ccattagaaa aaggaacaag ctgtattatt ccaaatggcg
     3181 tttctgagtg ggcggtccaa ggtgaactag caatcattgc gactgaacca ggcaaatagt
     3241 tcccagatag ctgaaagcag cagacggttt ttcgtttgct gctttttcta cataaaatcc
     3301 gtccatcaat aaatagaagt atggtgaaaa agatggaata caaatggaaa gcaaacaaca
     3361 gcagtcgcgt gtctatgaaa caactgagaa aagctggaca tcacttaaat gaaatgatca
     3421 tgccaaatct aagtctgttt atcgcttggg gaatcttgtc attagcaaca aaaggtgtgt
     3481 ctggcgattt gcacaccaca ttagaagagg tatgccattg gatgatccag ctgcttttac
     3541 ccatcttgat cagttattca ggcgggaaat cggtaggggg acaaaaaggg gcagtcgccg
     3601 gtgcaattgc ttctttaggg ttgatcgctc gttccaatgc tccacaaatc gtaggagcga
     3661 tgattatcgg gccgcttgcc ggcatcgtat atgaacaatt tgtttctaga ttccacgtga
     3721 aattcaaggc tggatatgaa atgcttgtca gcaacctact agtaggactg actggtagcg
     3781 ttatttgtat aattggaatc gtttttattg aaccagtttt aaatagtgtt cacttgttgc
     3841 tcgcctccgt tgtgtcttgg ctagtcacaa tgaatctgtt accacttgtc agtctgatct
     3901 tagaacctct aaaagtacta tttttcaata atgcaatcaa tcatggtgta ttgacacctt
     3961 taggaatcga attctcacaa acagtcgggt actcttcctt atttttgatg gaggcaaatc
     4021 ctggacctgg tttagggatc ttactttcta tcctttgttt tgcagaaaaa caggaaaaag
     4081 taaatgcgag tggagcattg atgatccatg cgattggcgg gattcacgaa atctattttc
     4141 catttgtctt gctgcgaccg tatctatttt tagctgtaat ggctggtggg gcaagcggca
     4201 cgttgatatt tcaatggtta gatacgggat tagctgcacc tgtatcgcct gggtcactac
     4261 tcgtgatttt tgccaatgcg ccttatccac aactagctgg ggtaagtata gggatagcaa
     4321 tcagtacatt tgtgaccttc atttgtgcag cgatactatt gaaattacca aataaagaaa
     4381 aagagaacaa aacggtcaaa gaaaggggag aaatcttgga accatctatc attcattccg
     4441 tcatttttgc ttgcgactca ggtatgggat caagtgctat gggagccgcc ctgctgaaaa
     4501 agaaattagc agcagaaaaa atcgatcttt ctgtcggata cagctctatc tatgaattga
     4561 ataaccaagt aaatcagctg gttatcgtgc aacatgaact agcgggtctt gcaagagaaa
     4621 aagtacctca aagtcaaatc tatgtactgg atcacttttt ggaaacagat atcttgctgt
     4681 cacgaataaa agaagcgatc caatttccaa agcgggaaga aaaaaaagag atagcagata
     4741 tcacagaaca aaaaaagtcg cctgctaata ttgtattttt gtataaaaac aacagacgcg
     4801 gctcacaaac tatgggcatg acgattttaa gtcaattagc aaaaaaagcg aaaaaacagt
     4861 ttgttatctc aaaagaatca atagaaaaaa tcaaaatcca agaagaaacg atttatatcg
     4921 cacaaagaga actagctgta actgaacagc tagaagaaaa gatccctcgg ctagttctag
     4981 ttgaacattg ggtagctacg aatgaatacg aaaaactcgt gaagggagaa atataagatg
     5041 tatttatcgc ccagagaaaa acaattatta gcagaatttc tcagtagccc gtcacctgta
     5101 tctatccaaa aaatgatgaa tttattgaag gtcagtaaac ggactattta ccgcgaatta
     5161 gataatcttg aagtctcatt gaaatcggta aatgcttctt tgaaaaaagc agctagaggc
     5221 tcatttgtca ttgaggcatc agaagaagta ttagagcaat taaaagaaaa tacagatggg
     5281 aaaagtaatg acttatccac tgctaaacga caacacgcga ttttagccga gttgttactg
     5341 acgacagagc cgttgagtct tgctcatttc atggaatcat atcgcatcag taataccact
     5401 ttttatgcag atatcaaaca gttggaagaa agtattcgcc aactgccttt ggaaatcatt
     5461 cggaaccagg gatacgagat cgaaggaccg gaaaaatacc gccggcttct gacagcgaat
     5521 gtattagaat tagaaatcaa tgaatatgaa ctttttcata gtatttcttt cgatgcatca
     5581 ctgaattatt tttttcgatt cgttgatccg caacatctat ctcttgcacg caaagtaatc
     5641 ggggaagaat tgatccaaaa aaagaccaat ctaagtgata gaaagcttga acatttggtt
     5701 ttgatgctga cattgacgat ggagcgtgtg actaaaaatc atttactgac aaatgagaca
     5761 tataatagtc tggcaaacaa agaattacta cagacagcta aacgattgtt ttcacggatc
     5821 gcttctgaga caaaacaact ttatccagta aatgagatcg tcttttttgc aggattatta
     5881 aatgacttca cgaactcatt tgatcagaat ttctttgaag agacgttcga tacacagctt
     5941 gcttatttag taaaacaact gattcaagaa gtaagtgaag agacgaatgt ccatttttat
     6001 gaagatgaaa ctttgtacaa aatgctgttg actcatttat ctggtgtgtt ttcaagagcc
     6061 gttttacaag aagagacact gaccaatccg attttagaaa aaatcatggt tcaatacgag
     6121 gaagttgcga atgcaatccg taaagcagta gagcggattt ttcaagaaaa aaaattatcc
     6181 gaagaagaga tcgcttatat ggtccttcat tttgcgaatt cattggaacg aagtccgaaa
     6241 gaaagcaata tcagtattgc aggattttcg ccaagtggat tagcttctac tagcatgttg
     6301 gaactacgat tgaaaaggta ttttcccttc atccaacaaa ttcatttcta tcggattgct
     6361 gatttaaaaa aaatcgattt acaagaagaa tatgatgttg ttatttctac atctatttta
     6421 ccaggatacg gtggaaaata tattttagtc tccccgctac tgctagaaga tgaagtcaag
     6481 cagctaaaag aagctttttc gcatattgag aagaaaaaaa ggcaaaaaat tacgtataac
     6541 agcaataacg agtggatgat agaagatact tatgaggaaa caatgcattt catggatcaa
     6601 atcaatcaat tactcggtcg tttttttatc caagaactgg ataatcctga gacgatttcc
     6661 gaaacgattc gcagcttatt tccttatttt cctgaaggac tagtaacgga tgaagaggtg
     6721 gttcatcgcc ggctgcttaa gcgatatgaa caagcaccta tcggtattcc tcatacaaat
     6781 atggggttat tccatacatc cagctcattg atttctaaac cgatcttttg tattttcaat
     6841 ttgaaacatc cgttgaccat tgaagggatg gatagacagc cagtcgattt gtcccgtatg
     6901 ttagttatgc tggcgccttc gccaatccaa gaaaaagacg ggattctttt aggaaagatc
     6961 agtggagcaa tcatcatgaa tgacttgaac accgagatct tccgctcagg aaataaagaa
     7021 atcgtttatc agttgttagc gaaaatcctg attgaagaaa taaagaaatg aaggtgaaaa
     7081 gatgatacag ctaccagcag aaaaaatatt gctcgatcaa tcctatcaaa attggaaaga
     7141 agcgttgacc gagatcggcc agtggatgac ccaacaaggg ggaatcaaac cggcatatat
     7201 ccaatcaatg atcgaaagac aagaaaaagc gagtgtctat atcgggaatt tcgtagcctt
     7261 gccacatggg actgacaaag atgaagaagt agtagaagaa ggaatctatt tagtccaagt
     7321 gcctgatggt gtggactttg gaacaaaaga ggaaccaaaa atcgcgacac tcttatttgt
     7381 agtggcttta aaaaaagaac atcagttagc ggtgctccaa gatttagcct ttttctgttc
     7441 agacatcgac caagtaatgg cgctcagtga tgcgaaaacc attgaagaag tccaaatgat
     7501 ttttgaacat ggacatatat aaccaatgtt cacagtaaaa aaagctgcct tgcatcaagt
     7561 aataacttca accaagataa aaataagagg agctattttt gttgggaggt tcttattact
     7621 gcggatgcaa gcttttgaaa actatttatt ctagaaaacc gtttatttga aataggggac
     7681 ggtgacaagg tttttgtcac cgcccctatt atttttgtgt tttaatagct cttttttata
     7741 tagatagtgc ccatacctat gaataataaa acgatactcc atataccaag tgtcaagata
     7801 ccattcatga aagtaacctg ttctgaagct agtggcgaga aaatatctcc tcctatgatc
     7861 acattttgaa aatcaacata agaagttggc aagtaatgac tgattgattc tatccatcca
     7921 atcgaagaaa acaagcgata ctctgctaag aaaagacaaa gaagtgtacc taaaagattg
     7981 actagagaat gttcaaataa agcagataga agaaaactga ttgtacttaa aaagagcacc
     8041 cacaacaaca ggaacagaaa gctctggaaa agatactcat acatcggaag gattcgtatc
     8101 tcctgaccaa tgatcgttcc tacaggataa cggaagtctc ccaaaccatt tttgattatt
     8161 actagtatag agacgatcaa aaatggcatc acgagactta gcatagctgc accataataa
     8221 atggtaaaac ggatgctatg tttctttacc atactatccg gtaatagatt gactagagag
     8281 attacctgct ttcttttttc ataagagatg atggaagaaa gaaacaaggc ggttatacat
     8341 aaaatcaaaa aactagaaat cattcctgaa aaaatatttt cgtagtaatt tgctaaaggg
     8401 agatgcgctg gcaagtcaaa aatagaatat ctttggatct gatgttcttt catataacgg
     8461 agcaactcga cacgtttttg ttgttcaatg attggaattc cttgtctttg accagaaatc
     8521 agtcgatcca gtatatcttt ttcataatga taagttgctt cgaccatttg ctggacattc
     8581 cctgtgtttt ttgcatgtaa gattgcttcg atcagtttgt tgcttttctc taaactttcc
     8641 acaaattctt tctcattctc atcttctttc aacgtttcaa tcaaacgttg catctcttca
     8701 tagttatgat tcagttcatc gaattctgta taagcaagtt tatgatcaaa aaaaagtggg
     8761 aatgcaaaga gcacgaccaa caatagagga aataaaaagt ttactttgtt ttttagtaag
     8821 agattgcgtt caaatgtaag taaagacata gttacacctc atataattta cgataaatag
     8881 ctaaaatctc ttccatattt gaggattctg tgacgatttc tccatttttt agaaaacatg
     8941 ctttagaaca aatcgattgg atattcgcga tagcgtggga agaaagaaga atcgtcttcc
     9001 cctctttctg ccattgtctg atgaggctat tcaagatatc aatacttcct gggtctaatc
     9061 cgttcaacgg ttcatcgaat aacatgacag atgaatcact gatcaagtaa gcagcaagca
     9121 agacatgttg tttcattcct agagacattt ttccaatcgg caaagtaagg tactcagtca
     9181 tcttcaatcg ttggaaaaca gtttggcatt ctactttgct ctgccaatag tatttgatca
     9241 aagtcaaata atcaacagcc gatagatagt cggataactg attcgttgat tctagaaaaa
     9301 agaaagaccg gcgataatct gtataagcag taggagagat gttgtgaagg gacaattgat
     9361 cataagttgt tggcagcagt tgggcaatgc cattcagcaa agttgttttg ccaaatccgt
     9421 tcggggcaat caaagcagct acttctcctg attctactaa aagattcaat tgtcgaatca
     9481 cacgtttttt accacgtttc atgttcatgt ttttgatcag taatgtcata gataggcctc
     9541 caatataaaa tttaataaca taatagaatg gaaaacatct aaattaaatg ctaacaaaag
     9601 ttattgtttg catattataa aaaaattaaa ataacagttt tatttaaaat tgaagttttc
     9661 tgataccgaa atctttttat tttgctgaca tactcaaacc tttggcacaa agtttcgtgc
     9721 caaagtacga ctatttctct ttttagtaag cggatacaat tagagtatca aaaggagaga
     9781 gggagagaca aaatggaacg gacgattccc aaaaacaaca tttcgctaaa agcgagggta
     9841 caaaaattag gcagtacttt atcaagcatg gtcatgccaa atattggagc actgattgcc
     9901 tggggtgtat taacggctct atttatccca gatggttatt taccaaatga ggctttggct
     9961 accatggtaa gcccaatgct aacttatctg attcctttat tgatcggtta tactggcgga
    10021 aaagtaattg ctggtgaccg cggatcagtt gttggggcaa ttgcaacgat gggtgtgatt
    10081 gtcggaacag atattccaat gatgctaggc gcgatgatca tgggacctct tggtggatat
    10141 gccatcaaga aatttgatca gctattccaa aaacggatca aatccggttt tgaaatgcta
    10201 gtcaataatt tttcagcagg tcttatcggt tttggattag cgttattagg tttttctgcg
    10261 attggtccag ttgtcgatgc attgactcaa gcaatggcaa aaggtgtaga gatcatttta
    10321 tctgcccatt tgattccatt gacaagtatt tttattgaac cagcaaaaat cttatttttg
    10381 aataatgcaa tcaatcatgg gattttgaca ccattgggaa ctgaacaagt agcagaggct
    10441 ggaaaatcta ttctattctt gcttgaagca aatcctggtc caggacttgg cgtattactt
    10501 gcttttactt tgtttggcaa aggagcagca aaatcttcag caccaggagc catgatcatt
    10561 cactttttag gcgggatcca tgaaatctat tttccttacg ttatgatgaa accgttatta
    10621 ttcttatcag ttataggcgg aggaatgtca ggaagtttca ttttccaatt gatggatgct
    10681 ggactaagag cgccagcttc tccagggtcg atcattgcga ttctagcgat gacaccgttc
    10741 aattcttacc ttcctgttat tttgggcgtg attgttgccg cagctgtttc gtttggtatt
    10801 tctgcagtaa ttttgaaagc ggatgcgaaa gatacaaatg aagacttcga aaaatctgtt
    10861 caagagacaa aggcagctaa gcaagcttcc aaaggtgccc aaggtattcc tcaaggagct
    10921 acattctctg gcgtacaaaa aatcattttt gcttgtgatg caggaatggg atcaagtgcg
    10981 atgggcgctt cgatcttgag aaaaaaagta caggaagcag acttgcctca aacggtgacc
    11041 aatcaagcaa tcagccaact gacagatgac tcgaatacat tgatcgttac tcaggtcgaa
    11101 ctacaagaaa gagcaaaaca aaaagcaccg agcgcgcgtt ttgtctcagt agaaaacttt
    11161 ttgaattcac ctcgatatga tgaaatcatt caagaattaa gcagcaagca gaaaacatca
    11221 gtttctgaaa ttgagaatga gccagcagaa gagtttcttt cagcaaacat caaacaaatc
    11281 aaagagattc tacttgtcca cgatgatcgt tccggatcag caacaatggg gatgaccgtg
    11341 ctcaaagata tattagacaa gaaccattta actatcccgt taagaaaagt caatatccaa
    11401 gatttaaaag atgaacctga ttcactgatc gtagccaaag aaagtttagc ggaaaaagta
    11461 cagatggctg cacctcaatc cattcattta tcagtcaaca gcatggtcaa tccgcaaaaa
    11521 tacgaatcga ttgcacaata tttgaaacag acagcttaag ggaggaaaac agcatgcaaa
    11581 gtttgccatt cgacatgatc gagctcaatc gatcttttaa aaccaaagaa gaagcgatcc
    11641 gctattgcgg tcagatgctt gtagaagcag ggtgcgttga tgaggaatat atcgatgcca
    11701 tggtagatag agacaaaatg ttatctgttt atatggggaa ttttatcgcg attcctcatg
    11761 gaacagatga agcaaaaaaa tatgtgaaaa aatccggcat ctgtgtcgtt caagtaccag
    11821 atggtgtaga ttttggtacc gaagaagaag aaaagattgc gactgttttg ttcggtattg
    11881 caggagtcgg agatgagcat ttgcaacttg tacagcaaat cgcactttat tgcagtgata
    11941 tggacaatgt tgtccagcta gcagacgcgc tcagcaaaga agaagtaacg gaaaatctag
    12001 caattgctta aggagagaga aaaatgagag cagtacattt tggtgcagga aatatcggtc
    12061 gaggatttat tggagaggtt ttggcagcta atggatttga gattactttc gtagatgtga
    12121 atcacacgat cattgatgct ttaaatgaaa gaaaagaata cgaaatcgaa ttagcagatg
    12181 aatcaaaaga aaaaatcact gtaaaaaatg tcaaaggaat caacaatcaa acaaatccag
    12241 aaaaagtaat ccaagaaatc gctgaagcag atttagttac taccgccatt ggcccgaata
    12301 tcttaccttt tattgctgag ttgattgcaa aaggtatcca agaaagagaa aaggaaggaa
    12361 gcacaacatc tttagatatc attgcctgtg aaaatatgat tggcggttca gcatttctag
    12421 aaaaagaagt gtataaatat cttccggaaa catcatttac tgacaaatat atcggctttc
    12481 caaatgcagc agtcgatcga atcgtcccat tacaacacca tgaagatcct ttattcgtcc
    12541 aagtggaacc tttcaaagaa tgggtcattg acgatagcca aagaaaaaat aaaaaaatcc
    12601 aattaaaagg ggttttatat gtagatgatc tagaaccata tatcgaacgc aaactattta
    12661 gtgtcaatac tggtcatgcg actgtcgctt atacaggtgc cttgttaggt taccagacca
    12721 ttgatgaagc aatgcaagat gcgttagttg tggcacaatt aaaatcagtt ttgcaggaaa
    12781 caggaagttt gctgattgcc aaatgggggt ttgatgcaga gcagcatcat gcgtatattg
    12841 aaaaaatcat tcatcgcttc caaaacaaaa atatctctga tgcgatcaca cgtgtcgctc
    12901 ggacaccatt acgtaagtta ggttatcaag aaagatttac acgtccagtg agagaactac
    12961 aagaacataa cttgacttgc ccacatttga cggcaacaat ggggattatt tttaattact
    13021 atgatcctga agatgaacaa agccgtcaat tgcatgaaat gaagatccat gaaaacttgg
    13081 aacaattgat tcaagaagtc acaggaatca atgatccgaa aacaatcggg aatatcaagc
    13141 aaaacgtgaa ccgttatgcc aagcaagtcg cttaaaatag gatacgaaaa aggggaatgt
    13201 gacgaaattt tt
//
LOCUS       DAYYGO010000078        13085 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000078 DAYYGO010000000
VERSION     DAYYGO010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.080"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(78..2198)
                     /locus_tag="ACHA5T_001942"
     CDS             complement(78..2198)
                     /locus_tag="ACHA5T_001942"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8887507.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2428..3606
                     /locus_tag="ACHA5T_001943"
     CDS             2428..3606
                     /locus_tag="ACHA5T_001943"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFM8887508.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3622..4380
                     /gene="aroD"
                     /locus_tag="ACHA5T_001944"
     CDS             3622..4380
                     /gene="aroD"
                     /locus_tag="ACHA5T_001944"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFM8887509.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4403..4888
                     /gene="ybaK"
                     /locus_tag="ACHA5T_001945"
     CDS             4403..4888
                     /gene="ybaK"
                     /locus_tag="ACHA5T_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFM8887510.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4959..6212)
                     /locus_tag="ACHA5T_001946"
     CDS             complement(4959..6212)
                     /locus_tag="ACHA5T_001946"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8887511.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6328..7677
                     /gene="gdhA"
                     /locus_tag="ACHA5T_001947"
     CDS             6328..7677
                     /gene="gdhA"
                     /locus_tag="ACHA5T_001947"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFM8887512.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7789..9135
                     /locus_tag="ACHA5T_001948"
     CDS             7789..9135
                     /locus_tag="ACHA5T_001948"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFM8887513.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9443..9829)
                     /locus_tag="ACHA5T_001949"
     CDS             complement(9443..9829)
                     /locus_tag="ACHA5T_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8887514.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(10097..10246)
                     /locus_tag="ACHA5T_001950"
     CDS             complement(10097..10246)
                     /locus_tag="ACHA5T_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887515.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            10708..10920
                     /locus_tag="ACHA5T_001951"
     CDS             10708..10920
                     /locus_tag="ACHA5T_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887516.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            complement(11198..12997)
                     /locus_tag="ACHA5T_001952"
     CDS             complement(11198..12997)
                     /locus_tag="ACHA5T_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFM8887517.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRSFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT         4291 a         2354 c         2577 g         3863 t
ORIGIN      
        1 tattctaaga tttctcttta ccaacatcat ttgacaaaga gcctaatttt gtcgctgctc
       61 cttttaaatt tattttatca ttcggaagaa cttgtggtcg ttccgtgaag ttcttggtac
      121 gtttgcgttt tataaagatc aacagttgat tgtcccttgt gttcactaaa gacactagtt
      181 gacttgtcac caagagattt ttcagcttct tttaatttct ctaagccatt cttgtagtca
      241 taatcactgg tatcaattgg gtctaatcca ctattcgtat agaaccgaag aagatcacca
      301 ttattgattt ggtcagaaac agctaactgt ttatcaactt tttctttcag tgaatcaatt
      361 tcattctgta cttcctcagt aggttcagta atagcttcac cagtttcgtt actatagatc
      421 gtaccaccgt agtaagtata ttttggtgta acaaaatcac catcacggaa agaaacaagc
      481 tgttcatgat ctttcgagaa tagatcttgt cctaactgaa tataattttt tgtatctaca
      541 cctagtaagt gaagtaatgt aggcaaggca tcaacttgtc cgccgtaagt gtgattgatt
      601 ccgccctgtg tttgtccagg gatatggatc atataaggga cacgctgcat ttgtgcattg
      661 tcgtagtttg tccaagtaga acttgttttt ccaactaatt cagctagatt tttatttctt
      721 gaattcgaga caccgtaatg gtcaccgtaa aggacgatga ctgaattatc atacaagccg
      781 ctttctttca aataattgaa gaattcttgt acggcggtat ctaaataatt ggctgtcgca
      841 aagtaaccgt tgatcgtttc atcatttgta ttggctaacg ggaatcctgc ttcgtcattg
      901 gtgaattttg aataaggata atggttagat acagcaatga attttgaata aaatggttgt
      961 tgcagacgtt ccaagtattg tacggattgt tggaaaaatg gtttatcatg caacccgtat
     1021 tggaacgaat tgtcatcatt aacatcataa taagaagaat caaagaaata atcatatcct
     1081 aaattcttgt atgtctcgtt tcgattccag aatgtaccag cgttcccatg gaataccgca
     1141 ctagtgtaac cttgtgtttg acttaaaata tcaggtgcag cttggaaagt attttttccg
     1201 cctaattgtg tgaacaagga gccttgattc aagccaaaca atgaattttc catcagcgtt
     1261 tccgcatcgc ttgtttttcc tgcttttact tgatggaaaa agttgtcaaa actgaacgtg
     1321 ctgttgctgt ggtaaagact attgatgaaa ggtgtaactt caagctggag accattctca
     1381 tctgtcaatt tataatcgat caggaattgc tggaagcttt ctaaatggat ataaatcaca
     1441 ttttttcctt tagcgagccc gaacaaatca tcatttggtg ctgcataatg ttcttttaca
     1501 taattttcta cttctttcat atcattggga cttgcttctg cccgcacttg gtttgtctgg
     1561 taagtttgaa caccgtcata aaccatgaaa gcattcaagc caagatattt gaccaaataa
     1621 tcacgagaga atgttcgagt cagcaattct ggacgatcag cttccgccat aaaaagattt
     1681 cctgagaaaa tcataactgc taatgtagag atcgcaaaag ccattcttgc tcggatcggg
     1741 cgagggtcca acttgatttt ttttgcaatc agcagaacag tcaagataat aaaatcgatc
     1801 caatagatca catcatatgg gcgaaacagt cgaatggcac tttctcccag accactcgct
     1861 actttcccag cacctaaaat cgtgttgatc gtaatgaagt ccgtgaattc tcgataataa
     1921 gtgacattcg aaaaaacaag caatgtcaaa agaaaataaa tgaccatcat cgtgatataa
     1981 gcaactctag ggcgtctgat ataaagagca attccaagta ataacaatgt agttgcaata
     2041 gggttgataa acaagataaa gtattgaatg ccactttcga ttccgagatg gaaatcaact
     2101 aaataggcaa aaatcgtttt caaccaaagt aaaacaacta gtaaagaaaa aaatccgagc
     2161 ctcttattca aaaaggcagg tgtttttata catttcaaga taatcttcct ttctgcactt
     2221 cttaatcaca gaagtgtcat gaaaccatgt tacacagctc acttattctc atttatttta
     2281 ctagtatgac aatatagtgt caattgaaac cagaagttta tgctgtttca ttaaggtttt
     2341 ctttaagctt catgaaaaaa tgagcagtaa gacaagtcct atgttttttg ttatacttgg
     2401 gaagagaaag ctaaagcagg tgaaggaatg aaagtaaaag taaaaaaaag agcaagcaat
     2461 cgattgaaaa aaggatatcc actgatccaa aaagaagacc ttgctcatcc ccaaaaagaa
     2521 accacgcaat gggtggaatt agttgattcg caaaaccaat ttatcgcaaa aggatatcta
     2581 ggaaagcaga ataagggaat cggttgggta ttgagccagc agaacatccc atttgatcaa
     2641 tctttttttg aagaattttt tgtgaagtca aaagaaaaaa gaactttctt tttttcagat
     2701 gattctacta ccgcttttcg tatattcaat ggagaaggag acgggatcgg cggtctgatt
     2761 gttgattatt atgcaggtta tgctgttttt tcttggtata acgaaacgtt atatgcacat
     2821 cgtgaattat tgattcaagc ttttcgtgaa atctacccgg aaatcgtagg aggctatgag
     2881 aaaattcgat ttgaatcagt aaatctgcct gagagccagt tcatttacgg aaaagaggca
     2941 ccggaaccct tgatcgtaaa agaaaacgga gtaaaatttg ccgtttattt aaatgaagga
     3001 ttaatgactg ggatcttttt agaccaaaaa gaagtacggg gatcattagc agaaggtttc
     3061 gcttctggga aaacagtttt gaacatgttt agttataccg gcgccttttc tgttgctgcc
     3121 gcaatgggag gggcaatcga aacaacaagt gtcgatctag caaaacgcag tttaaaaaaa
     3181 acacaggaac agtttgaagt gaatggtttg gatgtcgatc agcaaaaaat cattgttatg
     3241 gacgtattcg agtatttcaa atacgccaaa agaaaacaat ttttatatga catgattgta
     3301 ttggatccac caagttttgc cagaaataag aaaaaagtct ttcgcgtagc aaaagattat
     3361 ggggaactgg tcaaggatag tctggatatt ttagcagaag acgggatact tattgcgtct
     3421 acaaatgctg cgaatgtctc gatagaacaa ttccaaacaa tgattgaaga ggaattccat
     3481 gccaaaaatg tttcttttga acaacttgct ttatatcgtc tgccaaaaga tttccagacg
     3541 acggatacct ttttagaagg caattatctg aaagtgtttg tttatcaaat taaaaataac
     3601 cattagaagg agcgatgcag aatggtttgg gtaaagggat tgaatatagg aagcggtaga
     3661 ccaaagattt gtgcgcctct tatcggggcc gtccgagagg aagtattacg tgaagcagtt
     3721 cttgcacgtg aagcaggggc cgatttagtc gaatggcgag tcgatcatta tcgtgaagtt
     3781 ttttcttttg atgaagtagc agaaacattg gttcttatcc atgagatgct tgatggactg
     3841 ccgttattat ttacgtttcg taccttagct gaaggcggag aacaagacat atcgatccgt
     3901 cagtatcgcg acctttatga acatgtgata cacacaggat tagtagatat ggtcgatatc
     3961 gagttgttca aagtagaaag tctaggaaaa agactgatag aggaaatcaa gtctttaaac
     4021 attcctttaa ttatcagtag tcatgatttt aaagaaacac cagcagaccc tgtcttgctt
     4081 taccgattga atgttatgga acattttgga gccgatatcg gaaaactggc agtaactccc
     4141 aataatgaac gagatgtttt gcggttgatg gagttgacta gacgggcaaa tgcttttgtc
     4201 tccatgccta ttattacgat gtccatggga aatcttggta aaatttctcg tctggctggc
     4261 ggaacgacgg gttccgttat gacatttggt tccttaagca gcgattctac ttctgctcca
     4321 ggacaattac cggtcaaagc attgaagcaa attattcaat tattaaatga accgttgtag
     4381 aacaagtttg aggtgatatt ttttggcaaa gaaaaaaata gtaaaaacga atgcaatccg
     4441 tatggtcgaa caaaaaaaga ttccctatac agagcacgaa tatgagtggg acgaaagcca
     4501 cctaagtgcg tcatcggtcg ctgaacagct tccagaaagc caatctcata tatttaaaac
     4561 actggttgct gtgggcaatg tgacggggcc tcttgttgct gtgataccgg gagaagccga
     4621 attgaatcta aaaaaattag caaaagtttc agggaataaa aaagtagaaa tgctccattt
     4681 aaaagatttg gaagcaacga ccggatacat acgtggtggc tgctcgccta taggtatgaa
     4741 aaaactattt cctacttatt tagatcaaat tgcagaaagc tatgaacaaa tcattgtctc
     4801 ggctggcaga agagggctac agatggagtt agcacctcaa gatatctgtg cattaacgag
     4861 cggacaattt gcagatataa aacaataaat catacgtata aaagaaagaa ggaggccggg
     4921 atgttgtttg tccagtctcc ttctttcttt tttatacatt atgctctttg cccggcattc
     4981 attcctgcta catgccctgt agcgaaagcc gctgtgatat taaagccgcc agtataacca
     5041 ttgacatcca gtaattctcc cgcaaaatac agaccatcaa tgattttact ctccaatgtt
     5101 ttaggataaa tttcctttag agaaacccct ccgccagtta caaatgattt ctcaatagga
     5161 aaagttttgt tgattgatag cttaaactct ttgcatagtt gaacaaaatc ttgaatttgt
     5221 ttttgagagg cttgttgtcc tgtcagatga tccatttcaa gccgctcaag aaagaaaact
     5281 aacaatcgct caggcaatat accatgccaa gcattgacca agttcttttt ggttgcttta
     5341 cttttttctg tcaatatatg aataagctct tgtttggttt gtgtagggaa acaatccaga
     5401 gaaagtgtca ctggttctcc cgttttctct aattctttat tgacaaaact tgaacaacgc
     5461 agtgcagccg gaccagaaat accgaaatgg gtgaaaagta aatccatcgt atgttcagtc
     5521 aatacacgac cttttttatt taaaacgcgt aaagtgatgt cttgtaaaga aagcccttga
     5581 agtgttttct cctgaatata cggttcatcc gatatcaagg gggactctgt cgcataaaga
     5641 ggcgtcaccg tgtgacccac tcgcttcacg atcttgtacc catctccagt agaaccagta
     5701 gatggataag tccgtccacc agttgttaag ataatggcag gagcatggaa ctcttcgaat
     5761 tccgtgcgta taccgtaaat tttatgttct ttgtggatca atttctctac tcgagtagag
     5821 aatagaagcg tcacatctag ttctttcaga cgattgagca atgcttcgat aatcgttttt
     5881 gatttatttg ttacaggaaa catccgtcca tgatcctctt cttttaaatg tacgccttgt
     5941 gattcaaaga agttcatgat atcgaaatta ttccattgcg caaacgtact atataaaaac
     6001 tttccatttc caggaatatg tgcaatcaaa tcatcaacag gacgattgtt cgtaacattg
     6061 caccgaccac cacctgtcat cagtagtttt tttcctgctt ttttgttttt ttcgatcaag
     6121 agtacgcgtg cgccttgttc agctgcactg atcgctgcca tcatcccgct tgttccagcg
     6181 ccgactacga tcacatcata cagtttttcc attccctcac ctcaaggaaa gtgtagcata
     6241 tctgaagaaa gaaaacagaa aaaatgtttt gagccaaaag agaaaatgag ctaaaatgag
     6301 ggaagataag caaaggagcg tggaacgatg acaaacgcaa cagaatatgt tcaagcaatt
     6361 caagaaaagc tacatcaaaa ggatcaaggg caagaagaat ttttacaagc aatcgatgaa
     6421 ttcatgccca cggttatgtc ttttttggac actcatcccg aatatatcga aaagaatatc
     6481 ttagaacttt taacggaacc agaaagagtt attcaatttc gagtgccttg gcaagatgat
     6541 gaaggaaact ggcgagtaaa tagagggtac cgtatccaat acaactcagc tatcggccct
     6601 tataaaggtg gattgcgctt ccatcctagt gtcaatctaa gtatcttgaa atttttggct
     6661 tttgaacaga ttttcaagaa tagtttaacc ggtctgccaa tcggtggagg aaaaggcgga
     6721 agtgacttcg atcctaaagg aaaatctgat aatgagatca tgcgtttttg tcaaagtttc
     6781 atgctggaat tgtcaaaaca tatcggccca tcgattgatg ttcctgcggg agatatcggt
     6841 gtaggagcta gagaaatcgg ctatttgttc ggcgaatata aacgattgaa aaaatatgat
     6901 actggtgtgt tgacaggtaa acctttggat ttttggggaa gtaagatacg tacggaagct
     6961 actggttatg gattagtgta ttatgtcaaa catctattga acgaagaaaa agattcattt
     7021 aatcaaaaaa cagtttttgt ttccggaagc gggaatgtag ccatttatgc aatcgaaaaa
     7081 gtgcaagagc taggcgggaa agttgttaca tgttcagatt ctagcgggta catctatgat
     7141 ccagaaggaa tagatgtgaa actattgaaa gaagtcaaag aaatcaatag acaacgtttg
     7201 acggaatacg caaaccaacg tccttctgct atttatcatg ctggagaatc tgtttggaca
     7261 ttgaaagaaa aagcggatat cgctttgcca tgtgctactc aaaatgaaat agacgaaagc
     7321 ttggccgaaa tcttagtaga aaatgggatc aaaatcgtag cagaaggcgc taatatgcca
     7381 tgtacagtaa aagctgtttc tgttcttcat gaagcaggcg tgtggtattg tccaggaaaa
     7441 gctgctaatg ctggaggagt agcagtatct gctttagaaa tgagtcagaa cgcccagcga
     7501 ttgacttggg aaagagagca agtggatgag cagctggatc aaatcatgga aacgatttac
     7561 catacgtgtc gccaaacagc agccgagtac ggcgacgaaa aaaatctgct tcttggagca
     7621 aatgttgctg gatttgaaaa agttgccaaa gcaatggcag cagaaggttt agtttgaaaa
     7681 atgaaacttt cttggaaata ttgtcagcat tttgtttatt tgcttgaaaa ataataaaaa
     7741 aaaacgtaaa gtattacctg tagagtaaac ctataaggag gagaacaaat ggcacacatt
     7801 cattttgact attcaaaagt agcaccgttt gtcaacgaac aagagttagg gtacatgcaa
     7861 agccaagtaa cagcagccca caacgaatta cgggaaggta ctggcgcagg aagcgacttc
     7921 cgcggctgga tcgatttacc aacgaactat gacaaagaag aatttgcacg tatcaaagaa
     7981 gcagctaaaa aaatccaatc tgattctgaa gtattagttg ttatcggtat cggtggttct
     8041 tatttaggcg ctcgtgcagc gatcgacttc ttgaatcaca cattcttcaa tttattagat
     8101 agcaacacaa gaaaagcacc acaaatcttc tttgccggaa attcaatcag ctcaacatat
     8161 attgctgact tgatcgaagt aatcggtgat cgcgactttt cagtaaacgt tatttcaaaa
     8221 tctggaacaa ctactgaacc agcgattgcg ttccgtgtat tcaaagagct tcttgttaag
     8281 aaatatggac aagaagaagc aaacaaacgt atctacgcta caacagataa agcaaaaggt
     8341 gcagttaaag tagaagcaga tgcagaaggt tgggaaactt tcgtgattcc tgatgacatc
     8401 ggcggacgtt tctctgtatt aactgctgtt ggtttgttac caatcgctgt tagtggtgca
     8461 gatatcgatg cattgatgca aggtgccgct gatgcaagca aagcatactc aagcgacaaa
     8521 ctagaagaaa acgaagctta ccaatacgca gcaatgcgca acattcttta ccgtaaaggc
     8581 aaagtaactg agttgttgat caactatgaa ccaggaatgc aatacttctc agaatggtgg
     8641 aaacaattgt atggtgaatc tgaaggaaaa gaccaaaaag gcatctatcc ttcaagcgca
     8701 aacttctcaa ctgatttgca ctctttagga caattcatcc aagaaggacg tcgcaatatt
     8761 tttgaaacag ttgtgaaggt tgaaaaacct cgtaaatcaa tcacaattcc tgaacaagca
     8821 gaagatttag acggtctagg ctacttgcaa ggaaaagaag tggacttcgt aaatacaaaa
     8881 gcatttgaag gaacattgct tgctcataca gacggtgacg tgcctaactt gctagtcaaa
     8941 attccagaaa tggatgctta tacattgggt tatacaatgt acttctttga aattgctgta
     9001 ggtatttcag gttacttgaa tggaatcaac ccattcgacc aaccaggtgt agaagcttac
     9061 aagaaaaaca tgttcgcact tctaggcaaa ccgggctttg aagatcttgc taaagaattg
     9121 aacgaacgcc tgtaagataa gggtttttcg ctagatttaa ataatcagtg atagaaacat
     9181 gttcgcttta aaatgaaaac tggtacccat ttaggtactc aaacatactg catcgtttat
     9241 tcagaataag gggctgtgac aagacgtttg tcacagtccc ttgtttgtat agaaatcgat
     9301 tgattgcctt gctctttagt ttgttttagg actttaagaa cagtgaggct gacttcgaag
     9361 gttaattctg tttttttgtt ttctagaatc agtaagcaaa aaagcactca cttcgagtgc
     9421 ttttttgcga tatattctta gcttaagata ggcctaattt ttcttttaca gatgagggta
     9481 ccttatcgct ggtaattact tttttctcta tgatccgctt ttcgcttgct ttcaactgca
     9541 agatcgttcc ttcggtgtaa gcagaaccat tggcagagtc accagaagta atgacatcga
     9601 aatctaactg tcttttttca ccgttttcag cataagcatc tacgttgtat ttaaaacctc
     9661 ttccaattac ttccccatta tctgctttga tatcagtttc tgcaggcatt ggagctttga
     9721 ctaccgcata ataatcttta cctacataag tagactgata gtagtcccaa cctttccatc
     9781 ctataaaagc aatcaatatg ataacgatca accctaaaat ttttttcatg aaagatctcc
     9841 ttttttattt ttattatatt aaatattata cagataataa cggaatatta tcatcgaaac
     9901 agctaacaaa attgtcataa aaaacgagat ggttgatatt agaacttgtg tttttgttta
     9961 ggtctagaga taaagagaga caatttccaa atatatgtaa tgaatacaaa aagacgtttg
    10021 tcgcggatta ccgttacaaa cgtcttttat tgattttccg agatctcatc agtcgccatt
    10081 tggaatggtt cactccttat ctagattcgt ttaatgctcg gactgcttgt tcgattgtaa
    10141 taccaaacgc aacgttattt tcgttttgtg catcaatcgc cataaaacgg ttagaagttg
    10201 tatcaaaagc tacgcggtat tgcatatttg tttttgtaaa cgtcataaaa ttcatctcct
    10261 ttgtaatgta atttagttct tgaaagtttc ctttaaactt tcgaacttat accaagttta
    10321 gcatcgtacg ttttttgatg caattaaaaa gcatttatca ttttccttat ttattttttc
    10381 ttaaatggtt tagctatttt cttttaatcc gataagtagc ccaatcatca aggttacaaa
    10441 tactattgct tgaatataaa tcatatgctc acctctttat aaataactta gcaatataaa
    10501 gaaaaaaacg cacgaacaac ataggaatat aaacaaacgt ttttgtcaca acgtctaaga
    10561 tcgtgttaaa aaatagttat ctaaaaagca gaaagatact caaaataaaa aataaaaaaa
    10621 tgaataaaaa cgttttcaaa tacaaggtaa tttgttatta taaaataaag aaaaaacaaa
    10681 ataaaaataa agaaaagagg gaatgaaatg gtaaaagcag aagtaggaag atcttatata
    10741 tgcaaaccga tcggagtatc tcatgaacta atcggctgta ttgaacgtac atatatcaac
    10801 acggctgtcg tgtatgttga aagttatgac caacgagact atcaacttat caatgaaagc
    10861 aagtatcgca tgctcgtaaa attctgtgat attagcgcac ctactgaatt ggtttcttaa
    10921 acagtatttt tgagaattcg caagaactcc cttataatat aaataaagac agaagggagc
    10981 gagaagatat gccaaaaaga aaaagttcag ggaaaatggg aattattctt gcagtgatct
    11041 ctgcaattcc tgctatttta gctattgttc gtgcgatgaa ggaatcaaga gaaagttaag
    11101 cacacattat gagcgtcaaa caaaaaacgc atatacctgc ggtggcaggt tatgcgtttt
    11161 ttgttgtata aagacttttt tcattaacga tttttgatta gccgactccc aaaacaaaat
    11221 ttagcagttt atctgaataa gtgatttctt catctgtttg tttcacactt tcatttagta
    11281 gtcccatctc atcggttatg ataccatcca caccataaaa catcatgcga ctcatggcat
    11341 ctgaatcgtt gattgtccat acatatactt cttttccgtc ttggtgggca gaattaatga
    11401 agttacggtt gatcgtacta tattccattg tcagaaaatc agcaggcgtc acagggggcc
    11461 caacaatatt gaaaggtaag atgtagccca cataaaagtt tggttctgct tcttttaaat
    11521 ctgaaacggt ttggtacgtc aaactttgca aaatatgatg gttctctaag ataactttgc
    11581 ggtatttatg gacaaatcgt tcgacaaggt catccgaatc ttttttagag gtcttgatct
    11641 caataagtaa tttttggttt agcttatgtg ctttttccaa ataagaatca aaagaacaaa
    11701 caggggcttg cactccgttt tcagtaactg taagatttgt caattctttc aatgtaagat
    11761 cctgtggtgt tccatttacc cctgttaagt gacgaagatt tgcatcatgc atcactacga
    11821 aatgaccatc ttttgtttcc tgaacatcca tctctacata atcaggatga tagtctctgc
    11881 ttgttttttc aagtgcttct aaagtatttt gcgccccatt ttgggtactc acacctctgt
    11941 gagagacagt cactggtttt gtctgaacag ctgaagtcag ataatccata ttgtaaagac
    12001 agacgccaat accgaaaaaa actgcaaaaa gagtcaaacc agtgtttttc agcgcagaga
    12061 aacgcaagtt gggggcttgt gggataaacc acttagggat ctcaggcaag aagccttcgt
    12121 catccatgaa atcaacaatg atataaaaaa tccctacggt agacatcacg atattgaata
    12181 ataaaatccc ttgtaaaagc gtcatagcaa acacagcgct gatcaatgca tagtctggga
    12241 aaaattgttc aaccatactt tgtgccaaaa taacaacgat atatcctaaa gaagaaagga
    12301 gtaaaatcgt accgccaatc acaataaatt ggcccgtgat ggccaaaaga cgcgatttgg
    12361 tcaatgacca gctttcccga atggctgctc gaaaagaacg atctcgtaga atcatttccg
    12421 gtaaagcaaa aatcagtcga atcccaatat agccaaggaa aatataaacg agtaaaaagg
    12481 aactgacgat gatccaacga ttagcaaaaa taaaatccat aatgaaagcg gggattttta
    12541 tttttgacag tagatcagaa ttaaaactta atccgctgat cggtagaatc aataaaaaat
    12601 aagcaaggaa aaacaaaaaa gtaatgggtc tcactttctt cagctgaagt atcgtcaagt
    12661 gtaagagttg cttcaaagag attggttctt tctttttaat aaaaaaaaca ctcatcaaaa
    12721 gaaaagtaaa ttcgaaataa actaacagta aaatcgtcat caaaactacg attaacgaga
    12781 gtagaacccc aggatgttga gaaaaaattg aaggaatatt gtcataagac aaatagtcaa
    12841 ttgcccctcg gtgtaaaata aaacgggtcg tactgctcaa taagggtaaa caaataaata
    12901 aaataaagcc atgcatcagc aagacatcac ggaaataagc ttgtgttcct tttaaaaagt
    12961 cccaagcatt acctaagcta cgtttcaaat aattcatcct atcacctgct cttcacattg
    13021 tagcgcaaat ctaatgaaaa aggaaagggt gcagtgtgtt ttgcagaccc tttcccagat
    13081 ttaac
//
LOCUS       DAYYGO010000079        12738 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000079 DAYYGO010000000
VERSION     DAYYGO010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.081"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACHA5T_001953"
     CDS             complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACHA5T_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFM8887518.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(323..892)
                     /locus_tag="ACHA5T_001954"
     CDS             complement(323..892)
                     /locus_tag="ACHA5T_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFM8887519.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1078..1980
                     /locus_tag="ACHA5T_001955"
     CDS             1078..1980
                     /locus_tag="ACHA5T_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFM8887520.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="ACHA5T_001956"
     CDS             complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="ACHA5T_001956"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFM8887521.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="ACHA5T_001957"
     CDS             complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="ACHA5T_001957"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFM8887522.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5003..8317)
                     /gene="dnaE"
                     /locus_tag="ACHA5T_001958"
     CDS             complement(5003..8317)
                     /gene="dnaE"
                     /locus_tag="ACHA5T_001958"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFM8887523.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8548..8733
                     /locus_tag="ACHA5T_001959"
     CDS             8548..8733
                     /locus_tag="ACHA5T_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFM8887524.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8795..9991)
                     /locus_tag="ACHA5T_001960"
     CDS             complement(8795..9991)
                     /locus_tag="ACHA5T_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFM8887525.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10161..11597)
                     /locus_tag="ACHA5T_001961"
     CDS             complement(10161..11597)
                     /locus_tag="ACHA5T_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFM8887526.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11890..12699
                     /locus_tag="ACHA5T_001962"
     CDS             11890..12699
                     /locus_tag="ACHA5T_001962"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8887527.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2673 c         2250 g         4012 t
ORIGIN      
        1 tattttttat ttaccgacta caaaaaaata aacctcaagg ctccatggtt agccttgagg
       61 ttttacattt tctaattaaa atgtattaag cttcttttga cattacgtcc ttaccatcat
      121 agtaaccaca atttgcacat acgtgatggc tttttttcat ttcaccacag tttggacatt
      181 cgtttaaccc tttgattgtc aatttgtagt gtgtacgacg ttttgctttt ttagcttttg
      241 atgttcttct agctggtact gccattttgc tacacctcct tgtaaagtct tgtatctaaa
      301 ttagacacaa tatattccaa tcttacttgt tatcgtcgtc ttttgattta gaatcaaaaa
      361 gttcagatag ttttgcaaga cgaggatcaa ttttattctc ggcttcttcc atttttctgc
      421 gctggtattc ttcttcggaa acaacttccc aatcattgcc ttttggaagt tctgttgctg
      481 cttcctcttc ttttgtcaat acccgcattg gaatcgccaa taagatgttg tcttcaatcg
      541 actctgctaa attcaataat ggaccttcaa ttagcaaaat ctcatctgca gacatttgtt
      601 catctctact ttggaattgt tcttctgtca tgaatacttc gtctacagaa aaatccatgg
      661 gcagttcaac aggttctagt gaacgtgttg aaggaactgt caaagtcgtc tgcacagtgt
      721 aatgaacgat atagcctttc ttatcaactg agaccaaacc ttcgacttcc actggcgcaa
      781 catcaagtat taaattgtct cgcttcatga gagcggcttt caagtcgagc gtctcatgaa
      841 aagttaacgg tgtctcctgg tattttctca attccaataa agaccatttc atttttcatc
      901 actcctaagc aacaaaagca attatacagg gcaaaaagca tgttgtcaat gaaattttct
      961 tgacaccttg cagttcataa aaaaacagaa aaatctttat tagaaagcct ctgtttgcta
     1021 tcatcctcag aatcatttac aataatcatt attccattaa aggagcggat gatcttcatg
     1081 aacacgaaag atatcgaata ctatgtccaa atcgtcaaac aaaaaaattt tacgaaagtg
     1141 gctaattatt tccgtgtgag ccaacctact attacatatg cattacaacg tcttgaaaaa
     1201 gagtttgaca ccgtacttat tacaagagat cgatcgcatc acgaattaat cgtcacgcct
     1261 tctggaaagc agctgtttct ccatgcacaa accattctcc aagagatttc tactgcaaga
     1321 gctgaaatca ctcagttgca aaagaaaaag cttcgttttg gcatgcctcc tattatcggc
     1381 aatgaatact ttcctaaact gtcaagttac tttatcaagc atgaattgat gaatcaaatt
     1441 gagattaccg atggaggctc tcgggactta tacgggatga tccgacaagg gaggatcgat
     1501 ttagctgtat taggctcaac acagccaatc gtggatcaat atttagaaac agagttactt
     1561 ttggacaaac gtttcatgat cgtcgtgcca cctacacatc ctctttccaa gcaggatact
     1621 gtctctttca aagatatagc agatgaacag tttgtactat tgaacgaaca ttatgtccac
     1681 cccgcagcat tcactaagct ttctaaacaa acacatactc atccaaaagt tgtttatcaa
     1741 agcaatgact tgacgatttt aaagagtatg gtccgagaaa atgtgggtat cggttttttg
     1801 gcagagattg ctgtccatcc tgaagatcat ttagtttgcc tccctctttc agatgaagtt
     1861 cagcctcgct tcttgatttc attggcaaaa cgaacacagc agatcgtcac gcctcttcaa
     1921 gaacaagtag tccaagttat ccatgaattc gttacagcac aaaaaaacag ctcttcgtaa
     1981 aagaagagct gtttttattt tttattaagc taaattagat agctgttgtt tcgccacggt
     2041 aaacgatgcc gcgacgtgaa tcaacggtaa tcaattcacc ttctgtaatt gttgttgttg
     2101 cgcctttagc accaacgatt acaggaatat cttgtgcaat tcctacaact gcagcgtgag
     2161 aagtcaatcc gccttcttca acgataacag cagctgcttt ttcgattgca ggcatatatt
     2221 ctttgtcagt tgatggtaca actagaacca ttccgtcttt tgcttttttt acagcttctt
     2281 cagcagatga agcaataact gcatttgcaa cgacagattt ttgtccaacg ccttgagctt
     2341 ctaataattt agaaccaatc atttgaattt tcatcacgtt agttgttccg cgttcaccaa
     2401 ctggtacacc agcagtgatc aagattaaat cgccttctga tgcgaaacct aattcaacag
     2461 cttttttagc tgctagttca aacatttcat cagttgttga tggtttttca gcaactgttg
     2521 ggtaaacgcc ccagtttaac atcaaaccac gttttgtgcg ttcgtcaaaa gtaacagcca
     2581 agatatctgc atctggacga tattttgaaa tcatttttgc agtgtatcct gattcagttg
     2641 cagcaacgat tgttttcacg cctagatttt ttgcagcacg agctacagat aaaccgattg
     2701 tttcagttac atctgtttta tcgaattcgt tcaattggaa tgtacctaat tctgataatg
     2761 cattttcagc ttcttggtcg atacgtgcca ttgttgaaac agcttcaact gggtagtccc
     2821 cgtttgcaga ttcaccagat agcattgttg catctgtgcc atcaaataca gcgttagcaa
     2881 cgtcagaagc ttccgcacgt gttggacgtg ggttttcttg cattgattct aacatttgtg
     2941 tagcagtgat aactgggata ccagcagcat tacatttttt gataatgcgt ttttgaacca
     3001 ttggtactag ttcagctggg atttcaacac ccatatctcc gcgggcaatc ataacaccgt
     3061 cagaaacttt gatgatttcg tcaatgttgt cgataccttc ttgagattcg attttaggga
     3121 agatttgaac atgagtcatg tctttttctt ctaagatttc gcggatatct agaacgtctt
     3181 gtgctttacg tacaaaactt gcagcgataa agtcgatatc gttgtctaaa ccaaaacgaa
     3241 tatcgtcagc atcttttgga gtaatacctg gcaaactaat agaaacgcct ggtgcattta
     3301 cccctttacg tgatcctagc atacctgcat ttttaacttc tacgactaat tcacggtttg
     3361 cttcgtcttt ttcaatgatt tgcatatcga tcaaaccatc atcaaataaa atgtgtccgc
     3421 caacgtgaac atcgtcaaat aatcctgggt aagtaaccgc gattttttct tttgaaccta
     3481 catgttcagg atccatagag atgcgtgttt gatctcctac ttcaaatttg atgtatcctg
     3541 cacgaccaaa atcagcttct gttgtgcctt gaacagttgt acggatttca gctccttttg
     3601 tatctaaaag aataccgaca tcttttcctg tctttttaac tgcttcacgg accatagtca
     3661 tacgtgataa ttgttcttca tggtcaccat gagaaaaatt aaagcggaag acatttgccc
     3721 cagattcaat caattgagaa ataatttcta ctgagttact tgctggtcct aatgtactaa
     3781 cgattttggt ttttttcatt taaaaaactc tcccattcaa ttttgtttat ttaaagctgt
     3841 acacagctta aatagcttgg tttctttcac tcttaaaatg aaagtgaatt atttagatca
     3901 tatagtgata agtctggttt atgtttgttg ttttcaagtg tatcaatgat gtcagacgca
     3961 acgatttcgt tgtcatggat accgacacat aatccacctt ttccttcttg aagcaattcg
     4021 acagcgtaag caccgaattt acttgccaac acgcggtcgc gtgcgcttgg tgctccacca
     4081 cggacaacgt gtcccaagat agatacacgt gtatgatagt caccaaattc tgaaagtttc
     4141 tcagcgaatt catttccgcc cattacacct tcagccaaga tgatcaagca atgtttcttc
     4201 ccgcgatcgc ggccttcttg gattttttta gcaactgaag ccatatcaaa atcatgttct
     4261 gggatgatga tttcatccgc tccgccagcc acacctgacc ataaagcgat gtctcctgca
     4321 ttgcgtccca ttacttcgat aacgaatgta cgaacatgag aagtcgctgt gtcacgaata
     4381 cggtcaattg attctagtac tgtattgatt gctgtatcaa aaccgattgt gaaatcagtc
     4441 ccaggaatat cattatcgat tgttccagga atacctactg ctgggaaacc gcgttttgtc
     4501 aaagccattg cgccatgata agagccgtct cctccgataa ccactagacc ttcgattccg
     4561 aattttttca attgttcgat tccttttaat tgaccttctt cagttgcaaa ttctggataa
     4621 cgtgcagaat acaagaatgt accaccacgt tggatcttgt cacctacatc tgccacatca
     4681 agacgacgaa tatctccagc aactaaaccg gcaaaaccat aattgatccc gtatacttcc
     4741 agtccttcgt aaataccttt acgaacaacg gcacgaacag ctgcattcat tccaggtgcg
     4801 tctccgccac tggtcaaaat tccgatgcgt ttcataataa acattcacct cattaaaata
     4861 ctttatccta agtatgactt aggtttacaa ccatttttgc gttaaccacg actaacattc
     4921 taacattatt tttctcactt gtcttgattt acggataaaa aaatgaaaaa aaatgaaagc
     4981 aagtagtatt ttttaccgta agttatttga aaaccacatt atcttcgcca agcagataaa
     5041 taagttgccc ctttatactc ttgctgtcat ccacccaata agcatctgac agaactactt
     5101 ttcgttgcgt tttttcgtga tacataacta ctggtatata tcctgggtgt ttctgaaaaa
     5161 tcacttgtaa ttgtttcatt atctcgggat cttcccgttc ttctgtaata cgcaaatagc
     5221 aagtttgtga aggatactgg gcttctaact ggttaggatc agcaaagctt tctccaatta
     5281 gttgcagttc ctgattatat ttgctgattt cgctccgccc ttgaacaaag atcactcgat
     5341 caacggcaaa caaattacga tatttacgat aaagagttgg aaaaactgtg acagaaattt
     5401 ctccactact gtcggttcct tcaacaaagg ccatcagttc tccttttttt gtccgaattt
     5461 ctctaacttc cttgatcatc actaataaat tgactggttg attagggacg atatctgtaa
     5521 tcagctgaac ttgtttagct aaacgaagac gatcaaaccc ttctgcagga tgacctgaca
     5581 aataaatacc taagtactcc tcttccagct tcaagcgttc ttctagtgta tagtcagcga
     5641 cttcttcttc ttttaatgcc atgattccca gcaagtcaag acttccacca ctgtaaacga
     5701 tattttggat ttttccttcg agatcaagca tcaattgttt cctgttagga gataattcat
     5761 caaaaactcc tactgctgtc agtgattgca gtaactctaa tttcagccaa cgcttatcga
     5821 tccgtatgag gaattgatcc actgactgga acgggccatt ttgttttctt tcgtttaata
     5881 tatcttctac aaaatcacgc cgaatacctt ttatcgctgt cagtccgaat ctgatttctt
     5941 ccttattgac caactcaaaa ctggaattac tttgattaat ggacggcgag gaaagtttga
     6001 gtttattttt acgtgcttct gcaatatatt ctttcaattt tgatgtgtta tgccgaacag
     6061 aattcatcag gctggtaaag aaagcacctg gatgatgggc ttttagatac gccatttgga
     6121 atcccacgaa tgaataagca aatgcatgtg accgattgaa tccatagttt gcaaattttt
     6181 cgatatattc atatacggtc gctgctgttt tttccgaata tccctgttgt aatgctcctg
     6241 atacaaaatg cgacttttct tcatctaata cgtctttttt ctttttactc actgcccgtc
     6301 gcagaatatc tgactgccct aatgtaaatc cagccatagt cgaggcgatc tgcatgattt
     6361 gttcctgata gacgatcact ccatatgtgt ttttcaaaat cgggatcagt gaatcatctg
     6421 ggtagcgaat ggcttctttt cctttcttcc tggcaatgaa ggtatctata ttttgcatag
     6481 ggcctggtcg atacaatgca ttgactgcgg caatatcttc gatgttttct ggatctaatc
     6541 ttcgtaaaac gttccggata ccagcagatt caaattgaaa tactcctgag gtttcgcctc
     6601 gttgaaacaa gagcagcgtt tttgcatcat ttagcgggat ctcttttaat cggatctcat
     6661 acccttctac tcgcttaaca gctttcaaag taaaatcaat aatcgataag ttgcgtagtc
     6721 ctaaaaagtc cattttcaaa agaccgattg cttctacctc attcatcgta tactgggtca
     6781 gaaaagcttc atcagaaccg cgttggagcg gaactagttc aagtagattc ttatcgctga
     6841 taactacacc tgctgcgtga gtcgaaacat gtcttggcaa tccttctaaa atcactgcag
     6901 ttttgaataa aagttggttt ttttcgcttg cctgaactaa ttctactaat tgatgcgaag
     6961 ttttataagc ttgggtcaat gtgattttca attgattggg tattgctttt gaccagcggt
     7021 ttgcttcact ttgcgataaa ccaaacactc tagctgcatc tctaagaacc atttttgctg
     7081 ccattgtgcc aaatgttgcg atttgtgcca tatgatattg tccatacttt tctcgaacat
     7141 aattcaatac ttcttcacgt cgattgtcag gaatatccaa atcgatatct ggcatcgtaa
     7201 agcgttcggg gtttaaaaag cgttcgaaca gcaaatcata ttctaatgga tccacatctg
     7261 tgatttctaa cacataggct actaatgaac ccgcagcaga accacgacct gcaccggtaa
     7321 cgattttgtt tctatgagca aaagccatta catcccatac gatcaagaaa taatcatcga
     7381 atcccatttg atggatgaca gataactcgt agtccaaacg ttcttggtat tcctttgtat
     7441 agtcagtgat tcggcgttcc acttgcgaat agcataactc tcttaaatat tctccagctt
     7501 gttttccatt aggaactgga taatgcggaa gtaatttctg atggaactgg aattcaaatg
     7561 aaatcgattc agctaaacgc tgggcgccta ttacagcttg ggaaagaccc gctttttcga
     7621 atcgtttgat catttcttgt tctgaaagaa gatagacatt tgctgttagt ggtaaagatg
     7681 cagataaatt ttctaaaggt atttggcttc cctctttgat atgtttcatc acttcaagtg
     7741 caaaaagttc ttcttgttgt aaactagcga tgggatgtaa tgcacaaagc gaataatttt
     7801 cttttatcac atactgatac cattcaggca attgatcagg cgtgttttct gtcaagccga
     7861 tataaaaatt tctagtaaaa acattttcaa gtaaaaactg ccaatgaccg aaaaagttat
     7921 cctctttatt gaaagcctca gaaacagaat gatttgccgg aacaacaaac gctagatggt
     7981 cggtttctgt catttctgca agttccaatt tttcttggat catttttcgg ctggaaagtt
     8041 tcatcagctg ctgataacct atttcatctt ttgcgtacgc aagcaagcca taattttttt
     8101 cttcataagt ataactaaga tgtaatccaa tgattggctg tactccttct tctacacaaa
     8161 tacgatagaa ttcaggtgca ccgtataaga catctttatc cgttatggct aatgaagtgt
     8221 agccttgttt ttttgcttct tgtacatatt ctcgaatacg tatcgtactt tgtaaaagag
     8281 aataagaagt aattgtataa agttgtggga aaaacatctt gcattccgcc ttttttttcg
     8341 ttaaaattca ctagcgttaa aaggtttgat ccttttactt tcattaagat agcaggcgct
     8401 gaagttgatt attccacttc atttgcttgc ttgtaatctg taggaatgag ttctttcgca
     8461 agaactcaaa ttttattgag atttggtctc gtttctttac attcaaagaa aatctggtaa
     8521 aattaagata gaaaagaggt gttctttatg cgatatatcg ttacattatt ctgggctgtc
     8581 gttctaggtc aagtagtcgg ttatattggt gctgcattaa caagcggaac atatgatttt
     8641 actttgacaa caatcatttc attcattgcg ggtgtcatta tccttttgat tggtgcagtt
     8701 gcgccaagaa aagaaacctc tgcccattcg taaaaaaaca gccaagagct tgagatttct
     8761 caagctcttt tttattttct actcctcacc accattataa tctgctgcga agaactttac
     8821 gaaagttgaa taaagaaaga agaatatttg acagaacaaa caaacttgtc acaataaaaa
     8881 cagcactgta attataagca tgcgcgactg tcgatcctag catgggacct aaaacttgtc
     8941 caaagttgct aaacatctga ttgtagctat agattcggct gactccttct tgaggcgtaa
     9001 tcttactgat cagtgtattg acagaaggca tcaaagcgcc agttgaaaag cctaaaataa
     9061 agcgaagtat acctaattgg agcggtgtag tgacgaatgc cataggtaaa taacagcaaa
     9121 atgataaaat gagtcctcca attagaactt tttgattccc aatcttatcc cctagttttc
     9181 ctagtttcgg tgaagaaaag acagcggaaa caccagctat cgaaacaatc aagccgctga
     9241 caaaaaggat attttctgta cttccgctca attcacgaat atataaggtc aaaatagggc
     9301 tgatcgtagt gattcctatc tgtaaaatca aggtcgtaat gaagagaccg atcaaaatag
     9361 agagatgatc catttttgaa aaaatttctt tggtgcttat cagatctttt ttctcgattg
     9421 gatggaaatc ttctttgact aagaaaatgg tcaataccgt tgtgatcatc aacaaagccc
     9481 ctgtgatcaa gaatacattt tccataccaa accactgagc cagtgcaccg cccattgaag
     9541 gtccgatcag atttccagca atcgctccgg tagacaatgt tcctaacgcc catccgctct
     9601 tttctctagg tgcctgtgaa gcaatcatcg ctgtggcatt tggaatatat ccagacaaaa
     9661 tcccattcca aaaccgcatg atcaaaagcc aatacgcgtt tggtacaaaa gccaaagatc
     9721 ccattgttaa tgtcatccct gctgctgcac ggatcatcat gagttttcgg ccttttcgat
     9781 cagctagatt tccccagatt ggtgcaacca cggcagatgc aaaagcagtc acagagattg
     9841 ctagtcccga aaacaattcg atttgatctt ttggtgttcc tagttgctca acataaactg
     9901 gtatgaatgg cataactaaa ctaatacttg caccagtgaa aaaacagcca atccacgcaa
     9961 tcactaaatt ttttctccaa ttaatctcca tctatcatcc ctctttcgtc tcttttatca
    10021 tacctatttt cgaataaaca agcaacgatc tcgccagtaa aaagagaaaa atcccgcaaa
    10081 ggtcatttta tatgtaacgt tctttttatg taaaacaaaa agagatcaga acaaatactg
    10141 ttccaatccc ttctctttag ttaaattgtt gctagaatga tgaagttgat aatgaataaa
    10201 atatcaacga tccacaaagc aacagaaact tgattgtatt ttcctttgat tactttcaca
    10261 attgtataga aaataaatcc tgctgcaatc ccataggaaa tgctatagca caatcccata
    10321 aagatcgatg caaagaaggc cggtaaagct tcttccatat ctagccattt gatgtctgca
    10381 aaagaagcca tcatcattac tccaactaaa atcaatgctg gcgcagttgc ttgatttggt
    10441 acaatggcga tcagtggaga gaataaacta cttaatgcga atagcactgc cactactact
    10501 gaagtcaaac cagtacgtcc accggccccg atcccagctg cagattcgac ataagttgtt
    10561 gtattcgatg taccaaagat agcccccaca gaagtagcga tcgcatcggc aaatagtgct
    10621 ttatccattt ttgttttgaa cccacgacca tcttccaacg cgatctcgtc ttcttttgag
    10681 aaaatacctg tgcggcgtcc tgtaccgata aatgttccga tcgtatcgaa agtatcagat
    10741 aaactgaatg cgatgatcgt catcaatact tgaggaatct tggaagaatc actgaataaa
    10801 gattgcatgc cttcagaacc aaatgctgca ccgaaagttg ttcctaattc tttaaatgaa
    10861 ttacctaacg aattcgtttg ccaatcaatg gaacttaaat caacaacacc cataaaaata
    10921 ccaaggactg tggtcgctac gataccgata agaatagccc cacggacatt ttttacaact
    10981 aaaatggctg tcagtaccaa accaatcaca gccaataaga taggagcgtt gtcaaagttg
    11041 gctaacgcag ggacgattcc accatttatc gtaacatttg tcgcttttcc gccttcaacg
    11101 acagagctcg aaatagctga ctggtctgct gaaaaagata agaatcctgc atttttgatt
    11161 ccgacatacg caacgaaaat accgatcccg ccaccaatcg catgttgaag actttcggga
    11221 atcgcccgaa taatcatttt tcgaatattt gtgacagtaa taaaaatatt gattaatccg
    11281 caaataaaca ccattgccaa ggcttgttgc caagaataac caagcccaaa aacgaccgta
    11341 aaagtgaaaa atgcgttcaa tcccatacca ggtgcctgag catatggtac atttgcgaac
    11401 aatcccatta ccagcgtacc aataatcgat gcaatgattg tcgctagaaa tactgcttgg
    11461 aagggcatac ctgatgctga caaaatcgtc ggatttacga ataagatata actcatggca
    11521 aagaatgtcg ttactcccgc cattatttca gttgatacat ttgtattgtt tttctttaaa
    11581 ccgaaaaact tttccataaa aagttccact ccctgaattt ttttctgatg gaaaactata
    11641 ataataaaaa ataagtaaaa tagggcaaat ccatcgaaca atttcaaatt atatagacgt
    11701 ttcattcgct tttcaacgtt tttttaacaa aaaaagcgaa aataatactt aacgttcgtg
    11761 tttagcttat gtttagctat ttatatagcc ataactgtag tttttgttag gaatagctga
    11821 ttgctcttgt taattaccta aaatatgcta gactaaaaca aatgctagtt gaggaaggaa
    11881 ggacccttat tgaataaaaa attaattgcc atcgatctgg acggaactac cctcaatcaa
    11941 gattcgatga ttacatctaa aacaaaagag actctaaaaa aagcaatcaa tgccggtcat
    12001 catgtcagta ttgctacagg acgcccattc cgaatgagcc atcagtttta ccaacaatta
    12061 gaattaacaa caccgatggt taattttaac ggcgcattag tacacatccc taatcagcac
    12121 tgggatggtg aaagagaaac gttgatcaat cgtgaaatcg cttttgaaat cttatcgcaa
    12181 aaaaagcagc taaatttaga ttttatcgct gctgaaaatc gtgatacttt ttttattgac
    12241 agttttgatt tctttgatga aaaaattttt gcttcttctc gtccaggaga gaagaatttg
    12301 ctttctccta aaaatttgac gacaaatcct acatctttgc ttgtccgaac agacaaacgt
    12361 tttgcaggag ctgtatctgc tgaattaaca cgtcaatttg gcagttatgt cgatgttcgg
    12421 acatggggtg gacctactgc catcttagag attgtttcaa aaggaatcca aaaagcaaaa
    12481 ggcgtgcaag agattgctaa ctacctgtct atcgatcaaa aagacgtcat tgcctttggt
    12541 gacgagcata atgatttgga attgttggat tatgcaggtt ggggagtagc tatggctaac
    12601 ggtactgacc aactcaaagg cattgcaaat gatgtcactc ctttgagcaa tcaggaagac
    12661 ggtttagctg tttatctgga aaaactactt aaactataat agagaattag attccacaaa
    12721 aaagaggttg tgacaatt
//
LOCUS       DAYYGO010000080        12496 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000080 DAYYGO010000000
VERSION     DAYYGO010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.082"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      198..364
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            512..1069
                     /locus_tag="ACHA5T_001963"
                     /pseudo
     CDS             512..1069
                     /locus_tag="ACHA5T_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            1127..3166
                     /locus_tag="ACHA5T_001964"
     CDS             1127..3166
                     /locus_tag="ACHA5T_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFM8887528.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3275..4426)
                     /locus_tag="ACHA5T_001965"
     CDS             complement(3275..4426)
                     /locus_tag="ACHA5T_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFM8887529.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4580..5110)
                     /locus_tag="ACHA5T_001966"
     CDS             complement(4580..5110)
                     /locus_tag="ACHA5T_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFM8887530.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(5209..5559)
                     /locus_tag="ACHA5T_001967"
     CDS             complement(5209..5559)
                     /locus_tag="ACHA5T_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFM8887531.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5716..7245)
                     /gene="ahpF"
                     /locus_tag="ACHA5T_001968"
     CDS             complement(5716..7245)
                     /gene="ahpF"
                     /locus_tag="ACHA5T_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFM8887532.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(7255..7818)
                     /gene="ahpC"
                     /locus_tag="ACHA5T_001969"
     CDS             complement(7255..7818)
                     /gene="ahpC"
                     /locus_tag="ACHA5T_001969"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFM8887533.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7962..8624)
                     /locus_tag="ACHA5T_001970"
     CDS             complement(7962..8624)
                     /locus_tag="ACHA5T_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8887534.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            8808..10454
                     /locus_tag="ACHA5T_001971"
     CDS             8808..10454
                     /locus_tag="ACHA5T_001971"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFM8887535.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            10445..11527
                     /gene="uxuA"
                     /locus_tag="ACHA5T_001972"
     CDS             10445..11527
                     /gene="uxuA"
                     /locus_tag="ACHA5T_001972"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM8887536.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNEYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(11635..>12496)
                     /locus_tag="ACHA5T_001973"
     CDS             complement(11635..>12496)
                     /locus_tag="ACHA5T_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HFM8887537.1"
                     /translation="TSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNIL
                     ARLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLA
                     FARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVIL
                     EKENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQN
                     DFMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMD
                     QSKKENQVTS"
BASE COUNT         4178 a         2304 c         2338 g         3676 t
ORIGIN      
        1 tataaaaaaa cacataaaac taaaaaatta tcaaagaatc ctggtgcaac tgaaagaatt
       61 tgcaacggga ttctttcaac ttatttgtta tttttttcta aaaaatatta aagattgtta
      121 aatttcacta tttcatttca aattccattg cctaatcatt aggaaaagtc tatcatgaac
      181 tcgtaaatag aatatagtaa taaccttgtt aggtgaggct cctatacaaa cataggctgt
      241 tgcccagaaa cgtcgaaagt cgccaatggg taaaacagaa actgttggat taaggctttt
      301 tccaatagct aagaatgttt tctttacgtt gtatagtgct aaaactcgga cgatgagatg
      361 atgctaggtc agaattcttg ttcgaaaagg tttctgacct tttctttata tttttgtgac
      421 tgacttattt gatgagacaa actcgctcct cacctgacaa gtggattgct gtgtgaggag
      481 gagatgcggt gaaaaaagat cgattggtgg aggtcgctcg gattcacttc tggttgcaca
      541 atacaaccag aaaacacaac aagcaaaact gatctctatc atgagagatt catatgtcga
      601 tattccaggt tacggaatga ataagataaa tgcagcttac tcttatgggg gagttgattt
      661 attaaatcaa acattaaagg aaaatttcaa atttgaagct ccctattatg caagtatcac
      721 atttcaagat tttatcgatt gcgttaatga actgtttcct ggtggagtca agatcgatgc
      781 agaaaaatct ctagatttag atggcgtata tataaacaaa ggagagcaag tgatggatgg
      841 taatacttta ctccagtatg ctcgatttcg ggaagacaaa gaaggagact tcgggaggat
      901 cagaaggcag cagcaagtga tcaaagctat ctctcagcag ctgaaagacg taacttcaat
      961 attcaagcta cccaaagcgg ttgggaaatt acttggtagt atccaaacga accttccaga
     1021 aagtgtgctt ctggattgtg gtatggattt cctcaaagac atatgttaga ataaacaggt
     1081 ataaatagtg aaataaagga atgacaagca agagaaggag gaaaaaatga aaagaagtga
     1141 caagcacggc aaaaatcgaa caggcgctta tattgccggc gcagtgatct taatagcagc
     1201 tgcgggtggc ggttattttt actaccagca ctatcaagaa acccaagcag tagaagctgg
     1261 agaaaagaca gttgagcaat ttgtccaagc tttgaacaag ggagattata acaaagctgc
     1321 agaaatgaca tcaaaaaagg cggcaaataa aagtgcatta tctgaaaaag agatcttaga
     1381 taaataccag aatatatacg gtgctgctga tgtcaaagga cttcagatat cgaatctaaa
     1441 agtagataaa aaagacgatt ctacttacag tttttcatat aaagcaaaga tgaatacctc
     1501 attaggtgaa ttgaaagatc tttcttataa aggaacatta gacagaaatg atggtcaaac
     1561 cacgatcaac tggcaaccca acttggtttt tccagaaatg gaaggaaatg acaaagtaag
     1621 cctgaccaca caagaagcag caagagggaa tattatagat cgaaatggtg aaccattagc
     1681 aacaacaggc aaactaaaac aattaggagt cgttccaagt aaacttggag atggaggcga
     1741 aaaaacagcc aatatcaaag cgattgcttc ctccttcgac ttaacagaag atgctatcaa
     1801 tcaggcgatt tcacaaagct gggtacaacc cgattacttt gtcccattga aaatcattga
     1861 tggagcaacg ccagaacttc cagctggagc taccattcaa gaagtagacg gcagatatta
     1921 tcctttgggt gaagcagctg ctcagctgat tggttacgtg ggagatatca cagcagaaga
     1981 tattgataag aatccagaat taagcagtaa tggaaaaatc ggtcgctcag gtttggagat
     2041 ggcttttgat aaggatcttc gtgggacaac aggtgggaaa ttaagcatta cagatgcaga
     2101 cggtgtagag aaaaaagttc tgatcgaaca tgaagttcaa aatggcaaag atatcaaatt
     2161 gacaatcgat gcgaaggcac aaaaaatagc ttttgacagt ctaggaggaa aagctgcatc
     2221 aactgttgcg acaacgccaa aaaccggtga tcttcttgcg cttgctagct ctccaagcta
     2281 tgatccaaac aaaatgacaa acgggatctc acaagaagat tacaaagctt atgaagaaaa
     2341 tcctgaacat ccattcatca gccgatttgc gacaggttat gctcctggat ctacgtttaa
     2401 aatgattaca gcagcaatcg gtctcgacaa cggcactatc gatccaaatg aagtgttgac
     2461 gatcaacggg cttaaatggc aaaaagacag ttcttgggga tcttttaaaa caacgcgtgt
     2521 cagtagtgat gtatcacaag tagacttaaa aactgctttg atctattccg ataatatata
     2581 tgcggcacaa gaaacgttga aaatgggtga gaaaaaattt cgtacaggct tagataaatt
     2641 catttttggt gaagaccttg atttgccaat tagtatgaat ccagcacaaa tttctaatga
     2701 agatagcttt aactcagata tcttgctagc tgatactgga tatggacagg gcgaacttct
     2761 aattactcct atccagcaag cagcaatgta ttctgttttt accaacaatg gcacacttgt
     2821 ctatcctaaa ttgattgcag ataaagagac aaaagataag aagaatgtca tcagcgaaac
     2881 agcagtacaa acgatcgtgc cagatctgag agaagttgtg caagatgtaa atggtacagc
     2941 acattctctt tctgctttag ggattccgtt ggcagcgaaa actggtacag cggaaatcaa
     3001 agaaaaacag gatgtaaagg ggcaagagaa cagtttcttg tttgctttca atcttgataa
     3061 ccaaggttat atgatggtca gcatgttgga aaataaagaa gatgatgatt cagcaaccaa
     3121 acgagcatcg gaactattgc aatacctcaa ccaaaattat caataaaaaa ttgcttttta
     3181 agtacagtaa aaggactgtg gcgttatcgt cacagtcctt ttttcaaata aacggttttc
     3241 acgatgtctc tttcacaact tctttttgat ttactcacac ttcataaact ttttttgtaa
     3301 aatatttgag aacaagtgga gaaataagtg tacttaataa aatcacgata actaaaggtg
     3361 aatagtactg tggttcgatc aattgacttt gctgtccaat ttgtaaaatg atcaaagcca
     3421 tttctccacg cgaaatcata ccagcaccga ccatcaatga actatgttga gaaaaaccgg
     3481 caatctgagc accaaaataa cctccaagca atttggtcaa aatcgctaca agcgtcaata
     3541 tagaaataaa gagtagttgt tcactaaagt cagaaaaatc aacttctaat cctacactga
     3601 caaaaaatac tggtataaat actgcatatc ctaaagcctc tacattgtgg tatacttctt
     3661 ctttaacctt tgtctgactg acagctatcc cagcaaaaaa agcaccaatg acagagctaa
     3721 gcccaacaag atccgctacg taagacattc ctaaacaaat aactagcgat acgatgataa
     3781 tagaagaatt tgcgtacact ttttctgcaa atgaaagtag gaaaggcgca atccatttga
     3841 ctaataagaa aataaaaaca aaataaatta gctgttcaat caaaacaagc ggtaaagaag
     3901 cattgctaga agcttccccc gttagaaaag ataggctgaa acttagaact aaaacgacta
     3961 aaatgtcatc taccacagaa gcacccaaaa ttgttgatcc ttcttttgta ttcactacgt
     4021 ttagttcttt taatacttct actgaaatac tgacagaagt tgccgcaaga ataatcccaa
     4081 agaaaatcgc ttcatttccc gaaacttgga atgctttgcc tgtcaaccag ccaaaaacaa
     4141 ccgggaagat gatcccaagc aaagcaacga acataccagg tcggaaatat tttttcaaaa
     4201 gagataaatc actttccaga ccagctaaaa acattaataa gatcacacca atctccgaaa
     4261 agtcatggac tagaatatca ggtttgaccc aattcaatcc agcttggcct agcaaaacac
     4321 caacaagtaa ttgtccaatc actgctggga tacctaatct ccgcgagata tgtgagccaa
     4381 tcgttgttga aatcaatatc aggcataaaa taccaataaa ttccattctc atcattcctt
     4441 tgtataaaat atagcccgaa cttagcataa catacattat tgaaaaaata ggaaaatata
     4501 ctcacaaaaa aaccaacaat ccaaaagatt tttttggatt gttggttttt atacggtcta
     4561 ttttaataac taaaaatgat taacgtatag tcatttttct tattctgatg aaattgataa
     4621 tctcctggat gcccatgaaa atcaaaataa agccgaatat ttggaataga agcatcaaac
     4681 ttttgaatgg attgaacaat aagacgaatc cgccaatcaa caacagaata ctactataaa
     4741 cactccaact tttagctttt gtattcatgc tgaagaacaa ctggaacaag ctgttgatga
     4801 taacagctaa tccaagaaga atcggcagga tagaaacgat tgcaggtgca aaagcataga
     4861 cgatcagagc taagatcagg aaaaaaagac cactgaacag accgatactc caagaacctg
     4921 ttttcctttt aaaacggtaa gcctcaaaaa gattcaacaa cccaagcaaa acaagatagg
     4981 cggtgatcag ataaccgata aaatgaaaaa cagcagaagg gtttagcaca atcgcaatcc
     5041 cagaaataat ataaacagca gctcttaata aggcgtattt ttgaatattt tgtttaatcg
     5101 tctcaaacat ataagaaaac ctcctttgac ttattttaac gtgaactagt cattttacca
     5161 aattaaatgt tacaaaatga gcgaaggaga agaatatctc tggaaaaatc aacttctgag
     5221 gatcttttcc atttttttgc cttttgctag ctcatcgatc attttatcta aataacgaat
     5281 ttctttcatc aagttatcct cgatgtcttc tactcgtaca ccacaaacaa cacctgtaat
     5341 caaggaacgt gaaggattgg ggacaggggc ttccgcaaaa aattgttcga aagtcacttg
     5401 tttttgtagt tgatattcta gttctgtttg ggaataaccc gtcagccaac aaatgatttc
     5461 atctacttcc tcttttatac gatcttttct ctccgctttt gctacataca taggataaac
     5521 gcttgcaaaa cttgtcgtaa aaatacgatt ttgcgccata atcagtcacc tttctttttt
     5581 tcaaattatt tactacttat agtatataag aaaaagagaa gaaaaagcga ttattcaaaa
     5641 aaacaaaaag gattgtgaca aaattcttgt caccatcctt ttttccgctc tctgactttt
     5701 tacaatcttc atagctcaag tgcgcatcag ataatcgaat gctcctaaag ctgcagtagc
     5761 tcctgaaccc attgaaacaa tgatttgttt gtatggacta tctgtacaat caccagctgc
     5821 gaaaacgccg gggatattcg ttgcgccatt tttctcggtc actatttcat ttcttggatt
     5881 catttcgatc gttccatcta gccaatccgt attagggagt aagccgacta gaatgaatac
     5941 tcctgaaaca gctagtgtat gcatctccat cgtacggcga tcgatatagg agagagattc
     6001 tacctgattc gtaccgctga tttctttcgt cgctgcatgt ttgataacag tgacattcgc
     6061 aagagagact agtttatcct gtaagatttg atctgctttt aattctggta agaattctag
     6121 aacatataca tgttttgcta acccagccaa atcgattgct gcttcaacac cagaattacc
     6181 accaccgata acagcaatct cttttccttt aaataaaggg ccgtcacagt gtgggcaata
     6241 agcaattcct ttatttttaa actctttttc gcctggtaca ttgatagaac gccagtgcgc
     6301 acctgtcgcg atgatggcgg tttttgcttt aagtgatata ccgttagcta agccaatatt
     6361 aataaaatgt tgctctttat ttaaggaaac ggcctgttga ttcatcataa tgtcgatagg
     6421 gtaactacgc acatgacttt ctacttgctg catcaatttt ggaccttctg tgtaagaggt
     6481 accaatcata ttttcaatcc ctagtgtttc aacgacttgt ccgccgaaag aatcagcgac
     6541 aagtcctgta cggattcctt tacgtgctgc atagatagct gcactactag ctgctggtcc
     6601 accgccaatt actaatacat cataaggttc tttttcaaac aactcttctt caatcaaagg
     6661 gccagtgatt ttttcaacaa tttgttcgat cgtcatgcgt ccgctactaa attcttctcc
     6721 atttaaaaac acagttggca ctgccatgat ttttttatca tcgatttctt tttggaacat
     6781 cccaccttca atcatcgtat gagtaatatt tggatttaat acagctaata tattcaatgc
     6841 ctgtacaacg tcggggcaat tatgacaagt caagctaaca taactgtcaa aatgcaatgt
     6901 ttgtgttatt tttttgattt taagactcaa agattcactg attttcggtg cccgtccact
     6961 aatttgtaac atagccagta aaaaagagga aaattcatga ccgaggggga taccagcaaa
     7021 aacgattcca ctttctttgc caactggatt gatagaaaaa ctaggtgtgc gcatcgaatc
     7081 tcttttttcc caagagattt taggagacat ttctgctatt tccttcgtaa attcagctag
     7141 tttttgacta ttttcactat tatccaaact aacagaaaaa acaataggag attcaatcag
     7201 tgctaaatat tgttcgagat tttgtttagt ttctttgtct aacatatcca ccacctaaat
     7261 ctttccaaca agatcaaaac ttggtttcaa tgtttcgcca ctttctttcc attttgccgg
     7321 acaaacttca cctgggtggg tacgtacata ttgagcagcg cgaatcttat caatcagtgc
     7381 actagcgtca cgaccgattc catctgcatt gatttccata gcttgaacga ttccgtctgg
     7441 atcaataata aatgttcctc gctgagctaa tccttgctct tcatccaaaa catcaaaaat
     7501 acgagaaatc tgatgagacg gatcaccgat catcacgtat tcgattttac cgatagcatc
     7561 tgatgtatca tgccatgctt tgtgagtaaa gtgggtatcg gtagagcaag aaaagacttc
     7621 aacacctaaa gatttcaatg tactgtattg ttcttgtaag tcttctaatt cagtaggaca
     7681 aacaaaagta aaatcagctg gatagaagca aagaatactc cagttcccca taagatcttt
     7741 ttcactgact tctaaaaatt ctccttgatg ataagcatgt gcattaaatt cttcgatttt
     7801 tttcccaatc aatgacatag taaaacctcc aataataatt atttatttat aattattgta
     7861 atttagttgg agttgcaaag tcaatggata ttttgttatt taataaagat tactcaaaaa
     7921 cgaaggttaa ttataaaaat agaagaggtc gcaagaaaat gctactgctg gaaatactcg
     7981 ggataacgtt gagtaacagt ttcctgttct ttcagcatca tgtgtaagtg caatcccgtt
     8041 tgaaatcgag cttcttctgg ttttttatca actaaagcct tgagtaaatc ataatgttgt
     8101 tccatgatcg tttcccatgg aagcccctga atggtcactc gtaaccagcg gaaacgttct
     8161 aaatgggtat tatggtcatc taaccagttc caaacttctt ttcttcctgc aatttcgaaa
     8221 caagcttcat gaaataaatt atctgcacgg aaaaaagcgc gttcgtcatt tattttgact
     8281 gctttttctt gttcttctaa aatcgtttct agcactattc gtgtttgtgt gtccaatttg
     8341 gaacaacatt caatcatgat ttgctcttcc aactgttctc ggacaaagcg agcattttct
     8401 gcagaaacaa gattaatttt ggaaacatac gtaccacttt gaggtatcgt atacacgagt
     8461 tcttgctgac gtagttgaat caatgcttcg cgaatgggag tccggccgat agcaagatgt
     8521 tcctccagtg ccttttcaga tattttcctt ccaggttcta agtcagcata gataatttgt
     8581 ttacgaatcg cttgataagc ttgtgattgc aagctgcttt tcatctttat tcctccaaaa
     8641 tcatttctat gaacgtagta taacagaaga ggtctgaaaa acaatctggt atgtcacatt
     8701 aactagtaaa tttgtataaa tagtaaaatt ttggttgaca tagcgatttt tattgtataa
     8761 aattcacttg aatacaaaaa ctaatatgtt agtttaggag gggaaaaatg attgatttaa
     8821 acaactggag gaactttatc cctgaattat ctcaaaaaga agtagaaata cctcattttg
     8881 accgtgcatc attaagagaa aatggacaaa aaaatcctgt ctggatccat ttcggtggcg
     8941 gaaatcttta tcgaggattt catgcagaaa tcgctcaacg tttagcggat ctaggcgaat
     9001 tgtccactgg tgtcattgtt tgtgaaacat tcgatgaaga aataattgaa agggcttacc
     9061 aagattttga tgatttgatt cttgaagtgg tcatgccaag cagtggagag ttcgtcaaac
     9121 gtatattagc tgcgacagct gacagtctgt attgtcatcc agattgttct gaacactttg
     9181 ataagatgat tcgacacttt gaaaatccta gtttacaatt agttactttt gctattactg
     9241 aaaaaggtta tcgattgaaa gatacaaata ataaatatat aggaccgatt gcagtcgata
     9301 taaaaaatgg tccaaagaag ccatcacata caatgagtat tcttgctgcg ctactttata
     9361 aacgtttttt atctggagct tggccgattg cacttgtctc tacagataat ttttcacaaa
     9421 acggtagtca attacgtact tctttgctaa cgatcgcaaa agaatggcaa aaggaaggtt
     9481 atgtacaaaa tacatttatc caatacttat cgaatgaaga aattgttgcg tttccatgga
     9541 cgatgattga tcgtatcacc ccgaatcctt ccttgtcagt tagcaaaatg ttggcaaaag
     9601 agggcttttc aaatatgtcg attttccaaa ccgcaaaagg aaataatatt gctccatttg
     9661 tcaatacaga ggacatccat tatttaatca tggaagatca ttttcccaat ggacgtccga
     9721 aattcgaaaa agctggggta ttcttgactg atcgtgcaac ggtagataaa gcagatacga
     9781 tgaaggtgac gacttgcctt aatcctttgc atacgtcttt agctattatg ggttgtttac
     9841 tggggtatag gagcatagct gaggagatga aagatccaga tttatcgctg ctagtttctg
     9901 aaatcgggta caatgaaggc ctcccagttg tggagtctcc aggtatcatt gatccaaaga
     9961 tctttctgga agaagtaatt actgtgcgct tttctaatcc aaatattcct gatatgcctc
    10021 agcgaatcgc cacggatacc tctcaaaagg tggcgattcg ttatggtgag acgattaaaa
    10081 aatatatgga aatagaagtt aaagacccgc agacacttgt attcatacca ttgactctcg
    10141 ctgcttggct tcgatacctt attggcattg atgatcaagg aaaaccattc atcccgagtc
    10201 cagacccact attgacagac tttcagcata agctttccga tattcaactg ggtacgcaag
    10261 atacatcaca tattcatgag aaaatggctc ctattttgtc aaataaagaa atttttggtg
    10321 tagatcttta ccaagcagag cttggcagaa aaatagaaga aatgttcatc aagatggtag
    10381 caggaccgaa tgccgtacga aaaacattac aggattactt acaagaacat ggaggaaatt
    10441 tatcatggaa atgactttta gatggtacgg atcacaggaa gaaaagatta ccttagatga
    10501 aatcagacag attcctgcgg tcaaagggat tgtaggcaca ttgatggata taccagtagg
    10561 agaggtatgg cccagggaga ggatcaaaga actaaaagaa gaaattgagt ctcgtgggtt
    10621 aacactaaaa gtaattgaaa gcgtcaatgt gcatgatgcc attaaaattg gattaccttc
    10681 ccgtgagcag tatatcgaaa actataagca gacaatccga aacttggcag aatatggaat
    10741 agaagtcatc tgctataatt ttatgcctgt atttgactgg gtgaaatctg accttgacta
    10801 ccgcttagaa gacggctcat ccacgttagc ttttatatct gatgatattc cagatgatcc
    10861 aagagaaatc gtcgcgcgca tcgaacattc cagcaatgaa tttgaattgc cgggatggga
    10921 acctgagcgc ttagctcata tcaaatcctt gcttgaagcc tatgcatcag tagacgaaga
    10981 aaaacttcga gaaaattttg cctattttct ccaatcaatc atcccaacat gtgaagaggt
    11041 aggggtcaaa atggctgtac atccagatga tccgccatac tcgatttttg gattacctcg
    11101 aatcgtaaag aatcgagaag atttagattg gttgtgccgc atagtagaca gcccttataa
    11161 cggaatcact ctttgtacag ggagtattgc agaagatcca gaaaatgatg tatatgggat
    11221 tttagcagaa ttttgtcaaa gagatcgaat cccatttgca catgttcgaa atattaaatt
    11281 tttagctaat cgagatttct atgaggcacc gcataaatcc gaatacggtt cattagacat
    11341 gtaccgcata ctaaaaacaa tgtatgacaa cggatttaac gaatacatcc gtcctgatca
    11401 tggaagaatg atctggaatg agacaggaag accaggatac ggcttatatg atcgagcatt
    11461 aggtgctgtt tatctaaatg gattatggga agcaatcgaa aaaggaaaca aaagtaaaga
    11521 taattagtga aaaaaactgt aattttatta tgtaaataag aaggatgttc aaattcttga
    11581 tacataaatc cggaggttgt taataagcat tcggatttat gtatcttatt tggtctagga
    11641 agtaacttga ttttcttttt tcgattgatc catgagtgct tcttctttta attcaaagat
    11701 atattctgag atcctcataa tcactttttt ccagtcttct ggtgtgatga ttccttttaa
    11761 aatatagatt tttttgatat gagaaggcaa ttgtttcaaa gggcagtcgc tgataacgat
    11821 gttgcagttc tccataaaat cattttgtat tttgatcgat tcatttgaaa aaatttgttc
    11881 tatcttaacg atcagatctt tactgatata aaaatcacgc gtatagttca caccaatcac
    11941 aattcgcggc tgttttttgt agcgatcata caaatgtact aggtcttcaa ccatccattg
    12001 cacattttcc ttttctaaaa taactggtaa caagtgttga ttttgttctc tgaaatccat
    12061 ataaaattgt tcaatggcgg tttggacttc tttcaacacg ctatgttctt gataaagcat
    12121 gaaagtagga aggggcgtat ttggttgatt gaagttaaga aatccattat aaagtttatt
    12181 tcgtgcgaat gccaatgagt aaagaatctt ttctttttca ggtataaaca aatcaaatac
    12241 agtacaaaag gactccacca tttctcttga ataaagaaca tgaggaacgt ttgctttttt
    12301 taaaagtttg tattgctcca agcttgtttc ttccgattcg attttcgtga tagaaagtcg
    12361 agctaaaata ttcactaaga tgcgttgttc gcgacaaatt ggcagattag gtcgcaaaat
    12421 atctacagag ggatcaataa aaaagtcaat atattcatac tctgtgaatt ctttttgtac
    12481 gtctttcaca gaagtt
//
LOCUS       DAYYGO010000081        12435 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000081 DAYYGO010000000
VERSION     DAYYGO010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12435
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.083"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      complement(19..85)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(127..774)
                     /locus_tag="ACHA5T_001974"
     CDS             complement(127..774)
                     /locus_tag="ACHA5T_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HFM8887538.1"
                     /translation="MEQTIKPYQYFFIKREVDQLLNAYSSVNDPKTVQTVQALAAEKI
                     RDILEHELPEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMVYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     gene            complement(1053..1556)
                     /locus_tag="ACHA5T_001975"
     CDS             complement(1053..1556)
                     /locus_tag="ACHA5T_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8887539.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(1679..2443)
                     /locus_tag="ACHA5T_001976"
     CDS             complement(1679..2443)
                     /locus_tag="ACHA5T_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFM8887540.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     NAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLADVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EISSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            2550..2825
                     /locus_tag="ACHA5T_001977"
     CDS             2550..2825
                     /locus_tag="ACHA5T_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFM8887541.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            3502..4452
                     /gene="yidC"
                     /locus_tag="ACHA5T_001978"
     CDS             3502..4452
                     /gene="yidC"
                     /locus_tag="ACHA5T_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFM8887542.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(4631..5605)
                     /gene="lacD"
                     /locus_tag="ACHA5T_001979"
     CDS             complement(4631..5605)
                     /gene="lacD"
                     /locus_tag="ACHA5T_001979"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFM8887543.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDTKIPGR
                     LPDLLNIWSVKRLKEAGADACKFLLYYDVDEPAEINVQKQIVIERIGSECEAEGIPFF
                     LEILSYDATIGDPSSLAYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QETCYSQAEASAYFYEQSKVTEQPFIFLSAGVNATLFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATPVYVDR"
     gene            complement(5602..6060)
                     /locus_tag="ACHA5T_001980"
     CDS             complement(5602..6060)
                     /locus_tag="ACHA5T_001980"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFM8887544.1"
                     /translation="MTIIHEENIFLQQPLDSQEEAFKFLAKQTFCLGIALDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSMIIATLNKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRKAKSKTEIATILTKEIGEDL"
     gene            complement(6057..7520)
                     /locus_tag="ACHA5T_001981"
     CDS             complement(6057..7520)
                     /locus_tag="ACHA5T_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFM8887545.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGIKIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFAGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAMSGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSFDPDHAQYNATAAMVKAVGDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLTILPKQLDGLKAIFLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEHSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLAQGN
                     TTFMAGVSAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKMDNKAANRTVIEKGRAGDLS"
     gene            complement(7502..8452)
                     /gene="pfkB"
                     /locus_tag="ACHA5T_001982"
     CDS             complement(7502..8452)
                     /gene="pfkB"
                     /locus_tag="ACHA5T_001982"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFM8887546.1"
                     /translation="MSIYTCTMNLAIDLFIETEEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPKIPQTAVHNFLNQIRALQAEDYLCVSGSLPRGLSPKVLIEISRICQEKGI
                     HLIIDSSDQEAMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVELGAENV
                     LLSLGEKGAILFTKDRVFRGNSPKGKVVNTACAGDTMLGAFLAGYMNRRPLDETLRKS
                     IAAGSSTAFRKGLTDFLDVEELSKQIKIKEERFETWENIN"
     gene            8595..9344
                     /locus_tag="ACHA5T_001983"
     CDS             8595..9344
                     /locus_tag="ACHA5T_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8887547.1"
                     /translation="MDHKLSETEQYLWNFIEHHILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGETKELVTAAKNCYDAEIGSILVTANHSSTLKDYCEINLVGFKSEGS
                     YFPDYEVRSRLPLQIIARILLDAYVLRMGEN"
     gene            9611..11089
                     /locus_tag="ACHA5T_001984"
     CDS             9611..11089
                     /locus_tag="ACHA5T_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFM8887548.1"
                     /translation="MKKLSQKRLAQIIIDSRKSLKMTQQQLAVATGINRALISRLESL
                     DFIPSIQQLESLAEVLNFEPTDLFINESNKSNRTKHQSKNIAVAGTGYVGLSIATLLA
                     QHNQVTAVDILPEKVKQINNKKSPIQDDYIEKYLAEKNLNLIATTDGAAAYREADFVV
                     IAAPTNYDSKQNYFDTSAVESVIKLVLEVNPKAIMVIKSTVPVGYTESIRKKYNTKNI
                     LFSPEFLRESKALYDNLYPSRIIVGTDQTDKTLREASHTFADLLQEGAIKKDIETLFM
                     GFTEAEAVKLFANTYLALRVSYFNELDTYAEMKGLNTQEIIHGVCLDPRIGTHYNNPS
                     FGYGGYCLPKDTKQLLTNYNDVPQNMISAIVDSNRTRKDFIADRVLELAGTYEANSSW
                     NAEKEKEVVIGVYRLTMKSNSDNFRQSSIQGVMKRIKAKGATIVIYEPTLENNTTFFG
                     SKVINNLDDFKNISHSIIANRYDSNLDDVKEKVYTRDLFQRD"
     gene            complement(11157..12248)
                     /locus_tag="ACHA5T_001985"
     CDS             complement(11157..12248)
                     /locus_tag="ACHA5T_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFM8887549.1"
                     /translation="MLELNTSLSNRTILITGAAGFIGSNLVLELLRTQSSIKIIGIDN
                     MNDYYDVSIKNYRLSEIETEAKNHPGSTWNFIKGSIADKATIDFIFDNYKPSIVVNLA
                     AQAGVRYSITNPDMYIESNIIGFYNILEACRHSYDNGESGVEHLVYASSSSVYGTNKK
                     IPYSTEDKVDNQVSLYAATKKSNESMAHAYSKLYNIPSTGLRFFTVYGPAGRPDMAYF
                     GFTDKLRAGDNIQIFNYGNCKRDFTYIDDIVEGVKRVMQCAPEKIDGEDGLPIPPYHI
                     YNIGNNTPENLLDFVDILQQELIAAGVLPEDYDFESHTELVPMQPGDVPVTYADTSAL
                     ERDFEFKPSTSLRDGLRKFAQWYKSFYEV"
BASE COUNT         3869 a         2526 c         2103 g         3937 t
ORIGIN      
        1 gaaaggggct atccccagac tttagtaggc gtcattagct atctttcgtt agcatttaaa
       61 ggcgaacacc atcacctatt cacttccatt aaaagatagc atatttttct gtaaaaaaca
      121 ataaaattat cgttctttct ttacacgttc agcaaattca tagaaaggag ccaatttcgt
      181 taactgttga ttacattggt cactatcatg acaaatataa ttcccctttt tcgtatacgt
      241 gccatcagaa cctgctttcg ttgtagaaag aaaaagcgac acattcgatg ttttctgaca
      301 gattgcacag attcccttat ttatcgttgg tgataaagta ccgctaatcc cttttaattt
      361 ttcatcttcg taatagacca tgaatttttt ctgcgtacct ggatcattcc agccgatgta
      421 cgtatgttct tgcaagtcga tttcattcca agctggttgt ttgagttttt tcacttttcg
      481 gaataatttc tcgatttgtg gtttgctagg ctgcataaac ggctggacga tttgcttcag
      541 ctcctccaat gctctttctg cttgcgcttt tgtcagtttt acgtctaaaa ctgtcgttaa
      601 aaatgtatcg atttctggca gttcgtgttc taatatgtct cgaatctttt cagctgctag
      661 tgcctgaact gtttgtaccg tttttggatc attgacagat gagtacgcat ttaataactg
      721 atctacttct ctttttataa aaaaatattg gtatggtttt attgtttgtt ccatgatttt
      781 aaagtccttt cttctaacca aagtgcatac tttgtagaaa aaagtgatct tctgcaaagt
      841 atgtgttacc tgaagatccc ggactctttt aaataagata cgttttgcat cttatcatca
      901 ccccctttaa tgttcattct tctatgtaca tgattcattc gataaataaa agtaaaccgt
      961 atcttgaaca gatagtcaac tgcaaacaga aaaaaccgag aaaatcagcc agattttctc
     1021 ggaatatagt ctattattat tggttttacc tattaaatcg aggattcttt cccaaagtaa
     1081 cgttgccatc tcttacgcat acttgcagac atgggctgag ccgcttcttt gactgcgcag
     1141 tatttgtcca cgaatgtcac aatatatcct tctttatact taggcggtgc gatcgttgca
     1201 ccccacatgt gcttcaaaat gatatctcgc tcaagatccg acaaatcggt gattttttct
     1261 gcattcttta cagcaattct tggatggata tacgcatgtg tcccctcgtc aaatttcgtc
     1321 gtacgccaat catagtagaa cagatcatgc aaaagtccag cacgcgctgt tgctttagca
     1381 ttgccacccc atttttttgc gagcagataa ctatagtaag aaacactgat ggaatgctcc
     1441 aaacgagtgg aatgaacatg ctgtgtgtaa ttcgctaatt ttttcactgc ttctgtttct
     1501 aaaagatctt gtacataaga catatattct tcgtcttcac gccatttttc tgtcataatt
     1561 atcgatctgc cacctttttg aatttcattt tcttctgaaa ttgatctaag tataccacca
     1621 gtgttcggat tattctagtc gctctagata aaatagctaa agcttaatag ttccttaatc
     1681 ataaagaaaa atgataaaga acgatccctg ctgcgactcc gacattcaat gattccgcat
     1741 ttcctttcat tgggatatat acgttctgat cagtcaaaga cgagatttcc tgacttactc
     1801 cctgaccttc gtttcccatg attatagcga aatcctctgt tttttctact ttgtctaatg
     1861 catgtgcttc ttcatttaat tctgtaccgt acaccgtaaa ccctttgcct ttcattacag
     1921 ggattacatc tgccaaatct ttttgtaaca caggcaaatg ataattgctt ccctgcatgc
     1981 ttcgcagcac ctttgtacta taaatatctg ccgttccttt acctaataaa acaccagcca
     2041 taccaaacgc atctgccgta cggatcatcg ttcccacatt tccaggatcc tgtacattat
     2101 ctagtaaaag ccagcctcct gaaaaatctg gcgtatcttc agatgttaac gtcatcactg
     2161 ctaagatccc ttgcggtgta ggtagatctg acatagcggt catgacctca tctgaaacta
     2221 gaaaagtaga aatctctttg tttacagcca gccagtctcc ccattctttc atacctctct
     2281 gtgtcacaaa aagttctttt atttctgcat tagacgacgc agcttcttcg atcagatgga
     2341 acccttccaa aagatattct gccttttctt ctctgtattt ctttttatgt agttttttta
     2401 attctttgat tcttggattt tttgctgatt ggatctcttt cacattcctc acctcaactg
     2461 attatatcat ggaataggtg cttgagcttg tttcatccta aaaaaatcgg tatcattgat
     2521 ttataaaaca ggaaagaaag gtgacgaaca tgcgcaaagt taaaatgaat gtccaaggac
     2581 gtgtccaggg cgtgggattt cgctatatga ccaagatggt tgctgatcag ttaggcgtga
     2641 ccggaacggt aaaaaacgaa gacgacggct cagtcacaat cgaagcaatc ggaaatgacg
     2701 acatcatcca aaaatttatc gaagaagtca agaagtctcc gagcccaagt ggacgagtgc
     2761 agtatgtgga tgtccaagaa gatccgatga ttgaggaacg aaagaaattt gatgtagtgg
     2821 gataaggttg tgaaaaggtc gcttagtttt gagtattaag gaaaattttg tgaaaatggc
     2881 tttccacatt tttgcataaa tttgacttaa taccgagaaa ctgacttttg aacaccgtcg
     2941 ataaggttgt ttttgaagcg ctttgcctcg agtattaagg agaaacattt tcaaaatagc
     3001 ttctcatatt ttgaaatgta atgacttaat accgagaggc agctgataaa acaccgtcaa
     3061 taaggttgtg aaaaagctgg tctgcatcaa gcattaagga ctggttgtga taaatagctc
     3121 tccatatttt tcaacaggct ggacttaatg ccgtagatgc aagcttttga acaccgtcga
     3181 taaggttgtt tttgaggcgc tttgcctcaa gtattaaaga caggtagtaa aaaagctgat
     3241 ctataacttg caatgaattt gcttaaccca tattttccag acagaacaaa aaagttttta
     3301 gttttttaaa taagaaagaa acacttggaa tcactctttt ctttctaagt atttctttct
     3361 tgattttttg tcttttttca aaatttttcg ctacgaaaaa gaacgaatat ttatatgaaa
     3421 tttctatgaa gttgattgaa ttccatgcgc ttttttagta tagttaagtt tgtgtaaaaa
     3481 ttatagaaga ggaagtattc tatgaataaa tggaaaaact ggttattggg atccggactt
     3541 gtcagtatcc tgctcttttt atctggctgt gtgcggatgg attcaaacgg aaaccctgac
     3601 acttcaggaa tcgtttatcg agtactcgtc catcctatgg gccaagcgat tacatatcta
     3661 gttgagaatt tcaattggtc ttacggctgg gcagttattg caatgactgt gatcgttcgt
     3721 atcatcatct tgccactagg tatcagtcaa tcgaaaaaaa caatgatcca atctgaaaaa
     3781 atgcaagcgt taaaaccaca agttgaagca gcacagcaaa agctaaaagc tgcaacaaca
     3841 cgtgaagaac aaatggcagc acaagcagaa atgcagcaag tatatcgcga aaatggattg
     3901 agtatgaccg gcggaatcgg ctgtctgcct ctattgatcc aaatgccgat cttctctgcc
     3961 ttgtatttta cggcacgcta cacagaaggt atccgggaat catcttttta cggcatcgat
     4021 ctaggctcac caagtatggt gcttgtggcc atcgcagggg tcgcttatct gcttcaagga
     4081 tatatttcaa caatcggtat tcctgaagaa cagaaaaaga cgatgcgtac gatgctgatc
     4141 gtctctcctg ctatgatcgt cttcatgtca atcagtgcgc cagccggtgt tacactttac
     4201 tgggtagtcg gtggtgtgtt cagctgtctg caaacattca tcacaaacgt tatgatgaaa
     4261 ccacgtttga aagcacaagt ggcagaagag ttgaaacaaa acccgccaaa acaagtcgtc
     4321 actccaagaa aagacgtgac agaatcagca aagccagcta acaacaaaca agcaacaaaa
     4381 caaatcaaac aaccaaaaaa cagtacaaac aacaaaggcc gcaacgcagg aaagcagcag
     4441 aagcggaagt agatagtaaa taagagtgta ataaaaattc attaacagtt ttagcaatta
     4501 aaaatatcga ccaagatttt tgaaaaagaa atctggtcga tatttttatt taagtgaatg
     4561 tttaatttga atcatcgatt gatcgctatc taattgcagc agcatctttt tatgaagaaa
     4621 caactttttc ttatctatca acgtagacag gtgttgctgt ttcttctaat agtgtactta
     4681 attctttaat attttgtttt ccttcttcta acatccaact actcgctttt tcttcgcctt
     4741 cggtaacaaa aggtgccact cccttcgccc atgttgctcg cccacataag acaccattga
     4801 atgtcgaacc tgattgtttt gcaaaacgta atgtatcttg aaatagcgta gcattgactc
     4861 ccgcacttaa aaaaataaac ggttgttcag tcactttcga ttgttcatag aaatacgcgg
     4921 atgcctctgc ttgactataa caagtctctt gcccatatcc ttccacaaat ctcatattca
     4981 ctggcacttc tactttcaat acatccactt gataacgttc ttttgaaaat tctcgcatgg
     5041 cttcaataac tttatgtggt tttacttttg cataggcaag agaagacgga tcgccaatag
     5101 ttgcatcata agaaagaatc tctaaaaaga aaggtatgcc ttctgcttcg cattctgaac
     5161 caatccgttc tataactatt tgtttttgca cattaatctc tgctggttca tcaacgtcat
     5221 agtacaataa aaatttacaa gcatccgcac cagcttcttt taaccttttc accgaccaaa
     5281 tattcaataa atcaggtaat cttcccggta ttttcgtatc atagccggtt ttttcataag
     5341 ctagcagtaa gccacattct ttctctcgaa gcgctgctgc aggcaggccg tattctggat
     5401 caagtaaaat agctgaggca tagggagtca ggtattgaga aaccatctgt ttaaattgga
     5461 tgatgtcttc tgatgtagct tctttatatt ggttgatcat tttcttcaaa gcaccacgct
     5521 ggtcgattgc taatgcgcta ataattccag cttcatccgt caatcgcttc aaacgttcct
     5581 gtttttctat actcatcttc ttcatagatc ttcacctatt tctttagtta atattgtggc
     5641 tatttctgtt tttgattttg cttttctcaa ctgttctgta acctcttttc gcatcaatga
     5701 tcgtgcaacg agagacaata atttcaaatg agtcgttcct gcttctgaat ctggaataaa
     5761 taacgcaaaa ataaaatcaa ctgactgtct atctaaactg ttccattcaa ttcctttgtt
     5821 cagtgtggcg ataatcatac taggttcgtt gattgtttga gacttcgcat gagggatagc
     5881 aaaacctgac attattcctg tagtcccttc tttttctctt tctagaagtt tttgatatac
     5941 ttctttttcg tcaagagcga tccctaaaca gaatgtttgc tttgctaaaa acttaaatgc
     6001 ttcttcttga ctatccaatg gctgttgtaa aaaaatgttc tcttcatgaa tgattgtcat
     6061 gataagtctc ctgctcttcc tttttcgata actgtacgat ttgctgcttt attatccatc
     6121 ttcttgtctt tatcagttac tcttttcttt tggattaatc ctagtatcac tccaccaacg
     6181 atcgaaccaa taagaatcgc tgtaacccat tggatttttc ccgtcactac aggtaacact
     6241 aaaaaaccac catgtggtgc tggaacttgg acgccaaaca aataagttaa gacagcagca
     6301 attgatgagc ctaacatcat cgtgggtaac actcggatcg ggtctttcgc agcaaacgga
     6361 atagctcctt ccgtaatatg agttgatcct aaaataaaat taaccaaacc tgcattacga
     6421 tcattggtat caaaatattt accaaacaat aaagtagcaa aaccagtaat caacggtggt
     6481 gcaatacaag ctgctgacac accagccata aaagtcgtgt ttccttgtgc caaaagagct
     6541 gttcctgtta catatgccgc cttattaacc ggtcctccca tatcaaacgc acacatacag
     6601 ccaacaatca cgccaagaac aagtggattc gaatgttcaa atccagctaa aaagctcatc
     6661 attccttcat ttatagcctt cattggacct gccagcaaag acatcacgat gccaataaat
     6721 gctacaccaa taactggata taagaagatt gccttcaagc catctagttg tttaggtaaa
     6781 attgtcagta gcttttgcaa aaataagata aaataacctg ctaagaaccc agctaaaata
     6841 cctcctaaaa atccagtacc gccattcatg gctacgattc caccaacaaa tccaacgact
     6901 aaccctggac gtttcgcgat cccttctgca atataagctg ctaaaactgg aaccatcatt
     6961 cccattgctg catcgccaac tgcttttacc attgccgctg tagcattgta ttgtgcatga
     7021 tctggatcaa aagaatagat tccccaaacg gcaaatgaaa tagcaatcaa tactccgcca
     7081 gctaccacga aaggcaacat atgagagaca ccgttcatta aatttttata gatgctacga
     7141 ccaaaattag caccactcat cgcttctggt tctaaagtat cagcagcttc attatctgct
     7201 aagacagtac cttttcctgc caatgcttcg tcaatcagcc gttgtgcatc cttgattcct
     7261 gtactgacag aaacatcaat gactcgtttt cccgcaaatc tttctgaatg gacatcttta
     7321 tctgcggcaa taatgacagc atctgcctgt ttgatttctt cagccgttaa ttcattttcg
     7381 atcccaattt gtccatgggt ctccactttg atcttgatgc cttttttcct agctgcttgt
     7441 tctaatgcct cttgcgccat gtaagtatgc gcaattcctg tcggacaacc ggttgctgca
     7501 attagttgat attttcccat gtttcaaacc tctcttcctt gattttgatt tgtttactta
     7561 attcctccac atccaaaaaa tcagtcagtc ctttacgaaa agcggttgag ctgcctgctg
     7621 ctatgctttt acgtaacgtt tcatcaagcg gtcgtcgatt catatatcct gctaaaaaag
     7681 cgcctaacat ggtatcacct gcacatgctg tattgacaac ttttcctttt ggcgagtttc
     7741 ctcgaaacac cctgtctttc gtaaaaagaa tcgctccttt ctcaccgagg gataacaaaa
     7801 cattttctgc acctaactct actagtcgtt gtccataaac aaagtaatct tcatctgtaa
     7861 ccatttttcg tccgaaccaa gaagaaagtt cttcctcgtt cggtttcagc aaaaacggtc
     7921 gatagggtaa acaatccatg gcttcttgat cactactatc aataatcaaa tgaatcccct
     7981 tttcctgaca aatccgactg atttctatta aaacctttgg agacagccct cttggtaaac
     8041 tcccagaaac acaaagataa tcttctgctt gtaatgctct tatttgattt aaaaaattat
     8101 ggacagctgt ctgcggtatt ttaggtcctt gattcactaa tttgtattct tctcccgttt
     8161 gattgacttg tgtaaataca ttgatacgcg tcataccagg cacttcaata aactctgttt
     8221 ggatttgttt ctgctgtaaa tagtcttcta tatactttcc tgtaaaccca gcagaaaaac
     8281 ctaatgccgt acttgggaaa tctagcattt ttaatactag tgacacattc accccttttc
     8341 cgtttgcctg aatgtcatcc tcttttgtgc ggtttaccat aaaaggatgc atctcctctg
     8401 tttcaataaa tagatcaatt gccaaattca tcgtgcatgt gtagatagac ataaccaacg
     8461 acctccttga ctctattatg aaatacgcga aagagaaaat gttttcttaa tcgtcccaac
     8521 acaaagaaaa cctttacatt tataaactcg tcgtgctatt ttttaactaa cttttggtga
     8581 aagaaggaaa gaatatggat cacaaattaa gtgaaacaga acagtacctc tggaacttta
     8641 tcgaacacca catcctagag atccctaact actccatcgt gaaactaagt gaacaagcaa
     8701 atgtctcaac agccaccatt gtgcggacaa tgaaaaagaa aggctatgaa ggatttactt
     8761 cgtttaagca ccatctaaaa gaaggagaaa acaagaacat caatttttct ttcttagata
     8821 aagtagacaa aggtatccgt cgagccattc taaaaaatga acaagaagtg gtacgtacga
     8881 tcaatatgtt ggaaatcgga aatattgagg atgcaataca aagaatcaaa tttgctgacc
     8941 gggtttttgt ctttgcacga ggattttcag aaatgattgg tcaagagatg ctcgtaaaac
     9001 ttcaattaac agggaaaaat tgtcaaatgc atactgatcc agaaatcatc aaaagcatca
     9061 gtcggaaact aactaagaaa gacagcgtta tttttgtttc tttgaacggc gagacaaaag
     9121 aactcgtcac cgccgcaaag aattgctatg atgcagagat tggttcgatt ctagttacag
     9181 ccaatcattc ctctacactg aaagactatt gcgagatcaa tctcgtaggt ttcaaatcag
     9241 aaggttctta tttccctgat tatgaagtac ggtcacgttt gcctctccag attatagcga
     9301 gaatcctgct tgacgcctat gtattgcgta tgggggagaa ttgaaaagca gtttattatg
     9361 gttggttaca gtttaaaaca tcacattttt tattctttat attgtatgga ctcacggaaa
     9421 ctttttatgt ataaagttat tcacaatcta taaagttact ttaaataaca aaaaaataat
     9481 tgaaaatact taaaatcaaa aaatatcttt ttaatcaata aaaaaattga cagttttatt
     9541 aataataagc tattctgtta tagaagaaaa aattgtttgt tattattagc aacaaaaaaa
     9601 ggagtgcatc atgaaaaagt tatctcaaaa acgtttagct caaatcatta ttgattctag
     9661 aaaatctctg aaaatgacac aacaacaact tgcagtagct actggtatca accgtgcctt
     9721 aattagtcga ttagaatctc tcgattttat cccgtcaata caacaattag aatcattagc
     9781 agaagttcta aattttgaac caacagatct atttataaat gaatcaaata aatcaaacag
     9841 aacaaaacat cagtcgaaga atattgctgt agccggtact ggttacgtag gtctttccat
     9901 tgctactctt cttgcccagc acaatcaggt aacagcagta gacattcttc ctgaaaaagt
     9961 aaaacaaatt aacaataaga aatctccaat acaagacgat tacattgaaa aatatctagc
    10021 tgaaaaaaat cttaatttaa ttgcaacgac tgatggtgca gccgcctata gagaagccga
    10081 ttttgttgtc attgctgctc caacaaatta tgatagtaaa caaaattatt ttgatacttc
    10141 tgctgtagag tcagtaatca aattggtgtt agaagtcaat ccaaaggcaa taatggtcat
    10201 caagtcgacg gtacccgtag gctataccga aagtattcgt aaaaaataca atactaaaaa
    10261 tattctattc agcccagaat ttctcagaga atctaaggca ttatatgaca atctatatcc
    10321 ttcgcgaatt attgtcggca cggaccaaac tgacaaaact ttacgagaag cctctcacac
    10381 ttttgcagat ttactccaag aaggagcaat caaaaaagat atcgaaactc ttttcatggg
    10441 attcacagaa gctgaagcag ttaaattatt cgctaatact taccttgcac ttcgagtatc
    10501 ttacttcaat gagctggata cttatgcaga aatgaaagga ctaaatacac aagaaatcat
    10561 tcacggggtt tgcttagatc caagaattgg cacacattat aataatccat cttttggata
    10621 tggaggctat tgtttaccga aagatacaaa acaacttctc actaactata acgatgttcc
    10681 tcagaatatg ataagtgcaa ttgtcgatag taatcgtaca agaaaagact ttatagcaga
    10741 tcgagtgtta gaacttgctg gcacgtatga ggctaacagt tcgtggaatg cagaaaaaga
    10801 aaaagaggta gtcataggtg tataccgtct tacaatgaag agtaattcag acaacttccg
    10861 tcagtcatct attcaaggag ttatgaagcg aattaaagca aagggggcaa ctattgtaat
    10921 ttatgaacca acacttgaaa ataacactac attttttggt tcaaaagtaa taaataattt
    10981 agatgatttt aaaaatatca gtcacagtat tattgctaat cgttacgata gtaatcttga
    11041 tgatgtaaaa gaaaaagtat ataccagaga tttatttcaa cgagattaaa aaataacacc
    11101 tcagatttgt agtgtctgag gtgtttctat aaacttttat aagtaaaaaa atacaattat
    11161 acttcataaa aagacttata ccactgcgca aatttacgaa gcccgtcacg caaacttgta
    11221 cttggtttaa attcaaaatc tcgttctaac gcactagtat ctgcataagt aactggcaca
    11281 tctcctggtt gcattggaac tagttcggta tgagattcaa agtcgtaatc ttctggtagc
    11341 acccctgctg caattagctc ttgttgtaga atatctacaa aatctagtag gttttccggc
    11401 gtattgttac caatattata aatatgatat ggtgggattg gtagcccatc ttccccatca
    11461 attttctctg gagcgcactg cataaccctc tttactcctt caacaatatc atctatatag
    11521 gtaaagtcac gcttgcaatt cccataattg aatatttgga tattatcacc agcacgaagt
    11581 ttatcagtaa aaccaaaata agccatatct ggacgcccag caggaccata tactgtaaag
    11641 aaacgtaacc ctgtagacgg aatattgtaa agtttagaat atgcatgtgc cattgattca
    11701 ttacttttct tagttgctgc atacaacgat acttgattgt ctactttatc ctctgtagaa
    11761 taaggaattt tcttattagt accataaacg gaagacgatg aggcatacac taaatgttcc
    11821 acaccagatt ctccattatc atatgaatga cgacaagctt caagaatatt atagaatcct
    11881 attatgttag attcgatata catatctgga ttagtaattg aataacggac tcctgcctga
    11941 gctgctaaat tcaccacgat acttggttta taattatcaa agatgaaatc aattgttgct
    12001 ttatcagcaa tagaaccttt gataaaattc caagtagatc ctggatggtt ctttgcctcc
    12061 gtttctatct ctgataatct atagttctta atagagacat catagtaatc attcatattg
    12121 tcaatgccaa tgatttttat tgatgactga gtacgtaata actcaagaac caaatttgat
    12181 ccaataaaac cggctgcgcc agttattaat attgttctat ttgataagga cgtgtttaat
    12241 tccagcattt tttagcactc actttctttt tttcttatga atacatattt gttatatatt
    12301 aaaaaatctt atttagactt atctattgtc acttttatca ttctaatctt tgcacgtagt
    12361 agaataatta tacagatagg aaattgctaa gcaacccact tctttctatt atctattaac
    12421 ttattctaaa acgaa
//
LOCUS       DAYYGO010000082        12223 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000082 DAYYGO010000000
VERSION     DAYYGO010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.084"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            74..1249
                     /gene="mltG"
                     /locus_tag="ACHA5T_001986"
     CDS             74..1249
                     /gene="mltG"
                     /locus_tag="ACHA5T_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFM8887550.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            1385..1864
                     /gene="greA"
                     /locus_tag="ACHA5T_001987"
     CDS             1385..1864
                     /gene="greA"
                     /locus_tag="ACHA5T_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFM8887551.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            2033..2764
                     /gene="liaF"
                     /locus_tag="ACHA5T_001988"
     CDS             2033..2764
                     /gene="liaF"
                     /locus_tag="ACHA5T_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFM8887552.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            2761..3828
                     /gene="liaS"
                     /locus_tag="ACHA5T_001989"
     CDS             2761..3828
                     /gene="liaS"
                     /locus_tag="ACHA5T_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFM8887553.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            3835..4467
                     /locus_tag="ACHA5T_001990"
     CDS             3835..4467
                     /locus_tag="ACHA5T_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8887554.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            4607..5272
                     /locus_tag="ACHA5T_001991"
     CDS             4607..5272
                     /locus_tag="ACHA5T_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFM8887555.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            5300..5593
                     /locus_tag="ACHA5T_001992"
     CDS             5300..5593
                     /locus_tag="ACHA5T_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFM8887556.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(5661..6608)
                     /locus_tag="ACHA5T_001993"
     CDS             complement(5661..6608)
                     /locus_tag="ACHA5T_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFM8887557.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(6786..7589)
                     /locus_tag="ACHA5T_001994"
     CDS             complement(6786..7589)
                     /locus_tag="ACHA5T_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFM8887558.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            7735..8649
                     /locus_tag="ACHA5T_001995"
     CDS             7735..8649
                     /locus_tag="ACHA5T_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM8887559.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            8697..9167
                     /locus_tag="ACHA5T_001996"
     CDS             8697..9167
                     /locus_tag="ACHA5T_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFM8887560.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            9252..9860
                     /locus_tag="ACHA5T_001997"
     CDS             9252..9860
                     /locus_tag="ACHA5T_001997"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFM8887561.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            9994..10194
                     /locus_tag="ACHA5T_001998"
     CDS             9994..10194
                     /locus_tag="ACHA5T_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8887562.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            10948..12174
                     /locus_tag="ACHA5T_001999"
     CDS             10948..12174
                     /locus_tag="ACHA5T_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM8887563.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
BASE COUNT         4208 a         1860 c         2513 g         3642 t
ORIGIN      
        1 aaatcattca cgccaattca aatatcatgt ataatgaata aagttacgaa cttgatagta
       61 aggaggagtt gttttgtcag gaaacaaaca acacccaagt aaagataaaa aagaagccag
      121 taaggcagct acagcagata taatcgacaa gcggagaaat ctgcgtaaaa aagaagataa
      181 gattgtgagg aagatcattt tagtgattgc tcttacatta ttgattattg gcggatttct
      241 tggatttacc gtgtatcgtt atgtagatag cgggttgaag cctttagata aatcagatga
      301 ccaattagtc caagtcgaga tcccgagtgg ctcttctaac aagcagattg gtgagatttt
      361 ggagaaggac aatattatta aaagcgggat tgttttcaat tattatacaa aatttaaaaa
      421 tttaactggc tttcaagctg gctattatca gttagcgcct aatatgactc ttgacgagat
      481 tggcaaacag ttgcaagaag gcggtacttc tgagccaaca aaagttgcag atgggaaaat
      541 tgccattcca gaagggtacg atatcgatca gattgctgaa cgggtagcga aggtaactgg
      601 taaagacaag aaagaatttt tagatttagt gaatgatgag actttcttta acgagttgca
      661 ccaaaaattc ccagaactgt tggaaagtgc atctaaagct gagaacacaa aataccgttt
      721 ggaaggttac ttattcccag ccacatatga ttattacacg agtacttcgc ttaaggatct
      781 agtgattgaa atggtgaaca agacgaatac ggtcatgcaa aactattatt cagctatcaa
      841 gcagaaaaat ttgactgtac aagaagtttt aactctagct tctctagttg aaaaagaagg
      901 cgtaaaagaa aatgatcgta aaaacattgc acaagtcttc tttaaccgaa tcaaagcaaa
      961 tatgccactt cagtcagata tctctgtttt atacgcatta ggtgaacaca aagaattagt
     1021 gacgtatgaa gatacagctg tagattcacc atataatttg tataccaata ccggctatgg
     1081 acctggacct tttgacaatc caagcgaaga ggcaatcaag gcagtattag aaccagcaga
     1141 aaatgactat tattatttcg tagcagatac gagcactgga aatgtttatt tcgctaaaac
     1201 ttatgaagaa catatggaat tagtggaaaa atatgtaaat aattcttaga aaaacaggct
     1261 ctagacattg atcattttta gaaaaacatt tacatttgaa gaaaagcgtg gtaatctttt
     1321 ttggactata ttcctcaaaa gattaccttt tgcggtattt tatcgaaaaa aggagacgtt
     1381 acacatggta gagaaagttt atccaatgac tctagaaggt aaagaaaaat tagaaaaaga
     1441 attagaagaa ctaaaaacag tcaaacgcgg agaaatcatt gaacgaatca aaattgcccg
     1501 cggtttcgga gatctttccg aaaactctga atatgaatca gcgaaagatg aacaagcatt
     1561 tgttgaagga agaatcacaa caattgaaaa tatgattcgt tttgctcaaa tcatcgataa
     1621 cgacggtgta gatgcagatg aggtatcaat tggtaaaaca gttacattca tcgaactacc
     1681 agatggagaa gaagaagagt acacgattgt cggaagcgct gaagcagatc cgttttctgg
     1741 caaaatttcc aatgattctc ctattgcgca agcattgatt ggcaaacgta aaaatgatga
     1801 agtaactatt gctactccag gtggcgatat gttagttaaa atcgtcaaag tagaacaagc
     1861 gtagtctgac tgaaagatct agagagttca tcttactgat gatacactct agatcttttt
     1921 ctctttgtgt attttagatt tcaacaaacg atcttacatc taaagtccta tagccaacag
     1981 ttaaaaaaat aggtatgatg aaatgagaat gtgtcaggag ggagaaagag cgatgaatag
     2041 ttcttggcgt ttttttgtgg tagttgaagc actactgctg ttatttgctg tttggcaaat
     2101 cgtaaacaat actggacttt tgcttttagt gatattcggt attttcaata tatatctagc
     2161 tatgcgcaaa catcctcgta ctaaatttca aaattttcaa ttgatattag gaagtttggt
     2221 catttttttt agcttagtca acagtcctgc tttatggatg atggctgtat ttgccgtttt
     2281 atttattggt ctgaaaggtg tagagatttc agggatagat ttaaccaaaa atgctttttg
     2341 gcgaaagaaa cagatcatga tgatccaaac agagcaatta aaaacacata ataatgagcg
     2401 gaaaagacag caattattcg gtaaccaacg tattggaaac gacgtctatg aatgggacga
     2461 tatcaacatc gccgtcattt ctggtgatac tatcatagat ttgggcaata cgcttttgcc
     2521 aaaagatgat aatattgtga ttgtaagaaa agggataggc agaacacgga tcttagtacc
     2581 tttgggcgta gccatccgat tagagcatgc gacattagta ggaaatgtct tgtttgaaga
     2641 agagcaattt tctttgaaaa atgaacaaat caaaatctac agcagtgatt atgatgaaaa
     2701 ccctcgtaga ttgaagatta ttactaatac cttgattgga gatgtcgagg tgatacgaat
     2761 atgatgggaa aaatatccag agcgatgcta gctgtttatt cggggattgc ggctttcctt
     2821 attattctat tttcactttt cacttatttt tatgccagca atcaaagtca ttggtgggga
     2881 gaattactgc gtgcacgttt attatatgtt ccgcttatct tccatttgtt agccatttcc
     2941 ttaggtgtag gattaattgt ctttctatta ttatcactta ttcaaaagac aaaatacggg
     3001 aaaatcgaag aaaagctgcg tgcactttct tctggcaatt atgaatccaa attgttgctt
     3061 cttccgatcc caagtgcatc ggacgattta tacatcaaag atattgataa ggaaatcact
     3121 aagataaaag aaaaaatgat tgaagtatct agtgaattac aaattgtaac gagccgtccc
     3181 caatatgtag atggacaaac caaagaagaa attttagaat tagaaagaca tcgattagcc
     3241 cgtgagctgc atgattcagt ttcgcaacaa ttgtttgcag caatgatgat gatgtcggca
     3301 ttgacggagc aagcagaaaa aagcgagaca ccagaaatgt ttcgcaagca gttgaaaatg
     3361 gtagcagaga tcatcaacgc ttcccagtct gagatgcgtg cgctgcttct tcatctacgt
     3421 ccagtcaatt tagaagagaa aagtttaaag caaggtatcg agcagttatt gaaggaattg
     3481 caaaataaaa tccagatttc gctgaaatgg gatgtagaag atgtaaaact atctagttcc
     3541 attgaggatc acttgttccg aatcgttcaa gaattgttat caaatacatt aagacatgct
     3601 aaagccaatg aattagaggt atatttgcac aaaatagaca ataatctttt gttacgtatt
     3661 attgacgacg gaacagggtt taatatgaat gaaacgaaaa cgggaagtta tggattgaac
     3721 aatatcaaag aacgagtagc tggtattggt ggaacagtaa aaatcattag ttttaaaggt
     3781 caaggtacaa gtgtagaaat caaggtccct ttgatgaagg aggcatagat aaggatgata
     3841 aaagttttat tagtagatga ccatgaaatg gtgcgcttag gcgtctcttt ttatttatct
     3901 attcaagaag acattgaagt aattggagaa gctgaaaatg gacgacaagg ctatgagaag
     3961 gcgatgacac ttcgaccgga cgtcatttta atggacttag tcatggaaga aatggatggt
     4021 atcgaatcaa caaaagcaat cttaaaagat tggccagaag ctaaaatcat tatcgtaacc
     4081 agttttattg atgacgaaaa agtttatcct gccattgaag caggtgcagc agggtatttg
     4141 ttgaagacct ctaccgcaca tgaaattgct gatgctataa gggcaacgca acgtggcgag
     4201 cgagtattag aaccagaagt gacaacaaaa atgatggaaa aaatgagtcg gcgaaatgag
     4261 cctgtattac atgaagaact aacgaatcgg gaaaatgaaa ttttaatgct gatttctgaa
     4321 ggaaaaagta atcaggaaat agcggacgaa ttgtttatta cactgaaaac agtaaaaaca
     4381 catgtttcca atatattggc taagctagaa gtggaagacc gtacacaagc cgctatctat
     4441 gcattcaaac atggtttggt gaaataaaaa cgatgataaa gaggataatc aaaagctgag
     4501 aaaataacta ttatttttct tggctttttt acaataatga tgcttgcttt tgtttttgta
     4561 aaggctttgt tatactgaga aaagaagata ctaaaggagg aaaacggtga aacaaaattt
     4621 tgcaattgta ggtcttggac gatttggcgg aagtatctgc cgaacactga tcgaagctgg
     4681 tcaagaagta ctggctattg atagtaatga agatcgggtc aatgaatata tgaatatagc
     4741 cactcatgct gttgtggcaa atgcacagga tgaaatgaca cttcgatcat taggcataag
     4801 aaatttcgat catgtaattg tcgctattgg agaagatatt caagccagta tcttagttac
     4861 tctgatggta aaggaaatgg gcgtaccaaa tattttagca aaagcccaaa atgagtatca
     4921 tgccagagta cttgaaaaga tcggagctga tcgcgtcgtc catcccgagc gagacatggg
     4981 aataaggatt gcgcataatt tagtgtcaaa gaatatttta gattacctag agttgtcaga
     5041 taaatattct ttagcagaaa tccgagtctc caatcctaaa tttttcaaca aaacattagc
     5101 tgaattaaat ttcaggcaac gttttgactt aacagtagtc gccatacgaa gagtagatca
     5161 aacggttatc gcttctcctg cagcagatga atatgttcgt gaaaatgata atctactggt
     5221 tattggagag acaggcgatg tggatattct agacgataag atgaatcaat agaaagaaaa
     5281 gagaaagtag gttgttggga tgaaattaac ggtaacgcca aaagcacaaa agtggtttga
     5341 ctcagaaatt gatctgccag aaacttacgg aatacgtttt tttggaaaag tctacggcaa
     5401 aacagaggtt catgatggtt tttcgattgg aatgtcagtt gaacagccag aacatcctgt
     5461 aaagaaagtg gaaattgatg ggacactttt ttttatcgag gaagcagatg aatggttctt
     5521 taaaggatat gatttacttg ttgattatga tcccaacctg gaggagccaa cctatcactt
     5581 taaaaaacaa taaaaaaaga gagggtgaca caatttttgt caccttctct tttttctttt
     5641 gaaaatattg ttttcagcta ttaatatcct gcagacaatt ttacttgatt ggtttgaagc
     5701 tcatgggtgg caaagtagct tttcagattg gttaaaaaaa tagccattaa tttcttttga
     5761 aaatcaggtg tcaaacctgc aatgtgggaa gtaatcagta cattgtccat tttccaaaga
     5821 ggatgatctt gtggaagagg ctcttcctca aaaacgtcta atgctgcaaa ggaaagttgc
     5881 tggttattta aagcctgcac cagatcttgt gtcttaacag aagcaccacg gccaacattg
     5941 atgaatacag attttggatc aaagtgttca aacatatgct gatcaaagag cccagttgtt
     6001 tcctccgtta gaggtaagat attgactacg aaatctactt caggaagaac ttcctttagt
     6061 tgatttaaag aaaaagtctt ttccattcct tctgctggat gaccggatgt attgattcca
     6121 tagcagttca tgtctaatgc tttgcatttt tttgcaatca tttttccaat atggccagtt
     6181 ccaatgatta gtattttttt cccttggata gattggtaat ggaccgatga acctaaccat
     6241 ttcatgtcac tctgtgcctt ttgtgcttga aaaagaccac gagaataccc aaggatgatt
     6301 cctaagacat gctccgtaat cgattcactg tggatgccac taccatttga aagtaaaata
     6361 tgtttctttg aaaaagtctc aaggggtagc tgatcaacac cagctgagat agattggacc
     6421 catttcaagg tagaagaggc taatagtttt tcttggtagt ctgagttcca gcccaaactg
     6481 atctctacat tttgtagatc ctctggttgt aattctgttt taaagatata accaggcgct
     6541 atagtatgga tttcttcgat aaattcatca cgaaatggac gatctgcata aatgattctt
     6601 tctgacatag ttaatttccc gcctttcttt actctaagtg tagcgcatac aagaagaact
     6661 ttgaagcgcc ttgcttgttt tttgagaaag aaatattttt caaataaaaa aggggctgtg
     6721 acaaaaaaat gtcacaacct ctaaaagggt ctattgaagt agaaataacg agttgtcttt
     6781 atttcttaat tgatcgatat ttttgcccat cctttgacag cttgagcaaa aataaagatt
     6841 cctgcaaaag caatgaaggc gctagtgtca aaagaaggaa gaaagaccat caagattgtt
     6901 gcaattatag taggcaatga aattgaatag atcaatgttt ttaaacaatc aagaaaagta
     6961 gcttttgtta gtcgaaagcg tgcatagaga aaagcagcaa tgttggtaaa tagcaaagtg
     7021 atcagcagat tcagaaaact aggataaata gaaaatataa atgtcaggac ttttatccaa
     7081 agtggtatag atgcttcact taaaaaagaa cggagctgct tacctgttaa gttttttaaa
     7141 gattcattgg tgtagggaag ttctaattgg ttattgttca acaaagcaga agtcgtgcct
     7201 gtattaggaa gagctatgat aagtttgttt tttaacatcc cgacactaac aaaattcccc
     7261 atcaaatcag tagagatgtc ctgttctgtt cgttttccct ctggatcaaa tgtgaagata
     7321 atggagtttg tttggtaaat gaatccttct ttttcttcgg tttgaagctg cccgttttgg
     7381 attgtaaaat caggaatgcg agtagcaatc ttttgcccat ccaattgaat gttgtttaag
     7441 acttgaaaaa cttgaaaaga gattggcaaa gccataatta tactcaaaac taaaagatag
     7501 acaatggctt tccaaaatgg gacatttctc gcctccttta ggtctttgaa tcgtataaag
     7561 gaactgataa ttaattgttt tgttgtcata agtactcctc atctagtagt gtcatcactt
     7621 gcaattctag cgaagtatca agtgaaatac aagtgcttta aggaaacagc tgaatgattg
     7681 gtgaaatatc aaaaaaatgt tattcttgtc taaagactat ccaatataaa taaaatggag
     7741 gaaggaatag tgcctacaca gccacagaga tcatcaaaaa aactatttgg agtagtggtt
     7801 ttgaatgcgg tgatcacttt atcagaattt attggaggga ttatgtcagg gagtttgtct
     7861 cttgtttcag atgctttcca taaccttagt gatacattgg caattatttt ttcttacggg
     7921 gcacaaaaaa tggcccgaaa agaagcaaat aaaaaacgta cgtatggcta ccagcgttta
     7981 gaaatattga gtgctttcat caactcattc atattgatca tattatcctt gtttttaacc
     8041 gttgaagcat tcaagcggtt cacttcacca gaaaagataa atagtcattt gatgctaaca
     8101 gtagcagtta ttggattgct cgcgaatttg ttctcagtat tattattacg acaagaagca
     8161 gatgaaagtt tgaatataaa atctagttat ttgcatttgt taagtgatac actgtcttcg
     8221 gtaagtgtga ttatcggagc aattttgatc cgttttttcg atatctactg gatcgatcca
     8281 gttattacac tgatcatttc gatatatata ttgatagaag cgataatggt gatcaaaaaa
     8341 gctgccgcta ttttgataca aagtgcacct gctatcgact atgaaaaaat ggaacaagaa
     8401 attgaagcaa tcgatggggt aaaggatgtt catcatgtcc atatttggca gtatagcgaa
     8461 aagataatta ttttcgacgg acatattgat tttgaagatc agctactaag cgagattgaa
     8521 aagatatatc ctcgtataat ttctttatta aaattaaaat atggtattac acatgtgacg
     8581 atccaagcag agacaaaggc aaaggatcaa aaaaaattaa tttttttaaa taaagatgaa
     8641 agttattgaa atatagagta tgataaataa gaatttttaa taaggtaggt tggctcatga
     8701 agcagtatcg acagttaaaa ggatatttag aagcaaaagg atattgggag atactagtct
     8761 atctcttttt tggtggtttg gctacgattg taaattttgt tagttttgct ctcgcacgcc
     8821 aatattttga tttaagtatg gccttatcca attctatttc gtggttttgt tctgtattgt
     8881 ttgcttttgt aacgaacaaa ctttgggtat tccattcaaa atcaccgaat tttacacatg
     8941 cacttattga atgcggtaag ttcttcttct accgtatcct ttcttacgga ttggacatgg
     9001 gggcaatggt attgttgatc aatgttatga acagcaatga gtatgtggcg aaaatcatca
     9061 ctcaaattat tgttatcctt gcgaactata ttttcagcaa gttgtttatt tttaaagaaa
     9121 ctgaggtttt tgaagaagaa gtaggtcaaa gtgaaaaaga cgactaggca taacaattga
     9181 agaatttcct tctctttgtt actctttgta atgtagacat tattaagaaa acattgagga
     9241 ggaatttttt tatgacttac acattaccag atctacctta tgcatatgac gcattggaac
     9301 cttatattga cgaagaaacg atgcatctgc atcatgataa gcatcacaat acttatgtga
     9361 cgaatttaaa ttcagcaatt gagaaatacc cagaattagg cgaaaaaaca atagaagaat
     9421 tattatctga tatggacgct attccaacag atatcaagac agctgtacgt aacaatggtg
     9481 gcggacatgc taaccattca tttttctggg aaatcatggc accaaatgct ggtggcgaac
     9541 ctacaggaga aataaaagaa gcgattaatg aagcttttgg tgatttttct tcttttaaag
     9601 aagaattcaa aaaagcagcc gctggacgat ttggttctgg atgggcttgg cttgtaatgg
     9661 aaaatggaaa attagctatt acctctactg caaatcaaga ttctccattg atggaaggaa
     9721 agacaccaat tctaggtttg gacgtttggg aacatgctta ttatttgaaa tataaaaatg
     9781 tccgaccaga ttatattgca gcattctgga atgttattaa ttgggatgaa gtaaacaaac
     9841 gtttcgaagc agcaaaataa ccatttcctc cgtcatatca gtcttaaaaa taatttggaa
     9901 aataaaagag cgaaagcttg ctagcgtttt ctaaattggg tataatgttc ttgcaaggtt
     9961 acttgcaaga acttttttgg aggatataca tttatggaac acggaacggt taaatggttc
    10021 aacaatgaaa aaggatttgg gtttattaca gtagacggcg gagatgacgt atttgttcat
    10081 ttctcagcaa ttcaaggaga cggcttcaag agcctagaag aaggtcaaga agtagaattc
    10141 acgattgttg aaggtgcacg cggacctcaa gctgctgaag tgactaaatt ataataattt
    10201 gttgaataaa gacacgactt cttagagagt cgtgtctttt tgtctattta tgaagttttt
    10261 agattttttg gctaagtaaa tgcatttgtt cacttgacat gtcctaacta attatcaagc
    10321 aaggaacatt ttcttcgtta gtaatggatt ttaaagaaat ttcagttttt ggatgttgaa
    10381 taaaagacca tttgtgaaac cactagtata catactcatt accatgtcaa ttctattact
    10441 ttaaaagaaa atcgttttac tttgaagagg agcatatcta tccattaaaa gatgatggta
    10501 atgcaatcac tagttcgagt cattttaaga gccatgcagt gagctggaaa taaaacgaat
    10561 agaagatggg tttaacaaaa ggaccagtca taggaagaaa ttgtcgtgaa atcgaatgat
    10621 aatctgaacg gtaggcaaag actgttaaaa agatgggaaa ggtatgtgcg atcattttac
    10681 tgcacgatta tttggaaaat accttgttgt tcgctttttc tggaaaaaat acataaaaaa
    10741 agataaaacg attgcgaact gcgtgtattc ggtctttcga tctttttcac ggtatggtat
    10801 gatagttaag aattttgaga aattttaaaa aataataatc gttacacaaa aatgtttttt
    10861 aaagttttct tatgattaaa aagtgaattt gctgattgaa cagttatatg agacaatatt
    10921 ttagaaaatg tgaaacagag gtgtattatg aagcgatggc aaaaagttct attaaccttg
    10981 ttaggggtat tcgttttgct gattgccgga ttatcagcat acggaatcag atttatggga
    11041 gaagcgagcc aaacagtaaa caaaatttct aaaaactcta atcgaatatc ctccaaacgt
    11101 tctgagagag taagcattga tgacaaagaa cctttttcgg ttttgctatt aggtttagat
    11161 accggtggat taggaagaac ggaacaagga cgttctgata cgatgatggt agtaacagtt
    11221 aatccgaaac aaaaaaaatc aacgatcatc agtttggacc gagatattta tacgaatatc
    11281 gtaggttatg gaacggtaga taaattaaat catgcctacg catttggtgg tgtcgaaatg
    11341 gccatggatt caattgagca actgctcgat attcctattg atcattatgt aacgatcaat
    11401 ttggatggca tggaagattt gatcaatgcg gtcggtggca tcaaagtgaa taataaaatt
    11461 gactttactt tggatggtgt ccatgtacca gtaggtgaac aaacattgaa tggagaaaaa
    11521 ggtttagcct attctcgaat gcgacacgaa gatccagaag gcgatatcgg tcgacaacgt
    11581 cgtcagcggg aagtagtgac gaaaatcgtc aataaagttt taagtttaga tggcgtaagc
    11641 aattatagaa aaattttaaa agcagttgag aacaatgtga cgacggactt agattgggat
    11701 gacatgcttg atgtagcgac gaactacacc ccggcgtttg aaacaatcaa acaagaccag
    11761 ttgcaaggcg aaggacaaat gatcaatgat atctactatc aaattttagg aaaaaatgat
    11821 cttctttcga tccaaaatga actgaagaaa caattaaata taaaaacaag cgatacatta
    11881 cctaatttga aaaatgagaa tgcttcaatg atgttctttg atgattcgga agatggcgat
    11941 tcgacaaacg gtgatggatc gaatagtgaa gggtacaccg attattcttc ctattcagaa
    12001 aacacatatg atcagtacca agatccgaat acctattatc agcaaacaga agatacaagt
    12061 cagtactata caccaagtca agatactcaa agttatgatc cttacgctgg acaggaacaa
    12121 aatggttatg gttatgacca gaataatgag tatggaacaa caagtgggta ctagaaaaaa
    12181 atagtttggt taataaagag tgccacctaa attagacaaa aaa
//
LOCUS       DAYYGO010000083        12207 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000083 DAYYGO010000000
VERSION     DAYYGO010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.085"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            350..580
                     /locus_tag="ACHA5T_002000"
     CDS             350..580
                     /locus_tag="ACHA5T_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887564.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMGREAE
                     PCEIDACKLVYCGHGFYDEPNIQNNINKNLRD"
     gene            739..1572
                     /gene="terS"
                     /locus_tag="ACHA5T_002001"
     CDS             739..1572
                     /gene="terS"
                     /locus_tag="ACHA5T_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFM8887565.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELISNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            1565..2980
                     /locus_tag="ACHA5T_002002"
     CDS             1565..2980
                     /locus_tag="ACHA5T_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFM8887566.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPESMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTKVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            2992..4521
                     /locus_tag="ACHA5T_002003"
     CDS             2992..4521
                     /locus_tag="ACHA5T_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8887567.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKAPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            4439..5491
                     /locus_tag="ACHA5T_002004"
     CDS             4439..5491
                     /locus_tag="ACHA5T_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFM8887568.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            5493..5810
                     /locus_tag="ACHA5T_002005"
     CDS             5493..5810
                     /locus_tag="ACHA5T_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887569.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            5937..6560
                     /locus_tag="ACHA5T_002006"
     CDS             5937..6560
                     /locus_tag="ACHA5T_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HFM8887570.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            6575..7465
                     /locus_tag="ACHA5T_002007"
     CDS             6575..7465
                     /locus_tag="ACHA5T_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 family protein"
                     /protein_id="HFM8887571.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            7488..7703
                     /locus_tag="ACHA5T_002008"
     CDS             7488..7703
                     /locus_tag="ACHA5T_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887572.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            7715..8056
                     /locus_tag="ACHA5T_002009"
     CDS             7715..8056
                     /locus_tag="ACHA5T_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887573.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            8056..8427
                     /locus_tag="ACHA5T_002010"
     CDS             8056..8427
                     /locus_tag="ACHA5T_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887574.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            8420..8815
                     /locus_tag="ACHA5T_002011"
     CDS             8420..8815
                     /locus_tag="ACHA5T_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFM8887575.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGEVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            8817..9194
                     /locus_tag="ACHA5T_002012"
     CDS             8817..9194
                     /locus_tag="ACHA5T_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6838 family protein"
                     /protein_id="HFM8887576.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNNPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            9195..9803
                     /locus_tag="ACHA5T_002013"
     CDS             9195..9803
                     /locus_tag="ACHA5T_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFM8887577.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            9803..10144
                     /locus_tag="ACHA5T_002014"
     CDS             9803..10144
                     /locus_tag="ACHA5T_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887578.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            10386..>12207
                     /locus_tag="ACHA5T_002015"
     CDS             10386..>12207
                     /locus_tag="ACHA5T_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFM8887579.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANPFVLIIGAVVGAI
                     AVFGYFMATNEELRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNGIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIK"
BASE COUNT         4427 a         1694 c         2517 g         3569 t
ORIGIN      
        1 gtagcagcca agaataattt tatagtgtca ctgtggcgga aagggtagac gctaagcatg
       61 tgtgctaggt caatgcttcg gcaaccatgc aaggttcgat tccttgccag tgactttggc
      121 aaccgaggca tcggcggttt aaaaatatag gggtgcgcaa tttcgtacgc gttttgtgca
      181 tcgtgtaggt tgctattaca taactggtta ggttagattg agatttggga ttcggtacaa
      241 atgaatcgtc aaatgtctca agcacaggat cggaaacgtc cctgcctgtg cattacatat
      301 tagatcactc tttgagtggt ctttttcttt acataaagga ggctacataa tgagaaacta
      361 ctggtatgta tcgctaacta atgaatatcc tcgaaccatt gatgattgtt cagtgcgtgt
      421 tgtgcgttct gtacaaatca aagggaagta ctctattgtc gaaatgggga gagaagcaga
      481 accatgtgag attgatgcgt gcaagctcgt ttattgcggt catggtttct atgatgaacc
      541 aaacattcaa aataatatta acaagaattt gagggattag aatgcaagaa atggcttaaa
      601 aatctccaat caggacaaat caagttagac aaagtttccg atttaaagat attgattgaa
      661 gctgatttaa tgctaaaaga tattgaaaat tagaaaacaa aactcaacct aaacagaatg
      721 tgaggtggtg tgtattgaat ggcaagacaa cgtgatccaa gacgtgatga agccaaaaga
      781 atttggttag aatccaacgg agaaaagcag ttaaaggaaa ttgcatctga attaaatgtt
      841 tcagattctc aggttagaaa atggaaatcg caagacaaat ggagcgctga attgattagt
      901 aacgttacca atggcaaaag taacgttact aatcaaggtg gcgctcctat tggtaatcaa
      961 aatgctaaag gtaacaaagg aaatagccga gcttctccgc cagtgggtaa taaaaacgct
     1021 ttgaaaacag gtgagtatga aacaatattt tttgacacgt taaccgataa tgagaaggac
     1081 atctattcta gtttgaatga tgatccttct tttgctttgt ccgaagaaat acgacttctt
     1141 aaaataagac aacttcgaat gatgaaaaga atccaacaag ctgaagctgg actaaatgat
     1201 gaagaagtcg aacgattgca gcagctaaga aagattaaaa ctccgattga aaaaaatggt
     1261 aaaaagctag aaatcaagcg tgaggttatg caagatgtgc agattagcag aaaaaaacat
     1321 cgcaaaattg atgatattct ttcaattgaa gattcattga ctcggattag caaccagtta
     1381 gctaaagcca tcaagcaaat gaatgaactt tatatgaatg aatacagaac tgatttaatt
     1441 aaagctcaga ctgataagat ccaagctgag acaaatgaaa ttggcggaaa taattcaggt
     1501 gaagaaatag aagaatggaa acaggcagtt ttaaatgccg caaacaaacg ggcggtgaaa
     1561 gaaaatgaat aatgaattta ttccttttgc tgatattggt tctgccattg attattacta
     1621 tgataaacca gtagcttttt gccaagatat tttgcatttg aatcctgatg aatggcaaga
     1681 aaatgtttta aatgatttag ctgaattttc aaaggtttct gttcgttctg gtcaaggagt
     1741 tggaaaaaca gcattagaag caggagcaat actttggttc ttaacgtgtc gaccctacgc
     1801 taaagtaata gcaacagctc cgacaatgaa gcaactttac gatgtacttt gggcagaggt
     1861 agctaaatgg ttaaatgata gcttgatcaa aaacttactg aagtggacaa agaccaaaat
     1921 ttatatggtt ggtgattcag agcgttggtt tgctacggct agaacagcga ctaaaccaga
     1981 aagtatgcaa ggttttcacg aggaccatat gttgattgtg gtagatgaag cttctggtgt
     2041 gtctgatcca attatggaag ctattcttgg tacgctatca ggttttgata ataagctgtt
     2101 gatgtgtgga aaccccaata atattgaagg tgtcttttat gattcccata attcagaccg
     2161 tgataaatac agagttcata aagtatcaag ctatgatagt aaacgtacaa acaaagacaa
     2221 tatagaaatg attcttaaaa aatatggaaa agaaagtgat gttgctcgtg tccgtatttt
     2281 tggagaattt ccgaaaggtg cgttggattc atttatcagt cttgaaacag ttgaattggc
     2341 tacagaaaaa caaattagtg attctttagt caataaaaca aaggttgctc atattggtgt
     2401 cgacgtagct cgatatggtg atgattctac gattctcttt cctagaattg ctaccagggc
     2461 attggagtat gagaagtatt caaaacgtag caccatggaa acaacaggat atgtcatcaa
     2521 catggccaag aatctaatga gtcaatatcc gagtattgat aaagtgatga ttaaagtcga
     2581 tgacactggt gtcggaggtg gtgtaaccga ccgcctagaa gaacttatag aagacaaaca
     2641 ttatcctttt gaggtgtttg gagtgaataa cggttcaaca tcagaagacg atttttacga
     2701 taatttaggt actcaactat ggggaaacat caaggaaatg ttagaagaaa atatgacagc
     2761 aaatcttaac ggagaacagc ctgttattga attgccttct gatagttcgt taatcaaaga
     2821 attaagtact cgcaaattca aaatgacaag tagaagtcgt atacgtttag aaagtaaaga
     2881 tgatatgaaa aagcgaaata ttggtagtcc cgatattgct gacgcactgg ctttagcgtt
     2941 ttatgagcca ccaagtcact atcaatttat tcaattttag gaggtgagct tttgattagt
     3001 acagtattat ctttagagag atataagaag ctacgtgtta aatatgcaac acaaattgaa
     3061 gatggaatgt ttgatccgaa tggttttata gaagatatga aaccattttt tgctgatcgc
     3121 gaaagaaaat atctagctta tactagcgaa aagaatgaaa tagataatag accaaagcct
     3181 aacactgata ttgtaaaagt taataataaa cttcatgctg gtatgtattc aattgtcgta
     3241 gaccaagcag tcaatcattt tactggtata cctatcaaat gggattacga tgtatcggaa
     3301 caaaaaagaa cactcattca aagattaaaa gataaattct taaaaaatga tatagaactt
     3361 ccgacagttc cagaagcttt taacaaatta acaagcaatc ttgattccat gagattcgca
     3421 atgcttgatt ctgaaactgc aacttttcaa ggagcttgtg gagtagcttt ccgattgcta
     3481 gagcctgtgg aagaagatga tggttggaag ttaagagcaa gtaatattga accttggaga
     3541 gcagaaagat atggaaatgc cggaatctat atcaaagaaa agtatgactc ttaccagaaa
     3601 aaattttttc aagaaatgaa agttatgaca agaaataaaa ttctgacata tgcttgctat
     3661 ggtgatttga attttgttac aagcggaacg ttcaaaaaaa ttgatgagac cgataaccct
     3721 ttggggacga ttaccttggc agaatttaaa aataatacga atcgttattg tgattttgaa
     3781 gtagctgagg aaattggaga tgcaattgat cgtgctttgt ctgatcaaca aaacgaaatc
     3841 gaacaattta agcttgcgta catgcttgtt actggcacaa caatgagcaa aggcactgct
     3901 aaagaaatga tgaatcagct agggattatc aacttaaaag cccctactgc aaaagctgaa
     3961 tatgtcacaa aaaatttagc aaaagatttc aatgaatatc atatggatct attgaaaaaa
     4021 cagttttaca ccatttgtaa agcaatcgat tttaacgatg aggtgtttaa atcaaacagt
     4081 tctggagaag ctcgcaagtg gcaaatcatt agcctagaag cgaaaacaaa tactaaagag
     4141 caatatttta gagaaggatt gaaagaatgc gcagaaacaa ttgctgcttt tcttaagttt
     4201 catgacaaag tagaaattga gcctgaaaaa attattttta ctttctctag atctttgcca
     4261 actgatttaa gttatttagc tgaagcttta cctaaacttg caccatacgt atccaaacga
     4321 actattcaga gccaaattcc atttgtaaca gatgttgatt atgaaaatga aatgatggaa
     4381 ttggaaagtg gaagtgctta tccagatagt gaatacaatt ttggcggagg tggcaatagt
     4441 gacgatagaa acgaagtatt ggaccaaacg tcgagaactg gaggatcaag caaggctcaa
     4501 acaagaaaat cagacactta aaaaattaac tagtgtattt cctgaagcac tgaaagagat
     4561 acaagcaaaa ctattatcac aagctgactt acacaatatc acttatccag aaatgatgga
     4621 gttttacagt acaagtaatc agaaaaaata tcgtgaatat gtggaaaaaa attataagtc
     4681 attaaaaatg tatgatgcaa aatacaaaga gtttatcgat gaattttttc caccatttga
     4741 ctatgcaaaa gtcaatcgct tattacaaat acgatcagat gtatttaaaa ttcttgcaga
     4801 atatgcgatg gatgcagatg tgaatcaata tttttctgat cgcttagagg aaattcttca
     4861 aagaacatat tcttctaatg ctaatgtttt tgttcaactt ttaaacgttg atataccaaa
     4921 ttatttacca gaaaatgaaa ttaaacatta tttgaactat ccatggtcag gaaagacatt
     4981 ttcaagaaga ctttggggaa atatttcatc gcttgaacag aaactctcta atgctattgt
     5041 aaaaagtgtt gctagtggag aaggcgttat acacgcatta aacaccatga gattagactc
     5101 agaaatttgt gacatgttta agttagaaga atcaaagtat aacaaagcga tagaaaatct
     5161 cgttcgaacg gagtatgcaa aatttgcaca agatggtatt gaaaaatcat atttagaaac
     5221 aggtattgag gaatacaacg tattgactgc aaaagatgag agagtttgcc ggatttgtgg
     5281 aggaaaggca agtaagaatc cctataaact gaaagatgct gtcataggtg aaaatcgagc
     5341 acctttccat agtcgttgta gatgtacgga tgttcctaat ttaccaaaat taggaaagga
     5401 tattgatgaa gaatatgacc gtttatttgg cgatttatta gatgagtttg cacatgattc
     5461 ttttggaatt aatttgaaac ggaggaagta gaatgaaaga tttttttgaa gcagtactaa
     5521 caattaatgt aaatgctgat attgcagaag cctacaaaac agctattgag tctgagaacc
     5581 atcctaatgg cttgagagac cattggaatg gcaattatgc ctacgtggtt attggcgatc
     5641 aaactgttaa ttatcaagat aatactccag ttgataagaa taccgttaat ttaacgattc
     5701 aattattatc tcattcatta ccaaatttaa aagaaacagt tgattggtat gaaaagatgg
     5761 gatgtattgt tgttagaact gactacaaag aaggaaagtc tagtaattag gcttttttat
     5821 tttgtccgaa atgacgttaa actagcgcaa tactgggctt ggttgaatgg tggggcgcaa
     5881 ctattaaaac tcaaagcaat gcggggcgtg aaaacgaatc gtggggcgaa aggagaatga
     5941 gcatgaaaca caaatcatta atgccaatga atttgcaata ctttgccgaa ggtgacgatc
     6001 aaaagttttc ttttgatgac ttcaaatctt ttgtggaatc caatgaagaa gctcaaaaat
     6061 ttattcagtc acaatctcaa agtgttgccg acaaacaatt ggaagcttgg aaacaaaata
     6121 acttagataa aattaaacag gataccatca aggaatatga ggaatctaag aaaaacaagt
     6181 cacctgagca aattcaactg gaaaaactac aagctgaatt tgaagcagaa aaagcgttgc
     6241 gtgtgacaag tgataataaa gcatttgttg cagaacaaat tgctggatta gaactagatg
     6301 gagagttaaa agagtctatt tctcaattta tgctaaataa tcttgttagt tcggatacag
     6361 atttcactaa gaatgctgtt gaaggtttta caagtgtttt gaatgcaatt aatgagaaac
     6421 atgcagatgc attaaaagaa ctacaaatga agtctgcatt tggtggaact caacaatcga
     6481 ataaccaggt tcagcagaac aatgaaacat taacaaatcc agaagaacaa ttaggacaaa
     6541 ttcttcaaca atttaactag gagagtgaaa aattatgaaa aaaacatctt taaataattt
     6601 agagtatttg gatatttcac cagcgattaa tgctatgcaa gtaccaaata cacctttttt
     6661 aagctattta cttggtgctg gaaaaacaga gccagcaaac tcgacagaaa ttaaatggcg
     6721 tgaatatgat atcaacaacg atgattcttc tgaaaaactt gagggcggag aatatccaga
     6781 tgctgaatca ggtcgaactt ggtttaacaa ctatactgaa atttttagaa aatcaacctc
     6841 tgtatctggt acattagatg ctattaatgt gaatggagtc ggaaatgaat taactaatca
     6901 agtagcacta cgtggtatgg aaatgaaaat tgatttgaac cgaaaattga ttactggtgt
     6961 aaaagctgat gaaaatggtt ctaaaggtcg tcgaatgaat gggattttga acttgatcaa
     7021 ttcagcaaat aaggcagaaa cagccactgc gggtgcagta acaagaaaag atatcgatac
     7081 tttatttaaa ttgatgtatg aaaaaggtta tatgggagaa aaactatgct tgatttctcc
     7141 agatatgcag gagttaatga ctgatgagtt agatggaaaa tcaacaaaaa ttgttcagtt
     7201 cggggaaaga gtaacttttg gattgcaaat tggaaatatc gtgtctaatt acggtacagg
     7261 tattgctcta ctagaaccat cattgccaaa aggaacaatt gccgcaatcg atactaatta
     7321 tgtgaaacta cgtccattac gtgaatggag agcagaagaa cttgcaaaaa caacggattc
     7381 tagacgtatc ggtcttgtag gtgaatactc tcttgaatac aatgcttcaa actctggggc
     7441 aattttaaat ttaaagtctg aataaaaggg agttagtact cccttttttg ataggaggaa
     7501 ttacagtggt aaaaaaagat gaaactaaaa aagatgaagt cgtgaaatat agagtaggta
     7561 aaactaaaaa ttttgttggg tttgttcatc ctaaaactcg tagatttatc acagcagatt
     7621 caaataacga atttatcatt tctatagatg ataaagaagc aattgcaatt ttggaagatg
     7681 caattgatgt taatgaaatt taggaagtga tctgatggat gaatcgctaa aaacggaaat
     7741 cattgagtct acaaaagaag attttccaga tttgagtgaa gaacgcataa ctaatttatt
     7801 agaaataatt ttgctagaaa ttgaatcata caacacttgt aaaaatgata tttcatggga
     7861 aaagttaaaa agcgtgatta gtgaagtgtt gtataaaata ataaaaaatg aatcagaaaa
     7921 aacagtatct tcagttagac gtggcgatac aacgattagt tatgcttcaa caacaaatga
     7981 tgttagtgag ttacttctag gctatggtga tttgatacga agagttattg gttgtggagg
     8041 attggagttt ttttaatgaa tgaagcagat attttagaaa ctacttatga agatagttgt
     8101 attattgaaa gactgacgga cattgaagat tctaatacaa atattactat tcaagattac
     8161 aaaaaagtat atgataatcc tatttcttgc gctctttcac aaggtcaaat cgatggacta
     8221 gcagtcatag aagatggaga gatggtaaat gtttcaactg acacatataa attatttgtt
     8281 catcctaaga ttaaactaaa aaaaggagat cgaataacaa taactcaaaa ggccagtggc
     8341 ttaatttttt ctctatttgc tactaagcct ttttactatc ctagtcattg tgaagtgaat
     8401 ttgataggaa gtgagaaaaa tgggtaatct caaatatgaa tcaaatgcag aaaagatcat
     8461 tgagaatttt aaaaatatga ctgtaattgc tcaaaaagaa ggaatatcct tcgttaacga
     8521 ttcaatgaat aaagtcgtta gtttagctaa accattaact cctgtaaaat caggtaattt
     8581 aaggcgagga tatcgtgtag taaaagctag aaaactatca agcggtcgaa ttgttggaga
     8641 agttataaac aatgaacatt attttaaata tgtagaagaa ggtcatagga ctaaaaatgg
     8701 tggctttgtc aaagggaaat tcatgttaac tcgtgcaacg aatcttgcaa atatgtctta
     8761 tattcctcga agatttaaac aaatggcaat aaaaatcgtt aagaaaggaa agtaacatga
     8821 aagatgaaat cattgctgca atcagtagca aattaaaaga aatctatcct gatgggacaa
     8881 tatatcttga ttcagttatg cagtcaacta aagattttta tttcgtttta tcagtaatgg
     8941 aatctggaac tgaaaatgta ggaattgata ttcaaaatgt ttctttctta attgatattg
     9001 cattgattga taataaccct aatagaaatt taattaatga attagtctca cgttgtggga
     9061 ctttttttaa tacgattaca atcgatgagc aaatattatt tccaaaagcc tatttacctg
     9121 atgaagcaga tggtgttcaa catattcgtt ttacattaga atttccacaa tatattgaat
     9181 ggagtgaaag ataaatggga gaaaaaagaa gtaaaaccgg aattatttct gtcgaaaaac
     9241 ctacttggtt tcctttggaa gatgagacag ggaattttcc ggtatatggt actgcgttgc
     9301 caatgggaac agcagtaagt atcaaaccaa cagctaatta tgaaacaaca caagattatg
     9361 gggattcagt ggttcaagat caatttacag cttttggtgg tgcagaagtt gaactggaag
     9421 caaatggata tactcataaa gttttatcta caattactgg agggaaaatt gttaaaggtg
     9481 gtgccttgcg ttctggagaa gatattgcgc aagatggtgc gtttgcatat agacgtaaaa
     9541 aatcgaatgg gaaataccgt tatacggtct tttataaagg acagtttgct ctagattcag
     9601 atgaaacatc aacgattgaa ggaagtaaag taagttttac tcatccaact tggaagggtt
     9661 catttgtaga tgtaccagga cttggatata tgtattcagt tgatgaagat gatgaaggcg
     9721 tggataaagc aatgatcgga aattggttta caaaagtagc aattccaatt gaagaagcag
     9781 aactttcagg aggtacagaa taatgtcaaa atatcaaaca acgattaaat taacgaaaaa
     9841 agacgaagaa ggtaaatatg agcaagtaca atttaaatct gctgaatttt taccaggaac
     9901 agttgtagaa gatgctgcag gagttatgga agaaatgcaa acagcgactg ataagcaatc
     9961 agttaaaaaa gctttaagtc gtgcttattc atttattgca gatactttat ttgagggaca
    10021 atttactggt gaagattatt gcaaaggaat tgatgctcgt gagattgcct cattgacagg
    10081 aaaattattg aagtctgtta ctgcaggttt tgatgaaact tatacagaga cgaaaaaaaa
    10141 gtaagtgagg cgctcaagtc accttcattt aagtattcga ttacttaccg agaattagat
    10201 ataaagacgc aattactcga agcaggttgg acgttaccag aaattgaacg tactgacttg
    10261 gatgagttaa tgcgtctttt tgcttttaga gatgcggtaa aagagcatga agatgttgag
    10321 tactatgata atttcactca attttaggag gtgatacttt gaataacgat gacctaattc
    10381 tgaagatgat actagatgaa tctggtttta ctgccggtat gaataatgct gtcaaaaagt
    10441 tgaactcttt tgatgaaaca atcgaaagaa aaagtagaaa tagtggcagt tcattaggta
    10501 gtatttggaa aatatttgct ggaagttttc tagctagcgg agtaactaga atcgtaggag
    10561 ctggctttga tctaatcaag ggttccataa gtggagcggt tgatcgagta gatacgatga
    10621 ataacgctct acgaaatttc caaaatatgg gatttagtaa ttcagaaatt atgaagaata
    10681 tcggaaagaa tgggctttta tctcaaggta ttcaaggact tcctactgct ttgaatgatg
    10741 cgataagtca tgttcaactt cttgcttctt ctacaggtga tttaactcgt tcaactcaaa
    10801 tatttaaagc tttaaatgat ggaattcttg gtttcggtgg ttcaactgat caagttaatg
    10861 aagctgttat tcaattatct caaagtttct caaacggaaa agtagacgca caaacttgga
    10921 actcaatgat caacgctcaa cttggaccta ctctttctgc tattgctaaa aagatgggaa
    10981 ttacaatggg agatctgaaa gaaggtttat ctcaaggtaa gatttctgtt gaagaattcc
    11041 aaaatcaatt aatagaaatg gataccaaag gtggcggagg acttaaatca ttaagtcaaa
    11101 tcgctaaaga ttctactaag ggaattaaaa cctctataca aaatgcaaaa acagctgtta
    11161 cacgtggtgt tggtgaggta atagaaggat taaataaagc attagtagat tcagatttag
    11221 gtggatttaa gggaattatt gataaagtgg gcagttcaat ggaatcgttc ttaaaagtaa
    11281 ttgctgcaaa tattcctaca gcagtatcat ttttaagtaa tttatttaac gaaattcaaa
    11341 aatttggttc tgctttgaaa ttcatgatgc cctttcttgt tcctgcagct accgcttttg
    11401 gagcgttcat gtttcaactt aaaggtatac cagcaataat aaaaagcttc aataatttta
    11461 agaatgccat aattggcgtt ggaaattcat taaagattat gggggcaata gctgctgcta
    11521 atccatttgt tttaattatt ggtgcagtcg ttggtgcaat agctgttttc ggttacttta
    11581 tggcaaccaa tgaagagctt agaaacaagg ttatatctat ttggaatgat gtgaaagatt
    11641 ccgtaattgg tgtattaaaa aatataaagg actggggaat tgatacttgg aattctgcta
    11701 aagaaatggc atcaaatgca gttgagggtg tcaaagatgc ttggtcagga acaaaggaat
    11761 ggttttctaa cacctggaaa ggtattaagg atggggccac aggtttattt gataaaacag
    11821 tagagacttc aagaaatgca gtcgatagtg taaaaaatgc atggtctaat atgaagaaat
    11881 ggttttctga tacttggcaa agtataaaag attcagccag agaaaaatgg aatggaatca
    11941 aaggttctat tatgtcagtt gctggtccat tgattacagg tataaagaat gcatttttac
    12001 atgttacttt ttatctagag accttatgga ataatttagt tgagattggt aaaaacgtct
    12061 ttgagatttt aaaaaatgtt atacttgcgc cggttttgtt tattacctcc atgatttctg
    12121 gtggatggga agaaacaaaa aataatatga ttgctgtttg gaataatatt aaagaaagtg
    12181 ccataaatat ttgggaatct ataaaga
//
LOCUS       DAYYGO010000084        12143 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000084 DAYYGO010000000
VERSION     DAYYGO010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.086"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..565
                     /locus_tag="ACHA5T_002016"
     CDS             <1..565
                     /locus_tag="ACHA5T_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFM8887580.1"
                     /translation="YFKSEKDTSSLTLTLEEGVEPDKVTSKVINQLKEKGSLKHLGEY
                     EVLDTAMLTKGIGQILSTITYFITAVAGISLFIAGVGVMNMMYISVSERTKEIGIRRA
                     LGATRKSIMLQFLLEGLILTLSGGLIGYLLGMALAYSVGSLIKVNVSLDLFTILLAVG
                     VSSVIGLVFSVMPASEAAKKDLIDILR"
     gene            966..1571
                     /gene="fic"
                     /locus_tag="ACHA5T_002017"
     CDS             966..1571
                     /gene="fic"
                     /locus_tag="ACHA5T_002017"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFM8887581.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            1587..2159
                     /locus_tag="ACHA5T_002018"
     CDS             1587..2159
                     /locus_tag="ACHA5T_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8887582.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2411..2668)
                     /locus_tag="ACHA5T_002019"
     CDS             complement(2411..2668)
                     /locus_tag="ACHA5T_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887583.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(2794..3609)
                     /locus_tag="ACHA5T_002020"
     CDS             complement(2794..3609)
                     /locus_tag="ACHA5T_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8887584.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            4126..5451
                     /locus_tag="ACHA5T_002021"
     CDS             4126..5451
                     /locus_tag="ACHA5T_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFM8887585.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            5444..5794
                     /locus_tag="ACHA5T_002022"
     CDS             5444..5794
                     /locus_tag="ACHA5T_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887586.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            5776..5961
                     /locus_tag="ACHA5T_002023"
     CDS             5776..5961
                     /locus_tag="ACHA5T_002023"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887587.1"
                     /translation="MGSIRMNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSF
                     YVEAAAGYHKYEYYIGY"
     gene            complement(6865..7140)
                     /gene="prgO"
                     /locus_tag="ACHA5T_002024"
     CDS             complement(6865..7140)
                     /gene="prgO"
                     /locus_tag="ACHA5T_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFM8887588.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(7112..8065)
                     /locus_tag="ACHA5T_002025"
     CDS             complement(7112..8065)
                     /locus_tag="ACHA5T_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8887589.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            8677..10170
                     /locus_tag="ACHA5T_002026"
     CDS             8677..10170
                     /locus_tag="ACHA5T_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFM8887590.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            10305..10601
                     /locus_tag="ACHA5T_002027"
     CDS             10305..10601
                     /locus_tag="ACHA5T_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFM8887591.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            10702..11553
                     /locus_tag="ACHA5T_002028"
     CDS             10702..11553
                     /locus_tag="ACHA5T_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887592.1"
                     /translation="MKSDDWIEHNWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            11574..11786
                     /locus_tag="ACHA5T_002029"
     CDS             11574..11786
                     /locus_tag="ACHA5T_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887593.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         4171 a         1863 c         2268 g         3841 t
ORIGIN      
        1 ttattttaag tctgaaaagg atacatcgtc tctaacgtta actttagagg aaggagttga
       61 acctgacaag gtgacctcta aagtgataaa ccaattgaaa gaaaaaggta gtttgaaaca
      121 tttgggggaa tatgaagtgt tagatacggc tatgttaacg aaaggaattg gtcaaatcct
      181 cagtacgatc acttatttta taacagctgt agcaggtata tctttattta tcgcaggggt
      241 aggtgtaatg aacatgatgt atatatctgt atctgaacga acaaaagaga tcggtataag
      301 aagagcccta ggagctactc gaaaatcgat tatgctgcag ttccttttag aaggtttgat
      361 tttaactctt tcaggagggt taattggcta tttattagga atggctcttg cttatagtgt
      421 tggttctttg ataaaggtaa acgtgtcact tgatctattt actattttac tagcggttgg
      481 ggtatcttcg gttattggac tggtcttttc tgtaatgccc gcttcagagg ctgcaaaaaa
      541 agatttaatt gatattctta gataaaatca gaatgacaag aatatttacg ttctagattc
      601 taaaataaaa cacaaagaga ccaaccaggc accctattgt ttctttgtag tatagagata
      661 ttctttggct tgtaagagat caatagagtt aggtgaccgg agttcgaaat aaaggaaatt
      721 tttgattgta taggagaatt ctttatccct aatatttaga attttgagta tgccatgttg
      781 atgttttaga ttctctataa atatagaggt gtatcaaagg tcaactagac tgtgcaaaat
      841 agagacactt gaaaaatcta tcgggggtca gaaacttaat agtttttgac acataaacaa
      901 aatcgcgata taatggggtt gagagtgttt cataaactta tgtcataaag ttaaggagat
      961 ttcgcatgat tttagagaat aaattgggat taaccaatca agttgagttg gctaaggttg
     1021 aagaaaaact cagtaaacaa aaagcgaaag aattgtacga ttgtggaaaa ataaatgaaa
     1081 tcgaagttgg tacatttaaa ggtttatctg aaattcatga atttctattt tcggatattt
     1141 acgattttgc cggaaaaatc cgatcggtca atattgcaaa aggaaatttt cgatttgcac
     1201 cagttatgta tttagaacat tcgttacagc atattgatca gatgccacaa acttcttttg
     1261 aagagattat caagaaatat gtggagatga atatcgctca cccatttcga gaaggaaatg
     1321 gccgaagtac tagaatttgg ctggatctca ttttaaagga agaattgcaa aaagttgtag
     1381 attggaactt aatcgataaa gcagactatc tttctgcaat ggagcgaagt cccataaacg
     1441 atttggaaat acgctatcta atttccaatg ctctgactga taaaattgac gatcgcgagt
     1501 tatatatgaa aggaatcgat gtcagctatt tctatgaagg gtattctgag tatacgatag
     1561 atgatttgta aaagtaggtg gaccaaatga cgaaatatgg atatgcacgt gtctcaaccg
     1621 cttctcaaag tacaaaagaa caaattcaac agttgcttga gaacggcgta gagaagaaga
     1681 atattttttc agaaaaattt acgggtacaa aacgggatcg gccaaaattc aatcagctgt
     1741 tggctggtct gaagcctgga gatgaaatag ttgttacgaa gcttgatcga tttgctcgta
     1801 acacgagaga agcattagag atcattgaac ctttactcga tgataacgta acgatcaagg
     1861 ttttgaatct aggaacgatt gaaaatacgt ccatggggcg aatggtgaca cggacgttgt
     1921 tgagtgtagc agaaatggaa cgtgatatga ttgtggaacg tacccaagaa ggaaaaatgt
     1981 ttgcaagaaa gaataatcct aatttcagag aaggacgtcc gaaagcaaca atcacaccga
     2041 agaagaggca cgcatatgaa ctgcttactt caggtaaatc gtataaggaa gttgaagcta
     2101 taaccggttt cagtcgcagt acgcttttta gaataaagaa gaaaattgaa gaaagttaaa
     2161 aaagaagtct cagcgaacca gacaggtaaa tacttaatgc gttctagata tgagtctatt
     2221 agcatgttgt gaaatataat atttttttat ttacttgatt tctgctcaaa aaatgtgtat
     2281 ggtaactgta aaaggtaaag taattaaagg tatgctagcc acgcttacta tattaggata
     2341 ccatcatata tttataatga acgatttgtt cgtctaccaa acaacacata tcgttcagcg
     2401 taagtcacgt ttaggaacgt ggctttttta gtgttattat cgataaataa tctattgact
     2461 ctattgattt tatctccata tccctacggt gttgattgtc tttagatctt tcatttaaga
     2521 accaaataac tatcataaaa atcagcactg ctgctaatat aataaacagc attcctattg
     2581 tgatattacc tattgatttt accataacta cgattaaagt aataatggca ttcaagtctc
     2641 ctttgttctc tatatataaa ttagccataa atcatcttcc tttcaaatta agatacttgt
     2701 ttgataccaa taaatagtaa gcgtggctag catacctttt tctgtaatag caagtgttct
     2761 aatagtccaa acttaatgat ggaccgatgc tagttatagt acagtaatgt tttctttctt
     2821 atctagattt ttagtaagct ctatcagcaa atctaacaaa tctgagtcct tttcagaaac
     2881 taaattaaat ctagtgttta caacaaagtt ttcatgttct atattgttta gattatttgc
     2941 ggaaaggagg gcttctagat cttgacattc aaatctatat gcatcgtcat aaatcttact
     3001 agaaggagtg cttaattcga aattacttat tttctcatct tggaccaaga taaatttata
     3061 gatagtatct ttttgtgttt ttcttttaaa aacacaacag agtgtgttat aaggaacgtt
     3121 ataattttca tcagcctcat tttttctcca ttcaaacaaa tgataaactg caaccaagtt
     3181 gccatcaatt tcatggacag tgctctcggt aacatccttt aaagaactat caagggctaa
     3241 tttatagtaa ctagagagag gaagtaaaat tgtcgattta ctgagatggt tatcgtctat
     3301 acgtgcaatc atacctaaat caactaaggt tttattccag gtgtttatag ttttagttgt
     3361 cactcctaga gactctgcta tggtattgac cgaatgccag gaatccccgc cttcattctt
     3421 tgccttaaaa cagtaataca gatataaatt aatagctttg ttattcggta aatatttttc
     3481 aagttctgtt ggtagaataa agtaagaatc cttaatgttt tttctgaact gtctccagtc
     3541 tctgtagttt tgtttataaa gttgtgaaac ggtttttcca cctttagaaa gtcctgctat
     3601 cttaaccaat cttattgctc ctttcttgaa tctgtcatga tcatacagta ttttttactt
     3661 gtagtccagc accttaatat attatcctat gtaattaatt tcgtactaga tagtaattat
     3721 tttttattta attctctagt cacttacaaa taagttatat aaactacgat taataaatta
     3781 ttacaaaaac tattaagaaa acttctacat acaactatta caaaactatt aagaaactga
     3841 atactcctat tttttgtttt taaggaaagt tttttctttt ttgaatttct tatttttttc
     3901 taatataact aagcaaaact ggataatacg acacttattt tcaaattttc acaagactaa
     3961 tagtaaatat ccatttaata aagtgataca atgtgttgat tttacaaatc aacagaagaa
     4021 gtagggaaat aacttaccta tgcttctttt tttgtacatt taatcgattc ttctttacaa
     4081 aaacgaacgt atgtttgtat aatgtggaag aatggagtgt tatttatgat ggattattca
     4141 aaagagccag taaatgatta ctttttgatc gatatgaaaa gcttttacgc aagtgtcgaa
     4201 tgtatagaaa ggaatttaga tccattaacg actgaattag tcgtcatgag tcgagcggat
     4261 aatacaggat cagggttgat tttagcttct tcaccagctg caaagaagaa gtacggtatt
     4321 tctaatgtca gtcgaccacg agatctaccg caaccttttc ctaaaacgtt acatgtcgtt
     4381 ccaccacgca tgaatctcta tattaagcga aatatgcagg taaacaatat tttcagaagg
     4441 tatgtagcag acgaagattt attgatttac tcgatcgatg aatcgatctt aaaagtgacc
     4501 agatcaatga atctttttac gactgaagga acaagaagtc aacggagaaa gaagctggct
     4561 caaatgatcc aggagaatat taaagaagag ctaggattgc ttgctacagt aggtgtcgga
     4621 gataatccct tattagctaa acttgcttta gacaatgaag caaaacataa tgatggattc
     4681 cttgctgaat ggacgtatca aaatgttcct gataaagtat ggaatattcc tgaaatgaca
     4741 gacctttggg gtattggatc tcgaatgaaa aaaagactca accaaattgg tatcatgagt
     4801 attaaagact tagctaattg gaatccatac actattaaga gccatttagg tgtgattggc
     4861 ttacagctgt attttcatgc gaacgggatc gatcgaacag atatagcgat cccaccagaa
     4921 ccgacaaagg aaaaatcata tggtaattca caagtgttac cacgagatta taccaggaga
     4981 aatgaaatcg aactggtggt aaaagaaatg gcggaacaag tagctattcg tatcaggcaa
     5041 cataactgta aaacaggttg tgtccattta tacattggca cgtcgatctt agaaacgaga
     5101 ccaggttttt cacaccagat gaagatccct ataacagata atacaaagga attgcagaat
     5161 tactgtcttt tcttgtttga taagtattat gaaggacaag aagtaaggca cgttggaata
     5221 acctatagca agcttgtgta cactgattcg cttcagctgg atttatttag tgatccacaa
     5281 aagcaaattg acgaagaaaa tttagataaa attattgaca agattcggca aaaatatggt
     5341 tttacgtcga ttgtacatgc ttcttctatg ttagaaggcg ctcgtagtat tactagaagt
     5401 acattggttg gtggccatgc aggcggcgca ggagggctta aaaatgattg attataatga
     5461 tcgtggacgt ttaaagtggg caggttttta tttatctgat catactgaaa aaattgatgc
     5521 cgacaatgaa caacgagcca atcaaaattt ggcaaaagaa caaatgacga ctgaagaggt
     5581 tgcggaagta ttgaacgctg cagtcataaa aaatagatca gtatgtattc aaaaagaaga
     5641 acgaaacgct gaaggatact atccagaaga tattgttagc aagatcttag gtagtgatga
     5701 gctggggatc tacgttggaa aaaacaaagt tcactacgac gaaatacgtc atatcgaatt
     5761 ttaccaggat aataaatggg gtcgattaga atgaacatta tccagcaata tgagctaaag
     5821 tatataacct ttgatcagct atcagaagag atttggggtt atggccagcg tttgataaat
     5881 gaggttggtg ttgaacgctt ctctttctat gtagaagctg cagcaggata ccataaatac
     5941 gaatactata ttggctatta aagtgcaata aaattatatt tcttttatgt tttacatttt
     6001 gtatcaattc tgataaaatt acctataggt aattaaaaaa tataaaaaaa ctagttacaa
     6061 ccttaggacg ttgtaactag cttgaagcat actagagcta actaaaatag cactagaaag
     6121 caatgttaaa cccgtccaac aaacttgatt taacattgag accagcttat tcagctaaat
     6181 atagttgaat aagcttcttt tgttttaaaa aaacaaaagg tgtactacga ataacgcaag
     6241 taaaagaaat actattacaa ccagtaggct acaagtaagt attactaaac attgcgtagt
     6301 tagaaccttt gctctattgt ctaaagccac aaaattagag atcagcttta tgatgctaag
     6361 agcgagctgc atccatgcgg acataggttc ttttgacact attaattctc ctttttagga
     6421 taagggaaat tgctagtagt tttttcatta gcaccatccc ccttcatttg attgagtcta
     6481 gtgctgctgt gatagtttgc tgtttaatat gcttacattc ttattataac ataaatgaaa
     6541 caaacacatg gttagaggtc gtatcaatca aaatagtgtc tcacaaacga tttattactt
     6601 actcctgctg actaacggac tgttcttcag taactgtaac tatatgtttg ttaaaaccac
     6661 cattacctgt agataaaaac caatatattt actatactca ctatattaaa tatatttagt
     6721 atagtaaaaa ctagaaacaa taaaatttgt gaatgttgta tacccccaaa caatacacat
     6781 attttgaagg attaggatta ataaagctaa aaatatagta agtatactaa atatatttac
     6841 tatatctagt atacttacta tattttaatc ttttgatcgt ctattatata taatccgttc
     6901 ctcgtcgttt aaagtatcaa cataggcgtc cattgcaagt ttgactattt catacatagg
     6961 aatatctttt gcataactaa gatcccgaat ggtttttagt acgggaggat ctacttgaac
     7021 tggttttctt tctttcgcag tgtactttcg ttcccctaac tcgacattaa tatcatcttt
     7081 aattgaaata tcttcagcac caattttctt cttatctttg ttcgttggta ataatgccat
     7141 ttagtttaat ctcctttcca agaggtagga tatgattggc cattgagtca gcatatttag
     7201 gtgtataatc aaatccttca atatcaccat gttgttcata gtattcaatt ctttcttcta
     7261 attctgtaaa gatgtctgca aaaatagccc accatcttcg atctacataa tcatttaatt
     7321 ggataccatt ctcaccatac atttctaacc gatccgagcc tttaacaatg cttttaaaaa
     7381 tgtttgattc accatactta tcaattgttt tttgatagtt tgcgtgttga gaggaacgtc
     7441 gcactgagaa aagaacaggc aaaattccaa tgatttgaag atgagactct tcaaaattcg
     7501 tgacaatcgg aactaaaact ttattaataa aatcttctgt tccttccatt gcgtaccgtt
     7561 ttaattcttg cattgcaata atataatctg cagcagttaa gaatgcacga accacattat
     7621 ctgttgatgg tgggcagtcg ataaagatat aatcgaaatt ttttctcaat tcttctaaag
     7681 gtttaatgaa ggccaatgat ctcttacgac gatcaggata tttttcggac aagtattcat
     7741 taagttcttc agatccagga gagccagcca taatatataa atgtggactt acttgagtaa
     7801 tacctgaaac ccagtcccct tttttgattg ctgcagacat ggatcgatca aagctagtaa
     7861 tttgatacgt tgatccaatg ttggaagtag tatttctttg ttggtcataa tcaattagca
     7921 acacgtcttt ttcaaaaagt tgactagaaa caatcgctaa taaatcattg tctgtagtct
     7981 taccaatgcc gccttttcta cctgtaagca ttacaacttt tcctagttta tctgatgttt
     8041 taaatgcagt ccgtaagttt tccatagttc cctccaattt cctatactaa atatatttag
     8101 tatacttcat atacttaata tattcgatat atctaatata ctccaaattg ataagaattc
     8161 agactttata aggaatatag tggatatact aaatatattt gctatattca gtatatcaga
     8221 tatattcaaa atgtcaaaat atatttgtgc gttttgcgtt gacaatttag agtgaaaaga
     8281 ggtaaactta attctataga aaccacagat aattcactat ttacaaagta gcaaaagcag
     8341 tggtactctt taaacagaaa aattacataa aaaaaagaga cggaaataat tgattttagc
     8401 agaatcaatt atttctgaat cacatggtta ggacatgtgt tcacgctcgt ctcaagaata
     8461 tgtaagtagc taccaactac ttatattaaa agttgatcgg ataccttttc cgattgcctt
     8521 tattctacca aaaaaaggcg agcaactcaa cagaagaacg tttatttagt gtacttaaat
     8581 taaatattct taaacactga agagagaaca catactatgg ctgagattaa agctaaactg
     8641 tgttctcttt ttgtttgaga aaggacgtta caatcgatgt tgagcgaaat ttattcagct
     8701 attcttaaaa acggcttacg tacatacaaa tatcgaaaca gcaccatgaa acctgcaggt
     8761 catattgatt ccagtaggag tggtgctatc tttgcgtacc gtagcaagca attaatgaat
     8821 gttgggcgtg gtatggtcat tacttcggaa gaagctattt tagagaacga aaagaaacta
     8881 acgcattgga cacctaatac ctatcgcttt ggtacttatg cggatgatta tcgaacagtg
     8941 gttaaaggtc attctgagaa aaacttaagt caaatcaaca catttgtagt tgatatcgat
     9001 tcaaaagaga accatcaagg cgaaattatt ttagcctgtt tagatcaagt gggctacatg
     9061 ccaacgctaa ttttagaatc agatcatggt tatcaagttt attttgtact aaagacacca
     9121 ggctatgtca caaagaaaag caatttcaag gtgatcgatg tagcaaaaaa aatttcaaaa
     9181 acaattcgtc tacagttggc tgaaaaattg tccggagtgg atctaggatg taaccacttc
     9241 ggaattgctc gattcccaca agaaaataat attgttttta aagaaatgaa ctatcagtat
     9301 tcttttgctg actggattca atggtcgatg aagcttgaat cagatcaaaa aacaaaagaa
     9361 gatcgagaac gaaaactaat tgtctttcct gagaaaaaag aaattcgtca agtagatgaa
     9421 ccgtggttcg atatgttact tcataaagca aacatcgtcg gcggagaagg acgtcttggt
     9481 cgaaataatg tgattttcac gctttcttta gcttattact cttcaggtta tgcattggat
     9541 acttgcgaat acaatatgtt cacattcaac gaacggttac aggaaccgct atctgaagca
     9601 gaattagtaa agattgtacg tagtgcttat tctggtaatt atcaagcagc aagcagagaa
     9661 ttcattattg aattgtgcca gcaatgggtt tcaccaacct taaaaaataa tgatctgttc
     9721 attcaacgac gtggctggtg gaaatttaaa aagccacgag atcaaagaaa atatagtcat
     9781 aaaaatgaat ggaaagaaga tctattggct tatctaagcg aaaaaagtct tgctagtgag
     9841 ccttatttga acctgtcaaa aaaagaattg gcttcacaac taacgatgcc caagcgaaca
     9901 cttgataaga tcttaaatga gttacgagag gagaataaag tcttctacaa ggtaacacga
     9961 ggaagaaatg gcggaatgat cattgcttca gtcaaaacgt tgttcgcgag ggttattcaa
    10021 ctaagtaaag aaaagagact agattattca acaaggatca cagaggtatt tggactcaac
    10081 gagagcttag tagaagagac atttagacaa ttatcaaccc ccttgaaccc tcttaaagaa
    10141 gtgaatcttt ttgaagtaga tacaggttga aaaataaaag gtgcaaattg ccttatacaa
    10201 tatatctatg atacttctgt aggtgttgct ccctttgcct agctacgtgt aggtggtata
    10261 cttataactg cgtatgcagt cttatgtatc atggaggaag ttgtatgtcg ttaaaaaatt
    10321 atgtctattc acatcctgtt aatgtgttta tcattggtcg tttgggatta tctgtggaag
    10381 tgttctgtga gctttatggt tttaagcagg ggactttgtc ttcttgggtt accagggaaa
    10441 aaacagttgc ttctttgccg attgagtttc ttcatgggtt gtccttagct tctggggaga
    10501 ctatggatca ggtatatgat tgtttgtgtg tgttagagca ggagtatatt gagtataaaa
    10561 tagctaacga gttaagaaaa aggaagaagt atatccagta gtagttttta tttacttaaa
    10621 aaattggttc attgagcaac ctatcgctgc taaagtagca aaggaaaacg ttaaaggaaa
    10681 gaatttaggg aagaaatcga aatgaaatcg gacgactgga ttgaacacaa ctggcttcct
    10741 ctgaaaagaa atagcaaacg aaagaaattt tgggtattgc aattatggcc attaggaaaa
    10801 ctttgtagtt acggggcacg acttattctt gatttaaccg tttactcgat tggagtattt
    10861 ggaacggctt attacttgat aaatgatccc aataggattg cagcatcaga catttttaca
    10921 gtgctaatga cgttgatttt tgtcatgcta ttgtctattt ttactcaatt agtcatgcaa
    10981 tttaccaggc aaaaattaac agcaggaaaa cttttcaaaa gcctattcga tttaattgtt
    11041 gtaaatgcaa ttctagcgac tttaataaaa caaattgaac aatctggaag aacaacaaaa
    11101 ggagtgaagg attttttatt gacgtttatt cactttgtaa atggacacat gtatttgttt
    11161 gaagtgggta ttattctctt ttttgtcggg tatgtatcca cgaaacttat taaacgttca
    11221 attgaaaaag aaattgaaat tgacctgatt ccgacacttc ttggttatga gtacaatgtt
    11281 gattttggaa gatatgacga attgatcaat acggatatcg ttcacacgag tatccatgaa
    11341 tctactgtta agggcatcag atctatttta aaaaatggcc aggaaatgaa agcaatagat
    11401 cgaagaaaag agatctacaa tttttatgaa gtcgaagcaa gcctacgata tggcatcgga
    11461 aaattgtggt tagatggaag taatattcgt caaaacaaac catacatttt ttggaacagt
    11521 agtgatttag aacttccaat gaaagataaa taattatgga gaatcgtttg aaaatgattc
    11581 ctctatacag gattgtgctt atagttggca tggagttaaa aaccttaaag aaggagcagt
    11641 tattcaaagt gaaagaacgt cataatcagt tactatgtag taaaacgggt acgatgaaca
    11701 ttgaataccg aacagaatat ttagaattaa cgaatcaaac tgattttttg ttgattcgaa
    11761 agcaacaaga gcaagaaaaa aattaatggc tacttatagc caattgattg atgaatggga
    11821 acaattcaag gaatctaaaa ttccagaacg tctctaaaag cctttatttg taaagagcat
    11881 aaggaaacgt gggaacactt tttaaaaaaa gtgttccaat aggctgtgcc tattacgttt
    11941 ctcaaaaata aatgtgggaa caaaatattt aaaaatagga tagtgaaagg atagtgaagg
    12001 atagtaaaag gagggtagta ggatagcaaa aaggataggc tccttttcgc tatccttttt
    12061 aaattggagg aattaatatg gaagtaagaa taagaaatgt ggatgagaaa atcgtaagag
    12121 aaattgatcg aagatgtgac gaa
//
LOCUS       DAYYGO010000085        12125 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000085 DAYYGO010000000
VERSION     DAYYGO010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12125
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.087"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(87..251)
                     /locus_tag="ACHA5T_002030"
     CDS             complement(87..251)
                     /locus_tag="ACHA5T_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887594.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            546..1874
                     /locus_tag="ACHA5T_002031"
     CDS             546..1874
                     /locus_tag="ACHA5T_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFM8887595.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2076..2909
                     /locus_tag="ACHA5T_002032"
     CDS             2076..2909
                     /locus_tag="ACHA5T_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFM8887596.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2925..3662
                     /locus_tag="ACHA5T_002033"
     CDS             2925..3662
                     /locus_tag="ACHA5T_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFM8887597.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3817..4785)
                     /locus_tag="ACHA5T_002034"
     CDS             complement(3817..4785)
                     /locus_tag="ACHA5T_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFM8887598.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5017..5850
                     /locus_tag="ACHA5T_002035"
     CDS             5017..5850
                     /locus_tag="ACHA5T_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFM8887599.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5884..6723
                     /locus_tag="ACHA5T_002036"
     CDS             5884..6723
                     /locus_tag="ACHA5T_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8887600.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6749..7309
                     /locus_tag="ACHA5T_002037"
     CDS             6749..7309
                     /locus_tag="ACHA5T_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8887601.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7508..9229
                     /locus_tag="ACHA5T_002038"
     CDS             7508..9229
                     /locus_tag="ACHA5T_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFM8887602.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9394..10536
                     /locus_tag="ACHA5T_002039"
     CDS             9394..10536
                     /locus_tag="ACHA5T_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFM8887603.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10552..11763
                     /gene="thiI"
                     /locus_tag="ACHA5T_002040"
     CDS             10552..11763
                     /gene="thiI"
                     /locus_tag="ACHA5T_002040"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFM8887604.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4130 a         2035 c         2520 g         3440 t
ORIGIN      
        1 tcttttatta ttatgatgaa tcaagctttc tgtttttgtc acgtatgttg taatctttac
       61 gaaagagtag agttggctac tcataatcaa ccatacttcc catcatcgct aaatacgaaa
      121 taaatcagca ttcctacacc aatcaaagca caagctgcac caagaaaagg gatgacattc
      181 ccaaagttat ctgtattcat aagtatgcca ccacccacaa acaaaaagcc gccagcaatc
      241 aggtttttca ttttattcat ctcctttctt acgttatagt atttccttct ttcaatgagt
      301 attttgaaag ctatcttatt gtatgatgtt cttctaggcc tcttctttat tatacaggcc
      361 attttcgtta tctcatagaa atattccttt ttatctgaaa actattgtat accgtatgac
      421 atttttcttc atgaagttgt aagtatattc tcattcaaat gaaaatttca tcatagaaga
      481 gattgtattt ctaaattctt aagagtaaaa tacgaagtat aacttggatt tggaaggatg
      541 attggatgaa agctattacg cctgatatga caacaaaatt taacaaacag tttcaagcaa
      601 atatcaaaca gcaggcattg cagcgggcag ttgtgaagaa cggaattact gcttctgctg
      661 aaaatgtttc agcacatgtg gagaatacac cggtattttc tatagatttg gctacgggga
      721 aagtggctaa tcaaatgcaa tcggggcgtt gctggatgtt tgctgcactg aacacattcc
      781 gtcataagat gctgaatcaa ttccatttaa aagagtttga attatcacaa aattacactt
      841 ttttctggga taaatatgaa aaggcaaatt atttctatga aaacatcatt gcaacaggaa
      901 aagaggcaat cgatagccgg aaagtagctt tcttactcgc tactcctcaa caagatggcg
      961 gccaatggga tatgatcgtt tcattattcc aaaaatatgg cgttgtgcct aaatcggtga
     1021 tgccggaaag tgcgaatagt tcaaattctc gagatttgaa taactacttg aacaaattac
     1081 tgcgtaaaga tgcagtagtg ttacggaaaa tggttgctca aggagaaaca ttagacgcta
     1141 ttgaagaaaa gaaagaagaa atgctagaaa gcatttataa cttcttagct atctcattag
     1201 gaacacctcc aaaagaattc gattttgagt accgcgatga agaaaagaac tatcatcttg
     1261 accgaggact gacaccacaa attttttatg ataaatacat cggtgtaaaa ttagatgatt
     1321 atgttagcgt gatcaatgca ccaacaaaag acaaaccatt caatcgttct tatacagtag
     1381 agatgctagg aaatgtcgtt ggcgggaaag aagtcaaata tctgaatgtt gatatggaaa
     1441 ctttcaaaaa attagcgatt gctcagcttg aagaaggaga atctgtttgg tttggttgtg
     1501 atgtaggtca atcttctact agaactagtg gcatcatggc cttagatgct tattcaatgg
     1561 atgatctatt tgatacggat tttacaatga ctaaagctga acgtttagat tttggagaaa
     1621 gtctgatgac ccatgcgatg gtattgaccg gtgttgactt agtagacggg caatcaacaa
     1681 aatggaaagt cgaaaatagc tggggcgaaa aagttggaaa gaatggcttc tttgttatga
     1741 gtgatgcctg gatggacgaa tatacgtacc aaatcgttgt aagaaaagat ttattgacga
     1801 aagagcaatt aaatgcgttc aatgaagaac caattgttct atctccgtgg gatccaatgg
     1861 gtgctttagc atagacgagt atttcaatga agagtatgta gtaaagataa aaagagctgg
     1921 cttaagccag ctctttttat ctttaaagtc cattccctgt tttttatggt ccctgtgcta
     1981 taattctaaa taaaggataa ggattggaaa taatcctttt atcattaaaa atatgatgac
     2041 gaagtagaat aaaattggga gtagggggga aagtcatgga tatccaaact gttttatctg
     2101 atctgaaatt aaatggtaaa gtgattccag ttgttggtgg cgatgttaat cagacctatc
     2161 gtataaaaac cgaacatcgt gcttattttt taaaaatcca tccgaatgta aaaaaaggat
     2221 tttttgaagc agaagtagac ggattaaaag aactgtcagc attcgtacgt gtaccggata
     2281 cgtatatgct aggtgaaaca agtgaaggcg cttatttgtt aatggaatgg atcgagccgg
     2341 gaaaagggga tcaacgagat cttgctgcag ctttagctaa tctccatcag caaacagcgc
     2401 cacaatttgg ttttcgtaaa gataactatt tgggtacact tgttcagaaa aattcttttg
     2461 aagaagattg gtggactttt ttctttaaag atcggcttga atcccagatt tctttggcag
     2521 aagaaacgaa tcgatggaat gtacagcgtc aagaaaagta tctgcgattt aaagaacgtg
     2581 tattgaaatc ggttgaacca aaaaagatca ctccacgcct cttgcacgga gacctgtgga
     2641 gtggtaatgt gttctttgac cagcaagggc atccagtttt tgtcgaccca gctgtttctt
     2701 atgggaatcg ggaacaagat attgcaatga gccagttatt cggtggtttc cgaccggaat
     2761 ttttagatgc ttatcaaact atatttccat tagaaaaggg atggaaagac cgactgccga
     2821 tttaccagtt atattatctt ttggcacatt tgaatatgtt tggagaaagt tacggttccc
     2881 aagtagatca attactagaa aatttttaag aggagggcgt gtttttggca aaaaaatata
     2941 ccaaaaagca tgaagtttca tattatgagt gtgacatcaa ccaaacgatg acatttcctt
     3001 cgatgttagg agtagttatc aaaacgtctg aagatcaaag cgaccagttg ggaagaggca
     3061 gtgaattcgt caatagcttt ggacttactt gggtcatcac gaattattcc atggagatta
     3121 atcgtctccc tagagtcggt gaagtaatct cagtaacgac tcaagccatg gagtacaata
     3181 agtttttctg ttatcggaac ttctggatcc atgatgaaga tgggaaagaa ttagtcaaaa
     3241 tagaatctgt tttcgtattg atggattttg tgaatcgaaa aatgagcagt gttaatgaag
     3301 agatcattgc tccttttgag agtgagaaga tcaagaaaat caagcgtcag gaaaaaattg
     3361 aaaagattga ttcgggcttc atgttgccat atcgtgttcg tttttatgat atcgatagta
     3421 atcagcatgt taataatgcg atgtatttta attggatcat tgacgtatta ggatatgact
     3481 ttttgactac gcatgttcca gaaaaagtaa ttattcgatt tgataaagaa gtagagtacg
     3541 ggaatgagat cgaaagccat tttgaacaaa tccctatcga aaatggtgtc aaaacaaaac
     3601 atgaaatccg aatgcaagaa cagctgtatt gtgaagcaaa tattatatgg aaaaataaat
     3661 aaaaaagagg ttgtgacaga agggggcaac ttcaagaaat aagaagaaat ttacgaaaat
     3721 tgtttctcaa atttttgtaa attttcgact tagttttcga agaagctatt tttgtcacag
     3781 cctctctttt taagtttagc tattagataa tgatgtttag ttttggataa acgaaatcaa
     3841 ttcttcaccc gacaaaccat agtttacagc aatcaatagt tttaaacgtg ctttttgact
     3901 attaatactg ttacagaaaa taatccccat tttttctagt tcttttcccc cgccttcata
     3961 atcataaaca ggctctgcga ttccattgaa acaacgagaa acaagtacta cagggatttt
     4021 ttggacaaga agcttttcca aagcagctaa ggcttgtgga gggagattcc ccgcacctaa
     4081 agcttcgatt accaaaccgt ctactttcgt atgggcgatg gcttccaata agtccccttg
     4141 catcccagca tatgctttaa cgatcgggat agtcccatct acagcggaaa tgtccagtcg
     4201 ctttgtttct aaaagttcct gcaagaataa aattcgattc ttagttacta agccgatggg
     4261 acccaatgta ggtgttcgga aagtagcaac atttgtggta tgggtctttg tcacgtagcg
     4321 tgcagaatgg atctcatcgt tcatgaccac caaaaccccc ttatctagcg cttcttcaca
     4381 acttgcgaca cgaatcgcac tttcaaaatt ataaaggccg tcacttccaa gttcattact
     4441 tgaacgcatt gccccagtca aaacaattgg taatttgttg ccaatcgttg tatcaagaaa
     4501 aaaagcagtt tcttctaatg tatctgtacc gtgtgtgatc accactccgg atatacctga
     4561 tgcataagcg gacaagatac gttcttttaa taccaacatg tgagaaggca tgatgtgtgg
     4621 tgagggaaga ttgaagatat cttcaactaa taattctaca tgcttgggga ttgtgatttc
     4681 tgcatctaat aatgggtttg ccacatcagg actaacaccg cccgtgagca tgttttcttt
     4741 catggcgatc gttccgccgg tgtgtagtac aagtattttt ttcattttac agacttcctt
     4801 acgaataatt ttgcatagcc gttaaatctt attgtatact atataaaaga gttttgctat
     4861 cagtgaagaa taacgaatag cctaacttat cataaaaaag ctgaccttat taaaatggaa
     4921 aagactcaac cttgacctcc taatacaatg gagtatctgg ttgagtttct tttacaagaa
     4981 agcagccaag gaaatcaatc acgaaggtgg ccggaaatga tcagaatcgg attaacatcg
     5041 tttaaagaac atgacaaatt aactggtaaa aagaataaca ctctttatga atatgcaggg
     5101 tttctaccct tagtggaatt agatactgcc tattatggaa ttccccgaaa gtcctcagtt
     5161 gagaattgga taagtgaagt acctgaatca tttcgctttg tagtcaaagt ctatcaagga
     5221 atcagcggtc aaggagactg gaagccttat tatcagaccg aagaagagat gattgcgcat
     5281 tttttagatg ttatgtcgcc aatggtagat gctggaaaga tgttttgctt tttagtccaa
     5341 ttcccctcat cattcaaatg tacgaaagaa aatgtccagt acttgcgcaa acttagaaag
     5401 tggtttgagg gttatcctgt ttctatagaa ttgcgggatt attcttggta cagcgatgaa
     5461 tttcttgaac gtacaagaaa ctttatgtct agagccgctt tttctttagc catcatcgat
     5521 gagccacagt tgctgaatac aacgattcct tttgatccat ttgtcaccaa tccgaacttt
     5581 gctctttttc gattccatgg taggaatgcg caaggatggc aagcaaatga taaagagtgg
     5641 cgtaaaaaaa gaacgttata tcgttattca gaaaaagaac tgcaagaact agcacatgaa
     5701 gtcaaagtag ttgattctaa gacaaaagag gtaggtgtga tttttaataa taattctggt
     5761 ggtgatgcgg ctgaaaatgc attagcactg caaaaaatat taaatatcaa gccagatgga
     5821 ctcaatccaa cccaaatgga tctgttttaa gggcttattt tattcaagaa aggggcgaag
     5881 tctatgatca aagctatttt ttttgatatt gacggaacat tggtcaccaa tcgttcaaaa
     5941 gcgttggaat caacaaaaca agcaattgaa tatgctcgga aaaacggcat tttatgcggt
     6001 gtagcaacgg ggcgcagccc agtaaagatc aaagaaatca ttgatgaatt ggaattagat
     6061 atgtatgtcg tgtataatgg tcaactggtt tttactgcag atcgcacgat tatcgaccac
     6121 ccttttgaac aaaaagtatt ggaacatatt gttgaatttg cagatgagaa ccatcgacaa
     6181 atcgtttttg gtgcgagaaa tcggttagat ggatcaacga cgatgctatt aggacaatcg
     6241 atttttatca agcgactagt cagcttttta ccgcgtaaat tcccggtacg tctgatgaaa
     6301 aaaatcctcc aggtttttag ccctcaccga caaaaggatc gttatgaaac gttagagata
     6361 ttaaaagaac caatttatca atgtatattg ctcagtccgg agtcagaaca gaaaaagtta
     6421 gaacatcgct ttcccgagtg tacgtttcag cgttcaaata cgtatactgt agatatcatc
     6481 ccaaaaggtg gttcaaaatt actaggcatc caagctttcg caaatgctgt cgaaattgaa
     6541 atggaagaaa ttatggcatt cggcgatcat tacaatgata tcgagatgct gaaaggtgta
     6601 ggaattggag tggccatggg gaatgctcag atcgaagtaa aacatgctgc agactttgtg
     6661 actcaatcaa atgaagaaga tgggatatac gaaggactga aacacttcgg tctgatttat
     6721 tagtcaatga aagaaggaaa aaatcagtat ggaagcagag acaccttttc aaaaagtaga
     6781 aacattccac aaaacttttg atccaagaag accagagata cccacggctt ttacagaaaa
     6841 tgaagctcat acacgtgctg cgttcaaaat agaagaactt gttgaatttg ttgcagctac
     6901 ttcacctaat aaagaacaac tgcatgaagc tgttatgctg ctgcacgaag cattagataa
     6961 agcagaagaa aaaatacttg gtaaaacaca gtggggaagt gatccgctgg ttgaacaagt
     7021 agatgcactg acagatatcc tttactttac ttatggttct ttttcattac taggaatagt
     7081 gcctgatcga atattcgaaa tcgtccatca ggcaaatatg gggaaattat ttccagatgg
     7141 aaaacctcat tatgaccctg taacaaataa agtgttgaaa cctgaaaact ggcagatgga
     7201 atatgctcca gaacctaaaa tcgcggcaga aatccaacgt cagattcaag aagcgattgc
     7261 taagaagaaa tccgctgata taggcatgtg cacaaatgaa aaagagtgat cagagtttca
     7321 ctatttttgt tatctttata cgatggaaaa acaaaagatt ttcgtttaag ttagtgaatg
     7381 catgaaaata gtatgctata atctagaata gatgtctttt gcacgagaga cgtattattt
     7441 ggatttatgc caagcagaac gccatctgtt gcataaaaga tgcatactga aaacggagga
     7501 accataaatg ggaaataatg ttatcatcgg aatcgtcatt gcaatcatta tagtcgccat
     7561 tgtcgtcttt ttggttagtt acttgatgaa gaaaaaaaac caagatcgat tggatgcctt
     7621 agaaaaacgc aaggaagaat tatttgactt gccagtgatt gaagaagtcg atgatgttaa
     7681 aaaaatgcat ctggttgggc aaagccaaaa tacctttcgt gaatggagtc aaaaatggaa
     7741 tgacatatct acttcctcgt tcgctgaact agaaagtcaa attttcgaag tggaaaacct
     7801 caatgaaagt tttcggttct taaaaggaaa aaaagcagtt gttgaagcag aagctacaat
     7861 ggaccaaatg gagaaacaag tgacggaaat ccgtgaaggt ctaaaggaat tgcgggaaac
     7921 ggaagaacgc aactcactag ctgttcagca ggcacttgac gtctatgaag aactgaaaga
     7981 aaaagtcaga gaagacgagc atgaatacgg accagctctt tttgaattgc aaaaacagat
     8041 caaagtgatc gaaaaagaat ttacgcagtt tgtgacttta aacacttctg gagaccctgt
     8101 tgaagcccgc gaggttttgg ataaagcaga aaaacatacg tatgaagtgg aagctctgat
     8161 gaaaaaaatt ccaccgctgt atgaggagct ccataccact ttccctgaac aacttgaaga
     8221 aatcagtgat acttacgaga aattgatgga agaacaatat gtattcccag aagaagatct
     8281 tgctgaagat atcgcaaaag taagtcgaag aattgaaaat tcattagcca atcttgaaaa
     8341 aacagaagtt gaaacggtcg aatttgaaaa ccgtgaaaca gctgatttga ttgattcgtt
     8401 atatgatatc ttagaacggg aaatggaagc tcagagatat gtaaaaacta accaatcaac
     8461 gattgcagag tatatcaaac atacaacaaa gaataaccgt caattattga ttgagctgga
     8521 tcacacatct caaagctata cattgaacca caatgaaatt ggccgtgtac gaggtttcca
     8581 aaccgaagtg gaagaaatgg aacgtcaaaa tgaacagatg attccacaga tccgtcaaca
     8641 cgaaattcct tattcagaag tgcgcacgtt ctataaaaca gtcttcaaag tcttagaaga
     8701 tatcgaaaag caacaagtag agatcgacga ctcattacat gaattgagaa aaggcgaaaa
     8761 agaagcgcaa gaaaagatcg atacattcga attcaaattg cgcagtttga aacgttttgt
     8821 agaaaaacaa cgtttgcctg gcttgccgaa tgattaccta gaattctttt ttgttgctac
     8881 tgaccgtatt gaagaactaa gtgttgtctt gaataaaatt cgagtaaaca tggaagaggt
     8941 caatcgttta gttgctctat gtgaagaaga tctagaatta ttagataaga agacacatga
     9001 tttagtagat gcagcagcat taacggagca aatgctccaa tatgcaaatc gttatcgtca
     9061 tacacacgaa gaagtgagag aagcaattga taaaagctac tatttattca ataaagaata
     9121 tcactatcaa gaagcattag acgaaatcgg tacagcatta gagcgagtag aacctggtgc
     9181 tttcaaacgc atcgaagatt tttattttaa tcatccagac ttagtataaa gcaaaaaagt
     9241 tagtaaacca gcaagagcaa gacggattat tgtcttgttc ttgtcttttt ttgtatattt
     9301 gaaaattgaa acaagtgaaa atttcacctg ctcttgaaag caccttggac aaccgttata
     9361 atagaaaaga atttcaaaaa agaggggttt cttatgatat attttgataa cagtgcaaca
     9421 accccaatct ttccgcaaag cttagatgca tatgtaaaaa ccagtcagcg gattattggg
     9481 aatccatcaa gcttgcatga tttaggcaat caagctaacc ggttgttgca gcaagcaaga
     9541 aagcagatcg ctgatttgat ccatgtagaa ccagaagaaa tctattttac ttcaggcgga
     9601 acagaaggag ataactgggt attaaaagga acgatgatcg aaaaaagaga atatgggaat
     9661 catatgatca tttcaggagt cgaacatccg gccgtcagcg agacagcaga acaattaaaa
     9721 gaattaggaa tcgaagtaag tatcgcccca gtggataaac gcggatttgt acgtgtcgac
     9781 gaattgaaag aattaataag aaaagagaca gtattagtct ctgttatggc tgtgaataat
     9841 gaagtaggat cgattcagcc catccaggaa atcagtgatc ttttggatga ttttcctaaa
     9901 attcatttcc atgtagatgc tgttcaggcg attggaaaag taccattctc gcaatggttg
     9961 acagaaagag ttgattttgc aacattttca gctcataaat tccatggccc acggggaact
    10021 ggtttcattt attggaaaaa gggtcgtcgt ttagcaccat tactgacagg tggagggcaa
    10081 gaaagaaatc agcgaagtgg gacagaaaat gttccaggta ttgtggctat ggttaaagct
    10141 ttacgtttat cattagagat aaaggaacaa aaacctgagc agacacaacg cttaaaagta
    10201 gctctaatca atgcgttgaa gacgtatgag aaagtgacga ttttttcaga aggaactgac
    10261 tatgcaccgc atattttatg ttttgctcta aaaggaattc gcggcgaagt tctcgtccat
    10321 gccttagaag aaaaacaaat atttgtttct actactagtg cctgctcctc acgtaaacat
    10381 atggcaagca gtacattgca cgcaatgcat gtaccaaatg atctggctac atctgctgtt
    10441 cgaatcagct tagatgaaaa caattcgctt gcagaagtcg agcaatttat gattattttc
    10501 aagcagcttt ataagaaatt ttctgcaatc aattaaaaga ggagtgttct tgtgaaatac
    10561 agtgaaatta tggtccgtta tggggaactt tcgacaaaag gaaagaatcg aaaaagcttt
    10621 atcatgcaac tagcacaaaa tgtcagacaa gctttgaaag actttccaga aatcaaaatc
    10681 catgctgatc gggaccggat gcatctttta ttgaatggcg cagacgggca gctagttatt
    10741 ccaaaactag caaaaatatt tgggatccaa aatttctctc caagtattcg tgtagaaaaa
    10801 gatgtcgaag ttttgaaaca agctgttcaa gacatcatga aagaaattta tactgggaaa
    10861 gaaacattta aaattatcgc taaaagaagc gatcaccaat ttgaattgac aagtaatgaa
    10921 ttgaatcaaa cattaggtaa tgctgttttt gatattttcc cgcatatcca agttcaaatg
    10981 cgtcaaccag atattccatt acgtgtagag attcggcgtg atggcgctta tctatcgtat
    11041 gaaacgatca agggagctgg aggattgccc gtaggaacaa gcggaaaagg aatgcttatg
    11101 ctttctgggg ggattgattc tcctgtagca gggtactttg ccatgaagcg gggagtggaa
    11161 attgaagctg ttcattttgc tagtccgccg tatacaagtg aacaagcact gcaaaaagca
    11221 aaagatctaa cagccaaact agcaccttat gtgggaacga tccattttat cgaagtgcca
    11281 tttaccgaga ttcaagaaga aatcaaaaag tcagtgccac aaggatattg gatgacaatt
    11341 actcgtcgaa tgatgttgcg tttgaccgat gaaattcgcc gtattcgtca tggtttagtc
    11401 attttgaatg gtgaatcatt aggtcaagtg gcttcacaaa ctttgcaaag tatggtggct
    11461 atcaatgaag taacgaatac gccaatcatt cggcctgtag cgacaatgga taaacttgaa
    11521 atcattgaag tagcagaaca gatcgataca tttaatcttt ctatccagcc ttttgaagat
    11581 tgctgtacga tttttgctcc tccacagcca aaaacacgcc ctcaactgga aaaggtccat
    11641 caatatgaag agcgtttagc aatagatgaa atgatccaac gagcactagc tggcttgaag
    11701 atggaagaga tacaagcaga atcgtctaat gaaaaaactg aagaattttc agaattgcta
    11761 taagaggtta tgaaaatagt gttttgacca gagtattaga gaaacaaaac gaaaaaatag
    11821 ctccatgtat tttaccgttt ttgaactaat gtcgaagatt agctgattga ataccattta
    11881 tcaagaagtt atggaaaagc cggttctttt ttactaactg aaaaataaaa ttttatttag
    11941 agatcaagct acagttgata aatgatataa aaaagagaat ttcccatgaa atagcgaggt
    12001 tgtgacagaa gtgggcagct tcaagaaata agaaggaatt tacgaaaatt gcttttcaaa
    12061 tttttgtgaa atttcggctt atttttcgaa gaagttactt ctggaacacc gtgtatcaga
    12121 ggttg
//
LOCUS       DAYYGO010000086        12009 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000086 DAYYGO010000000
VERSION     DAYYGO010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.088"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1004)
                     /locus_tag="ACHA5T_002041"
     CDS             complement(<1..1004)
                     /locus_tag="ACHA5T_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HFM8887605.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVSNYILD
                     "
     gene            complement(1001..1654)
                     /locus_tag="ACHA5T_002042"
     CDS             complement(1001..1654)
                     /locus_tag="ACHA5T_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8887606.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1942..2875
                     /locus_tag="ACHA5T_002043"
                     /pseudo
     CDS             1942..2875
                     /locus_tag="ACHA5T_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2879..3598)
                     /locus_tag="ACHA5T_002044"
                     /pseudo
     CDS             complement(2879..3598)
                     /locus_tag="ACHA5T_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010745868.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
     gene            complement(3549..3758)
                     /locus_tag="ACHA5T_002045"
     CDS             complement(3549..3758)
                     /locus_tag="ACHA5T_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887607.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            complement(3740..3808)
                     /locus_tag="ACHA5T_002046"
                     /pseudo
     CDS             complement(3740..3808)
                     /locus_tag="ACHA5T_002046"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
     gene            4035..5308
                     /locus_tag="ACHA5T_002047"
                     /pseudo
     CDS             4035..5308
                     /locus_tag="ACHA5T_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(5415..6446)
                     /locus_tag="ACHA5T_002048"
     CDS             complement(5415..6446)
                     /locus_tag="ACHA5T_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8887608.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(6453..7283)
                     /locus_tag="ACHA5T_002049"
     CDS             complement(6453..7283)
                     /locus_tag="ACHA5T_002049"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8887609.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(7280..8095)
                     /locus_tag="ACHA5T_002050"
     CDS             complement(7280..8095)
                     /locus_tag="ACHA5T_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8887610.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(8092..8916)
                     /locus_tag="ACHA5T_002051"
     CDS             complement(8092..8916)
                     /locus_tag="ACHA5T_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HFM8887611.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(8906..10006)
                     /locus_tag="ACHA5T_002052"
     CDS             complement(8906..10006)
                     /locus_tag="ACHA5T_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8887612.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            10184..10969
                     /locus_tag="ACHA5T_002053"
     CDS             10184..10969
                     /locus_tag="ACHA5T_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFM8887613.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            11002..11385
                     /locus_tag="ACHA5T_002054"
     CDS             11002..11385
                     /locus_tag="ACHA5T_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFM8887614.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            11461..11592
                     /locus_tag="ACHA5T_002055"
     CDS             11461..11592
                     /locus_tag="ACHA5T_002055"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8887615.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            11561..11797
                     /locus_tag="ACHA5T_002056"
     CDS             11561..11797
                     /locus_tag="ACHA5T_002056"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8887616.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            11809..11895
                     /locus_tag="ACHA5T_002057"
                     /pseudo
     CDS             11809..11895
                     /locus_tag="ACHA5T_002057"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
     gene            complement(11875..>12009)
                     /locus_tag="ACHA5T_002058"
     CDS             complement(11875..>12009)
                     /locus_tag="ACHA5T_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFM8887617.1"
                     /translation="IELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF
                     "
BASE COUNT         3588 a         2663 c         2139 g         3619 t
ORIGIN      
        1 tcgtctaata tataattact gacagttatt ttcttttgat ttgctaatat ttcatacttt
       61 tttaatatgt cttctaatgt atcatttatt ttcaaatatt cttcatcatt attcaaattt
      121 tctatagaat gaactgataa tatttgagaa atattttttg ttaaactatc aacgatttca
      181 atacattcat taatataaat atctctttct ttatattttc caatattgta tttcatgttt
      241 tccagtatta ttttaagact agcaagaggg gttttaagtt catgggatgc acctttaaaa
      301 aagtcatatt ttaacttttc taattttaaa atttctttat ttttaaattc caaatcgtca
      361 attgttctta ataaagtaga atacaaatca ttaatttgtt gttttaattc tccaacttca
      421 tcattagagc taacttttaa actcattttt ttatcaagtt tcatcatttt atcagtaaca
      481 atttttattt cttgtatatt attttttatt gattttgcat aaattaaaga aataatagca
      541 gaaaataaaa ttgatattaa taaagaatat ggtaaaaatt taaggcttaa atctttcgca
      601 tctttttgca tatcagctgt agaaacaaat tgtagatttg tttttttacc atcattaagc
      661 tttatttctc tttcttcaat tatcaaagaa ttactatcac tttctaaatt aacatttata
      721 ttatctttga tttgtatttc attcttatta tttttttctt ttatgaatac ttttatttca
      781 ctactcttgg aataaaactc caaagtttgt tctatatatt ttatttcttt tccattcata
      841 ttactagaaa tttcatttgc catattatga atcttttctt ttctcgtctc caaatatgtt
      901 ttaggaaaga taaaaaaaat taatgaatgg actaatataa ttattattcc aagaacagaa
      961 aatatttgta tgaacatttt tggaaatatc tttaattttt tcattttctc tccaatttat
     1021 atcctacatt tcttatagta gttatacagt ctaattgtaa tttttttcta agttctttta
     1081 tatatacatc tattactcga tcataaggag tttcttcgct atctttccat acataatcaa
     1141 taatttgcat tcttgttaac acttgtccat tattatccaa taaacatttt aatacttcta
     1201 gttcttttgc atttacatct atttcttcac catttatttt agctgtatag ctattaaaat
     1261 taaccgatag atccttatat tcaaacttct ctaaatgtcc ataattcttc ttaattaaag
     1321 aatctattct agcttttaag accggcaatg aaaatggctt ttctacatat ccgtctacta
     1381 aattagtaaa tgcatcaatt ttatattctt catcactaaa tgcagtcaag attagaattg
     1441 gtaaattgct tttctttcta atttctttca acacttctaa accatttata aaaggtatct
     1501 gaatgtctaa gataaccaaa tttatatcgc tattaaattt tgacaaggct tctcttccat
     1561 ctttagcttg aataacagta tatccaaatt ctgaaagata ttcacttatt ccctctctta
     1621 tcatattatc atcttcaaca gttaatattt tcatatatac ctccacttaa gcctatacac
     1681 tagatattat acagcatatt atggctctat aatatctagt gttggtgacg ccttctgttg
     1741 ccaatacttt ttcttttttt taataaatgc aaacagacac cttgaaatcc tttagaaaaa
     1801 aggcttctat aatattttgt gttgttggaa aatcgaaggc gattttctac taacactttt
     1861 tcttcgtttc aactcaaaaa gagactcact tttggtaaaa tttagtcgac caaaacatca
     1921 ccctaccaaa ggagtcgctc tatggataat catactagaa aattactcgg attaacagat
     1981 aaacatttat tttttgatga agaatggctg attgaaaaag aatataaagg cgttcaagcg
     2041 cagtttatta aagggagatt ggatgactca ccttcccatt gtgaacactg tggatcgatt
     2101 cgtattatcc gcaacggttc ctatacgaca cgtactcaga tgttgaaagt caaagaaaag
     2161 ttgactattt tagaattaaa acgtacccgt tttctatgct atgattgtgg gcagaccttt
     2221 tctgcgaaaa cggacttagt tgatgaacat catcaactaa caaaggaact gaagcaggcg
     2281 attctcatag aactgtacga aaatcaatca cgtaaactaa tcgctaagaa gtacttcgtc
     2341 tcggatggga cggtgacacg gatcttgcgt gaagcaacga agcattacca gccacgtatg
     2401 aacttccttc cgacggtgtt gtgtatggat gagttcaaat caatgaagtc tgtttctagt
     2461 tccatgagtt ttatttgtgt tgatgggaca acccatcagc tatttacgat ccttgaagat
     2521 cgcagacttt acaaactaac tcaatacttc ctgcactttc ctagaaaagc ccgtttaaag
     2581 gtcaagtacc tagaatgcca gctattgtca gctgcttaaa acggtctttc caaatgcgga
     2641 gatcgtaact gatcgctttc atattgtcca acatatcaat cgcagcttca atcaattgcg
     2701 tatccagatc atgaatcgtt ttcgagcctc gcactctgaa aatcagaaga agtaccgtcg
     2761 gctcaagcga tattggaaac tcttactcaa agattccacg acattggagc cgctcaaacg
     2821 gcattatcat cgactgttta aacgaccaat tagtcaaaca gaaatcgtcg atgaaccgtt
     2881 gtgcacaatg gtgtcctgaa cgtacagcga caccttccat atccagagca gtcgccacat
     2941 cacggggatg gatgccatcc aaattaaaag caaccacgcc agtatgatgt tcaggatctt
     3001 gcggtccgta aatggtgata ccgccgatcg cttgtaattt aggtaaaacg taggcaacca
     3061 gttcttgctc ataacgatga atattctcca taccaatctc attcaagtaa tcgattgctc
     3121 ctgcaatgtt cggagtacca gcttcaaatt tccatggcag ctctttccat gaactatcgt
     3181 aaagattgac aaaatctatc atttcaccgc cgaattcgac tggctccatt tgttctaaca
     3241 attgacgttt gccatataat acaccgattc ctgtcggccc gcacatttta tgcccgctaa
     3301 aggcgaaaaa atctgcatcc aacgcttgta catcaactgt catatgcggt gctgcttggg
     3361 caccgactgg actatcctca actacaacag cctgttcaac cctcagccgt ttcaagcaca
     3421 acaaataacc ttctggatct ggtttgccga cctgaagatc atcgatcgtc acaaaggaat
     3481 caattttttc cttaatcatc ggatgtgctt caaaaaaata gtctagctcc cgcagttcag
     3541 aattgagact actccgattt tccagtgctg ctgatccgct gctcaaaatg ttcagcatcc
     3601 actggtctcc caaccgcttg acttatccct tcataaaagg acagttctgc tgccgtccca
     3661 acaccttgtt gatagtctgc aaaaggaatt ttatagccga actgctccag taaataggct
     3721 tcagtaaata gtgatgaata ctctcgtaaa acatccattc actgtcaatc atcgtcccat
     3781 caaaatcaaa aataagtgct ttggtcatct tctttccccc ttacccaaaa caagcaaaag
     3841 ctgtgtcctg ctctctctac agtatcagca taaaaactgc cgtctgaaaa gtcaaaggtg
     3901 aaagaaatgt ggcgctataa tttctaggac tgatagtaaa tcattctatc agtccttttg
     3961 agcataaaaa aacaaaacat acagaagctc cgttagaatt gaatcaccac aacacaatca
     4021 aaggagcgtt ctgtatgcct gatcttcatc ttatcaaact attactagga atcaaagatg
     4081 gaaacattca aatcactcat gtcgagaaga aaaaaataca ttcaatcaac agtatcgttg
     4141 tttctgccac gctcattaaa acgattcagc cctgtcctca gtgtaaacag gtgaatcaac
     4201 atgggctgat cgttaagaac ggaaagaaaa aatcgctggt ccttctcaat aagtgtgcga
     4261 atcaacggac ttatcttgct ttggcaaagc aacggtacct ctgtcgtggc tgtcacacct
     4321 attttacggc gaatacctac attgttgaaa aaaactgttt tatcgccaaa caagtacatt
     4381 ataaaatatt ggaggaattg actgaaaaac aagcaatgac gacgatttct aaacattgcg
     4441 gcgtttcttg gtcaaccgtt tcgcgaacac tcgcttctct gctccccatg acaaacgtga
     4501 aaataaattg gttgcctcgc tgttcattgg tagatgaatt tcgttcattg aaaaatcaag
     4561 tcggtcccta ttctttttct tgtatggatg gcgatactgg gaaattactg gatattcttc
     4621 cttcacgtaa gaagaaagat ctcatttctt attttatgca atttgaacga cgtgcacgtt
     4681 taaacgtaaa aatactcgta acagatatga acgcctctta tgctttgctg ataaaagagt
     4741 gctttcctca tgccaaactc gttgtggatc gctttcacat cgtcaagcac ctcattcgca
     4801 gttttaagga tatttgacta tgagtcatga aaagcttcga ccgaaacgat ccaatacaag
     4861 ccaaacacta tcgacaagtg aaggctttat ctcgacttct gatcaaacga caggacacgc
     4921 ttgtttatga taaatggaca aaatggcgta attttggttg ggcctattta accgaaagtg
     4981 aagtcgtaga acgtctactt tctacttctg atgaactacg aatagcttat gcttactatc
     5041 aagaaattct tcaagctttt tatgacaaag aagcggacat cttctttcag cttgtaaaaa
     5101 caatgcccaa gtcggttcca agagaactgc atcatataaa aaaagcgttt ataaactatg
     5161 aatccggcat tcgtttagcc ttagagttgc cttactcaaa tggcaaaata gaaaatctgc
     5221 atacccacat caaagcattg aaacgaatag cttacgggtt aggatgagaa ccagaatttt
     5281 cttaatcaat catctgatca cttattaaac ataaaaaaac atctaaagca gaaaatcaat
     5341 tctaccttag atgttcttat atcactctat cagtcctatt tgacaaagag ccagaaatgt
     5401 ttgccgacta tacactagct ttgttgtgca aaatgctgag tatcaatttc cacagggatt
     5461 gctagaatat ctttcagctg aatgtataag gcatcgctaa ttttctcttc ccgatcgttc
     5521 aaccgatcca ctaaaatttc tttgccctcc agcgtttgaa aaacataccg ttgaatgttg
     5581 cctaaaataa atgattcctt ccatagaacc ggaatcttaa ccccctgagg aaccggttca
     5641 aattgaatca gctccggtct taaataataa atgcagcgat tgcccttagc ggaaaactcg
     5701 tgggtatact gattcagctc ataccattcg aaacgattat agttgccgat aaattccgca
     5761 acaaatcggc tctgaggatt ctgatacaat tccttcgagg tggaaacctg agccacccgc
     5821 ccagcttcca tcacaaaaat ccgatcactg attcgcatcg cctcttcttg gtcatgagtc
     5881 acaaaaatca tcgtcatccc tagcgattgc tggatagcgc gcaaatcccg ctgcagctgt
     5941 tttctaattc gggcatccaa agcacttaat ggctcatcca gcagcaggac tttcggttca
     6001 gtgaccaatg agcgagccag tgccacccgt tgtcgttgac cgccagaaag aaactggaca
     6061 tttttatctg cctgctccgt catatccacc aatgatagca tctcaaaaac cttttcctga
     6121 atcacctcag gactcagctt ctttaccttc aaaccaaagg ctacattttc aaaaacagtc
     6181 atagttggaa acaatgcata ctgttgaaaa acaaagccga tattgcgatc cttcgtaggc
     6241 ttatcggcaa tattctcatt gtccagcaca atctcaccgc tctttggttg ttccaatccc
     6301 gtgatacagc gcagcaacgt agactttccg cacccgctgg gacctagtaa agtaaccagc
     6361 tcaccctttt caatctgaat ttcatcaata gctaaaatct gtttttcatc aaaaaacaga
     6421 ttgagatcat gtatttctaa aaacattttc cgtcacaact cctttaaggg cttctttttc
     6481 gactgcttct cttccctcgt ctgcttaagg gacagctgtt gcttttgtcg ataagtcagc
     6541 ataaaggtag ttaaagcaat tcccaccata aacagcaaat acaaaaccat cacagcgcta
     6601 gccaaatggc cgttttcgtt catccgccgc agcatataga tgcgtaccgt ctgaaatcga
     6661 ccgccaatca acaggttcgt cagcatgtac tccccaaaaa ccgaagaaaa aatcatcaga
     6721 gaaactaggg tcacaccgat acggatgttt ggaaacagca cctggataat aatcgtagac
     6781 atcggggcgc ccaaggtttc tgctgcatcc actaattcct tcagattaat taagcgcatc
     6841 tggttgttaa gactaagata gacaatcggc aacgccgtaa taaacaatgc acctaccaac
     6901 actacaaaca ttgggatacc agtttcagca tacgttcgaa gcaaagccgt taccaaaatc
     6961 actcctggta aagcatacgg cagcaggagc agcttttcca gccaacgatt caaacgaggg
     7021 aaatacaaat gaatccaaat aatggtggga atcatcaaca acagaatcgt caccaagacc
     7081 acacctgcta ataaaagtga gcgacccacc gcagcaataa attccctgtc cgtcagcagc
     7141 tgttggaacc attgcaaggt aaaatcacta ggtaaaagcg attttgacca tttagtggag
     7201 ctggcataca aaatcgttcc aataatcggc agcaagtaat acaggacaaa gcagaacaaa
     7261 atcggtttta ttaacttttt cacgtctgca ccaccttgcc tttcttttga ataatcaaca
     7321 ttgccgttac cagcaggaca cctaaagcga ctgccaacgc actgcctaaa ttaggcttgg
     7381 cgaacacatc tcccgacact aaagccgcaa ttcgaatcgt taaaagattt acattactgc
     7441 tggtcagggc atacgctgtt tcataggtcc ccatgccatt agcaaataag atgacaaagg
     7501 tgctggcaat cgatggtcgc aaaaaaggca aagccacctt gaaccaagca tacgctgatg
     7561 aagcgcccag caattgtgcg gactccagcc attccttttt cacttccttc atgctgggat
     7621 acaaaaacaa gacccctaac ggaatttgga aataggtata cgtgatggtc aatccccacc
     7681 aagaatacaa attgaagtcc gctagaaatg gcaaaatcat tttcagcaca cctacattcc
     7741 ccaacaaaat aataaaagag aatgccagcg gaatcccagc aaaattagct gccaaattgg
     7801 aaacaaaggt gattttctcc tgcaatttag tcgatagatt cattaagcat aaacataaaa
     7861 taaacgtccc gattaaccca atcacactgg agctgacagc aatcagtacg ctgttaacaa
     7921 aggcctgtgt gtaatatttg ttgctgaaaa tatcactgta attggccaat gtccactggt
     7981 tagttaaatc actgtgcaag ctgcacagaa ccataaaaag cagcggcagc agtaaaaatg
     8041 cacagaagat aaaggaggca ggaaccagaa ccaagcgttt tttcatctct ttcatcccct
     8101 catcaattct tgaatgtcct gcattttctc tgacggtgca attcctaata accagcaaaa
     8161 caatggagcc gccagcagct gtggtacagt ttgttccttc agcttttggc gtttatttga
     8221 aatcaaataa aaaggaactt cacgatgtgc agccaagctg ccgccatgca gtccgtgcgc
     8281 atccatcccg tgatccgcag tcaccacgac ttgataacct ttttgcagcc attcaggaag
     8341 gtaaatagcc aataatctgt ccgcctgatt aaccgctgct gcatattcat gagaatcggc
     8401 ggtaaatttg tgaccgacat catcaatatt catcgaatgt acgaccaaaa agtctggctg
     8461 atactgattc actaaataat gggcatccgc aaataaatgc gaatcagggt aagtgtcctc
     8521 actataaaag atgccatgct gaatcagtcg ctcctcctga tgctggatgc gatcaaccaa
     8581 acgattatag ggggcttgat tgtacaactc gctgtaccag tggtaagcgg cggctcctgt
     8641 ttttccgcca gcttccttaa ctaattcaaa cagcgaccac tctttggatc gctgagcatg
     8701 gtaattacta tagatgccat tttcataggc tggaacgccg gtcatcagta cttcgtataa
     8761 cggacgtgaa ttgctgggca gctcactttt taccttcaat aagcttgctt gctggtgctc
     8821 aactaaatga ttcatgaacc caagctgggc gacggctgtg tcgtagcgac agccgtctag
     8881 tataactaca atcagtttgt caagtctact ttgcataggt cagtacctcc gcctgccaaa
     8941 gctctggaag ctctacagag gtcttgttcc aagcttcatt gtcttcaact ggcaccgccg
     9001 cttcataagc atcctccggc agtaattttt cagctacttc tttaggcagc tctaccttgc
     9061 gaataggtcg cgcatagccc tttgccaaat tgatttgccc ttcatcagat aaaatatatt
     9121 cccgtgctag ctttgccgca ttgggatgag gggcattttt attgattaag gttgtatacc
     9181 cgctaatgat ggagccgtct gttggcacag ttacctcaaa acgctcggag tcaatttggt
     9241 cacgataatt tagtccattg aagtcccaaa caactgccac atcgatttca cctttttcaa
     9301 gatttgctac gctggcatca acggtggaca aacggccttt ttctgccatt tttttgtagt
     9361 aattcagacc tggctgaata tccttttcgt cacctccgtt ggcaatcgcc gcagccaata
     9421 cagcaaattg tgcttggttg gcaatcgtca catcccccac gctaacttga tagtcaccct
     9481 cttgcagctc cttccagcta gtcggtgctt gcttcacatt ctctttatcg gtgataaagg
     9541 aaatggttcc cgtatagctt aacaaccaat ccccatcatc atcctttgcc caatccggaa
     9601 tttcctccca atagttggtc ttataaggta aggttaactc tttgctttca gccaacggac
     9661 cgaagctgat accgacatca ccaatatcgg cggttccatt ttttccttct gattcaaact
     9721 tagccaattc ttctgcactg gacatatccg tatccacgtg acttaagccg tattcttttt
     9781 caaggtcgtt ccaagtgcct acccaatttg cccaagtatc cggcattccc aagctggcaa
     9841 tctctccttc ttccttggct ttttctataa tcgtatctaa agagtcgtct gccgcagccg
     9901 attgcttcgt tgcttcacct gagccgcagc ctgtcagcag gatacttact gttgctgcac
     9961 aaaataaacc gatagtcatg ttacgaattt tttcattcat tttcattcat tcttctcccc
    10021 tttcgataat tccattctaa caaagctttg taatgagaga atcagtttct tgaaaaggtt
    10081 tcatgaacaa tgtaaaggtt gtatatggtt tgtaaatttc gacgaattcc cgttaacctt
    10141 ctaaagcttt tgcggtatga tagtagaaaa aaggaggctc cctatgcagt attatgatca
    10201 gcatctccac acgtattttt cctttgattc agaggaaaaa tttgaaaact atttagccta
    10261 tcagccggag tttttcgttt ctacggatca ctttgattta aacaatccat ctaccggaaa
    10321 agatgacatt ccggattatc cagcctatat aaacaaactg gactctttaa aagaaaccta
    10381 ttccaccaaa tttctgcgag gtatcgagat tggcgttgtt ccaggacaag aagagcggat
    10441 cgtggattac ctcgctcagc atccttatga tttaaagctg gtaagtattc atcaaaatgg
    10501 ccgctttgat tatatggacg atgtggtttt aaccaaagat cccattcaaa ctaccaagga
    10561 atattttgat cagatgttgc aggtcttgac ggattttcca gaaggagata ttttgactca
    10621 ctttgattat ggattacgcc gcttcacctt cacagcggaa gaattcgcgg agcattttga
    10681 ggctcagctg ctaacgattt tcaaaaaggt tgttgaacga gaattggcga tggagttaaa
    10741 tgccaagagc ttcttgaaat acggcaacga agacctttac cgttatgccg ttcctttgta
    10801 tcaaagtgtc ggcggaaagc tctttacctt aggctcggat gctcatgttg ccgaagatta
    10861 tcagctgggc tttaaaaaaa tgagtcagct gttggcagac tgtcaggtag aaaagttgct
    10921 agtcattcaa ggaaaagaac gccgcctgac gccgctgccg attttgtgat tcctatataa
    10981 tagaagaaag tggtgaaaaa aatgagcaac ctgtgggact acaatcaaga ggcacccatc
    11041 cattatttaa ttgcgcgaca ttgggatgcg ttgaaaattg aggcggtttg ccgatccttg
    11101 ttagcagccg ttccaaaaca acaactggaa aattttttgg tggctgacag cttgcagcgg
    11161 gaaaaagttc aggcttattt tgcagccttt aaagatcagc ctttggaata tttgcatgcg
    11221 cagtttcatc ttttttatca agtagccgcc ccagatgact acaacgattt acgaggacag
    11281 ctgcaattga cctttcaagc ggatgaaacc gcctataccg ttttattggg aatggctcgc
    11341 cttggtgatc aggcgaaggt cgagtggcgg attttcgata tttgatggca ggatcaaggc
    11401 gtttcagttg caaaccttcc tctctttcaa ttagaaaaac cgcagcagga agaagtttgt
    11461 gtgctggtca ccgatgctgg tgaaattcgt ctccgcttat ttcctgacca agctcccctg
    11521 gctgtagcca actggcgctc cctagctgag caaggcgttt atgacaacac acctttcggc
    11581 tcgtgtcatt aaggaatttg ttattcaagg tggcgctttg gatggttctg gtgatgaagc
    11641 tgtttccagc tatgagggct ttttcgctga tgaagtgcat cgcggacttt atcattttaa
    11701 tggcgccttg gccttagggg accacggccc ccacaccaac ggcaaccagt tttttattgt
    11761 gcaaaacaca aaggcgcagg ctgacctgct aatgtaaagc aagcatactt ggagcttgga
    11821 ggactaccgg agctggatgg tcgttatact gtttttggac aggtatacca agggttagaa
    11881 atcgtagcag cgatcgcagc tcagccgaca aacagtgaag atgtaccgat tgagccagtg
    11941 ttcattcgac aaattaactt cgaaaaaaca tcgtaaaaag acactcactg acttcagcaa
    12001 aagctctat
//
LOCUS       DAYYGO010000087        11923 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000087 DAYYGO010000000
VERSION     DAYYGO010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.089"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(367..1158)
                     /gene="msrA"
                     /locus_tag="ACHA5T_002059"
     CDS             complement(367..1158)
                     /gene="msrA"
                     /locus_tag="ACHA5T_002059"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM8887618.1"
                     /translation="MLDVEQMEIDLYNIILNRNTREWERTQFEEAKEDLAKMSIKQVI
                     EKLEVNLRPLALRDNLTPDAMDFYLRITGESASAKAYDYAKHKITSPLYQERAIFAGG
                     CFWCMVEPFETKPGVISVLSGYTGGETKKPNYDQVSGGYTGHVEAVEIIFDTRQITYE
                     ELLEIYWQISDPTDAFGQFQDRGKQYRSILFVVNEKQRQLAEKSREQLIQSNRFSRPI
                     VTEIRQAKTFWPAENHHQQFYQKQPKRYKKIKHSHQLHLFFQRHR"
     gene            complement(1349..1984)
                     /locus_tag="ACHA5T_002060"
     CDS             complement(1349..1984)
                     /locus_tag="ACHA5T_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFM8887619.1"
                     /translation="MIFLQKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFIGTSKTLAILAVATVGLYGIGDCIFT
                     GLFSINTNESSWNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(2250..3302)
                     /locus_tag="ACHA5T_002061"
     CDS             complement(2250..3302)
                     /locus_tag="ACHA5T_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8887620.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(3315..4253)
                     /locus_tag="ACHA5T_002062"
     CDS             complement(3315..4253)
                     /locus_tag="ACHA5T_002062"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFM8887621.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(4704..5354)
                     /locus_tag="ACHA5T_002063"
     CDS             complement(4704..5354)
                     /locus_tag="ACHA5T_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887622.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(5454..5963)
                     /locus_tag="ACHA5T_002064"
     CDS             complement(5454..5963)
                     /locus_tag="ACHA5T_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFM8887623.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(6164..7033)
                     /gene="scrK"
                     /locus_tag="ACHA5T_002065"
     CDS             complement(6164..7033)
                     /gene="scrK"
                     /locus_tag="ACHA5T_002065"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFM8887624.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(7053..8174)
                     /locus_tag="ACHA5T_002066"
     CDS             complement(7053..8174)
                     /locus_tag="ACHA5T_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFM8887625.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(8176..8571)
                     /locus_tag="ACHA5T_002067"
     CDS             complement(8176..8571)
                     /locus_tag="ACHA5T_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8887626.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(8590..9387)
                     /locus_tag="ACHA5T_002068"
     CDS             complement(8590..9387)
                     /locus_tag="ACHA5T_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8887627.1"
                     /translation="MSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPIL
                     RKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTM
                     GPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYK
                     QGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLD
                     KILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(9389..10177)
                     /locus_tag="ACHA5T_002069"
     CDS             complement(9389..10177)
                     /locus_tag="ACHA5T_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8887628.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(10191..10667)
                     /locus_tag="ACHA5T_002070"
     CDS             complement(10191..10667)
                     /locus_tag="ACHA5T_002070"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8887629.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(10739..>11923)
                     /locus_tag="ACHA5T_002071"
     CDS             complement(10739..>11923)
                     /locus_tag="ACHA5T_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8887630.1"
                     /translation="REVQLVNRFIQIGDIYLTKIQKNVLVILFKLMCSKEFQQKRLAL
                     ILAHGDLTATCIASTANRIVERYIYDAIDMPLMTSSSEIADKINRYLQECTGYEEIIL
                     LVDMGSLEKIYLNLELDAQKKFGLLNNVSTRMAIDVANQILLDLPLKQILEQVKENQN
                     VDYIYESSNIKKDMIVCSCASGLGTSFKLKKILEDSFPRDTDLVIETCDYYSLVNENY
                     LSELRKENNILFIVGTLNPHLEDVEFFSIEDMILGMNISKFSNQLNRHFNDNELKVLN
                     NNILKNFSLTNLMEQLTILNPTKLLERVSDAIYILQNDLGISFDNNTCFGLYVHISCL
                     IERLVKQNTLEDEIYFNETSEEFQKFQTHFKQSFSVVEHYYSVDIPIHEVKYVYDYVK
                     RA"
BASE COUNT         3780 a         2511 c         1728 g         3904 t
ORIGIN      
        1 tatagttatt gtttgcgctt ttatacggaa tttagttcat cattaatgtg gaattcgatt
       61 aggtgtctat taattatcaa gatgattctc tacaaaattc catagaaaaa tcaagattct
      121 acaattcgaa ttctctttca catggaaata aaggctagta aaaaaacagg agttgtgaca
      181 aaagacttgt catacctcct gtttccttgc gacttgaagc tgaatgcttt gcgccacaac
      241 cttttcctcg gtgttctcaa agcaactcct cgaaataatt cataactgtc caaaaatatg
      301 aaaagctatt tttgtcagtt gctcattatt ttgtcggagc tgaacgcttt gcgccacaac
      361 cttcctctat ctatgtcgct gaaaaaataa atgaagctga tgcgaatgct tgatcttttt
      421 atatctttta ggttgcttct gataaaattg ttgatgatga ttttcagctg gccaaaaagt
      481 ctttgcttgg cgaatctctg taacgatagg acggctaaat cggttagatt gaatcagttg
      541 ttctcttgat ttttcagcca attgcctttg cttttcatta acaacaaata gaattgaacg
      601 atattgtttc ccccgatctt gaaattgacc aaaggcatct gtagggtctg aaatttgcca
      661 ataaatctcc aacagctctt cataggttat ttgacgtgta tcaaatatga tctcaacagc
      721 ttcaacatgc ccagtatatc caccactgac ttgatcgtaa tttggttttt tggtctctcc
      781 acctgtataa ccagataaca ctgagattac acctggtttt gtttcaaaag gctcaaccat
      841 acaccagaaa cagccaccgg caaaaatcgc ccgttcttgg tataagggtg aagttatctt
      901 atgtttggca taatcataag cctttgcact cgctgactcc cctgtgattc ttaaataaaa
      961 atccatcgca tctggtgtca aattatctcg tagtgctagc ggacgcaagt tgacctctag
     1021 tttttctatc acctgcttta tcgacatttt tgctaggtct tcttttgcct cttcaaattg
     1081 tgttctttcc cattctcttg tatttcgatt taaaataata ttataaaggt ctatttccat
     1141 ctgctccaca tctaacatga agtcaccctc tttcaagttt tcttcttatt cattcaaaaa
     1201 atgactacct gaaatttagc acaacgcata acaagttaag actaacttgc ttttcgctct
     1261 cctcatctct attcttagtc gaacacatcc acacgaatgg ttacccatag agaaattctt
     1321 ttacttaatc agcttcacac tgctttttct actttcgtag ttgaaaaaaa cttaaacaaa
     1381 tcatcggcaa atagttaaag aaaaaactta agcgttgaaa gaagcccaat tgcttaaaga
     1441 aggcaaactg actaatcgac ggaatccgtg ctaatccgta aagacctgca attagtaaac
     1501 taataactgt taaaacaaga taaaaatggc taagcgtacc ttgaccactt tgacgataaa
     1561 gcagaacaag taacaatggg aaaagtagaa agcctgcata gcctagccca gaacctatat
     1621 tatggatcca agtcgataga ttccaagagc tttcattcgt attgatactg aataacccag
     1681 taaaaataca atcacctatc ccatataaac caactgttgc aacagcaagt attgcaagtg
     1741 tttttgatgt tccaataaat aggtgataca acgcaggcaa cgaaagaaca aaaaaaagtc
     1801 cagatacaac tgaccagacc aagaaagcac gtctgactgg actatctacg tcaccaaaca
     1861 cactaattac ggtagtcatt tgattcagtt tgggataaaa aaggccaagg atatacggtg
     1921 tcgataaatc actaatcaca cctaataata aaaaataaaa accatatttt tgcaaaaaaa
     1981 tcattttctt ctcctacttt ttagtgatta ttcgatacat ccaacaatca aattttttct
     2041 cattttaaca catattttta ttctagttgc cttttgaaca atcaataaca taaaggtgag
     2101 tttcttctac attttaaaaa atcgaatggc actgaagagt tcagttgtca gcatcaatct
     2161 cctactcaaa aaggcaaaaa aacgtaagtc agatgttgtt gacatctcac ttacgcatcc
     2221 attgattgaa aatttcttag taaaatctac tagcctaaaa atttgaagac ttcttctcga
     2281 tcatacagtt taaatacttt ctcgcataaa aattgagtaa ttagtgccgc aaaaacaggg
     2341 atgactaacc aacaaagaac aacaagaaag ccatttagcc ctgcatctaa agaagccaac
     2401 ggaccaacaa gtccgaccac gccgaaacca gcagaagcag gcgtacctga aatagaaaat
     2461 agagcgacag gaatagctgt aatcactgca ctaactaaac aaggaataag aatcacaggt
     2521 tttctaaata gattaggcat catcatcttc atagcgccta aagcaatcgc aattgtcaca
     2581 cctgacttat taaccttcca agagttcact actaaaacaa aagtggttgc agctacgccc
     2641 atcgcagccg caccagcaga aacaccatct agttggattg ccattccgat tgctactgtt
     2701 gaaataggag agataatcaa aaaggcaaag gaacaaccaa tcaagataga cattaacagc
     2761 ggttgtaagg tagtgaagtt attaatcaat gatccaattg cagaagtaat tgctgttaca
     2821 aaagggaata aataaaaacc aagtaaacct gcacctacac ctacaacaat cggtgttaac
     2881 acaatcgaca ctgacccaaa actatttcca atcagtaaaa tcagcccgac agcaatcgat
     2941 gctgtcagca tcgtattaat aatatcgcct gtaccagcac caacgtaaac acccgtttca
     3001 cccaaatcag gtgtgaattt gactacacca gaaccaacaa aagaagctcc ggctacaacc
     3061 atcattctca tcggatcaaa tccaaattgt aaggcaatca aaccaccaat gatcaaaggt
     3121 gttgctagtt gaaaaataac tgctaactgt gcgatcgtta ccgcaaaggg atacgcacta
     3181 aaatatttta aaatggcacc taatactgca ttaggaatca atccaataat gattcctgtt
     3241 gctgtaccag cgaggacttt attcagaaaa attttcggtg atattttttg tgaagtttcc
     3301 attcaatctc tcctttattt tacgttcaaa ctatcttctt ttgcatggac aagctcagtc
     3361 aagaaatcac aataaggcgt cgcaacacca tatttcttcc cttttctaga aattgcgcca
     3421 ttgatataat cgatttctgt taatcgatgg ttcttaatca agtcttgata cattgaagga
     3481 taatgtaacc caattgtttc cgggtcaaaa caagattcac aatgtgcaat gacttctggg
     3541 acatctagtt caatcttctc tactgctgct actttggcaa actcattgac aatcgtcgcc
     3601 accattttat gagcagtcga tgtttttcct agctcagcca tattaacgtc taagattgtg
     3661 catagtccgt tcattgttcc gttcacacat gctttgcgat aaatagaata gtgaatattg
     3721 tcagaaaaat gagcatttaa tcctgattca gacaatttgt ctgctaattt ttttgcagct
     3781 gcttcttttc catcacctaa attttgtaat tctactgaac cacttccaaa taatttgacc
     3841 tgaccaggac cttcaagacc tgccgtccac atggtatttc caatatagat attttccatt
     3901 ggtacaaatt tctcaataat atcttcatgc ccgataccat ttaatagaca taagacctct
     3961 gtatcttttt tgattaatga ttggatatcc tgcagcattt tttccagctg catcgctttg
     4021 gtaaatagaa taatcagatc aacttgatct tctttttcta cttcggattg aagaacgatt
     4081 ggtagttttg cttctacctc tttaccatta aaatttgctt gtaatccatg ctctttgatt
     4141 tgttggacat gttctgccca gccatcgatt aataatactt cgttcccact ttggtgaagc
     4201 attaacccaa agcgacttcc cattgcgcca gcgcctgcaa ttgctatttt cattgattct
     4261 tcctcctttt tctttctatt atattcattt gagaatcatt tcacaacctt gttttgttat
     4321 tcttctctta tttatcatgt ttatatccac cattcaagtt attttatcag caaatcaata
     4381 aaaactggaa tagaatccca atttcatcgt aagagaaact agactagcaa taagaaaata
     4441 gaaacattca tagaaaaaac ctcactcctc tgattataaa agagggggca ttaacctcgt
     4501 gtatactaaa accttccatt cattcgatgg atgattttct ttttgtgaaa aaatattcat
     4561 tgtagtacga tgttgcgcaa cacagacgga tggtattgct tattatttat taaaatcgaa
     4621 caatacactt atttaaaaac ttctttccca gattttcttc catttttaca gttataacta
     4681 gtattatcat gaaaaatagc cttttattgt ctatgggaat aataatttaa aattggtact
     4741 tttttattaa ttagttcatc gcccaataat ctctccagtt tcttagcatc tcctgactca
     4801 accggaatta tccaagccca gtccaaataa ttttttaaaa tctcatcata ttttgcaaat
     4861 atttcattgc catattccgt ccttaaaatt atattttcag tgccagacaa gtgatttgta
     4921 ttgagttgat aaaaaagatc tccatctaaa aaaatatcat tagaagaaga tttccccaca
     4981 gtaatgacca ttccatcagc tctatgtgtt acatacaaat aattgtatga cctcatttca
     5041 cctaatgaat caattatttc ttgcccttta gctctaatat attgatctct attacctatc
     5101 tcactcttat ttaacaaaag ttttttcatg tcaaatttaa tcggctgttt tatttttgta
     5161 aattcgcttc tactactaat ccaatccgag attaattttt caacatgtcc cagagtaaac
     5221 tctgatttat tcgctaaata ctttttttgt tgtattaaag acatctttcc ccgaagctca
     5281 aaattgagcc tacatcgatt ctctatctct tcaaaaagca tcttatcttc atcattctta
     5341 tattcatcat tcataaaacc aatccagctc ctcgattaag ataatatttt aatctcatac
     5401 aaaaactagc tctcatcaaa acaaccattg ttttgatttc tcatgaaatt tctttatact
     5461 tttctccgat tataaattcc taaccccttg agaaaattac gcatatattt atctccgcaa
     5521 ggcttaaaat ttcgttggcc ttcttttctc aaaaatgccc cgatttcgcc ttttgatgga
     5581 tagatttctg cctcagcaaa aacgtctaaa atatcatcag tggtcataga caaggcgatt
     5641 tttaattttt ttagcaacac attgttgata tatttgattt ccttgctttt ttgcggcgtg
     5701 tctactatcg gcttatcttt ttgcggacca cgcgccaaga gaatcaggcc atttaggaat
     5761 gcgtcaaaaa cttggttgtt gatggttttt tcatacacat tattttctgc attatctttg
     5821 ttcttatcct gtactttggt cagtaccgcc tgagcttctt ctttagtcag tggtacaccg
     5881 cctagctcaa aagctttaat caaatccgta tctttcaaat caatcgcata acgcaatcgc
     5941 aataacctat cattgttatt catttgttcc tccgaagtat ttagtcttta ctttcttacg
     6001 tactttttgt cttgctaaga tttttaggat caatgataaa aaccgcatca cttatgataa
     6061 ggcgctgggt atcatgagtg aatgatattg ctgaatacaa atagtccgac cacattaagt
     6121 tgatcggact tcataagcat tttattgact aatacttttt tgcttaagaa ttgattctaa
     6181 catcttttgc taaagttaag catcctatag ttcctgcatt atctgctaac ttaggtgtga
     6241 caatgtattc ttcgatattt ggcgtatcga catagccgtt aattagttct aaaaaacgtc
     6301 tttgtacttt ctttttcaga tgttcttgtt gcatgacacc accaccaaaa attataatat
     6361 ctggtgaaag catcagggtc gtattacagg cacattgtgc aatataatcc gcctcgattt
     6421 cccagaacgg atcttctata gaaagatctt gtcccttggt tcccgtacgc atctcgatag
     6481 cgggtccaga tgccaaccct tcgagacaat catgatgaaa aggacaagta ccagagaaag
     6541 tatctcctga atgtctctta actaacgtat gccccatttc tggatggcta aacccttcaa
     6601 caaaaatccc atcttgtata gctcctccac caattccagt tccaacagtg taataaacaa
     6661 cactggataa accttttcca tgacctgaaa catactcccc gtaacaagca gcattcacat
     6721 cggttgtcca ggcaattgga atttcgaaat gcttattcaa cgttccaaca aaatcaaaat
     6781 ttttccaggc agtttttgga gtagaggtga tatgtccata ggtctttgat tcacgatgga
     6841 tatcaatcgg tccaaatgac cctactccaa tgctttctaa ttgttcttta tagtgattaa
     6901 agaaatcaat taccaaactc attgtttcat tcggtgtagt tgtaggaaaa ctcactcttt
     6961 ctaaaacctt taaatcatca ttgccaatgg cacaaacaaa tttagtccca cctgcttcta
     7021 ttgagccata catttcttac tccaccgtcc ttttatctag gaattcgttc ttccctgtct
     7081 aatagttttt cgaaaaactc atgtggttcc ttttggtacc aataggcaac agaagaaata
     7141 tcgtcttgac gctcataaag cttgatgtca tcatttccta gggtttggaa agtaacacgt
     7201 aaattttctt tgaaactaat ggggtcgggt aaatgccagc ggtacaaccc atgcatcggt
     7261 aaagcatcat cgccaaaacc atgaactgga tttggattag gttttccagc actaaaataa
     7321 tcccgggtgt gatctctttt agattgaaaa ggatagccca taaatagcgt attaaaattt
     7381 tgagcatatg ccctttctcc aaagttcttt ttatggaaag cccaagcgcc gccaaaataa
     7441 tcttctgctc ctgtagatgt tattgtaggg aaatcttcat ctccatctat ataaaactta
     7501 aattctcctt ccccccacca gtatctttcc aatgcgcaca aagcaaagta ggttcctaca
     7561 taatacccag tgcctttgat tccatcaata agggtgtaat cttttccttt tgtcgtgatg
     7621 ggttcacggt tccatttagc atgaaaatac atcgcatcat ccggtacttc agtcaatgca
     7681 taattaaatg tataaaaaat gtgcttaata aactcaggat gttcattcgt taaagtaatc
     7741 ttagcttttt ttcgaaatgg catctcaaaa tagcaattca tccctgatgt tggattcact
     7801 acgattggta aggaattcac ttcgcatctt gtcccaaacc cattacaaaa aaaatctcct
     7861 aatggacttt ctacagaagg agtggattca tcatcccaat aaatacgcaa aattaaatca
     7921 cgtaagacga aactcccagc gattgttttg tctgtcactg tcatccaaaa atgacgtaaa
     7981 attccagggc cttccatttc tgctaaagta attgtctctc cagcatttat cggaacagaa
     8041 ggtgaccctt ttctcccaat tcctagatga ctcgctgtca ttgccgcttt ccctttttca
     8101 ccatttttat tttctggtga aatagttcta ctttcctctc ccttgtggag ccatatatta
     8161 tttaaacaat tcattttatt cgtcctctat cctttcttca cttttttgat tattcatata
     8221 gatgatttgt tgttgtgcct ccgcaataat tgctccaata tctaaattat catttcctaa
     8281 ctgcaaagcg attgacattg ccaagggaag attcattcct gcaattacgt gcactctgtc
     8341 agaaatatag gtaaaaaact tttgatttac acttcctcca gccatgtcag ttaaaacaat
     8401 tatttcgtct gtagtatcca ctaactcttt aatttgttca tctatatcat caatttcttc
     8461 gacatatgca cataagctac taatttgatt atgcatacct gttaaaaact ctagtgtctc
     8521 tttaaaaccc tcagctagta ttgaatggct agcaatgatc aatcttctca taattcaaat
     8581 ccttttcctt tacgctaata ttccaaatgc agccataacc atcgcaaaaa caataacgag
     8641 tagaatcaaa atggtcatac tgattttttt cttactgagt agataatatg ctgcagcagt
     8701 aataattact ggaattaatg ttggtaaaat tttgtccaac attggttgga ttttcatact
     8761 aacttcacca aaagtgaatt taagtggcgt tgaaacactg ataactgaag caattaatga
     8821 tccaactact gttagcccca atactgaagc agcttcagtt aaataattaa tcttttcacc
     8881 gtattcagta acaatcttca ttccctgctt ataaccaaat tcaaagcctc tcattcggaa
     8941 aaagaagatt accatgatta aaatgaacca taaaaagatt cctacaggat tattttctaa
     9001 agccatataa gccgcaattg atcccatgat ggttgggatc atcgtcatca aaagagagtc
     9061 cccaatacca gcaattggac ccatcgtacc aactttaaag tcattaatcg catcttttgc
     9121 ttctaatcct tgcttttcct ccattgcaac acaagcacct aaaataatgg ctgccagcca
     9181 aggctgtgta ttgtaatact gaaagtgatt atttaaagct tcgatgtatt catcatcatc
     9241 tttgtataat tttcttaaaa taggtgacaa tgcgtaagtc acagatgcac cttgctgtgt
     9301 ttgataatta aaggtacaaa ctgacatcaa ccaacgatag ttcgctttcc taagatcctt
     9361 tttcgttact tgatattttt cgctcatttt attcatcctc cccaataaac ccaacatcag
     9421 aagctcccgc agaagcagtg atttgtgcat ctttttcagc tctatgatac acattaaatg
     9481 ctaaagcgaa tcctatgatc gccgctccaa taataggtac cttcaaaaaa gcggctaaga
     9541 aaaatccaac aagtaaataa ctaatgtatc gacctgtagg cataaagcgt aacaacatcg
     9601 caataccaac agcaggcaac atttttccgg ctaatgttag cccatccata aaccaattag
     9661 gtaaaacatc caaaacgtaa ttaacagcag actgtcctaa agtaacggca ataaatactg
     9721 gaattgctgc agataaacca aagaaaattg gccccaacca caaaacacta ttcattttct
     9781 taaattctcg tttattcgca tattcctgtg atttgcgaac aacaaaacca tttaagattt
     9841 tgacaattac atctagttgc acccctaaca tacctacagg tagtcctatt gctaatccgg
     9901 cttctactcc aagctggcta ccaaatgccg ttgcaataat tgcgcacgtt ccatagtccg
     9961 gtacagatga gccaccaaaa ccggcaactc ctaatgacat caattgaact gtaccgccaa
    10021 ctatcaaacc tgtttttata tcacccatca aaattcctgt gaataatccc caaaaaactg
    10081 cgtaatttac aaagatcatc ggaatcccat aataatcaac atgtttaata aatgctaata
    10141 tagtcagcat aataatttga aaaatagtaa aatgcattat ccttactcct ttatcctaat
    10201 tttgattgaa tttcctgtgc cttatccgcc ggaacaaatt gtgtaacaat ttctacacca
    10261 gaatccaaca attttttata cgtgctgata ttttcatctg atgcataggc tctagaagat
    10321 agctgtaatg cattatcttt aacaacagta ccgccaacaa ttagtcggtc aattctaata
    10381 ccgttatcta ctaaatgaag gattgtttcc ggctctttca cgatgacaaa tactttttcc
    10441 tgatcgtatt tccccttttt aaaattcgca atcgctgttt cttcagtgat aattgataca
    10501 gccattccag caggtctagc taaacgcata cttgattttt ggatgtcgtt atttgcgaca
    10561 gaatcatcga tcaccatcac tctgtttgct ttagacattg gtgcccattg cgtgacaata
    10621 atcccatgca ataaacggtt atcaactctc gacataacaa tactcataaa tacttcctcc
    10681 tttatctttc attttcttac aagcaattac catgccaaaa caaaaagaac ttgataaatc
    10741 aagctctttt tacataatca taaacgtatt taacctcatg aatcgggatg tcgacactat
    10801 aataatgttc aacgacactg aaagattgct taaaatgtgt ctgaaatttt tgaaattcct
    10861 cactcgtttc attgaaataa atttcatctt ccaaagtatt ttgttttact aatcgttcaa
    10921 ttaagcaact aatgtgaaca tataatccga aacacgtatt attatcaaat gaaattccta
    10981 gatcattttg taagatgtat atagcatctg aaacccgttc taataattta gtcggattta
    11041 atatggtcaa ttgctccatt aaattcgtta gactaaaatt cttcagaata ttattgttca
    11101 aaactttcag ttcattatca ttaaagtgtc tattcaattg attggagaat ttacttatat
    11161 tcattcctaa aatcatatct tcaattgaaa agaattctac atcttctaga tgtggattta
    11221 gtgttccaac aataaaaaga atattgttct ctttacgaag ctccgataaa tagttttcat
    11281 tcactaaaga gtaatagtca cacgtttcta tgacaagatc agtatccctt ggaaagctat
    11341 cttcaagtat cttttttaat ttaaacgaag ttcctaatcc gcttgcacaa gaacagacaa
    11401 tcatatcctt cttaatattt gatgattcat aaatataatc cacattttga ttttctttta
    11461 cttgttcaag aatctgttta agcggtaaat ctaataatat ctgatttgca acatcaatag
    11521 ccattcttgt tgaaacattg tttagtagtc cgaatttttt ttgagcgtct agttccaaat
    11581 ttagataaat tttctccagt gaacccatat caacaagtaa aataatttct tcatatccgg
    11641 tacactcttg taagtatcta ttaattttgt ctgcaatctc actacttgaa gtcattagtg
    11701 gcatatcaat ggcgtcgtat atatatctct caacgatcct attagcagta cttgctatac
    11761 aggttgccgt tagatctcca tgagccaaaa tcagtgctaa acgtttttgc tgaaattctt
    11821 tactacacat taacttaaat aaaataacca aaacattttt ttgaattttt gttaaataaa
    11881 tatcccctat ttgaataaaa cgattgacta attgaacttc tcg
//
LOCUS       DAYYGO010000088        11914 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000088 DAYYGO010000000
VERSION     DAYYGO010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11914
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.090"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..282)
                     /locus_tag="ACHA5T_002072"
     CDS             complement(<1..282)
                     /locus_tag="ACHA5T_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFM8887631.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQT"
     gene            complement(288..1151)
                     /locus_tag="ACHA5T_002073"
     CDS             complement(288..1151)
                     /locus_tag="ACHA5T_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8887632.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(1165..2061)
                     /locus_tag="ACHA5T_002074"
     CDS             complement(1165..2061)
                     /locus_tag="ACHA5T_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8887633.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(2236..3525)
                     /locus_tag="ACHA5T_002075"
     CDS             complement(2236..3525)
                     /locus_tag="ACHA5T_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8887634.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(3497..5014)
                     /locus_tag="ACHA5T_002076"
     CDS             complement(3497..5014)
                     /locus_tag="ACHA5T_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFM8887635.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            5212..6102
                     /locus_tag="ACHA5T_002077"
     CDS             5212..6102
                     /locus_tag="ACHA5T_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFM8887636.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(6175..6999)
                     /gene="nadE"
                     /locus_tag="ACHA5T_002078"
     CDS             complement(6175..6999)
                     /gene="nadE"
                     /locus_tag="ACHA5T_002078"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFM8887637.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(7011..8483)
                     /locus_tag="ACHA5T_002079"
     CDS             complement(7011..8483)
                     /locus_tag="ACHA5T_002079"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFM8887638.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(9120..9482)
                     /locus_tag="ACHA5T_002080"
     CDS             complement(9120..9482)
                     /locus_tag="ACHA5T_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFM8887639.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            9705..10457
                     /locus_tag="ACHA5T_002081"
     CDS             9705..10457
                     /locus_tag="ACHA5T_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8887640.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(10597..11520)
                     /locus_tag="ACHA5T_002082"
     CDS             complement(10597..11520)
                     /locus_tag="ACHA5T_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8887641.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(11535..11807)
                     /locus_tag="ACHA5T_002083"
     CDS             complement(11535..11807)
                     /locus_tag="ACHA5T_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887642.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         3733 a         2415 c         1953 g         3813 t
ORIGIN      
        1 tgtctgttct tctaataatt gattaacaag aaaataaata atgatcgtta acagactaag
       61 gaacaagttc ttaccaaaat gttgctttat tccttgaaag aaaaaataaa tgatatttcc
      121 tgatccctca cctttccatt gcgccattac ataaaaaaca gcacatgtag caggcacgat
      181 agtaataaca ggcaaacaca cgattaccca gagaatacct aataagatgt aatcagaaac
      241 cacaatcaac tgtccataaa atttagtctc aactattttc atcttcctca ctctttcatt
      301 ccagacatag ctgttccttg aacaaaatat ttctgtgcaa aaatataaac cactagtaat
      361 ggcagcattg aaataaccgt tccagccatt acaggtccat aaaaagaaat gttctgccct
      421 gtaaataaag ctaaccctgc tggtaaagtt ctcatttttg aactacttgt caatatcaat
      481 ggataaagca agtcattcca actattcatc agcgtgaaaa tccctaatga taaaagagta
      541 ggtttgctca atggcaacat gattttcaaa aaaatattaa actcacttaa tccatcaatg
      601 cgtgctgcct cttccaaaga atctggtaaa gtaataaaaa aggaacgcat catgaagata
      661 ccaaatgcag ttgtcatacg aggaatgatc aaacctgcat aagtatcaag catgccaagc
      721 aaattgactt caataaatag aggaatcatg aagacttgga atgggatcat cattgtcagc
      781 aaaacgaaat agaacaaaac tgattttcct ttgaaacgca tacgagcaaa tgcatacccc
      841 gcaagagaat caaagaatac agaagtgatc actgtccctc cagcaaaaat cactgtattt
      901 ttgatgtaat ctaataaagg gatcgttgtc caaaccttaa tatagttatc tagggtatag
      961 ctatctgcaa atattctagg cggaaatgag ataatgtctt tttcgtgttt aaaagaagaa
     1021 ataatcagcc acaaaaaagg aaacgcaata accaacaata taactagcat actaatcgtt
     1081 cgaatgattt ttttaccact aactgctttt cgactattca cagtaactgt aatcgagctt
     1141 tctggtgcca tacaggtttc cctcctattt catttgtttt tctttccgtg acatgtacac
     1201 attagtggag atagctatag ttgcaacaat gataaacaaa accgaagaca gtgctgatgc
     1261 atagcccaat tcataaggtg ctgtaaatcc tcgactgtaa atatattgaa cagctgtttc
     1321 tgtcttaaat tgtgggccac cagcggttgc aacatatact tggtcaaaca cttgcatcga
     1381 accaatcaga ttcgttaaaa tacaaaaacc taaggaggga acgatctgag gtagcgtaat
     1441 gttaaaaaac tgctgtcgat ttgttgcacc atccatttga gcagattcat acaaagaggg
     1501 agagattccc tgaatagcag tcaagagaat cgtcatagta acaccaaaat tcttccaaac
     1561 agtcatgatg gctaccgtcg gcatagcaag ggttggatcg cgaaaaaatt gagggatcgg
     1621 caagccgact tgtgaaaaca agtaaggaat cgcccctaca ttcggatcaa gcaacataga
     1681 aaaaataatc gcaattgacg tcaatgaaca aataactgga atgtaaaatg tcgaacgaca
     1741 aaaacgattg aatctggtat ttttagataa tgctactgct gctaataaac caacgataat
     1801 ttgtgttggc gtttcgagta cggtgaaata taaagaatga atcaacgaat taactgctcg
     1861 ttcatcacta aatatacgtg tgaaattttc aaaacttaca aaatttcttg atgtaaagaa
     1921 aatattcaaa tctgtaaaac taatagcaaa tgttcctaaa agcggtatga cagtaaataa
     1981 taataaaact aaaaaagatg gagttaaaaa caaatacgca gcacgatcag gacgattcca
     2041 atatttggtg aatccgttca tatagtccac ctcatctttc attttgataa aatatacaat
     2101 cttggtttaa acgaatctga ataagatatc cgaacaatcg ctgtgatttt tgcaataaca
     2161 cctatctgca aatactaggt cgatcattca gatatctgta tcttgccaac agtctatcct
     2221 gtataaagtt ctgtactatt tttgcagtaa ttgattgatg gcatcatctg catcgttcat
     2281 gagtgtctca ggatcttctc ctgctaataa tttttcaatc agtggattga tgatatcgtt
     2341 attgatcgca gagatttggg taaaacctgg taaatatggc tctgcatagt ccatttgttt
     2401 cgataattca gatacaatag gatcattttt tacttcttca tcttttgcta ctgaatccaa
     2461 gtaggcagga aaaccatttt cgatactcca ttttttacca atttcagttg tattccaata
     2521 tttcaaaaac tcatagacag cttctttttt attattatct gtacttgccg gaatggcata
     2581 tcctacgcca tctaaaatag ctgttttttt gtctgaatca ctcccttgtg gtaaggtagt
     2641 cacattataa tcaatctcgt ttgctttcaa tccgttattt aaccatggcc cattgatttc
     2701 aatggctagt tgaccagcat tcattaaatt gtccatttcg gcgccagaaa tcgattctgg
     2761 accagtcttg tctacatgga tcattttttg gatcgctttt aacgttttaa ggttctcagg
     2821 agacgcaaat tcagacttag tataatcatc gttcactaat ttaccgccat ttgctaacat
     2881 ccagttatat aacggtgcag ttccatcctt attgaagaca aaacctgata catttttact
     2941 actatcagtt aattttggtg ccatttctgt caattcttcc caagtagtag gtggttgttc
     3001 agtatctaat ccagcttgtt taaacaaatc attattccaa tacatataca tgccttgtac
     3061 ctgcatgggg acaaaatact gtttaccttc tacttcacct aattcaatag ccgtttcttt
     3121 aaaatcagat ttatctactc catcatattc ccagaaatcg tctaatggtt gtaccgctcc
     3181 gttttctaca tactgtgcaa aatctggata atttaatgca ataaagtcag gtgctttttt
     3241 agaagaaatc gctgtaggaa gtttttcatt caaattggcc caagtcatga tatccatttc
     3301 aattttgatg tttttatcat ttgttttatt aaattcatta acgatttctt gcaatatttc
     3361 accatcagat gcagtaaacc cattccaaaa agtcaaagtc acttctttat cagaatcaga
     3421 ttctgctgaa cttccgccac cgcaagctcc taaaatacct gttgcagcta ccaacaaaga
     3481 gccaattaac cattttttac ctttcatttt ttgttcctcc ttcatctgat aaacgacatc
     3541 tgaccacatt ccaagatagc ggtttcaata aacaattgat tttcccatct tcagctttta
     3601 catttgttga atgacttggt tttacttgtt cacttttagg aagattagtt gcttttgtat
     3661 caaatccact catttcagaa aaatcaatca ctccctctaa tgataattca gacaagtgaa
     3721 cagttagatt catttcttct tctccacggt taactgcaaa tatgacaagt tctttgtcat
     3781 ttattacggc tatactttct aaatacggca cttcttcaaa atcttccgtc gcatagtttg
     3841 ctgaacgaat ttgtgggctt aacacctctc cttgtccata cacagcagct tgcataaatg
     3901 gataatagat cgtctgtctc caagcttctc cgtttgtttc tgtcctgatt ggtgcaataa
     3961 cgtttaccaa ttgagcaaga caagcaatct ttactctatc tgcatgtttc aataaagtga
     4021 ttaatagaca gcctaatagt aatgcatctt caaaattata gttatcttct agtaaaggag
     4081 gagcaatctg ccaaggcgct gcttgctcat cttgttccgc tgaatgatac caaatattcc
     4141 attcatcaaa ggacaggttg atctgcttct tactatgttt cttcgccttg actgtatcgc
     4201 acatcgctgt cacttcagaa atgaaccgat ccatgtctaa ggaacgtgca agataatgag
     4261 aaagatcatt tttttggttg ccataatatt gatgcagaga caaataatct atttgatcgt
     4321 acgcttgatc aagaactgtt aactcccaat caccaaatgt aggcatttgc cgattcgaac
     4381 ttccgcataa aaccacctca attgaatcat ctacgagttt catcgcctta gccgtttcct
     4441 ctgctaaccg tccatattct tccgctgttt tatgtccaat ctgccaagga ccgtccattt
     4501 cattccctaa acaccatgtc ttaattccaa aagggctctc ttgaccattc gaacgcctta
     4561 aatcactcca ataagttcct ttaggaaaat tacaatattc gactaaatta cgagctgcat
     4621 ctactccacg agtacccaga ttgacagcca tattgatttc ggcccccaca tgctgacacc
     4681 agttataaaa ttcatgaatc ccaacttcat tcgtttcaat tgtccgccaa gctaaatcca
     4741 accttctagg tctatttgct atgggcccaa tactgtcttc ccaacgaaat cctgacacaa
     4801 aatttccacc aggatatcta atcatgggaa tgttcaattc ttttaccaag tgaatgacgt
     4861 cttggcgaaa tccactttta ttagctgatg gatgatctgg ctgataaatc ccttcatata
     4921 ctgctcgtcc catatgttca ataaatgaac tgtacaaccg cggatcaact ttgcctacac
     4981 gcttgacact actaatcaac attgtggctt ccataactga cctcctttaa tttatacatg
     5041 aataatacac ctcgttgaaa gcgttgtcaa taatataaat gttaaactta cttatttttt
     5101 atcgatttta attaaccaaa atattaataa tgattaaaac aaataattta atataacttt
     5161 tattaacaaa tatgttaata tataagaaaa accttttaag aggtgatata tatgcaacta
     5221 caaataaacg aagaatcctt accggtatat gaagcattag ctagtaaaac aaggatcaag
     5281 atcatccagc tactttccaa aaaaaagatg aatgtcaaag atttggcaaa agaacttgga
     5341 gtgagcagtg ctatcacgac gatgcacgtg aaaaagctag aggaagcaaa catcatcaaa
     5401 acagaaaaag taggacaaca aaaaatttcg agccttcgtg tcgataaaat cgatattagt
     5461 tttccagaaa aaatattcaa tgcttttgat acgaaagaaa catcgatacc tattggtcac
     5521 tatacaaatt atgcaattga accaacatgc ggtttagcta cgatccatga ttttatcggg
     5581 aaagtggatg aaccacgcta ttttatggat cctcgaagga tggatgcccg cattctctgg
     5641 tttaccagcg gatttgtcga gtatcaggca cctaattttt taaatacaga ggatacattg
     5701 gagatgttgg aagtttctgt ggaaatttct tctgaatttc ctttttcaaa tgacaattgg
     5761 ccttcagata tcactttcag cctaaatggt gtggagctag gaacatggac gagccctgga
     5821 gattttgctg atatccgcgg taaatatacg cctgattggt atccagataa tcttaatcaa
     5881 tacggcttgc tgaagacgat ccgaattacc aaacacttga cgaatatgaa tggagaaccc
     5941 ttgtcaaaca tcacgatcaa cgacatccct aaagagcaag acacttggca tttgcgaatc
     6001 gaagtgaaag acgatgccaa acatgtgggg ggctgtactt tatttggtaa aggattcggt
     6061 aattacgatc aggatatcaa attaaaagtt tactacagct gatatttttc ttttctattt
     6121 tataaaacaa aaacagccaa tccctatgaa gggacggctg tttttgcttt attgttattt
     6181 ccagaaatca tccaaaacgg aaattggcaa atgtcgttta tgttgtgtct tgttccacca
     6241 tgcttcgatt ttttcttgtg cttgagcggg aacttctttt ccttctagat aatcatcaat
     6301 ttcattatat gtgacaccta gtgcgacttc atctgcaatc attggtttcc catcttcaag
     6361 atctgcagtc ggaatttttg tgtataattt ttccggtgca ttcaatgctt gaagcaattg
     6421 tttcccttgt cgtttgtcca aacggaataa tggcaaaatg tcggctccac catcaccaaa
     6481 tttcgtgaag aatccagtaa tgttttctgc agcatgatct gtccctaata cggctcctgc
     6541 tctttctcca gctacggcat attgcgtaat catacgttga cgtgctttga tatttccttt
     6601 gttaaaatcg ctgattccca cacctgcttc gcttaatact tgtacttgtg catcaacagc
     6661 tggtttgata tttactcgca gactaacatc gggctggata aagtcaagag cagcttttgc
     6721 atcttcttca tctgcttgtt caccataagg aagacgaaca gcgataaatt gatagttttc
     6781 atcttttgtt tcttcacgca tttcttccat cgttagttgc gccaaacggc ctgctaaagt
     6841 agaatcttgg ccgccgctga tacctagtac aaaagtttta agaaacggat attttttcaa
     6901 atatgctttc ataaagtcaa tacttttacg tatttcttct tttggatcaa ttgttggttt
     6961 gacacctaat tcattgataa tttctgtctg caggctcatt tattctcctc ctatgcttct
     7021 gtatgaagtt ctgctacgct ttttttcatt cgttccatga tagccatttt atggttccag
     7081 cattcagtag acagatcgac tgggtatttt tgcggattca agtctcgttt atactcttcc
     7141 catagtgaat ctagattttc ttttgtatat tctttgattt cgtttagtgc aggtaattcg
     7201 taaactcttt ttccttcgat aaagatatct tgtaaaactg ggcgagctgt aaagtctcga
     7261 acagttttat tgataaacgt atgcactgga tggaacatga agatagcttc ttcttctcga
     7321 gggtcttcat cccataatgt cacgtagtct ccttcagatt tcccatcaaa atttcgagtg
     7381 atgcgccaca cttgtttttt ccctggagta gttacttttt ctgcattgct tgataacttg
     7441 atcgtatcca tcatttttcc ttcatcatct tcgatcgata ctagcttgaa tactgcacca
     7501 agggctggct gatcataggc tgtgatcaat tttgtcccaa caccccatac atcaatttta
     7561 gctttttgca tctttaaatt caaaatcgtt gcttcatcca agtcattcga agcatagatt
     7621 tttgcttccg taaatccggc tgcatctaat tgttcccgaa cacgcttaga tatataggcc
     7681 atatctccac tgtcgatgcg tacgccttgg aagttgattt tgtcacctaa ttctttcgcc
     7741 acgcgaatcg cacttggcac acctgatttc aacgtatcat aagtgtcgac taagaacacg
     7801 cagtctttat gggttttagc gtaggccata aatgcttcat aatcatttcc ataagattgg
     7861 actaacgaat gagcatgggt cccgctagct ggtatcccaa atatcttccc agcacgtaca
     7921 ttgcttgttg catctgctcc gccgatatat gccgctctag ttccccagac tgctgcatcc
     7981 agttcttgtg cccgtctagt tccaaattcc aatagtggat cttctccgat gacagatttg
     8041 atacgagctg cttttgtcgc gatcaacgtt tggaaattga ccatattcaa cagtgctgtt
     8101 tctactaatt ggcagtgcgc taaaggccct tccacttgga tcaatggttc atttgcaaaa
     8161 actagttctc cttcacgagc agaacgaact gttgctttaa attcaaaatt ttgtaaatac
     8221 tccaaaaaac cttcaggata gacttctaat ccacgcaagt aagcaatatc ggaatcattg
     8281 aacgtcagat tctctaaata attgactaac cgttccaatc ctgcaaaaac cgcgtatccg
     8341 tggttaaaag gcatttctcg aaagtaacat tcaaaaacag catgtaaatc agctcgacct
     8401 aattcccagt atgtctgcat catattgatt tgatatagat ccgtatgcag cgtcaaactg
     8461 tcgtctgggt aagtattatt catcttgtat tactcctctt ggcaagtttt catttcctac
     8521 ccattataac aaaaaaaatt aataggaaaa gaaatcttcc attttttctg aaaaagcaca
     8581 tgttttacgg atatttcttg ctaaaaatga actccatctg ctaaaactat cctttcttat
     8641 tggtatttaa cctaaacgag aaagaatact tattttattc aatgtatccg agacacgaag
     8701 aaattagcgt tttctatttt tcttacttga taaaataagt agattttcct actacaatcg
     8761 tagccgttaa gataccgatg attgaacaaa tataaccaaa tgtctctaca acataatcaa
     8821 tggcaaaagt ttttgaagca ataatacgtc tttccttttt tgcttcttat ccccaaagaa
     8881 acggatataa cttattatgt attgaatttt tttggaaagg cgaattatga caagatatac
     8941 cttgcgttga aaaaactcgc ggaaaaaata tcacacgttt ttaaaaagat acaaagtaag
     9001 aaagcagctg tcaatagaat ggaaacaaaa atggcgagac atcaatcagt ctcgccttgt
     9061 tttttattgt atacattaga tatggagcag gttaccataa agatgattat tagtcgttat
     9121 taaatagaat ccttcgtatt tttttgttct atcactacaa tatctttatt tggacgtttg
     9181 aattgtattc cggcacgttc tagctcctgt ttcactccta catggacttt gacatcttct
     9241 cgtgtcattt ttaaaacgtg atgaatcgca tagtcgattt cttgccttgt gatttttttc
     9301 ttcgtattga tcatcacgac ttgcttttta tcatatgtgt ctaccaatag cagttgtgtt
     9361 cgatctaacg aaaataattt gtaataatta atagccgtat tcccaaaaaa gaattccact
     9421 actttaggat aaaatgtctt aatatttaaa ttactattca caggtgtttc gattagcttc
     9481 atctcattcc tcttttctat atagtcgatt tagatccaat cttaagttat gttaccatct
     9541 ttcctagcat taaggaatcg gttacaattg aacctttaaa gacaaaagga tttacctttt
     9601 atcacttaat agtataacat aatttcatga gaaaaaaata caattttttc ttgaccatct
     9661 cgttacgtaa aggattatgc ttacatttgt caggaggaaa aataatggag tacacaataa
     9721 agaaaatgtc agagatatca ggagtcagtc cgagaacctt gcgcttttat gatgagattg
     9781 gactgctaaa accagctagg atcaattctt caggttatcg gatttatggc aaaaaagaag
     9841 tcgatcgtct gcaacacatc ttattttatc gaacaatggc attcaagttg gatgatatcc
     9901 aagaggtctt agacaatcca tcttttgacc accagaaagc tttaatcaag catcagcaaa
     9961 tgttgctgga aaaacgggca caaatcgata ccctcttgac aactgtccaa cagacacttg
    10021 atatgtacga aggaggaaga aaaatgagtg acaaagaaaa atttgaaggg tttaaacaac
    10081 aaaagttgaa agaaaatgaa gaatcctatg gacaagagat tcgacaaaaa tacggaaaaa
    10141 aaaaggttac agcatccaat gaaaaatgga tgaatatgga taaagaaacg tatgaagaaa
    10201 tgcaaactgt tgaaaagcag atgttatttg atctcactct ttacttaaaa gaaccagaag
    10261 atgaaagctt agcagaccgt atcttccaag ctcataagaa gtggttatct tacagctggc
    10321 ctgaatatca gccaaaagcg cataaaggac taggaatgat gtacgaagcc gatgaacggt
    10381 tcactgcata ttatgacgag cgaagtggtc atggtgcagc aaaagcttta aatgaaatta
    10441 tccaaaaaaa ggcgtaatct tttacatgat ccaacaaaaa agtacacgta aaatgaccta
    10501 ataacaaaac agcagacacc caagttagat ttttatgcta acttttggtg tctgctccat
    10561 tctttcacaa gttttttcat cctactttaa atatattcat cgttgaataa acgtataagg
    10621 tattttcact ttatcctttt tttctgtaag aaacaattga aatgccattt ctccgcaagc
    10681 ttccagatgg tggtcgatgg ttgggaaacc aagaagctcg ctgatcagca aattatcgcg
    10741 tccaatcacc actggtgtct tatcttgcag atagctttgc aagatgcctg ctgcaaccat
    10801 atccgaattt gtaaagacac catcaactcc aagctcttta aaaaagaagc ctgcttgttt
    10861 tcctttctct gcatctatac aatcccaaaa aacaagtgat tcatcaaaag atggaaaaag
    10921 ttgatgacat agctgcaaag tgatttttga gttataactt agcctgccac ttcgccccaa
    10981 cgtgatgccc aattgcttta taccttgctg cttcaaataa gtaagacctt ctgtaattga
    11041 ttgttctcga tcaataaaga cacagcttgc ctgttttccc ttgatttctt cacaaaaaac
    11101 tagaggtcca tatttttgat atgccaataa atcatctagt gtattggcac gagacgtaat
    11161 gatcaatcca tcgaaagctt tagctgcaaa ttcttcaaga taccttcgct ctttttcttt
    11221 ctgatagccc gtcggtaaaa gtgtcacttt gtaatcataa tcaaacgctg cttcaatgat
    11281 cccactgatc aactgatcaa aatacggatg attggtataa ggcagaatca ctccaatgct
    11341 tttcgttttt ccataactta actgtctggc atttgcatta gggatataat cgacttcttt
    11401 catgaccgca agtatttctt tccttttttc ctcatctaca tatggatgtc cattgattac
    11461 acgtgaaata gtagaaactg aataacctgt gagctttgcg atatctcgaa tgtttgccac
    11521 tgctgcttcg ccacctatac aaaaagattt tcttgtaaat agtctaccac agctggaccg
    11581 cattcaaaaa actgatacat ttttccttca tgcgaaaaac tgatccgctg cccgccccaa
    11641 tatatatctt ttgtcaaatt ttcgatttca ttcattggta tttcttttac tatattccta
    11701 ttcaaccctt gaaaactgta caacgttaat acggatccct tgaactcgac aaacgcataa
    11761 aaataattgt tcgttgtgcc atctgttatc tctacataag tattcattgc aagccctcct
    11821 tttgatactt tgagtatagt gtatgaaacg cgtctcatag caaggagttt gttttgccaa
    11881 agaaaagttt tattcctctt tcattattac caaa
//
LOCUS       DAYYGO010000089        11765 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000089 DAYYGO010000000
VERSION     DAYYGO010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.091"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..892)
                     /locus_tag="ACHA5T_002084"
     CDS             complement(<1..892)
                     /locus_tag="ACHA5T_002084"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8887643.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDS"
     gene            complement(1269..1907)
                     /locus_tag="ACHA5T_002085"
     CDS             complement(1269..1907)
                     /locus_tag="ACHA5T_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8887644.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDFVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFNQETKHLTGREKRLIKRNALSFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKEQVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(1911..3893)
                     /locus_tag="ACHA5T_002086"
                     /pseudo
     CDS             complement(1911..3893)
                     /locus_tag="ACHA5T_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
     gene            4073..4738
                     /locus_tag="ACHA5T_002087"
     CDS             4073..4738
                     /locus_tag="ACHA5T_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8887645.1"
                     /translation="MNIGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHQTLGLPIDFPSIESATESIEPTLPDEPLVIKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETVDRFNPFLKPQIGYTTLPVGYVENSIEHWVCNDIFVHGTW
                     LSFHAGEVEKGKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILIQDYDDALKRSPQEP
                     DRH"
     gene            complement(5135..7168)
                     /gene="brxL"
                     /locus_tag="ACHA5T_002088"
     CDS             complement(5135..7168)
                     /gene="brxL"
                     /locus_tag="ACHA5T_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HFM8887646.1"
                     /translation="MEQQEILKLSEFFPGKIVRKDLTKKIKEGANVPVYVLEYLLGMY
                     ASSLDETLVEKGVENVKNILARNYVRPDEAEKVKAKIRELGGYTVIDKVTVHLNEKKD
                     CYEAEFSNLGLKNVQISEEYPKKFDRLLGGGIWCIVSMEYWYDENDRWNSPFIAKKVD
                     PIQMPGLDIEAYKMARKEFTTNEWIDLLLRSIGMEPTQFNKRVKWLHLARLIPLIENN
                     FNLCELGPRGTGKSHVYKEISPNSILVSGGQTTVANLFYNMASRTMGLVGMWDCVAFD
                     EVAGIKFKDNDGVQIMKDYMASGSFARGKEEKNASASMAFVGNINQSVDVILKTSNLF
                     EPFPEVMGTDSAFLDRMHCYLPGWEIPKYRPDFFTNDYGFITDYFSEVMRELRKVSFG
                     DSFDKYFKLGSQLNQRDTIAVRRIVSGMMKLLYPDGRFEKSDVEEILRFALEMRRRVK
                     EQLKKIGGMEFYDVNFSYIDLEEEREEYVSVPEQGGGTLIPEGLGKPGHLYTVSYGKT
                     GQLGTYKLETQMTSGNGKFERTGINSDAGAREAMDSAYRYLRANYGSVSQSISLTTKD
                     FVVHVQDMNGIGMTKTLSLSTIIALCSVSLNKPVLPSLVILGDITIGGSMIKVDNLAN
                     CLQVAKDAGAKKVLVPMISAADIGTVPPELMGTFNLIFYQTPEDAVFKALGVE"
     gene            complement(7286..9808)
                     /gene="pglZ"
                     /locus_tag="ACHA5T_002089"
     CDS             complement(7286..9808)
                     /gene="pglZ"
                     /locus_tag="ACHA5T_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HFM8887647.1"
                     /translation="MTDINTQQIEEKLNQRFPNYGCRKIIFWFDSNQDFLEEIDSLVI
                     QNAKIYRLEKDAQFKTKRLLEYEDPETNYLIYAPFPRWDDKDEDNHLLSVLNYSEEFS
                     ADRLAIIMNELAIPLRFHQTVKQYRNFFNAKDRMRSFGEFSTHMTINKSETIELMIMA
                     VLLKSKSIRLNDLLRVVLKSFTESRENPLRELDKYQLTAKFWEFIQMKFGFTDSQPTL
                     EKLTMCLYLNYFYYQIDQEMPKKFKGFEILHLKANIITFMDQFMNDTRYMESFEQLSK
                     KVYQAIDGDGLLDSIEIDDLIEADVFSEIHAKILMFYAERLKASDTTTKIAGQTIFDS
                     LKQREKMHFASDYIYEYQLLKHAYYLVDKNRLCLPMNMDNLLTQYEKEMYWVDTHYRK
                     FIWNSDHIEEKQKYGQLYRLVEANYSKFLDQSGQIWNQTFEFKNRPSIRKFYNQAVMK
                     RSVKTVVIISDAFRYELGMNLERKLVREKKYSTNMASFLSVLPSVTEFGKAALLPNNQ
                     LTYNEGTEVLIDGKKTNGLSNRNAILQQRDANAVALNYDDVVKMNKAEVREVFNGKSV
                     IYIYHDQVDRTGDHSNENRVFEACDLAISEISRLINRLHNDGSIYRFIVTADHGFIYK
                     RSHVEEVDKIENPSRNEKDRVERRFILSEEEYEIIGTKNYSLGDSLGNDDTRNICVPI
                     TSSIFKKAGGGQSYVHGGSSIQEMLVPVLEITVAKGSNAKEPVAIELMTSNRRITGLS
                     TTLEFYQKNAISDVYDPASFWLYFEDEYGERISNEEIYVADSKTEDANQRFTKFVFEF
                     VNRRYTVEDTYYLVIKNQKNDLTERIKFIIDNPFAESFDFDI"
     gene            complement(9924..>11765)
                     /gene="pglX"
                     /locus_tag="ACHA5T_002090"
     CDS             complement(9924..>11765)
                     /gene="pglX"
                     /locus_tag="ACHA5T_002090"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HFM8887648.1"
                     /translation="ATVPICTFTIKNSNEQDGSYIKLSDFKGGMEVQKIKTLEAIHQH
                     GECDYFYSTNQDNFKKIPGMPIAYWASDNVITDFAQGKLTNDVADAKQGLATGNNNKF
                     LRYWFEVEFNKIKFDAKTLEDAKKSRKKWFPTTKGGRFRKWYGNNEYVVNWENDGDEI
                     KNYVNENGKLKSRPQNLNYYFKKGITWSTLSSGQVSFRLQNQAIFETKGSVLFSKDIT
                     DFEEIFGYVNSKLVNHFLTFISPTLDYHEGPMGKLPFLKVTSERKLKKIREKVDENID
                     IARKDYNALETSYEFRRNILLSYMFKKDLSEAFNCLSRQLVNQFNTLKSNEEELNDIF
                     INHYSLEKELTKEVQDEYISARLPDKAREIKDFISYSIGCIFGRYSLDEDGLIFAGGE
                     WDPSRYQKFKPDTDNVLLITDDEYFKDSQIDIVNRFIDFVEITFGKETLEENLQFIAD
                     TLGGKGTSREIIRRYFVQDFYKDHLKTYSNRPIYWQLDSGKQNGFKALMYLHRYTPDQ
                     LGKVRTEYLHELQKAYDNRIELRQQQISTSDNPKERNAMTKEIVKIQKQLKEVREYDE
                     KLGHLALKRIELDLDDGVIVNYDKLQRDPKTGEKFQIFAKGVKEPKK"
BASE COUNT         3704 a         2266 c         1701 g         4094 t
ORIGIN      
        1 tagaatctga agtgaatgta ctcaaaacat tttcaaagtc tttgtctttg atttttacat
       61 tagcgtcttt caatacttcg ccaattactt tattctggaa ggtactgtca cttaattttg
      121 tgtctgttgc aatttctttc aattcttttt tgtatttatc catgtcgttg cctttgtttt
      181 ggtttttcac cattttaacc acataatatt ccgtagtata agttgaagca ttggtagaag
      241 tgatcacatc agaaacttga ccatctttca acttaaaggc tgcttctttg acttctgccg
      301 gaaccgttgt agtagtggaa tcaaatttca cttttccgcc atcttctttt gtcgtagtat
      361 cagttgactt gtctttggct aatttagaga aatcgtctcc cttatcagca gctttcttca
      421 catcttttgc atcatcttca cttgctactt ggataatttg agcttccact tctggatgga
      481 aagattccca agccgttttt aaatcttcat cggtcaattt gatatgtttc tttagacctt
      541 cttgaaaggc tagactttgt ttgatactgt ctttgaatgt ttgttctgtg taacctgcag
      601 atttcaattg tgaatcaaat gaatcaccca attgtttctt tgtttgatca tattgggaat
      661 cgatttcttt ggttgatacg tcatctccat atttatcttc aaatacttga taaacgatca
      721 actgttgcac agcggaatct gctccttgca tttttttcaa gctatccatt acttcttgtt
      781 cgctgattgt accgccttta taactaacgg ctgttttttc accggcattc gtacagcctg
      841 acaataagcc agtgcctagt acgatggcaa gtgctaaaaa tctttttttc atttatcaat
      901 tctcctactt ttcgtttttt gcgaattgct aaaaacaaag gtgttcaaga tgaaaatcat
      961 caagaacacc tttactaatc tgctcgatac gtcctgaata gatataaggc tacacaatcc
     1021 agaaagttcg ctcagcagaa taaatgtcaa gtagcacatc acaccttaat atactacttc
     1081 ttttgttacc gtttttcaag tctatcttgc gttccgtatt taacgtaact aattcttaga
     1141 acaaatgtta tcgattttgt ttttcttctt ccgctgttgg gatattcaaa tcagacactt
     1201 ttatgttcga ttttccagac atttttttgt tgtttttatt agaaaaagtt tgttttttca
     1261 tttgttttct aaagcatgat cttcttgttt cctattttgg tcagatatgg atcatgcgtg
     1321 accacgataa ctaacgtacc ttgggaagcc tgttgcgata agatttcaac gacttgctcc
     1381 ttatttcgtt tatctaatga tccagttggt tcatcggcta aaatgatttt gctattcttc
     1441 aataagactc tcgcacaggc gattctttgc ttctcaccac cagataatgt gtagataggc
     1501 gtacttagct tcttgtttat atttactttt ttcaatgcct catactttgc tgttcgctta
     1561 tctgaaacat ttaaattcga aaacttcaat ccaatatcta aattaaaatc gattgtttca
     1621 tattcaatca acccaaaatc ttgaaaaaga aaagagaggg catttctttt tatcaagcgc
     1681 ttctctcttc cagttaaatg ttttgtttct tgattaaata aataaaattg ccctgaactg
     1741 aattgatcat tcaatcctaa tatattcaat aaagtacttt tccctgctcc gctctctcca
     1801 actacagtca caaaatcccc agcatccgca gtgaaacgat ccaaatgaag aatcgttttg
     1861 tttcgaatga tttttttcgc accttttatc actatcaacg gctccaattg ttaacctcca
     1921 ttcatcagca ataactgact tttcttttct gtagacaaaa taaaaacaac tgaaagaata
     1981 aaatcaatga agctaaagaa aaaaatatta aaaagatatt taggctctct ttctataaga
     2041 aaaaggaaga ttacacttgt cgttccccat ttgatgaggt tcatcatgaa gaaatcttta
     2101 tacgtttcaa agagcgaata accacttgaa cgaaagatag cgattttccg acaattgtag
     2161 acaaaatata tgcataacat gacatagctc aacaagaaga aagaaaagaa agtgagtaaa
     2221 aatatctgta tcaagtaatg aaatgatctg cttaacactt tgacagctat ctcttcagtc
     2281 tgagtatacg gaaatgataa ctgcaagtta tcacgtaact gactttcttc aatcagctca
     2341 aataaccttt ctttcgacaa agagcctatc ttgatcttta acggtggata gatttctccg
     2401 ctgagaatat ttctttccca atacgtactg tttttcaatg tcagtatttc aacaaccgga
     2461 taatgttcga tccaggggat agaaggaata aatgtataaa tgggttgatt gttttttata
     2521 aatagaaatt tcggttcttt ttcactgatt tcttgataat attctgttat ttgcgccagg
     2581 tcatttttta aatctgtatg tctatatcgt tcagggatca atataacagg gtgcgtttct
     2641 ttttcggtta tttggatcaa ccttccagag acatcaatca aaggaaattt cttaagataa
     2701 ttcagattta tttttatccc tctgccaaac tgttcatttt cttcattcaa gtaatctgat
     2761 acattgacta atatacttcc gtttttattc aaagtttcat ataattcctg atcggctgct
     2821 gatgcaaaac gattggaata aataaaatcc acgtggtttt tcccgatggt tactggataa
     2881 aatacatgat agtcctcacc agcgatttct tttggtgttt catgggaaac agctaactgt
     2941 gaagaagaaa aaacgatagt tgctaattcg acattggcaa cggtcaaaag aagagaaaac
     3001 aacaatccaa atgtaaaaag aatgtattgg atatagttga acgcttctct taacgaagcg
     3061 attttttcaa ctaataaaag agaaatcaaa gagataccct atattccgct gaacaagaaa
     3121 acgtacatct ggaacaaata aagaaattgc tccttataat acataaaaat aaaaccacca
     3181 atgattcctg caattatgaa tgtaataatg attttctttt tccccaataa acaccacaat
     3241 acttgatgta atgaaaagcc attattcaac aataaactga tttctttatt catcaataag
     3301 aaaaaggatg ataacaaaag gaataaaaga atcaatccaa tagttaaata cactcgataa
     3361 ttatatacgt ctgtacttga aataaccgaa tacgaatagc tattttcaaa gtcttcaaaa
     3421 atattagtgg cataatcagt gtgaaatgct tgattgaatc gttccacaaa tagtaaaatg
     3481 aaccgttcat aggcttcttt ttctctcgtc atcaagaaaa attcaccttc aaaatctttt
     3541 attttgatcg attcttcaat ggacgttacg cgaaaggagg tattagaata gggtaccttc
     3601 tcgtgagaag atttgacatc cccttgataa tacaatgtca tttcaagctt tggctgtatc
     3661 ttgaagttcc aagatgtatc tacttcattg gattcattta ctgttttttt catataagaa
     3721 agctcagttt ctgatgagac attttcaaat accatgatta gctgctcata ttcttcttgc
     3781 ccaataaaat gttgtggcaa tcgaatcgga gtataagtat ctgtttgatg gaaaaattca
     3841 tttgtccgat agagtacctg tttatttata ataaccaagg caaaaccact catgataccg
     3901 gtcactatga ttgtgagtaa aactgtgaac ttttttttga ttatcaccac tctcccttac
     3961 ttaaatgatt ctttgataaa atcataccgt ttttcatcaa aaaaacgtat ctatcaaatg
     4021 tataggtcat gtagaataaa tctatatact gtagaatcga aaggagaaga agatgaacat
     4081 cggagaaaaa atacgaactt accgcagaaa ttgtaagctt acgcaaaaag aattaggcga
     4141 aaaaatcggt gtttccgata aaacaatctc tagttgggaa aattctcgta caatgccaga
     4201 ccttgaaatg ctgtcacttt tacatcagac tttaggtctg ccgattgatt ttccttctat
     4261 tgaatcagct accgaatcaa tagaaccaac attgccggat gaacctcttg ttatcaagaa
     4321 atcttattca tcaaaacaac ccttctcact caaaaagaca ctatctttct ttctgctcgt
     4381 cttttgtttg gtctatcttt ttcttatcaa ttatcaatat aaaaatgaaa cggttgatcg
     4441 attcaatcct tttttgaaac cacaaatcgg ctataccaca ctccctgtag gatacgtgga
     4501 aaattccatc gaacattggg tatgtaatga tatctttgtt catggtacat ggctgtcttt
     4561 tcacgctgga gaggtagaaa aaggtaagaa tcatgctgta atcaaacaca aaggaaaaca
     4621 cgtcatgaac gttcgcctgc tcacttggga agccattccc tcagattacc aagaaatcct
     4681 catccaagac tatgacgatg ccttaaaaag aagccctcag gagccagata ggcattaagt
     4741 aggagatgaa cgtagagata gaaaaaatca atgcacatga tcttatcaaa aaaaaacagg
     4801 ggtacaaatt aattgaaaaa gctttgagat gaaagttcct aaataaaaaa gttcagaatg
     4861 ataggtaaaa aaatgactaa ctattctagt attaggctat ttgtattatt cttctgttat
     4921 ttttatatca aaaaatccag aaataactaa atataggtat ttctggctta tagtataata
     4981 tttacgccac agcccccaat gattcttttt ggaatcatga tcaaggacgg acatacacaa
     5041 aaatattttg cccattcgat ccgaatagat taacgactaa tctagtaagt aatatagatt
     5101 ggtacgtagt caaaccagca aatggaaaag ttgtctattc caccccaagg gctttaaaga
     5161 cagcatcttc cggcgtttga taaaaaatca aattaaaggt acccattaat tctgggggta
     5221 cagttccaat atcagcagcg cttatcatag gtacaagtac ttttttcgct ccggcatctt
     5281 ttgctacttg taaacagtta gctaaattat ctaccttaat catagaacca ccaattgtaa
     5341 tatcccctaa aataactaaa cttggtaata caggtttatt taaggaaact gagcataaag
     5401 ctattatagt tgataacgat aatgtttttg tcataccaat gccattcata tcttgaacat
     5461 gaacgacaaa atcttttgtc gtcaaactaa tagactgact aacacttcca taattagctc
     5521 ttaaatatcg gtatgccgaa tccatcgctt ctcttgctcc agcatctgaa ttgatcccag
     5581 tacgttcaaa ctttccattt cccgaagtca tttgcgtttc caatttatag gtacctaatt
     5641 gcccagtttt gccataagat accgtgtaca aatgccctgg cttccctaac ccttctggaa
     5701 tcaaagttcc tccaccttgt tcaggaacag aaacatactc ttctcgctct tcttctaaat
     5761 caatgtatga aaaattgaca tcataaaatt ccattccacc aattttcttt aattgttctt
     5821 ttacacgacg acgcatttca agagcaaatc ttagtatttc ttcgacatca cttttttcaa
     5881 atctaccatc aggatacaac agtttcatca tcccagatac aatccgacga actgcaatcg
     5941 tatcccgctg atttaattga gaaccaagtt tgaaatattt atcaaaacta tcgccaaaac
     6001 taacttttcg aagctcacgc atgacttcag aaaaataatc tgtaatgaag ccatagtcat
     6061 ttgtaaaaaa atctggtcga tatttaggaa tttcccaacc tggtaaataa caatgcatcc
     6121 gatccaaaaa ggctgaatcc gtccccataa cctccggaaa tggctcaaat aaattcgaag
     6181 ttttcaatat tacatcaacg ctttgattaa tatttcctac aaaagccatt gatgctgaag
     6241 cattcttttc ttctttgcct ctagcaaaag atcctgaagc catgtaatct ttcatgattt
     6301 gaacgccatc attatcctta aatttaatcc cagcaacttc gtcaaaagca acacagtccc
     6361 acatacctac aagtcccatt gtacgactag ccatattata gaaaaggttt gctactgttg
     6421 tttgtccacc tgaaacaagg attgagtttg gtgaaatttc tttatataca tgcgatttac
     6481 ctgtaccacg tggtcctagt tcacagagat taaaattatt ttcaattaat ggaataagtc
     6541 ttgctaaatg aagccatttt acccttttat taaattgtgt aggttccata ccaatggacc
     6601 gtagaagtaa gtcaatccat tcatttgtag taaactcctt gcgagccatc ttgtaagcct
     6661 caatgtctaa accaggcatt tggattggat caaccttttt tgcaataaaa gggctattcc
     6721 aacgatcatt ctcatcatac caatattcca tcgaaacaat acaccagatc cccccaccta
     6781 ataatcgatc aaattttttc ggatactctt cactaatttg aacatttttt aatcccaggt
     6841 tagagaattc tgcttcataa caatctttct tttcatttaa atgtacagtt actttatcaa
     6901 taactgtata accacccaat tcacgaattt ttgcttttac tttttctgct tcatcaggac
     6961 gcacatagtt tcgagcaaga atatttttaa cattttccac acctttttca accaatgttt
     7021 catccaatga cgaagcatac ataccaagta aatattccaa tacataaaca ggtacattcg
     7081 caccttcttt aattttcttt gttaaatctt ttctgacaat ctttccggga aagaattcag
     7141 ataactttaa aatttcctgt tgttccacta gaattcctcc atgtgttcag tcattataat
     7201 agcaattaaa gtatatcata cttaaccagt aggcttgata atacaaaaga aaaagtatcc
     7261 acaaaaaatt gtggatacct cgtttttata tatcaaaatc aaatgattct gcaaaaggat
     7321 tatctataat gaattttatt cgctcggtca aatcattttt ttgatttttt atcactaaat
     7381 aataagtatc ttcaactgta tatcgacgat ttacaaattc aaaaacaaat ttagtaaacc
     7441 gctgatttgc atcttctgtt tttgaatccg caacatagat ttcttcattt gagatacgtt
     7501 caccatattc atcttcaaaa taaagccaga aagatgcggg gtcataaaca tcactaattg
     7561 catttttttg atagaattct aacgtggttg ataatcctgt aattctgcgg ttgctagtca
     7621 ttaattcaat tgctactggt tcctttgcat tgctaccttt ggctaccgta atttctagaa
     7681 cagggactag catttcttga atagatgacc ctccatgaac gtagctttgt cccccgccag
     7741 ctttcttaaa aatacttgaa gtaatcggga cacaaatgtt tctagtgtca tcatttccta
     7801 aagaatctcc caaagagtaa tttttcgtac caattatttc gtattcttct tcactaagaa
     7861 taaaacgacg ttctacacga tctttttcat ttctagatgg attttcaatc ttatctactt
     7921 cttcaacgtg gcttctttta taaataaatc catgatcagc cgtaactata aaacgataga
     7981 tactgccatc attatgcaag cgattaataa gtcgggaaat ttcgcttatc gctaaatcac
     8041 aggcctcaaa aactcgattt tcattcgagt gatcacctgt tctatccact tgatcgtgat
     8101 aaatataaat gactgatttg ccattaaata cttctcgtac ctcagcctta ttcatcttaa
     8161 ccacatcatc ataatttaaa gctacagcat ttgcatctcg ttgctggaga atcgcatttc
     8221 gattggataa accattcgtt tttttcccat caatcagtac ttctgttcct tcattgtaag
     8281 ttagttgatt gtttggtaat aaggcggctt taccgaattc agtaactgaa ggtaatacag
     8341 aaagaaagct agccatgttt gttgaatact tcttttctct aactaactta cgttctaaat
     8401 tcatccctaa ctcatatcga aaagcatctg aaataataac tacagtttta acagagcgtt
     8461 tcattacagc ttgattataa aattttcgaa tggatggacg attcttaaat tcaaaagttt
     8521 gattccaaat ttgtcctgat tgatcaagaa acttcgaata atttgcttca actaatcgat
     8581 acaattggcc atatttttgc ttttcttcta tatgatcact attccaaata aatttccgat
     8641 aatgcgtatc aacccaatac atttccttct catattgagt tagcaaatta tccatgttca
     8701 tcggcaagca caatctgttt ttatccacaa gataataagc atgtttcaat aattgatact
     8761 cataaatata atccgatgcg aaatgcattt tttcgcgctg ctttaaacta tcaaaaattg
     8821 tttgtcctgc aattttcgtt gtagtgtcag aagcctttaa tcgttctgca taaaacatta
     8881 atatttttgc atgaatctca gaaaatacgt ctgcttcaat tagatcatca atttctatac
     8941 tgtctaataa tccatcacca tcaatagcct gatatacttt tttagatagc tgttcaaagc
     9001 tttccatgta acgtgtatca ttcataaact gatccatgaa agtaataatg tttgctttca
     9061 aatgtagtat ttcaaatcct ttgaacttct tcggcatctc ttgatcaatt tgataataaa
     9121 aatagttcaa ataaagacac atcgtcagct tttccaatgt tggctggcta tccgtaaagc
     9181 caaatttcat ttgaataaat tcccaaaatt tagctgttaa ctgatactta tcaagttccc
     9241 ttaaaggatt ctccctactt tctgtaaagc tttttagaac tacacgcagt aaatcattta
     9301 atcgaatact tttcgatttc aacaaaacag ccattatcat taattcaatc gtttctgatt
     9361 tattaattgt catatgcgta ctaaattcac caaaacttct catgcgatcc tttgcattaa
     9421 agaaattacg atactgttta actgtctgat gaaatctaag tggaatagcc aattcattca
     9481 tgataatcgc taagcggtct gcactaaatt cctctgaata attcaataca ctcaacaaat
     9541 gattatcttc atctttatca tcccaacgag gaaatggcgc atagattaaa tagttagttt
     9601 cgggatcttc atattccaat aaacgcttag ttttaaattg tgcgtccttt tctaatcgat
     9661 agatttttgc attttgaatg accaatgagt caatttcttc aagaaaatcc tgatttgaat
     9721 caaaccaaaa aatgattttc ctacatccat aattaggaaa acgttgattt aatttttctt
     9781 caatttgttg cgtatttata tctgtcatca atctcaccta atcgttctct ttctataccc
     9841 cttatattga cataatttgg ataatttcgc acgcaattta agaataaaaa agaaagttgt
     9901 tagaaaacaa ctttcttaga taactatttt tttggttctt tgaccccttt tgcaaaaatt
     9961 tggaatttct cgccggtttt aggatcacgc tgcaacttat cataattgac aattacacca
    10021 tcatctaagt ctaattcaat tcgttttaat gctaagtgac ccaacttctc gtcatactca
    10081 cgaacttctt ttaattgctt ctgaatcttt acaatttctt ttgtcattgc atttctttct
    10141 tttgggttat cacttgttga aatttgttgc tgacgcaatt cgattcgatt gtcatacgct
    10201 ttttgtagtt cgtgtaggta ttcagtacgt acttttccta attgatctgg tgtatagcga
    10261 tgcaagtaca ttaacgcttt aaagccattt tgttttcctg agtccaattg ccaataaatt
    10321 ggtcgattgg agtatgtttt taaatggtct ttgtaaaaat cttgtacgaa gtaacgacga
    10381 ataatttctc gacttgtccc tttgccacct aaagtgtcag cgataaactg gagattttct
    10441 tcaagggttt ctttaccaaa agtaatctca acgaaatcaa tgaaacgatt gacaatgtca
    10501 atttggctat ccttgaaata ctcatcatcg gtgatgagta aaacattatc agtatcaggt
    10561 ttaaattttt gatagcggga aggatcccat tcgccgccag cgaagatgag accatcttcg
    10621 tctagggagt aacgaccgaa gatacaacct attgaataag aaataaaatc cttaatttcc
    10681 ctcgccttat caggtaacct tgcagagata tattcatctt gaacctcttt tgttaattct
    10741 ttttcaagag aataatgatt aatgaatatg tcgttcagtt cctcttcatt cgattttaga
    10801 gtattaaatt gattaaccaa ctgcctactt agacagttaa atgcctctga gagatctttt
    10861 ttaaacatat aacttaacaa aatgtttctt ctaaactcgt atgacgtttc aagagcatta
    10921 tagtcttttc tagctatatc gatattttca tccacttttt ctcttatttt ttttaatttt
    10981 ctctcagatg taactttaag aaaaggtagt tttcccattg gtccttcatg gtaatccaag
    11041 gtgggagaaa taaaagttaa aaagtgatta actaatttag agtttacata gccaaatatt
    11101 tcttcaaagt cagttatatc ctttgagaac agaacagatc ctttagtttc aaaaattgcc
    11161 tgattctgca atctaaaaga tacttgtcca gatgaaagtg tcgaccaagt aattcccttt
    11221 ttaaaatagt aattaagatt ttgtggtcta gattttaact tcccattttc attaacataa
    11281 ttttttattt catctccatc attctcccag ttaaccacgt attcattgtt tccataccat
    11341 tttctaaaac gaccaccttt agttgtagga aaccattttt ttctagactt tttagcgtcc
    11401 tctaatgttt tagcatcaaa ctttattttg ttaaattcta cttcaaacca ataccgcaaa
    11461 aatttattgt tatttccggt tgccaaccct tgttttgcat ctgcaacatc gttagttaat
    11521 tttccttgag caaaatcagt gattacgtta tcgcttgccc agtaagcaat cggcattcct
    11581 gggatttttt tgaagttgtc ttgattagta ctataaaaat aatcgcattc accatgctga
    11641 tgaattgctt ctaacgtttt aattttttga acttccattc cacctttaaa atctgataat
    11701 tttatatagc ttccatcttg ttcatttgaa ttttttatcg taaacgtaca aattggaacg
    11761 gttgc
//
LOCUS       DAYYGO010000090        11511 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000090 DAYYGO010000000
VERSION     DAYYGO010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.092"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(207..692)
                     /locus_tag="ACHA5T_002091"
     CDS             complement(207..692)
                     /locus_tag="ACHA5T_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFM8887649.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    782..1127
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1172..1813
                     /locus_tag="ACHA5T_002092"
     CDS             1172..1813
                     /locus_tag="ACHA5T_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8887650.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            1832..2467
                     /locus_tag="ACHA5T_002093"
     CDS             1832..2467
                     /locus_tag="ACHA5T_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8887651.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2485..3300
                     /locus_tag="ACHA5T_002094"
     CDS             2485..3300
                     /locus_tag="ACHA5T_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8887652.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3619..5055
                     /locus_tag="ACHA5T_002095"
     CDS             3619..5055
                     /locus_tag="ACHA5T_002095"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8887653.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5244..7691
                     /locus_tag="ACHA5T_002096"
     CDS             5244..7691
                     /locus_tag="ACHA5T_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFM8887654.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7921..8988
                     /gene="rlmN"
                     /locus_tag="ACHA5T_002097"
     CDS             7921..8988
                     /gene="rlmN"
                     /locus_tag="ACHA5T_002097"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFM8887655.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            8978..9184
                     /locus_tag="ACHA5T_002098"
     CDS             8978..9184
                     /locus_tag="ACHA5T_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887656.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(9181..10284)
                     /locus_tag="ACHA5T_002099"
     CDS             complement(9181..10284)
                     /locus_tag="ACHA5T_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8887657.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            10836..11384
                     /gene="aac(6')"
                     /locus_tag="ACHA5T_002100"
     CDS             10836..11384
                     /gene="aac(6')"
                     /locus_tag="ACHA5T_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFM8887658.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         3820 a         1946 c         2485 g         3260 t
ORIGIN      
        1 gaaaaaagaa gcatttttat atatgcttct tttttcgtag aatcaataaa tatggatttg
       61 gctaactatg gaagaatact cgtattagtt ttcagcgttc atactagttg ttacattgtt
      121 tgattggcca aaagaatttc tgatatattt tgtgctaaat aaagaaaaag acgcttgaat
      181 tgggaaataa gaaaactact ctatcattac aaattttcat aaactggcag atgttctaag
      241 taaacaagta tcttttcaaa gtcggaaaaa tccataatca acgtagtggt attgatattt
      301 ggatgaaagc ctacggtatc ttgacgatca atagcagcat cgatcacgac ttggattttt
      361 ttctctttgt cgtgcatcaa tgctaatgga gtcactgtac ctgctggaac gcctaggagg
      421 tcaggaagct gttcttcaga aacaaaagat aagcgctttt cttttaattg ttcagctagt
      481 tgttttaaat cggcgtgctt ttcatcaggc aagatgacaa gatagatttg ttttcctttt
      541 cttgatttca ataatagatt ttttacttgc ggaccaggta gggaaaaagg gacatttttg
      601 actgacgtaa tcgcaggatg atcgactttt tgaaaagaga tacccagttt ttttagtagt
      661 tcataagctt cttgttcggt tgcacgactc atcgcgcatc accctccttt ttttattagt
      721 gtatcataaa aaagagttga caaatgaaaa ataatcatgt aaattacacg taaagttaaa
      781 acaaatgaca tcgaaaagaa gagtagccgt taggtgtttt tagagagtct ctggttggtg
      841 gaaagaggca gcagttaacc gtgaaacaca tcttggagtc gattttctga agcgtatagt
      901 aggaaaatcc ggggagcccg ttacagctgc ggtcgttgct agatgaccag ataaggctgg
      961 cttttccgtg aggagtcggt aaaaaaaggt ggaaccacgt gattaacgtc ctttgatctt
     1021 cggatcaaag gactttttgt tttcatctga acggcaacga ctgtgtgagg ttttttctgc
     1081 aaggaaaaaa taaacagagg tggaaccacg tataatacgt cctcttggca tttgccaaga
     1141 ggattttttt attttaaagg aggaaaagaa gatggagtat ttgattgaag ttatgccgca
     1201 attattagta ggagcactca caacattgaa attattttta tttaccttga ttggctcatt
     1261 accattaggg attgttttag cattaggatt gtctttgcat tttaagccgc tacgctactt
     1321 gcttcaaaca tatgtatggg ttatgcgggg aacaccgctg ttgttgcaac tgatttttat
     1381 tttttatggg ttgccgacga ttgggatcgt ttttgatcgt ttcgaagcag cactcattgc
     1441 ctttatctta aactatgcag cttattttgc agagatcttt cgtggaggga tacaatcgat
     1501 tcctcaaggt caatatgaag cagcgcaagt tttgcaatta agtccctttc aaacggtgac
     1561 gaaaatcgtc atcccacaag tgacaaagat tgttttacct tctgtaggga atgaagttat
     1621 caacttggtc aaagattctt ccttgatcta tatattaggt ttaggtgacg taatgcgtgc
     1681 tgggaaaatc gcgatggaac gcgacgcgac tttactgcct ttgctaggcg tggcaatcat
     1741 ctatcttaca ttgactggta ttctaacaat cgtaatgaaa caagttgaag gaaaattaaa
     1801 ttattaccgc taactgatag gaggaagaaa tatgattgaa ttgaagaaag tgaacaaagc
     1861 atttggtgaa aaaaaaataa tcaaagattt gaatctaatc attccagatg ggaaaacatt
     1921 agctatcgta ggtccttccg gtggcggtaa aaccacgctt ttacgtatct tggctggctt
     1981 agaaagttct gacagcggag aattttattt agacggtgta ccgttcaacc cattcgagct
     2041 taaaatgaaa gaacaagttg taggagtcgt attccaagac ttccagctat ttccacatct
     2101 gaatattttt gaaaacatta ccttagcacc aaaaatggta ttgaaacaga caaaagaaga
     2161 atacaccaaa aaagcagaag aattagcggc ttcattaggg attagtgatt tattgaatat
     2221 ctatccttat cagctttctg gcggacaaaa acagcgggta gcgattgcca gagcattggc
     2281 tatgaatcca cgggtattgg cttatgatga accaacaagt gctttagatc ctgaattacg
     2341 ccagcaagtc gagcgtttga tcctatcatt gaaagaagat ggggtaaccc aaattattgt
     2401 cacccatgat ttagtatttg cagaaaatgt cggtgatcaa ttactaaaag tttccccaat
     2461 tcattaagaa aggagaagat agagatgaaa aaaaccagtt ttctattcgc tttgatcgca
     2521 ggtcttcttc tttttgcagg ctgttcgaac aaaaaaactt cagcagataa ttgggcgact
     2581 atcaacgaag agaaaaaaat cgttataggt ttagacgata catttgttcc tatgggattt
     2641 agagataaag acgggaagct cacaggattt gatattgatt tagccaaagc agtctttgaa
     2701 caatatggga ttacggtaga ctttcagccc attgactggt caatgaagga atttgaactg
     2761 aacaatggaa cgatcgattt gatatggaac ggctattcca aaacgccggc aagagaaaag
     2821 aaagtgcaat ttacaaaacc ttatatggaa aacgatcaag tgctgatcac acctaaagaa
     2881 tcaggaatca caaactttgg acaaatgaat aataagagat taggtgcgca aaatgggtca
     2941 tctggttatg atgtatttac gaaacaacca gaagtattaa aagagatagt caaaaaccaa
     3001 gatgctgttt tgtatgatag ttttaatgaa gcattgatcg atctgaaaag cagacgggtc
     3061 gatggtctgc tgatggataa agtatacgca gactattatt tgaaacaaag aaacgaacta
     3121 gaagacttca acataacaaa aggttcttat gagagtgagg attttgcagt tggtgcacgc
     3181 aagtcagata gtacattagt gaaaaaaatc aatgaagccc tagatcaact agagaaaaca
     3241 ggtgaattcc aaaaaatatc cgataaatgg tttggcgaag atgttacgcc gagaaattaa
     3301 caaatagggg actgtgacaa aagacgtgtc acagtccctt tttccgaata aacagtgttc
     3361 tcctgccaac tccttgtgac tcggagctga atggcaggct cacaacctct tggtaaacgg
     3421 tgttcaaaag ccagtttttc ggtattaagc caaatttatg caaaaatgta gaaagccatt
     3481 ttcacaaaat ttttcttact actcgaaact aagcgacttt ttcacaacct cctttacata
     3541 attttatttt gttgtggaag gggtttcgtg ttattctaaa agaggaacga ttgaaggtta
     3601 taggaaagga agagatgaat ggaacacaaa gaacttacct catttccaaa agattttctg
     3661 tggggctctg cttctgcagc gtatcaagta gaaggagctt ggcaagaaga tgggaaagga
     3721 gaatcagtct gggatcgatt tgtacggata cctggaaaga catttaaagg aacgaacggt
     3781 gatttggcag tagaccacta tcatcgttat aaagaagata ttgctttaat gaaagaacaa
     3841 ggtctgaaag cttaccgctt ctctgtagct tggacacgga tttttcctaa tggacgagga
     3901 gaagtcaatc aagctgggct cgctttttat gagagattga tcgacgaatt gattgaaaac
     3961 gatattgaac ctatgttgac tttatatcat tgggatcttc cacaagcatt gcaagatgaa
     4021 tataatggtt gggaatcacg tcagatcatt gaagatttca ctcattatgc agaaacatta
     4081 tttgaggcat tccgcgggaa agtccattac tggattagtt tgaatgaaca aaatatcttc
     4141 acttcattag gttatttgtt agcagcacat ccgccaggtg tgacagaccc gaaacgaatg
     4201 tatgaagtta accatattgc taatttagca aatgcatctg tcatcaataa atttcacgag
     4261 ttagaaatgc ctggaaaaat cggaccaagc tttgcgtata cgccaaatta tccaattgac
     4321 agtaatcctg aaaatgttct ggctgctgaa aatgcagaag acctgatggc caattattgg
     4381 atggatgtct atatgtgggg aaaatatccg attgctgcca tgaggttctt agaagaaaaa
     4441 ggatgggcac caacgattga agctggcgat gcagaattgc tagaatctgc taaaccagat
     4501 tttcttggga tcaattacta tcaaacggcg acgaatgcat tcaatccatt aaacggagtt
     4561 ggtgctggaa aaatgaatac gacaggaaaa aaaggttcat cagaagaaac tggagttcct
     4621 ggaatgtata aaaaagttga gaatccattt gtcgaacgaa caaattggga ctgggagatt
     4681 gacccagaag gattaaggat cggactgcgt cgtatcacaa gccgttaccg catgccggta
     4741 ctgattacgg aaaatggctt aggagagtat gacaagttga cagaagataa acaaatccat
     4801 gacgactatc gaatcaatta cttacaaagc cacgtcaaag ccatcaaaga agcaatttcg
     4861 gatggagcag aagtcctagg ctattgcacc tggtcttata cagatttgct tagctggctg
     4921 aacggttatc aaaagcgata tggatttgta tatgttgatc aagacgaaac acaagaagga
     4981 tctttggaac gatataagaa agacagttat tactggtacc aaaaactgat tgaagaaaat
     5041 ggagagaacc tttagttcac atacttaaaa caagaaggta cggctggata tggccgtgcc
     5101 ttttcttgat aaaatcattt agaaattaag aaaaactgta aaatgagctg aaaaatgaaa
     5161 gaaagcgtca gttctttcaa acaaaacttt agttccctta cttttttttg tataatgaaa
     5221 cttgatgaaa tgagggataa taaatggcac aaaagaaacg gcctactaaa aaaaggaaaa
     5281 agacgaaaaa aacgaaaaag caaaaacagc agcaggaaca aatgctgacg attggtgttg
     5341 ggtttgcctt gattttattt gctgtcttcg gctttttaaa attaggtttt ttaggaatat
     5401 tgattgcaaa cggctttcgg atcattgcag gaaatactta ccaaatcctc tgtcttttat
     5461 tagccgtatt aggattttgg attgtcatta agaatacaga gttttcaatt ggtaaaaacc
     5521 gacgctggtt tggcggcata ctcttttatt tcggtatctt attattgtta catgcccatt
     5581 tatttggcaa actccatact ggggaaccaa atatcatggg gacgacatgg gatttgttag
     5641 ccagtgatat aaaacaaagt caagtagaca ataacgttgg tggtgggatg attggagcaa
     5701 tcctttatca ttttacttac ttcttgattg cacagcctgg aagttattta gcagcagtgc
     5761 tattactggc aggaggagca ttcctgttca gtaatctgga aggctatcag ctgttaaatg
     5821 gtatccaatc aattggagaa cgagtacagg aattattgga aggtgatcct gccaagcaag
     5881 ctcgaaagca agcggcaaag gaagagagaa tgaaacagcg cgcagaagcg aaagaagcaa
     5941 ggcgcttagc tgcaaaagaa gctgcagaaa aagaagctgc agaatatgag aagaaaaaag
     6001 cggtgagcca gcaaagggaa aatactgctg atgaatggca ggaaccagaa aatacagagc
     6061 cggagcagct aagttttgtt ccaatcgaca gttttcaaga aaatatccat cctgcgaatc
     6121 tagagaaacc agtaccagat acaccaaaac agaccaatac agcagaaggt ttttcagatg
     6181 agttgccaga ggatgacggt acctcattag aatttgagat tgaagcagaa caggaaaatc
     6241 aagactatga attaccgaca gttgatttgc ttgattctat accaactgtg gatcaaagtg
     6301 atgagtataa aaaaattgaa aaaaatatcg gcgtattaga acaaactttc caaagtttcg
     6361 gtgtcgatgc caaggtcgtc aaagccagct taggaccatc cgtcacaaaa ttcgaagtcc
     6421 agccggctgt aggggtaaaa gtaagtaaaa ttgtgaactt aacagatgat atcgcgcttg
     6481 ctttagcggc gaaagatgtc cggatggaag caccgattcc aggtaaatca ctgattggta
     6541 ttgaagtacc aaatggaaag atcagtatgg tttcctttag agaaatcatc gaagcacaac
     6601 caaaccaccc agataaatta ctagaagtac ctttaggacg tgatgtttct ggacgggtac
     6661 aaacggctga tctttcgaag atgcctcact tactcgtggc tggttcgact ggtagtggga
     6721 agtcagtagc aattaatggg attatcacaa gtatattaat gcgtgctaag ccccatgaag
     6781 taaaactgat gatgatcgat ccgaaaatgg ttgaattgaa tatgtacaac ggcatacctc
     6841 atctgttgac gccggtcgtg acgaacccta gaaaagcagc gcaagcctta caaaaagtag
     6901 tacaagaaat ggaagaacga tatgaaaaat tcgcggcaac cggagtaaga aatatttctg
     6961 gttacaatga atttgttcaa cagaaaaact tggaaaacgg aacgaaacat ccaaccttac
     7021 cattcatcgt agtgatcgta gatgaattag ctgatttgat gatggtagcc agcaatgaag
     7081 ttgaagatgc gattattcgt ttagcgcaaa tggctcgggc agcggggatt catatgatcc
     7141 tagctactca acgtccaagt gtggatgtta tcacaggaat catcaaagca aatgtacctt
     7201 ccagaatggc atttgcggta tcaagcggta cagattctcg gacgatcatt gattctaatg
     7261 gggcagaaaa gctcttaggg cgaggagata tgctattctt gccaatgggt gagaataaac
     7321 cgatccgtgt gcaaggagca ttcatttctg accacgaagt agaacgggtc gttcaatttg
     7381 ttacagatca gcaagaagct cattatgaag agaaaatgat gccgacagat gaagttgaaa
     7441 cgacaggtgc tccagaacag ccacaagatg agctgtttga agaagcaaaa gcacttgtcg
     7501 ttgaaatgca aacggcaagt atttcccttc tccaaagaag atttcggatc ggttacaacc
     7561 gagcagcacg tttagtagat gaactggaag ctcatggagt tgttggcccg tcagaaggaa
     7621 gcaagcctcg gaaagtattc attgagcagg aagaaacatc agaaacgttg ccggacgata
     7681 atgacatgta aaacttgtgt gatgaagggt tgaaaaacga ataaccaata aaaagacaag
     7741 gcatgatgat accaaaagaa aaaatggttt ttttccatct tttctaatac ggtatgatgt
     7801 cttgtctttt tagtttttgt gagattaaag ttctattcga tttatttgtg aagtattgca
     7861 ttaaggaaat gaatcatgta tagtatgtag cgaaactttg agaaaaagag ggaaaatact
     7921 ttggaaaaac agtccattta tggcttgacg aatgaagaat taatcaattg gtttatagaa
     7981 aacggcgaga aaaaattccg agctgcccaa gtttgggaat ggttgtatca aaaacgtgta
     8041 agtaatttca cagaaatgac gaatctatcg aaacaattga tcgaaaaatt atcggagcat
     8101 tttatcatca atcctctaaa acagatggtc gtccaagaag cttcggatgg tacagtaaaa
     8161 tatttgtttg aactccctga taaaaacatg attgagactg tactgatgag acaagaatat
     8221 ggcttgtcag tttgtgtgac gacacaagta ggatgtaata tcggctgtac gttttgtgca
     8281 agcgggctgc tcaaaaagaa ccgtgatctg actgctggag aaattgttgc acagatcatg
     8341 atggtccaac attattttga tgagcgcaat cttggcgaac gcgtttcaca tgtcgttgta
     8401 atggggatcg gtgagccatt tgataattac gacaatgtaa tggacttttt gcatatcatc
     8461 aatgatgcca aaggattggc aattggtgct cggcacatta cagtttccac tagtggatta
     8521 gcacacaaaa tcaaggaatt cgctaataac ggcttgcaag tcaatctggc tatttccttg
     8581 cacgcgccga ataatgaggt gcgaacatcg atcatgcgga tcaaccgaag tttcccaatt
     8641 gagaaattga tggaagctgt ggatgagtat ttagagaaaa caaaccgtcg aatcacgttt
     8701 gaatatatca tgttgaatca agtaaatgat agaccagagc atgcgcaaca attagcagat
     8761 ctattaaaag ataagaaaaa attagcatat gtcaacttga ttccatataa tccagtcagt
     8821 gaacatgatc aatattctcg cagtccaaaa gctgatgtat tgagatttta tgatgtattg
     8881 aagaaaaacg gcgtaaactg tgtgatacgt aaagaacatg gtacagatat tgatgcagct
     8941 tgcggacaat tgcgaagcaa gcaaatgaaa aaaacagttg ccaaataatc gtatcctact
     9001 cgttttttca attaccataa aaggagcagt tttcttaatt aaggaactgt tcaacttaaa
     9061 taagagaaaa gattctatag cgaaagctat aaagcttttt ttgacaagaa aaaccctcat
     9121 caagcaaaga aatgtcctca atttgatgac agcctgcttg atgggggttt tagcaattat
     9181 ttaactgatt ttcttatatc cttgctggct ttcttgtgta gcatgataag tttctacgtc
     9241 aagaatggcc tgtttaaatt tttctcgggt gatttcataa ggcacatgtt taatatcatt
     9301 tgttgccatg gctttatcta agattgcttc taaatcggtc gtgacattga gattcatgtc
     9361 ggttaacgat actggaagtt ggttcttttt cataaacaca tagattttat caaacaaatc
     9421 gaactgctga tccatcatca ataaggtcag tacaccataa ctgacagatg ctccgtgaag
     9481 gtatccctcc attttttcta gaacagtcgt accgtaatag aatgagtgtg ccatagcaga
     9541 attatagtta ttgttaacta aaacagaaac caatccggta gtaacaatga tatccgaaac
     9601 gacttgttca attgcatatg agggacgatc gtcggcacta tctttcaatg cttgcgtacc
     9661 ataagtcaag agacgatcaa tgcacatttt tccgatttgg attcctaatc tatccgtgta
     9721 gcctagatca tcgccacgag cagaaaaagt agattcgact tctttggata aagcatcgcc
     9781 aatacctgcc cataagtatt tatcaggtgc ttcggcaata attgctgtat caatgaaggt
     9841 gtgtacaggt ggttttgaag gaaagaagag tccgtccatt acgccttctt cgttatagat
     9901 gacacatacc gcagtgacgg gcgaacagtt cgaaccaata gtgggaaagg tgaagactgg
     9961 tttagctagt ttttcaccaa tgacttttac agtatcgcaa gcacggccac caccgactgc
    10021 aaacaggacg tctgcttttt ggacactttc atgctggatc aactgttcaa tatttccata
    10081 agttgaatta cccccgtacc agagactggc gacaacctct atttcctgtg tcaaagctac
    10141 ttttattttg ttttctgcct ttgaaagtgc agtcttccct ccaatcagca ctacttttgt
    10201 cccatattct ttgacgattc catttgcttt ttcatacgca tctgttccaa tggtataatt
    10261 tggtaagaaa acactttgac tcatattcat ttccttcttt cttcgttgaa acgggttaaa
    10321 attttaaaga gtgatcgatc tgactgcggc tgtagatgga gtagaggtga acctaagaca
    10381 ggataagtga tgaataaggt ggcatgaaca ccttgaaatc atcaataagt atcctaaaaa
    10441 cgaacaagta caaaaacatt gaaaacgaca ataatgaatt tttgttcctc ttagtatttc
    10501 taccgattcc ctttgctttt ttgtaattat ctaaaaattc taaaaattaa gattattata
    10561 tgaaaacagg ttttatttgt caattcataa ttggtttttt ttaaagcaaa agataatcta
    10621 tatcatgatg aacaaacaga gttttttcct ccaattgatg gctttcctga tctttcctat
    10681 tttgaataaa gtgtttctgt ataatttttt attttataag taagataaat aatgaaagaa
    10741 tagagatact gattggtagc taaagatttt tgagcgagaa catttcgttt tttcaaaagg
    10801 actgttagaa taagcaagaa caagggggaa gacgtatgat aatcagtgaa tttgaccgta
    10861 ataatccagt attgaaagat cagctttctg atttactgag actgacttgg ccggaagaat
    10921 atggagacag ctcggcagaa gaagtagaag aaatgatgaa tccagaacga atcgcggtag
    10981 cagcggtaga ccaagatgag ttagtaggat ttattggtgc aatccctcaa tacggtatca
    11041 caggttggga attgcatcca ttagttgtag aaagctcccg acgaaagaac caaataggta
    11101 ctcgattagt caattactta gaaaaagaag tagcttccag aggaggaatc acgatttatt
    11161 taggtacgga tgatttagac catggaacaa cgttaagtca aacggacctg tatgagcata
    11221 catttgataa agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa
    11281 aattaggtta taaaatcgta ggtgtcttac caaatgcaaa tggctgggac aaaccggata
    11341 tttggatggc aaaaacgatt attcctcgac caaattctca ataataaaaa gaggttgtga
    11401 cagaagtaat cagcttcaag aaataagaag gaatttacga aaattacttc ttgaattttt
    11461 gtgaaattcc aacttatttc cgaagaagct acttttggaa caccgtgtac c
//
LOCUS       DAYYGO010000091        11385 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000091 DAYYGO010000000
VERSION     DAYYGO010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.093"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..76
                     /locus_tag="ACHA5T_002101"
     CDS             <1..76
                     /locus_tag="ACHA5T_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="head-tail adaptor protein"
                     /protein_id="HFM8887659.1"
                     /translation="NVIYADYDNFYIYFYLESVGEYGD"
     gene            66..467
                     /locus_tag="ACHA5T_002102"
     CDS             66..467
                     /locus_tag="ACHA5T_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887660.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            460..864
                     /locus_tag="ACHA5T_002103"
     CDS             460..864
                     /locus_tag="ACHA5T_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887661.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            876..1481
                     /locus_tag="ACHA5T_002104"
     CDS             876..1481
                     /locus_tag="ACHA5T_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8887662.1"
                     /translation="MAKTTTVTTFDNISIKRISFNFKNATNAISTDCNGQLDGETEMQ
                     RIVKKCGSTEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDERIKSGIYSYGPDSVG
                     EDFSLAAEIVDDFEEKNKLIGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFGK
                     FYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            1501..1863
                     /locus_tag="ACHA5T_002105"
                     /pseudo
     CDS             1501..1863
                     /locus_tag="ACHA5T_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1866..2048
                     /locus_tag="ACHA5T_002106"
     CDS             1866..2048
                     /locus_tag="ACHA5T_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887663.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            2066..5485
                     /locus_tag="ACHA5T_002107"
     CDS             2066..5485
                     /locus_tag="ACHA5T_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFM8887664.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASEAEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLSKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQTFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISGIFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGEAGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKIQNDNASAWR"
     gene            5536..6273
                     /locus_tag="ACHA5T_002108"
     CDS             5536..6273
                     /locus_tag="ACHA5T_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8887665.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            6283..8574
                     /locus_tag="ACHA5T_002109"
     CDS             6283..8574
                     /locus_tag="ACHA5T_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFM8887666.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYDTAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNKPFLSFGAPNFR
                     YSNASYITDGDGSSLSINGSAGSSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKNFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDVRLKEDTAYDSTVLDQKRF"
     gene            8598..10679
                     /locus_tag="ACHA5T_002110"
     CDS             8598..10679
                     /locus_tag="ACHA5T_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887667.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSITSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFLAEIKGRMDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLHVMYSNSIDFGGYDYSGNPNLMSKLKSSDFN
                     VGYHGSLTNVNGMLHFMSDGTGDIDMFTRINTPQLASGKTYTLSAKVRFDEGTTGAID
                     KLRLVYRTSPGEKILLEANSTNITTDDVGKEITIKGTANVNYQITNLDRFYISVSFSN
                     RDKINGGFKLYDIKIEEGSTATPYQPNLLDDPYWVGKTSLGKNIANKGTTFPIKSSAY
                     EIYKGNTEEELIIGQTYTITLKGTKPASQTFVAYNYWNVNFGDLKPVEGLTDVWSLTF
                     TPTKLEPGLPKDLRIFQSPKETAGACRIDWLKIEKGDTRTPNIEQYKYRGIGMRDSNN
                     PKDYVWDLEPSYVENNLATDIKVAEITGKANNYTDGKVSEINSQLTASINEVDTTAKD
                     AQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALN
                     GKRSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYG
                     SDYTFAITYTI"
     gene            10696..10845
                     /locus_tag="ACHA5T_002111"
     CDS             10696..10845
                     /locus_tag="ACHA5T_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887668.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            10842..11288
                     /locus_tag="ACHA5T_002112"
     CDS             10842..11288
                     /locus_tag="ACHA5T_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887669.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            11290..>11385
                     /locus_tag="ACHA5T_002113"
     CDS             11290..>11385
                     /locus_tag="ACHA5T_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFM8887670.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEE"
BASE COUNT         4015 a         1749 c         2403 g         3218 t
ORIGIN      
        1 caacgttatc tatgccgatt acgataattt ttatatctat ttttatcttg aaagtgtggg
       61 tgaatatggt gattgataat tctaaagaaa aagaacgttt aaataagcaa atttctgcta
      121 tcaaaacttc cttagaagag cattttggcc tcaaactctt tcaagactcc gtaggcgagg
      181 atgagctacc tgatgatttt aattacttca ttctcgaaac aggagaaata gaaatgatca
      241 ctgagccaaa atatagcgtg ggtcaaaatc tatatctaac tttctattca gaaaatagag
      301 aagatttaac aggagattca ctagatatta tttcattgat tcaaaatcgt tcgattcgtt
      361 ttcagagaat ggatcccaat catttaaaac tagagaacca agatcgctat atcgatcaat
      421 tggtatttac gtttagacga ttattgaaga gtgattgtca tggctaaaaa tagttgggag
      481 ctaaaaataa atggacatga tgaacttctt gtgcggatgg aacgctattc aagcgagagc
      541 gaacgactga ttaacgaagc attgaaatca aaaggttcgg ctattgcagt ggataggatt
      601 acagaaaaaa ttcctgtttc tgaagcagat ttaagaagag ggcaccaaca cgcaaaaaat
      661 agtcgtccac ttaagactca atacattaat ttgggtttca tcattagacc tacaagaaaa
      721 tttgagtatt taaaatatcc tgatttgggg ataggtactt ctaaaagaaa tcagccggac
      781 gaatttatga gaagaggatt aggtcttgca cttgatccaa ttacagaact tctgattcgt
      841 caattcgata aattaaataa ataggaggaa caacaatggc taaaacaaca acagtaacaa
      901 cttttgacaa tataagtatt aaaagaattt cttttaattt taagaatgca acaaatgcaa
      961 tttcaactga ttgtaatgga caattagatg gcgaaacaga aatgcagaga attgtaaaaa
     1021 aatgcggttc aacagaagta aaatcgaaat ctaaaccaat caatatgacg gtaacaatta
     1081 ctgcacatgt accgatggaa gtttatcgac gcttcaatgg gttgaaacaa gatgaacgta
     1141 ttaaatcggg aatttactct tatggtcctg attccgtagg ggaagatttc tctcttgctg
     1201 cagagatcgt ggatgatttc gaagaaaaaa ataagctgat tggtatgtta gcatgcactt
     1261 cgaatacagg attaacattc tctattgaaa atggtgcgga tgaagtagct gcgttagaac
     1321 tagaaacaaa agttatgcaa gatgaatttg gtaaatttta tcatgaagca attgttgcag
     1381 aacttgaaga agacttaaca gatcaatgga tgacgaatct atctgctgat gtgattaaaa
     1441 aaacttcagt tgtgacaact acggccactc aatcacagta aaaaaaacgg aggtagcgaa
     1501 atgaacgaag attactcaaa aattgaacta aacgatggaa caattttgaa tttagaacct
     1561 aaactgaata tcaagaaatt attgatgatc aatagagatt ttaacacaga cgagtttgca
     1621 aaaatgtcga tgggaaaagg ctctatggat attacagtta ttcaaggtgc aaaagccgta
     1681 tatgtagctt atcgtcaagc gaacatggtc gattacattt cattcgatga atttatagat
     1741 aaatgagatt ttgatatgga ggttgcagta gctgtataca gtactatgat gttcaaacaa
     1801 gcacgtgatg cctatcaaaa agaattcgaa aaagcaaata aggaaaaaaa gcttcaaaag
     1861 taaaaatgcc aaagctctta gttgaaacgt gggtcgatgt ctattcgatg ttgaccgacg
     1921 ttttttctat gccttcagat ttggttttaa gcgatatctg tttagatgac attttgcaaa
     1981 tggcttacaa caagagcgct tatgaaggat ggaagaacta tgcaataaat caatcccaga
     2041 aaaactaaag gaaaggaggt aaaaaatggc taaaaagaga acggaagcag aagtaacttt
     2101 catagctaac gatgacggat tgaaatctac gttaaaagaa atcagtgctg aattaactaa
     2161 aaatagagca gaattaaaac tagaacaagc tcaattgcaa cagactggtt ctgaatcaga
     2221 caagttagga agtaaattat cttctttaga aaagcagtat gaattacaaa gtcaaaaagt
     2281 tgaagtaact agccaacgtt tagccaatgc gaaaaaatat tatggagaaa attccaccga
     2341 agttcagaaa cttgaaagag aactgattaa tcaacaaaca gcgcaacaac gtttgtcaaa
     2401 cgaaattgat aaaacgagta atgcactagc tcaagcaaaa ggcgaaatac agacgtacga
     2461 gtctacaatg caacagttgg atagcgaaca aaaaaatgtt caagctagtg cttctctgat
     2521 tgaatccgaa tacaaaaaat ggcaagcaac tgctggtcaa tcagcttctg aagccgagaa
     2581 attagcgaaa gcccaagaat atgtttctca acaatctgaa aatgcggaga aaacgataga
     2641 tatcctaaga cgacagttag aagctacaca gtctgaattt ggcgctacat ccacagaagc
     2701 aatgcagatg gaagcgaagc ttaatgatgc tgaacgtgaa tttgaagagt taggacaagc
     2761 tgctaaaaat gtagatacaa ctaacttgga cgatatcgga agcaaaatag atatgaataa
     2821 tttaatggaa gcttctgacg ttttaagcga cattggcgat aagcttacag aattagggaa
     2881 acaagcagtg gactctgcta acagtgtagg tagttcccag agtaagatac aagctaattt
     2941 tggtttgtct aaacaagagg ctgaagaatt aacgaatgta gccagagaca tttattataa
     3001 aggttttgga gaatcgttag atcagtccac agatgcattg attttggtaa agcgtaattt
     3061 aggagattta aataatcaag atttacaaaa tatcacggaa caagctatgg tcctagaaaa
     3121 caccatgggc gctgatatgg atgaaacgtt acgtggtgta aatggcttaa tggtcaattt
     3181 cggcttgagt gctcaagatg caatggattt aatggtttcg ggtactcaaa acggtttaga
     3241 taaaacgcac gaattaggcg acaatatggc agaatatagc caattatgga gtcaaatggg
     3301 atattcagct gatgaaacgt tcggaatgct tcaaaatggt ttagatgcgg gtgcttataa
     3361 ccttgataaa gtcaatgact tagttaagga aatgggaata tcgttaacag atggtcgatt
     3421 tgagcaaaac atggatatgt ttagtgaaag tactagaaaa gcttttgaag agtggaaaaa
     3481 tggcggagga acacaaaaag acgttattaa ttccatgatt caagatttta gcaatatgga
     3541 tggtcaatac gaccaattaa ataaagcttc gacaatttgg tctgcgcttg gcgaagataa
     3601 tgcgatgaaa gttgtccaat ctttaactga tgttaaccat acatttgatg atgttagtgg
     3661 atctgcacaa aaaatgaatg aagattctac tactccgtta caagaattaa acggaaaaat
     3721 agctgaatta aaggattcat tagctcctat aggcaacaca atcatagatg cactcgaacc
     3781 agtaattgat tttctaggaa agatggctga tgcgtttaat aatcttccac aaccagtaca
     3841 ggattatgcc gtagcgattg gcggattgac tgctgcattt actttattaa tgccaataat
     3901 agttggcttc atggctctag gtggtcctac tacattaata ataggagcag ttattactgc
     3961 tattgctgga gttatagcaa ttataaaaaa ctggggcgca attactgact ggtttaaggg
     4021 aatatggagt aaattcactg attggttggg tggtacttgg gaaagtataa aagaaggtgc
     4081 ctcatcagtt tgggatggag ttaaagaaac ctggtctgga tttgtagatt gggttcaaga
     4141 tatttggcaa ggagtttctg attggtttgg agagctatgg agcggattag ttgaaggagc
     4201 ttccaacatc tggcaaggag tccaagaaac ttggcaaaca ttcgttgatt gggtttcaaa
     4261 tatttggaac ggagtcaaag aagtatggtc gattatttgg gcagacattg taggaattgt
     4321 tcaaatacca tggacattaa taacgtcatt gattcaagct ggtattaata ttatcgtggg
     4381 tatttttgat gtagctggac agttattagg cgcagcttgg caagctgttt ggacacctat
     4441 ttctgatttc cttaaaaata cttgggatac tatgacacaa tggataagca tcgcttggaa
     4501 cggaattgta actacattcc atactatatt tgatccagta gtggcatggt ggaatggtat
     4561 atggacatct attagtacta cggcttcaaa tatttggaat tcaattagtg caacagcttc
     4621 tagtatttgg aacagtatca agaatacaat cactagcttg gtacaagcag ctgctacagt
     4681 aattcaaaat atttggtcaa ctgtatctag ttggttaggt ggaatttgga attcaatcag
     4741 ctctacagca tcaaatatct ggaatagtgt gactagtagt ataagcaatg ctataaacgc
     4801 agctaaaagt gccattcaaa gtgtttggaa tagtatatct tcgtggatca gcggaatttg
     4861 gaacggtatc aaaaacactg ctttgaatct ttggaatgga attacaagca ctattagctc
     4921 taaagtaaac gatggaaaaa atgcaatttc aagcggttgg tccaatctaa caggtattgt
     4981 ttccgacata ttcaataatg ttaaaagtac aattgctaac atttgggaag gtatcaaaaa
     5041 gactgttagc gctccgattg attggattag agataaaatc agtggcatct ttgataattt
     5101 gaatatttcg ataccacata ttccgttacc acattttaaa ttgagtgggg aattcaatcc
     5161 attgaaggga aaaatcccaa cgttgggtgt tgattggtat gcgaaaggta gtgtgtttaa
     5221 ttctccgaat attatcggtg tcggtgaagc aggacctgaa gcagttttac ctttgaaaag
     5281 atctgttttg caagaaattg gtgatcgtat cttgagtagc acatcagttt catctagggc
     5341 acaaacgatt caacctgtga ataactacga attcaatttc acaattgatg gtaacgcaga
     5401 tgaggttact atgaagcaaa caactcaaca aatcattgat agcattacaa aaattcaaaa
     5461 tgataatgct tcggcatggc gttaaacagg agagtatttc tcctgttttt ttagtattaa
     5521 aaaggatgtg aaaaaatgac tgattgtata cattctataa tcgatggatt tcctgattat
     5581 ttgcataaat tggctttagc tgaaagacca accatacctt ctccaaaaag acagagagtt
     5641 gaaacttctg ttttaggtag gttaggtggc ttagtacaag attactcgtt tgaagatatg
     5701 tcgtttacat tgcactataa ctatttagag gatgtggaag accatcaagc gttcaagcaa
     5761 tcgttttata tcatgcgtca ttggttaaac tatgcaaaga aattagaatt ctctgacgat
     5821 cccaacgtct attacgttat ccagactatc gatattgggg atgcagaaaa cgatattgtt
     5881 gaatggggag agttcgatgt aaatattact gcgaaaccat tcgcaagagt tcaagaagat
     5941 gtacctataa ccgtagataa accacagtca tttaacttgc tgaataatag tttagaagaa
     6001 agttttccaa agattatcat cactccttca gctacttcat gccagttcat cttaaatgat
     6061 tatgtgttta gtttcgaagg cttagtagca ggaactgatg tagtcattga tagtgatttg
     6121 atgctttgct acgaagagca atcggacgga gatattttag atcggtccaa caaaatgaag
     6181 accatgcaat atccaacgtt gcaagtggat attaatcatt ttaattgtac tggtttgagc
     6241 aaaatacaaa tttatcgtaa cgggttaagg taggtgaaat agatgatcga taatttaata
     6301 actatttacg ataaaaatga cgcgaataat ttagctgaac atttatatga tacgcaaggt
     6361 ttaggcgctt tgtcagactg gttaacagct actgttagca ataaactaaa cggagccgag
     6421 atatttcagg gtacttattc aataaacgga actaatgcag atttgattgt agaaggacgt
     6481 attattcagt gttatgtaga tgaaaatcga gcaaaacagc gtctacggat ttattatgca
     6541 aaaacttctg taataggcaa tacgatagaa gtaaaagctg aacctatttt caatgatata
     6601 agaaaatcgg tgttgaataa atatgacagc ggaacagaaa agatcactgc tactcaggca
     6661 tggcaaaacg caaaagtttt agcgaaacca gctatccctt cgcagttttc tttctcgtca
     6721 ttagtagata cgcttgctaa tgtgaagata gaaaaggcga attttttaga attctttggt
     6781 ggaaaagagg gatctattct agatcgattt catggtgagt ttctaaaaga taataacaca
     6841 ttacgtcatg aaaaaagtct aggtacggat cataaaatca aagcgattta tactaaaaac
     6901 ttaactggtc ttgacttaga gattgatgct caaagtgttt tagttggagt ttatccattc
     6961 attagcagct cttcagaagg agaagacgag atcactctac cagaagaagt tattttcacg
     7021 gattacgtgg atgattatcc tgctggatat gtttcttttg ttgattttaa agacaaagcg
     7081 actgatgtag ccacattaag ggaagctgct aaagactggt tgaaaacaaa catagataaa
     7141 caaaaaccac aagtgagtgg ttcgattgaa ttagtaccat tgaggcatca aagaggctat
     7201 gaaaaatttg ttgatctaga aaaagtttcg atgggtgacg gagtagatgt gtatcatcca
     7261 cagttaaaag tgaatatgtc agcgagaatt gtggaatata cgtttaatgt tctaaccaat
     7321 tcatacgata aattagttgt aggaaacgtc aaaacaaact tcttagaaaa cacagagaat
     7381 aatgtcagca atttgattaa tgatgccatt gatcaattga aaaatggtgg cgaaatcagt
     7441 gatttaatca atgatattgt agatcatcaa actgatatga ttactggtca aaatggtggt
     7501 tacgttttat tagatcctaa agaagcccct agtcgtattt tgattatgga cacaccagat
     7561 aagaatactg cacggaacgt tttgcaaatc aacaacgctg gtattggttt ctctaaaact
     7621 ggcattaatg gaacgtatga caccgcatgg acgttagatg gcggattcaa tgcctcgttt
     7681 attacagctg gtgagatagt agggattact attagaggta ctacattaat tagtgatggc
     7741 actgattata gaacaagtat agctaatggc aaaatgactt ggtactcaaa aaaagttaac
     7801 aaagatatta tggagctaga agcacgtgat tatgtaagtg ctgatgcagg tattgtatca
     7861 tacaccatga aaactggtgg tggtttcatg attagaaatc cacaggggaa tttggttttt
     7921 agtacgtggg ataatggtaa taacaaaccg tttttatctt ttggtgcgcc caatttcagg
     7981 tatagcaatg ctagttatat aactgatggc gatggtagtt ctttaagcat taatggtagt
     8041 gcgggcagtt catgggagtt taaagtagct ggtaggacta tgaaatttac tagtgatggt
     8101 atgctaacgt tgccaggttg tttttttggt tcatgggaag atggaaaact tgctaggttt
     8161 gaacaatcaa cggtacaagt atataaaaat tttactgtta gaggtactaa aaactcaacc
     8221 gtaccaacag aacattatgg acaacgacta ttaaacgctt atgaaactcc agaatattat
     8281 ttcgctgatt atggggaagc cgttacaggt gatgatggta aagttcgtgt tgatattgat
     8341 cctatgtttg ccgagacagt aaatctaagt cgatatatga cacatgtgac acctacagag
     8401 ctagttttgt gtgctgttac tcatgaagat attgaccatt tcatcattga aactagtaag
     8461 ccaaacgtat tggttagatg gaatttagtg gcacatcgac tagggtatga agatgttaga
     8521 ttaaaagagg atacagcata tgatagcaca gtgcttgacc aaaaacgttt ttaaaacgaa
     8581 gacaaggagg tatataaatg gctagcagtt tatataattt ggctttagat ttcagcaaag
     8641 aattaaacta caccaaagct attatggctc gtcaaggtga caaaggaatt acggtgactg
     8701 ttaaaccgtt tttaaatggc ttgcagatgg atacgagtgg cggaaccttt acattaaaag
     8761 gaacaacacc atctaaccgt tacgtagata gtgtcgctac tagtataact agcgaagaag
     8821 tcacattttc tcttgatgga acatttatga gtgaagcagg atactataaa cactgctacg
     8881 tagaatatag aaaagataat caaattttaa cgacgcaaga tatcattttt ttctcactag
     8941 gagtgtctga catttcgcaa ggccaagccg atgaatatgt ttcgcaatta gaagaattga
     9001 ttcgaaagta taacgaaact tttgatgctt ttctggctga aattaaaggt agaatggata
     9061 gcttaaataa acagattact gatttaactg gtcaagctaa aacactacaa gacaagttag
     9121 atgctctgaa agaagaaatt tctaaattag gtaacttaca tgtgatgtac tccaacagca
     9181 tcgacttcgg gggctatgat tatagtggga atccgaattt aatgtccaaa ctaaaatcga
     9241 gcgattttaa cgttggttac cacgggtcac taactaatgt taacggaatg ttacatttta
     9301 tgtctgatgg tacaggggac attgatatgt ttacgcgtat taatacacct cagcttgcta
     9361 gtgggaaaac ctatactctg agtgcgaaag ttcgatttga tgaaggaact acaggagcta
     9421 ttgataaatt acgtttggtg tatcgtacat caccaggaga aaagatatta ttggaagcaa
     9481 atagtacaaa tattacaaca gatgatgtag ggaaagaaat aacaatcaaa ggtacagcta
     9541 acgttaatta tcaaatcaca aatttagatc gattttatat tagtgttagt ttttctaaca
     9601 gggataaaat aaatggtgga tttaaattgt acgacatcaa aatcgaagaa ggctcaacag
     9661 ccactccgta tcagccaaac ttactagatg acccttactg ggtaggtaaa acatcgttgg
     9721 gtaagaatat tgcaaataaa ggtacgacgt tcccaattaa atctagcgcg tacgaaatat
     9781 ataaaggtaa cacggaagaa gagcttataa taggtcaaac gtatactatc acgcttaaag
     9841 gaacaaaacc cgcaagtcaa acctttgtag catataatta ttggaacgtt aattttggag
     9901 atctaaaacc agttgaggga ttgacagacg tatggtctct aacattcaca ccaacgaaac
     9961 ttgaaccggg tttgcctaaa gaccttcgca tttttcagtc gcctaaagaa acggccggcg
    10021 catgccgaat tgactggctc aagatcgaaa aaggcgacac acgaaccccg aatattgagc
    10081 aatataaata ccgaggaatc ggcatgcgag actcaaacaa tccaaaagat tatgtatggg
    10141 atctagaacc tagctatgtt gagaataacc tagctacaga tatcaaggtt gctgaaatca
    10201 caggtaaagc aaacaattat accgatggga aagtatcgga gattaattcg cagttgactg
    10261 cttcaattaa tgaagtagac accacagcta aggatgctca aacaaaagcg aatgctaatg
    10321 cgactgctat agatgaatta gacaataaga tcgatgaacg cattaatgat acagctacta
    10381 ccacattaac agttacaaac gggaataccg gatcagcaaa gctttatcgt gaaggaaaaa
    10441 cagtttctat atattttgtg gctttaaacg gaaaacgcag tggtggaaat gattcaacga
    10501 tactaacaat tccagaaggc tatcggccac caattagttt tgagcaactg gttggctcga
    10561 tagaccgttc tactttgaac agtgctcagt tatctattgg tgcagatgga gccattaaat
    10621 ggcgaagaaa ctcaagttat ggatcggatt atacctttgc aattacttac acgatttaga
    10681 aaagcgtgaa tcgatatgaa ggcagcatat agaccaattg aaccttacgg attcgagcaa
    10741 atcattgtga atgatgaaga acatttaccg gaagaatgca cagaagtcga accaccgatt
    10801 ccaaattgga aaccgaaatt caattactgg gagggaaata aatgaaaaac atttggaaat
    10861 atggacgtac tggcggagag tacgcaggaa aagtattgga cgacatgctt gtatccgttc
    10921 cttacacaga tcagccaccg cttgaaggaa ttcgtgctga tggcgaaccg ttaacaatcg
    10981 ctgatcaaat gtttgatcct aaattgaatc aatggattat tttagcgaac gcactagatc
    11041 acaacgattt aaacaatctc aaagcaatgt atgagtcgtt agaaaatgag aacggcgatt
    11101 taaaacagat caatgccaaa ctcatgctaa gcgatgtagc aattaaacag gaaaatactg
    11161 cattgaaaga aaaagcggat agtttagcac aaatcaattc aaaaatgatg cttgcttcgt
    11221 tacaaaatag caaagacatt tcagaaatta aagagcaact aaatccagct tcaaagggag
    11281 gtgagtagta tgtttagttt tagcgatgtg aaaatgatgt atgattgggg ctgtttcact
    11341 aacgaacaag taatggtttt cgttccgttg tgcattactg aagaa
//
LOCUS       DAYYGO010000092        11163 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000092 DAYYGO010000000
VERSION     DAYYGO010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11163
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.094"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            125..1384
                     /gene="lysA"
                     /locus_tag="ACHA5T_002114"
     CDS             125..1384
                     /gene="lysA"
                     /locus_tag="ACHA5T_002114"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFM8887671.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1371..2171
                     /gene="dapF"
                     /locus_tag="ACHA5T_002115"
     CDS             1371..2171
                     /gene="dapF"
                     /locus_tag="ACHA5T_002115"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFM8887672.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2174..3055
                     /gene="dapA"
                     /locus_tag="ACHA5T_002116"
     CDS             2174..3055
                     /gene="dapA"
                     /locus_tag="ACHA5T_002116"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFM8887673.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3052..3768
                     /gene="dapB"
                     /locus_tag="ACHA5T_002117"
     CDS             3052..3768
                     /gene="dapB"
                     /locus_tag="ACHA5T_002117"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFM8887674.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3774..4469
                     /gene="dapD"
                     /locus_tag="ACHA5T_002118"
     CDS             3774..4469
                     /gene="dapD"
                     /locus_tag="ACHA5T_002118"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFM8887675.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4485..5486
                     /locus_tag="ACHA5T_002119"
     CDS             4485..5486
                     /locus_tag="ACHA5T_002119"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFM8887676.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5483..6844
                     /locus_tag="ACHA5T_002120"
     CDS             5483..6844
                     /locus_tag="ACHA5T_002120"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFM8887677.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6804..7955
                     /locus_tag="ACHA5T_002121"
     CDS             6804..7955
                     /locus_tag="ACHA5T_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM8887678.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7930..9099
                     /locus_tag="ACHA5T_002122"
     CDS             7930..9099
                     /locus_tag="ACHA5T_002122"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM8887679.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9274..10653
                     /locus_tag="ACHA5T_002123"
     CDS             9274..10653
                     /locus_tag="ACHA5T_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8887680.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10934..11163
                     /locus_tag="ACHA5T_002124"
                     /pseudo
     CDS             10934..11163
                     /locus_tag="ACHA5T_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3720 a         1949 c         2497 g         2997 t
ORIGIN      
        1 aaatatttcg aaatatacta gacaattcag aaaattttgt ttaatattat tccaataaaa
       61 ttcaaatctt tatcaaatga aattccaata aaaacggaaa aaaagtgaaa agaggaatga
      121 aaaaatgaca gaattgataa ttggcggagt cacagcaagc acattggcag aaaagtataa
      181 gacacctctg tatgtgtatg atgaagaaaa gatgagacaa acgatgctgt cattcaagcg
      241 aggctttgta tcgaaacgtt ttgatacaaa agtgctttac gcctctaaag cattccaaac
      301 gattgagatg ctgcatttag ttcgtgaata tggattcggg ctagatgttg tcagtggtgg
      361 ggaaatctat accgctttgc aagctgattt tcctagagat actatctact tccatggtaa
      421 caacaagacg ccagaggaac tgatctatgc acttgaaaat gatttgttgc atattgttgc
      481 cgataatttg atggaagttg aattattagc agaattaagc caacgctatt taaaaaaaat
      541 ctgtatcatg cttcggttga atgtcggagt agaagcacat actcatgaat atatcgtcac
      601 tgcgcatatt gattcaaaat tcggcatgag ctatgaaagt aaagagtgcc aacaagccct
      661 tcagttgata aaagaaagca gctatctaga gttagaagga tttcatgctc atatcgggtc
      721 acaaattttt gatgttactg cttggttagc tgaaatcgac aaattgacga attacttaac
      781 cgattttgac caacctcttt ccttgaatct tggcggaggg tttgggattc gttatacaca
      841 aaatgatcag ccgatcccag tcgaagaggc actaaaaaaa ctgattgaat atacggaaga
      901 agctttggaa aaacgaaaac tgaccattcg tcaattactg atcgaaccgg gtagaagtat
      961 tgttggagaa gctggtacaa cactatacac gattggtttc atcaaaaaga caccacataa
     1021 aaaatattac tttgtagatg gcgggatgac agataatatc cgtccagctc tgtatcaggc
     1081 agaatacgat tgtgatttag cagcaaaact gtcggaagaa aaaacagaga aagtgacgat
     1141 tgcaggaaaa atgtgtgaat caggcgatgt ggtaattaaa gaagcttatt tgccgaaagc
     1201 agaatcgggt gatcttttag ctgtttattc cacgggagct tatggatact cgatgagcag
     1261 caactacaat cgagcgacac gaccagcagt tgtttttgtc aataatggcc aatcaagact
     1321 gattgttcgc agacaagcat tttctgatct actaaggggg gaagtttcct atgatccttc
     1381 ataaatataa tggctgcgga aatgatttta tcttattaga ttacgcagaa gacacagatt
     1441 atagtcaatt agccgcttct ttatgcacaa aagaaaaata tgatatagac ggattgatcg
     1501 ccgtaaaaat cgatccgttg gaaatgatct attacaacaa agatggcagc cgcgcgccga
     1561 tgtgcggaaa tggcattcgc tgttttgctc gatacgtaaa tgatcagaac atgatgaatg
     1621 aacaggaatt caacgtagag acattagcag gcacaagaaa aattacggtg ttggatccct
     1681 caccttttta ttgcagcgta gatatgggag tgccagatga ttctccggaa aaaatcaaag
     1741 ctaatcaaac ttatccaata aaaaaccagg aaatagagat caatggtgaa aaagtaacga
     1801 tcactagttt atttttagga acgattcata ccgttgtttt tgttgaaaat gcgcgtgagg
     1861 aagtctggag aaaaagaggt gaactgatct gtcatcatcc tttgttcaaa gaaaaaacca
     1921 atgtcaactt tgtagaagta gtcaatgaac aagaattgat cgtccgtacc tatgaaagag
     1981 gagtaggttg gacacttgct tgcggtacag gttgctgtgc ctcttatgtg gttgctaagg
     2041 aaactgggaa aatttcagca gatcaggtag ttgttcactt ggaacaagga gacttgatga
     2101 tctctggtga ggatgcgata caaatggctg gcccagcagt tcatgaatgg acaagaatat
     2161 tggaggaatg aagatgataa aaggttcgat cgtggcgctg atcacgccga tgaatgaaga
     2221 gggatcagta gattatgctg gtttggaaaa attgatccaa ttccatttag atgaacaaac
     2281 agatggatta ttagttttag gtacaacagg agaatcttct acgctcacac aatctgagga
     2341 ggagcagatc cttcaactga cagtaaagaa agtggctggc cgtgtgccgg tgattgcagg
     2401 agctggaacg aataatacga aagaaacgat tgaaaaagcg aagcattttg cttctttggg
     2461 ggcagacgca cttttggtca tcacccctta ctacaacaaa acaagtgatg caggactagc
     2521 tgctcatttt acagcaattg cagaagcctc accgattccg cttattttgt ataatgttcc
     2581 ttctagaaca ggcatgtcga ttccaatcca tgtattagta aatttagcag aacatcctaa
     2641 tatcattggt ttgaaagaag cctcaggaga tatggcttat gtgatggatg ccgctcggtt
     2701 gattggagaa gaattcttcc tttacagtgg aaatgatgat ttgatcctac cagtaatgag
     2761 tgtaggagga agcggtgtaa taagtgtatg ggcgaatatc caacctaaaa tagtccatga
     2821 attagtaaaa gatacgcagg acggaagatg gcagcaagca aaagaaaagc aattaaatgc
     2881 acttgaactg atccatgcct tgttcagtga gaccaatcct attcctgtca aagcagccat
     2941 gtcattatta gacttacctt ccggacctct acgcctgcct ttagtttctc tttcaaaaga
     3001 gaaaaagaaa caattagctc agcttttgtt aaaagaaagg acggttgaaa aatgaaaatc
     3061 ggactgattg gcagtggaca gatgggaaat agagtcgaag aagttgcaca agaaagagga
     3121 gatacggtcc ttcttttacg gacggcacct aaagaagcaa ctacgcttgc ttttacggaa
     3181 ctgccagaac tgcttattga tttttctcat ccagataatt tagagaaaat cctttcgtat
     3241 agcttatcat atcaacttcc gttagtcatt ggcacgacag gttatacaca atcacagttc
     3301 caagaaataa aagaacatgc caaacatgta cctgtgatgt atagtgctaa tttttcgttc
     3361 ggcatcatgg tgatgaatca actaatcaag caagcagctg caatgttgca aggctggcag
     3421 atcgaactga ttgaaaaaca tcatagtcgt aaaaaagatg caccatctgg tactgcaaat
     3481 atgctgcttc aaacaatcca agaagtgcaa aagctgcagc ccgtatacaa ctggcaaggg
     3541 aaaaaaagag aagaaaacca aattggtgta cacagtattc gtgcaggttc tttacctggt
     3601 gaacatgatg tattgtttgc tgctactgat gaaatattta cgatcaaaca tgaatctttt
     3661 tctaatcgaa tctttgctgc aggcgcagta gaagcagcgg actggttgaa agatcagtca
     3721 ccaggttatt acaaattaga agatttatta atggacaaag gggtgtaaaa aacgtggaag
     3781 ctcaagaaat catccgctac attcaaacgg catctaaaaa gacgcctgtg aaagtctatc
     3841 tgaatgtaac cgagccaatc gaatttaaag aaagtaaagt ttttggtgaa ggaagcagct
     3901 tcgttgtttt tggtgactat gagacgattg ctccggtttt agaagctgaa tcagaaaaaa
     3961 tcattgaaat cgaaatcgaa actgctgcaa gatacagcgc agttcctttg ctagatatta
     4021 aaaaaattaa tgctcgtatc gaaccaggtg ctattatcag agatcaagtg tcaatcggca
     4081 acaatgccgt gatcatgatg ggagcgatta tcaatattgg tgcagtaatc ggtgaaaaca
     4141 cgatgattga tatgggtgct gtcttaggtg gacgagcgac agtgggcaaa aactgccata
     4201 ttggtgcagg agctgtctta gcaggtgtga tcgaaccagc atcggccaaa ccggttatcg
     4261 tagaagacgg cgtactggta ggtgcgaatg cagtgattgt tgaaggtgtc catatcggaa
     4321 aagatgcagt cgtggcagca ggagcagtgg ttttagaaga tgtggcagca gaaactgttg
     4381 tcggtggaat acctgcacgt gtattaaaaa taagtgatga tcagacaaaa gaaaatacag
     4441 cattgattgc tgcgttacgc gaattataga ggagatggag aaaaatgcgc gaaatagata
     4501 cctgtattgt gggggcaacc ggattagtcg gccaaacgat gctcaaagtg ttagaagaat
     4561 atgattttcc agtaggacga ttgaaattat tagcttcaaa acgttcagcc ggtaaagttt
     4621 gtgtattcaa gaaaaagaaa cacactgtac aggaattgac agaaacttca tttgaaggat
     4681 atgatttagc gctattttct gcaggtgctt ctgtatccaa agagtttgcg ccaatcgcac
     4741 gaagtcaggg actatttgtc atagataatt cctcagcatg gcgggaagat tcggatattc
     4801 cattaatcgt tccagaggtc aatctggaag actatacatt gaatcggctg attgcaaatc
     4861 caaattgttc aaccattcaa gcggtacttc cgcttaaagc tttacaagaa gcctttggta
     4921 tccagcgagt caattattcg acctatcaag cagtttctgg ttccgggcaa aaaggcattg
     4981 aagatcttca tcgtacgcaa aatgacgagg aaccagtttt gtatccttat aatattagcc
     5041 aaacggtgat tccggaaatc gatcagcctt tggaaaatgg gtatacaaaa gaagaacaga
     5101 aaatgataaa tgagacccgt aagattctgc atcagcctgc tttgccagta tcagcaacct
     5161 gcgtacgtgt gccaatcgaa aatggacatg gagtctctgt ggcagttcaa ctgcaaaaag
     5221 agtttacgac ggaagaaatt agagagtgtc tcagccagtt tccagggatt caattagaag
     5281 atgatctagc agcacataaa tatccgacga gcattcttgc tagaggaaca gatatggttt
     5341 atgttgggag gatcagaaaa gacacttctt tagagaacgg attgttgttc tatacgactg
     5401 ccgataacat acgcaaagga gccgctgcga atgccgtaca gattgctgca gctttaatgc
     5461 agaaaatagc ggaggtggca agatgagtaa tacgctgaaa gtagcgaaat tcggcggaac
     5521 atcgatgtct catgaagagc aattcaaaaa agtccgtaca attgttcttt cagatcccaa
     5581 tcgccaagtg atcgttgttt ctgctttagg caaacgtacg tcagatgatg acaaagtaac
     5641 agacttactt tatttgattt atgcttacct tcagcatcat gttgattgga cacccctttg
     5701 gaaaaggatt tgtgatcgtt tcattcaagt aagagatgtt cttgccctgc agttacctat
     5761 tgaagatgaa ctagcacgca tacagaaaca actggaagac gaaaagataa ctgaagatta
     5821 tttagtaagt agaggtgaat atctgactgc tcaactgatg gctgagtatc ttggttacca
     5881 gtttattgac gcaaaagacc tgattttctt tgactatgat gggaaaatcg atcaggaaaa
     5941 aacagcacag gaaatcacaa aagcatttaa aagatatccg aaaatggttg ttccagggtt
     6001 ttacggggcg aaccctgcag gaaaagaaaa attattgggc cgtgggggaa gcgacattac
     6061 cggcgcgata ctggcgaaga tgttgcaagc ggaaagttat gagaattgga cggatgtttc
     6121 aggaatcatg atggctgatc cgcgaatcat cgatcatcct aaaatcatcg aggaaatctc
     6181 ttttagagaa ctgagagaga tggcttatat gggggctaac gttcttcatg aagcagctgt
     6241 tttcccagtt caagaggctg atatccccat ccaaataaaa aataccaatg atcctttcgc
     6301 tcaagggaca aaaatatcga atcacgaaat agaaaaggaa aatggactga caggaatagc
     6361 tggacgcaag gattttcttt ccatcacttt attcaaacgt catatgtccg atgaaatcgg
     6421 gtttatctgg aaagcaatga gcatttttgc taaacatgag atatcaatcg aacatattcc
     6481 ttcagggatc gacaatatcg gagtgatcgt ctcagcagaa gcgattgccg ataagttatt
     6541 tctcatcacg aaagaattaa aagaaacact tggcgtggaa gaaatcgtcg tgatcgaaga
     6601 cttagctttg atctctgtcg ttgggggtcc gcataaagaa ttgattggat tatctgggaa
     6661 agtgttatcg atattgaaca aattagaaat tcgcacatcg atcttatcac aaggagcaca
     6721 ggagttgaat ctgatcatcg gtgtaccgaa taatcaatat gaaacagtag tgaagggtat
     6781 ctatgaaggg atggtgaata cggatgctga aagaatatcg aatggcactc catcaaattc
     6841 ctgaaatcgg gtttcatgaa ttcaagacaa aaaaatattt atatgaacaa gtaaaaaaca
     6901 gcgaggccgt tatccatgaa atcggcgaaa caggactact cctttatttt gataatcagc
     6961 aagaagaaac gattgctttt cgaacagata tcgatgcact gccagtcaaa gaagaaacga
     7021 atctctcttt tgcttctaag catgaaggtt atatgcatgc atgtggtcat gacggacata
     7081 tggcgatgct tttagggttg actgacttta taactaaaca cattgaggat cttatttata
     7141 atgtagtact gatctatcag ccatcagaag aaatttccgg tggcgcacaa agcgttattc
     7201 agtcaggatt gttggataaa tatgaagtac aagcaatatt tggctttcat ctatggcctg
     7261 gactagctaa gggagaagtt ttctctcgac caggaccttt gatggcacaa agcagtgaaa
     7321 cagacattat cattaaaggc cagtctgcac atattgcttc tagtgaaaaa ggaatcgata
     7381 gtctggaggc agctgtacga tttttgaaga atgtttatga ttttgaccgg caattaccgg
     7441 cagagtggaa tcatctaatg aaatttggcc aaatccaagg tggaacgatc cgaaatgttt
     7501 tagctggaga agttattgta tccggaagta tccgttctta cagttcagat atgcaaaatt
     7561 atttgaagag gggattagac catttagcag aacagtttca ctctacagct actatttctt
     7621 ttcgctataa tgacggctat ccagctgtaa tcaaccatag aggcttatat gcagcactgt
     7681 tagattcaaa caaactacat gaattagatg ctccagttct tcaagccgaa gattttggca
     7741 tatatacgca acaatatcca tgtgtatttt tctttttagg tgtcggtgat acccctgctt
     7801 tacatgatcc gacgttcgat tttgatatgg atgttttaga aaatggtgta gaatggtaca
     7861 aaacgattct ttttacagaa aatcttcttg taaaaatcga tagcagttca aaagaggagc
     7921 gagtaaataa tgatgaattt atccaatcga tttagtccta ggctttctag aatagaagta
     7981 tcaaaaatac gacaattcga tcagcaaata tcgtcgatac cagatgtgat caaactcacc
     8041 ctgggagaac cggattttcc aactcctgaa catgtcaagc aagcaggaat agcagcgatt
     8101 gaagaagact tttctcacta taccggaatg agaggattag aggaactgcg ggaagcggct
     8161 tgtatatttc agcaacagcg ttatggatta acttatgatc cgcaaacgga agttttgacg
     8221 acagttggtg caacggaagc gattgcctct gcgttgttat ctgttttgga agaaggagac
     8281 aaggttctta ttccagcgcc tgcttattca ggatatcagc cattagtgga actagctggt
     8341 gctgaattga tcccgataga tacttctgac acagggtttg tctgtcagcc ggaacaattt
     8401 gaacgagcat ttgaacaata tggatcagct gtcaaagcag tcattttaaa ctatcctaac
     8461 aatcctactg ggacgacatt gtctgcaaaa caattgaaag caatcgctga ggttttaaaa
     8521 aaatatccgg tatttgttat aagtgatgaa gtatatgctg aattgactta tagtggaacg
     8581 catatgtcca tagctactta tcttccagaa caaacaatcg tgataagcgg gttatccaag
     8641 tcacatgcga tgactggttg gcgagtgggc tttatttttg ctcaaaaagc ccttattgac
     8701 gaactaatta aagtccatca atatctggtg actgccgcta caacgatgtc gcaaaaagct
     8761 gcggctgaag cgctaataaa tggggtgaaa gatagcgaga atatgaaaga gcaatatcaa
     8821 acacgaagag actatctggt ccagcaattg gcgcctttag gatttgaagt gacccagcca
     8881 aacggtgcat tttacttgtt ttgcaaactc ccagctgcca ttcaaactga ctcttggcag
     8941 ttttgtgtag aattagcgga acaggcaaaa gttgcgtgta tcccaggaat tgcatttggc
     9001 ccagaaggag aaggttatat ccggatttct tatgctagca gcatggaaaa attacatgag
     9061 gcctgcaaac ggatcaaaga ctttttaaca gaacagtgat tcatagattt tatcaaaaaa
     9121 acaatcgtta attttgcggt atattctgca aggagacgat tgttttttaa atttagttca
     9181 aatgaacgcg tttcttttga ttcataacta atgtaatgtt aagtttaaat tgtttagaat
     9241 gattttaaaa gaatattcaa ggaggtttca tcaatggaaa aaatcaaagt tgtcattgta
     9301 ggtgcatctc atggagggca tcaatcgatc ttggaactat tgacacgata cggtgaaaat
     9361 gtagatatca cgctatttga agcaggagac tatatttcct tcatgtcatg tggtatggag
     9421 ctgtatttag aagacaaggt aacagatgta aatgatgtac gtaatttcag accagaaaac
     9481 tttccgcaat ccaatgttca tattttaaat aatcacgaag taaaaacgat cgatgccgat
     9541 aaaaaaacag taacggttac tagcgcatca gataacgaaa caaaagaata tccttatgac
     9601 aaattgattt tgagttctgg tgtcaaacca aacaatctgc cagtaccagg agcagatcta
     9661 gaaaatgtct acttgatgcg tggatacaat tgggcaacca aaatcaaaga aaaattaaat
     9721 gatccggcta ttaaaaaaat cgctgtcgta ggtgcgggtt acattgggat cgaagcagca
     9781 gaagcaagcc gaaaagctgg gaaagaagtc gtattgcttg atgtgatcga tagaccttta
     9841 ggcacttatt tagatgctga gatgacagat attttagaac aacacctaaa tgaaaacggt
     9901 attgaagttt tgacaaatgc aaaaatcgaa gcttttactg gtaacggaaa agttgaggca
     9961 atcaaaacaa gtgaaaaaga aatcccagca gatctagtca ttcaagctgc cggtgtgaag
    10021 gcaaatacag aatggttaaa agggatcgtc gacttagatg aacgtggctg gatccaaaca
    10081 aatgaatact tgcaaacgaa tcttccagat gtgtatgcag taggagatgc tacacttgct
    10141 tattctatcc cagcacgcaa aaaaatgccg atcgctttag cgactgttgc acgtagagaa
    10201 gcacgttatg tagtaaaaca tctttttgaa gaagtaccaa acaaaccgtt cggcggagtc
    10261 gtaggttcct ctgcattaag tgtctttgac tatcattttg cagcaagcgg attgaatagc
    10321 tttacagcag caaaagcagg agtagatgta cgagtgtctt attatgaaga tacgttacga
    10381 ccaaaatatg taccagaaga acaaggaaat ccaaaagtgt ccatccaatt attctcggat
    10441 ccaatgaccc atcaattgct aggaggagca gtattatcta cctatgatgt tacaggtcaa
    10501 ggaaatgtat tggcactagc gatccaaaac aaactgactt tagaagaact agcagaagct
    10561 gacttcttct tccagccagg ctttgaccga caatggagca tgttgaatct agccgctcaa
    10621 caagcgttag gagaagaacc atttgtagaa taagaaaacg aaatatttga aaagaggttg
    10681 tgaaaaagct gccttgcatc aagtaataag aacagccaag taaaaacagc ttcttatgtt
    10741 tttaacttgg ctggacttat taccgcagat gcaagctttt gaacaccgtt tattttcaaa
    10801 aaagggactg tgacacgatt tgtgtaccga cccccaaaag ttagaccaaa aaatctaact
    10861 tttggaggtc ggtatttttt atggttaaat atacttttga acttaaatta aagatcgttc
    10921 atgattattt ggatggcaaa ggcgattgga ccgtaaaaat ccagatcaag agattgaagc
    10981 ggaaatgctt aaaattcggg agaagcacaa ggattatggc tgtttacgga tgaccaatga
    11041 actaagaaac cgcggtttct ctatcaataa aaagaaagtt cagcgcttga tcaagaagct
    11101 aggaataaaa gtaacggcat acacgcgtaa atcacgtcgc tacaattctt atcgaggaaa
    11161 agt
//
LOCUS       DAYYGO010000093        11105 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000093 DAYYGO010000000
VERSION     DAYYGO010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.095"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..459
                     /locus_tag="ACHA5T_002125"
     CDS             <1..459
                     /locus_tag="ACHA5T_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFM8887681.1"
                     /translation="LLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTTLFVSV
                     IYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGLLPKFM
                     KVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            468..695
                     /locus_tag="ACHA5T_002126"
     CDS             468..695
                     /locus_tag="ACHA5T_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8887682.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            1331..1702
                     /locus_tag="ACHA5T_002127"
     CDS             1331..1702
                     /locus_tag="ACHA5T_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887683.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(1958..2200)
                     /locus_tag="ACHA5T_002128"
     CDS             complement(1958..2200)
                     /locus_tag="ACHA5T_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8887684.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(2232..3134)
                     /locus_tag="ACHA5T_002129"
     CDS             complement(2232..3134)
                     /locus_tag="ACHA5T_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8887685.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(3147..3335)
                     /locus_tag="ACHA5T_002130"
     CDS             complement(3147..3335)
                     /locus_tag="ACHA5T_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFM8887686.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(3349..3912)
                     /gene="amaP"
                     /locus_tag="ACHA5T_002131"
     CDS             complement(3349..3912)
                     /gene="amaP"
                     /locus_tag="ACHA5T_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFM8887687.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(3950..4843)
                     /locus_tag="ACHA5T_002132"
     CDS             complement(3950..4843)
                     /locus_tag="ACHA5T_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8887688.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(4921..5844)
                     /locus_tag="ACHA5T_002133"
     CDS             complement(4921..5844)
                     /locus_tag="ACHA5T_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM8887689.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(5893..6243)
                     /locus_tag="ACHA5T_002134"
     CDS             complement(5893..6243)
                     /locus_tag="ACHA5T_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM8887690.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(6276..7178)
                     /locus_tag="ACHA5T_002135"
     CDS             complement(6276..7178)
                     /locus_tag="ACHA5T_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFM8887691.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(7171..8028)
                     /locus_tag="ACHA5T_002136"
     CDS             complement(7171..8028)
                     /locus_tag="ACHA5T_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFM8887692.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            8362..9171
                     /locus_tag="ACHA5T_002137"
     CDS             8362..9171
                     /locus_tag="ACHA5T_002137"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8887693.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            9211..9708
                     /locus_tag="ACHA5T_002138"
     CDS             9211..9708
                     /locus_tag="ACHA5T_002138"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFM8887694.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(10093..10166)
                     /locus_tag="ACHA5T_002139"
     tRNA            complement(10093..10166)
                     /locus_tag="ACHA5T_002139"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10130..10132),aa:Ile,seq:cat)
     gene            10353..10676
                     /locus_tag="ACHA5T_002140"
     CDS             10353..10676
                     /locus_tag="ACHA5T_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887695.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            10840..11094
                     /locus_tag="ACHA5T_002141"
     CDS             10840..11094
                     /locus_tag="ACHA5T_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887696.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
BASE COUNT         3181 a         2072 c         1898 g         3954 t
ORIGIN      
        1 ttgctattaa aatttgtcgt tttaggtata agtgccgtat ttattttgtt cggcgtactt
       61 ttttatactc agatcgctac aagcgaaaaa ggattcaatt ttgattggca gaccacatta
      121 tttgtttctg tcatttactt tgcccttctc ctagggatcg ttattgctta tttcttattg
      181 aaactattga aaaaaatcga agataaccaa gctttttcca ctcaaagctt aaacatacta
      241 aaaaaaatcg aacgaaccat tttgtttatt ggtttcgcct tttttggctt acttcctaaa
      301 tttatgaaag tagcggattt agacgatgct ccaggtttga tgttggtagg aatcgctgtc
      361 gtattcattc cttttgcagt atacacatta acaagcgtgc tggaaaaact gcttgaacat
      421 gcgattttac ttaagacaga tcatgatttg acagtgtgag gtgcgacatg gcaattatcg
      481 ttaatttaga tgtagtatta gctcaaaaaa aaatgagtgt gactgaattg agtgagaaag
      541 tgggcattac gatggcaaac atctctattc taaaaaatgg aaaagcaaaa gccatccgtt
      601 tttccactct cgaaaaaatc tgccaagttt tggagtgtca accaggtgat ttgttgacct
      661 ataaaaatga ctcgaatact ataccagaag aataaaataa agtagaacaa acagtaaata
      721 aaaaataatc tgaatgatag atgtgtgaga cttttagaag ttatttacgt ctattattca
      781 gattttttat ttcgattgaa taccgtttta tctataaata agatatgtac tgactcatac
      841 aaagggggcc ttttcttctc catgtactat cacttttcct gatacaactg gtttcctgtt
      901 acgttgtagt tggatctgct tttagtataa tactttattc attccaccag actgatagca
      961 gtttgagcag tctaaataat catgtagtat tacaggccat tctgtttcat accttgaagt
     1021 gcttaaaact tcagctaatg gcccattgtg ttaactctta ctccattctt ttttagtaag
     1081 ctcccatata tactgtaaag ccttctatat attatgtcat gttgcttttt aacatttact
     1141 atgtagtttt gataaaatga tttgaataac agtgatggac aattcttttt tatttttaac
     1201 attattttga taataatgat tatttattta aaaatacatt agataacatt tgttacgaca
     1261 cgaaaaagct tcacaaacaa atatcgttat tttaaaatga gggcgaactc acacaattag
     1321 gaggaatagt atgccattta ttgtttactt ttttctttca ttactcgtga tctatattcc
     1381 attaccaaca tttttcctgc tcgtcaatcg tctagcagag caacatgata cgattgctgc
     1441 tcttacgaaa ctttgtttac ttctatttgt ttgtctagat tactttacga gctattattg
     1501 gctaaaacag acaagagtaa aaaagagata ttatttcttc cttgtctttt tcaatctctt
     1561 tgagtttctg atccattgct ttttacttca tcagtatcat acgatccatt tgtggatcat
     1621 ctgggttttc aaagtggtcg tattattttg tatgttcacc tttcctatct caagaaaatt
     1681 tagggaggca ttgttttatt aaagaagctt ctctgtatca ataaagacac atttaaggaa
     1741 aaacttttaa aaatctaatt gggaatttgc ccgcataaat aagaggctgt gacaaaagtc
     1801 ttgtcacagc ctcttattcc gtataaacag tgttcaaaag cttgcatctt cgataatatg
     1861 cccaaccaaa tgaaaaatat gagaagctat ttttgtttgg ctgttcttat tacttgatga
     1921 agaacagctt tttcacgacc tctttttaaa agtcttttta cagttttcga gcaaagaatg
     1981 atacgattag tactaaaatg atggcaccaa tcaatgatgg cacgattgcc attcctgcaa
     2041 tacttggtcc ccaagaacct aataatgctt gaccaagtgc tgatccaact aaacctgcta
     2101 cgacatttgc aataatcccc atcgaagaac ctttattcgt gattgctcct gcaattgctc
     2161 cgataattcc tccgacgata agtgaccaaa taaatcccat gtttcgttcc tcctaatgtt
     2221 ttacttcctt attactgaac ccgtggttct gtattttctt ttactttttg tgcgccgctt
     2281 ccagctgctg attttacttt atctgtttga ttcgaagcaa attcaccggt ttttttcgct
     2341 gcgtcagtta ctttgtcttg caccgtttct tgatcttttt caaattcttc ttttgttttg
     2401 atgtccacta cgttagcgtt gacttcgatc acttctaaat gtgtcatatt ttttacttct
     2461 ttgccgatta cttcttgcat ttcttcaaag attttttcga tatctttgcc atattccaca
     2521 atgacatcca tatcaactgc tacttgtttt ttaccgactt ctgtttcgat cccagccgtt
     2581 acattatctg tattcacaag ttttcctgcc acattagaga agaaaccacc gtttgcagat
     2641 aataaaccgt ctacttgttc aattgcgatt ccgatgattt tttgcacaac tttatcttca
     2701 taattcagct caccgcgcat tgaatcttcc tgttcagaat catgctttcc ttggttattt
     2761 gtatgatcag aaccaagttt tccttgtccg tttgtgtggt cagaactaag tttttcttgt
     2821 gctttcgcat ggtcagaagg atgtttttgc tcttccacag gtttagaagg atcgattgca
     2881 tcatgttgtt ctggaacttt agattctgga gttcctggat gatgaggtgc atgcttttct
     2941 gcatcatgtt tcttctcttc tactccttgt tgaacgttag ggactactgg atctggatgt
     3001 gcagatactg gtgctgtttt gttcttttca tcgttcacac catttgattg gtttggtgtc
     3061 gttgggtctg gatgtgcaga cactggtttt tggttgttca ttttattgtt attatttttg
     3121 ttattgttat tcatggatgt ttcctcctaa gatttaaaat agtgatctaa gattttcgtt
     3181 tgtttcagat aaaaaccgat agcgacgccg atcgctgcca tgatcaagat caataaggtc
     3241 ttgaaaaagc ccacggtgat ccataaaacg gctaatatca atccaagtac cccaaagatg
     3301 atagggagct tgtatgtcga aaatagttct ttcatttttg gtccccccct attctactcg
     3361 tttgcggtca ttgcttcgtt tagctggttc ttcaaagcct gtgtatttca ccgatacttt
     3421 cactggctct tttgttccca atatgtgttg tacttcattt tcgatttctt tcatcaatgt
     3481 tcctgtttta ccaatgagtg ctgaagttcg tttcaactcc ccttttacat tgacggcgat
     3541 cttgtttttc gttgctttga catttacttt tggtgaatgg atgaattcat cttcatgcaa
     3601 gtgagaacga accattcctt caattgcttt tttatccaac gtcaactccc catcaccatg
     3661 tttcagtaga aagtttccga ttgtttttgg ataaaacagg aaaacaagta acgtgataat
     3721 tgtcaaaata gcaaatgcaa cggcaagcca aaatacatat tgttgcacgt atgaattagt
     3781 gatggaaaag aatcgaaact tctctagctg aaaaggcaat ggataggttg cacttaatag
     3841 tatcgcaaac aaaagcacag atagcaaaag taatgttccg atcactccga tcgtttttac
     3901 acctttattc attttcgcac ctcctcatag gggtgaaacg atcaacttct tataagttga
     3961 tcgcttctcc accagttact ccgtaaattt gggctgtaac ataacttgct ttattcgatg
     4021 ctaagaaaac ataaactggt gctaattctc ctggctgacc tgcacgttct agtaaacttt
     4081 gctgaccaaa ttcagggatc gaatcagaag gttgtccttc gtctagttgt aatggtgtcc
     4141 agattggacc aggtgctaca ccatttacac ggattccttt aggtgcaagc tgttttgcta
     4201 aaccgatcgt aaagttagcg attgaagctt ttgttgctgc gtaatctaat aaatgttcgc
     4261 ttggattgaa tgcttgaaca gaagttgttg ttacgattgc tcctcctgct gggatatgcg
     4321 gaacagcttc tttcactaaa gcaaacatag cgatgatgtt tatcataaat gtgtctttga
     4381 cttgctcaag tggtaactca gcaatagact gacaagaaat ttgctgtcct gcgtttagta
     4441 ccatcgtatc tagcccgcca agttcactca cggctttatc tatgatttct ttaggtgctt
     4501 gttcgtcacg aagatcaaaa ggtagtaaga caatcttacg tcctgcaact ttcacgtatt
     4561 ttgctacttc ctctgcatct ttttcttctc ctgggaagaa ttgaagagcg atatctgccc
     4621 cttctctagc aaatgcaatc gctgctgcac gaccgatccc tgaatctcca ccagtgatta
     4681 gtacacggcg gttttctagt tgattattgc cttcatagga ttcttctcca caatcaggaa
     4741 caggaatcat attctgctgt aaagctggtg tatcctgatc ttgtttagga aatttctccg
     4801 tatggtacaa gtttcgcggg tcgattaatt ttggttctgt cattgctgta ttcctcctag
     4861 tttttaaatt gtattgccaa aacaaatttc tttgtttgat acctgtttta aatagattct
     4921 ttaatttgta taatctttcc ggtataaatg gtaacctaaa actactgctg ctatcaaaga
     4981 gccaaagact acgagtataa agcctagagc agaactttta cccggtaatc ctcctgtatt
     5041 cattccccaa atccctgcga acaacgccgg aacggaaatg attaatgcag ctgaatctag
     5101 atatttcatg atatgattta gattattgtc catcatcccg ttgaatagat caccaatact
     5161 ctctaataaa tctcgataga tctcgatcat tttttctgtt tgtcgctgtc tcaaatggac
     5221 atcatagagt agctgttcat cgtctaaacg tttgatgaaa gtttcagaat tccataattg
     5281 atccattgtt tcccgttgat ctcttaaggt atgatcgata tatacgatgt ctctttcaag
     5341 atctgcctgt ttatacagtt cttcattttc cgttgtttta cgagctgctt tatccaaagc
     5401 gtcaattcgc tttttcatct ctcttagttc tttcacgtaa tgggtgtaaa tatctaataa
     5461 tgtatacgtg atgatctttt caaacgagtt taaatgattt ccatgctttg tcagcagccg
     5521 atccacaaat tcggtttttc tatcgataca agtaatcaag agattatcgg aaagtacaaa
     5581 agatattggc gttaattttt tttcgatacg attatctgac tgatctagat taatcactac
     5641 caaagaaaat ggatgctcta gtttcgtctc ataaaggcgc tccatccgag aaacttcttc
     5701 cggatattcg gttcctacga aaatatcatc aggaagctcg aattcttcga ttgcttgctc
     5761 aatctcttcg ggctttgcac tatcaagtac gagccaatta tatttttttt catctgtttc
     5821 tattacattc ttttcttcat ccacaaagta gtatctcaat gtatacctcc tttttggact
     5881 ttctcgaaac tattagctga agaagcagtt ctttaatttg atattataat cgagtactgc
     5941 gatttcattt tctaatgaaa agctgtcaag ctgtttgttg aagtccatgt tcaatcgttt
     6001 accgaaataa ttttgctcta atttagatgt atagatgtcc aataatggat gctcgatttt
     6061 agtcgcatat tctccaaatt ccgaaacaaa aggaatgatt tgaaaaaagc tgccttttgc
     6121 aaaagcacct ttgtccctta gaaatggatc ggaatcattg ataagcgcga gtgcgatttt
     6181 tgtattttcc ccaacctttt caattaacgt ctctactgca tttttagaca ttgtaatttc
     6241 cattcctact ccccctcttc agttgttgtc ttctattatt taacaactac atctgttttt
     6301 ggttgaactt ttgcatattt tggatattta gggaatttag ctacacaacg gaaagccgtc
     6361 atccctgcat atttttcgta atatttcttc gttggccctt caaaatcctt gctccacggt
     6421 ttcacatgtc ctgaaaaatg gataatgtaa ggattgttgc gagtttcttc gtattcacgt
     6481 tcacctgctg ctgttggatg tttttttgct tttgccatga tatagccttg tgcattccaa
     6541 cgcggatgaa gtggtaacca acggtcatgt aagatagcat ttaatgcatc ttggtcatgg
     6601 aatcgtaatt tttctggatt atgttcgatg aaatctaata ctttttgcgt aatattttca
     6661 tctaaccatt ttttcacatt gatcagcatc aaacctgagt tgaaataacg cattgattta
     6721 gctgaaattt tcattttttc cagtcgttga tggaacccag cgtcttcaac agctgctacg
     6781 attgaatcac cgaagtcaag acgccataat ttagagatat cttgtaaagc gatcatgtcg
     6841 caatccatat ataagatacg ttcaacttct gtgccgcgga acaattccgg aatcgcaata
     6901 cgataataag ctgtttctgg aatacgatcg ctgattacca catttgtaaa gaattcttta
     6961 ttgattttca agaattccac atctgagttc attcgtgcgt ttttcactga gaagcgtaaa
     7021 agctgtttac tttcaaaatc gatatcatcg tcaatcacat agaaccgaac aaatttagac
     7081 gggttgcaat tatctaatac tgagacaaat aatgcagcta aatgtggtac gaattttgtg
     7141 ttacaacttg cgactactgc tatttctttt ttattcattg atctcgccta ctttcgttag
     7201 agtactgtgt gctaattttt ttaagtataa attggtatac ggatgatctt tcaacgtatt
     7261 ccaaggttta tcatgtcctg taaagtgaac gatcgaaggt ttttcatttc cttctttata
     7321 ctgacgttca tatttttcat ttggtgctgg atgacgttca aaaatcagcg aagtctgcat
     7381 gttccatttt ggatgaagtt gttcccaatc ttcataaagt acagcattca aagcatcttg
     7441 gtcgtgatag atgattcgat cgccattttc actgaggtaa tggatagttt tttctgtaat
     7501 ttctttttta ttccattgat caatgtcaat caccatcact cctgaattga agtacaagtc
     7561 atcggaatcg attcctaatc tttccaaagc tttcacttgg cctggatcga taatagcgcc
     7621 gattgttttt ccatttaacg gctcatcata tagttttacg atatcgtcta gtactaacac
     7681 atctgaatct aagtaaagga ctttcttata atcctcttta gctaataagt ttggtaaaga
     7741 aattcttaag taagcggtag ttgtgatatg gtcactgact aggaagtctt cgtagatatc
     7801 tttttccact gtcaggaatt ggatacttgc attacttgaa tatttattta ccgtttcttc
     7861 caatccttct ttactatatt ctgatagacc atcgtcaatc acataaaatt ttatgcgtct
     7921 tgttttattg caattttcta atgcagttgc gatcatcacg ctcaagtaag gagcataatt
     7981 ttcgtctgaa gcagttacca caggaactac gccgtatctc ttctccatca aaatcactcc
     8041 tttttctaat ctttttctaa cctgttctac atctagaata ttcctcattt ggttttaaag
     8101 caaaatatat gctttgtttt cattgaatat tttaaaattt ttttatcaca aaacgcatcg
     8161 gtaagcgcta actattcgtt tttcatggct ttttattcaa aggtattgac caaaaactac
     8221 tatatatagc cacctttttc ttaagaatgt tttttgaaaa aattcataat gacaaagcaa
     8281 gttagtttca tttttttaaa ttctcattta atcttaaggt aacaggaaaa tagatatgta
     8341 aaaagaaagt aggaagataa aatgaaacta gcagcgattg atttagacgg taccttgctt
     8401 gatcgaaatg gaaagatacc agaagaaaat atccaagcac ttcggaaatt tgatgaaaat
     8461 ggcggaatcg taaccgtcgc taccggaagg aacagcattt ctgctaaaga tattttttcc
     8521 gaattaggaa tcggtggcta tttgatttct tccaatggtt cactgattgc tgaaatgaag
     8581 gatggaaaaa tggcgaaagt tctcagacgt tcaaaaatag atagcgatac actgagaaaa
     8641 gccttcttct atgctcaaga agcgaaaata tccatcattg ctagccgaga aacccaagat
     8701 gatcagctga cattcgatga atcaaatcta gtcaaagatg acccttatta tgaacatttc
     8761 aatttacaga accattcttt tgatcaaatt ttaaatatgt tggaagatcc ttctcttagc
     8821 tatcttaaat tagcattaac agataagagt gaagaaaaac tacagaagct acaaagagat
     8881 ctgcaagcag atggtatcca atcagtattc tctgatccgc atttcttaga aatcacgcct
     8941 acaggtatca ctaaagctca ttcacttgtt tttctggcgg attaccttgg aatcgaatcg
     9001 agtgacatca tggcttttgg cgatcaagaa aacgacattg ctatgttgaa ttacagcggg
     9061 ttgagtattg cgatggggaa tgcacagcga caagtaaaag aaattgcaaa ccaagtaaca
     9121 ctgacgaatg aagaagctgg tgttgcttat attttaaaaa acttctatta aaaagaataa
     9181 aatgacgata tgaatgtagg aggaaagaaa atgttagtta ttattgatat gcaaaatcat
     9241 attcttgatc caaacagcga attttatatt ccagagtcag atcaattggc agaacgtatt
     9301 gctcaaagat tagctaaagc acgccaaaat aatgaatata ttctcttcac acgggatata
     9361 ccagttgaac tgaaggacaa agaagaagaa cgagaagatt tgcaattgat ccctcttctt
     9421 tctccacaag aaaatgaacg agtgattaaa aagtattact ttacgattcc acctgaaact
     9481 ttagctgaaa tccaggaaac tttatttgaa agcaaagaag aacaaaaaga gatcgaagta
     9541 gtcgggattg aaacaaactt gtgtgtactc tctaatctga taggcctgca gagtgctttt
     9601 cctgaagcag acttttttgt tgatccaatg ttagtctcca gtagaaaaca cggagaatcc
     9661 gctttagagc tgttgaaaga tttcaacgtc tctatcgtcg aatcataaaa atatctatct
     9721 tcatttacga tatttaacac acaataaaaa aagcacgcag aactgtttta gtcctacgtg
     9781 ctttttctat caaagtagat tggctttttt atgacacaga gtgaaacggc aggctcacga
     9841 cctctttctt aaatggtgtt caatcagctg accctcggta ttagcttaac aaacggcaaa
     9901 atatgaggaa ctatttttcc gtttgttaag ctaatactca ggtcaaaacg ctgatttcac
     9961 aacctcttcc ttaaacggta ttcaaaagct tgcatctgca gtaataagaa cagccaagta
    10021 aaaacataag aagctgtttt tacttggctg ttcttattac ttgatgcagg gcagcttttt
    10081 cacaacctct tatgtacctt gtaggactcg aacctacgac cggacggtta tgagccgtct
    10141 gctctaacca actgagctaa aggtacatca taaataaaat ttatttttgt tacttttcaa
    10201 ctatagcacg aatgacaaac tctcacaagt ttcaaaatat aagtaaaata atattgttat
    10261 tcttatttct tttacttgtg tcataactat tttgttactt taatttttat ttgcttattt
    10321 tagaaaaaat gttattcttt tatttattct caatgttagg agtgattatt ttgccattta
    10381 ttgcgatttt attcgattta gttgcggcag cagcttattt tctccaatta aattatcaaa
    10441 cctcaggaat tctgtttatt ggaatgattt ttcaaggtgt gattaccctt ttgcttcttg
    10501 tcatgatgct cagctataaa ggaaaaagat atgcccgtat tcaaacaaaa gtatttgtta
    10561 aatatgtttc cattcgctat gggatcatca ttctttcctt tttagtgaat gcgatcgtcc
    10621 tctttttata tgtattgaat tacttagata tcaatccatt gattttttct cgatagataa
    10681 gacacattca gcggaacagt tcctgcttat cagcagaaaa ctgttctgct ttttcatttt
    10741 tcattgatct attcttcttt tattttccta gcatttcatt tgtgatgtat gcaataacat
    10801 gttatctttt attatatgaa tcgttgagga gtccgttaca tgattacgtt ggtgagagtt
    10861 ttgttttggg tgcctgcggt cgcactggtc gcaagtattg tttatcttat gaattggaac
    10921 aaagaacggt tttatttagc tattttgact cttccggcta tctactttat gtggaaagtt
    10981 tttaattaca actactttga acccgatagt gtttttatag aagaattatc aggactggtt
    11041 ttgtctttat tgatcgtcat tctttatttg atacgtttaa ataaaaaaca ttgagtacta
    11101 ttgat
//
LOCUS       DAYYGO010000094        10701 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000094 DAYYGO010000000
VERSION     DAYYGO010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.096"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(66..290)
                     /locus_tag="ACHA5T_002142"
     CDS             complement(66..290)
                     /locus_tag="ACHA5T_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFM8887697.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(488..2068)
                     /locus_tag="ACHA5T_002143"
     CDS             complement(488..2068)
                     /locus_tag="ACHA5T_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFM8887698.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2469..3845)
                     /locus_tag="ACHA5T_002144"
     CDS             complement(2469..3845)
                     /locus_tag="ACHA5T_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFM8887699.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(3973..5061)
                     /locus_tag="ACHA5T_002145"
     CDS             complement(3973..5061)
                     /locus_tag="ACHA5T_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8887700.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            5233..5667
                     /locus_tag="ACHA5T_002146"
     CDS             5233..5667
                     /locus_tag="ACHA5T_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8887701.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5745..6278)
                     /locus_tag="ACHA5T_002147"
     CDS             complement(5745..6278)
                     /locus_tag="ACHA5T_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFM8887702.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6372..7550)
                     /gene="mutY"
                     /locus_tag="ACHA5T_002148"
     CDS             complement(6372..7550)
                     /gene="mutY"
                     /locus_tag="ACHA5T_002148"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFM8887703.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7543..8340)
                     /gene="recX"
                     /locus_tag="ACHA5T_002149"
     CDS             complement(7543..8340)
                     /gene="recX"
                     /locus_tag="ACHA5T_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFM8887704.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8447..9829
                     /gene="rlmD"
                     /locus_tag="ACHA5T_002150"
     CDS             8447..9829
                     /gene="rlmD"
                     /locus_tag="ACHA5T_002150"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFM8887705.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(10003..10581)
                     /locus_tag="ACHA5T_002151"
     CDS             complement(10003..10581)
                     /locus_tag="ACHA5T_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFM8887706.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
BASE COUNT         3129 a         2179 c         1835 g         3558 t
ORIGIN      
        1 aaatttttgt gaattccagc ttatttcttg gagttaaacg cttctgtctc agcctctttt
       61 tctttctagc tttttgattt ttctcttttc ttttgtactt ttctcttttc attttcagtt
      121 ctacgcgtgt ctttattttt aactttttcc tctttatgga tgattgatcg tactttttgt
      181 gcaatttgct cgtttttgtc ccagtctttt acatctaata atcgacataa tcgtaacatc
      241 tcttcttttt tctgtttttt tctttcacgt ctttgccatt ctgtttccat gtgtcacact
      301 ctcctttctt taatcatatg tacgcaaaat aatttttata tttttttgat tagacatttc
      361 tatgatttta atatagtaaa aagagccgtc acggaaaatc cgtcacggct ctaaacaaaa
      421 tatgagaggc tgtttttgtt tggctattct tattacttga tgcagggcag cttgtgtcaa
      481 gacttcttta catcaaactt tttaatttta cattgggata tttatccgca aaccagcgtt
      541 ctgcaaattg gttctcgaat aagaacaacg gttggtcgaa acggtcacga gccaagatat
      601 tgcgacttga actcatcttc tcatccaaat cttctggatc gatccagcga gcgattttgt
      661 gtcccattgg agacatgatt acttcggcgt tatactcatt taacatacga tattggaata
      721 cttcgaattg tagctgacca acagctccaa tgatatattc ttccgttaga tatgttttat
      781 aaagctgaat cgctccttct tgaactaatt gatagattcc tttgtggaag gatttttgtt
      841 tcatgacatt ttttgcagtc actttcataa acagctctgg agtaaatgaa gggagttctt
      901 cataagcaac ttttagtttt ccttcgaaca atgtatcccc aatctgataa ttgcctgtat
      961 catatacacc gatgatatcg cctgctactg ctttttcgac gttttctctc gaatctgcca
     1021 taaattgtgt gacattgctt aatttcagtt ttttatttgt acgttccaac cagacatcca
     1081 ttcctcgttc gaatgttcca gaacagattc ggacaaaagc gattcggtca cgatgggctg
     1141 gattcatatt tgcttggatc ttgaatacga aacctgaaaa ttcttcctca taaggactta
     1201 ccatctgatc atcttgtgtt ttgtgtccat aaggagctgg tgccaattca acgaaagtct
     1261 ctaaaaaagt ctgtacaccg aagttagtca atgcagaacc aaaaaatact ggtgtctgat
     1321 cgccacgagc aattttttct tgattaaatg catctccagc ctctttgatt agttctactt
     1381 cttctaagac ttgttcatat aaggaatttt cagttagagg atgattttcg ggaagcagtc
     1441 catcttcatc caattctact aatcgttcat cctgataatt ttccggacga taaaactcta
     1501 cacgattatg gtagatatca taaagacctt ctagcccttt acccataccg attggccaat
     1561 tcatcggata agattcgata tctaatagct cttctaattc ttctaacaat tccaatggtt
     1621 cacgaccatc acgatctagt ttgttgataa aagtaaatat cgggatgcca cgttttttta
     1681 caacttggaa taatttcttt gtctgtgctt cgatcccttt tgcactatcg attaccatga
     1741 ccgcactatc cacggccatc aaagtacgat aagtatcttc agaaaaatct tcatgtcctg
     1801 gtgtatccaa gatatttact cgtttccctt gataatcaaa ctgcataaca gaactagtaa
     1861 cagaaatccc acgttgtttc tcgatttcca tccagtctga tttggcaaaa ttacctgttt
     1921 ttttgccttt tacggtcccg gcttgacgga tagccccacc aaacaatagt aattgttctg
     1981 taatcgttgt tttcccagca tccggatggg aaatgatcgc aaaagttctg cgattgtcca
     2041 cttgttgctt taattttgga ttattcataa cctaactcca tttcttccat tcaattattt
     2101 atattttctc taggcacaga ttgtccgatc tcttaaagaa agacaatttc atatagggct
     2161 gtgacaaaag ccgtgtagca gtctcttttt ccgagtaaac ggcgttctag agacagccta
     2221 tcagcaataa gcccacccaa gccaaaaata cgaaaagctg tttttgcttg gctgctctta
     2281 ttacttaatg caggatagct ttttcacaac ctttgtcaac tactacaact gaatcagtat
     2341 agcatggatt cagcgaatga aaaagccacc aaaccataag agtcaaatat ttttttgtca
     2401 gctgactaaa agaaaagacg tgttgcttgt ttattcaagc aacacgtctt tcataaccaa
     2461 gtttccaatt atcttcttgg ctcgtcatct tcgaattctt ttctgaagta tcttcgttct
     2521 tcttcaggtt ccgcttctgt ttcttccgga tcatgaaaaa tgacacggat tttaaggatt
     2581 cgagaacctt ccatttcttc agaaaccagc gtaatattat cgacatcaaa cgataacttt
     2641 tcgccttcat ccggaatcat gcctaatgct gtaatcaaat aaccagccat cgtatctaca
     2701 tcactcatat gcagatcact gtcaaaagct tcattgaact catcaatcag catacgtcct
     2761 tggatgatat attcgtgttc atcgattttt tcataaagat tctcaacttc atctgattca
     2821 tcatcgattt ctccgacgat ttcttctaaa aggtcttcta gtgttgctaa cccaacgacg
     2881 ccgccatatt catctaataa aatagccatc tgattctgcg tctttttcat ttcatataac
     2941 agatcatcaa taaagaccgt ttcaggaacg aataaaggct cctgcatgat tttcttgata
     3001 tctaaattat caaagccgaa tttatgggca gcttttagta aattctttgt atgaaggatt
     3061 ccgactactt tatctttgtc ttcgttataa acagggattc ttgaatagtt ttcggataaa
     3121 acttcattca cattttcttc cacagtgtcg ttgatatcaa tcataaatgc atccgttcgt
     3181 gggaccatga cttctcgggc aactttcgta tcaagagaaa aaaccccttg gagcatttct
     3241 aattcttcat tattcaatac gccttcgttt tcaagcatat aacgcatttc atctctggtc
     3301 atttttgaat cttcatcatc aaaagtcatc ggcgtgatac gcgccaataa acttgtggaa
     3361 gcagaaagca gccagacgaa gggcttagct actacaccaa tcaagcgtat cagccctgaa
     3421 gtgaactttg cgacttcttc tgatttattt aaagcaatac gtttagggta caattctcca
     3481 aaaacaatcg atacataggt caaaatagcc agcacaatga tatttgcaat attttttgcc
     3541 gctgtgccgc cacctaatac cggagctaac cgttccgata aagtattagc tagtgaagca
     3601 ccagacaaaa tgttgaccaa agtgattcct acttggattg tcgataggaa attatttgga
     3661 ttctgcaata tttttaaaag cttttgagat ttcacatctc cttcatcagc tttttgctcg
     3721 attcgattct tattgatcga aacaacagca atttcggaag ctgctaaaaa agcattgatc
     3781 aatgtcaaaa cgatcaataa taaaatctgc gctaacagcg actgactctc agggtcagca
     3841 ttcataatcg cattctctcc ttataaaatt aatttttagt ttacgataga aaaaaacgtg
     3901 gcactatagc cacgttaaaa tggtatcaca aagcaatttt ctttgcaata aaaacaagtg
     3961 tactaaaact acctacgagg tcgtagcaat tttcttcttg ttttcatttt gcttgtcttc
     4021 tttgacgatt ttgattatat aggaaatgat cgtcttgcag attgcgtaaa aaggaacacc
     4081 taagaagata ccaagcaatc cggcaatatt tccagctact agaagaacga taatgatcgt
     4141 taatggatgg attttcagtg atttcccaat tacattagga taaatgatat tcccatccaa
     4201 ctgttggacg attagcacaa ccacacaaca aagtaatgct tttaccggat cattgaagac
     4261 tgttactaga aatgcaggtg ctagtcctag ataaggtcct aaatatggaa ttaggtttgt
     4321 caatcccgca atgaccccaa ataagaaagc ataatccaca ccaattacca tatatcctaa
     4381 aaatgtgaag gtaccaacga ataaacattc gattgcttgt ccgctaatgt aattagccaa
     4441 tgttttattc agttgcccca ataactccac aatttggttt cttctttttt ctgggaaaaa
     4501 ctgttggatg tttggcacta gtttttctcc gtctttcaac atatagaaca agatgaatgg
     4561 tgctgtaatg attactacgg ttgccgaagc gatcgttcca acgatcgaac ctaaactatt
     4621 ggatactcca ctaaggaatt gctgaatgat tgtcccgtaa gaaatgtcta attgctcaaa
     4681 atatttattg atatctactt ccttgaacaa aggcaattcg gctacctctt ttaaccagct
     4741 ttctacctgt ttgatgaaat caggcatatt agatgccaat gaggaaattt gctgtactag
     4801 acttgggatg acactaagta aaatccaaat aattgctgca actaaaagga gcagcacaat
     4861 tgcgacagcc catattcttt tgatttttgc tttcatcaac agcttgacaa taggattcaa
     4921 taaatagtaa agaaagcctg caatcagtac aggtgcaaat aacgtggaaa aaaatgtccc
     4981 gatcggttcg aaaataaagt ttatttctga tgaaatcatg atcaatgcag ctatgatcaa
     5041 taattcaagc gaccaaaaca ttagtttaga ctgtctaagt ttttcaaaca atagatttcc
     5101 tcctttcttt cttactagta tatattatat tcgttaaaaa ttcttttatt ctttttttat
     5161 ttttgaaaac ttgccatact ttttatttgt tttattttcc agtatactat acgaaaggaa
     5221 ggtggctaga ccatgaaagt tgtacaaaca aaagatacaa tgagcaatat ttacttagat
     5281 gctgtacgga tcaggcatca agtttttgta gtcgaacagg gtgtgccttt gtctcgagaa
     5341 atagacaaag atgaagccca ttgtatccac ttcgtcctgt actcagataa aaaagaaccg
     5401 caaggaactg tacgtctttt gcctttagaa aacggaaaaa tgaaattaca gcggatggcg
     5461 attctttctg aatacagaca ccaagggtta ggaaaaatat tgattgaaga agcagaaaac
     5521 tttgctaaga atcaaggcta taatacgatt ttattagggg cacagtccac agctgaaact
     5581 ttttacgaaa aactaggata tacagcttac ggcgatcctt ttgaagatgc tggcatgcct
     5641 catatttata tgaaaaagac tttataaaaa tttatttcga aaaaagcaaa aaacagcaag
     5701 cttttgagaa aaatctctca ctggcttact gttctttttt gtgtttactt acgcgacaat
     5761 tgcttgtaac ggttatacca aatatcaata tagccttccg agaacgggcc atctccatta
     5821 ttgatccagt caaccagaat cttcacattt tcttttagga tgaagtcaat atcgttcggg
     5881 taattcatca ttttgctgtg catctcatat tcatcgacat ccaatagtcg tttctcgccg
     5941 tcaggaaata ccttgatatc caagtcatag tcgatatatt tcaaggcctc atcgtccagc
     6001 aaaaaaggtg aagctagatt acaataatag gaaaccccct tttctctgat cattgctatt
     6061 acattgaacc agtatttttt atgaaaataa acaatcgctg gttcgcgcgt tacccatcgg
     6121 cgtccatcag attcagtgac taatgtgtga tcattcacgc cgatcataga gtgttcgctt
     6181 gtttttaata ccatggtatc tcgccacgtt cgatgcaatt tgccgtcgtg cttgtaactt
     6241 tggatcgtca caaactctcc ttcttttgga actcccatag caacccttcc ttaagcagca
     6301 ttttgtgctt tgtgtttggg tgttcattac accgacatca taattattat atcaataaaa
     6361 atgagctttg cctagaaagg attcctattg tttcgatttt tctctaactg atctaccagt
     6421 ttcatttgga ccttaggaaa aacgaccgaa tcaaatgcat caggcttcaa ccacttcgaa
     6481 gttttgttct cggtaaattc ttgttctgct ccctcggttg cccgtccgta aaaaaggaga
     6541 acatgccatt tcagatgact aaaaatatga gtaacttctc ctaaatgtcg tgtctgccaa
     6601 acaaagagtt cctgcttttc aaatgggaga ttttgctctt cttctgctac taggtcatca
     6661 aacagatcga tttctggctt tgtttcccat tcttttttca atcgctcata ctcctcttgt
     6721 gtcacttcca tcattggaaa tgtccacata ttcgccagca atttttggct atctctttct
     6781 tcaaaataat acgctccaga atgattttga agagctgcac tgatataata aacgtctttt
     6841 ggctttgctt tttttgtttt tacaggaaaa ctcgtttgga ttcctcgttt gttagctaaa
     6901 caaaaggctt ggatcggaca agtctcacac tttggagaag ttggtgtaca gatagctgaa
     6961 cctagatcca tcattgcttg gttgaattca cctggatgct tttcatcaat gattttccgc
     7021 atcgcttcat caaagatttt cctgctagaa gcttttgcga tgtctgcttc aatgcaaaac
     7081 aatctgctga ctactcgcat cacgttgccg tcaactgcag gttctggaag tccaaatgcg
     7141 atacttgcaa tcgcgcctgt cgtatatggt cctattccct tcaatgagct aatttcttca
     7201 ggcgtttgcg gcatttcccc atcaaattct gacatgatct gtttagcagc agcttggatg
     7261 ttccgggctc ttgagtaata accaaggccc tcccatgctt ttaaaagctt ttcttctgga
     7321 gcatttgcta gctcttcaat tgtaggaaac cattccatga aacgataaaa ataatcgatc
     7381 actgtgtcta ctctggtttg ctgaagcatg atctcagaga tccagatacg ataaggatca
     7441 cgattatagc gccaaggcag gtttcttttt tcttgttcat accattgaat gaactgatct
     7501 tgaaattctt ttgtttcttt atctgtccat ttttcccatt ctttattcac tttcctcttc
     7561 cttttccgta atgaatcgct ggatcatatc caaatcaaat cctttttgat ataagctttg
     7621 ctttactttt agatttcttt ttttaggttc aaaccttaaa tttcttcgcc atagtttatc
     7681 tccttgtttg actaaataat catattctat ttcttgatct gcttcttgcg gaagatcagc
     7741 tatcgcttgt tggatcactt cttgagaaaa gcctttcgtc atcaatgttt gttggatttt
     7801 tctcaataat tctttttggc tttttccgtg attttttcca aacgcttttt cggctgtctg
     7861 atgtgcattt tgaacttggt cttcaaatgt atattggctg agcgcttgtt caatgatttc
     7921 aggtttcaaa cctttttgct gcatctgctg cgctaatttc tttggtcctt tgtcacttaa
     7981 gcgcatattc gtgcgcacat aactttcccc atagaccaaa tcatcgacaa ggtccaattc
     8041 ttttaagcgg gctacgattt tatttcgatc ttcttgaagg atttcctttt cttttaagta
     8101 tgttcggatt tctttttccg atcgcaactg ataacttaag taattcattg ctaggtgaaa
     8161 accgaaatca gctccgctac tcttcttgat ctcttggata gtttcttcgt ctaattcccg
     8221 tccttttaat aatcgatagc gtactaatac atcttcagat acttgtaaag cttctccttc
     8281 agataaatct acgcgataga aagggccttt gcctttactg attctcgtaa cggttaacat
     8341 aaacatcctc ctcgaacata tattctctat cataccacag aaaatccccg taatcaggta
     8401 ttatatgtta gaataaacca cagaaaaaag aaaaagggag atacttatgg cagaaacaat
     8461 tgtcaaaact ggacaaacgg ttcctttgaa aatcaagcgt ttaggcataa atggtgaagg
     8521 aatcggttat ttcaaacggt tgatcatatt tgttccatat gcactaccaa aagaagaagt
     8581 actagtgaaa atcacgaaag caactcctcg ctatgcagaa ggagaattaa taaaagtcaa
     8641 aaaaacaagc aaagatcgcg tagtggcacc atgtcctgtt tactatgaat gcggtggctg
     8701 tcaattacaa cacttagctt atcaagctca attggatttt aaaaaggatc tgcttttgca
     8761 agcattggaa aaattcaaac cagcaggctt tcgatcttat caacttttgc cgacgatcgg
     8821 gatggatgag ccttggcatt atagaaacaa agcacaattt cagttacgaa aaaataagca
     8881 gactcaaaaa attgaagctg gtttatacga agcaaactct catcagttag tccctataac
     8941 agactgcttg gttcaagaac ctcgaacaca aaaagtaatg aatactgttg ttcaactatt
     9001 gaataaattt gatgcaccca tctatgatga acggacaaac agtggaatct tccgcacaat
     9061 catggttaga atcggtgtta aaactgggga gttacaagtt gtttttatta ctcgcagtaa
     9121 aaaattcccg caaaaaaacg caatgatccg tgagatcaat gaacgactgc ccgaagttgt
     9181 ttcaatcatg caaaatgtcc aggcacaaaa aacgtctctc gtgatggggg atgaaacgat
     9241 ccacttatgg ggaaaagaag ccattgaaga acaaatcaat gaagtgactt tcgatctttc
     9301 tcctcgtgcc ttctttcaac tcaatcctca gcagaccgaa ttgctttatg gcgaaggaat
     9361 caaagcatta gatgtccagc ctaatgaaac gatcgtggat gcttattgcg gagttgggac
     9421 gatcggctta agtgtagcaa aacaagccaa agaagtacgg ggaatggata ccgttcctca
     9481 agcaatcgct gatgcaaaaa aaaatgcgga aagattagga tatcacaaca ctcgatatga
     9541 agtcgggaca gcagagcaag ttctgcctaa atggttgaat gaaggttttc agcctgatgg
     9601 tatcatcgta gatccaccac gtacgggttt ggataacgca ttgatccaat cattattgaa
     9661 ataccctcca aaaaaacttg tttatatctc ctgtaatgtt tctacacttg ccagagatct
     9721 ggttaattta agtaaaaagt tcaagattga atatttacag tccgtcgaca tgtttcctca
     9781 gactgcacgt tgtgaagttg tcgtgaagat gacgagactc gctgactaaa tgaaagaggt
     9841 tgtgaaaaag tctgccctgt atcaagtaat aagaacagcc aaacaaaaat agctcttcat
     9901 gttttatgaa tactgtactt gtgaccgaag agttaacagg aggacactat ttatttaaaa
     9961 aaagggttgt gacatagttt ttgtcacagc cctttttgat tattattgga tattcacgtc
    10021 tatcacttga taaaatgcat ttgtcgtatc gctgatattc catactgcta aaatcacatt
    10081 atatcctttt cgatcctgcg gaagtgtgat cgtttggttg actgtttttg gtggaacagt
    10141 tgctttgtcg tctatttttg tgatcaattc aaaatcacta aatttcaaag gttgattagg
    10201 attccaagtt ggtttggtaa tgtagtaatc ccaagtactt gtccgatgtt gtgctgttaa
    10261 attccaagta atatttaatg ttcctggatt aaccatgttt ttatgccatc tgttagatgt
    10321 ctgttcatct aaaggctcaa agccgctgac tcctgcacta gcaatttttc catcaatgaa
    10381 tgtatttttg gtagcttcga tactttgcgg ttcccattgt gctcttccga cattttgatt
    10441 taagtttcct gctgctgatg ttcctaggta tgcacggctt cctggagaag taatataacc
    10501 atgcgcaaaa cctggaatgc taaaaccgaa caaccctaaa ctaactaacc caataaacaa
    10561 taacgtacta atctttttca tgtagaaacc tccttcattt gataaccaca ttgtaaacgc
    10621 tttattgtat taacacaaac attatgcttt tataatttta tgttgaagta aaaaaggtga
    10681 aaaaagtaag atgcagatta t
//
LOCUS       DAYYGO010000095        10369 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000095 DAYYGO010000000
VERSION     DAYYGO010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.097"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACHA5T_002152"
     CDS             6..305
                     /locus_tag="ACHA5T_002152"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8887707.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACHA5T_002153"
     CDS             378..1739
                     /locus_tag="ACHA5T_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFM8887708.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACHA5T_002154"
     CDS             1761..2021
                     /locus_tag="ACHA5T_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887709.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACHA5T_002155"
     CDS             2035..2787
                     /locus_tag="ACHA5T_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFM8887710.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACHA5T_002156"
     CDS             complement(2881..3486)
                     /locus_tag="ACHA5T_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8887711.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACHA5T_002157"
     CDS             complement(3539..4513)
                     /locus_tag="ACHA5T_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFM8887712.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACHA5T_002158"
     CDS             complement(4891..5610)
                     /locus_tag="ACHA5T_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8887713.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACHA5T_002159"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACHA5T_002159"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFM8887714.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACHA5T_002160"
     CDS             7161..8558
                     /locus_tag="ACHA5T_002160"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8887715.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACHA5T_002161"
     CDS             8639..9079
                     /locus_tag="ACHA5T_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8887716.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ACHA5T_002162"
     CDS             9227..9553
                     /locus_tag="ACHA5T_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8887717.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ACHA5T_002163"
     CDS             9554..10303
                     /locus_tag="ACHA5T_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFM8887718.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
BASE COUNT         3288 a         1829 c         2149 g         3103 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgctttt
//
LOCUS       DAYYGO010000096        10318 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000096 DAYYGO010000000
VERSION     DAYYGO010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.098"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(71..904)
                     /locus_tag="ACHA5T_002164"
     CDS             complement(71..904)
                     /locus_tag="ACHA5T_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFM8887719.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(1208..3373)
                     /locus_tag="ACHA5T_002165"
     CDS             complement(1208..3373)
                     /locus_tag="ACHA5T_002165"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFM8887720.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            3633..5927
                     /locus_tag="ACHA5T_002166"
     CDS             3633..5927
                     /locus_tag="ACHA5T_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFM8887721.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            5920..6585
                     /gene="pgmB"
                     /locus_tag="ACHA5T_002167"
     CDS             5920..6585
                     /gene="pgmB"
                     /locus_tag="ACHA5T_002167"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFM8887722.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            6578..7585
                     /locus_tag="ACHA5T_002168"
     CDS             6578..7585
                     /locus_tag="ACHA5T_002168"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM8887723.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            7607..8626
                     /locus_tag="ACHA5T_002169"
     CDS             7607..8626
                     /locus_tag="ACHA5T_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8887724.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(8907..10274)
                     /gene="nhaC"
                     /locus_tag="ACHA5T_002170"
     CDS             complement(8907..10274)
                     /gene="nhaC"
                     /locus_tag="ACHA5T_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFM8887725.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTVALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
BASE COUNT         3199 a         2242 c         1908 g         2969 t
ORIGIN      
        1 ctctccaaac ggtataatcg tttggagagt tttattttaa tcagtttttt tatgttatat
       61 gttatatttt ttattcgttc acatctaatt cgatttccac cccgtagtga tcactgatga
      121 ttggactatc gatcccatca aaaatccgtt gatatttttt aacctgcatt ccttcaggga
      181 cgaaaataaa atctatacgc aatttttctg tattttgttc ccaaccatct attttctttt
      241 ccacagtagc tgcttttcct ttttcattcg cgacaacata cgaatcttga agaagaaggc
      301 aactttctct tacagtataa taaccttgag agtctactgg attattgaaa tcacctagga
      361 aaaaaagtgg tacgcgagtt tccaaaaagt agttttccaa gtttttccac tcaaaataaa
      421 agccactttt tttatctgtc caccaagaaa agtggcagct cgagacaaaa atgtcctgat
      481 catctatctt tgttttccca atcaagatct tgcgtgtctg ataatcttct ttcttttgaa
      541 attgggagac ggtaaggacc tgactttcca atgttttttt cgtaagcagt gcatttcctt
      601 cttcatattt atcataaccg atgtggctca tttcccaact ccaaaaatac tcttttccgt
      661 gttcctttaa aagctgaact aaacggtagg caaaattatc tttacggata ggtacttgat
      721 tctttaccgg acagaacttc attagcttcg caggttctac ttcttcagat tccagtaatt
      781 ggttgatttc ttgtaaagca atcacttcat aatcttctaa caggatctta tctgctaatt
      841 cttgcaattt tttctctggt tctggctcaa gccagctatg cgtgttgagt gtcatgaatt
      901 tcattggttt tctccttgtc ttcatttcga aacaaaaggg ttgtgacaaa atttttgtca
      961 caacctctcc agcaaatagt gttctcctgc taactcctca gtaataagct caatggacca
     1021 ggagctattt tttgtttggc tgtttttatc acttggtgca gggcagcttt ttcacgacct
     1081 ctccactaaa cagtattcaa actgctgctt ctcggtaata agcccaacca aacgaaaaat
     1141 aggaggagct atttttgttt ggctgttctt atgacttgat gcaggacagc tttttcacaa
     1201 cctctcctta cattttaatg gagccgatca ctttaccagc tgtcactgta cctaattgct
     1261 tgatcactac ttcttctact ttatcgtttg taaatacgat catgatcgtt ggatcttttt
     1321 cttcgttttg gatcacatca agatccatct cagctaattt cgttcctgtt cctacttctt
     1381 ggccttcttt gacagcaatc tcaaaagctg gctgattcat ttggactgta tcgattccca
     1441 tatggatcaa aacttcgata ccatctttcg tttccagccc aattgcatgt ttgctaggga
     1501 aaatacttgt cacttttcca gcgatcggcg caacaacttc tcttgatgtt ggaataacag
     1561 caaagccgtc acccatcatt ttttgtgaga ataccggatc ttttacttca gataaaggaa
     1621 tcacttctcc agaagctgcg gaaactagtt cttgttctat acctaagtat tgcttactac
     1681 ctgcttgtcc atttcctgta gaaaccattt ctggctctgg aatctctgct cctgattgca
     1741 gcagatcttc gatatctgat ttcaagacat ctgcttttgg tccataaact gcttggactc
     1801 cattgtcttt gacgatcaat cccatcgccc ctgctcgttt ccaggcttct tcagtcccta
     1861 ctttttctcg atcatttaca cttacgcgta accgagtcat acaagcatcg acatctttga
     1921 tattttcttt tcctcccagc aaatgaatga tctggaccac ttgctgatct gctgttcctg
     1981 ttccgctgtt agatacaacg gtttcttcac tgtcattgtc gtaatttccg ttacgtcccg
     2041 gtgtagcaaa gttgaatttt ttgatcaaga aatttgctaa gaaatacgta acaacaccaa
     2101 aagcgatcac acacaacacg aagttgataa gatctccacc tagacccgct ttgatagcta
     2161 acggtgtccg agtcagcaat tcgatattcc caaatgaatg gacccgcaat ggcaagatat
     2221 ctgccatcgc aaatgcagcc ccttggatca ctgaataaat gacatataat ggcacagcag
     2281 caaacataaa catgaactcc aatggttctg ttacacctgt tagaaagaca gccaacgctg
     2341 ctgaaaaata cattgattta tatcttgctt tcttatcagg atcgacatta cgatacatcg
     2401 caaaagccat tcccatcaag atacctgaag aaccaatcat ttgtcctact ttgaaacgag
     2461 ccggcgtcca gttatgcaac acaaactcat attgcgacat atcccctgca cctttaagat
     2521 tcactaaatc agtggcccaa gccaaccata acggatcttg cccaaatact tgtgtccccg
     2581 cctgtgcgcc tgataaaatc tcatacgatc cgccaagctg tgtatagttg ataggaatcg
     2641 tcagcatatg atgcaatcca aatggcaata gtaaacgttc taatgttcca aataaaaacg
     2701 gtgctagaat cggtgcgctt tcttgtgatt ctgcaatcca taaaccaaaa ttattgattc
     2761 cagcctgtac gtttggccac accacagcaa gaatcaatgc cacgattgtt gaccataaga
     2821 taacaacaaa cggaacaaag cgctttccat tgaaaaatga caaagcatcc ggcaattttc
     2881 tataattata gtacttatta taagccattg ccccgacaaa tccagcgatg atccctacga
     2941 atactcccat attcaaagct ggtgcttcca atacacttgt aaaaaatcct ttaaccatga
     3001 tcttcgtacc gaacagagtg tgggtaaatg catcttcatt cgcaagcatg tcacttgtga
     3061 caccaaagat agccccagtg atccgattga ttaaaataaa tgagatccca gctgcaaatg
     3121 ctccgcctgc tctttctttt gcccaacttc ctcctattgc taaagcaaat aaaatatgaa
     3181 gattaccaat aattgcccaa ccaatatttt caattactcc gcccgttgtg acaagcggcg
     3241 tccaatcagg attgatcaag ggaattgatt tccctatact gatcatcaac ccagctgccg
     3301 gcataactgc taccacaacc attaaagcct ttccaaattt ttgccaaaac tcaaaactaa
     3361 atatcttttt cattccgttt agctccttta cgcaatcggt ttcgttaaac gtattatagc
     3421 gtgcgttttc ttttttgtaa atgctttatt ttttattttt ctgtctatac cattctataa
     3481 aacctataat acgtgtgttc ccttctataa ttttctccta aaaatttatt tttttaagct
     3541 tttttctatg aaatattttc ttttgtctat tgacttgtgc aatcggttgc gtttattatg
     3601 aacgtataaa acagctcatt aggaggagaa aaatgaaaca gatcaaacga ctatttgcta
     3661 ttgatccatg gaaaatcagc acgacacaat tagataaaaa aaatcttcga ctgcaagaat
     3721 ctttaacaag tattggaaac ggttatatgg ggatgcgcgg gaactttgaa gaaacttact
     3781 caggggacca ccatcaaggt acttatttgg cgggtgtctg gtatcccgat aagacacgtg
     3841 tcggctggtg gaaaaatggt tatccagaat actttggaaa agtgatcaac gccttgaact
     3901 ttatcgcgat agatatttcg atcaacgatc agctgatcga cttggcttct ttggaaccag
     3961 aagactttta ctgggaatta gatatgaaaa atggtgtatt atctcgtaca ttcacaatca
     4021 ctacttctac taataaagta cgcatttcct tcgaacgttt tctgagcatt gtcaaaaaag
     4081 aatcagctta tatctattta actatagaaa tgcttgaagg tagcggaaca gtcaaactcg
     4141 tatcaaaatt ggatggaaat gttcaaaacg aagacagcaa ctatgatgaa catttttgga
     4201 atgagattga tcgtggaact atagaagaaa tcggctattt gacgactaaa actattccaa
     4261 acaactttgg gattgaacaa tttactgtta ctgcagcaat gcgccatttc gttaatggaa
     4321 aatcagtcat ccctgactat cacgaagata ctttagcatt acatggttgc ttgtctgcag
     4381 aattatctca aggtgagaca tggtcagtgg aaaaagaaat tatcgttctt actagtagag
     4441 atattccaga agcgcagcaa gcagaaaaag ctgtctcaga attaacgaac cagacaaaca
     4501 attatatcca agcaaaagaa gagcaaacag cggcttgggc agaacgatgg aagcttgccg
     4561 atgtggtcat cgaaggagac gaagaagcac aacaaggtat tcgattcaat ttgttccaac
     4621 tattctctac ttactacggc gaagatgaac gattgaatat cggccccaaa ggattcacag
     4681 gagaaaaata cggcggagcc acttactggg atacagaagc gtatgctgtc cctctttatc
     4741 ttgctttagc caaacctgaa gtaactaaga atctgctgaa atatcggcat aatcaactgc
     4801 cgcaagcaat ccacaacgct cagcaacaag gattaaaagg cgcgctttat cctatggtca
     4861 ccttcactgg tgtcgaatgc cataacgagt gggaaatcac attcgaggag attcaccgaa
     4921 atggcgcgat tgcctatgca atctacaatt atacgaatta caccggcgat acggaatatt
     4981 tgaaaaatga aggactggaa gttttagcag aaatcgcacg cttctgggca gaccgtgtcc
     5041 atttttcaaa acgagcaaat aaatacatga tccacggtgt gaccggccca aacgaatatg
     5101 agaacaatat caataacaat tggtatacaa atacgatcgc agcttgggtc ttgcgttaca
     5161 cgcgagaaaa ttatttaact tatcaaaaag aagctgctat cactatcagc gaagaagaac
     5221 tagccaaatg gaaagatatc gcagagaaca tgtacttccc agttgacgaa gaattaggaa
     5281 tctttgtcca gcatgacaca ttcttagata aagatctcat gcctgtatcc gatattccca
     5341 caagcgatct tcctctgaat cagcattggt cttgggacaa aatccttaga tcttgcttca
     5401 tcaagcaggc agatgtacta caggggattt atttcttcaa tgatactttt acaaaagaag
     5461 aaaagcaaaa aaactttgat ttctatgaac caatgactgt ccatgagtct tccctttcgc
     5521 caagtatcca ttcgatttta gcagcagaat taggaatgga aagcaaagct gtagaaatgt
     5581 accagcgaac agctcgcttg gatttggata attacaataa cgacacagaa gacggcttgc
     5641 acatcacttc catgactggt agctggttgt cgattgtcca aggatttgct caaatgaaaa
     5701 ctgcccatga tcagttgaga ttcgcaccat ttctgccaaa aacttggaaa gcttattctt
     5761 tccatatcaa ttatcgcgga cgtttattgt tcatctcagt cacagatgaa caagttactt
     5821 tcgagttatt agaaggttct cctctagaaa tttacgtcta tgaacagtct tattctttag
     5881 cggatcgtct agaaataacg ctaagaaagg aggaagcaaa tgtttaaagg ggtattgttt
     5941 gatttagacg gtgtcattac cgatactgct gaattccatt atcgtgcttg gcgaaagctt
     6001 ggtcaggaaa tcggcattac gatcgaccac acattcaacg aacagctaaa aggcgtcagc
     6061 cgagaagact cactctcttt gcttttagct tatggcggaa aagaacactc tttttcaaag
     6121 gaagaatttg cagaattagc gaaaagaaaa aacgattatt atttagagat gatccagacg
     6181 atcgaaccaa aagatgtctt ccccggggtc gtgcctttac tggattcttt aaaagaagaa
     6241 aaaatcaaga ttgctcttgc ttctgcaagt aaaaacgggc cattcctact tgaaaaaatg
     6301 ggtcttacac cttactttga tgctattgcc gacccggcag aagcaacaaa cggaaaacct
     6361 gcgccagata tcttcctcct tgcagcaaaa gctgtcggac taaaagcaga agaatgtatc
     6421 gggatcgaag acgcgcaagc cggtatccaa gccatcctct caagtggcgc tcaaccaatt
     6481 ggcgtgggac gtgctgaaga ccttggagag gagatcccgc ttgtccctga tacgactttt
     6541 cttacgatcg attacttgaa agaaaagtgg catgaccatg gataatccag agatctccgt
     6601 cgtaccagtt ccagagactg aaataaagaa aatcacattg aagaacgact acttagaagt
     6661 cgttcttctc aattacggtg cccgcctgca ccaaatcttc gcaccagaca aagaaggaaa
     6721 atcagaaaat gttttattgt cttacgatcg tttcgaagat gttctaacag acaaaagctt
     6781 ttttggagca actgtcggac ctgtagccgg aaggatccgg gatgcttctt ggggtgagca
     6841 tagattggag aaaaactgcg gatcacacca tatccacggt ggcacgaaag gctggtcttt
     6901 ccaattttgg gaagtagaag ttttcaaaac tccatcatca ataggtgtgg tcttttactt
     6961 aaaagatgaa ttttctacct atccaggacc aatcactgct acgatcactt accgtttaac
     7021 aaaaaacgaa ttagagatga caacctctgc tagtagcctt gttgaaacca tctgcaatcc
     7081 gaccaaccat gcctacttca acctttcagg aaatggaaca agagacatct atgaacatca
     7141 gttaacggtc tttttagatg gtgtgcttga attagatcaa gaaaaactgc ctactggaaa
     7201 ttggacaaaa aaagaaaaac tgccaatcga ctttagaaaa tccccaacac tgcaagagat
     7261 ccttgcatgt tatccagatg gactagatga tgtatttctt ttgcatcatc ctaggctttc
     7321 caaaacttcc ttacaattat tcgagaagca ttctggtcgc caaatgacta tagcaacttc
     7381 taataaaagt atggtattat tttctacgac tggattcgaa gctgatttct ctgtcaatgg
     7441 aaaacagatg cattcaaact acggattagc aatcgaaccg caagaaatcc ctgatatcgt
     7501 tcatttcccc aaatttggat cgatcaacct ccatcctggt caagaacgga tcagtcagac
     7561 gatttatcgg ttctctgcac aataacaaga gaaaggtgtt tttatcatgg ctattaccgt
     7621 aaaagatgtg gcgaaaaaag ccggtgttgc cacctcgacc gtctctcgtg tcatcaacga
     7681 tcatcctagt atttccgaga caacaaagaa gaaagtccga aaaattatgg atgaactagg
     7741 ttatgttcct aacatgactg cccgcaatct aggaaaacgc atttccagtg ccatcggcgt
     7801 aatcttaccc cctcttgatt caaaagagcg gatcggtaac ccattttatc ttgagatcat
     7861 ggaagcaatc aacgaagaag cgcggaatta tgatatgact acagcaatcg ctactgctaa
     7921 aaattttgaa ttgctgttag agaacgtcca gcgtatgcat ttacaaaaac aagtagatgg
     7981 ttttattcta gtgtattcag atagtcaaga tactgtcatt gattatttat accaaaacca
     8041 catccctttc actttgatcg gacagcctta tcatcatgag aacgaaatcg tttatgtaga
     8101 taatgataac cagcttctag gtaaacaagc aacagaattt ttgatccaga atggacataa
     8161 gaatatcctg tttgtgacaa acacgactca tgaaagtttg tatttcgaac gatatttcgg
     8221 ttatcaaaaa gcaatgatga tggctggtct tcctgcgtta tccagtgtag tactagagca
     8281 tccggaggat tatctgtcct ttgagaaaac gttgaaagaa aacgaagcca cagcattggt
     8341 ggtcattgac gatatcttcg cccttcgcac gatgcagttg gcgcaaatgt atggctatgt
     8401 cattccagag acactctccg tcattagctt caacaactcg atcttctcaa cattaacaca
     8461 cccttacctt acaagtatcg atatcgatat ctccgaatta ggacgtgtcg ctatgcaaaa
     8521 gctgcgcgaa ttgatccatg aagaattgag cagtggcgta cgtttggtca tccctcatcg
     8581 cttgatcaaa agagagacag tcgttccttt agaaaaccca tactaaatag ctgactcact
     8641 aaatacgctg accatttatc ctatggtcag ctttttatgt aaaagaagat actacttcta
     8701 acacattgtt tatctgtaaa aaagagcgtg ggacaaaagt aaagaacact tttgtctcac
     8761 gctctaaaca cgtataaacg gcggaagcag aagcaactcc ttcggaaata agctgaaatt
     8821 cacaaaaatt taaaaagcaa ttttcttgag tttcttctta tttcttggag ttaaacgctt
     8881 ctgtcccagc ctcttttttc cttccttcaa ttttttacac gtcgacgatt caataaaaaa
     8941 ccgcctaata ttgtaaagac aggtgcaaga atcgagaaaa aggcgaatgg caagtaatgc
     9001 aacgcgctaa ctttcatcgt tcccataatg aatgtgccgc tgactcccca cggtacgaga
     9061 gaattgacgg tcgcacctga atccgctagt gttcgtgtca aataggtttt tgataagcca
     9121 acgtcatcaa aagaacgctt gaatgtttct cctggaagaa tgatggacag atactgttct
     9181 ccaacgatca gattgattcc gatacagctc aaggcagtaa acccgactaa acgagaaggt
     9241 cggtccatct tttcttttaa ttcttgaacg atggtatcaa cgatcagata tttgatcagt
     9301 aacccaccta atgccaatgc aagtaaaatc aaactagcag aacctaacat actcatgatc
     9361 ccgcctcgag aaagcagttc atctagcttt ggatcaggtg tatttgcgac ataaccattc
     9421 atcaagatgt ctgctgcttt tccgaccgtc aaacttttct catggataaa agaaagaacg
     9481 agagcaactg tcgatcccgc tagtaatgta gggatagccg gaattttttt gattgccccc
     9541 ccaaataaaa taactagtgg aatcaatgtc caaggagaga tccaaaagct cgctttcatg
     9601 ttttggatca tttcattgat actagctaga tcggctgaat gactgttatg tcctaatatc
     9661 gtataaaaaa tcaacgaaag gacgaatgct gggaaatccg tataacgcat accagcgata
     9721 tgttcgaata gattaactcc tccaataccc gctgccaagt tggtcgtatc tgataaaggc
     9781 gagcagttat tccctaaaaa ggcaccggaa acgactgcgc ctgtagtcat tgcatcattg
     9841 aatccaagta aatgaccgat ccccaaaaaa gcaattccaa tcgtcgaaat cgttgtaaat
     9901 gaactgccga cagtcactcc gacaattgca caaacaagaa atgctgtcgg taaaaagaaa
     9961 cgcacagaaa caagtttaaa tccataaacc ataatcgtag ggatggttcc agataaaatc
    10021 catgaagcaa ctaagacacc aatcaaaaga aaaatcagga ttggaatgat ccctggagtg
    10081 attcctttga tgatcccgtc atggatctca tcccatgtga actttttaaa tctgccataa
    10141 aacaacaaac acatgaaagc tgcaaggatc gggatatgtg gcgagagctg gaatccgatg
    10201 atcagtactc ctaggagtgc caacaacacg atcaatacag cgatactttc ttttaaagaa
    10261 atatttttat tcatcgtcga tctcctattt caaatgaaaa ggccctgcca tgaaaaaa
//
LOCUS       DAYYGO010000097        10118 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000097 DAYYGO010000000
VERSION     DAYYGO010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10118
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.099"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            253..522
                     /gene="rpsN"
                     /locus_tag="ACHA5T_002171"
     CDS             253..522
                     /gene="rpsN"
                     /locus_tag="ACHA5T_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFM8887726.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            537..716
                     /gene="rpmF"
                     /locus_tag="ACHA5T_002172"
     CDS             537..716
                     /gene="rpmF"
                     /locus_tag="ACHA5T_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFM8887727.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            748..1125
                     /locus_tag="ACHA5T_002173"
     CDS             748..1125
                     /locus_tag="ACHA5T_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887728.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1146..1295
                     /gene="rpmG"
                     /locus_tag="ACHA5T_002174"
     CDS             1146..1295
                     /gene="rpmG"
                     /locus_tag="ACHA5T_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM8887729.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1319..2302
                     /locus_tag="ACHA5T_002175"
     CDS             1319..2302
                     /locus_tag="ACHA5T_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFM8887730.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVIADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLEKFNHPLVFESDSSENH
                     YNGKHYHQHHQEHEFKSILLTSDQDIKKDFLFQLIDRLILTNQGSLYRFKGLIALQDQ
                     DFIVAIQGVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2332..2454
                     /locus_tag="ACHA5T_002176"
     CDS             2332..2454
                     /locus_tag="ACHA5T_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFM8887731.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKQNKMKKLA"
     gene            2472..3995
                     /locus_tag="ACHA5T_002177"
     CDS             2472..3995
                     /locus_tag="ACHA5T_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFM8887732.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLIQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKLNKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNEMHNWPTFYPASLSEHEIAQEMMAH"
     gene            4019..4258
                     /locus_tag="ACHA5T_002178"
     CDS             4019..4258
                     /locus_tag="ACHA5T_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8887733.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYQFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4451..5014)
                     /locus_tag="ACHA5T_002179"
     CDS             complement(4451..5014)
                     /locus_tag="ACHA5T_002179"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8887734.1"
                     /translation="MDFSISFITIYRAICNDLFDTEPLSHGNKGLIRSLHHRGKTRHT
                     KNHVESRGKICLTHSIHGHPKSADDRSDFGHWKADIVAGKTSGTCLVTLTDRKSCFLL
                     AGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITPDRGKEFSNHFAVTEVLNNMPFYFP
                     DPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5107..5325)
                     /locus_tag="ACHA5T_002180"
     CDS             complement(5107..5325)
                     /locus_tag="ACHA5T_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8887735.1"
                     /translation="MSQYCHLTILKRERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTNHLPKRKKIMLIEEKNADVITF"
     gene            5514..6461
                     /locus_tag="ACHA5T_002181"
     CDS             5514..6461
                     /locus_tag="ACHA5T_002181"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFM8887736.1"
                     /translation="MYGDHGKEIAVIVNDIGEVNVDGVLVDKKGFKRTGEKLVSMPNG
                     CICCTLREDLIVELDQLVQFSDLNQIVIEASGISKPIPIIQAIILAKNPLDIDLTQRL
                     EIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVVEEEQDIRGLLIDQFECCNILLLNN
                     SDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVDLSKILNTNLFNIDETSYSMGWFKE
                     LELGYQNHIPETEEYGISSFIYRNRLLLDAQKFAHLTDKNFPGNITRAKGFLWFAQHS
                     DFSTIFETSGSHKSIESLNYWVASLPKIECQMIIQKNPN"
     gene            6428..6517
                     /locus_tag="ACHA5T_002182"
                     /pseudo
     CDS             6428..6517
                     /locus_tag="ACHA5T_002182"
                     /inference="COORDINATES: protein motif:HMM:NF019303.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6650..7057
                     /locus_tag="ACHA5T_002183"
     CDS             6650..7057
                     /locus_tag="ACHA5T_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFM8887737.1"
                     /translation="MKFLRKAGVKIGDVIIIGILIIPSFIPLFVFSIEKENETTEDAI
                     KYAIVQIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVR
                     TGWISKPGQTSICLPHKLVISIEKKESKDYYIY"
     gene            complement(7298..8638)
                     /locus_tag="ACHA5T_002184"
     CDS             complement(7298..8638)
                     /locus_tag="ACHA5T_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM8887738.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            complement(8914..9069)
                     /locus_tag="ACHA5T_002185"
     CDS             complement(8914..9069)
                     /locus_tag="ACHA5T_002185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFM8887739.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLTDHHITHSMSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(9076..9222)
                     /locus_tag="ACHA5T_002186"
     CDS             complement(9076..9222)
                     /locus_tag="ACHA5T_002186"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887740.1"
                     /translation="MTDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
     gene            complement(9253..9456)
                     /locus_tag="ACHA5T_002187"
     CDS             complement(9253..9456)
                     /locus_tag="ACHA5T_002187"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8887741.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKMLNR"
     gene            complement(9366..9698)
                     /locus_tag="ACHA5T_002188"
     CDS             complement(9366..9698)
                     /locus_tag="ACHA5T_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8887742.1"
                     /translation="MKKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
BASE COUNT         3360 a         1613 c         1996 g         3149 t
ORIGIN      
        1 gtatttgtgt ttgattggac taataaaagt gaattcttat ttttcagagc ttaaactgca
       61 tattctttta aatgatttga ttatctctat gtaccgataa attcgttttc aaatagttat
      121 ctattcatat gagttttatt tcatgttatt tattgttgac aagagcaact cttgttggta
      181 aagtatcagt gtcaaaacgt aacgattacg atttgagatg tctaaatcaa aataaaagga
      241 gtataaaggt atatggcaaa aaagtcgaaa attgctaaag caaagaaaca aatggcgatg
      301 attgaaaagt acgcagataa acgtcaggaa ttaaaagccg caggagatcg caccgctctc
      361 gctaaattgc caagagattc aaatccgaat cgtctgaggc ttcgtgatca gacggatggt
      421 cgtccaagag ggtatatgcg aaagttcggt atgtcccgta ttaagtttag agaacttgcc
      481 catcaagggt tgattcctgg agtaaaaaaa gcaagttggt aaaaggagga aatatcatgg
      541 cagtaccagc aagaaaaaca tctaaggcga agaaacggtt aagaagaact catcaaactg
      601 tagtaaaacc agaaatttca tttgacgaag cgaatggtga ttatagacgt agccatcatg
      661 tatctttaaa agggtactat aatggaaaga aagtaattaa aggagcttca aaatagttta
      721 atatataact tttaggaggg aatcaaaatg gaaattattt acccaccttt agttgaagaa
      781 ggattaaagt attatcttga aacgacccag caatcgctag ataaatgcac tttttatcgt
      841 tcgatggtag agagaggaat tattactgaa acgggtttac cgacccaaca ggcaattgaa
      901 aatggattag tcaaagatta ctatgaggac caaggcttat catttgatga ttttttaaga
      961 atctatccaa tttttgaaga gtatgacgag gagttatttc aatgtattga tggttattgg
     1021 gagatacctg ttgatatgaa agaaaattta gtttcacaat tggaatctgg cgagttaact
     1081 tttgaagatg cacaacaaat tcaagcatat cttgaagatc gataagtatt gaaggaggaa
     1141 gatttatgcg tcaaaatatt attttggaat gtgtcgaaac tggagaacgt ctgtatctca
     1201 ccagcaaaaa taaacggaat aaccctgaac ggttagaatt aaaaaagtat tcgccaaaat
     1261 tgcgcagacg ggcaattttc aaagaagtga aatagctaag tggtaaaaag gtgacaaaat
     1321 gggcatacca gttacaatcg tttctgggtt tttaggggct ggaaaaacaa cgctaatcaa
     1381 tcaagccttg caagtctcac cctaccccag ggaagaaatt attataattg aaaatgagtt
     1441 tggtgaagtt gggattgatc acaaattatt agttcatagc gaggaacggg tgttacaact
     1501 aaataatgga tgtatgtgtt gcagtcttcg cagtgattta ttagcaatat tgatttcttt
     1561 actggaaaca atagaagaac atcaacaacc aatttcacaa atcatcattg aaactacagg
     1621 tatagcagat ccccaaccaa tcattcaaac acttttaaca gcgcctcaac taagaggaag
     1681 agtatatatc gatagtttat tgactgttat tgatagtgac agttttgaac ttattgaaag
     1741 agaaccacaa gcccttaagc aacttgtcat agcagatcgc atttttgtgt ctagaaaaca
     1801 aaacttgaat ttatctgcac taaaaaaatt acagagagaa atcaaaagta ttaacccttt
     1861 atccgatatt cgaatgttca cacatagtga aacgattcaa aaaaaggatt tttataattt
     1921 ggaaaaattc aatcatccct tagtttttga aagtgattca tcagaaaatc actataatgg
     1981 caaacactat catcaacatc atcaagaaca tgagtttaaa tcaattctct taacttcaga
     2041 tcaagacata aaaaaagatt ttttattcca attaattgac cggctgattt taaccaacca
     2101 agggagcctg tatcgtttta aaggattgat tgctttgcaa gatcaagatt ttatagttgc
     2161 catacaaggg gtaaacgaac aggttaattt tcaatacacc actcgacaaa aagaccaaac
     2221 cactattatt ctaattggaa aaatgttgga tgaagaaagg atcaaacaga gttttgataa
     2281 attactggcg gaaagcaaat aatgataagt attgattagg ggtaatttct aatggagaaa
     2341 acggatatat ctagtgcata tcgacgactt aaaagcccga atatcaaaac tagaaaaagg
     2401 gcattaaaaa ttatcaaaga acataaacaa aataaaatga agaaattagc ataattaagg
     2461 aaggaaaaag aatgaagaaa atcagtgtag gacttattgg gatagcagct ctaggattat
     2521 tgggagcttg ctccagcact aatgatgcaa aagtatcgaa tgataaagat ggaaaattag
     2581 aaattgtaac aacattctat ccaatgtatg atttcaccaa aaacatcgtc ggcgatgaag
     2641 cgaacgtgga tttaatggtt ccagcgggat cagaacctca tgactatgag ccctcagcta
     2701 aggatatggc aaaagctcat gatgctgatg tttttgtata tcataacgaa aacatggaat
     2761 catgggtacc aaaagctaaa gaaagttgga agaaagctgg gcctaatgta gtagaaggaa
     2821 caaaagacat gattttattg ccaggtagtg aagaagaaga tcatgatcac ggagaagaag
     2881 atcaccatca tgaactagat cctcatacgt gggtttcacc caaaatggca attaaggaag
     2941 tttcaaatat caaggatcag ttagttaaat tgtatccgaa aaaagcaaaa gtatttgaga
     3001 caaatgctga aaagtatttg actaaactga aacggttgga tgctgactat accactagtc
     3061 ttaaagaagc gaaacaaaaa agttttgtga cacaacatgc agcctttgga tatttagctt
     3121 tggattatgg attgatacaa gttcctattg ctggactaag tcctgaagaa gagccatctt
     3181 ctggtcgatt agctgaatta aaagagtatg ttaaaaaaaa taagatcaat tatatttact
     3241 ttgaaaagaa tgctaatgat aaaatcgcaa aaactttggc caatgaagct gggataaaat
     3301 tggaagtatt gaatcctctt gaaagtctta caaaagaaca aatggataat ggtgaagatt
     3361 atgtttcagt aatggaagac aatctaaaag cgttagagaa aacaactatg gttgctggtg
     3421 aagaggtagt tccagaaaaa gaagcgaagg atgaaaagac agttgctaat gggtacttta
     3481 aagatgccga tgtgaaggat cgcgaattga gtgattatac aggcgaatgg caatctgttt
     3541 atccgttgtt aaaagatgga attttggatg aagttttcga ttacaaagca aaactcaata
     3601 aagacatgac agctgccgag tacaaagatt attatactac tggctataag acagatattg
     3661 atactatcaa cattaaagac aacaccattg attttgtagt caacggtgaa catcatcaat
     3721 acacatacaa atatgttgga tacaaaattt tgaattatga aaaaggaaac cgtggtgttc
     3781 gttttaattt tgaaactgat gatgcaggtg ctggtcgttt taaatatatt caatttagcg
     3841 atcatggaat tgcaccaagt aaggcagagc atttccatat tttctttggt ggagaaagtc
     3901 aagaaaaact ttataatgaa atgcataatt ggcctacttt ctatccagct tcattatctg
     3961 agcatgagat tgctcaagaa atgatggccc attaaaatcc aatctttgga ggaaatacat
     4021 ggtaagaaaa gtattattgt ctatggattc caagaatatt gatgcttatc ggcgttatca
     4081 atttgcaaag caaatgagat taaatgggag tacattagct gatattgctg caactttaca
     4141 tgttagtatt gacacagttc atagagtatt aaaacgggat cctgattgtt gttttatcaa
     4201 ggtcaatact ggaagaagta gacgtttagc aaaacggcat aatagcttgg ccaattaatt
     4261 gataatacca gagtagatca tttttccctt atttggattt gatctgcttt ttattatttc
     4321 ttgaattgtc aaatcaagcg caacacttta tcaaagaaga cttcataagg tgttctccaa
     4381 tctaaacact ttctaggtct gttattcagt ttttcaatca tctgatttat cgttgagtta
     4441 tctaccgtag ctatctcttt cgattttggc aagtattctc gaatgagtct attcgtattt
     4501 taatttgttc attgttgcca agggcatgag gatctggaaa ataaaagggc atattattca
     4561 atacttctgt aactgcgaaa tgattggaga attcttttcc tctatctgga gtgatcgttt
     4621 tgaccgcctc atttggaata ctgcttaaaa gttcaatcat tttctcgctc acataagcgg
     4681 ctttctttct ggggaccttc tttcctgcta aaaggaagca tgattttctg tctgtcaaag
     4741 tcactaaaca agtaccacta gttttgccag ctacaatatc ggctttccaa tgaccaaaat
     4801 cacttctatc atctgcggat tttggatggc catggatcga atgagtaagg caaatcttcc
     4861 cacgagactc gacatgattt tttgtatgtc ttgtttttcc tcgatggtgc aatgaacgga
     4921 tcaggccttt attgccgtgt gagaggggtt ctgtatcgaa aagatcattg caaattgcac
     4981 ggtagatcgt gataaaacta attgaaaagt ccatatgttc atgcttcaaa cgattcgaga
     5041 ttttctctgg cgaccactga aaatcgacaa ataaatgacg aacaattgac caaactcacg
     5101 gatcatttag aatgtgataa cgtccgcatt tttttcttcg ataagcataa tttttttccg
     5161 ctttggaagg tgattagttt ttttcttgtt tcttctcagt tcatgtaaaa tagtaaacgg
     5221 gctatgctga atgtcgttgc caattatact aatagaaaat ccttcttcgt gaagcagaaa
     5281 gattctttct cgtttgagta tagtaagatg acaatactgg ctcataatta ttttccttct
     5341 aggagtgttc tcgacaaact tatactagtt ggatatcgat tatgactcat ttttatgtgt
     5401 tgcacttaaa gtgtaaattc aaggtataga aagaggttcc ctatgaaaaa atacctatta
     5461 caatcttaac tggctatctt ggttcaggaa aaacgatact tctaaatcat attctgtacg
     5521 gtgatcacgg aaaggaaatt gctgtcattg taaatgacat aggggaagta aatgttgatg
     5581 gggtattagt cgacaagaaa ggttttaaaa gaactggaga aaaacttgtt tcgatgccga
     5641 atggatgcat ttgttgtaca ttacgcgaag atttgattgt tgaactagat caattagtgc
     5701 aattttctga cttgaatcaa attgttattg aagcttcagg aatcagcaaa ccaattccaa
     5761 ttattcaagc aattatatta gctaaaaacc ctttagacat tgatttgacc caacgattag
     5821 agattggtgc cattattata gtagtggatg taaaacgttt ttgggataat ttcgaaacgg
     5881 gtgaaaagat tcaggaaaga aaagctgaag gtaatgtagt tgaagaagaa caggatattc
     5941 gtggcttgtt aattgatcaa ttcgagtgtt gtaacatatt acttttaaat aatagtgact
     6001 tagtgtcaac caagacgatt tacaaaatta acggtctaat tgctaagtta cagtccaatg
     6061 ccaaaataat taggacaatt aaaggtgaag tagacttatc gaaaattcta aatacaaatc
     6121 tttttaatat agatgagact tcctattcaa tgggctggtt caaagagtta gagcttggct
     6181 atcaaaatca tattcctgaa acagaagaat atggtatctc aagttttatc tatcgaaatc
     6241 ggttactcct tgacgcacaa aaatttgccc atcttacgga taaaaatttt cctggcaata
     6301 ttacaagagc aaaagggttt ctttggtttg cccaacatag tgatttttcg acgatctttg
     6361 aaacatcggg tagccataaa agtattgaat cattaaatta ttgggtagct agtttaccta
     6421 aaattgagtg tcaaatgatt atacaaaaaa acccgaattg aaggaagcct ggcatccgtt
     6481 gtttggtgaa cgcattaata aactagtttt tattggtatg aagatggatc aacaatgtat
     6541 taaggaacaa cttgagggtt gtttaactga ttctcttaat gcagataatt tccgaaaaat
     6601 ttcaccattt ccatggaaaa attctaatat gtttagccag atatcgttaa tgaaattttt
     6661 aagaaaagct ggtgtcaaga taggcgatgt cataattatt ggcattttga ttattccctc
     6721 ttttattccc ttatttgtat tctctataga aaaggagaat gaaacaactg aagatgctat
     6781 caaatatgcc atcgttcaaa ttgatggcaa ggaaatggat cgcttcgatt tggataagat
     6841 caatcataaa ctcgtgaatt atcatcctgc gaagggacaa tataatattg ttgagattaa
     6901 agatggaaga attcgagtta aagaagataa tagtcctgat caaatcgcgg ttagaactgg
     6961 atggatctca aaacccggtc aaacgagtat ttgtttaccc cataagttag ttattagtat
     7021 tgagaaaaaa gaaagcaaag attactacat ttactaatct ggaaatagat gaggaggttt
     7081 ttaataagaa aaagatatta ttattaataa tagcaggggc tttattaagt gtgttaagcg
     7141 cttgtggaca agaaaaagaa gcggtgacaa caggcacaag tatctttaac gggacctgca
     7201 atggtttagt taacagtcgg ttagattact tattttggct cttcgtcaat gagtgttggt
     7261 gaagaaattc aatagaataa tcgagatact cacacggcta gacagctatc ttgtctagcc
     7321 gtgttgttgt tctggcttta gtcagttggg tagtgttctt agttttcttg aaatattttc
     7381 ctcgatataa aaagattcgt tctcggtagt tttgaaagtt tctaaagcca taggcaattc
     7441 gttttagtac tttgatatgg ttgttcaaac attctatagg gccgttggaa tagggaactc
     7501 gcaacgctcg tttgatttct gttcgatatt tcttgaaggt cgtgaacact ggttgatagt
     7561 gctctggcaa ctccttgtag tcttctttta ggagcgcgac gaattcagga acatcttgtc
     7621 ttctgatagc ggaaagaaaa acctggtaga cttcgtagcc tcgctttaag gagtcatcat
     7681 aatataatag ccgatcgact atctccgctt ctgtcagatg atctcgaaat gacggacgcc
     7741 agtagcgatg ctgaccattc aattcccacg cattcttttg aagtagtctc cagtatttct
     7801 ttaagtgctt cgaccgacga tccccttttt taaaggtctt cattacttga attcggtggt
     7861 tggtaaaggc ccgaccgatg tgttggctaa tatgaaagcg atcggcgacg attcgggcat
     7921 tcggaaaaca ctctttcacc aatgtgcgat agggtgtata gaaatctgaa accactagac
     7981 gtacttgttc acggactttt ctagggtagc gcaagaaata gtcgcgtaaa ggattgagtt
     8041 gccgattttc cacaatatca atcagttgat gggtcttgcc gtccatcatg acaaagctca
     8101 tggctcccga gacgtttttc accgatttga attcatcgaa acacaccact tctggtaacg
     8161 gctggttcaa agaaacggtt ttaggccgaa ggctgcgaag tattcgaatg acagtggtcg
     8221 gagaaatgtg cttcatacgt gcaatcaaag acatagaaat aggctcactt aatagctcaa
     8281 gaatggcttg tttgactctg cgggcaatgg agcagcgacg atcgctaact gaatcttctg
     8341 ctaaaaaagt ctttccacag tgtttacatt tgaagcgctg tttgttaatc cgcacaatgg
     8401 tcttgtagtt actgacatca ttcaataaga tggatacttt tttccagccc catttaatga
     8461 tcgtttgctt attttgatag tggcaatgga agcattcctc tggttgatag gtcaatgtgc
     8521 catcaaaaac aaaacatgtt tcgtcattaa tgcgctcttt gcgaaaggca tcttcatgaa
     8581 aggtaagatt taggtctaga atatctagta actctttggt aaactctgaa taggacatct
     8641 tgaaacactc ctttgaatgg ttttcgtcga ctttattcta aaggatttca aggtgtctgt
     8701 ttgcgtttat tagaaaaaag aaaaagtatt ggcaacagaa ggcgtcacca acactagata
     8761 ttatagatcc cttattttct tcgcttcaaa ttgtcaagga gtcatcataa attttgtatg
     8821 gattcttttg tttgggtagt agtcttctaa ataatcttgg attaacacct tcatctctat
     8881 tttactttta tatttgttgg agtcgattaa ttctcatttg attgatcgat gaaacgcttc
     8941 aattactgca ttgtcaacag gagtcccagt ttggctcatt gaatgtgtga tgtggtggtc
     9001 ggtcaacagc tcttgataaa taaaagaccg atgctggcta cctctatcgg aatgaattac
     9061 cgtaggcaat tctccttaat aaatttgtaa tggacggacg acaatctctt ttttcatgtg
     9121 tgaatccacc atatagtaac gtagaatacg agttgctaag tcaatatagg tggacaaata
     9181 gaggcgaccg tcattacatg gaatgtaagt aatatccgtc acccaaacag catcaatttg
     9241 gttctgttca aattatcgat tcaacatttt ttttaccatt tctttgattg ttttttctct
     9301 accatgagaa gatttgcgtc gatgaaatcc tcttgtgcat aaattcattt cacgcataag
     9361 aacagctaca acacgtttat tgatttcgat tccttcatcg taaagttttt gagtgatccc
     9421 tggagatccg tatcgatctt tgtgttcgta gaacactcgt cttcctcttt tagtcgttta
     9481 ttttcctgtt caagaatgta gaaatctgct ttatcaggta atctttttcc ttgtccagca
     9541 aaagcatttt tcccgtatcg acgatattct gaaacccatc gggaaattgt tggacgagag
     9601 atattgtatt cttcagcaat tttcttggta gttacttttt tctcaaggat atcctgtgaa
     9661 atctttagtt taaatttacg atcatatgtt tttttcaata gagttcattc cttccggtaa
     9721 cgtaactgat agtcaagaaa agtgtatcat agcccaactt agtatcaggg tttgtcaact
     9781 aaactgtgga agttgataga taagagtttt taacaaccaa aattctcttg actatttttg
     9841 aaatcagaca aattttgatt ggacacatag ttgaataaat ctaccgtcgt tacggaaggt
     9901 aaatcgcatg cttttcaatt ctagaggaga agtcctcgtt gaagtaccat ggcccagttc
     9961 tggattcgac ctcctgccca cttcgtacgt aaataaagta attttaggag tgagactgtg
    10021 acaaaagtta tttttaagct aaaaacgaat taaaaaacgt taataattaa aatcattcgt
    10081 tttagattct atgaatcatt atagaacgct gtaaaatc
//
LOCUS       DAYYGO010000098         9924 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000098 DAYYGO010000000
VERSION     DAYYGO010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.100"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(390..614)
                     /locus_tag="ACHA5T_002189"
     CDS             complement(390..614)
                     /locus_tag="ACHA5T_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887743.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            805..3582
                     /locus_tag="ACHA5T_002190"
     CDS             805..3582
                     /locus_tag="ACHA5T_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8887744.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            3663..4082
                     /locus_tag="ACHA5T_002191"
     CDS             3663..4082
                     /locus_tag="ACHA5T_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8887745.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4094..4564
                     /locus_tag="ACHA5T_002192"
     CDS             4094..4564
                     /locus_tag="ACHA5T_002192"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8887746.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4581..5360
                     /locus_tag="ACHA5T_002193"
     CDS             4581..5360
                     /locus_tag="ACHA5T_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8887747.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5347..6168
                     /locus_tag="ACHA5T_002194"
     CDS             5347..6168
                     /locus_tag="ACHA5T_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8887748.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6185..7234
                     /locus_tag="ACHA5T_002195"
     CDS             6185..7234
                     /locus_tag="ACHA5T_002195"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8887749.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7247..8251
                     /locus_tag="ACHA5T_002196"
     CDS             7247..8251
                     /locus_tag="ACHA5T_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8887750.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            8884..9903
                     /locus_tag="ACHA5T_002197"
     CDS             8884..9903
                     /locus_tag="ACHA5T_002197"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFM8887751.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3329 a         1645 c         2074 g         2876 t
ORIGIN      
        1 gaaaaatatt tattcgttta ttgtgggtac tatcttagag gaataaataa tagtggagaa
       61 aactatggtt tggcgcagat tctatcaggt ttgatggaac gaagacaacc tcccgacatc
      121 aagtcaaaag ccacttgaga ttttgatctg aaaataaaaa aagaaaacaa ggtaggaaca
      181 tcaatgtttc taccttgttt tcgtaatcaa tgggaaaaaa ctaagagtgg tgtacgtaat
      241 caataaaaag gatggtgaca acagtacctc tttactctct gaaagcatca taactcgttt
      301 ttcctcatta tgccaataaa ttgttgttat accacatata aggatttgta atagctaaaa
      361 acagacagga aagttgaaat ctgtgaatct tattttgtat ggaaggattt atagaacgca
      421 aaaatcaaga taaagatgaa taaaaatcct gtttcggcta tatattgcgt atctacgcca
      481 ataaaacgag tttgttctgt catagccata taaagcaaag gcaaaaatac aactacagcg
      541 agataagatc cgatgatttc tcgaaaagtt ttccaggaag tatgtttcct gtttttatat
      601 ttatccgatt tcattgaata gtcatagcct ccttattctt tcatatgttg aaattccagt
      661 ataaaaactg gaaggaactg tacgtttttt agaataaaaa ttataaggga taagtaggct
      721 gctgtctata ataagatata tttttcaatt ctttattaaa gattttattg acaaaatgaa
      781 tccgttttct atgatggagg aggggtgaat aagttgttga agaaggaaat tttatgttac
      841 ttagagaacc agacagcctt tttcgattta gaacatatga acgaggtctt tacagcacaa
      901 aggttagctg atcattttgg ggtaaaaaga aacacagtca gtcattattt gaaccagtta
      961 aatgatgagc aactactagt caaaatcaat tctcgaccag tgatttattt ccacaaagaa
     1021 gcttttgaaa aacaattttt taagttacgc acgaattttt atttttctat cgatgatctc
     1081 aaagaggaac agcctttttt cgcgcaacca aaagacttat tctcttttat gatcggatac
     1141 gacgccagtt tgaaagaaag tatcgaacaa atcaaaacag ctctttacta tccagatggc
     1201 ggattaccac ttttgattac aggcgaaagt ggcacaggta aaagttattt agttaatttg
     1261 gtctatcagt attgtctgct tcatgatctg ctagaagatt ctgcaccatt catcactttt
     1321 aattgtgcac aatatgcaga taatcctgaa cttttgacta gcaatttgtt tggacatgtt
     1381 aagggagcgt acactggtgc agaagaaaat aaaaaagggg cttttgaagc agcagatggt
     1441 gggatcttgt ttttagacga ggtacatcgc ttaacaccag aaggtcagga aaaactgttc
     1501 acctatcttg atcaaggtgt gatttatcgt atgggagaac ctaatttgtc acgccggatc
     1561 aaaacacgtc tcttttttgc tacgactgaa gagattacta gtcatttctt aacaactttc
     1621 attcgaagaa tacctatccg aatcgaacta cctgcactta atcagcgtag tcgtaatgaa
     1681 cggctagaac tgatttactc tttttttctc gaagaacaaa gaaaaatggg gagccctttg
     1741 tcagtcagtg gaccagcttt gtcactttta acaagtcaga aattatccgg aaacatagga
     1801 gagctgaaaa atatcgtcaa agtttcagca gcaaaagcat atgctgaaca gcgtgaacag
     1861 cctgaaatcc atgtcacgat ccatcatttg caaaaagaac tacttgcgca gcctattact
     1921 aaaaatacag cacagcaagg aatctatatt acgcaagacc agaccttaga gcaattaatg
     1981 gagaagcaac agcccgaaca gcagcgaatc gttaaaagtt ttgagcaaat cctaattgat
     2041 ttcaagaaaa ataactgtct gctatattct tgcgaaggaa aattaaagca agaagtgatt
     2101 caattatttg attttttgct gtttgagacc agtagacaag aaaaacacga gctgctggtt
     2161 tattggacac aatctatccg agagaccttt cgacaaatgg aaactgccta tcaaatcaaa
     2221 ttcgatggaa acagtgtcta tgccttgagt tactatctct atcagcgaag cactatcaaa
     2281 tggttaccgg aagaacatga tattatccaa ctaatcaaag aattagaaca acaagtttct
     2341 cagtcttatc catctagtta tcactatgtc caacgaattc tggaattaag taagatcaaa
     2401 ttagatattg aagacacaag tatggatcgg attttactga cgatctatct aaaaaaagcg
     2461 aaatggtcaa aagaaaaacg actgccaaaa gcgatgattg ctgctcacgg ttatgcaact
     2521 gctagcagta tggcaaatgt agtaaatcgt ttattacaag aggatctatt tgaatcattt
     2581 gatatgccac tagatgtcac accacagcag attgcttcag aaatccttga ttattgcgaa
     2641 aacagcgatg tttcaaatgg attgatcata ttagtagaca tgggatctct gaaagaaatc
     2701 catcagtttt ttaaaaaaca gttatccgta ccgcttttga ttttgaataa tgtgacgaca
     2761 cctttagcaa tcacagtagg ggaatgcctg caaaaaaatg tatcattaga agagatagca
     2821 gaagagactg ttcgacagat ccagccagaa tggcgactgc tctatccaga ggaaaacaaa
     2881 cccaaagcac tcattactac ttgcttcact ggaatcggta cggcggtcca cttaagtgaa
     2941 ctgctagaaa aaagcttacc tacggcttgt cagttgaaaa tcatcccata tgaatatcag
     3001 caattaaaag ataagaaaaa cagtgaccca ttattttcca tatacgaagt cgtaggaatg
     3061 attggcacca cagatcctga tatcaccaat atcgactatc tttcattaga agacctcatc
     3121 tcgggggaaa agatgtctgt actagctgaa tggttggaca ctactatgaa tgcttcagag
     3181 aaggaaatat tcaatcagcg catcattcgt aatttttcat tggaaaaagt actggattcc
     3241 gtgactattc tagatacaga gaaggtgatg ggagaaatcg atcattttat gagagaatta
     3301 gaggtgcagt tgaatcgaag catcagcaat cccaaaaaat tagcgttgta tgtccatgtg
     3361 agctgtctca ttgaacgatt gatccgacaa atgccaatcg aaacctatca aggattagag
     3421 aaatggaagc agtgtcaaaa agaagggtta tctgctatta aatctgcgtt tagtgtcatt
     3481 gaagagcatt atagtgtcat gatccccaat tctgagattg cctatatcta tgatattttg
     3541 agtgaagata cagagtttct ctcgatagaa gaagaatttt gaacataagc aagttgttgc
     3601 cactaatttt ggcatgcaac ttgctttttt agtatgtgta agtgaaaaga ggagatgaaa
     3661 aagtggatag agaaattatc ttagcttccc atggaaaatt tgcttcaggg atattagatt
     3721 cattagagct gatctatggg aaaaatcatt ctattactgt attcgattgc tatactgatg
     3781 agaattttga tttagcggaa acagtaaggc agctttttat gaggtacaaa gataaagaaa
     3841 tcattgttct gacggatctt tttggcggaa gtgtcaacaa cgaattctta caatatatca
     3901 atcgggatca tgtctatctc gttgccggat tgaatttgcc actattgatt gagctagtat
     3961 ctcggttgga cgctgaagta gagacaactg ttttgatcca gcaggtgcta actgattcaa
     4021 aaaaaatgat ccagttttgt aacgagcggc tcaatcagaa aatagaagaa gacgaatttt
     4081 aggaggaaga aaaatgattg tattgacaag agtcgaccat cggttattac acggacaagt
     4141 agctttttca tggacacaaa caataggcgc agactgtatt ttgattgcta atgatgatgt
     4201 acctacgaat gagatacgaa agacaacgat caaattagcc aagccacaag gagtcaaact
     4261 agtgatcaaa tcaattgatg actcgattgc cgcgttgaaa agcggggtga cagataaata
     4321 taagttattc atcgtagtgg aatcgatcga ggatgcatat aagctggcag aagcgtatcc
     4381 agaaatcaaa accatcaact taggagggat caaggcaaaa cctggtactc gcagcatcgg
     4441 aaaagcggta aatattttac cagaagagga ggaacgcttg aaacgattaa ttgacaaaca
     4501 aatagaagta gaaatccgtc aagtaccagc agataaaaaa gtggatgtaa gagaagtttt
     4561 gtaagaaagg aagaacctca atgattgtac aagcagtctt attaggaatt gttgcatttg
     4621 tcgcacaatc agaatatgca ctagggacat cgttgctttc tcgtccgatt gtgacggggc
     4681 tgtttacagg aatcgtgtta ggtgatgtaa aaactgggat cattatgggc gcaactttag
     4741 agttagcatt tatcggatcg ttctcagtag gtgcttcgat tccacctgat gttgtgaccg
     4801 gtgggatttt agggacggct tttgctatta cagctggagc cggtacagaa accgcgcttt
     4861 tactaggttt gccgattgct acgttgacat tgatattgaa aaatatttac ttagggcttt
     4921 tgattccaat tatgaaccat aaagcagaca attatgcaga agaaggaaat tataaaggag
     4981 tcgagcatat gcacttgctt gccggtatcg gtctttcctt gatgcttggt gttgtggtaa
     5041 tggtttctta tatggtggga agcaatacga tcggaaatct attaaatatg attcctgact
     5101 tcgttcaaaa ggggttgtca gtagctacag ggttgattcc agctcttggt tttgcgatgc
     5161 ttgctcgttt gctgatcaac aaacaagttg caccatattt tttcttaggt tttgctatag
     5221 cttcttactt agagattcct gtgaccggag tagctatttt tggtgcgatt ttagcagttg
     5281 tggtagtcaa tattatgaat gtccgtcaac taccacttca aacaactgca ggggaggtag
     5341 aagaagatga agaattctga gatcaaaaac agcaaaataa cgaaaaaaga gctaaatcac
     5401 gtattttggc gttcttttca aatggagttt tcttggaatt atgaacgtca gatgaatatg
     5461 gcttatgttt atgcaatgat tccgattttg aaaaagttat atcagtcgaa agaagaaatg
     5521 gcagcagctt tgaaacggca tctagagttt tttaacacga ccccacatat cgttacactg
     5581 attttaggaa tcacggctgc aatggaggaa gaaaataagg atgatcctga atttgatgtg
     5641 actgcgatcg atagtatcaa aacatctttg atgggacctc ttgcagggtt aggagattct
     5701 ttcttttggg gaactttgcg attgattgcc acaggtgtgg gtacctcttt agccatgcaa
     5761 ggaaatattt taggccctat cttgttcatt ttgattttca atattccgca cattctgttt
     5821 cgctatctag caacaggttg gggatatcgc ttaggaacgg gttttttgaa aaagatccag
     5881 gcaaacggta tgatggaaag tttgacatta ggcgcgtcta ttattggact gatggtcgtt
     5941 ggagcaatga ctgccacgat gatcaccatc aatattcctt taaaaatcgg ttcaggagaa
     6001 aatgctgtga ctgttcaaag tatttttgat gatatcgttc caaatatctt atgtcttgga
     6061 gcatttggca tagtgtttta tctattgaag aaagaagtga aaccattaac gatcttgatc
     6121 ggactagctt tctttggaat ttttggttcg tggataggga ttttttaaaa aaggagcaga
     6181 gaaaatgttt acgatgcaag attatatcta tgaagaaaag gaagtactaa gtacgatctt
     6241 gaaaaagaat gatttttcga caagagaaca tatgaaaaaa acagtgaatt tactgatctt
     6301 agcaacgggt tcatcttata acgcttgttt agcagcgaag ccagcattgg aatcttatgg
     6361 tgatcttact gtcgatattc aagaaccttt ttattttttc aattatggaa aactttctcc
     6421 ttcaatcgat actgtgattg ctgtgtcaca aagcgggaaa agtgcttcta ctgttgaagc
     6481 agtaaaaatg atacaaaaac aagggttacc tgttgttgct atcacaaatg acgtgcaaag
     6541 tccacttgct ttagaagctg cacaaattat tgatttaggt gccggggtag aatcagtagg
     6601 cttcgttaca aaaggctatt ctgcgactgt tttacagtta ctactcttag ggataggaat
     6661 tggaataagt aaaaataaaa tcagcaaaaa aatagaacaa gactacatgc aacagttgcg
     6721 caaaataatc aaccatttac cagcgattat tcaaaaaacg gaagaatttt ttgatggata
     6781 tcaaagttta tttcgtttag cacagcgttt cgtcaccatt ggatatggtc caaactgggg
     6841 aacagctaaa gaagcagaga cgaaattgac tgaaacaatc cgcgttccgt cacaaggatt
     6901 tgagttagaa gcttatatgc acggcccata cctggaggca gatgcttcgc atttgctatt
     6961 ctttattgaa ggggaaagtg taaataagga acgttcgcaa aagttgcaaa gatatatgag
     7021 tcggtacgtt ggagagactc tcaccattac cacaaaaaaa gcaagaaacg agaaaacact
     7081 tggattagca atagaatgtg atgaatatct gtctgtatta gcgcttgtcg ttccgtttca
     7141 actattcgct tataaaaccg cagtagctaa aggtattgac ttgaacaaaa agatttttga
     7201 agactttgat acagtcttga aaagtaaatt ataagaggtg gaaatcatgc tgaaattcaa
     7261 tgaagaagaa caaatcaaag caataaaagg cgcactagct ttacgtccgc aagtagaaga
     7321 aatcatcgat aaactttatg cggaaaaatt tgacgctgtc tattatctag gaatcggtgg
     7381 aacgtatgct tcctcgatgc aagctgtcac ttatatgaat gggaaaagca acttgcctgt
     7441 ctttgtacag catgctgcag aatattacac tactggaaac aaaagattaa caaaagattc
     7501 tatcgttatt ttgtcctctg ttaccggtac aactcaagaa gtagtgaaag cagtagaaca
     7561 aatcaagaaa gtaggagcga cactgattgg atttatcgat aaagcgaaca gtaagttgtc
     7621 tcagctatgc gattttgtgg tcacttatcc agctcctgga acagaacaaa tcaaattttt
     7681 tatggcagca gatcgattga tgtatttgaa tggagaattc gaagcttatt cagaatacta
     7741 tgaacagctc gaacgttatt taccaacagg gttagtggaa gcagagaaaa aagcagatgc
     7801 atttggctta tcatttgcag aaaaacatcg acgtgatagt atgcattatt ttattggagc
     7861 agggaatcaa tggggagctg tttattcata tgccatgtgt tactgggaag aacaaagctg
     7921 gcttccatcg aaatctatcc atgctgccga gtttcttcac gggacgttag agatcgtaga
     7981 agaaacaacc ccagtcacat tatttttagg cgaagatgaa cagagagtgt tatctgaacg
     8041 tgtggctaaa cttttgccaa aaatttgcag caattataca ttgatagaca caaaagacta
     8101 tccagtagaa ggtatcagtg aaaaatatcg cggacgtgtt ttgtctttct tattgatgca
     8161 tgtagtgaca caacgcatcg atgctcatgt ggagcagttg aactgccatc cattagagat
     8221 acgccgctac tatcgtcagt ttgattatta ggatagactg gataaaaaga atataaaaaa
     8281 taaagagagc agcgataact gctcccttta tttttttaaa atattggcat caattacgtt
     8341 ataaaacaca ttttttgcat tatctacagt tcaacttata atctgtttaa tgggacatgt
     8401 gggtgccaac cattttttgt tatgcaatag tactatttag ttgttgcatg gatccgtagt
     8461 aaaagtccaa gtacaacgag ttgcgccagt ttacatagcg ttttgtatcc agaatgttga
     8521 ctaaaacaaa agttcctatg tcaaccgtca acagagacaa tctgtccgtt gcaggacctg
     8581 acagatttag aaatgttcaa tctgtctctt atgtatttta aatcaatact cactaggaga
     8641 agtttcctaa cctcctaata gtaatgctgg tagtaacatt gtcaaaactg cttcctctta
     8701 ggaacaagca aagcataacg agtgtaaaaa taaaatgtag actattgtaa catatcattg
     8761 attatttcaa gtaaaaacga taaaagaagt aaacaaacac ttaaaaacaa tggaaattag
     8821 tcccaacttt aagaaatgta tactataatg gaaattatca attatttatt tggaggaata
     8881 tccatgaaac aaaaaatcgc agttcttggt cctggatctt ggggcactgc tttagcacaa
     8941 acattggcag aaaacgggca tgatgtccgt atttggggaa atgtacctga acagattgat
     9001 gaaatcaata agtatcatac aaatcagcat tttttacctg atttgacgat cccagaatcg
     9061 attatcggat ataaagaatt ggcagaagca gtggataacg cagatgctat tttatttgtt
     9121 gttccaacaa aagcgattcg ttcagtcgca caggaactta ttggtaaaat gaatactaag
     9181 ccaatcatta tacatgcaag taaaggacta gaacaagata cgcataaacg aatctcagaa
     9241 gttttggctg aagaaattcc agaagaaaaa agacaggcaa ttgttgtatt atctggtcct
     9301 agccatgctg aggaagtggc agtacatgat atcacaacaa ttacagctgc aagtattgat
     9361 caaaaagcag cagcctatgt acaaaaactg ttcatgaatg attatttcag gatttataca
     9421 aataatgatg tgattggcgt agaaactggt gcagcactga aaaatatcat tgcaattggt
     9481 gccggcgcta tccatggatt aggattcgga gatgatgcaa aagcagcaat catgactcga
     9541 ggattggcag aaatcagccg tcttggtgtc gcgatgggtg ccaatcctct gacatttatc
     9601 ggtttgagtg gtgtggggga cttgatcgtt acttgtacaa gtgttcattc tcgcaattgg
     9661 cgtgccggta acttgttagg acagggacat aagctggaag aagttctaga aaacatggga
     9721 atggtcgttg agggtgtggc aacgacaaaa gcagcagtag agctagctca acagctaggt
     9781 gttgagatgc caattactca aacaatctat aatgttttgt ataatggaga agatatcaaa
     9841 aaagcagcaa aagaaatcat gttacgtgac ggaaaaatgg agaatgaatt ttctcttcgt
     9901 taaatagatg atcaatagtt aatc
//
LOCUS       DAYYGO010000099         9902 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000099 DAYYGO010000000
VERSION     DAYYGO010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.101"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            96..604
                     /locus_tag="ACHA5T_002198"
                     /pseudo
     CDS             96..604
                     /locus_tag="ACHA5T_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
     gene            697..1859
                     /locus_tag="ACHA5T_002199"
     CDS             join(697..973,973..1859)
                     /locus_tag="ACHA5T_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8887752.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2139..3119
                     /locus_tag="ACHA5T_002200"
     CDS             2139..3119
                     /locus_tag="ACHA5T_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8887753.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3241..4659
                     /locus_tag="ACHA5T_002201"
     CDS             3241..4659
                     /locus_tag="ACHA5T_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFM8887754.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4730..6199
                     /locus_tag="ACHA5T_002202"
     CDS             4730..6199
                     /locus_tag="ACHA5T_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8887755.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6209..6676
                     /locus_tag="ACHA5T_002203"
     CDS             6209..6676
                     /locus_tag="ACHA5T_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFM8887756.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6712..7584
                     /gene="scrK"
                     /locus_tag="ACHA5T_002204"
     CDS             6712..7584
                     /gene="scrK"
                     /locus_tag="ACHA5T_002204"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFM8887757.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFIKSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7665..>9902
                     /locus_tag="ACHA5T_002205"
     CDS             7665..>9902
                     /locus_tag="ACHA5T_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8887758.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDS"
BASE COUNT         3436 a         1435 c         1980 g         3051 t
ORIGIN      
        1 gctttttatt tattttttct tataaatttg ataaatagaa attagtatag ccctaaacaa
       61 attatttgtg ttttattgtt tagggctata ctatattggt acaaggaata gaaagaggtg
      121 tttatgtggt tgataaaatt cagtttaatc aagttataga agaattcatc aaagtaaata
      181 atttaatgac ccatattcaa aaacgaccaa tcagaataac taatgatcta aaactcagta
      241 cgagtatgat tcatttgatt gatgtgattg gaaattatcc tgaaactagt attacggaat
      301 tagctgatcg attaggcgtt accaaaggag ctgtatccca acagattccc actttagaga
      361 aaatgaatct tataagcatt tctcaaaaaa aggaaataag aaaactaaac ttgtatcgtt
      421 aacaaaatat ggtcgaaagg tgtacgaatc acataattat ttacatgaag agctttatag
      481 ctcaataaat gaagcgttag aatcattctc accagaccaa atttcaacca ttaaacagat
      541 tctagaaaaa gtatcttata caatcaatga atatcaagta agtctgacaa gaaaggaaga
      601 ttgacatgaa aaaaatgatt tttgaatcgg ctacactaga ctagacagaa aaaataaggt
      661 gtgtaaaata aatgaaaaca cactggagga atttttatgt caagacgtca acgtagaacc
      721 tattcaaaag aattcaaaca acaagtcgtc gatctttatc tcgcaggaaa gccccgagct
      781 gaaatcattc gagattatga gctgactacc tcggcctttg ataagtgggt caagcaagcg
      841 caaactactg gctcgtttca ggaaaaagat aacttgatcc ctgaacaagt agaactaatt
      901 gcattacgaa agaaaaataa acagcttgaa atggagaatg acattttaaa gcaagcggcg
      961 ctgatattgg gacgaaaaga caagtaattg ataccaataa gcacaaatac tcaatatcag
     1021 cgatgtgtaa agtgttaaac atctcttgcc aaacctatta ttatcaagcc aaaccagttg
     1081 aagatgaatc agaactggaa gaactcgttc aagaagaatt tattcgcaat cgaaaagctt
     1141 atggtaccag gaagctgaag cagtgcttgg ccaatctagg tcttcaagtg agtcaacgca
     1201 gaatcggccg tatgatgaaa cagcgtggct tgcgttcaac atacacattg gctcatttca
     1261 aattccattc ttctacttgt aacgaagcca agaccgccaa tattctagac ggaaccttcc
     1321 tacaggaaaa gtcattagaa gcgattgtaa ccgaccttac ctatgttcgt gttgggaaga
     1381 aatggcagta tatctgcctg attcttgatt tgtttaatcg agaaatcatc ggctattcat
     1441 gcggggagaa gaaagatgcg acgttagtca aggaagcttt tgcaaagatt ccgtactcgt
     1501 tgaataatgt acaacttttt cacacggatc gtggaaaaga attcgataac cagacgattg
     1561 acgaaatttt atgggccttt aatatcacgc gttccttaag caaaaaaggc tgcccctatg
     1621 ataacgccgt tgcagaatcc acctataaat ctgtcaaagt agaattcgtt catcaatacc
     1681 gctttgagac attggctaat ttgcgattag agctatttga ttacgtccat tggtggaata
     1741 ccatccggct gcacggcacg ttaggctatg agacaccgat cagttttcga cagcagagat
     1801 tggcgcagcg aatccttgat aatgagctcg gatgtgatac cggtggcgag gcaccttaat
     1861 tatttgaatg agcttctgcc ttagagaatc agattcgagg aggctcgttg tcaagggcag
     1921 tcggaacacc agcagcaagc ttacgcaata ccttataatt tttgtcaaaa aaagtgttgc
     1981 cattccacat atatctttaa acagctattg ccagataaaa ataaatgata ttatatatat
     2041 aaatacttat ccgaaaaggg gaatgagaga gtcaaggttc ttggaatgtc gaatagtcga
     2101 gtaggtagat tttctatcta caaccttgtt gtttaaaaat gaaaaaaatt ggaattagag
     2161 aagttgcgaa agaggcaaat gtttctccta cgactgtgtc gagggtattg aatgatcgtg
     2221 gatatttgag tgaggaaaca aaaagaaaag tttttgatgc catgaaaaaa ttagaatact
     2281 atcctaatga actggcgaga gcacttttta aaaacgaaac atataccgta ggtttgattt
     2341 ttcctacaat tactaatcca tttcacgctg agttaattca agatatcgag ttcgagcttt
     2401 ccagcagggg atataaagtg ttgctgtgca atagcttaaa caatccaaca aaagaaaaag
     2461 agtacttgac gatgttgaga aagaatcagg ttgatggaat tatagtagga acgcacaata
     2521 aagatattgc ggattatgat attccagggc tacctattgt agcgattgat agaagtcttg
     2581 gtgaaaatac agttacagta tcttgtgata attacagggg tggggaatta gctactcaac
     2641 tacttgtaaa tcatggctgt cagaatatac tgtgtattcg gggagatagt aagataaagc
     2701 tacccgcgaa tagtaggact ttagcatata aagatgtttt gaataaattc atgctggaac
     2761 cgttagtcct agaagtacca tttgtaaaag aaatcaatga aaaaaatgaa ataattaaga
     2821 catatctgga aactcatcca aatattgacg gggtttttgc aggagatgat ttattggctt
     2881 ctttggcgat taacaactta ggaagttcgg gcaaaagagt tccaaaagag gttaaagtga
     2941 ttggatttga tggagctcaa caaacattga tttataatcc agaattatca actattcaac
     3001 agcctataga tcaaatatct aaagtagcag taaaaaaatt attgaatatg attaagggcg
     3061 aaaaggagac ggatgaactt tcacttccag taaagctcat aaaaaaaaat acagcataag
     3121 ttaaatcggc gaaaaattcc ttcgtcgatt taaaaatatt tttatgaaac gggttgacat
     3181 atgaaacggg ttgacatata ttagttgtga agataaaaac cttttcactg gaggaagaaa
     3241 atggagaaat tattaattaa aagcaataat catcaacttt actaccaacc taaggatgtt
     3301 tgggtcggag atgttatgcc ttacgggaaa gaaggaaaat tctttattta tcatcaacgt
     3361 gatactagaa atccagttcc ttttggagaa ccgtttggct gggctttagc aactacgaaa
     3421 gattttgttc attttgaaga ctttggggaa tctcttttaa gaggggatga caatgagaga
     3481 gaccagttta tctacgctgg ttctgtattt gaggcaaatg gaaaggttca cgcattttat
     3541 acagggtata atcgtgaatt cttagcgcag ggaaagacat ctcaagtgtt gcttcatgct
     3601 gagagtgatg attttgttca ttgggaaaaa tcagaaacag cattggagtt agaaccacaa
     3661 gaagggtatg atattcgaaa ctggagagat ccatttgtcg tttgggatga tgaaaaagaa
     3721 gaatatttgc taattttagg ggcgcgaaag ggtcaggata aacatctaca aaccggacgt
     3781 ttagtggaat ttacatcaaa agatttaact aattgggaat tcaaaggtga tttttgggct
     3841 ccgggattgt ataccatgtt tgaaatgcca gaacttttca agattggtga ctggtggtat
     3901 cttgtctatt cagaatactc agatggaaat aaaatactct atcgaatgag tcaaagtttg
     3961 gaaggtcctt ggattaagcc taaagatgaa gcttttgatg gacgggctta ttatgcaggt
     4021 agaacagctt atgatggaaa acgtcgagtc ctatttggat gggtaccaac aaaagatcaa
     4081 gaaaatgata aaaacaatta tttatgggga ggtactttgg ttcctcatga agtctatcaa
     4141 aaagaggatt tttcacttgg ggtaaaaccc atagacgaag tgtggaatgc attcataaat
     4201 ttacatgata ttgccgatgt agagatagaa acagcagaca caagagaaga agtaacgctt
     4261 gtcaaaaata gtggaacttt gtttaattta gaaacaacaa tttcatttga tgagaatctt
     4321 agtgactttt cactgagatt atataaaaac cttgagacag atgaatctta cgaatatcgc
     4381 ttcaatttag atgaggggcg tcttacattt gataaaaacc cttgttaccc ttggtttcaa
     4441 tatatggata aaggacttat tagacctata tctttaaagc ctaaccaatc ttataaacta
     4501 aaagtgattg ttgatgacaa tataatgaca ctttatattg atggaatcgc tcttaacgct
     4561 cgtgtacatg gttgttttgg tcaagcaatt actattacag caacaaatgg atcaatgact
     4621 gcaataaata ccaagttttc aaatgaaata aagaaataat aaacgtcaat catgtggcag
     4681 gtctgaaaat caaacatgac ctgtcacatt taaaagggag gataaatgaa tggactacaa
     4741 aaaaattgcc gaagatgtat tggaaaacgt tggaggaaaa gagaatatct caagcgctac
     4801 acactgcatt actcgtttac gtttaattct taaagacaaa agcaaagcga atatggaaat
     4861 gcttgaggaa atagaaggtg caaaaggtgt tcttttcaat agtggacagt tacaagtaat
     4921 tttcggaaca ggtataattg aaaaggttta caaagagttc gttgaattga caggagctaa
     4981 ggaagtatcc gtttctgaaa tgaaagacga aggagtcaat aattcaggaa atagacttca
     5041 acgagctttt aaagttttct cggatatctt tattccgatc attccagctt ttattggggc
     5101 agcaatggtt ttaggaattc gttcgttgat taccactcct ggaatgtttg gaatggatgg
     5161 ctcattcgct gatcacaatg tattcctaaa tgatttatcc aaatttctgg gtattattgc
     5221 aaccacattt gattatttac cagttttagt tatgtattct gcaacaaaac gttttggcgg
     5281 aaaccctatt ttaggattac ttgtaggatt tgttatgatt catccaggac tagccaatcg
     5341 gaatgatttc gtcatgggaa ctataaagcc tgattattgg catttatttg gattaagcat
     5401 tccagcagtt gcgtttcaag ggggtgtttt tccagcaatt ttgacttctt ggtttttagc
     5461 aaaagttgag aaaattacaa ataaatattc tcctcaagta cttgcttaca ttttagtccc
     5521 aactgtgaca attgttcttg ccaactttgc actattttta gtgtttggtc cattggggaa
     5581 ttttatcggt aatgttttag gtggaatcat tgatttccta tatatgaaga tgggtgcctt
     5641 tggtgctttt gtttttgcag gcgcgttaca accattagtt gtaacaggaa cacaacatgc
     5701 tattcaaggt attgaggcaa acttagtcgc aacaactgga ttcaattata tccaacctat
     5761 ttggtcagtt tcaattattg cccaaggtgg cggttgtatt ggtatgtatt tactctttaa
     5821 gaaaaaatct aaagatagag atattgcgat ttcaagcttt attccaacac tggtaggtat
     5881 tactgaaccg gctatctttg ctgttaattt gaaatattca atcgttcctt ttgtttgctc
     5941 gtttattggt gctggttttg gtggagcatt tatgaaaatt tttgatgtaa aagccattgg
     6001 acaaggattg actgtattgc caggtctgac tattgctaat ccagttcttc cttatatatt
     6061 tggtaatctc atagcattca ttttaccaat catttttatt ctcgcttatg ataaatataa
     6121 agtgtttatc cctgaagcta aacaccgtaa atttgcagaa aaaactgact caaaaaaatt
     6181 gccagttcaa gtaaagtgag atgagagcat gttaaatttt tttagaaaaa atgaaataag
     6241 cgttgtagca cctgcaaccg gtgtttttga aaaactggag aaattaagtg atccagtctt
     6301 ttcaaaagga atgatgggac aaggatttgc aattgatgca aaaaacgaaa cgattgtatc
     6361 tccaatcgat ggttttgttt caagtatttt tcctacaaaa catgccttag gaataaaaac
     6421 aaaatcagga attgagatac ttgttcatgt aggaatagat acagttgagc taaatggtga
     6481 gggatttgat attaaagttc aagaaggaca aaaagttaag gctggggata ttcttatgac
     6541 cattaatttt gtagtttttg aaaagaatca caagactaaa gatgtcattg ttgtttttcc
     6601 agactaccaa gacgaaattt tcattccgaa acttgaagaa gtaaaacaag gacaagaatt
     6661 gtttgttctt ggctaatgat aagtaattaa taatagaatc gaggaaaatg tatggttttt
     6721 ggggctattg aagctggtgg aacaaaattt gtttgtgcca ttggagataa agatttgaac
     6781 atatttgaaa aagtcagctt tcccacgact acacccaaag aaacaatgaa tttagtttta
     6841 gattttttta atcatcataa agatgattta gaaggtatcg gagtgggatc gttcggtcct
     6901 attgatattc attgtgaatc taaaacgtat ggcttcatta aatcaacgcc aaaacttgct
     6961 tggcaggatt ttgattttat tggaagttta aaaaagcaac ttaatattcc aactgcttgg
     7021 acgactgatg taaatgcctc ttgctatggt gaatatgttt ctggaaatgg cagaggcctt
     7081 tcaagtgttg tttactatac cgtaggaacg ggcataggag gtggcgctat ccaaaacgga
     7141 aacttcattg aaggttttag tcatccagaa atgggacata tgcttgtaaa acgacacaat
     7201 aaagacaact tttctgggac atgtccattc cacggagact gtttagaggg cttagctgct
     7261 ggtccggcaa tcgaaagaag atcagggaaa aagggacaag aagtgactga aaatgacgtt
     7321 acttgggaaa tagaagctta ttacattgct cagtgtgcat ataatacaac attgatgtta
     7381 tcacctgatg tcattatatt tggtggcgga gtaatgaaac aaaaacactt aaaaaagaaa
     7441 gtacaagata agtttattga gttgatcaac ggatatgtcg ctattcccga tgttgacaat
     7501 tatattatga caccaaaatt gttagataat tctggtattg taggttgttt agctttagca
     7561 agagacgtta aagaaaaatc ttaatttgga aaaaagatcg tctcgactat ttgtttggtt
     7621 gatgcgatct ttttttcaat tagtaattga agggacgcct aaaagtgaaa aaaaaaatga
     7681 aagatgaagt attagagtat atcaaaaata atttaaagta ttacgatttt tctgctcaag
     7741 atattgcaat gaaattttgt attaaaagaa atgtcgcttc ccactatttg aatcagttat
     7801 tttcagatgg aaaattacta aagaatgatt ctgttagacc agtaatgttc aagtataatc
     7861 aacaaaagcc aaaagactgc ttttctaagt ttattggagc agacatatct ttgaaatcca
     7921 ctatagataa atgcaaagct actgtaatgt atccccctaa tggcctccca ttaataataa
     7981 aaggaaacag tggcgttgga aaaagttttt tagcaagttt gatctatcag tatgctcttg
     8041 atagaaaagt aattcataat gacgcaaaat ttgttgttgt gaattgtgca gattatgcaa
     8101 ataatccaga gctattgtca gcagttttat ttggatataa aaaaggagcc tttactgggg
     8161 ctgaaaaaga tactgcaggg atgctatctt ctgctaatgg aggatacctt tttttagatg
     8221 aggttcacaa tctaagtgca gaaaaccaag agaaattgtt tcttcttatg gattctcaaa
     8281 agtatcgtaa acttggtgaa agtgtgcaat gggagtatgc aaatgttcgc cttatcttag
     8341 ccacaacaga agataaaaat acctctttgt tagcaacttt tagaagacgc atcccagctg
     8401 aaatcactct gccagattac aataatagaa gtacatctga aaaaattcaa ttattatttg
     8461 aatttcttaa agatgaagca attacgattg attcaaaaat attttgttcg attgaacttt
     8521 ttactgatct tttatcgaaa acatttgaag gaaatgttgg agaattgagg aatgaaatca
     8581 agcttctttg tgcagaaggt tatttaggaa ataagagaaa gtcttcaatt tatttagggg
     8641 acaaactaga cagcggtttt tggatagatc cagaaattag tatagattta aaaaaaatgt
     8701 ttcttaaatc actttctgaa attgatttga ttgaactttt tcaaaatcac atttcaattg
     8761 aagaaagcat gagtcaatta agagatagaa tacttaaaga gtgtgcaggt tcttctgagt
     8821 ataattattt agaaagtgac gaatttatag cattaagaaa ctatgttgtt aataaaatat
     8881 caccaattat tgattcgaca ggtctatgtt tattagatga attcttggcg gatatcagtc
     8941 tatttataat gtttattgat gtaattgaag aaacatcaag aaattttttg ctgtctaagt
     9001 tcagaaaatt tcgtatgaaa aatgataaac aaaaattatt ggcaaatgag atttggagta
     9061 gtttagaagt cgaagaaagt aaacaagaac ttttgttgaa atggatactt tttcttgtaa
     9121 agaagttcta tgtgaaaata ccagaaaccc attgtctgat agttatgcac ggtaaaataa
     9181 cagcttctgc gatcgcaagg gaaacaaata agttgctgaa tacatatgtt tatgaggctt
     9241 tcgatatgcc aattgaaggt gagactagtg acttaattaa cctaatcaac aatttttgta
     9301 aaagtataga tacgacgaat ggactcattc tgctagttga tatgggatca ttggaacaaa
     9361 tgtatgaaaa aattgaatcc aatgtaatag gtgatttggt aattttaaac aatgtttcta
     9421 cagctttagc gattgaatgt gggattcaag tttgccaaaa aaagccaatt agtcattttt
     9481 accagatgga ttttgattcg tttcaagtta aagttcagta ttataaagga ttatcacaaa
     9541 agaaaaatgt aatagtatct tgtctctctg gtgaggggat ttctgaaaaa gttaaggata
     9601 ttttaaaacg gtatctcgat aatcaagtgg agattttgat ttttgatttt aacgcattaa
     9661 agaaaatagc aaaggaaaaa gatactatga tttttaagaa tacaatttgt gttctttcaa
     9721 ccactgaatt ttacattcaa ggaatcgatt gtttaaattt agaaaatcta attaatggga
     9781 atcaaacctt agaaaaatta aataagtata tggactcgga tagctgtgag ttctgcttaa
     9841 atgaattagt taaactcttt actattgaag gcgctagtac taagcttcgc tttttagatt
     9901 cc
//
LOCUS       DAYYGO010000100         9870 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000100 DAYYGO010000000
VERSION     DAYYGO010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.102"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(18..263)
                     /locus_tag="ACHA5T_002206"
     CDS             complement(18..263)
                     /locus_tag="ACHA5T_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFM8887759.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(378..956)
                     /locus_tag="ACHA5T_002207"
     CDS             complement(378..956)
                     /locus_tag="ACHA5T_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8887760.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(1033..1563)
                     /locus_tag="ACHA5T_002208"
     CDS             complement(1033..1563)
                     /locus_tag="ACHA5T_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFM8887761.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1567..2427)
                     /gene="rlmB"
                     /locus_tag="ACHA5T_002209"
     CDS             complement(1567..2427)
                     /gene="rlmB"
                     /locus_tag="ACHA5T_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFM8887762.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2420..2815)
                     /locus_tag="ACHA5T_002210"
     CDS             complement(2420..2815)
                     /locus_tag="ACHA5T_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFM8887763.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2812..4224)
                     /gene="cysS"
                     /locus_tag="ACHA5T_002211"
     CDS             complement(2812..4224)
                     /gene="cysS"
                     /locus_tag="ACHA5T_002211"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFM8887764.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(4221..4757)
                     /gene="epsC"
                     /locus_tag="ACHA5T_002212"
     CDS             complement(4221..4757)
                     /gene="epsC"
                     /locus_tag="ACHA5T_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFM8887765.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(4843..5082)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(5079..6539)
                     /gene="gltX"
                     /locus_tag="ACHA5T_002213"
     CDS             complement(5079..6539)
                     /gene="gltX"
                     /locus_tag="ACHA5T_002213"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFM8887766.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6709..7866)
                     /locus_tag="ACHA5T_002214"
     CDS             complement(6709..7866)
                     /locus_tag="ACHA5T_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFM8887767.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7900..9270)
                     /gene="radA"
                     /locus_tag="ACHA5T_002215"
     CDS             complement(7900..9270)
                     /gene="radA"
                     /locus_tag="ACHA5T_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFM8887768.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(9333..9860)
                     /locus_tag="ACHA5T_002216"
     CDS             complement(9333..9860)
                     /locus_tag="ACHA5T_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFM8887769.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT         2507 a         2213 c         1784 g         3366 t
ORIGIN      
        1 tcaggctcgt ttgcttttta aactgcttca gttaaaaatt cgatctcaac tgtccgagta
       61 agtacatctg aatagctgta agatactcgt tcaaaagaat tttcttcagg atccaaatca
      121 acaacgaata ctgaaggata ggtttctgtt aaaacgccgc ggcgttctgt ttgacgcttt
      181 cttcctgtct gagcaaccaa catgattggg ctgccgatac gaccttctaa atctttcttg
      241 attgaagcta aagttgttgg catacagttc acctctacta tcaactatac cacatatttt
      301 taaaattttc aagtttacca taaaaaaaaa agaaatgcaa taaaaataaa taagaagctc
      361 attaagattt ttctaaatta ttcctcatta taaaatttca agtgtttttt tattttgagt
      421 tttactctcc cgtaagcgct cattacttgc ttgtgatcca ttgctaaaca ttcagccatg
      481 tctacaaaat caacgccttg catataataa tgaaaaattt tagcttctat ttttgaaaac
      541 tggatttcgc aattttcgat tgcttctttt aataaaagat gctgaatcgc taaaggttct
      601 tgcaatgtga tctctgctaa aaagtctggt ccttctgtct ctattttttg gtcgattgag
      661 acttcatctt tttgatcacg acgcttcagc gcgttctgct tcctcaacag agaatagatt
      721 tgattgctta taatgctttt ataataactt ccaaagctaa cacctttgct tttttcgtaa
      781 gttctcaatg cctgatataa agcgatcctt gcttcttgtt cccaatcctc agaaacataa
      841 tttcttaaat aataggtgtt tttcaaacgt agtaaaagtg gaaaatattt ttgatacagg
      901 gcagcaaatg cttcttcgtc accattacgt gcagcgcgca ataactcctc agacataaaa
      961 taattcctcc ataaattcgt ttatggagga atcttatcat caatgattca tcgaactgat
     1021 tagaacgata tgttatttat tttttctcat taaatcgtcc aatttttggg acaatttttt
     1081 taactgctct tcattccatg gagaattacg tcggaaatca ataaaatgca tatctttatt
     1141 ttcttgggct attgtttttt ccgttttttt tatcgtctta tataattcat aagcagaagt
     1201 tcgtaatgct ccttgggaaa agatcatcca ctgttctgct aaatcgcttg tcgccactgt
     1261 cacttgtgtc aaacggtcat tcagctgacc agcgattctc tcgatataac tgtcagccgt
     1321 ttcgtcttct ttcgtaaaaa caacatccaa ctggtatttc ttataatttt gttggatgcc
     1381 cggaactagt tgggcatcga agacaacgat gacttccatt ccttcatatt tcgcataatt
     1441 tgataaacga tgtaaaagag cttctcttgc atccgccatt ttgtcctgct ttttaagctg
     1501 gacaagttcc ggccacgaac caatcatgtt gtagccgtct acaatcaaca gctgtttttt
     1561 catggtctac tccccctgac gttgggaaaa agcctgatac ataagcaaac cagcagcaac
     1621 actggcattt aagctctgga cgtgcccggt catcgggatc gtcagcaatt catccatttc
     1681 ttttttcaat cctggactca ttcctttgcc ttcattacca atgactaaag caattgatcc
     1741 tttggcattc catttttgga agtttgtgcc ggtcatatct gtgccaaata cccaaaatcc
     1801 tgtttctttg agctgttgca cagcttgtac caaattcgtc acgcgtgcaa cagggacatg
     1861 ttcgactgcc ccagtggaag tctttgttac gataggtgtg attccaactg ctcggtgttt
     1921 tggtatgatc acaccatcga cgccacttgc gtctgctgtc cgtaaaatcg acccaaaatt
     1981 gtgtggatct tcgatgctat ccaaaatcaa aaagaagggg tcttcttttt tctcttttgt
     2041 tcgttcaatc agttggtcaa gggataaata ttcataaggt gtaatcgcaa gaacgactcc
     2101 ttgatgcacg ccgtgatcgc tcaaagtttc taatttttgt ttaggaaccc atttgactgg
     2161 cacagcttgt tcacgtgcga tttctttcag ttcttctact ttttctcctc gtgaatcttc
     2221 ttgaagaaag agtttattcc ctcgcccttg ttgtagcgct tctactgcgg catggtgccc
     2281 aaagacgaaa ttatcttcta atgcttcttc attttgatca gaaactgact gatttcgtcc
     2341 agtttgcttt ttatttgaat atctattttg ttttttgtct tcaaatccac gtttcgattg
     2401 atgtttgttt gttctttctt tacccatttt tttcaccaac tttctgaata caccagcgga
     2461 tcaattcttc catccgctcg gtttgttttg tcaaatgcag atagcccatg agcgcttcga
     2521 accctgttgc gacccgatac gtcgtaacat cagcattttt tgcgctcgta tggcttttag
     2581 catttcttcc tctgcgataa taaagttctt cttcttctgt caaaagatgt tcttccagca
     2641 tttgctgaat caaaaaggct tgtgcattcg cagaaacata attcgttgcc gcacgatgca
     2701 gcttattggg ttttgtttct ccttgatcaa tcaggtggtc acgaacatag atttcgtaaa
     2761 tcgcatctcc aacataagcc aaggctaaac cgttcaattg tgtgtaatct ctcatgcttc
     2821 tcttctccat cgggttcctt gcggtgtatc ttctaataag atcccttttt ctttcaacaa
     2881 atcacgaatt tcgtcgcttc ggacaaaatc acgattgctt cttgcttgat ttcgttcttc
     2941 gatcaattgt tcaatctctt catctaataa ctcttctgct gtaaataaaa taccgaagat
     3001 cgaaagccat tgcgagaata aagcttctcc ttgtgccaag acagcggcag aaactttcgg
     3061 ctggtctaga taagtgttca gccacttagc aagctcatag accactgtga tcccatttgc
     3121 tgcattaaaa tcatcgtcca tttcttcgat gaatcgctgt tctaaatttt ctaacaaagc
     3181 gattttatcc gcatcatctg ccaaattctc tgaggcagtt tcttggcgga atttcaaatt
     3241 gtcaaacgta ttcttcagtc gttgtaaatt ggtggctgct tctttcatcg tcgtttcact
     3301 gtagcggatc ggacgacggt attgtgtggt cgccatgaag aaacgcaaga cttgtggatc
     3361 aacgtttttg atcatttcat gtacagtgat gaaattccct agagatttac tcattttttc
     3421 atcgtcttcg ccaatcgtta catagccatt gtgcatccag taattagcga attttttccc
     3481 tgttttcgct tcactttgtg cgatttcatt ttcatgatga ggaaattcta aatcttgccc
     3541 gccaccatgg atatcgatcg tttctcctaa atgttttgtt gccataacgg aacattcaat
     3601 atgccaaccg ggacgtcctt tgccccatgg tgaatcccaa gaaatctcgc caggttttgc
     3661 ttgtttccat aaagcgaaat ctaatgggtc ttctttttgc tgctgttctt cccctgtacg
     3721 ctgacttgct ccgacttcca gttcatcgat ggattggtca cttaatttcc cataattttc
     3781 aaatttacgg gtacggtaat acacatcacc tgaagattca taagcatagc ctttttcaac
     3841 caacacttga ataaatgcta aaatatctgg catatgatcc atgactcttg gatgcagtgt
     3901 agcaggctcg acattcaacg ctttcgtatc ttcttcaaat gctcggatga aacgatcagc
     3961 aacttctggt gctgtaatac ctaattcgtt tgctgtacgg atgattttat cgtctacatc
     4021 cgtgaaattc gatacgaaat cgactttata gcctcgatat tcaaaatagc gtcgaattgt
     4081 atcgaaagca atggtgctac gagcatttcc gatatggata taattataca cagtcggtcc
     4141 gcagacatac ataccgacat gtcccttttg tatagggatg aattcttctt tttttctggt
     4201 caatgtattg tagattttta tcattctgat ctcctcactt tttctccttt gattcgaacg
     4261 acttttgctg gtactcctac cgctgtagcg tcgtctggaa catctttcaa cacaacggca
     4321 cctgcaccga ttttggcgtt ttttccgatc gttaccggtc ctaatatctg cgcattcgca
     4381 gagagcatgg ctccttgttt aacagttggg tgtcttttac ccgtttcttt tcctgtgccg
     4441 cctaatgtta cgccatggaa taaaatcaca tcgtcctcga tttctgcggt ttcgccaatg
     4501 acgattccca ttccatggtc gatgaacacg ccagttccaa tcgttgctcc tggatggatc
     4561 tcaacaccag ttaggaaacg ccagaattga gcattgatct tcgccaataa aaagagccgg
     4621 tgctgatata aaaaatgaga gaaacgatgc caaaacaagg catgaaaacc agggtatgtc
     4681 aaaacgatct cagctgtcga acgtgcagcc ggatcgtttc tctttgctgc atcaatcgct
     4741 cgggtaaacc aactcatgac tttccactcc tttcgtaaga attttatgta ggaaatcatc
     4801 ttcttataaa gaaaagaagc gccttttaaa taagtttcct tatcaaaaga cgctttccgc
     4861 gtggttccac ttttcttcat aatcaatgat tatctctgac agctaacgcc tgttatacgt
     4921 tcttggctac tttgtttcct ataaaacagt ttttcctgtt ttctgtgttt cgccaagacc
     4981 actcccagag gcatttcggc aaccattcat tgataccttg caccacccgg tatctctctt
     5041 gtaatgatca gctgcttact tttttctgtt catcgtgttt agttcagtac ttgatttaaa
     5101 tgtgctaatg atttttcacg accaagcaat tcgatcgttt caggcaattc tggaccatgc
     5161 atttgtcctg atacagctac acggattggc atgaaaagat tttttccttt tacgccagtt
     5221 tctttttgaa cagctttgat ggcagctttg acagttggag catctactac ttccatttgt
     5281 tctagctgtg ctttgaatgc gtggagaaca gtaggaactg tttcaccagc aagtacttct
     5341 tttgctgctt cattcaatac aggatgttca ttgaaaaata gcgtagacag atcaacgatt
     5401 tctgcagcat agctcatttg aggttgatac aggctaacga tcttcttcac ccattcgatt
     5461 gtcttttcat ctgggttttc tgctacacgt ccatcagcga ttagataagg cagacacatg
     5521 tcagtaaatg catctagatc cattgctttg atgtaatggt tgtttaccca ttgcagtttt
     5581 ttcgcatcga aagctgcggg tgatttactt aggcgttttg gatcgaacat tttgatcaat
     5641 tcttggtgtt cgaagatttc gtcttcgccc acaggtgacc agcccaataa tgcaataaag
     5701 ttgaacattg catctggtag atagcctaat tcgcgatatt gttcgataaa ttgcaagatt
     5761 gattcgtcac gtttgcttag ttttttacct gtttcactgt tgatgatcaa tgtcatatgt
     5821 ccgaaggttg gtggtgtcca gccaaatgct tcatagatca tcagttgttt tggtgtattg
     5881 gcaatatggt catctccacg taaaacgtga gtgattttca tcaaatgatc atcgaccgca
     5941 acagcaaaat tataagttgg cataccatcg cgtttttgta tgacaaagtc gccaccgata
     6001 ttatcggatt cgaacgaaat agcgcctttc accatatctg taaatgcgta ttctgtattg
     6061 cgaggcacac ggaaacgtac aacagattct agaccttgtg cttctttttc tgcttgttct
     6121 tcaggtgtca agttcgcaca tgttcctgcg taatgaggca tttctcctct tgcgcgttgt
     6181 gcttcccgtt ctgcttctaa ttcttcttct gtgcaatagc atttgtatgc tcgattgcta
     6241 gcaagcagct gatcaatcag cggttgatag atttctttac gttcagattg acggtaaggg
     6301 ccgtattctc caggattttc aggagattca tcccaattca ttcccaacca agcaaggttt
     6361 tccaactggc ttttttcgcc atcttcgata ttacgttttt ggtcagtatc ctcaatacga
     6421 ataataaagt ctccatcatt gtggcgggca aacaaataat tgaataatgc agttcttgca
     6481 tttccaatat gcaggtgccc agttggactt ggtgcatagc gtacgcgtac tttcgtcatt
     6541 ttcttcctct tctttcaaac tagactttga ctaacagcaa aaattccgtc agttacggct
     6601 tctcaaatat catttattta aagccactca gcacaaattt taccttttct aaggtaaata
     6661 gtaaagccaa ctggttgaat ttccttacca gttggctgaa tttctttttt attttgattg
     6721 tttagtcttt ttctctgtct cttttttatt gtcgattcct cgtcctgcat gtgcaggttt
     6781 agcaaagatc atgcgtccag cagctgtttg aagggcactt gttacaacga cttcgatacg
     6841 ttcattcatg taatgctgtc catcttctac gacaaccatc gtgccgtcat caaggtaggc
     6901 tactccttgt tggcgttctg tcccggcttt tactactaga acattcatcg tttcaccagg
     6961 gatgacgact ggttttacgg catttgccaa cgcattgata ttaagaacag gtacattttg
     7021 gaattcagag actttattca agttatagtc attggttaca atgacaccat ctaaaagctt
     7081 agccaatttg atcaatttgc tgtctacttc actgatatct tcaaagtctc catcgtacat
     7141 ttcgacagat ataccctctt ctttttgcaa ggcatttagg atatctaatc cgcgacgacc
     7201 acgcacacgt ttcaaactat cccctgagtc cgcgatgtat tgtagttcgt aaagaacaaa
     7261 attagggatc atcaaggttc cttctaagaa tccagtcttt gtaatatcat aaatccgacc
     7321 gtcaatgatc acgcttgtat ccaagatttt atatttatgg aaattttctc ctgattttcg
     7381 ttctaacagt tgactctcaa cttgactagc tgtttcttgc tcgatttttt tgcttcgtga
     7441 accaaaaact ttcttccatt cttcgattcg tgtcgttccc attctcaatc ccagatagcc
     7501 taagatgatc atcagtaaaa tcggcaatac actattgacg aatggaattt ccatattgta
     7561 aagaggggtc gagatcacaa cacctaaagt caatccgatg atcgcaccga tcgcaccgaa
     7621 gagaagataa gtcaagctga cctcacttag tttttgttct acttgtttga taatagctga
     7681 gatatgtttg gccaacagca aagataataa aaagaaaata agtgcgccta acaggctgtt
     7741 ggtaaatgta ttattcagcc atccgtttga ctgttgaccg attgcctgcc aagctgaggg
     7801 taaaaatgaa atacccaagc ttgcgccgac aatgatcatc agcaaagtaa tgacacgttt
     7861 ttgcatcgtt tttcctccgt ttcgacaatc atttattagt tagcggaaca cacgcttcaa
     7921 cgtttcagcc aaagttgcta ctccaactat ctcgattcct tttggcgctt cccagttacc
     7981 taagttgttt ttaggtaggt atacttttgt gaagccgagt ttttggactt ctttgactcg
     8041 ttgttcaata tgactgacac gtctgatttc tccagtcagt ccaatttcac cgatgaaaca
     8101 ttccgttggc tgtgttcctt tttctttata gcttgaagca atactgacag cgatagctaa
     8161 atcaatcgct ggttcattta attttacccc acctgcagct tttaagtaag catcttgatt
     8221 ctgaagaagc aacccagctc ttttttccag cactgccatg atcagagata cccgattgaa
     8281 atcaagacct gttgttgttc gtttggcatt tccgaacata gtaggtgtga ctaatgcttg
     8341 gatctctacc aaaattggac gtgtcccttc cattgccaca acgatagcag agccagttgc
     8401 accgtctagg cgttcttcta agaaaatctg tgaaggatta gcgacttctt ccaatccatg
     8461 ctcatgcatt tcaaaaatcc caatctcatt cgttgagcca aatcggtttt ttactgctcg
     8521 caaaatccga aaactatgat gttgttcacc ttcaaaatat aacaccgtat ccaccatatg
     8581 ttctaacatt cttgggccgg cgatcgagcc ttctttggta acatgaccga cgataaaaat
     8641 ggctatccca tttgttttag cgattttgag tagttctgca gtggtttctc ttacttggct
     8701 gacacttcct gccacacttg tgatgtctgg ctgcgtcatc gtttggatgg aatcaatgat
     8761 aacataatca ggggagatat gttcgatggc acgactgatt tcgttcatat ctgtttcagc
     8821 gtatagataa aattctgtgt tgatacttcc gaggcgttcc gcgcgtaatt ttatctgctc
     8881 tgcgctttct tcgccggata catataagac ttttccgcct atggctgcta attgctgaga
     8941 aacttgtaac agcaaagtgg atttcccgat acctggatcg ccaccaatca agaccataga
     9001 ccctggcacc acacctccgc ctaacacacg gttcagttct tctaattttg ttttgatccg
     9061 aggttctttt ttagggacga cgtctgcgat ttttgttggt tttgcttttt ctcctgtcaa
     9121 acttgtccga gttctgcggt ccgttgtatc ttgttcgatt tcttcgacca tcgtattcca
     9181 ttttccgcaa tttgggcagc gtcccaagaa cttaggcgac acatacccgc aattttggca
     9241 aacaaattgt acttttgatt tttttgccat tgtaccgcct ccttttatcc taatcatgag
     9301 acattaaatc aagcttaagt atctttaacg ttttatttac tggaagaacc aaatccgcct
     9361 tgacgttgag cagtcgtttg atcttgatct gcttttaaaa acggcaaaaa gatcccttgg
     9421 ccgatacgct cgcctttttt gatcactaca tccttgaaac caaagttggt gaattggaac
     9481 atgatatggc cttcatttgc ctcattattg taataatcac tgtcaatcac acctactccg
     9541 tttgtcaaca ctaaaaaccg cttcaacgga ttgcttgaac ggttagcaag ctgcaaaaat
     9601 tcatcttctc ccatgtaagc tttgattcca gtaggcacga gggttggttt caattgtttt
     9661 tcattttctg atacatcttt tttcaatcca tctgcgactg tttccaaccc ctttttccag
     9721 cctgctttcc agatacttgg aatcacgacc tcttctgctg cttcaaaatc atagccagca
     9781 gcatgatgtg tagcgcgctg cggtagattg atattttgat ctttatattt agaaataact
     9841 tcaaatcctc tttttctcac cggttttcct
//
LOCUS       DAYYGO010000101         9798 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000101 DAYYGO010000000
VERSION     DAYYGO010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.103"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(185..1033)
                     /locus_tag="ACHA5T_002217"
     CDS             complement(185..1033)
                     /locus_tag="ACHA5T_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8887770.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1197..2099
                     /gene="gnd"
                     /locus_tag="ACHA5T_002218"
     CDS             1197..2099
                     /gene="gnd"
                     /locus_tag="ACHA5T_002218"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFM8887771.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            2101..3102
                     /locus_tag="ACHA5T_002219"
     CDS             2101..3102
                     /locus_tag="ACHA5T_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFM8887772.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFNVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMCEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3099..3383
                     /locus_tag="ACHA5T_002220"
     CDS             3099..3383
                     /locus_tag="ACHA5T_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFM8887773.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3416..4213
                     /locus_tag="ACHA5T_002221"
     CDS             3416..4213
                     /locus_tag="ACHA5T_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8887774.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLSSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4227..5060
                     /locus_tag="ACHA5T_002222"
     CDS             4227..5060
                     /locus_tag="ACHA5T_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8887775.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5078..5935
                     /locus_tag="ACHA5T_002223"
     CDS             5078..5935
                     /locus_tag="ACHA5T_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8887776.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            5957..6466
                     /locus_tag="ACHA5T_002224"
     CDS             5957..6466
                     /locus_tag="ACHA5T_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFM8887777.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6503..6904
                     /locus_tag="ACHA5T_002225"
     CDS             6503..6904
                     /locus_tag="ACHA5T_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887778.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7016..7369
                     /locus_tag="ACHA5T_002226"
     CDS             7016..7369
                     /locus_tag="ACHA5T_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887779.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7403..7846
                     /locus_tag="ACHA5T_002227"
     CDS             7403..7846
                     /locus_tag="ACHA5T_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8887780.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7863..8948
                     /gene="uxuA"
                     /locus_tag="ACHA5T_002228"
     CDS             7863..8948
                     /gene="uxuA"
                     /locus_tag="ACHA5T_002228"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM8887781.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            8941..9654
                     /locus_tag="ACHA5T_002229"
     CDS             8941..9654
                     /locus_tag="ACHA5T_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8887782.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
BASE COUNT         3256 a         1552 c         2165 g         2825 t
ORIGIN      
        1 atcgcatcta acaagtaact cgaataatac caatacaacc ttctccaaag atgcgatgac
       61 atacgttcta acccaccgac tattgatgtt ctgtaccacc cttgcagaac caaaaaagcc
      121 actaccaaat atttggtagt ggcttttttt gttttatgga tttttcgctt tttctgttat
      181 ggtgctattc tttgatttca gaaatagtat gtcgaacttt agcgatagaa cgacgtgatt
      241 ctttttcgtt gtaaaacatc aaaatcacat atagagagtc caaaatgctt agttgagaaa
      301 tacgggaagc taaagcttca gatcggaatt ctgtttcttc agaaatagaa ataaaaacga
      361 catcgccaag tttagcaagg gaagaattag cttggctagt aataacgatt gtttttgctc
      421 cagtttttct tacggtttca gcaatatgga ttgtttcttt tgattgccct gtgtgagaaa
      481 tcaaaacagc acagtctttt tccgtcaata aagaagcttc catcaattga atatggtaat
      541 ccgtgctgtg agaagttgcg ataggggaac gcaaaaattt atgataagcg tcagttgcta
      601 caatctctga accgccaaca ccgaaaaaat agacacgttt agattcattg ataatagtag
      661 ccgctttttt tagatccttt tgttgtaaca acttttttgt gtcatttaaa gttgaaatac
      721 tagactcgaa caccttttgc gccatagtga gctcagtatc tgttttctga atattttcgt
      781 ggatagaaat agcagataga tcattttctt ggatcaacat ggccatttta aaatctttaa
      841 atccattata accaagtttt tttgtaaatt gaaaaaaagt agagtcagca atgcccagtt
      901 cacttgctaa attgctaata ggactatgac ttactttgct tggattttct aaaatataat
      961 cggcgatagc ctgttctttt gggctgaagt cgttgtacat tgttttgatt tgtagtctta
     1021 tggagacgct catgagaatt cctcattttc ataataagat tatcattttt tccgttctca
     1081 ttaatgttaa agatacaagg agagtaggaa aaaatcaaac aaaaaatatt tttgtaaaat
     1141 ctatttacaa aaaaattttt ttgtataatg agggtataag aaatggaggc gctatcatgg
     1201 aaattggtgt aattggtttg ggaaaaatgg gattgaattt agctttaaac ttaaaagagc
     1261 accatcacaa agtacaagga ctcgacattt caagtacagc tgttagtcag gcaagagaaa
     1321 atgacattcc tgcttatcaa gagatctctg aatttctagc tacttttgag cagaaaaaaa
     1381 tcatctggtt aatgcttcct gctggagaga taaccgaaaa catgttaaga aaactttttc
     1441 caaaactcgg accaggagac attattattg aaggcggaaa ctcttattat aaagattcaa
     1501 tccgtcgagc agaagagttt cagaaaaatg aaatcggtta cttggattgt gggacttctg
     1561 gaggaatcga aggagcacga catcaagcct gcttaatgat tggaggagat aaagaaactt
     1621 ttctatccgt ggaacaatta ttcaaagatg tagcaattga aagaggatat ctgtatgctg
     1681 gtgcatccgg aagcggacac ttcttgaaaa tgatccataa tggaatcgaa tatggaatga
     1741 tgcaagcaat cggtgaagga ttccaacttg tacaaaaaag tgagtatgct tttgacttgg
     1801 aacaagtcgc acgtgtttgg aatcatggtt cggtgattcg cggctggctg atggaaatca
     1861 tggaagatca gttaaacgaa catcccaatt taagtaatta tcggggaatc gtttctgcat
     1921 caggtgaagc aaaatggacg gttgaaacag cgctagaaat ggctgttcca gttccagtaa
     1981 ttgcaactag tttgttcatg aggaatcttt cacaggaaga agatagtttt tcagcaaaag
     2041 tagtttcagc actaagaaat gggtttggcg gacatgaaat agttgaaaag ggtgagtaaa
     2101 atgccagatt atcatatagc aatcgttaat tccagcagtt tcggaagagt ctttccacac
     2161 cacttggaaa gattaaagtc agttggagac gttcatttct ttaatgttga tcaagagatt
     2221 ccaggaaaag aactagcgga gttacttaaa ggattcaata tcattattgc aagtgttacg
     2281 ccattttttc gacgagattt ttttgaaaac aaagatgaac tgctattgat ttcccgacat
     2341 ggtattggtt acaacaatat tgatttaaaa gcagcaaaag aacaccagac aatcgtatct
     2401 atcatacctg ctttagttga acgaaatgct gtagcagaaa ataatataac taatttgttg
     2461 tccatgcttc gacgcacgac acaagcacaa gcaagagtca aggacgatcg atgggaagat
     2521 cgggcggagt tcgtcggtag gacgctgttt aacaaaacag ttggtgtgat tggtgcagga
     2581 aataccggaa gcggagtaat cgaaattttg cgtaacggtt ttcgctgtga tgttctagct
     2641 tatgatcctt acaaatcaga tttatacatc gaaagctttg gtgcaaaaaa agtagaactt
     2701 caagaattac tggaacaatc agatatcatt tgtttatgtg cgaatttgac agaagagaac
     2761 tatcacatga tttctgataa agaagtcgat cagatgaaag aaaatgttta tctatctaat
     2821 agtgcaagag gcgctctagt tgatgaagaa gcgatgatta atggtctgaa aagcggaaag
     2881 atcgcaggat atgccacaga tgttttagaa gaagaaccgg gacgtcaaga tcatccgtat
     2941 ttagcgtttg aacaagtagt gattacgcca catatatcgg cctacacaag ggaatgttta
     3001 gaagcaatgt gtgaaaaatg tgtcagagat gtcgaacaga tcagtcagca ccttcttcct
     3061 gaacgttctg tccagacaaa gagcaactat ataaaaggat gatgaagaaa atggtgaacg
     3121 gtttaaaagt gaaaacaggc cctcaatttt atttatatga agaaggtggt atttcaaaag
     3181 tttctgatct cttgaaatca tacggcgcga aaagagtact ggtcacccac ggaacggtat
     3241 catgggaaaa ggcgctacct aaattagtat ttttgaatga cgaaacgatt caattttttt
     3301 atcatcgtta ttctggagaa tgtagttatg ctgaagccag acgaatagct acaatcatca
     3361 aaaaaatgaa atcgatttcg tagttggcgt tggcggagga aaactgaccg atctagtggg
     3421 ttatagtgcc aatcttgcaa gaatcgattt cggagtcatc cctactttag ctagtaattg
     3481 tgctccatgg acacctcttg ctgtcatgta taaggagaat ggacaagcag aagggaaaac
     3541 agaacacttt tacagacaag ctgctttttt tataacagat ccccaattag tgattgattc
     3601 ccctgttcca tatttcattg ccgggttagc agatacacta gcaaaatggt atgaatctga
     3661 gctcatctta gaacaagatt ttttgcaaga tgaatcattt ttgaagctag cacaagatac
     3721 tgcgaagatc tgcaaagaag agattttgaa tcattctgct aaagcaatcc aagatatggc
     3781 acaaagaaaa ctgtcatcgg agttcaaaca gctttcagag attatctttg ctgttgctgg
     3841 tttagttgga ggattaggag ataaatacgc aagaaatgtg gcagcacatg ctatgcatga
     3901 cgctatcagt aaatatattc cggaaagcca tcgttttcta catggagaaa aagtagcgta
     3961 tgggattttc tatcaactgg cactagaagg tcgctgggaa aaaatagacg agttatatcc
     4021 tttgtatcgg gaattagaac tgccggtttc tctagaacaa atgggattat ttccgaaaga
     4081 taaacagata atagataaaa tggctgaatt tattgattcg aaagagaaag tgcatttgat
     4141 tcctatagag gtaacacaga aatcactgat acagtcaatg atggaactgg aaggttatat
     4201 cagaaagatc tgaaggagga ggacacatgg aaacattaag tgtagtacaa agtttgttga
     4261 ttgctttatg ggtagccgcg gttatgtccc gctggttagg tggaggagcc acgttgacct
     4321 tacgtttttc tcctttgatg actggattag tcgtcgggtt gatcatgggg gaagttgcac
     4381 aagcgatgat tgttacagcc gctttgcaaa tgatttatat gggggttttt tctccaggag
     4441 gatcaatgcc agctgaacca tctattgcag cagctatcgc agtaccagtc gcattattag
     4501 gggatctgtc gccagaagca gcgattgccg ttgcggtgcc agtcggattg ctcggaagtt
     4561 acttgtatca gtttcgtttt ttcatcaata cttttttagg taaatataca gacaaagctg
     4621 tagaagattt gaatacaaaa gctatggccc gatcaattat tctttatcca acggttgcct
     4681 catttttatt attcgttccg cttgttttca ttgctttata tttaggggcg ccattgatcg
     4741 ctgatgtaat caaagcttta gagggaacag tcgttatcca cattcttgaa gtagtaggtg
     4801 gagggttagc ggcaatcggt atcgcgacaa ctgtatacgt tatcggtcga aaagattatc
     4861 tggtcttttt cttccttgct tattttatga gtgttgtttt gaaatcttta gatattacga
     4921 tggttactta tgccattttt gggattatta tcgctttgat ctttgtccag tcgcaaaaag
     4981 ggaaacaagt acagcctgcc acaagcaata acaattcagc atcttttgat gacgaagatg
     5041 actatgatga cggattttaa tttaggagga atgggaaatg gctgaaataa tggataatca
     5101 aacaggtgct cctgaagaaa tcacgaaaaa agatgtaaca aaagcctatc tgagatggca
     5161 ttttgcaaac gaaattcctc actcttttga acgttacttg gcgccatcac tactatacgg
     5221 aatgatgcca atcttgaaaa aactttataa agatgaagac tctttaaaag cagcatataa
     5281 acggcagtta ctgttcttca acactcaatt aagctggggt ggcggtgtga ttactggcct
     5341 aatgtcttct atggaacagc aaagagcaga agaagagtat gaagaaaaag agatcttgat
     5401 gcaggatgac ttgatgtata acacgaaagc gggattgatg ggggcactag caggaatcgg
     5461 agatgcaatc gattcaggaa ccgtccaata tatttttatc gcaatcgcgg taccttgggc
     5521 acaacaggga agtcctttag gcgcactttt tccttttatc tgctttgggt tgtatcaggt
     5581 tttactaggg gtatttttcg caagacaagc tttcactatg ggccgtaatg ctacaggcct
     5641 aaagcaaaac actggtgttc agacagcgat tgaaatgcta tcagttttag ggttgtttat
     5701 gatgggggtg cttgcgggaa actatgtaaa agtagaatct tcattgaaat ttgcgatatc
     5761 cggacgtgag tttgttatcc aggaaattct tgatcaaatc gtgccaggga tattaccttt
     5821 agctgtcgta atgggtgtgt acttgttcta tacgaaaaaa ggattgaaag tcacacaagc
     5881 attattatgg ctgactggaa tattaattgt gctagcagga atcggtattt tataaaaaga
     5941 agggaacgtg aataagatgg gtgtagtcaa tttagcgcgt gtggatgagc gtttgatcca
     6001 tgggcaagta atggtaacgt tatctcaaaa aagtggagtg aattccattt ttgttgtaga
     6061 cgaagttgta gcaaaagata aatttatgag agatctttac aaaaatgcag gaagccgtac
     6121 aggacaaaaa acaattgtca ttacaccaga gaaagctaaa ttctattggg atgaatatca
     6181 atttaaagaa tacaattgta ttttgatcgc aaaaacagtc caaacgattt acgacttagt
     6241 aaaacacgga atccccatgg aagaattgaa tattggaggg attgctcaaa aaaatcctga
     6301 tacagatctt ttagtgacga aatctgttta tttaaataaa tcagatgctg aaaaattgaa
     6361 agaaatgaat gaacaatatg gcgtaaagaa tatttatttt caagccactc catctgcacc
     6421 taaaacagga ttaaaagatg tattgaacaa gtttgatttg tcataaacaa atgcttagaa
     6481 aaaagataaa gagggcgaaa aaatgaagaa gaacgagctg tttcgtgatt gggaatttcg
     6541 ttatcgctat atttaccgta agcgccgtac aaaaaagagt aaacagcgtt ttctttcagc
     6601 attagtatca gatatttaca gtatgagaac agatgtgaca gtcattgctt acgatacgct
     6661 agcctaccga tcaaaaaata tttatgttgg agatatcgaa aaagcagaga aagttatctg
     6721 tacttattac gatacacccg ttcatgcatt aggaagttat ttcatgtttg attggaaaga
     6781 ccaaaggaaa aaaacaatct actctatatt attgtccttc attttgttgt tcagtttagg
     6841 ttggtgggga atgatgatat ataacaaaaa tcctcatcac gtattcgatc tgctatccgt
     6901 ataaacaggt atcactgtac tagcatttgg gagctacttt ttcttattgg gaaaagcagc
     6961 tcgcggatgg cctagcagac aaacttttat tcgaaatact tcatccatat taacgatgct
     7021 agaaatgatc cgtacgattg atgatccgag tgtagcgtat gcgtttgttg atgaaggctg
     7081 ttatggagaa aagggcttag acagcgtgag atcgggtatg aaaaaagagg ctattctttt
     7141 ttatctagac tctgttgggg cagatacacc gctacagttt tcgggaaatt atttttcaaa
     7201 taaagaacag tggctgaaac aagttgataa gttgaaagaa aaaaacgtca attatatttt
     7261 tagtgcaaga aaaaagcaag cgcagttctt ctatttgacc aaaacagatt tacgagggaa
     7321 aacatttaat tggcaaaatg ccaatcagat tattgctctt tttcgataag taaaaagtgc
     7381 aaaacaagga gtgagaagac taatgttgga aatcatgatc gttacacatg gtaagttgag
     7441 cgaaggactg aaagatgcgg cggaagtaat tgtcgggaca tcgagccata ttgaaacaat
     7501 gtgcttagag tctggggagg acatccaaac attcggagag aaggtcaaag aaaaattaac
     7561 tatgatcaac acagcagaac ccatgctagt atttgttgat ctaattagcg caagtccgta
     7621 caatcaagcg ctcttggcag tcaatgaact gactgaagaa aagaaagcag ccacttatgt
     7681 catagggggc gtgaatctgc caatgttgtt agaagcaatc aatcatcaga tgcttcacac
     7741 accgatagaa gaagcggtgg aacaaattgt caaacaagga aaagataatt taactgtttg
     7801 gcatgcttca gcggaagaaa aaaaattaga tgaggacgat ttttagaagg aaggctgaaa
     7861 agatgaagtg gggatttaga tggtatggtg aaaaagggga ttcgatccca ttaaaatatg
     7921 ttcgccaaat accgggaata aatgggataa ttggaacttt gctgactaaa atgcctggag
     7981 atgtttggga gatttcagaa attgaggagt tgaaagcatc tgttgagagc caaggactgg
     8041 aactgcttgg aattgaaagt gtagcgatcc acgatgcaat caaggcaggg acagatgaac
     8101 gagattacta tattgataac tatatacgaa caatcaaaaa cttgtctcga tgtggggttc
     8161 gattgatttg ttatagcttc aaaccaattt ttggatgggc gaaaactgat ttgtttaatg
     8221 aaaacaacga tggaagctat tctcttttat acgatcagaa aatcgtcgat cagatggagc
     8281 caggagaaat gtatcaattg atacacagcc agtcaaaggg attcaaattg ccgggttggg
     8341 aagaagaacg gttgaaaaaa ttcgctcagc tggaaaaact gtatgaagga atgaccgaag
     8401 aagaattatt tgaaaatctg acttattttt tagaaaaagt gattccagtg tgcgaaaaag
     8461 cagatgtaaa aatggctatc catccagatg atccgccatg ggaacttttt ggttttccta
     8521 gagtgacgaa aaatcttggc gacttgaaga aaattctaaa tgcagtagat tctccatata
     8581 atggactaac gctgtgcaca ggatctttag gggcaaatcc ggaaaacgat ttagttgaaa
     8641 tcatccatga ggtaggttcg cgcatccatt ttgtccactt cagaaacgtg gagtttttag
     8701 gagaaagaaa gttcaaagaa acggcacatc cctctacaga aggctccctt gatatgtatc
     8761 atattatgaa agcgttggtg caagaagggt tcgacggtcc tatacgccct gatcatggta
     8821 gaaccgtgtg gggtgaagtg gctatgcctg gatatggcct gtatgatcgt gcaatgggac
     8881 tcacttacat gcaaggtcta tatgaggcga taagtaagga aaaaccactg ggagagcatt
     8941 atgaataaaa tcaagctctt catctttgat atggatggcc ttttattaga aactggacgt
     9001 ttagcttatc gtgcttatgt aaaatcagca caaaagtatg attatgaaat gcgtaaggaa
     9061 gtctattatt tactaactgg ccaaacagaa atggcgataa gagaacaaat gggcattttg
     9121 tacggagaag atgttccata tatcaaatgg cgagaagcaa tcaatcaata taaagaaaaa
     9181 atcgtgaaag aagataagag agtatatacg aaaaaaggag cagaagaaat tttatctttt
     9241 gcaaaagaaa gaggaatcca tacaatcgtg gcttcgtcta atacacgggt aaaagtagaa
     9301 atgtatttaa aaatggaaaa tctatatgcc ttgtttgatc aaatcatttc aggggatgat
     9361 gtaaaaaaag gaaagccgga accagagatc tttttgaaag cttgttcaaa aatgaatatt
     9421 cctccaagtg aagcacttgt atttgaagac tcgattgcag ggatcgaagc agcaaggcga
     9481 gcgggcattt tgtcttttct catcaaagat catttgatag ggttgcccga tcataatggg
     9541 aagcataagt tgaaagtaga tgtaagttta tatgaatcca ataaagcaga ctatgaattt
     9601 tatgacctgc atcaagcaaa acgattttta gaaagtaaag ggttgtatct ctaataaaga
     9661 ggactatttt ctagtcctct ttatttattg tcctgcacga ctgagggaac agatgaaaaa
     9721 gaagtaattt tttattttga agtatctacc ttatttgtag tgaacaattt agaacaaaca
     9781 caaaacgcat ctactgtt
//
LOCUS       DAYYGO010000102         9722 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000102 DAYYGO010000000
VERSION     DAYYGO010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9722
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.104"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            197..3004
                     /locus_tag="ACHA5T_002230"
     CDS             197..3004
                     /locus_tag="ACHA5T_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8887783.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            3195..3686
                     /locus_tag="ACHA5T_002231"
     CDS             3195..3686
                     /locus_tag="ACHA5T_002231"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFM8887784.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            3789..4778
                     /locus_tag="ACHA5T_002232"
     CDS             3789..4778
                     /locus_tag="ACHA5T_002232"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFM8887785.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            4811..5614
                     /locus_tag="ACHA5T_002233"
     CDS             4811..5614
                     /locus_tag="ACHA5T_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFM8887786.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            5635..6549
                     /locus_tag="ACHA5T_002234"
     CDS             5635..6549
                     /locus_tag="ACHA5T_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8887787.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            6632..7006
                     /locus_tag="ACHA5T_002235"
     CDS             6632..7006
                     /locus_tag="ACHA5T_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFM8887788.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            7145..7390
                     /locus_tag="ACHA5T_002236"
     CDS             7145..7390
                     /locus_tag="ACHA5T_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8887789.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            7562..8626
                     /locus_tag="ACHA5T_002237"
     CDS             7562..8626
                     /locus_tag="ACHA5T_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFM8887790.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            8872..9297
                     /locus_tag="ACHA5T_002238"
     CDS             8872..9297
                     /locus_tag="ACHA5T_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8887791.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            9290..>9722
                     /gene="nusB"
                     /locus_tag="ACHA5T_002239"
     CDS             9290..>9722
                     /gene="nusB"
                     /locus_tag="ACHA5T_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFM8887792.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKE"
BASE COUNT         3177 a         1603 c         2157 g         2785 t
ORIGIN      
        1 ttataaaaat cgtatcggga tttttcgttc ttttaaattg aacatttcta tctatccgcg
       61 tgttatcaac aaaacaaaat caggtaaata gttgacgaaa cagaaataaa tacatagtat
      121 tatgttttga agcctaagta caatagtcta tagaaaagga gcgataccat tggcaaaacg
      181 cattgaccga atttatatgt atgtcaaaga aaaaaccgct catttaacaa aggcagaata
      241 tgatcaagga gtaacaacac aggaaattgc tgaagtgtta gggattcagc gaacaaatag
      301 cagtaaagat ctgaatcagc tagtgcgcga aggaaaactg ataaagacag acggacgacc
      361 agtacgttac atatatcaaa cactcgttac gcacgtaaag ccagttacaa aacatgtagt
      421 cagttacaaa gaaacgccgg tctctgaaga aaaaaataca ctccaaatag atactaagga
      481 tatctttgca aaaatcattg gagctaacgg cagcatgaaa aattcagtgg aacaggcaaa
      541 agctgcgatt ttgtatccgc caagaggatt aaattgtttg attacaggac ctaccggatc
      601 aggaaagact tattttgcac acgcgatgta tcattttgca aaagcgcatc atgtaataga
      661 tgaggaaacg gaactgattg ttttcaattg tgctgattat gccaataacc ctgagctttt
      721 gatgagtcat ttatttgggt acgcaaaagg tgcttttaca ggcgcagatg aagaaaaaat
      781 ggggattatc gatcaagcgg atggcggtat gctgtttttg gatgagattc accggttgcc
      841 gcctgaaggt caagaaatga tcttttactt tatggatcat ggaacttaca gtcgtcttgg
      901 ggaaacaacg aaatcccatg aagcaaatgt acggatcgta ggtgcgacca cagaagaccc
      961 aggttcttct ctattagaaa cttttgttcg gcgtatccca atcaatatca aacttccggc
     1021 ttttgaaaaa cgtcctgcaa atgagaaagt cgatttagta aaaatcatga ttgcccacga
     1081 agcaaaccgc acgcagcgaa agatttcttt gacagaagat gtagcaaaag cattgatcgg
     1141 cagtgtgacc tatggaaata taggacaatt gaagtcaaat atccagttag tatgtgcacg
     1201 aggatttcta aatcatatgc aatcacccga aatctcgatc acaatcgatg atttgacaga
     1261 agggattcgt agcggattga ttcaattagc aagcaaccgc agtgcaatgg cagagctttc
     1321 taaaatattg gagccaaaga taactgtagc accaaatgat acgatcatga aaatccaatc
     1381 ggactcctat gagttacctt ataatctata tgatatcatc ggagataaag cagctttact
     1441 gaagtcggat ggtttggatc aagaagcaat caatcatttc atttctacag atatcaacat
     1501 ccatctgaag tctttctata aagaccatgg tttttctttt aatgcagaca gcaaattagc
     1561 agaatttgta gatccgaaaa tcatagaagt gaccaatcag atttatgtaa tggtcaaaag
     1621 atctttacca tatgagtttc agcaaaactt tatttatgct atgagcttgc atatcagttc
     1681 ttttttgaaa aaaatcaact taggagaaga acggcatacg aatgataaca tccgtgagat
     1741 ggcttttgat tttccagatg aatatgctgt tgcgaaagaa gtgcgtagtt atatcgaaac
     1801 gtattttcaa gtggagattc cagaaagtga agactattac ttgaccgttc tactcgtctc
     1861 gttacgggca aatcaagcaa gcggcagaat cggtgtagtc gttgcagctc acggaaacag
     1921 cacagcaagc agtatggtcc aagttgtcca acagttgtta gatgcagaga acgtacgagc
     1981 agtagacatg cctttggata tggatccgaa aacagcattg gaccgaattg aacgcaatgt
     2041 acaagaagta gatgaaggaa gcggggtcat actcttagta gatatggggt cccttgcgtc
     2101 gtttaattcg caaattcaaa gagacactgg aattccagta cgtactgttg atatggtaac
     2161 aacttctcta gttttggaaa ctgtacgaaa ggtctcagta ttagggacag atctagatat
     2221 gttgcatgaa tcattgaaaa attttcgagg ttacgcagaa atatcttctg tagaatctgt
     2281 tcaggaagca aaacctattc ataagaaaaa agctgttttg gctatctgcg catctggcga
     2341 aggtacagca caaagaatca aagaattgat cgaacgagcg gtaaacaagc gtcaagaaac
     2401 agaattagaa gttctagctt tgtcgattgt ggaagtgaaa gaagaactgc cagttatcca
     2461 aaaagaatat cagatcatag ctactactgg gattactgat ttgaatatcc aagccccatt
     2521 tattccttta gaacgtttta ttgatcaaaa tatcgaagtc attttggatc agttgctgat
     2581 ggaatcagaa ttggaggaaa ctgaattcat ctccctagat gaagagtcag ctaaaaatac
     2641 gtgtgttgag tttatctctg ataacttcat ctttattaat ggtagtaagt taatcgatcc
     2701 aatgtggcaa ttttctacac aaatctctca gacgacggga atcggcgatg aagaatatgg
     2761 attcaaaatc aacctagtga tgcacacagc aggaatgatc gaacgtatta ttcggaatga
     2821 accattgact gtggaagaaa acgaattgac aaatacaacg aatgatcctc tttatagcca
     2881 attggcagcc tcagttgttc tgttggaaga tcagataaaa gtaaaagtgc cgatagaaga
     2941 aatgtattat ctgctacgtc tagtccataa tcagttagat aaaaaagaat acactgtgcc
     3001 atgaatacac aaaaacgata cacttttata gtgtatcgtt tttgtgtatt ggttaagaat
     3061 cctttaaatt caattagtgt atcgtaaaaa agttggcacg ctttttgctt atagtagtgt
     3121 gtgctaggag gtggttgttc acacagaaat gataggggtt tctgtgaagt agtgaggaag
     3181 aaggagtttt gcagatggat attcgattgg cacgtataga tgatcgttta atccacgggc
     3241 aagtcgcaac agtttggtcg aagcaaacag gtgttgaacg tatcattgtg atcagcgatg
     3301 tagtcgctca agataaattg agaaagtttc tactaaaaga agctgcaccg ccggggatca
     3361 cagcaaatgt aataaccgtg cagaaaatga ttcaaatcta tcatagtgga ttattatcac
     3421 gagcaaaagt gatgctttta ttcaccaatc cccaagatgt tgaagcggtt gtccgctgcg
     3481 gtgtacagtt gaagtcgttg aatatcggcg gtatgagttt tactgaaggc aaatcaatga
     3541 ttaccaattt tgtttcagta aatcaacaag atattgattc ctttcgttac ttgaacagtc
     3601 aagaagtgga gttagagatt cgaaaagtac ctgctgacag gaaaatcctt ttgatggaac
     3661 tactagcgaa aatcaaaaag gtgtagcaag aatggttgtt ggaaaaggtc aaataattct
     3721 gttgtaatgg ttagcaatta ttaatatttt tgaatttgtt acaacaaaag taaataggag
     3781 gtataaatat ggtagggatt atcctagcaa gtcatggaca atttgctgaa ggaatcttac
     3841 aatccggttc aatgatcttt ggcgaacaag aaaacgtcaa agccgtgatt ttgaaaccaa
     3901 gtgaaggtcc agatgacttg agagccaaat tggaagaagc ggttgcttct tttgataatc
     3961 aagacgaagt gttattctta gttgatttat ggggcggaac accgttcaat caatcaaaca
     4021 cattattcga agagcacaaa gataaatggg caatcgtcag cggtttgaat ttaccaatgt
     4081 tgattgaagc ctacgcatca cgcttttcaa tggaatctgc tcatgaaatt gcagcacaca
     4141 tcattgaaac agcaaaagac ggtgtaaaag taaaaccgga agaattagaa ccggcagaag
     4201 caccgaaagc agcagttgaa gacgcacaac ctaaaggagc acttccagaa ggaactgtgg
     4261 tcggagacgg aaaaatcaaa tatgtattag cacgtgttga ctcacgtctg ttacatggac
     4321 aagttgcgac agcttggaca aaagcagtgc aaccaaaccg tattatcgtt gtttcagatg
     4381 cagtatcaaa ggatgatctg cgtaagagac taatcgaaca agctgctcct ccaggtgtaa
     4441 aagcaaatgt tattcctatt agcaaaatga tcgaagtagc aaaagaccct cgttttggaa
     4501 ataccaaagc attgctttta tttgaaaatc ctgaagacgt attgacagct gttgaaggtg
     4561 gcgtagacat caaagaatta aatgttgggt caatggcgca ttcggtcggt aaagtcgttg
     4621 taagtaaagt attgtctatg ggacaagaag acgttgaagc gtttgagaag cttgaacaaa
     4681 aaggtgtgaa attcgatgta cgtaaagtac ctaatgattc acgcgataac atggatgata
     4741 ttttgaaaaa agcaaaagct gaattagcaa aagcgtaata aataatatta aaatttatag
     4801 gaggcttatc atgtctatta tttcaataat tttagtcgta ttagttgcct ttctagctgg
     4861 tatggaaggt attcttgatg aatttcaatt tcatcaacca ctagtggcat gtacattgat
     4921 cggtttagtt accggtaacc tcgaagctgg tatcgtactt ggcggtacgc ttcagatgat
     4981 cgctttaggt tgggcaaata tcggtgccgc tgtagcacca gatgctgcat tagcatcagt
     5041 agcatcagca attattttag ttttaggtgg acaaggtgtt aaaggtgttc cttctgcgat
     5101 cgcgattgcc gttcctttgg ctgttgcagg gttgttctta acaatgatcg tacgtacaat
     5161 cgctgttcca atcgttcatt tgatggatgc ggctgctgaa gaaggaaaca tccgtaaggt
     5221 tgaaatgtgg cacatcattg ctgtatgttt acaaggggtt cgtatcgcgg tcccagcagc
     5281 agcgttgcta tttatcccag cacaaactgt gcaatcattc cttgaatcaa tgccagcatg
     5341 gttaacagat ggtatggcta tcggtggggg aatggtcgta gctgtaggtt acgctttggt
     5401 aatcaacatg atggctacaa aagaagtatg gccattcttc gttattggtt tcgttgtagc
     5461 tgcaatttct caattaacat tgattgctct aggtgcttta ggtgttgcat tagctcttat
     5521 ttacttgaac ctttctaaaa tgggtggttc atcaaataat ggaggcggaa gcaattctgg
     5581 tgacccttta ggcgacattt tgaatgacta ttaatcttga aggaggaaaa taaaatggca
     5641 gaagaaaaaa tcaaattatc aaaaaaagat cgtttagctg ttgcgtggcg ctctaccttc
     5701 attcaaggtt catggaacta cgaacgtatg caaaacggtg gctgggcatt ttctatgatt
     5761 ccagcaatca aaaaattata caaaacaaaa gaagaacgtt ctgcagcatt aaaacgtcac
     5821 ttggaattct ttaatacaca cccatacatt gcttctccaa ttttaggggt tacattggct
     5881 ctggaagaag aacgtgcgaa tggcgcgcct gttgatgatg tagcgattca aggggttaaa
     5941 gtaggtatga tgggtccttt agctggtgtt ggtgacccag tgttctggtt tacagtacgt
     6001 ccaatgctag gtgcactagg tgcttcatta gctatgggag gaaatatcct tggaccaatc
     6061 atcttcttct taggttggaa cattattcgt tgggcattca tgtggtatac acaagaattt
     6121 ggctacaaag ctggttctaa aattactgat gacctttctg gcggattgtt acaagacgta
     6181 acaaaaggtg cttcaatttt agggatgttc gtattagctg cccttgtaca aagatgggta
     6241 tcaatcaaat tcttaccagt tgtatcaact gtaaaattag ataaaggtgc ttacatcgaa
     6301 tgggataaat tgccttcagg tggtgaagga atcaagagtg catttgaaca agttaataat
     6361 ggcttagccc tttctcctga aaaggtaaca acattacaag acaacttaga ccaattgatt
     6421 cctggtttag ctgcattagg attaacattg ttatgtatgt ggttgcttaa gaaaaaggtt
     6481 tctccaatcg ttatcatcct tggtttattc gtagtcggtg ttgttggaca cgtcatcgga
     6541 cttttataat ccataaaaat atagctcggg cgttgtcccg agcttttttt atatagtata
     6601 ctgttcataa acaaatatct ggagggagaa aatggttcaa tcaatcaata caaaagtaga
     6661 cttggtaatt gatgctactt cgtttacggg tctgacagat tacgggcaaa tcatgatagg
     6721 taataatggt tttgagtttt accatgcgcg agatagacgc aaatatatcc aaattccatg
     6781 ggaagaagtg gattatgtac tagcatcagt catgctaaag ggaaaatgga ttcctcgata
     6841 tgccattcaa acgaaaaaaa atggtacata cacattttct tccaaagaac ctaaaaaagt
     6901 gcttcgtgcg atcagagaat atgtagatga agatagaatg gtacaatcat tgagtttctt
     6961 tgatgtagtg aaacgtggta ttcgtagtat gtttaaacgc aagtaaaaaa taaagaaaac
     7021 ttctaagagg aggttttctt tattttttta ctttgcagaa gaaaatatga aatagcaaaa
     7081 ccaattattt gtccccttta aatcccatat gctaaaattg attagcaaag aaggaggtta
     7141 tattatgagt tggttatgga cattgattgt tggagccgtg atcggagcaa ttgcaggtgc
     7201 gatcaccagt aaaggaaatt cgatgggctg tgtaacgaat atcattgctg gactagtcgg
     7261 ttcaagtatt ggacaagcga ttttcggcga atggggacca aaagctgcag gtatggcact
     7321 tattccatct attttaggcg cagtgatttt ggtagcagtt gtctcatttt tctttggaaa
     7381 acgatcataa gtttttacaa aatagtaaac taaaaggagt tgcagcgaga ctgtgactcc
     7441 tttttggctt tatttattta aaataaaaag aagttttccg gaaattttta tttaactgtc
     7501 gtgtacgaag aaaagtttgc tataatggaa aaaagcgtgc aaagaaagag ggagtttcat
     7561 tatgatgcta cgtgtagaaa aattgcgcaa aaaaatgcaa gaagaaaatt tggattcatt
     7621 tttggttacc agtccgtaca atcttcgtta cttaacgaac tttacaggta cgacaggatt
     7681 agctgttatt actttggaaa aagcattttt tattactgat tttcgttata ccgagcaggc
     7741 ggcagcgcag gctcaagggt ttgaaattat taaaaatgtc ggacctattt ttgatgaggt
     7801 agcaaactta gtacgcaaag aagaactgca ctcattagct tttgaggaaa caacagtctc
     7861 atttttagaa tattcggttt tagaagaaat catcgatgcg gaattagtac ctgtttcaca
     7921 gatgatcgaa gaactcagag aagtgaaaga tgaagaagaa atcgctatta tcgaaaaagc
     7981 atgtcatatt gcggatatgg catatgatca catcctgaaa atgatccaac cgggcatgac
     8041 tgagattgaa gtcgcaaatc aactagactt ttatatgcgt tctcttggcg cttcaggtgt
     8101 ttcttttgat acgatcgttg ctagcggatt gcgttcggct atgccgcatg gtgtagcaag
     8161 caaaaaaatc atcgaacaag gtgacttgat cacattggac tttggttgct attacgaagg
     8221 gtacgtttcc gatatgacac gtacattcgc tattggtgat cctggggaaa aattaaaaga
     8281 aatttatcaa atagtcttag atgctcaatt agcagttatt gatgctgcaa aaccaggaat
     8341 gactggtgtc caattagatg cagttgctag agattatatt tcaaaacatg gttacgggga
     8401 agcatttgga cattctacag gacatgggat cggtttagag atccacgaag gaccaaacgt
     8461 ttcttctcgt gcagaaaagc aatttgtacc aggaaatgtg atcactgatg aaccagggat
     8521 ctacttacca ggaatcggtg gagtacgtat tgaggatgac ttgttgatca cagaagatgg
     8581 aaatcgtgtg ttgactcgtt caccaaaaga attgatcatc ttatagtttc atcaatagaa
     8641 gaagtaagcg taaaaatcag tgttcacaga ctttgttata gaaaatcact cgatttaatg
     8701 atacactata gaagaacttt taagctgtaa gagataataa agttgggtgt tttctccaat
     8761 tcatagcagt ttgcagctga tcggcggcag ttttcttacc acgtgatcac tatgccgcat
     8821 catagagaat tcggatgtat ctcgtgctta ggcaaaggga ggaataaata aatggcagat
     8881 gaaaaaaatt tgattttaaa cgcaaaccaa gatttaggag agatcgtgat cgctcctgaa
     8941 gtcatcgaag taattatcgg gattgcagct tctaaagtag aaggcgtata cggaatgcgc
     9001 ggaacttttg ccagcaatgt cactgaatta ttaggacgtg cagctcacgg aaaaggtgtt
     9061 tacttagcaa acgaggaaga tggcttgaaa gtagatcttt actgttatct agaatatggt
     9121 gtttcagttc ctaaagtagc aatggaaatg caggaacggg tcagccagca agtccttttc
     9181 atgacggata tcgatttatc tgaagtcaat atccatgtag tagctgttgt tcctgaaaaa
     9241 ttagaacaac caacaatcga agaacttttt gaagacgaag aggaagagaa tgaataaaga
     9301 attatcaaga cacgagattc gggaaatggc attgcaggct ttatttccat tagacttcaa
     9361 tgcagaccta acaaaagaag atgcgatttt caacgcaatc gaattagacc accgcgacat
     9421 gatcaacgaa gatgaatcag agtttgttcc tgtttatctt gatacgctag ttggcggtgt
     9481 ctgtgcaaaa aaagatgaat tggataaagt gattgaaaaa catttaaaaa acaattggca
     9541 catcaatcgg atctcaaaaa tggatttggt gatcttgcgt atcgcgattt ttgaaatgac
     9601 ttatgtgaca gatgtaccag cggctgttgc tttaaatgaa gcaatcgagt tggcaaaaac
     9661 atttagtgat gaccgttcac gtaaattcgt caacggtgtt ttatccaatg tattgaaaga
     9721 at
//
LOCUS       DAYYGO010000103         9714 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000103 DAYYGO010000000
VERSION     DAYYGO010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.105"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            33..656
                     /locus_tag="ACHA5T_002240"
     CDS             33..656
                     /locus_tag="ACHA5T_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887793.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1106..2155
                     /gene="trpX"
                     /locus_tag="ACHA5T_002241"
     CDS             1106..2155
                     /gene="trpX"
                     /locus_tag="ACHA5T_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8887794.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2152..3033
                     /locus_tag="ACHA5T_002242"
     CDS             2152..3033
                     /locus_tag="ACHA5T_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8887795.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3036..3806
                     /locus_tag="ACHA5T_002243"
     CDS             3036..3806
                     /locus_tag="ACHA5T_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8887796.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4085..4939
                     /gene="aroE"
                     /locus_tag="ACHA5T_002244"
     CDS             4085..4939
                     /gene="aroE"
                     /locus_tag="ACHA5T_002244"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFM8887797.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5117..6280
                     /locus_tag="ACHA5T_002245"
     CDS             5117..6280
                     /locus_tag="ACHA5T_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM8887798.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6397..6807
                     /locus_tag="ACHA5T_002246"
     CDS             6397..6807
                     /locus_tag="ACHA5T_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8887799.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6981..9209
                     /gene="topB"
                     /locus_tag="ACHA5T_002247"
     CDS             6981..9209
                     /gene="topB"
                     /locus_tag="ACHA5T_002247"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM8887800.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9380..9664
                     /locus_tag="ACHA5T_002248"
     CDS             9380..9664
                     /locus_tag="ACHA5T_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887801.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
BASE COUNT         3371 a         1647 c         2087 g         2609 t
ORIGIN      
        1 aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat
       61 cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt
      121 attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt
      181 tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa
      241 ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg
      301 cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt
      361 ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt
      421 atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt
      481 tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt
      541 ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag
      601 aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca
      661 ttggcgcgat gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg
      721 tttgatcttc aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt
      781 cgattagact taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt
      841 atttcagcag aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg
      901 agactttccc atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga
      961 catgaacatt gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga
     1021 ccgctttggc cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa
     1081 agattgactt tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat
     1141 tattttagtt tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag
     1201 tacttcgacg aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca
     1261 tccggcatta gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa
     1321 agaaggagaa aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc
     1381 cacaatgagt caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc
     1441 tgccgctcaa tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac
     1501 ggatcctgtg ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg
     1561 tgtttctgat cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa
     1621 tgcaaaaaaa gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc
     1681 acaagcagaa gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc
     1741 cactaatgag attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt
     1801 tccattagat aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc
     1861 aaatatcccc gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat
     1921 cgggatcaat caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag
     1981 tggaaaagaa aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat
     2041 caatcaatct caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc
     2101 caaaatcatt acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta
     2161 tcagctattt cacaaggcat gctttgggca atattaggat tagggatttt catgacttat
     2221 cggatcttag actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc
     2281 tgtgtgacag ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt
     2341 gctggcatga tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt
     2401 attttagcag ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga
     2461 acaccaaatc tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg
     2521 ttacctgatt actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct
     2581 gtactgctat tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac
     2641 gaaacgatgg ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact
     2701 ttgtcgaacg gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca
     2761 gatgtaaata aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa
     2821 gtgatcttcc aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc
     2881 atttatcagc tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag
     2941 ttgttctccg ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg
     3001 aacttaaaaa cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa
     3061 atggcagaaa agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta
     3121 accttacaat cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa
     3181 gcacactgtt caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta
     3241 aagatcagct gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt
     3301 tccaagaccc caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg
     3361 cacaaaaaag aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag
     3421 attttttcag actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag
     3481 cgggtgaatt atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag
     3541 ctcctgagtt actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag
     3601 cattaatgcg attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca
     3661 cgcatcgaat ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga
     3721 aaatcgttca agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc
     3781 agttctttga agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact
     3841 tgaaaactca ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa
     3901 catatttggg tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt
     3961 ctttgctctg acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc
     4021 gtactttttc gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa
     4081 taagatgatt tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag
     4141 tttatcccca ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt
     4201 agctttccaa gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga
     4261 tatgttaggt gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga
     4321 ggtaagccag gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg
     4381 tttgattggc tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat
     4441 ttcgatcaaa aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac
     4501 tgttcaagct gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac
     4561 attcgctcaa gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa
     4621 agttcgtgac tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct
     4681 agtcaatgca acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat
     4741 aagcatgatg catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg
     4801 tttattaaaa gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat
     4861 ctaccaagga gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat
     4921 tgaaaagctg tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg
     4981 gtctcaagaa aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat
     5041 tatcaaaaaa tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa
     5101 acgaggagga accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa
     5161 acctctgaag cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat
     5221 ggaaaatgag gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt
     5281 gaaccaatac gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa
     5341 taaaaaattt ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc
     5401 gtggaaaaaa atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt
     5461 ttttattggt aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac
     5521 ctttcatggg caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac
     5581 acgcggagaa gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc
     5641 ccataagccg aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg
     5701 acaaaataag atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt
     5761 agtagaaaat tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga
     5821 aaaagtgatc gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa
     5881 tctggatggt gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca
     5941 gtacgcacgt ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca
     6001 agtaatgaac gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta
     6061 tgcagctggt aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa
     6121 aaacctcttt tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga
     6181 cggttcgtgg aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca
     6241 aatgaatcaa tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag
     6301 aagctgcgca aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc
     6361 ttatggtaga tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa
     6421 aatgagtcgc gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt
     6481 atcaccacat acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa
     6541 aatctaatca cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg
     6601 ccagaaggaa ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca
     6661 agaaatgaag aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag
     6721 atgccactgg atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc
     6781 atcaactggc agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa
     6841 gcagcaaacg gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt
     6901 atcttttttt tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat
     6961 taagtaacgg aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa
     7021 aagcctatgc cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg
     7081 atccattgtt tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg
     7141 ttgctccagg agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt
     7201 tcccagaaac cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa
     7261 aacgagaatt gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg
     7321 aagccatcgc gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt
     7381 tacgtttatg gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc
     7441 aacctggaaa gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt
     7501 ggctgattgg aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg
     7561 atggcagttt ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc
     7621 aagaagaaat caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga
     7681 caaacaaagg gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag
     7741 agttaacgaa ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc
     7801 ttgaagttac gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt
     7861 tgcagacaaa gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc
     7921 aaggattgta cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag
     7981 aaaatgagca tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag
     8041 aagaagtaca gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc
     8101 aagaacatca tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc
     8161 tttccaaatt acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg
     8221 ctgcagatta tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa
     8281 aaagcatcgg aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa
     8341 aaaagaaaga agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac
     8401 tgaaaagtat ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca
     8461 cagcaatgaa aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag
     8521 aagtagaagg aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga
     8581 agcattacat cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt
     8641 gtaaggcagt agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag
     8701 ggtacttgag aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca
     8761 aaaaattcat cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt
     8821 cagatgagat tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag
     8881 gaagatgtcc aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct
     8941 tgaatccgga atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa
     9001 cgaatctcag agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg
     9061 gtaaaaaagg gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt
     9121 cttttccatg gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa
     9181 gaaaaaataa tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt
     9241 ccgaaaagtg agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa
     9301 cgttggggta ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca
     9361 ttcattttta aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct
     9421 aaagtatcct gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc
     9481 accaacgttg attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca
     9541 taaaacgaca agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc
     9601 ggctatgatc attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg
     9661 ttaagaatac gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtc
//
LOCUS       DAYYGO010000104         9503 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000104 DAYYGO010000000
VERSION     DAYYGO010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.106"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(152..562)
                     /locus_tag="ACHA5T_002249"
     CDS             complement(152..562)
                     /locus_tag="ACHA5T_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8887802.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            837..1049
                     /locus_tag="ACHA5T_002250"
     CDS             837..1049
                     /locus_tag="ACHA5T_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein-bridging factor 1 family protein"
                     /protein_id="HFM8887803.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            1052..1264
                     /locus_tag="ACHA5T_002251"
     CDS             1052..1264
                     /locus_tag="ACHA5T_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887804.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            1404..1559
                     /locus_tag="ACHA5T_002252"
     CDS             1404..1559
                     /locus_tag="ACHA5T_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887805.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGKKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(1556..1780)
                     /locus_tag="ACHA5T_002253"
     CDS             complement(1556..1780)
                     /locus_tag="ACHA5T_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFM8887806.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            1858..2046
                     /locus_tag="ACHA5T_002254"
     CDS             1858..2046
                     /locus_tag="ACHA5T_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887807.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            2185..2313
                     /locus_tag="ACHA5T_002255"
     CDS             2185..2313
                     /locus_tag="ACHA5T_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887808.1"
                     /translation="MAREEALKIGKVIADNRWGNSRPIILSKQHIEKQKAWQQIKK"
     gene            2372..2707
                     /locus_tag="ACHA5T_002256"
     CDS             2372..2707
                     /locus_tag="ACHA5T_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887809.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            2940..3881
                     /locus_tag="ACHA5T_002257"
     CDS             2940..3881
                     /locus_tag="ACHA5T_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda-exonuclease family protein"
                     /protein_id="HFM8887810.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            3883..4773
                     /locus_tag="ACHA5T_002258"
     CDS             3883..4773
                     /locus_tag="ACHA5T_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFM8887811.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            4816..5616
                     /locus_tag="ACHA5T_002259"
     CDS             4816..5616
                     /locus_tag="ACHA5T_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFM8887812.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADDIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            5631..6482
                     /locus_tag="ACHA5T_002260"
     CDS             5631..6482
                     /locus_tag="ACHA5T_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8887813.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            6683..7144
                     /locus_tag="ACHA5T_002261"
     CDS             6683..7144
                     /locus_tag="ACHA5T_002261"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8887814.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            7170..7622
                     /locus_tag="ACHA5T_002262"
     CDS             7170..7622
                     /locus_tag="ACHA5T_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887815.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            7728..7922
                     /locus_tag="ACHA5T_002263"
     CDS             7728..7922
                     /locus_tag="ACHA5T_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887816.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNEFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            7953..8246
                     /locus_tag="ACHA5T_002264"
     CDS             7953..8246
                     /locus_tag="ACHA5T_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887817.1"
                     /translation="MKLKDGFYSSGHGIGGLMLDMPTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVVRLVDMEVIKS"
     gene            8177..8716
                     /locus_tag="ACHA5T_002265"
     CDS             8177..8716
                     /locus_tag="ACHA5T_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFM8887818.1"
                     /translation="MVSEKQREFSSGEIGGHGSYKILILLFYNYPTTMSVIPQLEGEE
                     AIVNILENIDIKQTRKNARRLLKRYRNLERLVGPVKIDFSVMTVTKNLKFTIDSQNEE
                     IIEAISTRDSVIEALTRLSRIHFQVLYYSYCFPNKMSMYQIGEKLGYSDRTIERMKAV
                     ALVEFAEAYKSGELISRTK"
     gene            8791..9258
                     /locus_tag="ACHA5T_002266"
     CDS             8791..9258
                     /locus_tag="ACHA5T_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HFM8887819.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQHR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
BASE COUNT         3388 a         1504 c         1988 g         2623 t
ORIGIN      
        1 atgttttatt ccctaaatca gtttcaatta aatctatatc agaatatttg attattttat
       61 atggattaat cgcatcagat ccaaactcta caaataattt ttctatttct tctaatatcc
      121 agtatttgtt taaatgcata ccaaagccca cctattgacg atatttttta ggagtgaatt
      181 ttttctttgc tttaactttg gttaaggcaa gtgcaagttg aatggcatcg tataattttt
      241 ccttatcttc atcgtctaaa ggttcaccat aataatttgt ttcagcatct gaattcaagc
      301 cttcaagtaa tttttcagct tgtaaagcaa tatcttgttt atctttttct gttaaatcat
      361 aataatggcg tttatcagta cgtcctaaaa gataatcagt tgatacatta aagtaatctg
      421 caactttttg tattttatct ccagatggag tacttttatt ccatctagac atgcttccat
      481 ttccgaaatt taactttctt tcaagttctg ccaacgtcat tgaacgttct tttaataaga
      541 tttttatacg gtctaatagc ataggatatg cattcctttc aaaataccga caaaaaaagt
      601 atttttctgc aaattatcta tttacaaata gagaatttgc agttatacta gttttgtaag
      661 caagatattt acggcataca aagactaaaa gcaataaact aatagtctcc ccagacatca
      721 gtaagctcgc tttttgtgcg ctgtattttg tatgcttaca taatagcata tttgcagttt
      781 ttgtcaaact tattagacaa aaatattaga aaatattcga taagggggta tgaagcatga
      841 ctttaaaaca gcaagtatta attgctttag taaagaaagg ttggagtcaa agagagttag
      901 ctcgacgaat gggtatatca attacttatt tacgagatat cttcatcgaa aaaagaaaac
      961 ctaaggaacg tttaaaacaa atcgaagaac ttttggatat caagttagaa gtagattcta
     1021 atgatcagcc atcagaacag gaggcgtaaa aatggtacaa cgtttgagca aagcggagtt
     1081 taaagagaaa tataagtatt ctgaaagtac ttatcaaaga agaatgaagg aatttaaaaa
     1141 atcacgtttt agtgaggggt ataacgcagt tacttccaaa gaaataacaa ttgatgtcga
     1201 tttatatgaa cagtttagaa catggcgttc taaaaatcga tttagagtta gagctgtttg
     1261 ttaatagcta aaaaatctaa attcaccact tctgaaaaga ggagttgctg ctctcctttc
     1321 aagtttgcca aagtccacgt tttttcattt tggcaactcc tcttcttgga ggtggtgacg
     1381 gcgagagacg aaggagggat gacatgaagg aagaatcaaa aatagggatt ttgaatgaga
     1441 acaattacac aagattaatt attgaaacag aagaagggaa aaaagtagca gaagttacat
     1501 tgaccgatgc aactcctgct gacggttatc gtattcgatt aactcctaaa tatgattaac
     1561 cttttggtgg gtgtggatcg tttccgtgac tatcacggct ttgaattttt ccatctctgc
     1621 catgaataaa tagctcagat ccttgatttt tagaaatctc tctagcgatt tttactgctt
     1681 ctgattttgt attagtatgt gcagtcgctt tagaatttcc agcacctttg acattccaac
     1741 caccattgga attaggtaca acatgttgat tttttcccat agttttcacc tccatttcta
     1801 tagaaatatt ttatcaaata gaaatggaga aattcaataa acgaaaggag aagtgatatg
     1861 gcatatacct tgcaacaaga acatcaaatt ctcggtttga ttaaacaacg caggaaacaa
     1921 ttacaagatg accgtgcagc gcttagaaaa gccgatgagc tatcagatag gcaagctgaa
     1981 ctaattgctt ctgaacttga ggatttgaga atgctagaaa taaaaaatag ggagattaga
     2041 ttatgaagaa gacagacaca ctttttatag gattcatttt ggggttatta gtgattgtag
     2101 cgcaccaaag tattatcggg ggaagcttgt tcgcagcatt gatggtttta atcaatctgc
     2161 ttgattcaaa agaaaggagc aactatggca cgagaagaag cgctaaaaat cggtaaagtg
     2221 attgctgata atcggtgggg aaatagccgc cctattattt taagcaagca acatatcgaa
     2281 aagcaaaaag catggcaaca aataaaaaag tgactctgcc agccagcaaa gagtcacata
     2341 caaatttata aaactaggag aattatatca catgaataag aaaattgaaa atcttatcga
     2401 agaattgaaa cgtgaatgcc aaaagcaagg tgtttctatt atttgtacag cccaaaaaga
     2461 aggggaactt aaaagtctcg tttatggtga gacaacagaa attttacttt gtcttgctat
     2521 gcaagaagaa catttagatg aaaatctccc attgtcagca catattatgc gtagaattgc
     2581 tgtggatgct tacgaacaag cgaaaaatga agaagaaaat caaccttcaa atcatacttt
     2641 tgttattaat aacaaggaag atttagctga tgtgatgact cgtattctca agggggaatt
     2701 tcaatgatga atgaaaaaac aattgatcta ctcattattt tatttttcac gttagtgatt
     2761 gtagcgaaaa gttatgtaat ctttggcttg ttattcgcaa ttaccatgct tgtgctttgc
     2821 gtgttagcaa gcagaaaaga ggttaaacat gaacgagtta gcaaatcttg acaactattt
     2881 aactgatcct gattatgcta aaccaccata tgaagcacca attgatgagg aggatgaaga
     2941 tgagtaaatc taccttagaa atgagccatc aagaatggct tgaagaccgt aagaaaggca
     3001 tcggaggttc ggatgttgga acaattttag gattgaataa atggaaatct ccttatcaac
     3061 tatggctaga aaaaacagga caagtagtac ttgaagaatc aggaagtgag cccgcttatt
     3121 ggggtaatgt tttagaagaa gtggttgcta aagagtttca ggaacgtaca ggcaaaaaag
     3181 tacgcagaag aaaccaagta tttgaacatc cattacatcc atttttaaga gcaaatattg
     3241 atcgggaagt agtgggagaa aatgccattc tggaatgcaa aacagccaat caatttctcg
     3301 gtaaagaatg ggaaggcgaa gaggtaccgc ttagctatct ctgtcaagtt caacattaca
     3361 tgaacgttct aaacaaagac tattgttaca tcgctgtctt aatcggtggt caaaaattta
     3421 tctggaagcg gattgaacga gatcaagagc tgatcgatac aatcactgaa caattagtag
     3481 agttttggga aacaaacgtt cttggaggta tcgagccaat tattgatggt agtcaagcga
     3541 cagctgattt cttgaaagag aagtatgcag atgttgaaga cgttcaaacg acgttaccaa
     3601 ttcatttcga tgaactagtt gaacagaaaa acgaactcaa acggactaag aaagaaattg
     3661 aatcagctat ccgtcaagta gacaacgaga ttatcagcga gctaggtaaa cgtgaagcca
     3721 gtatcggtat cactcaaaaa aacatcatca gttggaaatt tgtccgtacg agacgtatga
     3781 actcgaagaa actagcagag aaatatccag atgtcgcaaa tgatgaagag atttataacg
     3841 ttactgaatc aagaaggcta accgaaaggg agatcaaata atatggcaac aaatgaatcg
     3901 ttaaaaaatc aattggcagc aaagccacag aaacaagttg caccaggtca attaggactt
     3961 aaagctttga tgaatacacc aacaatgaga aagaagttcg aagaggtgct tcatgacaat
     4021 gctaatgctt ttatgtcgaa tgttatgact cttgtatcta atgacagtta tcttgcagat
     4081 agtgaaccga tgtctatcat gagtggtgcg ttaactgctg caacattaaa tcttgggcta
     4141 gataagaatt taggttatgc atatttagtt ccattcaata gtaaaaacaa gcaaacagga
     4201 aaatgggaaa agaaagctca attcatgctt ggctataaag gatatatcca attagcccaa
     4261 cgatcaggta aatacaaagc attaaatgtg atcgaagttt acgaaggaga actaaaaagc
     4321 tggaaccgac tgacagaaga gtttgagttt gatccaaatg gtagaacgtc tgatgaagtc
     4381 attggatatg ttggctattt cgagttactg aatggattca aaaaaactgt ctattggacc
     4441 aaacaagaaa tcgaagctca tcggattgct aacaataaag atcgcgataa gacaaagtta
     4501 agtggtgtgt gggcatctga ttacaatgca atggcacgaa aaactgtttt gagaaatctt
     4561 ctttctaaat ggggaatctt gtccatcgaa atgcaagaag ccaccacatc ggatgagaga
     4621 gtccaaaggg ttcaagaaga cggcagcatt attgctgaaa cagaagttga agaagatatt
     4681 cctgaaagaa aagaagcaga ggttatatct gaagaaaatg aagatgtaca aactggatta
     4741 tttgatgcat ctaatccgcc gttaaacaaa taatgaggga gctttctccc ttgcttttct
     4801 agaggagaaa tacgaatgaa cacaggatat gtcaaattac atcgaaaagt gatgaattca
     4861 ttcgtttgga ccaatcctta tatgtataaa ttatggaact tgtgtttgat gaaagcgagt
     4921 catgagaatc gcaagatact ttttaatgga aaagagatac aagtgaacag cggagaattc
     4981 gtcacagggc gcgatgctat cacatctgag atgaacgaag gcgtgaaacg tgaacatcaa
     5041 gtgaacagcg gttctgtatg gagatggcta aaacaatttg aaaaaaacgg aatgttgaac
     5101 atcaaatcaa ctacgaaata cagcgttata tcaataaaaa actggtcttt gtaccaaagc
     5161 agtgaacaac aaatgaacat caaacgcaca acaagtgaac aacaagtgca cacaaacaag
     5221 aatgaaaaga attataagaa tgaaaagaat aataaaacat catcgtcgca accacgcaaa
     5281 aagcgtgttt acgacaccga ctcagtttac tacattctcg cggaggagtt attcaaacag
     5341 atttgtcaga atcaggaaat caaaaagccg aatctgcaaa gttgggcaga tgacattcgg
     5401 aaaatgatcg agatcgacaa gcggaccgaa aaccaggtac ggggaatgat tgaatggagt
     5461 cagcacaatg tgttttgggc atcgaatatt ttatctgcta aaaaattgcg agaaaaatac
     5521 gacacaatgg cagcgcaagc aaatcgtgat tataaaacaa aacaaactaa aacgcttgaa
     5581 tacgagaaat ttagcacaga tgagttgcct atttgagagg aggcgtaggc atggaaactg
     5641 ttggggaaat catggaaaag ctgatacaga aagtgcttgt ccaacgtggc gaatgtcctg
     5701 aatgcggaca gcctttgtat ggatggcgta cgaagaaccc tgatggctca gaacgttgta
     5761 agccaacctg tatgcaatgt gggtataaag cattgcgagt tcaggaagac ttacaaacag
     5821 aacgaattta caacgagagc ctgaaagcaa gagcaatcaa ttttttcaaa ggtggttctg
     5881 ttgtgcctaa tcaagcgttg tttgattgca cattgcagaa ttatcaaatt gtcgatcaag
     5941 aaacaagaca agcggttgaa gtaaccaaac gctttgttaa ttcagtcttg ttaggaaatc
     6001 caagtcacct tgttttaact ggaaaacaag gaacaggtaa aagtcaccta gcgatggcag
     6061 cggcttggga agtcttgaag cgatcaaact acgataaaaa aatcttattt atcgggttac
     6121 aggaaatgct ggatcaaatc aaattttcgt acaacaatcc tgaactcaga aaaacgattg
     6181 agggatcgtt gattgcagat atcaaaacag cggatttagt catcattgat gatatcggtt
     6241 cagaactagg aaaagatgca tcagatagtc gagcgtttgg cataaacacg ctaaattcgt
     6301 tcttggatgc acgacagaac ctagcaacga ttatcacaac gaacttgctt ggtgaagaac
     6361 tgaaaaaagc ttacggtacg agaacaatat caagaatgtt tgtcaactct gatggattta
     6421 cgatggtatt ttctcaaaca gcagacaagc gcataaaacc agtcaaagga gcgatcgcat
     6481 gaatttgact aaacaagaaa aaagaatctg tcgtcagata atgttgcaaa ttataggtga
     6541 tcgtgacatg gacgaattat ctcaacaaga gagtcgaatg gttcggaaga taagtaagct
     6601 aattatttat cgggatacag aaagatttct cactgtttta ggtaacgtct acgagaatcc
     6661 agagttattg gaggtagagt aattgccaaa aatattagat gcatgttgcg gtagtagatt
     6721 gttctggttt gataagaaca attctgacgt aacatttatg gattgtcgtc agcagtatga
     6781 agaattatca acaggtcatg tgatcaatgt tgatccagat gtggtcgcag actttcgcga
     6841 tatgcctttt gaagatgatg tgttcgatat ggttgtattt gacccgccac acttaagaca
     6901 tgtcggcgaa aactcatggc ttgccaaaaa atatggaaaa ttagacgaac tttggccaga
     6961 agacatcagg caaggttttg cagagtgtat gcgagtttta cgaccttcag gaacattaat
     7021 ttttaagtgg aacgaggaac agataccact ttctgatgtg ttagaggcta ttggagaaca
     7081 gccattgttt ggtaataaac gaagcaaaac acattggctt gtgtttatga aaaaattcag
     7141 ctaacggagg aaacaaagga ggaaaagtaa tgaagattgt attaaataaa tgttttggtg
     7201 gctttgggtt gagccatgaa gcaaaaatgg aaatttttaa aagaaaaaat attgaagtat
     7261 tcccatacat caataacttt agctatgact cagatgatga atacacgaga tacacaggcc
     7321 aaaagctagg ttcgatggat tttatctact atttcaaaaa agacccgaaa atcgacaaag
     7381 ttacaggaat ttatagcgaa attgaccgac tgtacggaat tgctgatgat tccagttttt
     7441 caagtgattc aaacagaggt gataaggacc tcgttgctgt tgtcgaaaaa ctaggaagtg
     7501 aggccagtgg accatatgca agtcttaaag tcgttgatat acccgatggg gctgagtggg
     7561 aaataagtga ttatgacggc gttgaaactg cacattatgg ttttcaaaca ggtagtattt
     7621 agtccactaa tgacaactaa agaggaggca gaaggatgag tgaagatgaa tactttgaac
     7681 agtagtctat taacgacaga gatttttaac tggaaagggg tgtagtcatg gacgcatcgg
     7741 aagtagtaaa taaaattaat agtctcaaag aggttttcgg agacgtcgaa gtagtgataa
     7801 acgcacaagg ctatgacaac gaatttttta agcagattac cgacattagc attcagcaag
     7861 gaatggaaga cgaagacgga aactttatcg atgaagtagc cattttagtg acttgcgagt
     7921 aagtaattga cagctaaaga ggaggaagcg gaatgaaact aaaagacgga ttttactcca
     7981 gcggccacgg catcggcggt ttaatgctag atatgccgac aaagaatcct aaaacacgta
     8041 agaaaccaaa attcaaagtc ggtgacatgg ttcgctgcga agcagaagga ttcatctatc
     8101 catttcgtgg atatgtagaa cacgtctata atcactcagc aatcattcgc attgaaaaca
     8161 cgatggaatg tgataagtgg ttagcgaaaa gcaaagagaa tttagcagtg gtgagattgg
     8221 tggacatgga agttataaaa tcttaatttt gcttttttac aactatccta caacaatgag
     8281 cgttattcca caactggaag gagaagaggc aattgtgaac attttagaga atatagatat
     8341 taagcaaaca aggaaaaatg ctagacgatt actaaaaaga tacagaaatt tagaacgttt
     8401 ggtcggacca gtgaaaatag acttttccgt gatgactgtt accaaaaatt tgaaattcac
     8461 aattgacagt caaaacgaag aaatcattga agcgataagt actcgagatt cggttatcga
     8521 agcattaaca cggctaagta gaatccattt ccaggtactt tattatagtt attgttttcc
     8581 taataagatg tcaatgtatc aaataggaga aaaattagga tattccgata gaactatcga
     8641 gagaatgaag gcagtagctt tggttgaatt tgctgaggcg tataaatcag gagaactcat
     8701 ttcacgtaca aaataaaaaa agtcggattc ctccgactat gaataatatt tccgacataa
     8761 gtattatatc ataattgggg gaatcagagg atggtacttt tcgatgtaaa gaaatatgag
     8821 acaccaagcg cgaaagatgt tgatatggaa cgcacaaaac ataatgtcgc tgtgtttctt
     8881 tcagcatatc tatcagctag atgtagagta gggcaacatc gagaaccaaa agtgacagca
     8941 tcttattcct tggttccacc ttctacagct gatcatatat ttgaagcaga aagaatgctg
     9001 atcgataaag aagaagcaca ggaagaattt gagtatctgc ataaattgtt tgttcgaggc
     9061 tattcagcga tacagcatcc gcataagcct gatgtgactg aaaggcgcaa gaagatattc
     9121 tatgatcgtt atatcaatgg tctgcccatt tatgtaactg ctcaaaggaa taatactagc
     9181 gaagaatcgg ttaaagtaga atcaaacaga attatcatcc aatttgcttc atcgttagaa
     9241 ctggttgctt tcaagtagcc agtttttaca ctttttatac ccttttatta ccaatttggt
     9301 ttccatttta tacctttttt ataccaatca cttacctatt caacgttgta ttatgatagt
     9361 gtcgaaaaat taggaaacag gatcgacaaa ataaaatgta agggaggaaa tctccctcat
     9421 cgtttaatta agcttcgata ggcagcaacg gaaatattaa gaataaggat gtgaatttca
     9481 actccttcta aattgttctt att
//
LOCUS       DAYYGO010000105         9432 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000105 DAYYGO010000000
VERSION     DAYYGO010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.107"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..680
                     /locus_tag="ACHA5T_002267"
     CDS             <1..680
                     /locus_tag="ACHA5T_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8887820.1"
                     /translation="YFSRFSLATREAVQWIVIDMYAPYVSLVKKLFPNAQLIIDRFHI
                     VQHIGRTFLNHRVKETTARLKGTSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRAS
                     FKDYLTESEIVDRLLSACPNLRQGYQLYQDILYAVKKRDQSLFEDCLNREVSGLPETY
                     TTTLRTFKKFLPQIQNALHYSYSNGPLECLNNHIKVLKRNAYGFRSFYNFKLRIMIRH
                     GKTFLTK"
     gene            800..1000
                     /locus_tag="ACHA5T_002268"
     CDS             800..1000
                     /locus_tag="ACHA5T_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFM8887821.1"
                     /translation="MIEEEQKNIWDRYYKADPSSKNTKIGESGLGLSIVKELVRLHHG
                     SIEVTSRKNEGTTFLIQFPKEK"
     gene            1076..1273
                     /locus_tag="ACHA5T_002269"
     CDS             1076..1273
                     /locus_tag="ACHA5T_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887822.1"
                     /translation="MVECLKNKKMEANFVKAVISGSIVFQEEYRDMEKDFSKKGIELI
                     DYPRSCPILDKEYPEILTTFF"
     gene            complement(1570..3117)
                     /locus_tag="ACHA5T_002270"
     CDS             complement(1570..3117)
                     /locus_tag="ACHA5T_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFM8887823.1"
                     /translation="MNITFLETSDMHGYVSPTDYSGRTDLALGTAKVSSKLKELRAKA
                     DGPVITIENGDFIQGSPLSYYLAKEYGSAKELTDLINQMSYDVQVLGNHEFNYGVDYL
                     EEAIKNYQAPILGANMVNKNDQPYFGNAYKIIEKVGVKIAILGLTTQYIPHWEKPETL
                     AGITFKSIVETAKEYVPLLRKQADLVIVSYHGGFEKDLLTGEDTEALTGENEGYELLQ
                     QVEGIDALFTGHQHRKIAETVHGVPVVQPGYRGEFIGEIRLTVEKKEGQVVVTDHQVA
                     LHSVEKSEPDPEILAAIVPVEEKLEKWLDQPLGKVNGNMRINDPMQARLEEHPYVEFI
                     NRVQLFASGSEISGTALFNNEGKGFGETITMRDVITNYIYPNTLAVIQINGADLRAAL
                     EQTANYLAVENNQIVFNPAFIQPKPQYYNYDMYEGIQYTIDMKQPVGQRITELRFKGH
                     PVLPQQMLEVVTNQYRAIGGGNYAMFGAEKIVREIQIDMTELIADYLNIHPRIEAKVN
                     HNFQVIL"
     gene            complement(3201..4004)
                     /locus_tag="ACHA5T_002271"
     CDS             complement(3201..4004)
                     /locus_tag="ACHA5T_002271"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8887824.1"
                     /translation="MNILHISDIHFRREYEACEEGYKGMLATMQNPLIPLEQCIHHLL
                     QQTPLDLVIISGDLTEDGEIDDYRYLKTWLENALGETTILVTLGNHDIKEHFWVGWCD
                     EAASSAPYNQIVKYPEFTVISFDNSSYGYADGIVDDQQFQWLKKALEQEKDQPIFLVT
                     HHHLLSHQSSIPKWPGTERFLKLIAPYDIRCILNGHTHHTFTDEINGIPYFTVSSMSF
                     VGEDEGDGFVRFEERHGYNLYHLDQGRIVRQTTENFIPGNILKTLQMKD"
     gene            complement(4007..4789)
                     /gene="phnE"
                     /locus_tag="ACHA5T_002272"
     CDS             complement(4007..4789)
                     /gene="phnE"
                     /locus_tag="ACHA5T_002272"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005233297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFM8887825.1"
                     /translation="MMLREMNRRTLLSLVALVILVILSAATLDYSGLSTFSPSMGMDV
                     LRGLGQPDWQFLYDGSGEDLISLLLLTIGIACLGTMIATVLAIPITLISAVNLWQAHP
                     WVTKIGKLICNVLRAFPELVFAIIFVKVVGPGPFAGVMAIGVHQIGMLGKLFTEEMEA
                     MDERLVEEMHAVGANFWQTIFFVRIPYLMPIYCSLALNHFEIAVRSAATLGLVGAGGI
                     GAPLIFAIQTRSWSKVSIILIGVVVTVFILDQITGIIRKRLR"
     gene            complement(4786..5565)
                     /gene="phnE"
                     /locus_tag="ACHA5T_002273"
     CDS             complement(4786..5565)
                     /gene="phnE"
                     /locus_tag="ACHA5T_002273"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFM8887826.1"
                     /translation="MSLKETVHFKWYKHLLILLFLIVCFQGSAWITSADFSQVFQNSN
                     QMTVFLSRFIHPDFSYLPKLVEPMVKTLQMSLLGTVIGLILAIPVSFLATTVVTENRL
                     LSTIFRFLLGLVRTIPTLLLAALFVAVFGIGEATGVLTIAVFTFGMVSQLMFQAIETI
                     DFGPIEAAAAVGANKMQIAIWSIAPQVLSQFASYAFYAFEVNVRASTVLGYVGAGGIG
                     VILNSSLALLNYERVSIIILTILVTVAIVDKLSERVRRSLL"
     gene            complement(5537..6328)
                     /gene="phnC"
                     /locus_tag="ACHA5T_002274"
     CDS             complement(5537..6328)
                     /gene="phnC"
                     /locus_tag="ACHA5T_002274"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFM8887827.1"
                     /translation="MIIFDNVSKVYPNGVVGLKDIDLTIEDGEFVSIIGLSGAGKSTL
                     LRSINRLAETTKGDIRINGTSITKANKKELRQIRRQIGLISQSFNLVKRSTVQKNVLS
                     GRLGYYSTWKSILGLFTKEDYQQTKEALEKVGLSDKLQTRSDELSGGQQQRVSIARAL
                     VQQAPIILADEPVASLDPITTQKVMMDLKNINQTMDKTVIVNLHSVPLAREFSTRVLA
                     LKAAQVVFDGRPDDLTDERLEQIYGNAIFEEKAGEIDELERNRSL"
     gene            complement(6389..7318)
                     /locus_tag="ACHA5T_002275"
     CDS             complement(6389..7318)
                     /locus_tag="ACHA5T_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM8887828.1"
                     /translation="MQFKQLAKGFIGVMLLTTLAACGSGNTSSSSEKESKEKLVVQFV
                     PTNNDGSMEAKAKPFAKYLSKKLDREVEVTLATDYSTIVEAMSSGQVDVGIMPPAAYV
                     QAKDAGAADAILTSQLGDYDQETGLPLKDKLTNTFKGEVLVRADSGLNKLTDLKGKNI
                     ATLSPNSASGYIYPVAELKDAGIDPTADTKMTTVNDIPSEITAVLNKQMDAAFVFEGA
                     RNVFASNFPDNDLFKDLKVLYLTKGDIPNDAIAVQPDMSKDLKAKIKETFLNMKDDKD
                     GKEAMALWGHQGYEEAADSAYDTIRDYTKKAAE"
     gene            7513..8196
                     /locus_tag="ACHA5T_002276"
     CDS             7513..8196
                     /locus_tag="ACHA5T_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HFM8887829.1"
                     /translation="MKKIHDTKQLTAYVEKHNLQDYMDSDILAMASLLFFEKEEHLIQ
                     TGKTSDYLYFLVDGTVMVYSYSSDTQNICIDYAKPATPLGEASSLWELSPKSSVKAVT
                     KCICISIPLNQYRTQLQKDVRFLQNICQLLSYRLNSGINLANSLTEPVETRLAKFILT
                     HHENERFSFRLTTCASILNVSYRHLLRTITNFREENIIEKKKNYYLIHDIEALEDLAE
                     NISSPVKAE"
     gene            8357..9400
                     /locus_tag="ACHA5T_002277"
     CDS             8357..9400
                     /locus_tag="ACHA5T_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8887830.1"
                     /translation="MKKILVSLLFLVSLVTLVGCSQSQGEKQSEKEKSKIALVLGFGG
                     VDDRSFNQAGWEGLEAWGKEKKLTAKKDFTYIETNNPEDNSTNIDQAIRNGFTTIISL
                     SYLQAEELENAAKDNPKLKFALVDGEITGKKNISSATFKDQESAYLAGLAAAYTTKSD
                     QVGFIGGMEGPVIQRFQSGFEAGVKAGATALNKEITVNVQYAASYDSLDKGKAIAASM
                     YKEGVDILYQAAGSTGAGVFQEAKARNENDYSRKVWVIGVDRDQADEGDYKMENGETG
                     NFTLTSTKKNVGAEVKEFAETSHNDKFPGGKHLTFGLKEEGVDLTKGQLSEEAYQAVS
                     DAKEKIISGDFRL"
BASE COUNT         2893 a         1933 c         1869 g         2737 t
ORIGIN      
        1 gctatttttc tcggttttca ttagcgactc gtgaagccgt tcaatggatt gttattgata
       61 tgtatgcgcc ttatgtttca ttggtgaaaa agcttttccc gaatgctcag ttgatcatcg
      121 accgtttcca catcgtccaa catattggca gaaccttctt gaatcatcga gtgaaagaaa
      181 ccacggcacg gcttaaagga acttcccact ctcaacgagg gttagggaaa aaattgaaac
      241 gctattggaa actcctacaa aaagaagaag caaagttaaa ctacgaaaaa agggtttggc
      301 gtgccagttt taaagattat ttgactgaaa gtgagattgt cgatcgactg ttatctgctt
      361 gtcccaacct gcgccaaggc tatcagttat accaagacat tctttatgcg gtaaagaaaa
      421 gagatcagtc attatttgag gactgtttaa acagagaagt cagcggtctt cctgaaacct
      481 atacaaccac tttacgaacg tttaaaaagt ttctgccaca gatccaaaat gcgcttcact
      541 acagctattc aaatggacca ttggaatgtt taaacaacca catcaaagta ttaaaacgca
      601 atgcgtacgg ctttcgcagc ttttacaatt tcaaactacg gatcatgatt cgtcatggca
      661 aaacattttt aaccaaataa acagaacaga aagagagttt cttctttctg ttctgtccaa
      721 attattctaa gatttctctt taccaacatc atttgacaaa gagcctgtat ttattactgt
      781 cgaggacaat ggcatcggca tgattgaaga ggaacaaaaa aacatttggg atcgctatta
      841 taaagccgat ccgtcaagta aaaatacaaa gataggcgag tcaggtcttg gattatcaat
      901 cgtgaaggaa ctagtgcgcc tgcaccacgg ttcaattgaa gtgaccagcc gaaaaaatga
      961 aggaacgacc ttcctgatac aatttccaaa agaaaaataa aattaccagt aaaaaaccgt
     1021 ggagagatag ctgctattga tttttgctat gtcttttctt tgagaggtat ccggtatggt
     1081 ggagtgtctg aaaaataaaa aaatggaggc caattttgtg aaagctgtta tttctggaag
     1141 tattgttttc caagaagaat atcgagatat ggaaaaagat ttcagtaaaa aaggcattga
     1201 attaattgat tatcctcgct catgtccgat cctagacaaa gagtatccag aaatcttgac
     1261 gacttttttt taaatatcgc agcagcggat ttattttttg tatttaatca agatcggaaa
     1321 ggggtattag gctatattgg tgccgcaact ttttcagaaa tgaagtattg tctgatgcaa
     1381 aatcttattc atggaaaaag gatcaaaata gtcttattta aggaaccatc agaggaaaat
     1441 tttttgtttt gatgaggtag atcgctggct aaagaatggc tggatggaaa tcagacagcc
     1501 taacaatttg gcataacaaa ggtgtgggca aaaaactgct cacacctttt tgaatttttt
     1561 atttatatac tacaaaatca cttgaaaatt atgattcact tttgcttcga tgcgtgggtg
     1621 aatattcaaa tagtcggcaa ttaattcggt catgtcgatt tggatttctc ggacaatttt
     1681 ctccgcacca aacatcgcat aatttcctcc gccgattgcc cgatattgat ttgtcaccac
     1741 ttccagcatt tgttgcggaa gaacggggtg acctttaaaa cgaagttcgg taatccgttg
     1801 tccaacaggc tgtttcatat caatagtgta ctgaatccct tcatacatgt cataattgta
     1861 gtactggggc ttaggctgaa taaaggctgg attgaatacg atttggttat tttctacagc
     1921 taaataattt gcggtctgct ccaacgccgc tctcaaatct gcaccattta tttgaatcac
     1981 tgccagagta ttcggataaa tatagttggt gatgacgtcc cgcatcgtga tcgtctcacc
     2041 aaagcctttt ccttcattat tgaataacgc agttccagaa atctctgagc cgctggcaaa
     2101 taattgtaca cgattaataa actctacata gggatgttcc tcaagacgag cctgcatcgg
     2161 atcattaata cgcatatttc cgttcacttt acctaagggt tgatccaacc atttctctag
     2221 tttttcttct accggtacga tcgctgcaag aatttctgga tcaggctctg atttttcaac
     2281 tgagtgtaaa gcaacctggt gatctgtaac aaccacttgc ccctcttttt tctcgacggt
     2341 aagacgaatt tcaccgatga actcgccacg atagcctggt tgaacaacgg gaactccatg
     2401 aaccgtttca gcaattttcc ggtgctgatg accggtgaaa agggcgtcaa tgccttcaac
     2461 ctgctgcaat agctcgtagc cttcattctc tccagttagc gcttctgtgt cctcaccggt
     2521 taacaagtct ttttcaaatc cgccgtggta actaacgatt accaagtctg cttgtttcct
     2581 taatagagga acatattctt ttgctgtttc gacgatactc ttaaacgtaa ttcctgctaa
     2641 ggtttctggc ttttcccagt gaggaatgta ttgtgtcgtt aatcccaaga tagcaatttt
     2701 tacacccacc ttttcgataa tcttataggc gtttccaaag tatggttggt catttttgtt
     2761 caccatattg gctcctaata taggcgcttg ataattctta atcgcttcct cgagataatc
     2821 cacaccatag ttaaactcat gatttcccaa cacttgaaca tcatagctca tttggttaat
     2881 caagtctgta agctctttgg ctgaaccata ttctttagca agataataac tcaaaggaga
     2941 cccttggatg aaatcaccat tttcgatcgt aattaccggt ccatctgctt ttgcccgtaa
     3001 ttccttcagt ttcgacgata ctttagccgt tcctaaggcc agatcggttc tgccggaata
     3061 gtctgttggt gaaacatagc catgcatatc acttgtctct aaaaacgtaa tgttcattct
     3121 gcgtgctcct ttagctgact caaaatagaa aaaccgaagc gtattcagct gttcggtctc
     3181 tttttttgat ttataatgcc ttaatccttc atttgaagtg tttttaaaat gttgcctgga
     3241 ataaagtttt ctgttgtctg gcgaacaatc cttccttgat cgagatgata aaggttatac
     3301 ccatgacgtt cttcaaagcg tacgaagcca tcgccttcat cttcaccaac aaaggacata
     3361 ctcgatacag taaaataggg gatcccatta atttcatcag taaatgtgtg atgggtatga
     3421 ccgtttagga tacagcgaat atcgtaaggt gcgattagct tcaagaaacg ttcggtacca
     3481 ggccattttg gtatactgct ttggtgagac agtagatgat gatgggtgac caagaaaatc
     3541 ggttggtctt tttcttgctc taaggctttt ttcagccatt gaaattgctg atcgtccact
     3601 ataccatctg cgtatccata gcttgaatta tcaaaggaaa tcactgtaaa ctcaggatat
     3661 ttgactatct ggttgtacgg ggcagaagag gcggcttcat cgcaccagcc tacccaaaaa
     3721 tgttctttga tatcgtgatt gcctagagta acaagaatcg tcgtttctcc tagcgcattt
     3781 tccagccaag tcttcaagta tcgataatcg tctatttcgc catcttcggt taaatctccg
     3841 ctgataatca caaggtccaa gggtgtttgc tgcaggagat gatgaatgca ttgctccaga
     3901 gggataaggg gattctgcat cgtagccagc attcccttat agccttcttc acaagcctca
     3961 tactctctgc gaaagtgtat atctgatata tgcaatatat tcattcttat ctcaatctct
     4021 ttcttataat tccggttatt tgatctaaaa taaacacggt tacgactacg ccaatcaaaa
     4081 taatactcac tttgctccaa gaccgagttt gaatcgcgaa tatcaatgga gcaccgattc
     4141 cgccagcgcc aactaagcct aaggtagccg cactacgaac cgctatttca aaatgattta
     4201 atgctaaaga acaataaatc ggcatcaaat aggggatgcg aacaaagaaa atcgtttgcc
     4261 aaaaatttgc tcctaccgca tgcatttctt caacaagtcg ctcatccatc gcttccattt
     4321 cttctgtaaa aagtttgcca agcattccga tctgatggac gccaatcgcc ataacaccag
     4381 caaaggggcc aggtccgacg actttcacaa aaataattgc aaaaactagt tcaggaaaag
     4441 ctcgtaaaac attacagata agcttgccaa tttttgttac ccatggatgt gcttgccaga
     4501 ggttaaccgc actgatcaag gtaatgggaa tcgctaatac cgtagcgatc attgtcccta
     4561 aacaggcgat tccaatggtt aataatagca aactaatcaa atcctcgccg ctgccatcat
     4621 atagaaactg ccaatcaggc tgacctaacc ctctcaaaac gtccattccc atggaaggag
     4681 agaaggtgct taatccagag taatccagag tagcggccga caaaataacg agaatcacta
     4741 aagcaactaa tgataaaagg gttcttctgt tcatttcacg aagcatcata acagggacct
     4801 cctgacacgt tcacttaatt tatcaacgat cgcgacagtt actaggatcg ttagaataat
     4861 gattgaaact cgttcataat tcaaaagcgc aagtgaggaa tttagaataa cgccaatacc
     4921 accagctccg acatatccaa ggacagtaga agcacgaaca ttcacctcaa acgcgtaaaa
     4981 agcatagctc gcaaactgac tcaaaacttg tggagcgatc gaccaaatag cgatttgcat
     5041 tttatttgct ccaacagccg cagcggcttc aatcgggcca aagtcaatgg tttcaattgc
     5101 ttgaaacatt aattgtgaca ccataccaaa agtaaagaca gcgatagtca acacgccagt
     5161 cgcttcacca attccaaaca cagcaacaaa aagcgcggct aaaagcaggg tagggatggt
     5221 ccgtactaaa cctaaaagaa aacgaaagat ggtagaaaga agacggtttt ctgtcactac
     5281 agtggtcgct aaaaaactaa cagggatcgc taaaattaag ccaatgaccg ttccaagcaa
     5341 agacatttga agtgttttca ccataggctc gactaattta ggtaaatagc taaagtctgg
     5401 atgaatgaag cgtgataaaa agacagtcat ttgattggag ttttgaaaaa cctgagaaaa
     5461 atcagctgac gtgatccagg cacttccttg aaaacaaaca attaagaaca gaagaataag
     5521 caggtgttta taccatttaa agtgaacggt ttctttcaag ctcatcgatt tcgcctgcct
     5581 tttcttcaaa gattgcattg ccataaattt gctctaagcg ttcatcggtt aagtcgtctg
     5641 gacgaccatc aaagacaacc tgagcagctt ttaacgcgag cacacgcgta gaaaactcac
     5701 gagccagagg gactgaatgc agattgacaa tgactgtttt atccatcgtt tgattgatat
     5761 tttttaggtc catcataacc ttttgagtcg tgattggatc taatgatgca acgggttcat
     5821 ccgctaaaat aatgggggct tgctgcacga gtgctcgcgc aatggatact cgctgttgtt
     5881 gcccaccact gagctcatca ctgcgtgtct gcaatttatc agacaaacca actttttcca
     5941 gtgcttcctt tgtctgctgg tagtcttcct tcgtaaaaag acctaaaata cttttccaag
     6001 tggagtaata gcccaaacgt ccagatagca catttttctg aaccgtacta cgctttacca
     6061 gattgaagct ttgcgagatt agaccaatct ggcggcgaat ctgtcgcagc tcttttttat
     6121 tcgcttttgt aatagaggtt ccattaatgc gaatatctcc tttggtggtt tctgccaagc
     6181 gattgatact gcgcagcaga gtgctctttc cagcaccact caaccctatg atggaaacaa
     6241 attctccatc ttcaattgtt aagtcaatat ccttcaagcc aacaacgcca ttgggataga
     6301 ccttcgaaac attgtcaaaa ataatcatta tccttctcca tttttttaag tgtaagcaaa
     6361 aaaaagcttg ctgacactta ctgatgtttt attctgctgc ttttttcgtg tagtctctga
     6421 tcgtgtcata ggcagaatca gcagcttctt catagccctg atgcccccac aaagccattg
     6481 cttctttgcc atctttatca tccttcatat ttaagaaggt ctctttaatt ttcgctttta
     6541 aatcttttga catgtcaggc tgtacagcga ttgcgtcatt cggaatatcg ccttttgtca
     6601 ggtacaggac ttttaaatcc ttaaataaat cattatctgg gaaattcgaa gcaaaaacat
     6661 ttcgcgcgcc ttcaaaaaca aacgccgcat ccatttgctt gttcaacact gcggtaatct
     6721 cactcggaat atcattcaca gtggtcatct ttgtatctgc agtaggatca atcccagcat
     6781 ctttcaattc agcgacagga tagatgtagc cgctagcaga gttaggactt aaggttgcga
     6841 tattttttcc ttttaaatca gttaacttat tcaatccgct atcggcacga accaatactt
     6901 ctcctttaaa ggtgttcgta agtttgtcct ttaatggcaa gccagtttct tgatcataat
     6961 cgccgagctg agaagtcagg attgcatctg cagctccagc atcctttgct tgaacatagg
     7021 ctgcaggcgg cataattccg acatcgactt gaccagaaga catcgcttcg acgatcgttg
     7081 aataatcagt agctaacgtc acttctacct cacgatctaa ctttttcgat aaatacttag
     7141 caaatggttt tgcttttgcc tccatactac catcattgtt tgttggaacg aattgaacaa
     7201 ctaatttttc tttgctctct ttttctgagc tgctacttgt attcccagaa ccgcaggccg
     7261 ccaaagtggt caataacatg actccgatga acccttttgc taactgtttg aactgcattt
     7321 ttctcctcct gttggatata atttctaata cgtctaaatc atagcaggga atcaaaatca
     7381 atgaagtgac gcacgtccta ttttacacaa cttttacata caatttacaa acaattataa
     7441 ataattattc gtattgagcc gaaaaacaat ctaagctata ataaagaaaa acttcttaga
     7501 ggtgacgatt tcatgaaaaa aatccatgat acgaagcaat taactgctta cgttgagaag
     7561 cacaacctgc aggattatat ggattcagat atattggcaa tggcttcttt actttttttt
     7621 gaaaaggaag agcacctcat tcaaacggga aaaacctcag attatttgta ttttttagtg
     7681 gatgggacgg taatggtgta ctcgtattct tctgatacac aaaatatttg catcgattac
     7741 gcaaagcccg ctacaccact cggcgaagcc tcttcattgt gggaattatc gccgaaaagc
     7801 agcgttaaag cagtgactaa gtgcatctgt atcagcattc cgctaaatca atatcgaaca
     7861 cagcttcaaa aagatgttcg ctttttgcag aatatttgcc agttactaag ttatcgttta
     7921 aattccggga taaatttggc gaactcactg acagaacctg ttgaaactcg tttagcaaag
     7981 tttattttga cccatcacga aaacgaacgt ttctcatttc ggttaaccac ctgcgcctca
     8041 atcttaaatg tcagttatcg acatctttta cgaacaatca cgaattttcg tgaggagaat
     8101 attatcgaaa agaaaaagaa ttattacttg attcatgata tcgaagcttt agaggactta
     8161 gctgagaata taagtagtcc agtaaaagca gaataacagc tataaaaaaa cagcagtcag
     8221 aagtgattgc tggttttttg atagtggaga aattgggaaa cggaaaatct tcgttgaaga
     8281 gtagtgttct atatattggt atgctatact tttaaaagtt caatgtgtca agacacataa
     8341 aaaggggaga aaaattttga agaaaatact tgtttcattg ttgtttttgg tgtcattagt
     8401 aacgttagtc ggctgtagtc agtctcaggg ggagaagcaa agtgaaaaag agaaaagtaa
     8461 aatcgctttg gttcttggat ttggcggggt tgatgaccgc tccttcaatc aagctggctg
     8521 ggaaggatta gaggcttggg gaaaagagaa aaagctgaca gcaaaaaaag attttacgta
     8581 tatagaaacg aacaatccag aggataacag taccaatatc gatcaggcaa tcagaaacgg
     8641 ctttaccacc attattagct tgagctactt gcaagcagag gaactggaaa atgctgctaa
     8701 agataatcca aagttgaaat ttgccttggt agatggtgag attactggga aaaagaatat
     8761 ctcatcagca acctttaaag atcaagaaag tgcgtattta gcaggactag ctgccgcata
     8821 tacgacaaaa agtgatcaag taggctttat tggtggaatg gaagggcccg tgatccaacg
     8881 tttccaaagc ggatttgaag ctggcgttaa ggcaggggcc acagcactga acaaggaaat
     8941 tactgttaac gtacaatatg ctgcatccta tgattcgcta gataaaggaa aagctattgc
     9001 tgcttctatg tacaaagagg gagtcgatat tctttatcag gcggcaggaa gtactggagc
     9061 tggggtgttc caagaagcaa aagcccgcaa tgaaaacgac tatagcagaa aagtatgggt
     9121 gattggtgta gatcgcgatc aagcggatga aggggattat aaaatggaga acggcgagac
     9181 agggaatttc acgttgactt ctacaaagaa aaatgtcggt gcagaagtca aagaatttgc
     9241 agaaacttct cataacgata aatttcctgg cggcaagcat ttgacctttg gtttgaaaga
     9301 agaaggggta gatctgacaa aaggacaatt atcagaggaa gcctatcagg cagtttcaga
     9361 cgcaaaagag aaaatcatca gtggtgattt cagactgtag acaaagtcca ttttatggat
     9421 gatgtctaca gt
//
LOCUS       DAYYGO010000106         9307 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000106 DAYYGO010000000
VERSION     DAYYGO010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.108"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(38..706)
                     /locus_tag="ACHA5T_002278"
     CDS             complement(38..706)
                     /locus_tag="ACHA5T_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8887831.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            840..1127
                     /locus_tag="ACHA5T_002279"
     CDS             840..1127
                     /locus_tag="ACHA5T_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887832.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1543..3081
                     /locus_tag="ACHA5T_002280"
     CDS             1543..3081
                     /locus_tag="ACHA5T_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFM8887833.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3078..4043
                     /locus_tag="ACHA5T_002281"
     CDS             3078..4043
                     /locus_tag="ACHA5T_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8887834.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4030..5352
                     /locus_tag="ACHA5T_002282"
     CDS             4030..5352
                     /locus_tag="ACHA5T_002282"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8887835.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5415..5519
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5593..6336
                     /locus_tag="ACHA5T_002283"
     CDS             5593..6336
                     /locus_tag="ACHA5T_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8887836.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6351..7343
                     /locus_tag="ACHA5T_002284"
     CDS             6351..7343
                     /locus_tag="ACHA5T_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8887837.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7355..8092
                     /locus_tag="ACHA5T_002285"
     CDS             7355..8092
                     /locus_tag="ACHA5T_002285"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8887838.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8082..8804
                     /locus_tag="ACHA5T_002286"
     CDS             8082..8804
                     /locus_tag="ACHA5T_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFM8887839.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8888..>9307)
                     /locus_tag="ACHA5T_002287"
     CDS             complement(8888..>9307)
                     /locus_tag="ACHA5T_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFM8887840.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3049 a         1604 c         1966 g         2688 t
ORIGIN      
        1 atttctgtaa attaaataaa gtagtgaaaa ataagaatca gtgctctaac tgttcaagag
       61 gatcaagtgg ttcaaatgtt tctgtagtct gttttgcttc caattggggc aaaacagatc
      121 caatttccag atacttcact ttattcaatg tataagatag aatagaacag ctaagtaatg
      181 tatacagaaa atgtgtgaga tccaaataaa gcaaggagac aactaaaata ccaaaagtaa
      241 tcaagaaaag acttttgcca atcttaatat gaaaatattt ttcaatggct tgataaatga
      301 tatctgttcc gcctgtgctt ccgccgaatc gaaagacgag accgcaacca atgccagaaa
      361 gaatacctgt catcaaacta ttgatcagta gactatctac agcaaaatgt atatgaaggc
      421 tctcccataa atgtaaaaag aaaactagcg caaatgttcc gacgatcgtc cggagaaaag
      481 cgtgttttcc aaaaagaaac aatgagataa gcaataaagg taaattaaaa agcagtgaag
      541 tgacagaagg agcgaatcca aaaagattat gcaaaagcaa agcaatacca gtaaccccac
      601 catctgccag cttagatgga atattgattc ctgtcacagc taatccgtag attcctgcgc
      661 ctaaaatgat tccgcacaat tctgtaaata aatttaattt tttcatttct agcctctttt
      721 cttttgaaaa ttttcagcaa cttctttatt atactgactt atactcttac ttcaagagga
      781 aaatgaaaaa aaattatttt tggctgtttc tttttattta tcaaataagg agtggaaaaa
      841 tgcgagcagc agacttattg acaattctta caaaatcacc caaaaaaaca gagtatcaag
      901 gagttttttt ggctcaagaa caaaaactgg tgcctgtgac aatggtccag ataaataaag
      961 caaatcaatt tatcttttta tttgaacacg gcaaagcacc acttgctatg aaagacatct
     1021 ttcttttttt gatgaagaac cgtgagaaag ctgtttatta tcagtcaaaa gaagaactca
     1081 ttcctcttta tggagtgaaa gagatagaaa ataaagtggt gatataagta aaaataagaa
     1141 aacaagacgt ttatgatgtc aaaacaacca ttttttacat ttttggcaaa aaagtgtcat
     1201 gatacagtat aatattcgtt gaacaggcca ataaaggtgt tctcccccac gagatttcta
     1261 ctttattggc tgtttctttt tattaaaata aaagttgtgt gtgtaaaaaa gatatagctg
     1321 attccaagtg acaatttcgt atagattttt atggtattcg tagtgaaaaa acgagaacca
     1381 aaaagttgat ccgactttgt tgcttggatt ctttttttgt cagcatattt agaggtattt
     1441 ttttcttttt tttgttcgtg ctgtaaggtt aaggcaagac aagttagttg ggaaaaagga
     1501 aagaaatgtt tatacttaaa gaataacaga aaggaaggtc gcatgaataa ttttatacaa
     1561 acattaagtg aaagaaaagg cgagttgctg gaagctgcat ttcaacatct gtccatttct
     1621 ctcatttctt tgctgattgc agcactgatt gctattcctt tagcgatttg ggcagccaat
     1681 cataaaaaaa tggcagaagt cttattgcag atcacgggtg tgctccaaac gattccttca
     1741 cttgccttat taggtttact tatcccattt gtagggatcg gaactgttcc agcattgatt
     1801 gcacttgtta tttacgcttt gcttccgatt ttccaaaaca cgtatattgg tctagctgaa
     1861 attgacccgt ctattgaaga agcagcggta gcatttggta tgtccagaat gagacgcttg
     1921 ctgaaagtag agcttcccat cgcattacct gtcattatct caggtatccg aactgcctta
     1981 gttttaatca tcggtacggc tactttggca gcattgatcg gagcaggtgg attagggaca
     2041 tttattttgt taggtattga ccgaaataca cctgttttga cattgattgg tgcgatcagt
     2101 tctgccttgc ttgcaattat attcagcgga ctgattcgct ggttgcagca taagaaacct
     2161 cgatattcca tcattacttt agcagcagtg gtgtgtttat ttggcggcgt ttctttatat
     2221 cagtctgcct tgtttaaaaa tgaaaccatc acgattgctg gaaaattagg agcggaacct
     2281 gaaatcctga tccaaatgta taaagaattg attgaagaca acacaaaaac agaagttgaa
     2341 ttaaaaccga attttggcaa gacgagtttt ttgttcaatg cactggatca aggtcaaatc
     2401 gatatctatc ctgagttcac aggtactgtc ctagaaagct tggtgaaggt acctgaagca
     2461 atgaaaagtg ctgatttgac aaaagaaaaa acatatgacg aagcagaaga attattgaat
     2521 aagcaattcg atatgacatt attaaaacca atggcttacc aaaatacgta tgccttggca
     2581 gtcaagagat cttttgcgaa cgaacatcaa cttaaaaaga tttccgattt ggcagctatc
     2641 tctgatcaga tcaaagcggg attcacactg gaatttattg atcgttcaga tggatataaa
     2701 ggaatccaag aagcatatgg cttgaatttt tcaagtgtac aaagcatgga gccaagtttg
     2761 cgttaccaag caatcaataa tggagatgtc aatattgtgg atgcctattc cacagatagc
     2821 cagctcaagg agtacgatct agtgactcta gaagatgatc gagggttatt cccagcttat
     2881 caaggcgcac cgctgatgaa aaaagatttt gctaaagaac atcctgaaat cgttaaagca
     2941 ctgaataaat taagcggaaa aataacagag gatcaaatga ttgatatgaa ttaccaagtc
     3001 aatgtcgaaa agaaaaagcc gacagaagta gcacgaatct tcctgaaaaa tgaaggatta
     3061 gtaaaggagg aagatcagtg actaaattaa tcgagttcaa acatgtgcaa aaaaagtatg
     3121 atggaaagta tgtcatcgat gacctaaatc tggcaatcaa aaaaggagaa atctttgttt
     3181 tggttggtcc ttccggtagt ggaaaaacaa ctactttgaa aatgatcaat ggattatcta
     3241 aaccatcagc aggagacatc tacttcaaag gaaaatcgtt aaatgaatat aatctgcaga
     3301 aaatgcggtg gaatatggga tatgtgctgc agcaaatcgc tttgttcccg accatgacag
     3361 tcaagcaaaa tattgaagtc atccctgaaa tgttaggttg ggaaaagcag aaaagagcag
     3421 accgtgtaga tgaacttctt caaaaagttg gcttatctcc tgatatttat cgagatcgta
     3481 tgcctagaga gttgtctgga ggagaacagc aacggatcgg gatcatccga gctattgctg
     3541 caagcccaga tgtgattttg atggacgagc catttagcgc attagatccg atttcaagaa
     3601 actcattaca agaattggtt ttgtccttac acgaagaatt gggcacgacc atcgttttcg
     3661 ttacgcacaa tatggaagaa gcaatcaagc taggtgatcg tattgcattt atgaaggatg
     3721 gagaaattat ccaatgtgac acaccagagc agttattgat gaatcctaaa aacgactacg
     3781 ttcggcattt ctttgatgaa ccgaaacaga cgaaagagtg gcgagtagaa gatttagtcg
     3841 tcaatggata tttcttaaat gaaataccag aaaatgctcg tcaacaagtt tgctttgata
     3901 cgcctatgaa agaagtatat tcgcttcttt cggtgtatcc aagcattgtg atagtagaaa
     3961 agcaaagaag tattgggtcg ctgacaagta aagaaatctt tgctttttta agtcgagagg
     4021 aggaaggaaa tgaaaacata tgatgcaatc atcatcggtt caggtgtgag cggcttaagt
     4081 gctgcttatg gattgaaaga agcaggtaaa acagtactag tggtcgaaga agatctatgg
     4141 ggcgggactt gtcctaaccg tggatgtgat cctaaaaaag tcttgttaag tgcagtagag
     4201 gcacgtaatc gggtaaaaca attgagtgga aaaggtttta acgagatccc aactgccaac
     4261 tgggaagaac ttcaaaaatt caaacgaaca tttacggatc cagtcccaga aagccgaaaa
     4321 aaacaattag cagaagctga aatcgatcat ctttcaggaa ccgctcgttt tttggatgat
     4381 tcatctattg aagtcaacga agaagtcttc catgcggatt acttagtttt ggcaaccggg
     4441 caaagaccta cgatcttacc agtggaggga aaggaatact tgaaaactag tgcagacttt
     4501 ttatcattgc ctgtactgcc taaagaaatc atttttatcg gtggtggtta tattgctttt
     4561 gagctagcta cgattgccaa tgctgcaggg agcaaagtaa ccattgtcca tcataatcaa
     4621 cggccattaa aagaatttga ggcgtctttg gtagaggaag ccgtccatca aatggaagca
     4681 tctggcattc agtttgcttt tggtgttgaa acccaaaaga tcatttcaga aggtactcgc
     4741 tatcgtttag tcggaaaaga gacggaatta gtagcagata tgattttttg tgcgactggg
     4801 agacagccga atacagaatc tttagcactt gagcaagcga atatcgtctt tgataaacac
     4861 ggaattgcag tcaatgatta tctgcaaacg agcaatccaa aaatttttgc gtgtggagat
     4921 attgtttcaa ggaaaacccc aaaattgaca ccagtcgcca cttttgaagg aaattatgta
     4981 gctaagcgga taacggatgc aacaagcgaa cctatcaaat atccgattat acctacgatc
     5041 gtctacgcca gtcctaaact tgctgaagta ggagttacaa aaagtcacgc ctcttcgtca
     5101 gatcaagtgg tagagatgga tcttaccagt tggttcacct atcatcgtgt caatgaacca
     5161 gttgctaagg ctgaattgac ctttgaccag caaaattatt tgattggtgc tgcagtgatt
     5221 agcgagcaag cagacgaatt gatcgatgat ttgacactcg tcatcaatca aaaattgact
     5281 aaaaaagagt tggatagcta tatcatgggg tatccgacat tggcaagtga tctctcctat
     5341 ttattgaaat aatcagcatg ataactaaag cgcttgttgc tttttccttc ttgtgctaca
     5401 ctttaaaaaa tgaatacctg tttctgggga tgtcgtagac gacatgagag taagttgtga
     5461 acttagaccc attgaacctg tcagttaata ctggtgaagg gagaaacata gcagacgctt
     5521 gaagcgggtt actatgtcgt gcccatttca agcgtttttt gttattcata ttagaagaaa
     5581 ggaggtaaca aaatgaagaa aaatctttat ccagcagtca gtatggcatt cattttaatt
     5641 atttggcaga ttgttgtcat agtttggaaa atacctgcct ttattttacc cggacctgct
     5701 gctgtatttt ctgcattagc ggcagatgca caagtattac tttcccattc tgtcacgaca
     5761 ttaaatgaag cagtgcttgg tttggtaata gctagcgttc tttcttttat tactgccttg
     5821 gcaatggatt attggaagtt tttagaaatg tctttttatc cgttactagt gatttcacaa
     5881 atgttgccaa tcatggtgtt agggccatta cttaccctat ggtttggttt cggtatgctt
     5941 ccaaaagtga tccttgttgt cttgataagc tattttccga tcgtagtttc tttttcggat
     6001 acattgagga aagtatcaaa agagcaaatc atctttttga aaacgatggg ggcagataca
     6061 gggaagattt atcgaatata taaaatacca gcgggtttat ccggcttctt ttccggttta
     6121 aaagtggctg ctacttattg tgtcagcgga gcagtcgttg gagaatggct aagcgctcaa
     6181 gcaggcttag ggtattacat gatacgcgta aaaaacagct accagatcga taaagttttt
     6241 gctgcgatca tttgtgtcat tctattgagt ttattgctga atggcgcttg ttcattattg
     6301 aaaaaagggt attatcgtat gctttatatt ttttaagaga aggggatcaa atgaaaaaat
     6361 tatggctttt attaccgcta ctgttgcttt tatccgcttg tggcactgca aaagaaacaa
     6421 gtagcaaaca agaaataaag gatttgaaaa aagtgacact tgttttggat tatgtaccga
     6481 atactaatca tactggcatc tacctagcaa aagaaaaagg ctattataaa gaggctggtt
     6541 taaatgtcca aatcatcgaa ccaggagata attcgacaag tattggtcta gtaggtgcgg
     6601 acaaagcaca atttggtgta agttatcaag aagatgtcac ttatgctcac gcagatggac
     6661 agaatatacc agtcaaagca attgctacag tgatcaaaca taatacttct ggattcgcca
     6721 ccctatcaga cagtaacatc cattcaccaa aagattttga aggaaaaaca tatgcaggtt
     6781 ggcaaagtcc aagtgaagaa gctgtcttaa aagctgtgat ggaaaaagac ggcggggact
     6841 tttcaaaact gacaatggta ggaagcaatg gagaagggcc agaaagctta ggaaaatcct
     6901 cagatatcca atggtacttt gaaggttggg atatgatcaa agcaaaagaa gcgggtattg
     6961 aactaaacta tatcccattg aaagaattag atgaacggtt agattactat actccggtaa
     7021 ttattacgaa tgatcaactg atcaaaagtg atccagaatt ggtacagtct tttatggatg
     7081 ccacgaaaaa aggctatcag gaagctatta aggatccaaa tgacagtgct aaactgttgc
     7141 aaaaatatgc aaaagaaaat gatcggacgt ttttagaaga atcacaagca ttcttgtcaa
     7201 aaaattatac ggatgatcct gagaattggg gattgatgga agaaaaagtt tggcgcaatt
     7261 atacagcttt tatgcaggaa aacggattga tcaagcaaga tgtaccttcg caagaactat
     7321 tcactaatca gtttataaaa taggagtgat agatatgtca atccaaatca gccacttgaa
     7381 tgtagtcatt gaaaacctga aaatattaga agacatccat ttcactatcc ctaaagatac
     7441 ctttacatcg attgtcgcgc caagcggagc cggtaagtca acattgctga aaactttaac
     7501 aggagtccag cctatgactt ctggaacgat caaagtagac gatcaaaaag taactggtct
     7561 ccatacagca tttagttata tgccgcaaga agatatgtta cttccttggc tgagcgtcta
     7621 tcaaaacgtt acgttatatc aaaaaatcaa tcatctaaca atcaatgaag aacaagtaag
     7681 ggattatctg gatatctttg ggttaaacgg ctatgaacat tttttgccgg aacaattatc
     7741 tggcgggatg aagcagcgaa cggccttgtt acgaacaatc atgaatcctt cttcttactt
     7801 gcttcttgat gaaccatttg gagcgttaga tgctatgacc agagggcaga tgcaagactg
     7861 gatgttgaag ctgcctaaaa aagcaaagcg gactacttta cttgtgacgc atgatatcga
     7921 agaagcaatt tatttatcag acaggattct tgtgctttcc gcccgtccgg cacatgtgat
     7981 tgctgagatt caagtgcctg aaaaacgacg gtcacgtgaa tggctactgc agcaatcaga
     8041 gttgaagcaa acgatttatc agttattggc aggtgaatta gatgttaagt gatgctcatt
     8101 gtcatttaga tgggagtcag agtttagcac tccttcagca ggaacacacg attttgacga
     8161 tcatcaattg tgattcgcca gaagaatgga aggaaaacag acagcttgca gcaagtaaga
     8221 ctcaagcctt aagttacggt atccatcctt ggaaagcaga tagctatacg tttgaacaag
     8281 ttgagccttt tttgaaaaaa gcaaggatca tcggtgaaat agggttagat aatatttgga
     8341 cgaatgtacc gatgactaca caaaaaaaag tatttgagcg acagcttgct tttgcagcca
     8401 taaatgagaa acctgttgta ctgcacacaa aaggatgcga aaaagagatc ttaaattata
     8461 tccaagctta ccctaatcac tatttgatcc attggtattc ttctttggac tatcaacaag
     8521 actatattga tttaggctgt tatttcagca ttggtcttga tttgaaaaag aatccagcag
     8581 tctggcaact agctaaagca gtcccaatcg accacttact tattgaaacg gatggactac
     8641 atgcagcaga gtgggcttta ggggggattt tttcagaaaa agattatgtc tcactgcttg
     8701 aagaacagtt aaagacaatc gctgagatca aagagctatc agttgaagaa gttaaaaaaa
     8761 atacttatca aaacttggaa cgtttcatta gattaggtaa ataaaaaaga gatgtgacac
     8821 aactagtgtc gcacctcttt ttggctttct ttgtataaaa gtaaactgtt tgattaataa
     8881 atgaatctta gtaccaaccg tttgcttccc agaatgcttt cgccgcttcc caagaaccat
     8941 aacgtgaagt aacatattgt tctgctactc tttcttggtt tgcagcagag tagtcaccat
     9001 ttaagtaaga agaatctaat tggtaacgtc cgatgtaacg tccgttcgtt gctgtgtaag
     9061 aaccgcttga ttctttttgt gcgatccatt cttttgcaga gcttgtgctt gctggagtag
     9121 cagcagtttc tgttacagca ggagcaggtt gtgtttcagc aacaggcgct gcttgttttt
     9181 ccgtttgaac cgtttcttgt acaacaggtt gttcagcttg gactggtgct tcttgaacgg
     9241 ctgcttgagg ttcagcagct gcttgtgcag agccttctgt tgggattgtt aattgttgtc
     9301 ctgagta
//
LOCUS       DAYYGO010000107         9276 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000107 DAYYGO010000000
VERSION     DAYYGO010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.109"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..1458
                     /locus_tag="ACHA5T_002288"
     CDS             4..1458
                     /locus_tag="ACHA5T_002288"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFM8887841.1"
                     /translation="MTRFLRNYIDGRWVASRSTEFEEVMNPATEEALANVPLSSKAET
                     AEAVKAADAAFQTWKKVPVPVRGRYLFKLHQLLTDHKSELARLITLENGKSLSESLGE
                     VQRGIENVEHAAGITNLMMGDSLSTAATDIEITNYRYPIGVIGGITPFNFPMMVPFWI
                     FPMALAAGNTVVLKPSEKTPLLMEKIVELVEKAGFPSGVFNVVYGAHDVVNTLLQDPL
                     VKGISFVGSKNVGEYVYREGTKNLKRVQALTGAKNHVVVLKDANIDRAVKDLIGGAFG
                     SAGERCMAASVIVLEEEIADQFVEKFKAAAKAIKIGNGLDEGVFLGPVIRKDAQQRTF
                     DYIQKGVDEGAKLVLDGRENIPEKGYFVGPTIFDNVTMDMKIWQDEIFAPVASIVRVK
                     DLMAAVELTNESEFANGSCLYTNNASAIRYFRENVDAGMLGVNLGVPAPMAWFAFSGW
                     KHSFFGDLHANGKDSVEFYTHRKVVTARYAQSEI"
     gene            1534..2694
                     /locus_tag="ACHA5T_002289"
     CDS             1534..2694
                     /locus_tag="ACHA5T_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HFM8887842.1"
                     /translation="MNIQLKMPANLRIGGGTIQFLMEESEQLGNKPLIISDTVMEQTG
                     HLQKIKELLGKTDDQLAEYTDIQAEPTQENVYDALKLFQKHQCDHIIAFGGGSCLDTA
                     KVVAVLAANQLSLEAIHARNFDQLQAGIPLIAVSTTAGTGSEVTDVAVITNTKTAVKM
                     MMKDLVFMPQISIVDSDFTKTVPPKVTAATGVDALCHALESYVSVKANEVTQLFSLEA
                     MKKILNTLSQAYENSDDTEAREQMSLASTEAGIAFSNASVTLIHGMSRPVGALFHIPH
                     GISNAMLLPVFFEFSKDAIQTELATIARFIYPAAEDLSLEEAADYLIKKIQELCEQLS
                     IPTIRDYGIDQQAFMAALDKMAADALTSGSPQNNKKVPSHSQIKELYVQAYG"
     gene            2807..3814
                     /locus_tag="ACHA5T_002290"
     CDS             2807..3814
                     /locus_tag="ACHA5T_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8887843.1"
                     /translation="MRVKVGVIGTGAIGIEHIDRINQKTQGAYVTAVSDINVKAAKEV
                     AKRVDAKFFETGEALIASEDVDVVVVTSWDPTHEQYVLEAIKHGKYVFCEKPLAMDAA
                     GCRRIVDAEVAYGKKLVQVGFMRRYDRGYIELKEAIESKQYGEALMMHCAHRNPSADE
                     NYTTPMAISNTAIHEIDVLRWLLNEDYESVQMILPKKTSNSHADLHDPQLLIFQTKSG
                     VTIDLEIFVNCRFGYDIKCDVVFETAEVGMTDPAYTRIKSEEKNYTPVSPDWQTRFLD
                     AYDYEFKLWIDSIKNDAVVGPTAWDGYVAAITMMACHESRETKTKVMIQMDERPALYN
                     E"
     gene            3838..4863
                     /gene="iolG"
                     /locus_tag="ACHA5T_002291"
     CDS             3838..4863
                     /gene="iolG"
                     /locus_tag="ACHA5T_002291"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050112 - inositol 2-dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HFM8887844.1"
                     /translation="MNNIRVGIVGLGRLGKVHAQNLATGVSCCELTAACSLVETELAF
                     AQKKLGVKHTFSSYEEMVKSSVIDAVFIVSPSGFHCDQVRLAMENGKHVFTEKPLGLE
                     VEDIKKTQKVIEEHPEQVFMLGFMRRYDESYQYAKKMVEDGEIGEITLIRCYGIDPSE
                     GLESFVKFAMNNDSGGLFLDMAIHDIDLVRWFTGQEVDQVWAIGKNAAYPELDQAGEL
                     ETGAAMMSLSDKTIALLVAGRNAAHGYHVETEIMGTKGMLRVAQAPEKNLVTIMNDQG
                     IIRPCSQNFPERFREAFIREAQEFVSCIQEKRQPAVNAEDGLQSTKIALACQESFDTK
                     SLVQLKK"
     gene            4880..5770
                     /gene="iolE"
                     /locus_tag="ACHA5T_002292"
     CDS             4880..5770
                     /gene="iolE"
                     /locus_tag="ACHA5T_002292"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010777829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050114 - myo-inosose-2 dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HFM8887845.1"
                     /translation="MTKQIQLAIAPIAWTNDDMPELGSENTFEQCISEMALAGFTGTE
                     IGNKYPKDPEVLEQYLNIRGLKVASAWFSAFLTTKPFEETAEAFKAHRDFLHAMGAKV
                     IVVAEQGHSIQGIMTAPVFDDKPVFTDEEWKKLAEGLEKLGDLAHEKDMEIVYHHHMG
                     TGVQTTEEIDRLMEMTDPDKVKLLFDTGHLVFSGENPIAVYNRHQDRIKHIHFKDIRQ
                     ETAEEVEKEKDSFLNGVKKGVFTVPGDGMIDFTPIWTAIHASDYEGWIVVEAEQDPAK
                     ANPFEYAVKARSYIREVTGL"
     gene            5793..7388
                     /locus_tag="ACHA5T_002293"
     CDS             5793..7388
                     /locus_tag="ACHA5T_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFM8887846.1"
                     /translation="MNLFSIISFLVIVICVWLFAYSRSRSVDTSGSEGFFMGGRSLTA
                     LPIAGTIIMTNLSTEQIVGQNGQSYHAGMEVMAWEVTATIAIVALAVIFLPKYFKYGI
                     NTISDFIEIRYDTVTKRIISILFIVTYMTSFLPVVLYSGSLVFNKIFHIDELLGVQPI
                     VAIIIVAMAIGIVGILYLLIGGLSLSANSDSIYGVGLLACGLLVPILGLMKLGDGSFV
                     GGIEHVVVNTPWLLNSVGSIDSAVVPWPTLFTGMLFNNLYFWCTNQMIVQKALSGKNL
                     AEAQKGAFLVGVFKILGALFLVFPGIVARNIFGDSLMSNPDNAYPFLVSEVLPKMIFG
                     IFAAVIFGAILSSFAGALNATSTLFSLDFYKPLINKKANDKQIARAGKTVTVVVGIIS
                     IIVAPFISFAPAGLYQFVQEFNGLYNMPLLVIILFAFYSKKATAFGAKTTIAVHIVLY
                     ALSKMLFQNIHFLYVLTALFVLDIIILMVVSKLKPDGEFVLNSFDTKVDIEPWKYTKV
                     TAIALVVIVILTYVAFSPIGFAG"
     gene            7404..7718
                     /locus_tag="ACHA5T_002294"
     CDS             7404..7718
                     /locus_tag="ACHA5T_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFM8887847.1"
                     /translation="MHKDLLIICETGISASLLVSKMLETIRAKNLSYEIDYATIRNVE
                     EKLNYKDYDVLLLTPQVARSEEKVKEIIKKEKCTAQIVYIKQEDFRYMNIENIIASIE
                     ED"
     gene            8179..8904
                     /locus_tag="ACHA5T_002295"
     CDS             8179..8904
                     /locus_tag="ACHA5T_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM8887848.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGGTA"
     gene            9037..>9276
                     /locus_tag="ACHA5T_002296"
     CDS             9037..>9276
                     /locus_tag="ACHA5T_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFM8887849.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASII"
BASE COUNT         2827 a         1670 c         2164 g         2615 t
ORIGIN      
        1 taaatgacaa gatttttaag aaattatatt gatggtcgtt gggtcgctag tcgctcaaca
       61 gagtttgaag aagtaatgaa tccggctaca gaagaagcgc tggcgaatgt accgttgtct
      121 agcaaagcag agacagccga agcagttaaa gctgccgatg ctgcgtttca aacttggaaa
      181 aaagttcctg ttcctgttcg cggacgttat ctgttcaagc tgcatcagct tttaacagat
      241 cataaaagtg aattggcgcg tttgatcacg ctggaaaatg ggaaatcatt gtccgagtca
      301 ctgggagaag ttcaacgagg aatcgaaaat gtggagcacg cagcgggaat caccaattta
      361 atgatgggag attctttatc aacagcggcg actgatattg agatcacgaa ttatcgttac
      421 ccaatcggag taatcggagg gatcacaccg tttaattttc caatgatggt gcccttctgg
      481 atcttcccga tggctctggc ggcaggaaat acggtggtgc tgaagccttc agaaaaaaca
      541 ccattgttga tggagaaaat tgttgagctg gttgaaaaag caggctttcc tagcggtgtt
      601 tttaatgttg tctatggtgc ccacgatgtt gtcaacacat tgctgcaaga tccactggta
      661 aaaggcatca gctttgtagg atcaaaaaat gtcggtgaat acgtttaccg tgaaggaaca
      721 aaaaatctaa aacgggtaca agcgttgact ggagcgaaaa atcatgttgt tgtgctgaaa
      781 gatgccaata ttgatcgggc agtcaaggac cttattggcg gagcttttgg ttctgctggt
      841 gaacgctgca tggcagcctc cgtcattgtt ttggaagagg aaatcgcgga tcagtttgtt
      901 gaaaaattta aggctgcggc aaaagcgatc aagatcggca atggcttgga cgaaggggtc
      961 tttttaggac cagtgattcg aaaagatgca cagcagcgga cctttgacta tatccaaaaa
     1021 ggggtcgatg aaggagcgaa actggtacta gatggtcgcg aaaacattcc agaaaaaggc
     1081 tattttgttg gaccaacgat ttttgataat gtcacgatgg atatgaagat ctggcaggat
     1141 gagatttttg ccccggtcgc atcaatcgtg cgggtaaaag atttgatggc agccgttgag
     1201 ctgacgaatg aatctgaatt tgcgaatggt tcgtgtctgt ataccaacaa cgcatcagcg
     1261 attcgctact tcagagaaaa tgttgatgct gggatgctgg gggtcaatct tggggtgcct
     1321 gcaccgatgg cttggtttgc cttttcaggc tggaagcact ctttctttgg tgatttgcat
     1381 gccaatggca aagacagtgt tgagttttat acccatcgga aagttgttac agcacgttat
     1441 gcgcaaagtg agatttaaag atttgcttcg aagctctgtt aaagagcttc gaggattctg
     1501 ttataggcta taagccagag agaggttttt tctgtgaata ttcaactgaa aatgcctgcc
     1561 aatttaagaa ttggcggcgg aacaatccaa tttcttatgg aagaatcaga acaactaggc
     1621 aacaagccgt taatcattag tgatacggtt atggaacaga ccgggcatct tcaaaagatc
     1681 aaggagctgt tgggaaaaac agatgatcaa ttagcggagt atacagacat tcaagccgaa
     1741 ccgacgcaag aaaatgttta tgatgcgctg aagctttttc agaagcatca atgtgatcat
     1801 atcattgctt ttggtggcgg cagctgtctg gatacagcca aggtggtagc cgttttggct
     1861 gctaatcaat taagtctcga agcgattcat gcaagaaatt ttgatcagct gcaagcgggc
     1921 attccgctga ttgcagtgtc aacaactgct ggaactggtt cggaagtaac cgatgtggca
     1981 gttattacca atacaaaaac agcagtcaaa atgatgatga aggatctcgt tttcatgcca
     2041 cagatctcaa tcgttgactc tgattttacg aaaactgtac caccaaaagt aaccgcggca
     2101 acgggagtcg atgcgctatg tcacgctttg gaatcttatg tttcagtcaa agccaatgaa
     2161 gtaacgcaac tattttcact ggaagcgatg aagaaaattt tgaacacgct ttctcaggca
     2221 tatgaaaata gcgacgacac tgaagccaga gaacaaatga gcttagcttc aacggaggcg
     2281 gggattgcct tttcgaatgc gtcagtcaca ttgattcatg gaatgagtcg accggtgggg
     2341 gcactttttc acattcctca tgggatatca aatgcgatgt tattgccggt cttttttgaa
     2401 ttctcaaagg atgctattca gactgagtta gcgacgatcg ccagatttat ttatccggca
     2461 gccgaagatt tatcgctgga agaggctgcg gattatctga ttaaaaaaat ccaagagctc
     2521 tgtgagcagc tgtcgatccc aacgattcga gattatggta ttgatcagca agcgtttatg
     2581 gcagccttag acaaaatggc cgcggatgcg ttgaccagcg gaagtccgca aaataataaa
     2641 aaggtgccta gtcatagtca aattaaggaa ttatacgtac aagcttatgg gtagtaaaag
     2701 gcggagcaaa gagagggctc taccttttaa gaataaaagc tacacaggaa taattttgta
     2761 agcgcatact atgtgtgctt aaacaacaga acagggggat aagtaattga gagtaaaagt
     2821 aggcgttatc ggaacaggtg caatcggcat cgagcatatc gacaggatca atcagaaaac
     2881 acaaggggct tatgtgacag cagtctcaga tatcaatgta aaagctgcta aagaggttgc
     2941 caaacgtgtc gatgctaaat tctttgaaac cggagaagca ttgatcgctt ctgaagatgt
     3001 ggatgtcgtt gttgtgactt cttgggaccc gacccatgag cagtatgtgt tagaagcaat
     3061 caagcatggg aaatatgttt tctgcgaaaa gccattagcg atggatgcag caggctgccg
     3121 ccggatcgtt gatgcggaag ttgcttatgg gaagaaattg gttcaggttg gcttcatgcg
     3181 ccgctatgac cgcggctaca ttgaactgaa agaagcaatc gaatcgaagc aatacggaga
     3241 agcattaatg atgcattgtg cccatagaaa tcctagcgca gatgaaaact acacaacacc
     3301 gatggcgatc agcaacaccg cgattcatga gattgatgtg cttcgctggt tgctaaatga
     3361 agattatgaa tctgttcaaa tgatcttgcc gaaaaaaacc agcaactccc atgccgattt
     3421 gcatgatccg cagctgctga tctttcaaac aaaatccggt gtaacgattg atttggaaat
     3481 ctttgtgaat tgccgctttg gctacgatat taaatgtgat gtggttttcg aaacggcaga
     3541 agtaggaatg acggacccag cctatacacg aattaaatcg gaagaaaaaa attacacacc
     3601 tgtttcacca gattggcaga cccgcttttt ggatgcctat gattatgaat tcaagctgtg
     3661 gatcgattcg atcaaaaatg atgcggttgt cggtcctacc gcttgggatg ggtatgtagc
     3721 agcgatcacc atgatggctt gtcatgagtc tcgtgaaaca aaaacaaaag taatgattca
     3781 aatggatgaa cgaccagcac tatacaatga gtaattctca gaaaacggag gtatttcatg
     3841 aacaacatta gagtaggcat cgtcggttta ggaagattag gaaaagtaca tgcacaaaat
     3901 ttagcaacag gtgtcagttg ttgtgagctt acggctgcgt gcagtttagt tgaaacagaa
     3961 ttagcattcg cacagaagaa attaggcgtc aagcatactt tttccagcta tgaagaaatg
     4021 gtcaaaagtt ccgtgatcga tgcagtcttt attgtttcac catcaggctt tcactgtgac
     4081 caagtccgct tggcgatgga aaatggcaag catgtcttta ctgaaaaacc attaggcctt
     4141 gaagttgaag atattaaaaa gacccaaaaa gtcatcgaag agcatcctga acaggtcttc
     4201 atgttaggct tcatgcgccg ctatgatgag tcgtatcagt atgcgaagaa aatggtagaa
     4261 gatggcgaaa tcggcgaaat cacactgatt cgttgttatg ggatcgatcc gagcgaaggc
     4321 ttggaaagct ttgtcaaatt tgccatgaat aacgacagcg gcggcttgtt cttagatatg
     4381 gcgatccatg atattgattt ggttcgctgg tttaccggcc aagaagtcga tcaagtgtgg
     4441 gcgattggga aaaatgcggc gtatccagaa ttggatcagg ctggtgagct tgaaactgga
     4501 gccgcaatga tgagcctgag cgataaaaca atagctttac tagttgccgg cagaaacgca
     4561 gcccatggct atcatgtcga aacagagatc atgggaacca aaggaatgct gcgggtagcg
     4621 caagcgcctg agaaaaatct tgtgacgatc atgaacgatc aagggatcat ccgtccatgt
     4681 tcacaaaact tcccagaacg attcagagaa gccttcattc gtgaagccca agaatttgtc
     4741 tcatgtattc aagaaaaacg tcaaccagcg gttaatgcag aagatggatt gcaatcaact
     4801 aagatcgcgt tggcttgtca agaatcattc gatacgaaga gtttagtaca actaaaaaaa
     4861 taagcaggag tgtgagaata tgaccaaaca aattcaatta gccatcgcac caatcgcgtg
     4921 gaccaatgat gatatgcccg agcttggtag tgaaaatacc tttgaacaat gtatcagtga
     4981 aatggccctt gctgggttca ccggaacaga gattggcaac aaatatccaa aagatccaga
     5041 ggtgttggaa caatatttga atattcgcgg cttaaaggtt gccagtgcat ggttcagtgc
     5101 gtttctgaca actaaacctt ttgaagaaac tgccgaagcc ttcaaagcac atcgggattt
     5161 cttacatgcg atgggcgcta aagtcatcgt agtagctgag cagggtcata gtatccaagg
     5221 gattatgaca gcgccagtct ttgatgataa accagttttc actgatgaag agtggaaaaa
     5281 actggcggaa ggattagaaa aattagggga tttggcccac gaaaaggata tggagatcgt
     5341 ttaccatcat cacatgggta ccggcgttca aacgacggaa gaaatcgatc gtttaatgga
     5401 aatgactgat ccagacaaag ttaaattatt gttcgatacg ggacatttgg tcttttctgg
     5461 tgaaaatcca atcgcggttt acaaccgtca tcaagatcgt atcaaacaca tccatttcaa
     5521 agatattcgt caagaaacgg ctgaagaagt agaaaaggaa aaagacagct tcttaaacgg
     5581 cgtgaagaaa ggcgtcttca cggttcctgg tgatgggatg attgatttca ctccaatctg
     5641 gacagcaatc catgccagcg actatgaagg ctggatcgta gttgaagcag aacaggaccc
     5701 ggctaaagcc aatccatttg aatatgcagt caaagccaga agctacattc gagaggtcac
     5761 cggtctttaa tctattgaaa aggagctaga cgatgaatct tttttctata atcagtttcc
     5821 tagtgattgt catctgtgta tggttatttg cctattctag atccagaagt gtcgatactt
     5881 ctggatcaga agggttcttc atgggagggc ggagcttaac tgctttgcca atcgccggca
     5941 cgatcatcat gacgaacctg tccaccgaac aaatagtcgg acaaaatggt caaagttatc
     6001 atgcagggat ggaagtaatg gcctgggaag taactgctac gattgcgatc gtagctttgg
     6061 ctgtgatatt cttgccaaaa tattttaaat acggcatcaa tacaatctca gatttcatcg
     6121 agattcgtta tgatacggtt accaaacgaa ttatttcgat cttatttatt gtgacctata
     6181 tgacttcttt cttacccgtg gttctttatt ctggatcgct ggtgttcaat aagatcttcc
     6241 atattgatga gctgctgggc gtccagccga tcgtcgcgat cataattgta gcgatggcaa
     6301 ttggtattgt cggcatcctc tatctgctga tcggcggctt gtcgttaagt gccaatagtg
     6361 actccattta tggagtcggt ctgctggctt gtggtctgct ggtcccaatt ttaggcttaa
     6421 tgaagcttgg tgacggcagc tttgttggcg ggatcgaaca cgttgtggta aacacgccat
     6481 ggctgcttaa ctctgtggga tcgatcgact cggctgttgt tccatggccg acactattta
     6541 cagggatgct gtttaacaat ctgtatttct ggtgtaccaa tcaaatgatc gtccaaaaag
     6601 cattgtccgg caagaattta gctgaggcac aaaagggcgc tttcctagtc ggcgtcttca
     6661 aaatcttagg tgcgttattc cttgtctttc cagggatcgt cgctcgcaat atttttggcg
     6721 atagtttaat gtcaaatcca gataacgcgt atcctttttt agtcagtgaa gtattgccta
     6781 aaatgatctt tggtattttc gccgctgtta tctttggcgc gatcttatca tcatttgcgg
     6841 gagctttgaa cgcgacatcg accttgtttt cactggattt ttataaaccg cttatcaata
     6901 agaaagcaaa tgataagcaa atcgctcgtg ctggtaaaac agtaacggtc gttgtgggga
     6961 ttatctccat tatcgtagcg ccattcattt cttttgcacc ggctggattg tatcagttcg
     7021 ttcaagaatt caatggacta tacaacatgc cgctgctcgt gatcattctc tttgcctttt
     7081 attcaaaaaa agcaactgct tttggggcaa aaacgacgat tgccgtgcat atcgtcttgt
     7141 atgcactatc aaaaatgctt ttccaaaata tccatttcct ttatgttttg acggcattat
     7201 tcgttctgga cattatcatt ttgatggtgg tttccaaatt gaaacctgat ggagaatttg
     7261 tgttgaactc attcgataca aaggtcgata tcgagccttg gaaatacacc aaggtgactg
     7321 ctatagcatt ggtagtcatt gttattttaa cttatgtggc attttcacca attggctttg
     7381 caggctaaaa acaggagggt attatgcata aggatctatt gattatttgc gagaccggaa
     7441 tcagtgcgtc gctgctcgta tcaaaaatgc ttgaaacgat cagagccaaa aatctatcgt
     7501 atgagataga ttatgcaacg atccgaaacg ttgaagaaaa actgaattat aaagactatg
     7561 acgttttact tttaacgccg caggtcgcac gttctgagga aaaagtaaaa gaaatcatca
     7621 aaaaggaaaa atgtaccgct cagatcgttt atatcaaaca agaagatttt cgttatatga
     7681 atattgaaaa catcattgca agcatcgaag aagattaacg agaatctttg attattgagt
     7741 gtcgaggagg tggtatatga aaagatggca tgttcgcccc gatcacccca atatgaaggg
     7801 cggacttatt agaaataccc aatcttgtat ttattcaata gggtaagcgg acctcaatag
     7861 ccagctcatc cccaaaaagt cgaatggaga cacttaaatg agtgtaccta ttcgactttt
     7921 tgagactcta agtcaaatcg tgttgttctc attaaatcac tgaaaggaaa tgcttcggcg
     7981 tttccttttt tctttttgtt tttcttcgtg ttttgaaaat tacttactat ttactatacg
     8041 agtagtttca aataattttg acatcctaat cttcaacaac agaaaaaaat ctaaattata
     8101 aaatttaggg ttgactaaaa tataattttt gtataccata tgatcgtgat aatcattatc
     8161 atttagaagg agaaagttat gttagaagtg aaaaagctta atgtttgtta taacgatttt
     8221 ttagcattga acgagatttc tttaaaaata caagaaggtg ctttaacagg tattattggt
     8281 ccaaatggtg ctggaaaatc tacattgctt aaagcaatgt taaatattat ccctcatcag
     8341 ggagaggtct ccattaataa tgaagatatc aataaaaaac tggtgaagat tgcatatgta
     8401 gagcaaaaag ctgatattga ttttactttt ccaataaaag taaaagaatg tgtatctatg
     8461 ggaacatatg ccggaatgaa agtttttcaa agaattaaaa atgcagaatg gcaaagagtt
     8521 tcaaaggctt tggaaaaggt cgatatggga aaatattcta atcatcaaat aggagaatta
     8581 tctggaggac aatttcaacg tgtactttta gcaagatgtc ttgctcaaaa tgcggatttc
     8641 atatttttag atgaaccatt tgtagggata gacttagtta gtgaacgaat aattatggat
     8701 acgttaaaag aattgaaaca tcagggaaaa acagtgctaa ttgtgcatca tgatttaagc
     8761 aaggtaaaga agtattttga taatattatt cttttgaatc gaaatctaat tgctcacgga
     8821 agtgtagaat ctgtatttaa tgaagagaat ttaaaaaaag cctatggaga tactatattt
     8881 attggagagg ggggaacggc ttagcctctc aatccaataa atatattatc tcaaaagaaa
     8941 tgagttaata ccaaattaaa ataacgatgg ttcatagggt tatccaatca aaaccggaag
     9001 gttaaaaccc taactctaat atacaagggg aataatatgc ttcaaaattt tattaatggt
     9061 ctgtatgact ttcacttttt acagaatgct ctaattacat ctgtagttat agggattgtt
     9121 tctggaacag taggttgttt tattatttta agaggaatgt ctcttatggg tgatgctatt
     9181 tctcatgctg ttttgccagg agtagcaatt tcttatattt taggtattaa tttttttatt
     9241 ggtgcgattg tatttggtct attagcttct atcatt
//
LOCUS       DAYYGO010000108         8961 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000108 DAYYGO010000000
VERSION     DAYYGO010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.110"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..74
                     /locus_tag="ACHA5T_002297"
     tRNA            2..74
                     /locus_tag="ACHA5T_002297"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            78..150
                     /locus_tag="ACHA5T_002298"
     tRNA            78..150
                     /locus_tag="ACHA5T_002298"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:111..113,aa:Phe,seq:gaa)
     gene            155..225
                     /locus_tag="ACHA5T_002299"
     tRNA            155..225
                     /locus_tag="ACHA5T_002299"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:187..189,aa:Gly,seq:tcc)
     gene            238..311
                     /locus_tag="ACHA5T_002300"
     tRNA            238..311
                     /locus_tag="ACHA5T_002300"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:272..274,aa:Ile,seq:gat)
     gene            323..394
                     /locus_tag="ACHA5T_002301"
     tRNA            323..394
                     /locus_tag="ACHA5T_002301"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:356..358,aa:Glu,seq:ttc)
     regulatory      583..662
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            660..1853
                     /gene="metK"
                     /locus_tag="ACHA5T_002302"
     CDS             660..1853
                     /gene="metK"
                     /locus_tag="ACHA5T_002302"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFM8887850.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1983..3455
                     /locus_tag="ACHA5T_002303"
     CDS             1983..3455
                     /locus_tag="ACHA5T_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFM8887851.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3538..4326
                     /locus_tag="ACHA5T_002304"
     CDS             3538..4326
                     /locus_tag="ACHA5T_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8887852.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(4393..5061)
                     /locus_tag="ACHA5T_002305"
     CDS             complement(4393..5061)
                     /locus_tag="ACHA5T_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFM8887853.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            5176..6888
                     /locus_tag="ACHA5T_002306"
     CDS             5176..6888
                     /locus_tag="ACHA5T_002306"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8887854.1"
                     /translation="MKLILRHAKKYKVSVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6891..8663
                     /locus_tag="ACHA5T_002307"
     CDS             6891..8663
                     /locus_tag="ACHA5T_002307"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8887855.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(8745..>8961)
                     /locus_tag="ACHA5T_002308"
     CDS             complement(8745..>8961)
                     /locus_tag="ACHA5T_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8887856.1"
                     /translation="AVSKELNLDKKVSSHMLRHSHISLLTELGIPLKAIMDRVGHEDS
                     KTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT         2685 a         1515 c         1984 g         2777 t
ORIGIN      
        1 tggttccgtg gtgtaggggt taacatgcct gcctgtcacg caggagatcg cgggttcgat
       61 tcccgtcgga accgtttggc tcggtagctc agttggtaga gcaatggatt gaagctccat
      121 gtgtcggcag ttcgattctg tcccgcgcca ttatgcgggt gtagtttagt ggtaaaacca
      181 cagccttcca agctgttgtc gcgagttcga ttctcgtcac ccgcttttag tttttatggg
      241 cctatagctc agctggttag agcgcacgcc tgataagcgt gaggtcgatg gttcgagtcc
      301 atttaggccc atttatttta ttggcccgtt ggtcaagcgg ttaagacacc gccctttcac
      361 ggcggtaaca cgggttcgaa tcccgtacgg gtcatggaag atactttttg agtatctttt
      421 tctttttatc tgaaagaatg attttaaaaa gcttagcaga gttatcttta tggatgattg
      481 tgctaagctt ttttggacag ttctaatgta aaatattttt atatttttta agcaaaaaaa
      541 tgaaaaaata cgagcagaaa actgttttat ctgctatact tggttcgttg caagttcccg
      601 aaaggatccg caggacggag atgccttgta accgaattta actaggggga atcattataa
      661 tggtagaaag acacttattt acatcagaat ctgtttctga aggacatcca gataaaattg
      721 ctgaccaaat cagtgatgca atcttggatg caattttaaa acaagatcca acagcacgag
      781 tagcgtgtga aacgtctgta acaactggtc ttgttttagt atttggagaa atatctacga
      841 cggcatatgt agatattcaa aaagtcgtac gggaaacaat aaaagaaatt ggttatacac
      901 gtgcaaaatt cggatttgat ggagatacag cagctgtatt ggttgcgatt gatgaacaat
      961 ctcctgatat tgctcaagga gttgatgaag cacttgagat ccgggatgaa gataaaaaag
     1021 acgtattaga tgagattggt gctggtgacc aaggtttaat gtttgggttt gctgtagatg
     1081 aaacaccaga attgatgcct ttaccaatcg ctttgagtca tcgtctggta cgacgcttgg
     1141 cagatttgcg taaatcaaat gaattaacgt atttacgtcc agatgcaaaa tctcaagtaa
     1201 cggttgaata tgatgatcaa ggacaaccgg aacgcgtaga tacaatcgtt atttcaacac
     1261 agcatgatga tgcagtagat aatgaaacaa ttcgtcatga tgtcatcgaa aaagtagtga
     1321 aggaagttat tccagctgaa ttattagatg atcaaacgaa atattatatc aatccgactg
     1381 gccgatttgt cattggtggt cctcaagggg atgccggatt aacaggaaga aaaatcattg
     1441 ttgatacgta tggcggttat gctcgtcatg gtggcggtgc tttttctggt aaagatgcga
     1501 caaaagttga ccgttctgcc agctatgctg cacgttatat tgctaaaaac attgttgcag
     1561 cagggcttgc tcgcaaagca gaagtacagc tagcttatgc gattggtgtt gctcaacctg
     1621 tttcgatctc aatcaatact ttcggtactg gaacggttcc ggaagaagaa ctgattgctg
     1681 cagtgagaga aaacttcgat cttcggccag caggaatcat cgagatgctt gatttgcgtc
     1741 gtccgattta caaacaaaca gctgcttacg gtcattttgg tcgtacagat gtagatttgc
     1801 cttgggaaca tacggacaaa gtagatgcgt tgaaagcaag tttagcaaaa taagttatat
     1861 tcagctggaa cgatcgccaa gtggtggttg ttccagcttt ttttatggca gttaacctct
     1921 tgaaaaagat agtaattatt tatctatttt tagatcctgt cataatgaaa gggaggaata
     1981 agatgaaaaa agaaacaaat gttactttgg tcacagtgag tattttcatt gcaacgttca
     2041 tgacggctat tgaaggtaca atcgtaacaa ccgctatgcc aacgattgtc ggttcattac
     2101 atggaatgga aatcatgaac tgggtatttt cgatttattt gctgacaaac gctatgctta
     2161 ctccgattta cggaaaactt gcggataaag tgggacggaa accaatcttt atttttggga
     2221 cattattgtt tattatcggt tcgttatttt gcggtttatc tcaaagcatg attgggttga
     2281 ttgctgctcg tgcgattcaa gggatgggtg ctggggcaat gatgccagta gctttgacga
     2341 tcatcggcga tctttattct attgaaaagc gagcaaaagt tttaggtttg aatagttcag
     2401 cctgggggat tgccagtgtt tttggaccgc ttgcgggagg attcatcgtt gaaacgatca
     2461 gctggcactg gatctttttt atcaatgtgc cgatcggaat tctattaatt attttgattt
     2521 ggctatattt aaatgaacca aaaatcatac atgagtctaa accaatggat atttttggta
     2581 gttttagttt gatggccatg cttgtctcat tgctgttagg gttccaatta ttaggagacg
     2641 aaggaatgag tccgcttgtt tctgctttgc tgctaggtag tatcgtaatg ttaggtattt
     2701 ttgtttttgc cgaaaaaaga gctaaagatc ctgtcatcat gatgcattta ttcaaaaatc
     2761 cactatttgt tgtggtcaac ttggctgcag cattggctag tggttttttg atgggaatcg
     2821 atgtttatat ccctatgtgg atgcaaggtg tattagggct gaatgcagca attggtgggc
     2881 tggtattagc accattatca ctagtctgga tggtcggttc tttccttgca agcaagtgga
     2941 tgactcaaat gaaaactcat tgggtcttga gaattggact actaatcgca gctattgggg
     3001 gcgcttggtt gtatcttatg cctttcagaa gtaactattt atggtttttt gctatatcaa
     3061 cggtcatcgg tataggctta ggtgtgacca tgactacctc gactgtcagt gcacaaaata
     3121 gtgtatctcg tgaccagctt ggtgtagcaa cttcttttaa tacgttagtt cgaacaattg
     3181 gtcaaacagt catggtttct atttttggtt tattgataaa tggggtaact aaacgagaac
     3241 ttgcagcagc aaatttaaca aatgatgcag atatcatgaa taaactggtg aatccacaaa
     3301 cggctaaata tattgatgcg gatattttgt taccattaag aaaaattctt tatgatgggt
     3361 tacattccgt ttatctagtt ggcttgttac tcgtgatcat tgctttggtc ttaacgcttt
     3421 tcgttcatga aaaaagtggt aaaataaaac agtagtttcg gttaagatat gtacgtagcc
     3481 gaatcattca aatttaaagt tattcaaaaa taacttctta ctaaggaagg cgaaaatatg
     3541 gcatttattc aagcaaatat ttattcgaat gtgttagaaa tggaagtcaa tctaaatgtg
     3601 atactaccgc aaacaacaaa aaaggtgatt ggcaccagtt cagagcaaga gacaacggat
     3661 atacctgttt tatatttact tcatggaatg ggtggcaatc atagtgtatg ggaaagacgt
     3721 acgtcgattg aacgttatgt agcaaactac ggattagctg tcatcatgcc atctactgat
     3781 cttggctggt acacagatac agtttatgac atgaagtatt ggacattcat tgcagaagag
     3841 ctacctgaga tttgtcatga attattcccg caattaacaa gaaaaagaga aaaaacatat
     3901 gccgctggat tatcgatggg cggatatggc gcgttaaaac taggccttgc aaaatcagaa
     3961 aatttcgcag cagtaatctc tctttcaggc gctgtttctg taggaaatcg aatggatgat
     4021 cttttgatgg ttcgtaaagc atctttttgg gaagggatct ttggaccatt ggatcaaatc
     4081 gaaggttcaa aaaatgatcc gcaatactta ttgaaagaac tagtaaaaag cggaaaacca
     4141 gttccaagat tttatatggc ttgtggggaa tctgattttc tttatcaggc aaatcaggaa
     4201 atggtatctg ctatgcagga acaaggcttg gcggtcacct ttgaacaagg acctggcgat
     4261 cataactgga tcttctggga ccaatggatt caacgtgcat tggcgtggct aatggaagaa
     4321 aaataaaaaa agagtcttaa aaagatagct cctatagaga tcacaaacat agtgatctct
     4381 ataggagctt ttttactgct cgttttctct tcttttattt tgttggcata agccttcaat
     4441 ataggtttgt gcactcgtta gaaagagtgc tttttcattc aattctgcat gggactgcat
     4501 actttcttta aatccctcta attgattttc aactacataa ttacgcataa atagaactgt
     4561 ttctttcaaa aattcatttc cgtccataat tttttgtcga agctgctgtt catgctgctg
     4621 atccattaac tgtccaatca ataaattgtg gagagaggca atggcctgat gactttgggt
     4681 gactgaaaat ccttcacgaa caagtaattg gatcatttgc tccaaatgtt ctaatcgact
     4741 cggaaaagcg ggcacagtat tgatttctaa ttctgcccca caagggaaac gtgtataaag
     4801 gtcatagtaa ttttccatat actgcagcaa ctgttcattc caaggcaaag ttggatctgg
     4861 aagcgctaat tcttcttcca ttgtttcagc cattttttgc aagagctgtt gtttgttttt
     4921 gaaataccaa tataaagcag gtgcttgaac atgaattctg tttgcgatgt ttcgcatgga
     4981 aagctgcgaa agagcttgtg tttcagccag gatatcaaag gcggcctgaa tgatgattgc
     5041 ttgagaaagc tttatttcca ttttatcaac ctcgtgtatt gcattatctt tttaaacatg
     5101 gtaaattgtt agtcggtcta attttacagt gttaaattag tcctgtcaat ttaaacaaag
     5161 gggtgtaatc aacgaatgaa attaatctta cgacatgcga aaaaatataa agtatctgtt
     5221 tttatttcct tgctgtccgt agcagtcatg gttgcagcag cattatggca acctaagtta
     5281 ctacagcaag tattggaagc tattattaca gaagataatg atgaaatgaa aacaattggt
     5341 atttacctga tcagtctggc tttgctagga ttagccgcag gtgtaacgaa cacgattttc
     5401 tctgcaaaag tagctcaagg cgtaagtgca gatatccgag aagaagcatt tcgaaagatc
     5461 cagacatttt catttggaaa tatcgaacag ttctctgctg gaaatcttgt tgttcgtcta
     5521 acaaatgata tcacgcaaat ccaaaattta gtgatgattt ctttgcaatc actttttcga
     5581 attccattct tatttattgg tgccttcatt ttagctatga tcacgatgcc gcaattatgg
     5641 tggatcatcg tagcgcttat cgttacggta tttctgatta cggcactttc atttacaagg
     5701 atgggcaagc acttcatgat cattcaaaaa ctgattgatc gtgtgaatag cattgcaaaa
     5761 gaaaacttac tagggattcg tgtagtgaaa tcttttgtac aagaagaaaa ccaattgaag
     5821 tcattcagca aagtaagtga ggacttaaca aaacacaaca tcatagtcgg tacattattt
     5881 tctgtgatga tcccgtcctt catgctggct gccaatttgg ctgtggttgg ggcgatcttt
     5941 tttgtgagcg atcttgttaa agatgatcct acgttaattg gtggaattgc ttcattcatg
     6001 aactacttga tgcagattat gatggcaatc attattggcg gaatgatgat gatgatgact
     6061 tcaagagcag ccgtatcgct gaagcggatt gcggaaattt tagatgctga accagatctt
     6121 acttatttag acgttccaga acaagagttg acgggttctg tcacttttga ccatgtttct
     6181 tttcgttatc ctggtgagga tacagatacg cttaaagata tcagcttttc tatcaaacct
     6241 ggagaaatgg ttggtatcgt cggggcgact ggtgccggga agtcaacact agcacaattg
     6301 atccctcgtc tatttgatcc gacagaaggc aaagttgaag taggtggtgt agatcttcgt
     6361 caagtaaatg aaaagagttt aagacaaacg gtttcctttg ttttgcaaaa agcaatttta
     6421 ttctcaggaa cgattgctca aaacttgcgc caagggaaaa agaatgcaac agaaaaagag
     6481 atggaacatg cttcttctat tgctcaagca aaagaattca tcgaaaagtt atctgatcgt
     6541 tacgaagcac caatagaaga acgtagcagt aacttttctg gtggtcaaaa gcaacgttta
     6601 tccattactc gtggcgttat cggtaatcca aagatcttga tcctagatga cagtacgagt
     6661 gcactggatg ctcgctctga aaagctggtc cgagaagcac tcgatcgaga tttgaaggga
     6721 acaacgacga ttgtcatcgc acaaaaaatt gcttctgttg tcaaagcaga ccgcatctta
     6781 gtattggatg aaggcagatt agtcggcgaa ggaacccatg aggaattggt agctggaaac
     6841 cgtgtctatc aggaaatctt tgaaactcaa aaaggaacgg aggagtaagt atgactgatc
     6901 taataaaagc aagtaaattt ttctatcact atttgaagcg gtacaaactc tcctttttct
     6961 ttatctttat cacagtgatc attgctacct atctacaagt aaaggcacct caatacatcg
     7021 gggaagcttt tcaagaatta gctaattacg taggaggctt gatgcaaggc gtagacgata
     7081 aaagcaaatt tgtttctgta atatggaaac tcttgctgtt ttatgtacta gctagtgcag
     7141 ccaactttat ctatagtatt ttgttcacac aagtagtagg gaagtcaacg aaccgaatgc
     7201 gtatcggtct attcaacaaa ttggaaaaat tgacgatccg tttctttgat tctcatcaag
     7261 acggtgaaat tttaagtcgt tttaccagtg acttagataa tattcaaaat agtttgaacc
     7321 aagctttgct tcaagtcatc acgaatgctg ttttacttgt cgggatcttg atcatgatgt
     7381 tccgccaaaa tgtagagtta gcttgggcta caattgcttc cactccagta gccgttttga
     7441 tagctgtctt gattatccgc aaagcacgga aatatgtgga tcttcagcaa gatgaagtgg
     7501 ggaaactaaa tggttatatg gatgaaaaaa tcagcggaca acgtgtcatc atcacaaatg
     7561 gcttacaaga agaaacgatt gaaggattct tagagcataa tcaaagtgtt cgcgaagcaa
     7621 ccttcaaagg acaagcttat tctggtctac tatttccgat gatgcaagga atgtcactgg
     7681 tcaatacagc gatagttatc tttttcggcg gctggttagc attaaatggt gatttggaac
     7741 gttcggtggc attaggctta gttgtgacat tcgtccagta ttcacaacaa tattaccaac
     7801 cactgatgca gatttcatct ggatacagca tgatccaatt agcgattaca ggggcaagac
     7861 gattgaatga aatatttgat gaaaaagatg aagtcaaacc agaaaacggt aaagcatttg
     7921 atggtgttga aaaaggtctt gcgttgcagc acgtagattt tggttattct ccagaaaaac
     7981 tagtcttgaa agatgtctcg atcaatgtgg acaaaggaga aatggttgct cttgttggtc
     8041 caacaggatc ggggaaaacg acgatcatga atctgctaaa ccgtttttac gatgtcaatg
     8101 atggttcagt agtcatcgat ggaatagata tccgagatta tgatcttgat gcattacgta
     8161 gacatgtagg aatcgttttg caagaatctg tattgttctc cggaacgatc aaagacaata
     8221 tcacattcgg caaaccggac gcttcagatg aagaagtcat caatgcagca aaacaagcga
     8281 atattcatga cttcatcatg acgttagaaa aaggttatga tacagagata tccgaagaaa
     8341 ataacttatt cagtacaggt caaaagcagt tgatcagtat tgctcgaacg atcatcacaa
     8401 atccatcttt attgattttg gatgaagcaa caagtaatgt agacacggta acagaagcaa
     8461 ggatccaaaa agcaatggat gaagcaatca aaggaagaac cagctttgtg attgctcacc
     8521 ggctgaaaac aatcttgaat gcagatagaa tcgttgtctt gcgtgatggg gaagtcattg
     8581 aagaaggcag tcatcatgag ttattagcag aaaatggatt ttatgcggaa ctttatcata
     8641 atcaatttgt atttgaaagt taaattaaaa gaacctcgtt ataacggggt tctttttata
     8701 tattttatgt tgtttgtttt tataaggggc agaaaagggg caatttaaat ttctattttt
     8761 cctagttttt ctactagttg attttgcata tttttagttg tatgagtata aatctttaat
     8821 gttgtttttg aatcttcatg tcctacacga tccataatgg cttttaatgg gattcctaat
     8881 tctgtaagta gagaaatgtg ggaatgcctt aacatgtgag aagaaacttt tttatctagg
     8941 tttaattctt tggatactgc t
//
LOCUS       DAYYGO010000109         8957 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000109 DAYYGO010000000
VERSION     DAYYGO010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.111"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            94..912
                     /gene="purR"
                     /locus_tag="ACHA5T_002309"
     CDS             94..912
                     /gene="purR"
                     /locus_tag="ACHA5T_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFM8887857.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            1415..2788
                     /gene="glmU"
                     /locus_tag="ACHA5T_002310"
     CDS             1415..2788
                     /gene="glmU"
                     /locus_tag="ACHA5T_002310"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFM8887858.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            2882..3853
                     /locus_tag="ACHA5T_002311"
     CDS             2882..3853
                     /locus_tag="ACHA5T_002311"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFM8887859.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      3859..3961
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4027..4596
                     /gene="thiT"
                     /locus_tag="ACHA5T_002312"
     CDS             4027..4596
                     /gene="thiT"
                     /locus_tag="ACHA5T_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFM8887860.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(4677..5450)
                     /locus_tag="ACHA5T_002313"
     CDS             complement(4677..5450)
                     /locus_tag="ACHA5T_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887861.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            5717..7397
                     /locus_tag="ACHA5T_002314"
                     /pseudo
     CDS             5717..7397
                     /locus_tag="ACHA5T_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
     gene            7367..7585
                     /locus_tag="ACHA5T_002315"
     CDS             7367..7585
                     /locus_tag="ACHA5T_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFM8887862.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            7605..8495
                     /locus_tag="ACHA5T_002316"
     CDS             7605..8495
                     /locus_tag="ACHA5T_002316"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFM8887863.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            8513..8797
                     /locus_tag="ACHA5T_002317"
     CDS             8513..8797
                     /locus_tag="ACHA5T_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887864.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
BASE COUNT         3019 a         1534 c         1882 g         2522 t
ORIGIN      
        1 ctattttctt attttttcat aaaatgctcg aatttttgaa gaaaaactta tataatgtaa
       61 aacgataaaa tttcttaaaa ggagttggaa taggtgaaag tacgtcgcag tgaacgtcta
      121 gttgacatga cacagtattt attgaatcac ccgcatgaat tgatttcctt aacaagcttt
      181 gccgaacgtt atgaatctgc taaatcttct atcagtgaag atttgggaat catcaagaaa
      241 acatttaaag aacgagggta tggaaccttg gaaacaatcc cgggtgctgc aggtggtgta
      301 ttgttcatcc ctgaaatttc atatgaagag gcgaaaaaat atattgaatc tctagcagaa
      361 cgtctatcag aacaagaccg tctgcttcca ggaggatatg tttatctctc tgatttgtta
      421 ggagaacctg aactgctgcg tcaagttgga agaatcattg cttctaaata ccttggtgaa
      481 aaaatcgatg ctgtaatgac tgtagccaca aaaggcgtac ctattgccca agctgtgtcc
      541 tattatctga atgcgccatt cgttattgta cgccgggact caaaaataac agaaggatca
      601 acagtaagtg tcaactatgt gtctggatct tctgaaagaa ttgaaaagat ggaactgtct
      661 aagagaagtt tgaaacgtgg ttcacgtgtc cttgtagtgg atgatttcat gaaaggcggc
      721 ggaacagtca acggaatgaa gagcttgatt gaagaattcg aatcagaact tgttggcatc
      781 actgtatttg ccgaatcaaa attcaacggt catcgcgcca tcgatgatta tacgtcactg
      841 ctttatgtca aagatgtaga tactcaaacg aaaaatatta cggttgtgcc aggaaattat
      901 ttttcagaat aagaggttgt gaaaaagctg cttagtttcg agtagtaaga aaaattttgt
      961 gaaaatggct ttccacattt tttcataaat ttggcttaat accgagaaac tggcttttga
     1021 acaccgttta tctagaggtt gtgaaatcag cgtcctgtcc tgagtattag ctcaataaac
     1081 ggaaaaattg ctcctcatat tttgccgttt attgagctaa taccgaaggt cagctgattg
     1141 aaaaacgttt aatgaagagg ttgtaagcct accgtttaac tctaagtcac aagaaacgat
     1201 ttcaaaaaac agcttttcat gttttatgag tagtgtgact tgtgaccgag gagttggcag
     1261 gagagcaccg gttaccaaat agataggccc gttatggtct atctattttt ttatccagaa
     1321 aacgctggga taaaagaaaa tcacttgttt tgttgaaaaa atgggtatac tgggataaac
     1381 tgaaatcagc aaactatatt taggagtgac gcagttggac gcacgttatg caattatttt
     1441 ggcggcaggg aaaggtaccc gcatgaagtc aaaattatat aaagttttac atcccgtttc
     1501 tggtaaacct atggtagaac atatcattaa tcgggtcagt gaaacgaaac cagatgaagt
     1561 gattacgatc gttggacacg gagcagaaca agttaaagca caactaggag aacgcagtaa
     1621 atatgcgtta caagcagaac aattgggaac aggacatgct gttttgcaag ctgcttcttt
     1681 cttggaagga aaaaaaggaa caacgttagt tatcagtggc gatacaccat tattgactac
     1741 tgaaacgctg aacaatcttt ttgaatatca ccaaggaaaa aatgcaagtg caacgatctt
     1801 gacggcacaa gcagaaaacc caacaggata tggacgaatc atccgtgacc atattgggat
     1861 cgtagaaaaa atcgtggaac aaaaagatgc aacaccagaa gaagctcttg tacaagaaat
     1921 caatacagga acttactgtt ttgacaacga agctttgttc gatgcgttaa gcaaagttgg
     1981 aacaaacaat gctcaaggtg aatactatct aacagatatc atcgaaatct taaaagaaga
     2041 aggccataca gttgctgctt atcaaacgga tgattttgaa gaatcaatgg gagtcaatga
     2101 tcggatcgcg ttagctaaag caaatgagat catgagaaag cgaattaacc agatgcatat
     2161 ggtcaatggc gtaagctttg tcgattcagc aacgacatac atcgatgcag gcgtagaaat
     2221 cggtccagac acattgatcg aagcaggtgt acaaatccaa ggaaatacag tcattggttc
     2281 tgattgtgtc atcgggtcac attcaaaaat cgtagacagt aggattgagg atcacgtcgt
     2341 cattgaaaac tcagtcattg aaagcagcca tgtcaagaaa catgcagatg tgggacctta
     2401 tgcacattta cgaccaaaag ctgaaattgg cgaaaacgtc catatcggaa actttgtgga
     2461 agtgaaaaat gctcaaatcg gcaaaggaac taaagtcggt cacttgactt atgttggtga
     2521 tgctacgctg ggagaagaaa tcaatgtcgg atgtggtgtt gtcttcgtca actatgatgg
     2581 aaagaacaaa catcatacga ctgtcggcga ccacagcttt attggttcta gcacgaatat
     2641 catcggacct gtcgaggtag ctaaaaacag ttcgattgct gccgggtcaa caattacaga
     2701 caatattcca gaatatgcgc tggcaattgc gagagcaaga caagtcaata aagaaggata
     2761 tgcaaaaaaa ctcccttatc taaactaaga cttttgcaaa atcacttgat ttatttaaaa
     2821 aatatgacta ctatttatta ggtagaattc tagagttgtg aaaaagaaag tggaggtccc
     2881 catgtcaaaa cattactttg acccaagatt aaaaattttt gcgttaaatt ccaatcgccc
     2941 attagcagag aaaattgcag cagcagtcgg cgtcgagtta ggaaaatcat ctgttactca
     3001 atttagtgat ggagaaattc aagtaaacat tgaagaaagt attcgtggat cgcatgtcta
     3061 tgttattcaa tcaacgagca gcccagtcaa tgataacttg atggagttgc tgatcatgat
     3121 tgacgcactg aaacgagcaa gtgcaaaaac aatcaacgta gtcatgcctt attatggcta
     3181 tgcgcgtcaa gaccgtaaag cacgtgcacg ggaaccaatc acagcgaaat tagtagcaaa
     3241 catgattcaa aaagctggtg caacaagaat gctgactttg gaccttcatg cagttcaaat
     3301 ccaaggtttc tttgatattc cagtcgatca tttgatgggt gcgccactta ttgccaatta
     3361 tttcttagaa aaaggaatca aaggcgatga tgttgtcgtt gtttcaccag accatggtgg
     3421 tgtcacacgg gcacgtaaat tagcggaatt tttgaaatct ccgattgcga tcatagacaa
     3481 acgccgtcca aaagcaaatg ttgcagaagt aatgaatatc attggacaag tcaaaggcaa
     3541 aacttgtgta ctgatcgatg atatgatcga tacagcaggt acgatcacat tggctgccaa
     3601 tgctttgaaa gaagctggtg caactagtgt gtatgcttca tgtacacatc cagtattatc
     3661 cggccctgct ttacaaagaa tagaagattc agctatcgag cgtctagttg ttacagactc
     3721 gatctatctt ccagaagatc gtaaaatcga caaaatcgat gaagtaagtg ttggcggact
     3781 aatgggagat gcaatcaaac gtatccatga aaacaaacca gttagtccat tgtttgaaac
     3841 aaaaaataaa taataaataa actcacaaag gggagtccaa ttgggctgag attgaatcta
     3901 tttctaaacc cttcgtacct gtatcggtta tgcgagcgta ggaattgtga ataaacagct
     3961 gtttgactat tttcgaaagc tgcttcctcc tttgtgactt ttttattcaa aggaggaagt
     4021 ttttttatgg gaaaaaatag agtttgggta gaaggaacga ttgttgcagc gttagcgatg
     4081 gtgttgtcgt tgattccgat ccagatcggc agcagttttt ctatttcatt agggcagatt
     4141 ccattgactc tttttgcgtt aagaagaggg acaaaggcag ggatgctggc aggtttcatt
     4201 tggggcatcc tacattttcc gttgggacaa gtttattatt taagtgtcgt tcaagtgttg
     4261 attgagtacc cgattgccta tacttttgca ggcgcagcag ggctcatggc ttcaaatgtc
     4321 caaaaaggct atgtagacga taatacgaaa caaattagaa aaagcatcgt gtttggtgca
     4381 ttgattggcg cactttccag atatttttgg cattttattg caggagtggt tttctgggga
     4441 gcgtatgcac tatgggggat gaatccttgg ctcttttctt tcgtaatgaa tggtgccagc
     4501 gggttgagta cagctattgt aacgatcatc gttttattag ggatccaaca aacgactcct
     4561 tcgatttttg tgccagataa acgacaggag ctgtaaataa aaaagaagca agaaacttga
     4621 gacagaatca tgtcctagtt tcttgtttcc tttttatttt ttgataagaa atagatctac
     4681 ttcaatggca gataagaaag atcggcatct tctaccaatt gccagtgtcg atcggtgaca
     4741 gagtagatta aagtagcgat gatttcctct tctcgatacc gggtgccttt taatttccaa
     4801 atataatctt caatcacata gattgggata cgatatttac catcgatcaa accaacttga
     4861 tcaaaagaaa aaatgatccc ttcctgcatc aaattacgca tttctttcaa taattccaca
     4921 ctgatttttt caacaggtgt ttcttttttg aaaaaagatt tttttacatt gatcttatct
     4981 gacaagatcg aacttaattt actagcaaaa atcctataaa attcatctaa atagcgataa
     5041 taaatccgat tcagctgatc attaagatcg aagtatacaa aattattctg tagtttataa
     5101 aaaaacggcg aatgaagatg tgttttcata tgaccaaaat ataataattc tgaaatttct
     5161 aaaggtgtca attctttcag catcgttaaa tcagtaaagt cgatccactt caattcatcg
     5221 aatccacgcc tgttacgaga agaggaaaag aaacgctgaa tattttctgt accacggatt
     5281 actttcagcc ctgtatggga ttcatattct ccagcagaag cagatggatc aagcaagagc
     5341 aagttttgag gaaaatgcac gatactgttt gcaaagtcag tagcagtcag cccttttgtt
     5401 aaaacagcat tacttgtggt atcaatatgt acataaactg actcagtcat catgttcctc
     5461 ctcttatttc ctcttaatct cttttatttt acaacatttg cattaaaaaa gcttgtttct
     5521 tttattttaa aattccatta caaaatcacg ttttcattga aaacactctc attttcaatt
     5581 aatttttgtg atttttgtat ttttttattt aaaggaggtt ttatgaattg ttttcttatg
     5641 gaatctacaa agtcgaatac gagactcatg acctttttat gacgtactgg tatacttgaa
     5701 gaaaaaggag agcgcgatgg agataaaaaa agtttacttt tcacagaaaa tgtcgctgta
     5761 ttcgcctatc cagcaagcaa aacaacccct tccatttttt caaagtcatc aagaaagtag
     5821 agacgctttt tggcgttctg caaaaaagca atgtgctttt aaagcagtat cgaaaaaata
     5881 taccgtctat tttactattg ctaaaagtag aggagatgtt atttttaata atctgttgat
     5941 tgaaggtgaa acttttgaat ggaaaaaatt ttttcggtat atggaagctt gcgctcgttt
     6001 ttttattaaa gaaaactgtt gttttttatt tcaatctcct ctatcagaag aatggttgcg
     6061 tatttttcat aaaaacggct atcaaggaac cacacaatta aataaaaagc tggtctatca
     6121 tacagcactt gttttaggtg gaggaggagc gcacggtgct tatcagattg gtgtgtggca
     6181 ggcgctgaag gaacatgata tcaactttga gattatcaca ggtacctcgg taggtgcgtt
     6241 gaacggcgca ttgattttac aaggagatat gaaaaaagct gtcgacaaga caagtgcttg
     6301 cgttaccaga aatggctgca gttgaagacc ttcgtgaacg cttctatcga gaaagacgcc
     6361 aaatgacgaa aacagcacta attgaaggcg gggtttcttc tgctccattg gaaaagatgg
     6421 tcaaagacaa tttggatttg tccttactca aacataatcc caaaatacgt ttatatacgg
     6481 tgtctactaa actgccagaa ctagcggaag tagtgactga tatccaacag acaaaaacag
     6541 aagaaatacc agattggata ctcgcctctg cttcatttta tcccgctatg gcttatcgaa
     6601 agatcggaaa aaataaatat gcagacggtg ggtatcgcaa caaaatacct attgatattg
     6661 cgattaacaa aggagcaaca gaggcatttg tcgtagatgt gcaagggcca ggcccagcga
     6721 aaaaaattcg tatgccagac atttttatcc attggaaatg tcaaacattg tggacactag
     6781 gcagctttct gctttttgat agccaaagga accaattaaa tttacaatta ggctatctgg
     6841 aaatgaaaaa acgattaggc tattactatg ggaactggta tacatttcat tctgttaaac
     6901 ctgcaagaac atactggcga ggatttttgt cttacttaac aaaagaaatt cagttagaat
     6961 tatctttttt caaaagcata aaattttggc aaaaactgcg taatttatat aagaacaggg
     7021 tcgtaccaga gacatgcgga ttggcgatgc tagaactttt agcaaaaaaa cattttttac
     7081 tgcccaatga aatctatcaa gtagacatga tgattcaaat gatctgccaa caagatataa
     7141 aaagtatgcc ggatgattta ttggcacaaa ttggtcaatt aagtactgaa gaatggcgaa
     7201 ggtatcgaaa ataccagcaa aaaatacaaa atgagcgcac gaaaactgtt tattttgatt
     7261 atctgctaca aggaaaacgc aaggatcgtt tgcagcatga attgcttaag caaccagttg
     7321 acacattact gatcctatat ttatattatc tcaaggagga acaaccatgg cacaagaatt
     7381 ttcatatgaa atcgtagaag aaatcgctat attgtcagaa aacaacaagg ggtggcgtaa
     7441 agaactgaac ctagtcagct ggaatggtcg cccgccaaaa ttcgatcttc gagattgggc
     7501 gccagaccat gaaaaaatgg ggaaaggcct gacattgacg aacgaagagt ttgaacaact
     7561 gcaaaaagca atagaaaata tgtaatgagg aaggaccctg tcgaatgaaa agtatgactg
     7621 gttttggcaa agccatccgc gaaacaaaag cgtatcagtt agatattgaa ataaaaagtg
     7681 tcaaccagcg ttttctagat gttcaaatac gaagtcctaa aatcctgaac tttgttgaaa
     7741 acgattttcg tcagcttatt aaacagcagt taagccgcgg tcgaatcgaa gtatttgtga
     7801 atctagctta tattggcaaa aacgaaaaac agatcgtcat caactgggaa ttgattgatt
     7861 cacttatcac tcaattgaca gacggaattc aacaacgata cggtagtcaa aatcaagtgt
     7921 caattagtcg gatggcggaa cgcttcgcgc tggacgaaaa cttcgtaacg gtagaagaaa
     7981 aaccggaaga gagcatggaa gaattggcac agttagctct agatacattg aaaagcgcac
     8041 ttgattctat tgaggcgagt cgagaaaaag aaggtcaagc tttagcttta gtgttgaaaa
     8101 agaatactga agaattcaaa gaagtactaa aaaaactcga tagttttgta gaaatctatg
     8161 aaaaggaatt tcaaataagg tatcaaaaaa agctggagga atttcttgga gcgaaagtcg
     8221 atgagcaacg gttgttgacg gagctagcca tcttgttgga acgcggagat attcacgagg
     8281 aactggatcg tctggcgatc catgtacaaa aactagatga acttatcgaa gcacaatgtc
     8341 ctgtaggtcg ggaattagat ttcctgatcc aagaaatgaa tcgggaaatc aacacgatcg
     8401 gttccaaatc aaatgcaatc gaaattaaaa atcaagtcgt ccagtctaag acgatcttag
     8461 aaaaaatcag agaacaagta caaaatattg aatgacgaaa gaggtagaga taatgaaata
     8521 ttatgacgta acgttccacg aattaagtgg aaaaactgtg gttaaacggg atatcccttc
     8581 tgaaaaaaac ggctttgacg tatggaaaga tgcctgtgca gactataatg agaacgaact
     8641 gtttattttg atcaacgacg gtgcttacgt gacgatgaat cggaaattta ttgttcgaat
     8701 tgacacagaa gaggtagaag atccgacaga aaaagcacgt tctcgaaaag atgagatcat
     8761 gggtgtagtg aatactttgt ccaatatggg cttttagcaa ggatgctgaa caaagaaata
     8821 caataaataa tcagaattgg atttcttcgc ttagcttctt tgctctagga atacatttaa
     8881 aggttgtgac aaaattcgtg tcacaatctt tttttatttt gaataagtga tgttcaaaaa
     8941 ccattttctc ggtatta
//
LOCUS       DAYYGO010000110         8777 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000110 DAYYGO010000000
VERSION     DAYYGO010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.112"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(375..2756)
                     /locus_tag="ACHA5T_002318"
     CDS             complement(375..2756)
                     /locus_tag="ACHA5T_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFM8887865.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2746..3393)
                     /gene="recU"
                     /locus_tag="ACHA5T_002319"
     CDS             complement(2746..3393)
                     /gene="recU"
                     /locus_tag="ACHA5T_002319"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFM8887866.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3457..3996
                     /locus_tag="ACHA5T_002320"
     CDS             3457..3996
                     /locus_tag="ACHA5T_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFM8887867.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            4086..4505
                     /gene="gpsB"
                     /locus_tag="ACHA5T_002321"
     CDS             4086..4505
                     /gene="gpsB"
                     /locus_tag="ACHA5T_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFM8887868.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4527..4894
                     /gene="rnpB"
                     /locus_tag="ACHA5T_002322"
     ncRNA           4527..4894
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHA5T_002322"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            5103..6269
                     /locus_tag="ACHA5T_002323"
     CDS             5103..6269
                     /locus_tag="ACHA5T_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFM8887869.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6315..7811
                     /locus_tag="ACHA5T_002324"
     CDS             6315..7811
                     /locus_tag="ACHA5T_002324"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFM8887870.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7892..8575
                     /locus_tag="ACHA5T_002325"
     CDS             7892..8575
                     /locus_tag="ACHA5T_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8887871.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            8584..>8777
                     /locus_tag="ACHA5T_002326"
     CDS             8584..>8777
                     /locus_tag="ACHA5T_002326"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887872.1"
                     /translation="MTEVVSSKIFRIIFRKSYFWNTVYQEVVSLPFSSESQGTISKNS
                     FSCLMNIVTCDSELAGEHRL"
BASE COUNT         2773 a         1586 c         1851 g         2567 t
ORIGIN      
        1 cagacaaatt atgataatta agcatactat cagataactc aaaatgaagc cttctttcaa
       61 aaaattattc aaaaccatat attgtatttt ttatttgata ataacaatgg gattattaag
      121 aagtggcact aaataaaaga ctagtgtgta tttagccgat ttaattataa acaagtaaat
      181 ggataaagta tatgtaatga taaaagaaaa atggattttc taatttttaa gctaaatgtt
      241 agatcacaca tgtatatgct ggatagtctt ataaaaaatc atgtaataca gcttggtaca
      301 tgatttttat ttgtagctgt tttttacata gacacaaaaa agaaaccacg cttgtagacg
      361 tggtttcttt tttactatct gtttccagaa gctgaggtgc tgctggatgg tgtatggtca
      421 tttgcagcat ccgctccgct agttgctggc tcactggaag aagcaggagg agcagatgaa
      481 gacggtgtcg agctttcttc ggatgaagaa gaaggaaccg ttgttgaaga cgctggctcg
      541 cttgtttctg ctgtcgtgct ttcttttgag gactcagcag ttgattcgct ttgactagta
      601 gagctagagc tttgagtcgt tgtctctgtt gttgagcttc caggagtctg gacatagctg
      661 gaagatggaa tagttgttga tggtgtaatt gcattcgatc tagctgtata ttgatctttg
      721 tagtacagtt caccgcccac acgaattaat ccgcttggca tctcccagtc tgtatttgtc
      781 acgttagctg aaacatactg cataagctcg cgataaacat cagaggctac gtgtgacgat
      841 tctgaagtga caggcgtcat ttttttattg taacctgtcc acacggaaat cgaataattt
      901 ggcgtatagc ccgcaaatag gatatctgga tacaccccag aagaaatacc aagttttgca
      961 tactcgtcat ctgtgtaatt ggatgttccg gtcttaccgg cttgatataa cccagcgatt
     1021 tgcgcattcg taccagttcc ttcagtgatc gtatctttta aaatgtctgt gatcatataa
     1081 gctgtttctg gagacatggc tgtttttcca tctggctcat aagtttcttc tgttccgtct
     1141 tggaagacga ttttattaac atattgaggt ttgtaataag tccctccatt ggcgaaagcc
     1201 gcataggctg ctgccatttt taaggaagaa gctccgtatt ttgtcccatc ttgttcttcc
     1261 gtattactag agatcgcatt cgattgatgg atcgtactgt attcgatccc aagatttttt
     1321 aagaaagaag ccactttatc tgaaccgact tcattgaata atttgactgc tggaacatta
     1381 cgggataagt aaagtgcttg gcgtaaagta atggtgccca tatattgatt atcccagttt
     1441 cctacaggag tcgaagtccc ttcatagtta tacggtgcgt cagtaatagt ttttccagta
     1501 gaatatttca aatattcaaa agctggtccg taatctgtga caggtttcat cgtagaacca
     1561 aaatcacggg aagtattcac tgctaagttg ttgcctaaag ttacgtcttc tgcgatgtga
     1621 cgtccgccaa tctgggcttt tactttcccg gtattggtgt cgataagggt ggaggcaact
     1681 tgcatctcgt cgtctgggta agagacatac tgatccgtgt tgacgatatc atatagtttc
     1741 ttttgggcgt ccagatccaa attcgtataa atctctaaac cgtctgtgta gacattctta
     1801 tctgtttttt cttgtacttc attgatgact tctttgacat aattatcgac gatcttagta
     1861 ttgtcatcgg attgagttag ttcttgcaga ccatccgtta ccggtacatt aacagcttga
     1921 tcatattctg tttgagagat tttttcgttt tgaagcatcg tatacaaaac agtatctcgt
     1981 cttttcttcg cctgatctgg ataaacataa ggatcatagg cggaaggggc ttgaggcatc
     2041 ccagcaagca atgctgtttg cggtaaagaa agttctgata atttcttacc aaagtacatc
     2101 tcagaagctg tctccatccc gtacaagccg tttgacatat acactttatt tacgtagtag
     2161 gtcaagatct cttgtttcga ttttttttgt tccaatctta cagccatcca cgcttcttgg
     2221 gccttgcgtt tcagagtctg atcctcggca ctggtggaga agaaggaaag tttgatcaat
     2281 tgctgtgtca aagtacttcc gccttgcagt ccgccagagg taaagttaga gagggcagaa
     2341 ccgatgatcc gaatagggtc aaccccgatg tgtttataaa aacggcgatc ttctaccgac
     2401 acgatcgcat cttctaatgt tttcggtaat tcattcgcag aaattttttc acgcttttct
     2461 gcgcctaaat cttcgaatag ttcgccatct tgtgtatata gtttggaaga tacggtagcg
     2521 tctaattttt tatccgtcaa ttcgggcgca tcttttgcgt aataccagaa aagtcctaca
     2581 cctgctaaaa agaggataca gaaaaatgag agtattccta aaaggatctt gataaataat
     2641 ccttttttat gtgttccaga ggactttcca gaacctttgc cagaattctt cttcacactc
     2701 tttttaggag taggctgttt ctgtcgtcta gagcttctag tttgttcatt tgccattctt
     2761 gctttctcct tcaatagata atgttgataa atagaagtca accgcgtcca aataaggaat
     2821 ccgcggagaa ataccagttt tgatctctat tcctagttct tcgatcaaag ataaaggtag
     2881 tgatttgcgt cctttttcct tttgggcgtc ccagtaagca atcaaatgaa cactattcaa
     2941 ataaaaacag cgcttcaaag acgaaaacca taacaagaca aagcagaccc cattttgttc
     3001 gaggcatgct ttcatatgtt cgatttgatg ggcatggaag tttttaaagg gaaaagatgt
     3061 tttgttcttg gtttcttttg cttcaaagtc caagtaaaag cctcgataga cgccattata
     3121 gtctgtagtt gaggcttggg taaagtaggc ttccttgatc acagcagcac ttctcttagg
     3181 atagtctact ttcacgattt ggataggggt aggcttttta tggataaccg ccaacccgcg
     3241 ggaaagataa tatttattac tttcattgat tgcttcttca aagcgcatcc cccgattgcc
     3301 gaattcagtg atctgatttc ttttttcttg aattttttcg acgggagagg cattcttata
     3361 ataaggttgg ccatttggat agcgcaatac catctttaat cacactccta ggccacatta
     3421 tagcaaaaaa gcggatagaa agaaaaggaa tctgagatgg ataagtcaaa agtaatctat
     3481 gtgtctgggt atcgaagttt cgaactcggt gtttttaaag aaaatgatcc taaaatccaa
     3541 gttgttaaaa atgttttaaa aaaagaactc catcagttag cagaagaagg gctggaatgg
     3601 gtacttgtca gtgggaattt gggcgtcgaa ctatgggctg tgcaaatagt agcggaattg
     3661 aaagaagaat atccggaact atctcttggt attttgtatc ctttttctga ttttggtaat
     3721 aactggaatg aagggaacca agaaaaaaaa caattagcag aacaattagc agattatgtc
     3781 gaagctgtta gccatcaacc ctaccaatca cctagtcagc taaagaatca cactcgtttt
     3841 atacttgagc atacagtggg ctgtctgctg atctatgatg aagaattccc tggaaaaacg
     3901 aagtattttt ttgaagatgc acagaattac caagcagatc atccctacga aattcggctg
     3961 atcagtatgg acgatcttca aaattttagt gaataatcga tattgtgttt gatttatcaa
     4021 cttttttcct atacaatgaa aaaggtagaa atgttattac gtaaatgaaa actagaggtg
     4081 taaatatggc gaatttggtt tatagtccta aagacatttt acaacaagaa ttcaaaacaa
     4141 aaatgcgcgg ctatgatccc gttgaagttg atgaattctt agacaatatc atcaaagatt
     4201 atgaaactta cagcaaagaa ctccttgctt tacaagaaga aaacgatcga ttgagtgcaa
     4261 aagtagctca attatctaaa actcaaggag cagctcaaac acgcgtgcaa caaacagagg
     4321 caccaaagag cgcagcagtg accaattttg atatcttaaa acgcttgtca aatcttgaac
     4381 gtgaagtatt tggtaaaaag cttgatcaac aagcaagtgc agtcaaacca gcacaaccta
     4441 atccaaataa ctacacaaat gcagacacaa gcttagatga taatgaaaaa acacgtcaat
     4501 tttaattttt gattaatgaa tttgcggttt tcgggtaatc gcggtctgac ttgatcaggc
     4561 tgaggaaagt ccatgctcgc acaggctgtg atgcctgtag tgttcgtgct tagcgaaacc
     4621 ataagctaag gtactctttg gagtaacggc aggaaaacaa gctaaggctt cggctatgct
     4681 taagtatcct tgaaagtgcc acagtgacga agcggctggg gaaacccatc cggtggaacg
     4741 cggtaaaccc ctcgagcgag caacccaaac aatggtaggg gcgctttttg tctggaagaa
     4801 gaacaggaca aaaaggcagt ataactgcag atagatggtt accgctttgt tttttctccc
     4861 tggaaaagca aagtacagaa catggcttat agaaaaccgc aaacatcagg gaattaggtc
     4921 gtgacaaaag taatgcgtta taagaaacaa gaaaaacatt tgaaagctat ttttcaatac
     4981 ttcagatgaa tcgttcttat tacaaaacaa acttttgtat caccggttat tcggaatttg
     5041 agtaaaagtg tggggtagga ctgatgtccc acctcctttt tttataaata aggagattgt
     5101 gtatggaaaa aaacaggaag ttcaacctcg tcgcaactgc ggcaagcggg ctagaagcac
     5161 tggttggtaa agaattaaga gatcttggaa tcgaatgcca agttgagaac ggacgtgcac
     5221 gttttgaagg aacaatggaa acgatcgcta cagctaattt atggttacgt acagcagatc
     5281 gagtaaaaat cgtggttggg gaattcgatg cgtatacgtt tgatgaattg tttgaaaacg
     5341 taaaagcatt gccttgggaa gattttcttc cactagatgc agcttttcct gtagctggaa
     5401 aatcgatcaa gtcgaaacta tatagcacac ctgattgcca agcgatcaca aaaaaagcaa
     5461 tcgttgaacg tctccgtacc tattatcacc gtccagcttc tgtaccactg acggaaactg
     5521 gcgcacattt tcagttggaa gtagctttgc tgaaagacca tgtgatggta actctggata
     5581 cgacaggacc aagtttattc aaacgtggtt atcgcttaga aaaaggtgga gcaccattga
     5641 aagaaaacat ggcagcagca cttattatgt tgacaaattg gcgaaaagac cgtccatttt
     5701 atgatcctgt atgtggttca ggtacgcttt gtattgaagc agccttaata ggccataata
     5761 tcgcaccagg attcaatcgt gagtttgctt gcgaatcttg ggattggttc agtcaagaaa
     5821 tcatggaaaa tgttcgatca gcagccgaag aaaaagcaga ttatgatatt gaattagata
     5881 ttactggttc agatatcaat ggacggatga ttgaaattgc caaagccaat gcagaagaaa
     5941 tcggattagg ctcttcgatt acctttaagc aacaagcagt caaagacttc aaaacggata
     6001 aagaatacgg ggtcatcgtg gctaatccgc cttacggaga acgtctagga gaagaagaaa
     6061 ctgttcggaa attatataaa gaaatgggcg atgtattccg tccattaaaa acatggagta
     6121 aatatatctt gactagcgac ttagcgtttg aggaatttta cggacaaaaa gcaaccaaaa
     6181 aaagaaaatt gtacaatggt gcattgcgta cggatctatt ccagtattgg ggaactcgtc
     6241 cgcctagaaa aaaagagaaa gacgaataat cttactaatt gattcattca attggatgtt
     6301 tggaggaata gggaatgaaa gaaaaagagt ttttacaaga agtaaaagaa atcaatcttc
     6361 tacaacaagc tttaggaatc ctcgattggg atacacaaac aggtatgcct gaaaaagcta
     6421 gtgcataccg aagtgaagta gacagctatc tttacagcat ttatttttca aaaaaaatcg
     6481 gtccaaaaat caaagaagcc attcgctatt tttctgaaca tccggaagaa ttgtcggaag
     6541 ttggaactgc cgtttttgcc aaagtaaaag aagagtatga tttggaacat cgcgtaccgg
     6601 aagatcttat gagaaaatat acagctgccg tttcttcagc acacggacaa tggcaaaaag
     6661 ctagagaaac acaacaattt tctgattttc aacacgcatt gaatcaaaat attgaattaa
     6721 caaaacagtt gataccttac tggagaaaag atgaaaagac gaattatgat gttttgctga
     6781 atcaatacga acccgggatg actgtcgaaa tccttgataa ggtatttgat cagcttaaag
     6841 aaggaattct ttcgatccgc cgtacgctcc aagaaaaagg gactgaacct gaaacagact
     6901 ttttaaatcg gagaatgacg aaaaaacaac agaagagatt tgtagtgaaa gtaatcgaac
     6961 aacttggtta tgattttagt cgtggtcgtt tagatgacac tgtccaccca tttatgactg
     7021 gaattaatcc gaatgatgca cgaatcacga cacgatggaa cgaaacagat ttcaaaatgg
     7081 ctgtttttgg agtgatccac gaggctggtc atggtatgta tgaacaagac atcgatgata
     7141 aatatgcgta tacaccggtt tatgaaggta catcaatggg tatccatgaa tcacaatcgt
     7201 tgtttaacga gatcattatt ggaagtagtc gcagcttctg gaaaaagcaa tatcctttct
     7261 ttcaagaatg tgcagaagga acattcgatg atattccttt tgacaaattt tatcgttcat
     7321 tgaaccatac aaaatcaagt ttggttcgta tcgaggcaga cagtttgact taccctctcc
     7381 atatcatcat tcgttatgaa atcgaaaaaa tgattttcaa tgaaggggta gatgttgctg
     7441 aactacctga aatatggaat caaaaatatg aagagtatct gggaatacga ccaacaaatg
     7501 atgtggaagg catcctacaa gacgttcatt ggtcaggtgc aagctttggt tattttccct
     7561 cttatgcatt agggtacatg tatgcggcac aattacttca ttctttgcag caagaaatag
     7621 atgtggaaaa tgttctagcg tcagacgatt attctccaat caaaaactgg atgtcagagc
     7681 atatccatca atatggtgca tctagaaaac caaatcaatt aatcatggat gcaacacatg
     7741 aaccgcttaa tccgcaatac ctgatcgact atatgaaaga cctgtatttt aatgtatatc
     7801 aggtaaaata aaatacaatt tacaaagata gctgaaattc atctaatcta tagatgtttt
     7861 cagctattta cttaagaaag aagtggaacg aatggataaa ttacaagtga tcgctgatta
     7921 tagtatggac caattagcgc atgatcaaac aggacatggg agcgatcata ccaaaagagt
     7981 tgtcaagtta gctgaaaaaa ttttggagac ggaaccgcag gcagaccgct ttgtgacgct
     8041 tgcagcagct tacctacatg atacgattga tgataaagtc gttaaaaatg agaacgaggc
     8101 aaagcaacag cttcgtgtat tccttcgctc tttgccaata gtagaggaac agatctcgat
     8161 gattttttcc attatcgaaa atatgtcctt ttctaaaaat ctctctgaag cggccgaact
     8221 atctctggaa ggtaagatcg ttcaagacgc tgatcgtatc gaagcattag gagcaatcgg
     8281 cattttgcgt actgcttatt tcggcggtgg acatgggcac ccgatttttg atcctgaact
     8341 gtatccgcaa acattcaagg acaaagaaaa ttaccgaaaa ggcacgacag tgatcaatca
     8401 tttttatgaa aaactgttct tgctgtctga taaaatgaat actgattacg gatatgaaga
     8461 agccaaacga agagaaacct tcatgcgtga attcttagaa gaattttttc tggaatggtc
     8521 tattgacgat gaaaatttta ctccagacaa ttggatcaaa aataacaaaa aatgatggag
     8581 gttgtgacag aagtggtcag ctccaagata tttcggatca ttttccgtaa aagttacttc
     8641 tggaacactg tttaccaaga ggtcgtgagc ctgccgttca gctccgagtc acaaggaaca
     8701 atttcaaaaa atagcttctc gtgtttgatg aatattgtaa cttgtgattc ggaattggca
     8761 ggagaacacc gtttatt
//
LOCUS       DAYYGO010000111         8771 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000111 DAYYGO010000000
VERSION     DAYYGO010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.113"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            110..1171
                     /locus_tag="ACHA5T_002327"
     CDS             110..1171
                     /locus_tag="ACHA5T_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFM8887873.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            1174..2070
                     /gene="murQ"
                     /locus_tag="ACHA5T_002328"
     CDS             1174..2070
                     /gene="murQ"
                     /locus_tag="ACHA5T_002328"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFM8887874.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            2084..4066
                     /locus_tag="ACHA5T_002329"
     CDS             2084..4066
                     /locus_tag="ACHA5T_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFM8887875.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(4131..4577)
                     /gene="nrdI"
                     /locus_tag="ACHA5T_002330"
     CDS             complement(4131..4577)
                     /gene="nrdI"
                     /locus_tag="ACHA5T_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFM8887876.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            4788..6212
                     /locus_tag="ACHA5T_002331"
     CDS             4788..6212
                     /locus_tag="ACHA5T_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFM8887877.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(6393..7166)
                     /locus_tag="ACHA5T_002332"
     CDS             complement(6393..7166)
                     /locus_tag="ACHA5T_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM8887878.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(7163..8068)
                     /locus_tag="ACHA5T_002333"
     CDS             complement(7163..8068)
                     /locus_tag="ACHA5T_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8887879.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(8222..8542)
                     /locus_tag="ACHA5T_002334"
     CDS             complement(8222..8542)
                     /locus_tag="ACHA5T_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFM8887880.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         2750 a         1576 c         1871 g         2574 t
ORIGIN      
        1 cctttagaaa tgaaatttaa ttattttaat aaaagtattg aaattttgtt tctttagata
       61 tatactctag tcatagaaat gaagacggat acagaaaaag agggataata tgtttggctt
      121 ttcaatattt atgaacgatt ctttgtcaga agagaagaaa cagtatatcc gccaaatggc
      181 atccaatggc tttgtcggta ttttcacttc catgcatata ccagaagatg atgtttcggc
      241 atatcaaaag cgattgatcg aactaggaag cgaagcgaaa aagaataaac tagaattgat
      301 ggtcgatatt tcaggagagg cattggaacg agcgggtttt tcattagaag atctaacgcc
      361 tttgaaagca attggtgtaa caggtttaag aatggattat catatttcca atcagcagat
      421 tgccgactgg tcacatcaat tgaaaatcag tttgaatgct agcacgatca cgcctaaaga
      481 tatcgatgaa ttgaaagaag cagaagctga tttctcacag attgaagctt ggcataatta
      541 ttatccacgt cccgaaacag ggctggacaa agaatggtat cagaaaaaaa atcaatggct
      601 caaaattcaa ggtctccttg tccaaggttt tgtaccagga gatacagagt tgagaggccc
      661 actatatcaa ggattgccaa ctttggaaga acatcgaggg atccatcctc ttgcaggcgc
      721 tctagatcta ttagcaagta acacggatat tgtttacatc ggagatgcag gtctgtcaga
      781 aaacgttcag gagcaatttg cttctttcca aaaagaacag acagtcttgc tccatacgga
      841 acctgtagat gaagaatttt acgaatatat attagggaag catacgaatc ggcaagacga
      901 tgcaagagat gtcatacgaa gcgtagatgc acgttttcga gagattccgc ctatcccagc
      961 aagaaataca gctacgcgta tgaaagggtc tattacttta gataacgaaa agtatttgcg
     1021 ctatatgggt gagatccaat taaccaaata tgatttgcca gcggatgaaa aagtgaatgt
     1081 agtagctaaa gttatcaaag aagagctacc attgatcaac caaatcaaag ctggtatgaa
     1141 ctatcaattt ataagaaaag aaggaagata aaaatgagca aaatcaattt agagaaccta
     1201 acaacggaac gcagaaacca agaaactttt ggattagatg agatgagtat ttctcaagcc
     1261 gttcaactaa tgaacaaaga agatcagaaa gtggccgctg cggttgaaaa agaactgaca
     1321 agtatcgaac cagtgattgc taacacgatt gagtccttta aaaaaggtgg acgtctgatt
     1381 tatatgggag ccggaacaag tggaagacta ggtgtgttag atgctgctga atgcgtaccg
     1441 acattcggtg tagaaccgga aatggtcata ggtctaattg ctggtggaga agaagcaatg
     1501 actgtagctg tcgaaggggc cgaagattct tatgagctag ggcaagaaga tctaatcaag
     1561 ctgaatttga caaaaaatga tatagttatt ggtatcgcag ctagcggtcg tacaccatat
     1621 gtgatcggtg gattagatta cgcaaaatcg atcggtgctg tcacaggaac gatttcttgc
     1681 aataaacaag ccgaaatcag taaacacgcg gatttcccaa tcgaagtcga tgcaggtcca
     1741 gaatttttga caggctctac tcgattgaag tcaggaacag cacaaaaact tatcttgaat
     1801 atgatttcta ctatcagtat gattggtata ggtaaagtat tcaataactt gatggtcgat
     1861 gtcaaaccaa caaatgaaaa actaatcgaa cgctcaaaac gaatcatcat gcaagcgaca
     1921 gaagctgatt acgaaacagc tgaaaagtac tttgaagaag cagatcaaaa tgttaagctg
     1981 gctatcgtca tgattttgac aaatagtgat aaagaggaag ctcaagcaaa attagctgaa
     2041 gcaaacggat ttgtaaaagg gaccttatga gggggaaaag accatggcaa aaaacgaact
     2101 gactttggaa gaactggcaa aacaaattta taaaggggca ggcggcatgg gcaatgtaga
     2161 aagcattgtc cactgcatga ctcgtgtgcg tatgaagatc atcaatgatg atcttgtaaa
     2221 tgtctctgaa ttaaaagcga ttccaggagt gcttggtgta gtagaagatg aacagcttca
     2281 agtcatcatc ggacctggga aagtgaacaa agtagcacag caaatggttg atttggcagg
     2341 cgtgaaatta ggagaaaaac tttcttctgt aagtacagct acagcaaatt catcagcatc
     2401 tggaaaagac cgtgtaaacg aacgggcaca agaaatgaaa gcggcacaaa aatcgaaaca
     2461 aaaaccatct aaaatcaaat cggtattaaa agacatttct aatatctttg tgccaatgat
     2521 tcctgcattt gtcggtactg ggatcgtggc agggattgcc gcagttttag caaacttagt
     2581 cacagccgga acacttgatg cagcaacatg gcaacaatat atcgatgtga tgaacatctt
     2641 gaaaaacggg atgtttagct acctagttat ctatacaggt atcaatgctg cacaagtttt
     2701 tggtgcaaca ccaactcttg gtggagttat cggggccgtt gtgatgctga cagggatgaa
     2761 tccggaagca ccactgaaaa acttatttac tggtgaagct cttgcagctg gacaaggtgg
     2821 gatcctaggt gtcatcttcg ctgtttggct attgtcaatc gttgagaaaa aactgcataa
     2881 agtagtacct gatgcggtag atatcatcgt tacaccaact ctttcattga ttgccatcgg
     2941 tctgattgaa attttcttca tcatgccttt agcaggtttc atttctgatg gcatggtcgg
     3001 tgggatcaac tgggtacttg gcgttggtgg agcatttgcc ggcttcgttc taggcacatt
     3061 attcttgccg atggttatgt tcggactgca tcaaattttg actccaatcc atgtgcaaat
     3121 gattgatgaa actggtcgta cgctattgct tccaattctt gcgatggctg gtgcaggaca
     3181 agtgggtgca gcattggcat tatggattcg ctgcaaaaaa gataaagaat taacagaaat
     3241 gatcaaagga gctttaccag tcgggatcct aggaatcggc gaaccattga tttacggagt
     3301 aacattacct ctaggacgcc catttattac tgcttgtatc ggtggaggta tcggcggggc
     3361 agtgatcgga atgatcggaa atgtcggggc tatcgctatt ggaccttcag gagccgcttt
     3421 gatcccattg atctctgacg gtaaatggtt aggctacatc cttggattat tggcagctta
     3481 tgctggtggc ttcgttgcta cattcttctt cggtattcct aaagaccgtt tagaaaaaga
     3541 agaattagcg actgaaacag ccacagtaaa tgaagtgcct gaaaaagtag tacctcaaac
     3601 ttcagctgaa agtgtgacta tcctctcatc agttgctgac ggaacagttg tttctttaga
     3661 taaagcaagt gatccagttt ttgcacaaaa gatgatgggt gaaggctact ttgtagaacc
     3721 agaaaacgga catatctatt ctccagttac tggggtagtc agctctgtct tcccaacgaa
     3781 acacgctatt gggatcacaa cgccaaatgg tttagaaatc ttgcttcata tgggaatcaa
     3841 cacagttgac ttaggcggta cgccatttga tgtgaaagta acagaagggc agcaagtgac
     3901 acctgatacg cttgtagcag aagctgactt agaagcgatc aaagcagcag gcaaagaaac
     3961 atccatgatc gtcttggtaa ccaatatgga tcgtgtaaaa aactttgtct tggaaaaaac
     4021 aggtaaagca aaagcaaaag cacctgtgat ggatgtcaaa gcttaatatg atagaaacaa
     4081 tagaaacgaa agaagaaact gaaatctatt tttcagcttc ttctttttta ttatttattg
     4141 ttttctgcta aaatctgata aattcgtgtc acatcagatg gtgttccccg taattcataa
     4201 tctgccaaga aaggaaagcc aaatgtttgt gcatattgtt tggctgttag acaatactga
     4261 ttattgaagt ttttatttcc gctaccgact acaccaagac aaagtttatg attgtcttca
     4321 tgttccagat attcacgcat cgtttcggtc aagatttctg tatcaccgtt atctaaacca
     4381 tttccgccgt ctaaataagt aggaacgaaa gtaaaaaatg gttctgtttc tttcgtaaaa
     4441 tcactatttt catggatttc ttttaaagta acttcaggat tgacaggatc ttctgcatgc
     4501 attttttcag catattctgc tagatgtttg gcaaaggcac gcgtatttcc tgaaatcgat
     4561 atatataaaa tgttcatgga tcatcctcca attctttcct tttagtatag catctttttt
     4621 aagttttttg gtggtgtaat ttggaaaggg actaggcgct ttgcttgtat aatgagaaaa
     4681 aagccggtag aattatattt ctattaaagt ttttattaat taagtaaaaa taaaaaaggt
     4741 ttgttatact ttttgataaa agcggtttta ttttggagga tgcttacatg gatacagagc
     4801 aacagaaaaa aggaattggt ttaattccac ttgctgcctt agtaattggt tcagcgattg
     4861 gaggtggcgt atttggtatt atgacggaca tctccggatc ggctggcggc ccagctattt
     4921 tcagttgggt actcgtagga acagcaatgt tgatgctctc cttgtcgatc aataatatca
     4981 ataaaaaacg tccagattta aaaggcggga tctttagtta cgcagaagaa ggttttggcc
     5041 gtttcgctgg ttttatcagc ggttggggtt actggctcac atgctggtta ggcaacgtag
     5101 cttttgcaac attgttgatg agcgcattag gatatttctt cccagtattt gaaggtgggc
     5161 aaaatctggc atcgatcgtg atgagtacag tctttttatg gggatatgca tggctagtga
     5221 atcggggagt cgaaaatgct agtttcgtca atgctatcgt cacattctgt aaattagtgc
     5281 cattattttt atttattgta gtagctatca gcacgttttc gatccaacaa ttcattgata
     5341 acttttctag cgacattata ttgaatgcga atggacagcc gatcccgttt tccagtcaag
     5401 tattgagttg catcatggtg atgctttggg tatttgttgg tattgaagga gcctctgtcg
     5461 taggttcacg agcaaaaaaa caatcagatg ttgggaaagc aacgattcta gggatattcg
     5521 gcttgattat tatttacgtc ttggtttcca tgcttccata tgggacaatg acgatgaatc
     5581 agctgcaaac gatcaaggcg cagcctgcta tgggctatgt gtttgaaatg atggttggta
     5641 aatggggagc agtcgtgatc aacggcggtt tgatcatttc tttagttggt gtttggctct
     5701 catggacgat cttaccgatt gaaacaatgg gaaacatggc tgaagacgga cttttgccta
     5761 aagcatgggg aaaactcaac aaaaatgggg caccaactta tgctatcgta ttgacgacgt
     5821 tatgtacgaa tgtcttttta ctttcattat tattcactga cagtgcttat aactttgctt
     5881 acacacttgg tacagcagct attttcttca cgtggctttt cttaggattg taccaaatga
     5941 aactttctta tcagcggaaa gaatgggtcc aatttttagt agggctttta gcaagtgcct
     6001 ttcaagtatg ggctatgcta gtcgtagcct ttaatgaagt aatgcttgtt cttgttcttt
     6061 tcttgccagg catttatttt tacctcaaag cacgtaagga gcaaaacgag cctgtacaac
     6121 atatccactt agataagatt ttacttatag gtgtgacgat ggtcggagtg attgcaatgg
     6181 ttttatttgc cactgggatg cttcacgtat agaagtataa aagaagtata aagagaggtt
     6241 gtgagcctgt cgtttattca aaaacatact aggaaaggag ctgtgacaca aatgttgtca
     6301 cagctccttt ttcagaataa acggtgttta agagacagcc aatcggcaat aaggctgaac
     6361 gtctctttca cagcctcttt tttgatgagt catcatacat tttttcgctt aaatacgaga
     6421 tatcctaatg ctaagaacaa aacgatgtaa gctaaattac cgaatagcat ttgattagta
     6481 gaaagatgag tcaattgctt catgatctct tcgtttccaa tttggttttg cagattcaac
     6541 atactgatcg gattccattt gatccactcc catttttgga tgagtgcaat aaggatacca
     6601 gatatgattg acgaagcaaa ataaaagacg attcctgctg agatggatgc gccggaacta
     6661 ttgataaagc aagcaatcat caacactaaa cttaaaatca gccataaacc gatataacta
     6721 cccaaagtat aagtgaacaa gctttggatc agtgtttgac cagtagaaac cttctccgtg
     6781 aaggaaatcg aagggaaaag aatcagtttc atcaacacag tgacaataat agctaaaagg
     6841 tataaataaa cagaataaat aactaatgtg atccatttgc ttactaatat ttctcctcga
     6901 gaatactttc gataaaggag atttttcaat gtaccatatt gtgcttccat cgaaatgatc
     6961 gtgcttgcag ctgcaatcat gataaagaca atccaagatg tggcactaaa aagctgagca
     7021 gtcatttcaa ctggatcaat gatcgtcgtg tattttttgg ctagaagtgc tgttcctatg
     7081 agcaaaacaa ctaatagaac agataaaaca gcagttgact ttttcttgat caatttaaat
     7141 aattcttgtt ttacaagcaa tgtcattttt cttcttcccc ctttactgaa tccagaattt
     7201 ccaatagaga agattccaaa tcattcgtca gatgctggac gtctgtcaca tggatatttt
     7261 gtgcagacaa tagctggatc acggttccta ataattcttc gttttctttt aaaagcttga
     7321 ttttatcagg atcttccaaa gtatatccag cttctgtcaa gatcgttcga gcagctagat
     7381 catttgttgt agagataacg atgaaatctt gcgctgtttg gttcaattca gccattgtac
     7441 attgtttgat gatctttcct tgattgatca ggatcagctc atcgaccatt ttttcaagct
     7501 cgctgagtaa gtgactggag atcaagaaag aggtgccctg agtagccaag tcggtaatga
     7561 tttttcgcac atctcttgtt gcttgtggat ccaaaccgtt catcggctca tctaaaataa
     7621 cgatagacgg atggttcagt aaggcaagag caattcccaa tttttgtttc attcccaacg
     7681 agtaggattt cgcttttttg tgaatgtact tttccatctt gagctgattc acgatagtgt
     7741 cgatagcaga tacatcgttg gtttcagaga ataacttcag atgatcatag cctgttaaga
     7801 atggatagat acccgggtat tcgatcaatg ctcctacttg ttccaaccct ttatgactag
     7861 agggagaaat ctcattttca ccaatacaga ttgttccatc tgagtaatgg atcagtccta
     7921 aaatagcttt catgatcgtt gtttttccag cgccattagg acctactaat ccaacgattt
     7981 tgccagggga taagctaaaa ctgacatcgc tgagggcttg tttgtgacca aagttcttgg
     8041 ataattcttg gaccgaaaga cttttcaaaa atattcactc cttttctata gatagtacaa
     8101 gcatacacct gatgagtgga gaagtcgcta ggtcattgga cttatttctt attttttcat
     8161 aaaaaaattg aatattaaat aaaaaggctt attttttctg atcagtactt attccaagtg
     8221 tctagtggcc gctagtaatc ttgatgccaa tgatactgat gagtagcatc cccacaaaga
     8281 accaagtgga aagattcagc ttatcatgaa acaagaccac accaattatg accgaaccaa
     8341 ctgcgccaat ccctgtccag ataggataag agagactcat cggcaaactt ttgattgcct
     8401 ttgacaaaaa gtaaaaacta gcaatcatgc caatcactgt gtaaaaagaa taattgattt
     8461 tagaaaaacc atcactccat ttcatcatcg ttgaccagaa tacttccaaa attcctgcta
     8521 tgattaattc catccatgcc atacttacat tcctccttaa ctataaaaaa atacacaaaa
     8581 agaggaaaat catcccttca aagacctaat ggaatgatca tcctctttat cctacccggt
     8641 ggcagattgt atatcttttt ctgtagtata gcacacctat ccagatatcg gcaatctcat
     8701 actttttagt aaagtgtacc aagcactgac ggatatatcg ttagcggaaa agaagatgaa
     8761 tgctttgcaa t
//
LOCUS       DAYYGO010000112         8493 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000112 DAYYGO010000000
VERSION     DAYYGO010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.114"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..149)
                     /locus_tag="ACHA5T_002335"
     CDS             complement(<1..149)
                     /locus_tag="ACHA5T_002335"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8887881.1"
                     /translation="MENHVLETGMQKITTHASITAKPFFEKRGYKVINEQTVELRGQL
                     FTNFL"
     gene            complement(593..1714)
                     /locus_tag="ACHA5T_002336"
     CDS             complement(593..1714)
                     /locus_tag="ACHA5T_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFM8887882.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="ACHA5T_002337"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="ACHA5T_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFM8887883.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="ACHA5T_002338"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="ACHA5T_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HFM8887884.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="ACHA5T_002339"
     CDS             complement(4304..5215)
                     /locus_tag="ACHA5T_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8887885.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="ACHA5T_002340"
     CDS             complement(5232..6239)
                     /locus_tag="ACHA5T_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HFM8887886.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSIPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="ACHA5T_002341"
     CDS             complement(6236..8365)
                     /locus_tag="ACHA5T_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM8887887.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8480)
                     /locus_tag="ACHA5T_002342"
     CDS             complement(8370..8480)
                     /locus_tag="ACHA5T_002342"
                     /inference="COORDINATES: protein motif:HMM:NF024254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887888.1"
                     /translation="MMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2600 a         1727 c         1486 g         2680 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggaattga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg ttt
//
LOCUS       DAYYGO010000113         8312 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000113 DAYYGO010000000
VERSION     DAYYGO010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.115"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(105..629)
                     /locus_tag="ACHA5T_002343"
     CDS             complement(105..629)
                     /locus_tag="ACHA5T_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFM8887889.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="ACHA5T_002344"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="ACHA5T_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFM8887890.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="ACHA5T_002345"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="ACHA5T_002345"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFM8887891.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="ACHA5T_002346"
     CDS             complement(2351..3031)
                     /locus_tag="ACHA5T_002346"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFM8887892.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="ACHA5T_002347"
     CDS             3157..4026
                     /locus_tag="ACHA5T_002347"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8887893.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="ACHA5T_002348"
     CDS             4052..4567
                     /locus_tag="ACHA5T_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFM8887894.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5177)
                     /locus_tag="ACHA5T_002349"
     CDS             complement(4614..5177)
                     /locus_tag="ACHA5T_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8887895.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="ACHA5T_002350"
     CDS             complement(5232..5411)
                     /locus_tag="ACHA5T_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887896.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="ACHA5T_002351"
     CDS             complement(5488..5997)
                     /locus_tag="ACHA5T_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887897.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="ACHA5T_002352"
     CDS             complement(6093..6473)
                     /locus_tag="ACHA5T_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8887898.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /gene="pnuC"
                     /locus_tag="ACHA5T_002353"
     CDS             6803..7552
                     /gene="pnuC"
                     /locus_tag="ACHA5T_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFM8887899.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7873..>8312)
                     /locus_tag="ACHA5T_002354"
     CDS             complement(7873..>8312)
                     /locus_tag="ACHA5T_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8887900.1"
                     /translation="KKAEGSPCFLLYQTFPGIGALTAALLLGELGDITRFKTHKQLNA
                     FVGIDIRRYHSGKYTGQDRINKRGNPKARKIIFFTIRNMIRQQRAAPNHIVDYYYKLK
                     KQPIPKKDKVATVACMNKLLKCMHAMVRAHTEYDYAYAVSVDH"
BASE COUNT         2374 a         1714 c         1500 g         2724 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aatcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     7681 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct atttcctacg
     7741 attagtcaag taaaaggtgc aagaaaaata gctcgtaaac gaaagcgaca aaatcaaagc
     7801 acaccaaata aagccccgtt cgtaaaaaat tttcgatctg ggcaaagaag tggtatactg
     7861 taagtggttt agttaatggt ccactgagac cgcgtacgca tagtcgtact ctgtatgtgc
     7921 cctaaccata gcgtgcatac atttcagaag cttgttcata caagcaactg tggcaacctt
     7981 atctttctta gggataggtt gcttttttaa tttgtagtaa taatcgacaa tatgattagg
     8041 agctgcgcgt tgctggcgaa tcatatttcg aatggtgaag aaaataattt tccgtgcttt
     8101 gggattacca cgcttattga tgcggtcttg gccagtatac ttacctgaat gataccgtcg
     8161 gatgtcgatt ccgacaaagg cattcaattg tttatgggtt ttaaatcgtg tgatatctcc
     8221 taattcaccg agcaataaag cggctgtgag tgcgccaatc ccagggaacg tttggtacaa
     8281 caaaaagcaa ggagagcctt cggctttttt ga
//
LOCUS       DAYYGO010000114         8298 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000114 DAYYGO010000000
VERSION     DAYYGO010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.116"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            476..1339
                     /locus_tag="ACHA5T_002355"
     CDS             476..1339
                     /locus_tag="ACHA5T_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8887901.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="ACHA5T_002356"
     CDS             complement(1468..3090)
                     /locus_tag="ACHA5T_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8887902.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="ACHA5T_002357"
     CDS             3339..3860
                     /locus_tag="ACHA5T_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFM8887903.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACHA5T_002358"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACHA5T_002358"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFM8887904.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="ACHA5T_002359"
     CDS             5941..6759
                     /locus_tag="ACHA5T_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFM8887905.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKHHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="ACHA5T_002360"
     CDS             complement(6862..7059)
                     /locus_tag="ACHA5T_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887906.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="ACHA5T_002361"
     CDS             7228..7806
                     /locus_tag="ACHA5T_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFM8887907.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="ACHA5T_002362"
     CDS             7901..8038
                     /locus_tag="ACHA5T_002362"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887908.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2500 a         1488 c         1634 g         2676 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacat cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaataa aaaattagtc
     8221 aaagaagggc gtatagattt cttttgatag gagatataca acagaaatga ataaatatta
     8281 acgtgttttc aaacagta
//
LOCUS       DAYYGO010000115         8249 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000115 DAYYGO010000000
VERSION     DAYYGO010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.117"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(204..1652)
                     /gene="cls"
                     /locus_tag="ACHA5T_002363"
     CDS             complement(204..1652)
                     /gene="cls"
                     /locus_tag="ACHA5T_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFM8887909.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGEIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            complement(1804..2688)
                     /gene="ftsX"
                     /locus_tag="ACHA5T_002364"
     CDS             complement(1804..2688)
                     /gene="ftsX"
                     /locus_tag="ACHA5T_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFM8887910.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            complement(2681..3367)
                     /gene="ftsE"
                     /locus_tag="ACHA5T_002365"
     CDS             complement(2681..3367)
                     /gene="ftsE"
                     /locus_tag="ACHA5T_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFM8887911.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(3472..4573)
                     /gene="prfB"
                     /locus_tag="ACHA5T_002366"
     CDS             complement(join(3472..4500,4502..4573))
                     /gene="prfB"
                     /locus_tag="ACHA5T_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFM8887912.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(4699..7233)
                     /gene="secA"
                     /locus_tag="ACHA5T_002367"
     CDS             complement(4699..7233)
                     /gene="secA"
                     /locus_tag="ACHA5T_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFM8887913.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            complement(7455..8006)
                     /gene="raiA"
                     /locus_tag="ACHA5T_002368"
     CDS             complement(7455..8006)
                     /gene="raiA"
                     /locus_tag="ACHA5T_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFM8887914.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            complement(8134..>8249)
                     /locus_tag="ACHA5T_002369"
     CDS             complement(8134..>8249)
                     /locus_tag="ACHA5T_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HFM8887915.1"
                     /translation="KILIIDDVYTTGQTLFHAADCLQNAQPKIIRTISLAR"
BASE COUNT         2200 a         1816 c         1440 g         2793 t
ORIGIN      
        1 gctatactgg ctttgaaatg tgtctctttt aatagcatat attagcttat ttcttgcttt
       61 ccccaaaaaa caagagatac cccaaagtta atttcgctca tgcgagacgc tttctccatt
      121 tctgacgtac tctcagaaat ggccccgtta aaaaaggaca cgagatctcc atctgttgat
      181 ggttctcatg tcctttttat gttttaaagg agcggcgaaa tcaatcttgc taatccttct
      241 ttcatcttga tgattattcc tcggttttca tacatttctt ttgtgagatg cgtagaaatt
      301 ttcgagtctt caaaaaaagc ttttcttaca cgtgaagcca ttcgttcatc ataaaccagt
      361 gtattgacct caaagtccaa gcgaaatgaa cgaacatcga tatttgctga gccaactgaa
      421 acaatgccac catcgataat catcgtcttt gcatgaataa atccattctc atatgtttcg
      481 atccttgctc catactcgat cagctctgca gcgaaagaat acgttgccca gtaaacgaga
      541 agatgatctg gtttgttagg tatttggata tgaactttta cgcctgaaag aagagccaac
      601 ttcagtgctt catgaatcga tccatccggt atgtaataag gcgtttggat caatatctcc
      661 cgtttggcta acgaaatcat tttcaaataa gtcattttga tttgttcatg ttcggtatct
      721 ggtccgcttg tcacgatttg aacagctata tcaccagtag tgtgaatgga cggaaaaaat
      781 ttgggagaat agcctacttc ttttgtatgc tgagaattcc agtccatcaa aaaacggttt
      841 tgcaaggtat acaccgcttc tccgtaaata cgcaaatgat tgtctcgcca ataaccaaat
      901 ttctttacca atcctagata ttcatttcca acattgaacc cacctgtata tccgatttcc
      961 ccatcaataa ctacgatttt acgatgattg cgataattga tccgaggatt aatatacgga
     1021 acaaatagag gaaagaaaaa tgcgacttct ccgcctgctt ttttcaaatt ctgaaaaaag
     1081 ctggaagaaa cttgtgtgga accccatgca tcaagaagga ctctgacttt tacccctcgt
     1141 cttgccgcct ctgtcaatgc atctcgaact tcttcaccta atgcatcact gcgataaata
     1201 taatactgga agtggatatg gtcttctgcc tgcttgatat cttcaatcag cgcatcgaat
     1261 ttttcacgac catctgtaaa taaagtgatt tcatttttag tcgtatagag cgtactttca
     1321 aataccgtga gcatatagat caattgtttc acatctactt gtccagtagg cggatgagga
     1381 taaagccctc gcatcaatgc ctgtttgtct tcttcaattt ccaggttctt accgattttc
     1441 ccttggatct tcaaatcaaa gattttctct ttggaaatcc ctcttcctaa aaaaatatat
     1501 aaaataaatc ctacaactgg aatgaacatc aaaacgagca gccatgccca tgtttgagca
     1561 gtttcctttc tttctcgaaa aacgatgata aaggaaagca aaatattcat aatcaaaagg
     1621 atcgtaaaaa aattgtcaac gataattttc acggcttcac ctcttcttct accaacttaa
     1681 gaatacccaa gttgtttttt aaagtaaagg gaaaaaagcc gcttccttct attctcctag
     1741 ccaataaaaa aagaaagctg gaacgaaact gtccaacttt ctcattttat ttatattgtg
     1801 gtattagaat ttcaagaaac gtctcataga aatgatcgag ccaagtgaac caatgatcac
     1861 accaatgccg accattaaaa gattgatttt ccaaacgata ttttgtggat gaatcaatga
     1921 ataatgggaa cgtaataatg atggattgat caattggaaa aaccatgcat aaccaaatgt
     1981 gatgatcaaa attggaagaa ttgcaccgat cagaccgatc cagccacctt caaggaaaaa
     2041 cggccagcga atatatccat tttttgcacc gactaaacgc atgatttgaa tttcacgctg
     2101 tctagacaaa atcgtgatcc gaatcgtgtt cgaaatgaga aacatcgcta cgaacagcag
     2161 caatgccgct gcaactaatc cccatgtccg aactgcacca gcgatttgga aaatccgatc
     2221 tgaggaagct ccaccgtaat cagaacgaga aacattcttc aaatctgctg cttcttctgc
     2281 tacttgtttt gtataagaag gatcagtagc acttactaca tacacatcat ataacgggtt
     2341 attgtcacct tcaaacaatt tccaggaatc acccatttcg ttttggattt ttttcaattc
     2401 ctgttctttg cttgaaaatt caactttttt aacatgatct aagtcattca attctttttc
     2461 tagctttttc atctcttcgt cttttgtacc gatatctacg aatacagata catcaacgtt
     2521 tccttctata tcctgagcta atttagttgc attcatgata actgccatga atacaccgac
     2581 taaagtcaac gtgatcgtta ccgcactgac tgaagcaact gtcatccagc cgttacgttt
     2641 caaactcttg atactttcta ataaatggcg gaaaaaggtt ctaatcatcg tatccatatt
     2701 ccccttccgc ttgatctcga ataatgcggc cgttttcgat agcaattact cggtgccgaa
     2761 tcgtatttac gatcgtactg ttatgtgtag ccatcacgac agtagttcct tgggcattga
     2821 tccgatctaa taatttcatg atttcccatg agttttctgg atccagattt cctgtcggtt
     2881 catccgcgat aagtacttta ggcgtattca cgattgcccg agcaatagag acacgttgct
     2941 gttccccacc ggataattcg ctaggaaaga ctcgaacttt gtgtttcaaa ccaacaaggt
     3001 ccagcacttc cattactcgt tttttgatat ctcgaggttt tctgccgata acttgcatag
     3061 cgtacgcaac attttcatat actgtttttc ttggtaataa tttatagtct tggaacacca
     3121 cgccgatttc tctgcgtaga taaggaactt cgcggttttt gattgctatt agatcatgac
     3181 ctgcgacgtt caaagttcct tttgaagctt tttcttctcg atacatcaat ttgatgaatg
     3241 tagatttacc ggctccagac ggaccaacca catagacaaa ctctccttgt tcgatgacaa
     3301 tcgagagatt gcggatagcg gtcgtaccat ttgagtactt cttcattaca tccttcattt
     3361 caatcatggc gttctctcca tatctttttc agtctttagc tattatagca tggcaatgtt
     3421 tcaacatgct tgcaaaatct tacaatttta tttcaattta acaatgggtt cttattgtag
     3481 atgcattttt aaatagccgt caataaataa atcaaggtca ccgtccataa ctgcttggac
     3541 atttcctgtc tctacgtttg tccgatggtc ttttaccata gaatagggat ggaaaacata
     3601 agaacggatc tgtgaacccc agccgatttc tttttgttct cctcgaagtg cagctgcttc
     3661 ttgttctttc ttttccactt ctaattgata aagcttagct tttaacatcc ccatcgcttg
     3721 ctcacggttt ttcaattgtg agcgttgtgc ctgactagct actactgttc ctgtaggcaa
     3781 gtgagtgatc cgtacagcag attctgtttt attgatgtgc tgaccacctg caccacttgc
     3841 tcggtacgta tcgattttca aatcatctgg attgatttct atatcgatcg tttcatcaag
     3901 ctctggcatc acatctaccg aacaaaaaga agtatgacgt cgtttcgctg agtcaaaagg
     3961 cgaaatccgt acaaggcgat gaacaccttt ttctgatttt agataaccaa aagcgttatg
     4021 tcctttaatc agcagggtca cactcttgat ccctgcttca tcacctgctt gataatctag
     4081 tgtttctact tgatagccat gctgctctgc ccaacgtgta tacatacgta aaagcatgct
     4141 gccccagtct tgggactccg ttccgccagc ccctggatgc aattcgacga tcgcattgtt
     4201 tcgatcataa ggtccgtcta acagcataga taattcatac gtcttcattt tttcatctaa
     4261 tgcattgata cgtgtttctg cttcttcttg cattgctgca tcaggttctt cttgcagcat
     4321 ctccaccatc aattcgattt cttctagttc ttcggcaaga gaatggaatt ggtcataggt
     4381 ttctttatta gcgttatttt cgctgatcac tttttgtgcg gcttcgctgt catcccaaaa
     4441 accaggttca gccatttttg cttctgcttc cgcgatgtct tcttccagct gatctaagtc
     4501 aaagagacct cctgaaactt gtgacctttt cctgcatttc tgtcaggaga tttcgaattt
     4561 cggatatttc catgtttttt ccttctttca ctatactttt ttcattatat ctagtctaac
     4621 agaatcagca aataacagaa agaaggtgag acgaattctt aaaaaaagaa tttgtctcac
     4681 caatcctgct gtcagaaatt agtttgcatt ttttccatga cagttcttat attttttccc
     4741 gcttccacaa ggacaaggat catttcttcc gaccttattc acatggattg gctgtttttt
     4801 cgcgctagta ttactatttt ctttggcatc tccgtcttct gagtgagaag ctgttccttg
     4861 agaaacctgt tcacgctgta cgttttgacg gatctcagct ttcataaaga ttcgagtgac
     4921 ttcatactcg atggcgccta ccatatcttc aaacatctta tagccttctg tttgatattc
     4981 gactaatgga ttgttctgtc cataagcacg taagccgatt gactgacgta attgatccat
     5041 tgcatcaata tggtctgtcc atttagagtc cacaacacgc aagatcacga ctttttcaaa
     5101 ttccaacagt tgctctggac catttagttg agccgctttg gtattaaata cttcttgtgc
     5161 tcgttcgttt agatactctt tgatctcctg agctgttttt ccttcgatat ctgcaagagt
     5221 aatgctatct tcatggacaa gagtactgcc ggcaaaatcg atgatacttt ccaaattcca
     5281 attttctttc tctaattgtg tatgcgcatc tactacacga ctgattgtcc gtttgaccat
     5341 gttcattagt ggcttggata agcttttttc ttccatgatt acttcttgtc gctgtttata
     5401 gattacttca cgttgttcac gcatcacatc atcgtattgt aagacgtttt tacgtgtgtc
     5461 atagttattt ccttctacac gtttttgtgc agattctact tgtttcgtca gcatcttgct
     5521 ttggataaca gcatcttcct caccgatctt catccgatct aagaaagctt tgattcgttc
     5581 tgaaccgaat cgtttcatca aatcatcttc caatgacaag tagaactgtg acataccagg
     5641 gtctccttga cgtcctgcac gtcctcgaag ctggttatcg atacgacgag attcatgtcg
     5701 ttctgtcccg ataactgcta gaccaccaag ttctcttacc ccaagtccta acttgatatc
     5761 tgttccccga ccagccatgt tggtagcaat cgtcacagca cctttttgac cagcattcat
     5821 gatgatctct gcttctttaa agtgattttt cgcattcagc acttcatgag gaatacgttc
     5881 ttgattcagc atatctgata ataactcaga tgtttcaacc gcaacagttc cgactaacac
     5941 aggttgacct ttgcggtagc gatctttgat atcttgcacg acagcgtgga acttgctttg
     6001 taatgttggg taaagcaaat ctggacgatc atcacggata accggacgat ttgtcggaat
     6061 ctggtatact tggatgttat aaatttctct gaattcttct tcttctgttt tagctgtacc
     6121 agtcattccc gcaagtttct tgtacatacg gaaatagttc tggaaagtga tcgtcgccat
     6181 ggttttcgtt tcgtcttcga tctctacgcc ttctttagct tcgattgctt ggtgaagtcc
     6241 atctgaataa cggcgtccat ccatgatccg accagtgaac tggtcaacaa tcagtacttt
     6301 tccgtcttgg acaacataat cgatatctaa aagcatgata tagttggctc tcaatgcttg
     6361 atccaagtga tgggtcaatg cagtattttc aatgtcgtat aagttatcca aaccgaatgt
     6421 ttcttctgct ttttcgatcc ctgcttcggt taaactgatt gtttttgatt gtacatcgat
     6481 tttataatct tcttcttctt tcaaacgttt cacgaagttg tctgctcttg tatataaagc
     6541 tgtggatttt tctgcttgcc ctgaaatgat cagcggcgtt ctcgcttcat caatcaagat
     6601 tgagtcaact tcatcgacaa tggcataatt cagcggacgt tgtaccattt gatggcggta
     6661 aacgaccata ttgtctctca agtagtcaaa tcctaattca ttattcgtac tataagtgat
     6721 atcacaatta taagcctcgc gtttttcatc cgaagatttt gagttgatat tcaaccccac
     6781 tgttaatcct aagaaattat aaagttcgcc catttccgtc gagtcacggg ttgctaaata
     6841 ttcattgacg gtaactacgt gtaccccttc gccagtcaga gcatttagat atactggcat
     6901 cgttgcggtc aaggttttac cttcaccagt acgcatttca gggatatttc catcgtgaag
     6961 cacgattcca cccatcaatt gtacgtggta tggatataaa cctaatacac gttttgctgc
     7021 ttctcggacg actgcaaaag cttccggaag taattgatct aatgtttctc ctttttggta
     7081 tctgcccttg aattcagcag tttttgcctg taattgttca tcagttaatg ctgccatttg
     7141 gtcagcgtat gcttcaactt tttttgcaat gctatctaaa cgttttagtt ctttcttatc
     7201 gttttcgata agtgaacgaa tcaaattggc catgcgttgt gtaatctcct tttccttctg
     7261 tgtattatcc agactatgta gactttactt ttcattttat cattagttat agataattga
     7321 aatatcttaa gcgtattaaa gtaagaactt cataatttat tcatatttca tctaaaagag
     7381 gaggaaactc aaaaaaactt gcggcagctg tcagtagctg ctgcaagttt tcgttgttat
     7441 ttaagtaagg ctgatcagtc tgtttcaatc agtccgtatt ttccatcttt acggcggtaa
     7501 acgatacttg taccatttgt ttctgcatct tcaaaaataa agaagttatg tcccagcatg
     7561 ttcatttgta agactgcttc ttcactatcc attggtttca atgaaaggcg tttcgtacgt
     7621 acgatatcta attcagaatc attttcgttt tcgtcctctt cattgaataa agcagcagca
     7681 tctgcagtat tcatgcccgt ttcacgggat ttacgattaa tttttgtttt gaatttgcgg
     7741 atttggcgtt ccaatttgtc aacaaccaaa tcgatacttg cgtataaatc tggagaagtt
     7801 tcttcggcac gtaatacaag ataaggtaat gggatcgtta cttccacttt tgctgttttt
     7861 tctgtataaa cttttaagtt cacatgcgcc gttgcatcgg gtgcgtcact gaaatagcgt
     7921 tctagtttgc ctactttttt ctcaacgtag tcgcgaatcg cttcagttac ttcgatattt
     7981 tcgccgcgta cattatatct aaacataaaa attacccctt tcgtctacca caatcaagaa
     8041 ggagaaggac cactctttcc ctcttcttaa ttctattata tcgaactcta ggttatttgc
     8101 aaattaaatc gctatcaaat ttccacatct gattcatctt gccaaagaaa tggttcggat
     8161 gattttcggc tgtgcgttct gcaagcaatc tgctgcatga aaaagcgtct gtcctgttgt
     8221 atagacatcg tcaataatca atatttttt
//
LOCUS       DAYYGO010000116         7984 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000116 DAYYGO010000000
VERSION     DAYYGO010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.119"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            30..1667
                     /locus_tag="ACHA5T_002370"
     CDS             30..1667
                     /locus_tag="ACHA5T_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887916.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSMGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(1812..2552)
                     /gene="truA"
                     /locus_tag="ACHA5T_002371"
     CDS             complement(1812..2552)
                     /gene="truA"
                     /locus_tag="ACHA5T_002371"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFM8887917.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(2743..3378)
                     /locus_tag="ACHA5T_002372"
     CDS             complement(2743..3378)
                     /locus_tag="ACHA5T_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFM8887918.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(3525..4745)
                     /gene="sstT"
                     /locus_tag="ACHA5T_002373"
     CDS             complement(3525..4745)
                     /gene="sstT"
                     /locus_tag="ACHA5T_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFM8887919.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            5313..>7984
                     /locus_tag="ACHA5T_002374"
     CDS             5313..>7984
                     /locus_tag="ACHA5T_002374"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8887920.1"
                     /translation="MNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFICY
                     LLAVSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLFVHKSYFLVAAF
                     FYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLLLLISNRFW
                     YALTATLIINLLLTISTVIKMEMREEPVFPSDLKILTGLSELLSMVSPVLLIVGGLIL
                     LLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSPSFLLFNLF
                     KVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYNTKADEINQ
                     TRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLMLSVGYGGG
                     TANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHPFTAALYRR
                     KQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNTETSQFIQL
                     STMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKFIQEIDKID
                     KPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNKEVVAPNNF
                     GAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTEDSQIIQEK
                     NLTEKQKEILEDYRLIQYDLVAGNQYSATWATQK"
BASE COUNT         2614 a         1493 c         1238 g         2639 t
ORIGIN      
        1 tttttattac ttttaaatag gagaaaaaaa tgaaaaaaaa caaaaaccaa ataatagata
       61 tcatttttat gttatttcta agttttgctt acgtcatccc tctattcatg tctatgggaa
      121 tctatcatag tgtcaatcag gatacttact ttcatttatc tcgaatcata ggactagata
      181 atgtatggag ttctccagta aacttcaata acttcgatca tcatggaaca atgatgaata
      241 ttttttatcc atggcttact ctttatccag cttttttatt ttataaaatg atgggaaatc
      301 ttgttttaag ttataatatc tactattttt ttatcacttt tttaacaatg gtcgtttctt
      361 atttttctat gaaacaaata aaaaacaatc gttatatttc gctgttattt tcgattattt
      421 atacattttc cgcctatcgt gccattgaca tattccatcg tgcttcattg ggagaagctg
      481 tcgctttaac atttttgcct ttgatactaa tggggtgtta cgaaatttat attagagatt
      541 accaaaaatg gtactggttg tctattggca tgacgctagt agtgtatact catttacttt
      601 ctgtcgctat ggtttctgta tttattggag gaacgttgtt tctttctttt tatttttggg
      661 accaaaaaat cgctagactc ttaagtcttt taaaagctac cgtcctcacc ttttttttaa
      721 gtgcgggatt cttaattcct tttattcaac aatctagagc acaagaattg aaagtcccac
      781 tgggaaaaga attaagtggt atggctccta gtgatatgct gacacacatt ttaaataata
      841 actataacaa ctatactatt ggattattct tatttctcgg attgattggt gcttttattt
      901 ttataaaaaa attaactgct gatgatttat ttattttctt tcttggtgta tttgttcttt
      961 tttgttcaac caatttattt ccttggcaac tttttaacca tacaccagtt aagagtctac
     1021 aatttgtctg gcggttaaat ggtttttctt ctttatttat agcctatacg atgagtatag
     1081 tgatctatta tattttttct actaaaaata attcatggaa aataatggtc tttacgtttc
     1141 ttgcactaat attgcatctc tcaggagtaa gcaattctat ccatgctaac aaagattcat
     1201 taacacttat agcacctgaa gacgctgttg ctatagctga aaattacaat catactgatt
     1261 atgcaaacaa agagtcaatc tatcatcctg atatgattaa taatgatcaa tatttactag
     1321 gtaaccagga aataaatcca acaactcatt ttatgagtaa caaacttaca attgaattag
     1381 ataattcgaa taataaaaat actgtcttga cgacacctat ttatcgttat aaagggcaag
     1441 tggcttctat taatggcaaa ctagtacaaa caaaattatc taagtttggc acaactgaat
     1501 taactattcc tccaggtata aataaagtcg ttattactta tcagtacaca aaactagcaa
     1561 ttgcttcaag atatttatct atcgttactt tgattttatt tttactttat cgatttactt
     1621 tttctaaaca aaaacaaccg agaagagaag tacaacattc tcactagatt tttatttaag
     1681 aaagactttt tagctgtaca cataaacagg ctaaaaagtc tttttttata tttctttaat
     1741 ttgcttcata cattatttaa catcgatatt tatactgtga aacatgcgtt tcatccatta
     1801 tcaaagtatt tttatcgata ataaacttca tccaaaaaca gaccttgtgc agggacggtt
     1861 tctcctgcat gttgccgcac tttgctttca aatatttcat cgattgtttc tatcggcatg
     1921 gcacctgtgc cgatttctag taatgtcccc atgataatcc gcaccatttt atataagaat
     1981 ccgtctccga caaaagtaaa atgaagcatt tctccctttc gttcgataga taattcatca
     2041 atcgtacgca ctgtggattt ttttgatttt ttcaatgcag aaaaaccaat gaaatcatgt
     2101 gtacccacta atttttcgca agctgcattc agcttgtcca tatccaactc ttgagggaaa
     2161 taaaagctgt ggtttcgctc aaatgctgaa ggaatcacgt tgttccaaac ataatagctg
     2221 tattttttgc ccaccgcatt atatcttgaa tggaagcgct caggcatttc ttcgattctt
     2281 ttgatgacaa tatcccgcgg aaggtaacga ttcataaagt cgatcatttc tgccagtcca
     2341 atttctgtgt ttgttttaaa atttgctact tgtcctcgcg catgggtgcc agcatctgtt
     2401 cttcctgatc caataatttc tatctcttca tttgtcatct gatgcaatac tttttcgatt
     2461 tttccttgaa tggttttatc gtcatctcca agtctttgcc atcctgaata tctttttccg
     2521 tcatattcga ttgtcaactt gatgtttctc atattttagt ggctcctctg tcttccattt
     2581 ggtcttctct ctaccttttt aagattgtag cacaaaatat agctcctttc ttctctaaaa
     2641 cagaagctat acaagaaaac tgtggaaata tatatttcag aaaaacaaaa aacagccacc
     2701 ggatgaagta tccgctgact gttcaatttg acggctcaag ttttactgtt tgagatgcaa
     2761 gatactcaac caggcaatgg gcacataatt aaagaaaaaa ctgattcttt ggcaaaaacc
     2821 tattttattc agtaatggcc aagaatttat agaaggaatt cttgccagtc cataaatcgc
     2881 cgcaaaaagc aaactgatca cagacagtcc aaggtataag tggcttacgt ccatttgccc
     2941 attttttcga tagatcaaga aaagaagaac tggaaacaat aaaaaaccac tgtaacctaa
     3001 acctgatccg atattatgga cccatgtcga gacattccac gaagattcat gggtattgat
     3061 actgaacaaa cctgtaaaga tacaatcacc tataccatag aacgcgatgg ctgctgtaac
     3121 tagggtagct aacacacgag atgtacttac aaatgcagca tacaatgcag gcaatgacag
     3181 gatgaaaaag atccctgaaa ccacagacca aataagaaac gcttttctta caggactgga
     3241 aacctctcca aatacactga tcacgctctt catttgatct aattcaggat aatacaatcc
     3301 taatacgtac ggagtcaaaa aatcactcag cacgcctaat aataaaaaga aaaaaccaaa
     3361 tttacgtaaa aaatccatga tacgcctcca ataacaatta cctatgcttt taattgttat
     3421 tataagcgac tttatcttga tttaacagaa acctctattt acacgaacat ctatataact
     3481 cattaaaaac tcgaatcatt ttaaaaatac taaatatcta acgttcaacg ttcacttaat
     3541 ccatctaaac aatctttcaa tttgattttt tccccacttc ttttacgcgc gcctagctct
     3601 cctattgcag taaacaacag atcacttgac gaatttaagg cagtttccat tgaatcctgg
     3661 ataacaccga taataaatcc aataccgacg atttgcatcg cgacatcatt agagatacca
     3721 aacaaactac atgctaatgg aatcaataac agtgaaccgc ccgcaacacc tgaagctcca
     3781 caagcagcta aagcagacaa aatacataaa aggactttca aaacaaaagg tgcttcaatc
     3841 cctaaagaat gtacagcagt caacgtcatc aaggaaatgg taatcgcggc accgcccata
     3901 ttgatcgtag cacctaacgg gatcgatatg gaataagact cttttgtcaa tttcaatctt
     3961 tcagccaaca tcatgtttat cgggatattt gcggcagaac ttcgggtaaa gaaagcggga
     4021 agtgcgcttt ctttcaaaca aaagaaaatc aatggatagg gattttcttt aataaacaaa
     4081 aacgtgagga atgggtacac aactaatgcg acaaacagca tcgtaccgat caaaacaagt
     4141 aaaagctgca gatactctgc caatccagct attccagtcg tagcaattga ttgataaacc
     4201 aatcctaaaa tccctatcgg agcaaatgca ataatcactt gtacgacttg tgacaacgct
     4261 atagatatct gatccaccaa ctcttttgtc gattctgaac gtgctcgaaa agctagtccc
     4321 aacaatacag cccagaataa gacgccaagg taattcgcat caataagaga ttgaacagga
     4381 ttttgtgtga cattcgtcaa catagtagat aaaacacttt ccaaactttg cggtgctttt
     4441 tcagatactg tttcttgtaa agtaatagga acagtaaaaa ttgaacttac aataactgct
     4501 acaagtgcag aaagcaatgt tcccactaaa tataagatca aaataggctt cacgaatgtc
     4561 ttattcccaa ttttatgctt ggcaattgac gccatcgtca agaaaaaaac cagaataggt
     4621 gcgattgcct tcaagcaact gacaaacaac gttcccaata ttgaaataaa tgtccatgtg
     4681 gggaaaaaga taccaaacaa tattccgact aaaataccaa caacaatacg ttgaactaac
     4741 gacatatcca aaaacttctc taacaaatga ccaccctttc acaattttaa caatcattgt
     4801 atcattttat gcaaagaaac taaaggtata attttctaaa aattctaaca atacaaacaa
     4861 ccatttcttg ctaattttgt actttttctg tctacacgtt gttttttgag atattctaag
     4921 ttaggatgtt attaatgggc aacttatcag gaaacgtttt cttatgtttt tgtccaattt
     4981 ttttgtgagg tgtttattct tgaaaaaaat aaagaaggag agttatggct tcctgacaat
     5041 ctttgtcgct tgtataatga tggttttaca gcgtatattg atttcagcag tcactattcc
     5101 tacatatgca acaaaccctg gtcgattcta catctataca tttttacaaa cttccgttgt
     5161 tattggtgca aatttgatcc cactttacat gggttaccaa tatcaaaaaa tcgcccgttt
     5221 aaaagtttta cattatttaa gtcgttttgc ctttatattc ctaatagcca cactagtttg
     5281 caatatcttt tttttacgta caagctggca gcttgaatat tcgtgattat tggcttatcc
     5341 taacacctat cagtcagaat tactttactt atgctgtttc ttgtactttg cttttttgta
     5401 gtattcctta tacagttgga tttttaaata aattatcgaa aacaacaatt aaatatctac
     5461 tattattttt aacaattgga ttagttggct gctctactct atttaataaa gacccgtttg
     5521 cattacagaa tggacagagt attttatgga ttttttattt atttttaatg ggttatggtt
     5581 tgaaagaatg gaactggaaa gaaaaggtcc gctataagct tcctcaagta tttatcagct
     5641 tgattgtttt attcggtttg attattttga tgacacaaat ttcactcatc atcagaggaa
     5701 atacagatac agcgctgagg ttttctaaac catattctct cttcgcaatg tattatactt
     5761 tctcttcgtt tttattttta gaaatgctat ctgataaatt aggtataaaa ggaagaggaa
     5821 attttcttgc tgtatttttg attgtcactc aagttatatg taatttccct ttagtttctt
     5881 atcgcgtatc tacatttatg aaacgtgaat ttccaaactc agggaaggca tggctgataa
     5941 atatcgggca gttttttatt tgctatttat tagcagtgtc cctcctagtt atctttttat
     6001 tttatataca aaaaatccgt ttcattcatc gtttcatcga atacttcgct tttgattcga
     6061 ttgaacaatt aactcagcgt tggaaaaaaa gaattcattg gctttttgtt cataaaagtt
     6121 attttttagt tgcagcattt ttttactgct ttaccttcgt gcagattttc gttttagaac
     6181 caaaagaagg ttggaaagaa actatccagg ttgccttaaa aatatttact cagcgacaag
     6241 cacccatgat cctgacaatc atcattatta tgggattttt cttcttacta ttgttgattt
     6301 ccaatcgttt ttggtacgcc ttgactgcta ccttgatcat taacttactg ctcactattt
     6361 cgacagtaat caaaatggaa atgcgggaag agcctgtctt tccttcagat ttaaaaatac
     6421 tgaccggatt gtcagaacta ttatcgatgg ttagcccagt cttattaatc gtgggcggat
     6481 tgatccttct cctattattg attactagta ttattgtaca acgtagacta caaaaacagt
     6541 attccttgaa gattcattgg aaacgtcgat ttatcagtat cgctgtatta ttaggatgtt
     6601 tttcgggtgt ctttttcatt aatcacaaaa attcgccttc tttcttgctg tttaatttat
     6661 ttaaagtaaa caagactttc tttaaccagc aaggcgccgt aaaagataac ggaccagtca
     6721 ttcaattcct aaacaatatc gacattaaaa tcatggaaga acctgctggc tattcgaaag
     6781 aaaaaattaa tggaataatg aaaaagtaca acacaaaggc agatgaaatc aaccaaacaa
     6841 gaagtgactg gttaaacaat cagacgatcg ttctcaattt aagtgaaagc ttcagtgatc
     6901 ctgcacgtgt ccctaatctg acagtaccta cagaccctat tccttacatc aaaagcatta
     6961 taacagaagg aacaggagga ttgatgttat ccgtcggtta tggtggcgga acagctaaca
     7021 tggaatggga aggactgaca ggcttgagta tcagtaacct gtctgcttcg ttagttacac
     7081 cttataccca gctagtggag cgtcagaaaa taagtccaaa tattaccgat ctattcgatg
     7141 aaaaaatagc catccatcct tttacagctg ctctttatcg cagaaaacaa gtgtttgata
     7201 aatttggttt tgacaggttc tattatttag acagtccgga taaattaact tatacggata
     7261 aaatagaaca aagcccacgc atatcggatg attcagcata caaagaaacc ttgaaagctt
     7321 tacaaagcaa tacagaaacg tcacaattca tccaattatc cactatgcaa aaccatatgc
     7381 cttatgatga ttactatagt gaattgaact acactgctga aggaactgct gtcattccta
     7441 atagaagaaa agaattacaa acttatatgc aaggccttca ctataccgat acagcagtaa
     7501 aaaaattcat ccaagaaatc gacaaaatcg ataaaccgat tacctttgtt ttttatggtg
     7561 accatttacc cgcaatctat agtgggaatg attcgaaaaa atatggatta gaacagcacc
     7621 agacagatta tttcatttat aacaacaagt atagtcgtga acaagcagct acgttaaata
     7681 aagaagttgt tgctcctaat aactttggtg ctatggctct tgagcatgcg aatatcaaag
     7741 taactcctta ctatgcgttg ttgacagaag tatctaatca tctacctgca ttaacaattg
     7801 atcctacaga aagcttgtct aatcgtttta atggaaaaca ggtctttgtt acagaggata
     7861 gtcaaattat ccaagaaaaa aatttaactg agaaacaaaa agaaattcta gaagattatc
     7921 gtctgataca atatgatctt gttgcaggta accagtacag tgcaacatgg gcgacacaaa
     7981 aaat
//
LOCUS       DAYYGO010000117         7839 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000117 DAYYGO010000000
VERSION     DAYYGO010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7839
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.120"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(69..1490)
                     /gene="tyrP"
                     /locus_tag="ACHA5T_002375"
     CDS             complement(69..1490)
                     /gene="tyrP"
                     /locus_tag="ACHA5T_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFM8887921.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(1733..3610)
                     /gene="tdc"
                     /locus_tag="ACHA5T_002376"
     CDS             complement(1733..3610)
                     /gene="tdc"
                     /locus_tag="ACHA5T_002376"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFM8887922.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(3901..5157)
                     /gene="tyrS"
                     /locus_tag="ACHA5T_002377"
     CDS             complement(3901..5157)
                     /gene="tyrS"
                     /locus_tag="ACHA5T_002377"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFM8887923.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(5269..5474)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5737..7038
                     /locus_tag="ACHA5T_002378"
     CDS             5737..7038
                     /locus_tag="ACHA5T_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFM8887924.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(7107..7706)
                     /locus_tag="ACHA5T_002379"
     CDS             complement(7107..7706)
                     /locus_tag="ACHA5T_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8887925.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT         2327 a         1599 c         1372 g         2541 t
ORIGIN      
        1 aaaactattc acttggcagg acctcctact aaatcagcga caaaacgtcg gctgaaagac
       61 ttattttttt atttttcttc taaacttttt tctacctcaa tcccccaaga atctttcttg
      121 aagtgattga tgatcaatgg aataacgaac atgaccactg tgatagctgc tactaataat
      181 gcatcactca atgtcgctgt acctaatgta gcaaatacga caacgatgat agagaagatc
      241 agcatgcagc tgactaccca agctaatccg tttccgtttt taccaattcg gtaaggacgt
      301 tcgaattctg gatgtttctt acgcatttta agcaatgaaa cagcaatcaa aatgtacacg
      361 atacaataaa taacagttgt agcatttgta agtgtcaaga atacagcact tacgtcatcc
      421 attacaccat aaagcaaagc aaataatgaa ataacgattg attgtgttag aacgacattt
      481 ctagatacgc cgtatttgtt ttctttatgg aatccgaatt ttggtggaag gaatccttca
      541 cgagctactt ggatgattgt ttttgatggt ccagtaaccc aagctgataa ttgtaaaagc
      601 acaccaataa ataccatgaa gctgaagata tttccgatca cttctggtaa gcctaggatt
      661 tgacagtcaa tcaagatagg ctgcgtgata tttgctaatt ccatttttcc atctggtacg
      721 acatttgcta caagcatcgc attgatgaca ttcaataaaa ctaatccgat caaagcgata
      781 aatacgcctt ttgtataatt ttttgttgcg tctttcaaac gaggcatgta aacagaactc
      841 atttcgatcc caacaaaaat aaatgtgata ccagctaagt atttcaatga atcaatatgt
      901 tccaagtcag ggaaagcttt tgaccatgaa aagtcaccta gatagccgtt tgagactagt
      961 ccgactttga aggctgccaa aacacctaaa acgaacatga tgactactgg gatatatacc
     1021 cctagccata caccgatgtt cccgccgact tttgccatat cgaatttcaa gttcaagatc
     1081 gtgatgatcc agtagataac taaaatacaa cctaaaacga accagtgatt attgccaagt
     1141 tctacattac caaatgtgtt tcctaacaat ggtcctaaag tagaagcgac cattaccata
     1201 cctgggaaca tttgcaccca taatagccat gcaacgacaa atccccattt gctgcccagt
     1261 gctgttttta cccataactg aggaccgcct tcttgaggaa gcatcgtgga aagctcaccg
     1321 gaaaccattg atatcggacc ggcaaaaaac aaaactgcaa aaattgaata aaaaatcaat
     1381 gtccaaccga ctgcggctaa cgtaggaata ctccgaaccg ttgcacacag tgccattgtc
     1441 agaccgataa aggtcccaag acccatttta cttacttcag atttactcac cgttgacact
     1501 ctccttgatt ttttggataa aataacggtg tctttgaagt agtttcagca ataaacgaaa
     1561 caattcagcg aatatttgag gggattcttt gagtttattg cttgaagggt ttcttcaaag
     1621 acaacctcaa aacaaaggga tcgaagagct taggtctccc cttgctcttc agatccaaac
     1681 atgcgtcaga aacagttagt attcaagctg ttttctaaca tgattacctt gcttattttg
     1741 cttcgcttgc caataattgg tctgcgtaga ttttttccaa tttttcttga agagctgctt
     1801 tgattttttc agcatattct tcaaagtgtt cttctttgtt catgtatggt gtcattacgc
     1861 tggcacgcaa tacagttact tttccggcac ggttccattc ttcatctgag aatcctagtt
     1921 ggttcacaaa ttgtaatggg ctgttgccat agtctgggat agcaaagtca gtatgtgaag
     1981 ttaagaactc attaccgtaa atgcttcctt tcacgtatga agaataatca tatacgtcat
     2041 ggttcaattt attcattgca actaagtcat cgttgccttt ttctttgaat acatagtcta
     2101 ccatattgaa atctggataa gttaatggat gaacttcgat ttctttgtct cctactttaa
     2161 atgacaagtc attcaagaag ttgtagaaac gatgtgcacc ttcgattgat gcacccatta
     2221 atttaccata tcctgtgaca ttcaaaggta agacatggtg tgctgcccaa acgctggctg
     2281 ctgttgcccc tgcttttgaa ccttccaaga tataagcccc aagtaatgct gggatatctg
     2341 cgcctttttc aaatacatat gtcgcaaagt aagagataac gtctctcatg cggatatctt
     2401 tgatgacgat accaccggca gaatatggaa catagcccat tttatgtggg tcaatcgtta
     2461 cagattctgc ttcttcgatt gctttatatg cactgtgtac ttcttctaag atataatctt
     2521 tgttttctgt aaagacattg tatttgtaat gaacatcttt taaatcttca aatggaatga
     2581 agttgttgtc ttcgtctaag aaaatcgcac ggccgtatcc gccataagca gcatctacgt
     2641 gtaagtagaa gtaaataccg tctttttcta atacgcgacg caatgcaacg attttatcga
     2701 taccgtcgat tgctccttct tctgttgatc ctacaacacc gactacacct aggattggtg
     2761 ttttttcagc tgctagacca cgaacgattt tttcaagttc gttgatgtcc atgcgatagt
     2821 tgtggtcaac tggcactgga atcacttggt ctaaaccgat accgatgatg tcggcagctt
     2881 ttagccatga atagtgttta gtttgtggaa ccaaccattt tcctaatttt tctaaatgtt
     2941 ttccgctacg tgctgagtga gctttgattt cgtcgatttt ttctgggacg ctgtctaaca
     3001 gattcatgat ttcttctgtt gacaagttca ttaattccca atcgcttttt ccagcaacta
     3061 gttcaggtgt cacttctttc attgcaagcg gcaatgattt gatgttgcgg gcataccaaa
     3121 gtccttctaa gtttgctaaa gaaccatcag caacgatgtg gccccagcca tctttatagc
     3181 tcattaattt tgcaaattcc attcctactt cttcttccat ttggcttgtt gcaggagaag
     3241 attcataggc aacgttatta ccattccata gcatagcaaa attgtacgct agtaaagatg
     3301 gcatcaatgt ttcagagttc atatgacccc agtaacgtcc ggcattatgc caaggaacgg
     3361 aatgggtacg catccgcgct gaaatttctg atagaacatc ctttgtttta ttcacagtgt
     3421 gttcaaaact agcactgctt ttttcttctg gcgtaataat aggcatgtct tgaggcatgt
     3481 agttttgacg ccagcctaaa tgttcatcca ccaattcatt caacaaagct ttatagattt
     3541 ggccgttctc agctttgtcc ccaatgaata gggcatttag gtttagatct ttcgacaatg
     3601 attcactcat agtgaaaccc tcctaaaaaa atatttaaat tttgtcgagc cctacattac
     3661 acctgttctt ctattctgta aactaagttt tggcctgatt tttaatcata ttttaatatg
     3721 tatcaattat ccgaattgtt cacacttttg aaataattaa aaaaaacgag ggaatttaca
     3781 aatgtaaact ccctcgcaaa gattgtatta acgacaacca gcgttctatt ttaggatggg
     3841 tttcaaaatt ggaatgttgt aatagtattt ccctttttca aggtaaaaac ttactaaatt
     3901 ctaatcaatt actttggcca aaaagtaatt tttcttacct tttcgaataa ccacaaactt
     3961 accatcgaat tcatcggatg gatttatgac aaaatttaaa tcggtaacgc ggtcaccgtt
     4021 aatacttata gctccgtttg aaacatcttc acgtgcttga cgtttagatg gttcaatttt
     4081 tgtggaaaca agcagttcca cgatattttc aggtgtgctt gaaatttcaa cgttcggcat
     4141 tttaccgaat ccttgcgcga tttcttccgc atttagttct ttaatatttc cagagaacaa
     4201 tgcttgcgtt attttttgcg cttctttcaa gtcttcttca ctatgcacaa atcttgtaac
     4261 ttcttctgcc aaacggcgtt gtgcttcacg tttctcaggt tctgtctcta cttttttcgc
     4321 taagtcttcg atttcttctt gagacaagaa agtaaagaat ttcaagtatt tcactacatc
     4381 gcgatcatct tgattcaacc agaattggta gaactcaaat ggtgaagttt tcttcggatc
     4441 aagccaaacg gcaccgcctg cagtttttcc gaatttcgtt ccgtcagcct tcagcatcaa
     4501 tgggatcgtt aaaccgaacg cttctgcttc tggtccttct aattttcgga ttaggtctaa
     4561 tccagctgtg atatttcccc attggtctgc tccaccgatt tgcaattgca catcgtatgt
     4621 tttatgcaga tgcaaaaagt cgatagattg caggatttgg taagtaaact ctgtaaatga
     4681 gattcctact tccaaacggc tggcaacgat atcttttgct aacatcgtgt tgatgttgaa
     4741 gtttttaccg tagtctctca aaaattcaag taaagagatt tttgacaacc agtcgtagtt
     4801 gttcacaaat gtgatatttg catctttacc aaacaatttt ctcatttgat tggaaagtgc
     4861 gtccacgtta tgttgcactt gttccatcgt ttgcaacaca cgttctgttt tgcggccgct
     4921 tgggtcgccg atcgttcctg ttccgccgcc aatcagaata tatggatgat ggcctgccaa
     4981 ttggaatctt ttcatcatca taaatggaat caaatgtccg atatgcatcg aatcaccagt
     5041 tggatcaacg ccgcaataaa gtgaaatgct cttttcttca acgagctcgc gaagtctttc
     5101 ttcgtttgtt tgctgattaa tggcgtcgcg ccaagttaat tcatcaataa tattcatttt
     5161 actgacctcc gtgtagtttt tttatgaaag caggaacctg ctagcatgtg taattaatta
     5221 tttcttcagg ataggatagg aaataaaaaa agtcccttgg catataagcc aagggacgaa
     5281 atctcgcggt accacccaat ttgtatgtct agatatccaa catacctctc gaacatttaa
     5341 cgctgtcgcg tggcaatcac tgccaaagac tcctgaaatg taattcgctt taaagcatgt
     5401 actggtttgc agcgaccacc agctttctga aacattggct ttaacaacta cttcgtttcg
     5461 atcttcgtct tgttattatt aatatgtgtc tatcatagag cgcaactttt tctttgtcaa
     5521 cagaaaaagg atattttttt taaattcctc attttttctt ttttgaatcc gctctaattc
     5581 cgaacatttt attttcaatt attgttaaaa gaaataactt tttctgtatt ttttctgcaa
     5641 tttatcgaga agtcacttta ctttgcctct tattctttct tcgttacaat aaggaatggg
     5701 acaagtaaaa aaatgaaaaa gcaagggacg aggtatatgg aaaaaaagca aaaaattatt
     5761 ttaggagcca cttctgcttt aactatcgtt attttaattc taagtttgtt ttttgtcatc
     5821 cgatcatcta caaaaaaaga accaatcatt cctgaaaaaa aaataacaga aaaagcaaaa
     5881 gaaccgacag atgataagca acaactagtt actcaagcaa aaaatctggc tcaaagctac
     5941 gattatgacc aagcgattgc gttattggaa aaagatagta agaacggatc tactgaaaca
     6001 caacagcttc ttcaaacact aaaaaaggag aaagaacaac tgatcgactg gtctgatcct
     6061 actcagatct ctcatgtctt tttccacagc ttgatcgtgg acccgcaaaa agccttccac
     6121 tctcaacagg cacaaggata caaagattat atggtgactg ttgaagaatt caaccgttcg
     6181 atcgaacaac tttatcaaaa tgattttgtt ttagttaatt tgaatgattt gatccaaaaa
     6241 gacgaacaag gaaatctttc ctttaacggg cttgcgctac ctgaaggaaa aaaaccattg
     6301 gttctttcac aagatgatgt cagctattat gaatatatgg ataattccgg ttttcctagc
     6361 cgattggtcg tatcaaataa caatgaaatc aaaaatgtct ataaagagaa agataaagaa
     6421 gaaatcggtg actttgacat ggttcctctg atcgatgcct ttgtcaagaa acatcctgat
     6481 ttttcttatc gaggcgcaaa aggtaccttg gccctgacag gttacaacgg agttttaggt
     6541 tatcgaacat caaaatctca atatggagac aatgaaaaaa caaaaaaaga gattaaagaa
     6601 gctaaaaaag tggcggaaca gttgaagaaa gacggctgga gctttgcttc ccatacatgg
     6661 ggtcacttga atatgactca agcttcacta gaagatgtaa aaaaagacaa tgaattgtgg
     6721 cagcaagaag ttgcacctat cctaggaaaa acaaatattc tgatttatcc atttggtgca
     6781 gacatcagcg actggcagcc ttattcagca gaaaatcaaa aatttgctta cttgaagagt
     6841 caaggtttcg atattttctg taatgtcgat gcttctacac cagcttgggg acaattaggt
     6901 acggatttct atcgaaatgc tcggatcaat atcgatggca ttcgttttca atctgattta
     6961 gacggaaaaa atcctattct tgatccattc atcaatgtcc atgatgtcta tgacaaagaa
     7021 gcacgcgaat ctgcttgatt ttacgtcatt tatgaaccaa aaaagccgaa ctgacatttt
     7081 atcgtcagtt tcggcttttt tatgctttat ttcttcttcg gtgcgattcc cttgatcagg
     7141 acttctgtcc attctgctgt atatggaagc agttttgcgt atatctccgg cttagaaatt
     7201 ccttctacca ttgcttgaaa atagaggatc aagaactcat ccgaatattt caaatccact
     7261 tttccttctt tccgccctcg atggaaaaga tcaagcatga tactgaaact ttcttgcgta
     7321 tacgcatcca tcgcgtcaga aaattctttt ttttcttttt ttgtatagta tgtcatcaaa
     7381 tcgatataaa attgtttgtt cactcgattg agataacgga ttttgttttc actcatggca
     7441 atcagtgttt cttcaaatgg aagattttcc gcaatcaacg ctttcgcttc ttgccctaaa
     7501 tcattcataa atgcaaaaaa tacttcataa atcagccctt ccttgctgcc aaaatattta
     7561 aaaatcgtcg ctttacttac ctgagcgttc tcagctacct tatccattgt cactccagcg
     7621 gttcctatgt ctgtgttcat caagtcaaaa gctgcttgta aaatctcttt ctttttctct
     7681 tgtgctctct tttcaaaacc gttcatttgt agagcctccc gtaattttaa acttttataa
     7741 tatatatagt tcaaatttat tctgaggttt attataacta tctattgaga aagtatcaag
     7801 ctagcaattc cttgtttatg aattcattga cagaaattt
//
LOCUS       DAYYGO010000118         7760 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000118 DAYYGO010000000
VERSION     DAYYGO010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.121"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(552..1898)
                     /gene="gor"
                     /locus_tag="ACHA5T_002380"
     CDS             complement(552..1898)
                     /gene="gor"
                     /locus_tag="ACHA5T_002380"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFM8887926.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(2032..3285)
                     /locus_tag="ACHA5T_002381"
     CDS             complement(2032..3285)
                     /locus_tag="ACHA5T_002381"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFM8887927.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(3291..4214)
                     /locus_tag="ACHA5T_002382"
     CDS             complement(3291..4214)
                     /locus_tag="ACHA5T_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFM8887928.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(4435..6927)
                     /locus_tag="ACHA5T_002383"
     CDS             complement(4435..6927)
                     /locus_tag="ACHA5T_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFM8887929.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(6934..7401)
                     /locus_tag="ACHA5T_002384"
     CDS             complement(6934..7401)
                     /locus_tag="ACHA5T_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFM8887930.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(7537..7610)
                     /locus_tag="ACHA5T_002385"
     tRNA            complement(7537..7610)
                     /locus_tag="ACHA5T_002385"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7574..7576),aa:Arg,seq:acg)
     gene            complement(7668..7741)
                     /locus_tag="ACHA5T_002386"
     tRNA            complement(7668..7741)
                     /locus_tag="ACHA5T_002386"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7705..7707),aa:Pro,seq:tgg)
BASE COUNT         2161 a         1691 c         1247 g         2661 t
ORIGIN      
        1 aaatcagtgt tttgtcctga gtattagaga aacaaatgga aaaatagttc ctcatatttt
       61 gccgtttttt gagctaatac cgaaggacag ctgattgaac actatttgtt cggaaaaagt
      121 gactaactat gacacaattt ttttcacagt cccttttttc acttgatttg attcgtacca
      181 gcgaacttga cgagaacttt cttggtttgg taactatatg gtattctttt aacatcctcg
      241 tttgcttctt ctaatttatt tatacaaagt cacacttttt taaacatctg ttcttcgata
      301 catttctatt tcttttaaac atcgatacca atattttgaa cttaagagtg cataccaaat
      361 ggattcattt actcttcgtc agttacgaag ataatcaaaa tctccaactt ccctacttat
      421 ttcgttttga gaattatcga agcaatctta cataaataac taaaaaacaa aaaaccagaa
      481 aatcagtata ctgattttct ggttttacat ggtttgttca ataaaaagct gttggaactg
      541 ttaatattaa attatcgcat cgttacgaat tcttccgctc ctgttggatg gatggctact
      601 gtattgtcaa agtcttcttt agttgcaccc atcttgatag ccacggcaaa accttgcagc
      661 atctcatcga caccaacacc aatagcatgt aagccgacga ttctttcttc ttttccgaca
      721 cagatcaatt tcatttcgca tttttggcga taatcattta atgcaaagta cattggggta
      781 aagcgtgaac gataaacttt caattcattt tcgccatatt tttccaatgc ttcttcttct
      841 gtcaaaccaa ttgttgcaat tggtggatgc gtaaagacga ctgtcggaac aagtttatag
      901 tcaagataag aatctttctt tccattaaat aaacgttctg ataaacgtct accagctgcg
      961 attgcaacag gagtcagatc taatttaccg atcacatcac caacagcaaa aatatgttca
     1021 tccgttgtat tttggtattt atctactttg ataaaccctt ttttgtccaa ttccacattt
     1081 gtattttcca gacctaattt ttcggtattc ggtacacgtc caccggcaaa aagtacacaa
     1141 tcaccagtta aggtttcacc attttcaaat agcaccgtat attcgtcatt gtttttagta
     1201 atttctttag caatatgatg tgcgtaagtt tttattccag cttcttggta catttggatc
     1261 aaattgtccg aaagcatttt atcaaacgtt ctaagtggtc gttcttttcg atatgcccac
     1321 atgacttcac tacctaaacc acgaaataca cctgaaagtt cagccgcaat gtatccagca
     1381 cctaaaacga ttgctctctt aggtaactct gttaattcaa aaaatccatt tgaatcaatt
     1441 gcgtattctc caccaggaat ggtcatttta cttgcacggc ctccagtagc gatcaagatt
     1501 ttaggagcac gataggtttc accgtttact tcgatcgttt gactatcgat aaaagtagcg
     1561 taacctttta tagcctcgac atgattgcta tctaatcctc tttgataagc accgtgtaga
     1621 aaatcaatat atttttctcg tctttctact agaccggcga aatcaacatt ttttaaatct
     1681 gcttggatac cgtaacttga ggcatctcgt tcaattgttt ccaaaatcgt acttgcttgc
     1741 cacataactt ttttaggtac acaaccgacg ttgacacatg ttccgcccaa ttcattcgct
     1801 tcaatcagca aaacttttgc gccatgcatc cctgctctgt ttgctgaagc aataccgcca
     1861 gagccgccac caattacgat gtaatcatat atttgcattt tgttcctcct tattgaagag
     1921 atcaatcaaa acctttattt aacgatcctt tttctacaga aaacaagtgt aacacttttc
     1981 ttgaattttg tatgcgaaga tgcttaaaac agggatatat cagctttttc cttatttacg
     2041 ttttcggatc atatctccag gtttcaccgg ctgtgcgact ggcatagtta aaatcatcat
     2101 tggatttggt gctcggtcaa tggattcgcc ttcttcatta tgcataactt caacggtttg
     2161 actgaaatga ctgaatcctg gaccataaaa ttcgatttca tctcctacag aaaacaagtt
     2221 tctctgacgt atcgtagcaa tctgtgttgc ctcatcataa gataatactt caccgacaaa
     2281 tttatatact gggattttac gacgttcacc gaataattgt tcgttttcag ttggtgtatt
     2341 gtagtaaaaa cctgtagcta attctcgttg tgctactttc cataactcgt cgatccattc
     2401 ttgcttgcag acatagtttt caggatcttc catataactg tctacagccg ctttatagac
     2461 atttgacacc gtagatacat aatggatcga tttcattctt ccttcgatct tgaagctgtc
     2521 tacgccgttt tcaatcaatt ctggaatatg ttcgatcatt gacatatcca ctgcactcat
     2581 cgagaattct tcttccactt cgcctgtatc agttaaactg tttcgttctt gaccaaaagg
     2641 catatcgaac aatccatact tccagcgaca agattgagaa catccgccac ggtttgcatc
     2701 ccgcatcgac atatggttgg acaatgtgca tcgaccagaa taagagatac acatggcacc
     2761 atggataaat gcttcgattt cggtatccgt atttttacga atctctgcca cttcctccat
     2821 tgaaacttca cgagctaaaa cgacacgttc caacccttct tctttccaga attccaatgt
     2881 ctcaaaatta gttgctgaag cttgagtgga taaatggatc ggcagacctg gggcttctgt
     2941 cgcacaaatt tcgatcaaag ctggatcaga aacaatcact gcagaaatac cgacatcacg
     3001 caaactgcgg aaaaattccc ctgctccatc ttcatttcct tcatgggtga ccatattagc
     3061 agcaacgtat accttcgcat catgttcttt tgcaaaagca actccttcag ccatttcttc
     3121 ataagtaaaa ttacctgcac ggcttcttaa accatatgca ttcccgccaa tatatactgc
     3181 atctgctcca taatggatcg cggtttttaa cttttccagt gttcctgctg gcgctaggat
     3241 ttctggtcgt ttcaattttc tttttactgt catttctttc atcatactcc ttatttgata
     3301 gcagaaggat caatataaaa gaatcctgta tctaattcac ggttttcagg atgcaattta
     3361 ctgatttcgc tagtcatttt ttccgctttt tcttgatccc agctaccttc taaaatttgt
     3421 ttttttgctt cggcgaatag tcgagtgatg tttacgaatt tttcgcctgg agtaaataat
     3481 ccgtccaatt tccatgtaca gtaatgatgc tgataaagtt cattcaattc attcgagaga
     3541 ttcagatcat tgctggcaaa aatatgtgtg ccatgactat cttcataaat agaataatgt
     3601 gtctcttctt ttttaggttc cgagataaaa agacctcttt cacgatcttt ttgttcgtct
     3661 tgtttcgtat agttatagta attctgtagc agtggacgtt tggattgatg gatgcatgta
     3721 gcaccataca caagggtttc tacaggaata tcgagttttt cttccatcgc cttcatctct
     3781 tcaaaaggaa cttcacgagc aagtactgct ccaactgcgc cttttttcgc ccagaaattg
     3841 atttgacgtg cgctcgttac taatgtttca gcatcataaa tatacggtat acgcaattca
     3901 ggattttttt tcatcgtata aataatacct gtatcaccaa ctgtgatcac atctacgttt
     3961 atttctttta aaaaagccaa atattcaggt acatttttca ttttttcagg atgaatgatc
     4021 ccatttacag caatattcgc ttttttacca tacttgtgtg ctaattctac caattgtcgc
     4081 tgttcttctc gtgtaaatga agcaggcaga cgcaatccaa attgctcttc accgaaatat
     4141 agtgtatctg cacctgcagc aagcaactgt tctgcttgtt caatagtttc tactgtggtt
     4201 atgagttcga tcataatttt ctcctcctac aacttcataa tagtagcata gctctaaaaa
     4261 ctttccaata cttcttctat ttaaagtcgg atttttgaaa attatttcac aaaaaatcaa
     4321 aataaagttc gatatattct gtccaaatat aaaaagagat tgtgacaaaa atcttgtcac
     4381 aacctcttaa tcaagtctta ctattataaa aattcagcta cacaggccaa gtaactatag
     4441 ggaacctact gtttcctttt taggtccgcg aacattcaat gtgatcttac cttttgaagc
     4501 acctactgtt acgtggtcac ctaaacgaat ttctccgcca agcaaagcct cgctcaatcg
     4561 atcttcaatt tctttttgaa gtgcgcgacg gattggacgt gcaccatatt caggatcaaa
     4621 tccagctttc ccgatcacat cgattgcaga tggtgtgatt ttcacttgaa tatcttgttc
     4681 tttcaatcgt ttgataatcg ctttgctcat gatttggacg attgtatgga tttcatcttt
     4741 actcaatgaa tggaagacga cagtctcatc gattcgattc aagaattcag gtcggaaagc
     4801 tttctttaac tcttctaaga tacgtttttg catggctttg tgatctttag taacatcttg
     4861 gacattgaat ccgacatttt tttcttctcg aatttgtgtc gctccgatat ttgaagtcat
     4921 aatcatgatt gtatttctaa agtccacttt tctaccttta gaatctgtta aatgaccatc
     4981 atccaatact tgcagaagta aattgaaaac gtctggatgt gctttctcca cttcatctaa
     5041 gagaatcact gaatatggtt tttgacgaat tttttctgtc aactgtccac cttcatcata
     5101 ccctacgtat cctggaggag acccgattaa tcggcttgta ctgtattttt ccataaattc
     5161 tgacatatca acacggatca gggcatcttc acttccaaac atgacttctg aaagtgcttt
     5221 ggccaattct gtttttccga caccagttgg tccaaggaac atgaatgaac caattggacg
     5281 atcaggatct ttcaatccgc ttcgtgcacg gcggatcgca cgtgaaactg ctttgacagc
     5341 ctcatcttga ccaacgacac gttcatgcag aagtccttcc agttctaaca agcgttcgct
     5401 ttcttttttc tccaactgct gtagagggac accagtccac tccgatacaa ctgtagcaac
     5461 atcttcttct gttacacgat cagaataacc ttgctcctgt tttgcttcaa ttgcagaaac
     5521 tttttgcagc tttttacgta gtgctttttc ttgtctgcgt aattgagcag cattttcaaa
     5581 gtcttgtttt tggatcgctg cttccttttc ttgtactagt tcatcgattt ctagtttgat
     5641 cacagttgat tttgttaagt gatcggtttg atccaaacgg actttcgctg ctgattcatc
     5701 gatcaaatca attgctttat cgggtagttg acgagaagtg atataacgaa cagaaagatt
     5761 gactgctgct cgtacagctt catctgtgat ctccactcca tggtgctctt catatcttgt
     5821 acgtaatcct tgaagaatca cttctgcttc ttcaggagtt ggctcatcta cttgaatgcg
     5881 agcaaaacgt cgctctaatg cagaatcttt ttcgatatat ttttgatatt catctagggt
     5941 cgttgctccg atcgtttgta attctcctcg agctaacgcc ggtttcaaga tattcgaagc
     6001 atcaattgct ccttctgctc cacctgcacc aattaaggta tgcaattcat caatgaataa
     6061 gattacttga ccatcattat agatttcgtc gatgaccttt ttcattcgat cttcaaattc
     6121 accgcgatat ttcgtaccag caaccaaaga tcccatatca agcatcatca ggcgcttttc
     6181 ttgcatgtct tctggcactt cacctaaaat gattttttga gctaaccctt cagcaatggc
     6241 tgttttgcct acaccaggtt cacccaccag tacagggtta ttctttgtcc gtcggctaag
     6301 aatctggata agccgtttca cttctctcga acgaccaaca actgggtcca gcttgttttc
     6361 ttttgctaat ttcgtcaaat cacgtgctaa tgaatctagc gtaggtgttc cttgcggtgt
     6421 ttgccgttta tcttgtacat ttacatttct acgacgattc attccttttc cagcagaatc
     6481 accagctagc cccatttttt gtttcagaac ttgacgggct tttgaaaggc tgatacctaa
     6541 gttgatcatg attcgagtag ccatgatact ttcatctttt aataagctca tcaaaatatg
     6601 ttctgttcca actttttgag aaccagtttt cttcacttcg ctgctagcta aagcaaaaac
     6661 ttgtttcatt cttggtgaat atggaagaaa gactccttta gggtaagaac ggataccacc
     6721 ataaccaatc agatgttcaa tttcttcata aatttccgtt tcattcaaat ccatttcacg
     6781 taatactttt ccagcaattc cattttgctc gaccactaag gctagtaaaa catgttctga
     6841 actaactgat tgatgtttca accgttttgc ttcttcttgt gcaatttcta atactgcttg
     6901 ggctctttca gtaaaaagtt cttccatcta atctcacttc ccatcttcat aacttaaacg
     6961 ttccagaaat gctcggagaa tccgagcacg tgtatgatct tcatattcat tgaaattcaa
     7021 tgtgtttttt gcaattgcac tcaacatcaa gtttccttct ttttttgtaa ttatttggtc
     7081 ttcataaagt ttttgaataa tagcaaaagc atttttctca ctgattgtat catcaaataa
     7141 ctgatttatc tgctcaagta attgtttttt atctgaaatc cttacttttg caattcgtat
     7201 ataaccacca ccaccacgtt tgctttctac tagataacca cgttggatgg taaaacgggt
     7261 attaatcaca tagttgattt gtgaaggaac acagttaaag aggtctgcca tttcaatcct
     7321 tcgaatctcg atcatttcac tattttccag aattttcttt atatatgatt caattaaatc
     7381 cgaggtattc tgatgtgcca tcttattcat tccctttgac taatattgac cttaattata
     7441 acgaatttga taatagaaag aaagaagaac gctttttgtt aaataaatcc tttagaatca
     7501 acaaaaaaaa atacggaata tactattccg tatcgacgcg cccaagagga gtcgaacccc
     7561 taaccttttg atccgtagtc aaacactcta tccagttgag ctatgggcgc atattcaatt
     7621 taaaaaaatt ataaatcatt tgaaaacaaa aagcaagata aaaaaaatcg ggaagacagg
     7681 attcgaacct gcgacccctt ggtcccaaac caagtgctct accaagctga gctacttccc
     7741 gtttaaaaaa taaaaaagac
//
LOCUS       DAYYGO010000119         7616 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000119 DAYYGO010000000
VERSION     DAYYGO010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.122"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(45..743)
                     /locus_tag="ACHA5T_002387"
                     /pseudo
     CDS             complement(45..743)
                     /locus_tag="ACHA5T_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            822..998
                     /locus_tag="ACHA5T_002388"
                     /pseudo
     CDS             822..998
                     /locus_tag="ACHA5T_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1174..1743)
                     /locus_tag="ACHA5T_002389"
     CDS             complement(1174..1743)
                     /locus_tag="ACHA5T_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM8887931.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1817..2140)
                     /locus_tag="ACHA5T_002390"
     CDS             complement(1817..2140)
                     /locus_tag="ACHA5T_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887932.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2294..3106)
                     /locus_tag="ACHA5T_002391"
     CDS             complement(2294..3106)
                     /locus_tag="ACHA5T_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887933.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3433..4308
                     /locus_tag="ACHA5T_002392"
     CDS             3433..4308
                     /locus_tag="ACHA5T_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8887934.1"
                     /translation="MGKRSTIQLMIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGT
                     FSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLE
                     AMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDD
                     ELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAI
                     QLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKK
                     QIIHTLVAQIPIPVR"
     gene            4322..4645
                     /locus_tag="ACHA5T_002393"
     CDS             4322..4645
                     /locus_tag="ACHA5T_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887935.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            4642..5394
                     /locus_tag="ACHA5T_002394"
     CDS             4642..5394
                     /locus_tag="ACHA5T_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFM8887936.1"
                     /translation="MILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKR
                     EISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKL
                     SAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPELLILG
                     NRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKD
                     RTVCLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5384..7555
                     /locus_tag="ACHA5T_002395"
                     /pseudo
     CDS             5384..7555
                     /locus_tag="ACHA5T_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         2265 a         1507 c         1375 g         2469 t
ORIGIN      
        1 aatagttcaa cttttggggt tcagttcaaa gaggggggga ttttttatcg ttgtggaata
       61 tttaaatacc aacgcttgtc atgaaaatct tgtgctccac ccttagtatt tccttctgga
      121 tcgtttgtcg cacgcatcat gacgtatact ttcttattag ggaaatcacg catattgaaa
      181 gatacatgat agccaacatt tcctaaagta ttataagctt gatttacgtc tggtctataa
      241 atcccatcag ctcttactct agctagttct tttccagtat tgtagtccat aatgaaaata
      301 tactcgtatt tataattagc aatgtgccat ccagctacat gcaagtttgc gttttcgatt
      361 tctccgaact gatcaatgtg ggcgtaattt gttccatctg tcagcgtaga atttgcagca
      421 cctgctcgag ttggatcaat tactggttta tcctccgaag tagttggatt atcatccgta
      481 aaaccatgag ccaaatcata ggctagtttt tctttactta cacccatttc agaaagataa
      541 ccgtaaggat ctgtatgatc gccccaaata ttttgtgtta cccataaatg cgatttgatt
      601 cctggttggt tataaggagt gtctaatgtt aatggaatac catatttcat tgctgaatct
      661 cttgccaatt caacgtatac tttatagttc ttttcaaaag tttctttatc atgtgtatgt
      721 tgtaactcga tttgcacagg acttttggct cacgaggttg tcctactcta catcttgctg
      781 atagatatgc tgaaaggaaa acaccaacat tatgttttgc tttttcagct agaacgagac
      841 aagcaggaat acctttagaa gatatcaaag gttttttagg tcataaagat gtttccacta
      901 cacaagtcta tgcccatatt tctcccgaag tgaaaaaaag atcaatgaat caactagaaa
      961 actatataga agagcaaata aaaaagcact caaattgagt gcttttttaa gctcactatc
     1021 actaaaacta tcactaattt tatttataag cgaacatgtt tctatcactg attttttaaa
     1081 tctagcgaaa aacccttatc ttaaaggcgt tcgttcaatt ctttggcaag gtcttcaaag
     1141 cctggcttgc ctagaagtgc gaacatggtt ttcttatagc tcatgtagaa aaattttctt
     1201 gatgatgatt ttatccatca attgagattg actgttttgc tctcctatga tttcagctat
     1261 gattgtccga ttttgcggtt ttcttcttaa ctggtagttg attttcccag tttccatcaa
     1321 ctcgctaagc atatccacga ctccgccaaa tccggtcttt ttcgtaataa aacctagtgc
     1381 taccattcgt tcatctagat tttttgctgt aatcgtcaca ccagtaatga gcgagtgctg
     1441 taatgtttgg gccagctctt ccatcccttc agcattggat acgacggaag ctgttttgtt
     1501 aataggttct tcgtttattt cttttggttc tttcctctcc actttcactg tttgacgaag
     1561 gacatctagt tcttgcaaga gcgtttcgta ttgttcttgg gtcagcatca cgccagctac
     1621 tttttcacga ttaaatatat atacgccagt tcccgcgtct cgcgcttgag caaatacatc
     1681 catcggcgat cgtttcactt ctgtgatcga agatgtggga acttctaatt tctttaattc
     1741 caatggattt cctcctttac taatcgcttt ttattatagc acagactgac ttgttttgat
     1801 cttttttcca aaggttttat cggtttttgc ctaaccagta attgatatcg gtaagaagtc
     1861 tttcgatacc aaatgaagga tggtacagat acgttttttg atcttcgtta tggtctagtg
     1921 gaaaaaaatg atcgatgatc actagatctg cataccgagg cggcttattt cgagtacata
     1981 catgtataga gatccgtttt cgatgtctta gcgttttgtt cagtacccca gcaagtcttc
     2041 ttgcttcttg gtgttcatag cgaaaaaaaa agacgatttt cacttcagga gggtggacat
     2101 ctaaataccc tcgttcctgg agtgttttca gtaaaagcat cccccactct actgcggggt
     2161 gtgcaagtgc ttgttggatc gctagccgaa attgatatag tttatctttg gaattgggaa
     2221 atttttctaa tgtttcctga taataacgaa cttcttctat cagccggtca tggataccaa
     2281 ttacgtaacg aagtcattct tggtaaagta cttttgtcag ctttgatttg ctttcgctct
     2341 caaattcgct attcagccaa aacagcgaat caagcttttc agtcacaatc gtcaatttcc
     2401 gatagtcttg tgtctcttct atccgtttcc accgcttttc agtaatctgg ttacagctca
     2461 agcgcaaaat tgctagataa tttttttctt ttattggcaa aatctgctcc tctctaaata
     2521 gcaattcagg aaacaattca aagaatagtg ttcgactgtc tcctaaaatc tcgatttctg
     2581 ccggatcctt tctttgcttc aataattgga tgaacatctc agaaaaggac tgctctccat
     2641 catctgcaaa aaaatgcgga aaatctttca aaaagcaaaa cagcagatat cttacttttc
     2701 tttctgaacc aaagatctgt gcttttttat aagaaaaaga aaggtggtac tcgccaaacc
     2761 actttctcaa tcgctcttgc gtagaacgat cgtacttctg ctcgtaaggt tcattcaata
     2821 gcagattttt cagtaaacaa aacgggggtg attggatcag aaaaacaaac aagagataag
     2881 gttcgctata tccttgatca taaactaact gaacggtttt cttatgaaga atcagctgaa
     2941 tgggacagtc gctttccgcc aaacgtttca gatgccgatc cactgtttta acatcgattt
     3001 tcagttcttc agctaaatca gcttttcgcc agatcaaatc tttcattaga ttgattagtg
     3061 aaaataaatt ctgtgcattt tttgataata gtttttgcca tctcatcgat catttctcac
     3121 ctgatttcta aacatattgt ttgatcaaaa tgggttcact cctaaaaaga taaaaaactc
     3181 cctttctatt tatttaaata cgcaaatctt cctcattatt ttttatttta ctcaatttaa
     3241 tcgtattctc atttcaaaac gaatgtactt taaaaaaaca taataagatc actttgcttg
     3301 taggatgtat tcccttccat tacaatgaaa taaatagcga ttttatgaga aggtgatgtg
     3361 aaaatggaag aaaaaataga agaatccaaa caaaaaatcg atcagttgat ataagaatta
     3421 gaaaaagcgg tcatcggcaa acgttcaact atccaattga tgatcgtggc cctattagca
     3481 ggtggtcatg tcttattcga agatattccc ggtgtaggta aaacgttact ggtcaaagca
     3541 ctagcaaaag ctgtacaggg aaccttcagc agagtccaat gtactcctga tctgctgcct
     3601 agtgatattc ttggtttttc cgtatacaat tcacaatcaa aagagtttga atttcgtccg
     3661 ggaccgatct ttaccacgat cttgttagca gatgagatca atcgtacgac tcctcgtact
     3721 cagtcagcac ttttagaagc aatggccgag aatcatgcaa cgatcgacaa caatacatac
     3781 cctctagaca atcatttttt tgtcttagct acgcaaaatc caatcgagta cgaaggaaca
     3841 tatcctttgc cggaagcaca gctcgatcgt tttttattcc gactacaaat cggttatcct
     3901 tcttttgatg acgaactttt gctacttttg gataaattcg aaaaagtgtt ggataactta
     3961 aatgaagtat tgaacagtta tgaaatagaa gaaataaaaa caaatgtggc gaatgtcttt
     4021 gtcagtcccg aagtggcatc ttatgccttg cagttagtgt cagcaacaag aagtcatgct
     4081 gccatccagc ttggcatcag tccacgagga agcctttcct ttattcaggc agcaaaagct
     4141 tatgctttaa tccatgggag aaattacgtc acgcccaaag atttgcagga tttagttcct
     4201 tacgtgtttt cccatcgcct gattttttac gatcgttcat tgaaggaaga cgaaaagaaa
     4261 caaatcattc atactcttgt tgcacaaatt cctattcctg tgaggtagat tgaggtagac
     4321 aatggaaaaa atcaaagatt atttcattaa ttttgtgtta tttctggtat atttcatcat
     4381 tgtgcttttt gctcttgtat tcaacaattc tttaggctgg ttcttgttgg ccttttttac
     4441 tctttatgta tcatggagct tgatttcatt aagcagcttt ctaaagaaac tttcggttac
     4501 ttgtacattg cagccactcg tttatcgcaa aaaaagtatg caggttattt tttccatagc
     4561 aaaaaagaga actttttctt tacctctccc tcgtttacaa atcggcttcc cagcaatctt
     4621 tgcagaaaaa aacaagagat tttgattctt acaaataaac cgcaagaagt ccaattgctt
     4681 tggcagccaa aagaaagagg atgccatgaa acattaactg tcatttttac agcctatgat
     4741 agtctaggac ttttcagaaa aagtgcaaaa agagagatct cttttcctgt gactgttctg
     4801 cctgcttttt gtaagattca agcagaaaaa ttacagagag ttctggaaaa aaaacagcag
     4861 ctgaacgcgt accaaagagg tcttgatttt cgggaacatc gcgcttatcg tccaggtgat
     4921 tctttcaacc gaatcgattg gaaattgtct gctcatactc aagagtggtt ataccgtgaa
     4981 tacgattatc aagaagaaga atgttctcct cttttttgct tttggggttc gcccagtcca
     5041 aaatttgaac ctctgctaga cttgtatttt tctttatggc atttgagaaa agaaaagcag
     5101 ccggaacttc tgatattagg caaccgcgcc tttcatggca aagaccccgg tcatacctat
     5161 tttgcttctt tggaagctgg cgatgagaaa gcttttttcg cacacttgaa aaaatatgca
     5221 aaaaaacaaa tcgtcctctt tgttcctgag aattctcctg aagtctcaga agctattgaa
     5281 acattgtcaa aagatcgaac ggtttgtctt gtttatttcg tagaaaaaca actcatggtc
     5341 caagaaaaag acaagatatg ttcattgcca ggaggtgaat ggattgacga ttaaacggga
     5401 aaaatggctg cgtggcgttt actcctttgt gatactaagt gtaggtgctc cgatatttgt
     5461 agtagctaat caattgcaaa acagctacct tcttatccta actattggtg tcctatgcct
     5521 tattgtctgc tgtattccta aaaaatgggt acgtttgctg ctttctttgc cagtaatgat
     5581 aggttgtttg tttttatatt ttccttcgaa gacattttcc atattatgga tcttacagtt
     5641 tttttcgtca gtcacagagg aattcttcca tctatcccaa ggtaaacttt attatttgcc
     5701 agaaaagaca gctttttttc tcataatgtt gctgatctat ttatttatta cattgaccgt
     5761 ggattacgac tattggtacg caccctttct ttctttgatg gcttacttca tgatgcttac
     5821 cgtttttcgg aaaaaagatt tactgtacga aatgattgcg gtagtaactg ttttgttcgt
     5881 gtatctggca gcaaggcaat ttttctccat ccggttgttg tcgggaaaca ctcgattcca
     5941 gtcattgact acagttctgc ttatttgttt cttaactttt agcgctatct tgcctttata
     6001 tcttccgaaa gtacaccaat ctctcgagga aacgagtgaa ccgatccgag aatatcttaa
     6061 tgatgaaggg ctttatgaca cactccatga gtatcaatta accggaagtg caagaacagg
     6121 cttcagtgag aatgatgaac agcttggtgg acctttatat gataatggcg aagttgtttt
     6181 tacagcgaac caaaaaggaa atggctattg gaagatcgaa aataaaaatc attacaccgg
     6241 ttctggatgg atagaagatc aggacgcacc catccaacca atccaagaat tccctgttga
     6301 catcacgagc taccaaaccg ttatagaaga caaagaaaac attacattga ccctaaaaaa
     6361 acaagttttc ttaccttatc cttatggaaa tactaccttg actgattttt cattagctag
     6421 tggacaaacg ctttactatt tgcctgaaag ccagcgattc attcctgtcc cgaatgaatc
     6481 gacacgttct gcaactttac aaacagaaag gttagttttt acgagagaag aattaaaaag
     6541 cagtcagtta aaagctgatg atttagaaaa tcagcagaca aatcttcaat tgccaaaaag
     6601 tttgcctaaa cgtattgagg acttggcggt cgaattgacc aaaaatgaag caactttaat
     6661 tcgtaaagtc gaagctgtct aacagtattt aaaaacaagc ggaaatttcc gctattccaa
     6721 aacggatacc gggcatactc caaaagaaca cgattatgtg gattatttcc tctttgattc
     6781 aaaagtcggc tattgtgaca acttttcaac ttctatggtc gtccttcttc gaacacttgg
     6841 tatcccagct cgctggacaa aaggttttac aaatgggctc agaacaggca cagataatca
     6901 agggaatgag gaatacagca ttctcaatag tcatgctcat tcttggccag aggtatactt
     6961 tcctggattt ggctggcttc catttgaacc aaccccttca tttgccaatc caatgggttc
     7021 acaaccagtt gctgaaacaa ccgaaaccgt ccagcctgct atcgaacaac caaatgaaag
     7081 cgaaaatatg gcagaaacta tttccagtga gagatctgag gtcattccat cagaagaaac
     7141 aacactcaca ccagcttata aaatcgtctt attcagcctt ggagtgtgtt tatccgtctt
     7201 gttgttcttt ctattgttat tcagaaaatc tctgcgctgg acaagttatc ttatctatct
     7261 tcattttttt gcttctacta atacgattgc atatcaaaaa cttcttaacc ctttggaaac
     7321 attggtaccg cgcaaacccg cagaatcttt gcaggaatat agcctgcgaa tagaacaaga
     7381 attcaagcta gatctgcgtt tcattcgatt aactactcaa tatgaagcag aaatatatgg
     7441 acgacaagca gatccggtaa aattcgataa aaaaacctat ttagaaataa cgcatataat
     7501 aagaaaaatg gtatacaaaa aaattctgac aaaagggagc cgcttcaaaa aataagataa
     7561 aatttctgga gaatctactt ctggaatgcc gagaaccagc tgattgagta tcgatt
//
LOCUS       DAYYGO010000120         7366 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000120 DAYYGO010000000
VERSION     DAYYGO010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.123"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(13..765)
                     /locus_tag="ACHA5T_002396"
     CDS             complement(13..765)
                     /locus_tag="ACHA5T_002396"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8887937.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(1129..3795)
                     /locus_tag="ACHA5T_002397"
     CDS             complement(1129..3795)
                     /locus_tag="ACHA5T_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8887938.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            4136..5587
                     /gene="cls"
                     /locus_tag="ACHA5T_002398"
     CDS             4136..5587
                     /gene="cls"
                     /locus_tag="ACHA5T_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFM8887939.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            complement(5916..6731)
                     /gene="thiD"
                     /locus_tag="ACHA5T_002399"
     CDS             complement(5916..6731)
                     /gene="thiD"
                     /locus_tag="ACHA5T_002399"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFM8887940.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            7025..7300
                     /locus_tag="ACHA5T_002400"
     CDS             7025..7300
                     /locus_tag="ACHA5T_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887941.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
BASE COUNT         2168 a         1533 c         1252 g         2413 t
ORIGIN      
        1 aaaaagaacc cttcacgagt cgtgaaaggt tcttttttta tctgcggacc aaaggagcag
       61 tctataggtc agcttagtcg tactaaggat ttctcgtttt tcactgaata gtttgcgaat
      121 gtacctggtc acaacaggat cttttgcgta cgcgtcatag tatagcttca tcaattcttc
      181 agtaattgat tcctgtgtaa aataagtaaa atcttttttc ttcactttga atccttcatc
      241 aattaagtat gtttcaaatt gatccattac aactggcgat gtattaggat cttcttctat
      301 cattcctagt tgcttttcga taggataaaa aacatcatcg attgattcca ctactctgat
      361 tatcacgcac catttgcttg actttttcat caatttttct aattgaataa gatgtaccaa
      421 tgctggattt gctgctgtaa aaaccatttc atatgtctca tttgattgga taaattcttc
      481 taacgactgt ttgataaagg aaacatttgg caactcattt ttttctgctt ctttttttgc
      541 attttctagc attcctgaag aaaaatcgat caaatcatag cgattaattt cttttgctaa
      601 ggaaagaaat ctgccagatc ctccagcaac gtctgctgct gtttcagcag gcaggacttt
      661 ctccgttttc agataatttt ttacatcttc agcaatagtt acttgcgatt cttgctggat
      721 ttcataatat tcttcagcaa attcatccca ttctttttca ttcatttgat ttcctctaat
      781 tttttgttat atgttttcac ttttcggaat aaatcattct cctcttttat ctattctatc
      841 tcttttgatg aataataaaa tgattggctt tccgtctatc ataaataaaa aaagggactg
      901 tgaccaaagt cgtgtcaaag tccctctttc cgaataaaca gtgtccaatc agatgaccct
      961 cagtattagc tcaaaatgct gattttacaa cttcttcctt aaacggtgtt ctcctgccaa
     1021 ctcttcggtc acaagtcaca atattcaaca aacatgagaa gctgtttgtt gaaattgcac
     1081 cttgtgactc agaattaaac ggcaggctca caacctcgtt gattcgtttt atttatttcc
     1141 ttgtactcgt acaatgatca atttagtgat ttccatcaaa acaattgtca caagtgcgat
     1201 tcccatacat gtaaagactt ctgtcaatcc aaatgctgat gggatagaga agacttcttt
     1261 cagtcctggg atcattgtta agcagtaaag cgcgctacat acaactacag ctagtaagac
     1321 acttctgtta gagaagaacc ctgcttggat agctgtttgt gtgtttgaac gtgctgggaa
     1381 tgtttgcaat gtccgacacc aaatcaatgt tgaaaatgac atggctacac cgaattcagg
     1441 tgaatgctgc atgccaagcc attgagcaaa gataacagca atcccaatca gcacaccacg
     1501 atacgttaca gaaatcaatg ttttgtccgt aaagattcct gaatttggat ctcttggcgg
     1561 gcggttcatc gtagcaggtt cagctttttc tgtacctaat gcaattgctg gcagtgaatc
     1621 attgactaaa ttgatgaaca acagttgtaa tgctgtaaat ggattggacc agtcagcaac
     1681 taaggcaaag atgatcgcaa tgatcgcacc taaatttcct gcaaatagat aagaaatagc
     1741 tttcttgata ttatcaaaca cattacgtcc tacttctact gcgctgacaa tagaagcaaa
     1801 gttatcatcc gtcaatacca tcgctgctgc atcttttgcc acatctgtac ccgttcccat
     1861 agcaattccg atgtctgctt gtttcaatgc tggtgcgtca tttacaccat cacctgtcat
     1921 agctgagata tgacctttat tttgccaagc acgaacgata cgaattttat tctcaggtga
     1981 aacacgtgca taaacagtga ctttttctaa aacttcgttt agttgatctt ctgtcaattc
     2041 atctagttcc gtcccggtca aagccaggtt tccttcttca aaaatcccga tttgttttgc
     2101 gatagcaaca gctgttgttt tgtggtcacc agtaatcatg attgttttga ttcctgcact
     2161 tcttgcttct gttaccgcct gagtcacttc agcacgtgga ggatcgatca tagcaagcaa
     2221 accaacaagg atcaaatcgt cttcatcttc aaatgataaa tcaaaattat caagtggacg
     2281 gtaagcaaaa gctagtacac gtaatgcgcg tgttgaaaaa gcttcatttt gctcttggaa
     2341 ttttttcatc aattcatccg tcatcggaac gacttcacca tttagcaata cttttttact
     2401 gcgttggaag acgatatctg gtccgccttt aacaaacaag taatttttgt tatcaattgt
     2461 gtgtccagta gacattaatt ttcgatctga gtcaaaagga agttctgctt gtcttgggta
     2521 gttgtttcgg atttcctgat aaggctggtt taatttttca ctaaagtcga taaacgctac
     2581 ttccgtaggg tcgcccaatt tagtaccgtc ttcactgatc gatgcatcat tcgctaaaac
     2641 actgatttcg atcaagcgac gttcgtcagc tgtccatttt gaagggtcat ttgtgaaatc
     2701 cccagtatcg ccattaccta aaaagtagtc aacaactgtc attttgtttt gtgtcaatgt
     2761 accagtctta tctgtacaga tgatacttgt cgaaccaagt gtttcaactg ctggaagctt
     2821 ccgaataatg gcatgacgtt ttgccatttt attcgttccc aaagataaca cgattgttac
     2881 gatagactgt aatgcttctg gaatagcagc tacggctacg gctacggcaa acatcactgc
     2941 gctgacgata tctgcagtca tatctcctgt accttctcca agataaatac gaaacagttg
     3001 gatacctaaa atcaataaag acaatgccaa aatagccaaa cttagttttt tactgaattt
     3061 atccagacct cgttgtaacg gagtgacttg ctcagttgtt gattccaaaa ggccagcaac
     3121 ttgtccgatc tccgtattat tcccagttga agtcactaag aatttaccgc gtccgtaagt
     3181 tacgatcgtt ccactgaata ccatatttgt acgatctccg attggtaccg tacttgtgat
     3241 atccgttact tctttttctg cagaagtcga ctctcctgtc agcatacctt catctacttt
     3301 taatgatccc gcatctagta aacgtccatc agcaggtaca taatcgcctg cgtctaacaa
     3361 cacgatatct cctaccacaa gttcgtttgc cggaatcgtt ttttccttac catcacgaat
     3421 cactcgagca tcgggcgctg aaaggttttt caacgcttct aaagaacctt ctgctttttt
     3481 tgtttggaca acactgatta tagaattcaa catcaatact gcaaaaataa ctaagaattc
     3541 gatgtagtca cccatgacca tctgaatcag tgcgacaatc agcaatacga taaccattgc
     3601 atctttgaaa gtttctaaga acaatttcca agttggcttc ttcggctttt cttttagctg
     3661 gttcaagcca tctctttcta atttttcctt cgcttgcgtg gtgctcaatc catcggttga
     3721 gctctgcgtt tgctgcatca acgagtcgat cgtttcttta tagaccgctt gttgtgcttg
     3781 ttccttgttt tccaaatttc atcccccatt ttttgatctt ggccctagtc ctgtctgaaa
     3841 aaaaagagac ctaagacaaa tacgcatttg tcttaggtct caccatttca tacaatacca
     3901 gaaaaccatt tggttaactt actgttgata ttatcgcaac aaacgttgcc ggttactccc
     3961 ctaaggagat attcaactgt actaatatcc taccttatta acctacaaaa agcaactact
     4021 ttagcccgat ttacctacat tttcatgaaa ggagtgaaaa gtatattcac cttggtaggc
     4081 ttctttcttc tatcatgcta tactaatgtt attgtatgta aggaggaaca tttttgtggt
     4141 atctagtatt ataaccgccc tttatctatt aaatgcactt attgctttgg tggctatttt
     4201 gatcaaaccc cgagatgtag cagccatttg ggcatggctc ttagtattgt ttgcccttcc
     4261 tggggtgggc tttgtattat atttattttt cggacgtgga ttaacggata agaaaaaatt
     4321 ctatctccga caaagtgact tgaaagaatt agaaaacttt cagtctttta gaggagatac
     4381 cattgaacat tacgatcctg acatgggcga taaagacaaa cagcaatttg ttgacttctt
     4441 ctcttcatta aatcgtatgc cgctgacaag aatgaattct gtcactcttc tcacagacgg
     4501 acaagagaaa ttggattcac tgcttcaaga tctaaaaaaa gccaaacatt cgatccatat
     4561 cgaatattac gcatttgtga cagataatat cggccagcaa gtcttacatg ttttagaaga
     4621 aaaagccgca gaaggcgtgg aagttcgaat attatatgat gcatttggct ctcatggcac
     4681 aaaagcaaaa gatttcaatc gtctaatcaa aaatggtgga catgtccata catttgttac
     4741 ctcacaaagg gcattacttc gtttccgatt gaattaccat gatcaccgaa aaatcgttgt
     4801 gatcgatgga aagattagtt ataccggtgg tttcaatatt gccaatcaat atgtaaatac
     4861 aacaaaaaaa ttcggctatt ggcgcgatac gcatatacgg attttcggtg ccgcttcttc
     4921 tttgctccag cttcgcttct taacagactg gaacgtctcg gtacctgaag aaaaaaaggt
     4981 cggctatcat ttgaattatt tctttaaaaa agcagatcga gatgaatcta agcttgctga
     5041 tacatccatc cagcttgttt caagcggacc gaataacgaa agggaacaaa tcaagctttc
     5101 atttatcaaa ttgattactt ctgctaaaaa acgtgtttgg atacagacac cttaccttgt
     5161 tcctgatgaa agtgtcattg ctgctttaaa aatcgcaact gcctctggtg tagatgtgaa
     5221 aatcatgatt cccaacaaac cggatcatcc ttttatttat cgagcaacac aatattatgc
     5281 tcggcagctg atcaaggaaa atgtacaaat ccttgtctat gagaacggct tcctccatgc
     5341 aaaaacattg ataatggatg atgaaatctg catggtaggt tcagcaaatc aagatattcg
     5401 tagctaccga ttgaattttg aaacaagtgc tgtcatttac gatcctgagt ttttagaaga
     5461 acttgctact cagttcaaag aagatgagac acattgttca tccatgacaa ctgaaacagt
     5521 caaggaaatg tctaactggc tattattcaa gcaacaaatt tctcgattat tttctccaat
     5581 cctataattt ttttattcaa gtagagaaaa atatactaag attaaaggga ctgtgacaaa
     5641 aatcttgtca cagtcccttc ttccgaacaa acggtgttct cctgccaact cctcggtcac
     5701 aagtcacaat attcaacaaa catgagaagc tgttttttga acttgttcct tgtgactcgg
     5761 agctggacgg caggctcacg acctctttct taaacggtgt tcaaaagctt gcatctgcgg
     5821 taataagtcc aaccaagtta aaaacataag aagctgtttt tacttggctg ttcttattac
     5881 ttgatgcagg gcaacttttt cacaacttct ttcttttact ctaattcacc agctgcccaa
     5941 tgattgatgg ggccatattt gtgccctaca tttatttctt cacgaattgc acggtttaca
     6001 tagtcttttg ccaatcggat cgcttgttca actgtttttc ctttcgctaa ctcagctgca
     6061 atacatgcag atagcgaatc gccagttccg ttgatccggt cggtaggata aaatggtgca
     6121 tttaaccaaa atgattttcc tgattcgaac aatacgtagt cattgactgt ttcttgctgc
     6181 gcatttttag catgatgtcc tttgatcata acgttttttg cacccatatc ttgcagttct
     6241 tttgctgctt gcacaaaatc ttcttcagct gctaatgtcc gtccagtcag cttttctgct
     6301 tcataaaaat taggtgtcag tacttcggcc aaagggacaa gcttttcttt caagctttgc
     6361 aaagcagatt cttctagtaa aagatttcca tgtttcgtta cgattacggg atccacgact
     6421 aatggtccaa aatcatattt ttggtaattt tttacaactg tctcaatcaa ctttgaatct
     6481 gctagcatac ctgttttagc tgcacgtatt tgaaagtcct tcgcaatcag ttcaaattct
     6541 ttgtcgataa aatctgttgg caatgtcaca cttgctccga ttccgtaaga attacctgca
     6601 acacatgcgg tcatgacaga aacaccgtaa acatttcttg taaagaatgt atgcatatct
     6661 gcttgcatcc ctgcgcttcc atcgctatct gatcctgcga tcgtcattgc ttgtacatac
     6721 tgatcttcca tcaaatcgat tcctccattt ttataaaaag atttttcata atactgttac
     6781 tgtttctatc atatgttttt caaagtatcc ctcagtaaat ataccactac cttttcttgg
     6841 taatcaaatt tatttgaaaa aacatttgca ataaatcaga aaatagtggt cgtttatttg
     6901 tattcgctat tctaataata aataaccact atttgattta tatatatttc agtatccttc
     6961 ttgttttcat ataaccaaag gcgctatgat ttccatatga aaaaaataaa gtcaggtgaa
     7021 aaaaatgaaa cttgaggata aaaaagtcat tttgaagaaa atcaaacgac taccagacca
     7081 aatagatggc tatacgatct gttctttcaa aaaagacagg agtataaaag cttatacgca
     7141 aaacacagaa aaaggaataa tctatttcct acatgaagaa ggttacgata cccaaacctt
     7201 tatgtttact gaaaaaaaag aatttcataa acagatgaaa aaactgattg aaaaagaatt
     7261 tccaagaagt cataaactct atgtgaatca gcaattttaa cttctaactg ttctttatta
     7321 attaaaaatg ttctgtaata aaataaaact ttataacaac tataac
//
LOCUS       DAYYGO010000121         7224 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000121 DAYYGO010000000
VERSION     DAYYGO010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.124"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            161..2836
                     /locus_tag="ACHA5T_002401"
     CDS             161..2836
                     /locus_tag="ACHA5T_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HFM8887942.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGIAKIEATFVEAQKKIWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            3018..3257
                     /locus_tag="ACHA5T_002402"
     CDS             3018..3257
                     /locus_tag="ACHA5T_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887943.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLAFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNGRVAKEWYLVHS"
     gene            3273..4646
                     /locus_tag="ACHA5T_002403"
     CDS             3273..4646
                     /locus_tag="ACHA5T_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8887944.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGGKADRPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLAYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEAHEAKVVRHIFQLYLTKKYGYKKLCQRLTQQKFFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKTIFLQAQEIRQSRCTAKKDTYPYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKHKVIQQKKQEAQWLKAFEEGNCSLEELKQQLQALQ
                     KPRSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDHVCRIEVTKEKTVCGIYFRQMTQ
                     NILEGNE"
     gene            4650..6287
                     /locus_tag="ACHA5T_002404"
     CDS             4650..6287
                     /locus_tag="ACHA5T_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8887945.1"
                     /translation="METTPTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMSYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPKPILVKGTHEPIISEEDY
                     QKVQERLVLEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQQGEPIQVKDVQRILTSLDRFLAQSEKKQVKALYRTFIEKITFDPNYKEILEITM
                     KFTPAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            6450..>7224
                     /locus_tag="ACHA5T_002405"
     CDS             6450..>7224
                     /locus_tag="ACHA5T_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887946.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYYTLCSKCNSFFGSEYVEEYIRFAEDNKNFLYNNTSLK
                     NGKSDLTHSIKKMNSLRVAKAIVAMFFSLNGDEDSMDKPFLDSVREYLSNPKSTLFPH
                     EDYKIIMNYYLPIGAPQNSKIKIPNDIVNDGDTHNVIKGFMPLDLSTGRKIRFSEIQY
                     EGIGFTLIDLKNSTFEIKEGLDLKNLLCADDKIKKIQLYK"
BASE COUNT         2539 a         1147 c         1434 g         2104 t
ORIGIN      
        1 taaaagaaaa aagttgtgat tttggaaatc acaacttttt tcttttgtca agattttgag
       61 tgggatatat ttttgtagaa ctgaagatca tgaaattatg agaggttgga ggtaatgtag
      121 tcatacaaat aaaaattttt tagaaagtga gtggtaatta atgaaaatga atcacacttc
      181 aattcagaag catttacaac tgttaaatac actggggatt tcttatgtta atttgagaac
      241 tttttctaca ttaaacaaga aaaccgtttc tcatattttt tctactgcag attctcaagc
      301 aattattaac tatttagaaa aacagaaatt gtctgaaggt acagtaaaca taacttataa
      361 tcctattaaa caagaggtga ttacacaaaa aaagcattct gtgagagaca atgatattga
      421 aaaaattcgg tttgtattta ttgatattga ccctaaaagg aaagagggaa gtgcaacaga
      481 tagcgaaaag aaaaaggcag aaaatgttat ggaacaagta gaacgttatt tgaaagaaaa
      541 gagaatagaa tcatttgtaa aagttgatag tgggaatggc taccatattt ttcttcccat
      601 aaatgaacaa ccaaataatc acgagacgat tcttactatt cagaattttc ttcaattact
      661 tcaccgaaga tttggagtag aagatgaagt tgatattgat acttctgttt ataatccttc
      721 aagattatgc aaattagttg gtacaccagc ggtcaaagga aagtccacaa aagagcgtcc
      781 gcatcgttta tctagtttcc tttctattcc cgataacata gatagaagta attgttttaa
      841 aattcttgaa cagatagttg aagaaaattc catgatgtca acctctagag taaaaagcca
      901 tccatctttt agagaagtgt taccttttat tcaagcagac tataaacaat ggttagctca
      961 ctatcctgaa ttaacttata ccataaaaga gggcgatcat aaaggagtta ccttacttat
     1021 atttgacgaa tgtccattaa gaaaacacac gaataattct aatggttctt gtttgtctgt
     1081 tacggctgat aataagattc gatttcattg tctgcatgct tcggataaaa ataaagatat
     1141 tcaagacttt gtaaaaaaat atccgttgcc aaaacctgta aatccttctg tagaatcatt
     1201 agaatccaat caagaatatt attttgacca gtttaaactg aatcaagaag gagtattttt
     1261 ggtgaaagaa ggtcaagaag aaaaagttgc ttgtccgatg tttattaagc aaatacgcaa
     1321 aaatatcgaa caaaaagtca ttacacttgt tcttgcctat tatgtcgatc attgttggga
     1381 tgaaatagaa atagaaggtg gatgtttaca aactaataat tttaaacaat tagcaaaaca
     1441 tggcttaatc attaaagcta gaagagaagc tgaagtcgtt gactttttac agattcaacg
     1501 ggagaatgct ccaatctttt ttgaacatga atctcttggc tggaaaaaag aaaatgaagc
     1561 aatgattttt cagttacaag attcttttgg acgtgaaatt ccttctgtat taactaaaaa
     1621 ctcatgctac caattaacaa ctaatggtac agaagcggaa tttgacgcat tggttcataa
     1681 gtatgtttta ggaacgaact tagaacttgc ttggtcgatt ggtattgctt ctattgtgtt
     1741 aggctattta aacgttacga aggctaatac ttttttgagt ttactaatta acctacaggg
     1801 caagagcaca acgggtaaaa ccactacgtt acactttatt gcctctttat ttggaaatcc
     1861 aactacggtt cttagaaata tgaatgctac caataaagcc attataaagc tagcatcaaa
     1921 caatcaggga ggtgttccac taatactaga tgagttagga gcagcaactc aaaataactt
     1981 tagctcgttg atctatcaat tagcttcagg agaagaaagg ctaagattag attctaattg
     2041 tcagcttatg ccacaagaga ctttttctgt tttgaccctt acctcttctg aagagcgttt
     2101 ggaaaattat ttcaatgaga aattaactgg tttgaaagtt cgatatatcg aattttctga
     2161 tatcaagttt acaaaggatg cgaatcacgc agagaaagtt aaaagtcttt cttctaatac
     2221 ttttggttgg ggtataaagt tatttctttt gaaactcttt gaacaaggga ttgcaaaaat
     2281 tgaagcaact tttgtcgaag ctcaaaaaaa aatatgggct gttttacctg aacattcttt
     2341 gaagcaacgc attggtaata atctagcttt gatttatagt ggagctctcc taattcaaga
     2401 tatactgggc tatcctttac gattagagca tatacagacg gtgcttatta attcgtacga
     2461 acagttaatt gtagatacta gtttaggaag tgagcaaacg gtatttgacc gagtgaaaac
     2521 gttgttggtg atgaatagcc ataaattttt aacaaacagt gacaacaaac atacgcccag
     2581 tacgatttgg ggaaaagtat caattcaaaa aaatggggat attaaggtaa atatttttcc
     2641 tatcccgttt gaggacttgt taaggcgaga atttcaagta aaggatctaa cctatctttt
     2701 tagacaattg cttgattcgg gtaatatgtg tgcagaaaaa gggagaagaa cgaaacgtat
     2761 tcacatgaat cgtcaagttc ttccaacata cgagattcta ttaccttctt cactcaaatc
     2821 gtattttcga ttgtaattta gaataaacca aagtatgaaa gtgaggcacc atgttatgga
     2881 aatatttctt attgatttag caaaacaact gattacttat ctgtatgatg acgagcaaac
     2941 ccaaacggaa gtaaatgaag cttgatctat gcttcattta cttcattcaa tcaaacattt
     3001 aagaaagggg aattctaatg actaaaaata aaatgacact aaaagcagag gttcttctgt
     3061 atattcaaga acatttttct aatcaagcct tttttacaaa accaatttac ttggctttcg
     3121 aaataagagg ggtatctgct ggatctattg gaggtactct tcaagcgttg aagaacgaag
     3181 gatatttaga aaatcacttt gtgcaacgtt cgttcaatgg aagagtcgcg aaagaatggt
     3241 atttagttca ttcatagaga ggtgattcaa gcatgcgtgt ggcttgttat gatcgaacgt
     3301 ctgtttatga agaagcgaaa atcagtaatg acgctcaata tgatgcagtg aaagagtatg
     3361 ttttagagaa atatgaacaa attcctcaac gattttcaga caaaggagaa tcaggtggaa
     3421 aagcggatcg tccagctttt cgacagctta gagcttgggt ttgtgcgtca gaagaagagg
     3481 gggtgatcta tgtctggcgt tatgatcgat tggcacgtca agccaaaata gccttagaat
     3541 ttattgagtt gtgccaacaa catcaggttc aagtttattc gatttctgaa cctcttcctg
     3601 cttctttctc gcacgcgtca acgaataaac tatttgtgca attattgttc acttttgcag
     3661 aacaacaacg aaacgtcatt attgaaaatg ttcgtgccgg acttgcctat aaaaagcaac
     3721 aacggcaata tttgtcttct gcggttccat atgggtatcg tttagtgaat gggaaaatcc
     3781 aacaagaagc acatgaagca aaagttgtcc gccatatctt tcaactttat ttgactaaaa
     3841 aatatggcta caaaaagctt tgtcaacgat tgacgcaaca aaagttcttc ttccgtgaac
     3901 gtccttttca gccctatcat atttatagta tcctaaaaaa tccattgtac tatggcgaaa
     3961 tcaaaggagg atctttgggg aagtatctag gaacctttga accgattctc tcgaaaacaa
     4021 tctttttaca agcccaagag attcgccaat caaggtgtac agctaaaaaa gatacgtatc
     4081 cgtacttatt acgtcaaaaa attcggtgtc ccttctgtgg tcgtcatctg agttcgaagt
     4141 atcaatggaa tacaaagaag acgaagacgc ttcattatta tcattgtacc gaccgaagct
     4201 gccaaggaat ctttcttcgg gcagaaaggg ttgagtcaga agtattggaa aagatacagg
     4261 ctttttttca atacgaaggc gtctatcaag ccttgattgc ccaattgacg caacaattag
     4321 ccgaaaagcg aaaagatgaa aaacacaaag taatccaaca aaagaaacaa gaagcacaat
     4381 ggctcaaggc atttgaagag ggcaattgtt cactggaaga actcaagcaa cagctgcaag
     4441 ctcttcaaaa gccgaggtct acttcttcgt ttctttcact ccaacagtgt gaagcaaaag
     4501 tgaaagaatg gcttcaacta aaagacctct ctagtcgaca attgttatgt gaccatgttt
     4561 gtcggatcga ggtaacaaag gaaaaaacag tctgtgggat ttacttccga caaatgacac
     4621 aaaatatttt agaagggaac gaatgatcta tggaaacgac accaaccaaa cgagtcgcat
     4681 tgtatattcg ggtgagtacc caagaacaag ctttagaagg acatagtatt caagcacaag
     4741 aacaaaatgg acgacagttt gccgaacgga tgagctatga agtggtagaa gtttatgccg
     4801 atgaagggat tagtgggaaa tccacaaaaa atcggttcgc ctatcaacga atgatgaacg
     4861 atgctcggaa ccatcgtttt gatctactag tgatttggaa attgacccgt ctcggacgca
     4921 atatgttaga tgtcttacaa accgtcgagg aactgttgcg atatgatgtg gggttacatt
     4981 cgatctcgga acaatttgac gtgactactt cgacaggaaa actaatgtta caactccttg
     5041 ggagtttcgg tgaatttgaa cgcaaccaaa ttgcggagaa tgttcagatg tcaatgcaaa
     5101 gcttagtgaa agataaaaag cgttatgcag gtggacgacg tttaggctat gtcagtggta
     5161 ttgacacgaa tggtcaaaaa caattggtca ttgaaccaga agaggcgaaa attgttcgtc
     5221 tgatctatgc aaaatattta ggcggagcag ggtatcgagc gattgccaat tttttgaatc
     5281 gtcaaggata tcaaacagtc aaaaagaatg ctttttctac cacggcagtc aaagatatct
     5341 tacgcaataa aatttatgca gggtatttag aatatgcacg ctatatcaat tgggaaacca
     5401 aacgacgaaa gggcaagaac ccaaaaccga ttttagtgaa aggtacccat gaaccgatta
     5461 tttcggaaga agattatcaa aaagtgcaag aacgccttgt tttagaaggc aaacagccca
     5521 aatggaacca tacaggtgaa aatgtattga caggcttgtt acgttgtcca gaatgtgggg
     5581 caccgatggc agcgagtaat gtcacaaata ctctaaagga tggcacaaag aaacgaattc
     5641 ggtattattc ttgttcagtc tttcggaata aaggagcaag tgtgtgtcat gccaattcga
     5701 ttcgtgccga acaagcggaa acatttgtcg ccgaccgatt aaaggagatc gttcagctac
     5761 cagaagttct tccccgattg gtggcagcgt taaatgaaga aatagtgcga caaagccaac
     5821 cattggaaca agaactcgtg gtgttactgg aacgaaagga agaattgaag actaaaattg
     5881 aaaaatggga agctgctttg gaagatagtc ctgaattgtt tccgatgtta aaagaccgct
     5941 tagacgaatt gactgaaaaa cgacgacagt tgcacattcg agaaaatgaa atcttaggga
     6001 tctttcaaca acaaggagag ccgattcaag tcaaagatgt ccaacggata ttgactagtt
     6061 tggatcgttt tcttgcccag agtgagaaga agcaagtcaa agccttgtat cgtaccttta
     6121 ttgagaaaat aacctttgat cctaactata aggagatatt ggagattaca atgaaattca
     6181 cgcctgccgt ggtacaacaa ttgaatgagc aatatcaaat agccgtatcc aaaacaaagg
     6241 atacggctat ttttgtgtta aagacaccct ttacgttaac gatctagtct aggttatatc
     6301 aaacaacgtt agaggggcgc taacgcttct ctagcgattt gaaatcgtta gatgagtcgt
     6361 tagggaggtc cttatcataa atagggatat ttattaatga atatttgaaa tataattgta
     6421 tataagaatt aaaaagagtg gggatagata tgaaaaagat gggtaagtgt attttatgtg
     6481 aaaaatacac tgaacttact aaagaacatg taccacctaa aaaatcaggg aatactggag
     6541 gtaaaaagac cagaactggt aatttagatg atttccttaa gagcgatttt acaaaaggtg
     6601 attttccgaa ggggattaaa aggaaacctc aaggaaatgt ttattatacg ttatgtagta
     6661 aatgtaatag tttttttggt agtgagtatg ttgaggaata tattagattt gcagaagaca
     6721 ataaaaattt cctttataac aatacaagtt taaagaatgg taagtctgat ttaacacatt
     6781 caataaagaa aatgaattca cttagagttg caaaagcaat tgtagcaatg ttttttagtt
     6841 taaatgggga tgaagattca atggataagc cattcttaga ttcggtacga gaatatctta
     6901 gcaatcctaa aagtacttta tttcctcatg aagactacaa aattataatg aattattatc
     6961 ttccaatagg tgctccacag aattctaaaa tcaaaattcc taatgacatt gtaaatgatg
     7021 gtgacactca caatgtaata aaaggtttta tgccccttga tctatcaact gggagaaaaa
     7081 ttcgattttc agaaattcag tatgaaggaa ttggttttac tcttatagat ttaaaaaatt
     7141 caacttttga aataaaagaa ggattagatt taaaaaatct cctttgtgca gatgataaaa
     7201 tcaaaaaaat acaattgtac aaag
//
LOCUS       DAYYGO010000122         6769 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000122 DAYYGO010000000
VERSION     DAYYGO010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.125"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1729)
                     /gene="pglX"
                     /locus_tag="ACHA5T_002406"
     CDS             complement(<1..1729)
                     /gene="pglX"
                     /locus_tag="ACHA5T_002406"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HFM8887947.1"
                     /translation="MDKKAIKNFAVNARRKLIDEVMTKAKRLGIDESKIEDVKKIDSN
                     LQEIEKSGIRIQGKEIAQRNKLIAELKQRASVTSYSEAFQELIEEVAYTWFNRLIAIR
                     FMEVNDYLPDTYRVLSSEISGKIEPDIITDIYDADIFEELSSNEQQQVRTWKTDNSAE
                     AMDKLYQLIFIKICNQLNDYLPELFEEVEDYTELLFTASYIKKDGVIADLLSIPEDDF
                     YVQAGGQVEIIGWLYQYYNSEPHNQVVNIYKGIVKKQDIPAATQLFTTDWVVRYMVDN
                     SLGKYWLDHYPNSELRNKLKYLIPDKYAFEQATSVSNLKPEELKIIDNAMGSGHILVY
                     AFDVLMEIYLTLGYSERDAARAIVEYNLYGLEIDKRAYQLSYFAIMMKGRQYDRLFLK
                     KSLSPNLREFIDSPKVTDEYQERISELNNDKYSERWNEYKELIFDQFENGKELGSIIK
                     LVDKNNQAITKEYLGKVREYISKFNEFTNMDILYGMKEIQEKYLHILDVAEMLVDKYV
                     AVVTNPPYLNKMDKKLKKYVNDNYSDVKKDLFSVFIKLNSQMLIDNGYASFMTPFVWM
                     FISSYEKLRS"
     gene            complement(1793..5464)
                     /gene="brxC"
                     /locus_tag="ACHA5T_002407"
     CDS             complement(1793..5464)
                     /gene="brxC"
                     /locus_tag="ACHA5T_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HFM8887948.1"
                     /translation="MLIKDIFAKPIDRNIQGVIKVGQAKDENVQQELEEYVVTKELQK
                     HFKTIFDAYQRSINTPTDKMGVWIQGFFGSGKSHFLKIISYLLANREINDKKAIDYFK
                     EDGKIKDPLTLATMERCSQINTDVILFNIDSKSDAGGKKDKNGIVNVFLKVFNEMQGL
                     STIPHLADFERRLIKEEKFDAFKAAFEEINGAKWQEERHEFDWIQDDIKEAALKIDFM
                     SEAAIDNFLEKASGNYLISIDDFANLVKSYLDSKEADHHIAFLVDEVGQYIGGNRELT
                     LQLQTVVEDLGRICEGKAWVLVTSQEAIDHVTNFDNNSDNDFSKIKDRFATTVSLSSA
                     NADEVIKERILKKKEFANDSLALQFETVQTELKNKLLFVDSPELKLFQDAENYAEVYP
                     FIPYQFNLLGQILTEIRNHSYQGSNLSSGERSLLAMFKEAAERNKENDTTSLVSLDQF
                     YYSLERWIDTAISRVITQATNNSHIQRPTDIDNFNVNVLRILFMIKYVDQAIKPNVEN
                     ITSLMIRNINEDRLETKKRVEEALDLLIKENFVRKNLDSYIFQTDEEQEITREIKNQN
                     ITDSDLIVELSKRIFDGVLDRNSFAYEKLGMSGGNKFKNRYTFTYAQVLDQRPYKQAG
                     NAEMTVNFISPYSDLAGQEAELALKSSRNLLFVELPSDKEFKDDIAMYARMNLYLTTT
                     YSSVVKNFDAIRSEKSGELQELSASINDQLNELLKSAKFFYNGAEIQLTGSDFKAKFQ
                     SALQKMAEEVYHKLNQIDAPKEKEDILYLVKESKNEIIEDPSNEQAVQEIFNFINDQK
                     RNHLTITMKTIRDRFETKQPYGFTELDVEWCIAKLFMSGKIDLLYNNVQISRMDDPNS
                     IVMIIQKRRESEKIKIEPSAVISPKAKKLVDEIGRELFQSRNIVDENNNEHTMENFKK
                     YLKGQLDNLRSYFDLQNTYTYPSAYEYEQIKKYLEVIVQTSDVEDFFYTIKKYRDELF
                     DWAETYDKIKNFHKQNSTQKKIWENAQDKIRLIEKSRRQTSSQEIDQLIHEMKTILKK
                     ADPYSDMMKLNELIQEFIEQYNQLLELESQEILNKLTEEKQATFKLLEGKDFKDQFVP
                     IVDQEFQELKAKIEQANTIDDLVFLSLNVAEQYLIFNQRFDNEENRRRQEEQARKQIE
                     AANLIRETEKDEIDSAAKAEEIAIVKPTIQQKTVPMRTLNVHVKTIKSAADIDELTAE
                     INQKLKEQLQENTEITIQF"
     gene            complement(5489..6064)
                     /locus_tag="ACHA5T_002408"
     CDS             complement(5489..6064)
                     /locus_tag="ACHA5T_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HFM8887949.1"
                     /translation="MSLSIQEHLEKLNEVIQKEDFLLNRGLGNEVGYYIFDYDPKDEL
                     IVRDYINDISGTSVGDNYEVKVYNLYVIMLEYLEKRGFTEKAIKMEERRGLQKLVESI
                     GRLLKMDLPEEENVFFEYVDAHTPEKSVVFLTGIGELYPLIRAHSILNKMHQVFDRVP
                     VVMMYPGEYDELSLTILNTNKDDNYYRAFRI"
     gene            complement(6064..6663)
                     /locus_tag="ACHA5T_002409"
     CDS             complement(6064..6663)
                     /locus_tag="ACHA5T_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HFM8887950.1"
                     /translation="MNKKYSAGLVSKRFWFSEFKLYIQLRLEGYTDIEIKEKNEHENI
                     FFAVSSNRQKEIYQNVKRRVSQLDEAGLQLFNQLNIDNQKLVNLISILMIDDLFNEFM
                     TEVYAENLRKGNLQLTPVDYRSFFTEKRRSSEDISKWKDYTIKRLIAAYQTNLIETGL
                     VRGEKEYGRMTPILLDKRLIQWLVDKNRKDIVLALGGIL"
BASE COUNT         2004 a         1278 c          901 g         2586 t
ORIGIN      
        1 atgagcgtaa cttttcatag ctagaaatga acatccaaac aaatggcgtc ataaaggaag
       61 catatccatt atcaatcagc atttggctat tcaatttaat aaacacagaa aatagatctt
      121 ttttgacatc agaatagtta tcatttacat atttttttaa tttcttatcc attttattaa
      181 gataaggcgg attagtgact accgctacat atttatcaac tagcatttca gccacgtcaa
      241 gaatgtgcaa gtatttttct tgtatttcct tcatcccata aagaatatcc atattcgtaa
      301 attcgttgaa ttttgatata tactctctta cttttcctaa gtattcttta gttattgcct
      361 gattattttt atcgactaac ttaatgattg atccaagttc tttcccattt tcaaattgat
      421 caaaaattaa ctctttgtat tcattccagc gttcactata cttatcattg ttcaattcac
      481 taatacgctc ttgatactcg tctgtcactt taggagaatc aataaactct cttaaattcg
      541 gcgatagact ttttttcaaa aaaagacgat cgtattgacg ccctttcatc ataattgcaa
      601 aataagacaa ttgatacgct cttttatcga tttcaagacc atataaattg tattcaacga
      661 ttgcacgtgc agcatcacgc tcagaatatc ctaacgtaag ataaatttcc atcaatacat
      721 cgaaggcgta aactaaaata tgcccagacc ccattgcgtt atcgataatt tttagttctt
      781 ctggttttaa attacttaca gatgttgctt gttcaaaagc atatttatct ggaattaagt
      841 atttcaactt atttcttaat tcactattgg gataatgatc taaccaatac tttcctaaac
      901 tattgtcaac catataacga acgacccaat ctgtggtaaa tagttgagtt gctgccggaa
      961 tatcttgttt tttgacaatc cctttataaa tattcaccac ttgattatgt ggctcactat
     1021 tatagtactg atacaaccac ccaataatct ctacctgtcc accagcttga acatagaaat
     1081 catcctctgg aatacttaaa agatcagcga ttacaccatc ttttttaata taagaagctg
     1141 taaacaaaag ttctgtataa tcttctactt cttcaaatag ctcaggtaag taatcattca
     1201 attgattaca gatttttata aaaatcaatt gatacagctt gtccattgct tctgctgaat
     1261 tatctgtttt ccaagttctg acttgttgct gttcattact agataattct tcaaagatat
     1321 cggcatcata aatatctgta ataatatctg gctcaatttt cccacttatt tcactagata
     1381 atacacgata cgtgtcaggt aaataatcat ttacctccat aaaccgaatg gcaatcaatc
     1441 ggttaaacca cgtataagca acctcttcga ttaactcttg aaaagcttct gaataagaag
     1501 tgacagaagc acgttgtttt agttctgcaa tcaatttgtt tctttgcgct atttcttttc
     1561 cttgaattct tattcctgat ttttcgattt cttgtaaatt gctatcaatt ttcttcacat
     1621 cttcaatttt tgattcatca attcctaaac gttttgcttt agtcattact tcatcaatta
     1681 attttctacg ggcattgacc gcgaaattct ttatcgcttt cttatccatt tttctttccc
     1741 ttctatatgt aacctttgaa aagacaggca cctttttatg cctgtctttt cactagaatt
     1801 gaatcgtgat ttctgtattt tcttgaagtt gttccttcag tttttgatta atttcagctg
     1861 ttaactcatc aatatctgct gctgacttaa tagttttaac atgaacgtta agtgttctca
     1921 ttggtactgt tttctgttga atagtaggtt ttactatcgc tatttcttca gcttttgctg
     1981 cagagtctat ttcatctttt tcagtttcac gaatcaagtt tgctgcttca atttgtttac
     2041 gtgcttgttc ttcttgacgt ctgcggtttt cttcattatc gaaacgttga ttaaagatca
     2101 aatactgttc tgctacgttt aatgataaga aaacaagatc atctatagta ttcgcttgtt
     2161 caatttttgc tttcagttct tgaaattctt ggtcaactat tggtacaaat tgatccttaa
     2221 aatcttttcc ttctaataat ttaaatgttg cttgtttttc ttccgttaat ttatttaata
     2281 tttcctgact ttccaactct aacagttgat tatattgttc aataaattct tgaatcaact
     2341 catttagttt catcatatct gaataaggat cagccttttt caaaatggtt ttcatctcat
     2401 gaatcaattg atcaatttct tggctagaag tttgacgccg acttttttcg attaatctaa
     2461 ttttatcttg tgcattctcc cagatttttt tctgagtact attttgcttg tggaaatttt
     2521 taattttatc atatgtttca gcccagtcga ataattcatc gcgatatttt ttgattgtat
     2581 aaaaaaagtc ttcaacatct gacgtttgaa caattacttc aagatatttt tttatttgct
     2641 catattcata agcactaggg taggtataag tgttttgtaa atcaaaataa gagcgtaaat
     2701 tgtctaattg accttttaaa tatttcttga aattttccat tgtatgttca ttgttatttt
     2761 catcaacaat attacgactt tgaaacagtt ctctaccgat ttcgtcaacc aatttttttg
     2821 cttttggcga aatcaccgcg cttggttcaa tttttatctt ttcactttct cgtcgttttt
     2881 gaataatcat tacaatacta tttggatcat ccatccgtga aatttgtaca ttgttataaa
     2941 gtaaatcaat tttaccagac atgaaaagtt tagcgatgca ccattccaca tcaagttctg
     3001 tgaatccata cggttgtttt gtttcaaaac gatcacgaat tgttttcatc gtaatcgtta
     3061 ggtgatttct cttttgatca ttgataaaat tgaaaatttc ctgaactgct tgctcatttg
     3121 atggatcttc aataatttca tttttgcttt ctttcactaa atataagata tcttcttttt
     3181 ctttaggcgc atcaatttgg tttaatttat ggtatacttc ttcagccatt ttctgaagtg
     3241 ctgattggaa ttttgcttta aagtcggagc ctgtcagttg aatttctgca ccgttataga
     3301 aaaattttgc agatttcaac agttcattta attgatcgtt gatacttgca cttagttcct
     3361 gtaattcacc agatttttcc gaacgaatcg cgtcaaagtt ctttactaca ctcgaataag
     3421 tcgttgttaa ataaagattc attctagcat acattgctat atcatcttta aattctttat
     3481 ctgatggcaa ttctacgaat aataagttac gggaactctt aagcgcaagt tctgcctctt
     3541 gacctgctaa atcagagtat ggtgaaataa aattaactgt catttcagca tttccagctt
     3601 gcttgtatgg acgttgatct aacacttgtg cataagtaaa cgtataccgg tttttaaatt
     3661 tatttccacc tgacatgcct aatttttcat aggcaaaact atttcggtct agaacgccgt
     3721 caaaaattcg tttagatagt tctacgatca aatcactatc tgtaatattt tgatttttaa
     3781 tctcacgagt aatttcctgt tcttcatcag tttggaaaat ataactatct aagttcttcc
     3841 gcacaaagtt ttccttaatt aaaaggtcca atgcttcttc tactcgtttt ttagtttcca
     3901 ggcggtcttc gttgatattt cttatcatca agctcgtaat attttcaaca tttggtttga
     3961 tggcttgatc aacatattta atcatgaata aaattcgtaa gacatttacg ttaaagttat
     4021 ctatatcagt tggtctttga atatgagaat tatttgtagc ttgtgtaatc actcggctaa
     4081 ttgctgtatc aatccaacgt tctaacgagt aataaaactg atctaaagaa accaaactgg
     4141 ttgtatcatt ttccttattt cgttcagcgg cttctttaaa cattgctagt aatgaacgtt
     4201 caccagacga taagtttgat ccttgatagc tgtgattacg aatctcggtt agaatttgcc
     4261 ccaataaatt gaattgatag ggaataaatg gataaacttc tgcgtaattt tcagcatctt
     4321 ggaagagctt taattcagga ctatcaacaa agagtaattt atttttcaat tctgtttgaa
     4381 cagtttcaaa ttgtaaggct aaagaatcat tggcaaactc ttttttcttc aaaatacgtt
     4441 ctttaattac ttcatcggca tttgctgaag ataaactcac agttgtggca aaacgatcct
     4501 tgatcttaga aaaatcatta tcagaattgt tatcaaagtt tgttacgtga tcaatcgctt
     4561 cctgacttgt cactaatacc caagccttac cttcacaaat tcgacccaaa tcttccacaa
     4621 cggtttgtaa ctgcaaagtc aactcacgat taccgccgat atattgtccg acctcatcga
     4681 ctagaaaggc aatatgatgg tctgcttctt tggaatcaag atagctttta actagatttg
     4741 caaagtcatc aatgcttatt aaataatttc ctgatgcttt ttctaaaaaa ttatcaatag
     4801 cggcttcact cataaaatcg attttaagtg ccgcttcttt gatatcatct tggatccaat
     4861 caaactcatg gcgctcttct tgccattttg ccccattaat ctcttcaaaa gcagctttga
     4921 atgcatcaaa cttttcttct ttaattaacc gacgttcaaa atctgctaga tgtggaatcg
     4981 tgcttaatcc ttgcatttcg ttaaatacct ttaaaaaaac attcacgatt ccgtttttgt
     5041 cttttttacc acctgcatca ctttttgaat caatattaaa cagaataaca tctgtattta
     5101 tttgtgagca ccgttccatc gttgccaaag tcagtggatc tttgattttc ccatcttctt
     5161 tgaagtaatc aatggctttc ttgtcattta tttctcgatt tgcgagcaag taagaaataa
     5221 tttttaagaa atgagattta cctgaaccaa agaacccctg aatccaaaca cccattttat
     5281 ctgttggtgt attgatactc cgttggtagg catcaaaaat tgttttaaag tgtttttgta
     5341 gttctttcgt aaccacgtat tcttctaatt cttgttgaac attttcatcc tttgcttgac
     5401 cgaccttaat aactccttga atatttcggt caattggttt tgcaaaaata tctttaatta
     5461 gcatttaatc tcctaccttc catcccaact aaattctaaa tgctcgataa taattatcat
     5521 ctttattggt attaagaatt gtcaaactta attcatcata ttcccctgga tacatcatca
     5581 caactggtac gcgatcaaat acctgatgca ttttatttaa aatagagtgc gctctaatta
     5641 atggatataa ttcgccaatt cctgtcagga acacaactga tttttctggt gtatgggcat
     5701 ccacatattc aaaaaataca ttttcttcct caggtaaatc cattttcaat aaacgaccaa
     5761 tgctttccac taatttttgt aaaccacgtc gttcttccat cttaatcgct ttttcagtga
     5821 aaccgcgttt ttctagatac tctaacataa tcacatagag attataaact ttcacttcat
     5881 aattatcacc aacagatgtt ccagatatat cattaatata atctcttaca attaactcgt
     5941 ctttcggatc gtagtcaaag atataatagc ctacttcatt acctaaacca cgattcaata
     6001 aaaaatcttc tttttgaatt acttcattca acttttccaa atgctcttga atcgataatg
     6061 acattataaa attcctccca gtgctagtac aatatcttta cgattcttgt caactaacca
     6121 ttgaatcaat cgtttatcta ataatatcgg agtcattcgt ccatattctt tttcccctct
     6181 gactaatcct gtttcaatta gattcgtttg ataagcggca ataagccgtt taatcgtata
     6241 atctttccat tttgaaatat cttctgaact tctccgcttc tctgtaaaaa aagaacggta
     6301 atcaactggt gttaattgca aatttccttt acgtagattt tcagcatata cctcagtcat
     6361 aaattcatta aaaagatcat caatcataag gatggaaatc aaattcacta acttttgatt
     6421 atctatattt aactgattga aaagttgcaa tccagcttca tccagttgac taactctcct
     6481 tttcacgttc tgatagattt ctttttgacg gttactgctc actgcaaaaa aaatattttc
     6541 atgttcattt ttctctttaa tttctatatc tgtatatcct tctagtcgta attgaatata
     6601 taacttgaac tctgaaaacc aaaagcgttt agaaactaga ccagcagaat attttttatt
     6661 caatagattg cctcctaatc atctataatt caataagtaa acaagtgttt tttgctcatc
     6721 gttagcattc attttttagt atctactata caatacatat ttattatag
//
LOCUS       DAYYGO010000123         6716 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000123 DAYYGO010000000
VERSION     DAYYGO010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6716
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.126"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(2..1768)
                     /locus_tag="ACHA5T_002410"
     CDS             complement(2..1768)
                     /locus_tag="ACHA5T_002410"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8887951.1"
                     /translation="MQMIGGLTMKSFYKNKFALTNQGAEDLVKASISSFFVYCINMVP
                     AFIIMMLIDELVLENVKPPWLYFTVSFVTLLFMYWLLDREYENLYNSTYKESAHLRMQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKIVDQGTHSELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1728..3482)
                     /locus_tag="ACHA5T_002411"
     CDS             complement(1728..3482)
                     /locus_tag="ACHA5T_002411"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8887952.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLK
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3592..4161)
                     /locus_tag="ACHA5T_002412"
     CDS             complement(3592..4161)
                     /locus_tag="ACHA5T_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFM8887953.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(4179..5594)
                     /locus_tag="ACHA5T_002413"
     CDS             complement(4179..5594)
                     /locus_tag="ACHA5T_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8887954.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            complement(5605..6273)
                     /locus_tag="ACHA5T_002414"
     CDS             complement(5605..6273)
                     /locus_tag="ACHA5T_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFM8887955.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(6404..>6716)
                     /locus_tag="ACHA5T_002415"
     CDS             complement(6404..>6716)
                     /locus_tag="ACHA5T_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8887956.1"
                     /translation="IFENKRIFDLVLFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKT
                     IGMINQNLVFNEEEIHFYIYSFYATFYDILKHSYSKEKTMLLTRRLYQFSVPGWIELL
                     S"
BASE COUNT         2284 a         1229 c          899 g         2304 t
ORIGIN      
        1 atcatcgtga ttgagcttct ttttcataga cgaatttctc agatagttta gttttcttta
       61 tgagattttt gtatatttct gaccttcgat acaactcact atgagtacct tgatcaacta
      121 tttttccttg atccatcact acaattttat ttaccttttc aatagacttc aagcgatgcg
      181 atataattag tactgtctta tcttgtatta aacgatttaa actttcctga atacgttttt
      241 cattttccgc atctaaactg gcagtaattt catctaaaat taaaattggc gcatttttca
      301 aaaaagcccg tgctatggat aggcgttgtc gctctcctcc agaaagtgaa cttccgtttt
      361 ctccgaccaa tgtatgataa ccgtcaggta gtttttcaat aaagtcttcg cagttagcta
      421 aacgagctgc ttttttcact tcctcatctg acgcttgact atttccaata cgtatatttt
      481 ccaaaatact tgtattaaac aaagttacct cttgaaaaac aatagcgatt ttgctaaaca
      541 aagaagctgg agatacacgt ttaatatcat acccatcaat taaaatacag ccttcatcat
      601 aatcatacag tctagacact aatttcaaga tactagtttt cccggaacca ctagcaccta
      661 ctaacgcagt cacttctcct tgtttagccg taaaagagat atgatctaaa attggggtat
      721 tgttatcata tgagaaagag acatctcgca actcaatgtc aaaagatttc agaggactat
      781 ctgatccctc ttggatactg gtttccttca tagctctaat tcgctgtatt ttaggagcta
      841 aataaaatat ttcaagcact gcttctttca tagaatcgaa ggaatccttt atttttattg
      901 cggctagtaa gtaaccaacg acgtagagta tagtcacttc acccgttaac agtaagtgaa
      961 cacctactag aatgactact gctaaactaa catatgagaa aatagaggat aaggccatga
     1021 ctgaaaaagt acttaattct acctttaaat gaatcctttc actctcttcc atttttttga
     1081 aaagtctgtc ttgaactttt ttagatagat taaagctatt aatctcttgc tgcaattcta
     1141 tagtttcttg aaattcttcc gagttttctc tcaaagtatc gtaatattta gtattggctt
     1201 tcgtttggga tttctttgat aacaagatta agacaaaact aaataacgtt ggcaaaataa
     1261 cagctaatcc cattttgaca ttaccaacca aaagcatcac tgaaataaaa gggaaaaaca
     1321 gagccatacc gccggcttta ggtattgcat gactcatcgc atgctcaata ccttcaacgt
     1381 cagacatgat agtttgagat aaatctgata aattatgttt tgaaaaatag gataatggta
     1441 aatttgacaa atcgtctgca atttgcattc ttaaatgcgc actttcttta taagtactat
     1501 tatataagtt ttcatactcc ctatctaaca accaatacat gaaaagcaaa gtaacgaacg
     1561 atactgtaaa ataaagccaa ggcggcttta cattttctaa aactaattcg tctatcagca
     1621 tcattataat gaatgctggt accatattaa tgcagtatac aaaaaagcta gaaatagatg
     1681 ctttaaccaa atcttctgcg ccctgattag ttaaagcgaa tttgttttta taaaaacttt
     1741 tcattgtcaa gcctccaatc atttgcattc tcataattag tgaccaattg tttatacact
     1801 tgatgtttag ccattaattc attatgattt ccacgttcta ctattttccc ttctgacaag
     1861 acaagaattt catctacaga tttaatgctg gataatcgat gagctatcat aataactgtt
     1921 ttttctttca tcagatgttg aaaagccttt tgtagttcat actcattatc tggatcaata
     1981 gatgcactgg cttcatccat aataacaatc tttgcatctt ttaaaattgc tctggcaata
     2041 gctatacgtt gtttttctcc tcctgacaga taaactcctt tactaccaat taaggtatgc
     2101 tctctttcgg gaaatttttc taaaatcgat tcgcaaccgg ctaatcttag cgcgtcaaat
     2161 actttacttg ctggcgcttg aggatttgct atagaaacgt tatcgtaaat actctgattg
     2221 aatagtttcg tatcttgaaa gacaaaagca attgtgttaa taattgtgtc ttttgaatag
     2281 tgatttaaag gcttaccacc aattttaatc tgtcccttat ccacattgta gaaacctgaa
     2341 attagtttag ctatcgtact ctttccgctt cctgattttc ctactaatgc ataaattctt
     2401 ttttgttcta aaactaagtt gaaattttct aaaacacact cttctttgta tgaaaagctg
     2461 acattttcaa atgcaatgtc acaattttta atctgtgtct cgttcccata ttttagttgg
     2521 tcttgcttca tttctaaata aagggagtct agtgtttcga ctgcatatct tgcttcaaac
     2581 aagtgcatac ttacatacat tattctcatg aaagaaacaa aaataattcc acttaaaaaa
     2641 aacaacatca acaagtcaac agctaatatc ttgggagtgg taagagaagt tataaaaaag
     2701 gttataggaa ctatcaaaat gcaaactatg ccaaaaaata gccattgaaa aagaacaaaa
     2761 ggtgttttcc cactcaaaga atacttatat gcaaacttag cataatcgtt aattgcttta
     2821 ttcaaggctt taaatgatgt tactttagca ccaaatatct ttataacttg aattcctcgg
     2881 atatattcta ccgtttcaga gcttaacaca tctaacgatt cttgatattt ttgaataaaa
     2941 tttgtattct ctgtcatttt ctttaacaag aatacaccaa ttactaacat ggcgattaga
     3001 accaatccaa ttttaaaatt aatgaaaaag cctaaggcaa ctgataaaac aggtgttact
     3061 atggcttgag cactatctgg aatcaagtga gcaattgcca tatgtgtttt agccgcgttg
     3121 tcatcaattg tctttcgaat atatcccgaa gaatttaaat caaaaaaacg aaaacttgct
     3181 tctgttaaac cgctaattcc cttttttctc aaattagtct ctaagcgaaa cccaaggata
     3241 tgagaaaata agccagaaaa aatataaaat aaagctccta aagttaacca agcaacagtt
     3301 tgaaccgcat aactcttagc taaatattca tctcctacaa taattagaga cttaagaaat
     3361 ttgtaaatac tgtaatatcc atagactgtt aatacgacag aaaaatcaga aaataaagtg
     3421 gctaaccatc cgtagtattt cattttaggg acataactaa ataatttttt gtatactttc
     3481 attttttcct cccccgcatt caatttcttc tcttattcgt ttacaactgt taatgctttc
     3541 cgtaatagcg taaattagag atgtacaaac agatagaagg taaactacgt tctatttttt
     3601 gtattttttt aagacttttc ttccaatcaa tacaccaatc atagataaac ttgctacaac
     3661 taacactgaa atgattcccc atatacctac cgcatagtgg atcattgcat ccattgttgc
     3721 agcagattgt cctttatcta tccaatcttg acgaaaacga tctgtaaaga accatatagg
     3781 aatccactgt ccggcagcac tgcctaattg cattagagcc caagacgcaa cttgaggaat
     3841 tgctcgatca tatccaaata cagatagaat aatatcagct aagatcgcca tactaatcga
     3901 actgataaac caaggtaagt acgccccgcc cattaacgta aaaaataaca ataaaacaat
     3961 tgtgtaaata gtaaaaattc cttttctagg gtagttatag ctaatataaa caaatatgat
     4021 tccagataat aatccaaaaa tccctgggga tatgctgtga ccaaacgccc caccaatact
     4081 actaaccatt attgaaaaaa agtaaattac tagggaaata actgtagcta aaccaataaa
     4141 aatataatct tttgttttca tatatattct cctcaattct atttcgttat gtcattaaaa
     4201 atctgttcta tcaaagttga atcttctact ctaagtttta tatctttttt tattccacca
     4261 ttttttaaat aaataattct attgcatgtt ttataaagaa attccggatc atgagtggct
     4321 ataactactg cacccttttc ttttaagtct ttaataaggt cagcaatcct ttccatatta
     4381 atataatcca acccacttgt aggttcatca aatagaaata aattagtttt tgacaatgaa
     4441 gctatagaaa ccaataatct ttgtttttct ccaccggata aatcaaacgg taattgatct
     4501 ttcattttat aaagttccat tttttttaaa attttttctt gctgatcttt actaactatt
     4561 tcattcccca gggctaactc gctcctcact gattctgtga aaaattggta atccatttct
     4621 tgcattacta aaaacggcga tgacaaattt tttttatttt cgattatctt tcctttggta
     4681 gggcgaataa tcttcatgag tattcttagt aaggttgttt tccccacacc gtttttccct
     4741 accaaaccaa taacatctcc aactttgaca tcaaaattta tgtcttttag aatattttca
     4801 taagtaattt cttcaacttt taatacatct gcacttacct tttcgatata gggaacttca
     4861 atcgaactat taaaaatgtc aaatttcctt aatgcgttat tgttttcagt ttttaacaac
     4921 tgaatagttg tagtttttac agttctattt ttaacaaata gaacttgatc aattaattct
     4981 ttcaaatagt aaaaacgatg ttcagctata acaatagtaa atcctttatt ttttaaatca
     5041 caaagtaagt cagcaagttt cttagtcgct ttgaagtcca aatttgaaga cggttcatcc
     5101 aatataatta atttagagtt tagcgataaa ctacatccaa tagaaatttt ctgccgttca
     5161 ccacttgata gcgaaaaaat gtttttatct aatatatttg aaagatctaa taattcagct
     5221 aattcattga ttcttttatc catatgctct tttgtaaatc caaagttttc catcgaaaat
     5281 actaactcac tcgttgtatt tgttgtaaag aattgtcctc taggatcttg acttacgtag
     5341 ccaatatttt ttgatatact ttcttgtgtt aaggattgta gcgagttatt ttcaaataaa
     5401 atttccccaa ccaattttcc gtcataaagt cttggaataa gtccattcat taactttaat
     5461 agtgtggatt tcccactccc agaagaacca gtaatcaatg taagactacc tttttttatt
     5521 gaaaaattaa ggtcaacaag cgcttgttcc ttaacatcct tatatgaaaa agaaacattg
     5581 ttaaattcaa acaattcttt cacctcaaat caaaatactt aagaaaaaaa tagtaacgga
     5641 aaacaggatg atatagtcta ataagctcat gtttgtttta tagtaagaac ttcttttcac
     5701 aggagattct atcccctttg ttattgcatt agcggtcatt tcttcagcaa tcatagttgc
     5761 acgtataaga acgggaacta acagaaattc caaatattgc agaggctgaa aaaaagacaa
     5821 gcgaatgttt ctcattttta aagattcttt aatcattctt atttcagaac gataagtcgg
     5881 aaagaatctg atcactactg taatacttaa cattattttt ttcggtaaat gtattttttc
     5941 taaagaggat aataattcat tgctagtata gacactaacc aacaaccatg agatcattac
     6001 gaccggcaac attcgaaata caatatataa ggatgtccca aaaaatatag taaaaaactc
     6061 tatcttgata gcatcaataa ttttcattac taaaaacaga actgaataaa tcaagatgaa
     6121 ataaatacat gtctttattt tacgaaacat caatactaaa actgtagcaa taatgaaaca
     6181 ccccacttga aatatatttc cctttcctaa aaaaataatg accggaaaaa aaatatttaa
     6241 agtaatcaag gttaacggat tgatttttga cactctttca cctccaaaaa ataagatcgc
     6301 actgttatct taagctgata aaaatgcacc gttattcaga taacagtgcg atcttatcaa
     6361 aaagcataac tgagaacaat tctcaatgca agcttttttt taattaactc agtaattcaa
     6421 tccacccagg aacagaaaat tgatacaatc ttctggttaa tagcatagtc ttttcttttg
     6481 aatagctatg cttcaatata tcatagaatg tagcgtagaa tgagtaaatg tagaagtgga
     6541 tttcttcttc attaaagact aaattttgat tgatcattcc gatagtcttt aacttatcta
     6601 gacaacagac tgtctttttt gtcacaaatg atgtaatttt ttcaacaata tcttgatatt
     6661 tggttccagc tgattggaat aaaaccaaat cgaatatacg tttattctca aaaata
//
LOCUS       DAYYGO010000124         6709 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000124 DAYYGO010000000
VERSION     DAYYGO010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.127"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(47..871)
                     /gene="iolB"
                     /locus_tag="ACHA5T_002416"
     CDS             complement(47..871)
                     /gene="iolB"
                     /locus_tag="ACHA5T_002416"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HFM8887957.1"
                     /translation="MEKTLKHQPLNKELAPGVKQIQNIRQSNSALSYIEFQILELEKG
                     ASYKETGSQQEVCIVILTGKIKVKAADTWYEDLGTRESVFEKIPTDSIYLPAEHSFEL
                     VATTDNVRLALCYSPAKKALPISVIRAEDNTVEERGLYQNKRLVHNILPDNVDTASSL
                     LVVEVYTEGGNFSSYPPHKHDQENLPHESFLEETYYHELDPEQGFVFQRVYTEDRSLD
                     ETMTVEHQNVVEVPKGYHPVGVPDGYTSYYLNVMAGPERIWKFHNEKKHEWILQRK"
     gene            complement(910..2826)
                     /gene="iolD"
                     /locus_tag="ACHA5T_002417"
     CDS             complement(910..2826)
                     /gene="iolD"
                     /locus_tag="ACHA5T_002417"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002397168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HFM8887958.1"
                     /translation="MEKTVCLTTAQALLKFLNQQYISIDGKETPYVEGIFHVYGHGNV
                     LGIGQALELEPGHLKSYSGKNEQGMAHAATAFARQNLRKKIFAVSTSVGPGAANLLTA
                     AGTAFANNIPVLFLPADTFATRQPDPVLQQLEHASSVAFSTNDAFQAVSRYWDRINRP
                     EQLMSALLRAFEVMTNPATMGPATICLPQDVEAEAYAYPEEFFKKRVHYIDRRIPTAR
                     ELAEASQRIKASKRPVIIVGGGCKYSEAGEILEQISRDCQIPLVETHAGKSTVSWDFK
                     NNLGGMGILGTSAANKAVQQADLIIGIGTRYTDFTSASKSIFNFEQAKMMNINVSRQQ
                     AAKLEAFQVVGDAKATLEQLAPMIEGYQTAYGEKIDELKEEWISERNRLKAAHFKRKD
                     FTPEIKDHFSQEILNEYADALATNLTQTEVFIHLNDFVDEDAIVIGSAGSLPGDMQRL
                     WNPVKENTYHLEYGYSCMGYEIAGAMGVRMARPEQEVYALVGDGSFLMLHSELVSSLQ
                     YDKKINIVLFDNSGFGCINNLQMDNGGASQGTEFRNTKDEIMNIDYAKVAEGYGAKTY
                     RVSSIAELQAAVEDAKKQTRSTLIEIKVLPKTMTEGYDGSWWNVGISEVSGKAAMQEA
                     YKAKQAKLATIRKY"
     gene            complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="ACHA5T_002418"
     CDS             complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="ACHA5T_002418"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047590 - 5-dehydro-2-deoxygluconokinase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HFM8887959.1"
                     /translation="MSYIKFDQSKEFDLLLLGRVAIDFNPAYNDQVKEEFKPLKKVHM
                     FEKFVGGSPANIAVGVTKHGMKAGFIGTVSDDQFGEYVIDYFNEQGIDTSQISKAQHG
                     EKLGLTFTEMLSSKESNILMYRNQAADLQLSVEDIDEDYLKRSKALLISGTALAESPS
                     REAVIKAVMLAKRNNVKIIFDIDYRAYNWKNNDDISIYYSMIAKESDIIMGSREEFDL
                     TEKLIQPNMSDNESAAFWHSCGAEIVVIKHGMKGSTAYTADQQQFSIKPFPVEARKGF
                     GGGDGYGSGFLYGLFEGWEIIDCLEFGSAEASMMVKSNNCSDSLPTTQEVKDFITLEK
                     EIYGEMIARI"
     gene            complement(4028..5044)
                     /locus_tag="ACHA5T_002419"
     CDS             complement(4028..5044)
                     /locus_tag="ACHA5T_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016633586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8887960.1"
                     /translation="MAGTIKEIAEKAGVSRGTVDRALNDRGRINPEVAERIRAIAKEM
                     NYVPKKKKVIKETTLKLGIVTQLAKSSFMIPIRSGLESIIGDLKKRNIDCFLEEVDGV
                     DEAAQLQALDRLVELGVKGIAIMPVDSDGIREKINQLTDQGILIITFNSDILGTNRQC
                     FVGMDNFKSGKTAAGLLGMLTKGKGKVLAITGYFGNSVNSLRVAGFVEELKESFPELE
                     LTGVQSSFDSSAEVEKILSNTLENYSNLDGVVVFSGGQAGIARALEKIDESKRPFVII
                     YDLTKNNIEMLKNDQVDFLIDQDGFVQGYRSLLMLANQLQNNKTVTKDALFTDIIIKT
                     KYNI"
     gene            complement(5146..5298)
                     /locus_tag="ACHA5T_002420"
     CDS             complement(5146..5298)
                     /locus_tag="ACHA5T_002420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887961.1"
                     /translation="MDVVKFLDDADIANDPNNPPQFTCEKCGKEMYPEYYRNALGIEF
                     KISDVQ"
     gene            complement(5315..6634)
                     /locus_tag="ACHA5T_002421"
     CDS             complement(5315..6634)
                     /locus_tag="ACHA5T_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016372324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFM8887962.1"
                     /translation="MKANILEAIFFDEHHNWDNFVEKYGRKIRLVVKREVNKFRYCGD
                     IQKGYRLFVCEGCHQTKFVPLKCRGKFCPTCATGESQRWSAVVANDMFKVTHRHVIFT
                     IDEGLREIFMMENYRDSLLKGLMDEAAKIILAYFEKRKVKAGIVATIHTFGSKLEFNP
                     HVHMVVTMGGLTRDGSWEEYDYLPFSMLRKYWKNAVLKLIRRTLGEWDKQRVQSRLQA
                     AYKNNGEGFYVNAPKRSRTNLKSLLQYISRYMKRGPIALSRILMYDGDTVMFNYMDKR
                     TNTKETMTMSIDEFIAALIRHIQNKNFKTIRRYGIYSRRIKTLMKKVMKEYQKRVKKL
                     FINLNEALRPKSWEERITEEFGENPLECSCCGEYYEFVGMSVRKNGRLVIKYAKDNHA
                     KRFMREENRKIDQKEFRQQYEEEKEKTFKAIRWDWERQREIYMSKLW"
BASE COUNT         1769 a         1635 c         1158 g         2147 t
ORIGIN      
        1 acctaactaa acctaactaa actttatgta tctaacagct taataactac ttgcgctgca
       61 agatccattc gtgtttcttt tcattgtgga atttccaaat tcgctcaggt cccgccatca
      121 catttaaata gtaggaagta tacccgtcag gtaccccaac tggatgatag cccttaggaa
      181 cttcgacgac attttgatgc tctaccgtca tcgtttcatc tagtgaacga tcttccgtgt
      241 agacccgctg gaaaacaaat ccctgctctg gatcaagctc atggtaataa gtttcctcta
      301 aaaaagactc atgtggcaga ttctcttgat cgtgcttatg aggaggatag ctggaaaaat
      361 taccgccctc ggtataaact tcgacgacca gcaggctgct ggcagtatcg acattatccg
      421 gtaaaatgtt gtgaacaagt cgtttattct gatagagtcc acgttcttcg acggtattat
      481 cctccgcgcg aatcacactt atcggcaaag cttttttcgc tggtgaatag caaagcgcca
      541 atcggacatt gtctgtagtt gccacaagtt caaacgaatg ctccgccgga agataaatac
      601 tgtcggttgg aattttttca aaaacagatt ctcgtgttcc taaatcttca taccaagtgt
      661 ctgctgcttt aaccttaatt tttccagtca aaatgacgat acagacctcc tgttgactgc
      721 cggtttcctt gtaagaagct cccttttcca gctccaaaat ctgaaattcg atataactca
      781 aagcagaatt agactgccga atattttgga tttgttttac tccaggtgcc agttctttgt
      841 ttaatggttg atgcttcaat gtcttttcca atcaatccac tccttatttt caatcacaaa
      901 aagcaatatt taatactttc tgatagtggc aagtttcgct tgtttggctt tgtaagcctc
      961 ctgcatggct gctttacccg atacctctga gataccgacg ttccaccagc tgccgtcgta
     1021 gccttctgtc atggttttag gaagtacctt tatctcaatc aacgtcgatc gcgtttgctt
     1081 cttcgcatcc tcgaccgctg cttgtaattc tgcaatcgag cttacacgat acgtttttgc
     1141 accatagcct tcagcaactt tggcgtaatc gatgttcatg atctcgtcct tggtatttct
     1201 gaattcggtt ccttggctag ctccgccatt atccatctgt aaattattga tacagccgaa
     1261 accagaatta tcgaaaagta caatgttgat ttttttatca tattgcaaac tagaaaccag
     1321 ctcgctatgc aacatcaaga aactgccatc gcctactaag gcatagacct cttgttctgg
     1381 tcgagccatt cttacgccca tcgcaccagc gatttcatag cccatacaag aataaccgta
     1441 ttccaaatga taggtattct ccttcacagg gttccataag cgctgcatat cccccggcaa
     1501 tgaaccggct gaaccgatca cgatcgcatc ctcatcaaca aaatcattta aatgaatgaa
     1561 gacctctgtt tgcgtcaaat tcgtagccaa cgcatcagca tattcattca agatttcttg
     1621 agaaaaatga tctttgattt ctggcgtaaa atctttacgc ttaaaatgcg cggcttttaa
     1681 ccgatttcgc tcactgatcc attcttcctt cagttcatcg atcttctcgc cataggcggt
     1741 ttggtaacct tcgatcattg gggcaagctg ctccaaggtc gctttcgcat caccaaccac
     1801 ttgaaaggct tccagcttag cggcctgttg tctgcttacg ttaatattca tcattttcgc
     1861 ttgttcaaaa ttaaaaatac ttttagaagc tgaggtaaaa tcagtgtagc gtgtgccgat
     1921 ccctatgatc agatccgctt gctgcacagc tttattagca gccgaagttc ccaaaatccc
     1981 cattccgccg agattgtttt taaaatccca cgaaacagtc gatttacctg cgtgcgtttc
     2041 aactagcgga atctgacaat ctcttgagat ttgctcaaga atctcaccag cctccgaata
     2101 tttgcagccg cctcctacga tgatgacagg tcgtttactg gcctttattc gttgactcgc
     2161 ttcagcaagc tcacgagcgg tcggaatcct gcgatcaata taatgtactc tttttttgaa
     2221 aaattcctcc ggataggcat aggcttctgc ctccacgtcc tgcggcaaac aaatcgttgc
     2281 cggacccatc gttgccgggt tcgtcatgac ttcaaaggcc cgcaataacg cggacatcaa
     2341 ctgctctggt cgattgatgc ggtcccagta gcgagaaacc gcttgaaatg catcatttgt
     2401 cgaaaaggct acactgcttg catgttccag ctgctgcaag accggatcag gctgacgtgt
     2461 agcaaacgta tccgccggta aaaaaagtac ggggatatta tttgcgaatg ccgtacccgc
     2521 tgctgttaac agattggccg cgcccggtcc aacagatgtt gatacggcaa aaattttctt
     2581 gcgtaaattc tgtctggcaa aggcagtagc cgcatgggcc attccctgct cattttttcc
     2641 gctataactt ttcaaatgtc cgggctctaa ttccagcgcc tgaccgatcc ccaaaacatt
     2701 tccgtgacca tagacatgaa agataccttc aacataaggc gtttctttgc cgtcgatgct
     2761 gatgtattgt tgattcaaaa attttagtaa tgcttgtgct gttgttaaac aaaccgtctt
     2821 ttccattttt ttcaccttaa atccgagcga tcatttcgcc gtatatttct ttctccaatg
     2881 taataaaatc ttttacctcc tgagtcgtcg gtaaagaatc tgaacagtta ttgcttttga
     2941 ccatcattga tgcttccgct gagccaaact ctaaacaatc gatgatctcc cagccttcga
     3001 ataaaccgta taagaaaccc gagccgtaac catctccgcc gccaaagcct ttgcgtgctt
     3061 ccaccggaaa cggcttgatt gaaaactgct gctgatcggc cgtataagca gtcgatcctt
     3121 tcattccatg tttgattacg acaatctcag caccacaact gtgccaaaaa gcagcgcttt
     3181 cattatctga catgttaggc tggatcaact tctcagttag atcgaactct tctcttgaac
     3241 ccatgataat gtctgattcc ttagcaatca tcgaatagta aattgaaata tcatcgttgt
     3301 tcttccagtt ataagcacgg taatcaatat caaaaataat tttgacatta ttccttttcg
     3361 ccagcatcac tgccttgatt acggcttctc tggacgggct ctctgctagt gcagttcctg
     3421 agattaatag tgcttttgac cgtttcaagt agtcctcatc aatatcctca acgctaagct
     3481 gcaggtctgc ggcttgattt cgatacatca aaatattact ttcctttgat gagagcattt
     3541 cggtaaaagt cagccccagc ttttcaccat gctgcgcttt gctgatctga gacgtatcga
     3601 tgccttgttc attgaaataa tcgatcacat attcgccaaa ctgatcgtcc gagactgttc
     3661 cgataaaacc agctttcatg ccatgtttag taacacccac agcaatattg gcaggagatc
     3721 cgcccacaaa tttttcaaac atatggacct ttttcaatgg tttaaactct tctttgactt
     3781 gatcattgta ggcaggatta aaatcaatag cgactcttcc taaaagcagc aagtcaaatt
     3841 cttttgattg gtcaaacttt atataactca atatatccac ctctttatcc gttcttctat
     3901 ttctgaggca agtatagcac gccaaaacaa agcgtgcaat atttatttta gaacgcaata
     3961 ttgcgtgctg tttgaaaatg acggcaaaaa aaacgtgaat cgccttacat cacgattcac
     4021 gcctagatta aatattgtat tttgtcttaa taattatatc agtaaatagc gcatcttttg
     4081 taaccgtttt attattttgc agctgattcg ctaacatcag taatgaacga tagccttgaa
     4141 caaatccatc ttgatcgatt agaaaatcaa cctgatcgtt tttcagcatc tcgatattgt
     4201 tcttagtcaa atcataaatg attacaaagg gccgcttgct ttcatcgatt ttttccaacg
     4261 ctcgggcaat tcccgcttgt ccgccagaaa agacgactac gccatctaag tttgaataat
     4321 tctccaaggt gttgcttaaa atcttttcaa cctcagcact cgaatcaaag ctgctctgaa
     4381 cacccgtcag ctccagttca ggaaacgact ctttcaattc ttcaacaaag cctgccaccc
     4441 gtaagctgtt gacgctattt ccaaagtagc cggtaatcgc tagaaccttg ccctttcctt
     4501 ttgtcagcat acctaacaat cccgcagccg tttttccgct tttgaagttg tccatcccaa
     4561 caaaacactg tcgattcgta ccaagaatat ccgaattaaa ggtaatgatg aggatgcctt
     4621 gatcggtcaa ttgattaatt ttttctctga taccatcact atctactggc atgatcgcga
     4681 tcccttttac tccaagctcc accaatcgat ccaacgcctg cagctgggcc gcttcatcga
     4741 ccccgtctac ctcttctaaa aaacaatcaa tgtttcgctt ttttaaatcc ccgataatag
     4801 attccagacc tgatcggatc ggaatcataa aagaagattt cgccaattga gtgacaatcc
     4861 ccagcttgag cgttgtctct tttatgacct tctttttctt tggaacatag ttcatttcct
     4921 tggcgattgc tctgattctt tctgcaacct caggattgat ccttcctcga tcgttcaatg
     4981 cccgatccac tgtccccctt gacactcctg ctttctcagc tatttcttta attgtacccg
     5041 ccatcttgcc tctccttttt tcctattgtg tttattttat cacaaaactt aagaaaacca
     5101 ccggagaatg aattctccgg tggacttttt gtatgattaa ccttcttatt gaacatcact
     5161 gatcttaaac tctattccaa gggcgtttct ataatattct ggatacatct ccttcccaca
     5221 tttttcacag gtaaattggg gaggattgtt cggatcattt gcaatatctg catcatctaa
     5281 aaattttact acatccattg ggattgattc tttttcacca caacttagac atataaattt
     5341 cacgttggcg ttcccaatcc cagcgaattg ctttaaaagt tttttctttt tcttcttcat
     5401 actgttgcct gaattctttt tggtcaattt ttcgattctc ctctcgcata aaccgctttg
     5461 catggttatc tttcgcatat ttaataacta accgtccatt tttgcgcaca ctcatcccaa
     5521 cgaattcata gtactcaccg caacaagaac attctaaggg attttcacca aattcttcgg
     5581 ttattctttc ttcccaggat tttggtctta aggcctcatt cagattgata aacagctttt
     5641 ttactcgttt ctgatactcc ttcatgacct ttttcattaa tgttttgatt ctacgactat
     5701 agattccata gcgacgaatc gttttgaaat tcttattttg aatatggcga atcagtgcag
     5761 ctatgaattc gtctatactc atggtcatcg tctctttagt attcgttcgt ttgtccatgt
     5821 agttgaacat gacagtgtcg ccatcataca ttaagattcg gcttaatgcg atcgggccac
     5881 gcttcatata acgactgatg tattggagta gtgactttag atttgtcctg ctccttttag
     5941 gagcatttac gtaaaaccct tctccattgt tcttgtacgc agcttgtaat cttgattgaa
     6001 ctctttgctt gtcccactca cctaacgttc gtctaattag tttcaataca gcatttttcc
     6061 agtattttct taacatagaa aaaggtaaat aatcgtattc ttcccaactt ccatctcttg
     6121 ttaagccacc catagtaaca accatatgaa catgcggatt aaattctagt tttgatccaa
     6181 aggtgtggat tgtagctact atacctgcct tcacctttct tttctcaaaa taggcaagaa
     6241 taatttttgc ggcttcatcc attagtcctt tcagtaagga atctcgatag ttttccatca
     6301 taaagatttc ccgtaacccc tcatcgatcg taaagattac gtgccgatgt gtcactttaa
     6361 acatatcatt cgccacaacc gctgaccacc tttggctttc acctgttgca cacgttggac
     6421 agaactttcc acgacatttc aagggaacaa actttgtttg atgacatccc tcgcagacaa
     6481 acagtcggta tcctttttga atatctccac aatatctaaa cttgtttact tcccgtttga
     6541 cgactagccg aatctttcgc ccgtatttct caacaaagtt atcccaatta tggtgttcgt
     6601 caaaaaagat tgcttcgaga atattcgctt tcatagtaat attttacttg ttcaaaaaca
     6661 aaatgaaaag ggctaaaaga ctggtagtgc agccttttta gcccttaac
//
LOCUS       DAYYGO010000125         6644 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000125 DAYYGO010000000
VERSION     DAYYGO010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.128"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..859)
                     /locus_tag="ACHA5T_002422"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="ACHA5T_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="ACHA5T_002423"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="ACHA5T_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFM8887963.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="ACHA5T_002424"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="ACHA5T_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="ACHA5T_002425"
     CDS             complement(2143..2373)
                     /locus_tag="ACHA5T_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFM8887964.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="ACHA5T_002426"
     CDS             complement(2603..3421)
                     /locus_tag="ACHA5T_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8887965.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="ACHA5T_002427"
     CDS             3582..4271
                     /locus_tag="ACHA5T_002427"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFM8887966.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="ACHA5T_002428"
     CDS             4285..5787
                     /locus_tag="ACHA5T_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8887967.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="ACHA5T_002429"
     CDS             5800..6276
                     /locus_tag="ACHA5T_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFM8887968.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="ACHA5T_002430"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="ACHA5T_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2174 a         1032 c         1203 g         2235 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttattatcca aaaataataa aaaattggag cgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt catgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atgt
//
LOCUS       DAYYGO010000126         6313 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000126 DAYYGO010000000
VERSION     DAYYGO010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.129"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(99..1229)
                     /locus_tag="ACHA5T_002431"
     CDS             complement(99..1229)
                     /locus_tag="ACHA5T_002431"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFM8887969.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="ACHA5T_002432"
     CDS             complement(1878..3080)
                     /locus_tag="ACHA5T_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8887970.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="ACHA5T_002433"
     CDS             complement(3140..3343)
                     /locus_tag="ACHA5T_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM8887971.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4549)
                     /locus_tag="ACHA5T_002434"
     CDS             complement(3485..4549)
                     /locus_tag="ACHA5T_002434"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM8887972.1"
                     /translation="MDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERFE
                     ELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQF
                     FRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINNE
                     SNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAKI
                     ISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQSF
                     EKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYYQ
                     EEVEYYERKLQSISNEKTSY"
     gene            complement(4610..4717)
                     /locus_tag="ACHA5T_002435"
     CDS             complement(4610..4717)
                     /locus_tag="ACHA5T_002435"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8887973.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4880..5692
                     /locus_tag="ACHA5T_002436"
     CDS             4880..5692
                     /locus_tag="ACHA5T_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8887974.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6066..6257)
                     /locus_tag="ACHA5T_002437"
     CDS             complement(6066..6257)
                     /locus_tag="ACHA5T_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887975.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
BASE COUNT         1875 a         1298 c          996 g         2144 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttc
//
LOCUS       DAYYGO010000127         6304 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000127 DAYYGO010000000
VERSION     DAYYGO010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.130"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..506)
                     /locus_tag="ACHA5T_002438"
     CDS             complement(<1..506)
                     /locus_tag="ACHA5T_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFM8887976.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSM"
     gene            817..1809
                     /locus_tag="ACHA5T_002439"
     CDS             817..1809
                     /locus_tag="ACHA5T_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8887977.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            1869..3290
                     /locus_tag="ACHA5T_002440"
     CDS             1869..3290
                     /locus_tag="ACHA5T_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFM8887978.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            3283..4782
                     /locus_tag="ACHA5T_002441"
     CDS             3283..4782
                     /locus_tag="ACHA5T_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFM8887979.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            4821..6215
                     /gene="uxaC"
                     /locus_tag="ACHA5T_002442"
     CDS             4821..6215
                     /gene="uxaC"
                     /locus_tag="ACHA5T_002442"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFM8887980.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         2041 a         1180 c         1285 g         1798 t
ORIGIN      
        1 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg
       61 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      121 ttagcgatta gtttacgtga ttgattttcg tacagttcca tgagaatcgc ctgcttcagt
      181 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtccgtcca
      241 caatcatggc agagaaaacg ggtacgtttt aattctaaaa tagtcaactt ttctttgact
      301 ttcaacatct gagtgcgtgt cgtataggaa ccgttgcgga taatacgaat cgatccacag
      361 tgttcacaat gggaaggtga gtcatccaat ctccctttaa taaactgcgc ttgaacgcct
      421 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg
      481 agtaattttc tagtatgatt atccattgag agactccttt ggtagggtga cgttttggtc
      541 gactaaattt taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa aagtgttagt
      601 agaaaatcgc tttcgatttt ccaccaacac aaaatattat agaaccctaa ttaaatgagc
      661 attccccgct ttcaagaata gcataattaa aaataaaaat ccaagtaatt tttaacgtgt
      721 gcaatctttt catatcaaag atctattgac ttatcgtagt tatcgtaata tgatgaatgc
      781 gttaacaaaa agtgaaaaag taaggaaaat gatggaatgg taacgatcaa agatatcgct
      841 aaagcagctg gcgtttctca tacgacagtt tctcgtgctt tgaataacag cagtttaata
      901 aaaaatgaaa caaaacaaaa aatacgacaa ctagctaagg aaatgaatta ttcgccaaac
      961 tatagtgcaa aaggtttagt gaatcaaaaa aaatatctaa tcggactatt tttctcaagt
     1021 ttgcataaag gaacttcatc aaatttccta gcagattcca ttgcaggcat ccattcagta
     1081 ttagacacaa attacagttt atctgttgaa agtatcgatg ttgttgattt aacagaaatc
     1141 aacctgcaac gctacgatgg actgatcgtt atgagtcaaa gtgatgatga tcagcctttc
     1201 atcgactatc tcaaagaaaa agaatttcct ttcgttgttg tcaatcgtca catcaatgat
     1261 ccagcggtta tcaacgtcac tgccgatgat gcggccggtg ttatgcaggc agtagattac
     1321 ggtattggat gcggacatac gaagatcgct tatattggcg gaaagaaaaa ttttcgttcg
     1381 acaaaagaac gggaaaaagg ggtattgaaa agtctaaaaa aagcgagcat ccctgtcaat
     1441 ctagcctatt ttttaacagg agattacagc ttagaaagcg gcctccaaaa catggaatac
     1501 ctattatcac tttctgacct gcctactctg gttttttgcg gcaacgacga catagcgatt
     1561 ggtgcattga gagctgtaca tttagctggc ctccgtgttc cccagaacat ttctctgatc
     1621 gggttcgatg attctccagt cgtctcctat ctcaatccgc cgcttaccac cgtacgtaag
     1681 cctttgaaag aaatgtgtca agaaggaaca aagcttttac tagatttgat cgaaggcagg
     1741 acgatcgaac aatctaaaat tgagctctcc accttgttga tccttcgtga gtcgatcaat
     1801 tacttgtaaa ctacaagtaa ttttatataa aaatgttaac gtgtgcataa cacagaaagg
     1861 aagtttatat gaaaaaactg acaaaagaat tagtgaaaaa aaacgaagca cctgtaaaag
     1921 ttcttcagtt tggtgaaggc aattttatgc gtggatttat tgattggcag attcagcagc
     1981 tgaataacca acaattgttt aaaggtaatg ttgcaatcat tcaaccttta gaacaaggtc
     2041 ttggaaatat gatgaaccag caagataatt tatatacggt cattttacaa ggactcagca
     2101 atggcgaaat cgttgatact tccgagatta tcaccgtagt agaacaaatg atcaatcctt
     2161 atgatgaatg ggatgcttat ctatctctag cagaaaatga cgacttggcc tatatcgtct
     2221 ccaatacaac agaggcagga atccaattcg tggagactga taatttaaca gatactcccc
     2281 cacagagctt cccgggcaaa ctgacagcct ttttataccg acgtttcaaa ttaaacaaag
     2341 ctggctttac catcattcct tgcgaactga ttgacagaaa cggcgaacgt ctaaaagaaa
     2401 tcattttacg ctacgcagaa aaatgggatt tagaacctgc ttttgttgag tggttagaaa
     2461 aagaaaatgt tttctgttgt agtttagttg atcgcatcgt cccaggttat cctcgcgaag
     2521 atgcaggtgt tttcaatcaa cggcataact atgaagatca attgatggtc aaagcagaac
     2581 cattcatgct ttgggtgatc gaagggccta aagaattgga gaatcaacta ccattcaaac
     2641 aggctggctt gaatgtcatc atcacagatg atatgactcc ttatcgtgaa cgtaaggtgc
     2701 atttgcttaa tggaccgcat acagcgatgg tccctcttgc tttgttggcg gaattggaaa
     2761 ctgtagaaga agtaatgaaa gatcctttat tctccgcata tgttgatcag ttattcaatc
     2821 tagaattgat tcctatgctt tctttaccta aagatgaatt agccatttat gctgaccaaa
     2881 tcaaagaacg tttcttgaat ccttttgccc atcacaaatt agaagcgatt tcattaaata
     2941 gtgtttcaaa gttcagcacc cgtctgcttc cagtgttcaa gaagtatatc gaagaacaaa
     3001 atcaggtacc accgttaatc actgtttcac tagctgcgct acttcttatg tatcgaggcg
     3061 atcaagtaaa gccacatgat gatgaaaaga cgataagcga atttactgat gcttggtctg
     3121 ataatggaac agctattcct cgattattgc aaaacgctgc gctttgggga gaagatttgt
     3181 ctcaaatacc gaatgtgaca gatacagttc aagaaatagc tgcaaagatc gatcaaaaag
     3241 gtgtccggtc agtattacaa gaaatgaaag gaacgatggt ccatgcttga aaccctgcaa
     3301 ccaacaatga aattaaatcg acgagacagt gtggtcgtcg cattaacccc tattccaaaa
     3361 caaacacaac tgacgattga tcagcaaacg atcaccactc ttgaagatat cccgcaaggt
     3421 cataagattg cattgggtga tttgaaagct ggagacaacg tgatcaaata tggctatcca
     3481 atcggtcatg tgacgacaga cgtaactgcc ggtcaatggc tgcacactca taatgtaaaa
     3541 accaatcttt ctggtgaatt ggattatcga tatgaaaaag atgtccatcc aagtcattac
     3601 ctttttgaga accgaacatt tcaaggttac ttgcgaaaaa atggaaaagt agggattcgt
     3661 aatgatttgt ttttagtacc taccgttggg tgtgtgaatg gcattgctga actgatcgtc
     3721 aaacagttca aggaaaaaca tccagatctg ggacagtttg accatattac gattttaaag
     3781 catccttatg gctgttcgca gcttggaaaa gatcatcaaa atactcgaga aattttagcg
     3841 gacgcagtaa atcatcccaa tgccggagga gttttagtat ttggattagg ttgtgagaac
     3901 aatacagttc ctgaattcaa aaaaatttta ggtgaatatg atgaagaacg agtgaaattt
     3961 ctagtggctc aagatgttta cgatgagatc gaacaaggtg tggctctatt agaagagctt
     4021 ttaacagcgg cggaaaatga ccagcgcata tcagtcccgc tctctaagtt gaatgtcggt
     4081 ttgaaatgcg gcggttctga tggtctatcg ggaataactg ctaatccttt acttggtgca
     4141 ttttctgact acttgattgc tcaaggaggc agcacgatct taacagaagt gccagaaatg
     4201 tttggcgcag aacaagtatt gatggcacga gcggaaaatg aagaagtttt tgactcaatc
     4261 gttgacttga tcaatgactt caaacagtat ttcttgtctt acggggaacc agtttacgaa
     4321 aacccatctc ctggtaataa agcaggtggg atcactacat tagaagataa gtctttaggc
     4381 tgcacgcaaa aatcaggaag ctctcctgtc gttgatgtgc tacaatatgg cgaaaaaatc
     4441 agaaaaccag ggcttagttt attgcaagct cccgggaatg atcttgtagc tgcctctgct
     4501 cttgcttcat ccgattgcca gttggtcttg tttaccacag gaagagggac tccgtttggc
     4561 tcatatgtcc caacggtcaa ggtatctacg aatacaactc tttttgaacg caaaggtcat
     4621 tggatggatt ttaatgcagg cgtcttatta gaacaaccaa tggaagtaat cttagaatcg
     4681 tttgttcaaa aaatcatcgc tgtagctagt ggagaagaaa cgaaaaatga acaaaatgac
     4741 gttcgagaaa ttgccatttt taaaaacgga gtaacacttt gattttcata aaacagtata
     4801 aaaagaaaga aggaagattt atgtttttat ctgatgattt tttattaaaa gacgattggg
     4861 cgaaaaagct ttaccattcc tacgcaaaaa acatgcccat tattgattat cactgtcact
     4921 tagatccaaa agaaatttac gaaaacaaca atttcagcaa tctgacagaa gcttggttag
     4981 gaggcgatca ttacaaatgg cgcttgatgc gagcttgcgg cgtgcctgaa gaaaaaatca
     5041 caggaaatgc ttctgatttt gaaaaatttt tagcttggtg tcaaaccgta ccaaaaatta
     5101 tcggaaatcc gctttacagt tggtcacatc ttgaattgaa gcgtttcttc ggtattgact
     5161 tgttgataaa tgaagaaaat gcagagaaaa tttggacaca agcaaatgaa attttggcag
     5221 ctcctgattt tcgccgtcgg gaaatcgcaa aaaaatcgaa tgtagaagtg atttgtacaa
     5281 cagatgatcc cgcagacgac ttgcactatc atcagttgct tgctcatgaa gaaccagatt
     5341 tgaaggttct accttcattc cgtccagata aagcactcaa tattgaaaaa gaaggattta
     5401 atgcatgggt cacactactg gaagaagcag ctgaccaaac gatcaccacc tatcaagaac
     5461 tgatagaagt cttagggcag cggatagaat acttccatca acatggatgt cgtgtttccg
     5521 accacgcttt agacatctta cgctataaag aagcggatga aacagtatta gaagagattt
     5581 ttcaaaaagc acgagctaac aaactattat cttcagatga aatcgatgct tatcttacag
     5641 aaacactgat acatttgacc cacttctatc attctcataa ttggacgatg cagctgcaca
     5701 tccatgctta tcgtaactgt aatacacaaa tgttggaaaa attagggccc gataccggct
     5761 atgatggaat gaatgatctt tctttgacta ttcctctgca aaaattattg aaccgtgcag
     5821 aagaaacgga tcagttgcca aagacgatcc tttattcatt gaatccgaat gactatcctg
     5881 cgattttagc tttgatgggc tgtttccaaa aagaacagaa tggaaaactg cagttaggtt
     5941 ctggatggtg gtacaacgat actcgagctg gtatgcgcga tcaattgaca caatttgctg
     6001 atggtagtgc tttggggaat tttgtcggca tgctgacaga ttcaagaagt ttcctgtcct
     6061 acactagaca tgaatatttc cgtcgtgttc tttgtgaatt tttaggcgaa atggtcatgc
     6121 gaggagaagc acctgaagat gaacaacttt taggaagtct agttcaagcg attagttata
     6181 caaatgctaa aaattacttt ggcttttttg cctaattgat taatagtaac agtaacgaat
     6241 caaaaacagg atagtgaaat aacggaatcc gtttatccac gttttagaga agaagacgga
     6301 ccaa
//
LOCUS       DAYYGO010000128         6303 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000128 DAYYGO010000000
VERSION     DAYYGO010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.131"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            334..714
                     /locus_tag="ACHA5T_002443"
     CDS             334..714
                     /locus_tag="ACHA5T_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFM8887981.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPD
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            696..1610
                     /locus_tag="ACHA5T_002444"
     CDS             696..1610
                     /locus_tag="ACHA5T_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM8887982.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKK
                     KVRSNKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTP
                     EQSEIRPTRRNHEQHVERGTDHQQELSL"
     gene            1855..2544
                     /locus_tag="ACHA5T_002445"
     CDS             1855..2544
                     /locus_tag="ACHA5T_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887983.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLV
                     GVIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTA
                     CYEDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQT
                     EEACCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLES
                     VKEWLDQQQQN"
     gene            complement(2665..2751)
                     /locus_tag="ACHA5T_002446"
     CDS             complement(2665..2751)
                     /locus_tag="ACHA5T_002446"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFM8887984.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
     gene            3427..4377
                     /locus_tag="ACHA5T_002447"
     CDS             3427..4377
                     /locus_tag="ACHA5T_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFM8887985.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
     gene            4398..4931
                     /locus_tag="ACHA5T_002448"
     CDS             4398..4931
                     /locus_tag="ACHA5T_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM8887986.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            4947..5117
                     /locus_tag="ACHA5T_002449"
     CDS             4947..5117
                     /locus_tag="ACHA5T_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887987.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            5567..6100
                     /locus_tag="ACHA5T_002450"
     CDS             5567..6100
                     /locus_tag="ACHA5T_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887988.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
     gene            6112..>6303
                     /locus_tag="ACHA5T_002451"
     CDS             6112..>6303
                     /locus_tag="ACHA5T_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887989.1"
                     /translation="MFLISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQ
                     LYKSGLFLVLILVVLDIIKS"
BASE COUNT         2324 a         1103 c         1173 g         1703 t
ORIGIN      
        1 aaaaaaaaat aaatcgatac attttagaag aatagggttg tttgaatatt acaaagcaac
       61 aatttcacct tcggtgaaat aagcgaaact ttacaaaaag tggctagcaa atgctagcct
      121 ttttttgtat tgtggagcgt ggattttgag ggggattcaa gggggagctt gcgctccttt
      181 gacaagcggt tatttgcacc aaatacggag gtatttggct agcaaatata gagcttgcca
      241 aaccctttta gtgagcttta ctcattgtac ctttttaggt acaatggtat tgcgaacgat
      301 acgtccgtag gacgtactga aagggtggga cttatgagcg aacaacatca aaatttggct
      361 agcgatcatt ctaaaaaata tacctatcga tccgagccaa aacaaatcag ttttcgagtg
      421 agcgaatccg aatttgcaaa gctgaagcag tcagctgaag ctttgcaaat gagtgtgccg
      481 gctttcgtga aagccaaggc acaaaacgcg cgtctggtga caccgaaagt ggcgccagac
      541 attgcccagg caatcgcgcg cgatttagcc aaagctggag ggaatatcaa tcaaattgcc
      601 aagtggtgca atacacacca acacgacgtt gctcctgatg atgctcaacg tttatccgaa
      661 aacctaaaaa tcatgcaaaa ggagctacaa aagatatggc aacagttaaa gtaagtcgaa
      721 ccacttcatg tagccgagca atcaattacg cagaaccacg agcaaccgtc aaaacaggaa
      781 taaactgtga cattgattat gccaaaagtg aaatgaaaca aatacgcacg ctttatggaa
      841 aagacgatcg tgtacaagca cacttattga ttcagtcctt tcgaccagga gaaattaccg
      901 ctgaaaaagc caatcaacta ggggcagagt atgcgaaaaa aatagcccct aaccaccaaa
      961 tcgcgattta cacacataca gacaaagacc atattcataa tcacattgtc attaactccg
     1021 ttaatcttga aactgggaaa aaatttcaag cccatgggca agcattttta aacaaatgtt
     1081 atgacctaaa tgacgagatt tgtcttgaac atgacttaac aatcacggaa cgaggaaaga
     1141 aaaccgaaaa acgaaccatg tctgaaatta aattaaaaga aaaaaatgaa ccggtttgga
     1201 aagacgaaat tcggtttgcg attgatcaaa caatgaaaaa caaaaaaact cgaacgtata
     1261 cccaattttg tgattcactt aaaacattcg gcattcattg cttgaatcgc ggaaaaaatt
     1321 ttacttacga attggtccac aaaaagaaaa aagtacgatc caacaaacta ggaaaggatt
     1381 acgaaaagga gacgatttta catgagctgg acagacgaga acgaacctac aatcaacaac
     1441 gaaccaacga gctacgaaga gctcgagcta gcattcgcga aactgcagaa gaagtacgaa
     1501 gagaacaacg aacttttacg cctgaacaat cggaaattag accgactcga agaaaccatg
     1561 aacaacacgt tgaacgagga acagaccacc aacaagaatt atcgctctaa aacgttaaaa
     1621 gacatcgaaa acgaaataaa gagacaacgt gcctttcttg cgccttatac ccaagaaagg
     1681 atacaaacta cgatggattc acaagtcaaa aaactccacc aagcgctatt agaccagcga
     1741 aaccaagaac aaaaaaaggc tttaattcaa cgatcgatgg atattcttgc ccccttatgt
     1801 tgcttagtac taacattatt agccttttgg ttggtatggg gagaataaac agacatggca
     1861 ttctatttac cctatctact catttctgcc agtattttag gaattatttg gcttagctat
     1921 aaggcttatc aaacaagata ttcattaaaa ggctctaaaa ttcgatttaa gcgattttta
     1981 ttggtcgggg ttatatttgc cttatcaatc gttttaagtg gtttattcgg cgttttagag
     2041 ggtaataaac gtgtttcaag aagcatttta ttaggggata tcactcaaaa gtatgaagca
     2101 gcatgtgata aaaagaaaaa agagcaagca ttagcacaaa aaatggagga atttaccgct
     2161 tgttatgagg acatgaacaa tatttttgtg gatcaagaga aacgtttaac agataaaaac
     2221 atgaaaaaac tcactcgttt ttatcaaaac ttaccagaaa aacagcaaaa agaggtacaa
     2281 gaaaaatatg agcaagtaaa aaaagacgtg caatatttta aagatacgca gacagaagaa
     2341 gcttgttgta acttatttag cgatacaatc cctttttcaa ccagtgaaca agaacgaaaa
     2401 gaacggcaac aatcggtcac atacgaacgt tatgaaacac tgcttcagca agcaacaaac
     2461 atacaaaatc caacgaaaaa agaaacggca ttaaattatc tagaaagtgt caaggaatgg
     2521 ttggatcagc aacaacaaaa ttgaatttga ttagcaatca tgttaaactg aacctgcaaa
     2581 tgatcaaaca ttagatacac gaaagcccca gagaggtcgg attctctggg gctttttttc
     2641 aggttgatgt cgcctttatt gattttactt cttgtttcgg ttattcaacc aataactgaa
     2701 taaagagaca caaacttcta caagaagggg gcaaatgaac cgttcaaaca ttagatacac
     2761 ctccttccag tcgcaagact gtcggatagg cgacaagtta ttatacctta caaaataaaa
     2821 tctgtgtggt gtttttaagt atcccttatt ttcgggttgt tagggcttgc cctgaccgcc
     2881 tgtaaggcgc tttaagaaca agataaggtt ttttacccaa gactgtctta aaacgactta
     2941 gacgagctat cagaggcttc ccccacctcc gttactgact tgtttttagc aaaggagcct
     3001 ttaggcgact gcgcagtaca atgtgagagc atagcgatca cactttttta gcgaccgtag
     3061 ggagcaaggt tttgaattgg cagttttgaa aaatgcgcga acctgttggt agaaaaaaga
     3121 agtggttttt aggcgggaaa ttttgtcacc ctcttttata atctcttttt aaaccttttt
     3181 ttaaacctct ttaccgtcgg ctgtgtcctt actccttcaa tggatacagc gttttataaa
     3241 ctacattttg tctgctttta aactacattt tgtctgcttt taaactacat tttgtctgct
     3301 tttaaactac attttgtctg cttttaaact ctttttttgc actcacgtat gttataatat
     3361 gcattatcac agacaaaacg ttgtttataa cgtctgtatc cattgaagga gtaaggagga
     3421 atatctttgg caaatgaaat cgtgaaatac catcatgaat taaataccat tccgttgcgg
     3481 aaatttacac cagttgaaat gaatctattt ttctctattg tgtctagaat gcgcgaaaaa
     3541 ggcgataaaa cagtccgtct tacttttgac caactaaaag atttaagtaa ttataaagca
     3601 actgctaata atcgtttcat taatgattta gaaatgactt atgataaatt aatggattta
     3661 cgttttggta aaagaagtgc taatgggctc gatatagata gattcgttat gtttagtgag
     3721 tttaaaatac gtggaactgc taaagatcca tatgtagaca tacaagttta cgaaaaagct
     3781 ttacctttac taaacaattt agatgagtgg gttcgctatt cactccaaca atttaacgaa
     3841 ttacaaagta gctatgcaaa gactatgttt cgattactaa aacaatatcg aactaaaggt
     3901 tttgcttatt tttctaaaga ggattttcat gaactattag atattccaaa aagttactgg
     3961 aacaaccaag caaacgttga taatcgtgtt ataaagccta tacgacagga attaaccgca
     4021 atcttcaaag gattatcaat caaaaagaaa tacggaaaag ggcgaggaaa acctgtcgtt
     4081 ggctatcaat ttacgtttaa accagaaatc aaaaaagcag atgattttaa taaacatgca
     4141 ccaaccaaag aagaacaaaa taaagcattg gctttagctg ataaacaaaa agaatacgaa
     4201 gaacctgcta caacaattaa cgaaaaaatc aaacagaaga aacgagaact tgaactcctc
     4261 caacaaatca aacaactaga aaaagagaaa gaagctcttg aaaaacaaac agagtccgca
     4321 ctttcgaaaa aagagaaaaa aagtttatta tctgatttag ataatttatt ccagtagcaa
     4381 cctaggaggc aaccagtatg caaccacaag aaacctttac aataaaggaa ttgtcagaac
     4441 tttttaagat gagtaggcaa gcaatatcga aacatatcca aaaattagat tcttccatga
     4501 tagctaaaaa tgagcgtggt tataaggttg tattgcgttc tggagtattg caacttgctc
     4561 gcaacttagg caaccaaatg ttgctagata ggttgcacga gtacgaacaa gacaacgata
     4621 aaaatgttaa tataccaagc aaaaacgtgg ttacaaacaa gttgctagat cagttgcgtg
     4681 aagaaaatga atttttacga gaacaacttg ccctcagaga gcaaaataat tctcatatac
     4741 aaaaactact cgaccagcaa caacaactca ccctacaagc caacaaacaa atcgaaaaac
     4801 tgcaagaaaa actacagttg acttatacag aagaagctcc agagaatgac tcaacgactc
     4861 tttctgaagc aacagaaaca atagacaaac aaattgatac tgaaaaaaaa tggtggcaac
     4921 tttggaaata actggaggga acattcatga caagaaaaaa tcttctattc ggttctattt
     4981 tactaggaat tctaggattc agtccgatgt tattgtcagt gatcaataaa gggaataaac
     5041 atggtgaatt gaaattgaat ccaaattatt acaacagtcc taacgaagaa ttgtttgtaa
     5101 aagaaacaga taaataataa aaaagactgc tctttcgagc agccagttaa aaaataatga
     5161 gtgtttattc ttgaaaaagc aacgcgctta tgaagcgcta ccaaaaattt atcatatttt
     5221 attaaaaata acaacccagt aaaaaatgac tggtaaaaat tcacaaagaa acggaaagaa
     5281 gcaaccgcag aaaatcaagc ttaaaaagag cttttgacct agaccaatcg aaaatcacaa
     5341 agtgagcgcc tgtatctatt gctacaggcg ctacttggca tagtacacat tatgtcaaca
     5401 agaatttaat ataacatatt ttcctaatgc attctaacta taccacatta tcaaaaatat
     5461 aaattatctt tactaaataa aatattgaca agcctatacc ttttgtttta tgattttcac
     5521 aaagaaaacg ttttctatat atttttctgt atcaggagga aattctatga aaaaatttac
     5581 tttaggggta gctttgttaa ctattggggc agttgtttta ccgtcaacta ctatagttta
     5641 tgcttcagaa aattcccaag tggtatcatt aaacgaaaac aatgctactg aaaatgtagt
     5701 tattgagtac actgaagatg gtatggtcgt aactaaatat gaggatggtt atagtgcgga
     5761 agattttgca aattcttcag atatcatgac aagagctgca gttgcaagat ggggcaaaaa
     5821 ggaatataca aatattgctg taaccactgg agtagctgct aatgctatta atgctgcctt
     5881 ttgggcagga gggaaagcta gcacttctat gtttggtatc ccaggttggg caattggtgc
     5941 tttgttaaca ggtgcaagtt ggactaaaaa aggtgcttct ccagggaatg cagttgcaaa
     6001 agaatgggac aaaaatggta atggctggat tggattttat taccaaaggg gatatgatgc
     6061 agctggaaga gttgtagcta ctagatattc aacgttataa gttaaggagt gatgtttttg
     6121 atatctaaat cattaaaatc attaaaaaca tttactaaaa agaatccgca aatagctact
     6181 attcgtattt ttatatttgc tatagctgtt gctataattg ttaatatgct aagcggaggg
     6241 caattataca aatctggttt atttttagtg ttaatcttag ttgtcttaga cataataaag
     6301 tct
//
LOCUS       DAYYGO010000129         6221 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000129 DAYYGO010000000
VERSION     DAYYGO010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.132"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(41..229)
                     /gene="rpmB"
                     /locus_tag="ACHA5T_002452"
     CDS             complement(41..229)
                     /gene="rpmB"
                     /locus_tag="ACHA5T_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFM8887990.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            499..861
                     /locus_tag="ACHA5T_002453"
     CDS             499..861
                     /locus_tag="ACHA5T_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8887991.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            913..2595
                     /locus_tag="ACHA5T_002454"
     CDS             913..2595
                     /locus_tag="ACHA5T_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFM8887992.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2712..4748
                     /gene="recG"
                     /locus_tag="ACHA5T_002455"
     CDS             2712..4748
                     /gene="recG"
                     /locus_tag="ACHA5T_002455"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFM8887993.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4797..5798
                     /gene="plsX"
                     /locus_tag="ACHA5T_002456"
     CDS             4797..5798
                     /gene="plsX"
                     /locus_tag="ACHA5T_002456"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFM8887994.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            5920..6162
                     /gene="acpP"
                     /locus_tag="ACHA5T_002457"
     CDS             5920..6162
                     /gene="acpP"
                     /locus_tag="ACHA5T_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFM8887995.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT         2067 a         1085 c         1423 g         1646 t
ORIGIN      
        1 aaaaaccgtt cactatctga acggtccaag tatcattcaa ttaaacgcgt tcaacttttc
       61 ctgatttcaa agcacgagtt gacacccaaa cttttttagg ttttccgtcg accataacgc
      121 gaactttttg taagttaggt ttaacagtac gttttgttga gttcatagcg tgtgaacggt
      181 tgtttccact ttttgtcttg cgacctgtaa agtaacaaac ttttgccatg gtaatttcct
      241 cctttgcctt gatttggagc aatagaataa ttcagctcat accagactaa catatcatat
      301 agaatggaaa tttgcaagta cattttcata gatacaattg acaattgaga agaataattg
      361 taaattaaac aaggttatac ctgaaatttt gcaaggtttt tgagtacttt gggtaactgc
      421 cttttatttc aagttgcgct atgataaaat atgatagaag aaaaatggga gttttttacc
      481 cgttaaggag gctcattcat ggctgtaaaa attcaaacgt cagctggaac cattgaaatt
      541 accaatgatg taattgcaac agttgttggt ggcgccgcga cagatgtgtt cggtgttgta
      601 ggaatggcca gcaaaaatca aattaaagat aatattactg agattttgcg taaagaaaat
      661 tactcaagag gagtagttgt ccgccaagaa gataatggta ttgctgtaga tgtttatacg
      721 attatcagct atggtacgaa aatctctgaa gtttcccgca acgtccaaga aaaagtgaaa
      781 tacaatctag aggcattatt aggcgtaact gccaattcag ttaatgtctt tgtacaaggt
      841 gttcgtgtac tgccagacta ggcaggagac tggctggtaa ctcagcagtc gatctgaagg
      901 aggattatct aggtgagtgt aacagaaata agtgcaagcc agttccagga aatggtccag
      961 gcaggtgcga atcgtctgca agtaaatgca gaatacgtca attcattaaa tgtattccct
     1021 gtgccagatg gcgatacggg aacaaatatg aatctatcta tgactagtgg agctaaggca
     1081 gtagtagatt caacctcaga aaaagtagga gaattagcag caatgctttc aaaaggattg
     1141 ctgatgggtg cgcgcggaaa ttccggcgtt attttgtctc aattgtttcg tggattttct
     1201 aagcaaattc cagatgttgt ctcattaaat gcttccgatc ttgctgcagc cttcactcat
     1261 ggagttgaaa ctgcgtataa agcagtaatg aaaccagtag aaggaacgat tttgacagta
     1321 gctagagaag cagcgaaagc tggagaaaag aaagcaaaaa gtactgatga tgtcgttgaa
     1381 gtaatgacag ctgttgtaaa aggggcaaag cgtgctttag ctaaaactcc agacttgctt
     1441 ccagtcctaa aagaagtcgg tgtcgtagac agtggagggc aaggactgct gtttgtttat
     1501 gagggctttt taagtgcatt gaatggagaa ttccaagcgg atgaaacata tgaaccttct
     1561 ccagcagaaa tggatgatat ggtcaatgca gaacatcatc gcagtgtcca aggtcaaatg
     1621 gcgacagaag atatccattt tggttactgt acagaaatca tggtaaaaat cggagaagga
     1681 cctacagtcg atagtacatt cgattacgaa gaattccgta actatttaga tggtatagga
     1741 gattctttac ttgtggtcaa tgatgacgag atcatcaaag tccacgtgca tacagaaaac
     1801 ccaggagaag tcttgaatta tggacaaaaa ttcggttcat tagtcaaaat caaagtcgac
     1861 aacatgcgct tgcagcatga gacgattttg gaacacgatg aagaagtgaa cgagtttgcg
     1921 aaaaaagaaa caccacgtat cccgtatggt atcgtagcga tcgctgctgg taaaggcgtc
     1981 caagagctgt ttgaaagttt aggcgctaat tatgttatca gtggtgggca aacaatgaat
     2041 ccaagtacgg aagacattgt taaagcgatt caagaagtaa acgcagataa agtgattgtc
     2101 ttgccaaaca ataagaacat cttcatggca gctgatcaag cagcagaagt agcagatatc
     2161 ccagtagcag ttgttccttc aagaacgatt tcccaaggat tgacagctat gctttcattc
     2221 aatgatcaag cttctttaga agaaaacaaa gaaacgatga cagaaatgtt gtctagcgtt
     2281 gtgagcggtc aagtcactca cgcgatcaga gatacctcaa ttgacggtgt agagatcaaa
     2341 gaaggagact ttttgggcat gatcgatgga aaaatcctga tctctaaccc agatatcaca
     2401 gcaacagcgt taagtacact agaacaaatg atcaatgagg acacagagat cgtaacgatc
     2461 ttggcaggtg atgaaggaac agttgaacaa gcaaatgaat ttgctgagaa actaacagaa
     2521 aaacatcctg atttggaaat cgagatccac caaggagatc agcctgtcta tccttatcta
     2581 ttttctgctg aataatttct gtgtcagaat atctttctaa cacagtattc attcataaaa
     2641 ggccttcggt tagtgatcag gtatgatcgc tgaccaagtc ctttttttgt taagggaggg
     2701 aggaaaaatt tatgcgttct ttagatgatc caatcactat gctggcagga gtcggagaaa
     2761 aaagagcagc tagtcttgcc agcttgggca tccaaacgat tgaagactta ctgacttatt
     2821 atcctttccg ctacgaagat attcaggaaa gaaatctaaa cgagatccaa gatcaagaaa
     2881 aggttacttt gaaaggaatc gtagtctcgc ctcctgtgat gaatcgattt ggttataaaa
     2941 aaagccgatt gcaatttcgt atgatgcaag aacatgatgt gttcaacgtt tcttttttta
     3001 atcaacctta tctaaaagaa aaagtcattt tatcggagga aatcgccgta tacggtaaat
     3061 gggatgcaaa gcgtaaagca ttgaacggga tgaagatttt aggttctcaa acaatggatg
     3121 acttttcgcc gatatatcac gtcaacaaat caattcgtca aacgacatta gttgatctaa
     3181 tcaaaaaagg gtttcaagaa tacggcacat tgattgaaga aaatctgcca aacaaccttg
     3241 tggagaaata tcgactgctt gaccgagctt ctgctgtaag aagtatgcat tttccaaaag
     3301 accacgaaga aaaccatcaa gccaaacgac gtgtcgtttt tgaagagttc tttttatttc
     3361 aaatgcgtct tcaaggattg aaaaaagcag aaaaagcaga aacgaatggt ttggagatcc
     3421 tttatgatgt ccaaaaattg aaagaattta ctcaaaagct accttttgaa ctgacaaatg
     3481 cacaaaaacg tgtaacgaat gaaatctgtc gggatcttcg cagtccgcag catatgcagc
     3541 gacttttgca gggagatgta ggtagcggaa aaacagtggt tgccgcgatt gcgttatacg
     3601 ctaccgttac agcaggtttc caaggtgcgt tgatggtgcc tacagaaatc ttggcacaac
     3661 aacatatgga aagcttgcaa cagctgtttg atcccaatga agtaaccatc gcattgctta
     3721 cggggtcaac gaaagcaaaa gaacgtcgtg agctccttga aaaactagag caaggggaaa
     3781 ttaacatcgt gatcggcaca catgcattga tccaagatgg agtcgaattt cagaatttag
     3841 gtctagttat tacggatgag cagcaccgtt ttggcgtgaa ccagcggaaa gtacttcgtg
     3901 aaaaaggctg gcgaccagat gtcttattta tgacagcgac tccgattcct agaacattag
     3961 ctattacagc atatggtgag atggatgtat ctatcattga tgaacttcca gctggacgta
     4021 tcccgatcga aacacgctgg gtaagaacac ctcaattgga cagtgtactg gattggacga
     4081 aaaaagaact ggcaagagga catcaaatgt atgtcatctg tccgctgatt gaagaatcag
     4141 aagcattaga tgtaaaaaat gcagtggaaa tctatgaaaa acttcgtgaa ctatttgcac
     4201 caaaatatga agttggcttg ttacatggta aaatgaagaa tcaggaaaaa gaagtaatca
     4261 tggaagcctt caaagacaac cagatgcaga tattagtttc gactactgta attgaagtag
     4321 gggtaaacgt gccgaacgcg accgtcatgc tgatcatgga tgcggatcga tttggattgg
     4381 cacagctgca tcaacttcgt ggacgtgttg gacgcggaag tgatgcttcc tactgtatct
     4441 tagttgctaa tcctaaaaat gaactaggtg tggaacggat gaaaatcatg acagagacca
     4501 ccaatggttt tgtactaagc gaaaaagatc tggaattacg tggtcctggt gaagtatttg
     4561 gcttccgtca atctggtctg cctcaatttg cgattgctga cttggtgacg gatggaaatg
     4621 ttttggaggt agccagagaa gaggctgcag ccctttggca gataaaggat tggcagatgc
     4681 ttcctgagta tcagccttta gcgcaaagcc tccaaataga aaatcaggat aatcgttatt
     4741 tcgattagca gactgtgcta tactttatac tgatgacgga aaaaggagtg gaaacaatga
     4801 aaattgccgt agatgcaatg ggcggagatc acgcacctca agcaattgta gaaggcgtgg
     4861 ctttagcgca gcaagatttt ccagaagtag aattcttact ttacggaaaa gaagcagaaa
     4921 tcaagaaata cttaacaaat gaaaaaaaca tcacgattat ccatacagac gaaaaaatca
     4981 atagtgatga tgagccagta aaagcaattc gacgaaaaaa gactgcatca atggtccttg
     5041 ctgcacaagc agtgaaaaac ggtgaagcag atgctgtatt ttcagcaggg aatacaggcg
     5101 cgctactagc agcagggttg ttcatcgtag gacgtatcaa aaatatcgaa cgccctggct
     5161 tgatgtctac cttgccagta gtagggaaag aaggtgccgg tttcgatatg ctggatcttg
     5221 gcgctaacgc agaaaacaaa ccagaacatt tgttgcaata cggtatttta ggttcttttt
     5281 atgcacgtaa agtccgaggt atcggacgtc ctcgtgtcgc actcttaaac aacggaacag
     5341 aagcaacaaa aggtagtgaa gtgacgaaaa aagcctatga actccttcag aatgaaacaa
     5401 gcttgaattt tattggaaac gtagaagctc gtgagctact caatggtgtg gctgatgtag
     5461 tggtcacaga tggatttaca ggcaacgctg ttttgaaatc tattgaagga acagcaatga
     5521 atatgatgaa tctattaaaa tctgctattc taaatgaagg gatcaaagga aaaatgggcg
     5581 ctatgctttt aaaagacggc ttacgttctt tgaaagcaga aatggattat tccaaacatg
     5641 gcggtgccgt tttgtttggc ttgaaagcac cagtcattaa aactcatgga gcaacaggcc
     5701 ccgatgctgt tcgctatacg atccgccaaa tccacacaat gcttgagaca gatgtagttg
     5761 gacaattagt cgaacaattt gaaaaaagcg aagaatagta tttggatatc tgaagcaatc
     5821 cgttccattg aaaaaaaggt ggacattcct gaaaaatgcc agtaaaatta gccatggtgt
     5881 aggtaattga caagtgtatg attcttggag gtgaaaaagt tgactcgcga agaagtattt
     5941 aacaaagtag cgaaaattat ttcaaatcat tttgagatag ataccgataa agtgacggat
     6001 gaattgaaca tcaaagatga tttaaaagct gattcaatta gtattatgga attcgtttta
     6061 gagcttgaag atgaattcgg tacggaaatt tcagatgaag acgctgaaca aattgaaaca
     6121 gtcggtggcg cagtagatta tatcagcagc catcttaaat aatttcaaat aaaagcaaag
     6181 aaggataaaa agctgcatac agctttttat ccttcttttt t
//
LOCUS       DAYYGO010000130         6174 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000130 DAYYGO010000000
VERSION     DAYYGO010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.133"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            725..811
                     /locus_tag="ACHA5T_002458"
     CDS             725..811
                     /locus_tag="ACHA5T_002458"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFM8887996.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1622)
                     /locus_tag="ACHA5T_002459"
                     /pseudo
     CDS             complement(932..1622)
                     /locus_tag="ACHA5T_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1629..1907)
                     /locus_tag="ACHA5T_002460"
     CDS             complement(1629..1907)
                     /locus_tag="ACHA5T_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8887997.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1867..2781)
                     /locus_tag="ACHA5T_002461"
     CDS             complement(1867..2781)
                     /locus_tag="ACHA5T_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM8887998.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2763..3143)
                     /locus_tag="ACHA5T_002462"
     CDS             complement(2763..3143)
                     /locus_tag="ACHA5T_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFM8887999.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3593..3880)
                     /locus_tag="ACHA5T_002463"
     CDS             complement(3593..3880)
                     /locus_tag="ACHA5T_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM8888000.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3900..4124)
                     /locus_tag="ACHA5T_002464"
     CDS             complement(3900..4124)
                     /locus_tag="ACHA5T_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFM8888001.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4579..4749)
                     /locus_tag="ACHA5T_002465"
     CDS             complement(4579..4749)
                     /locus_tag="ACHA5T_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888002.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4764..5297)
                     /locus_tag="ACHA5T_002466"
     CDS             complement(4764..5297)
                     /locus_tag="ACHA5T_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM8888003.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5318..>6174)
                     /locus_tag="ACHA5T_002467"
     CDS             complement(5318..>6174)
                     /locus_tag="ACHA5T_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFM8888004.1"
                     /translation="IVSRMREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEK
                     ILSLRFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYS
                     LQQFNELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPI
                     RQELTAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALAL
                     ADKQKEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLL
                     SDLDNLFQ"
BASE COUNT         1634 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa atataacccc tggcaataaa
     1501 aacctcttaa atcgaatttt agagcctttt aatgaatatc ttgtttgata agccttatag
     1561 ctaagccaaa taattcctaa aatactggca aaaatgagta gatagggtaa atagaatgcc
     1621 atttctattt attctcccca taccagccaa aaggctaaca gtgtcatgat taagcaacat
     1681 aagggggcaa gaatatccat tgatcgctgc ataattactt ttttctgttc tttatttcgc
     1741 tggtttaata gcgcatggtg gaattttttg acttgtgaat caattgtagt ttgtattctt
     1801 tctttggtat aaggtgcaag aaaagcacgt tgtttcttta tttcattttc tatgtcttgt
     1861 aacgttttag aacgataatt cttgttggta gtctgctcct ctttcaacgt gttggtcatg
     1921 gtttcttcga gtcggtctaa tttccggttg ttcagtcgta agagttcgtt gttctcttcg
     1981 tacttctttt gcagtttcgc gaatgctagc tcgagctctt cgtagctcgt tggttcgttg
     2041 ttgattgtag gctcgttctc gtctgtccag ctcatgtaaa atcgtctcct tttcgtaatc
     2101 ctttcctagt ttgttggatc gtactttttt ctttttgtgg atcaattcgt aagtaaaatt
     2161 tttcccacga ttcaagcaat gaatgccgaa tgttttaaga gaatcacaaa attggttata
     2221 cgttcgagtt tttttgtttt tcattgtttg atcaatcgca aaccgaattt cgtctttcca
     2281 aacaggttca tttttttctt ttaatttgat ttcagacatg gttcgttttt cgggtttctt
     2341 ccctcgttca gtgattgtta agtcatgttc aagacaaatc tcgtcattta ggtcataaca
     2401 tttgtttaaa aatgcttgac catgggcttg aaatttgttc ccagtttcca gattgacgga
     2461 gttaatgaca atgtggttat gaatatggtc tttgtctgta tgtgtgtaaa tcgcgatttg
     2521 gtggtcaggg gctatttttt tcgcatactc tgcccctagt tgattggctt tttcagcggt
     2581 aatttctcct ggtcgaaaag actggatcaa taagtgtgct tgtacacgat cgtcttttcc
     2641 atacagcatg cgaatttgtt tcatttcgct tttggcataa tcaatgtcac agtttatccc
     2701 tgttttgacg gttgctcgtg gttcggcgta attgatcgct cggctacaag aagtggttcg
     2761 gcttacttta actgttgcca tatcttttgt agctcctttt gcatgatttt taggttttcg
     2821 gataagcgtt gggcgtcgcc aggatcaacg tcgtgttggt gtgtgttgca ccacttggca
     2881 atttgattga tattcccgcc agctttggct aaatcgcgcg cgattgcctg agcaatgtct
     2941 ggcgccactt tcggtgtcac cagacgcgcg ttttgtgcct tggctttcac gaaagccggc
     3001 acactcattt gcaaagcttc agctgactgc ttcagctttg caaattcgga ttcgctcact
     3061 cgaaaactga tttgttttgg ctcggatcga taggtatatt ttttagaatg attgctagcc
     3121 aaatattgat tttgttcgct cataagtccc accctttcag tacgtcctac ggacgtatcg
     3181 ttcgcaatac cattgtacct aaaaaggtac aataagtaaa gctcactaaa gctcactaaa
     3241 gggcttggca agctctatat ttgctagcca aatacctacg tatttggtgc aaataaccgc
     3301 ttgtcaaagg ggcgcaagct cccccttgaa tccccctccc ccccactctc cacaatacaa
     3361 aaaagactag caaatgctag ccactttttg taaagtttcg cttgtttcac cttcggtgaa
     3421 attgttgcaa atcaataatt aggtagagct acattttttt cgttgttcaa agattcttat
     3481 gccatttact attaataaat ctataaatat gataattttt cggctctata atttatggga
     3541 ctgatgaatc agaattgatt cttcagtccc attttcattt tattccaaag aattaactcc
     3601 aaataccaat agaagcccat cctctatata cttgagattc cttactaaca atttcttgta
     3661 attctattaa agatttaggt aatccaaatt tatgttttaa aatatatctt ccaagaaaac
     3721 tacttaattt catactaact tcaccaacac tttttccctc actgatttct ttagaataaa
     3781 gaaagagctt ttcttttaag tcaggatagt catctatagt tgcttcattg tatactttac
     3841 taattgcatt taaaagtttt tcttctttag taaaatccat tataataacc tccctaccac
     3901 taccttctag gtgcccatgg accatgatta acccaaccat taacaataat ttttccaatt
     3961 tctcctttag cttgattcca atctacccaa catttttctt tgttacaata aagaccattt
     4021 ccatagtaag tagctgcatc aacttttatt cctgtaccga tgccagctag acaagttcct
     4081 aatattccta gaataacaca atgttttaat actttctttt tcatgattgt ttttcctttt
     4141 cgttttttta aaattgcttg ttatattaaa taatcttagt aattttatta tataacattt
     4201 tattatataa caatgattaa cgtcataaaa agtagttttg aattcataat cgtttaaata
     4261 aaattataag atcagttatt taaacagcta gtagacataa tgtgtatact accaagtaac
     4321 gcctgtacca agggatccag gcgctgattt tgtgaatttt tattggtcta ggttaaaagc
     4381 tctttttaag gcttggtttt ctgcggttgt ttctttctaa ttctttgtga atattgatcg
     4441 gtcatttttt aaatacttgt tatttttgtt tgtgtttgtt atgcttttta catatctcat
     4501 attcatttat tattcttttc taaggttaat aataggataa tctctttcag aaagactgct
     4561 cgtacgagca gtcttttctt atttatcaag tttttctaca aatacttcct cattttgact
     4621 gttgtagtaa ttaggattga gttttagttc ccctttttta tttcctttgt tggccactga
     4681 taataacatt gggctaaagc ttaatattcc tagtaggata gaaccgaata gcaagtgttt
     4741 ttttgtcata agtatttcct tcttcatttc catagctgcc accatttttt ccaattcttt
     4801 gtttgttcgt ccattgtttc tgttgcttca gaaagagtcg ttgaactatt ctctgaagac
     4861 tcttccgtat aagtcaactg cagttgctcc tgaagttttt cgatttgttg gttggcttgc
     4921 agggtgagtt gttgttgctg gtcgagtagt ttttgtatat gagaattatt ttgctctttg
     4981 agggcaagtt gttctcgtaa aaattcattt tcttcacgca actgatctag caacttgttt
     5041 gcaaccacgt ttttgtttgg tatataagca tttttatcgt tgttttgttc atgtttatgc
     5101 aacttatcta gcaacctttg attgtctaag ttgcgagcaa gttgcaatac tccagaacgc
     5161 aatacaacct tataaccacg ctcattttta gctatcatgg aagaatctaa tttttggata
     5221 tgtttcgata ttgcttgcct actcatctta aaaagatctg acaattcctt tattgtaaag
     5281 gtttcttgtg gttgcatact ggttgcctcc taggttgcta ctggaataaa ttatctaaat
     5341 cagataataa actttttttc tctttttccg aaagtgcgga ctctgtttgt ttttcaagag
     5401 cttttttctc tttttctagt tgtttgattt gttggaggag ttcaagttct cgtttcttct
     5461 gtttgatttt ttcgttaatt gttgtagcgg gttcttcgta ttctttttgt ttatcagcta
     5521 aagccaatgc tttattttgg tcttctttgg ttggtgcatg tttgttaaag tcgtctgctt
     5581 ttttgatctc aggtttgaac gtaaattgat agccaataac aggttttccc cgtccttttc
     5641 cgtatttctt tttgattgtt agtcctttga agattgcagt tagttcttga cgtataggtt
     5701 ttataactcg tttatctata tcaggttgct tataactttt aggaatatct aataattcaa
     5761 gaaagtcttc tttggagaaa tatgcgaaac ctttggttct gtattgcttt aacagacgaa
     5821 acatagtctt tgcatagcca ctttgtagct cgttgaattg ttgtaaagaa tagcgtaccc
     5881 actcgtctaa attatttaac aaaggcagtg ctttttcgta aattcgtata tctacgtaag
     5941 gattttctac gtccccgttg attttaaatt ctgtaaacat tacaaacata tcgtaagaaa
     6001 gcccagactc acttctacga ccaaagcgta aagataagat tttttcataa gttgctttta
     6061 aatcttctac aaatcgtaca tttgcagttg ctttgtagtt acttaaatct tttagttggt
     6121 caaacgtaaa acgaactgtc ttatcccctt tttcacgcat tctagacacg atgg
//
LOCUS       DAYYGO010000131         6154 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000131 DAYYGO010000000
VERSION     DAYYGO010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.134"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(137..1099)
                     /locus_tag="ACHA5T_002468"
     CDS             complement(137..1099)
                     /locus_tag="ACHA5T_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8888005.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1101..2540)
                     /locus_tag="ACHA5T_002469"
     CDS             complement(1101..2540)
                     /locus_tag="ACHA5T_002469"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFM8888006.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2725..4671
                     /locus_tag="ACHA5T_002470"
     CDS             2725..4671
                     /locus_tag="ACHA5T_002470"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFM8888007.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            4935..5807
                     /gene="scrK"
                     /locus_tag="ACHA5T_002471"
     CDS             4935..5807
                     /gene="scrK"
                     /locus_tag="ACHA5T_002471"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFM8888008.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT         1958 a         1051 c         1093 g         2052 t
ORIGIN      
        1 atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac aactcctttt
       61 tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt atagacatta
      121 aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt aaaatataat
      181 ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata agacttgcaa
      241 tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca atgaaagagg
      301 taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt tgatgagcaa
      361 ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct ggtttgtgat
      421 ttataatagt tgactttatt tccatttcac gacggatagt agacagatta ttaggaattt
      481 tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat tctgtgggag
      541 aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt ttttgtaaag
      601 tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa acattaggag
      661 ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca gcaattccaa
      721 gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt tcaatgtatt
      781 ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat ccatgtttaa
      841 ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta atattaggaa
      901 aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa ttatttggtt
      961 tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct gataaatatc
     1021 ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat tcagcgacat
     1081 cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt aggttcgcct
     1141 gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt aaacactttt
     1201 tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt agcagttgtt
     1261 tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata ttgaatgcct
     1321 gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa gctaagtcca
     1381 tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg aaaagttagt
     1441 tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga actagcacgt
     1501 aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat tgaaagttct
     1561 tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc gatatcaggt
     1621 agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc ttgagtcgca
     1681 tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag ttttactttt
     1741 tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc ataatttagc
     1801 tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc tacaaatact
     1861 agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag gttgccaact
     1921 tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa gcctgatttt
     1981 tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa catttgtgga
     2041 tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc acctttcgta
     2101 atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc ccaattttca
     2161 tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata tgcagaaccg
     2221 gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc tgttacatga
     2281 aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg agcagctcca
     2341 aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa accattagga
     2401 tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg tgatcgtttt
     2461 acattatcct taattttatc catctcatct ttagtccaat cagaataagc acgatatcgt
     2521 atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa ctagtatggt
     2581 aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat atgacaaacg
     2641 tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc gttttcacgt
     2701 aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga cgtaatacaa
     2761 gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct tcgtctcgta
     2821 ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga tgtgaaaagc
     2881 acatttaaaa ttgatggaca atatcaagtg attatcggtg caggtgatgt taatttcgtt
     2941 tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga tttaaaacaa
     3001 attgctagta aagataaaaa attcaacccc attatggcat taattaaatt attatcagat
     3061 atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc attacaaaac
     3121 tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta tcctgcgatt
     3181 gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt cttaccaatt
     3241 ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg tgctgcaatt
     3301 ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa tatttttggt
     3361 tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc agctgtttgg
     3421 atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat tgactttaca
     3481 ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt tattggccct
     3541 gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata taacactact
     3601 ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat gactggttta
     3661 catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa tggtatcgga
     3721 catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg tgctgcaaca
     3781 tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc ctctgcgggt
     3841 gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt aaaataccgc
     3901 tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc aggattactt
     3961 aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc aattaatgca
     4021 acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc ttttgcaatt
     4081 acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga aattgctgaa
     4141 caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac tgatgttgaa
     4201 tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg taaagtgatt
     4261 agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa agggattgcc
     4321 attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca actcacgttt
     4381 gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct aattcatatt
     4441 ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac tactaatcaa
     4501 aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga gaaagaaggt
     4561 cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga agtaaatata
     4621 gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata aatatatttc
     4681 cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat caaatatttg
     4741 agaataacat tttatttaat cttttcatat atcaattgta tgaaaaatta ttagaatttt
     4801 aaaaaatgat aaaaattcaa aaagaaaaaa tagtagttat ttacttgtca tgttactaaa
     4861 taaaaaaata atttaaatat gatatatagt catttattta gtgatttcat tctaaaataa
     4921 agggagaaat gtctatgcta tacggtgcaa ttgaagcagg cggcacaaaa tttgtgtgtg
     4981 ccattggcga tgaagaaatg acgatcaagg aaagagtgag ctttccgaca acgacacctg
     5041 aggaaacaat gcctttagtc attgacttct tcaaacaata ccaagcagat ttagcaggga
     5101 ttgggattgg ctcatttggg cctatcgata tccatcgcga ttccgccact tatgggtaca
     5161 ttacctcgac cccgaaattg gcatggcaaa attttgattt catcggtacc atgaaaaaag
     5221 aatttccgat cccgatttct tggacaacag atgtcaatgc ggctgcctat ggtgagtatg
     5281 tcttcggcag tggcaaagga ttgagcagtg tcgtttacta tacgatcggt acgggtgttg
     5341 gcggcggtgc cttacaagaa gggcgcttta tcgaagggtt tagtcatcca gaaatggggc
     5401 atatgctcgt cgtccctcat ccaaaagatg actttgctgg cagctgtcca tttcatggca
     5461 actgtttaga agggatggcc gctggacccg ccatcgaaaa ccgcttaggg aaaaaaggac
     5521 aagaggttgc agaagatgat ccttattggg aaatcgaagc ctcctatatt gctcaatgtg
     5581 cctacaatac aaccttgatg ttttctccag atgtgatcat ttttggcggt ggtgtgatga
     5641 aacaacgcca tatgctgcaa aacgtccacg acgcatttgc aaaattggtc aatgggtatg
     5701 tcgaaacacc tgagttaagc aaatatatcg tgaccccagc attagaagac aacgctggaa
     5761 cgctcggttg tttggcattg gctcgagaag cggtcttaca ttcataaacg aaaaaaggct
     5821 cttcgacatt tagtgttggt agagaaagat acaactgaaa atttagtcat aaccagttag
     5881 gcagagtgct taactggttt ttgttttact tggaagatca agccttgaat gagataaatt
     5941 cttgctcgaa agttatggaa atttcgatag ccgtaggcca cttgtattgt gcaatagttt
     6001 tttacaaaaa aaagggggtg cggatatttt tttggacctt tttaacaaat aggttctgtt
     6061 gcaaagtttt aaatctacta tcagataagg tagaataata gaaaaagata gcaggaggaa
     6121 tgacgatgag tcattttaaa ggaaagcaat ttca
//
LOCUS       DAYYGO010000132         6095 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000132 DAYYGO010000000
VERSION     DAYYGO010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6095
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.135"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            80..1195
                     /locus_tag="ACHA5T_002472"
     CDS             80..1195
                     /locus_tag="ACHA5T_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888009.1"
                     /translation="MKNYHLSCSLKKGNPIEGEEYRIRYFFHLMYWQSFDENHSDILS
                     SIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSITYVPEEMNKFVHPL
                     ITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYLEEDLLDTPDKVQES
                     WGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGTKKKVDAFGKSAEDR
                     DFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCMLVRDIFIKHEQPVIF
                     YIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYPIDKKAYDQTNTQIF
                     YWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(1221..1661)
                     /locus_tag="ACHA5T_002473"
     CDS             complement(1221..1661)
                     /locus_tag="ACHA5T_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8888010.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            1821..2327
                     /locus_tag="ACHA5T_002474"
     CDS             1821..2327
                     /locus_tag="ACHA5T_002474"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFM8888011.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            2495..4408
                     /locus_tag="ACHA5T_002475"
     CDS             2495..4408
                     /locus_tag="ACHA5T_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8888012.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            4413..5414
                     /locus_tag="ACHA5T_002476"
     CDS             4413..5414
                     /locus_tag="ACHA5T_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8888013.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(5616..5822)
                     /locus_tag="ACHA5T_002477"
     CDS             complement(5616..5822)
                     /locus_tag="ACHA5T_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFM8888014.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
BASE COUNT         2254 a          930 c         1210 g         1701 t
ORIGIN      
        1 aatccttaga aaatcttagt caaaaaatta tataagtttg cgtacgattc aaagaaaatt
       61 aagagaactt aatactattt tgaaaaacta tcatttatct tgcagcttga aaaaaggtaa
      121 tccgatagaa ggagaagaat accgtatccg atactttttt cacttgatgt attggcagtc
      181 attcgacgaa aatcattctg atatattatc aagtataaaa aataaacgtc aactattaga
      241 aaaatttgcg aaacatgcgc catatacgcg tagtatagat agaaagaaat ggatcagcct
      301 actaagaatt tcgttgcaac gaatgaaaca aggccactcg attacgtatg tcccagaaga
      361 gatgaacaaa tttgtgcatc cattgataac aaaagaagag tttacgcatt acatactaga
      421 gccttttttt gaagcaaact tctggtcgat gaatcacatc actccaacag agcaagctta
      481 cctttatttt atgtttggtg taatgaacac ctatttggaa gaagatttgt tggatactcc
      541 agataaagta caagaaagtt ggggctggat tgaatcgtct attgtagaaa attttgaaaa
      601 aaacttcaag atttgtttta caacttcaga aaaaaattat ctatatgtca atttggcaat
      661 gatccatttg tattcaaggg tttttgggac aaaaaagaag gtggatgctt ttggaaaatc
      721 agcagaagac agagattttc agcatatatt tccagtgatg tatccaatca tgcaacagtt
      781 ttttagtggg gtagctcgca gaattccaga gcttaatact tattatgaaa agaacagacg
      841 gttgattttt caatactgta tgttagtcag ggatattttt ataaaacacg aacaacctgt
      901 catattctat attcaatcaa agtatggtaa aacacaggaa ttatgggcaa agaaaaggat
      961 attatctatg actgaacgcc caataaaata tagtgcatcc gctgatcagt tacctgatat
     1021 tgtcattagc gattatccta tcgacaaaaa ggcatatgat caaacgaata cacagatatt
     1081 ttactggaat ggacaaccta caaaaaatga ttgggcgcgt ctgaaagctg aaatcataaa
     1141 aatcaggaat gagaaaaata gatccatatt ggaaaaattg gatttcgtta attaagaaaa
     1201 gcagattgtt tatgccaaaa ttaagacatt gcaaaaagat tattttcttt aaaatcgaca
     1261 agatgaccag aaagcaagag ttcgttgagc tcttgctttc ttttagtttt agaaaggaac
     1321 atcctgattt tatcttcgct aaatccagtc aaaagttttg cagattggtc gtagataatc
     1381 ggttttttca ataaagaagg ggatgtacga atctcaatat atagttcact caaactgagt
     1441 tcatctattc tgtgaacaag atttttataa ctaatagaat gagtagagag catttcggtc
     1501 acgccgttgt ttgtcaattt tagtaagtgg ataaattcat ttttggttag tggttgacga
     1561 attaagttct gttcgataaa cggtatatca tggacgataa gccaacgttt tgcgcgtctt
     1621 gaagacgagc agccagaacc tgtatacaat ttaatcatca tacattcctc caatagctaa
     1681 gtatttatga aataataaca tgaagaaata gtcgaaaagt gccatttaga ggtgtaaaat
     1741 aagaaaatgt tgtcacgtat aatggaacta gctagataag aaatactatt aaatcaagaa
     1801 aaaagaaaga aggcacaagt atgtcaaaca cagccgacgc aatagtagtg atcgatttac
     1861 aaaaaggggt atgttttgat tcttcgccga tacacgaatt agaaacagtg acagcattag
     1921 tgaataaaag aatcaaaaca tacgaaacga ttgggaaacc agttattttc gttcaacacg
     1981 aagatgctca attagtgcca caaacaaaac catgggagct attgtcggaa ttatctattc
     2041 ctgagcacag ttattttgtg agaaaaaccc atgcgaattc attttataag actaatttga
     2101 gtgttttatt aactgaattg gactgtcatt cgatcgaatt atgtggggca caagcagagt
     2161 attgtataga tacgactgtg aagtttgctc atggattagg ataccagctt atgatgtgta
     2221 gaggagcaac gacaacttat gataatcctt ttatgaaagc aaaggaaaca atcgcttttt
     2281 acgagaaaat ttgggataaa cgatttctga catttttgga gcaataagtt gtttcttcgt
     2341 ttaaaaaaag cagaatggat actggtttat ccattctgct ttattacgta aaatgagaaa
     2401 ataatcctgt tctattttta tgggataaac catttatttt ttgtataaaa aatgtttgcg
     2461 tttacatata attctcctag aaacataaaa aggagtgaat agcttgagaa caaaacaagt
     2521 attaaaggga gcgttggtca tcttatttgc tgtaagtaca agcgtcccgc aatggacagt
     2581 atatggagag agcatttctt ccgaatcgga atctcgggag gtagaaacca cagaaactac
     2641 tgatgctgga aatgaaacga aatcgagtac ttcacaaaca gaaacaaata ctgaaacaac
     2701 ggttgagaca tctgttacta gcacagatca agaaacgacc gctaccacag ataaccaaca
     2761 aacagaagaa gcaacagcta caaaaatgtc aaacgacgaa tcctcttctg ctaaagtaag
     2821 tgacaaagag aatgggacag aaaatgatga tatcggtaaa ggtgaaaatt tcaatggtaa
     2881 agaatatttg aattacttcg gtaaacagat gacagaaata cttaatggac caacagaaga
     2941 catcaagtca aaattaatag agttattagt cactgagctt gctgttaatg attatggtgt
     3001 agataaagag attatcaaac atctgattgc tacttatcta ttgtacgata tcgcttttgc
     3061 aaaaaacagt gtgttgatgt cgatgcttgg aggtacagat gatgggacaa cgcctacaga
     3121 aaacacaggc ttctatccaa atacaaaaca acttcatcaa actgtactaa acaaacaaga
     3181 tggcaaaatg atggatgtat atgcatatta tgtcgatcaa ggttcagata aaacagtcat
     3241 gatccatggc ggatttcgag ggaattggaa taacgggatc gttacagaag aatacaatga
     3301 tttttataag gccgggtata atttattatt tgttgattcc agagcgactg gtaatagcgg
     3361 aggcgattat gtgacctatg gacaatatga gtcagatgac gtcctttact ggataaatca
     3421 agaagttcga gagcggccat ctcaaaagat tttactttat ggtggttcga tgggagcagc
     3481 tacgatgatg tctgtattag cgaaagatat tcctgtcaat gtcaaaggaa ttattgaaaa
     3541 ttgcggattt gcatcaattg atgaacaact acgttttacg tacagccaga cagtagcccc
     3601 cgcactaccg gatgccataa aaaatcagtt agacatcatt ggggatcaag agcatgaaga
     3661 tttatttatg ggccttttga aacaatacta ttttgatcag gagatgcatt tggatacaaa
     3721 agctgctcta cctacgattg gaatgtcaga ttcattgcct aaattgatta tccatggaac
     3781 agcagatgat gtagtacctg tgtctaacgc gcaaaaatta tatgagttat caggagggta
     3841 taaagatctt ttattagtag aaggagcagg gcatggaaag gcacaggaag ttgaccatgc
     3901 tgcctacacg aaacatgtaa cggacttttt gaaagtagtg ttccatgatc agataaacgt
     3961 aaagtatgtg gatgaaaaca ataaaagtct actcaaagac caggacgaaa tccagcttta
     4021 cggagcttat ggagaaaatt atatgacaga gcaaaagaca ttcgaaggat acgaattagc
     4081 aaatgttgaa ggaccgactg aagggatttt taatgagacg ataccaacta ttattttcta
     4141 ttacaaaaaa atcccagtag ctccaccaaa aaagcaagat ccgacagaga atgcagacaa
     4201 taagggaaag aatgaacctc gagaggatca atcaagtgat gatacgcaac cgattcagat
     4261 gacagtgaaa gtacaaaaaa agacagatgc aaaaaaaaga atgttgccga aaactggaga
     4321 aaagaaacat tcgcttctca tttattcagg tggattttta tctgttagca cgattatcac
     4381 atggatttgg cgaagaaaga gagggtaaat gaatgaaatg gttcaagcga atagtgattg
     4441 cagtaccagt tatcatcgta gttattttag tcactgtatt tttgattaat caaatcacac
     4501 caaagccgat tagcttgctt gttagaaaac aatttgacac aagtgaaaaa gaacaagtat
     4561 atgcccgacc agatgatttc cagcaaaaaa tcgaacaaat cgatataaaa aaagaaatat
     4621 cctatccttc aaagtacaaa aataataata tggatatcta cacaccgaaa gtggaaaaga
     4681 aaaaattacc gattcttttc tggatgcatg gaggcgcata tgtaggaggg gataaaaatg
     4741 attgtcgtga ttatttggaa tatctatgtt cagacacgca gcaaatcatc gtaaatatag
     4801 attatgaacg atcaccggaa gcaaaacatc ctacaccagt tatccaattg aacgaagcca
     4861 ttcaagcagc taaaaaagag ataaaagatc aagcagattg gtcgaatatt tcgattggtg
     4921 gaaattcggc aggtgcacaa attagtggag aatatttgtt agcacttcaa aatgaagaaa
     4981 ttaaaaaagc tgatcatctt gatccgactt tggagaacaa gcagattacg aagtttattt
     5041 ctttgtctgg tctgttggat ccttctgcac ttactcaagt aagtgataaa atctctagct
     5101 ttctttatga aaaatgtgga tgggcttact ttgataataa ggactttgaa caatcagaga
     5161 aggtcaaaag tttagccttg gtcagacatg cggatcgttg gacgcaaaac acattcttaa
     5221 cggatggaaa tacaaatacg tttacgaaac aaatggaaga agtaacttct gtatttgaga
     5281 aagcgggggt gaaagtcaca aaagtagatt atgcggagaa aaatgctgtt ttgaaccatg
     5341 aatatcagtt tgatttcagc aataaacaag cgcaagaaac atatcaaaag ttagtaatgt
     5401 ttttcaagga gtaaagtctt atgatgcaaa agaggttgta atctgtcact tagctccaag
     5461 tcacaaggag caatttcaat aaacaagctt ttcatggttt atgaatattg taacttgtga
     5521 ctgaggagtt agagatggca aggggcacct tttattcgaa atttgggaat atcacacgat
     5581 tattgtcacg atctctttta cgtaaatttt ataagtcaga tttgttgttt tgatcgttta
     5641 aaaaaagcaa caaatccgaa gcctgcggag gcaagaaggg acaaaaagcc gagtggtaag
     5701 caaaagaatg ctggttccac taatattccg ttgttattga tataaggctg ggaacaaaga
     5761 tgaatagcaa ataaaatcaa tgcactagcg aaaaaaagca aagaaatatg gatagctttc
     5821 ataagcagca cctccttttt tataattgta tgataaatag attaagaatc tataaatgtg
     5881 acaagtttgt taggatgtga ataaaatgat catctagcga acttgaaaca aagatggcta
     5941 atgataagca attaatgatt cagcaatcag ataggtacaa agtttaccat catacgtcta
     6001 gaccaataat agaaaatatg ggaatataat agaaaagatt taggataccc ccaaaagaat
     6061 ttccccaaaa attcgatgta tcctaaactt tttta
//
LOCUS       DAYYGO010000133         6068 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000133 DAYYGO010000000
VERSION     DAYYGO010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.136"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            832..1566
                     /locus_tag="ACHA5T_002478"
     CDS             832..1566
                     /locus_tag="ACHA5T_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8888015.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1582..2751
                     /locus_tag="ACHA5T_002479"
     CDS             1582..2751
                     /locus_tag="ACHA5T_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8888016.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2767..3762
                     /gene="lacD"
                     /locus_tag="ACHA5T_002480"
     CDS             2767..3762
                     /gene="lacD"
                     /locus_tag="ACHA5T_002480"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFM8888017.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3841..4767
                     /locus_tag="ACHA5T_002481"
     CDS             3841..4767
                     /locus_tag="ACHA5T_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFM8888018.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4786..6036
                     /locus_tag="ACHA5T_002482"
     CDS             4786..6036
                     /locus_tag="ACHA5T_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8888019.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         2027 a          997 c         1246 g         1798 t
ORIGIN      
        1 attggcgcag cgaatccttg ataatgagct cggatgtgct aacgctagcg aggcagtcta
       61 actttaactg ttagctcctg ccgaagatcg tcacatccga ggaggctcat tgtcaaggac
      121 aatcggaata gcatacggaa agaagttctg caccttataa aatttgtcaa aaaaactgtt
      181 gccattccaa agtttgaaaa atgctatttc gaggtagaag gatattatat aagcacggtt
      241 gactaaacga agtgataata aaaaaagtta ttcaagaata agaaaaatta tgatacgaat
      301 tcagataaac tggagaatac agagaccttt aaaagggata agaaacaaca taaattcctc
      361 ttaagagaga acgaaaggac aaagccagca acttgataca cgagctagta ttagaggaat
      421 ttattctcat agactccctt ttgaatacgt ttcaacttta attttcatgt tcataaatga
      481 tttgaaacca aagcaaatgc gttgcacttt tcctccgtct tccgaaaatc gttcaattca
      541 ctggtaagta gtcatttgat agtttgaggc aaattgttga ttgatgaagg gtaggagcta
      601 aaaaaagata ccagttgaag agagaaaaag aaaaaaatag agggtgttct tgaatgaaaa
      661 aagttggaaa attatttttc atttcctttt attcctatta aagtcagtga tgtcaatcat
      721 attaacaaga ctaaaaatga aaataaaaaa aagattgttc ttagtattga ctcataaaaa
      781 ataccactac tatacttaca ttgtggttat agccagttag gagagacatc gatgcaaaat
      841 acctttcaat caaaatcact ttattatcag ctggtagaaa tattgaaaca acgaatcgaa
      901 aacagtatgg tcgctcatga caagctgcca tcagaaagag agttgaccag tttttatggc
      961 gttagtcgca cgacggtgcg actagcattg caagaactgg aaaaaatggg ctacatttat
     1021 cgacaacatg gaaaaggaac ctttgtttcg gaattaagga caaatgcgac tgatctagct
     1081 ggcgcatata gttttaccga acaaatgcgg gcaatgggca aacatcctga aaccaagata
     1141 ttggccttca agaagattga agcaactaaa tatttagcga gtcatctgaa tgttcaacta
     1201 ggtgagcctt tatttcaatt agaccgattg cgcttagctg atgatttgcc aatgatgatc
     1261 gagaaatcct atttgccagt gaagctattt atgtcccttt ctcaagaaat tctatttctg
     1321 aaacctttat ataatttgtt ttcagagaat ttcaatcaaa tcgtacggtt ggctgacgag
     1381 gaattttatg ccagcattgt ccagaaagaa gatagtgagt tgctaaatat tccagaagga
     1441 tcaccagtgc tccatttagt tcgctcaacc tataatctcg agaacgaagt gattgaattt
     1501 acattaagtg tagcgcgggc tgataaattt cattataaga tcaggcatct gcgttccact
     1561 aactgaattg gaggaaagaa aatgtttatg ttctcaaaag aagaactacg aaagctagga
     1621 gcagaaaata ctacccgaga aattcgacaa caaccggaac tgtggcatga gacgtttgac
     1681 cgttatcaag aaacagttgc agatttagat cgtttgttta cggatgcgaa acaacatgct
     1741 ggagagcgac caatccgagt catttttaca ggggccggaa cctcgcaata tgtgggagat
     1801 acactaattc cgtttcttac tagtcaagga aatacgaacc agtttatttt tcaaagtatt
     1861 ggttcgaccg atatcgttgc tacgccaaaa gaatacctga ttcctgatga gacaacgatc
     1921 ttggtctcat ttgcccgtag tggaaatagt ccagaaagtc tagccgcagt taagatggcc
     1981 aatcagctgg tcaacaagat ctatcatatc gtgatcacct gtgcaccaga cggattattg
     2041 gctacacaaa tgcctacaaa aaaagaaaat ctagttttac taatgccaaa caaagccaat
     2101 gacgctggtt ttgctatgac aagcagtttc agctgtatga cgctgactgc gctacttatt
     2161 tttgatcaag caagcaatca gcaaaaagaa agatatgttc atcaattaag ccgactagga
     2221 gaagaagtca tccaacgaga atcagagatc caagggtatc tggcatcaga ctttcatcgc
     2281 atcatttact taggttcggg aagcttaggc ggattgacta gagaagcgca attaaaagtt
     2341 ctcgagttaa ctgctggaaa aatcgcaacc gtatttgata catctttagg attcagacat
     2401 ggtcccaaat cttttgtcaa tgagcaaaca ttggttttcc tgtttgtcag caatgactca
     2461 tatacgcaga aatacgatgt agacatgctg gaagaaatca aaaatgacgg tattgcccaa
     2521 cgcgttgttg gaattggtca aaagggttca ttgtttagtg gtgattcctt tgaattagtt
     2581 gcagattcgt tactgccgga tggctatctt gctttagcag atatcatggt agcccaaacg
     2641 atcgcattac tttgttcggt gaaggttggt aatacaccag atacaccatc gccaagtgga
     2701 acagtcaatc gtgtggtcaa aggcgtaacg atttatccat atgaaaaata ggaaagagtg
     2761 tgaagcatgt tagaatggtc ggaaaataaa agacaagcaa tggaacgtct atcgacgcca
     2821 gaaggcatta ttaccgcttt ggcgatcgat cagcgaggcg cgctgaagaa aatgatgtcg
     2881 gctttaggga tggaagcgac aaaggaaaaa attgagcagt ttaaaacact aatttcccaa
     2941 gaattgactc cttatgcctc agcaatttta ttggatcctg aatacggact gcctgctgca
     3001 cagaagcgtg cagcagcgag cggtctgctg ttggcatatg aaaaaactgg ttatgatcaa
     3061 accgtacccg gccgcttgcc ggatctgctg tcggactgtt cagttctgcg attgaaagag
     3121 ttaggtgcgg atgcggtgaa atttttactg tattacgatt atgaagaagc agaggaaatc
     3181 aatcggcaaa agcaagtttt tgttgaacga attggaagcg aatgcatggc tgaaggactg
     3241 ccattttatt tagagttatt gtcatatgat gctaaaaatg tagatacgaa atcgctttcc
     3301 tatgccaaag caaaaccgcg caaagtgatc gaaatgatga aagaattctc gaaaacccag
     3361 tataaagtag atgtcttaaa ggttgaagta ccagtaaaca tgtcatttgt tgaaggaaat
     3421 gcaacagggg agatcgccta tacacgtcaa gaagctgccg cttattttaa agaacaaagc
     3481 gatgcaacag atttgccgta tatcttctta agtgctgggg tatcagcggc attattcatg
     3541 gagacattgc gttttgccaa acatgctggg gcacgattca acggtgtact ctgtggcaga
     3601 gcaacttgga aagatgcagt cgctcctttc gttgaaaaag gagaagcggc aactttagaa
     3661 tggctgacag aacaagggac acaaaacatt cgtcaattga atgaagtgat aagagaaact
     3721 gccatcccat ggtatgagaa agtaagtgaa agcacttgtt aatgtttcta atatattagt
     3781 agtgataaga gatatgtgtg attataattt atcattattg aaactgggag gatattttat
     3841 atgaataaaa gattgttaga ttattcagct tcagattttt tgaaagctaa gccgatggaa
     3901 ttaaagcagg caattttggc ttctgaaggt agaacaatta tggcagaaaa tgtgccaagt
     3961 agctgtcctt acatgggaaa atcagtgaca aatgcagaat tggagagagc tgctggagct
     4021 gatttaattt tgtttaatgc actagatctt tttaatcctc aaatttcagg tgtaccttta
     4081 actctaaaag agaatccaat aatctggatt aaaaaagctt taggaagagc tattggtgta
     4141 aatttagaac ctgtagacaa agaagttttg atgagtgaaa atcgaactga aatttcaagt
     4201 ggtagaatcg tttctcctga gactttaaaa aaggcaaatg aattaggctt taattttatt
     4261 tgtttaactg gaaatccagg aacaggagta tctaatagaa ctattgagaa agcaataaca
     4321 ttatccaaat caatttttaa tggattaatt atcgctggaa aaatgcatgg tgctggtgtt
     4381 gatgaaccaa ttatgaactt agatatagca aaaagattta ttaaaacagg tgtagatatt
     4441 ttattagttc ctgctccata cactgttccg tatttcatgg aaagtgattt gcgaggaatc
     4501 gttgattata taaaagattt taatagaaat aaatctattg aggaaaaagt tctagtacta
     4561 acggctaatg gaacaagcca agactcttct gaccaagaaa ctattaaaaa aattgcatta
     4621 gcatcaaaag catgtggagc tgatattcaa cacatagggg attctttaaa tggtatttgt
     4681 cttccagaaa atatttttgc tttgggtcaa gcaattcggg gatatcgtca tcaaattatt
     4741 atgttaggaa aatctaatta tcgttaatta aggaggaaat gaaagatgat cgacaaagtt
     4801 caaaaaatag ctgcaaaaat tagtaatcaa agacatctac tagctttaag agacagcatg
     4861 gctgcagtta tgccattaat cattattggt tcctttttta tgttattagc aaatttcccg
     4921 atagaaggat ttacaaaatg gttatctgaa attggagtac aatccttttt gaacaaagca
     4981 tcagattcaa catttggtat tattggatta gctgttacct actctttggg acacaatcta
     5041 gcaaagactt atggtgtaga ctcattatca gcaggattat tatctttgag tggatatgtt
     5101 ttacttacac ctttattgga tgcagatggt tctataggat ttccaacaaa atactttggt
     5161 acctcgggtc tttttgttgg tattattgta gcacttgtat ctacagaaat tttccggtgg
     5221 tttgtactta aaaatattac aattaaaatg ccggcaagcg ttccaccaaa tgtaagccga
     5281 gctttcgttg ctattattcc tggctttttt gtcgttctac tatggtttat tgttgttgta
     5341 atttgttata aacttggaat tgaaaatgtt catgctctta ttgcagatac actcgcaaaa
     5401 ccactatctt tactgacgaa aactttacca ggaattatat tggttatact tattcaatgt
     5461 ttcttttgga tgtttggtat tcatggtgca caggtaacgg ggccaataat tgaaccgtta
     5521 ttattgcaaa actctgatgt aaatagaatt gcttatcaag ctggaaaaga acttcctaac
     5581 attataactt atgaattctt atataacttt gttttttctg gtggtgcagg ttgtttattt
     5641 gcacttgcca ttttaacatt tttcttttca aagagtcagg aaaataaaac tttaggtaag
     5701 ttatcaatcg ctccagtaag ttttcaagta gcagagcccc ttctatttgg gtttccaact
     5761 atattaaata taaaaatgtt aattccattt gtaactgcac cagtagtaac aacattaatt
     5821 acttattttt caatgagtat gggattagtt gcaaaacctg taggagcaac aatcccatgg
     5881 acaactccac cgataattgc cggattttta gcttctggtg gacgaataag tggtgcagtg
     5941 attcaagtaa taacaattgc aataaatgtt ttgatatact atccattctt taaattagat
     6001 gataatgcca agctaaaaag cgaaaaaaat gattagaact acctatatgt ttctaactgt
     6061 agttaagt
//
LOCUS       DAYYGO010000134         6038 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000134 DAYYGO010000000
VERSION     DAYYGO010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.137"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(136..516)
                     /gene="yajC"
                     /locus_tag="ACHA5T_002483"
     CDS             complement(136..516)
                     /gene="yajC"
                     /locus_tag="ACHA5T_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFM8888020.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(613..1758)
                     /gene="tgt"
                     /locus_tag="ACHA5T_002484"
     CDS             complement(613..1758)
                     /gene="tgt"
                     /locus_tag="ACHA5T_002484"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFM8888021.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1976..2674
                     /locus_tag="ACHA5T_002485"
     CDS             1976..2674
                     /locus_tag="ACHA5T_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFM8888022.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2842..5481)
                     /locus_tag="ACHA5T_002486"
     CDS             complement(2842..5481)
                     /locus_tag="ACHA5T_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8888023.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
BASE COUNT         1699 a         1323 c         1106 g         1910 t
ORIGIN      
        1 attattattt atatgacaca cccgattatt caatattgag caatatatcc catgctctta
       61 tctctttctt ttttgaataa aaaatgaggt aggacatctt tctgccctac ctcgtcttta
      121 aataagcttt gatacttatt tttcttcctt ctcttctgtc tcttccagcg tttctgttgt
      181 ttcagttttt acttcaactg tctcagctgg ttcttctgtt gtttcaacag tagtcgttgc
      241 atcatttgtt acgacagtcc ctggtttgac cgttttgatt gctgcacgat cgtactcaag
      301 gaagatccct tcgcagtcta aaacaactgt ccgtttgtca ttgtcgattt ctgaaacaac
      361 tccatgtaat ccgccgatcg ttacgactga atcacctggt ttcatgctat ctaacaagtt
      421 ttgacgttct tgctgttgtt tcttttgcga acgtgacata aaaaaccaca ttgctaatag
      481 cgcgataaac atcacaagca ttggtaatgt tcccattttt ttcacctcac taattctgta
      541 tctacaaaaa acactttagc agaaaaatgc tgataacact agttttttac ttaattttta
      601 aaagattttc ctttagaagc tttttgcgtt ttctttattg aacccgtatt cttcaaaaaa
      661 ggcttctcta aattcaagca aattatcatc catgattgct tgacgtactt gtttcattag
      721 attcaacaag aagtataaat tgtggtaaga agtcaaacga atcccaaatg tttcatcaca
      781 tttgatcaaa tggcggatat aggcacgtgt atagttttta catgtataac aatcacattt
      841 ctcatcaatt ggacggaaat catgggcata tttcgcattt ttcactacta gacggccttg
      901 agaagtcata catgtgccat ttcgagcgat tcgagtaggc aagacacagt caaacatgtc
      961 tactccacgg ataacaccat cgatcaatga atcagcagca ccgacaccca tcaagtaacg
     1021 tggtttgttt tcggggatca acggtgtagt gaattctaat acacgattca tttctgattt
     1081 aggttccccg acagataacc cgccgattga gtaaccaggg aaatccatcc cgactagatc
     1141 tttcgcgctt tgacgacgaa gatcttcaaa ccctgctcct tggatgattc caaataatcc
     1201 ttgacggtct ggattagcat gagctttcaa tcctcgttct gcccatcgag acgtacgttc
     1261 tactgatttt ttcacgtagt cgtaactttc atcgaatggt ggacattcgt caaaactcat
     1321 catgatatca gagcccaatt tattttggat gttgattgct ttttctggtg ataaaaacat
     1381 acgcgaaccg ttcaagtgat ttttgaaatg cacgccttct tcttcgatat tccgcatatc
     1441 acttaatgaa aaaacttgaa agccgcctga atctgttaaa atcggttgat cccagttcat
     1501 aaatttatgc agtccgccag cttcttctac taagtcttca ccagggcgta gccataaatg
     1561 atatgtatta cttaaaataa cgccagcacc catttctttc aactcttctg gggacattgt
     1621 ttttactgta gctaatgttc ctactggcat gaacattggt gtggggaaag ttccgtgtgg
     1681 cgtgatcaat tcccctaaac gcgcgccagt atgtttttct ttcttgatta aacgatagcg
     1741 aatggcaggt tgcgtcattc atttccctac tttctatata aaatatcttg acaactccca
     1801 ccatcataat cgtttcaagt gctttttgca agttccctcg gcttccttct actttattta
     1861 atattctttt ttttatcctt tttttatgaa taacaaaaaa aagaaaaaca ttatgacaga
     1921 aatatttttt gatacatggt atattggtgt tattaaagaa aaggagggaa aaatcatgca
     1981 atcttctgca gaattaaaac gccaagcaaa agagagtcta aaaggacgtt ggggacaagc
     2041 tgttttacta aatctaatcc ctacattgat tggtattgct actattcttc ttattatgat
     2101 tcctattatc ttgtttgcca ccaccatata tgatggatca gcgagcgtct cagaagcgac
     2161 aacttcagga ggcggtagtt caggaggcgg tggcggaatt atttccagca tcattagcgc
     2221 tttgttcatg agcggtattt cttggactta tcttgatatt ttacgtggac aaaaagatca
     2281 gatcgaacca ttcaaagatg catttcgtgg attcaaaggt ttgtttatcg gcggagtgat
     2341 cagcttagct gtgctgatct cgatctttac aacattatgg ggtttgctat tagttattcc
     2401 tggaatcatt aaaagctatt cttattctca aagctatttt atttactacg atatcgtcca
     2461 agaaacagga caaacaccta aagtactgga tacgatcact gcaagtcgaa aattgatgga
     2521 tggttataaa ggacaacttt tctggttaga tctttcattt atcggctggc atatcttagc
     2581 attagcaact gctggtattg gttatctatg gctcagccca tacatttctg caacaaaagc
     2641 agcattttat gaacaattgc caaaagatat ttaaaagaga gtgtgacttg tgaccgaggg
     2701 ggtggcagga gaacaccgtt tatcgagagg cagtaaaaga aacattcagc ccattagcaa
     2761 aaaggaggtg tgacaacatt catgtcacac ctcctttttg tatagttcca gtaaaaagaa
     2821 aatcaactta gaaacagtta atcattcctc ttcttctgct tccggtaatc gatggaactt
     2881 gatcagttca ttcaccgcga ttggtacgat agataaaatc cctaccaaga accagtgatt
     2941 caaactgatg tgtactaggc cgaatagttc ttgcgtaaat ggcagcaaag caacagccgt
     3001 tgtaatagct aatgccagta acgccatttc taccaaagct ttattggtcg ctaaattgac
     3061 tttgaagatc gattggctgc tgcgtacatt gaacacatgc agaatcgatg aataagctaa
     3121 gatcaagaat gcaacagttt gaccgacatg ctgacttgcg tctacccatg ggctgatagt
     3181 atcgacataa ctacctaaat aataaccaaa caaggttacg atggtgaaga cccctgcatt
     3241 tatggcaatt ttttgcaata aacctcgagc aaagatccct tcatttttcg gaatgggtgg
     3301 ctgatccatg atgccgtgtt ccgctggctc tcttcctaaa gcaaatcctg gaagtccatc
     3361 agcaactaca ttgacaaaca gtagttgcac agctgtaaat ggtgctcccc atcctaacag
     3421 catcgcaatc aaaacaataa agatctccga aatattacag cttagcaaaa agttgatagc
     3481 tttacgaata ttctgataaa ccgaacgtcc ttgactgaca gcgtctacga tcgtcgcaaa
     3541 gttgtcatcc gtcaaaatca tatctgcggc actttgcgcc acatctgttc ctgtgatccc
     3601 catggcacac ccaacgttac ttgctttcaa cgctggtgca tcattcacac catccccagt
     3661 catcgctacg actgcacccg tacgttgcca agatttgacg atccggatct tgtcttctgg
     3721 cgttacccga gcatagacag agagagcttt gacacgcgca tctaattctt cgtctgataa
     3781 ttgatgcagt tctgatcctg aaagtgcttc tgacggatca ttcaagatac ctaattcttt
     3841 cgctatggca ctggcagtca ccacatgatc tccagtgatc atgactgttt tgatgccagc
     3901 ctttttcgct cgcttgattg ctcctttact ttctggacgt ggtggatcga tcattccgat
     3961 cagaccaatc aaacgtaagt ctttttccag tgcttcagaa gaaatatctt gcggttcttc
     4021 gtcataagta gcataaccaa cagcaatcac tctcagtgcc cgtttaccaa agcgatcatt
     4081 aacgatcgcc gcttgttcta catcgcctga actaaagcgc ggagcaagaa catcgaatgc
     4141 tcctttggtg attgagatgt atttttcgcc catttggtga actgttgtca tcatttttcg
     4201 ttcagaatca aaaggaagct cgtttactcg tggatatttt tcttctaatt ctgcctttgt
     4261 atgataattc tcttcaacag cccttacgat ggctacttct gttggattac ctttgataac
     4321 aaggtcgtca tcttcttgct cgaccgtcac atctgtacat agtgcagcca ttttcaatat
     4381 ttccatcgcg ctgttcgtca ttgaatcttc gatattcgtt acttcatctt ctttgctcca
     4441 tacccggcgg acgcgcattt cattttgtgt cagcgttcct gttttgtctg agcagataac
     4501 attagcggtt cctagcgttt caactgctgg caactgtcgg atgatcgcat gtttcctcgc
     4561 catcttctgc acaccgtatg caagtgtcaa cgtaacaata acagtcaagg tttccggtac
     4621 agctgcaaca gccaaggaaa tcgaagtcat gaacatctca aggagtggtt ctccttgcag
     4681 ttcaccaatc acaaatacta aagccgcagc cgctaaagcg atcatactga ttcgtttgcc
     4741 tagctgattc aatcggacct gtaaaggtgt tttttgcata gtattttcgt tcaaaagatc
     4801 cgcgatctta cccatttctg tagccatgcc aatggcagtg acgatcgctt tcccacgacc
     4861 tgcagcaacc gtacatcctt taaataccat attaagttgg tcacctaatg actcatcttc
     4921 ttcagcaata aatgctgcat ctttttcaac tgcttcactt tctccagtca acgccgattc
     4981 ttctacacgt aattgtgatg cttcgatcaa acgggcatcg gcagaaatag catcaccgga
     5041 ctctaaaaca agaatatcac ccactaccag ttgctctgac tcaacagtgg tcactttgcc
     5101 gtctcgtatg acagttgccg tttgtttatt catatcttgc aaggattcca gtgctttttc
     5161 tgcgtttcct tcttggacaa tcgccaatac cgagttgatc acaacgatag cgatgatcag
     5221 cagtccttca aataaatccc catgttctgt agcaaatgcg gtataaaagg aaatcaccgc
     5281 tgctacaaga aggatgatcg ttgtaaaatc ggacaagctt cgaaagaatt ttttgatcat
     5341 cgattctttc ggcgcctcat cgaatttatt caatccctgc ttttttcgcc gttcatccac
     5401 ttcttgtgaa gacaacccag tatctggatc tgcttctgtt accttcttta cttcttctat
     5461 tgtctgcttg tattcttcca tcctattcct cctttaaaat cttcgtacaa aaaaaggtac
     5521 ttattttact ataacatcct ttttttattt acttcaaata aagggcttat cgattatttg
     5581 ttaagaaagc gtaaaattta aagtgcttat atttgcaaac gctaccaaag gatgctatta
     5641 tatagatgta aggaaggagg ataccatcaa atagacagaa gaatttctcg atagaacagt
     5701 gtaagcaaac aaagttgtta gaagtacagg tttcacccag acatgcgtgt agagtacaaa
     5761 cctataaata gtgaatgcgt atggatgttt ggatcacgaa gtatccgaaa caagaagtaa
     5821 tgcgatacgt aacaattgaa taacgtaaac gtctgaaaat tgaataggaa caagagatgt
     5881 ttcaaatgtt gaagatgtaa cgaaaacaaa atttttgttt aacatgaaga aattcaacac
     5941 cgtgctttgc atgtatgatg gaaagttaca atcatttctg tttatttgaa tccttccgag
     6001 gtacaagaaa gccgagctta tcgagagctc ggcttttt
//
LOCUS       DAYYGO010000135         6007 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000135 DAYYGO010000000
VERSION     DAYYGO010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.138"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(219..704)
                     /locus_tag="ACHA5T_002487"
     CDS             complement(219..704)
                     /locus_tag="ACHA5T_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888024.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(843..1058)
                     /locus_tag="ACHA5T_002488"
     CDS             complement(843..1058)
                     /locus_tag="ACHA5T_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFM8888025.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            1282..2082
                     /locus_tag="ACHA5T_002489"
     CDS             1282..2082
                     /locus_tag="ACHA5T_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8888026.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            2101..3969
                     /locus_tag="ACHA5T_002490"
     CDS             2101..3969
                     /locus_tag="ACHA5T_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFM8888027.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            3962..4453
                     /locus_tag="ACHA5T_002491"
     CDS             3962..4453
                     /locus_tag="ACHA5T_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFM8888028.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            4441..4995
                     /locus_tag="ACHA5T_002492"
     CDS             4441..4995
                     /locus_tag="ACHA5T_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFM8888029.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            5013..>6007
                     /locus_tag="ACHA5T_002493"
     CDS             5013..>6007
                     /locus_tag="ACHA5T_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFM8888030.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2108 a          957 c         1210 g         1732 t
ORIGIN      
        1 cgacaataaa tttcattaac tcagttgacc gaaaatgaag tagataccaa aaatttatca
       61 agagagcaag tagagaagtg agtatattca taagagaaat agctgtaacg aattattagg
      121 ttaatgcaaa tgcttattta tactccgtat aactttctca gaaaaaatga taccaataat
      181 gacttgctag tactgtaaat ccgtataata tcatctaatc aaaatggtgt tccatttgca
      241 tctgtcatcg taatcgatcc ttcgggtgac actcgataaa aaccaacgct tccagatcct
      301 ccttgggaac ggatttgttt gctggaagct gtgaacaaat aatctcgatc aattttttga
      361 taaaagctaa taacaatatc gtcactttgt aattcggaac ggtgggcaat aagaaataga
      421 agtgctttgt cagagttttc taatggaaga gaaggtacag tgctttcttg catttcagat
      481 gttgaagaaa tgctaaacga ctgaatactt gattggctat gttgcgaatc tacattacca
      541 gtagaagtat tagtaatgga agtagtagaa gtacttttag ttgaaccaac ctcagttgtt
      601 gaaatggctg ctgaagattc tttaacagaa ttctttgttt tatatccaca ggcagaaaaa
      661 agtattacag ttcccaacaa gcctgcgtaa atccctttat gcatgatagc tcctcctaaa
      721 atagattatc tttattcagt atatcactta aacaaagaaa agcagatggt ttaaaatgaa
      781 aaaaccttcc caataaaggg aaggttcatg aagataataa cttttttaat aattatagct
      841 ttctaatatc tttcatcaac ttttctagta atcattggga aaaatttcaa gcagtagatc
      901 gcacaaatac ctacgacgat ataaatgatc ttcgcaaaaa tagttgttga accaccagca
      961 atcatagcta ctaaatcaag ttcgaacaag cctaccaaca gccagttcaa ccctcccaca
     1021 atcaataaag ttaatgcaat agcatctaaa gttttcatta gaattctcct ttcttaaatg
     1081 ataaaacaat tatatatcaa aaagagaaac attgcttata taacgctcag gaattttagc
     1141 aatctatgca aaaagatgaa aatttttttt gctcttccat tagtacacat tttttagctg
     1201 aagctgtaaa tttcatgtct taattttttt tagtgagctt ttatactgga tttgtaagaa
     1261 aaaagaagga gaggatcaca tatgtcggat tggttggata ttgcaggaaa agtagtaatt
     1321 gtaactggag gttcctcagg aattggaaga agtatcgtcg aaaatttgtt gaagcagaat
     1381 gcacaagtag cgaattttga tgtaactgaa tgccgtatac agcatgaaaa cctactatct
     1441 ttaaaagtcg atgtgtcttc taaaacggac atagaagaag ggatatataa agtaatgaaa
     1501 catttcaaga cgatcgatgg tctagtcaat aatgcaggaa taaatattcc ttctcttcta
     1561 atagatagaa atcatccaaa aagcaagtat gaattatcag aacaagtttt tgataagatg
     1621 atcgctgtca atcaaaaaag tgtatatttg atgagccaag cagttggaag aatcttggtg
     1681 caaaagggat caggagtgat cgtgaatctt tcttctgaat caggattgga aggatcagaa
     1741 ggacagagct gttatgctgc tacaaaagca gcgatgaaca gttttactcg ttcttgggca
     1801 aaagagttag gaaagaggaa tgttcgtgtg gtaggtgttg caccagggat actggaagaa
     1861 accggtttga gaacacaaga atatgaagaa gctctttctt atacgagagg tataagcgtc
     1921 gaacagctga gaaacggtta cagtcgaaca agtacgatcc ctctaggaag gagtggtaag
     1981 ctgcaagaag tggcggatct agtctgctat tatctttcag aaagatcaag ttatatcact
     2041 ggagtcacga ctaatatttc aggaggtaaa acaagaggat aaaggatagg ggggacgcac
     2101 gtgacgctag taaacagatg gtaccaatta ttgaaattac tagtcactca caagaaagtt
     2161 tcaatccagg aagtgcaaaa agaaatcaaa accagtaatc aaaccataaa aaaagatatt
     2221 ggggagttga atgaagaaat tgccggtatt gcaaaaatca tccaaaaaaa caaacatttt
     2281 gaacttgaaa tttatgattt tgattttttt gatgagatca tgcaaggaaa attgaaaaca
     2341 gccagtgatt tcaattcggc atccaaaaga atcgcttatt tactaaatag gctgatcgaa
     2401 acagaggatt tcttacgtat ggatgatttg tctgaagaag tcggtgtcag cagaggaaca
     2461 gtggtcaagg atctaaagac attaaaagag atgatcaatg attttgatgt aaagattaca
     2521 ggcacaccaa ataaaggact aagaataata ggagatgagc tagaattacg actactttta
     2581 ttttattttg tttctcccta cttttcagag acgaaaacag aacagtttct agaagaaaaa
     2641 caagcaatca aacattttca gaagaagacg aatgctagta aacaagaaat caccctttta
     2701 acgaaagtta tcggtatttc tttgaataga atctctagta actatctgtt gaagaagccc
     2761 atcaatcgtt acagtgggtg ttttgaatat atccaagaat tcaatgaact gatcaatcag
     2821 ttggaagaaa tctatgaact aactttgagc caatatgaaa aagattttat cagttttcct
     2881 ttgaacatca tcaacacaag tataacgatg ataaggaaag ctaactctga tttgcgctta
     2941 tatttcgacc ccatgatgat gcgtattcga cgattagatc tcattgatct ggatgagtcc
     3001 ttcttatatc aagagatgaa acagcacctc tctttattga taaatcgggt aatttttcgt
     3061 tatcgattga ctgatttatt ttatggtgag atagaaaaaa agtatccttt ttcttaccaa
     3121 atcgctagcg aatgcataca agcattagaa gctatcttgc aacataagat ctctaaagta
     3181 gagatcagct acctaacgtt gtatttggag ttagcatttc gatatcaaaa agagcatttt
     3241 tcctctaaaa aagtagccat tgttcatacg acaggaaaag gaacagcatt aatgatccaa
     3301 agacaaatca aaaggatatt ggggagggat atacaaataa cacatgtttc agaagaacaa
     3361 tatgaaaaag tagatctaaa caaatatttc gctgttttta caacgattcc gttaaaaaat
     3421 gtaaatgaag agacaccggt gatccatttg actaatcttt ttgatgactc ttggttaagg
     3481 gcacaatggc aaaaagcaaa aaaaaagcga ttagccgggt ttcattctac tgtttttttc
     3541 tatcaaatat ggaaagaaaa aaagacgtat caagaatatt taaacaaatt agtcgctcct
     3601 ttgatcagag gctattatgt ggatgaagaa tttgaaaaaa ggatcttgaa aagagaatat
     3661 cagcaaagca catttgttaa caaagaattt gcttttcctc acacaaccaa ccagctatct
     3721 caacacattg ttttatcagt agggctaata actgatcaag gtttagcaga aacaggagga
     3781 aatggagtta tttttttatt tgccatacct gaagaaatga cggctgaagt tgagaatgaa
     3841 ttatttgagc tatacgatat cctatttgaa ctcataaaaa tgggaataga tgagtttatt
     3901 gaattagcac cgacacaaga atcattttta aaactaatca aaagtaaagg agagagactt
     3961 catgaatgat atgtatctat tcatagtttt cgcagcttca gcttatgtgc tgcaagcagt
     4021 tatcagttgg aagcaactaa agcatttcaa tcaaacatat agcgaactgc gaagaaaagg
     4081 aaaagtagcg attggtaaac gacctgggaa aatccgttca ggcacgattg ttatgctagc
     4141 tatagacgat catgatcaag ttttagaagt cagaaaaatg caaggggtta catctttggc
     4201 aaaatttcat tcattacctg tctatgcggg tgaagatatc cattacatcg atcgttacca
     4261 tccctcagtt cgagcggaag atcgtttgac acaagcagct ttagaaaatg ccagagaagt
     4321 atacttacga atagaggcag gaaatgaaaa aagtgaacaa ccttcattgc cttttattgc
     4381 tatgctcaat aattttcgga tatggaaaag tacgatcaaa gaaaaaagga gtgacaaacg
     4441 atggatatca taacaaaatt tgctgaggga tttatgaggc tgttccaaac aggtgcagaa
     4501 acattcataa gctggatgac aggaatcgta ccagttgtct tgatgctgat ggttgcaatg
     4561 aatacgctga ttgctttttt aggtgaagac cgagtaacga aagtagccaa attatcagcg
     4621 aaaaatccgc ttactcgtta ccttgtgtta ccgttcttat ctgcttttat gttgggaaat
     4681 cctatgtcat ttacaatggc tcgtttcctt ccagaatact acaaaccaag ctattatgct
     4741 gctcaagctc aattttgtca tacgagcaat ggtgtattcc cccatattaa tcctggagaa
     4801 ttgtttgtat ggatggggat tgctcaaggg atcgaaacct tagggctaaa ttcgatggag
     4861 ctggctatcc gatacttgct tgtgggactg ctgatgaact ttattggtgg ttggataact
     4921 gattttacta ctggatttgt atgcagacag caaggaatcg ttcttagcaa aaaagtagag
     4981 ctttcagttg attagagaaa ggagagtttt aaatgtatgg tgatattaag atcgtaagag
     5041 gaaacggcgg gtatggaggt cctttgatta ttaggccgac aaaagataaa cacaagttca
     5101 tctatgtcac aggtggaggt gagaggccaa tgatcgtaga taaaatagaa gaactgacag
     5161 gtatggagtc tatcaatggg ttcaaaacat caatacctga tagtgaaatc gcgcttgcaa
     5221 tcgttgactg tggagggact cttagatgtg gaatttatcc taaaaaaggg ataccaacca
     5281 tcaatatcgt ttccacagga aaaagtggcc cattagcaca atatatcact gaagatattt
     5341 atgtttcagc agtagggtta gaacaaatac agccactaat ggaaactgat gataacaaag
     5401 aaacagtaaa tactgcatct ccagataaaa agtacaaata tgatacttct aaaaagataa
     5461 ccgagcaaaa agcagagcag caaaactgga tagcaaaaat tggaatgggc gcgggaaaag
     5521 tagttgctac attcaatcaa tcagcaagag aagcagtaga tacgatgatc cggacagtga
     5581 tcccttttat ggctttcgtt tcccttttga ttggtgtgat acaaggttct gggatcggta
     5641 atgtatttgc taacattatg gcacctttag caggaaatgc agttggatta acaatcattg
     5701 gctttatttg ttcactgcca tttttatctc ctttgcttgg tccaggggca gttattagcc
     5761 aagtgattgg aacattgatc ggtgtagaga ttgggaaagg aaatattcct ccccaattag
     5821 cattaccggc actatttgct atcaatactc aaaatgcatg tgattttatt ccagtcggtc
     5881 taggtttgga agaagcaaaa gctgagacag tagaagtagg tgtaccatca gttctttatt
     5941 caagatttct taacggcatc ccacgagtta tcattgcttg ggtagcaagt tttggattat
     6001 atagctg
//
LOCUS       DAYYGO010000136         5919 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000136 DAYYGO010000000
VERSION     DAYYGO010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.139"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(145..603)
                     /locus_tag="ACHA5T_002494"
     CDS             complement(145..603)
                     /locus_tag="ACHA5T_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFM8888031.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(616..1947)
                     /locus_tag="ACHA5T_002495"
     CDS             complement(616..1947)
                     /locus_tag="ACHA5T_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFM8888032.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1958..3400)
                     /locus_tag="ACHA5T_002496"
     CDS             complement(1958..3400)
                     /locus_tag="ACHA5T_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8888033.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3387..4151)
                     /locus_tag="ACHA5T_002497"
     CDS             complement(3387..4151)
                     /locus_tag="ACHA5T_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFM8888034.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4255..5130)
                     /locus_tag="ACHA5T_002498"
     CDS             complement(4255..5130)
                     /locus_tag="ACHA5T_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8888035.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5385..5469)
                     /locus_tag="ACHA5T_002499"
     tRNA            complement(5385..5469)
                     /locus_tag="ACHA5T_002499"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5433..5435),aa:Leu,seq:caa)
     gene            complement(5487..5558)
                     /locus_tag="ACHA5T_002500"
     tRNA            complement(5487..5558)
                     /locus_tag="ACHA5T_002500"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5524..5526),aa:Gln,seq:ttg)
     gene            complement(5568..5640)
                     /locus_tag="ACHA5T_002501"
     tRNA            complement(5568..5640)
                     /locus_tag="ACHA5T_002501"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5605..5607),aa:His,seq:gtg)
     gene            complement(5702..5786)
                     /locus_tag="ACHA5T_002502"
     tRNA            complement(5702..5786)
                     /locus_tag="ACHA5T_002502"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5750..5752),aa:Leu,seq:caa)
     gene            complement(5807..5877)
                     /locus_tag="ACHA5T_002503"
     tRNA            complement(5807..5877)
                     /locus_tag="ACHA5T_002503"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5843..5845),aa:Cys,seq:gca)
BASE COUNT         1814 a         1258 c          900 g         1947 t
ORIGIN      
        1 tttctaaatc ataggagcaa caaaaaaatt cgataattaa caaaaatcaa aatgattaaa
       61 tctagtgatt gaaacatatc atttcaaaaa aattttgtaa attcacccat aaatatcacc
      121 ctatttaact tttttcatcg tacactaaaa ttcaaaacaa ctatctttaa tctttacttc
      181 ttggttttgt tttaataaat ttccttcaaa aatgcctgga gaaactacaa tgatcaccgt
      241 atctttttgt gcttgctcca acttcttcaa atctataata gctaacagtg tcccttcttt
      301 tacctcctgg ttactttcaa cccgtatctg aaacggactt ccttcgagtt caactgtatc
      361 taaacccata tggatcagta tttttctgcc agattcactt tcgatcgtta tggcatgtcg
      421 ggtaggaaat atattatcga ttattccttt aactggcgca tggatatatc catcatgatt
      481 cgttatagcg tatccaactc ctatcatttt tgaagaaaag acctcgtctt ttactgctgt
      541 taatggaata atttgaccac tagctacagc ttttagaatg tttcttgttt taaagaaatg
      601 cactttttat cacctctact ttaatgctgg ccaatatttt ttgtttactt tgatcatttc
      661 atctagtatt ttttttgcga ctctggcatt tggtacggtt tgattcaatg taaatgcttg
      721 taaagctttt tgataagaac cttcaaagta agcatctact agtaacttct ctactgcaac
      781 ttgtgcctcc atcaatcctt tatgaaaatc actgattggt tttcttagtg taattggctc
      841 cacaccagtt gcaccaacat atgccggtat ttcaacaact gcatctgacc gtagattcgg
      901 aatcgcccct tgattaggaa cgattagcat aaagcgacga cgatcatcat tcaacaagga
      961 aatagctata tcgacgatat attgtccatg ctccccaaaa ttaaagttca atacttcttc
     1021 cacaataccg cggcgtattt tatcagccat ttctttggta tttttctctc gtccatccat
     1081 gaccatatta gcacgtgtat actctttgtc agtatgctca acgaccatat caggatataa
     1141 gtaatactct aagtaattat tcgggatatt acttggaaaa tttttcgtga tgactgacat
     1201 catttgaaag gttttttgcc aagttttatc tcctatatta aaatcggcga cttgcatttc
     1261 ttttgttatt agtttttcaa taatctctgg cataatttct cgttgcaatt ctttatcata
     1321 aatcgatgta taccagccaa agtggtttaa accataatac gtaacgatcc aattcttacg
     1381 atcataaccg taattcactg caatcgtttc ttcaattgaa atagtcatat cacaggcatt
     1441 aatcattttt acattcggat atttacgacg aacagcttca gaaacgatcg actctggatt
     1501 tgtataattc aaaatccatg ctttgggggc atagtcttgt atatgatcga caatttccaa
     1561 taaacctttg attgagcgca tcccataagc aaatcctccg agtccacacg tttcttgtcc
     1621 tactaatcca tgtttaagtg gaatcttttc gtccttttct cgcatctcca atcctccaac
     1681 acgaatttgt gagaaaacaa aatcgcatcc tgtaaaagca agctttggat catccgttgc
     1741 acgaatgcga atatttgaaa aaccttttct tttcagcata aatttaacaa tcaagtacat
     1801 atccatattt ctttcttcat caatatcata taaacgtatt tcagataatg gcaaccgttt
     1861 ttcattgttc aaaatggctt gaacgattcc tggtgtatac gtactcccgc ctccagctat
     1921 agtgataatc tgttttttca tcatcatctt ttcctttcta ttcttctaat aatttattga
     1981 attctgccat aacataagtc acttctaagc cgatgataat ttgataattt ttttcagttg
     2041 gtttaatgat cccgcttatc cctaattgct tgattcgctg ttcctcgacc atggtgggat
     2101 ccactacctc tacccgtagg cgtgtattac aactatagac atccaggata ttggcttttc
     2161 ctccaaacgc ttgtagcatt tttgttgcca tatattcata atttttactt gctaacccta
     2221 actctgtttc ttctaaggtt acttcattgc caaactctgc cttagcttca cgtcctaatg
     2281 tctgaatgtt tctcttattg atcaaataat aaaaagtaac gtaatacaag gcaaaaaaga
     2341 aaattccgat tggaagaatc aaaatagcat tggtagcgat tcgaaaattg atgagataat
     2401 caacaataca tgctccgaat gtaaaaccaa ttcgtatatt gaataagtaa cagacaatcc
     2461 ctgctaaacc ggcaaaaagt gcatggatca cgtacaacat aggagaaaga aacatgaaac
     2521 taaattcaac aggctcagtg atatttgcaa taaatgaagt tcctgcaccg cttcccatga
     2581 ttcctttgac catctcttta ttttctttat aagctgcttt atagatagct agacatatag
     2641 ctggtatacc aaacatcatt actacaaaaa aaactgataa aaacagtcca gcctgaggat
     2701 ctccgttgat aaagcgtgtc atctctcctc tgaaaacttc cttgctagga gatgtatagc
     2761 ttcctaaatc gtaataaata tacgtattca acacatggtg cagtccaaag gggatcagca
     2821 aacgattcaa aaacataaat atccccacac ctaatgctcc catatcaacg agcagttttg
     2881 caaaattatc aatcccaatt tggatgagcg gccagataaa gcccaaaaaa acagctgcga
     2941 ttgtttgtag aatcatcacc atagtcaatg ggaatttttc gcccgcaaaa taagaaaaaa
     3001 ctactggtaa tttttgttct ttaaagcgat tatacgtcca tgccgctact aaaccggaaa
     3061 taattccacc aaaaatcccc atattcactt ctgggtccat aatttctaag ccttttttca
     3121 aggtagcaat cgctaaaaaa ccagtaagtg caggtattcc tttgtcctta tttttgacaa
     3181 aaccgattgc aactcctata gcaaataaga tatccaagtt tccaaaaacg gcatttccag
     3241 ctgcaccaat tactgggatg ttcagcatat cgtcagctga taaacgcatg atcaatcccg
     3301 ctaatggcat cgctgcaact ggaagtagca tcgctcttcc aagcttctgc acatttgatt
     3361 taacattcat ttttattcct cctctattaa atatcctcat atgttactaa ctcaatcaaa
     3421 ggatcatttt tcactttttg agcaaatgct gaatgaatta aaaattcgac ttggtgggta
     3481 cgaggataca caaaacttga accatttaac aattcctgat ccaaatacgc tgcgtgacac
     3541 atgacttcga tcgttttatc ttctctcaat atcatgagta aacttgccaa atattcttcc
     3601 acactcgtat tgatttgtcg ttttttttca tcagtttctc ctacgacatc gaaacaaggc
     3661 tcaaaataat cgacatgacg aacttcatct tttcgttcaa agttgctccg aaccggcaaa
     3721 tcatattttt ttgctaatgt aacaactacc tcctgatttt catccaaagt atgagtatga
     3781 tgatgactgt ctaaatgagt tggtatgatc cccgcatgat aaattttttg aatctgagca
     3841 ttccattcct gatacagttg atctttgtca atgttaaatg gatgctgata ataagataag
     3901 gatctgaact ctccgctttc gataagagta tcgacagttt tgaggagtgg tttgccacac
     3961 gtcaatgtta aatggactcc tacacctagc ctaggcattt cctttcttag tttcacagca
     4021 tgctcgaaac ccggcatatt tgccatcaat gtagttgatg tcaaaattcc tcgctgataa
     4081 gcatccataa tcccataatt cacaccatgg ctatagccaa aatcatccga attaaaaatc
     4141 acttttccca tttcattctc cttctttttt ctactaaaat aggcgcaacc aaataatcat
     4201 agtccattta ctatgtgtca tttggttgcg cctatagata gttacgccct tattttaata
     4261 gattcttgct agatgaattg ccaaatagat gatttcattt tcttgaatgg caagttgaaa
     4321 ttgctgttcg atgatatctt taatctttag tgctgtacaa tacgctttgc tatgtttaaa
     4381 tgtttcagta aaaaaaccaa tattttctac tggcgtttct tcctgataaa tcaagcgttt
     4441 aaccaggaat ttgatatgaa ccattaagcg atgataagtc agtgattctt tatcataaac
     4501 gtttgaatag gatttttcta gatgggacaa aatcgtggtc gtcatttcta ctatttttaa
     4561 gctctttaaa ctattaggct ctcctagctc agcattaatg atatgcatag tgatgaatcc
     4621 tacttcattt gaccctaact cggtttgata tcgatgattg attcgctcga ccacttctac
     4681 tgctatatca tattcatttg gataaaggaa tctcatttct ggcaacataa ttgctgtgac
     4741 atctctatct tctctttttc gttgaatagc aaaataaata tgatctgtca atgtaatata
     4801 gatatttgaa tctaatggag aggagatcct agctgttatc agttcaatca cttcttccgc
     4861 aaaatccata aattcgattg gaatctgcga gactatctca ctgaatcgtt tgatcttttc
     4921 tttagaatct aatacaaata atttgtcagc atcctcaaca tcaaatggct tactcacttt
     4981 agcagaaaag ccaatccctt tccccatcgc aattttttct attccatcgt catcgatcaa
     5041 tacagcatta tgattcagct ttttcatcac atacaccgtt aattcacctc ctctatatga
     5101 taaaacaaaa caacttgcag actccttcat acatattttt gtctccaaac aatttttaaa
     5161 gacaaaaaac gacctaagaa agaacccttt cttaggtcgt gcctaattga atagtagcac
     5221 cctacataat ttacaaaaat agtttaacac ataataaaag cgttatcaat atctttttta
     5281 ctcgttttat gataatactt gtatcgaatt gatttggagg taattacata aaaacaaaaa
     5341 aaatatagca actctctata agaaagttgc tatattgata attataccgg cggccggggt
     5401 cgaaccggca cgtcctcaac ggacactgga ttttgagtcc agcgcgtctg ccaattccgc
     5461 cacgccggca aatttaaaaa taataactgg ggtagctgga ttcgaaccaa cgcatgaggg
     5521 agtcaaagtc ccttgcctta ccgcttggct ataccccaag aataaagggc gaatgatggg
     5581 aatcgaaccc acgaatgccg gagccacaat ccggtgcgtt aaccacttcg ccacattcgc
     5641 catcataaaa gatgttattt aatttctcaa taaaaaacta agatgaagac atcttagtca
     5701 ataccggcgg ccggggtcga accggcacgc cctcaacggg cactggattt tgagtccagc
     5761 gcgtctgcca attccgccac gccggcaagt tgatttatta gtactaaggc ggtaaccgga
     5821 tttgaaccgg tgatgaaggt tttgcagacc tctgccttac cacttggcta taccgccatt
     5881 atactattat caatctataa aataaagaat aaattatag
//
LOCUS       DAYYGO010000137         5914 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000137 DAYYGO010000000
VERSION     DAYYGO010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5914
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.140"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..629)
                     /locus_tag="ACHA5T_002504"
     CDS             complement(<1..629)
                     /locus_tag="ACHA5T_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8888036.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRL"
     gene            777..2012
                     /locus_tag="ACHA5T_002505"
     CDS             777..2012
                     /locus_tag="ACHA5T_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFM8888037.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEEIETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPALSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHHLTPYSQRQTIEQDSAI
                     DSGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNESKPEI
                     TPGEKPDVDPEEKPDVTPEPDTDSGNQTVPETNPDTDNETENPEKPEVAPEEKPDVTP
                     EPDTDSGNQTVPETNPDTDNETENPEKPEVDPEEKPDVTPEPDTDARDQGIPEKINKK
                     TIQEDGKKESKKSNLAILKINEEQLNKKSRIFDSAQSAETLKSSKDTTFAVLKRKINS
                     YQNPESHKTKLYCGAESFY"
     gene            1985..2065
                     /locus_tag="ACHA5T_002506"
     CDS             1985..2065
                     /locus_tag="ACHA5T_002506"
                     /inference="COORDINATES: protein motif:HMM:TIGR01167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888038.1"
                     /translation="MLWSGIILLSIATMLSAKRFKQNRSL"
     gene            complement(2191..3270)
                     /locus_tag="ACHA5T_002507"
     CDS             complement(2191..3270)
                     /locus_tag="ACHA5T_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8888039.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(3373..3813)
                     /locus_tag="ACHA5T_002508"
     CDS             complement(3373..3813)
                     /locus_tag="ACHA5T_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8888040.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(3870..4280)
                     /locus_tag="ACHA5T_002509"
     CDS             complement(3870..4280)
                     /locus_tag="ACHA5T_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFM8888041.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(4460..4894)
                     /locus_tag="ACHA5T_002510"
     CDS             complement(4460..4894)
                     /locus_tag="ACHA5T_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8888042.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(4994..5842)
                     /locus_tag="ACHA5T_002511"
     CDS             complement(4994..5842)
                     /locus_tag="ACHA5T_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8888043.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
BASE COUNT         2158 a         1110 c          983 g         1663 t
ORIGIN      
        1 agtcggtcaa gtacttcttt tttaaaaaaa gtattcatca taaaagccac tacttgcgga
       61 tcttcatcat aaaattctgt agcagtactt gaaaagatgt tccaataaat atctaatgct
      121 gcatattgaa tttgtacctc ttttccagaa aaagactttc tgccattatt agaaaaggag
      181 atagacaaac cgtacaatgc taaaaatgca ttaatctttt tcattttcgt atacaaataa
      241 gaggcactat agtttgtttg ctgacttaaa ctgataatcg aatttgtctg acagctgaac
      301 aaagaagtca agatctgata actgactgat tcgttgcagt atcttccaaa tagacgataa
      361 tagcagtaag ccggatcaat tccttcaaaa ttcaacttta aaaaaggttt ttgcttatca
      421 atatgcagtt ctgttagatt gagacgctca aagtcttgtt ctaattcttt gatgtatagt
      481 aaaaaatttg attcagacat atggaaatga tctgctaaat attgtgtagt aataaagaaa
      541 cgatgttcag ataggaaaag aaaaatatct aattttgtat tgcttttttt cttcaagaga
      601 aacgttttaa tcgcttcttg agtttgcatg aaagaggctc ctttttactt aaattgttaa
      661 aacttgaaaa tattactgtt tttttcataa atttagacaa aaaaacagta aacatatagg
      721 tagaatctct actatcttat ttcttgcata caaaaaaatc aaggagtgag agagttatga
      781 gatattcaac catcttaata tcaacatttg cagccttaag cttatcgctt actataaatc
      841 agacagtcca cacagaggag atagaaacag ccaaatggac agaaaccaag tgggccaacg
      901 tcaatcgaat tacctttcag gatgaatttt catccttcaa ttcaggagtc gcaaaattaa
      961 gaaaaggaca aacagcgacc atcaaagcaa aaattgacta ttcaggagac ttagcaccta
     1021 tactacaatc agctgttaca tttgagaatg ttgatcccgc tttgtcgatt ggctatgacg
     1081 agaacacttt gactatccaa cacaagcaag cgatatttac cttcaccgtt acattaaatc
     1141 aggacttgtt aaaaccagcg ctttttactg tcaaggtatc agatggacta ccgaacgatc
     1201 atcatcattt aacgccgtat agtcagcggc agacaattga gcaagattca gctatcgact
     1261 caggaggaga tcttgttgaa gaaccaacag ataaaccaga aaatgaaaat aagccagaag
     1321 taccgcctac agaaaatcca gacggagaac agaaaccgga aatagaacct ggagaagaac
     1381 ccgatacaga aactcaacca gagccagaca atgaatcaaa acctgaaata acccctggag
     1441 aaaaaccaga tgtagaccca gaagaaaaac cagatgtgac tccagaacca gatacggatt
     1501 ctggaaacca aacagtaccg gaaacaaatc cagacacaga taatgaaact gaaaatccag
     1561 aaaaaccaga ggtagcccca gaagaaaaac cagatgtaac tccagaacca gatacggatt
     1621 ctggaaacca aacagtaccg gaaacaaatc cagacacaga taatgaaact gaaaatccag
     1681 aaaaaccaga ggtagatcca gaagaaaaac cagatgtgac tccagaacca gatacggacg
     1741 caagagacca aggaatacca gaaaaaataa acaaaaaaac aattcaagag gatggaaaaa
     1801 aagagagtaa aaaatcaaat ctagcaattc taaaaataaa tgaagagcag ctgaataaaa
     1861 aatcacgaat atttgatagc gcccaatcag ctgaaacctt aaaaagtagc aaggatacaa
     1921 cctttgctgt cctgaaacga aaaataaaca gctaccaaaa tccggagagt cacaaaacaa
     1981 agttatactg tggagcggaa tcattctatt aagtatagca acaatgcttt ctgcaaaaag
     2041 atttaaacaa aacagatctc tctaacattt gaataatgtc tgtgaacgca tttttcaaat
     2101 aaaaagaaat atccagaaaa tgaccccaaa ataccagatt gaaatctgat atttggggtc
     2161 gttcatactt tatctttctt catatttatt ttaagggata tctgtttctt ttggaagatg
     2221 gttcgggatc tttttcactt tcaatacatc taaaaagaag gtaaagatcg gaatgccgac
     2281 aatcaagccc caaacgccaa agaaccgttc gctaacaagt aagaccacaa atgtataaaa
     2341 aatcggcagt tctgttttgc tggacatcaa cttcggattc agcacataag actcaattag
     2401 atggacaatc aaaatcgtaa tcaggatata aacaacatct cggatgcctc cttgggaata
     2461 agctatgaaa gatagaggaa tacaagaaat gataacacct gctactggga tcagacttaa
     2521 aatgaaaatc atgatcgcta aactcagcag ctgatgaaag ccaaatacag ccaaggcgat
     2581 tgtagtgagc aacgtattta aaagagcgac aacaaattga gcttctaata ccaaaccaaa
     2641 tgtattgaca aacttatcgg caaaatagta aatatcttga aagaaccaag aaaattcact
     2701 ttttaaaaac aatcttgaga atacaattgt ttttttcttt tcaatcataa aaaagaaact
     2761 taagatgaat gacatagcaa acgccactgc catttttcct atatcctgta aatagccaag
     2821 cgcaatcgaa gcgccatttt gaagctgttc aaaaaaatcg tttcgctcaa tataagtatg
     2881 gatatattgt aaaacaggat ctgcatcttt tggctgatgc tcgtaaaaat taataacgga
     2941 ctggatcatg tcaaatgttt gatgaataag catcgggata tactttgtga atgccagata
     3001 gataaggtaa acgatcagtg tataaatcat gatagctaaa acacctgtag ggattcggac
     3061 aaatcgctga atgatttgaa caagatgaat catcaaataa gtaaagataa atgtgagcaa
     3121 aatggttgta atcatacttc taactaagaa aagaacaaaa acgacaaata ataaaacagt
     3181 gagacggcgt attttttcat tcgccaaaaa acgctgataa acggtcaaat cgacaacctc
     3241 caaataacaa aaaataaaca cgttattcat tatatgatgg aatatgaaaa taagaaagag
     3301 caaaaagaaa aaccgctaaa tgacgttatc ttatgagttg gttttcctca taaaatcgtt
     3361 catctagcag tttcagccct ttttctggtc ttgatgaaaa ttatctagca tttttcgggt
     3421 cagatgttgc agtgtatcca attcttcctg ttcaagtgga agataagact tgatcttttg
     3481 tggaatacct tctgcttgtt tttgcaacgc agcaccttga gcagtcagcg taacatttac
     3541 gattctttca tcacccgttt cacgctgacg attaatataa tttttttctg ctaacttttt
     3601 tagtaaggga gtcagcgtac cagaatccag acacaaatca tttcctagct ctttcactga
     3661 agtctttttc ttttcccata aaactaacat cacaagatac tgggtatagg taagattcag
     3721 cggcttgagg aggggagtgt agtgtctaat gatttcttta gaggcggcat ataaaggaaa
     3781 acatacttga ttaactaaat atagatcttc catttttcca cctccgctgt tttactatag
     3841 tgtatcatag cggatttaca caaaaaagtc tattcactaa ctgctttcag agtaacttct
     3901 atatttccag ctgtagcttt tgaatacgga cacactttat gcgctgcctc taataattct
     3961 tgcgtttctt ccagagaaag accttcgata gatccttcaa tctctacacc taagacaaag
     4021 ctcgtttcac tttctgaata aagggaaact tgtgcactga cagtagaagc cccagagata
     4081 tttttttgct gtttgatcaa ttctaatgca ctgttaaaac atgcactgaa accagctgca
     4141 aacaattgtt cagggttggt tgcattttcg acgcgtttac caggagctgc aatattcaat
     4201 tcgaatgtac gatcgggatt atgcacttct ccagagcgtc cgcctgtgtt gatcattttc
     4261 gtagaataca tttttttcat aaaattacca cctttaatta tattttatac aattgaattt
     4321 tacacaatat aattgtggaa agcaaagaaa tggattggca taagataaat aagagaaagc
     4381 acatgaaaca gacgcgcttt ctcgaatagc aaacatatac aaaaaatatc agagacaaaa
     4441 aataatctca tcataaacat tacatatatt tctcaaaaag ttgttttctt tcttcaaaaa
     4501 ttacttcacg atggacctga tgaagcatcc ataaataacc aaaaggatct ctgaacattg
     4561 agttagtcac accaaagtcc atcatttctg tcatttcttg aatctctaca cagccatttc
     4621 gtaatgcttt attatgtgtg tcctcgatat cagagacgat gatattgaac cagatcgtat
     4681 ttggatgttc agcagtagga gcagtcaaac caagagacgt gttttcatcc agcatatgga
     4741 atctcgtacc atacaaggta aaaatgactt cattattgcc tctagggtaa tctgttgcct
     4801 caatcagttg tatttcatca aagattgttt gatacagttc tagtgctttc aaactatcat
     4861 ttaatacaaa atcgatttcc gttcctttat gcataaagat ctcctctcct tttcagtcga
     4921 acagttacta gattctttta ttataccagc taaagaaaac gcattcaaaa aaatagcgga
     4981 tttacttacc aactcattcg attggatgat aagtaaatcc gcgtcccatt acttctgcta
     5041 cgctgctgac aatgacaaag gcacttggat cgatttcttc tatcaatggt ttcattcggg
     5101 aaaattcatg atcagtcaca acgatcaata aaacctcttt tgactttcga ttatacccgc
     5161 cttttgcctc tagcagggta aggccacgtc caatctcagc acttaagcga aggcggatgt
     5221 cttccaaata atgttcactc atgaccataa cagatttctt tcgattgata cctgtttcga
     5281 tataagtcat gacaattgaa gtgattgcaa tagataaaat agcataaaga aactcttcta
     5341 ttccaaaaat aaacagatta agagaaacaa cgaatgcgtc tgtaaaaaat aagccaatcg
     5401 aagtattcaa gtggaaatat tttttaaaaa tgaggggagg gatcgtcgta ccgccgctag
     5461 aagagttgtt tttatataaa atcgcaactc ctaaagcaaa aatcgcactt ccaaaaatga
     5521 cagacaacaa ccgatctgaa gtaatcattg tttctggaac tattgcaatg attaaaggaa
     5581 aaaccaagct tccgaaaagt actttaaaaa agggtttttt acctaaaaat agaagagcta
     5641 gtaccaacat aacgatattc aacactaaaa taactatagc tcggtcaaag cctaaagcag
     5701 cttccagtaa tattcctaga ccgctgacac ctccagcagc aatatgatga ggccctaaaa
     5761 acatattgat agcgactcct aatatgaaga gggcaaaaat aatcttaatg tatgtccata
     5821 taaattgtct cgtttctttc atttattcac ttccttaatt cattaataat actcttatct
     5881 tattataact tacttttcac cttaagttaa cttt
//
LOCUS       DAYYGO010000138         5810 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000138 DAYYGO010000000
VERSION     DAYYGO010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.141"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(49..2220)
                     /gene="copB"
                     /locus_tag="ACHA5T_002512"
     CDS             complement(49..2220)
                     /gene="copB"
                     /locus_tag="ACHA5T_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFM8888044.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(2240..4426)
                     /locus_tag="ACHA5T_002513"
     CDS             complement(2240..4426)
                     /locus_tag="ACHA5T_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8888045.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(4426..4635)
                     /gene="copZ"
                     /locus_tag="ACHA5T_002514"
     CDS             complement(4426..4635)
                     /gene="copZ"
                     /locus_tag="ACHA5T_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFM8888046.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(4648..5088)
                     /locus_tag="ACHA5T_002515"
     CDS             complement(4648..5088)
                     /locus_tag="ACHA5T_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFM8888047.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(5162..5701)
                     /locus_tag="ACHA5T_002516"
     CDS             complement(5162..5701)
                     /locus_tag="ACHA5T_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFM8888048.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         1504 a         1305 c         1015 g         1986 t
ORIGIN      
        1 tcgaaaaaaa ggttgtgaca cgttttttgt cacaaccttt tttcattatt atctgatttt
       61 caatgtcaaa gcatttagag ctacaactac cgtacttaac gacatcaaga cagcaccaac
      121 tgctggactt aatatgatac caattggcgc aagaattccg gctgctagcg gaattgcaag
      181 aatattgtaa cctgcccccc accagaggtt ctggatcatt ttctttcgcg tttgcttggc
      241 tagatcaaga aaatgcaaaa tatcttttgg atcgctgttt gtcaatacga catccgcaga
      301 atcgattgcg atatctgtcc ctgcaccgat tgccatcccg attgaagcac gggctaaact
      361 tggcgcatca ttgatcccat cacccaccat gattactttt ttcccttggt caaggtattt
      421 ctggacaaca gcttctttat catctggtaa taaaccgcca taatattctt cgatccctaa
      481 ataatctgct actgcttggg cagcgttttt attgtcacca gtaagcatga caggagtgat
      541 ttttctttct ttcaatgttc ggataaattc ttttgcttct gtttttacta cgtcacctaa
      601 agcaacgatt gcagctagat gattttctaa aatcaggaag ctgatggtat ttccttgttc
      661 ttggtaaggt ttcaataatg cttgttctac gtttaatccg agacgctcaa cttctttttc
      721 attaactatt ttcacttgtt gttttttgac tgttgcctct agcccgactc ctgaaagatt
      781 ttttagattt tcagcttgat aaggcttgat ttcatgttct ttcaaataat tcataatgcc
      841 aacagccaat ggatgattgg cattcgcttc caaggctcct agatattgaa ggatttcatt
      901 tttattgaac gcatcatcaa gtacttcgac acccgttaca gtaaattttc cttctgttag
      961 tgttcctgtt ttgtccagca tgatcacatc tagatcattg gcttgctcca gtgcattgcg
     1021 gtttttcaaa agtaatccgt ttttagctgc tattgatgtg gaacgagcaa caactaatgg
     1081 gatagctaaa cctagagcat gaggacaggc gatgataaag actgttacca tacgttctaa
     1141 tgcattagga agatctgtca agaacaacca ggcgatgaat gccaggatac ccacaattaa
     1201 tgcgacataa aacagccatt ttgccacttt gtcagataat gactctagct tggatttttc
     1261 tccttgtgct tttcgcacca tttccataac cttcgctaga taaccatttt caccagttcc
     1321 agttacttcg atttcgatcg ttccatcccc attgatagaa ccgccaatca ctttgtcatt
     1381 ggcttgtttt ttgattcctc ttgattcgcc agtcacagct gattcgtcaa caattgtttc
     1441 acctttcaag attacaccat ctgttggcat tttatcacca gaccggacga tcaaacgatc
     1501 accttcgttt acttctttca atgaaactgt ctcttctttt ccatctttcg tcaagcgttt
     1561 cactgattct ggcagtaatt ctgctagttt ttgtaaagca ttgctggcat tcgacacagc
     1621 attcatttca atccagtgac ctagtagcat aataacgatc aaagtagcca attcccagaa
     1681 gaaatccatg acgtgtgtat gtggattgag cagatttgcg ataaacgaat agacactata
     1741 aatatatgca actgtaatcc ccatcgcgat cagtgtcatc atagcaggac ttttttgttt
     1801 caattccatc ttagctccac ttaaaaatgg ctgaccgcca tagataaata gtatagttgc
     1861 taagatcaat acgagccaat cagaaccagg aaacgttact tgaaatggaa attccattcc
     1921 catcattggt gaaaaaagaa tgattgggat agctaggata agcgatagcc aaaatttttg
     1981 tttgaaattc cccatgtgca tggaatgatc catgccgccg tgttcgtgat ccatgtgcat
     2041 cgaatggtcc atttgtttgc tgtttgctat atctccatgg ttatgtccca tgtgcatgga
     2101 atgatccatc tgactatggt catgtttcat ctccatctgt ttttcatgag attctgtact
     2161 ttctttgttt actacttctt ttttatgctc atgttttttc tccatatcgt tcccattcat
     2221 ttttactcca tcccttctct tactttttca tgtgtcgatt taagcacagg gagttcaaca
     2281 atacactgac ggaactaaac gccatcgctc ctcctgcaat gattggattc aaaaagccta
     2341 gtgcggcaaa aggaatacca atcgtattat agacaaaagc ccagaaaaga ttttgtttga
     2401 tttttctcaa tgtcaaccta gacaactcga ttgtttgtgc aatggatgcc aaactgctat
     2461 tcatcagcgt cacatcggct gtttccatcg caatgtccgt tccacttccc attgccatac
     2521 cgacatcagc taatgctaag gccggtgcat cattgattcc atcgcctgcc ataccgactt
     2581 ttttgccatc tttctgaagt ttttctacgt aggctgcttt atcctctgga agtacttcag
     2641 caaagatatg ctttggatca ataccaactt gtttaccaat cgtttcggct gccagtttgt
     2701 tgtctcctgt cagcataaaa acatctaatc ctttattctg gagctgcttg attgcttgtt
     2761 tgacttctag tttgatttga tcagccacgg caatcaaccc gatcacttgc ttttcatcag
     2821 ctaaaaacat gactgttttt ccctgctgtt ctaattctaa cgcgtgttct tggtattcat
     2881 caaatgatag gttcaattca attaatcgtt tacgtgtacc cgcaaaatag cgtactccgt
     2941 taatcgtacc gctgattcca gcacctgggt gggctgtaaa atcaatcata tcgtatgttg
     3001 ctgcttcttg cagttttccg tattccacga ttgctttacc tagtggatgt tcagaagctt
     3061 gttctaaagt ataaaaaata gataggactt ctttagaacc gactagatct gtaactttgg
     3121 gctttccttc tgtaatcgtt ccagttttat ccaaaacaat gctgtctaag tgagcagctg
     3181 cttctaatgc ttctccgcct ttaattaaaa ttccatttcg tgcaccaaca cccgttccta
     3241 ccataatagc tgttggagtc gctaaaccta atgcacatgg acacgcaatg actaatacag
     3301 agacactatg gatcaatgcg agttgccaat caccagtgat caaacctgta gctatgagcg
     3361 tgataaacgc gatgactaat acgatcggaa caaaaatccc tgagatccga tctgcgattt
     3421 tttggattgg ggctttgctc ccttgagcat cttccaccat ttgaatgatt tgcgctaata
     3481 ctgtctgctt gccgacttgt gtaacttcca tgtgaagtag accattcgta ttgattgtcc
     3541 cgccaaagag cgtgtcatcc ggattctttt ctacaggaag attttcacca gtaagcatac
     3601 tttcatctat cgcactgctt cctgaaataa ttcgaccatc tgctggaatc tgctcaccag
     3661 gacggatgac tagttgatca ccaacgacta cttcttcaat cgctaacgtt tcttctttcc
     3721 cgtttcttat cacttgggcg gtttttgttt gaagagacat caattgtttg attgcattac
     3781 ctgttttcgt ttttgctgta tgttccaaat atttgccgag caaaatcagc gtgatgatca
     3841 tactgctgct ttcaaaataa agatcttggg ggtgtccacg gaaaaatccg ttgtaaatgc
     3901 tcaatgcaaa ggcagccgat gttccaattg ctaccaatac atccatattt ggtgctttcg
     3961 tcttgattgc atggtacgca cctttataaa aacgagcacc gacataaaat tggacaggag
     4021 cagacagcac taactggacg attggaaaat gaaaaaagtg aacaatcgcc gcatggcttc
     4081 ctaacatcat agcaatcatt gacaacatca acggcaaagt caaaaccgta ctcaatataa
     4141 gatcgaatag catttttttc aaatacgctt gcttttcttc tgcaattttt tgtttgtggg
     4201 cttcatcgta caaaattgca ccatagccga tgttttctac gctttggatt aatttttcag
     4261 tggtcgtccc ttcatatttg acacttgctt tttcagtggc caagttgact gtggcattga
     4321 ccacaccagg ttgtccgttc agctcttttt cgatcctagc agaacaattt gcacaagtca
     4381 tacctgtaat gacgaacgta tccattttgg tttctgctgt tgccatcaaa tcacctctgc
     4441 tttataaccc aatccattga ttgcttgaca aatcttttcg gattgaactt ttgtttcatc
     4501 aaacttgacc gtaccgtttg cttttttcaa atttactttc actttttgca cgccgtcaag
     4561 tgcgcttact gtttcttcta ctcttgctgc acaatgattg cagctcatac cttcgattga
     4621 aaattgttgt ttcatttttt cttcctccta ttgatgttgt ttacattcac attgacctgg
     4681 gatacagtta cattcgatcg tatctactgg ttgcttttgt tccagttgtt tcaaaatctc
     4741 attgatatct tcttgagtca aagtggcttc ttctactaga tccgcaatcg tctctccaat
     4801 cctttttgca cagatatggg aaaaaagatt ttccgtcgca ctgcgaactg tttccatctc
     4861 cgagacagct ggataataaa tgaatttttt tccttcttgt tctgtccaga tcacttcttt
     4921 ttttaccaaa cgacccagca aagttttgat cgttgctggt ttccaatcca tcgaatcagc
     4981 taagatctgc gtgatttctt gggctgtcgt ctgtccaagt gtccaaataa cacgcatgat
     5041 ctcccactct gaatcactga tctttactgg cgttttgact ttttccattt atttcacacc
     5101 tttcgtttac aaatgtaatc attatcttat gaatacagat tacacttgta aacaaaaact
     5161 gtcaattatt tttgctctct attttcaatt tagttaaaaa ttcttgtttc tctcgtcttg
     5221 ttttaaggat gatcagttta tttcttgcat tagctacttg gatcagctgc tgtattttcg
     5281 gttcattgtt ttcttcaaaa tgccagataa aagataaaaa ttctaagtat tccctgttta
     5341 gtttctccga aaatgattca ggcatatctg gccgagattt tttgtttatc tttcttttta
     5401 gagaacgcca aactactcgg tagatggcga gataacgcgg cactttaagc caaatgatct
     5461 tatctgcttc tttaatcctt tcctctaatg tagaagtata atttccttca acgatccatg
     5521 acggattaga taccataaac aattgctgtt cttgcaaaaa ccactttttt gcctgaatac
     5581 tgtagtctgt tgtatgccat aaaaggtcta gcggcagtag cggaaagtcg gtgatttctt
     5641 tgatcttcct tgcgagggtc gattttcccg acccactttg tccaatcacg atgatcttca
     5701 ttcttctccc tcacttctct ttggaatttg gttcgatcat accacttata acgccgcaaa
     5761 aaaacaaggg actgtgacaa aagtcgtgtc aaagtccctt gtttcgaata
//
LOCUS       DAYYGO010000139         5772 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000139 DAYYGO010000000
VERSION     DAYYGO010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.142"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(228..1010)
                     /locus_tag="ACHA5T_002517"
     CDS             complement(228..1010)
                     /locus_tag="ACHA5T_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888049.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1075..1911)
                     /locus_tag="ACHA5T_002518"
     CDS             complement(1075..1911)
                     /locus_tag="ACHA5T_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8888050.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(1929..3338)
                     /locus_tag="ACHA5T_002519"
     CDS             complement(1929..3338)
                     /locus_tag="ACHA5T_002519"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8888051.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3352..5175)
                     /locus_tag="ACHA5T_002520"
     CDS             complement(3352..5175)
                     /locus_tag="ACHA5T_002520"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM8888052.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
BASE COUNT         1765 a         1257 c          945 g         1805 t
ORIGIN      
        1 tcttaaaaat tttttcacta aaatcactac ctgaaacagt ctaaaacact tatatatcaa
       61 cgtttaatga aactatctcc gtttcatttc agatacaaat aaatatagtc gggctccaaa
      121 tccgaaaaaa aataccccca gtagcttatc ctaagctact ggagatgtat aagtacggtc
      181 atataacaac ggggtagggt aaataaatga aaaagatgga agaatcatta ttcaataact
      241 tttttaaata cttcgttgta atcagttacc tgtccgttct taaataccac agaataatct
      301 tttttcccat tttcaagtaa ccgaagtgga atgtctttac caacttgctc tgtcactgca
      361 ttactgatac ctgttagatt agctaataaa tctgataaag attgagcatc tgtagaaatc
      421 tctgtatcag tatttatcgt gattccttta tcttcattta caagcttcca agtaggtggt
      481 atagctgtgt atgtttcgat gaatgaatca attctttcat ctatcgtctt cccagtatct
      541 acttctgtct cattactctt agatgaatcg attctttcat ccatcgtctg ctccgtaact
      601 gcttctgtct cattattctc accggaaact ctcttcacac ccaaacttat atattgtcca
      661 tctttagtgt gcatagttaa aattcccacg cttttactaa taaattcact ataaccagtt
      721 ctttcaatag agacttcaat gtatttcaca ctatcatact caaaagaaaa atagacatgt
      781 aaaagcattt tgttgtttac atctacataa taatcctttg actctacttg agaccttcct
      841 atacggaata cagctttact gtctgatatc tctataccag catccttatt attaatctgc
      901 caatctccaa gtagctcttc tggcaagcta gtagtttctt gtgaacactt taccacttca
      961 ctggtagagg tcgaaacatt cacctccgca ctgttagaag aattttccaa ggaatcagga
     1021 gccttagcac aaccaacaag tctttatata ttgtgcagcc gatattttct tcattcagta
     1081 cacccttgaa atatgcagtg ttagataaag cagttcgtcc tgatttgctg aggcacccat
     1141 ctcttcttga acgtactcct tgattttcaa ggccgcccga tactctttcg gaaaacgctg
     1201 cttcactgaa gccagcaaaa aatcatcctt ctccggcaac tcttccttct taatcattcg
     1261 ggcaataaaa aactgtaaat gcgtcacaaa tcttaagtaa agcatcgaat ctttatcaac
     1321 ctttttttcg ggaaacagat atcctagcat agccaagata tcctttagtg ctcttaccga
     1381 gagcatggtt tcttccatat ttgcttgttc actttgggca tttacaatgt gaaacgcaat
     1441 gtttccagcc tcctcttctg gcaactgaac attcattttt ttgttgatca attggatagc
     1501 atagcagccg atctcaaatt cttttgggta atacttttga atttcaatca gcaggcgatt
     1561 ttgaaaaaca atgccttttt gactgcgttc aatggcatgc ttcaagtgat caaccaaggt
     1621 aaaaaataga cttttattca gcttaaagtc aaacttggcg ttggcataat cgataattcg
     1681 cttgacgcaa ataattagat catccggagc agcctcaaac agtttaatgt aatccttttt
     1741 tagagcatca gaaagtgaat ctaatttaaa tactttttgg attttgtcag ccggaaccaa
     1801 atcgcctttc tttagttgaa atccaatccc tttccccatc aaaattactt ctttgccttg
     1861 cttatctaag caaagaatta aactgttgtt taacgtttta attactctca ttagattcac
     1921 cgttctctct aagctaagcc atttcttgcg attacttttt gataccaaaa gaagctttct
     1981 ttttttattc gttttaattc tttaagattc tcatcggttc ggtcaacata tataaaacca
     2041 taacgcttat tgaagccttg atgggtacta acaacatcaa tcgctgacca aggtaaataa
     2101 ccaaaaacat ctacaccatc tgttaaagct gcttgaatag ccagaatatg tttggctaga
     2161 taatcaattc gatcctggtc attaattcgt ctatcttcct caactatgtc gggtgcccca
     2221 tagccatttt cagtaattaa aataggtaaa tcatagcgat cgtacaattt cctcaatgtt
     2281 aatcgtagtc cagtagggtc aactacccag ccatatggcg ttttatctac ataaggattt
     2341 tcagctgccc ggtagacacc ttcttctccc aacatgatct gttgatcccc cgctctagcg
     2401 gaaacatctg atccgtcatt tttgctggca gcgatggtgg cagttgaata ataattaatc
     2461 gcaataaaat cgggtttacc ggaggataaa attgcctgat cctctgcctc aattttagga
     2521 tataatccac gatcattcaa atagttttca aacaaataat tgtattttcc ccgaactgct
     2581 acatctaaga aagaccagcc tctcaatgtt tcccaattat gggctgcaat tgcatcttct
     2641 ggactggagg tagcttgata catagccgtt aaattgattg ctggaccgat ttttccttta
     2701 agattcaact gatgatacaa gtgaattact ctagcttgag ctaataaaac atggtgatta
     2761 atttgaaaga gttctttttt tgaaggcagc tctccacttg gtggcaaacc aatcgctcca
     2821 ggatgaagga tcatcgtgtt ttgctcatta attgtcagcc aatagttcac cttgtcacca
     2881 tactcagtaa gcaatacctg tacatagtcc acaaaataat cgatagttgc tcgatttaac
     2941 caaccgccct gctcctctag aaaataaggt aaatcgaaat ggtacatggt gacaatcggt
     3001 tcgattccct gattcaggca ttcatcaatt acttgatgat aaaaagaaat tcccttttga
     3061 ttggttttat tattttcaat atccataatt cgcgtccaag cgatggaaaa gcgaaaagct
     3121 tttaagccta attctttaaa cagctgtata tcttctttat agtgatggta aaaatccgag
     3181 gcaacttcaa aattagctac cccttctgta tgctgatact tgtcaataat agacggttgt
     3241 ttcccatcct cctgataggc accctctgcc tggtaggcac taatggaggc accccataaa
     3301 aacttctctg gaaatgctga aagctgtttg tattgcacaa aaatcctctc cttaactaat
     3361 ttgcatcaat ggttcgtgtt cctttaagct tccgcttgct tcactaattc ctgcgtaatt
     3421 ttcactgttg gtcactacca caatcactgt atcatcgtag ccagcgtttc taatggcagc
     3481 gaaatcggcg ttgattaata attgtcctgc tgacacctga tctcctgctt ttacaaatac
     3541 ttcaaagggt tcccctttta aattcacggt gtcgatcccc acatggatca ataattcaat
     3601 accttcttta gaacgtagac caatcgcatg tttagattct aaaatagtaa tgacttctcc
     3661 ctcaacaggt gaataaatgc tgttttcttc tggatgaatg gctacgccct ttcccaatag
     3721 tcccttagaa aagacagcat cgttcacttc agaaatagga cacacggttc cttttaccgg
     3781 attttcaaca atgactgctt cggctattgc cgtttgctct gattcatcag aagtatcgtc
     3841 aaatccaatt agataagcaa tgataaaggt tgcaacaaag gcgattacac acgtaataat
     3901 tgcgtgaaca atattcattg gattttctcc aataaaggct ggtaaggctg ctaagccagg
     3961 ggaaacaaag gcatatctga ctaaaccgct caagccagca tacaaaccag caattgcacc
     4021 accaatcatg gaagcaacta atggtttttt cagcttcaag gttactccat agagtgaagg
     4081 ctcagtaatt cccattaaag cagtgatccc agtggacaca gctagctgct tcaactcttt
     4141 gtttttagtt ttcgcggcta cagcaaaggt agcacctgat tgagcaatat tagaagccag
     4201 cattcctggc ccattgatca tttcagaacc cgtactgctc aattgcatag tagcgattgg
     4261 cgtcattgcc caagctgtac ccgtaaccac caaaaatggc tgcaaaccac ccatcaaaaa
     4321 tggaattaac caagctgctt tgccgttaat ccattcagcc cccattgcta taagatcgtt
     4381 cagcaacgtt ccaaacggtc caatgacaac caacgctaaa ggccccataa ttaaaaatac
     4441 cagcatcggc ttagtaaaga atttaataaa cgaaggcgaa attcgatccg caaagggctc
     4501 tacataggac atgatccaaa cagtaaagat aatcggtaaa actgatcccg cataatctgc
     4561 agctaatacc ggaattccta gaaaagaaac ggcatcgcca gtagaaaaaa gctgtgaaat
     4621 atttggatga agcaaaatgc cagcaacagt aatcgccaaa attgggttga catcaaactt
     4681 tatggctgcc ccataggcta atagtactgg cataaaataa aaggctgcat cagcaatagt
     4741 attcaaaatg atgtaagttt gactactgtc tgatagtaca ccagtcaaag tcaagacggc
     4801 caaaatggct tttaccatcc cggcacctgt tatagctgga atcatcggtg taaaggtggt
     4861 agaaatgaca ctaatcaatc ttgaaataat actttcttga ttgtctgaag aggggccctt
     4921 tttaattgaa gttcccactt ccttttgaat ttccttgaag gctgtttgga cttcattgcc
     4981 aatcaccact tggtattgtc caccgctatc caccacacta attacaccaa gtgttttttc
     5041 taaggctttc gtatcggctt tccttttatc tttgagttca aaacgcagtc tggtggcaca
     5101 atgggttaag ctgaccacat tttcttttcc cccaacctgt tgcaatacat ttcttcctac
     5161 ttctctgtaa tccattattc tctctcctta aagcagtaaa aaagaccaag tcaaaccacg
     5221 ccccaatcaa cattgaagtg ttagtttgtc ttggtcttgc ctgcataatc agtaacatgc
     5281 caataacgtt attttgctgt aatccaataa tagcacatta tgatagcgca atcaacaggg
     5341 aatttaattt tttatctttt cgtttgaccg tatagggtaa tataacagtt agatcacatc
     5401 aactagggta tatctgcgta tacttactgc taaaataagt atatctaata ttaactatac
     5461 cagtaaaacc attcctacat aaccagcggg aaattcccaa aatcgtgaaa gcatcaaaaa
     5521 tgcatataac ggatcacaca acttcaacaa ttctgctctt gggaaaaatt gatgaatttg
     5581 gtgagtaaag gatggaataa acagcattcc aacaatatag gcaacaatag tgatacttgt
     5641 ttttttggta aaaatcagta agcaatagcc aatttcaatc attccgagat ataaaggtat
     5701 cgatagaatg atttgggcta gtattcttaa aaattaacta gcaaatgttt aaactgattt
     5761 atttctaatc ta
//
LOCUS       DAYYGO010000140         5762 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000140 DAYYGO010000000
VERSION     DAYYGO010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5762
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.143"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            47..396
                     /locus_tag="ACHA5T_002521"
                     /pseudo
     CDS             47..396
                     /locus_tag="ACHA5T_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(668..958)
                     /locus_tag="ACHA5T_002522"
     CDS             complement(668..958)
                     /locus_tag="ACHA5T_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888053.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1360..1596
                     /locus_tag="ACHA5T_002523"
     CDS             1360..1596
                     /locus_tag="ACHA5T_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888054.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1603..3555
                     /locus_tag="ACHA5T_002524"
     CDS             1603..3555
                     /locus_tag="ACHA5T_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFM8888055.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3627..4124)
                     /gene="dfrG"
                     /locus_tag="ACHA5T_002525"
     CDS             complement(3627..4124)
                     /gene="dfrG"
                     /locus_tag="ACHA5T_002525"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFM8888056.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(4451..5143)
                     /locus_tag="ACHA5T_002526"
     CDS             complement(4451..5143)
                     /locus_tag="ACHA5T_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM8888057.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            complement(5421..5660)
                     /locus_tag="ACHA5T_002527"
     CDS             complement(5421..5660)
                     /locus_tag="ACHA5T_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8888058.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
BASE COUNT         1863 a         1019 c          931 g         1949 t
ORIGIN      
        1 aagcattact taaagataac ttaaaagtta tcttttaaat acttaattgt taaatacctt
       61 atttgatgca aaagaaatct acgatagtca acaaaaaaat tttcattata tccagaaaaa
      121 tttttcttag ttaaggacct atggattgca gtaatgcaga attcgtcgaa taaactgcca
      181 aagacataca atcatattgc ttttaaaatt gacgaacaag atatagattc gtttgtctca
      241 aaaatccaaa tgttaggttt aactgtcgaa ccaggacgtt ctcgtgtgaa aggcgaggct
      301 tcttcaattt acttttacga ctatgataat cacctcttcg agttacatac cggtactctc
      361 caggaacgtt taaaatccta caattccact acctgagact agtaatgact agtctctttt
      421 tatggcctct gatcccccca ttgagcgaat actaataggg cttcgaattg atctgttcaa
      481 tctttaagca atatccctca aatgagtttg gaaagtttag tgattcatca caaatactta
      541 aatacatgta gttatatata gattgaggtt cttgtgagcg tatgctcgtg tgggttcaaa
      601 tcccatcgtc tgcatccatc tttccaaaac aagcggctag gacatttttg tctcagccgc
      661 ttgttttcta gtttattctt tcatcttgtc ggaccttttt tcttgggtat agacttttct
      721 catattctac cacaattttg ggtttcccta acacatgata ggatctcaca acaaattgct
      781 ttctattatt ataataacct gtaacgacta acttcatccc ttcgtctact tcagctagga
      841 agttcaaact atgggaagca atcaaacaat tgaccccatt taaactgaaa cggatcaacg
      901 gtctggcact aacttgcaaa gtacggatct tatctactgt tccatatttt ttctccataa
      961 ccttcacctc aataccatta tacgaacata cattcccttt gtaaagaaga aactctgttt
     1021 tatctttttg actttttgtc tattaccttt cttaaaatca tattggcact tcctgcgaag
     1081 atgaaggaca tgtactaaaa aaaagaggca gcagtataga tttaagtagt tcagttttgg
     1141 agtacaaaaa gtgaaaatgt aaacctaaat gagtatcaaa ttgcaactga atttgatact
     1201 cgttttttat tgttaaatca acgtttgttg ttaatttaca ccacaaaatg taagttaaaa
     1261 tgtatataaa aatgcgaacg tgaaataatt attgtaggaa tgcaaattgg tgtgttaaaa
     1321 tggttacaat ttactaagca aaaaggggtg cattttccta tgcaatttct tgagccaaaa
     1381 aataagaacg ctaagtctgt ggattgggaa atatcagagc aagtaagggt gatagttaag
     1441 caatacgctg aatatgcaga gcgtactgaa agcgaagcag tggatgaatt cttattgaac
     1501 attttagacg ataagaagtt tattgaatgg attgccaaca agcgaagtaa taagcggatt
     1561 gttgaaaaaa tgggtatcaa agatagagtg ggttgataga caatgaccaa gcttaaaaga
     1621 ttagccacga aggaagatga aattgtagat gttaaaattc cgatttcaaa cgaagaatta
     1681 aaagataggg ctaaacaata tgatttatta actccaaaac gatttgcaac aaggtataac
     1741 aaaatgttgt tcttgccaac gtcttttaaa tggaacggtt ctgaatatcc cattcaatat
     1801 aattactgca tcaatccgtt ttgttgtaat tttgggaaag aacagcacaa attcaaagat
     1861 gtaaaaggga aaccaagtcg atataaaatg actggtagtt ccaaagataa aggtcataaa
     1921 ggtatgtact gcaatgacaa tcctattggc agaggtgtat cgcaaaactg tacagtaacc
     1981 cctttatcta attggtctgt tgttgaagaa attaaacgcc ttatagaaat caatagcatc
     2041 caagatgttg agccagatta tcaatttcat aaagaaggct gctcggaaga agaatcaacc
     2101 ccattcaatg agccgaagca attttacaaa agggggaaaa gtaggggcaa atctcaacgc
     2161 tatcaatgta aggcgtgtaa gaagttcact aatgttctac caaaaagaga agaaaccact
     2221 acttatcacc aacaaaagaa caccattctt ccaatgttcg ctaagatggt tgttggaagg
     2281 gtttcagtca gtcgtacttg tgatatttta ggaattggag ttggaaccta ctatcataag
     2341 ctggagtggc tgtataggcg ttgtttggag tttttagaac gatatgagac acaacccctt
     2401 caaacaaaga aatttaacga gatgtggctt aatacggaca agatgcacta ctacctaaat
     2461 aacgtccgta agaaaggaca aggttcaaag aaatatacgg ggtttgaaga ccttaatatg
     2521 caaacgtata tagttgtatc tgctgaggtc ttatcaagat atgtgttccg ttctgatgtt
     2581 gcctatgatt ggaatatttc aatggatgaa ttaaatgaag atacaagaaa attcaaagaa
     2641 gaccatctca atacttttag tcgtaaaaat gataggttag attggtctta ttatccacaa
     2701 gaaccatccg ctaacgatag cgaaaatagg aatgcttatc ttcacgaatt agggaaaatt
     2761 actaatagga gtagatttgt cgatggttta aatgttgatg ctccttatac tacaactgct
     2821 cactattggt taattaaaca aatggttaat gccgacgaat ggcgaatgat tagtgatgat
     2881 gacttctcta ttagaaacgc cttttataga gtattcacga aagaattaag gttgagcgat
     2941 gcacatcatt ttatttgcca agttaacaag acgaaaagca gaaaacaatg cttaaaagaa
     3001 ttcggacaag caaaagcaga actgctcgat tggggagata tcagaggatt caaaacaaag
     3061 tttctacgga ctttggcatc tcattatcta actgaattac taacaagtca tcaattccac
     3121 gaagaggcta ttagtaaaga tggagaaaga tatcgcaaat atgccgataa ccctatcaaa
     3181 catccattag ccactaagga taaaggattt tattcagttg attgtagaac ggatttatct
     3241 gctctggaac caaacgaaat cgcaaagatg ctattaaatg ttaatgacca ttcgaccaat
     3301 agctttattc aacagataag acgttatata tcatctcttg aaagaccatt aacaacagct
     3361 cgtggtgata agaagagcta tatttacgct aacttcaatc cgaagtatgc tcaatttgcg
     3421 attacaattc taagaactta ctacaacttt tgtcgccctt ttaaatcagc ggataaaaag
     3481 gttttgacac ctgctcaaag attagggatt acagataaac aatttgattg gaaagatata
     3541 atttatttca aatgacaaaa agagcttgga gtaatcccaa gctcttgtgc tttatcttta
     3601 ctgttcttct tcaattaacg cacccgttaa ctcaataagt tttttctttc atatacatga
     3661 aaatagtagt tatacggatt tttatcattc tttatccctt tttgggcaaa tacctcattc
     3721 cattcctcat aattcacttc tggaaaaaaa gtatctcctt cgaattcatg atgtattttt
     3781 gtgatgtaca ttttctcaac ataagggaaa aacaaattat aaatctgttc tcctccgaaa
     3841 ataaaaattt cttcttcgtt tttacataac tcaaaaacat cttctattga atgaacaatt
     3901 tcacaaccat taaaggtaaa ccccttatct ctcgtcagaa taatatttct tctgtcaggt
     3961 aaggctcttc cgattgattc aaggttcttc ctacctaata ttatcggatg tccctttgta
     4021 gtatttttaa catattccca gtccttggga atcctccaag gaatgtcatt ctctttgcca
     4081 atcactctat tcttatccat cgcagcaatc aaagaaactt tcatttataa cacagccttt
     4141 ttaactaatt taccatctaa tttcttattt ctatcaaaaa accttttctc attatcctgc
     4201 ccgttagttg aataagcaat tttttctata agttatatta tcataatacc acaaaatcac
     4261 aaattttcat tcaattatca ttttcaattg cattttataa atcattttgt atgtaatttg
     4321 gtatgtaagt taaaatgaaa aaagtagtcc attttgaagt acattttgtc attttgtact
     4381 ccaaaactga actacttagt atagattact gttgcttctt tactaattta ggtttacttg
     4441 ttaagaaaga ttatttacgc aaatgttctt caacaatttg ctgagattta tcagctgtat
     4501 cttccacttc atcttttgct ttttctaact tttgaatata ttgtttcaag cttgagtttt
     4561 cagcagttgc ttcgtctaat ttcttttgtg tatctgttaa ctgagtttct acatttgttt
     4621 tttcttgtgt cagtgttgcg atttgttgat tcaaaccatt tacagtattt tgtaattctg
     4681 aaagttttgc ttgtgcttct tgtagtcttg ctgtccaatc tgcattcaga tgattgattt
     4741 catcattttt ttggttgatc tcatctagtt tgacattgat ttcattttgt ttgctttgta
     4801 gttgtgcatc cttgctgact aattgacttt ctaagttttg tttttctgct gccgattgat
     4861 ttttttggtt ttctaattca tttacttttg tttggtattc ttggacatct gcctttgaat
     4921 cttgcaatgt ctgttctttt tgtgctagtc gagcggtaag atctgtaatc gcttgctctt
     4981 tatctgtcaa tgtatgatca agctgtgtca gattctcatt gattgtatca atatctgtct
     5041 ctccacccca caaaataatt tcatttgcgt accgcgtgcc tgtgtagcca attagacata
     5101 gaaatgcaac gacaatccca ccagtaataa atttcttttt catttgcata gctccttaat
     5161 ccaatacttt tacatgctaa aagtaagtga tctcaaacac aatgtcaagc gtttttaaat
     5221 tacatatgta atttactcta aattttatat ctaattttca attatccgca taaacactat
     5281 acattcaagc attttgaagt atagaaaaac actcatctta ccacgaattt accacgattg
     5341 aatgagcctt gatatgaccg taaaactact aagggagata gcacgaaact gtgtatctcc
     5401 cgattttttt atacagttga ttacttcact ccgatttcaa atttcaatat tgctttcatc
     5461 atgtctgtat gaatttctcc ttttagttct tcatcaacct gcataatgat tttaccactt
     5521 tcagagtggt aaaaaggacg caagcataat ttagaaatat aaccgtcata aaacttcaaa
     5581 atctctttaa ctgccatttc atctccgctt gacgctttgc aaatcaattc aaaagaggga
     5641 tagccataat ctttactcat ttctttactt cctttcgctc aaaagcattt ttaacttcct
     5701 tagtcctcta gttcttctac tgcaaatagc agaacgtgat acatggtaca gttctgaaat
     5761 tt
//
LOCUS       DAYYGO010000141         5686 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000141 DAYYGO010000000
VERSION     DAYYGO010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5686
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.144"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            122..823
                     /locus_tag="ACHA5T_002528"
     CDS             122..823
                     /locus_tag="ACHA5T_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFM8888059.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            820..1941
                     /locus_tag="ACHA5T_002529"
     CDS             820..1941
                     /locus_tag="ACHA5T_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFM8888060.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            1956..3188
                     /gene="pepT"
                     /locus_tag="ACHA5T_002530"
     CDS             1956..3188
                     /gene="pepT"
                     /locus_tag="ACHA5T_002530"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFM8888061.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            3320..4228
                     /locus_tag="ACHA5T_002531"
     CDS             3320..4228
                     /locus_tag="ACHA5T_002531"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8888062.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            4263..4535
                     /locus_tag="ACHA5T_002532"
     CDS             4263..4535
                     /locus_tag="ACHA5T_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFM8888063.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            4546..5592
                     /locus_tag="ACHA5T_002533"
     CDS             4546..5592
                     /locus_tag="ACHA5T_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFM8888064.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
BASE COUNT         1849 a          976 c         1232 g         1629 t
ORIGIN      
        1 aagaaaatgt tgttttatag aacgaaaagg tatatttttt aaggagatat gggaattttt
       61 ttattcctag tctccttatt ttatttggaa aatgctataa ttatctagaa ggagtgaagc
      121 tatgaaccat ttagatttat ctcagcgctt agccgcagtg ggtgactttg tccctgaatc
      181 cgctcgttta gcagacattg gatcagatca cgcttactta cccgttgcat tgatattaag
      241 aggaaaaatc gaatatgcag ttgctggtga agtcgttcaa ggtccttatg aatcagcacg
      301 taagcaagtc aaaaaagatg gattagaaga ccgtatcacc gtacgccttg caaatggtct
      361 agatgctatc cagccacaag atggtattac agctgtcacg attgcgggta tgggaggttc
      421 actgatccga gatatcctag aagctggcaa gttgaatcaa cgcttaagcg gacaagaacg
      481 tttgatcctt cagccaaaca tcggagagcg tacattacga tcttggctgc aagaaaatca
      541 gtataaaatc caatcagaaa aaatacttga agaaaataaa aaaatatatg aaatcattgt
      601 agccgaaaaa gaacaaaaac cagttactta caatgaaatg gagctgatgt tcgggccatt
      661 tctgcttgaa gagaaaagtg agatcttcaa atcaaaatgg acaagagagc ttcaacaaag
      721 aaaagctatc cgtgaacagc tcaaacaagc agctaatcaa gaggcacgtc aaaaacaaat
      781 cgagcaggaa atccaaatga ttgaggaggt tttaaagcca tgacaatcag cggacgcact
      841 ttcatccaaa agtttgaaag ctattgtcct cagtggctag ctgaagaagg agacccagtc
      901 ggactgcata tcggaacatt ggataagcca atccaacgag taatgatgac attagacgtt
      961 cgtccagaag tggtcgaaga agcaatcaag aaaaaaatcg atttgctgat tgctaaacat
     1021 cctccaatct ttcgtcctgt aaaacggctg gttactgatc agccacaaga aaaaatgtat
     1081 gcagatttgt tgaaacatga tattgccgtc tatgcagcac acacgaacat ggatattatt
     1141 tgggatggac tgaatgactg gttttgtgaa cttctcggta ttgaagtgga gagttattta
     1201 gtaaagactc atgagattca ttataaaaaa ctggccgttt atgtacctgt ggatcatgca
     1261 cagaaaatgc gtgaagtatt agctgcagct ggtgctggta cacaaggtga ttataccggt
     1321 acaagtttta cctcgattgg acacggtcga tttactccgg aagctggtgc acaaccagcg
     1381 attgggaaag tagggaagac agaacaagtt caagaggcaa aagttgaggt tattttgccg
     1441 gaaactatcg aaaaacaagt gattcaagcc atgcggtcag ctcatcctta tgaggaacct
     1501 gcttatgatc ttttcgctat agatgaacca gtcgaaatgt ttgggttagg acgagtaggt
     1561 gaattgccgc aagaaatttc tatagaggca tttgtagaac aagtaaaaga agcgttccaa
     1621 ctagatggac tgagaatagt ccagccaaaa aatgcaaaat cctctgtgaa gcgcattgcg
     1681 atttgcggtg gaagcggtga aaaattttat ccgcaagcaa tcgcccaaag agcagatgtc
     1741 tatatcacgg gagatatcta ttatcatacg gctcatgata tgcaaagcgc tgggttgatt
     1801 gcaattgatc ccggtcatta tattgaatcg ttatgcaagc aacgatttat cgagaaattt
     1861 gaatcttgga aacaagaaga aaattgggat attaactttt tcgtttctga aacaaataca
     1921 aacccatttc aatttaatta aaaggagaga atactatgta cgaaaatctt ttgccacgtt
     1981 ttttacgata tgtcaaaaca gaaacacgct ccgatgcaac aagcaaaacg actccatcga
     2041 ctcagacaca agttgctttt gcgcaagaat tgaaaaaaga attagaagag ttaggcttag
     2101 ctgatgtttt ctataatgaa gataacggat tcgttatggg aacattacca agcaatgttg
     2161 atcatgaagt gcgttccatt ggatttattt cccacatgga tacagcagat tttaatgcag
     2221 taggagtcaa tccacaaatc attgaaaact atgatggcga gtctacgatc gtactagatg
     2281 cggaagaacg ctatacattg aatacaaaag attttccaaa tttgaaaaat tatgaaggcc
     2341 acacattgat cacaacagat ggcagtacat tactgggagc agatgacaaa tcagggattg
     2401 cggaaatcat gactgcaatg gagatcttga tcaataatcc ttctatccct cacggtacca
     2461 tcaaagtggc ttttggtcct gatgaagaaa tcggtgtcgg agcggataaa ttcgatgttg
     2521 agcaattcga tgtagatttt gcttatacag tcgatggtgg tcctttaggt gagttgcagt
     2581 atgaaacgtt tagtgctgct caagcaaata ttacgatcca agggaaaaat gtacatccag
     2641 ggacggcaaa agatacaatg atcaatgctc tacagttagc aatcgatttt cataatcagc
     2701 ttccagccga tgaagttcca gaaaaaacgg aaggatacga aggtttcttc catttgatgg
     2761 cattggatgg gaatcctgaa gaagcttcta tgagttatat cattcgcgat catgatcgtg
     2821 aaaaattcga agcacgcaaa gcacaaatca cacatatcca agaagagttg aatcagcgat
     2881 ttgatcaaga acgaatcaaa gtagatatgt atgatcaata ttacaacatg aaagaaatca
     2941 ttgaaaaaga tatgagcatc gtggaattag cgaaacaagc aatgatcgag cttggtatct
     3001 ctccagtgat cgaaccagtt cgtggaggaa cagatggttc aaaaatctct tacatgggta
     3061 ttccgacgcc aaatctattt gccggaggag aaaatatgca cggacgattc gaatttgttt
     3121 ctttacaagt aatggaaaaa gcaacagacg tgattgtaaa aattgcagaa ttgaacacaa
     3181 agagttaaaa tcgaaaaaga acagcttatg acaaggcgtt cgtcccaatg atggaatgtc
     3241 atctgtctaa ggctaaaggg caggacaatg gaattggttg tcccgccctc ttttttgaag
     3301 agtaaatagg aggtaaagaa tggaaaagct gctagttttt tataacaaaa gttcagggaa
     3361 agatgagggt gagaaattag cacgctggtt tcaagattat gtaaaagaaa agcagccaga
     3421 attatatgta gaactagctg aaactggccc atcgattgat gatgcggaat tagtcaagaa
     3481 agcagaagcc atagaagctg atactttagt aattataggt ggagacggca cgattcatca
     3541 tatcgtccaa gcgttccaaa aaacattatc tcaataccat gtcggattac ttccaggtgg
     3601 aactgtcaac aaccttgcac gtgttcttga gattcccctc aaaaaagaag aagctgctga
     3661 cgtgatcctg gaaggcaatg tacggaaaat agactatggt caagtcaatg atgaagtcat
     3721 tatctctaca ctgacaatcg gaatcttagc agatacagca gtttggatca gtcaagaaga
     3781 aaaacagcgt tacggctcgt ggatctttat caagaagttt tttaagttat tgctgaagaa
     3841 gaaaaaatat catttggatt tagaaacaga cgaggagcat tggaaaggaa aatcccaact
     3901 gttgacagtc acgatgtcta actccgtggg cggttttaca aattttgacg actcagcgac
     3961 cctagatgat ggaaaatttc atgtaacgat cgtaccttcc ttagatattt tccgatttat
     4021 tttttatttg cctagaatca tgagaggcaa attctatacg atccccggca ttacttattt
     4081 cacagccaaa cgcatgaaaa tccaagcgca ggaaaaatct gtgggcactc gaacggatgg
     4141 agacacgaca gatgatcttc cagttgaatt atctgttgta tcaaaaggat taacagttta
     4201 ttcaccaaag aaagaagaaa aagcttagaa atagtagtta acagaaagtt ggaagaagag
     4261 taatggatgt attgttacac aatcctttgt ggttttacct tttcattgtg aatttttact
     4321 tattttgtct gatggggtat gataaacatc aggctagaac tagcggctgg cgtgttcctg
     4381 agaataattt gatatttgtc agcctgttag gcggtggttt gggaggattg atctctcgaa
     4441 aagttttcca tcataaaact cggaaaaaga agtttactat aggattcgct gcaggtgtac
     4501 ttgttgattt gattctcatt tattttttcc attaaggagc aggagatgtc gtctaaagga
     4561 acgaaaacta aaatcattct gaatctgctt ttgctgattg tgatttttgc cattatttat
     4621 tatttaatca atcaatcatt tgcagatatc tttgccgaat tgctgtctac cagtgttgtt
     4681 gttttggcag cggctgttat attagggaca gtttaccaaa tcgctgaagg gcgctcaatc
     4741 aaagaaattg ctgaaccttt cgcgccgaat tttacaacga aagatgggtt cttgacctct
     4801 agctatattg cgttttaccg aatcgtttcg tttggtacag gaacgctgat ctcggagatt
     4861 tatttttata acaaaaaagg aatgaagcta tcccagggag caggggtgac agcactccat
     4921 atgattatgt acaaactggc agtgatcact tatgcattag ctggcttggt catccaattc
     4981 tctctttttt attctaatgg tccatcaatg atctggttta tcatcgctgg tatccttttg
     5041 acgatcgtga ttgttgcctt tttattggcg atgtccgtca gcttgaatgt tcaaatattt
     5101 tttgtcagct tatcgaataa gttatttaaa agtgagcgtt tgcggaaatg ggtggataca
     5161 tgcaatcagc agatctactc tttaagggaa acggtccaaa ccattatcca agacaggact
     5221 gcgctattgc ggatttatct atggaatatg ttaaaacttg tattttggta tgtacttcct
     5281 tatttgattc tgattgaaaa ccatcctaaa atagattttc tactgaccat ttcgtttatc
     5341 agctttgcag tagtgctttc aggggttatt cctacgcctg ctggtattgg ttcctttgaa
     5401 tttgtctatt tgttattatt cagaccagtt gttggaacag ttgacgcagt ttcttcgctt
     5461 ttactgtatc gtttcagttc ctttatcttg ccatttctct atggatttgt gtatgtgatt
     5521 gctgaacggc gaagagtgat caaagaagaa ataatagaag taaaaaaaga gaataaagaa
     5581 aatcaagggt aggtctttta taagatctac cctttttatc aaatcaaaga aaaacgttat
     5641 ggataagata attttacgtt tgtttggctc tttttttata tacata
//
LOCUS       DAYYGO010000142         5587 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000142 DAYYGO010000000
VERSION     DAYYGO010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.145"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..375
                     /locus_tag="ACHA5T_002534"
     CDS             <1..375
                     /locus_tag="ACHA5T_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HFM8888065.1"
                     /translation="KDAISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEI
                     VPQNVSKGVVFDRLESIYNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKA
                     DYVTVSNDKDGVSQAFERILTI"
     gene            656..1876
                     /locus_tag="ACHA5T_002535"
     CDS             656..1876
                     /locus_tag="ACHA5T_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFM8888066.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            1873..4593
                     /locus_tag="ACHA5T_002536"
     CDS             1873..4593
                     /locus_tag="ACHA5T_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8888067.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            4577..5521
                     /locus_tag="ACHA5T_002537"
     CDS             4577..5521
                     /locus_tag="ACHA5T_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8888068.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
BASE COUNT         2074 a          899 c         1101 g         1513 t
ORIGIN      
        1 aaggatgcta tcagcattta caaatttttt atttttattc ctaatcgtca attgaaagct
       61 aatatattta accatttgaa aaagatgcta aatgtgtctg ttactgaatc ggcacctgta
      121 aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg tgtttgatcg tttggaatca
      181 atctacaatt taaagaaacc tttgagaatc gcgatcggag atagtttaaa tgacttatct
      241 atgtttgaaa gtgcagatat tagttttgca atgggaaata gtcatcaaga aatcatgaaa
      301 aaagctgatt atgtcactgt ttcaaatgat aaagacggag tatctcaagc atttgaaaga
      361 attcttacca tataaactaa ttaaggtagt atagaacata gaattctaaa tacggaaaaa
      421 tggagcataa attccagtac aaatagtact tcttcagtgt caaacgaagc gaatataaca
      481 tagatattta ggattgtgac aaaaatcatg tcacaatctt tttttcgaat aaacttattt
      541 tttgaagtcg gccatttatc gtaatttctt tgcataatac gaacaaattt tcggttattg
      601 atgaaaaaaa acattgaaaa tggtatgata aaagaaaaaa aggaggaaat ttttaatggt
      661 gaaatttatc catgcggcag atttacatat ggatcggtca tttgaaggac tgacaactct
      721 tgataaaact gtacaggaaa aactattgaa gacgaatctg accgttttat caaatatcat
      781 tgaacaggca ataataaata acgttgattt tgttttactt gcaggagata attttcatca
      841 aaatcgtcct tcattaaaaa tacagaaaca tttttcagaa cagatgaaac ggttagaaaa
      901 gaaccatata cctgtattta taatattcgg aaatcatgat ttctatcaaa aagagcggta
      961 ttggttctcc tttcctaaaa atgtccattt atttacatcc gaaacggtag agacaaaaaa
     1021 aataacgata aaaagtggag aaactgtctc tctcagtgga tttagttatc gtcaaccgtg
     1081 gattcaaaaa gacaaagtga tggaatttcc ttctagagaa ctcacagatt accatattgg
     1141 gttgtaccac ggggaacctg gcgtctctca aaaaggaaac tatgctcctt tccaaccctc
     1201 aaagatgcaa gaaaagggat acgattattg ggctttaggg catatccatg tgccaacggt
     1261 cttaaatgaa aagcaaacaa ttgtttatcc aggtgcgcct caaggtcata ctaaaaagga
     1321 acaagcttct acatctattc tactagttga attaagtaaa ggaagttgcc aaatacatcc
     1381 gattaaagta gcagaggtct attggaagac aaaggaaata tcattgagga aagcaagaac
     1441 gacaaaagag gtcattgttc acattcgtca gcagctttca aaagtagagg atggatttag
     1501 tttaattgag ataaaattaa aagattatga ccatctcaat acagatgttt tggaacggat
     1561 acaatcagga gagctattag actatttgtt agaggaattc tcagatagga taaatgattt
     1621 ctttatttgg cgtatttctc tcatagagaa tactttttat acgaagaccc cacttgctgc
     1681 ttctgctaaa ttgatggaac agttatttca atactatcag acacctcaag atttccaaca
     1741 aattttaaat gaagtatata gtcatcaaga agcggctaga atattgagcg aactgccgga
     1801 atatcaagaa gaaacacttg aaaaagccaa gcagctgctt aatcaagatt ttttatttga
     1861 ggaggatcag gaatgaagat tttaaggatc gagattgctg cttttggaaa atggcgtcaa
     1921 aaaagttttg acttttattc aggcaatcag ctgatctatg ggggaaacga agcaggaaaa
     1981 tcaacgattt atcaatttat tcaagcaata ttatttggtt tcccttcaaa aggccgtaaa
     2041 aaaaaggatt atacaccaaa agacggctca gcctatggcg gcaaaatatg gcttaaacat
     2101 cctgtatatg gtgaatttgc tgtcgagcgc tacaagcagc aaaatcgcgg aaaatcgaag
     2161 gtgtggctgg gggatcaagt gggaagtgat gagttactag aaaaactgat cgcacctttg
     2221 acgaaagagt tattccagca ggtatttact ttccagcaag agcagttgat ggaactagat
     2281 catttgagag aaaaagagct acatgatgcg ctgatttctt taggcatcac gggcagttct
     2341 caagtattcc aaaagcggca agaatattat cagaaagcac aacaattatt caagaaaaaa
     2401 ggccaaaaat tactaatcaa tcaaaaatta aatgaatgga aagagctgca agaaaaaatt
     2461 catcaaaaac aagaacagga agcttatttt ggcaagttga tccaacaaga acaggaatac
     2521 gagtcgcaac agcagcagtt attggcacaa ttaaaagagg cgaacaaaca attttcatta
     2581 gccagacagc aagaacttaa tttttcttta tatgaagaat ggcaatctct caaaaaaaac
     2641 agcactacag gtcttcctga tgagcaactt cttttagatc tggagtcttt ctccaaacgg
     2701 tatcagcaaa caaatgagcg tttggaagaa ttagaagcaa cactggaaaa gcatagcggg
     2761 atggatcagc agtcagccag atattatttt tatttagagc aggagcagga actaaaagat
     2821 ctccagcaac ttcaatttac agcggaacaa ttaaacgaag aatggcaaag attacaaatt
     2881 gagactagag aaacaaattc agaattgaag attttggaac aaaggtggca ttggcagaaa
     2941 aatccgttac cggaagaatt tttatatgaa gaagaatgga agcatcttct ggctgaagaa
     3001 agaaggattg ctgatctaga aagccagatt tctgtgcgtc tccaaatagc taaagaacag
     3061 caagagatga ttgaagcaga agtgaatgac ttggaagaga cctatccagc attattacaa
     3121 aatcagtcag agatagcacc agtctcttca tctaaaccgt atatgctgat ggctggcgga
     3181 gcaataatca ttgcaggtat atttgtacca atgcccttga aagtgatcct tttactaatt
     3241 ggtgcaggca tgattggcta tttcttctac aaaaggcaac caaaaattaa aactaaaaat
     3301 gatttctctg agataaaagg aatgtggcaa gaaaaactag gacaacttga tctagcctta
     3361 gctcaattag ctgaagcaga agaaaaacaa aagagaactg gagaagaaaa acagattctt
     3421 cagcaagaag tatatcgttt tgcggatcat cggcacttag gaaagatgga tacgctcaaa
     3481 ctcatgaaag aatatggaca ggaagtcata gattatcaga aattatatgg cgtatttgag
     3541 cggaaacata aaagagaaca ggaaatacaa gaaaaactac gacaaatcga ccaaaaattt
     3601 gagtttgtta aagaatggtt accgatacaa gataagacaa tttcagaaaa aatggtattg
     3661 ctttcccgtt ttatacaaga gatggaacag atgaaatttg ccagaagcta tcagcaaaat
     3721 actttattga agcaagaaat caatcaattg aaaaagaaac aaaaagtatg gatacaagaa
     3781 tacagcgaac aattgactca agcaggctta tcttatcctt cggaagtacc tgtcttttta
     3841 caaaaagcca aacaagccga acagcagatg aaaagataca atgaattatc ggaaatgctt
     3901 actccactat ttcctgaaga gctgacaaaa gagaaattat ctgtaagact gcgccaactg
     3961 aaagaaaagc aatcccaact ccagcaagaa gaaaaagaag caagtgaaaa tcggcaacga
     4021 ctccaacttc aagtagaaga gttgcaaaga gacggcacat tggacgaact ttatcaacaa
     4081 gctagccggc aaaaagcaaa attacaggaa ttactggagg aatggggagc acttgaaatc
     4141 gccgggacat tcttaggtga tctttcaaca gagatgagtg aaagacaatt accccagttg
     4201 ttaagagcag caggtgatta tttaggtatt ttaacaaaag gaaattatca gaagatatta
     4261 ctgaaaaatg atacactgac tttaacagac ggatatacag aatttcccat ctatgagtta
     4321 tctacaggta cgaaagatca gctgattatg gcgatgagat ttgcttacct ctcattagaa
     4381 gctaaccgtt cgatatgccc gatcatcatt gatgatggct ggcttcatta tgatcatcag
     4441 cgaaagtatc aattagctga acttttagct aaatttggag aaaagtatca aataatttgt
     4501 ttttcttctg ataaagaaat ggtaagctat taccatgaga tgaaccaaat cgtaaaacaa
     4561 ttagaaggag caaataatga aaaaaattcg tgatttagta atcgatgagc agtttgaaca
     4621 atttgtcctg atcaaaagtg cagatgtaag aatcgctaaa aacggaaaaa aatttattgc
     4681 ttttactttc caagatactt caggtactat cgatggtaaa tactgggatg cttcggagga
     4741 ggaaatcaat aaatttacag ccggcacagt cgtttttcta aacggtaaaa gagaggttta
     4801 tcaaggaaac cctcaagtaa agattcttca tatgcgctta acaagggtag atgagccagg
     4861 agagcctagt ttatacatgg aaagagcccc tttgaagaaa gaagaaatgg aagaagaaat
     4921 caatcaattg ttgtttgaga tcaccaatgc gcattggaat cgtattgttc gctatctact
     4981 taataaatat caaaaacaat tttttgagtt tccggcagca aaaagaaacc atcatgcatt
     5041 tgccagtgga ttagcttatc atactttgac aatgcttcac ttagggaaat caatcgccga
     5101 tgaatatcct gagctgaatc gagcattgtt gtatgctggt atcattttgc atgatttagg
     5161 aaaggtgatt gaactttccg gtcctatgtc gacagaatat acgcttgcag gaaatttgat
     5221 cggtcatctg gtattgattg acgaagaaat aactaaagcc tgtcttgcgt tgaaaatcag
     5281 cgaagctgac gaagatgttc tcgtattacg tcatatggtt ttagcacacc atggcttatt
     5341 agaatatggc tcacctgtac gtccaagaat aatggaagca gaaatattac atcaaatcga
     5401 caatatcgat gcttcgatgc agatgatgtt gacttcgatt cgtcaaacag aaccaggaaa
     5461 atacaccgat cggattttcg gtatggacaa ccgaagtttc tatgtaccaa aagatttata
     5521 gcaaaaattt atttttttca ttcaaaacaa aaaaagcgag acataagtct tcgctttttt
     5581 tgttttc
//
LOCUS       DAYYGO010000143         5447 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000143 DAYYGO010000000
VERSION     DAYYGO010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5447
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.146"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..348
                     /locus_tag="ACHA5T_002538"
     CDS             <1..348
                     /locus_tag="ACHA5T_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HFM8888069.1"
                     /translation="VYDEYSKETDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKV
                     TDEIDEHRGELIKNLVTNRAKIQTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQR
                     GIKNLVKSYLKMN"
     gene            351..1247
                     /locus_tag="ACHA5T_002539"
     CDS             351..1247
                     /locus_tag="ACHA5T_002539"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8888070.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            1297..1686
                     /locus_tag="ACHA5T_002540"
     CDS             1297..1686
                     /locus_tag="ACHA5T_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8888071.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            1673..4003
                     /locus_tag="ACHA5T_002541"
                     /pseudo
     CDS             1673..4003
                     /locus_tag="ACHA5T_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            4111..5298
                     /locus_tag="ACHA5T_002542"
     CDS             4111..5298
                     /locus_tag="ACHA5T_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFM8888072.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT         1805 a          887 c         1158 g         1597 t
ORIGIN      
        1 gtttatgatg agtattcaaa agagacagat atttataggg aactgaaacg tgatgtggag
       61 gcacttaagg aaattcccaa aaatctaact gatagtgttg acaaaattga ctttggcaat
      121 ggtataaaag ttacagacga aattgatgaa caccgcggag aattgattaa gaatttagtg
      181 accaatcggg ctaagattca aacaataatt aatgaaatgt cagatgaaat tgatagttat
      241 agagcagagc tgaataaatc agattggaaa gttaactata atgaggtttc aaagaggtat
      301 caaagaggta tcaaaaactt agtgaagagt tatctcaaga tgaattaaaa atgttacaaa
      361 accttgattc tttatatcaa tctttatcaa aaactgaaga gcgattggaa gaaattattc
      421 aacaagaaat aaagcttaaa gattaccaag ttgaggttga ctcagcaatg tcaagaatga
      481 ataaatcata tcaaaatctc tattctgaga gaagtagttc ctcgcgcgaa attttttctg
      541 atattacagg aatagaggct actattcaac caaataggga tttcaataat tatgttaaag
      601 agctcagagc ttgtattaat aaggaggaca cttttgacag tgagtttgga tctcttattg
      661 ataagctgac aaaaaattcc attactaatc aagatatata taatgacata cttgatataa
      721 gagttagtgg ggaaagtagt attttttcag ataaaaaatt aatcaattct ataagaaaat
      781 tgaatgatgc acaagttctt gaattacaaa cccttaagcc aaatgattta gtagttatcc
      841 gacttgaact aaatggaaaa aaagttagtt tatcgaatgc tagtgctggt caaaagacat
      901 ctgcaattct ttctatgatt ttggcgtatg gaaattcacc actcatattg gatcagcctg
      961 aagatgattt ggatagtcaa ttaattaata acttaatcgt aagaagtatc attacaaaaa
     1021 aggaaaacag acaaatttta attgtaactc ataatgcaaa tatcccagtt aatggtgatt
     1081 cagaatggtt gattagcatg ggagattcaa aagaaatatc ggctgatcag tgcgggtcgg
     1141 tagatgaacc caatatcaag ccaagaatat gtactgtgat ggaaggtggc gaggatgctt
     1201 ttacaaatag agcgaaacgc tatggtttta aacaaattgt agaataacac tagagggtga
     1261 gacatattat tatgtccgcc ctttttagga ggaaaaatga aaaaaataaa aagctatacc
     1321 agtatctggg cagttgaaaa agtcatttac gcaatcaatg attttcagtt gccttttcct
     1381 gtgactttta accaaatggc ttggttcgtt ttatctttac tgtttgtcat cgtatttgcc
     1441 catgtgcctc cactatctat gatagaggag gtctttttaa aatacttagg cataccggtg
     1501 gctgtcactt ggtttatgtc gcaaaagacg tttgatggca agaaaccact gggattttta
     1561 cgatccttta tcagctatca cttgcgattt aaggtgactt ttgcaggaaa gaaagtcaaa
     1621 gaacagaaga aacgatggga tgaacccagt actcttgtaa ggagtgtgaa ctatgtatcc
     1681 gattaagtat attgaaaata atctcgtctt taaccaagaa ggcgagtgtt ttgcctacta
     1741 tgaattagtg ccttacaatt actccttttt gtcacccgaa cagaaatttc aagtgcatga
     1801 taactttcgc caactaattg cgcaaaatcg tgaaggcaag atccatgcgt tacaaattgc
     1861 cacagaaagt agtattcgcg ccacacaaga acgatcaaaa aaagaaatta ccggtagact
     1921 caaagaagta gcgaaacaac gaattgatat acagacagat gcattagtat ccatgattgg
     1981 cgatagtcag attgattatc gcttttttat tggctttaaa ctaattgcga cagatgaaga
     2041 agtcaatcta aagagtctca agaaatcttt cttttctggg cttcaagaat ttgtctatgg
     2101 agtgaatcat catttgatgg gcgattttgt ttccttatcc aatgaagaaa ttcgctgcta
     2161 ttctaagctt gaaaaattaa tggaaagcaa attggctcga agatttaaag tcaggcgagt
     2221 cacaccgagt gacctgacgt atttgattga acatatttat ggcgaaaaag ggataccgtt
     2281 tgaagaatac gaatttcagc taccaaagaa aaagctaaaa tcagaaacac tggtgaaacg
     2341 ttatgactta ttacgtccaa atcgttgttt aattgaagaa aaaccaaggt gcttacggat
     2401 ggaacatgag aatcatgagt cgtacgtagc gtatttaaca attaatacaa ttgtggggga
     2461 gatggaattt ccttcctcag aacttttcta ttaccagcaa caacaattta cattcccaat
     2521 cgacacaagt atgaatgtag agattgtgac gaataaaaag gctttagcta ccgttcgtaa
     2581 taagaaaaaa gaactgaaag atttagacaa tcatgcttac caatctgaca atgaaacgaa
     2641 ttccaatgtc ttagatgctt tggattccgt ggatgaattg gaaaccacct tggatcagtc
     2701 aaaagaatct atgtacaaac tgagttatgt ggttcgagtg agtgccgaat cagtggatga
     2761 actaaagcga cgctgtgatg aagtgctcga tttttatgat gataccaatg taaaactagt
     2821 ccggcccttt ggcgatatga tggggctaca tgaagaattc ttaccctcaa gtaaacgata
     2881 tatgaacgac tatattcagt atgttacgtc tgattttttg gcggggcttg gttttggggc
     2941 tactcagatg ctaggagaat tagaagggat ttactttggc tacaatgtgg atacaggtcg
     3001 taatgtttac ctgaaaccgg cattggcttc acaaggagtc aagggttcag taaccaatgc
     3061 gctggccgcc gctttcctgg gttctcttgg tggaggtaaa tcctttagta acaatctctt
     3121 agtctactat gctgttttgt tcggtggtca agctgtgatt gtcgatccaa aaggggagcg
     3181 aggagggtgg aaagaaacct tgccagaaat tgcggatgaa atcagtatcc tcaacttgac
     3241 gagtaagtca gaaaatcaag gtctgcttga tccttatgtg attatgaaga aaaagaaaga
     3301 ctcggaaagt ttagcaattg atattctgac ttttttaaca ggaatttcaa gtcgagacgg
     3361 ggagaagttt cctgtgttac gtaaagccat tcgaacggtg actcaaagtg atcaacgggg
     3421 gttattacaa gttattaacg aattgcgaaa agacgggtca ccaatcgcgg aaaatatcgc
     3481 cgatcatatc gagtccttta ccgattatga ttttgctcat ttgcttttct cagacggctc
     3541 gattacccaa tcgattagtt tagataaaca gctaaatatc attcaagtgg cggatttggt
     3601 tttaccggat gcagaaacga cttttgaaga atataccacc atggaattgc ttagtgtttc
     3661 gatgctaatc gtcatttcca cttttgcctt ggactttatc catagtaacc gagaaatctt
     3721 caaaattgtc gatttggatg aggcttggag ttttcttcaa gtggctcaag gaaaaacgtt
     3781 atcgaataag cttgttcgtg ctggtcgggc catgaatgct ggggtctatt tcgtcactca
     3841 aaatagcgac gatttattag atgaacgttt aaaaaataat atcgggttga agtttgcttt
     3901 tcgcagtacc gatattcatg aaatcaagaa aaccttggaa ttctttggtt tggatcagga
     3961 ggatcaaagc aatcaaaaac gcctgcgaga tttggaaaac gggattacgt ccatataatt
     4021 gtgtaaaagt gaagcatcaa aaaagccatt gatctcagtt aggagtacaa tgttttttgc
     4081 agaaaacata ctcgaatagg actgatatca atgactcaag tacattttac ttttgaaagc
     4141 gaagaaattc aagcaattat aaacgaaagc ggtgcgaatg ataccgcaaa aacacttatg
     4201 actatcatgt ttaatcagct gatggaagaa caacgaaatc aatatattca ggcaaatgcc
     4261 tatgaacgtt cagaagaacg gcaatcacaa agaaatggtt actacgatcg ttcgtttaca
     4321 actcgaattg gtacgttaga acttcatgtt ccacgaacca gagacggtaa gtttagtcct
     4381 actatctttg aaagatatca gcgaagcgaa aaagctttga tcgctgccat gattgaaatg
     4441 gtcatttctg gtgtctccac tcgcaaagta actaaaacag ttgaactgct aacggatggt
     4501 gcgactgttt ctaaatcatt cgtttcaaat ctgatgaaac agttggatcc atttgttttt
     4561 gagtggagaa accggagcct agaaggctcg gaatatccat ttttcatgtg tgatgccctg
     4621 tatatgaaag taagggaaaa tcatcgtatt gtctcaaaag gtgtatatat tggtatcggc
     4681 attgattctg atggacgacg tacgattctt ggttttgatg ttcaagacgg cgaatcggaa
     4741 gataattggg ataccgtttt tcaatcgttc gttcaacgag gtttgttcgg tgtaaaactg
     4801 gtcatttctg atgcacacaa agggttagtt aaggctgttc gcaagaactt tttaggtgcg
     4861 agctggcaaa gatgccaagc ccactttttg agaaatattt ttgataagct gccaaagaaa
     4921 gtttcttctg atgtaaaaga cgagttgaag agtatcttta aagcctctga acttgaattg
     4981 acacgtgaaa gaaaagagca cttcttggaa aaatatggtt gtgattcaaa attaagtgct
     5041 gcttgcgaca ttttagaaaa cggctttgaa gatgctattc aaattttgtc ttttcctgaa
     5101 aatatccgcc gaaggattcg tacaaccaat gtgttggaac ggttgaacga agagattcgc
     5161 cggagagaac gagtgattcg gatttttcca aatatcaatt ccatcactcg gataattgga
     5221 acacttttga tggaaaaaga cactgagtgg ctggcttctc cacgaaaata tttagaattt
     5281 aattcgaata acatttagca ccatgaacct gtgcgagtct cttctttctg aggagagaca
     5341 tcaagagtcg ctgcgctact gctcctgacc tctctcctca gaaagaaagg catggcagtt
     5401 aggttcacaa cgcaaaacat tgtgcttttg atgaggttga actttta
//
LOCUS       DAYYGO010000144         5442 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000144 DAYYGO010000000
VERSION     DAYYGO010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5442
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.147"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..569)
                     /locus_tag="ACHA5T_002543"
     CDS             complement(<1..569)
                     /locus_tag="ACHA5T_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HFM8888073.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
     gene            complement(903..990)
                     /locus_tag="ACHA5T_002544"
     tRNA            complement(903..990)
                     /locus_tag="ACHA5T_002544"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(956..958),aa:Ser,seq:cga)
     gene            complement(1135..1701)
                     /locus_tag="ACHA5T_002545"
     CDS             complement(1135..1701)
                     /locus_tag="ACHA5T_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8888074.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            1956..3287
                     /locus_tag="ACHA5T_002546"
     CDS             1956..3287
                     /locus_tag="ACHA5T_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HFM8888075.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGVFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSVLSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGILLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTT
                     DFIANSGIGATLINMGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            3253..3603
                     /locus_tag="ACHA5T_002547"
     CDS             3253..3603
                     /locus_tag="ACHA5T_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888076.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(3695..4114)
                     /locus_tag="ACHA5T_002548"
     CDS             complement(3695..4114)
                     /locus_tag="ACHA5T_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFM8888077.1"
                     /translation="MMTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(4115..4459)
                     /locus_tag="ACHA5T_002549"
     CDS             complement(4115..4459)
                     /locus_tag="ACHA5T_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFM8888078.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVEWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            4809..5405
                     /locus_tag="ACHA5T_002550"
     CDS             4809..5405
                     /locus_tag="ACHA5T_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM8888079.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1513 a         1008 c          930 g         1991 t
ORIGIN      
        1 ccatttgata gcgagcgata attgttcaga ttaaataatt gataatcaaa tggcggatta
       61 tttgtcaaca caccgacagg attatcatat aaatgaagtc catctttcat actttcaatt
      121 actatcgatt tttctttatc agctaataac cagtgtaaag gtgacaaggg tagtttttca
      181 ctaaaatcga tattgactag attgatagta gccaataatt ctttagcttc gtctatggtt
      241 gagcattgcc ctagtatcca tggaataaac tcaaatggtg caacgttatc ttttccttct
      301 tgtaattctt tataatcagc attgccagga aaattcagtc ctgccatact caaccctttt
      361 tcatttgtag cctcataata gagtggatag ttggataccc cagctgctat tcctatcatt
      421 gcataatgtt tatctaaatt ttttaccttt ctaaaatcga aacgataatt tcttggagta
      481 atagtgacca cttcattata agacatctca taatcaaaat tccttccaaa ataatggtct
      541 ttcgttgcat aagtgattgc ggtacacatc gtttcccctc ctaaaacaaa tcgtttttac
      601 acttttatct tacatctagt atttttttta tcaaaaaaag gccaaagaag attgatttac
      661 tagcatttat aaaaaatagt actcaggggg aatcaaaaaa tattgtaggt taaaagtaca
      721 acaataaggg gctgtgacaa aagtcttgtc acagcccctt attccgaaca aacggtgttc
      781 aaaagcttgc atctgcggta ataagcccaa tcaaacgaaa aaataggaaa agctattttt
      841 gtttggccgt tcttattact tgatgcagga cagcttcttc acaacctcta atcgttgata
      901 taaggaaggt acaggatttg aacctgcgcg ccggtattag ccggttcgcc ggatttcgag
      961 tccggtgcat taccactctg ccaaccttcc gtaagtacta tttttaaact tttttcacga
     1021 aatgtcaagc ctatttctaa tagataaata acagaaatcg ctttttttag caaaaaagtc
     1081 cattgacagt ttgcagccaa cggacttacc gatcaaattc atactgatag aacactatct
     1141 gattatttca ggattatcta atgctaaaat cgagtcaatt gtccgtttga tcagtgcaat
     1201 gatttgttct tctgttggat gcatgatgtt tgatgacatc aatgacgcta agccagtcac
     1261 tgctacccat gtaccagtat gcagagcatc gacatattca tccgataatt gattgtatct
     1321 ttcatcttct ttaactattt cacggaaata attgtatgaa aaatcatgca ttttctttcc
     1381 gtccccatgt tcatcaatgt acaatgccat gtaaagacta tgatgctttt ttgcaaagta
     1441 gatatagttc aaaccgagat caatgatttt atcaccagta cgttcaatag ggaaaacatc
     1501 ttcttttagt tgttcaaaga tagaatccag caacgtattt tttaaatctt ccatgttttt
     1561 gaattccaga taaatcggct gtgtcgaaat ccccatcttt ttagcaatat ttctcgcagt
     1621 aaatccttta aacccttcct ttgcaacaac atcataagca gcttttagaa tttgatcctt
     1681 ggtatagact tttcttctca ttaaaactcc cccacttctt tactgcagta tatgttgttt
     1741 tattgtaccc tatttttagc attttttgca aacattttac tcaaaacgtt atattttaaa
     1801 tttttttcag tcttctttaa cttttttttg caaaattaaa aaaatttggt taaattcaat
     1861 gggttccctt acatttttag ttttgaccta aatgatagtt gtccaaatct ttcatttcaa
     1921 gttatagtta aagaaaatga aaaagaaggt gtgtaatgga aaacgaaact gacgttattt
     1981 atattcatcc tcaaaaacga atcgtttcac agaaaagaaa gtatttttat cttggtttta
     2041 caggtgtttt ttttcttttt attggcttgc tttctaatac tcctactgat aattggagtg
     2101 gattgctaac tattcttacc tcgccaagca atcttttgac agattatttt gctttaggtg
     2161 ttttcggaag cgcattcata aatgtaggga ttttgacctt actttctgtg cttttagctt
     2221 atcggcacaa ggtgatttta aacggtccct tatttgcttc gattctaaca gttaccggtt
     2281 tctctttttt tggcaaaaac ctttataatt ctatctcaat cattttaggt gtgtatctat
     2341 acgctgtatt tgtcaataaa cctttttctc agtacatcat gatcggactg tttggtagcg
     2401 tattatcacc ggtcgttagt tatatcactt ttggtatgcg ttttccttta ctgataggta
     2461 ttttattggg gaatttagct ggcatagcaa ttggtatact tcttccgccc ttagctgctc
     2521 aaactttagt tttccatcgt ggattcacac tttataatat tggattcacc tctgggttga
     2581 ttgcgatgac ttttactgct gttttgaggt tattcagcta ttcaatcgtc gaaaatacat
     2641 tggttttcaa tgaatatcac tttcctctca tttggattat ttttggcttt ttttcgctga
     2701 cagtcggaat tggtttctat tataattcct ttcggctttc ggggattcga gaaattttcg
     2761 attcatctgg aaaattgaca actgatttca ttgctaattc tggtattggt gctactttga
     2821 tcaacatggg cttagttggt ctgatgctgt cttcttatgt tttgctggtt ggcggtcagt
     2881 taaatggacc tgtcatcggt gctattttat ctgcggtggg tttcagtgct tttggttgtc
     2941 atttaaaaaa cagttttccg atactagtcg gaatatttat tgcttctttg tttggaactt
     3001 ttcatgagat caccagtact ggcatgcttg ttgcagctgt gtttgggaca ggtcttgcgc
     3061 ccataagcgg tttttacggc agtttttacg gtgtaatagc tggtgtgcta catattgctc
     3121 ttgtccataa cgtcagcact ttgcacggag gactaaatct atataacagt ggtttttcaa
     3181 ctggtttcgt tgcaggtatc cttgtaccga tactagataa ctttaccgct gtcagaaagg
     3241 aaaaaaagac acttggaaaa agaattatca aaaagaatca tcgatgagct tcttacttat
     3301 ttatattccc atgaaataac tgaaatacgt attggagtag aatttgctga ggaagggctt
     3361 ttcatagaga tacagggtac ggcagaaact cagccagaag atttagatgt acttgctgat
     3421 ttgctgaaca atcctcggga ccctagtatg gaaatctact atcaagaact attaggtaat
     3481 ccccgacatg atcgggaaga ttatcatctg ttaggtagtt tgattgatga cgtggaaatc
     3541 atgtatgccc agccgattat caatattaag atctttcgac gtgcttctca ttcctattat
     3601 taaccctatt ttctatttat aaaagactga atcaaaaaag cagctcctga aagttcgatt
     3661 aaaatctact ttcaggagct gttcaagctt gacattattg attttccaat ccttcataca
     3721 cttcatctgt tacaggttct aaaaactctg aattaccagc agtgatcgcc aaatgttcaa
     3781 accaactgtc ttttgtcgca ccatgccaat gtttaatccc tttgtgcgtt acgacgacat
     3841 ctccagccat taatcgtctt gctggctttc cttcttcctg ataccagcct tctcctccgg
     3901 tcacaagtaa gatttgatag ccgtctgtat ggacatgcca gtgattgcga caacctggtt
     3961 caaaagttac attacctaca ccaacagaaa cttcgggatc attcactaac atattcagat
     4021 agctttgccc ggtaaatact tgagcgtaag cttcgttttt ttcacctgct ggaaaaatca
     4081 cgccattgtg tatttcttcg tgttttgtca tcatttacgc ctctttctta tcttcgctat
     4141 tttcttctaa atagatttct tttgctaaac taaaagctga ccaagctttt ggccattcta
     4201 cataaaaagc taaatgggta atgatttctg ctatttcttc actagtaatg ccattttgct
     4261 tgccaatctt cagatgagct ggcaattgtt caaaatttcc tcctgaaatt aatgcagata
     4321 tggtgatgat actacgctga tgagcagata attcttctct ggaccatact tgtccaaata
     4381 aaacatcatc atttaattct gcaaattttg gtgcaaaatc tcctaattga tctcgtcctg
     4441 cggtttgttt ttctgccatt tctcttattc tcccttcacg actgttaggt gattcaacta
     4501 tggttctaag gcaactttat tttctattgt ttcttcgcat ttcattttaa atgtagtctt
     4561 tcagcctgtc caatgcttag tttattcagt cgatatgctt tttaagcata gttgctaacg
     4621 ctttttttat tttaaaaaaa acaagttatt aaataagtct ttagttggat gtttttttga
     4681 gatcaatctt ttatacttat gatgaactta caaaattgtt tgttttctgt catatcaaaa
     4741 aatggcgcag ttagttgctt ttctagtaaa gtatagttaa caaagaacct ctaaggagtt
     4801 gatgaataat gaatattgct gcctctcgaa aactgataaa tttcatatcc attatcggat
     4861 taggcctatc aatcgcgctg acgatttatt ttattaattt aggtgttttc aaagatttga
     4921 atgcgttaag aggattagta ggtgattcca tcattttagg accgattatt tttgtcttca
     4981 tccaaatcct gcaagtagtt attccaatca ttcctggagg gatcagtact gcagctggtg
     5041 tcctgatatt tggcccttat gctggattta tctataacta tattggaatc tgcattggtt
     5101 cgattatcat ttttctgcta gggcgtcgtt atggaaagcc attcatactt tccatgatta
     5161 gcgataagac atataacaaa tatattggct ggctggataa tcaaaaccgt tttgagaagc
     5221 tgtttgctct agctattttt cttccagttg ctcctgacga tgcgttatgt ttgatggctg
     5281 gattgacaaa catgtcagtg aaacgatata cactaatcat tttgattgct aaaccactgt
     5341 cgattttctt atatagtatg gccttgattt atggaggtca atatctaagt ggtcttcttg
     5401 gataatattt attttgtaaa attagtcatt gattgacaat gt
//
LOCUS       DAYYGO010000145         5328 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000145 DAYYGO010000000
VERSION     DAYYGO010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.148"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..165)
                     /locus_tag="ACHA5T_002551"
     CDS             complement(<1..165)
                     /locus_tag="ACHA5T_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8888080.1"
                     /translation="MSRRQRRTYSKEFKQQIVDLYLAGKPRAEIIREYELTPSSFDKW
                     MKQAQSTGSFK"
     gene            complement(367..762)
                     /gene="panD"
                     /locus_tag="ACHA5T_002552"
     CDS             complement(367..762)
                     /gene="panD"
                     /locus_tag="ACHA5T_002552"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFM8888081.1"
                     /translation="MKVTMLKGKIHRATVEQAELDYVGSITVDMNLLEAAGIYEYEKV
                     QIVDVNNGNRFETYTIAGERGTGMICLNGAAARCVSTGDKIILMAYCELNENEVKDHR
                     PKVVFVDDNNKVERVTSYEKHGRLSDIIL"
     gene            complement(772..1620)
                     /gene="panC"
                     /locus_tag="ACHA5T_002553"
     CDS             complement(772..1620)
                     /gene="panC"
                     /locus_tag="ACHA5T_002553"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFM8888082.1"
                     /translation="MVIFKTVNDVRSQVKEWKKQGLKVGLVPTMGYLHEGHESLIRKA
                     SKENDKVVVSIFVNPTQFGKNEDLGSYPRDLERDIEVCTRGRATAIFNPEVEEMYCDN
                     ASTFVNITGLTEGLCGASRPIHFRGVCTVVSKLFNIIPADRAYFGEKDAQQLAVIKRM
                     VRDLNIDIDVVGCPIIREEDGLAKSSRNTYLSLEERSSATILNKSLTLAKEALNNGER
                     DSLKIIEIISKNINTYNLAKIDYVEVVDSLSLQRVNYIEKSVLVAIAVFIGKTRLIDN
                     FTFELQ"
     gene            complement(1635..2462)
                     /gene="panB"
                     /locus_tag="ACHA5T_002554"
     CDS             complement(1635..2462)
                     /gene="panB"
                     /locus_tag="ACHA5T_002554"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFM8888083.1"
                     /translation="MKNTAVTFKESKLRNEKLTMLTAYDYSTAKIIDEAGINGILVGD
                     SLGMVCLGHEDTLSVTMEDMIHHTRAVTRGAKNTLVVADMPFMSYQTSVYDSVVNAGR
                     LIKEGRAQVVKLEGGIEVCDKIEAIVKASIPVMAHIGLTPQSVNAFGGFKVQGKDKEA
                     AKELIRAAKAVEKAGAFAVVLECVPTKLAELISKEISIPTIGIGAGAGCDGQILVYQD
                     MLGMYSDFTPKFVKKYANLSEEMNKAFTKYIEEVKDGVFPGPEHGFKISDDVLEKLY"
     gene            complement(2474..3334)
                     /locus_tag="ACHA5T_002555"
     CDS             complement(2474..3334)
                     /locus_tag="ACHA5T_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann-like and DUF2520 domain-containing
                     protein"
                     /protein_id="HFM8888084.1"
                     /translation="MEVIIMNVGFIGAGKVGCSFGKYFQEHKIQVTGFYSKSEDSSLA
                     ASNFTSSKQYLNLRELVDENDTIFITTPDGQIQEVWQEIKNYQIKNKLICHCSGAISS
                     DIFSNIQDYGAYGYSVHPMFSISDKYNSYKKLKKSFITIEGDEKYAKFLCDFFKKLGN
                     STVIVSKENKSLYHAASVVSSNLVLGLINTSVTYLIQCGFTEKMAIEALYPLIEFNIR
                     NIKEKGIIESLTGPVERCDISTIKGHCEVLSDEDRALYMLLSKNVLEIAMLKNINRDY
                     SELEKYLGES"
     gene            3572..4741
                     /locus_tag="ACHA5T_002556"
     CDS             3572..4741
                     /locus_tag="ACHA5T_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM8888085.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRGRKEICEDFKLV
                     YQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIYSTNLIEG
                     FNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTLESMI"
     gene            complement(5066..>5328)
                     /locus_tag="ACHA5T_002557"
     CDS             complement(5066..>5328)
                     /locus_tag="ACHA5T_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFM8888086.1"
                     /translation="NAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIFDVKFD
                     LNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
BASE COUNT         1729 a          992 c          646 g         1961 t
ORIGIN      
        1 tttgaatgag cccgttgatt gtgcttgctt catccattta tcgaaagaag aaggcgtaag
       61 ctcatactct cgaataattt ctgcgcgagg cttaccagcg agatagagat cgacgatttg
      121 ttgtttgaat tcttttgaat aggttcttcg ttgacgtctt gacatgaaaa atcctccagt
      181 gtgtttttta ttattctaca caccttattt tttctgtcta gtctagtgta gccgattcac
      241 ataaaatgga ctttgtctac agtctgtatc ttctcttttt gtgtctactt tattgacatc
      301 tatccaatta gtaatcaata aatatttcaa taatagtcta gctatatata aataacatta
      361 caaaacctaa agaattatat ctgataacct tccatgtttt tcatatgatg ttactctttc
      421 aactttatta ttatcatcga caaaaacaac cttaggtcta tgatccttaa cctcattttc
      481 atttaattca caatatgcca ttaaaattat tttatctcca gttgatacac atctagctgc
      541 agctccattc aagcaaatca tccctgttcc tctctctcca gctattgtat aagtttcaaa
      601 tctatttcca ttattaacat caactatttg tactttttca tattcatata ttcctgctgc
      661 ttctaataaa ttcatatcaa cagtaatact tcctacataa tctaactcag cttgttcaac
      721 tgtagctcta tgtatttttc cttttaacat tgtaactttc attatagccc cctattgtaa
      781 ttcaaatgta aaattatcta ttaatcttgt ttttcctata aatactgcta ttgcaactaa
      841 aactgatttt tcaatataat ttactctttg taatgataaa gaatcaacaa cttctacata
      901 atcaatcttt gccaaattat aagtatttat atttttactt attatctcaa ttatttttaa
      961 gctgtctctc tctccattat ttaaagcttc cttagctaaa gttaaagatt tatttaaaat
     1021 agtagctgaa gatctttcct ctaaacttaa gtatgtgttt ctagaacttt tagctagtcc
     1081 atcctcttcc cttataattg gacaccctac aacgtctata tcaatattaa ggtccctaac
     1141 cattctttta attacagcta actgttgtgc atccttttct ccaaaataag ctctatctgc
     1201 tggtattatg ttaaataatt tacttacaac agtgcaaacc cctctaaaat gtattggcct
     1261 actagctcca cataaacctt ctgttaatcc agttatatta acaaaagtac ttgcattgtc
     1321 acaatacatc tcttcaactt caggattaaa tattgctgtt gctctacctc ttgtgcatac
     1381 ttctatatct ctttctaagt ctcttggata actacctaaa tcttcatttt tcccaaactg
     1441 agttggattt acaaagatac ttacaacaac tttatcattt tcttttgatg cttttctaat
     1501 taaactttca tgaccttcat gtaaatatcc catagtagga actaatccta cctttaagcc
     1561 ttgttttttc cactctttta cttgacttct aacatcattc actgttttaa atataaccat
     1621 atttcttgac ccttttaata taatttttct aaaacatcat cacttatttt aaacccatgt
     1681 tctggacctg gaaaaactcc atcttttact tcctctatat attttgtaaa tgctttattc
     1741 atctcttctg ataaattagc atatttcttc acaaatttag gggtaaaatc actatacatc
     1801 cccaacatat cttgatatac aagaatttgt ccatcacacc ctgcacctgc tccaatacca
     1861 attgtaggta ttgatatctc tttagaaatt aactctgcaa gcttagttgg aacacattct
     1921 aacactacag cgaatgctcc tgctttttct acagctttag cagctcttat taattcttta
     1981 gcagcttcct tgtcctttcc ttgtacctta aatcctccaa atgcattaac agattgaggt
     2041 gttaaaccta tatgcgccat aacaggtata gaagccttta ctatagcctc tattttatcg
     2101 catacttcta tccctccttc taatttaaca acctgagctc gtccttcttt tattaatctt
     2161 cctgcattaa ctactgaatc atatactgaa gtttgataag acataaatgg catatctgca
     2221 acaactaaag tgttcttagc tcctcttgta actgctctag tatgatgaat catgtcttcc
     2281 attgttactg aaagtgtatc ttcatgtcct agacatacca taccaagaga atctcctact
     2341 aatattccat ttatcccagc ttcatcaatt atttttgctg ttgaatagtc ataagctgta
     2401 agcattgtta atttttcatt tcttaattta gattctttaa atgtaactgc tgtatttttc
     2461 atctatttct ctcctaactc tctcctaaat acttttctaa ttcactataa tctctattaa
     2521 tattttttaa catagctatc tctaatacat tttttgataa tagcatatat aaagctctgt
     2581 cttcatctga taaaacttca cagtgacctt taattgttga aatatcacaa cgttcaactg
     2641 gccctgtcaa actttctata attccttttt cttttatatt tctaatatta aattcaatta
     2701 atggatataa agcttcaatt gccatttttt cagtaaatcc acattgaatt agatatgtaa
     2761 cgcttgtatt aataagtcct agcacaaggt tactagatac tactgatgcg gcatgataaa
     2821 gagatttatt ctccttagat acaattacag tactattacc taactttttg aagaaatcac
     2881 ataaaaactt tgcatatttt tcatcgcctt caattgttat aaaagatttt tttaattttt
     2941 tgtatgaatt atatttatct gatattgaaa acataggatg gacagaatag ccataagcac
     3001 catagtcctg tatatttgaa aagatatctg aagatattgc accactgcaa tggcagatta
     3061 atttgttttt gatttgataa tttttaattt cttgccatac ctcctgaatt tgaccatcag
     3121 gagtcgtaat gaaaatagta tcattttcat ctactaattc tcttaagttt aaatattgtt
     3181 tactagatgt aaaattcgaa gctgctaatg acgagtcttc gcttttacta taaaatcctg
     3241 ttacttgtat tttatgttcc tggaaatact tcccgaatga acaacctacc ttgccggctc
     3301 caataaaacc aacgttcata attatcacct ccataaagaa gtgagactac aagtcccatt
     3361 cttacgatat gagcttaaaa ataaaaatta aatcaaatat acagtatgtt tttttcaaat
     3421 atcatccata gttaatttat cataatatcc catattttaa aataaaagat agcgtaaaat
     3481 attttgtgta aatagaaatt tagggtagaa aaaaaagtcc attctgtaaa attaaagtta
     3541 ccacacagaa attttatagg aggactttct catgactaaa tttactatag aaattatgca
     3601 aacactactc aataaagggg atttagatga attatttcag gatcatttag agcgagcgat
     3661 taattcactt ctacaagcag agctaactgc ttttttagat tacgaaaaat atgatcgaaa
     3721 tggattcaat tcaggaaatt cccgtaacgg gaattattca cgtacattta aaacagaata
     3781 tggcgagttg aatttaacta ttcctagaga tcggaatggc gaattttctc aacaaacact
     3841 accagcatat aagcgaacca atgattcatt agaaacgacc attatccaat tatttcaaaa
     3901 aggcattact atggctgaaa tctccaaatt aattgaaaaa atgtatggtc accactatac
     3961 gccacaaaca atctccaaca tgagtaaatt ggtggctgaa gatgttttag cttttaaaga
     4021 aagaacgtta gaagccaatt attccgttat atttatggat gcgacgcata tccctgtgaa
     4081 gcgacagact gtttcgaagg aagcagtgta tattacgatt ggtattcgtt tggatggaac
     4141 caaagaagtt cttgggttta ctattgcccc aactgagtct gcttatattt ggaaagaagt
     4201 tcttcaagat cttagaaaac gtgggttaga agaagtttta ttagtagtga cagacggatt
     4261 aagctgtatt gaagaaagta tccatagtgt gtatccgaat gcccaatttc aacaatgttg
     4321 tgtgcatgta tctagaaata tcgctcataa agttcgtgtt cgaggtcgaa aagaaatttg
     4381 tgaggatttc aaattggttt accaagcgaa ttcaaaagaa gaggcattgg atcacatcga
     4441 ctttatgact aggaaatgga aaaagcagta tccaagagtc gtcaatttac tcttgaatcc
     4501 tgccctatta accttttata atttccctca cgccatcaga cgaacaattt attcgacgaa
     4561 cctgattgaa ggctttaata agcagctaaa acgatatact cgaagaaaag aacaattccc
     4621 taatgaagaa tctctagaga gattcttcat ttctcaattt aatcaatata accaaaaatt
     4681 tttaggtaga attcacaagg gatttaaaga aattcaggat acattagagt cgatgattta
     4741 actgtaatta acagaatgga ttttccattt acacataatt cttgacgcta ccataatcta
     4801 aataaccatc atattcatct actacttata taattgagtt ttattaaact ttttaagttt
     4861 ctattctatc aaatatttaa acaatctctg catccgaaaa gactacctcg tatagtttta
     4921 ctaacaatat ttttattagc gtgttcaaat cctgatttct tgtatcaatt attttctgtt
     4981 cactttcaat aagaaaaaag tctttacttc catggataat aataagtctc tatttttcaa
     5041 taactattgc aaaattatat cttaattaag aaaatatttt tattttaaag ataaaaaatt
     5101 cttcatcctg caatacttta tattctatat tgtaattatt cagaatatta cttacaatat
     5161 ataaacctaa tccattacta ttttccttat ttaaatcaaa tttaacatca aatattttat
     5221 ccatattcga aattttattg ttaccgtatg aattctctat atataaccaa tcattaacta
     5281 tcccaatatt aattacccca tttacatcag tatattttac cgcattac
//
LOCUS       DAYYGO010000146         5258 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000146 DAYYGO010000000
VERSION     DAYYGO010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.149"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..207)
                     /locus_tag="ACHA5T_002558"
     CDS             complement(<1..207)
                     /locus_tag="ACHA5T_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888087.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSEN"
     gene            complement(505..1479)
                     /gene="sgrA"
                     /locus_tag="ACHA5T_002559"
     CDS             complement(505..1479)
                     /gene="sgrA"
                     /locus_tag="ACHA5T_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFM8888088.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(1675..2514)
                     /locus_tag="ACHA5T_002560"
     CDS             complement(1675..2514)
                     /locus_tag="ACHA5T_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFM8888089.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            2699..3586
                     /locus_tag="ACHA5T_002561"
     CDS             2699..3586
                     /locus_tag="ACHA5T_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFM8888090.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLIDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(4180..4875)
                     /locus_tag="ACHA5T_002562"
     CDS             complement(4180..4875)
                     /locus_tag="ACHA5T_002562"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFM8888091.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(4859..5257)
                     /locus_tag="ACHA5T_002563"
     CDS             complement(4859..5257)
                     /locus_tag="ACHA5T_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFM8888092.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         1671 a         1061 c          774 g         1752 t
ORIGIN      
        1 gttttctgaa agaaaagata atttatattc tggatacctt tcgcaaaacg attctgcttg
       61 ttgaatcacc gaattcatct tagccagggt gtagtttaga tctaaagcga tatccttcac
      121 cacatataat ttctcgtctc tttcataaca atagttcaaa aattgatata cttgcctttc
      181 attgatatca ataaattctt caaacaaagt acatactcct ttttatctat tatttagact
      241 tattccgaca gctacatgaa taacgtacaa atagattcgt tactgatttt tcagagatac
      301 taccatgcgc agtgtgcatt gtcaattatt cttgctctta ttttccattt gtgttcctgt
      361 tatttcctgt ttctcaaata tgttagtaaa caaaaataga tctggcaaag ctacaacttc
      421 gccaggcaat acagcactct cgaatgaaat attcactcaa aaatctatcg ttttattcaa
      481 ttttagatct gttgctttta ttctttattt cactcgatgg aagagagaac gaaaatagct
      541 tgctcctaca atcaaaatga gtaacacacc catccatgta attagcttgt tcaaggttct
      601 actaccagtt tctggtaaat cgctttttct attatctgtt tctttcgctt tttcttgatg
      661 atcaattcca cgagaaacaa aatcaggctg ttttagcaca ctttttaggt tgctccctgc
      721 atttctcttt tgttccttag ttggtatgat aggtaccaaa tcaagttcat tatttacttt
      781 tcctgtgctg ctttcgctgc tcgtgcttcc tgtgctactc tcactactca tgcttcctgt
      841 gctactttca ctactcgtac tctccgtact gctttcgctg ctcgtgcttc ctgtgctact
      901 ttcactactc gtactctccg tactgctttc gctgctcgtg ttttctgtgc tactttcact
      961 gctcgtactc tctgtactgc tttcaccgct cgtgttttct gtactgcttt cactacttgt
     1021 gctatccgta ctgcttggcg gtccaacaac tgtccaactg cttggtttca aattctccgt
     1081 gatcgtcatc tgtccatcat aaaaaacaat caaatcaact ttatctttac tcttgatctc
     1141 atcaatcgat agttctgcag aaatcattat tgtatactca ataccagcgg tccattgtac
     1201 gtcttttaaa taaacttccc caataacttt ttctgaactt ccattaccat cccaaccata
     1261 tgttagaaca gcatgtacat cactagcatt cacgcttttg tttggtttga ctggaatagt
     1321 aatgacttgt tgtgtcatgt cataaacaat ccgatcagtt ttcacagtaa atgaaagatt
     1381 ctcatttgcc cgttcatttg cagaagtact gattccaatc attgctgcaa ttatactcag
     1441 cccaagaata acaaataata cggtcgctgt tttcttcatt tctatcactc tccatataat
     1501 taccttctat ttgataaagg aattatataa aatgaaaatg atgagaacac gacagtcagt
     1561 tgtgaaaatc taacaaaaga tgtcgtgtat tcgcttgaat ttgtttactt tttcgtttaa
     1621 tcaatgacag acttaaaaaa gcaagaccga tatcagcctt gctttcttca tcttttattg
     1681 ataaacagca gtgtatttct tcatccgctt tgttttatat gcatagagta acaaaccaac
     1741 aatcaagact aacaagaata atgtagttga tatagatgct tcagccccaa agtttccccc
     1801 agtaattatt gtattttgcg attggatcag agtggagaaa aaaccttgtt cttgcgttcc
     1861 gctgactggt atacccaaaa tagctccttg tgtccaattc caagtaatat gagcaccaat
     1921 agccgtaaat aaacttcctg tactgtagat aagtgcgctg aaaaagcaac ctgctaaaaa
     1981 gatattgaaa actgccagaa atgtcatgtt aggattcaat ccgtgcaaaa gagcaaataa
     2041 tgcagcagat ccaatcattg ctattaacgg accaaatcta agcatctgat gctgaaaaaa
     2101 gccacggaat aaaagctctt ctgatgcccc cactgcaata aataaaataa aactcgttat
     2161 cagcggcaca atgtttgcgt ttcctgaatc taaccgatac gcccccgtca aatataatgg
     2221 actagtaata agcagaaaca ctccaattcc aacaacgaga cctcccgcta aataccccca
     2281 gtcttttcct cgaacagaaa caccataatc ttctattctt tttccttcga ccttactagc
     2341 aagaaataaa gctagtaaaa aaacagctaa atgtccgacc gtaccggcaa ttgctccaat
     2401 ccattcacta tttccaataa acatatccgc aagtgcatta ttgacaagaa ttcctaaaac
     2461 aaaaccaact aagactatta aaacagatcc aatcaaccga ataatcctga tcatctttca
     2521 tccctccttt atattaagtt taacaaccct tttctttctt gtctcgtgaa aactatgtaa
     2581 atgttctcca tcatagagag aatagatttt tacgaactaa gaatatctac agcaacctta
     2641 cgaaaatgaa agactgtctc cctaattctt ctgttagact gtttttaata aggaaaggat
     2701 gggaaatttt ttgaaaaaac aactactaat tatgtgcctt tcagcatgtg caattttact
     2761 attctcaggt tgtagcaagt ccaatgaaac gattgcaacg tttaaaggtg gaacacttac
     2821 agttcaggat ttttatgata acccaaaagt taaatcaaat aatcaaacac tattaaaaaa
     2881 aatgattgtc ttcaaagcat ttgatgatgt atacggaaaa gagatttctt caaaggaagt
     2941 aacaaaagaa tataatgaac aaatcaaaaa actcggtcca aactacaagg aacaattaaa
     3001 agcagttggc caaacagaag agacatataa gaaactcttt aaacagatgc tggctttcca
     3061 atacggtttg aaagccaatg taaaattaat agataaagat ctagacactg cttggaaaga
     3121 attctatcca gaagttagta cacagatcat tctattttcg actaaagaag aagcagataa
     3181 agcaaaaaaa gaagcaaatg aaggagaaaa tttctccaag cttgttcaag cttatggaaa
     3241 aaataaaaca ttaaaggaaa ctgatggaaa aatgaatttt gattcaacca atccagagat
     3301 tcctactgaa gtaaaaaaag cagcatttaa actaaaaaac ggggaagttt ctgatatcat
     3361 tcctgtaaca gatccaacga cttatcagca aagctattat ctagtaaaaa tggtaaaaaa
     3421 acaggataaa ggttctaata aagacaaata caaatctgag ctagaaaaaa ttgctaccga
     3481 agcaaaaact cttgatacgg aattcatgga taaaacaatc cgtaaagtga tgaaaaaaga
     3541 caatgtaact attaaagatc cttatgtaaa aaatatattc aaataaacaa atttgaccca
     3601 attggggatg tggcaaaatt tgagaagtga tttttggaaa ttccttctta tttttgaggc
     3661 aaaccacttt tgccacaact tcatcggggc ctttttttat ttgtaccaaa acaaatcact
     3721 ggatttgtta caatcatgag gatataattt aaaaaagttt tttatttcta tcacatgaat
     3781 caatgcgcca ttaagtagat tctttcatgc ttccctattt tttcacttgc cgtatcgctt
     3841 caaaaacaac tatcataaac tcaaactcga ctgaaaagtt attttaaaaa agactaaaaa
     3901 tatcccttct ggacttgttc ttcttattct gttatactag agaaacacga aagatatttt
     3961 tatgcgacac ttttcatttg tttttatgaa atttattcaa aactggcgta acttcagagt
     4021 tacaaggatg tattagaggt tagggaatac atcgtttaaa tgagaaataa gtaatagtta
     4081 gtaatagtca cccaaacttt aaattaatga atattgaatc gtcagatagc ctcctcgagt
     4141 tttcgagaag gctatcgttt attttatgga gaaattgatt cagaagtaat cagcgacctt
     4201 ttttagttca ccaatcgttt ctgcttggaa agtttgcacc caatcttctt ggaagcccct
     4261 cactgcttta tcaatcgagg gcatctctaa tacttgatct agtagctctt tacctaacgt
     4321 tatcgcatga gctccagatc gaatggctgt agtgacttgt tcacaatttt tgaaactagc
     4381 tgctaatatt tttgttggtc tttgcgtacg ttcgatctca gtccgcaacg aatcaatcac
     4441 tttcatagca tcaatgcctt gattttgcat cctgttataa taaggtgcaa tataatctgc
     4501 tccattatta atagctaaat acccttgtaa ttctgtgtaa atagctgtac cggtaatcaa
     4561 ataaccttct tttttcaact ttttgatcgc ttgaaggcct gcttgattca ccggcacctt
     4621 gataaacgta tcttttccca gttcttctat gataactcga gcatcctcta tcatttcttc
     4681 ttccgttctt cctattgtct gaatatggat aggaatctgt ccgattattt ttttgatttc
     4741 tttcatatgc gagaagaagt ttgacgctcc ttcttttttc agaattgtag gatttgttgt
     4801 tacgcctgtt aaattcaaaa actgttgaca agaataaata ctttttgtat ttgctgtatc
     4861 aagcatgatt tccatatagc tttcccccat tctgatcctc ttgatcgatt ttaatgattg
     4921 tcccctttct taaatgcggc atttccttat cttctacata cattgttcct ggaagttccg
     4981 gctcatgaga tccatcaaat tttatcgtga tatgtcctaa ttcattcaaa ttagtttgga
     5041 caacatctcc tactgctgtg attacataca tttgtttatc aaaacagagc ttcatatcac
     5101 aagttatttc ttttcttacc tgattcaatt caatcttata acaataatct gttaatgttt
     5161 ccggtgcttc atctccaaat aaaatcatta ttttttcttt cctgaatact tctgcttcta
     5221 taccaacatc tgttacaacc gtttcaaaga tcgtcatc
//
LOCUS       DAYYGO010000147         5199 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000147 DAYYGO010000000
VERSION     DAYYGO010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.150"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            25..696
                     /locus_tag="ACHA5T_002564"
     CDS             25..696
                     /locus_tag="ACHA5T_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFM8888093.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            755..1195
                     /locus_tag="ACHA5T_002565"
     CDS             755..1195
                     /locus_tag="ACHA5T_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888094.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1207..1875
                     /locus_tag="ACHA5T_002566"
     CDS             1207..1875
                     /locus_tag="ACHA5T_002566"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFM8888095.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1939..2562
                     /locus_tag="ACHA5T_002567"
     CDS             1939..2562
                     /locus_tag="ACHA5T_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFM8888096.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2618..3004
                     /locus_tag="ACHA5T_002568"
     CDS             2618..3004
                     /locus_tag="ACHA5T_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFM8888097.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3217..4608
                     /locus_tag="ACHA5T_002569"
     CDS             3217..4608
                     /locus_tag="ACHA5T_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFM8888098.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1728 a          861 c         1095 g         1515 t
ORIGIN      
        1 aatgatgaaa aaagggggag aaaaatgaat tttttagttg ttttaaaaga tactctgatt
       61 gagcgatcta catttctata tgttcaattg caggaaaata aaacattgct gcatttttca
      121 ccagagttcc atctagaggg gctaacatta ggagaaatct atcaagcaga agggctgtcg
      181 gctgttttgc acttttttaa ggtagaattg gaattaccag taaaagatta tatcgagctt
      241 tctcttgaat cttgggatcg agcagtggtt gaactgtttc cagaaggttt aatgatcgat
      301 acaaacgatg ggaagatcca tctgactgca gcagaactgc aaaaacaaat gcgttatcaa
      361 atagatgacg aaaattcttt ttctattttt agacgtcagc aaaagattct aaaaagcttt
      421 ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga cacagctgtt gaaaaaatat
      481 ccaggtctgc ttcacacgtc tattcaattt cctcaattga ttacgatgat ccatcgtttt
      541 atacgtatgc aacagctacc atctaagaag ctgtttcttc cattgacgga cacatttcga
      601 gtagtcaaca gagaagataa aaagaaagtg attgtgattg actttaccag aaatcggaat
      661 gtccttcacc aagttgcaat ggaaaaggcc aattaaggct ttttcttttt ggattttttt
      721 gtcataataa atattagatg atggaggaga taatatgatg caaacatggt tagaaaggct
      781 aatacgattt cagcagaact atcacaaact tatgcaggga agatatgcga aatttgatca
      841 gttgaatcgc ggtctattga tcgctgcgct tgtctttagt ttgctaagtc gttggttgcc
      901 ctatagaatc ggacaattgt tgttcatcgt ctttttcgga tggatgatat ttcgtttcct
      961 atcaaaaaaa atctacccca gactaaatga gaatcaacgt tatttgaaat atcttgatag
     1021 agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa gtcaaaatgg atcgaaaaag
     1081 ttatactttt tttgagtgcc cgaattgtca tcaaaagcag cgtgcaccta aaggaaaagg
     1141 acgaatacgt gtgacatgca aaacatgtgg tataaaattc gaaacgaatg tttaagggag
     1201 aaaagaatga aattagtatt attacgtcat ggcgaaagcg aagcaaattt tgaaaattac
     1261 tggacaggat ggctagatgt cgccttaaca gaaaaaggaa aagaacaagc agcagttgcc
     1321 ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg tttatacctc tgttttgaaa
     1381 cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg gtcagttatg gcttcctata
     1441 ataaaaacat ggcgtctaaa tgaacggcat tatggggatc ttgtgggtaa aaacaaagat
     1501 aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat ggcgcagagg atatacggaa
     1561 gttccgccgt taaccgcaga aaatagattt gaccgcagat atgatcactt ggatccgcat
     1621 ttgatcccat acggagaaag tctggaaatg acagtgaatc ggatcattcc cttatggcaa
     1681 gatcatctag caccaaaact aaaagatcgg caagatttac tagttgtggg tcatggtaat
     1741 agcatccgag cactgacaaa atatttagaa gatgttcctg aagatcaaat ggatacgatc
     1801 gatatcccaa atgcacaacc aatccagtat acattagcag atgatttgac aattctggac
     1861 aaaaaaatac tttaaagaac acctttatct aaaagtgaga gatagaaaaa agaagcaaag
     1921 gaatggaaca aaaggaacat gaaaaattat atatgggact ttgatggtac attgtttgat
     1981 acgtatcccg caatggtaga tggtgcatgg caagcactaa aagattttgg tatcagtatg
     2041 gacaaaaaag aaatctactt caaaatgaaa aagtattcta ctagttattt gatcaatgaa
     2101 tcaaacttga atgccagaga gtttaatgaa ttatttcacc gatatgaaaa agaaagtacg
     2161 gaagtttccc gtccatttcc agaaacgaaa caagtactgg aaatgctaaa agacaacggc
     2221 ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa catggggcct gttaaaagaa
     2281 catcgattgg cacatctgat tgaggaagta gtagggatcg atcaggactt tcctcgtaaa
     2341 cctgatccag catctttaaa ttatttgatc gatacttttc atttagaaag aacagacacg
     2401 atgatgatcg gcgatcgtcg tttagatatc gaagcaggga aaaatgcagg agtcgctact
     2461 tgtctttacg acatcgatca ttttctgggt gagatcccgg cagattatgt ggtaggaaat
     2521 ctgaatgaaa tccttacctt gcagagcatc attaaaaagt agtatcccct ttcaaaggag
     2581 atatgttata attttcataa aggagtgata aaattatatg gaaatcagaa gacaaaaaga
     2641 agttgtagaa gcatttcatt atgatatgag attgccgaac caagaagtag aaacagattt
     2701 gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac tatccgaaag aacatagcat
     2761 cgtaggagca cgcttagagt tccgtttagt gtttgataca tatgttttat ctggaagtgt
     2821 gagtcagatc aatcatatcc tcaatcggaa aattgaaggt caaaatgatg tcacacaaga
     2881 agaagtggac gagctagtag caccgttatt cgatatcgta cgccgaatgt cttatgaggt
     2941 gacagagatc gccttagatc agcctggggt aaaattgaat tttcaatcag aaaaacaaga
     3001 atagacagta aaaacatacc tgcgatagat taaagctatc acaggtatgt ttttttatta
     3061 ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg tctaaaatat aaaagtcact
     3121 gaaaaaaatt ttttcaggag gatggttgaa gaatcattct cgggaacctt tacttccttc
     3181 gatggacttg tcattaataa tatgaggagg agaaaaatga caacactcat cacagtttta
     3241 gtggttctag tttttgcggc attattatat gtgttctatc gtatgcaaaa gcgccataca
     3301 aaattttcaa cacgcgtctt tacagcatta ggagttggta tcgtctttgg cggtctgctt
     3361 caattgatct ttggtgtaga cgacaaagtc gttactcaat caatggaatg gatcgggatt
     3421 gtaggtcaag gatatgtatc attcttgcaa atgctagtta tgcctttagt gtttatttcg
     3481 atcattggtg cattcacaaa aatgaaagtt tctgaaaagc tagggaaaat tagtgcgact
     3541 gtattgactt ctttacttgg aacaacagct attgcagcat tgatcggaat tggttcaaca
     3601 atgctgttcg gactccaagg agctaaattc acacaaggag ctgcagaaac agcacgaatc
     3661 tctgaactgg caacacgtca ggaaacagtt gaaaacttat cgattccaca gcagatttta
     3721 tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta gtcgtgcaac aagtacgatt
     3781 ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc taggcgtgaa aagaaaagcg
     3841 ccaaaagaag cagcattctt ttcagattta gtcgacagtc tgtataaaat cattatgcgg
     3901 atcgtgacgc ttgttttgcg tttgacacct tatggagtat ttgctttgat gacaaacgtt
     3961 ttggcaacaa gtgattttgc agctatcgtc aatttaggta aatttgtcgt tgcttcttac
     4021 gtagcattgc ttgctgtttt gattgtccac tcactgattt tgattggtgc aaaagtcaat
     4081 ccaatcaact atttcaaaaa agcctttccg gtattgagct ttgcgtttac ttcacgttca
     4141 agtgctggag ctttgccatt gaatatcgag actcagacaa aagctttagg agtagatgat
     4201 gcaacagcta atttctcagc aagttttggt ttgtctattg gacaaaacgg ctgtgctggc
     4261 gtttatccag ctatgttggc aacgatcgtc gcaccagcag ctggagtgaa tgtctttagt
     4321 ttggaattta ttttgatgct tgtggcaatt gtaacaatca gttcttttgg tgtagctgga
     4381 gtcggtgggg gcgcaacatt tgcttcactg atcgttttag gggcaatgaa cttgccagta
     4441 gcaatcgttg ggttagttat ctctgttgaa ccattaatcg atatggcacg tacagcagta
     4501 aacgtcagcg gtagcatggt agctggtatc gtgacaagtg cacgcatcaa agatttagat
     4561 cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga atgcatagtc aaaaagaggt
     4621 tgtgatagaa gtggtcagct ttaagaaata agaagaagtt tcctaaaaat agtttctcaa
     4681 atttttgtga aatttcggct tatttccgag aagctacttc tgaaacaccg tgaataagag
     4741 gttgtgaaaa agctgccctg catcaagtaa taagaacagc caaacaaaaa tagttcctca
     4801 tattttacgt ttggttgagc ttattaccgc agatgcaagc ttttgaacac cgtttaagaa
     4861 agaggtcgtg agcctgccgt ttcactctga gtcacaaaga gcaatttcaa aaaatagctt
     4921 ctcatgtttg ttgaatattg tgtcttgtga ccgaggagtt gtcaggagaa caccgtttac
     4981 cagaggttgt gacttgtttt tagtcacgac ctcttttttt atttattcca tataaataca
     5041 cattatttat tccgaaaagt gaaagcgcaa ataacaaaaa caaccaactt atttattatt
     5101 aaagaaaagc gtatttaagt aggacgattc tgttgagagc tttcgtagat tttcccattt
     5161 tcctatggta aactgacaaa gttgagaatg ttagaacag
//
LOCUS       DAYYGO010000148         5187 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000148 DAYYGO010000000
VERSION     DAYYGO010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.151"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            719..1759
                     /locus_tag="ACHA5T_002570"
     CDS             719..1759
                     /locus_tag="ACHA5T_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFM8888099.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
     gene            2583..2915
                     /gene="traE"
                     /locus_tag="ACHA5T_002571"
     CDS             2583..2915
                     /gene="traE"
                     /locus_tag="ACHA5T_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFM8888100.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            3534..3875
                     /locus_tag="ACHA5T_002572"
     CDS             3534..3875
                     /locus_tag="ACHA5T_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFM8888101.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
     gene            complement(4112..>5187)
                     /locus_tag="ACHA5T_002573"
     CDS             complement(4112..>5187)
                     /locus_tag="ACHA5T_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFM8888102.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT         1792 a          719 c          956 g         1720 t
ORIGIN      
        1 tctcttatta agaactacct aatttgggta gttcttattt tttacatttt aagtagtatt
       61 taattgcaat ttggtagtat ttttttatta tcttattctt agaacggtag tacttaatat
      121 aaatataggt agtatctaat ttaagtatta atggtagtat ctaaattaac aggtagtatg
      181 taactacttt aaagtaattt ttttcagatt aacacagtag tatcttatta aataagttaa
      241 ttttaatggt agtacataat tattatatat atctgacatt ggtagtactt attgtatttt
      301 ttagtagtat ataatctatt gttttctagc ctaaagcctt gtcttataag aataatataa
      361 tatatactac tgaaataagg tagtatatat tactagtaat ggtagtatgt tttatatgca
      421 taaggtagtt cttaatttat tttttgtttt gacactggta gtacttaatt atctttaatt
      481 ttaaaactaa attttattca tacgtttttc tggtagtatt taattataaa taatatttat
      541 ttaaagtagt tcttaatgac tttaaaggta gtacataatt cattttattt ctgattttag
      601 gtagtattta attaaaaaac agttttaatg cgtttgcatt ttttatttat ctttgctatt
      661 ttcgaaaaga gtaggtaatt ttacataaat aaaacaacgt gaaagtagag gatctacaat
      721 gactgatttt aaatttttta aggcggatag agtttacaac gaattatttt atcaatttcc
      781 aaaagtcttt attgtttctg acgaatacaa aaaaatgaaa gattcaacta agattgccta
      841 tatgctttta aaagcaagat tagagatcgc aatcagcaaa cggcaaatcg atgaagaagg
      901 taatgtttat tttacttata cgacaaatga actatgtaga gtattaaact gccaaaaaca
      961 aaaagcgata gcaatcaaaa aagagttgga atcctttggt ttattattac aaaagcagat
     1021 gggatttaac aaacagttag ggaaaaataa tcctaataga ctatatctag cagaattaaa
     1081 agtctcagaa aatgatatct acttactcga aaaatttgat agagagaata gggaaaacgt
     1141 tgataaatca gagggtatga aaatcatacc caccctcgac gaaaaatcag acgctgaatc
     1201 ccttggggct caagagggta tgaaaatcat accgtgccaa aacgttgata aatcagaggg
     1261 tatgaaaatc ataccagaac ttaataataa tatattagac actaatagac acaatataga
     1321 cactgaaaaa gaccgcctac aagatcaatt gttgttagac aattttgaga caattatgac
     1381 aaacgacagc attgctacgt ttgtccctga acgagtatta aatttgataa aaacattttc
     1441 ttcaagttac agtgaagctc aaaaaaccgt ccagactatt cataatgcaa agaaaaaagc
     1501 tgaaatagaa agtgatattt cgatagtttt tgaagaactc gatagttatt atgtcaatgc
     1561 agaacaagaa ttatacacga cactgttaaa agcctatcaa aaattaaaaa ccgaaaaagt
     1621 cgaaaatatc cagaacctga tttttgtcta tgtaaaaaat tggtttatcg aaaaaccaat
     1681 agctgctaaa gtatcaagtg aaaaacgttt gaattatgca agctccccaa gcactattac
     1741 gaaagactgg ttagagtgaa actttactga caaagtgatt gttgcttcat ggagtaacat
     1801 taaaattaaa aattttttat tattctaatt atattacgaa tattacaaaa taatagctat
     1861 tttaatagaa tttataagtt aaaatattat gataaaaatc acaaacgtag ccttttacgt
     1921 cataagtgtt aaaagaaata attaatttca aatatagaaa agggaacgta tgtttgcata
     1981 ctcctttttt ttgagttata ataatacgcg aagagagata atttcattat ttaaacaagc
     2041 taaaaatctt gatattactt cttttcaggc aatacgaata ttattcgctt acattttttg
     2101 tataaattta attgtctgct atgcttgtga tgaattgatt ttcgttttgc acttttggat
     2161 gataaatttg gacaattgaa aatacaaaaa aaagaaaggc ggtatgaact cggaaggctg
     2221 gtaaccttcg tcagcgttca tacacggcat taagatttgg cccttaatgc gttaaccttt
     2281 cagtgttacg gtattacccg caatggattt agtataacag atattcaaaa gtgataccac
     2341 tttttttggc tgtgttaagg ttaaaaatac gtgatattac gatgaatcta tgcctaagca
     2401 taatgttata aaaatgtccg tgtggctctt tgccgtatga cgaacgctga aattagaggg
     2461 aacgcatgtt tggatgcgtt ccctcttttt tgtttgtcat gaacgaaaat caattctcta
     2521 tttttgaaaa ataacaagaa aaacttgtta aaaatattta aagaaattgg gtggtttatt
     2581 ttatgtcata tacaggaatt ttgagtctca aagatatttg tcactacggt aaacggtgta
     2641 ccgctacaga gaaaatcacg aagaaactat caactggcca aagtaaaacc gttgtgcagt
     2701 gtaagaaata tataatccag aaagacaaag tctcagagga aatgatttat tacattggga
     2761 aacaaaagca aataatttta aaagatccta tccccttaaa agagctatat ccaacaatca
     2821 aacatgttta tgaccaaaac ggtgtactga ttggtcgaag aaaaaatgga gtcttgcgtt
     2881 gtaccgcaaa agggatgggg cgtttgatcg gctgaattga tcattctcat aacttgtatt
     2941 ttatcgtttt tcgcttgctt gaaaatattg tcgagacttc taaaggaact tagagtcact
     3001 agaaaaataa ggagtagact tgatctatca acaattccct tcggggagtg acggggtagg
     3061 ttttaacgat tggtaacgta aaaaattggt caatcgagta aatgcgcttg tttggatagt
     3121 ggtgaaatga atttgtgtaa ataataagcg cagagtaaac aagagggaat ttagaagtta
     3181 gacaagttag aatagcgtct agattaatca tgagcagcta ctaagggatc tagactttga
     3241 cgcaaactca tggaataagg agtaggtctt ctcgtaatgg ggaggacgca caagaaattg
     3301 tgtcgtgtgc tgcagtacat gtagatactt gcgaggacgt tgattattgg atcaatcgag
     3361 catttaccgc tgtctactag ggtttctcgt ttcggacgcc ataaacgagt cgaaattttt
     3421 tatgtatttt taagtaaacg aaaaacgata aaatatgaac aagtcctgaa taaagtgaaa
     3481 aaattgatat aaaacataga gaaaatgaaa tttacgtaat ggaggaatga caaatgaaaa
     3541 attatgatcc gaatatacga ttgggcacac atactattaa ggtttctttc caacgttggg
     3601 attacaaagg atttgtgacg tttagaaggg gcggaaattg taaaggatta gatgttttag
     3661 cattagacga ggatgattta tacgatcaaa cattgactga taatccaatt ggattcggct
     3721 tgctacctga agatgatgaa ggtaatgaat ggtttaaaat gacgcttatg aatgataatg
     3781 gagacgaatt atctgttgaa gatacttggt catatttgag tgattatatt gtcagctttg
     3841 aaatcattga atttgtagcg gataaagaag aataaaaact ggaggaataa attttacaga
     3901 taaaggctta gaaggagtca cgacgattaa taaaaaggta gtattacctt aggcattgag
     3961 taaggaattt atttagtcta atggtgataa gtcaagatga aatttaagat tttctttatt
     4021 gagtaatatg ttttgaagcg cacgaaacta gacccgatca aaatcaaatt tttttcgggt
     4081 tgattcaagg atttgcgtca ataacggatt gttatttctc tatcacactc cttggtggcg
     4141 tatagagttg gtgtttacgt agtagcgtat ccaccagacg cgtaaatttt cttgcggtta
     4201 ggacgagtgc acgtttgtgt tgatgctttg gaacctcctt atatttactt tgataaaaag
     4261 ctttgtattc aggaagataa ttttttacag agttggcagc ttcaactaag tagtatctaa
     4321 aatatcgatt tccttgtttt gtcagaggtg tattttgcga ctgatagttt ccagattggt
     4381 tcactttcca acttaatcca gcgtattttg ccaattttgt ctgatcttca aatctttcga
     4441 tttgaccaat ttcagcgatt aaaccggcag catagacttt accaacgcct ggaatactgg
     4501 ttaagcattg atattctgga atgacaacaa ttacttgttc gatggcttta tctaattctt
     4561 tgatattttt ttcaagtgct cgtatttctc gtacgagaac acttaaaaca acattaattg
     4621 attcttgtgc aagagcacct aggcggtaac tcatggtaat tgcacgttga attgctttag
     4681 cgattttttc tggttgcttg aaacggcctt ttcctttttc ttgaagtaaa tcacagaaag
     4741 cttctaaagg aagctcagca agttcatcca atgtgaaatc ttccgtcatg agtgaaatga
     4801 gggttgcact aaaaagactc gtcgattcgt ctcgcatttc tcgagcaagc gtattacatt
     4861 tgtacgttag attttcaaga aaatgttgct ttgtacgaat caattgctta ataagttgat
     4921 agcgagtacg tgttaaacgc tgtaaggcca tgtatttctc ttctttgata ggggaagttg
     4981 tgaatcgttg aatacgcaag aaatcagcga ttcttaatgc atcgtttcga tcagttttat
     5041 tctcatcaaa catgcgacta aattgtttca cccgaaaagg attttcaatc gttacaagtg
     5101 tgttgagctt ctttagtttc tcatcttcat gaaaaaacat ggaaggatgg aagctgtaca
     5161 tggacgttga ctccatgcca atcacaa
//
LOCUS       DAYYGO010000149         5102 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000149 DAYYGO010000000
VERSION     DAYYGO010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.152"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACHA5T_002574"
     CDS             complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACHA5T_002574"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFM8888103.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1297..2133)
                     /locus_tag="ACHA5T_002575"
     CDS             complement(1297..2133)
                     /locus_tag="ACHA5T_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8888104.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2317..3534)
                     /locus_tag="ACHA5T_002576"
     CDS             complement(2317..3534)
                     /locus_tag="ACHA5T_002576"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM8888105.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3683..3844)
                     /locus_tag="ACHA5T_002577"
     CDS             complement(3683..3844)
                     /locus_tag="ACHA5T_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888106.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3917..4000
                     /locus_tag="ACHA5T_002578"
     tRNA            3917..4000
                     /locus_tag="ACHA5T_002578"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3951..3953,aa:Leu,seq:gag)
     gene            complement(4240..5102)
                     /locus_tag="ACHA5T_002579"
                     /pseudo
     CDS             complement(4240..5102)
                     /locus_tag="ACHA5T_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1580 a         1043 c          813 g         1666 t
ORIGIN      
        1 ttttactaga acacaaaaaa aaccttaaaa ccaaacttaa gaagtttagt tttaaggtta
       61 acttaaatta tattatgcgt ggaaacgttc cacgccatct agataagttg cttccaatgt
      121 catatcaggg ttcaatacga taaagtctgc atcgcgtcct tgagcgattt ttccacatgt
      181 atcatcgatt ttacagctga ttgctggaat cagactagcc atcatgactg cttgttctgg
      241 tgttgctaat ccccagtcta caacattttt aattgcttct ttcaatttca atatacttcc
      301 agctaagttg ccagattcta gacgtgctgt tccttctttt actactactg gaaattcgcc
      361 taaattgtaa tttccatcag gcatcccacc ggccatcata cagtctgtaa ttaatgcgac
      421 atgatcatgt cctgcttttt ccattaggat tcccgcagca tttggatgta catgatggcc
      481 atcacaaatc aattcagaaa agacatgttc taaagataat aacgcaccca ccatacctgg
      541 ttcgcgatga ttcaatccac gcataccatt gaaagcatgg acaaatacac ttgcgcctgc
      601 ttcaacagct ttttgcgctt cttctagtgt agcattactg tgtcctagag aaacaactac
      661 tccttcttca gtcacttgtt tgacaaactc ttctacacct tgacgttccg gtgctaaagc
      721 aatttttttg attaaaccac ctgaagcttc ttgccattca tggaaagtat ccaaatcagg
      781 atccccgaaa taacttgggt tttgtgcccc tttgtgttct tctgtgaaga aaggaccttc
      841 aaaatagatt cctttgattt tagctcctgg tgcttcctga tacattttgc cgattgtctc
      901 agctacatct tttaaacgtt ctttgcttga cgtcaatgtt gttggtaaaa atgaagtcac
      961 tccacaagat aacagacctt cagacatcac tttgatgcct tctgcatcat tatccattac
     1021 atcatgattc atatagccgt gaatatgtgt atctaccaaa ccaggagcaa tccatttgcc
     1081 gctttggtca atgatgttga cttctccctc aggtagttct ttggtgtatt caccaaaaag
     1141 tccatcggta atttcaagat agcctgcatg tttgacacct gacgctaaaa agaatttgtc
     1201 agcatagata aaagttctca ttctattctc tccctttgtt tttctacctc taaagtactc
     1261 ttatttagag aagaagtcaa gaatggtcta caccatttac aaattttttg aaatttgttt
     1321 cacagcttgt gctaagcctt catagaatgc tgctctttct ggttcttctt tacgcttgta
     1381 tattaagctt ttaagcacaa ataaatcatt gaggtaatag tagctattct tctcttgagc
     1441 ccaatagatt ccttgattca cctgttctaa cgcttcgtcg atttcatttt gatggaataa
     1501 gaaatttcca taattataaa aaatttgcga aaattcactt tttgatcgtt catcagctga
     1561 ttgatgcagc agttggatgc ttcgactcaa atagtagcgt gcttcagccg tttttcctgc
     1621 cacaccatat atttttccga tacaactaat cagttgtatt tctgtgtcag aaacatggat
     1681 tctttctttt tggctagagt agcttaatgc ttctttcaaa taataaaggg actgagagat
     1741 attttgacgc aacgtgaatt cgcaaacacc gagataataa taatagcgtt gaaagtcagt
     1801 tgcttgatac agattttcag aaatatcaga agattttagt acttgttcta acttccgata
     1861 atcacgtttt tggaaacaac tatccaacag ttcaaataat ttatcgtttg tccgagtagt
     1921 ttttcgagag agattcatca catgatcaat cgttacccct agccgttgac aaatctgcat
     1981 catcaccaat acattcggca gttcctcatt attttcgatc cgacttaata cactttgtga
     2041 acaaatatca tgcgaaagca ttttttgcgt cattttttgt tcttttcgaa tctctttaat
     2101 gatcgttcca aaggactcga ttaacgtttc cataagtatc ctccccaaaa tatgcgtatt
     2161 cgcattctat tcgtatttta ctttaggaag tcgcaaactt caagaaaaga cttgcaacta
     2221 aataatatac tttagttatt taagaaaact ccactagaaa gagagcctca gtgtgtttgt
     2281 ctgtggccct cctactgaaa aatttggttt ttaaaatcat ttttggcgat aggttgctag
     2341 gaattctctc attttcttag ctgtttgttt caaatcatcc attttaggaa gataaacgat
     2401 tcgaaaatga tctggctggt tccaattaaa tccaccacca tgtacaagta gaatatgatg
     2461 ttcatgtaaa aagtccaaaa cgaatttttc atcatccaaa atattgaatc gctttgtatc
     2521 aatttttggg aatatataaa aagcagcttt tggttttacg gctgaaagac caggaatatc
     2581 attgattgca ttatagatat attctctttg ttcatagatc cttccgcctg gcaggagcag
     2641 ctcatcgaca ctttgataac cgcctagcgc ggtttggata atttgctgag acaatacatt
     2701 ggaacacaaa cgcatggagg acaacatatt cagcccttct atatagcctt tgacgtgtga
     2761 cttatctcca cttaggacca tccagcctac tcgaaatcct gctacacgat gagatttgga
     2821 tagaccgttc agcgtaacaa cgaacaaatc aggcgctaat gtagcaatcg gaatatgttc
     2881 tagaccatcc atcactaagc gatcatagat ttcgtcggaa taaataatca ggttattttg
     2941 tcgagctact tcaacgattt cttctaataa ttcttttgga taaagtgccc cagtaggatt
     3001 attaggatta atgataacaa tagcttttgt ccgactcgtt atttttgatt tgatatcatc
     3061 aatatcagga taccattctg cctcttcatc gcaaatataa tgaacaggtg tgcctccagc
     3121 taatgcaata gaagccgtcc acaacggata gtccggcatt ggaaccagta cttcatctcc
     3181 attgtccagc aacccctgca tacacatcgt gatcaattcg ctcactccgt tgccggtgta
     3241 gatatcattg atcgtcacat tcggaaaatg tttcagttgg caatattgct caattgcttt
     3301 tctagcagaa aagatcccct tggaatctga gtagccttct gagtcacggg cattcatgat
     3361 caaatcacga atgacttcgt ttggtgcttc aaatccaaaa ggtgcgggat ttcccgtgtt
     3421 caattttaaa atactaatgc cctcttcttg catccgctcc gcttcttcta gaacgggacc
     3481 gcgaacatcg taactgacac cttctaattt gttggatttt tcaaattctc tcatcactta
     3541 gccaatccct catttcaaaa agttagaact ttccaaattt tttgttattt actatttcaa
     3601 tatcatactc catcctagaa aattttgcca cttatttagg aaaaacgtaa gtattaaaaa
     3661 aacacaaaca agcgttgaga acttaattgt catgatagtc attccataga ttcgctgccc
     3721 aatccattgc aattccacaa atgattccta gcataaaacc aagcaaaaaa cgtccgaatg
     3781 caagtccaat caatacacct aagatcattc ccagtcctga aaattctccg tcatatttct
     3841 ccatcttaat aagtcctccc atttttgagc ttgacaacta tgtaaaacct cgctatcatt
     3901 agtgtgttat tattacgcag atgtgttgga attggcagac aagcatgatt gagggtcatg
     3961 tgagcgttag ctcgtgtggg ttcgagtccc atcatctgca ctacaactcg tgaagttaat
     4021 tttgcttcac gagttttttt gtttgaacgt ttctttatgc cattatatac atatcactgt
     4081 atcctttaca aaaagaacgt acaatcatgt aaacagcctg aggtgagtat atgaaggtcg
     4141 gaaacaagct actcgtagaa acttgcaagt gttaaaacaa ttaatcgaaa aacgagtaaa
     4201 atccctcacc taatactaaa gcgagggatt ttttgtatat tatctgttta tcaccgttat
     4261 tataaaggta caaataccaa gcacggttaa tccaaattgc ataaattcat ttttttgtac
     4321 actcatgctt ttcattagct ctttaactgc cattagtact aagatggcga taacaacata
     4381 aagtatatag tacgtccgca ttccggaagc ccatactgtg ggagaaaatc ccataattaa
     4441 ccttgaacaa aatcccatta tcatcaaaat aactaaaaaa atagaagttt tcttattgtc
     4501 aaaacttaaa taaattccaa caattaaaca tagtaataat gccaagacta ctaaagtagc
     4561 tatccaagta cctggataaa aaagtgacag tttcgttcct aactctgtaa agagattatt
     4621 caggttacta ctactccaaa tcattgccct tttatttcca ttgacgtaag taaagctttg
     4681 acccattgta ttaccaaaat aaataatcag gtttaagaaa aacggtaatg cagtcaatat
     4741 acgcacataa tagttttgac atttcctata tgttagaagg aaaattaaga aaaatagcaa
     4801 taaaaataag atattcatgt ctaaaaaaac ggctttccaa atgatgaaat acctaaatct
     4861 aacttattaa gaaagttgaa atttttatat tcaggaaacc atttattaat ttcatgtgca
     4921 gcacgtaatg cattccctgg agttgtcaaa gagaaaatta attctgctaa gcttattatt
     4981 gaaaatactg ataacttata attatatcgt ccattgacaa tcaaatataa actaactata
     5041 cttgttagaa caaaaaaaca ggcgttgact tgttcttgat tagttaaaaa tatcaataaa
     5101 gg
//
LOCUS       DAYYGO010000150         5086 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000150 DAYYGO010000000
VERSION     DAYYGO010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.153"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(405..779)
                     /locus_tag="ACHA5T_002580"
     CDS             complement(405..779)
                     /locus_tag="ACHA5T_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8888107.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
     gene            complement(802..2202)
                     /locus_tag="ACHA5T_002581"
     CDS             complement(802..2202)
                     /locus_tag="ACHA5T_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HFM8888108.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            complement(2206..2616)
                     /locus_tag="ACHA5T_002582"
     CDS             complement(2206..2616)
                     /locus_tag="ACHA5T_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8888109.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(2634..3125)
                     /locus_tag="ACHA5T_002583"
     CDS             complement(2634..3125)
                     /locus_tag="ACHA5T_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFM8888110.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(3135..3926)
                     /locus_tag="ACHA5T_002584"
     CDS             complement(3135..3926)
                     /locus_tag="ACHA5T_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8888111.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(3919..4692)
                     /locus_tag="ACHA5T_002585"
     CDS             complement(3919..4692)
                     /locus_tag="ACHA5T_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8888112.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            complement(4848..>5086)
                     /locus_tag="ACHA5T_002586"
     CDS             complement(4848..>5086)
                     /locus_tag="ACHA5T_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8888113.1"
                     /translation="KIPSFLRINLFLHLSGMIERIMIGDGITNEHLKEGAQLFDTFLN
                     VSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
BASE COUNT         1623 a         1029 c          671 g         1763 t
ORIGIN      
        1 taggagtgag catttttcga aaaatactaa aaatttttca aaataaaaaa aggcatacag
       61 acaataaact gtctatacgc ctcacagact ctattctata catctaattt tggctgtcct
      121 gcaaaaaata tgtgttagcc tccatcgaaa gatcaaactt tggaaaacat cattataact
      181 ttgcttggta agagctttgc taaaacatga tatgacttgt atacaaaatg tggagtgtat
      241 tccaataaat aggaaggatt acaaaatttg aaaccaaata agtaaaaaat actgtcactt
      301 tgaattaatt tgaaatagta aattctcatt aaactattga aatgctgata ccaaaaaatg
      361 aagtactttt cgttcatacc aacttagagc acttcgttat ctatttattt cttcatttgg
      421 cttatctcta taatctcatg atttggtccg tagaaattaa agtaacggat tccattttcc
      481 cagaacggta attccataat atttttatct aataattcat atcctaaatc ttgaattgaa
      541 ttgaatacct catctatgtc atccgtatct aatgctagat gatcaatggc tcctctacac
      601 tcagtagttt tatcttccca tatttctatt atcgttccat tcaactcgaa aaagccgact
      661 ttacttccca cattcatttc ttcattaatc agactaaacc ctaaggattc ataaaagatt
      721 tttgaagctc caaaatcttt tgttggaatt ccaatatgtt gtaaaccatt taatttcact
      781 tacatcatcc cttcaattgt tttaaaatcc acattcataa tttattgaat agtctagttt
      841 atctaccttg attgctccct ccgtaacagt catacttaat gctgttccga ctttatcata
      901 cattctagta cttaaagcaa cattatcaat atataaaaca gctatcgtat catcaacaat
      961 taactttagt tcatattctt tgcctatttc caaaaatagt ggtcgttgca gccccttatc
     1021 aaacatttga ggccaaggat agttcggagt ttttccgaaa gtaagctgat tattaatcaa
     1081 tgaaatacga aattcgtatc cttcttgagt atcactatct acaaataatt ttaaagcaaa
     1141 ctcctttgtt aacgatgtaa tagtcaaagt tgtttcaaat agaaaataat cttcgatttt
     1201 aggaacaatt actctttctt tttttccaaa ttctttctct aacttaaaac ttgaaaatgg
     1261 agtgatttct ttaaaattct caattatttt ttcaggcagt ttaacaccaa gagttttgtc
     1321 tgtcttttga ataatttgat gtggaacaag tgcaccgccc caatcccagt tattttgatc
     1381 gttctcccct tcttttgttg ccacccagcc agttagaaaa cggccagttt cattagttcc
     1441 gactgttctt gctgcataat aggcttttcc gtcgaaagct tcgtctactg gtgatttcca
     1501 atctgaaaat aatcctgctc cgactcgata tttagtcttt ttatcttcac aatactctga
     1561 aaaaagtaaa taccacgttc catcaatttc aaatatatca ggcatttcaa tcatgttaaa
     1621 ttcattatta gcccagtagt ctcctttgaa attccaatct tttaaatctt cggattcaaa
     1681 atagaccaat ctccccgtcg gctttttctt acttgctggt tttctagctc ctagtatcaa
     1741 aacgtatttt ccatgatctt gatcaaaaat tgcaaaagga tcgcgccaat cattcttatc
     1801 atatccttct tgaggaacaa gttttgctgc ttcgccatcc tttttccaat gaattaaatc
     1861 tttagaactt gcaatcatta acacttctga agtttttcca tccaacttag ctttgcgatt
     1921 atgtccagta tacaaagcta aatactcatt gattccttgg acaatacttc cagcgtaaat
     1981 gtattgatca atgtcatcgt tgccaccacg atgaattact tccccgtatt cttcaaattt
     2041 cacaaaatca gtggttgtaa ctagtgacca cccaaaagga tcagttaaag gtttaggatt
     2101 tctcgtatct cgttgatgaa atagataaaa agttccatct ttataaaaag gcatgcaatc
     2161 cccaaaccaa aaatctttag gcttgtaaaa aattttttgc atttatcact catcctcttc
     2221 gttagtatgt ctttgcatat ctgcaagaca cgcaattgta ttcatatttc ttagattttc
     2281 gatgatatca tcgctttttt ttccttgtaa tctcaaattg acagcctcaa tcaaaattcc
     2341 taaattcatg ccaccaagaa gttgaaaccc attttgaaat acttcacttc ttttagcaaa
     2401 ttcgttgaaa ggtgttccac caatcaagtc agcaaatacc ataacttctt gattttttac
     2461 ttgagaaact attttttcaa tgttttcacg gaattcttca atgcctgcat ctccaagtgt
     2521 taccacatga caatttaaat tatcgccaaa aaacattttt aatgtatcta caaatccaat
     2581 ggcaatattt ccatgagatg taattataaa ctccatccac tcatcctctg ttcttaataa
     2641 ataaaatcct ttaaaaattc ttttgaatct ctaggagttt gtatcactgt cacttcaact
     2701 ccatcactaa ttagttcttt aaaagcctct ctctcttctt cagtaatatt tatcgtcgga
     2761 cggatgcgtt ctgttccatt ccttcttgat aaatttccta cgtttacact tgttattggt
     2821 aaaccgttat caatcaaaaa tttaacatcg attggagaaa ctgcaacaat aagaactttt
     2881 tgtccttcat atttaccaga aagaatattt ttaactgcta cttcccttgt aattattgaa
     2941 gttctaacat taggtggtgc agcgaggcga actacagctt tttttacgtc atcattggct
     3001 acttcattat taataaccat aattctgtta gcactcaatt gatttgtcca ttgtgttgct
     3061 acttgtccat gaatcattcg atcatcaata cgaacatgta taataccttt cattttttca
     3121 tccatattca acacctactt caaaatattt aacgctctta acgcaataga aactactaag
     3181 attaatagaa ttacataaac tggtttaacc tttcgtccta acaaataata cacaattgct
     3241 actacacatg caggtaataa accaggcata acttgatcaa agatttcatt tccggtcatt
     3301 gttaagtcgc cttgtttaaa ttcaaagcca acattcactt ttacaactgt ggcaatgagt
     3361 gcaccgacca ccattaaccc taaaattgat actgcatcag taatagactt taattttgtt
     3421 tcaatttgag ttagtaggct cgcacccgat tctttcccag caaggtataa aggataagca
     3481 gtaatcctta ataaaattga tgccagtatc cacaaaataa ttccaacaaa actaccttcc
     3541 aatgccatat tagctgcaat tgctccaaaa atagtccatg gaataactac aaatacacta
     3601 tctccaattc ccgctaaagg acccattaga cctgttttca gagcacttac tgcttccaaa
     3661 gacttatttt tttcttcttt ttcaattcca acattcatac caataatgta aggcgctgta
     3721 tacggcgtag tgttaaaaaa ttgtaagtga gctaaaacgg attgtttcat tccttcagga
     3781 tcatctttat atgtgtcctt gataaaagga agcatagaat acgcataacc caacccttcc
     3841 attttttcat aatttaatga agttgcacca ttgagaatcc accggaaaat tacttgtttg
     3901 tcgatttttg tcagtttctt attcatcttc gtcaaaacct cctgttgcag ctggtgctac
     3961 ttgagtatta tttgatctat ttttatatac gattagtgcg attgcagcgc caagtaacga
     4021 aatccctaaa attggtattt ggaaataaac tgccaaaacg aaaccaacta acaaataggc
     4081 aaagtaatct tttgtaggta aatatttaag tagcattcct atcccaacag caggtaatag
     4141 tccccctgct acctttaaac caccggtaac ccattcagga atataattga ttgcacttcc
     4201 cactaacgtt gggcctaata tcacagttaa caaaacagga attccagata caaagcttgt
     4261 attaattgat gccaaatatt gcatccactc taatttttga taatcttctt tttgtgcgaa
     4321 tttttctgcc ttttgttgaa aaaaaatgtt tgttgaccac tttagaacat ctagttgtac
     4381 aattaacatt gctaccggta ttgctaaagt tattcctata gaagcatcct ggttagttga
     4441 gattgctaaa taagtaccga tcaatgctgc tgtttgataa tccggtactg atgcaccacc
     4501 aaaactagta atacctagtg tcattaattg caaagtccca ccgatataaa gtccagtttg
     4561 aatatctccc ataataagtc cggtcaagaa acctgcaacg acaggttggg aggttccgat
     4621 tttgggccct tccttatccc agttaatgaa aaatccatac aacacaacta aaaatgtttg
     4681 ccaaaacgcc attttgttct ctcctttctt cttatacttt tatataagca attactgtgc
     4741 caactaacat tatccaataa agcgggaaaa tacagtaaat attaattaaa tgagcgtcta
     4801 aatatacttt ggacaccctt tcattttttt agacgctagt atgctattta gaaaatagtc
     4861 tgctcaaaaa ttgtatagat taactcatac tctcctttgg gtatttcaat acagtaaata
     4921 tctttgattt ctgaaaaaaa atcttttgat acatttaaaa aagtatcaaa tagttgcgct
     4981 ccctctttca aatgttcatt tgttattcca tcaccaatca taattcgttc aatcattcca
     5041 gaaagatgta gaaatagatt aattctcaga aaacttggga ttttga
//
LOCUS       DAYYGO010000151         5060 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000151 DAYYGO010000000
VERSION     DAYYGO010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.154"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            180..1895
                     /locus_tag="ACHA5T_002587"
     CDS             180..1895
                     /locus_tag="ACHA5T_002587"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8888114.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            1898..3661
                     /locus_tag="ACHA5T_002588"
     CDS             1898..3661
                     /locus_tag="ACHA5T_002588"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8888115.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(3701..4237)
                     /locus_tag="ACHA5T_002589"
     CDS             complement(3701..4237)
                     /locus_tag="ACHA5T_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFM8888116.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     regulatory      complement(4275..4373)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            complement(4560..4982)
                     /gene="mgsA"
                     /locus_tag="ACHA5T_002590"
     CDS             complement(4560..4982)
                     /gene="mgsA"
                     /locus_tag="ACHA5T_002590"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFM8888117.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
BASE COUNT         1506 a         1018 c          976 g         1560 t
ORIGIN      
        1 catgttttct cattttcttt tttttaaaaa aaatagtcta ttcaatttct aaagcttctt
       61 cataataaaa ctttcattta tgaaaaggct tgttctcttt tcattctaaa tttcctaagc
      121 tacaatgtgg tagtacttgt tttttcaatt gtggacaaat aaataatagg aggaatcata
      181 tgattcagtt attacgttat gcaaaagact atcgtaagca aatcattttg ggcccgttct
      241 tcaaatttct tgaagctgtc ttcgaacttg tcctgccttt aatgatggct tccttgatcg
      301 ataatggact aaaaatgaat gaccgaggaa aaatcattga aatgggctta tggatggtcg
      361 ctatgtctgt gattggtctg atttgtgcta tcatttgcca atactatgct tcagtagctt
      421 cacaaggatt tggtacagag ttacgtaatc agctaatcaa aaaaatcaat actttctctc
      481 acaacgagtt gaatcatttt ggaacagata ccttgatcac ccgaatgaca aacgacatca
      541 atcagcttca gctagcttta gcaatggtca ttcgtttatt gatcagagca ccgtttttaa
      601 gtatcgggtc agtggtcatg gcttttgtga tcgactggga agtaggtctg tttttcttag
      661 cattattgcc tattttctca atcattttat tttttatcat caaaaaaacc gttcctctct
      721 accaaaaagt gcaagaaaaa ctagatcagc tcaatgaaca cgttagtcag aatcttagcg
      781 gtgttcgtgt catccgcgca tttgcgcaga caaaaaaaga aacagagaag ttcgataaaa
      841 gcacaaatga tctagcgaac aattatttac gtgtttccaa tatctctgcc ctattatcac
      901 ctgctacaac cttgatcatg aatgtcggga tcatctgttt gctgacagtt ggcgggatca
      961 aagtaaatat tggtagcttg caacaaggac aagttcttgc tttgatcaat tatatgaatc
     1021 aaatgctttt ggcactgatc gttgtttcaa acctcgtcgt gatcttcaca cgtgccgaag
     1081 cttcaggaaa tcgggtgaaa gaggtgctag acacagaaaa ttcgattctt gaagctgagg
     1141 tcagtactac tcccgatttt tcttctgaag caattatcgc ttttgaccat gttgacttcc
     1201 gttatactcc tgattctggt ctttctctcc aaaatattac atttcagctg aagcgaaata
     1261 cagtccttgg aatcattggt ccaaccggaa gcgggaaaac gactttgaca caattgattc
     1321 cgcgtttcta tgatgtcagt tccggtacag ttataatgga cggaatggat gttcgttctt
     1381 ggcagctgga tcatctccgt cagcaaattg ctcttgttcc ccaaacttct gtactattta
     1441 ccgggactat tcgtgaaaac cttcaatggg gtaagaaaga cgccacagaa aaagaatgtt
     1501 gggaagctct gaaaatcgct caggcagaag aatttgtccg tcagcttcca aatggtttag
     1561 atacaaaagt catggaaaat gggaaaaatt tctctggcgg acaaaaacag cgactgacga
     1621 ttgcaagagc tttgattgcc aagccagcat tactgatttt agatgactct ttaagtgcgt
     1681 tggattatca aacagacttg gatttgcgta aggcgctaca gacttatctt ggcagtacag
     1741 tgattattat ctcccaacgt gtccgctcta ttcaagaagc acatcatatc ttagtgatgg
     1801 atcaaggtaa gattgctgct cagggtaccc atgaagaatt acttgctcaa tcagcagaat
     1861 atcaggaaat cgtcgcatca caggaggaag aatagttatg aaagtaaaaa aattatcctt
     1921 gcagacgctc cgccgattca ttccttacct tactgcctat gcaaaagaca tttggctagc
     1981 tgtgatacta ggaatcatca atgggattgc aacagttgtg atgacgttac aaattggtca
     2041 aagtatcgat caaatgattg gagaaggaca ggtccgcttc aatcgactgt tccatttact
     2101 tattctattc ggtggtatcg ttttaatcaa cgtagtcagt caatggctca tccaagtgtt
     2161 aagcaatcgg ttagcttact tatccgttgc aaaattaaga aaagatgcat ttgctcatct
     2221 gaatcgattg ccacttcgtt attatgatca aaactcccac ggaaatatcg tcagtcgatt
     2281 tacaaatgat atggacaata tttccattgc aatttctgcg gccttcaatc aattattttc
     2341 tggagtggca atcatcattc tttcgtttat ttttatgatg cgtctaagtc cacttctgac
     2401 attggtcgtt ctttgttcta caccaattat tttccttgtg agttggatcg ttgctcgtgc
     2461 ttcacaaaca aatttcgata gacagcaaag gattattggg gatatctcgg gctttgtgac
     2521 cgaacgaatc ggtaaccaga aaatcgtgaa agcttttcag caagaagaag ctagtcaaca
     2581 gcaatttgaa ctgctaaatg ctgatctata tgaaaaagga caacgggcac aattttcttc
     2641 ttccttgaca aacccgtctt ctcgttttgt agatcacctt gcttatttat cgattggttt
     2701 agttggcggc ctattggcat tgaagagtgg ttcgtcaatc actgttggtg tgatttccag
     2761 ctttacgatc tactcaagtc agttttctaa accatttatc gaactttctg gaatcacgac
     2821 acaactgcaa accgcttttt ctggtctaga acgagcattt gatttgatcg atcagccaga
     2881 agaaaagcct gatttgccag atgcctatgc cttggaaccc aaaaatatca aaggaaaagt
     2941 ctcttttgat catgtttctt tttcttatga ccccaagcag cgtttgatcg aagatttcaa
     3001 ttttactgct tatccaggtc aaaccattgc tatagtagga aaaacagggg ccggaaaatc
     3061 cacacttgtt aatttgctga tgcgttttta tgacacaaca aaaggaacaa ttacagtcga
     3121 tgaccatgac atccgctcta tccaacgtga tacattacgt aaaagttttg gaatggtgtt
     3181 acaagatact tggctatttg acagtacatt gcgagaaaat cttcgttttg ggaatccgga
     3241 agcaagtgac gcagaaatca atgatgccct aaaagcctct tatatgtatg aatttgtaga
     3301 acgtttgccg gaaaaattag atactaagat tggcggtcaa gggttaaaga tatcagatgg
     3361 acaacgccaa ctgttgacga ttgctcgaac gatgatcagt aatcccccta tgttgatctt
     3421 agatgaagct accagctcag tcgatacatt gacagagaaa aaaatccaac aagccttctt
     3481 agcaatgatg gaaggaaaaa ccagttttgt gattgcccat cgcctgtcca caatcaaaaa
     3541 tgcggaccag atattagtga tggatcaggg agctattgtc gaaatgggca cccatgatga
     3601 gctgcttcaa aaagatggtt attaccgacg gctctatcaa gcacaattca cagcacaata
     3661 aaaacaagca gtcttctggg atgggaagac tgcttgtttt ttatttagcg tggatcgcta
     3721 aacttcttaa aatttttttg taaggaatac tacgtcctac gtaataaatg gcaattgatt
     3781 gaatcggtaa ccatattact gtttttaaca ggcggggcgc ccaaacgacc cagctgaaca
     3841 atggcacatg gtacatcaac gcaagccata atggtgtcaa aaataagttg atgatcagcg
     3901 tgacactaag cgtagccaga atggcatttt tccaagtgat ctcttttcga tagaaaaaga
     3961 atccgtagat tgctccttca ataaatgcat tcaatgtaaa tccaataaag aatgcagatt
     4021 tcgagaagag ggccattcct actaaatcag ctaaaactgc gcctatccct gaccaaaaag
     4081 gaccaaaaag aatgcccatg atcacttgtg gaataaatgt gacactgatt ttaataaatg
     4141 gggtctcgat tgctaccagt cgggacaaag tgaccatcaa tgcaatcaat aagcccataa
     4201 tagcaatcat acgtgcgtct aaccggtgtt tcttcatttt tagcatctcc tttaagagct
     4261 gctacatcgt atcatgcagc gaatgcgaaa ataaaaccgc gaacattacg tccttcgtcc
     4321 aagaggcaac atcccatcct cttagcactt aacgctttat tttctactct gtattatttt
     4381 tgggatcttt tcccacagaa actatagcat attttcacat ttactgcaaa tacgaaaaac
     4441 taatattatt gtttatatta tccaatacgt tcgtattata tcgcatatta tcagtttgtt
     4501 tagcttttat agctacatat aggcgaacat taaacgttat ttttccatac gcttatccat
     4561 caaaatgaaa gtggatgctt gtctatttca tggacgattc ttcgaaaatc taggaagccg
     4621 gcttccagtc ctcttagtag cacctcagct gttccgatat tcgtagctaa cggaatctca
     4681 tagacatcag ataaacggat aagtgctgtt acatctggtt catggggctg tgcagccagc
     4741 gggtctcgaa gaaaaatcac caaatctaac tgatcctccg aaatcatggc tccaatttgc
     4801 tgatccccgc ctaacggtcc ggatttaaac cgatggatcg gcaagccggt tgcttccgtg
     4861 atcctctgcc cagtcgtccc ggtagcaaaa agttcatgtt tttccaatat tgttttatac
     4921 gctgttgcta atttcaccat cataggtttt tttcggtcat gagcgatcaa agcgattttc
     4981 ataagagaca ctccttttga tattgttaac atacttattt actccagtgt atcagttttt
     5041 atttcttatg gatatttatt
//
LOCUS       DAYYGO010000152         5030 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000152 DAYYGO010000000
VERSION     DAYYGO010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.155"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(91..1176)
                     /gene="xerS"
                     /locus_tag="ACHA5T_002591"
     CDS             complement(91..1176)
                     /gene="xerS"
                     /locus_tag="ACHA5T_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFM8888118.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            1525..1720
                     /locus_tag="ACHA5T_002592"
                     /pseudo
     CDS             1525..1720
                     /locus_tag="ACHA5T_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
     gene            complement(3252..3770)
                     /locus_tag="ACHA5T_002593"
     CDS             complement(3252..3770)
                     /locus_tag="ACHA5T_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFM8888119.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            4137..4385
                     /locus_tag="ACHA5T_002594"
     CDS             4137..4385
                     /locus_tag="ACHA5T_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888120.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
     gene            4888..>5030
                     /locus_tag="ACHA5T_002595"
     CDS             4888..>5030
                     /locus_tag="ACHA5T_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8888121.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQS"
BASE COUNT         1667 a          822 c          773 g         1768 t
ORIGIN      
        1 tataaaaatg ataatgaagc ttgcctttaa agttgggctt ttatctaatt ttagagacag
       61 gcttcattat tatactattt tttactacat ttacaaagcg ttcaaagcat caattgtagc
      121 tgcattgtct atatgagtat ataaagttgt tgtatttgta cttgtatgtc ccaattgttg
      181 ggcagttaat accaatgaat ttgtctgttg atagagtcta gtagccaaag tatgacgtaa
      241 tttatgtgga ctgacttgga ctctgaatcc ttttgaatat tttgctacca tccgttcgat
      301 tgcaataccg gatatacgct tggcttctcc ttgataaatt gctagaaata aagctttttc
      361 gttttctgtt actttgtatc gttctttccg aatagctaga taattagcta aatattccat
      421 tgcaaatgct gctatattta ctgcgtctct cttatttcct tttctagtta ctaccacatt
      481 attcttttct aaagataggt cctctaaatt aatatttaca acctcggaga gtcttaatcc
      541 agatcctaaa atcaatgcat tgatcgccag gtctctttct acatctcgtt gataatagat
      601 caaagctcgt ttgctcaaag tttttttata ttcattggca atataatcta gataattttg
      661 tgtatcttcg tctagaaata attttttctc gattgcgttt gcacgggcag ctaacgtagt
      721 tttatctgtg atagttgcta cttgatccat aggattattt tctaagtatg gttttcctaa
      781 aggattattg gaacttttcg aaagatattt aaatagaacc tttaatgctg tgatatttct
      841 attgatagta gaagtagata gacctaagtt attattatct ttgagcttag tttttgttgt
      901 ctcttttttt acattttcct ttgtgcgacg tttcaaatga gatttgtaca agtttagatc
      961 ataggcagtt aactgtctta tcgaggtgat tgaagtatcg ggaacgatag actcactcag
     1021 aagccattca taaaagagct tataattttt tagatattct aaagtagttc gaggtgattg
     1081 atctttatcc tcacgatctt gaacaaaatc ttttatataa tcgggcatat gaagtatgat
     1141 tgtatctatt ttttgatata tttctgtatt tttcatgtta ttcatccttt cgataagtag
     1201 gggagaggag tatctctata attggactat accaattata gagtgttttg tcaattggta
     1261 tactctgaaa tttattaatt tatttcaaaa aagcagataa ataggtatag atttaataat
     1321 ttaaaataaa tggtatacat gaataggaaa ggaaacagaa ggaggcaatc ttaatttttt
     1381 aaattgtcta aattatatag aaagaacaat ctttctatat aattagacat aagagagact
     1441 acggttgatt tgatattaac aagcgtatat gaatgtatat acaaataaaa aaggggaggg
     1501 agaagttaaa gaaaattcag agaagtgggg cactcagtag tattaacaat ttaaaaggaa
     1561 tttcttaaag aaaatgggtt gcatcctgga gatacaattt ttttagataa agaaaaaatt
     1621 caagcaacaa ttcaaaaaaa tcttaactgc tgagattgat gtagcaatat ctcaatcatc
     1681 aacaaagtac gatcctgcct ttcaaaagct tattgataaa tgtttattca ttataaaaat
     1741 aaaagaaaaa tttatgtcag cttttttcat caaagtggta atgataattc gtctattaat
     1801 tgtatttttt aggaaatatg gtataattgt tttaacgaaa aggtaattca tgatacacca
     1861 aaaaccacta gctggcgggc tagtggtttt cttttacgtt ctattcagat taacgggtag
     1921 gcttactgtc ttttttggca ataattttgc aatcaagcca tttacagatg taatgcgcgg
     1981 atacttctgc taagattgca aaaaacatat cgaaaaggta attcataata caacctcctc
     2041 cccttgccat aataggccgg ggtagcagac agcataaata ttataccata actgaaaatt
     2101 tcattttagt acatttcaat atattgatga accagcacaa taaaacatgc catgactaac
     2161 agaaaaaaac ttattaaacc agatataact tgtaaacatc caactatata gaaaacgtaa
     2221 attttctata tagttggatt aagataacga ctatttattt tatcatattt ttactatacc
     2281 ctatactttt acttggtata tttatgaata atcgcaaatt tacccctata ctactagttt
     2341 gtaagttaga ttaataaaaa tactaaagtt atttgttctt ttattaaata cacctacaac
     2401 ttataaattg atggtattta gataacacga aagaataaaa ataacagctg atagtaaagt
     2461 ttgttgacga ttgctactca atcgaaagtg agttagtgcg tatgaacttg cttctgaaaa
     2521 cttatgaaat gaaagacctt ttacatcttg cagaaatcgt tactgggatt aaactaagca
     2581 ataaaaacta atgttcccct cattctttga acttctacta tttgatttat ttattctgat
     2641 aaatgacgat ttcaccttca ggatcttcaa atcccgtata gaatttattt aagatctctt
     2701 cagtatcagt aataggatag gcgataggcc acataccctt tgatggttag aataaactgc
     2761 tcaaaatctg tatcgtatgt tggctttagc atattaacaa aggctatctc caaattataa
     2821 accataagcg ttgaatcgtt cttaacaatt tcaattcttc ctaacccaaa gtcagaagat
     2881 ataattaccg gacttgcctt acccttcatg tgggttgttg agattccata tttaaaggga
     2941 attgtcggta ctatcggcgg tctgaaaaac atcccaaaaa taaaaaaatc tttcccctgc
     3001 tagtctccta agtccctttg tagcggatat ggcaccataa tctcatttta tcgaagtgag
     3061 tcatggttta tctgttggcc aaccaaaatt gatacaatct aacgatgcac cctctacggc
     3121 attctagggg agtgcacttg cattcaccca ataaaaaccg gcagttagca aagaacttat
     3181 gtagtcgtat gttcttactg ttaacataca agaggtctcc tttccacaat tgcttagcag
     3241 taaacgggaa tttacaaagc taatctcttc cattttctta tctttgttct aaaagtccca
     3301 aatggagcaa ctgtatttac atgaataaat ttatatactt cccatacagc cgttttggtt
     3361 gcctcgtctg cccattttct tttgtgtgca gtgaatagat cttcatcact cattgaatca
     3421 atcatcatgt taatatcatc aatattacga ttgagaattt cctctaattc tttcaatggt
     3481 agatgtgcat aggtatttgt aaaccactta tataactctc ctaactgatt ccatttaaat
     3541 tgatcagaag gcgttttaac agataatcca tttctttcat cattttccca ttttagaatt
     3601 aaattcgtcc aaccaacttg atatgctaaa ttttcagcag gtgttctatc cacttcctca
     3661 actcttttat ccttcaattc ttcaggaata gtttcaaatt ctaaaatata tttagtataa
     3721 gtcctattta tttctttttt taattcttct ttatttttat atgttctcat tatattacct
     3781 ccataaattc ctaattgtct cctttaattc atctataatt tcatccacga tattttaatc
     3841 cgttctacaa tgcatatctg atatgcattg actgtctaac tcatatgtga atgatttata
     3901 aaatatccca acgtctcatc taaagaaatg ttgatcgtca gcaaggagct aaaaaatacg
     3961 aatatatttc ttttgtagta aaatcggact ggtttgcata tcattttctt cgtaaatatc
     4021 aaaatcatgt taactactat caattactaa taaaaactac cactttaagc gttaagaact
     4081 gttaagttta gttaagtgag gatagttcac aaaatccatt gttaaacttc acgaaagtgg
     4141 taaaactcaa aatgctttat ataggattac agcatcgctc tttcttcgat tgcgcgttgg
     4201 gtaaaaaata ttatgaagta aaaattgacg atgacacgtt ccttacggct catcagatga
     4261 aacagctaca aaaacgaaat gatcaactcg aagaaaattt aattctaaaa aagcgattgc
     4321 catattcacg cctcgctcga cgaactatta aaggtcaaga tttacctgaa gatcatcctt
     4381 tttaggaaat tgaagctttt tatgttgcac aatgcattta taatactaca atgatgtttt
     4441 caccagatat tcttctaggg tgcggtgtaa tgaaacagat acacttgcaa aagaaaattc
     4501 accgcgaatt tactaaaatt atgaataatt atgttaatct tccgaaattt ttttccaaaa
     4561 ttaagggatc aagctggtgt tatagattgt ttaaggttgg caaaaaaata acttcttttt
     4621 aaacaattat agaaaaccga tactctgtta agttggttga cacggtgtcg atttttatga
     4681 ctttcttatt ctgaaacttc actaacggac agtacctaaa aagtctatag tatggacgtt
     4741 tacgagtact attttatcaa aaatgctgct catttcaact tatatgtttt agtgaactac
     4801 ccccacttag cttggatagg ctacaattaa ctagacagaa aaattaaggt gtgtagacta
     4861 gaagaaaaca taccaggagg aatttttatg tctaagagaa cacgaagaac tttttcacaa
     4921 gaattcaagc aacaaatcgt caatctttac ttagctggaa agccacgtgt agaaatcatt
     4981 cgagaatatg aactaacggc ttcagcattt gacaaatggg taaagcaatc
//
LOCUS       DAYYGO010000153         4998 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000153 DAYYGO010000000
VERSION     DAYYGO010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.156"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            154..840
                     /locus_tag="ACHA5T_002596"
     CDS             154..840
                     /locus_tag="ACHA5T_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFM8888122.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            842..1786
                     /locus_tag="ACHA5T_002597"
     CDS             842..1786
                     /locus_tag="ACHA5T_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFM8888123.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            1985..2629
                     /gene="pcp"
                     /locus_tag="ACHA5T_002598"
     CDS             1985..2629
                     /gene="pcp"
                     /locus_tag="ACHA5T_002598"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFM8888124.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            2708..3985
                     /locus_tag="ACHA5T_002599"
     CDS             2708..3985
                     /locus_tag="ACHA5T_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888125.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            4243..>4998
                     /locus_tag="ACHA5T_002600"
     CDS             4243..>4998
                     /locus_tag="ACHA5T_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8888126.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEESTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQK"
BASE COUNT         1627 a          896 c         1060 g         1415 t
ORIGIN      
        1 agctttacct tacaataagt aagataattt ttgtgataaa aaaataacga aaaatattta
       61 aaaatagtgt ctatttcgtg agaaaacaac tatatttttt atgtgttttg ctttataatt
      121 aaaaaaatat tgtcatttta tggaggaaaa tatatggaaa attatcttag ccttttaggc
      181 gtcttgattg ttattctcgg gtttgcattt aaattagact cgatattgat cgtaatggtt
      241 gctttagttg ttacagcttt gactggcggt cttggcattc aaggaatgtt agaactttta
      301 ggtacaagtt ttgtaaacaa tcgcgggatg gcgattttta tcatcattat gttggcaaca
      361 ggaacgcttg aaagaaatgg tctgaaagaa tcagcggctt ccttgatcaa gcgattcaag
      421 aaagtatctg ctggtatgat tgtagatatt tatgggatct ttcgaatgat ctttgcggct
      481 tttaacgtga gttttggtgg agttgctgga tttgttcgtc caatcatttt accgatggca
      541 ttggggacca tcgaatcaaa agggttagaa gtccatccta agcatgaaga agaactaaaa
      601 ggtatggctt cagctatgga aaatgtctgc tggttctttg gccaagtact attcatcgga
      661 ggagcagggg cgttgctagt ccaatcaaca ctgaaagatc taggatatga cgtaacattg
      721 ggacagcttg ctttagtgga agttccagtg gctatcacgg ctttagttgt ggcaagtgtc
      781 tacttctatc ttaaagaaaa gaaattagcg aagaaatatt atccaggaaa ggggcaataa
      841 gatggacttt tttacaaatc ctgatatttt attaggcgat aaattactgg agatccttta
      901 tatcgtgatg ggactcgtat tgatttacac tggagtaaga aatttaacag ataaaaccaa
      961 tcctcatcgt tatgggtcag cctttttctg gacggtctta ggggtagtta ttgcaggagg
     1021 aagatggctt ccggcaatcg tgaacggtgg cttgatcttt gcgatgacca taccagcaat
     1081 tgctaagaaa gtcagcaaag gagaaagccg gcttccatca aaagaatata tggaaaaaat
     1141 gtcagacaaa ctaggaatga aaatttttat tccagctttg agtatcggtg tctttgcgat
     1201 cttgtttgct ctatttacca atttaggtgc tttagtcggc gtgggcgtcg gtgtttttgc
     1261 agccatgcta atcttgatgt tcttttctcg agataatcga ccaagtacat ttttagatga
     1321 tgcagcagat atgctgggca cagtagggcc gttaagcatg ttgccaatgc ttttagcttc
     1381 tctaggtgcg gtatttacaa gcgctggagt tgggacagtg atttccaatg cagtaggtac
     1441 gatcgttcca gaaggaaatg tgaacatcgg catcatcatt tacgctcttg gcatggtgat
     1501 atttacggta atcatgggga atgcatttgc ggccatcacc gtgatgacgg ttgggatcgg
     1561 tgcaccattc gtatttagtc atggagcgaa tccagcgtta ataggaatgg ttgcattgac
     1621 ttgcggtttt tgtgggaccc tgctgacacc gatggctgct aacttcaata tcgtgcctgt
     1681 agcgatgctg gagatgaaag acaaatacgg agtgatcaaa aaccagttgt ttattgcgtt
     1741 atttatgcta gttttccaaa ttgcttatat gattctattt aaataataga agagaggggt
     1801 tgtgacagaa taattgtcgc aacctcttct aacattgtag tggaacacta tttttttata
     1861 agaatatatt aagtttgatc tgttatgtta tttaaactat tgtttccttt atttacgctg
     1921 ttcaaggaaa atggtatgat aaaagataac catatgttaa atcaatcgga cggaggaaca
     1981 acagatgaaa gtactagtaa caggttttga tccatttggt ggggacaaag tgaatccagc
     2041 ctatgaagca gtaaaaaaaa tgccagatga aatcagcggt gctgaaatca tcaaagtgga
     2101 agtaccgacc gtattcgaaa aaagcagtca agtagtcaaa gaagccatcc aacagcacca
     2161 accagatgtc gtgatctgtg tcggacaagc aggtgggcgg tcagcagtct cgttcgaacg
     2221 tgtcgcaatc aatctagcag aagcaagaat ccctgataat gaaggaaatc agccattcga
     2281 tacggcttta gaagaaaacg gtccagccgc ttactttacc tcattgccaa tcaaagcaat
     2341 gacaaaaaat gtacaagatc atggtctacc agcgtacatc tcttatacag caggaacatt
     2401 tgtctgcaat gatatcatgt atcgtttgct tcatttgatc gaaacacaat tcccggcaat
     2461 tcgaggtggg ttcatccacg tgccattctc acccgatcaa gtgatcgatc gtccagtggg
     2521 tacgccttct atgtctttag aagacattgc ttcttctttg acttatgcag ttgaagcagc
     2581 agtagaaaat aaagaagata tttccggaaa tgccggaaca actcactaat ttagcaagaa
     2641 acactggctg gttgctaacg ttatgttagt agccagcttt ttgtaattag aaaggaaaga
     2701 aacagccgtg gaagaatggc aatccgtctt tgaagaatgg tttccaaaag aaatcagcaa
     2761 aagctatccc atcaaaattt ctaaacaata cacaagcagt caacgttggg agatctatgc
     2821 aaaattaacg aaaaaacagc gggaactagt cgataagcac cgccgatatc taatcagctc
     2881 tcgttttatg gaagaacatt atttagctgc aacagattgg gtgttttctg acttcaagat
     2941 caatccgttt tttcgaacaa aacgaagcca gcaaaaattg tattgtgagt gtggtagaga
     3001 attaaaagtg caatacatcg ttaaatcgcc aaaaaccgga aagatcctaa aactaggcat
     3061 caaccacttt gccgatcacc tgcatgtctc tccgacagtg gcagcttcga tccatcaggg
     3121 aatgacaaaa gttgatctgg ccttagacga attattatgg cttaagcaga agaatatcga
     3181 ttttcctgaa ggactatggc aaaaatactg ttttgtcttg taccaaaatc gtcggatgaa
     3241 acagccatat ttaccggata taaaattagc tcagcggctc gcagaatttc gccaagtaga
     3301 aatgccgatt tacatagcgg attatcaagc attggaaaat gaaatcaaga aaataagtga
     3361 acacatcaat gggcagtcaa aaaagcgaca gataaaaaaa gaactatttg acgactttgc
     3421 agaagaatta gtaaaggatg tagaagaatt cttgatcaac tatcgcgcat ttttacgtaa
     3481 agattggcaa tccatcgtat atgaagaagt gccagtacat cctaatgctt attttgaaac
     3541 atttatttcc gtgttgagaa agacaaaacg tcaacgaaca ccagaagtca ctgctcagat
     3601 ggaatatttt gcgaaaaacc aacgattcat ccaaccaaaa atctatctat ttatttggaa
     3661 gcaatattgt cgctatggtt ttacagaagg gttcttcgac agcattcctc ggatagtcag
     3721 aaatggtttt ttaaaagtat tgcgtaaaga acgagaagct atacaatctg ctgataagaa
     3781 agaccgtaca gtctcaaaag aaaaatggca actagtagtg aaagatatcc aatctggaaa
     3841 tgtgcaagag acaatcgaca aatggaaagg aaaacattat cgttttactg aagctcagaa
     3901 acaagcgttg gaatattatc agaagttaga agaatcactg cgtttcaatg atgaggcaag
     3961 aaagtatctg aaggaattac tataaaaaac atactaagat tagaggggaa catcattgac
     4021 gaatgatgtt ctcctctatt ttctattttt agattatagt gaagagtgaa agaaagttcg
     4081 aacaaagcct tgaggactac gagtccctat caaaaaccga acttctttac tgtcatttta
     4141 gaatcaggtt tacgtatgtt ggggccatga gcccgtgtat aggaaattgg aatcgaaatg
     4201 aagcagtaaa gttctttcaa aacttaaaaa ggaggaaaac aaatgcctta cattcttgca
     4261 ttagatgttt caatgggaca cagctacagt gtcctataca aagacgacac ctgcttattt
     4321 gaagacgaga ttgaacacac tcaaagagga tttcatgcat tactcgaaga gatccagcac
     4381 ttgcctgagc gtccattgat tgtttttgaa gcaacgggga tttattccag accagtcgaa
     4441 aagttctttc aagatcatca cttttcttac tgtttgttaa acccgttaga ggcgaaaaaa
     4501 cagatggaag aaagcacctt acgcagttgg aaaacagata aatcggatgc ccaccggctc
     4561 gcacagactc atcttaaaaa tcaacgtcag ccaaaagaag tacaaagcaa tttttactta
     4621 gagatgcgcg atttagcccg tttttatcaa gaaattgaga agaaaatcac tcgtctgcgc
     4681 atggatttac acaactgcct tcaattgact tttccagagc ttgaacagtt cttttcaaac
     4741 aggctgaccc cttacgcatt gacattgatc cgtctttttc cccatccaga ttttgtattg
     4801 gcatctactc ggacaaaaat aaaaaataaa ctgatcaacg aaacacggaa aaaaatctcc
     4861 gcgaatcgtg ccgagcaaaa ggcagatcaa atcatccact atgcacagtg tgcctatcca
     4921 gctgttgaaa aagacagtat ccactgtcaa aaaacgatct attacgccga acttctccaa
     4981 gatttactcg aacaaaaa
//
LOCUS       DAYYGO010000154         4983 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000154 DAYYGO010000000
VERSION     DAYYGO010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4983
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.157"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            98..583
                     /locus_tag="ACHA5T_002601"
     CDS             98..583
                     /locus_tag="ACHA5T_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFM8888127.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(687..1280)
                     /gene="clpP"
                     /locus_tag="ACHA5T_002602"
     CDS             complement(687..1280)
                     /gene="clpP"
                     /locus_tag="ACHA5T_002602"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFM8888128.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            1460..1987
                     /locus_tag="ACHA5T_002603"
     CDS             1460..1987
                     /locus_tag="ACHA5T_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888129.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            2078..2815
                     /locus_tag="ACHA5T_002604"
     CDS             2078..2815
                     /locus_tag="ACHA5T_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFM8888130.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            2819..3736
                     /gene="miaA"
                     /locus_tag="ACHA5T_002605"
     CDS             2819..3736
                     /gene="miaA"
                     /locus_tag="ACHA5T_002605"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFM8888131.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            3738..4967
                     /gene="hflX"
                     /locus_tag="ACHA5T_002606"
     CDS             3738..4967
                     /gene="hflX"
                     /locus_tag="ACHA5T_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFM8888132.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         1692 a          852 c         1113 g         1326 t
ORIGIN      
        1 gttttacctg ttttttttct tatgaaataa atatatgcta tgataccttt attcaatagg
       61 attagtttct acaaaaaatg aaaggagaaa aataaatatg gaacggttta tagcatcaaa
      121 tgccacagtg attggtgatg tgacattgtc agaagatgtg acgatctggt atcaagctgt
      181 tttacgcggg gacagtaatt ggataaaaat aggacaaaga acgaatatcc aagatggtac
      241 aatcatccat gtcgatcatg atgcacctgt agatattgcg gagaatgtga cggtaggaca
      301 tcagtgcatg ctacatggct gtacaatcga aaaaggggcg ctgatcggga tggggaccac
      361 gatattgaat catgcggtta ttggtgagaa tagtttgatt ggtgctggat cattagtaac
      421 agaaggaaaa gttattcctc ctaatgtctt ggcttttggt cgcccagcta aagtgatccg
      481 tcctttgaca aaagaagaaa tacaaaaaaa caaagaaaac atccagcatt atgtcgagat
      541 aggtcggatg catgcgcaag gaaaattcaa ggagcaaaga taggcagtag catccataaa
      601 ataaaaaagg atggtcagtt tgagttgacc atcctttttt atttggttta gataagagag
      661 cttaaaaaag cttcctttga aacagattaa tttaatgaag aactgttttc catgacttca
      721 tcgatcaatc catatgcttt tgcttgttca gctgtcatat agttgtcacg atctgtatct
      781 ttttcaatca cttcaagcgg ttgtccagtg cgttcagcta aaattttgtt taagcgttcg
      841 cgtgtttgca aaatatggcg tgccgcaatt tcgatttcag ttgcttgccc ttgagcgccg
      901 ccaagaggtt gatggatcat gatctcagca tttggtaaag caaatcgttt accttttgta
      961 ccagctgtca gcaagaagct tcccattgaa gcagccatac ccataacaat tgtttggaca
     1021 tctgctttga caaagttcat tgtgtcgtaa atagccatac ctgctgtaac tgaaccacca
     1081 ggagaattga tataaagata gatatctttt tcagaatctt gagcgtctaa gaaaagcaat
     1141 tgggcgatga tcgaattagc aagatcatct gttacttggc cgcttaacat gatgatacgg
     1201 tctttcaata aacgtgagta gatatcgtaa gctctttcac ctcgtgaaga ttgttcaatg
     1261 actgtaggaa ttaaattcat aatagagttc ctccttgatt tcgtttagaa atagtgtacc
     1321 ataatttagt ttctaatacc taaaatgtag tatacatttt tggtcaaaaa aggtcaaata
     1381 aaaaacttag gcaaattagt cgcataaacc cgctttatgg tatgataagc agagaaacaa
     1441 aacaaggagg aacaacagat tgatagttgg tattactggc cttatcggag tgctgacagg
     1501 tgctgtagtt gtctatatcg gcatgatact gcaaatgcgt cacttggaaa ataaagaaca
     1561 gcgtagacgt gaattggaat tgaaagtcaa agaaatcgag acgctgaatc aattaaataa
     1621 aaagatcaac gagatcttgc aaaaacggac aacgctgatg gaagaatatg tcagctttga
     1681 tgcatttgat gattgttata tcacgattga cgattttgcg tatctccaat cattttcagc
     1741 gcaaaatagt ttttatctgc caaattatat tttagacgag ctatttaaga atatctccca
     1801 tcgaaaagtg attttatctc cagatgaaac aatgaagatt ggtggatatg cttataaagg
     1861 cggacgcgtg attttagaga attttacgga tagtctgact gaaatgatca atgaaaaaaa
     1921 agctcagttg aagactatga cgcgccagcc gattggtttc ttctcgaaag atgatgtttg
     1981 gagctgatat tttgaagcat tgccgttgca catgagagcg tggtaatgct ttcttttttt
     2041 cttaatgata caatagaact gaacggagtg aagaaagatg ttacaaatct atgcccatcg
     2101 cggaagcagc ggcacccacc cagaaaatac gcttcctgca tttgcagaag ctgtacgagt
     2161 aggtgcagat ggtatcgaac tagatgtcca tctaagcaaa gatggttatt tgatcgttat
     2221 gcacgacgaa gaagtggacc ggacgactaa cggtaaaggg ctgattcgag aaaaaacatt
     2281 ggaagaactc aaaaaattga atgcaggggg caaattcagc aaagaatttc cagcagctaa
     2341 agttcctgaa ttgagagaag tgttaggact gctcctccaa aaaaattacc gagggatgct
     2401 aatgattgaa attaaaactg atcagtatga atatcccggt atcgaagaaa aattagccga
     2461 aacattaaaa agtagagatt ggccttttca acatgcatat tgcagctttc atttggattc
     2521 tttggaaaaa atcgcattac tggaaccgga gatacagctg gacttattga tgagtacttc
     2581 tgataaaaag atccaactag cagcagaaac cccttatata gaaggagtac atccaaaaaa
     2641 tacttggttg ttcgaacatc cagtccaggc agctgatttt ccaaaagcaa ttcgtgcatg
     2701 gacgattaac gatgaagaag aaatggccaa atgtcaagaa ctcaaaatag atggaataat
     2761 aactgatttt ccggagaaag ctcttcggtg gaaacaatca acgaaaagta ggtaaaatat
     2821 ggaaaaagta ttagtgatcg tcggtccgac agcagtcggt aaaacagcgc taagtgtgga
     2881 actcgcacaa ctatttggag gagagatcat cagtggtgat tcattgcaaa tctataaaaa
     2941 tctggatatc ggtacagcaa aaatcaaaga agaagagatg aataatgttc cgcatcattt
     3001 gattgatgtg atcgaaccta ctcaacgtta ctcagccgct gattttcagc attcaggtcg
     3061 acaattgatt accgagattg cgaaccgagg gcacctacct atcatagctg gtggtacggg
     3121 tctctatatc caatcccttc tatatgatta tcagttagga gcgacagaag aagcgaatcc
     3181 tgagattaga aaaaagtatg aaacgttagc tgaaacaatt ggttcccaag cattatggaa
     3241 tcagttgaaa gaaaaagacc cggcagctgc tgagaaaatc cactggaaca atcaacgcaa
     3301 agtgataaga gctctggaag ttttagagac gacaggacat agtatcacgg cacctaaaga
     3361 agagccaaag caattgtatg attacctcat gattggatta gatacgaagc gtagtattct
     3421 ttatgaacga atcaatcaaa gagtcgattt gatgctagca gaaggtttag tagcagaagc
     3481 gcgtaaggtg tatgagcttg gagaagtcca ggccagtcaa ggtattggat ataaagaatt
     3541 tttcccttat tttgaaggga aagaaacatt ggaagaagca acagaacaag tcaaactaca
     3601 ttcgagaaga tacgcgaaac gtcagttgac gtggtttcgt aaccgtttgg atgcacagtg
     3661 gtttgatctt ataagtgagc caaatcaaaa aagtcagatt gagcaaacaa tcaaaaaatg
     3721 gctggaggcg gaataaaatg gaagaaaaag tgatcatcgt aggggtagaa accgaagcaa
     3781 accagcgtta cttttcagag tcaatggaag agctagttca gctgacgaat accgcatcag
     3841 gagaagtcgt attcacaatt acacaaaaga gaccacaagt ggatcggcaa acgattatcg
     3901 gtaaagggaa attacaagaa ttagttcaac aagcggatgc gcatgaagcg gatttgatca
     3961 tctttaatca tgagctaact ccgagacaga gccaactgat taccgatgct gttggtcttc
     4021 cggtaattga tcgcgtgcag cttatcttag atatttttgc gatgcgtgct cgttcgaaag
     4081 aagggaagct gcaggttgaa cttgctcagt tagaatacct gctgcctcgt ttagtagggc
     4141 aagggaaaac attatctcgt cttggcggtg ggatcggaac aagagggcca ggtgaaacaa
     4201 agttagaaac agaccgccga catatacgga ataaaatttt tgctgttaag aaagaattga
     4261 aagaagtaga agctcatcga gagcgcaatc gccaaaaaag aaaaaatagc gagatttttc
     4321 agattggact tattggctac acgaatgccg ggaagtcaac tatcttgaat ctattgacac
     4381 aagcggatac ttattcgaaa gatcaattgt ttgcaacgtt agatccattg actaagagat
     4441 ggcgttttgc tgaaggattt gagattactg taacggatac cgtcgggttc atccaagacc
     4501 tgcctacgca actgatcgat gctttccatt ctacattaga agaaagtcaa aatatggatc
     4561 ttttgctaca tgtagtcgat gctagttctc cagatcgtat tttacaggag cagactgtct
     4621 taaaattgat ggatgaactg aatatgaaag aaatgccgat actgactgtt tacaataagg
     4681 cagaccagat cgacccagcg atgttcacgc ctacgctatt cccaaatgta ttgatttcta
     4741 ctcagacgaa agaaggtaag atggcgttga tcgaagcaat caaacgtcag ctgatggaac
     4801 taatgacccc ttatacactt tctgtgccaa gcaacgaagg acagaaatta agcgagttga
     4861 gaagacaaac aatggttttg gacgaagcgt acatggaaga aacaaacgaa tatgaagtta
     4921 aagggtttgc aaaaaccaat tcaaaatggt taagaaaaaa ccaataaaat tttatttttt
     4981 taa
//
LOCUS       DAYYGO010000155         4965 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000155 DAYYGO010000000
VERSION     DAYYGO010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4965
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.158"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..651
                     /locus_tag="ACHA5T_002607"
     CDS             <1..651
                     /locus_tag="ACHA5T_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888133.1"
                     /translation="RKDFEVVYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFL
                     QFVRTLWADGQVFLPERIYLLSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFV
                     KSLSLINSEQAGRQEIEIFVVSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELP
                     DECLCAKKRVVLCDAASYQNGLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            872..2635
                     /locus_tag="ACHA5T_002608"
     CDS             872..2635
                     /locus_tag="ACHA5T_002608"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFM8888134.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            2719..3177
                     /locus_tag="ACHA5T_002609"
     CDS             2719..3177
                     /locus_tag="ACHA5T_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFM8888135.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            3286..3969
                     /locus_tag="ACHA5T_002610"
     CDS             3286..3969
                     /locus_tag="ACHA5T_002610"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFM8888136.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            4116..4730
                     /locus_tag="ACHA5T_002611"
     CDS             4116..4730
                     /locus_tag="ACHA5T_002611"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFM8888137.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            4746..4952
                     /locus_tag="ACHA5T_002612"
     CDS             4746..4952
                     /locus_tag="ACHA5T_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888138.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
BASE COUNT         1756 a          793 c         1019 g         1397 t
ORIGIN      
        1 agaaaagatt tcgaagttgt ttataaaaat tttgttctca atgacctatc actgaatgaa
       61 ttgattttac agattattag tatcgctgaa atcgacgatg cagaaaattt tattttcaag
      121 caatcctttt tacaatttgt ccgtacgttg tgggcagatg gacaagtatt tttaccggaa
      181 aggatctatc ttctatcaga aaaagaacaa cttctgtacg gaaaaattgt agaaatattg
      241 gaagagtgga aaaagaaaaa tcaaatccaa ctaagatgga atgaaaattt cattcgaaaa
      301 ttcgtcaaaa gtctttcttt gatcaacagt gaacaagcgg ggagacagga aatagaaatt
      361 tttgttgttt cagaaagttc agtcaaacaa tttttttatc gaacccaatt tcatttgtat
      421 gcggaagaag gcatcgctga aataaatcct attttatttc gttcattaga agaattgcct
      481 gatgaatgtc tatgtgcgaa aaaaagagtg gttttgtgcg atgctgctag ctatcaaaat
      541 ggattagaaa ctgaaaagac aaagatctat ccaatctcat taaaaggtgc aaacatccat
      601 ttgtatcata ttgctcagga acttcaccta ttaaaaaaac atatttcttg atgagacctt
      661 aattaggaga ccttctacaa agctttttaa ttgtctgtag aaggttttta tttgagtcct
      721 gattcgtgcg aagatctact tatataacaa gctttctctg aaattttttt aaaatgctat
      781 tgacttagag tcaactctaa gtagtagaat agataaagtg taacaaatct attttcatta
      841 aaagaagata gtacggagga gagaatgtat tatgagtaca gtattacatg tattggatgc
      901 aatcaatagc cccgcagatg tcaaaaaatt atctgttgag gaaatgaaac agttggcaga
      961 agagatgcgc caaaaaatct tgcaaaagga cagcgatgta ggtgggcacg ttggtccgaa
     1021 tctaggtgta gtagaattaa cgattgcgtt tcattatgta tttgattcac caaaggataa
     1081 aatcgtttgg gatgtttccc atcagtctta tggtcataag cttttaactg gtcgaaaaaa
     1141 tggttttgaa aaaggacatt accgtgaagt gagcggatat tccaaccaac atgaaagtaa
     1201 acatgactat tttacagtgg ggcacacatc tacatctatc agcttagcaa caggtttagc
     1261 taaagcgaga gatctgaaga aagaatcagg aaatgtggta gcatttatcg gtgacggctc
     1321 cctaagtggt ggcttagctt ttgaaggatt gaacaatggt ggtgttttac aatccaatct
     1381 aatcgtatta gtaaacgata atgagatgtc tattgatgag aatcatggca gtttatataa
     1441 aaacttagct gagcttcgtc aaaccaacgg taaaagcagc aataacctat ttaaagcaat
     1501 gggatttgat tatcgttacg tggataatgg aaacgatctg gaaacggtga tccgtttatt
     1561 tgaagaagta aaagatattg atcatccgat tgtacttcac gtccatacgg aaaaaggtcg
     1621 cggttatcag ccggcaatcg ataataaaat gaaatatcat tggcatgtac catttgatct
     1681 gtccaccggg gaggcaaagc aagtaacttc tggagaaaca tttaatatga tcatcatgga
     1741 ttatttggat aagaaaatgc aaacaagtac agctcctatt gccgcagtca atgcagcgat
     1801 accaggcgcc tttggactga aaacattcca agaaaaacat ccagatcatt attttgacgt
     1861 ggggatcgct gaacagcact ctatcacttt cgcttctgct ttggcttctc agggagtacg
     1921 tccagtcgtc ttccaatcaa gtactttctt gcaacgggca tatgatcaat tatcacatga
     1981 tttagcgatc aacgagaatc cagtgattat tttagtcggt ggcggaacga tctcagctag
     2041 tgaccgaaca caccaaggaa catttgacat tccttactta tcaagtatcc cgaacatcaa
     2101 atatcttgct ccagcaacaa aagaggatat gatctctatg ctagattggg cgttcgaaca
     2161 aacagcgcag cctgtagcaa tccgaattcc tacacatggt gtagaacatg gtacagctat
     2221 cgcaaatgat tatagtattc cagcctatca aacagtaaat aatggaggaa aagtagcaat
     2281 tttagcttta ggcggttttc ttgaattagg aaaagaaaca gtaaaagctt ttagaaaaaa
     2341 aggaatcgaa gcaacgttga tcaatccagg aacgatcaca gaaaatgacg tagaaacatt
     2401 agataaatta aaagaaacac acgaattagt catcacatta gaagatggta gtttgtcagg
     2461 aggatttggc gaaaatatca gtcgttatta tggtgattct tcaatgaacg tattaaattt
     2521 tggtgcagag aaagaattca cggatagcgt tcctgtaggg cagctttatg aacgttatca
     2581 tttaacacca aaacaaatcg ttgaagatag tctgcgtgtc ttgaatattc aataattcat
     2641 tatgctcacc atccagataa ttgtttatac tagtatctgg atggtttttt cattataaaa
     2701 agcaaagggg tggatgttat gtatcagcaa gtagaagaat atgtcaaaaa gttccaagaa
     2761 aaatatccta gttatcaatc agaaaaatta ggtttctttt catttgggga tacagaaaaa
     2821 atggcagacg agttgagcga actagttgta aaaaagataa agactggtac atcaagtggt
     2881 tacgatctgt ataaacaaga gaatgagcct ttaccaaaag ttggtcaatt agacgtggtc
     2941 ctgaatggaa aaaatgaacc agtttgtatc atcaaaaata taaatgtgac catcttgcct
     3001 tttagtgaag tcacgaaaga gcatgcttgg aaagaaggag aaggggatcg gacattagct
     3061 tattggcgga aagtccatgc tgagtttttt aaacatgctt ataaagaagc gttgaacata
     3121 gaatttacag aagagaagct ggtcgtatac gaagaatttg aagtaatttt cttttagtaa
     3181 gaaaggtctg ctgaaagaaa tagctcatta ttgtctcctg aatagaaaag ggttacattt
     3241 ttatatgaag ctgtattttt atataacatt actggaggag atattgtgga tacactggaa
     3301 caaatcaaag gcggagtcgt cgtttcttgt caagctttgg aaaacgagcc gctacacagt
     3361 tcttttatta tggggagaat ggctttggct gcaaaagaag gcggagcagt tggtattcga
     3421 gcaaatacga cagaagatat caatgaaatc aagcgaatcg ttgatttacc ggtaataggg
     3481 attatcaaaa gagattacga tgggtcagaa gtgtttatta ctgcgacaga aaaagaagtg
     3541 gaagaacttt tggagacgca agcagaaatc attgcactgg atgcaacttc tcgtaaacgt
     3601 ccgacagaaa aaacaactgc tgaacttgtc caaatgattc acgaacacgg tcggttagct
     3661 atggcagata tttcgacttt tgaggaagcg cttcaagcgc aagaagatgg ttttgatctt
     3721 ttgtctacga ctttagcagg ctatacagaa tattcacgaa agacgcagac acctgatttt
     3781 gatttattga atcaaatcat agaacaagtc gatatcccag taattatgga aggacatact
     3841 gactctcctg aacaagtaga gaaagcatat gaattaggtg ctttttcagt cgtagttgga
     3901 tctattatca cccgtccgca attgatcact aaaagatatg tcgaagccgc aagtaaagca
     3961 aataactaaa aaacacgcac ggcacagatt cttgacaaaa aagaaaaatt gatagatagt
     4021 ttaaagcaga ttctaagaag atgaaaaaag cattactttt cattttgtct tcaagatctg
     4081 ctttttaacg aattttatag aaagaaggcg aaaccatgtt tgaacggagt ccttcagaat
     4141 tgacagaacg tgaaaattac aagttgctga ttggttcagt catccctaga ccagtagcag
     4201 tagtaacgac taaatcagca aaagatatag tgaatatcgc acccttcagt tacttcaata
     4261 tcgtcagttc gaatcgaccc atcttatcct tggcgatcca gagaaaacaa gatgcagtaa
     4321 aagacacagc aaagaatatt cttgagacaa gagaagctgt tatccatatc cttgatgagg
     4381 acaacgtaga agaagtaaat aaaacagcag ccaatctgcc taatgaggaa agcgaactgt
     4441 acctgactga cttgacttta actgattcag taatcatttc agtaccagca ttgacagaag
     4501 caaaagttcg ttttgaaaca tccttatatg aacatgtcga gatacggaat aaaaataaag
     4561 taacagctga tttatttttg ctagaagtac agaaatatca tttaaatgaa acagtctatg
     4621 atgataagac aggaaggatc gatccaagaa aattgaatgc tgtaagccgt ttggcaggaa
     4681 atgattatgc agcaatagga gaaattttca cgattgcacg tcccgaataa aaaggagcac
     4741 tagttatgtt aaaaataagc tataaaccat cgacagattc aaaagaaatg aaaaaagaat
     4801 atgaaacagt gaatgatttt ctgcaagggc agtacttaga agttccgcca ttgcaagacc
     4861 attttgtcgt tactactgtt actttagatg gtaaggaaat agaaatgcca gatcaaacga
     4921 ttagcggatt gtttaattac tttaacaaat aaatatttat tccga
//
LOCUS       DAYYGO010000156         4956 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000156 DAYYGO010000000
VERSION     DAYYGO010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.159"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(55..828)
                     /locus_tag="ACHA5T_002613"
     CDS             complement(55..828)
                     /locus_tag="ACHA5T_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8888139.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMDNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            972..1322
                     /locus_tag="ACHA5T_002614"
     CDS             972..1322
                     /locus_tag="ACHA5T_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFM8888140.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1303..2019
                     /locus_tag="ACHA5T_002615"
     CDS             1303..2019
                     /locus_tag="ACHA5T_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFM8888141.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2019..3467
                     /locus_tag="ACHA5T_002616"
     CDS             2019..3467
                     /locus_tag="ACHA5T_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8888142.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(3537..4046)
                     /locus_tag="ACHA5T_002617"
     CDS             complement(3537..4046)
                     /locus_tag="ACHA5T_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888143.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4036..4761)
                     /locus_tag="ACHA5T_002618"
     CDS             complement(4036..4761)
                     /locus_tag="ACHA5T_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888144.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHSQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         1703 a          843 c          998 g         1412 t
ORIGIN      
        1 ttaagaaaaa ataagcgatt ttcttaagaa aagaggtagc ttcaaaaaaa gatcttaaat
       61 cagatcaaaa tacttcaatg cgtggacaat tccgccttct gtatttttct ttgtgataaa
      121 ggtagaaagt tcttttaatc cgtcataggc gttgtccata gcgattttgt tgtcgcaagc
      181 ttccaacaag gcaaaatcat tcggaccatc gccaaatcca aaagtaggaa ctttttccag
      241 ctgaagttct ttttgcagac gtttgacacc tgcccctttt gagattcctt tcttcactgt
      301 atcgatcgag taaggaccat tccgataaaa agttagctca ggaaaatgtt cgtgatagta
      361 ttcgtcacct gattgagata agatcagcaa catatttact gtgttttctt taaagcctaa
      421 aggatcaatt tccggaacat ctgaatgaat gaagtcgtaa gcattgatca ttgtttggct
      481 atgaccggta caccagattt tctgatcgtt atagtaagaa agttcatgac cgttcaactg
      541 tacatgatca tacatttttt gacattcttc tgaagaaaat gtttgagaat agatttcttc
      601 gccgtctact cgaatgaaag aaccattcat ggtgaccgca gaagtaatgc ccgcatcttc
      661 catgatagag tggatttctg cttctgtacg tcctgtagcg atgacgggga gaacattgtt
      721 ttcttttaat gcgttcatag cttttgccac ttcgggtgtg atttttgatt tcgcatctag
      781 taatgttcca tcaagatcaa agaaagtgat tgcacgataa tttgacacaa ataaaactcc
      841 ttttttattg gaataagttt atcttaacaa agaatgaaca aaagacaaac ctttcgtgaa
      901 gaattcctga acatattggt tcagtcaaca atttagagta tactataaga agagaattta
      961 ggagggaaag aatgattttt ttcagtattg gtatcttatt cttaataatt ggcagtcttt
     1021 ttcggattct gccctcaaaa ggaaatctgc ctttttatgg gtatcattcg cctcttgccg
     1081 caaaaacaga cgctcattgg cggctggctc aaaagacaag cggaaactgg ttttttctta
     1141 tgggcttact gatggcattg atcggctatt atttaaagac atctgggcat acgaattatt
     1201 ttctgattga aatgcttctg ctagtatttc caattatgcc tatttttatt atgacagaaa
     1261 agaaattaca gaaatatgac ctggaaactg gagggaacga taatgaatat tttaatgatt
     1321 gaagataatc aatctgtttc tgaaatgatg cagatgtttt tcttaaatga aggttgggaa
     1381 gcaactttca agtatgatgg aaaagaaggg ctcgatgcct ttttagaaaa cccacaacat
     1441 tgggatatga ttacactaga cctgaatttg cccacactgg acggtatgct agtaggaaga
     1501 gagatccgca aagtttcaag tactgtgccg attatcatgc tgacagctag agattctgaa
     1561 agcgatcagg tgatcggctt agagatgggg gcagatgatt atgtcacaaa acctttcagt
     1621 cctctgacgc ttattgctcg aatgaaagct ttgcatcgcc gtagtgagtt ggcagaagca
     1681 agagaagaag cagcagtttc agatgatcat tttgatatcg agactagtca tttcaagatg
     1741 aatacgaaaa cacgagaagc gtatctggac aaccggccaa ttgaaggact gacgcctaaa
     1801 gagttcgatc tattgtttac tttagcaaaa aaaccacgtc aagtattctc tcgggagcag
     1861 ttgcttgaat tagtctggga ttatcagtac tttggtgatg aacggacagt ggacgcccat
     1921 atcaagaaat tacgacaaaa aatcgaaaag gtcggaccac aagtcatcca aactgtttgg
     1981 ggagtcggct acaaattcga tgattctggt gttgcctaat gcgttatttg tatcagcaac
     2041 tcttagcttt ttgcggaatg gtcgcgttga tcttattgat cgttggagtt tcttttacac
     2101 aactgacaaa acaaacgatt gaagaaaaca attaccagca actgtttgga tatgctgaat
     2161 cagttgaaaa gacaacgcaa acctatgccg atacgctccc gcagtttagt caagatcagg
     2221 cgtttcaaaa tgcattgctg ctgacagagc aagtactgac agaacaaaat gtgaacttta
     2281 tatttatcga taaaaacgaa cgtgtcattt atccgactaa tacagcttta ctgaatttta
     2341 ccttaacagc tgatcaatgg aaaaatctga ggaatggacg tcaagaaaaa ttcacatcga
     2401 ataaaaatat tttaggacaa gcacaggcga cctcttacgc acttgtgcca tttaatttga
     2461 atcatgaatt ctatggagca cttgtagtta gtcaacctgc gcgaaatatc gataacagcg
     2521 ttcgtccaat tacactgaac ctgttcaaag gatttatttt ttccagtatc gttgcagtta
     2581 ttgccagcta tgcttttgca gcattccaag ttaagcgtat caatcggtta aggaatgcga
     2641 cgaaagaagt gacgaatggt aattttgatg tacagctccc cgtacatgac aaagatgaat
     2701 ttgatgagtt agcagatgat ttcaataaaa tgacgaattc attgaaagaa tctcaagcag
     2761 aaattgaaga acaagaaaat cgtcgaagac aatttatggc agatgcttct cacgagatga
     2821 gaacacctct tacgacaatc aacggacttc tggaagggtt agaatacaat gcaatccctg
     2881 aaaatcagcg agaaaacgcg attaaattga tgaaaaatga aacagagcgt ctgatccgtt
     2941 tggtgaatga aaacttggat tatgaaaaaa tccgtacgaa tcaaatcagt atggtcatta
     3001 aaaaattcaa tggaacagaa acattgaaaa atattgttgc acagttagaa gctaaagcag
     3061 aagcagcagg agatacattg acattgaaag ccgttgatga tattgatgtt tacgctgatt
     3121 atgaccgatt tgtccaaatc atggtcaata ttattcaaaa cgcgatacag tttactgaaa
     3181 acggccaaat cacgattact ttagaaaaag gctatcttga aacgatcatc acgattgaag
     3241 atacgggaat tggaatgtct gaacaacaaa tgaaaagtat ctgggatcgt tattataaag
     3301 tagatccttc aagaaaaaat acaaaatatg gtgaatcagg attaggttta ccaatcgtgc
     3361 agcaacttgt acgcttgcat aaaggaaaat tagaagtaga aagcgaacta ggaaaaggaa
     3421 caaaattcgc tgttcgtttg cctgacgtag aaatcaaaaa agagtaaaaa acaagcggta
     3481 taaaagatca tgaatcgatc ttctataccg cttgttttta ctataaaatg attgatttag
     3541 cccgcgggct ctaaacgatc aatataagga ctttgagcaa tagtagcatc atgagtaaag
     3601 agtaagatac cttgattttc ttcatgagct aaataagaaa gcaagtggag tagctcttgg
     3661 atctccaata cactaagttc atcaaatgta tcttcaataa caatattgtt tcgcttggaa
     3721 agcaaggcat gaagtaattg cagctttact ttttcaaaag acgtcaactc agctgccgca
     3781 caattaagca aggaaggaga gatatcaagt ttttcttgat aggccaaacg atcttgtttg
     3841 atttcttttt ccggtacatc caacaggaca tggtctttaa gattcagata aggaaacatc
     3901 ggccaatttt tttggatgat cgcaaaatta tccagttttg caacaagaac ctcttttttc
     3961 aagtgctcca atattttatg ctgtttttcg agaggacaca aaataacggt caagtgttca
     4021 gcatgaatca aatattcagg tttcattacg tccaggcctc ctactttgca aatgatggat
     4081 aaaaaaagtg aaagggaata gactaagaat gagacagcat ccactgagcc caataagagt
     4141 ttgggcaaat cccttaagta tatctgtgaa aaaaagatca ttttggaaat cgatggtaaa
     4201 aaaagtctgt tgatccatcg gaattgtaag tccgcttacc gaagtcaaga tatcacggat
     4261 atctggcagt tcagtagtct ttaagacaag aaaattgatc ctttgaagaa gcgcttcata
     4321 aactgactgg aagctgacga tccacaataa aaccaagata caacaaagaa agagaggaaa
     4381 gaacagctgc cagaaaataa aaaggaataa tttggtttta gaaagaccag tcaggcgcca
     4441 attgatcaat tcctgcttgt tttgcctgac tgaatagtaa aagaaaaaga taaaaaggaa
     4501 ggcaaataga aaaaataaac tgaatatgcc ttttatataa aatcggctag ctaattctaa
     4561 agccggaaga agctctgtag atgcagaagc gccaataaat gaaagccgat cttggacttg
     4621 attggctaaa gccgattgcg aatggtaaag agtcaggcat gctatcaata aaaaagcaga
     4681 aaagcttgaa aataaacaat agagtattgt ctgctttttg taataaatta atcgagcagt
     4741 agcatagcgg attattttca tacttttcac ctctataaaa agtatagaaa atgaatatga
     4801 ataaagtatg aaattccttt agtgaaatca cgaattaaaa ccagaaaata ttttgtaaaa
     4861 agcaagaggt tgtgaagaag ctgccctatt ctattaccgt agatgcaagc ttttgaacac
     4921 tgtttattcg aaaagaggag cagcgacaat aatttt
//
LOCUS       DAYYGO010000157         4594 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000157 DAYYGO010000000
VERSION     DAYYGO010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.161"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            190..2859
                     /locus_tag="ACHA5T_002619"
     CDS             190..2859
                     /locus_tag="ACHA5T_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFM8888145.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(2950..3564)
                     /locus_tag="ACHA5T_002620"
     CDS             complement(2950..3564)
                     /locus_tag="ACHA5T_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8888146.1"
                     /translation="MINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKAMH
                     MVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPGIE
                     KRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTIIK
                     ITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            3683..4222
                     /locus_tag="ACHA5T_002621"
     CDS             3683..4222
                     /locus_tag="ACHA5T_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888147.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANSYGYGGGMYFTFTGKRQYYSVKLAMQVY
                     NTFGVGSVSCTAGGLTIGK"
     gene            4219..4557
                     /locus_tag="ACHA5T_002622"
     CDS             4219..4557
                     /locus_tag="ACHA5T_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HFM8888148.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         1423 a          790 c          860 g         1521 t
ORIGIN      
        1 aaaaattttt aagggtggga cttttgattc ctaccctttt tttgcgttta tttttgttat
       61 acgaacaaat tatgaagtta tttattctaa attttatttt tttgtgaaaa ttagactctt
      121 tttaagcaga gaaaaacaag gatatgataa actgaataca acttcataga aatttggtgg
      181 aaaataaata tgaaaaaaaa tttaaagtca ttctttagag aaaacggacg ctacgcagca
      241 gctagtttcc tgattccgtt tttgatttta gcacttgttt atctaagtat cgggatctat
      301 cctggaagca gcagaagtat tttggcaagt gatgcctttt cacagttttc taattttcat
      361 gccagcttcc gaaatgttct tcttggaaag caaagtattt tttatacatg gaatgcttct
      421 ctaggattga attatttatc tttaatttct tactatctag gcgggatctt cacgccactg
      481 gtgttctttt ttccaaacca gatgatgcca gatgcactgt atgttctgac tttgcttaag
      541 gtcggctgtg ccggattggc tttttggttt cttgcacgta cgttcaagat tcctcgctgg
      601 gggcatgttg cacttagcgt cagttacgca ttgatctcat ttatcactgc acattcggag
      661 atcatcatgt ggctggatgc atttgtttat cttccattag tgattttggg gattcatcgt
      721 gttatggatc ttaaacggcc gaaactttta tttatcagtt atttactctt gtttttatcc
      781 agtttttata tgggttttat gattggcgta ttttctttct tatattttat cgcccgtcta
      841 ttgacaaact ggtcagaata taaaaaaacg attgttcctt atggtatcac ttctttactg
      901 gcaggcggcg cttcaatgat cattgtattg ccagcagtgc tggatcttcg gtcaaatggc
      961 gaggcactga gtcagatcac tacgttgaaa acagaagcga cttcttatct ggatctaatc
     1021 atgaaaaata tgattggtgt ttatgacacg acaaaatacg gatcgattcc gttcgtttac
     1081 gcaggtctgc taccattagt cctctgtctg ttttattttg ccagtcggaa agtaccgttg
     1141 aaaaataaat ttttgtacgg cagtcttttc gccatactta tcgcaagttt ctatcttgtt
     1201 ccattgaatt tgttctggca tggtatgcat gcaccgaata tgtttttatt ccgttatgcc
     1261 tatctgttct ctttcttagt gctcctgctt gcaggaaaag gatgggaaaa gatagaacaa
     1321 gacgatcgtg ggctgttgtt aggaatcatt ttggtcgtag ctgctctctt tgcggtggca
     1381 tggggcatca aacctgaagg aagctacacc tatgtgacga tgacttcctt tgtattgaca
     1441 gtggttttct tagggattta tgcttttgct gtgggatttt atcagcataa aaaactttca
     1501 ttcaatcatc tgagtatatt gttattattg atcatgtctg ctgaagcact ggtcaatacg
     1561 aacagtatga tccatggcat tcttgatgat tggaattatg cttcccgcag tttatatact
     1621 gatccttatc ctgacttgaa agctcttgta aatgaaacaa aaaaagattc cgactcgttt
     1681 taccgcttgg aaaatctgga tccagtatct gcaaacgatt cgatcaacta tggttacagt
     1741 ggtatcagtt tgttttcttc tataagaaat cgtaattctt cctcttacct ggataaatta
     1801 ggtttccgtt caagagggac aaatttgaat atccgttata acaataatac tttgctaatg
     1861 gatgggttta caggaatcaa atataacatt tctaaaaacg aaagcacgtt cagtaaatat
     1921 ggcttccgcc aagctggtca aagtggcgac tatacactgt atcagaatgt caatgcattg
     1981 ccactagcat ttactgcacc gttgtccgtt aatgatgttg agcagccagt gacagataat
     2041 ttgaccagtc agacaaactt gatcaataca ttgtctggtt taaatgaaca gtattttaca
     2101 ttctatacac ctactttgaa gaatcagttg aatacgacga tcacaaagac aacgactggc
     2161 gtcacttact cagaaacagc agataaccag ccaaaagagc tcacttggga agtccatgtg
     2221 cctgcaaata cacaagctta cttaagtttg ttcccaacca attttgcaca attagaaagt
     2281 tctagtgcga cgatcactgt caatggtaca tcgcgaaaaa cgcaaatcaa catttctggt
     2341 caatactatg atcttggcta ttatcctcaa gagacaacag tgacatttac tgcaagtttt
     2401 tatggaacga aagaagtaag tttcatggag cctaaagtcg ttggtctaga tgttgtagcc
     2461 tatcaagccg caatgaatca gatcaaagaa aaaggtgtcg aaatggaagc cactggcaga
     2521 aaagcttctg ggactgtaaa taccgctgaa gattccatgt tggtgacaac tattccttat
     2581 gacaaaggat ggaaagctaa aattgatggg aaaccagtca aggtcagcaa tttcaaagac
     2641 gcattcttga tgataaaagt tccagcaggt aaacatactg tcactttcag ttacttgcct
     2701 gaaggattta cgataggtgc aatccttttt gtcctatgta ttgcattgtt tattctttat
     2761 gtctggtacc ttgaccgcaa tcgccccttt tatggcacga ttgatccagc aatagaagaa
     2821 aaaccgcgaa atacacacag aagaagacga agaaaataaa gaaacgatgg aatctgtgtc
     2881 actcttctat ttaaatggca aaaatttcaa tctttaaatg acaaaaacca cctgattttt
     2941 ggtggttttt tatataccta tgactaaact atctcttgct gcttcgacaa cttctacagt
     3001 gatcttatcc atttgattga ttcttaaaat ccgatcaatc tgcgcaaata aacgatgaat
     3061 aagcctaaaa tttccttttg tgatcttaat gatagttgtg atagcctcat aatcagaaaa
     3121 atcttctagc ttgatatcaa ttcctaaatc tgcccatttg tattccaaaa tatgatgtgt
     3181 ttcgtcctta ctaaggttat caaattcatg tgcaaatcct atgcgagagt aaagctgcgg
     3241 ataacgagac aatctttttt caataccagg cataccgata agaaccattg ccagatcatt
     3301 tctatcgtaa atatctctta gctgttctaa atgctgaagc tttaaacgat caatttcatc
     3361 gacaataatt aagtcaattg cttcataggt tttaggcaca ggaggatctt cttgattgta
     3421 ttttagtacc atatgcattg ctttagtcag acctattcta ctagttattg tgttaatatc
     3481 cgaactgaca cgagacatct tttcagctgg tacagtataa aaaactttat ttacacttaa
     3541 tatttcatct gttgcattta tcaagaacaa gaaaaagtct gaatattcaa aaaaataacg
     3601 gtgtacattt ttaaaaggag atgtataatc gagatttaga aagcgttttc ctcaaaattt
     3661 aatgaattag gaagtgatta ctatgaaaaa attatttagc gcacttttta ttctgttatt
     3721 tggctttatt ggtattggga cttctggaaa tatagttaaa gctagcacga tggttgaaca
     3781 aacaagtgaa atggcaaaaa cttttagtca attaacacca gaagaacaaa gttacttcaa
     3841 atccaaagga tttagtgata aagatacttt ttacactcaa aagatagagc ctcaagatcc
     3901 gaaaacacgt ttttctgcaa atgtattgcg gttaaccgca tctactaaaa aaattactaa
     3961 tacgcagggg cggacagaat atattataac aagtcccaat tcggcgatgt atcgtatttc
     4021 tacacgcttg agtttaggta gtgcaaaatc atatactcaa acagtaaatc tagctaattc
     4081 ttatggttat ggtggaggaa tgtattttac atttactggg aaaagacaat attacagtgt
     4141 aaaattagct atgcaagttt ataatacatt tggtgtaggg tcagtctctt gcactgcggg
     4201 aggattgaca ataggaaaat gataatttct tactatgtct taaatagaag tgatgatgaa
     4261 ggactcttcg tttttcaatc attacatgag gatacgatgc taaaagagtt ttataatcgg
     4321 attaagaata aacagataga aacaaattta tattttttaa gtgactctat cttgcctttt
     4381 attagcaacc ctaaaattct taacttgttg cagacaaaag atttgaaaag tttaccgatt
     4441 attgtcattg atgaggaaat tgtaaaatat ggcgcttatc taagcgtaga agaattagaa
     4501 aaattaacta acattggaat gagtattcag gaggataatg gggtacatgg aacatagaat
     4561 gaatatgcac agttgagaac ggaatttgac attt
//
LOCUS       DAYYGO010000158         4593 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000158 DAYYGO010000000
VERSION     DAYYGO010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.162"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(52..522)
                     /locus_tag="ACHA5T_002623"
     CDS             complement(52..522)
                     /locus_tag="ACHA5T_002623"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFM8888149.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(681..1139)
                     /locus_tag="ACHA5T_002624"
     CDS             complement(681..1139)
                     /locus_tag="ACHA5T_002624"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFM8888150.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1310..2455)
                     /locus_tag="ACHA5T_002625"
     CDS             complement(1310..2455)
                     /locus_tag="ACHA5T_002625"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFM8888151.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2468..3385)
                     /locus_tag="ACHA5T_002626"
     CDS             complement(2468..3385)
                     /locus_tag="ACHA5T_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFM8888152.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3813..4376
                     /gene="efp"
                     /locus_tag="ACHA5T_002627"
     CDS             3813..4376
                     /gene="efp"
                     /locus_tag="ACHA5T_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFM8888153.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT         1436 a          838 c          871 g         1448 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tcttttttta tttttattaa gttaaagaac
       61 ggattcaatt gcagcggtca ttttcaatgg agaagtttgt ggtgcaaatc gttcgattac
      121 tttgccattt ctatcaatca aaaatttagt aaaattccat tttattgctt tagtgacagt
      181 accaggagct tcatttttaa gataagtaaa aagaggatct gtattttctc catttacgtc
      241 aatcaattga tgcataggga aggtaacgcc ataagtggtt ttacaagctt ctgctgcttc
      301 agtcgctgaa tctagctctt gtttaaattg attggaagga aagcctaaga taaccagacc
      361 ttgtgtctga taagcttggt aaagttcttc taattgttta aattgaggtg ctagaccgca
      421 ttttgttgca gtgttaacaa tgattatcgg tttgcctgca tattttttca atgaatagac
      481 agtgccgtct tctaaagtag tttcaaaatt ataaatagac ataaagtttt ctcctttcta
      541 ttttatataa taaaaagagc gctaaactga aaactagcta tttgttttcc cgtttagaac
      601 tcttttcaga caataaatat ttaataaaat acgaagataa gatagctata ttgcttctca
      661 tctttacgtt cttttagaaa ctatgcttct acttcagacc attcatcgcg ttttgctaaa
      721 aattcacgag ccaaagcttg tgcaccttct agtgtatggc tggcagccca gccacattgt
      781 gtttcattgc ttgcaggtac ttcagtagct tcaagaacat ctttcattgt tgcttcaaga
      841 acatctaaaa tgtcttcgta gctttcatga ttcaatacag tcaagtaaaa gcctgtttgg
      901 catcccattg gagaccagtc tacgatgtaa tctgcgtgat tgcggattag ttcggcagtc
      961 aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt ctttatttgg ttgtttaaag
     1021 cggacgtcgt atttaatgat gacatcgcca tgcgcacccg tttttcgatc agctacacgt
     1081 acataaggtg ctttgacttt tgtatggtca agattaaaac tttcgacatt cattctcatt
     1141 tgtaagactc ctatctgttt gttcattcgt tttgatgctt ttcactaaag aattatttag
     1201 tgaaatttac aaaaacgtct ttctacttga ttctaacact tatcgtttcc aaggtgtagc
     1261 aatctgaaat attttttaga tttttctgct aagtcgttta ttgggacttc tagttcttga
     1321 ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg accatactca attcctacag
     1381 aaagacggat caagccgtcc tttatgccag cctcttctcg tttttctttt ggaatagacg
     1441 catgagtcat gacagaagga atttccacta aactttctac gccacctaaa ctttctgcta
     1501 aagtaaatag ttggagtgat tcaacaaatg gaatagcaga ttcttcattt tttaaagtaa
     1561 aagaaatcat tccgctaaag ccgctcatct gctgcttagc aactccatgg agttcatgat
     1621 cgggtaatcc tggatagtag accttttcta ctgctggatg agaaaataga aaatcagcta
     1681 ccacaaaagc atttttttga tgttcctcca tacgaacact tagtgtcttg attccgcgct
     1741 gaagtaacca gctatcttgg ggaccaagta cggctccgat agcattttgg taaaatccga
     1801 tctgatcggc tagatcttta tggttagaag tcactaaacc agctactaca tcgctgtgtc
     1861 cgcctagata tttggtaccg ctatggacaa cgatatcaga accgagatct aaaggtcttt
     1921 gaaaataagg agtagcaaat gtattgtcag caataactat caatccatgt tgatgagcca
     1981 gagtggcact tttttctaaa tcagtaatct ttagaagcgg attgctaggt gtttcaagat
     2041 aaagtgcttt tgtattgggt ttgatggatt gttctatttt atcaagatta ctcgtatcta
     2101 tgatcgtgta ctcgagacca tttttagtaa gaactttgtc aaatagacgg aatgttcctc
     2161 catagacatc atctcctaat aagatatgat cgccagcttg aaaaagagaa aaaacggcat
     2221 gaatcccaga aagaccagaa gaaaaagcga atcctcgaac accgccttcc aaatcagcaa
     2281 tcagttcttc caatgcaaaa cgagtaggat taccagatct tgaatattca tattgtttag
     2341 gctggccgac gccatcttgc cgataagtgg atgtttgata aataggtaca ctgacagctc
     2401 ctgttgtggg atcttcactg atgcctccgt ggatcaattt tgtttgaatg tgcatgtttc
     2461 tttcctccta taagtaaatg ttttttgata aatagcggtc tgctgcgtct ggaaaaatcg
     2521 taacgacacg atgaccaggt ggtaaacgac gaatttctct taatgctgct gcaaaagccg
     2581 caccactcga acttccaact aatagtcctt gttctcgtgc taactgccta gtatagttaa
     2641 acccttctac atcagagatt gtctcgatct gattgattga aaggggagaa agaaacggcg
     2701 gaatgaattc tacaccgata ccctcaattt cgtgaggggc tggatcacca ccatttaaca
     2761 cagagccctc tggttctaca ccgattattc gaatgtcggg atatttttct ttcaaaaagg
     2821 tagaagttcc agcaaaggtt ccaccactcc caattcctgc tacaaaacta tggatatttt
     2881 ctttgatttc atgaaagatt tctggcccta acgtatgata gtaagcagca ggattatctt
     2941 tattttcaaa ttgaagaggc agatagctgt ttgatatctc ctctgcaagt tttttacttt
     3001 tgctgatggc accgatgatt ccttgctcac taggagaatg gatgatcttt gctcccaatg
     3061 ctttcatcaa ttgttgtttt tctaagctaa acttctctgg aacaacaaaa accgttctta
     3121 atttgtatgt gagtgcggcc agagcgatcc caattcctgt atttccggca gttggttcga
     3181 tgatcgtcgt tttttcattg atttttcctt gggcaaatcc ttctttgatc aaataactgc
     3241 ccaatctatc tttgatactt cctccaggat ttaaatattc tagttttgca taaatagcag
     3301 aacctttagg catagaatag tgctgattgt caaagcgata aagcggtgtg gaaccaatag
     3361 cttctgtaat tgtcgagata atcatgtggg cctcctaaaa aaagataata aaaaaaggag
     3421 ctgactatta aatcagctcc ttgaaagaca tagagaaaaa ttgagtagat aaaaagataa
     3481 gttacctggt actcattcta tgaagggcaa ttaatagaca tgacaaatag tatgctgctt
     3541 ttatgcacca tacaacagca acgattcaat tgtttttggt tgctgactgt catgtgtatc
     3601 cctccaagta aaatttttat taacgatagc atgaaaaaaa ataaaagtca aaggcttgaa
     3661 agaaaaaaat atttcgatag catcgtttac ccgtgtttat ttttctgaca aataggcatg
     3721 agaccttaaa agatagagaa tactctagtt ttctggccgg ttgtctgata caataaagtt
     3781 gattatgata aagaaaagaa ggagcaaaaa taatgatctc agcaagtgat ttaagagccg
     3841 gtatgacttt cgtacaagac ggcaaactga ttaaagttct ggatgcaagc caccataaac
     3901 ctggtaaagg aaatacagtt atgcgtatga aactgaaaga tgtccgttca ggtgcaacat
     3961 acgacacaac gttcagacca gaagaaaaat ttgaaaaagc acatatcgac acaaaacctg
     4021 tacaatattt gtacacgatg gatgatacag ctttcttcat ggatttggaa acatatgagc
     4081 aatatgaaat tcctgtagaa acagtagaag aagaaatgaa atatatccta gaaaacatgg
     4141 aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt tcaattacca acaactgtcg
     4201 tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc aacagtcact ggttcaggta
     4261 agccagcgac aatggaaact ggcttagtag tcaatgttcc tgacttcatc gaaacagatg
     4321 aattgttgga aatcaataca gcagaaggtt cttatgtaaa acgtgcgtca aaataattcg
     4381 ctaacggatt gataagaatt tgaaaacaga gcttgacctc ttgaaaggca gatcatcgga
     4441 tctgcctttc aagaggtttt ttgtggaatc agcaaattga aaaataaaaa agtatttgat
     4501 tttatttata ttttgaaaga tgaatggcaa aagctagaga aagaaaaaac tgcacttatt
     4561 tatgaagaaa ttgaaatgat tcataaaatt gaa
//
LOCUS       DAYYGO010000159         4513 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000159 DAYYGO010000000
VERSION     DAYYGO010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.163"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(39..284)
                     /locus_tag="ACHA5T_002628"
     CDS             complement(39..284)
                     /locus_tag="ACHA5T_002628"
                     /inference="COORDINATES: protein motif:HMM:NF024059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8888154.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(278..679)
                     /locus_tag="ACHA5T_002629"
     CDS             complement(278..679)
                     /locus_tag="ACHA5T_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8888155.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(866..2674)
                     /gene="uidA"
                     /locus_tag="ACHA5T_002630"
     CDS             complement(866..2674)
                     /gene="uidA"
                     /locus_tag="ACHA5T_002630"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFM8888156.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(2697..4463)
                     /locus_tag="ACHA5T_002631"
     CDS             complement(2697..4463)
                     /locus_tag="ACHA5T_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFM8888157.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
BASE COUNT         1413 a          893 c          570 g         1637 t
ORIGIN      
        1 tcttttaaac agaaaaagtg gaaaggggat ttttttaatt agttaatttt aaattttaag
       61 atcgcttcta ttaatttaat ttctagctga cttatcatat attcatctac ataggtaacg
      121 aattgaccta cctcgttaaa tccatttgtc aaacataact tagccatata agaatgataa
      181 tgtttgagta tcatcttgat cgaaattgga tctccttcta gagcagataa gataatggat
      241 agtggaacta ataggtactg ccgcttttta gatttatcta ccataggtca taccccattt
      301 tcaattgttc aactgccttc tttcgttgat accagatacc acttgtgctc atattgagaa
      361 ttttaccgat ttcagcatca ttaaaacctg caaaataata aagtaggata atctttcttc
      421 ttatctccga taactgatca attgctgctg ataacttttg atctgaaatt aatatttcca
      481 ttccaaataa gaaatagaca tcttgttcat caaaatgagt ttgggaatac ctgccttcat
      541 ttgaatctgc caatggttcc tcattatcct ttatgttagc gtttcgtttt cgaatagact
      601 tagcttcgtt tcgtaatact tttttacagt aggcatcaaa ttggtgttgt atagatatgt
      661 cattgtaatg ttgttgcatg ataatatgct cctttttccc cctttccact aatacaacaa
      721 tcagaaagcc cctttttttc gaaaaaacta gaaaaaaaac ataaaaatat ttatcattct
      781 ttaaacagaa aatctttcta taaaaaacaa aaaaaaacga ttaggctagg ctaatcgttt
      841 tttgaacatt cttaaaaaat ccaaattatt ttttataact gaaattgggt attgatgacc
      901 atcgtttctg tagatagtaa gcaatcattt taggttttct atctctagta aagattcctt
      961 tcttatttcc ttgcactctt aaaatccctt gacttgttgc aaaatctgca aaattccaca
     1021 cttgttcacc aacaaaattt tcacatgtat ctattacttc gtgatttgct tcgtaataag
     1081 ccacttgata ctcttctgta aacattgttg gtactgtatc atgaaatcca gctactgtat
     1141 ctgctccata ttctgtaaac attattggcg tatcaggaca tctttccatc catccatcta
     1201 gctctttttt caacagatat tttgcatgtg ccagatcccc tcccgaaaca taccaaccat
     1261 aataacgatt taaacataaa acatcgacaa tatctcctac cttacaaaca tctggcgttg
     1321 attttagata agtaacgatt gttacaggtc ttttctgcgg atcaagttct ttagttaatt
     1381 caactaaagg ttcaaaatat tctttagcac cttcggaatc cgaatctggt tcatttgcaa
     1441 tagaccacat tacaacactt gggtggttct tgtcgcgttc tatcaattcc ttgatcaccg
     1501 ttttatgaga attttctgtt cgtatctctt tccacgtatt atgtttttcc acattatcat
     1561 ctaataaagt ggccatgaaa tttaaatgta accctactgc aggtacttca tcaataacta
     1621 caataccttc tcgatctgct aatcgcataa tttcttctga gtaaggataa tgtgatgttc
     1681 taaaagaatt agcacctatc catttcatta aattaaaatc tttaatattt acagcctcat
     1741 tcaaaccacg tccattaaca tacgaatcct catgcttacc aaatccttta aaataaaaag
     1801 gtttattatt aatcaaaaat ttgccattgg taacctgaac actgcgaata ccaaattctt
     1861 ctgtataggt atcgatcact ttattatcat caattaaact aattttcata ctatataaat
     1921 aagcgtctag aggttcccaa aaaattggat tcttaagctg aatcattcct ttctttcctt
     1981 cagcttcagc tacaagttga gcctcttcgt catatatcgc tattaagatt tttccggtcc
     2041 attctgtatc gatattgaat tctacacttg catccaaatc agttattctt gtattaatag
     2101 taatatcact tatatagtct ttcggagttg tatataactt aacaggtcta tgaatacctg
     2161 cataattgaa gaaatcaaaa ttaactaagt tttccttttt tatatcccca ttttcctgta
     2221 ctgtttcctt cataatcccc acaggtaaag tactatcgtc aataatattg tttacagcaa
     2281 ctgtaattcg attattcccg tcttttacaa accgattaat ttcagactca aatggtgtaa
     2341 acccaccttt gtgttgagta ataaactttc cattcaaaaa aacttttgct tcatgtgttg
     2401 cagatccaaa acgaagaact aatctttctt ctaataatct tttaggaaga gtcacttctt
     2461 tttcatacca cacccatcca acatgatttc taatgttctc tgtttcaaat aaatcattat
     2521 aagaagaggg aactgaaatc ggataactct ctttttcatt tattggttct aaagcaagtt
     2581 cttcctgtaa gtcatcattt gtatctaatt taaatctcca gagaccgctt aagtcaatta
     2641 cttgcctcgt ctctgtcata atgggataca acatataaac cactcctatc taaatattaa
     2701 ataaatgtat cctttaaacc acaataagca tctattggac ttactccctt aaattcagaa
     2761 agacccatta gtttgtccac caatatttct aaagtttctt gttttccatc ataagtatta
     2821 atataagtct ttacttgagg tacatcagcc aaatgaaacg gacactgtac agatacaaaa
     2881 attgttggaa tttcatgaat gtaaaacggt atatccggag ttcctttact tgctggccag
     2941 acaattcgtt gaactgtgcc tggattaaca ttagctatat taatgatcaa gtcatatttt
     3001 ttagttaaat ttttaattgg tctcttagca gcatatattc cagctatatt tttaattttt
     3061 tcttcttgcg ataatccttt caattcttct tcaggtgaaa tgtatttttc aacttcaaat
     3121 ccttttgctg acaaaatctg tttcaataaa tcaatggctt gaggtctatt tccagcaact
     3181 acttcaccaa acccaccttt taccccatgt acatctacta gtaaaatcct tttatatttt
     3241 tctggtacaa taggccaaat atcttgttta tctttaacta atgtaattgc ttggtcagcc
     3301 acctctttaa aaattgattt attttcaatt aaaccaattt tttccagagc ttcttgctta
     3361 ggtaataaaa tttgttcttt tgcttttttg tgtaatccca atttagcttt taaccctaaa
     3421 attcttttta aagcatctaa taaccgatca tcagtgataa ttcctttacg atagccttcc
     3481 atcatataat taaagtcttc atctggatca ttaaaaaata gaaatagatc acatccagct
     3541 gcaatagaag tgggcaataa ttctgatcgt ttcattgccc cagtcattcc taacatatga
     3601 ctagcatcag taactactaa tccattaaat ccgagttttc ctctaagtaa gtcagtgatt
     3661 aattcttttg ataaagttgc aggaagtaaa tcttcatctt tcagatcagg attaaaatgt
     3721 tttgaataag ctggcatatg gatatgacct gccataattg aaggtaatcc agcttctatt
     3781 aagcctgagt atacttttcc aaaagaatta tcccattctt ccgtacctaa actattaata
     3841 ctaaaagata gatgctgatc tcgttcgtca ataccgtcac cagggaaatg tttagctgca
     3901 ggcaaaattc cactctcctg aatacctttc atataagcta aactaagttc taaaacttta
     3961 tcagggtttt gtccaaaaga tcgactagaa atgataggat ttctccaatt tctattaata
     4021 tctacaatag gagcaaaact ccagttacat ccaatagccg atgcttcaat cccagaaact
     4081 ctccctaatt cataagcata tttcgcatca tttgttgcac caatttttac ttctacacca
     4141 acttcagtac catctgtaca agctccgtta ccacctgctt ccgtattagc agcaattaat
     4201 agaggaattt tactttctgt ttgtaagata tagttttgat catatacctc atcagcagtt
     4261 cctggattat atcgtacagc tccaatatgg tactgattga ccatattact caagtaattt
     4321 ttatcacgac tagatcccat attaacaaac aattgaccta ttttttcttc caaagtcata
     4381 gaatcaatcg tctcttcaat ccaagaaata gcatcttcat ctaaattata tggttttttt
     4441 cgtaaatcta cttttatagt cattttcttt ctcctctact tattagtaag atttttccaa
     4501 aaagtatcta tat
//
LOCUS       DAYYGO010000160         4486 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000160 DAYYGO010000000
VERSION     DAYYGO010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.164"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(28..963)
                     /gene="murB"
                     /locus_tag="ACHA5T_002632"
     CDS             complement(28..963)
                     /gene="murB"
                     /locus_tag="ACHA5T_002632"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFM8888158.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1036..1956)
                     /locus_tag="ACHA5T_002633"
     CDS             complement(1036..1956)
                     /locus_tag="ACHA5T_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8888159.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2069..2821
                     /locus_tag="ACHA5T_002634"
     CDS             2069..2821
                     /locus_tag="ACHA5T_002634"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFM8888160.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            3685..4218
                     /locus_tag="ACHA5T_002635"
     CDS             3685..4218
                     /locus_tag="ACHA5T_002635"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFM8888161.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT         1323 a          878 c          795 g         1490 t
ORIGIN      
        1 tttacttatt ttaagttcca ttacttatta attggctaaa cttctgcttg tttctgatat
       61 tgcttgtttt tctccaatga tccgcacttc tgtttctaaa gacacatcaa atttttcttt
      121 gattacttct tggatgtgcg caatcaattc aatataatct gtcgctgtcg catgatcgat
      181 attgacgatg aatccagcat gtttttcaga gacttgtgca ccgccccatt tcaggccttg
      241 caatcctgct tgttggatca gctgacctgt atagtgtcct actgggcgct taaagacact
      301 tccgcatgaa gggtattcta atggctgttt tgattgacgc aattcggtca actcatccat
      361 tcttgctttg attgcttcat aatcccctgt ttgcaaactg aatcgagctt ccaatacgat
      421 agcacgtaat tcttggactt tactgtgacg ataagaaaat accatttcgt cttttgtcaa
      481 agttttcaat gttccatctt caagcaaaag atccacttca gcaaaaacat ctttgatctc
      541 tccgccatac gcacctgcat tcatataaac cgctccaccg acacttcccg ggatgccgca
      601 agcaaattca aatccagtca atgattctgc caaagctgca taagtcgtat caatcagtga
      661 ggcacctgct tccgcaatga ccatcgttcc tttgacatga atctgtttca tttcagtcag
      721 catgatgact acaccgcgaa tcccgccatc ttgaacgatc agattgcttg catttcctaa
      781 taccatccaa ggtatatcat ggattcggca ataatcgatg atttgcttta cttcttcttg
      841 ttttttcggg aatgcaagga catcagctgg tccacctgtt ttcgtaaacg tatagttcat
      901 caatggttca tcaaacaata tagtaatcgt ggctaagctt tcaactattt cttctttatt
      961 cactgaggat tttcctttct tttctcaaac gtcctcatta ttctatcacg cttaatacaa
     1021 tcttgccagt cttgttcaaa gttgatgacg gtagtgatct ttaagcactt cctcacataa
     1081 ttcatgactt cttacaactt ctttttgttt aagcaattct gtttccgatt tacctaaacg
     1141 gatctgagaa aagaaggcct ctaccattgg ctcaaacccg cgtttgacta gcgtgtttgt
     1201 ccagtcgcca aattctttca ctttttttcc atcagcatct tgaatgacaa gctgggtcag
     1261 atcgtttact tcataagtac catttttact tgttacttga tatttttcta tattagcgcc
     1321 acttgctaaa tccatcgtta aaatagccgt ttgattttgt gtttctaatt gtaaaatggc
     1381 atattccaaa aattcctttg tttctcttat ctggtatttt gttctcaaaa tgggttcatc
     1441 taataaatac acagctgtat caataagatg taaaaacaaa tcataaagga cgaactctgt
     1501 cgtttctttt gccgctattc gatttttttg caggtggatc aaccgcttat ctgggatacg
     1561 tttcaattct tctaccatag gagcaaagcg ccggttgaaa ccgatcatca aaattatttg
     1621 gttcttatca gctaattctt gcaattcacg aacttcattt agatggacac tcagtggttt
     1681 gtcaatataa acatggatac cattttctag acattttttg gctaattcat aatgtacttt
     1741 tgttgctgcg tggatcatac atgcttcgat tttttcttct aaaagctcat cgatcgtttc
     1801 atatagatgt tggaacccgt actctttttg aattctttct tttgtttctt tatttcttgt
     1861 tgcaaaataa aaatcaagtg ttccttgttt tttcgcataa gttggtaaat atgctttttc
     1921 tgcgatattc cctacaccga ttacaccaat cttcatctac tttcctcctg gatcatttag
     1981 ttactttcat cttacaccat tttttactaa aatgaggtac aatagtatcg caaaggtaag
     2041 gaaattaatt cgaaggagtg tgcttttatt gaaatttatc tcttggaacg taaacgggct
     2101 tcgtgccatc gtaaataaaa attttttaga agtttttcat gagttagacg ccgatttttt
     2161 ctgtctgcag gaaacgaaac tccaagcagg acaaattgat ttagatcttc caggttatta
     2221 tcaatattgg aattacgcgg aacgaaaagg ctattccggc acagcgattt ttgctaaaaa
     2281 accagcattg aatgccactt acggaatggg tatcgatatc catgatacag aaggccgtct
     2341 gattactttg gaatattccg atttctttct tgtaacctgt tacacgccaa attctcaaag
     2401 cgaattaaaa cgtttagatt accgattaga atgggaagaa gccttttata attatttgga
     2461 aaatctaaaa aaacaaaaac cagtcatcgt gtgtggggat ctaaatgtcg ctcatcaaaa
     2521 gattgatttg aaaaactgga aaacgaatca aaaaaatgct ggattcactc cagaagaaag
     2581 agcggctctt tctcgactgt tggataacgg gtttatcgat acattccgtt atttttatcc
     2641 aacccaggaa ggtgtctact cttggtggaa ctaccgattc aattcaagga aaaacaatgc
     2701 tggttggcgg atcgattact ttttgacgag caaagattta gagccaggac tagctgatgc
     2761 aaagatccat acggacatta tgggcagtga tcattgtcca gtagaattag atttgaaata
     2821 aagaggttgt gaaaaagtcg tttagtttcg agtattaaga aaaattttgt gaaaatggct
     2881 ttccacattt tgcataaatt tggcataata ccgagaaact ggcttttgaa cactgtttat
     2941 cgagagattg tgaaaaagct gtcctgcatc aagtaataag aacagccaaa caaaaatagt
     3001 tcctcatatt tttcgtttgg ttgggcttat taccgcagat acaagctttt gaacaccgtt
     3061 tattaagagg ttgtgaaaaa gctgcactgc atcaagtaat aagaacagcc aaacaaaaat
     3121 agttcctcat atttttcgtt tggttgggct tattaccgca gatgcaagct tttgaacacc
     3181 gtttattaag aggttgtgaa aaagctgcac tgcatcaagt aataagaaca gccaaacaaa
     3241 aatagttcct catatttttc gtttggttgg acttattacc gcagatgcaa gcttttgaac
     3301 accgtttatt gagaagttgt gaaatcagcg tcctgacctg agtattagag aaacaaatgg
     3361 caaaatagtt cctcatattt tgctattttt tgagctaata ccgagggtca gctgattgaa
     3421 caccgtttag tggagaagtt gtgaaaaagt cgcctacttt cgagtcgtaa gaaatttttt
     3481 tgaaaatggt tcctcacaac tttgcataaa tttgacttaa taccgagaag tcatgatccc
     3541 catctaactc caaatcacaa ggaatgagtt caaaaactag cttttctcaa caaatattac
     3601 ataaaaaatt ctatgttttt tgttaaattt atttgctttt ttcaaaagta tgttacagta
     3661 atttcccaag gaggttttac agaaatgaat tttgttgcca tggattttga aactgccaat
     3721 catcagcctt atagtgcttg ttctcttgcg ctagtcatgg taaaaaatag ccagatcgtt
     3781 gatgaattct atacattgat acagcctgag actccttttt tctggcgaaa tgtccaaatt
     3841 cacggaatcc atcaagaaga cgtacgcaat gcgccgaaat tcccagaagt ctggcaaact
     3901 atccaaccat attttcaaaa aaatcgttta gtcgttgcac ataatgctgc ttttgataca
     3961 aaggtcttag cgggatgctt agattattat catcttgaac agcctaatta tctttcttta
     4021 tgtacagtga aaagtagccg tcgcctgttt cctgaatttc caaatcatcg attgaacact
     4081 gtgtgcgaaa atttgaacat ccaattagat catcaccatg atgcattgga agatagtcgt
     4141 gcatgtgcgg aaatcctgct ttatcaagaa aaaaaattcg ggacagatcc attgaaaaaa
     4201 cttgtgacta taaagtaaaa gaatacttat gaaacacaca aagaggtcgt gagcctgccg
     4261 tttcactccg agtcacaagg agcaatttca aaaaatagct tctcatgttt attaaatatt
     4321 gtgactggtg accgaggagc tggcaggaga acaccgttta ttggagaggt tgtgaaaaag
     4381 ctgttctgca tcaagtaata agaacagcca aacaaaaata gcttctcata tttttcattt
     4441 ggttggactt attaccgcag atgcaagctt ttggacaccg tttacc
//
LOCUS       DAYYGO010000161         4416 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000161 DAYYGO010000000
VERSION     DAYYGO010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.166"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            59..913
                     /locus_tag="ACHA5T_002636"
     CDS             59..913
                     /locus_tag="ACHA5T_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFM8888162.1"
                     /translation="MENSIYLVTEPNTQLKDCYFNFCGYSKTEPRHSFGPAIREQYLI
                     HIVLEGEGYYSINDQKYFLKKGQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYY
                     LKEIGVGKNHLTFKVENLNSFKGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSAS
                     YLNEGIITKKMNPFVRQTIEIIRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISI
                     KEYISEIKISIANDLLSSTEYSIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRL
                     GLGIINGE"
     gene            1010..3259
                     /locus_tag="ACHA5T_002637"
     CDS             1010..3259
                     /locus_tag="ACHA5T_002637"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFM8888163.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3262..>4416
                     /locus_tag="ACHA5T_002638"
     CDS             3262..>4416
                     /locus_tag="ACHA5T_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8888164.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTR"
BASE COUNT         1556 a          625 c          806 g         1429 t
ORIGIN      
        1 gtaataatat aaacataata aaacaaaaat tatccaaatc taacatagga gggtggatat
       61 ggaaaattct atatatttag taacagaacc taacactcag ctgaaagatt gttactttaa
      121 tttttgtggg tattcaaaaa cagaacctag acatagtttt ggaccagcta ttcgagaaca
      181 atacttaata cacatagtgc tagaaggtga aggctattat tcaattaatg atcaaaaata
      241 ttttctgaag aaaggccaag gatttgttat cccaccagga gtatctactt tttatcaagc
      301 agatgaaaaa aatccgtggt catatgtatg gttgggttta agtggcaatc ttgtagaata
      361 ctatctaaaa gaaattggtg ttggaaagaa tcatcttacc tttaaagtcg aaaatttaaa
      421 ttcatttaaa ggattaattt ttgaaacttt atcgtatgat caggacaatc tagtcagtga
      481 attgtccaga caaaaacaag tttataaatt tttagaactc ctcttaaatt cagctagtta
      541 tctgaacgaa gggataatta ctaaaaagat gaatcctttt gttcgtcaaa caattgaaat
      601 aatacgtgag aatgcgtatc aaaaattatc tgttaaagat attgctgtca gtctatcgat
      661 acacgaaaat tatttatcac gattgtttaa gaatgatatg ggaatatcaa taaaagagta
      721 tattagtgaa atcaaaattt ctattgcaaa cgatctgtta tcatcaactg aatattcaat
      781 ccagcaaatc agtgacaaga taggattctc aacagtacaa tctttttcta aagcctttaa
      841 gaaagcaaat ggtgtttcac cgggagtata tcgaaaacgt agattaggtc taggaatcat
      901 taatggtgaa taaactaaac atttgatttt gataaatatt cgttacttta aacgcttact
      961 atttatgtga gcgtttttta taatgcaata aagaaacgga ggagagctta tgtataagga
     1021 taatgaaatg aatattattt atcgatcaga aaaccaacaa tttcatttgt ttaatcagga
     1081 gataagttac atttttaaga tatcggaaga tggtaaatta ttgcatttat attatggtga
     1141 gaacttacca gataatgact atagtcatct aattgagatg catcatcggc ctatgacaac
     1201 ctatagaaat gaaaacgatt tgctatattc gttagagcac ttaaaacaag aatttcctga
     1261 atatggtaca acagatttta gacatcctgc tatttcatta ctacagaaaa atggctctcg
     1321 cttatcagat tttgtctatc aagggtacga agtggaagaa ggaaaaccta aactagaagg
     1381 tttacctgct acctatgttg ataataatga tgaaagcaaa acattgaaag tttatatttt
     1441 agattcagtt acaaatgttc aagtagaatt attatataca atttatagag attatcctgt
     1501 tattactcgt agcgcgcgag ttatgaataa aggaaaagat attcaaataa tcgagaatat
     1561 tagcagttta tcattggatt tacccgatgc aaattatgaa tggcaacaat tatctggtgc
     1621 ttggggtaga gaaagaacta ttaagtcgcg tactttacaa caaggaattc aatcaatcga
     1681 atctactcga ggtatttcaa gccatcagca taacccattt gttactttaa aaagaaaaaa
     1741 tactgatgaa aatcaaggag aagctattgg tgcagcacta gtttactcag gaaacttctt
     1801 aattcaagca gaagtagata catgggatgt tactagacta caagttggca tcaatccatt
     1861 tggatttaat tggaaactca atccaggaga aatatttaca gcaccagaag cgattctggt
     1921 ttattcaagt aagggtttaa atgccatgag tcaaactttt catcaactat ttagaaaacg
     1981 cttagctcgt ggtaagtggc gagaaaaaga ccgtcctgtc ttaattaata attgggaagc
     2041 aacatatttt gattttgacg aagaaaagtt ggtaaatatt gctaaaaaag catttgatgt
     2101 tggtattgaa ttatttgtat tagatgatgg ctggtttgga gaaagagaaa acgattgtgc
     2161 aggattagga gattggcatg taaatcctaa aagactacct agaggaatcg gcagcctttc
     2221 agaaaaaatt cgtagttttg gtatgaaatt tggtttatgg tttgaacctg aaatggtgaa
     2281 taaggatagt gagttatatc gcacacatcc gaattggatt atttctacac ctaatcgaaa
     2341 acctaatcat ggtaggaacc aatatgtatt aaattttggg ttagatgagg ttgttgaaaa
     2401 tattttcgat caaatgtgta aaatcattga tgaatcaaac ttagattaca ttaagtggga
     2461 tatgaatcgt ccattgactg atgtatttga cagtcacttg tcagcagatc aacaaggaga
     2521 agtattccac agatatgtac taggcgtata tcgcttatat gaaaaactta taacaaaata
     2581 tcctaacata ctatttgagt cttgttcatc aggtggtggg cgctttgatc ctggaatgct
     2641 tcactatgca cctcaagctt gggctagtga tgattcagat gcaattgaaa ggttaaaaat
     2701 tcaatatgga actagtatgc tgtatcctct ttcgtctatt ggtgctcatg tatcaattgt
     2761 tccaaatcat caaacaaacc gtataacacc tataaaaact agaggaaatg tagctttttt
     2821 tggtgcattt ggatatgagc ttgacttaaa tgaattaagt gaaggcgatt tgaggattgt
     2881 aaaagaacaa attgcctttt ataaaaaata tagaaccata attcataatg gtacttttta
     2941 tagactatta tcaccattcg atgatgataa tcaaaccgcg tggatggttg ttagtcatga
     3001 taagaaaacg gctattgttg ctcattataa aacattaaat gaggtaaatg ctccttaccg
     3061 ccgactgaaa ttaaaagggt tagataatga cggactttat aatgtaggac aaaatagttt
     3121 gcgatctgga atggaattga tgagagcagg actagtctgt tcagatgcat ctagtggaca
     3181 aataaatgat cagaatcgac gcccagaaac atttgatttt gattccaaga tttgggttat
     3241 aacacaggag gaaaagtaga gatgaataac caaaaaagct tacttcaaaa aggagcaatc
     3301 ttaagtattt cactaatgtt aacaagctct caagcaatta atggtgttat tccacaaatg
     3361 aaacagtctt tggggatatc acaaagtcaa agtgaacttt taggaactgc accaagtata
     3421 actgttattt tgtttatctt attatctagt tactttgcca aaagaatagg aatgaaaaag
     3481 acaataattt taggtttatt attagctggt ataggaggaa ttattccagt atttacagct
     3541 accttccaaa ccgttcttat tagtcgagta atattaggtg ccggtttagg agtatataat
     3601 tcattggctg tgacatatat cagctcttta tactctggca atactagagc aacgctatta
     3661 ggtattagaa attcaatgga agctttaggt cagacaatcc ttatttttct tgctggagtt
     3721 ttagtgaaca ttaattggac ggcttcgttt ttagtctatg caattgcgtt tcctgtagct
     3781 ctcttattcg cgctaaaagt tcctgaaatt agagatgaaa atagtgacat acctgaaaat
     3841 catgtaaaag aaaagatgaa tccaatggtt tttgccctag ttctatttgc aatcttactc
     3901 gtaatgaata gtattgctat ttctgtacga tttgcatcaa ttgcaactga aattaaaggg
     3961 gtaaacttta atgctagtaa ctatttagct ctaatgccta tcttaggaat actagcagga
     4021 tttatgtttg gaccaataaa taagtggata ggaaaaggaa ccctatactt gggaattata
     4081 ttctttataa tttcaaattt gttaattgct gtgtcaaacg gtaatatgac gttcctgcta
     4141 acgggtttat ttttatcaag tataacagga tcatggtgct tcccatttat ctttagtaac
     4201 ttagataaag tcactacaaa gaatacaatt aattttgcta cttcattgat ttttattggc
     4261 tgtaatattg gtaattttat agctcctatt gctatgcaat tagcacaatt tctaacaaga
     4321 agtacaaatt tgactgctcc attttttgta ttcgcaggaa tatttattgt gatcctattt
     4381 gtaacatttt ttgcgacaaa taaaaaaaat actaga
//
LOCUS       DAYYGO010000162         4393 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000162 DAYYGO010000000
VERSION     DAYYGO010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.167"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..720)
                     /locus_tag="ACHA5T_002639"
     CDS             complement(<1..720)
                     /locus_tag="ACHA5T_002639"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8888165.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFK"
     gene            complement(730..2106)
                     /locus_tag="ACHA5T_002640"
     CDS             complement(730..2106)
                     /locus_tag="ACHA5T_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8888166.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENC
                     ELLKQKHK"
     gene            complement(2226..2873)
                     /locus_tag="ACHA5T_002641"
     CDS             complement(2226..2873)
                     /locus_tag="ACHA5T_002641"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HFM8888167.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(2957..4384)
                     /gene="uxaC"
                     /locus_tag="ACHA5T_002642"
     CDS             complement(2957..4384)
                     /gene="uxaC"
                     /locus_tag="ACHA5T_002642"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFM8888168.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
BASE COUNT         1444 a          883 c          559 g         1507 t
ORIGIN      
        1 tttaaataaa acatcagttc ttaagttttc ccatgattct ttttctttat tgaccaaaat
       61 aggataaaag aaaactttat ttttatttgc agcttctaga tcaccaggag aatcaccaat
      121 cattaatatc ttatccaaat cacaaccatt ttcaattaca cttcgaataa tatcttcttt
      181 ttttccttta tcttgacaac acagttcatc aactaatgat aataattcat gtctttccca
      241 ttcttcttga acagcttcct tattagctga gcttacaata attatcttcc catatttttt
      301 cagagctttt atagtatctc tagcacttaa aaagggttta tctaatcctt ttgcttgact
      361 aatctgttga ttgacttctt ccgaccattt taaagctttt aataaatcct ctgactgaga
      421 atattctatt tccctcttca aagatggatt agacaattct tttgttagag atacccagtg
      481 ctttaatcga ttaatcgatg ttacatcatt tttttctaat gttttaacta aacctaaaaa
      541 tctgtttatt ccacgagtct ctgaatataa attaatatta ttccaatcct gtaggaattt
      601 ttctctatga tgaacatcaa aaatctctgc agcaattggt ccgaaaaaga gattgtgttt
      661 ataagtcatc gtatccataa cacagccatc tgaatcaata caaaatatcc aattattcat
      721 atttctctcc tatttatgtt tttgttttaa aagctcgcaa ttttctaaga ctttatcttt
      781 agataatgga taaaggaaaa ataaaattaa tcctacaatc agaaaacaaa ttgatggaat
      841 taatgttgaa gcgttatata acttttctaa aacatctccc gtttgtacat ttgctaaaga
      901 gtcatagcct ataatagaaa gagtccaacc tccgacgcct ccagctagag cttgacctat
      961 ttttctagca aaagaatata ccgcatatac ggtcccatct tctctttgat cagactttac
     1021 ttcttgatca tcaataacat cagtaatatt tgcccaaata acagtattaa acacacccaa
     1081 acccatatag ccaaccgaac taagaattaa gaatacatac atattactag cttttatcaa
     1141 aaataagatc aaatatacta ttgctgaaaa gaacattcct actgatgcca gttctttttt
     1201 tccatatttt ttacttaaaa acaaagttaa aggcgaaaca actattaaaa ccaaaatagg
     1261 attgataaaa ttcattaaaa ctattccctg tgcatcatta tagtaatcag gaaatacata
     1321 attattcata ctcataatta ataattgagc aaggagtaag aaaatagccg caccaattat
     1381 agaaagtaat gccctatttt taactaaccc ttttaaagat tttaacaatg aaactttgta
     1441 ttgagattgg tcaattctta ctcgttcagt agttagttta taacatatta aataacaaat
     1501 aagtgcacat atcgaaaata ttcccgcaac aattgtaaaa gttgatccac cacgaactat
     1561 ttgagcgcct gaatcatctc ttgtataaat aaataatggt gttaatacac caatgaccaa
     1621 acttgctaaa gtagctccaa catttcggaa tgtcgatagc attgttcttt cttctgtaat
     1681 agaagtaata gctgatgcca ttgatccata aggaatattt atagatgtgt aaaaaatact
     1741 cccccataat atatacgtaa catacatgta tactacctta acgctcatag aagctgaaat
     1801 catagaagtt tgatacatta aaaaacttgc taaagcgact ggaccactca ttcttaaaat
     1861 ccacggttta aacttaccct gcttagttgc tttcgatcta tcaacaatac gccccatagt
     1921 tacatcagta actgcatcga taaatcttgc tactaaaaat aatgttccaa cggcagctcc
     1981 gcttatcccc aatacttttg tataaaagat cattaaaaaa gaactagcaa aaatgaaagt
     2041 aaaatcgttt ccaaaatctc caaacatata tcctattttg tctcttatac caaattttct
     2101 ttccatacaa ttctctccct tctgttgtta agcgttttca ttctgatgaa agtacgtact
     2161 atactctgaa gcgtaaagag tcagtattta ttttttacat ttcagagtat tatttgtttt
     2221 ttctcctaat aattctttat ctcataaaac ttatctatat atttactagc tactcgactt
     2281 acttcttcaa aatcaccttt ttcaacagaa gccaataaat tcccacctac accaactgca
     2341 acaactccta cttcaaacca atcttctaag ttctctatac ttactccgcc agtaggcatt
     2401 atatttacgt gtggtaatgg agcttgatag gcttttacaa tggatggacc aaaagcagaa
     2461 cctgggaata gtttaatgat atctacgcca gcagtaagtg cctcctttat ctcagtaatt
     2521 gtcatacaac ctggtaaata aggaatttga tataaattac aaatctcagc tgtttgtcga
     2581 tcaaaagatg gactaacgat atattctgcc ccagccataa ttgcgatacg agcagtaaac
     2641 gcatctaaaa ccgtaccggc tccgattatt acatcttttt gatcagcata ttttttagca
     2701 atttctttga ttatttcttc agcattagga actgtaaaag ttagttcaat ccctttaatt
     2761 ccacccgcaa taattgaatt tattgcttta atggcctcat ccttattttt cccacgtaca
     2821 accgcaatga ctccagccgc ttctaactta cttaaaattt ctactttttt catatctatc
     2881 tccttccttt atggatacat actttgtcta gctgtactaa cttacaaaga ataaatttaa
     2941 gcttttattt caagtattat tttctacttc cgatttcaaa aaaattaaaa taattatacg
     3001 cattacgata acaaatattc tccactatct gactaagata catttcatca tctggaattc
     3061 ggcctgcttt aacccactcg cttagtaatt gacataatac tcgacgaaaa tactcatggc
     3121 gaggatagct tagaaaactt cgagaatcag tcaacatacc tgtaaaattc gacaacaaac
     3181 tttgttgtgc catcgtagtc aactgttgac gcattccttc agctgtatca ttgaaccacc
     3241 aagcacatcc caattggatt ttttgaatca tacccccttg gaagctttgc attgctgttg
     3301 ctagtggaag ccaatcagtt ggatttaatg aatataaaat tgtttttggt aagcacccac
     3361 tcttttcagc ggtatcgaac aatgctacaa cattttgtgt caaactagct tgatccccca
     3421 tcgaatcacc accgacatct actccaattt gttcctttaa tatacttgaa ttatttctta
     3481 aagcatttaa atgcatttgc atcgtccaac catatcttgt atactcagac atcaaatgta
     3541 attgaatacc tgtaacaaat tgtgcaattt ctgataaatg gaaatccgtt tttcctgcta
     3601 aagcttttat taacagattg tcgagtattt tttcatctgc tatttcataa aaataagtat
     3661 tcaaaccgtg atctgctaat cgacagccaa tttgatgaaa atgatccact cgttggctca
     3721 atgcttgctt caacccatta aagtctttga ttggtatagc cgctgtttga ctaagtttta
     3781 aaatatagtt cgaaaagcct gatgatttga tactccagac cttatctgga cggaaagtag
     3841 gtaatacctt gaaagaatta tcctgtttag aaagctcagt atgataccac aagtcatctg
     3901 taggatcatc cgttgtacag atggctttca catccatcat ctccaacaat tctttcgggc
     3961 gataggaatc cgtttgaatt ttttcgttag cacgtcgcca aatttctggt gcattcttct
     4021 cagaaattgt caaatcaata tcaaagtaac gttgtaattc caaatgagac cattcaaaaa
     4081 taggattacc tggcgccttc tccactgttt ttacaaaagc taaaaatttt tcataatcat
     4141 cacctggacc actgatcaac tcttcttcta taccattagc tcgcatcaat cgccatttat
     4201 aatgatctcc agctccttta tcatttaacc atacctgagt caaattcttg tattgcttat
     4261 tttctagcaa atatgctggg tctaaatgac aatgatagtc gataattggt tggtgttttg
     4321 caaatttatg aaaaagtctt ttagctattt cattctctaa taaaaagttc ttatctaaaa
     4381 acatatttaa aca
//
LOCUS       DAYYGO010000163         4299 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000163 DAYYGO010000000
VERSION     DAYYGO010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.168"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..156
                     /locus_tag="ACHA5T_002643"
     CDS             <1..156
                     /locus_tag="ACHA5T_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFM8888169.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
     gene            175..1452
                     /locus_tag="ACHA5T_002644"
     CDS             175..1452
                     /locus_tag="ACHA5T_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8888170.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1462..2118
                     /locus_tag="ACHA5T_002645"
     CDS             1462..2118
                     /locus_tag="ACHA5T_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8888171.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2118..3494
                     /locus_tag="ACHA5T_002646"
     CDS             2118..3494
                     /locus_tag="ACHA5T_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8888172.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            3624..4037
                     /locus_tag="ACHA5T_002647"
     CDS             3624..4037
                     /locus_tag="ACHA5T_002647"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8888173.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIADCRQKLFQTRNSSLEQLFIYFVSNF"
     gene            complement(4062..4299)
                     /locus_tag="ACHA5T_002648"
                     /pseudo
     CDS             complement(4062..4299)
                     /locus_tag="ACHA5T_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611630.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1681 a          506 c          751 g         1361 t
ORIGIN      
        1 gataaggata agtgtattaa ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat
       61 tcagttgaga attgcaatca tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt
      121 ccagtggatg caattagtta tggagtaaga aaatagagag gagtagttag gaaagtgata
      181 aaaaatgcta tagcatatat tacaagaaag aaaaatagaa ctttaataat ttttatcata
      241 ttaactatag ttctttcttg cttgtattct tgtttaacaa taatgaaatc aagcaatgaa
      301 atagaaaagg ctttatatga aagttctaat tcttcaatat caataacaaa aaaagatggt
      361 aaatatttta atgttaatca atttaaagat attgaaaaat taaaagaaat tgaagaaaaa
      421 ataattcaat atgatggatt agcaaaacta aaagatgcta aagtagttag cggagaacaa
      481 agaataaata gagaagattt atctgacgaa tttaagaatg ttgtttcact cgaagctaca
      541 aataatacta aaagaaatat tttatttagt agtggagtat ttacaattaa agaaggtaaa
      601 aatatagaag aaaatgataa gaattcaatt attgttcatg aagaatttgc taaacaaaac
      661 aatctaaaat taggtgatga agttgatctt gaattactag atattgaaaa aagtggaaaa
      721 ataaaaagtc ataaatttaa aattataggg atcttttctg gtaaaaaaca ggaaacatat
      781 acaggattat cttctgattt tagtgaaaat atggtgtttg tagattattc aacaagccaa
      841 gagatattaa ataattcaga aaataatgaa attgcaaata aaattttaat gtattctggt
      901 agtgcagaat ctacagactt agccttaaac aaattgaaag agcttaaaat tgatgagtca
      961 aagtattttg ttgaaaaaga ttctaatgcg tttgaagagt ctttagagtc agtgagtgga
     1021 ataaaatata taataaaagt aatgacttat tctattatgt taggtgggat ggttgttctt
     1081 tcattaatct tgattctatg gttaagggaa agaatttatg aaataggtat attcttatct
     1141 attggaacat ctaagataca aattataatg caatttatat tcgagttaat attcatatcg
     1201 ataccaagta taatatcttc cttattttta ggaaatgtat tactaaaagt aattgtagat
     1261 ggattcatta actcagagga ttcaatgatt tccggtggaa gtttaataaa taatagtagt
     1321 tttatgtcaa atataacaac acttggacaa agttatttaa tattaataag tattattgtt
     1381 ttatcagttg tatttgcttc ttcattaatg ttaatcaaga agcctaaaga aatcttatca
     1441 aaaataagtt aggagaaaat aatggatata ttagaaataa agaatgtagc atatagttat
     1501 gcaaattcta aagaaaaagt tttgtcagga gtaaatcaaa aatttgaact tgggaagttt
     1561 tatgcgatag taggaaagtc aggaacagga aaatctacac ttctttcctt acttgctgga
     1621 cttgataaac ctcaaacagg aaaaatattg tttaagaatg aagatataca aaataaagga
     1681 tatagtaatc atagaaaaaa taatatatct ttagtatttc aaaactataa tttaatagat
     1741 tatttatcac caattgaaaa tattagatta gtaaataaat cagcagatga aagtatcttg
     1801 tttgaactag gtttagataa aaaacaaata aaaagaaatg ttatgaaatt atctggtgga
     1861 cagcaacaaa gagtagctat tgctagagca ttggtatcag atgctccaat aatactagct
     1921 gatgagccta ctggtaatct agacagtgtt actgctggag aaataattaa tatattaaag
     1981 acattagcta aagatagaaa taaatgtgta atagttgtaa cacatagtaa ggaagtagca
     2041 gattctgcgg atatcatttt agaactaagt ggtaaaaagt tgaaaaaagt aaataaaatg
     2101 aatttggagg ttgaataatg ataaaaaatg catttgctta tgtaactaga aaaagtttaa
     2161 aatcattaat tatcatatta gttattttat ctatgtcaac tttaagtctc attagtttat
     2221 ctattaaaga tgctacggac agagcttcaa aagaaacatt tgctaatata acaaatagtt
     2281 tttctatgga gataaataga caagtaaatc caggaacacc tagaggtggg ggaaatgtaa
     2341 aaggtgaaga tattaaaaag atatctcaaa cagatagtat agactcctat gtaaaaagaa
     2401 taaacagtgt tgcagattta gttgattatg atattattga aactcaagag actcttgcga
     2461 atcaatcacc tgaaagagca aagaatttta aaagaacagt tatgttaact ggggttaacg
     2521 actcaacaaa agaaacaaaa tttgtatcag aagcatataa attagtagaa ggaaagcatt
     2581 tagaaaatga agataaaaat aaaatcttaa tgcataaaga tttagctgaa aaaaataacc
     2641 ttaaagttgg agataagata aaaataaaat ctaatttatt tgatgctgat aatgagaaag
     2701 gtgcagatga aacagtagaa gtagaaatta aaggtctatt cgatgggcat aatagcggtg
     2761 gagtaagtgc agcacaagaa ctatacgaaa atacattaat cactgatatt catacagctg
     2821 ctaaagttta tggtaatacc gaagatacag ctgtatacca agatgcaaca ttctttgtaa
     2881 aaggtgataa gaatcttgat agtgtaataa aagatcttgg gaaattagat ataaattgga
     2941 gagaatataa tttgattaaa agttcatcaa attaccctgc attacaacaa tctatatcag
     3001 gtatttactc aatttcaaat aaattatttt ttggctcatt aatatttgca ggagttgtag
     3061 tttcattatt attattctta tggatgaatg ctagaaagaa agaaatagca gtattactat
     3121 cattaggtat atcaaaatta gaaatttttg gtcaattcct aattgagatg gtatttatat
     3181 caatccccgc attcgttggt tcctatttct tagctcaata tacagctgac aagcttggga
     3241 ataatatatt gaataaggtt actggtgata tagctaaaca aatagctaaa caatctgcat
     3301 ctagccaatt aggtggtggg gcagaagctg aaggatttaa caagacatta tcaagtttag
     3361 atatcaatgt attacctaaa ttcatgattt atgtagttat atttatgagt ttagtacttc
     3421 ttgtatcatt gattatttct tcattcaata tattaagaag gaatccaaaa gaattattaa
     3481 ttgacaataa ttaaaatttt cggtgctttt tagcaccaaa aatgagagtg taatataaac
     3541 cgtgtaagta accatcaatc ctaggcgggt ggttgcttca cggtttttat ttttctaaaa
     3601 taagatcaag aggtgattac acgatggcta gaaagaaaag aaatcctgat gctgagaagt
     3661 tagctgaatc cattttgaat acttatcaac ccgaatctgt agaggatatg caagatgttt
     3721 taaaagatgt gtttggtccc ttatttgaaa aaatgcttca aggagaattg aataaccatt
     3781 tagggtatga cgcccattct aaagagccaa agaaacatga caatcgacga aacggttatg
     3841 gcaataagac gcttaaaacg agctttggta aagtaaacat tgatgttccc agagaccggg
     3901 aggcttcttt tgaacctgag ttgattccta atagaaaacg agatgtctcc gacatcgcag
     3961 actgtagaca aaagctgttc caaactcgta actcgagctt ggaacagctt tttatctact
     4021 tcgtttcgaa tttttgaaca aaaaaataaa ggctccttgc ctttccatag ccaggtggct
     4081 aatttcttta aattcattgc agcaaacact agcatcgtat gcgtcgcgac tttttccaag
     4141 cctcgatact tggtccaacg catcccatgc ttttcttttg catccgcaaa tattcgttca
     4201 atcgtttgtt tccgtttttt gtaaattgat ttattcagat ctgttaggcg aagatgttcc
     4261 acttcatcca tcagatctcc ccaaacatgt ctcgtgatg
//
LOCUS       DAYYGO010000164         4284 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000164 DAYYGO010000000
VERSION     DAYYGO010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4284
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.169"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..268)
                     /locus_tag="ACHA5T_002649"
     CDS             complement(<1..268)
                     /locus_tag="ACHA5T_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888174.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSY"
     gene            complement(476..1402)
                     /gene="glsA"
                     /locus_tag="ACHA5T_002650"
     CDS             complement(476..1402)
                     /gene="glsA"
                     /locus_tag="ACHA5T_002650"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFM8888175.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(1425..2852)
                     /locus_tag="ACHA5T_002651"
     CDS             complement(1425..2852)
                     /locus_tag="ACHA5T_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8888176.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(2883..4097)
                     /locus_tag="ACHA5T_002652"
     CDS             complement(2883..4097)
                     /locus_tag="ACHA5T_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFM8888177.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         1342 a          913 c          766 g         1263 t
ORIGIN      
        1 aataagattg accgtacaga aactgtgtga gctcagaaag agcaatacca tgtttgaata
       61 cgagtttata cttatgcttc tctcttactt gttcttttat acaatattct agacactcgg
      121 ctagactttc tagatcgtga aggatcgttg gtgtagtcac ccttagagaa atagctgttt
      181 gttcgatgtc tatttcttgg aattcaaata attgttcaca cagatacact tttctaaata
      241 tatttcgctc gatgtaattt tccagcattc ctgatacacg cctccagttt ttcttatgat
      301 tttatttaat catatctgta aatcgtattt ttgtttcctt tttatttaga aaagcccttt
      361 caaaaaccaa taaaaaaaga aactgggaca aaagtcaaac aagctcacaa tttcagcctc
      421 attgtaggta gctttgagcc gtttggactt ttaccccagc ctctttcaag aatggttatt
      481 caaagatgtc agcgtctagt tctttgacta catctttcaa caattggata ccagctgcgc
      541 tgttaccaaa tggatctagt gccgggctga agacacctat gccgtaacga tttggcacag
      601 ctgccatcaa cccgccgcct acaccgcttt ttgcaggtac accaacatgg acggaaaact
      661 ctcctgattc atcatacaaa ccagcagttg tcataattga tttgacgatt gtagcacttt
      721 cttcagtaat gatttgtttg ccattccaag gagcaattcc tttatttgcc agcacagcag
      781 ctaattttgc tatgcctgta gcggtgacat tgagggagca ttgtctgaaa taagcatcga
      841 gtatctccgg tacgtctcct tcgatcattt gattaccttt catatagtaa gcaagagagc
      901 gattgatatc acctgttctt gattcagaat gatagatttc ttcattcaat gtgatattag
      961 gatcatcaca gatctctttc ataaatgaca agattcgctc aaatttttca tctgcatctt
     1021 taccggcaat caacgaggtt gttgcaatcg ctcctgcatt aacaaaagga ttcattggat
     1081 gtttgcgatg attgatttcc atattcatga tagaattgaa ggcaaaaccg gttggttccg
     1141 tgttgattgt ttgaaagact ttttctattc cgttgtcttt gattgctaat aacaatacgg
     1201 gaacttttga catgctttcg atggcaaagc gcacatcggc gtctcctccc tcaatcagtt
     1261 gttttgtatc caaatcataa atcgccatac ctaattgttt gggatccact tcagctaatg
     1321 ctggaatgta atccgccact tgtcctgttt gataatagtg gaaatttttt ccaatcaatt
     1381 gttctaattc ttttacttcc atttgaataa ccagctttca ttttttattt ttctgcatgc
     1441 tggaatttat gacggcatgc gtagatcgta aatggaataa ctaagacgat aagataagaa
     1501 acaatcagaa tcgtttcata aatcccatgc tgtccagctg cgatactagc aggtggaaca
     1561 aatgagatac aaaaagcaaa aagtgaagtc aaaagaccta aacaagcaac gattactttt
     1621 acaacttttc cgcctggaac ctgataggct ctttcaagac tgtcatcttt aaaaaccagc
     1681 ttcaaatagc ccgcgaaaaa cataaagtac aacatcaggt agattacaac agtcaatgac
     1741 atcgctgcaa gaaatgaaag attattcccc ccgccgctta atgtaaagac ggctgcccag
     1801 attgttgcaa cgatcccatt tgcgatcatc aaatgagcag gaacaccgtg tgtgttttct
     1861 tttgcaaaat attcaggaag taatccttct tctgctgcta cgtgaagtgc ttttgaagga
     1921 gaggttaccc aagaagcaat ttctccgatg acacctaaac aaacaagaat cgcaagaatt
     1981 ttcgcaatcc atgcaagatt tggatcaatg tgtcttaata ggaattctaa cgattgaatc
     2041 acccctgtat taagagacaa accatgttga ggtactgtag tagcgacggt caatccgcca
     2101 aaagtatcta aaatgattgc cagaataacc aatacaaaca ttgctaatgg ataatccttt
     2161 ttagggtttt ccatttcatt tgcatgactg gcagatgttt ctacgcccat atatgctaaa
     2221 ataaaggaaa caaaaacaac cagcgtaggc aatttcgaaa aatcaggaat caatgttttc
     2281 atagacagta tcgtttgtag tggagcgcct gaagcgacat aatggatccc taagccaaac
     2341 aagactaatg ctggcaacaa gataccagct acaaaaccga tccgagcaat caatgaagtg
     2401 tatttggttc cttttaactg tgagagtaaa atagcccaga aaataatcaa taggattgcc
     2461 aatttcagat atttattgtt ttgtatagca ccaatacccg ttgcatagga taatgcacca
     2521 gttaagaaat acagcatggt caagtagcca actgttatct caaaccattg gaaaaagatt
     2581 gcggcaaaac cgaatcgttt tcccaatgtg cgactgaccc aagtatagac accgcctttt
     2641 tcccatcctt tcaccgttgc catctctgct gcgcacaatg ccacagggat aaaccatagc
     2701 aaaccgccta ataacaagaa aaatactaag ctgaagcctg aggtcgcgaa tgtgggatat
     2761 tcatataatg aaacgaccat tgagatggtc atagccaaaa agccaaagaa cgttaatttt
     2821 tttgcaggaa ttttcgtttt gctttgttgc atgaatcttc ctcttttcta tttgaatttg
     2881 tttcaattga gtttgttgcc aaagaagaaa aagtccaatc cttcttcttg ttcttctttt
     2941 gtagtagtga tcgttgtaaa gtgcatcgcc gattttaaaa tcactgttgt cagcacatag
     3001 gcatacacgg cagtaatgat gacaccaatc acttgaatcc ctaagaagta ccagctgctt
     3061 ggattcgtta gtaaatggct gttagatgca aagatcccta tcagaatcgt acctgtgaat
     3121 ccgcccatgc catgaacacc ccacacatca agtgcatcat cccattgttt ctttttacga
     3181 aaatcaacag cagcatgaca gacgataccc gcaatgatcc caatcagcat ggcacttttt
     3241 gcttctacat aaccggcaca aggcgtaatg gctgccaatc cagcaacgga tcctgttagt
     3301 acgtcgacaa aatcaaagcc atttcctttt acttttgcaa tgatcagcca agtaatcatc
     3361 gcaaatgcaa gagcgatgaa tgttgaacca aatgcattga ctgcttgttc atctgctcga
     3421 agtgcaccac cggaattaaa accgaaccag ccaaaccaaa gcagtcctgt accgatagca
     3481 gctgccataa gactgctatg ctgacttctt tctcctaaag gaatctttct ttgaccaagt
     3541 gtgaggatac atgctaaaga gccgaatcca gctgtcgtgt ggatgactgc tccaccagcg
     3601 aaatctctaa atcccatctg atccaaaaat ccgccacccc atatccagtg gcaaacaggg
     3661 aaataaatca gcagattcca aaaaataaca agtataatat agccttttaa attcaatctc
     3721 cctgcaaaag ctcctgtcat caaaggaacg gtaatcacga taaacatcag ttggtaaaga
     3781 aagaacatta aaaatggaat cgttgcacca tgaaacatgt tggggaagct tgtaacatgt
     3841 ctcatcaaaa agaaatctgc cgggtttcca ataacaccgc cgatatcccg tccaaaggcc
     3901 aatccgaatc caccaaatac ccacataatc gttgttatcc caacggagag aaaagattga
     3961 gacatgatcg ttagtatatt attcttccgt gctaatccgc cgtaaaaaaa agctagacca
     4021 ggagtcatca ggcaaaccat tgctgtacat aaaatcatga atgctgtatc tcctgtattg
     4081 ataggtaatg tattcatttt tttcgcctcc tttctacgat tacgagtata aaagaaagtg
     4141 tagttgtttt tttcttctgt ttttttgaat taatacttgc atttataaaa gacagacaat
     4201 taataaaagc ttgatgtgtt gtgctctttt tcgtttttca cacaaataaa gacaaaaaat
     4261 aaacggtctt ctttttatag aaaa
//
LOCUS       DAYYGO010000165         4244 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000165 DAYYGO010000000
VERSION     DAYYGO010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.170"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            75..503
                     /locus_tag="ACHA5T_002653"
     CDS             75..503
                     /locus_tag="ACHA5T_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFM8888178.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
     gene            490..732
                     /locus_tag="ACHA5T_002654"
     CDS             490..732
                     /locus_tag="ACHA5T_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8888179.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(1032..1247)
                     /locus_tag="ACHA5T_002655"
     CDS             complement(1032..1247)
                     /locus_tag="ACHA5T_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888180.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            1354..1668
                     /locus_tag="ACHA5T_002656"
     CDS             1354..1668
                     /locus_tag="ACHA5T_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8888181.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            1772..2950
                     /locus_tag="ACHA5T_002657"
     CDS             1772..2950
                     /locus_tag="ACHA5T_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8888182.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(3131..3316)
                     /locus_tag="ACHA5T_002658"
     CDS             complement(3131..3316)
                     /locus_tag="ACHA5T_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888183.1"
                     /translation="MLEYSGFFIKAVTLKKDIIIVLFFLLLLIGRFILIHYSPLPIIK
                     EKTGSGCSNYMLRKKNP"
     gene            3353..3646
                     /locus_tag="ACHA5T_002659"
     CDS             3353..3646
                     /locus_tag="ACHA5T_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFM8888184.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(3734..3880)
                     /locus_tag="ACHA5T_002660"
                     /pseudo
     CDS             complement(3734..3880)
                     /locus_tag="ACHA5T_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
BASE COUNT         1450 a          638 c          820 g         1336 t
ORIGIN      
        1 tcctagattg tagtaataat ggaaagggat agaagtaaaa cgagttgaat gaattttttg
       61 agggggagaa tattatgggc gactcagagg aaacccacat tcaacatacg tttgacagtt
      121 tttgtaagaa agtagtctgc aatgaggcgt taaatattca aaagaagtac gctagatttc
      181 gccaacggca aatatcaatg gaaatattgg aacaaaaagg gttaggtaca gagttctatt
      241 atttggaacc aaatttgaat agtagtggta cttttattgt ccttggtatg aaaatcttta
      301 tttccaatga ggagttggca aatgcgattg cagcattacc tagtcaaaga caagaaatca
      361 tttttatgtc cttcttcatc ggtcttaatg atcgtgaaat tggcgatatt attgggaaaa
      421 gtctcggcag tgtttggtac caacgacaaa tggctttgga agagctagga aagtatttgg
      481 gtgtcaaata tgaagcaaaa taaacgatat ataccattca taccactaac gacaatcaaa
      541 gccgcagctt ctggtgattc aattgccatg tccatcgttc tgtcaaaata tcaaaattat
      601 atttcacggt tagccatgaa accagtttat gaagaagaaa aaggaatggt tatgtatgta
      661 gatgaatata tgcgtagaca actggaaaca aaattgattg agaagatact tgaatttgat
      721 acaactcgtt aaagtatttt ttccctttcc ttttcattct atggaagttt tttatcgggt
      781 caaaataaag gatttccata ggctgaaaat agcctagcgc tctttgacaa atgaatagca
      841 aatctatcag tacatgtaga ttttgattct ctagttacgc catgacaagg ttagctttta
      901 agcgacaaat tttaatatga gaatcgcgac tacgataatg atacttccgt aaccaaatcg
      961 gcccgccatt actgggggga ggtgagattc ctatgtgctt tggcaaaaca gctgatagat
     1021 cagaaaaact actatttcca tatttcgatt ttggttggca tatttttcat gccaaattct
     1081 gtgattttaa tagcatcggc aagatttaat ttttcttcag ctctctctcc acttttaagt
     1141 ttttttactg tactcaagtt gattcctgtt ccgaatgcta ttgcattagc cgtagatttt
     1201 tcaagaattt ctttaattat ttttatgtct gcaattcctt gtttcatatt aaaaattcct
     1261 cttttctagt ccgttttgat ggagtactgg agtgtttttt tacaatttta aggggtaccg
     1321 tggtggtcgt tttgattttg agagagaagg gtgatggagt tggaagttaa aacaaatgtt
     1381 aattctctgg aaatagagtt ctattcaatt ttacaaaaaa aactggaaga ttacacggaa
     1441 aaattgcttg cagtcagtca tttacctctg gtattaacaa atactgattt aaagcgacta
     1501 tttcagataa gtgatagtac actgaaccgt ttagtaaaac ttggtgagtt tcctccctgt
     1561 tggtacggga ttcgaggaca ctattttcgt gatgatgttt tagaatggat gagaaagagt
     1621 gattcgaatg actatcgaga aaaattgcga ctacttcgat ctttataagt tacattttct
     1681 ttctaagatg cggtacaatg atgacataga tagaaagatt tccaaagggt ttctgcttgt
     1741 tgggttcctt caaaatagga ggaatcaata aatggaaaaa acagatcgat tcaagaagca
     1801 aatcagttct actggtagga tcacatggta ctttcaagca tatcgaacaa caagacgtgg
     1861 ctttaattca aaacgtgagg cgcaactcgc ttatctggaa atggagaaac aacaaaggct
     1921 taagaaaaaa attgttgcaa gcaatgacaa gtttagtgtt gtaggtgagc aatggttcca
     1981 atattaccgt tcgttaaatg aacaaaagga aataacctat gataagcgag aggaacaatt
     2041 aaaaattatt gttcgatgga ttggtgataa aaagttgtct gatttgacac cagagtgcct
     2101 tcaagaattg ctttttacct taaaggaatg tggcgttaat ggaacaaatg ttggctatgc
     2161 caaaaatagt ttacaatcac ttattcaagt tctaaatatg atttttaaat attgtcaaag
     2221 aaagaatttg ataagtgaaa atccaatgaa gtctgtgaga atgccaaaat atcagttaac
     2281 ggtaactgat ttaaaagaag ccataaataa tattgaggac aagtttctta ctgtcgatga
     2341 attgagaact tttttgaatt atggcattgt tcatgaggaa ttaccgatgt ctgtactttt
     2401 ccatgttcta ttttacactg gctgccgagt cagcgaggcg cttgctctac agcctggaga
     2461 tatagatttc aaaagaaatg aaattctgtt ctataagcaa acggcagtta aaggtaagag
     2521 taaggatttt cgaattgaaa ctactaagac agttagttct gctagacgag ttctagtaac
     2581 tcctctagtt atggaaaagc tacaacagtt gattaaggct ttgggtgaga tgaggcaaca
     2641 ttacaggttt attatagaag aaacatattt gtttgtgtat cttgattcta gtaaacgtgg
     2701 tgttccttat cgtcgagagt atgtaaatga tcacgtcaaa cggtgcgtga atcgctcagg
     2761 aattaataag ccatttcata ctcatttagc tcgtcataca atggcaagct tagtagcgga
     2821 acactgtagc tgggaagtct tgaaagatcg cttaggccat actgaccaaa caacttcgaa
     2881 aatttatcgc cacttaacgt caaatgaaaa ggtaaaacct ctgattgctt ttagttcttt
     2941 agagagttaa aaaaacggtg aaagcactgg tattacagga ttcttgaaaa acgatttttt
     3001 aacaaattag tcaatatgta gtcagaggta ataaaaatcc ttttatatca atactttaac
     3061 gacttttcac aagtatcaaa gaaatccgtc gttaatcatt gttatcagac tgagaaagaa
     3121 cccttggtat tcaagggttc ttttttctta gcatgtaatt tgaacaccct gatccagttt
     3181 tttctttaat tattggtagg ggagaataat ggattagaat aaatctacct ataagtagta
     3241 gtaaaaaaaa gagcactatg ataatatctt tcttaagagt aaccgcttta ataaaaaatc
     3301 cagaatattc taacataaat ggagtattct tagaatcaaa aggagaaaga agatggttta
     3361 tacttatacg gagcaagaaa agatgaaagt tttagacaca ttcatgaatg atggaaaagt
     3421 aaaacaagta ccaagttcaa caaaaaagaa atacatccta ttgaaagaag tcttgaaacg
     3481 ctttgaccat ggagtgactt atacagaaac agaggttaat tcgatactgc tgaacgtatt
     3541 ttctagtggc gattatgtcg aacaaagacg ttatctaatc acatttggct ttttcaaaag
     3601 aagttcagac ggtcgggcat atcagatgat gggaattgaa aattagaagt tttaatttat
     3661 actcattgaa aaaagttcaa gtatgcgata ttggttctac accgcatact tgaacttttt
     3721 ttgtcatctg tatctataat acaaagccaa gaacgttatt caaaatatgg atagctatga
     3781 taaaagctaa tgagcgattc gcagttaaat aagcaatcgt cagtaaaatc gttccaggag
     3841 cgtacatgaa aaaatcaatt ggatgcgtta catgaataaa gataattctc ttttatttgt
     3901 acgaataaat accaaaacgt acctcctaaa attagatttt ggacagatgc cctttaaaaa
     3961 gataacaaga tttcgttctt gaaacaatat atatgctttt attccaatga ggttgtgaaa
     4021 acagtatttt gtcctgagcg ttagaaaatt gaaaatgggg aatagcaact cgtactttac
     4081 cattttttaa gtgaatacaa gatcagctaa ttgaacaccg tttattcgtg atgagggaca
     4141 caacactgca gtcgacacat ttctctttta tgttcaaaga taacaaatat ttaaaacaaa
     4201 gaaaaatagt agataatccc gtcctaactc ttttattctt ccct
//
LOCUS       DAYYGO010000166         4225 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000166 DAYYGO010000000
VERSION     DAYYGO010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.171"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            19..456
                     /locus_tag="ACHA5T_002661"
     CDS             19..456
                     /locus_tag="ACHA5T_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888185.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWVFYLVKK"
     gene            551..1435
                     /locus_tag="ACHA5T_002662"
     CDS             551..1435
                     /locus_tag="ACHA5T_002662"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8888186.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPEAINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSILLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1657..>4225
                     /locus_tag="ACHA5T_002663"
     CDS             1657..>4225
                     /locus_tag="ACHA5T_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFM8888187.1"
                     /translation="MNQYVKKLIASILFVFPLLMVTSVFADVEKTSESTQSENQSFDK
                     TTFSEESITSETTESQSEAPTSNTRQEENKTSETSEVAEQTSDSSEEVQVRVVSADGY
                     SEAATTEELTQLLADESVAKIRLTQPLTLDRELEIKRDIVIDFGGLAHNFGTHHIYIN
                     ETPQSIEFQNFKGTAAHPGGLIPNIDGNAIIIAYMSDFWGNRYHFTGEIKFTGTLDLY
                     DGSKLGLIYAPRATVTLDGVSGVLDVQPVKEGMNAAPGKAYFCRSYQLNVINGSQLYG
                     PYLGKFYGFLDSGDETGSGKTAPGLHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVT
                     FKVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSRVAVSNDGKITINTATTGGFRLQG
                     DSSQIHVSSGGQISVKMAGDGDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSA
                     VRFENGTQKLTVTNGGSLKVRNAGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFT
                     VTGEKSNIDIRADSGATVDTTGDIDLNFMAGEKTYLVMVGKTGEDKTGIISGDVLNVD
                     LESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKAGVTFDNFEKDP
                     DYFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRV
                     PTNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSPVEITALTQGQTDE
                     NEGTGYNPWGEGEQGGLFEVQVPNEERLTTGDQVQIVAAKKVRGGQEVTELDSAKTTV
                     DVIPPEPAKLSTTLINAASRSITGTGEAGALVSLKKNQAVLSETTIDDTGKFVLSIPE
                     "
BASE COUNT         1377 a          712 c          958 g         1178 t
ORIGIN      
        1 gcacgtagaa gtagataaat ggagtggttt tgcaaatttc ttttatttct agaagttatt
       61 cacgtatacg gatttgtttt tctcacaaat aaaaaggcag gaagtacaat ggatcatttg
      121 tatataaaat atattcatca attttccgtt ttggtattag gcacactaat tatagcgggg
      181 attgttgcat ttattattca aggtcgtgac atattaacag tacatcaaga gtttttcagt
      241 tatgcatttg gtattcctct gatcgctata aggtctgata acaaaaagtg gaagaaacgc
      301 tattacattc tcttgctatt tttgtgtttt tatctgccac ctaagatttt tactttatta
      361 ggtatacaat gcctatcatt ttcttttatt tcctatatca ttgttttaat tttcctcagt
      421 ttcatagtat gggtatttta tcttgtcaaa aaataaatga atgtttcatg aacagtactt
      481 catcacagcc taattttttt ctttatgcta tcgttatatt aaagaaatca atcagaaggg
      541 aaagaaagtg atgaaaaaag ttttattagt ggtaaacccc agttcaggcg gtgaacaagc
      601 aaaggaattt gagcaattag cgatagcaaa attggaatcg gtgtttgatg aagtggtcgt
      661 cttgcataca aaaaaagcag gggatgcaaa aaactttact cgcgaagcgg ctacggaagg
      721 gtatcatagt gtgtttgtca tgggcggaga tggaacagtc aacgaaggaa tcagtggaat
      781 cgctgaacaa gaacatcggc caaatttcgg atttttccca ttgggtacgg tgaatgactt
      841 ggctcgagca ttagggatac cgctagaacc tgaggaagcg atcaatcatt tttcgattga
      901 atcagtcaag ccattggata ttggaaaaat aaatgacgac tattttatga atgtcgtcgc
      961 aattggctct atccctgaag caatcaatga tgtagatgcg gagaagaaga cgaaatttgg
     1021 aaaactagct tatttcatgt caggaatcaa acagttagct tccacacaat cctactcgtt
     1081 tcacgtagaa gttgatggaa agaaggaaga aattgagagc agtatcttgc tgataggact
     1141 aactaactca gtaggcggtt ttgaaacatt acttccaaat gcaaaagtgg atgatggtaa
     1201 gctccatttg gtttatttaa aagattcttc tttgatcgat acgcttaaaa cattgccaga
     1261 cctattaaaa ggagtagatg aatctacgaa aaatctagcc tatcttactt gtgagcaatt
     1321 agttgtgtca ttagctgatg aggaagaatt gacgacgaat gttgatggag atgaagggga
     1381 taaattacct gtgacaatca aagtgttacc ctcacattta caggtttatt gttaaaatgg
     1441 aggttgtgac cgaagagttg tcaggaggac atcggttgtt cggaataagg gactgtgaca
     1501 agacttttgt cacagccttt ttttgtacaa aaaagaaatt ttcacctaac agaaaaattt
     1561 gccataaaaa tacatatttt aaatatgaaa gtacaattac aaataaatta aaacgttaaa
     1621 ataaaattgt caaaacgtca gagaggatgg tggagaatga atcaatatgt aaaaaagctg
     1681 attgcgtcga ttttgtttgt cttccctctc ttgatggtga catccgtttt tgccgatgta
     1741 gagaaaacaa gcgaatcaac gcaatcagag aatcaaagtt ttgacaagac tactttttca
     1801 gaagaatcaa ttacttctga aactacagaa tcccaaagtg aggcgcctac ttctaataca
     1861 aggcaagaag aaaataagac aagtgagacc tcagaagtag ctgaacaaac gtctgatagc
     1921 agtgaagagg tacaggttag agttgtttca gcggatgggt acagcgaagc tgccacaaca
     1981 gaagaactga cgcagcttct cgcagacgaa tcagtagcta agattcgttt aactcagcct
     2041 ttgactttag acagggaatt ggagatcaag cgagatatcg ttatcgattt tggcggactt
     2101 gctcataatt tcggcacaca tcacatctat atcaatgaga cacctcagtc gatcgagttt
     2161 cagaatttta aaggaacggc agctcatcca ggtgggttga taccaaatat cgatggaaat
     2221 gccattatca ttgcttatat gtccgatttt tggggaaaca gatatcattt tacgggagaa
     2281 ataaaattta ccgggacact tgacttatat gatggttcta aattaggcct gatttacgcc
     2341 cctcgtgcaa ctgtcacatt agatggtgtt tccggtgtat tggatgtcca accagtgaaa
     2401 gaagggatga atgccgctcc gggtaaagct tatttttgta gaagctatca gctaaatgta
     2461 atcaatggta gtcagcttta tggaccatat ctaggaaaat tctatggttt tctagattca
     2521 ggtgatgaga cgggcagtgg aaaaacagca ccaggactcc atatcttgag tggttcaaag
     2581 gtctctcttg actatgatcg ggcagatggc gtcagcgatg aaggagaggc tgtcgatacg
     2641 ctacccggga atgtcacatt taaagtcagt ggctcaggaa gtgaattttc ggtaaataca
     2701 aagatcaata ttaccaacga caacaatcgc ggaattatcc aaatgagagg ctcaggatca
     2761 agagtcgctg tgtcgaatga tgggaaaatc acaatcaata cagcgactac tggcggattt
     2821 cgcctgcaag gagattctag tcaaatccat gtatcaagtg gtggacaaat ctcggtcaaa
     2881 atggctggag atggcgacca acctggaaac aatgggatgc gttttgtagg gaaaggactt
     2941 tctttgatcg tagaaggagc cggatcagtc atcgatattg ataagcaaag tggtcgttct
     3001 agcgcagttc gttttgagaa cggtacgcaa aaactaacag taactaatgg aggctcattg
     3061 aaggtaagaa atgcagggga tggcaaatct tatgtgaaca gactcaatca aggaaaccaa
     3121 gcgatccaat tttatgacgc ttctggtttt ctagaaagtc cagggtcagc tgattttacg
     3181 gttactggag aaaaatcaaa tatcgatata cgcgcagatt ccggagctac cgtagataca
     3241 actggagata ttgatttgaa ctttatggct ggcgaaaaaa cttatttagt catggtcggg
     3301 aaaactgggg aagataagac tggcatcatc agtggggatg tgttgaatgt agacttagaa
     3361 tctcctacct atttcgattt ccaaaacaaa cggatcggaa gcgagactaa tggcataggt
     3421 ggctgggtat ttcaaggaaa tggcgattct tctttgaaga tcgctcattc agaaattgct
     3481 ttatggcgaa aggcaggagt aacgttcgat aactttgaaa aagatccgga ttatttttct
     3541 gatatgacag acttatcgat caatggaacc gacttaggta attttgtatc aagccctagt
     3601 gcaggtttaa caaatgaatt tgctcagaca ggtgccggaa tgaaaaatta taatcgaatc
     3661 agtgccaata accagcttcc gatgattgaa tccttaagag taccaacgaa tgcagataaa
     3721 aaaatctatg ggcatgtcgt tgttcccgaa ggtgtcgagg caaagccaag agatgcttgg
     3781 acaggcgaag tagaagtgac tttacgtatt acttatgcag atacgacgaa atcacctgta
     3841 gaaattactg ctctcacaca aggacaaaca gatgaaaatg aagggacagg atataatccg
     3901 tggggagaag gcgaacaagg cggtttgttt gaggttcaag tacccaacga agaaagacta
     3961 acaacggggg atcaagtgca aatagttgct gctaaaaaag taagaggcgg ccaagaagtg
     4021 acagaactgg actctgccaa aacgacagtc gatgtgattc ctcctgagcc tgctaaatta
     4081 agtaccacat tgatcaatgc agcttctcgc tcaatcacag gtacaggcga agcaggagct
     4141 ttggtttcat tgaaaaaaaa tcaagctgtt ttgtccgaaa caactattga tgatacaggt
     4201 aaatttgttc tttctatccc agaaa
//
LOCUS       DAYYGO010000167         4060 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000167 DAYYGO010000000
VERSION     DAYYGO010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.172"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(170..1675)
                     /locus_tag="ACHA5T_002664"
     CDS             complement(170..1675)
                     /locus_tag="ACHA5T_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8888188.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1672..2358)
                     /locus_tag="ACHA5T_002665"
     CDS             complement(1672..2358)
                     /locus_tag="ACHA5T_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8888189.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2551..3972)
                     /gene="gndA"
                     /locus_tag="ACHA5T_002666"
     CDS             complement(2551..3972)
                     /gene="gndA"
                     /locus_tag="ACHA5T_002666"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFM8888190.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1116 a          857 c          693 g         1394 t
ORIGIN      
        1 tttattgttt tttcgttcat ctaaaagata cttacattta attaacggat gttattctat
       61 ttaattgttc gctagttttt ttataatgac atccctgaat ttctcacttg cttgtatttt
      121 gtaccttatt ctttgttgga tatataaaaa aacaagtaaa gcacatgctt tacttgtctt
      181 ctgcctttgc gataggaata aagattctaa aaattgtccc ttgccctact acactttctg
      241 ctaagatacg tcctttatag ttttcaacca gttgtttagc gatagacaac cccagaccat
      301 ttccaccctt cgttctagct cttgctttat ccactcgata aaaacgattg aagatcttat
      361 taaggtcttc tttgggtatt ccttcgccaa agtcctgaat cgcgatctca aattcattca
      421 gagtggaaga aatagagata tggacttctt ttcgtgtagt tgaatacttt actgcgttat
      481 ccaatatgat aatgattaat tgttcaaagt gattacgata aatttgcagg gtcacttctt
      541 ttgtcaaatc atcatctaaa gtgatcacat agtctggata taatatcttg aaattattat
      601 aaacttgata ggtgacttct tttcctttag ttgtttcatt cgcataatga acatctactt
      661 gttctgctct tgataagtct agcatctctt gaactaagct tttcatacgg ctgatttctt
      721 gcatgctggc gcttaatgat tcttctaata tctcaggatc atctttcccc caacgattta
      781 gcagactaag atgcccttca ataattgcaa caggtgtcct taactcatga gatacgtctt
      841 ccacgaattg ctcttgttgt tcaatataca aacgcatacg gtcaagcatc tcattgaata
      901 tttcagctaa atctgccaat tcatcatttg tgtcgatttc aggcatgtgg atatctgatt
      961 gcggatcttt acgtatagta tccattgtat ctctgagtac cttgagtggt tttaagaaat
     1021 aagaagataa aaagaaacct aaaatactac ttaagatcaa agaaacaact tctaaaacaa
     1081 taagagtcaa taatagctta tttcgaatat catagaaaga agataattca tagaagcttt
     1141 gcgcatagcc gatcttttct cttgtacctt tagaatatat aggctggata gataaaaagc
     1201 cagtttttcc ttctagtgta acgatcgttg gaactttacg tgtagtctga atcaggtttc
     1261 gactgttttc atgggtttta aaaatcaagt tctcgtccaa atcataaaca taaagatcca
     1321 ttgatggctg ccctagctca gctataaagg aatcgatctt cattaagttt ccttcgacca
     1381 gtgaagtatg atctacgacc tcatctccgt catttgtcgc tttcgtcaga ttacgatacg
     1441 tatttactaa tgtcagttct tcatccgagt tagccaaacg agaagctact tctgaaatcg
     1501 tacgctctac attgtttctt tcttttgcca caatcagatt gatagatgat ttataggtaa
     1561 tgacagcgaa aattgtaaac acaacgaata tgaagaaaga actcgcaaag gcccacttga
     1621 ttgtcaagga gggccctttc agttctcttt tttccgattt tttcggcttt atcacgagcg
     1681 catcacataa cctgttccgc gtacagtttg gatatagctt tcctccccgg gaacgtcaat
     1741 tttgttgcga agataacgaa tatatacgtc cactacgttt gtttcaactt ctgtttcgta
     1801 tccccacact ttatttagta aaacgtcacg tgctaatacc acgttcacgt tttccattaa
     1861 agtaagcagc aactcgtatt ctctttttgt tagttcaatt acttcatttc cgcgacgtac
     1921 gacacggttt tctttttcga tagtcaaatc gcgataagta atagtagttt gtttagccac
     1981 atttttatcg ccttcaatat cgattcgacg caacaatgca cgaagacgtg ctaataattc
     2041 ttcgatagca aatggtttga cgatgtagtc atctgctccg tggtcaagtc cagatacacg
     2101 atcgatcaca gaatctcttg cagtcatcat aatgattggt gtatttttga cttggcgtac
     2161 acgtctgcag acttctaaac cgttcaattc tgggagcatc agatccaaca atatggcatc
     2221 ccactcatta tttaatgccg catctaaacc agttcggccg ttgtagtgga cttctgcgtt
     2281 atacccttca tgcttcaact ctagctcgac aaatcgtgcc aagttctttt catcttcaat
     2341 gattagaata ttgctcattt attattttat cctttctatt ctactggctt tttaaaaact
     2401 ctatcattat accgataata tgcttaaaaa tctatagtca ttttatcatg gatagcaatt
     2461 ttaaaaaaga gctgaagtct actcataaca atcgacttca gctctttcgt cctcttttta
     2521 ttttctcatt tcctaacagc cgaaagacta ttattcttca tggtaccatg agtaatggaa
     2581 gattccttct ttgtcgacac gctcataggt atgtgcgcca aagtaatcgc gttgggcttg
     2641 gattatattt gcaggcagac gttctgctct gtatgaatcg aagtaagcga tggctgatga
     2701 gaaagtcggt acaggtacgc cagcttggac agccaaggca acgacgtctc tgactgattg
     2761 ctgatatttt ttcgtgattt caacgaagta atcatctagc aataaatttt cgatctcagg
     2821 atttttgtca taggcatccg taattttttg caagaatcga gctcggatga tacatccagc
     2881 gcgccagatt tttgcgattt caccaaatgg caagtcccaa tcaaattctt ttgaagcgac
     2941 acgtaattga gcaaatcctt gggcataact catgatttta ctgaaataca acgcttctcg
     3001 gattttttcg accaattctt ttttgtctcc cgtgaactca aaatcgttcg tacgagacaa
     3061 aatcttgctt gcttgtacgc gttcttcttt ataagcagaa atatagcggg caaatacaga
     3121 ttcagtgatc aatggaagtg gcacacctaa gtcaagcgcg ctttggcttg tccatttacc
     3181 tgttccttta ttcccagctg catcaaggat cacatctact actggtttac ctgttccttc
     3241 atcgtcttta cgtgtcagaa tgtcagcagt gatttctatc aaatagctgt ctaattcgcc
     3301 ttgattccat tctttaaaga tatcagccat ttcttctact gaaaggttta aaatattctt
     3361 cattaagtca taagattcag caatcagttg catatctcca tactcaatac cgttgtgtac
     3421 cattttcaca taatgtccgg ctccatttgg gccaatatat gtgacacatg gtgctccgtc
     3481 ttctgctttt gcagaaattt gctctaagat tggtgcaact agcgcatagg cttctttttg
     3541 tccaccaggc atgattgaag gtccttttaa agctccttct tcaccaccgg aaacgcctgt
     3601 accgataaag ttgatgccag agttggctaa ctcttcatta cgacgcatcg tatctttaaa
     3661 gaatgtattt cctccgtcaa tcaaaatatc gcctttatca aggtgaggaa gtaactcttg
     3721 gattgttgca tctgttgccg gtcctgcttt gaccattagc aagatccgac gtggtttctc
     3781 gatagattct acaaattctt cgattgtata tgttgctttg aaatttttat ctgggtgttc
     3841 ttctactaca gcggttgttt tagcccctgt acgattgaat aaagccactg aatagccgcg
     3901 gctttctatg ttcaaggcta gatttttacc cataacggcc ataccgacaa cgccgatttg
     3961 ttgttttgac atatgaactc ctcctacata cgggatttct ccctaatctc ttacattata
     4021 gcttaacacg gctacattgc aaactaaaga acaacatatt
//
LOCUS       DAYYGO010000168         3951 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000168 DAYYGO010000000
VERSION     DAYYGO010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.173"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..1434
                     /locus_tag="ACHA5T_002667"
     CDS             85..1434
                     /locus_tag="ACHA5T_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8888191.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1562..2152)
                     /locus_tag="ACHA5T_002668"
     CDS             complement(1562..2152)
                     /locus_tag="ACHA5T_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFM8888192.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            2299..3651
                     /gene="brnQ"
                     /locus_tag="ACHA5T_002669"
     CDS             2299..3651
                     /gene="brnQ"
                     /locus_tag="ACHA5T_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFM8888193.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1128 a          729 c          775 g         1319 t
ORIGIN      
        1 aaattttgga atcttagttg tgtttttcag aaaactctct taatatggaa gtaattaaac
       61 ttttttagtt aggatgattt aaaaatgaat gaagagcaag aactgcagcg tggactgaaa
      121 aatagacatg tccaactgat ttctattggc ggagcgattg gaacggggtt gtttttaggt
      181 tccggaaagt ctatccagct agctggccct tcgatattgc tcgcctatct tattactgga
      241 tgcatctgtt ttttcattat gcgtgcgtta ggcgaacttc tgttatcaaa caccaataac
      301 cattcttttc tggattttgt tgcagaatat cttggcaaaa aagcggcttt catcactggc
      361 tggacgtatt ggttctgctg ggtatcgatt gcaatggccg atttaacagc catcggaatg
      421 tatgtacgct actgggcgcc tggtgttcct cagtggcttc ctgaactgat tgcattaggt
      481 cttcttcttt gcttgaatat ggtggctgtc tcattgtttg gtgagttaga gttttggttt
      541 gcattgatca aagttgtagc gatcattgcc tttatcctcg taggagctta tatgatcctc
      601 acccactata cgacagatat tggcacagcc agtatcacga atctatggtc tcatggcgga
      661 ttctttccaa ctggtgcaaa aggatttatc ttagctttcc aaatggtaac ctttgctttt
      721 gctgggatag aattagttgg attagtcgct ggagaaacag aaaatcctga aaaagtgttg
      781 ccggaagcta tcaataatat tcctatcagg atcatcttat tttacttagg atctcttttt
      841 gtcattatgg caatctatcc atggaacagc ttgaatccag ataacagtcc atttgtagaa
      901 gtattttcgg aaattgggat cacgattgct gcttccttga tcaatttagt cgtattaagt
      961 gcagcagctt ctgcctgcaa cagtgcaata tatagtacag gacgtatgct tcgttcttta
     1021 gcgcaagaag gcagtgcccc taaaaaattc aaaaagttaa caacacatca tgtgccaggg
     1081 aatgctttga cattttctac tatcgtcatt ttcatttctg ttatcttgaa ttacgtgatg
     1141 cctagtgaag tattcacact agtaagcagt attgccacta cttgtttctt gtttatctgg
     1201 agtatgcttg tctacacgca catgaaatat cgtaaaagta tcctcggcaa aaaagctcat
     1261 tcatttaaaa tgccgttgta cccatttagt aattatctgg tatttcttta catggccttt
     1321 gtgtgtgtcg tcttgttcct aggaaaagat acgagaatcg ctttattact aacacctgtg
     1381 tggttcttgc ttctattact aatctatcat atgaaatatg aacgtaagaa gtaagaaagt
     1441 agttgtgaca gaagtggtca gcttcgagaa ataagaagga atttacgaaa attgcttctt
     1501 aaatttttgt gaaatttcgg cttatttctt ggagataaaa gcttctgtcc cagcctcttt
     1561 tctatttccc cggaaacaac cgctgataat aggcatctgc ttttttatag gaaaaaatcc
     1621 aaataccaga tacaaaaaaa cgtccgaata acccgatcaa cggtacaaaa aagacaccgg
     1681 caatcaagca taagttaatc atcatggaga taagacttcg acggtccttt gacaaaactc
     1741 tcacgacatg ggaatctggt ttatttgctt tcatgatttg cctcatcaag ataccaaaag
     1801 aaaaactcca catcgtgtat actaagacat aagccatctc aggccaaaaa gcttcagggt
     1861 tacgcccgac ccaactagta acagaaggac ataaagtcag ccaaaagagc aaaaagatat
     1921 tcgaccagac cactcggtaa ttaattcgtt ctgccatctg aaatatacga tgatggttca
     1981 cccagatcac cgcaacaaat ataaagctga tggtataagc taataatgta tttcggatga
     2041 cccataaaga atgaaagcta gatgtattag ggatcttgat ctctaataca agaactctca
     2101 aaacaatggc taccactcca tcagtaaatg cttctaatct cgactttgtc acgatgattc
     2161 ctcctcagat gattcagcgc ttttatttta tcacttacgt tattaaataa ccatcgtttt
     2221 atatagggaa ttttctgaaa attgttgcat atttttcgaa attttgttac ttttaaaaaa
     2281 accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct ttttataggc
     2341 tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt tcatttgggt
     2401 caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac cggtatcggt
     2461 cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt atatgaatta
     2521 gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta cttagttatc
     2581 ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg tttagcacca
     2641 tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt attttttgtg
     2701 ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg aaagttcctg
     2761 aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt gaatccacta
     2821 ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt tttcacaggg
     2881 tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg gatcatcatt
     2941 gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa agacacgatc
     3001 aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct cgcttattta
     3061 ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac gctggctcag
     3121 attgctgatc attatttagg tacttatggc agtatcttat tagcatttat cgttatatta
     3181 gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt cacagatttg
     3241 tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc ttgtttagcg
     3301 gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat gttcatctat
     3361 ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa gcagcgtacg
     3421 gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga tgggttgaac
     3481 gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata tgttgcggaa
     3541 agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt tatcggtttt
     3601 gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg ataaaagagg
     3661 ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat ggcttctcac
     3721 atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg tttattgaga
     3781 ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa atggcttctc
     3841 acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac cgtttattga
     3901 gcgtctataa actgacctga gtattagaga aacaaaacgg caaagtagct c
//
LOCUS       DAYYGO010000169         3895 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000169 DAYYGO010000000
VERSION     DAYYGO010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.175"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..355)
                     /locus_tag="ACHA5T_002670"
     CDS             complement(<1..355)
                     /locus_tag="ACHA5T_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888194.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRINESDYNKRDQVLL
                     VKLNEEISVEEAVRTF"
     gene            complement(615..1163)
                     /locus_tag="ACHA5T_002671"
     CDS             complement(615..1163)
                     /locus_tag="ACHA5T_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888195.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1164..2021)
                     /locus_tag="ACHA5T_002672"
     CDS             complement(1164..2021)
                     /locus_tag="ACHA5T_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFM8888196.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2307..2593)
                     /locus_tag="ACHA5T_002673"
                     /pseudo
     CDS             complement(2307..2593)
                     /locus_tag="ACHA5T_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2583..2912)
                     /locus_tag="ACHA5T_002674"
     CDS             complement(2583..2912)
                     /locus_tag="ACHA5T_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8888197.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2989..3261)
                     /locus_tag="ACHA5T_002675"
     CDS             complement(2989..3261)
                     /locus_tag="ACHA5T_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM8888198.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(3261..3524)
                     /locus_tag="ACHA5T_002676"
     CDS             complement(3261..3524)
                     /locus_tag="ACHA5T_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM8888199.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            3758..>3895
                     /locus_tag="ACHA5T_002677"
     CDS             3758..>3895
                     /locus_tag="ACHA5T_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFM8888200.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LK"
BASE COUNT         1166 a          784 c          498 g         1447 t
ORIGIN      
        1 taaatgttct tactgcctct tccacactaa tttcttcgtt caatttcact aataatactt
       61 gatctcgctt attataatcg ctttcattta tcctaagagt caaatctttt tccaccataa
      121 cttttacacc caataaattt tgcggtttat ttttctctgt tgaaattctt tgtgtagcct
      181 ttaaactgac aattagctca ttgggtacat actctatttg agagtcatct aattggctca
      241 taatcctttc tttttggtta gtatctgcag ttacttgttt agtatgagcc atgaacccaa
      301 acatactcac cccaacaacc aatcctaaat atattttctt tttaaacttt ttcatctaca
      361 tctcctccta atagttttat tttagagtta gctctattac ttatttacta actcaacaac
      421 agattatcat acacttgtta atgaaatcac aatatctcat taaaacacac cctaaaatca
      481 taaccgttcc tatgctcatt ttttggattt tttaatcacg taaccagaaa aataaatccc
      541 ctctatttca cgcaaatcaa agaagaacta agtaactacc gtttaaaggt agttacttag
      601 ttcttctttg attattatct aactttatta ttcattcccc accctttgcg cagtgagtct
      661 tcggtatctt gttttatctg actggtagtt cttattattt ctccacgatc atctaattct
      721 ggaacaattg gatatttttc tctgaacaag tctagttctt tcaccacgtc attaaacaaa
      781 tcttgcttat cttcttgcgt aagattctgt ttaatatact catctaaagc ttttctaagc
      841 aattcatctc ttgtcatatt agtctcatac ttttctacta aaactctgga agtcgcattt
      901 aacaaagtcg acagttttaa aggatgcttg gtggaataaa ccttatcaaa ctttctgatt
      961 ggtgcgccaa ctttttttgt tcttcctcta ctctgtcttt caacattgga ggaagttttt
     1021 atttcttgaa cttggtgcac ttctaattgc ttattttcag atggtgtaat tgcttgctgc
     1081 tttttctttg cttctgcttc tttttttatt ttggcgagtc ctttgttgtt cccaaaaata
     1141 tcaagtcctt tgatgtcagc caattactcc acctccgcat accaattctc gtgttcttcc
     1201 aattctttca aaatactgat aaataattcg tggcttttgc gatcccattg ttttaagaga
     1261 cctttctttg tcttctccat cgtaatacct gatccgtcgt atcgttttaa gcgcgcattt
     1321 ttgagtacaa catttttaat caaatgttcc ccataaatct cttgggcttg tttatacatt
     1381 tcctgatcta ctgcgtcacc gctttccaac atgaaaggaa taacaccagc aacatataaa
     1441 tttgtatcaa attctttcac aaaaaagtcc atatactcaa gatattgcat cgcatttctc
     1501 atcgatttta cttgggtctg taagacgacg attgcccaat ccgcaatgta catcgcggat
     1561 ttactatata cagaaataga aggtgggaca tctaaataga tgacgtcaaa ctcatcaaat
     1621 aatggttcta gcttttcttt catatatttg aattgggata atgaatcatt atattggtct
     1681 ttgatccaat catcatacat ttcaaaggac atgtctgctg gaagtatata gaggttattg
     1741 attactttat agacagcctc tttaggtgga agattgttta tcaacacgtc agtcacagta
     1801 ttttcgtaaa caatcggttc accttctccg cctccttctt cgttagtgaa gttcatatgc
     1861 gttaagttca tcacatcaga agtatttcct tgcatatcca agtcaattat caaggttctt
     1921 tttccttcat taactgctga ttcaaaccct aacatttgcg cgcacgtact tttacctacg
     1981 ccacctttaa aattaccgat tacatatttc ttcgctacca cgttcctacc tcttttcttt
     2041 tagcaatatt catttgatac tattataaca tatttatttt tagttactac caaaaaaaag
     2101 atatatatgt actaccttat aattaataag taaaaggtag tacataaata aggaaaagct
     2161 gaaatatatt gatttatagc tttcctcaaa gtaatgacgt tatttaattt atcgagaagc
     2221 agagaatact cgcgacttta gtcatgtaga cgaatcagat tttcgataaa aaagaagccg
     2281 tccttcttaa gaacgacaac aaatcactag attaattttt tcaactgttc ataaaagttt
     2341 tcatgcgttc cacataacaa tactggaata accttgtttt ctataatctc gtaagctact
     2401 ctatatgttg tgcccgcata tttgaaccca ctagaccaaa tacctgataa atccccagtt
     2461 ttttgttcac ccttatatgg atcaatcgca atttcatcat aaattaaatt caaaaatttt
     2521 tctttcaatg gcttctcttt tagcttttta aatacttaat gacatgtttt gaaggaacga
     2581 tttcataagc caatttctgt tgccaactcc tctttagtca ttactttaga attagtaagt
     2641 gtatcttcag caatatctct aaaggaagaa tgcattttgt tttttctatt cttaaattct
     2701 acaagaatct catctttatc gtagccttct gcaattatat cagacaaaat atcttcgcta
     2761 aaatcataaa attctttttg tggttcaaca aattcataaa gaataccatt ctctaccaac
     2821 tttgcttcaa cttcgactcc attaggaaca ttaaaatctt taggtactgt caacatgatt
     2881 gaattgcctt gttttcttgt ttttgtaatc atttaaatcc ctcatttcta acagtatgta
     2941 attacttagt aaatacatta taccacgaga taatgtattt acaagtaatt actgtgactc
     3001 gtaatgcgtc cttgctgaat aaatttcaac cacgtgttca tcttcatcta ccttataaac
     3061 aacccggtgt tgtctattca atcttctaga atacctacct tcatgtgtag gttttaactt
     3121 ctcaaatgat tgggtaggga gatagggatc ttcctttaaa gtctgaataa ccttctcaaa
     3181 ttgaggtttc aaattagact gtttgatttt ctttaaatca gctttagccg agtttttaat
     3241 catcacagaa tagttattca ttataagtta tcccaatcaa tttcatcaac attagtaaat
     3301 ccactatcat ctttttctcg cttacgaaca acacccaaag tgcctatttg ctcaagtaat
     3361 agcgtttctt tgattgcttt ccaatcatcc aaaccaataa gcaccgcatt attttcactg
     3421 cgctcactaa tgatttcaat ttccgtatga ttctcattta catctttcaa caacttataa
     3481 aaatttttcc gtgctgagct aggatttaag actgtttttg ccatatctac tccccctcta
     3541 atttatatct ccattctaac gtacttaatt aagtacgtaa attcgattgt tttgtttaaa
     3601 ttggagtacc aaaacagcat tgtaaagtgt tatccgtttc ctttataccc gtggtgctag
     3661 ttaatttttt cctagaggaa aatgtctaaa catgttggta aatcaacaaa aaaacgcata
     3721 gagtagtctc aaaactccca agaaatgagg actacatatg cgttcatcaa aatatactga
     3781 acattataca gatactttta taacattcaa ggaaattatg agaactgttt ctaacatata
     3841 ccacaattgt gtaccagata agattaaaaa ccgaagaaat actgatcaac tgaag
//
LOCUS       DAYYGO010000170         3850 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000170 DAYYGO010000000
VERSION     DAYYGO010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3850
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.176"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..1155)
                     /locus_tag="ACHA5T_002678"
     CDS             complement(1..1155)
                     /locus_tag="ACHA5T_002678"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFM8888201.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            1340..3781
                     /locus_tag="ACHA5T_002679"
     CDS             1340..3781
                     /locus_tag="ACHA5T_002679"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFM8888202.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT         1231 a          721 c          840 g         1058 t
ORIGIN      
        1 ttatattttg tagtaacgaa tatcattttc gatctttgta atcgaaaaag gtacatctga
       61 agtatattct gcacattctc cgtgttcagg aagcgtctca ctgaaaatcg tttcatattc
      121 gtcaacagtg atccgtgttc gagaatctag catctcttga tgagattgtg cgaagagata
      181 ttcttgatag ttttcttcca gataacctgt aaaaaattca cttactgcac cagaaccgta
      241 gctgaataga ccgatgcgat ctcctggctg tagtgatttc gaattttcca gtagagaagt
      301 tagccccagg tataatgaac cagtgtaaag attaccgatt cgtcggctgt aacggatgct
      361 ttcttcatag cgagccataa gacgttcctg attttcttcg tctgtttggt ctaatacgct
      421 ttgcaatgcc ttttttccca tcttagtata cggaatatgg aaagcaatcg cctgataatc
      481 ttcgagtcct cgacccgaca attctttatg tcgattccaa actttttgga atgattcgat
      541 atacgtagaa ttagataaag gaccatcaac aacaggaaat tcgctataat ctggacgcca
      601 gaaatcatag atatcttctg tcagaaatac gctgtcgtct tcaatcgata aaatacgcgg
      661 gttttgagtg atcatcatcg caacagcacc gacaccttgc gtcacttcac caccgcttgc
      721 caagctgtaa cgagcaatat cacttgctat gactagtact tttcgttctg gatgattttt
      781 gacatattct ttcgccatgt gcaaagctgc ggtgccaccg tagcaagcct cttttacttc
      841 aaaagaacga gcaaaagatt ggattttcag taaatggtgg ataattaccg cactcgcttt
      901 tgaatgatca atcccggatt ctgttccgac gatgaccatg tcgattagct cacggtcttt
      961 ttctgttacg attttgctgg cagcatttgc tcctagtgta atgatgtctt catttgcacg
     1021 attgactgcc atttggtctt gaccaatccc tatatggtat tttgcaggat catccccacg
     1081 gctttctgcc aactctgtca tatctaaata taaattagga ataaaaaagg aaagacgatc
     1141 aatccctatt ttcatttcaa cacctcttta cttatgataa aaaacttact ttactaaggt
     1201 atcataaaaa gattaaaaga aatataaaaa agagaatatt taaggattgt taaaatttaa
     1261 tcttagaaaa aaataactgg aacaagagct atttgttttt acagcaaaga atggtatatt
     1321 tttgtaggag gtggcttact tgaaagaagt cgttatgata gatgctgcaa gaacgccgat
     1381 aggaaagtat cgcggaagtt taagtccatt tactgcagtt gaattaggca cacttgtgac
     1441 taaaggatta ttagataaaa caaaactgaa aaaagataaa atcgatcaag taatatttgg
     1501 aaatgtcctg caagctggta atggtcaaaa tgttgccaga cagattgctt tgaacagcgg
     1561 tctccctgta gatgtcccag caatgacgat caatgaagtt tgcggatctg gtatgaaagc
     1621 agtgatctta gcacgccagc tgatccaatt aggtgaagca gaacttgtga tagccggcgg
     1681 aacagagagt atgtctcaag cacctatgct gaaacagtat cagtcagaaa caaatgaata
     1741 tggtgaacca atttccagta tggtcaacga cggattgact gacgcatttt caaatgcaca
     1801 tatgggatta accgcagaga atgttgcaac acaattttct gtgagcagag aagagcagga
     1861 tcgctatgcc ttgtcgtccc agttgaaagc agcacatgct gtcgaagccg gtgtattttc
     1921 tgaggagatc atcccagtca agatttctga tgaagacgtg ttatctgagg atgaagcagt
     1981 tcgtggaaat agtacattgg aaaaactggg cacgttacgt acagtattct cagaagaagg
     2041 aactgtaaca gcaggaaatg cttccccgtt gaatgacggt gcctctgtgg tgatccttgc
     2101 atccaaagaa tacgcagaaa ataataatct gccttattta gcaaccatca aagaagtagc
     2161 ggaggtcggt attgacccaa gtatcatggg aatcgctcca atcaaagcca ttcaaaaatt
     2221 gacagataga tcaggaatga atttatcaac aatcgatctt tttgagatca atgaagcatt
     2281 tgcagcttct tctatcgtag ttagccaaga actgcagtta gatgaagaaa aggtcaatat
     2341 atatggagga gcaatcgcat taggccatcc aattggtgca agcggtgcac gtatcttgac
     2401 tacattagct tacggattgc ttcgtgaaca gaagagatac ggtattgctt cattatgtat
     2461 tggtggaggt ttaggcttgg cagtcttgtt agaagcgaat atggagcaaa cacataagga
     2521 tgtgcaaaaa aaaaagtttt atcagcttac gccatctgaa cggcgcagtc aattaataga
     2581 aaaaaatgta ttgacacaag aaactgcgtt gatttttcag gaacaaacac tttctgaaga
     2641 gctctccgat cacatgatcg aaaaccaagt gagtgaagtg gaaatcccaa tggggatcgc
     2701 gcagaatttt caaatcaatg gcaagaaaaa atggattccg atggcaacag aggaaccatc
     2761 tgtcattgca gctgcaagta atggtgcaaa aatctgtgga aatatctgcg cagaaacacc
     2821 tcagcggctg atgcgagggc aaatcgttct atcaggaaaa tcggagtatc aagcagtgat
     2881 caatgcagtt aatcatcgaa aagaagagct gattctttgt gcaaatgaaa gttatccttc
     2941 aatcgtgaaa cgcgggggcg gtgttcaaga tatctctact cgggaattca tggggagttt
     3001 ccatgcatac ttatctattg attttttagt ggatgtaaaa gatgcaatgg gcgctaacat
     3061 gatcaactcg attcttgaga gtgtggcaaa caagttacgg gagtggttcc ctgaagaaga
     3121 gattctattc agtattttaa gtaattttgc tacagaatca ttggcttctg cctgttgtga
     3181 aatcccattc gaacgcttag ggagaaacaa agaaatcggt gaacaaattg ccaaaaaaat
     3241 ccaacaggca ggggaatatg cgaagcttga cccttaccgt gcagcaacgc acaacaaagg
     3301 gatcatgaat ggtatagaag ctgtagtggc agctacagga aatgatacac gtgcggttag
     3361 cgcttcgatc cacgcctacg cggcaagaaa tggattatac caaggattaa cagactggca
     3421 gatcaaaggg gataaactag ttggaaaact aacggtgcct ttggctgttg ccacagtagg
     3481 cggagcttcc aatatcctgc caaaagcaaa agcatcttta gctatgttag atattgattc
     3541 agccaaggaa ctagcacaag taattgctgc agtagggtta gctcaaaacc tggctgcttt
     3601 gcgtgcatta gttacagaag gaatccaaaa aggccacatg ggacttcaag ctcgttcatt
     3661 agcgatctca attggggcta ttggtgagga aatcgagcaa gttgcgaaaa aattacgtga
     3721 agcagaaaag atgaaccagc agacagctat acaaatattg gaaaaaatcc gggaaaaata
     3781 aaaaagcaat atctttagaa gaataaagca agatttacag ttattttatt tactgcagat
     3841 cttgcttttt
//
LOCUS       DAYYGO010000171         3843 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000171 DAYYGO010000000
VERSION     DAYYGO010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.177"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            368..415
                     /locus_tag="ACHA5T_002680"
                     /pseudo
     CDS             368..415
                     /locus_tag="ACHA5T_002680"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            431..2350
                     /gene="tet(M)"
                     /locus_tag="ACHA5T_002681"
     CDS             431..2350
                     /gene="tet(M)"
                     /locus_tag="ACHA5T_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFM8888203.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYTSGKLLEALELEQEESIRFHNCSLFPVYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIEIKEPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVA
                     QLPLGSGVQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2469..2636
                     /locus_tag="ACHA5T_002682"
     CDS             2469..2636
                     /locus_tag="ACHA5T_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFM8888204.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(2696..3049)
                     /locus_tag="ACHA5T_002683"
     CDS             complement(2696..3049)
                     /locus_tag="ACHA5T_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8888205.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            3554..>3843
                     /locus_tag="ACHA5T_002684"
     CDS             3554..>3843
                     /locus_tag="ACHA5T_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888206.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILL"
BASE COUNT         1295 a          592 c          751 g         1205 t
ORIGIN      
        1 caatttgatt tggtacttga aaagaacggg agtaattgga agattataga ataacaaata
       61 ttggtacatt attacagcta ttttgtaatc acgtactctc tttgataaaa aattggagat
      121 tcctttacaa atatgctctt acgtgctatt atttaagtat ctatttaaaa ggagttaata
      181 aatatgcggc aaggtattct taaataaact gtcaatttga tagtgggaac aaataattgg
      241 atgtcctttt ttaggagggc ttagtttttt gtacccagtt taagaatacc tttatcatgt
      301 gattctaaag tatccggaga atatctgtat gctttgtatg cctatggtta tgcataaaaa
      361 tcccagtgat aagagtattt atcactggga tttttatgcc cttttgggct tttgaatgga
      421 ggaaaatcac atgaaaatta ttaatattgg agttttagct catgttgatg cgggaaaaac
      481 taccttaaca gaaagcttat tatataacag tggagcgatt acagaattag gaagcgtgga
      541 caaaggtaca acgaggacgg ataatacgct tttagaacgt cagagaggaa ttacaattca
      601 gacaggaata acctcttttc agtgggaaaa tacgaaggtg aacatcatag acacgccagg
      661 acatatggat ttcttagcag aagtatatcg ttcattatca gttttagatg gggcaattct
      721 actgatttct gcaaaagatg gcgtacaagc acaaactcgt atattatttc atgcacttag
      781 gaaaatgggg attcccacaa tcttttttat caataagatt gaccaaaatg gaattgattt
      841 atcaacggtt tatcaggata ttaaagagaa actttctgcc gaaattgtaa tcaaacagaa
      901 ggtagaactg tatcctaata tgtgtgtgac gaactttacc gaatctgaac aatgggatac
      961 ggtaatagag ggaaacgatg accttttaga gaaatatacg tctgggaaat tattggaagc
     1021 attagaactc gaacaagagg aaagcataag atttcataat tgttccctgt tccctgttta
     1081 tcacggaagt gcaaaaaaca atatagggat tgataacctt atagaagtga ttacgaataa
     1141 attttattca tcaacacatc gaggtcagtc tgaactttgc ggaaaagttt tcaaaattga
     1201 gtattcggaa aaaagacagc gtcttgcata tatacgtctt tatagtggcg tactgcattt
     1261 gcgagattcg gttagaatat cggaaaagga aaaaataaaa attacagaaa tgtatacttc
     1321 aataaatggt gaattatgta aaatcgataa ggcttattcc ggggaaattg ttattttgca
     1381 aaatgagttt ttgaagttaa atagtgttct tggagataca aaactattgc cacagagaaa
     1441 aaagattgaa aatccgcacc ctctactaca aacaactgtt gaaccgagta aacctgaaca
     1501 gagagaaatg ttgcttgatg cccttttgga aatctcagat agtgatccgc ttctacgata
     1561 ttacgtggat tctacgacac atgaaattat actttctttc ttagggaaag tacaaatgga
     1621 agtgattagt gcactgttgc aagaaaagta tcatgtggag atagaaataa aagagcctac
     1681 agtcatttat atggaaagac cgttaaaaaa agcagagtat accattcaca tcgaagttcc
     1741 accgaatcct ttctgggctt ccattggtct atctgtagca cagcttccat tagggagcgg
     1801 agtacagtat gagagctcgg tttctcttgg atacttaaat caatcgtttc aaaatgcagt
     1861 tatggagggg atacgctatg gctgtgaaca aggattgtat ggttggaatg tgacggactg
     1921 taaaatctgt tttaagtatg gcttatacta tagccctgtt agtaccccag cagattttcg
     1981 gatgcttgct cctattgtat tggaacaagt cttaaaaaaa gctggaacag aattgttaga
     2041 gccatatctt agttttaaaa tttatgcgcc acaggaatat ctttcacgag catacaacga
     2101 tgctcctaaa tattgtgcga acatcgtaga cactcaattg aaaaataatg aggtcattct
     2161 tagtggagaa atccctgctc ggtgtattca agaatatcgt agtgatttaa ctttctttac
     2221 aaatggacgt agtgtttgtt taacagagtt aaaagggtac catgttacta ccggtgaacc
     2281 tgtttgccag ccccgtcgtc caaatagtcg gatagataaa gtacgatata tgttcaataa
     2341 aataacttag tgtattttat gttgttatat aaatatggtt tcttgttaaa taagatgaaa
     2401 tattttttaa taaagatttg aattaaagtg taaaggagga gatagttatt ataaactaca
     2461 agtggatatt gtgtcctgta tgtggaaata aaacacgatt aaagataagg gaagatactg
     2521 aattaaaaaa attccccctc tattgtccga aatgcagaca agaaaattta attgaaataa
     2581 agcagttcaa agtaactgtg attacagagc cagacgcaaa gacgcagagc cgataaaatg
     2641 agattaatac aatctcattt tatcggctct ttccgttatg tatggattct tttaattagt
     2701 cttcgatgtt tcttgcttcg ttgataccgc tggctaaaga ttccattaag gatagttctt
     2761 tgtctgtaaa gctatccatg tatttctcta tctgtaatcg tcgggtgctt tttaccaagt
     2821 tattagcagg taagaaaaat tcatcaacgg aaacatgaag taacgataca aggtcataaa
     2881 gaacttgtat gctggggtgt tgccctttat tttcaatatt agttaagtac cgtgggtcaa
     2941 tttcaatcaa tgctcccact tgttcacgag ttaaacctcg tttcaatcga gcttctttaa
     3001 tggctaaacc aaaggctcta aaatcatatt tatcttcttt tttacgcata gtagaccacc
     3061 tctatacatt ttattgttcc tactgaatta aaaacaggta tagaaaaacg tgttatatgg
     3121 tttataggtt tatatttaat aaaaagcact actaaacgcc aataaaaaaa accgttatat
     3181 ggtagtgcta tttacgctgt taaaatattg tatattactt ccaaatggcg gtttgttgga
     3241 ggtcaacgtc gccatgaagt acatcatata caataaattt ccttacattg ggttcttgtc
     3301 aaaaaaagtc gtctatctgc aatagataag tacgtccacc aatgtggttt tataaatcat
     3361 atagatagaa taacagaagc atgtaaacag agaaataaat ctgtttatat gcttttttgg
     3421 ctattcagaa cttttttaca aagtttattt atcagtaatg caacaaatcc ccctttcaca
     3481 ttgggactaa gagtgaaagg agataaacga gcaaggctca cttcctttcc tagacagaaa
     3541 gggggtgaga aacatgaaac catcttcttt tcagaccaca atagaaaatc agtttgacta
     3601 tatctgtaaa cgtgctatgg aagacgagcg aaagaattat atgctttatc tttcaaggat
     3661 tgcaaagcgt gaggtgtcct tttcggatgt tggcgattat cttgttagcc agtttgcgac
     3721 aacagataac tattcaactg actttcagat ttttacactc aatgggttat cagtaggcgt
     3781 tgaaaatgat ttgttgagtg aagcattacg tgagttgcca gacaagaaac gtgaaattct
     3841 act
//
LOCUS       DAYYGO010000172         3835 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000172 DAYYGO010000000
VERSION     DAYYGO010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.178"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..342
                     /locus_tag="ACHA5T_002685"
     CDS             <1..342
                     /locus_tag="ACHA5T_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888207.1"
                     /translation="IFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFLMSLKPP
                     KSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYIGYFPNK
                     NNNFYFYRNKL"
     gene            complement(468..1145)
                     /gene="nth"
                     /locus_tag="ACHA5T_002686"
     CDS             complement(468..1145)
                     /gene="nth"
                     /locus_tag="ACHA5T_002686"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFM8888208.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1152..1850)
                     /locus_tag="ACHA5T_002687"
     CDS             complement(1152..1850)
                     /locus_tag="ACHA5T_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFM8888209.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2118..3302
                     /locus_tag="ACHA5T_002688"
     CDS             2118..3302
                     /locus_tag="ACHA5T_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFM8888210.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3308..3751)
                     /locus_tag="ACHA5T_002689"
     CDS             complement(3308..3751)
                     /locus_tag="ACHA5T_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFM8888211.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
BASE COUNT          992 a          757 c          634 g         1452 t
ORIGIN      
        1 attttttctg tacttttaag cactactctt aaaggaaaat ttatggaaag aagctctcat
       61 gcaatgattc tcactttttt atggttgttg ttcaccagaa tctatatttt tctgatgagc
      121 ctgaaaccgc ccaaatccat tagtttatgt ttagttataa atggtatttt tttattatca
      181 cttatcattt ttattacacc tttagataga tacgtcactt atttatataa tcctttggaa
      241 tactggactt attttatagg tatattggaa ggcattattt tctacatcgg gtattttccc
      301 aataaaaata acaactttta tttttatcgc aataaattgt aataagttac catcctagct
      361 actgagtatg tacatttact ttttcatgca aaaaagataa acgaaacagc ttttctccat
      421 aataacaaga taattcatac atttatttgc aattaaagaa accacgtcta caggcgtggt
      481 ttctttttta gcgccttttc ttttagacgc atccgatttt tcccatcttg acatattgat
      541 agcaaaggac atacctcgca cttgggattt cttgccgtgc aatgatatct accaaaaaaa
      601 atcagtgtat gatgtgtctt tacccataat tcttgtggta ctttccgcat cagtgtttct
      661 tctacttcca tgaccgtagc atctagtttg cagatcctca ggcgttttga gacacgttcc
      721 acgtgggtat ctactgcgat tgctggtatc ccgaatgcat cccccaatac gacatttgct
      781 gtctttctgc ctacaccagg aagagacatc aattcttctc ttgacgttgg cacttgccca
      841 tcgaaacgtt caatcagctg ctgtgcacac gctttgatgt tttttgcttt atttcgatac
      901 aaaccaatcg tttttatttt aagaatgatt tcatcgattg aagcttctgc taacgcatcg
      961 ggtgtcggaa aagaggcaaa caaatctggc gttgctttat tcactgatac atctgtcgcc
     1021 tgggcactta agatcactgc aatcaataac tcaaatggat tattgtgctt gagttctcca
     1081 tgtgcttcgg gaaacattcc atacatcgtt tctagagctt ccattgtttt ttgtttgctt
     1141 aacatattcc gctactcctc caaccaattt ttcagtgaga ctttcggcaa ttcttcttgt
     1201 tgagctgact gttctacttc tctttgaaga agctgctgtt ttcttcgttt ttggtcttct
     1261 tccacttgtt gttttgtacg tagatttttt ctttcccacg atagcaaaat gcggtcaacg
     1321 taattaaaac tataagcctg atttaatacg gcttcccgca gtgctagctt caaaatttcc
     1381 ggctgataat ggtcttcttc tagccattgt ccgatccgct ggaactcgat cgaagaaagc
     1441 gggcgtccaa attcttgttc aaataattga tacactgact ggatttcttt ttcttgtgtt
     1501 ttttcttcgt attttttttc ttgtgttttt aaatactcac ctaatagatc atagatgggg
     1561 taaagattat atcgatctgc ttttttaccg ttgacaatcg tcgtttctat cttgataaat
     1621 ccatttgtta cgagacgatc cagtatttga aaaatctgat cttgtttgat tcccatatcc
     1681 atagcaatca tctgcaaatc agggaaagta tctccttcaa gctgtgccat atgcaactgt
     1741 agaacaaata aaaattcttc agattgtaga ccgatcctat gataattttt aagtaaaaga
     1801 ttagatatga ctgtttgtcc agcgcttaag taattttcta aattcaacat gatttctcct
     1861 ccgctcaatc acagtatacg taaaaaaaac atgggaagca agaacaggtt tcttttctat
     1921 ttacttcact gatcggtcag atttacaatg tcgtctactt gatttttgtt tgttcatgtt
     1981 tttgatgcgt atcttcactt ttcggaataa ataaattaag tagatttcgc tgttccttag
     2041 aatgttgaca aggaaacaaa attactatac actatttttg tttccaagtt aacattattg
     2101 cataaaggag tttttacatg tacagtttca caaataaaca ttctaaagtt tttggatacg
     2161 tctctgtctt attcactgga ttaggattta ctatcgtcag tcctgtactt ccgtttttaa
     2221 cgctgcctta ttctcactcc atacatcagc aagctttcta tatcactttg ctcatgtctg
     2281 tttatgctct agctgccttc ttatctgccc ccattctcgg tagcttaagt gatcattttg
     2341 ggcgtcatcc gatccttatt atcagtttat taggatcaag tatcggatat ttgctttttg
     2401 gtttaggaaa ttcgatatgg atgttgttcc ttggtcgtat cattgaagga ttgacggcag
     2461 gagaaatctc caccttatat gcttactttg ccgacatcac ggaacctaat gaaagaacca
     2521 aagtttttgg atggatgggt gctttagtcg gtataggaac aacgctgggt cctattatag
     2581 ggggtctatt agctgagttg gggaattctg ttcctatatt tattggagca ctctttactt
     2641 tcttaaatgc cgtttatggc tacacattta tgctagaaag ccttccaata aaaaaacgtt
     2701 cagtagattt aagtttctcc catgtaagac cttttcacca attacaacaa ttattcaaga
     2761 tttcatccgt tattccttta ttgacagcag gttttgctgt ttggttggca gctggctctc
     2821 ttcaatctat cttttctcaa ttttcgattg acgcctttca gtggaaggct gggttggttg
     2881 gtttaagctt ctctttgatt ggaatcttgg atactgtctc gcaattgttc attatgcctc
     2941 gattgctcaa aaaattttca gaacagcaga tcacaagaat agggatgttc agtgaaatcc
     3001 tcgcctatct attcatcaca ttatctggaa ttcttctact gccgattttg tttttatttg
     3061 ggattatctg ttatggtttc ggtgattcta tttttactcc tgtattcaat ggccaactat
     3121 ccaattctgt ctcagataac cagcaaggtc ttgtaatggg cggtacccaa agtatacaat
     3181 ctctatcgcg cgtcatcggc ccgctaatcg caggacagct ttatgctgca gcaccatttc
     3241 ttccgacggt attcggattt ttgcttatga tttttgccta ttatcactac aataaaaaat
     3301 agtagcttca tttgctactt cgtataaatt ctctgtatcg accgataaaa cgaaatactg
     3361 tgtcaaattc ttcttttgac atattttcaa atacagtagc atctcgctgt acccattttt
     3421 gatggagttg ctcgtgtcta ttccttattt tttgtccgat tggcgtcaat tgaaaataga
     3481 tttctttttt attctcttcg ctttgataag tgtttatgat ccctctagct atcagttttt
     3541 ttgttagctt actgatggct cctctagtca tgtaactagc attcgctaat tttgtcacgt
     3601 taggatcagg gattttcccg atatgttcaa tcacttctag ttcatttaga gtaaatcctg
     3661 caaattcttt ttccatttct ggtttattac gatgaataat ctcattagct aaatccagat
     3721 attcttctat tagcagttgt cttttatcca tttctacacc ttctttttct ttcattatat
     3781 tgtttttttg tttcctattc aacaaagata aaaatatttt gtaggaaaac ccaat
//
LOCUS       DAYYGO010000173         3812 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000173 DAYYGO010000000
VERSION     DAYYGO010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.179"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(192..485)
                     /gene="rpmA"
                     /locus_tag="ACHA5T_002690"
     CDS             complement(192..485)
                     /gene="rpmA"
                     /locus_tag="ACHA5T_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFM8888212.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(498..842)
                     /locus_tag="ACHA5T_002691"
     CDS             complement(498..842)
                     /locus_tag="ACHA5T_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFM8888213.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            complement(869..1177)
                     /gene="rplU"
                     /locus_tag="ACHA5T_002692"
     CDS             complement(869..1177)
                     /gene="rplU"
                     /locus_tag="ACHA5T_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFM8888214.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     misc_feature    complement(1197..1273)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            1459..2100
                     /locus_tag="ACHA5T_002693"
     CDS             1459..2100
                     /locus_tag="ACHA5T_002693"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFM8888215.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            complement(2162..3793)
                     /locus_tag="ACHA5T_002694"
     CDS             complement(2162..3793)
                     /locus_tag="ACHA5T_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFM8888216.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
BASE COUNT         1181 a          844 c          670 g         1117 t
ORIGIN      
        1 atagcaaaca atatagcttc catcaattga aaacgatagt tatcaaaaca tcctgattcc
       61 aaattggcaa aaaaatatta agtgtaaaaa gacaaagtaa acttcgtagc ctccactaca
      121 acttttattt ttataaacaa aaaaggctgt gacaaaagtc ttgtcacaac ctctttaatc
      181 ataagattca attaagcttc ttgagctact ggataaacag acacttgttt tttgttacgg
      241 cctttacgtt cgaaacgtac tacgccatca acttttgcaa ataaagtgtc gtctccaccg
      301 atacccacgt ttacacctgg atagattttt gtaccgcgtt gacggtaaag aattgatcca
      361 cctgtaactg tttgtccatc agcacgttta gcacctagac gttttgattc tgagtcacgg
      421 ccgttagatg tagaaccgcc accttttttg tgggcaaata attgtagatt catagataac
      481 aacatggtct gcacctccta tttatcatta attgttttaa tttggatata ttctgagttt
      541 tcagcttcta tcgattgtaa tcctaataaa agattttcta atagaatctc agcaatattg
      601 ttttgttctt gtgtcattcc tgaagtgact tcggtataaa gataaccgcc ttcttcttca
      661 ttcatttcaa caatcggctc aaaaccggct aatgaggcaa tcccgttgac agtgctgatc
      721 gctaacgcag aaacacccgc acaaacgata tcacttccat aaggaccagc atcagcatgc
      781 ccggtcaaag tgaatgaaac gatctgacct gcgtcatttc gcttaaatgt tcctttgatc
      841 atatataggc cttctttctc aaacgctatt aagcgttgat cgcgtcgata accacttttg
      901 tatatggttg acgatgacct tgtttacggt gtgaatgttt tttaggtttg tatttgaaag
      961 taacgacttt cttttgtttg ccgtgttttt caacagtacc ttcaacagtt gcacctgcaa
     1021 cagttggagc gcctactttt gtagattctc cgcctactaa gataacttcg tcaaaaacaa
     1081 ctttttctcc tgcttcaacg tcaagttttt caacatagat tgcttgtccc acttcaactt
     1141 ttacttgttt accaccagtt ttaataattg cgtacatgct ataaagcacc tccttctgat
     1201 atacttagac tcgccatccg aagtgacagc atatgctgtg cttattactt gttctgagcg
     1261 gttgtagctg tggagtccac aattacaaca gtactagctt accagaacag caaccgttag
     1321 tcaactaaat ttaaaatcaa aaagtaaaac aaaaattcaa gcaatacttc tgcttttcta
     1381 tcctcacagt tctaccaaat aaaatagtcg cattgtgttt cttttattct tataataaaa
     1441 gaaaaaggag gagcataaat ggaataccca aaagaactga tacaagaagc ttctttaaga
     1501 atgaacggcc gtcctgtaga aggatttatc gctgggcaag gacctcttca tcctaaactg
     1561 atgctcgtgg gagaagcccc agggaaaaca gagattgata cgcatgttcc ttttagcggt
     1621 caagctggaa aagaattgat gcaggcactt tcttcaactg gcttaacacg agaagaggtg
     1681 tatatcacca gtgctgtaag gagccgcccg tatcgcgtga ctcatcggat caataaacgg
     1741 acacaacagt cagaaactgt ttatccaaat cggacaccta ctcgttccga agtatttgcg
     1801 catgcaccaa tattggatta cgaactgatg aaggtccagc caaaattaat tgcgacactt
     1861 ggaaacattg gtcttcaacg tttacttggc aaagaatata cggttaccaa aaatcatggt
     1921 caactctata cagggcctgt cttacaatta acagaacaaa aggatgccta cgaatggtca
     1981 gaaaaaaact atcgaattct tccacttttt catccagcag ctattttcta caatcgctcc
     2041 cttgtacctg ttatccatca agactggcaa gtactagctt cacttttaca gcaatattaa
     2101 aaagaacaga caagaccata aaatcttcag tttacagtct tgtctgttct ttttcattgt
     2161 tttaatggct aattgcagta tatttcgcat gtaaagctgt caacgcatga gcaactgttt
     2221 gatgttgatg cgtgaatgta tgttcccaca agtgagaaag ttgtttttct acttctggct
     2281 gcaaggaagt atagataatc gtttgtcctc tttgctctgc tttttcttga aattcatcaa
     2341 aaactgcacg tgccgtcaaa tcaatacttg gtacgcctcg catggagaaa atgacaccat
     2401 cgtgatgatt tagttcttcc aatttatttt tgagtttttc tgaagacata aaaaacagtg
     2461 gaccactaat atacactact gtccagttat tcagcttttc cgtttctggc agccccattc
     2521 tttgccaatc gatcgtttct aatgaaatcg tgatagcagc gcttttgaca acaaaaaata
     2581 cacagccgcc gatcaccccg atcacgatag caatacttaa atcaaagata actgtacaaa
     2641 tcatcgtaag gataaacaat agaactgctg accaatattt attagtaaat agctccttga
     2701 tcgtttccca ttcattcata cgccaagccg tcacaatcaa aacacctgca agtgctggca
     2761 ttggaatctg tgacatcact ggcgcaaaaa caagcatgga gagcagtaag acaactgcat
     2821 gtatcatacc agctaatcta gtttgtgcac cagatttgat ggccacgctt gtacgggcga
     2881 tggccgcagt agcaggaatc ccgccaaaga aaggaagcaa cagatttcca attccttgcg
     2941 ccattaattc ttgattgcta tctaagggct tatttgccat tcggccagcc gaagcaccac
     3001 atagcaagct ttcaatcatt cctaataaag caatgctaat agctggaact gccacttgtt
     3061 gcaaggcaga aaagctaaaa tctcccatat tcaaacgatt actactgatc aatgtttgtg
     3121 gaatcttacc cacagttgct actggtaaat ggaacaatag cataactgct gttgccaaaa
     3181 tgatagccag cagcgaacta ggcattcgtt tcgcccattt ttttggatag ataaacatcc
     3241 ctaaaatgac tagccctccc atgatcaaag aggctgtgtt tatcggaaat cctatcgttt
     3301 gataactact tagtttttca ataacgtttg taccttccga gtgaacacca aataaattat
     3361 cgatctgacc taatgcaata atgatagcaa tacctgaagt aaaccccgta attacgggtg
     3421 ctgggataaa agaagtcagt gttcctaatc ggaaaagtcc tgctgcaagt aaaaatagac
     3481 ccgctaaaaa agtagcaagc aatacacctt gcattccctt tgttgccgca atagacatca
     3541 agatagctgc catcgcacct gtcggacctg atatttgata aaaaccgcct gataaaccgc
     3601 caataacgat accggcaatg atagccgtaa tcaaacctgc ggccgcatca gctccgcttg
     3661 ataccccaaa agccaaggct aaaggcaatg ctacagcagc tactgtgatc ccagccaaaa
     3721 gatctttctg aaatcttgaa agatcataac ctgaaaattc tttttttagt gaggaaacat
     3781 aatttttgaa cacctatcta actccttttt ca
//
LOCUS       DAYYGO010000174         3746 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000174 DAYYGO010000000
VERSION     DAYYGO010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.180"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1528
                     /locus_tag="ACHA5T_002695"
     CDS             <1..1528
                     /locus_tag="ACHA5T_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFM8888217.1"
                     /translation="LSFHLSRIKGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAV
                     MLYWMTKNLIVSYIIYVWLLNLCTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVD
                     IYYRSAIAEAIAITLVIPVLFYAYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMST
                     ALIIIFIFIRLVSKGFKNADFIAIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKP
                     SVTNLYAHASNVFDSLTEAMNNDLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGT
                     CLTWLATTSLVPWYLLQNTPAKLLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTREL
                     FYLFMTVLLLITLTVSSKKNFNQKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIA
                     SLENKEHIIKHEAKIGSQWVNLPYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEIN
                     HQPVKEQVNANGLVQISGVKGVADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR
                     "
     gene            1703..2536
                     /gene="msrA"
                     /locus_tag="ACHA5T_002696"
     CDS             1703..2536
                     /gene="msrA"
                     /locus_tag="ACHA5T_002696"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM8888218.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQGERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            2616..3605
                     /locus_tag="ACHA5T_002697"
     CDS             2616..3605
                     /locus_tag="ACHA5T_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM8888219.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
BASE COUNT         1309 a          597 c          693 g         1147 t
ORIGIN      
        1 tctttctttt cacttatctc gaatcaaggg gttatcgact atttttacca gtccgattaa
       61 ttatgagaca ttcaattata ctggatatgg tgtaaattat ttttatccct ttctgacttt
      121 ttttccagct gtaatgttat attggatgac aaaaaatttg attgtaagct atattattta
      181 cgtttggtta ttgaacttgt gtacaacact cgtagcttat cattatggcg agcggttttt
      241 aaaacaaaaa aaagcggcat ttttatttag ttgtctctat attttctctg cttatcggac
      301 agtagacatc tactatcgtt ccgcaattgc cgaagctatc gcaataacac ttgttatccc
      361 tgtattattt tatgcttatc aaatcatctc tggaaaagaa gaaaagtatc cgtctgtaaa
      421 gctagcattg agtatgagtt tgctagtgta cagccatgta ctatcaacat taatgagtac
      481 agcattaatt atcattttta tttttattcg tttggtttct aaaggtttca agaatgcaga
      541 ttttattgct atatttaaaa aattatttag tgcggcagga atgacattgg ttctgacgag
      601 tgctttttgg tatccgatgt ttgaacaaat gctctaccaa aaaatcaaca aacctagcgt
      661 gacaaattta tatgctcatg catctaatgt gttcgatagt ctgactgaag ctatgaacaa
      721 cgacttaact acttactcaa tgggactagt tggtctgtta tctttatgta ttcccctgat
      781 tttatttaaa aaattaacaa acatagaaaa aaagatttat tatggtacgt gtttgacttg
      841 gctagctaca actagtttgg tgccatggta tcttctgcaa aatacgccag ccaaattgtt
      901 acaatttcct tggcggatct taagcttgca aataatattc tcttctctga tattaacgat
      961 gatatttttc aaaaatagaa gatacaataa gacaagagaa ctattttatt tatttatgac
     1021 tgttttgtta ttaatcactt taactgtttc tagtaaaaaa aatttcaatc aaaaaataat
     1081 ttctcagccg ggacatattg ttgtaaacaa agaaacagta gaggcgtata ccactaaagg
     1141 aatggattat tttttatatg attattctcc tatcgcttca ttggagaata aggagcatat
     1201 aataaaacac gaagcaaaga ttggcagtca atgggtaaac cttccttata aaacaactgg
     1261 tagttctatc atatatagag taactaatga gaagagccaa gtaatcacac ttcctgtata
     1321 tgcttattta ggaacgaatg tagaaataaa tcatcaacct gtaaaggagc aggtgaatgc
     1381 taatggattg gtacaaataa gcggagtcaa aggagttgct gatattaaag taagcacaag
     1441 ttatacagta acgactatta tttcttatct tgttacatta actgcatatt tatgtttatt
     1501 ttttaaaata gttttccata aaaggtaaat taacttttca tttataaaat aaattgatta
     1561 aaaaggagaa tctgtcgata aggattctct cttttttata cgaaaaatct gtagggggat
     1621 gaagtaatcg cttatttcct cactttatga taaattacag ctaaagtcac tagcttgtct
     1681 tcttcggaaa ggaggagaat ctatggatga ggaacaaata ttcattcaaa cactttataa
     1741 tctgattttg aatccgaata cacgagattg ggaacgaaaa gtattgattc agacaaagaa
     1801 tgacataaga gaaaatatat ccgttaaaga acagttatca aaactagaag caacactacg
     1861 tccattagct atccgaatga atctgacgcc tgatgtaatg gacttttatt tattgattac
     1921 ggaaggattt gataaggagc aaaaatatga cttttccaag catgccatgc aagatgctga
     1981 ctatcaagaa cgtgcagtat ttgcaggggg atgtttctgg tgtatggtcg agccgtttga
     2041 atcgaaaaaa gggatcctat ctgttttgtc aggatacacg ggcggacatg ttgaaaaacc
     2101 aaattatgat caagtaagtg gtggttacac aggtcatgtg gaagctgtgg agatcattta
     2161 tgacacaaga gaaataagtt atagcgagtt gctgacaatc tattggcaaa ttactgatcc
     2221 cacagataca tttggacaat ttcaagatag agggaagcaa tatcgtccag tgatttttta
     2281 tcaaggcgaa cgacaaaaag aattagcgga acaatcaaaa cagaaactag acagttcagg
     2341 aactttccac caaccaatcg tcacaaaaat cgaaccagcc ggtacgtttt ggccagcaga
     2401 aaactatcat cagcaatttt acaaaaaaca accgaaacgt tataaaaaaa tccagcaagc
     2461 acgtaatcaa tttctgatct atcaacgagt aaaaaacaaa tggcaaaaaa atatacgaaa
     2521 aaatcatttt gattaactcc ttttctttaa aatgtagtaa actaaaaata gaaaaaagaa
     2581 agcgttttaa aaagatttga tgaggaggag aaaaattgta tattgcagat agtgcacgtt
     2641 atgaaaaaat ggaatatcgt aaatccggtc attcaggttt gaaattaccg attttatctc
     2701 tcggattatg gcaaaatttt ggtgattatg atcctatcca taatcaaaga gagattttaa
     2761 gaggcgcatt cgacatggga attacccatt tcgaccttgc gaataattat ggaggccctg
     2821 ctggagctgc agagaaaaac tttgggcgta tttttagaga agattttcaa gcctaccgtg
     2881 atgaactgat tatttctagc aaagcaggtt atcatatgtg ggaagggcca tatggggaat
     2941 ggggctctcg taaatccatc atttcaagtt gtgaccaaag tctccaaaga atggggctag
     3001 attatgttga catcttctat catcatcgcc cagaccctga tacacctttg gaagaaacag
     3061 cagaagcttt gatgcaactt gtaagacaag gaaaagcatt atatattggt atatccaact
     3121 ataatggaga agatacaaaa aaaatgactg aaattttaaa aagaaaggaa gcaccgttca
     3181 ttattcatca gatgcgctac aatatgtttt ctcgtgcact actagaagac gatctatctc
     3241 cagtattaga agaagaaggg ctaggggcaa tcacgttcag tccgcttgct caagggttgc
     3301 tgacaaatcg ttatctacat ggtattcctg aagattctag agcacatcgt aaggaaatac
     3361 cattcctttc agaagaacaa gttggatcaa ctctggaaaa aatcaaagca ctgcaaacaa
     3421 tcgccgtatc acgaggacaa tcattagcgc aaatggccct tgcatggaat ttgcgacaaa
     3481 aatctgttac aagtgtactg gtcggagcaa gtcggttaag tcagttacaa gaaagtgttc
     3541 gaatgatgga taatttagat ttctcaccag aagaagaatt gagaatcgac caaattctag
     3601 aatagaaacg atgtaatcaa actttttatc catatattaa aaaaaacaaa agatgacatc
     3661 aagaatcgaa tgaagatcgt ctgttattga acatcatatt ttttgtcgtt caaaataaaa
     3721 atgatcgaag gctaggagtg aatttt
//
LOCUS       DAYYGO010000175         3520 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000175 DAYYGO010000000
VERSION     DAYYGO010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.181"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2199)
                     /locus_tag="ACHA5T_002698"
     CDS             complement(<1..2199)
                     /locus_tag="ACHA5T_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen binding domain-containing protein"
                     /protein_id="HFM8888220.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESLSESFNEARSSEPSITQESQIER
                     QEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVRVPRASIGLIEF
                     IRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITFEVAFFQSGGTE
                     PNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGNLGNLLIDGQDV
                     TNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREPISGGVRIAEVT
                     NPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYALEVTDVDLFNE
                     GRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNVINLNENELTYT
                     LHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRFSANNTIGGPTS
                     FTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKVIKTDENGQRLA
                     GAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYLLDATVHDVFIN
                     EYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGETLQTG"
     gene            complement(2871..3139)
                     /locus_tag="ACHA5T_002699"
                     /pseudo
     CDS             complement(2871..3139)
                     /locus_tag="ACHA5T_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT         1002 a          777 c          546 g         1195 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tacattaaaa gactcacttg tgctttcttc tggtacactg atttctattg agaccgtatt
     1861 tgattgaata tcaccgcttc ttgcggtcaa ttcgtattta cctgaatgct gggaagaata
     1921 agtaatcaga cgttcgtaat cattgagttg cttcacttcc ccttcaatca aatcctcact
     1981 cttatcaatg accaatgcat ttagcaggtc tggtgttcct ctgactcgca actggaactg
     2041 ctcacccaaa gcagcctcct cttgatcaac ttcgatcgtc agcgaacgct ctggctgttc
     2101 tgctaaagac tcagctagaa ccgttatgtt cccaaattga ttgatcacaa taagcataac
     2161 tagaaacata cttaacagtt tatgaaattt gttcctcact attactcccc cttataattt
     2221 tgactaaaca acaaggtacc cgataatttt ctaaaaagat tccccaactc aaaatggctg
     2281 cgttacctcc ctcgtatttt cggttcaact caagcgttgc aatgtaagct gtaaacaaag
     2341 ctaatgattg ccgattgtct tctgttgatt gattcgatag ctttgtatga cttttagttg
     2401 tccgtgtggc ttaaccataa ttatgatttc acgattcctc ctgccagtgc cttgcacaag
     2461 ctggagcgta accctaatag ttattttgtt aatgtactta tatactttta tacttttcca
     2521 ttagaaacaa tcactaaatc gttgatacca aggtcagact ctagaatttc atcagtgagt
     2581 ccctgtattg gttaaccttc atcaatccca aaaaaatata tcaatgctta gcatcaaaat
     2641 gaatatatag tttagaatac ttgggattca tcgataactg atcgcttatt tggccatatt
     2701 atttggcact tggttcaact aaacgcattt aggatgttcc ttcattctcg tcatggatgt
     2761 tctcaaaccg tcattactaa taaactgtcc accgaatcga tggttcattt cttgcatttt
     2821 ccacgtccaa ttactctttt tgctctttat ttgggcgtag tttcactttc ttaacttgta
     2881 tcgtcttcca tcaactcctt aatcatcttc ttagctttat tggctcggtt tccttgtaaa
     2941 aggtatctac aaatagtgac aatctgtatt aaatcttcat cattttctgt aatgtcaata
     3001 atttctatat tctgtaccat atattgcaaa gggtttcact aagttcataa ccgaagcgaa
     3061 ttaaacaact tttgtataga ataactacct tttctatttt tccattggta attctatcta
     3121 ttaattggtt taatcctttt gttgtagttg attccactac caagatgatt ctctcgaatt
     3181 ttaaagcttg aagatgtatt gctatttttg ctaactggcg tatttgcaac tatttttccc
     3241 ttctattctt ccttcattga ttcccctcta aaattactat ccataaaaaa ttgcataaat
     3301 gacaatagta tttatattag tcatactgct ttttaacaca gcaaattcca gaaaaacatt
     3361 cgttacatat ttgtgaattt ttccaaaaaa taacattttt ataaaacgac gaaaaataaa
     3421 tcactaccag tcaacttcta taatattacc aaataacaaa ctaactatcc atcaataacg
     3481 tgttttattt tttaaaattt ctataaaaaa acaatcattc
//
LOCUS       DAYYGO010000176         3505 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000176 DAYYGO010000000
VERSION     DAYYGO010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3505
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.183"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            57..938
                     /locus_tag="ACHA5T_002700"
     CDS             57..938
                     /locus_tag="ACHA5T_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8888221.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            951..2561
                     /locus_tag="ACHA5T_002701"
     CDS             951..2561
                     /locus_tag="ACHA5T_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8888222.1"
                     /translation="MADKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2638..3297)
                     /locus_tag="ACHA5T_002702"
     CDS             complement(2638..3297)
                     /locus_tag="ACHA5T_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM8888223.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
BASE COUNT          960 a          679 c          699 g         1167 t
ORIGIN      
        1 attaaactat ttaatcttgt ttagtatata tttttagaaa aaggagtggg gtttttatgg
       61 cagaaatcgt caaagtcaca catttgaaaa agagctttgg tcgtttcgaa gctttaaaag
      121 atgtctcttt caccgtacat tcaggtgaag tcgttggatt tatcggacca aatggttctg
      181 gaaaatccac gacgattcgg acacttttag ggattataaa aaaagacggc ggaacggcaa
      241 cgatttttgg gcaagatgta tgggaagata gtttatctat ccatgaacgt ctttcttatg
      301 ttccaggcga tatctctttg tggggaaatt tgactggagg agcgatcatc gatctcttca
      361 tgaagcttca tggaaaaggg agtgctcaaa aaaaagatga actgattcaa cgtttctctc
      421 ttgatcctaa gaaaaaagca aaaaactatt caaaaggaaa tcgccaaaaa gtgggcttga
      481 ttgctgcttt ggctgttgat tcggatctct atttatttga tgagcctact tctggtttag
      541 atcctttgat ggaaacgatt ttccaagaag aagttgaaaa attgaaagaa cagggcaaat
      601 ccatcctact ttcttctcat attctcagtg aagtcgagcg actagcggat aaagtcgtga
      661 tcatacgcga aggaacactc gatgaagaag gaacactcga tgaattgcgt catttgactc
      721 gttcgaccat cactatagaa acagaagcag agaatagtca cttagcacaa ttgaacggtg
      781 tccatgactt tgtgcaaaaa ggtcacctcg ctcaatttgc tgctgataac gaagctttcg
      841 atgtgatttt agctgaagcg tccaaattag gtgtgaaaaa atttgaatct attccaccga
      901 ctcttgagga tttattcatg agacattatg aaggatgagg aggaagcgaa atggctgata
      961 aattttcacg ctggtccacc cttttttctc aaaaccttag ccgtgactgg aaaaagatcc
     1021 tgatatggct agtcggtgtc actgcttttt ctggtggttt tgtccctgcg tttgaagaat
     1081 tgacttccgg acaaggatta gctggattat acgaaacctt gcaaaacccg gcaatgattg
     1141 caatggtcgg accaactcca gcttcgaatg ctgctgagta tactttagga gcgatgtatg
     1201 caaatgaaat gttgcttttt tgcagtttgt ttgctgctgt gatctctgtt ttacacgtgg
     1261 tgagccaaac gagaaaaaaa gaagaacttg gtctgattga acttattcgt tcctttcgag
     1321 tcggacgtca agcaaactct ttagctgtca tcgctgaaag tattctgatc aatttgcttt
     1381 tagcaatatc gatcagctgt acgatgattt ttttccaagc ggattcgatc actgcagaag
     1441 gtgcttggct ttttgggcta tctgtaggta gtatggggat tttggggagt gccgttgcac
     1501 tgtttgctgc acaactgatg ccaagttcag ccagttcgaa tggtctgtct ttagcgtttt
     1561 taggtggact ttaccttcta cgagcaataa cagatgtagc tgattcctcc ctttcgatgt
     1621 ggaatccttt aggctggtct tatttgacct atccatttac aactaacaac tggattcctt
     1681 tgctttattt gtttttgttc agtctaatct tgataatcag ttcatttgtt ttagaggaac
     1741 atcgagattt gaacaccggc tatatccccg aacgtgaagg aaaaccgtat gctaaacgtt
     1801 ctcttttatc cttgcgtggt ttattattct atctaaataa agggatgaca atcagttggc
     1861 ttatcacttt tggtttgatg ggggcggcat acggctctat ctacgggaat atgcaacagt
     1921 ttttagaaag taacgactta atgaaacaga tgtttatcca atctggcgtc tcaattgaaa
     1981 aaaactttac aagcacgatc atggtagtct tgatttgtct agctgctatt ttaccaattg
     2041 ctagcatcaa tcgtttattt acagaagaaa cccacggacg tatgaatcag ctgttcgcaa
     2101 caaaaatttc taggaaccaa ctttattgga ccaatatgtt actagctatt ctatttgcta
     2161 tattggcaat cctgattagt tctggtgcac tagggctttc tgctttggct tcgatgaagg
     2221 atgcgaaaga catggatcta ttgatgtttc ttaaagccgg gttcaatttc ctgcctgccg
     2281 ttttagtgtt tatcggaatt agtggctttc tattcggatg gcttcctcga atcggtaaac
     2341 ttgcttatat ctacttagct tactcctttg cactcaatta ctttggcgga attttggatt
     2401 taccagaatg gttttccaaa acagctgtcc ctagctgggt tccacggatg ccaaacgatt
     2461 cttttgatct ttccgtgttc cttacgttat catgtatcag ccttgcactg ttcgtccttg
     2521 gttctattgg ttataggcga agagacttac tagaaggata ataaaaaagc atgtacaatt
     2581 tcatgctagc ctccaaatct gggtcaaaaa tccaacattt ggaggtcatt tttttattta
     2641 caagtcttct aactgctctt tatcaatgat ccagacttct cgattctttc tttttacata
     2701 accattctcc tctaataatt taaattttct agataaagtt tcgggggtcg taccgataaa
     2761 tgcagcaagt tctttcattt tcattggcag gaaaaagtga tccttccctg tcgctttagc
     2821 taagtcaagc agatagacag cgactcgttc ttctactttt tccatcatca aaaaagttgt
     2881 ctgctgctgt acttttgtta atttctcaac gttgattgct aaaagtttca gactaagggc
     2941 gggataatgc agtaagagtt ccgagaaatc tttttgtctc aatatgcaaa tttccgtttt
     3001 ttctagtgct tcgccaaata aagactcatt tggaacgcct agcaactggc tctctccctc
     3061 atatccgccc ggctctatta ctcgtaaaag ctgttccttt ccgctagcag ataattgata
     3121 cactttcata ctgccggagg ccacgatcac taattgaggt tctccattcg gcgcaagaat
     3181 ctgttctcct ttttccacat agtgatgttt gacaagtgcg tgtacttttt cttgatcttg
     3241 ctgtactaag tgattgaaca aaggaacaaa tgaaacacat aaatgatcat gactcataat
     3301 ctctcctcct tctaaacagc tatttctaat aaagaaacat aacttccctt attccaactg
     3361 ataattttct tttatcatag cataaacgtc aagcgaacca gataccagca aatagagtgg
     3421 atttcttttt gtcccttgct ttattttcct tcttttgaga ccttaagtaa ttgattccta
     3481 tcaatttttt aaatatttac ttttt
//
LOCUS       DAYYGO010000177         3456 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000177 DAYYGO010000000
VERSION     DAYYGO010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.184"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            101..409
                     /locus_tag="ACHA5T_002703"
     CDS             101..409
                     /locus_tag="ACHA5T_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFM8888224.1"
                     /translation="MFQNKGQRYVTKGVMDSLLVELQTLCWNLIDQNVQKKLPLDYLQ
                     IFEFSIEKGNQKLVHRQEEPEARKEYLISPKLRLESVSQKIWVIDSGEYQTMLLPEEY
                     "
     gene            436..735
                     /locus_tag="ACHA5T_002704"
     CDS             436..735
                     /locus_tag="ACHA5T_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HFM8888225.1"
                     /translation="MLETAIEVLEKCAQLVTTPEEWGYESVTMEKEEIEMGTLPKNVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVCGLLVEGRLVWSQIGGTNE"
     gene            909..1454
                     /locus_tag="ACHA5T_002705"
     CDS             909..1454
                     /locus_tag="ACHA5T_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HFM8888226.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEEKEKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFSRGNDEQSLE
                     ILTTIFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLSDHSLLFQ"
     gene            1610..1906
                     /locus_tag="ACHA5T_002706"
     CDS             1610..1906
                     /locus_tag="ACHA5T_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888227.1"
                     /translation="MKVDGECLTSSYKSIYEFFEEKGLDTDLLYQFYIAFRGQQLSFP
                     MKMYDRELVAQKIETMIQENHSIDSRKLTDRYGFSTRWIQSIIRQQQKEQHVHG"
     gene            1899..2789
                     /locus_tag="ACHA5T_002707"
     CDS             1899..2789
                     /locus_tag="ACHA5T_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HFM8888228.1"
                     /translation="MANEETVGLRIIEEAFKLPMVKVDRRAFLIQTFQGNVSDIEGLI
                     EKGPQAFLSKEMLDREAQKCIQSITLKSSAVSLATGLPGGIAMAATIPADLLQFFGYS
                     LRLAQEISYIYGFKDLMGETGEFSEEAKNTLILYLGIMLGVTSAGSAVRAMSGKLSTQ
                     ALKKIPQKALTQTLYYPVIKRVLGIFGTKLTKNTFAKGISKAVPVVGGVVSGGINYLS
                     MKPMATKLQTELGKNVAYTEKNLQQDLDILEGEFEEVSSDIVSSDTVLSQLERLSHLL
                     ETNMITEEEFQQLKQELLKK"
     gene            2871..>3456
                     /locus_tag="ACHA5T_002708"
     CDS             2871..>3456
                     /locus_tag="ACHA5T_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFM8888229.1"
                     /translation="MKAKRLFLLGTILLLGGCSTVSQNEQKKQVDSTTYETETTRKES
                     STKEIETTTQETKTTESSSKATSSDTSDFQIKQELWDTNKASQLATFVPQWGKTLGQE
                     YKSYNPQNNVSLYGTPLPSAVIDGNWKMAINEAPVTVQWSEDGTGNAGFNLVAVYSDV
                     ETGEYLGQHVYFFGFQNGQPKVYLTQQNQGNENNY"
BASE COUNT         1261 a          502 c          697 g          996 t
ORIGIN      
        1 caatgaaata gagataaatt caaatatata ttatatccat gaagtccaat cttctttata
       61 gaagattggg ctatttttta cgacaaagga gggaaaagaa atgtttcaaa ataaaggaca
      121 acgttatgtc acaaagggag taatggacag tttactggtg gaattacaaa ctttatgttg
      181 gaatttaatc gatcaaaacg ttcaaaagaa gcttccttta gactatcttc aaatctttga
      241 attttcgata gaaaaaggaa atcaaaagtt ggttcatcgt caagaagaac cggaagcaag
      301 aaaagagtat ctgatttcac ctaagcttcg tttggaatca gtgtctcaaa aaatatgggt
      361 cattgattcg ggtgagtatc aaacgatgtt attaccagaa gaatactgat atctaaaaga
      421 aaggagcgaa atgacttgtt agaaacggct atcgaagtac tggaaaagtg tgcacagcta
      481 gtcactactc ctgaagagtg gggctatgaa tccgttacga tggaaaaaga ggaaattgaa
      541 atgggtacgc ttcctaaaaa tgttcatcta ccacgtttag tgatgacaca tctttatatt
      601 tattgcgcac cagaagatgg aaaagactat gtagtttatt tcattacaga tattaccagc
      661 cagcgagaat ttgtttgtgg gttactggta gaaggacgat tggtttggag tcagattgga
      721 ggaacgaatg aataagatcg attatttgag aatgattgcg aaatgtttag aagatatcct
      781 taccgcgatt tatcgaattc tgaagaaaaa aggagaaaga atatgaaata ttttcaacta
      841 gtattgacac ttataacggc aatggtaatt gaattgcttg aacaaaaatt agcataggag
      901 aaaaagagat gattgaaaaa gaattagtga ccacacaaac agaagtttgg cgagatcctc
      961 atcataccca tcgctatgtt ttcaaacgac aatgggcaat caaaggaaaa gaagagaaag
     1021 aaaaattagc agtagtcatt accattcgtc caacagatac agaaccattt acgaatgatt
     1081 tgagtatgtt attaatcgaa aagaatatcc gacaattggg atttactggt tttattgccg
     1141 tgaatctttt ttcgtataca aaagcgaaaa gtcctcgggt attttctcgt ggcaatgatg
     1201 aacaatcttt ggaaatatta acgaccattt ttacagaaaa gaaaatcaac actattattt
     1261 ttgcttgtgg ttcgattacg gcaacgagtc aagtggctta tgaacaagca aagaccattt
     1321 acgatcaatt gagtgcgaaa caaaagaaga tgaccaaagt attgataaat gcagaaggca
     1381 agttatctca cccattaagt attcatgttc gtaatgaatg gcaattgtcg gatcattctt
     1441 tgttatttca ataaacgaaa ataagtattc aatgtatatg tttctttttc tgttatttta
     1501 cttgttcttt tgcagactaa acaacttgta aaacctcttg ttatatgcat tatcaatgaa
     1561 ggaatcaata tgatattttt aaaatgtcag gggaaatgga ggtgagcaag tgaaagtaga
     1621 tggggaatgt ttgacgagtt cgtataaaag tatctatgaa ttttttgagg aaaaaggact
     1681 agacacggat ttactttatc agttttatat cgcttttcgt gggcaacagt tgtcgtttcc
     1741 aatgaaaatg tatgaccgag aattagtcgc tcaaaaaatt gaaacaatga tacaagaaaa
     1801 ccattccatt gattctagga aattaacgga tcggtatggt ttttcaacga gatggattca
     1861 aagtattatc agacaacaac aaaaggagca acatgtacat ggctaatgaa gaaacagtag
     1921 gtttgagaat catagaagaa gcattcaaat taccaatggt caaagttgat cgtcgagcat
     1981 ttttgattca aacatttcaa gggaatgttt ctgatattga aggactcata gaaaagggtc
     2041 cacaagcatt tttatccaaa gaaatgctag atcgtgaagc acaaaaatgt attcaatcga
     2101 ttaccttgaa atcaagtgcg gtatcgttag caactggact gccaggaggt atcgctatgg
     2161 cagcaactat tccagcggat ctccttcagt tctttggtta ttcattacga ttagcacagg
     2221 aaatttcgta tatttatgga tttaaggatt taatgggaga gactggagag ttcagtgaag
     2281 aagcaaagaa tacgctgata ttgtatttgg gaattatgct tggagtaact tctgcaggtt
     2341 ctgccgtacg agcgatgtct ggaaagctgt ctacacaagc attgaaaaag attcctcaaa
     2401 aagcattgac acaaacgctg tattatccag taatcaaacg agtactaggt atttttggta
     2461 caaagttaac taaaaatacc tttgcgaaag gtatttctaa agcagtacca gttgtgggag
     2521 gcgttgtttc aggaggcatc aattacttgt ctatgaaacc aatggcaacg aagcttcaaa
     2581 cagagttagg gaaaaatgta gcgtatacag aaaaaaatct ccaacaagat ttagatattt
     2641 tagaaggtga atttgaagaa gtatcttctg atatcgtgtc ttctgataca gttctttctc
     2701 aattagaaag actttctcac ttgttggaaa cgaatatgat tacagaagaa gagtttcaac
     2761 aattgaagca agagctgtta aagaaataat tatttagaaa aagcaaccac ttatacgggt
     2821 ggttcttttt cttgaatgaa ttcaaattta ttattaggag aagaaaaagg atgaaagcaa
     2881 aacgattatt cctattaggc acgattcttt tacttggagg atgttctact gtctctcaaa
     2941 atgaacaaaa gaaacaagtg gattcaacaa cttatgaaac tgaaacaaca agaaaggaat
     3001 cttctacaaa agaaatagaa acaactactc aagaaactaa aacaacagag tcttctagta
     3061 aggcaacgag ttcagatacg agtgattttc aaataaaaca agaattatgg gatacaaata
     3121 aagcatctca attagcaact tttgtcccac aatggggaaa gacattaggt caagaatata
     3181 aaagctacaa tcctcagaac aatgtaagtc tgtatggtac gcctttacca agtgcagtta
     3241 ttgatggaaa ttggaaaatg gcaatcaatg aagcaccagt tactgtacaa tggtcagaag
     3301 atggaactgg aaatgcagga tttaatttag tagcagttta ttctgatgta gaaactggag
     3361 aatatttagg gcagcatgtt tactttttcg gttttcaaaa tggacaacct aaagtttatt
     3421 taacgcaaca aaatcaaggt aatgaaaata attatc
//
LOCUS       DAYYGO010000178         3446 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000178 DAYYGO010000000
VERSION     DAYYGO010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.185"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            17..1756
                     /gene="yfcC"
                     /locus_tag="ACHA5T_002709"
     CDS             17..1756
                     /gene="yfcC"
                     /locus_tag="ACHA5T_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFM8888230.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            1913..3097
                     /gene="iadA"
                     /locus_tag="ACHA5T_002710"
     CDS             1913..3097
                     /gene="iadA"
                     /locus_tag="ACHA5T_002710"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFM8888231.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3208..3411
                     /locus_tag="ACHA5T_002711"
     CDS             3208..3411
                     /locus_tag="ACHA5T_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFM8888232.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
BASE COUNT         1091 a          581 c          812 g          962 t
ORIGIN      
        1 aaaggaagaa ataaatttga agaaaacaaa ttcttcagga aaaccgaaac gcgacttcac
       61 taaattatcg acaccccata cgtatgtcat tatttttggt gtagtcatct ttgcatggat
      121 actgactttt gtcgttccag caggaaagtt cagcacgcaa gatattgaat acaaagatgc
      181 aaatggcgaa acaagtactc gtactgtttt acgtcaagat tccttccgtt atgcttacga
      241 gctggataag tcgtatgtgt ttgatcaatt agaagaactg caagatcatc cagcagaacg
      301 tgaaaaactg gatgttccgg aaaaaggatt ggaaaaagtc attgctgacg gagaaaaaaa
      361 tcttacgcag gaaaaactag atgaaatctc cttgacagat gatgtgcttt acgatcagta
      421 cggcgaaaat atctatgata cttctaaaaa attgcataaa acagccaaaa tatggggaac
      481 agatgatttt ggcggctttg gtttcttaaa ttttgttttt gaaggactag tatctggaga
      541 taagtatgga tctgctgtag ggatcgctgc attgatcctt gttgttggag gtgcttttgg
      601 tattattatg cgtaccggag cgatcgacgc aggtatctat gcgtttatca gtaaaactaa
      661 gggactggag cgtttagctt taccgctatt attctttgct ttttcattcg gtggcgctac
      721 attcgggatg gcagaagagg taattccatt ctcaatggtg atggttccat ttgtcattgc
      781 gttaggatat gactcgatcg tagcagtaac cgtgacttat gtcgcatctc aagtcggaaa
      841 tgcgacctct tggatgagcc cgtttagcgt agcggtggct caagggattg ctggaatccc
      901 cgtgttgtct ggtgcgacat tccgattgat tatgtgggta gttgttactg cattagccgc
      961 tggttatatg atgatatatg cagaaaaaat ccgtaaaaaa ccagaaagtt cgttgactta
     1021 caaatcagat gattatttcc gttctcatat caaaaagaca tctgatgaaa ataaaaaatt
     1081 catgcttggg cataaattga tcttattaga gatgttagtt gtccttgtct ggatcgtttg
     1141 gggcgtgact caaaaaggct attacattcc agaaatcgct tctcaattct ttgtgatggg
     1201 attagttgct ggaatcattg ctgttctttt caaattagat ggaatgaaag tcaatgacat
     1261 cgcttcttct ttccaatctg gtgcagctga tttagctggt acagcaatcg tagtaggtat
     1321 ggccaaaggg attcttcttg tcttgggagg atctgatgca tctgttcctt cagctttgaa
     1381 tacgattttg catgggatcg ggacggcgtt gaccggtgtt ccagctgtca ttggtgcttg
     1441 ggcgatgtac atcttccaaa gtttgttcaa cttggttgtt acatcaaatt caggacaagc
     1501 agctttgact atgccaatca tggctccact ggcagattta gtaggtgtat ccagacaagt
     1561 agcagttctt gcttatcagc ttggttcagg ttttatggac gcttttacac ccgtatcagc
     1621 cagcttgatt ggtgtgctag gtgttgcacg gatcgaatgg gcaaaatggg caagattcca
     1681 aatcaagatg caaggattcc tgtttgtttt aggaactatt ttcatcatga ttgccattgc
     1741 aataggtctg cagtagtact ggagacctgt aaataaaaaa gtaatccaaa cagtagataa
     1801 ggactccaag aaccgtcatt ttacggtaaa atcaaaggag taaagatcct atcgtttgga
     1861 ttatttttta agatcctatc atcagttata cttaaagaaa gaggaaaaat gtatgaaaat
     1921 cattcgacaa attgaagtgt ttgcaccaga ttacttagga aaaatggatg tactgattgc
     1981 cggtgggaag attttggctg tagaagaaca gctagaaggc ggctacgaag gagtggaagt
     2041 agaagaactt tcaggtgagg gaaaagtact aaccccgggt ttcattgact gtcatttcca
     2101 tattttaggt ggaggaggag aaggcggtta tcaaaatcga acgccagaag tcacattgag
     2161 ccaattgact acagctggtg taacgacagt tgtcggttgt ttgggaacag acggagaagg
     2221 tagagatatg actgcgttga tcagcaaagc gaaaggtctg gaagcagaag gaatctccac
     2281 ttatgtatac gaaggttcgt accgattgcc ggtaaaaaca gtaacagatt ctatcatcaa
     2341 agactttttg acaattgata aaattatcgg aatcggagaa atcgcagtat ccgatcaccg
     2401 ttcttctcag ccaagttttg aagagttcgc tcgtgcagta gcagatgctc gtgtaggagg
     2461 catgttatcc ggaaaagctg ggatcatcaa tgtccactta ggcggaggaa aaagaaagct
     2521 agagctattg acacgaatcg tagaagaaac agaaattccg atcacacaat ttttgcctac
     2581 tcatgcaaat cgcacaccag aattatttga agcttgcgtg gcatttgcca aacgtggtgg
     2641 cacgattgac ttcactgcta gcgaagatcc tgatttttgg gagaagacag atggtgaagt
     2701 tcgcttcagc aaagcactga aacgattgat cgaggaagat gtttctttag ataatttcac
     2761 gatgacctca gatggacaag gaagcttgcc ttattttgat gaaaacaatc atttcctagg
     2821 gttaggagta ggaagtgcca aggcattgct agtagggatc aaagaagctg tgcaaaaaga
     2881 aagtatccct ttggaaatcg ccttacgagc tataaccagt aatccagcac gtatattgaa
     2941 attggataaa aaaggaaaaa tcgaaattgg tgctgatgca gatctgtgta tcttggataa
     3001 agaaacgctt gatattgata cagttatcgc taaaggagaa ataatggtcc aagaaaaaga
     3061 agtcaaagta tgggggactt tcgaaaaaag tttttaatca acagctctaa ctatcttttt
     3121 tagaaaacga gcgttctgtt tgtctgtatc tgttgttgat gttaccctga aggtagatca
     3181 ataaacaaac taagaggagg aagcacaatg gcagatttaa aaggacgttc taaagatatg
     3241 aaagataaag tagtcggtga aggaaaagaa gcatacggca aagcaacaga cgacaagagc
     3301 aaagaaacag aagggaaagc acaatcacta ggtgctgaca tcaaagaaaa agctcgaaat
     3361 ttaggcgacg aagtcaaaga caagttttct cgaaaagacg aaaaaaaata aaaaccactt
     3421 attactaatt gaatcaagca aataaa
//
LOCUS       DAYYGO010000179         3365 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000179 DAYYGO010000000
VERSION     DAYYGO010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.186"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            45..845
                     /locus_tag="ACHA5T_002712"
     CDS             45..845
                     /locus_tag="ACHA5T_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFM8888233.1"
                     /translation="METIIQIMKKYDFSEMETRVYTTLLEKGNLTGYEVSKISGVPRS
                     KVYNILEKLLKKNLIVVNKSEPKLYHAISANEFLEKLEKSVKNDLSFLTKNLGMIKEK
                     DEEEMLWKVDGIEYVLDKVEHLVKNAKESLLIQVWHENFTDSLLKALQQAEKRVDKFV
                     LILFSSTHEYDLPLEKYYIHGFETDKLADFGARWINIVADEQEVVFGTINEELQSTDV
                     TWTKNHAMVNLAKEYVKHDAYTLKVIAESPEELKAKYGEGFEGIRKIY"
     gene            861..1739
                     /locus_tag="ACHA5T_002713"
     CDS             861..1739
                     /locus_tag="ACHA5T_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HFM8888234.1"
                     /translation="MIRNILLTLIVAINLYFIIYFLRDLFKNKQNFKEEPGDMRLLPF
                     TSFIIFFLSTFGVSDFAIGTVLYPKLKWVSMKKLPGTLNTQCVVPVAVMALSYITAIN
                     VGIKTLAVCIICQIIGAYLGPRFVVKLPEKTIKLFVGIGLVIAALLIFAGQMNWIPSN
                     GTASELYGGKLILAGFLLFVYGALNNIGIGSYALTMVTVYLLGLNPVAAFPIMMGACT
                     FSVPIGSVQFIKFNEYSRKITLFTSTFGVLGVLVAVFLVKSLDTYILKWIVILVLLYS
                     AYTMLGSQLKKVTAAN"
     gene            1752..2732
                     /locus_tag="ACHA5T_002714"
     CDS             1752..2732
                     /locus_tag="ACHA5T_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFM8888235.1"
                     /translation="MLFLKKEDIIKSFSMREAIDADKKALSLYSAGKASVPLRTNIDV
                     PKSNGQSLYMPAYVEGGEGALGVKIVSVYPENIKKNLPSVPATMIVLDPETGMVSACL
                     DGTYLTQLRTGAVQGAATELLAREDAKIGALIGTGGQAQSQLEAMLTVRKLEEVRIFD
                     IDFERASQFAEKMMQQFSVTMRPTKTSQECVEGADIITSVTTSKRATFSAEWVKKGAH
                     INGVGAYTPEMCEIPREIIKAADVVIFDTMDGVLKEAGDFISPLQDGYIQRDSYHGEL
                     GQLINEELIGRTSGEQITIFKTVGSAVLDVVVATEIVKKAKENNLGKLIF"
     gene            3003..3095
                     /locus_tag="ACHA5T_002715"
     CDS             3003..3095
                     /locus_tag="ACHA5T_002715"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8888236.1"
                     /translation="MPRRSFDEIFKITAVKLVTEEGFSVKEVSL"
BASE COUNT         1155 a          555 c          709 g          946 t
ORIGIN      
        1 gttgttaccg tagtagctac taaaaataaa gtgaggatta atcaatggaa acgattattc
       61 aaatcatgaa aaaatatgat ttttcagaga tggagacaag agtgtatacc acactattag
      121 aaaaaggtaa tcttacagga tatgaagtaa gtaaaatctc aggtgtgcca cgctcgaaag
      181 tatataatat tttggaaaaa ctattaaaaa agaatttgat tgtcgttaat aaatcagaac
      241 ctaaacttta tcatgccatc tcggcaaatg agtttttaga gaaattagaa aaaagtgtta
      301 aaaatgatct ttcttttctt accaagaatc tagggatgat caaagaaaaa gatgaagaag
      361 aaatgctgtg gaaagttgat ggtatcgagt atgttctgga taaagtagag catttggtga
      421 aaaacgcaaa agaaagtttg ctcattcaag tttggcatga gaatttcacg gattcactac
      481 taaaggcatt gcagcaagct gaaaaaagag ttgataagtt tgtactgatt ttattcagtt
      541 ctacccatga atatgatctt cccttagaaa agtattatat tcatggtttt gaaacagaca
      601 aacttgctga ctttggagca agatggatca acatcgtcgc cgatgagcaa gaagtcgtgt
      661 ttggtaccat taacgaagaa ctgcaatcaa ccgatgtcac ttggacaaaa aaccacgcca
      721 tggtcaatct agcaaaagaa tatgtcaaac atgatgcata tacactaaaa gtgattgcag
      781 aatctcctga agaacttaaa gcaaaatatg gtgagggctt tgagggaatc agaaagattt
      841 actagtagga ggaatttttt atgattcgga atattttgct gacactaatt gtcgcaatca
      901 atctatattt tattatttac tttctaagag accttttcaa aaacaaacaa aatttcaaag
      961 aagaaccagg ggatatgcgt ctgttgccct tcacgtcatt tatcatcttc tttctttcaa
     1021 catttggcgt ttccgacttt gctatcggaa ccgtccttta tccaaagttg aaatgggtca
     1081 gcatgaaaaa actacccgga acgttgaata cccaatgtgt agtaccggta gccgtcatgg
     1141 cattgtctta catcactgcc atcaacgtag ggatcaagac actagctgtt tgtattatct
     1201 gccaaattat cggtgcatat ttaggaccaa gatttgtcgt gaagttacca gagaaaacga
     1261 tcaaactgtt tgtcggcata ggattggtca ttgctgcatt gttgattttt gcaggacaaa
     1321 tgaactggat tccatcaaac ggaacagcca gtgaattgta tggcggaaaa ttaatcttgg
     1381 caggattttt actctttgtc tatggtgcat tgaacaatat cggcattggt tcttatgcgt
     1441 taacgatggt aactgtctac ttgctaggct tgaatccagt tgcagccttt cctattatga
     1501 tgggggcttg taccttctca gtaccgatcg gcagtgttca attcattaaa tttaatgaat
     1561 acagcagaaa aatcacactt tttacttcaa ccttcggtgt attgggggta ttggtcgctg
     1621 tattcctagt caaatcttta gatacctata tcctgaaatg gatcgtgatt ttggtcttac
     1681 tatacagtgc atatacgatg ttgggcagtc aattaaaaaa agttacagca gctaattaag
     1741 gagcgattca aatgttattt cttaaaaaag aagatattat caaaagtttt tcaatgagag
     1801 aagccatcga tgcggacaaa aaagcactgt cattgtattc agcaggaaag gcaagtgtgc
     1861 ctcttcgcac caatatcgat gtgccaaaaa gcaacggaca aagcttatat atgcctgctt
     1921 atgtagaagg tggcgaaggc gcgctaggtg tcaaaatcgt ttccgtgtat cctgaaaaca
     1981 tcaagaaaaa cttaccaagt gtaccagcaa cgatgatcgt attggatcca gaaacgggta
     2041 tggtatccgc ttgtttggat ggtacttatt tgacacaatt aagaacaggc gctgttcaag
     2101 gagcagcaac agaacttctt gcaagagaag atgctaaaat tggcgcgctg attggaaccg
     2161 gcggacaagc acagtcacaa ttagaagcaa tgttgaccgt tcgtaaatta gaagaagttc
     2221 ggatttttga tattgatttt gagcgtgcca gccaatttgc tgaaaaaatg atgcaacaat
     2281 tttctgtaac gatgcgccca acaaaaacca gtcaagaatg cgttgaagga gcagacatca
     2341 ttacaagtgt gaccacttca aaacgtgcga cattctctgc tgagtgggtc aaaaaaggtg
     2401 cgcacatcaa tggagtcggt gcttacacac cggaaatgtg tgaaattcca cgagaaataa
     2461 tcaaagccgc agatgttgtt atttttgata ccatggacgg tgttctaaaa gaagctgggg
     2521 acttcatttc accgctgcaa gatgggtata ttcaaagaga cagctatcat ggggaactag
     2581 gccaactgat caatgaagaa ttgatcggtc ggacaagtgg agaacaaatc acgatcttca
     2641 aaacagttgg ttcggctgta ttagatgtgg tagttgcgac agaaatcgtt aagaaagcta
     2701 aagaaaataa tttgggaaaa ttgattttct aattaaaaag gctgaaatta aaacctttat
     2761 tattgcgttt aaaaaaagaa aacaaagaag tttgtaatca aatggaaact gaccccataa
     2821 tttaaataaa ataaaaaaca tctcttgttg aattctagta atgaagcgac cctaaaactt
     2881 agaccaaaaa atctaacttt tgggtggagt tgtcaagaaa aaccgggcac ttagttaaaa
     2941 aataagttgg acgggttata ctaaaattga caccaaatta agagaaagcg aagatctgga
     3001 ttatgccaag acgttcattt gatgaaatat ttaaaattac cgctgtaaaa ctggtaacgg
     3061 aagaagggtt ttctgttaaa gaagttagtc tatagctaga agctcgctct aatagcgtgt
     3121 attgatgagt tcaagaagtt gaaaaatatg gataacgtac ttttctcggt agaggaagtc
     3181 atgaaatcca ttcgcagaaa tgaaattaca cgcttagaaa aagagaataa acggttaaaa
     3241 gaataggtag aactcctaaa aattaccgaa gtcttcctga aagaaaaggc tcttcgtcaa
     3301 attgtgttgg cgaagacaaa tttgaataaa acttagtccg atatgcggcg cgttctgtgc
     3361 gccgc
//
LOCUS       DAYYGO010000180         3231 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000180 DAYYGO010000000
VERSION     DAYYGO010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.187"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..340)
                     /locus_tag="ACHA5T_002716"
     CDS             complement(<1..340)
                     /locus_tag="ACHA5T_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008177227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8888237.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVSQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYSFEEKIP
                     HFSTFGKNYVR"
     gene            992..1834
                     /locus_tag="ACHA5T_002717"
                     /pseudo
     CDS             992..1834
                     /locus_tag="ACHA5T_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
     gene            1956..>3231
                     /locus_tag="ACHA5T_002718"
     CDS             1956..>3231
                     /locus_tag="ACHA5T_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM8888238.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHG"
BASE COUNT         1007 a          593 c          642 g          989 t
ORIGIN      
        1 ggcgaacata attctttcca aaagttgaga aatgggggat tttctcctcg aaggagtagc
       61 ctaaaaacca gcggtaagcg acattggtat cgacttcctt aatggtttgt cgcatggaac
      121 ggataccgaa taaatactga ataaacacca atttgatcag aatcactgga tcgatacttg
      181 ggcgacctaa atcggagtaa gtggcttcga cgattggata aataaattca aaatcaagcg
      241 cggcatcaat ctttcgaacg agatgatctt gagaaactag atcatcaatc gtaagcatcg
      301 caaattgatt tctaccattc gtattgtttt tagacatcat agctatcact ccttgcattc
      361 gttttcataa atctattata aaagaaaaga ctgtagacat catccataaa atggactttg
      421 tctacagtct ggataacgtg attttttgct gtaatgacaa ggttttagct gtttcagata
      481 ccatgtatcc aaaaatagtt tattgagaaa ataggagagt atttgatact aactagtagg
      541 aaaaaatgag ataaaaagta aatggaatca tttaaggtaa tctgaaataa aaaatataca
      601 gccctgtggt actttaccta caaggctgta tatatttttt actctcagcg tttttgactc
      661 cgtgtctgac tccgttttat tgattttgaa tgttattcaa tgaccattta acaggtcaaa
      721 tttgatgttt ttgattccgt atgacgccgt ttgatatcta gtgatatagt gatttgagtc
      781 tacatcgaat aagggaataa accaataaaa aatgatttag aatattaaaa taacaaaaaa
      841 gaggactacc tgtattagtc ctcttttttg accagatttt gaccagtcta ttttacatcc
      901 tagtatattt atatgagcta attttatgat taaatggtaa gtttttcctt gtgtgtttta
      961 gtttaaacta gtgtgtaatt caaaaatcta tatggaagga acagtattaa gaatagaaca
     1021 gggttctcta catgatggtg ctggtttacg gaccgttgtt tatttaaagg gctgcccatt
     1081 acgctgtgcc tggtgttcga ttcccgaatc acaaagcaag cagattgaaa aaggatttgg
     1141 ccagaccatg accgctgaag aggtaatgga tgagattgag aaggatgctg ttttttactt
     1201 tcactcggat ggcggagtga ccattagcgg aggcgaggca ctggttcagg cggattttgc
     1261 taaagaaatt ttgcaaaaat cgaaatacat tggaatcaat accgtactgg aaaccagctt
     1321 ttgtggtgct tataatgaaa tccaaaaggt agctccctat gtggatacat tgtttgtgga
     1381 tgtaaaaatg tttaccagca agctccacaa acaatggacc ggcttagata accaacaaat
     1441 tttgaagaat attcgtcgct ttttaattga gtatcctaac tgcgaagtgc gaattcgagt
     1501 tcctgtggtt ccaggtatca atatgaacct gaccgagctg ctgacgattg cttgttttgt
     1561 ggcagatctt gatcggttcg taccgttaga attactaccg taccactgct atggtatgca
     1621 cggttaccaa gcattgggac tggagtatcc gttggcagac acgccagcgc cttcacctaa
     1681 agaaatgttt gcattggcag atcaactggc tcgcacggtt cctcatttgc tggtcacagc
     1741 tttaaccaaa acatttattt atttattgaa tccaagcttt atcttatgga taaatgaaag
     1801 acactcacta gctgcagcca gctcaagaat agaggctcta tactttttga tgttggtgaa
     1861 cttcaccaac atcttttttg tataaaaaaa tatagaaaag acaccctgaa atcctttaga
     1921 attaatttgg ctaccaattc ataggaggat tcaagatgtc tcatactcag cttatcaaag
     1981 ataccctaaa tattctagac ctaaatatct atttcgagga aaattgttta actaaagaaa
     2041 aatacaaagg gcaaatttgc ttgatttaca aaggaactct tctgtacaag ccagaagagt
     2101 gttcccattg tctttgtgtc gtcccctcta ggatcattcg ttgggggacg acaaccgttc
     2161 ggttgttact gaatgatgtg tctgagtatc gcacttatct ggagctgaag aaacaacgat
     2221 ttaaatgtaa gtcttgccaa agaacattcg tagcggatac ctctgttgct gaaaaacact
     2281 gtttcattag tcaaaaagtt cgttggtctg tcattgctcg cttaaaagag aatacttcca
     2341 tgacggaaat cgctcgtcag aaaaatattt ccacttcttc ggtctatcgt gtcatgaagc
     2401 gattctaccg cccattgaat cctttcaaac agactttgcc aaaggtctta tgctttgatg
     2461 aattcaaatc ggttcgaagt gttgccagtg caatgagttt tatcatgatg gacggacaaa
     2521 gccatgcatt attggatatt gtcgaaaatc gacgcttgcc ttatttagaa cgctattttt
     2581 ctcggttttc attagcgact cgtgaagccg ttcaattgat tgttattgat atgtatgcgc
     2641 cttatgtttc gttggtgaaa aagcttttcc cgaatgctca gttgatcatc gatcgtttcc
     2701 acatcgtcca acatattggc agaactttct tgaatcatcg agtgaaagaa accacggtac
     2761 gacttaaagg aaattcccac tctcaacgag ggttagggaa aaaattgaag cgctattgga
     2821 aactcctaca aaaagaagaa gcaaagttaa actatgaaaa acgggtttgg cgtgccagtt
     2881 ttaaagatta tttgactgaa agtgagattg ccgatcgact gttagctgct tgtcccaact
     2941 tacgccaagg ctatcagtta taccaagaca ttctttatgc ggtaaagaaa agagatcagt
     3001 cactatttga ggattgttta actagagaca ttagcggtct gccagaaacc tatacaacca
     3061 ctttacgaac gtttaaaaag tttctgccac agatccaaaa tgcgcttcac tacagctatt
     3121 caaacggacc attggaatgc ttaaacaacc acatcaaagt attaaaacgc aatgcgtacg
     3181 gctttcgcaa cttttacaat ttcaaactac gaattatgat tcgtcatggc a
//
LOCUS       DAYYGO010000181         3221 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000181 DAYYGO010000000
VERSION     DAYYGO010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.188"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            301..585
                     /locus_tag="ACHA5T_002719"
     CDS             301..585
                     /locus_tag="ACHA5T_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888239.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="ACHA5T_002720"
     CDS             588..1004
                     /locus_tag="ACHA5T_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888240.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="ACHA5T_002721"
     CDS             complement(1141..1860)
                     /locus_tag="ACHA5T_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM8888241.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2544..2630
                     /locus_tag="ACHA5T_002722"
     CDS             2544..2630
                     /locus_tag="ACHA5T_002722"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFM8888242.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2932..>3221)
                     /locus_tag="ACHA5T_002723"
     CDS             complement(2932..>3221)
                     /locus_tag="ACHA5T_002723"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888243.1"
                     /translation="EYRRQRSDFISRKIVRLEKEKMEKFPYEYFQNLEKLEQAIQAIK
                     FDLTDEKLLEYKNMTPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1074 a          599 c          503 g         1045 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtactc
     2041 agataaaaag ttgttagtac ccttctgtat ccgttgggag agtaaggctc aactgccccc
     2101 taaaaagggt tctaaaaaat ctttaaaaaa taaataaaat aaacgcgcgc gcgcgagcaa
     2161 aatttcccgc caaaaaatca cccacttttt aaaataaaca ggttcgcgca tgatattaaa
     2221 ctgccaatct agctccctat ggtcgctgta agaaggagtg atcgctacgc tctcacattt
     2281 tacttgcagc agtcgccttt aggctccttc gccaaaaata attcattagg gactcctttg
     2341 tggcgtcctg agagctcgtc taagccgttc taagacaaga ttcgataaaa actatgtcct
     2401 tcgttctaaa gcgccttaca ggcggtcagg gcaagcccta acaaccccaa ggcaataaaa
     2461 aacttaggct tatttttgct tttttattgt ataattgatt gtcgcctatc cgacagtctt
     2521 gcgactggaa ggaggtgtgt ttcgtgttca ctaactttat ttgctccctt cttgttgggc
     2581 ttttaagcgc tttgttcagt tactggttga ataaccgaaa caagaagtaa aacttaatta
     2641 aggcgactca gcccacaaaa aaagccccag agaatccgac ctctctgggg ctttcgtgtg
     2701 tttcgtgttc acttatttgc aatcaaatta taacagaaat aaatatttat ttcaaaaata
     2761 tcctacaaaa gtctaaaatc cgtaattatt tcttatatgc aacatttaca aataaagtcg
     2821 ctctgagagc tcgtattttt cgatttaaga cgttttattt agtttttggt gtatttcatt
     2881 ggtccaagct aaaaaactct tagaagcgat tttagagcca tttaaaccca cttaattata
     2941 ttctccatga aattcgctcc atgcgtcaaa agctgcgtct gaaattccgc cgtcttcgtt
     3001 cactagttgc attaattttt ccaacgatcg ttttctttcg ggtgtcatat ttttatattc
     3061 caacaatttt tcgtctgtta aatcgaactt gattgcttga attgcttgct ctaatttttc
     3121 taagttttga aaatattcgt aaggaaattt ttccattttt tctttctcca gtctcacaat
     3181 ttttctagaa ataaaatcac ttctttgccg tctatactct c
//
LOCUS       DAYYGO010000182         3192 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000182 DAYYGO010000000
VERSION     DAYYGO010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.189"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..628
                     /locus_tag="ACHA5T_002724"
     CDS             <1..628
                     /locus_tag="ACHA5T_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFM8888244.1"
                     /translation="NTWNNIKDSFWNIVTGIVNSAENAWTNLKNGVSNAINRVKEIFN
                     SLREVNLFEIGKNIIDGLINGVKEKWNALKDTIKGIAGNIKNWIKGALDIHSPSRWMR
                     DMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTPSVQPVVNGISHLPITQTKQNISASE
                     TKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQMKRDAGAVGGTLYAT"
     gene            618..1358
                     /locus_tag="ACHA5T_002725"
     CDS             618..1358
                     /locus_tag="ACHA5T_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8888245.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            1355..>3192
                     /locus_tag="ACHA5T_002726"
     CDS             1355..>3192
                     /locus_tag="ACHA5T_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFM8888246.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCGIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     VLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSVGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWR"
BASE COUNT         1194 a          388 c          622 g          988 t
ORIGIN      
        1 taatacatgg aataacatta aggactcttt ctggaatatt gttactggaa ttgttaattc
       61 tgctgaaaat gcatggacaa atctaaaaaa tggtgtttca aatgctatta atcgggtaaa
      121 agaaatcttt aattcgttaa gggaagtcaa tttatttgaa attggcaaga acatcattga
      181 tggacttata aatggtgtaa aagaaaagtg gaatgctttg aaagatacta tcaaaggtat
      241 tgcaggtaat atcaaaaatt ggattaaagg agctttagat attcattctc catcaagatg
      301 gatgcgtgat atggttggta aaaatattgt tcaaggtatt atcgttggta ttgataaaga
      361 gcaaagtaag ttagatcaaa ccatgacaaa tttagtaaaa acaccgtctg ttcaacctgt
      421 agtaaatgga ataagtcacc taccaattac acaaacaaag caaaatatat ctgctagtga
      481 aacaaaagaa attcatttac atttaaatgt ttatggagat ttacctgatt caatggttcg
      541 gaagattacc acaaaaatta aaacagaatt gacaagacaa atgaaacgag atgctggtgc
      601 agtaggagga acattatatg caacttaaga gaggacaatt ttttattaat caacattatt
      661 cttctgaatt taatgtgtat attcaaaaca ggcctgcttc tgtttcagct agtcgagtaa
      721 ttgaattgag agagcgagaa ggaaatgatt ctatcattat tgataaaggc tattacaaaa
      781 atgtgactag aaagattgag tgttactata aagcaccttc aattgatatg gtgcaggagt
      841 gggaagatcg aatcactgaa tggttagata tgtcgtctta tagtgatttt attctttact
      901 atgatgaaca atatatttat caagctgtag taatagaagg acctgaattt aaaggaacaa
      961 gaaaaacagg aaatattgtc ccttttgaat ttacagtgag tattcgacca tttaaggaaa
     1021 attacaatgg tagatttact attcgacaaa cagaaacttt tgaaatttat aacccagaga
     1081 agtattcttc aaaaccgctt attaaattga gtggttctgg agatgcttct ttttatatta
     1141 ataaggataa atatgatttg aaatcgttgg acagagaatt atttatagat tctaaattag
     1201 aagaggctta tcgaaaatta gatggtaatt tagaacatca ggatcaggtc accttatttt
     1261 tagattttcc atttttatat cctggaaaaa atgaaattaa atggacgaaa aatattcatt
     1321 catttgaaat aatgcctagg tggtggagaa aagtatgaaa ccaagaatat atagtcctac
     1381 tgaaacagat ttctcaacga atggcttagg aattttaaag gattgtacaa gatgtggaat
     1441 atatgaagta gctaatggaa aatatgaatt agagttggat tatcctttag gaactagatt
     1501 tgatgaatat tttgaaaatg actatcaaat aaaagcaaag ccaaacgatc aagaagagta
     1561 tcatatcttt tttattgatg ataaagatat agatactttt ttaaatacag taactattta
     1621 tgctcagagt cgtacaaatc gacttggaag acgggtagtc actcttgtgg aagtggactc
     1681 taaaactggt caagaagcga tatcaattat tgaaactaaa atggataaaa aatctgacat
     1741 acgactttat tctgatatta cggctgtttc tagcacaatc tttgaagcga gaaatgtttt
     1801 aaattgtatt gctggtgaac aaggatcatt gcttcaatat tggggcggag aaattaaacg
     1861 tgaaccattt aagctttctt tgttaaagcg aagaggtcgc aataatgttg gaacgattcg
     1921 gtatggaaaa gatatgtctg gtttgaaggt caaattagat tggacaggta taaaaacaag
     1981 aattattcca tatgctgatc ctcaaagtga agtaggtacg actaatcgaa tttatggttc
     2041 gccagtagat agtgcctata ttaataacta tcctgatgtc tatacagaac atgttcagtt
     2101 tacagaagaa caaggagtaa aagacattaa gagcttaaat aaaatagcta aaaattactt
     2161 taaaacgatc aatcctggct gtgataagcc taaagtttct attacggttg aatttgataa
     2221 gttgactgat agtgaagaag cgaaagaatt tgcgaagatt agaaattatg gtctgtttga
     2281 tacatttaaa atatatcata aaaaatataa tatttatctt gaatcaaagg ttagtggact
     2341 acaatacgat tctttatctg aaaaaacttt gaaattagaa gcaggagaca ttcaagttgc
     2401 tttttatcag caacaagctg taactattca ggataaatta aaagattatg caacgaataa
     2461 ttatatgagt gattttaatg attatgtttc ttcaatgatt acaggtcaag gcagtgcagg
     2521 aggatatgta gttttatggc caaaagaaaa accttccaac atctttatta tggataatcc
     2581 agatttagaa aaggcaagag aagtactaag aatgaataaa aatgggattg ctttttctaa
     2641 gaaaggatgg cgggggcctt ttaattcagc ttggacatta gatagtatat ttaatgccaa
     2701 ttttatacaa actggattaa tcaaagcaga tatctttcaa aattctttta acaagactgg
     2761 agatgtattg aaattagtta atggactact ccaaatttgg aacaacaaaa agaaaataat
     2821 ggagttaact aaaaaaggaa tggaattttg gaattctaag ggttcaattg gaacgattgg
     2881 aacaactgat tctgttggta atccttttcc tggggcttct actccaaccc ctattgaaga
     2941 taattcttta gttattcgta caaatggaga cggcaaatat attttgattt ctcctaaagt
     3001 aggaaaagga ttagttttat taggaaatgg taaagcaatt tattttggag acttagatgt
     3061 acaaggaaaa cttacagtta aaggaaaaga aataattcct ggacaaggtg gtggtccatc
     3121 gggaggtgga gaatctactg gtgggtatcc tagtgaatta aaaacagatg cagaaaagag
     3181 agcttggaga at
//
LOCUS       DAYYGO010000183         3190 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000183 DAYYGO010000000
VERSION     DAYYGO010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.190"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..175)
                     /locus_tag="ACHA5T_002727"
     CDS             complement(<1..175)
                     /locus_tag="ACHA5T_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFM8888247.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSV"
     gene            333..596
                     /locus_tag="ACHA5T_002728"
     CDS             333..596
                     /locus_tag="ACHA5T_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888248.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            620..808
                     /locus_tag="ACHA5T_002729"
     CDS             620..808
                     /locus_tag="ACHA5T_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888249.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(891..1076)
                     /locus_tag="ACHA5T_002730"
     CDS             complement(891..1076)
                     /locus_tag="ACHA5T_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888250.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            1291..1662
                     /locus_tag="ACHA5T_002731"
     CDS             1291..1662
                     /locus_tag="ACHA5T_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8888251.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(1812..1884)
                     /locus_tag="ACHA5T_002732"
     tRNA            complement(1812..1884)
                     /locus_tag="ACHA5T_002732"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1849..1851),aa:Gln,seq:ctg)
     gene            complement(1930..2358)
                     /locus_tag="ACHA5T_002733"
     CDS             complement(1930..2358)
                     /locus_tag="ACHA5T_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888252.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(2418..3176)
                     /locus_tag="ACHA5T_002734"
     CDS             complement(2418..3176)
                     /locus_tag="ACHA5T_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFM8888253.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT         1060 a          515 c          603 g         1012 t
ORIGIN      
        1 atacagacgt tgtttcccaa tgcctaaaag aaaaaatttg ataagtaaaa tatacagcag
       61 gtaaagaggc cagtaaaact gaaaacttct tttttgtcca ttttatgtaa tagaaaagaa
      121 tgcttatcat aataaaaaaa ggaatataaa aaacaaattg accgatcgcg atcatactat
      181 tgcctccgat aaataaagtc catgatacaa aataacactt tgtgcgaaat ggcgcaagca
      241 gttttgtgga tttatccggt aaaatctgta tcgattttga aattagagga gaaacttgct
      301 ataatcaaag tgaaagaagg agtgggattt tcatggatca tgtagtgtta gaaatcgatt
      361 tgcatttgat caataatatt cgggtgattt actatgttgt aagtaacagt gtcgaacaac
      421 gtgtactgac gaataaaata aatggtattt tagcaaagaa agacgttcac cgattcaata
      481 atggtgaagg aagttattat agtatcccag tagaaaaaat tatttatacg acagtcaaag
      541 tcagagaaga tctagatacc aaaaaagcat atgaacctat ttttacaact tattgattga
      601 cagcagatgg aggaaaaaaa tgaatccata tgaacaactt attcaagatt tgatcgatgg
      661 aaaaatcgaa aagattgatg taaaaagaga agaattaatg gcttttcgag aagcgtggct
      721 gaagttggag aatcgtaaat atgtggtggg aaaagcctca ctgaacggca atgtgactta
      781 tcattacgat ccgacacgtt tattttagta tgaagtattc agaaaagaac caaaggagga
      841 cgatgcagca gcattgtcct cctttgattt tacaaaagtg aaagttttac ttatttgaat
      901 agatttttaa atttgttttt gacactgtcg atatttacat cttttaaaag attttgggct
      961 ttctctttat aaggtcctaa ttgttcttta tgttcttcga taaatcgttt tgcgccatcg
     1021 aaattgccgt tcttaatcat ttccttgact ttttgaaata attcttcttg attcattttg
     1081 aacacatcct ttgctatcaa catgattttt tacatggaca aaacgagaaa aactgattta
     1141 tccacacgtc aataatatca aataacgatc atttttacta aaaaaacact cgattctcgc
     1201 ttctaaagta ggagaaaaat ctccaacttg ggacggatgg aatttcttta actgacccat
     1261 atactgaata tataacaagg aggtagaaag atgaaagtga ttaaatattt gattggactg
     1321 gcggcgatta gtggtatttt gctatttgga ctgaaatatt atactgaagg aagttatgga
     1381 gaaatccctg gtatacttga tcagctaaat ccattagttg aaaaaggaga ggtctatgta
     1441 aagacacaaa aaccagagga agtaaatgaa tatggtacag caaggtatgt gcagaaagct
     1501 gctgatgcca atgggaaaga acgaacggtt gaatttaatg gtctttctgt gttaaaagaa
     1561 aatcattatt tgaaaattag caataaagga gcacacatag aaacgtatga ggaagttcct
     1621 agagaaaaag ttccggagaa agcattagca attattgggt aaatcagttt ttgtattaag
     1681 aatacgcccg attttattat tcataattta attatgtaaa ctaaatacaa gtagtaaaaa
     1741 taatacaata atccccctgc aaaaagattg ttcttttaca gggggttcat tttttcagca
     1801 agcacatcaa attgggatgg cagggttcga acctacaatt gcttgattca gagtcaagtg
     1861 ccttaccagt ttggctacat cccatttatt caacaaaata atcatagcac tttcctaaaa
     1921 aaacgcaagt tatttttctt gatcggatag ttgttgcttt actcgtaaaa ggcggcgtac
     1981 tagtttcttt tctaatccag caggaagggc aatcagttct gctactgaca agacggtgtc
     2041 caataactga tattctttga caggttcatt cataatatga tagatgtttg ctaaacgttc
     2101 aatatattca aagcgagtat actctgttcg agcaattaga aatttcaata aatcggtggc
     2161 tttctcgtac tcttgatctg cgtaataaac aagagattga cgatacaatt tgatttccag
     2221 ttcattgagg gtagcaggtc tgcgacgctc gttccactca ttaatagaga ctttaccttg
     2281 aacatttccc ttgcagtcac ggacaaaaat aatcggaatt tcatttggat ccattttttt
     2341 atgtgtgaat ttttccatta tctacgctcc cttcaaaggt ttggtatctt gagtataagg
     2401 gatttttgtt tgaattacta gcttttctcc aaagttagca tctgtccacc cattgcagtg
     2461 aaaatcgccg aaaaatcgtt gatcggtact gctcgatttt ttacaccata gggatcgttg
     2521 acataaatat tttctttgtc atatccagtg actacacaag catgaacaag aggagtgact
     2581 cttacttctc cattatttgt ggtccaagtc ataaaatcag tttcatctgg gacttcaaaa
     2641 ttgatagtca cagaagtcca gacaggattt cctttagata gtgctttttt gatatcatcg
     2701 agttgagcat cggtttttgg aaaagtgtga taatccgttt ggacgatttg ctgagcaagg
     2761 gaggcaactg ggggaacata gacgaccata gcccagtctc cgccactgat atcaccaaca
     2821 aatccttcat taggatcgcc atgagtccca tctgcattga aaacagggac gtaatccaat
     2881 tgctcagcta actggttttt agttgtttca taaccataaa aagataaaag catagaaagc
     2941 gaagtgactt cacagccatt ttctaaagaa ggagaatcaa actggctctc taatggaaca
     3001 tctagtaaaa cttctgcttt ctcttttgtc tttttcgttt tttgtgttga acttttttgt
     3061 gcagtgttca ttgtatgttc tggagaatgg atatgtaggt aataacttgt accgcttaat
     3121 aaaagagcag ctgtgccccc tagaaacaat agttttttga acgttttttt cttcatttga
     3181 aatctccctt
//
LOCUS       DAYYGO010000184         3160 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000184 DAYYGO010000000
VERSION     DAYYGO010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.191"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            54..623
                     /locus_tag="ACHA5T_002735"
     CDS             54..623
                     /locus_tag="ACHA5T_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8888254.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(801..1241)
                     /locus_tag="ACHA5T_002736"
     CDS             complement(801..1241)
                     /locus_tag="ACHA5T_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFM8888255.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            1399..2163
                     /gene="map"
                     /locus_tag="ACHA5T_002737"
     CDS             1399..2163
                     /gene="map"
                     /locus_tag="ACHA5T_002737"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFM8888256.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            2195..3118
                     /locus_tag="ACHA5T_002738"
     CDS             2195..3118
                     /locus_tag="ACHA5T_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFM8888257.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
BASE COUNT          998 a          544 c          642 g          976 t
ORIGIN      
        1 tgaaattaat caatggtgga agcgttataa aaaaattaag gagattaaaa aatatggcaa
       61 gaaaaaagac gattactaga gaacagattt tggcagctgc ttatgaagta gctgccaccg
      121 atggatttac acgatttact gcgagaaaca tcgcgaacaa aatgaattgt tccacgcaac
      181 cgatttatct tgagtttaaa aatatggatg acttgagaca agctcttttt gagcagatct
      241 atgatcattt acaatatgaa gtgttcactg ttgagcatac agggaatacg attatcgatc
      301 ttgcttttaa ttatatccat tttgcaagaa gagagaagaa gttgtatcgt tcactgtact
      361 tagaagaata tggtggagga aaagagatgc agaatttctc ttatacttac tttgtgaata
      421 cagtgaaacg cgatccagct tatgtgaatc tttcagaaga gcagatcgaa tctttgtata
      481 atgggacatg gatcatagct actgggatcg cagctttgat gtcttcaagc atcatccatc
      541 caactgatga acagattgaa aagatgatcc aagacagtat agatgctatc ttgaaattca
      601 aagaaccaat cgaaatcgat tgaatatact caaaagttcc acaaatcagt aggtaacaag
      661 agatgtgtca aaataaggaa aagttcgttt ttcttacagc gtatgcattt ttaactcccg
      721 aagaactctt ccatgaatgc aatgaataaa agagccatac agattgaata aatctggatg
      781 gctcttttat tgcaagtata ttaaattttt gcgactagtt ctttagcgaa agcttctaaa
      841 ttttcgatat cttcttcttc agcattcaga tccactttaa cattttcggc tccttttgat
      901 gcgcctgttt ttgcaaaagc agcatcgaaa tcatccaccg atttgcagaa ttcatcgtaa
      961 aaagtatcac cagatccgca gacaccgtaa atttttccca atagatccaa ttcttgcaag
     1021 tcttcataaa agtctacgat ctcatcaggc agatcgccat catcgtatgt ataagtagct
     1081 actacgcaaa tatctgcctc ctcgaattca tctgcatcta cttgggtaca ttcgttgatt
     1141 tcgacttcga tatccagatt ttcaaatgct tctgcaacaa tatcggcgat ttcttcagtg
     1201 tttcctgtca tgctagcata tacgattttt gctaaagcca tgatgtgcct cctcgtttat
     1261 aattatttaa caattatgaa ttataccaaa acatcattga aaagaaaata aaacctagag
     1321 acacctgggg aatgtcattt ggctaaatgt atgccgctat gttaaaatga gagggatttg
     1381 taaaaaggag acgaaaatat gattacttta aaatcagcac gtgaaattga aatgatggca
     1441 gaatctggag ctttattagc agatgtacat aaaaatctgc gtgattttat aaaaccagga
     1501 atcacaagtt gggatattga ggtatttgtc agaaactata tcgagagtca tggcggaatt
     1561 gctgcgcaaa ttggctttga agggtatgaa tatgcgactt gtatcagtat aaatgatgaa
     1621 atttgtcatg gattccctcg taaaaagcct ttgaaaaacg gtgatttggt aaaagtcgat
     1681 atgtgtatcg atttgaaagg cggcatttct gattcttgtt gggcctatgt agttggagaa
     1741 tctacacctg agatcgaccg attgatggaa gtaacgaaga aagctttgta catcggtatt
     1801 gagcaagcac aagtagggaa tcggattggt gacatcggtc atgctatcca aacttatgta
     1861 gaaggcgaaa atctagcgat cgttcgtgat tttattggtc atggcgttgg tcctacgatt
     1921 catgaagaac cagcagttcc tcattacgga gaagctggaa aagggttgcg gttgaaagaa
     1981 ggcatggtta tcacgatcga acctatggtt aataccggaa cttggaaaat gaaaatggat
     2041 cctaacggct ggactgcgta tacaagagac ggcggacttt cttgccaata tgaacataca
     2101 ttggctatta ctaaagatgg tccaagaatt ttgacatcac aaggagaaga aggcacttat
     2161 tgatctatcc agtcaatagg tacgggagag gtacatgaaa gtttggaaca aaataaaaca
     2221 aaatgaaaat ctgatgcgct ttattgagac gactcaaaag cgcatcactg attcagaaat
     2281 gagtacgacc tctgtcgtcg ttgcttatta tttactcttg tctttatttc cattgattat
     2341 tgcttttggt aatattttac cgtttctcca catcgatcaa gagactgtac tgacctattt
     2401 acgagaagtg attcctgaga ccgtttacca gtttatcggt cctgcaatca aagacctttt
     2461 gacacaaagt tcaggaggct tgttatccgt ttctgcatta gcggctttat ggtctgcaag
     2521 tcaaagtatc aatgctttgc aaattgcgat gaataaagcg tatggcgtag aaaatagaaa
     2581 aaattttttg atcgtccgtt tcttctcact aattgtcatt gtcttgttca tgattgctat
     2641 tagtggggta actatcgttc ttgggttggg acagctgatt ttggatgcta ttcagccaat
     2701 cgtacaaata cccgtgaact ttatcgatca gttccaaact ttgaagtggc caatcacatt
     2761 ggtagcttta tttgtcatca tgttcttgat ttatttaatt gtccctaatg ctcagttgaa
     2821 gttgaagacg atcattccgg gcacgatctt cgctactgtc ggctggatgc tgttatcaca
     2881 agtgtttagt atctatgcta ggtattttgc aacgaggatc agcggttatc agatcatagg
     2941 aagttttatt gtactcatgc tttggcttaa ttttgctgct acgatcatca ttttgggcgg
     3001 aatcatcaat gcagttgttc aagaatacgt gacgggtcat caaatcaaaa aacgtcgaga
     3061 tccaagccga aaatggttca ataaattgaa aactaaattt tctcataaaa aaaagtagaa
     3121 acaagagttc ttattgtaaa tttatatcag taatttattt
//
LOCUS       DAYYGO010000185         2995 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000185 DAYYGO010000000
VERSION     DAYYGO010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.192"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            380..1015
                     /locus_tag="ACHA5T_002739"
     CDS             380..1015
                     /locus_tag="ACHA5T_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8888258.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            1151..1243
                     /locus_tag="ACHA5T_002740"
                     /pseudo
     CDS             1151..1243
                     /locus_tag="ACHA5T_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323349.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            1357..1992
                     /locus_tag="ACHA5T_002741"
     CDS             1357..1992
                     /locus_tag="ACHA5T_002741"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8888259.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            2099..>2995
                     /gene="mef(H)"
                     /locus_tag="ACHA5T_002742"
                     /gene_synonym="mefH"
     CDS             2099..>2995
                     /gene="mef(H)"
                     /locus_tag="ACHA5T_002742"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
                     /protein_id="HFM8888260.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSV"
BASE COUNT         1018 a          451 c          558 g          968 t
ORIGIN      
        1 tttgtgaaat agtgcccccc tgttagataa cgggggcaca gacaaggctc gcaggctcgc
       61 cagccgacaa agcatttctt ccccttgaca caaggtgtca tggcggtaag cctgagaggc
      121 ttccgccacc gtgtcagaaa tactttgtca gcaagttctg ttttgccttt tctaacaagt
      181 tccatttcaa ccgatattca tatctacgga tttaatcaga taggtatttc tttttgaatg
      241 tttttatatt tcattctatt gtgcttattc gattacaaga acatagtaat tcttatatgc
      301 ttatttgtca atatcttttt ttgcctttac aaatcatatt aagcatgata gacttataat
      361 agcgaagata taacgagata tggagggtta tgcaatgaaa ataagccatt taggaaataa
      421 tatacagacc ataagaaaat ttaggggaat gaaacaacag gaacttgcgg acaaaatcgg
      481 tatcaatatg cagagccttt ccaagattga aagagggttg aactatcctg cctatgaaac
      541 gctggaaaag ataatggaag tgctggacgt tacgcccaat gaattgttgt cgggagaatg
      601 gaagtatgtc aatcaatctg aaaaggaagt ctgtcagttt ttaagagccg aagaacgtct
      661 aaatgcagaa ctgaaacatg gacactatga taacttcttt gacagcgagg aagaatggct
      721 ggaatatgag ttagaaaagt tacgggaata tattacagac tatatcaacg ggaaaagcat
      781 tgaagcgtcc gacctttacc caatcaaaga atttatccag catttgaagt ttcaaaaatt
      841 attagaccgc tatgatgatt tatacagtat ggatatgttt ggggaaagca tagagggaca
      901 taagtataag acaccttatc aagtcgtgaa aatgataaac ccgaactcaa aagaagatat
      961 ggaactgtta cgagaagtat taaggaataa caattttgat gatgaggacg agtaactttt
     1021 ttcctcttgc gagaagatac agcaaacaaa aagcccgatc aagagtgcaa agcaccacca
     1081 atggtggcaa agctcttgac agggcttttt cttttctgtt gtgggcaatc aagaggaaga
     1141 aagggataaa atctgatttg tggtaaatca ggcaaaggca acgcaggtat cacagtatga
     1201 attgacaccg cataaggaaa gcaactggaa gattgtagaa taaatgaaaa aggcttgcat
     1261 tctgcatttt tataaaattg caagtcttta tattgacaag cataaaatac taagatatac
     1321 tttaatcaga ccaacggttt gtattggagg taagttatgg cacgaaataa atatccagaa
     1381 gaaacagtaa aactcatttt agatgtttct acaaaaatgt tttgtgaaaa aggatatgat
     1441 aacacatcat tgcaagatat tatcaatgaa acaaaacttt ctaaaggtgc tatttatcat
     1501 catttcgatt caaaggaaga tattttaaaa gcaatttttc aaagaattgg aaatgaaaat
     1561 gcagtaatat ttgctaaaat tagagataac gatagtttaa acggtttaga aaagttaaga
     1621 gaaatattta agacagcggt attcaattct aaccaaagcg ttttattgac agtttctcct
     1681 caactattaa acaatcctcg ttttcttgct atgcaaatag aacaaatgta tgaaattgta
     1741 gtacctgaat ttgttgaacc cgttttagaa caaggtatca aggataagtc tttggagatt
     1801 gaaaattcac atgaaattgc agaagttatc atggtattaa gtaatgtatg gctcaaccca
     1861 ttagttgaaa tgacggataa agagggtatg gaaaaacgtt gcaatacatt caatgccctt
     1921 ttaaatggaa taggcataga cacattatta gacgaagaaa caatttctgc atttatacag
     1981 ttttgtaaat aactgtattt gttatcccta tacgatagac tgctctataa aagagcagtt
     2041 tatttaaacc attatataaa ccgacggtct gtttgattaa ataaaaagga gagattttat
     2101 gcaagcagaa aaaaaacttt ttaatcgtga ttttatactt gttgttatag gacagataat
     2161 ttctttattt ggaaacgcta tcctacgatt tgcattaccg tactatttgt taaaagaaac
     2221 tggttcatca gctttgtttg gtattgctac agcctgctct tttttaccta tggttatttt
     2281 gtcgctggta ggtggtatta ttgctgacag gacaaataaa agaaacatta tggtcatact
     2341 agattttgta acagcaatca taattttagt attttatttg tcattgggaa aaattcctat
     2401 catacctttg tttattgttg ctttgatgtt actttatggt atttctggtg catatcaacc
     2461 agcggtacaa gcaagtattc ctctattggt atcatcagat aaacttaccg tcggtaatgc
     2521 tataataaat caagtaaata ctttagcaaa ctttatcgga ccagctataa gcggagtagt
     2581 ttttcaattt tatggtatca atcccatttt aattgttagt attatatgtt ttactttttc
     2641 agccattatg gaaattttta tccatattcc acaccaaaaa agacctcgtg aagtaggtgt
     2701 aattgcagtt gctaaaaacg atttaaaaga tagttatacc tttttgaaaa aggagaaacc
     2761 catttttata tccgtaatct ttttggtttg cattttcaat ttggttttaa gtgctgtttt
     2821 aattgttggt ctgcctatct taatttctga agttttaaaa atgccagata cactatatgg
     2881 tttttcacaa tcagcattat cattaggtgg tttatgtggt ggtattttaa cagctcttgt
     2941 tgcttcaaag ttaaaactaa aaaaccttta taccttgtta cttatttgtt ctgtt
//
LOCUS       DAYYGO010000186         2972 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000186 DAYYGO010000000
VERSION     DAYYGO010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.193"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(57..608)
                     /locus_tag="ACHA5T_002743"
     CDS             complement(57..608)
                     /locus_tag="ACHA5T_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HFM8888261.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFLRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            complement(782..1081)
                     /locus_tag="ACHA5T_002744"
     CDS             complement(782..1081)
                     /locus_tag="ACHA5T_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HFM8888262.1"
                     /translation="MLETAIEVLEKCAQLVTASEEWGYESVTMEKEEIEMGMLPEDVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTDE"
     gene            complement(1108..1383)
                     /locus_tag="ACHA5T_002745"
                     /pseudo
     CDS             complement(1108..1383)
                     /locus_tag="ACHA5T_002745"
                     /inference="COORDINATES: protein motif:HMM:NF017894.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
     gene            1721..2404
                     /locus_tag="ACHA5T_002746"
     CDS             1721..2404
                     /locus_tag="ACHA5T_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFM8888263.1"
                     /translation="MTTEHFDLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVLKGIK
                     IGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFAQLSE
                     ALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPILDQVIKNKLAFELNAKSAYLY
                     DNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFDDLFALLKANGVTELAIYQKG
                     KRIMVPLPV"
     gene            complement(2441..2938)
                     /locus_tag="ACHA5T_002747"
     CDS             complement(2441..2938)
                     /locus_tag="ACHA5T_002747"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8888264.1"
                     /translation="MNNQITLAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
BASE COUNT          901 a          561 c          454 g         1056 t
ORIGIN      
        1 ttttaaagtc aaaaagcatg taaaactact tataagtaac atgagaaaat aaaatgttat
       61 ttttcttgaa ataataattg actatctgcc aattgccatt cattacgaac atgaatactt
      121 agtgggtggg ataacttacc ttctgcattt atcaatactt tggtcatctt cttttgtttc
      181 gcactcaatt gatcgtaaat agtctttgct tgttcataag ccacttgact cgtcgctgta
      241 atcgaaccac aagcaaaaat aatggtattg attttctttt ctgtaaaaac agtcgttaat
      301 atttccaaag attgttcatc atttcctcga agaaataccc gaggactttt cgcttttgtg
      361 tacgaaaaaa gattcacagc aataaaacca gtaaatccta attgtcggat attcttttcg
      421 attaataaca tactcaaatc attcgtaaat ggttctgtat ctgttggacg aatagtaatg
      481 actactgcta atttatcttc cgcttctttt cctttgatcg cccattgtcg tttgaaaaca
      541 tagcgatggg tatgatgagg atctcgccaa acttctgttt gtgtggtcac taattctttt
      601 tcaatcatct ctttttctcc tatgctattt tttgttcaag cacttcaatt accattgccg
      661 ttataagtgt caatactagt tgaaaatatt tcatattctt tctccttttt tcttcagaat
      721 ttgatgaatc gcggtaagga tatcttctaa acatttcgca atcattctca aataatcgat
      781 cttattcatc cgttcctcca atctgactcc aaaccaatcg tccttctacc agtaacccac
      841 gaacaaattc tcgctggctg gtaatatctg taatgaaata aactacatag tcttttccat
      901 cttctggtgc gcaataaata taaagatgtg tcatcactaa acgtggtaga tgaacatctt
      961 caggaagcat acccatttca atttcctctt tttccatcgt aacggattca tagccccact
     1021 cttcagaagc agtgaccagc tgtgcacact tttccagtac ttcgatagcc gtttctaaca
     1081 agtcctttcg ttcctttctt ttagatacta gtattcttct ggtaataaca tcgtttgata
     1141 ctcacccgaa tcaatggccc atattttttg agacactgat ttcaaacgaa gcttaggtga
     1201 aatcagatac tcttttcttt cttccggttc ttcttgacga tgaaccaact tttgatttcc
     1261 tttttccgta gaaaattcaa aaatttgaag atagtctaaa ggaaattgct tttgaacgtt
     1321 ttgatcgatt agattccaac ataaagcttg taattccacc ggcaaactgt ccattactcc
     1381 ctttgtgaca taacgttatc ctttattttg aaacatttct tttccctcct ttgccataaa
     1441 aatagcccaa tcttctatta aagaagattg ggcttcatgg atataatata tatttgtaaa
     1501 taaagttaat tcagttataa ttatcaaaac tacacaatta ttaaagtttt tcatatctct
     1561 ttgtttagta agtttttcta aatcttaacc cgtggtgcta gttaattttt tcctagagga
     1621 aaatgtctaa acatgttggt aaatcaacaa aaaaacgcat atagtagtct caaaactccc
     1681 aagaaatgag gactatttaa caaactacga tggttacgtc gtgacaactg aacattttga
     1741 cttagccaat cctgttacta aagtcgatga tatccccgat tatgaaatgt acagtcaaac
     1801 aatcgatagc ttaaataagc gttttggcaa tcgtgtttta aaaggcatca aaatcggtta
     1861 catcgcttct gaaaaggatc ggatcatcga ctatttagcc gataaagatt acgatttaaa
     1921 attactcagt gtgcatcata atgggcaatt tgattattta gatgacgaag tcaaagatat
     1981 ggatcctgct attgtgattc ctcagtattt tgcacaactt tcagaagcac tagttgttat
     2041 agaagcagat gtctttgctc attttgacta tggttttcgg gtatttggtc tatccgttgc
     2101 tgaatttaaa caatatgaag cgcagttttt accgattcta gatcaagtaa tcaaaaataa
     2161 attggcattc gaattaaatg ctaagagcgc ttacttgtat gacaatttag cgttatatga
     2221 atatgtcatc gatttatatc tttcacgtgg tggtaccttg tttagtgttg gatcagatgg
     2281 gcattacctt gaacatttcc gtttccattt cgatgatctt tttgctctgt taaaagcaaa
     2341 tggtgtgacg gaattagcga tttatcaaaa aggaaagcga attatggttc cattaccagt
     2401 ctaaaaaaac gtcctcagca tcaaaagtga ggacgttttt ttagatttct ttttctaaaa
     2461 tcggacaaat acgatcttgt tttcctaaag gaaaatgttt gatcccgatt tttttaaatc
     2521 catgacgttc ataaaaacga atacctgcat gatttttttc ataaacagtc aaacgtaaat
     2581 aacgtttttg tttcgtttta gcataacgat tcgcttcatc gagtaaccgt tgccctaacc
     2641 ctttgttttg ataatctttg agcagataga gccgttgaat ctctatagca tctttttcat
     2701 cattgacttt catataacca atgagtattt gatgatcctt aataccaata aatttactgg
     2761 ttgactgtgc caattctact gccaatattt cagttgtcaa ttgtgtctgt aaatatcctt
     2821 gcatatcaag tggatctgcg ccatcgataa acgattcata aaaactgaca acacttacaa
     2881 cttgtaaagc atctagatca tcataatcta aatacgctag tgtgatttga ttgttcatct
     2941 aactcctcct tttgacatag tatactataa at
//
LOCUS       DAYYGO010000187         2867 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000187 DAYYGO010000000
VERSION     DAYYGO010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.194"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            111..1742
                     /locus_tag="ACHA5T_002748"
     CDS             111..1742
                     /locus_tag="ACHA5T_002748"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFM8888265.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            1745..2824
                     /gene="uxuA"
                     /locus_tag="ACHA5T_002749"
     CDS             1745..2824
                     /gene="uxuA"
                     /locus_tag="ACHA5T_002749"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM8888266.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPACEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
BASE COUNT          989 a          391 c          603 g          884 t
ORIGIN      
        1 atatggtaga aaaaagcaag tttattccct ttttatattg acaacggtta caatgggttt
       61 taaaatataa aatgtatcaa aactaacata tcagtttgag gaggttaatt atggtttcga
      121 ttaaaaattg gcaaaattct atcgaagaat tcaatgaaat gcagattcta ctgccgtgtt
      181 ttgatgtcaa agaagttaag caagaaggcc agcaacatcc agtatggctt cattttggtg
      241 gaggaaatct ttatcgagga tttcatgcag aaattgctca atgcttagca gatcaaaatg
      301 aattagaaac aggagtagtt gtgtgtgaaa catttgatga agaagtagtg gaaaaagctt
      361 atcatgacta taacaatgac attttggaaa ttgttatgag tggagatggt gaactcaaaa
      421 aaagactttt ggcagtaacg gctgatagtt tatattgcaa tccgaagaat atagcggact
      481 atcaaagagt ggttaaatat tttgaagatc ctaaattaca gtttacaacg tttactatta
      541 ctgaaaaagg ttacagttta aaagattcta aagggcaatt cttttcggta gtcaaagctg
      601 atatcaaatc tggacctagc aatcctcaac atacgatgag tattgtaaca tccttgttat
      661 atagcagatt taatgctgga gagttgccga ttgcgatggt ttctactgat aatttctcac
      721 agaatggtca acgttttcaa gatagtattt ttacgattgc agaagcttgg caaaaagaag
      781 gatatgtttc tgaaaaattt attgcttatt taaaggataa agaaaaagtg acttttcctt
      841 ggagtatgat tgatcgaatt acgccaaatc cttcagagaa tgtaaaggaa gatctattga
      901 tgatggggct cgaggattta gatatcattc acacaaaaaa acataccaat attgctgctt
      961 tcactaatac tgaggtagtt cattatttag ttattgaaga tagttttcca aatggccggc
     1021 ctaaattgga aaaggctggt gtgattatga cagatagaga gacggttgat aaagcagaca
     1081 tcatgaaggt tacgacaagc ttgaatccac ttcatactgc tttggcagta acaggttgtc
     1141 tgctaggata tcaatcaatt tcagcagaga tgcaggatga tgatttggtt ggtttaatca
     1201 aaggaatagg ttatttagaa ggtttaccag tagttgaaaa tccaggcatt attgatccaa
     1261 aacaatttat tgatgaagta attaataaac gtttgcctaa tccctatatt ccagatacac
     1321 cacaacgaat tgcaacagat acttctcaaa aagtagcgat tcgctatggt gaaacaatta
     1381 aaaagtatgt cgaaaaagac ggaaatgcag aaaatctaac atttattcct atcgctattg
     1441 ctgcttggtt acgttattta ctggcagtcg atgatcaagg ggaaaatttt gtgccaagtc
     1501 ctgatccgtt attggtagag ttgcaggata aattatcagt attgaaatta ggtgaacaag
     1561 atccttcaaa gattcattgt gcggtacagg aaattttaga aaataaagaa atctttggtt
     1621 tagatttata tcaagttggg gtaggaaaga aaactgaaaa aatcttttgc gaaatgctag
     1681 taggtcctca agcagttcgt aaaacaatca ataaatatat aacagaagtt ggaggaaagt
     1741 aactatggaa atgactttta gatggtatgg ggcaaatgaa gaaaaaatct cactacaaga
     1801 aattcgtcag atcccagcaa tgaaaggaat tgttggaaca ttaatggata taccagccgg
     1861 agaggtttgg ccgagagaac gaattaaatc tttaaaagat gaagtcgaag ccaatggact
     1921 taccttgaaa gttattgaga gtgttaatgt tcatgatgat atcaaaattg gtttaccaac
     1981 acgagatcaa tacattgaga attataaaca gacgattcgc aatttggcag aatttgggat
     2041 tgaggttatc tgttataatt ttatgccagt atttgattgg gtaaaatcgg atttggatta
     2101 tcgtttagat gatgggtctt ctacgttagc ctatatttca aaaaatatcc ctactgatcc
     2161 ggaggaaatt attagagaag tagaaaattc tactaataat tttgaattgc cgggttggga
     2221 acctcatcgg ttatcacaaa ttaagcaatt attagaagcc tataaagaag ttgatgaaga
     2281 gaaattacga gagaactttg catattttct caaagcaatt atcccggcat gtgaagaagt
     2341 tgggattaaa atggcagttc acccagatga tccaccatat tccattttcg gattacctcg
     2401 tattgttaaa aatcgtgacg atttagattg gttgtgtaat gtagtggatt ctccttctaa
     2461 tggtattaca ttatgtactg gaagtattgc agaacgacca gataatgatg tctatgaaat
     2521 tctatctgaa ttttgtcaac gggatcggat tccttttgca catgttcgta atatcaaata
     2581 tttaagtgac aatggactcg atttttacga agcgccgcat aaatcagaat atggttctct
     2641 agatatgtat aagatattaa aaacaatgta cgataatgga tttaatggct atattcgtcc
     2701 agatcatggt cggatgattt ggggagaaac cggtagacct ggttatggat tatatgaccg
     2761 agctctaggt gcagtttatt taaatggtat ttgggaagcg cttgaaaagg gggcgagtaa
     2821 ataattatat aagggagttt atttagagga aaaattttct cttctaa
//
LOCUS       DAYYGO010000188         2863 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000188 DAYYGO010000000
VERSION     DAYYGO010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.195"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            111..1253
                     /locus_tag="ACHA5T_002750"
     CDS             111..1253
                     /locus_tag="ACHA5T_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM8888267.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYKNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            1246..1452
                     /locus_tag="ACHA5T_002751"
     CDS             1246..1452
                     /locus_tag="ACHA5T_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFM8888268.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            1531..2724
                     /locus_tag="ACHA5T_002752"
     CDS             1531..2724
                     /locus_tag="ACHA5T_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8888269.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKTSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNEQRANVKKST
                     MKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKEKGFSYNTINNHKRSLKASFY
                     IAIQDDCVRKNPFDFKLSEVLENDTKEKVALTEEQEQALLSFIKTDNVYHKYYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKNGTRQVPLSR
                     ETIQAFQRVMKKRPKAEPFVIDGRSNFLFVNHKGKPKVAIDYNALFVRMVKKYNKQHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
BASE COUNT          995 a          532 c          638 g          698 t
ORIGIN      
        1 ccccaaagct gtaaggagta atgaggagtt ttaagaagat agtaatttca cattcttttg
       61 aatagtggtt tacaggtatt tcaaggctat tgtgggattg gaggaataca atgaacaatt
      121 tagaatttgc acttgaaatg aagaataaac gtcttgcaag cggtctttca caaggagaac
      181 ttgcaagtct tacccatgta tcaagatata gtatcaaccg ctttgagaac ggaaaagcca
      241 acgccagcaa ggaaacacaa aatattatcc tgcgttgtct gaattactat atctgcgata
      301 agccttttta tctgctcgtt gattatctgt ctgtccgttt tccaacgact gacgcattgg
      361 aaattatccg aaaggtgctt ggcatgaaag cagattattt tatccattat gactatggat
      421 attatggcta taaagagcat tacgcctacg gagaaatcaa ggttatggct tctgatgatg
      481 aacacatggg cgtatttttg gaattaaaag gggcaggctc acgcaatatg gaatatgttt
      541 tacaggcaca gaacagggat tggtattcat tcttgaaccg ctgtcttgac tgtggcggtg
      601 ttatccgacg ttttgacttg gcaatcaacg atatgtgcgg tctgctggat attcccgttt
      661 tatctgaaaa atacaaaaat ggcggtgcgg attgtcgctg taaaaactat gaaaatgtgc
      721 aaggcggtaa actaagcgga aaaaaccgca atttagcaag caccctttat atcggctcaa
      781 aagcaagcac aaaatatttc tgcctgtatg aaaaacaaaa ggaacaggca actaaaaaga
      841 aacatacgga tattatcaac cgttttgaaa ttcgtctgcg tgacaaaaag gcagtacagg
      901 cagttgagga actgttattg acgtataacc ctcatgggct ggtgttttac cttattactg
      961 attttgtgca atttcccgac tatccgctgt gggagatatt catttcccat gatagtttac
     1021 cttttgaaat gaaccccgta cctgtcaata tggaacgcac tctgcaatgg ctggaaagac
     1081 aggtcatgcc gtcaatcgtg atgatagagg aaatcgacag gctgacaggc tcaaactaca
     1141 tgaaaatgat tgatgaatgt acccaccttt ctgaaaaaca ggaaatgctt gtggaacaaa
     1201 tgtgtacgga catagcagac gtaattgaaa gtgagggggt gttttatgag taatgcacag
     1261 gatattcccg tatgggaaaa atacaccctt accattgaag aagcgtcaaa gtatttccgt
     1321 atcggagaaa acaagttaag acgcttggca gaggaaaaca aggacgctgg ctggctcatt
     1381 atgaatggca accgcataca gattaaacga cgacagtttg aacaggttat tgataaattg
     1441 gacgcaatct aatgcaaatg agccttgtat gtgttatgat gaacacaagt catatcaagg
     1501 ctctttccaa caaggaaagg agcagacacc atgaaagaaa aaagacggga taacaaagga
     1561 cgtatcctgc atactggaga gagccaacga acagacggaa aatacttgta taaatatgta
     1621 gacgcatttg gaaatacaaa atatgtgtat gcttggagat tgacacccac agacccgaca
     1681 ccaaagggaa aacgggaaaa aacctcactt cgtgaactgg aacagcagat aagacgggat
     1741 attgaggacg gtatcgacag cacaggcaag aaaatgacgc tttgccaact ctacgccaaa
     1801 cagaacgaac agagggcaaa cgtgaagaaa agcacaatga aacaacggga acagctcatg
     1861 cggttattga aagaggacaa gttgggtgct aggagcattg atacgataaa accctctgac
     1921 gctaaggaat gggcgttacg catgaaagag aaaggctttt cctacaatac tattaacaac
     1981 cataaacgct cgttaaaagc gtcattctat atcgccatac aagacgattg tgtaaggaag
     2041 aacccttttg atttcaagtt aagtgaagtc ctagaaaatg ataccaaaga gaaagtggca
     2101 ttgacagagg aacaagaaca agccctactg tcatttatta agacggacaa cgtgtatcat
     2161 aagtattatg atgatgtgct gatactgtta aagactggac ttcgtatctc ggaactgtgc
     2221 ggactgacag tagccgatat tgatttcaag aatgaagttg tgattatcga ccaccagtta
     2281 ttaaagagca aggaacaggg ctattatatt gaaacgccta aaacaaagaa cggaacaagg
     2341 caagtgccat taagcagaga aacaatacag gcatttcaac gggttatgaa aaaacgccca
     2401 aaggcagaac catttgtgat agacggacgg agcaattttc tgtttgtcaa tcataaaggc
     2461 aagcccaaag ttgcgattga ttacaatgcc ttatttgtcc gtatggtaaa gaaatacaac
     2521 aagcaacaca aggacaatcc cttgccacat atcacaccgc atacgctacg ccatacgttc
     2581 tgcacaaggc tggcaagcaa gaacatgaac ccgaaagatt tacagtatat catgggacat
     2641 tcaaacatca gtatcacaat gaactggtat gctcatgcgt ccatagatac cgcaaaatca
     2701 gaggttcagc gtctaatcgc atagaagtat ttaccacgat tttaaccacg tttgatagcg
     2761 aaaatataaa aagatagacc tagatatgtg aggtttacca caaaagcaaa atgcccgtag
     2821 agccgataaa ataaggcttt gcggacattt gagaagatat aaa
//
LOCUS       DAYYGO010000189         2831 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000189 DAYYGO010000000
VERSION     DAYYGO010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.196"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            275..2170
                     /gene="asnB"
                     /locus_tag="ACHA5T_002753"
     CDS             275..2170
                     /gene="asnB"
                     /locus_tag="ACHA5T_002753"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFM8888270.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(2365..2811)
                     /locus_tag="ACHA5T_002754"
     CDS             complement(2365..2811)
                     /locus_tag="ACHA5T_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFM8888271.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
BASE COUNT          920 a          485 c          535 g          891 t
ORIGIN      
        1 gacacgatct ttaggtcgtg tctttttttg ctggtttttt tggaaatagc atccactaat
       61 atttttttaa cacgaagaga ataaggtagc ctcaaaaaag aaaacatggt aaattagata
      121 ttgggacttt attttctgta aaaacagaaa ctttcatctt ttgttttcac gtgacgaaat
      181 tgtaagagag aaaatattcg tccgctattt attctatgat aagatgcata tgaatgaaat
      241 aaacagtaat tttatattag gatgtgaaag ttaaatgtgc ggtattgtag gttttgttaa
      301 cgacaaggac aacaaaaaaa caattattaa tacaatgatg gatcgaatcg ttcaccgtgg
      361 acctaatagt tcaggagaat atatcgataa acatgttgct ttaggattca gaagattaag
      421 tattattgac ttagagggcg gtactcagcc tatttacaat gaagatcgga caaagatcat
      481 tatttttaat ggcgaaatct ataattacca gcctttgaga gaagaattga ttgcagctgg
      541 ccacgttttt caaacacatg cagatacaga agtccttttg catggttacg aagaatgggg
      601 aacagaatta ttgcaaaaga tccgcggaat gtttgctttt gctatttggg ataatgaaaa
      661 gaatgagtta ttcggtgcaa gagaccactt tgggatcaaa ccatattatt atgcagaaat
      721 gaacggaaca tttatgtttg gttcagaaat caaaagtttt ctgcctcatc ctgattttaa
      781 caaagaattg aacaaagaag cgttgaaacc ttatatgact ttccaatatt ctccattaaa
      841 cggcgaaaca ttcttcaaag atgtctatcg tttgcctgaa ggccattatt atacgtataa
      901 agatggaaaa atagacattc agcaatattg ggatgcagat tttgaaacaa aagaaacgca
      961 ttcccgacaa gaatggatcg aaaaaatcga cgaaaccgtt caagcttcta ttgaagcaca
     1021 tacggtcagt gatgtagaag ttggttcttt cttatccagc ggggttgatt ccagttatgt
     1081 aacctctgta ttgaaaccag atcattcttt ctcaatcggg tttgatgata aaacctataa
     1141 tgaagcgatt gaagccagaa aactaaccga actattggac ttagataata cagcagcagt
     1201 gatcgacgga gacatgtcgt tcaaagcatt ccctttgatc cagtatcact tagatgaacc
     1261 tgattcaaat ccttcttgcg tacctttgta ctttttagca aatcttgctt cacaaagtgt
     1321 gcgggtagtc caatctggag aaggggcgga tgaactgttt gcgggatacc aaacgtatgg
     1381 attccatacg aattcaaaat tcatccgtgt gattgctcaa ggattgaaaa aattaccaaa
     1441 aggaacacgc tataatttag gacgtaaaat cggaaaaatg aaaaacttcc atggacggat
     1501 ccatttatat gaatcacttg ctcctgcaaa agaatatttt atcggtcatg ctcgagtgtt
     1561 cgaagaatca gaagcatcag aagtgttaac accaaaatat caaacagcac catctgtgga
     1621 tgaaatcatg actgttcatt acgagaaaac agaaggaatc aaagatgaag tcaataagat
     1681 gcaatatgtc gatcttcatc aatggatgcc aaaagatatc ttgttaaagg cagataaact
     1741 ttctatggca agttctttgg aagtccgagt acctttattg gatatcgaag tcatgaaatt
     1801 ggctcaacaa ataccaagta agtttttatt gaatcaaaac aacacaaaag atattttcag
     1861 acaagctgcg aacaaacatc ttccggaaga atggtcgaat cgtgtgaaat taggattccc
     1921 tgtgccaatc aaagcttggt tgaaagaaga acacggctac gaacaagtca aagcattatt
     1981 cgaagcagat tttgcaaaag aattttttga tcaagaaaaa atcatgaagt tgctagatga
     2041 ccatcacgaa ggacgtaaag aagagcaaag aaaaatctgg acaatcttca gtttcttgac
     2101 atggtataaa gtctactttg tagatgaaac gattccgcaa gcagaagcaa tcgactatgt
     2161 gactgtttga tgaaaatcaa atatcaataa aaaagaggtt ttctcctacc gtttaacact
     2221 gagtgataag aaacaatttc aaaaaacagc tcttcccgtt ttatagatat tatgacttat
     2281 ggttcggagt tggcagagga acaccattgt ttcgaacata agggggttgt gacacgattt
     2341 tcgtcacaac ccctttacat atgttcaata atcgatttct ttttcttttg tcccatcttt
     2401 tttataaatg aacaaagttg attctttgtt ttgtgctact tgctttcctt tttcgattgc
     2461 ttcttttttt gtatcaaaat ggttgcttgc cttttttgcc cctttagatt tcacgatcca
     2521 ttgttcttcc tcatattcta cgatcacatc tgaatcaagc agttttccag cacgaggatt
     2581 cgtatcgtgt tcatccgtct tgcttggatt tttttctttt tcgaaagttc gtttttctga
     2641 ttcactagca tcttcatacc atttttttgc ttgatcaatg gcaattggga tcgcacggtt
     2701 atcagggtac ccttcatcta ataatgcatt tgcgatgtcg attgcttttt ttcttgtcaa
     2761 tttatctaag tttttcattg atgcaggaaa gtccttcatg ttccatggca tattagtatc
     2821 cccctttatt a
//
LOCUS       DAYYGO010000190         2786 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000190 DAYYGO010000000
VERSION     DAYYGO010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.197"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            103..1110
                     /locus_tag="ACHA5T_002755"
     CDS             103..1110
                     /locus_tag="ACHA5T_002755"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050377 - UDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase family
                     protein"
                     /protein_id="HFM8888272.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1122..2126
                     /gene="neuB"
                     /locus_tag="ACHA5T_002756"
     CDS             1122..2126
                     /gene="neuB"
                     /locus_tag="ACHA5T_002756"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HFM8888273.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2123..>2786
                     /gene="neuC"
                     /locus_tag="ACHA5T_002757"
     CDS             2123..>2786
                     /gene="neuC"
                     /locus_tag="ACHA5T_002757"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFM8888274.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQ"
BASE COUNT         1033 a          352 c          543 g          858 t
ORIGIN      
        1 cgttacgttt tgacatcatt attgataatt agtttagtta tatatttttt aaagattaag
       61 aatcgaatta tagatgacta taattcatag gaggagatat aagtgtttaa aggaaaaaca
      121 atattagtaa ctggcggaac tggttccatt ggaagtgaaa ttgttcgtca gcttttaaaa
      181 tatgaaccaa aagctatcag aatttatagt agaggtgaag aaaaacaatt ttatatgcaa
      241 caagagctct caaatcacac taatttgaga tttttaattg gtgatatcag agactctcaa
      301 aggattgatt atgcatgtcg tgatgtggat attatttttc atgctgccgc aatgaaacac
      361 gttccagcaa gtgaatataa tcctatggaa gctgtaaaaa caaatgtgat gggaactcaa
      421 aatgtaatag atgcagcgat caaaaataat gtaaaacatt ttgtaggtat cagtacagat
      481 aaagtagtga gtcctactaa caccatgggt gcgacaaagt tattgagtga aaaattaata
      541 atagctgcca atcagtataa aggaaaaaca gaaacaatat tttcttgtgt aaggtttgga
      601 aatgtcatgg ggtcaagtgg atctgttatt cctcttttta gtaaacaaat aaaaaacaat
      661 aaagatatta ctttgacaga tgttgatatg acacgattta tgatgtcaat accggatgct
      721 gcaagattag ttctgtctgc tgctatgaat tcaaaatttg gagaaacata tgtcttaaaa
      781 atgccggcaa tcaatatttt aactttagct caatcattaa taaaaatata taatgataca
      841 catgatgaaa aatatagagg tgaaataaaa gttgtaggta tcaggccagg agaaaaatta
      901 tatgaagagc taatgactac agacgaaatt gaacgctcat atgaaaatga taaaatgtat
      961 atcattccac cacactatca tgctactgac gaactgtaca aagattatga aaaaattaaa
     1021 atcactgcat actcttcaga tgcaacagaa ttaatgagtg gacaggaatt tattagaatc
     1081 gcaaaagaga ataatgtcgt gacttggtaa ggagttttat gatgaaatca gaagtatatg
     1141 taattgcaga aattggtgta aaccataatg gaagtctaga tttagcttta gaaagtattg
     1201 ataaagctaa agaatgtgga gctaacgcgg ttaaatttca aacatttaaa acgaataagc
     1261 tagtttccaa aaaggctcca atggcaaaat atcagaagga taatcttaaa gaggacagtt
     1321 ctcaatttga aatgctaaaa aaattagaat tatctcaatc tgattttatt agaataaaag
     1381 attattgtat acaaaataaa attgattttt tgtctactcc ttttgatgaa gaaagtgcag
     1441 actttctaaa acaaattggt gtacaaggat ttaaaattgg ctcaggagat ttaacaaatt
     1501 taccttttct tagaaaaata gatatgtatg ggttgccagt tttattatct acaggtatgg
     1561 ctgatattca tgaggtcagt gaggctgtag attgtttcat agatagtcca gttactattt
     1621 tacattgtac ttcaaattat ccggctagtt ctgaagatat taatttattg gcgattaaca
     1681 cattaaaaga aatgttcggg atacccgttg ggtactccga tcattcattg ggatatgatg
     1741 ttggtatttg tgctgttgcg atgggtgcta aggtaattga gaaacatttt accttggata
     1801 aggaactacc tggtccagat cataaagctt ctttagatcc tcaggaattt gcagattttg
     1861 taaagcatat tagaaacact gaaatatttt taggtgatgg tgttaagcga gcaatgcctt
     1921 ctgaacttag tacaaaagaa gtatcaagaa aaagcctagt ggtcactgag aatttaaatg
     1981 tgggagatat tcttacagag aaatctctta caataaaacg tcctggaaat ggaatagctc
     2041 ctaagttttt atatcaatat attggcaaaa aagttaaaag accaataagt gcagatagtg
     2101 ttctgcttga ggaagatgtt gaatgaaaaa aatacttata gtaagtggaa cacgagctga
     2161 ttatggaata tataaacctg ttgctgagaa tttactacaa gataattggg atgtatcatt
     2221 tgttgtttct ggaatgcatt tatcccataa atatggattt actaaagaat tgatcttagc
     2281 tgacggattt agaattagtg gagaagttca aagtttgttt ttagagaaca ataagggaaa
     2341 catggctcgg tctgtggcat tagagataaa tggtttcaca aatatatttg aacaaactag
     2401 ccctgatttt gtattattac taggggatag aggagaaatg ttggcagctg cgatagcttg
     2461 tttgtatctt ggaatcaaaa cagcacatat tcacggaggc gaagtgtctg gtactgtaga
     2521 tgaatcaata agacatgcca taacgaaaat agcatctgtt cattttgctg ctacggaaaa
     2581 aagcaaagag cgtttaataa aaatggggga agattcttgg agagtttttc ccgtcggagc
     2641 tcctagaata gatactattt taaataatag attacctagt tttagagata taaaagaaaa
     2701 atatcatcta aaatttgaaa agaaaaaata cgttttgcaa gtatttcatc cagttgtaac
     2761 cgaactagaa aatatagaaa aacaaa
//
LOCUS       DAYYGO010000191         2763 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000191 DAYYGO010000000
VERSION     DAYYGO010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.199"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            107..553
                     /locus_tag="ACHA5T_002758"
     CDS             107..553
                     /locus_tag="ACHA5T_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM8888275.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            826..2178
                     /locus_tag="ACHA5T_002759"
     CDS             826..2178
                     /locus_tag="ACHA5T_002759"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFM8888276.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            2349..2636
                     /locus_tag="ACHA5T_002760"
     CDS             2349..2636
                     /locus_tag="ACHA5T_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM8888277.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT          974 a          451 c          545 g          793 t
ORIGIN      
        1 ttttttcatt aaaatacaat aaaattgaat tttcttttca tgttaagtgg attttaaaat
       61 gtaaaaatag tatgataaga cgagaataac aacgtggggt gtttacatgg aaaataagtt
      121 agtcgaagag gttatcgaaa agttaaaaaa agagaacatt cgaatcacac ctcaacggta
      181 cgcggtttta gaatatttga ttgaacatca ttcccatcca acggctgatg agatttataa
      241 agcacttgaa catcggtttc caaatatgag tgttgcgact gtttacaata accttcgctt
      301 atttacttcg attggatttg ttcaagaaat gaagtacggc gatgcttcta gccgatttga
      361 tttcagttca aagcgtcatt atcatgtaat ttgtgaaaac tgcggtaaga ttgttgattt
      421 tcattaccca gggcttgaag atatagaaat ggctgcaagc agactgactg attttgagat
      481 taatgaacat cggttggaat tgtatggact ctgtccggat tgtaaagagt cagcaaaaaa
      541 ggcgcaaatg taaaaacagt ttctacaagt gttataccta aaaaaagcta gctgtgtaaa
      601 aaaaacgcta gcttttttat ttttttgtct tttattcaca atcttagata taaaagtgaa
      661 tattattaaa ataagaatct tgtagatatt gggttcttat gcttgaaaag gaatgaaact
      721 acaaacaaac ctttgtgaat tcacaagcat taaactgttg tatccgtttt atttctgtta
      781 tagtagtttt gtcgaaggac aaaatgttaa tcggagggac cataaatgaa agtagtagta
      841 attggctgta ctcatgcagg tactgcagct gtaaaaagta ttttaaaaaa tcatccagaa
      901 gcagaagtga cagtatatga aagaaatgac aatatttctt tcttatcttg cggtatcgca
      961 ctttatgtcg gcggagtagt aaaggaccca gcaggcttgt tctactcaaa tcctgaagaa
     1021 ttatcttcat taggagcaac agttaaaatg gaacacgatg taacagacgt cgatacagaa
     1081 aataaaacag taacggcaaa agatctgaaa acaggagaaa cagaaacggt ttcttatgat
     1141 aaattagtga tgacaactgg ttcatggcct attatcccac cgattagtgg aatcgatgcg
     1201 aaaaatgtac tcctatgcaa aaactacaat caagcaaatg aaattattgc acaagcgaaa
     1261 gaagcaaaac gtgtagtaat tgttggtggt ggatatatcg gtattgaatt agtagaagct
     1321 tttgtcgaat ctggaaaaca agtgacattg attgatggat tagatcgcat cttgaataaa
     1381 tatctagata aaccattcac agatatctta gaaaaagaat tgacagaccg cggtgttact
     1441 cttgcgctag gcgaaaatgt tactgaattt atcacagatg aagaaggaaa agtgaaacaa
     1501 gtagctacac caacagatac atttgataca gatatggtca ttttatgtgt gggcttccgt
     1561 ccaaatacaa aactgatcga aggaaaagta gatacactgc caaacggggc aatcaaagtg
     1621 aatgaataca tgcagacaag taatccagac atttttgcag ctggagactc tgcagtagtc
     1681 aactacaatc caagtggcac gcaaaactat attcctttgg caacaaatgc cgttcgtcaa
     1741 ggtttgcttg tgggaaataa cttgacagat cataaaatgg cgtatcgtgg tactcaagga
     1801 acttcaggac tttacctatt tggctggacg atcggttcga caggtgtgac caaagaaagc
     1861 gcaactttga acgatctaga agtccaagcg actgtatttg aagataatta tcgtccagag
     1921 ttcatgccaa ctactgaaaa agtaatgatg gagctagtct atgaaaaagg aacgaatcgt
     1981 atcgtaggtg ctcagttcat gtccaaatac gacattacac aatcagcaaa taccatgtct
     2041 ttagctgttc aaaataaaat gactgtagaa gatctcgcac tttcagattt cttcttccaa
     2101 ccacattttg accgcccatg gaattacttg aacctacttg cacaagcagc gctaggcgaa
     2161 atcgaaactg cacaataaag aaaaagggtt gtgacacaat tttgtcacga ccctctgtct
     2221 ttttttgtga aaagtacaat tagttgtctt tcttcgaatt ttttgtcaca ataagcagca
     2281 aagataaagc tgttacataa aagaacaagc agctttattt gattttgaaa agaaaggtga
     2341 gaaagaagat gaataaagaa tattttgtcc aacaagatgc atttttagaa gattataaaa
     2401 gtgcttcgaa gaaattaacg atcaatattg atgagttggc agatcaagta ctctctcagt
     2461 tgaatgaaac gcaagaggaa tattacacga tgccagcaaa tgacgccaaa gataatgttt
     2521 ctcatcgttt tcctttcagt agacgcatct actcagatga aaatgacggc acattgaata
     2581 cagagtacac atatgaaggt cagccatatg tgctagaaac tgatgatgat ctttaaagac
     2641 agtttttgaa aaggccgctc atccttaagt gatgacaaaa aatcatcaca attaccgatg
     2701 actgtctctt tactatcttt taatcagaaa aaggggttat gacaagcttt gtcaacccct
     2761 ttt
//
LOCUS       DAYYGO010000192         2723 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000192 DAYYGO010000000
VERSION     DAYYGO010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.200"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..939
                     /locus_tag="ACHA5T_002761"
     CDS             <1..939
                     /locus_tag="ACHA5T_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM8888278.1"
                     /translation="INNEFNLGPNEGSSQVANNQYILLHETANETATGRNEAQYMKRS
                     WTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKATFEKNYKA
                     YVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKL
                     AYDLAHGFTDENPTTSDDKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIENANLHVAG
                     WHIANYKYEYIFIMDYNTGKELARVKADGIYRPDVNQAYNTLGNVGYHVSFNMRNFPN
                     KKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPQR"
     gene            1243..1650
                     /locus_tag="ACHA5T_002762"
     CDS             1243..1650
                     /locus_tag="ACHA5T_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888279.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            1664..2065
                     /locus_tag="ACHA5T_002763"
     CDS             1664..2065
                     /locus_tag="ACHA5T_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888280.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            2067..2438
                     /locus_tag="ACHA5T_002764"
     CDS             2067..2438
                     /locus_tag="ACHA5T_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888281.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(2474..>2723)
                     /locus_tag="ACHA5T_002765"
     CDS             complement(2474..>2723)
                     /locus_tag="ACHA5T_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM8888282.1"
                     /translation="FVKDFYKVWDSEVFFLAIEDSLLINLYYSNKNYFKIPAAKTRMK
                     KDVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT          992 a          414 c          497 g          820 t
ORIGIN      
        1 atcaacaatg aatttaattt gggcccaaac gaaggtagct ctcaagtagc aaataatcag
       61 tacattttac tgcatgagac agctaacgaa acagcaacag ggcgcaatga agcgcagtat
      121 atgaaacgtt catggactag tgcttacact gcttatattg tgggagacgg cggaattgtt
      181 taccaagtcg gacaacctgg ttatgtacag tacggtgctg gttcgtatgc taatgctaac
      241 agtcccgtgc agattgagtt acaacacaca catgataaag caacgtttga gaaaaactac
      301 aaggcatacg ttgaattagc tagagattca gcaatgaaat atggtattcc acttacacta
      361 gacacaccgt ataatcaacc agggatcaaa tcgcatttat gggtaacaca aaatatttgg
      421 ggcgatcata cagatcctta cggttatctt tctgaaatgg gcgtaagtaa agaaaaatta
      481 gcctatgatt tggctcatgg atttaccgat gaaaatccga caacttcaga tgataaacca
      541 gtcattgatc caactcgagc aggtgctgca aatcctaccc tgacagatgg aacaaattac
      601 gcccacattg atcagttcgg agaaatcgaa aacgcaaact tacatgtggc tggttggcac
      661 atcgctaact ataaatacga gtatatcttc attatggatt acaatactgg gaaagaatta
      721 gctcgagtaa aagctgatgg aatatataga ccagatgtaa atcaagctta taatacttta
      781 ggaaatgttg gctatcatgt atcttttaac atgcgcaact tccctaataa gaaagtctat
      841 gtaatgatgc gggcaacgaa tgatccagaa gggaacacta aaggcggagc acaagatttt
      901 catgataaac gctggtattt aaatattccg caacgataaa aatagcccct catttttgag
      961 gggaagtaca tatagttgtt gatatagttg ctaatattgt tagctaattt gttagtaaaa
     1021 ctgtatacga atttagatag taaaaaaaca gtttgttcct tatatagagg cacttttact
     1081 actgattaaa tgtcagatat gcttacaaat atacttataa atatgcttac aaaattagct
     1141 aatatttccg ttgacaaagc tttgttacta ttgttaaact aaattgacta gcaaaagtga
     1201 aacataaatt tattaaataa atgtaaaaat taggagaaat caatgtctta tagagtacaa
     1261 ttaataattt cagaagatgt agaaagtcag caatttggca ctaatgttat taataaagta
     1321 attaatccag ctctttctat taatgcgcct ttaattccta ctgctttatc attttctgta
     1381 acagctgtgg tgtctgagat agaggataca gaaaagataa aaatagtaga aattgaagtt
     1441 ttaaacaaga atgaaaaaca aattttttct acgggtgaag tatcggtaag cttgcctcct
     1501 caagttaacg atataaactt taatataaac gccagaaatg tcttggttga agaagcagga
     1561 gagcattatg ctgttgttaa atttaatgga actgagattg gaaggcaaat ctttgatatc
     1621 aaggtcaaca aaccagtgga aaaaaattaa ggagacgata cggatgcttg atatagtacc
     1681 atcaaataca catcggagtg gtagcttaat aaattttgct gcgatttttg cttgcgcatc
     1741 ggtaattcta gcatctccag ttgttgtgga tgcaagttct actccgaaaa ctaaaaatga
     1801 taatcaactt gtaattacta caaaaaatga gattaacaca atttcaagta atgatggcaa
     1861 aatttttgat gtgataaata cggttttaaa aagaaaagct gaaatcaata taaaatatga
     1921 tgaagattta aacttgtatt tctttgttat caaaacaaca tctgaattgt tttcctcaga
     1981 ttacgatgtt ttggatactc tcgataatgt attatctgac tataaatata tggggaaatc
     2041 tgtagtagca actttggggg aataaaatgt ttgactttaa tgaatattta tctatttcca
     2101 aacaaattga atcagatgat aaatataatt ctaaagaatc ttatagaaga actgctgttt
     2161 caagagcata ctacagtgct tttaaaaaat ctgatgagta tttgaaagaa aattatgata
     2221 ttatttataa tggaagtagc ggaaaaggaa gccaccaaac cgtttggaat ttgttttcaa
     2281 cagttaagga attgaatacc ttaggaatac ataatagtgg gtatagaatg ttagagaaaa
     2341 gaaaatgtgc tgactatatt cctaatgaaa ccattacgaa aacagatatg gcgctgatga
     2401 atcgagaagc agaaaaaata ataaacaaac tcacctaatg aatttcaagc gagtttgttt
     2461 gttatttttt tatttaatct ttgtatcttt ctggatcaac gaaagtatac tttatatagt
     2521 cataacgccg atgatcgcta cgtgcgtccg gcacgtcagt cacgatatca aacaaaaagt
     2581 atacatcctt cttcattcta gttttcgcag caggaatttt gaaatagttc ttattagaat
     2641 agtagagatt gattaataag ctatcttcga ttgctaaaaa gaaaacttct gaatcccaca
     2701 ccttataaaa atctttgaca aat
//
LOCUS       DAYYGO010000193         2698 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000193 DAYYGO010000000
VERSION     DAYYGO010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.201"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(241..606)
                     /locus_tag="ACHA5T_002766"
     CDS             complement(241..606)
                     /locus_tag="ACHA5T_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888283.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            814..1296
                     /locus_tag="ACHA5T_002767"
     CDS             814..1296
                     /locus_tag="ACHA5T_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888284.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSTFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1493..2383
                     /locus_tag="ACHA5T_002768"
     CDS             1493..2383
                     /locus_tag="ACHA5T_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFM8888285.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT          918 a          438 c          528 g          814 t
ORIGIN      
        1 ttaattagtt tactttcttc aacactttat tctttgaata ttatagatgt ttccacctca
       61 aattcaatat ctattattgg aatcattatt gctgcaatta ttacacaact gtttttagtg
      121 tttggagaga agaaaagaag taaaaaatag aaaaactagt gatagttttg gtgatataag
      181 aaaaacatat atgtataaaa agaagaaagt agcttctctt tgtacatata tggtttgttt
      241 ttatggatgc cgagcttttt tataagataa ggctaccgcc cataacgacc caaggatttg
      301 tggaactaag taaatacctg caagaagcaa gatcagctgg gtcggtttta aaggatgata
      361 gaagtaacgc cattcagtaa aattggcgca aaataaatag gaagtgacag tagatataat
      421 attggcaatg aataaagata taatacgcct gttcctttga aaataatatc cagcaaaaag
      481 agataaaaat atcaatgtga tgaacccgct tacggaatga taacgaaaat caagcagtaa
      541 ggcgaaatgc acataaggaa caaaagataa aagcaacgga aggtttttcc agcaagtttt
      601 tatcatcttt catcaccacc taacaagtgt tattattttg ttagttttat tgtaaattga
      661 atcctgcaat aatcaattac tttttcatta tttctagatt aaatgaataa actctcatgc
      721 cattatctct aaaaaaaaag tttttccccc taattttttt agatagaaaa ttgtatagta
      781 tgatgagaga aaaagtagtt tgggggaacg ttaatgagaa gaaaagcagt aatcgatcgg
      841 ctttatttat ttttttggtt ttttcctatc gttttgctac cttttacatg ggtaaatgct
      901 gttgtacaag gtgcggagat attaggcctg atccaaggaa gcggatttgt ggtttctagg
      961 aattttccag ctttgattgt tttctattta gtgataagtg taggtctctt attaccaaaa
     1021 tctctgatca atcactacaa ttggcagctg ctttactttt cttgcttatt agtatttaca
     1081 tctacgtttt ctttgctgcc tcgcttgatt atcggcccta atggtttgag cgagagtgga
     1141 ttgaaggatt tagcttattc ctccatgtat ttcttgacga tcaaaccatg gttctattta
     1201 gtaattgcct catttattct tgcaacattt ttttacttct ggacagaaat caggataaaa
     1261 agtgaacgat ctgtatctat aaaaaataac tattgatggt ggatcatcag taaaaaaaca
     1321 tatgagctgc gtaaaatgca tgtttctcat atgctttttt tattattatg taaagaatgc
     1381 gaaaaacgtt tacatgattt ttacataact taactatatt tttacagcaa ccatacgagg
     1441 gatttacagt cgactgataa actttagttg taaataagaa aggagcaata aaatgaaaaa
     1501 agtattgttt actctaggta tgatgggaat tcttttagcc ggatgcggct caggatcagg
     1561 aaatacagat ggctctgcca cacaaaataa ttcaagtgac tcaaatgaac aagtaaaaat
     1621 cgttgcggta ggttcgacag cattacagcc gttagtcgat gcagcacaag aaagctttgt
     1681 acaagaaaat ccaaattatc aaatctctgt acaaggagga ggaagcggaa ctggtttgag
     1741 ccaagtcgaa gctggagcag ttactatcgg aaactctgat gtatttgcag aagaaagaga
     1801 cggagttgat gcttcaaaat tagtggatca taaagtagca gtggtcggga tggcaccaat
     1861 cgtcaataaa gatacagatg tcaaagatat caccaaacag gaattgattg atatctttac
     1921 aggaaaaatc actaactgga aggaagttgg cgggaaagat caaaaaatca acgtcgtgaa
     1981 ccgtgcaaac ggcagcggta cccgcgcgac attcgaaaaa tggggactgg atggtgctac
     2041 ccctgtgcag tcccaagaac aagattcatc aggaacagtt cggcagctgg taagtcaaac
     2101 accaggagca atcagctatt tagctttctc atatctcgat gattccacac aagcactaag
     2161 tatcgacggt gtagaaccaa aagaagagaa tgtagcagat aacagctggg gaatctggtc
     2221 atatgaacat atgtacacga acggaaaacc ttcccctgaa gtccaaaaat tcttagacta
     2281 catgatgaca gaagaaatcc aagaaggacc tgtcaaagaa ttgggctatt taccaatcac
     2341 aatgatggaa gtagaacgtg atcacgaagg aaatatcaaa taactagcag ataaacatgc
     2401 cacggtagtc gttactatta aggaaaaatc aactatgttt ccccacatat acatttttcc
     2461 tttaattgtg aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca
     2521 gctgattgaa caccgtttat tcgaaaaaaa gagctacgac atagaactgt cgtaactttt
     2581 tttggctctt cgtcaaatcg tgttggtgaa gacaaatttg aataaaactt agaccgatat
     2641 gcggcgcgtt ctgtgcgccg catatttttt tagttttcaa agatcagacc ttgttgta
//
LOCUS       DAYYGO010000194         2689 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000194 DAYYGO010000000
VERSION     DAYYGO010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.202"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(34..1128)
                     /locus_tag="ACHA5T_002769"
     CDS             complement(34..1128)
                     /locus_tag="ACHA5T_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFM8888286.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1317..2078
                     /locus_tag="ACHA5T_002770"
     CDS             1317..2078
                     /locus_tag="ACHA5T_002770"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8888287.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2193..2633)
                     /gene="fabZ"
                     /locus_tag="ACHA5T_002771"
     CDS             complement(2193..2633)
                     /gene="fabZ"
                     /locus_tag="ACHA5T_002771"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFM8888288.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
BASE COUNT          839 a          541 c          461 g          848 t
ORIGIN      
        1 ttctagcgga ttatttctgc aataactaat tatctatttt ttattttcaa ttaatttaaa
       61 gcttgaccat ggtttcaaaa agtcaaggag gaataattcg tcatcggaga ttcttcctac
      121 aacattgaca cgtccgtcgt ttttcatatc cttcaaggcg atttgtgttt ctcctttgta
      181 ttgaccgtac ccttcattgt caatcagtac atccccatga tgcatatctc gcgtattatg
      241 aggcgggaaa tctttatctt tgtagtatac acgagtcatc gttgaacgaa ggatatattc
      301 tgagcgatcc cctcgataac tatgaaggtt atcaaacaag cagattcgtt cgttttctgt
      361 aataccttct tccgtatcta ctttcaatgt tggataatcc gcgttaaatg cttctgccat
      421 ttctttcaat tcttcttcac ttgcgtacgc atttccaata gaaatatcat caatcaatcc
      481 tgtaaggatt aaatgcttca cttgtgtcgc aatttcaaga tctcggtggt cttccagcgt
      541 gcacaagcca tcttgcgtag gccaagggcc aaactcagca gattgcgaat tgacgaatgc
      601 catcgtattc aaattatatt ttctgaattt ttctgaacaa aaaacaaagt gttcgtatcc
      661 taaaccagag tatcgatgag gataaaaatt atgagaacct aataaattat ccgtgtttgg
      721 cgaataactc atgatgttgt caacgtaatt cgtgcccgaa ctcatattga tctcgatttt
      781 gataccaaaa ggattgcgcg tcatttttgc ttcttctgct cctgtgaatc cgatatctaa
      841 gcggaccccg taggcaccca tcttatggaa aaaagataag tcatcgtaag aaatctccaa
      901 ctgttcaaat aacgctggat tgatatcaac cattacttcc atgcctaggc tattcgcgta
      961 atcaactact tctttaaatt ctgaaagaac tttttcttta tcatcattga tctgcagcaa
     1021 actggtaaaa acccgtttat acccatactt atgagctaaa tccaagtacg ctttgtcctt
     1081 ttcaaatgtt gaccgttctg gataaatcga gattcctaat tttcccatat taccatttcc
     1141 tcctgcatct tttcgttttt gtatccactt acattatagc aaacacaaag taaatggtct
     1201 acaccttttc tttttctttt aaaataaatg ataaattcca ataatttaag taggtatttt
     1261 ccctctattt tgttatcatt aaagataaca aaatagtaaa ttggaaagga acagttatga
     1321 agaaaaataa agttatttta agtattagtt tagcagcagc attgctgttt gctgctggtt
     1381 gttctgcaaa caacacgact tcttctagtt cgtctacaga aagttcaaaa agtacatcta
     1441 ctgcggaaag cacgactgcc agtagcgtcg atttgaacgc attggaatta ccacaattga
     1501 cgaaagatgt cgccgaaaat gaagatctgg tcgaaatggt gacgaataaa ggcacaatca
     1561 aaatcaaatt atttcccgaa cacgcaccaa aagcggttga aaactttatg acacatgcaa
     1621 aagacggtta ctacaacgga ctgactttcc atcgcgtcat caaagatttc atgatccaag
     1681 gtggcgatcc taaaggcgat ggcactggag gggaaagcat ttggggagaa ggatttgaaa
     1741 cagagatcaa caaccacctt tataatataa gaggtgcgtt atctatggct cgctcacaag
     1801 atcctaacag taacggcagc cagttcttta tcgttcaaaa tacagatgat atgcatgatg
     1861 gtcttttaaa agacgactat cctcaagcaa tcatcgacgc ttataaaaac ggcggttatc
     1921 cttcactaga tggccattat actgtcttcg gccaagtgat tgaaggaatg gatgtcgtgg
     1981 atgctattgc aaatatcgaa acagatagtt cagacaaacc aaaagaagaa gtaaaaatcg
     2041 ataaaatcaa tatactgcaa gaagcaaaac aaaaataaaa aaataacgtc tgaatcctgc
     2101 atagatgaca acgtatgcta ggattcaggc gtcttctttt tacataaaaa gcaagtaagc
     2161 ttccttttgt tttgctatat caggttttat agtcaagcat tttggttgac gatgaacgtg
     2221 aactcgcact cacagacttt tttatcatct acatatgctg cagataacgc tgtaccgact
     2281 gatcccttca ttttggtgat ttcaaaatga catttcagca catctcctgg aacaactttt
     2341 tgacggaatt tggccttatt gatcccaccg atataagctc tttttcctag aaactcttct
     2401 gattttagga taagtatcga acctgcttgt gccagtgttt ctagaatcaa cgcacccggc
     2461 atcactggat tgtcaggaaa gtgaccttgg aaaaaatctt cattgattgt tacatttttt
     2521 gtcgcaacga tcttttttcc tggttctaaa gtatctacat aatcaatata acaaatagga
     2581 taacgattag gaatcaattc cattacttca gtagcagata atacgtaaga cataattaaa
     2641 ctccttcttt caggcaattg ccattttcat tactttgact atcaaagta
//
LOCUS       DAYYGO010000195         2561 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000195 DAYYGO010000000
VERSION     DAYYGO010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.203"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1410)
                     /locus_tag="ACHA5T_002772"
     CDS             complement(<1..1410)
                     /locus_tag="ACHA5T_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8888289.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQ"
     gene            complement(1474..1833)
                     /locus_tag="ACHA5T_002773"
                     /pseudo
     CDS             complement(1474..1833)
                     /locus_tag="ACHA5T_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
     gene            complement(2011..2331)
                     /locus_tag="ACHA5T_002774"
                     /pseudo
     CDS             complement(2011..2331)
                     /locus_tag="ACHA5T_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT          793 a          483 c          348 g          937 t
ORIGIN      
        1 ttgataaatc caagaattca aaaagataga cgttatttga agataataat agtcaaattc
       61 tatttgaaaa tctctctcta tttccttaac atactgtcta aatcgaatct cttgataggt
      121 gggatatgtt aaagctgaat tattttggat atcaacattc ttttgtctaa aatatctatc
      181 atatttctca aaaagctgct gcttatcagt gtctttcttc cacgtttgat aaagatactc
      241 tataagttcg ttgatattaa aaacttcgtc tactttttcc aaatcatcca tttgaatata
      301 ttttaattca ttctgtcttt tcataacctt catgccgttg aaattcaccc attgtggtaa
      361 atgatacatc tgaatataaa gttcttccaa atcatctaag aaataaaagg cattggcaca
      421 ggtcaattga atgatatttt tcaaattgcc aacattttca ctaaagcgat agtttaataa
      481 aaaatctaat aattgttgag atatcagtat tttctttgct agtctttctt cttctgattt
      541 aaaaaatatc ttgattaact gttttctttc ataaagagaa cgactgtcta atgttggaag
      601 tgttagcttt acaggtattc ttctcaacaa agttgataat agtttatcct caggtggttc
      661 tgatgttgca aatataatcc agcaacttga tgtgtaccag ttttcattat cacccatccg
      721 atgaaatttc ctcgtatcca taaactgaaa aagtttctcc tgacattcat tgctcaatgc
      781 atgaatttca tctaaaaata agactccatt attagctaat tctgctaaac cctttcttcc
      841 atcttcggct cctgtatatg caccttttac atgcccaaaa aaattggaca agaaaagttc
      901 tggattattg gcatattcac tacaattaac tgaaatgaaa gatgccttct cttctatata
      961 attattggcc aacaaatcct tatacacata atttgctaaa tagctttttc cagttccagt
     1021 ctcgccatga agtaaaatgg gtaagccatt tggcggatag tagattgctg ctttaatttg
     1081 agcaattaca tttcgcagac ttccttgact tccgataacc ctttctattc caaccctaaa
     1141 agagctatca taccattttt ccgcttcctc tgaagagcta aaaatttgtt gatttggaac
     1201 tttctccggt aaagaaaaga agattactgg acgagtattg atttttccaa attctccatc
     1261 ttttacgaat tcattcaaat attgagagac aatattacga ctaacattta atttttcaga
     1321 tattttctgt gctgtattgt tcccccattt atttttaatc tcagagttct ctatgacata
     1381 attgacaatg cgttctttta attcttccat cacagcacct cctcgacact attcgaattt
     1441 tgccgacagt atttttccat aaatatgact tgctaaaaag gctttgttta gatcattcaa
     1501 ttggaacgtt gcttgttttg cttgatccgc taagacgttg aaaagatact ctggcgaaat
     1561 cgcatacccc aaaatatcaa cgattgttgc aatcagatct tcttttgatt cttcatctga
     1621 aagtaattca cgatacaaca tcgtttgttt gcttactgtg tcatattcct ctaaggattc
     1681 atctgctaaa agaaccactt gttccaacaa acggtcggat aaatatttat agaaaatcag
     1741 ccccaataaa taatttttat attctgacgc atccatttta ctacggagat tatctgccgc
     1801 actaaataac ttctgattta attctgcacc caaaaagata cactcctatc tcttatttaa
     1861 agttgctata taccctctta ttttagcata ctcagtttta gaaaaagcaa aaaaataaag
     1921 aaacttattc ttgataacaa gctacgtgac gtatatccct aacacaaaaa tagccatatc
     1981 ctttggtttg gatacggcta tttgatatta ctcattcaat tatcaaatca cagtaggtgt
     2041 gaatctcatc gggatcggca atatctactt ctgattagga tcaaaggtga ttttctcaat
     2101 aaaggtgtga tacaaagctt taacttgctt cttctcactc tggataagaa agcaatccaa
     2161 actagtcaat atctgttgga catctttgac ttgaatcggt tccccttgtt gttgaaagat
     2221 ccctaagatt tcattctctc gaatttgtaa ctgtcttcgt ttttcagtca attcatttaa
     2281 acgatctttt aacatcggaa acaattcggg actatcttcc aaagcagctt cctgtttttg
     2341 ctctaatagt tagattttgc gtaccatttt cttcgtttat tttccttcga ttgctgattt
     2401 taatatagtc taaaggaaat ttatatttat tgtattcttt ataacttcaa gagaacttcg
     2461 tttgagtatt actgtatttt tttaattcca taagaaaatt agttattacc tgccacctaa
     2521 tcagcaaatc tgaacctgag tcaatataat taatattatt t
//
LOCUS       DAYYGO010000196         2536 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000196 DAYYGO010000000
VERSION     DAYYGO010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.204"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            151..387
                     /locus_tag="ACHA5T_002775"
     CDS             151..387
                     /locus_tag="ACHA5T_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFM8888290.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            406..621
                     /locus_tag="ACHA5T_002776"
     CDS             406..621
                     /locus_tag="ACHA5T_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HFM8888291.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(1324..2076)
                     /locus_tag="ACHA5T_002777"
     CDS             complement(1324..2076)
                     /locus_tag="ACHA5T_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8888292.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(2091..>2536)
                     /locus_tag="ACHA5T_002778"
     CDS             complement(2091..>2536)
                     /locus_tag="ACHA5T_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFM8888293.1"
                     /translation="RYLKNIKNEYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPI
                     FDCIRVLGIILDNAIEASIESQDRELSLAIYRDDKQIEISISNSCREILLSIDTLMKK
                     GITTKKGHQGLGLSSIEEINKKNPNMFVNYQNDPYQFTTQVILLF"
BASE COUNT          823 a          402 c          415 g          896 t
ORIGIN      
        1 tcttgaacac taactttttc attcctgaat caaatttatt ttggaaattt tactattcaa
       61 gaataaactt gataagtttc aggaagattt ttactgttaa tcaaatacgt atgttacagt
      121 agcaccgtaa aaaatattat ttaaagaagc gtgtttaaac atgcaaaatg taaaggaact
      181 aagtattgtg gaaatgcaca aactgttggt ggaggcaata atgcttgggg aaaattgggg
      241 caagttgtag gtggacttac cacaggggca gtaggtggtg caggtttagg aacagcaatt
      301 tgcggaccag cttgcggtgt tgttggaggg ttatatggag ctgtcgctgg aggtgctgca
      361 gctggctggg atgctcgaaa aaaataaaaa aggagaataa gtagtatgca tattaaaaac
      421 acaaaaacaa catttatatt atcttcagaa gagttgaaaa atattcaagg tgggagtgcc
      481 gtgggagttt taggcactac tttttctggg gcaacagcag gagtaaaact ttgttcagcc
      541 ggaggacctt atgcaatagc tgcttgtggt gtaggtggag ctttattagg tgctggattc
      601 agcatgtgga ccggtgcgta aacaattaaa aaatatcaaa cctcaagatt ggtttgtgtc
      661 gattacgaac ataggtatgt tctttatctt ttcaaaagtt tggaatggac agaacttttt
      721 actaaagctt cttgtgctag tttttataat tatattaaat ttttatcttt tattaaagtc
      781 agataaaaat tctttggaaa tgtaatggta gagatatttt tacaaaagga cttgcagcgc
      841 tgtagttggt gctgcaggag gacctatttc ggccgttaca ggaggtattg gtaatgtact
      901 tgtcggatgt gcattaaagc ctgttcctgc aagttaaatt tataaaaaca ggaaagtaat
      961 tatctcaaat cctatcaagt acaatcatag gattgcactt gacaggacaa attttttaac
     1021 taaaaaatga aaaacacttt ctaaaaatta gtagaatttg ccaaatcttt cgcatcttcc
     1081 accatacatt taattttaat tcccctttat ggtacttaat aaaaacaaga aaagaactat
     1141 tcatttggcc cttttttggt tccagaccat agaaatattc ctccagtaac taatattgct
     1201 gttatgcacg taactaataa cagctataaa aattggtaca actttaatag gattcagaaa
     1261 aatttgcact agtgaaggaa tctattttat tagaaaaatg caatctccct aaagaaaaaa
     1321 ccactaaaca gtgctttctt tcaacttctc cttaattttt tgtgctttcc ctaaagtaaa
     1381 gtttctggcc aactctgaat caaaatatag tgaacgtttt ttaaaatcta tttctttaac
     1441 gttaaagata tttactaaac acgctcgact aattcttgtt aacataggat atttcttttc
     1501 aagctcacta attcttccat aaaattcgta ctgcccatct ttagtacaca atgataatct
     1561 atgtggtaat tttgaagctt ctacaaaata aatgtcattt atatcaaaag taaatgtttg
     1621 agaacctatg gaaaaaacaa atgcttgatt cgtaacctgt tttgttgcat ctatcctttc
     1681 ttgcgctaat aacaataact caacaatctc gtttcgatac tcatctagtg tttgatcttt
     1741 tgttacaaat cctaacgttt caactcttct ttttattgtt acgggtaaca tctcatcatg
     1801 agtagttgta aaaatgattt ttgcttgaac atcatatttt ctgattactt ctgctaattc
     1861 tataccgtta acttcatgat ttaaatcaat atctaagaaa tatattcctt gctttggtcg
     1921 gaattgtttg aggtattttt taacttctaa cggactttgt gttttcaata ctatcttaaa
     1981 tactttatcg tgaaaaagaa tgaaattatc aataattcgt tctaactgat ttaattgtat
     2041 aatttgatct tcacataata ttattggata actcataaat cctccttccc tcaaaacaac
     2101 aaaataactt gagtagtaaa ttgatacgga tcgttttgat agttcacaaa catattggga
     2161 ttctttttat tgatttcctc tatactagat aaacctaacc cttgatgtcc cttttttgtt
     2221 gttattcctt ttttcattag agtatctata gagagtagta tctctctaca actattcgat
     2281 atcgaaattt ctatctgttt atcatctcgg taaatagcta gagacagttc tctatcttga
     2341 ctctctatag atgcttcaat tgcattatcg agaataattc caagcaccct aatacagtca
     2401 aatattggaa caggaacttc aataatagta cttggacatt cgaatttaca acaaatctta
     2461 ttgttatttg cttggtatag cttcgctatc aacaaacttt tcaaatattc attttttata
     2521 ttttttaaat aacgat
//
LOCUS       DAYYGO010000197         2484 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000197 DAYYGO010000000
VERSION     DAYYGO010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.206"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            16..201
                     /locus_tag="ACHA5T_002779"
     CDS             16..201
                     /locus_tag="ACHA5T_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888294.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            706..900
                     /locus_tag="ACHA5T_002780"
     CDS             706..900
                     /locus_tag="ACHA5T_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888295.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            1091..1340
                     /locus_tag="ACHA5T_002781"
                     /pseudo
     CDS             1091..1340
                     /locus_tag="ACHA5T_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1584..2414)
                     /locus_tag="ACHA5T_002782"
     CDS             complement(1584..2414)
                     /locus_tag="ACHA5T_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFM8888296.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
BASE COUNT          743 a          437 c          493 g          811 t
ORIGIN      
        1 aggaagtgtc tatgaatgag taaaaattct aaaggattcc taaccatctt attagctttt
       61 atagggtata tgttggttgg gctgctaaaa tcatacagta atgagttgct gaatttttca
      121 acatttatta atgatacatt agttccaagt ttgtttttca ttgtattttt tgctgttggt
      181 tattttataa taaagatatg atgactaatt tagctcaact attccagcgt cagctgaaga
      241 tattgcaatt cccgattcct taatcattta tctggatgag acagaaagtg aagttgcaac
      301 agatgtgcat gtagttgagg ataaaaatga tagtttaaaa attaatagtt cagatacttt
      361 actaagagta agtcctatct tttcagctgt tatttagatg caagtagcaa ctaagttagg
      421 tagtactcct gcaatggata ggtatttgtg tgttcgatat ataagtggtc gaagtatcaa
      481 ggttatgtat actggatggg agatagaaga gttaaaaatt ggaggtcacc gccacaaatg
      541 gaatttagat ttagaggtac cttagatttg gtatagttat taaacacaag atggcctggt
      601 ttgtagggaa agtcgaagag tatttttcca cttctgcgcc cctttaattt ttctagtaat
      661 gccgtgaatt tgtgattaat tggaaatatg aagaaaggag agtctatgaa aacaacacta
      721 gttctattct ataaaaagca tccttatttt acacttctga taaatatatt gttggctagt
      781 gttatcggga tttctgttga atatctaata aataaagatt tcataggttc ttgtttttat
      841 acggctttat ttcttggact tcttgaagca ttttccattt ataaaaaatc aaaaaaataa
      901 aatagattgc cgtaagagat ctttttcatt tcattttttt ctgaaaaccc gttgtttctg
      961 tcagtgaagc agcgggtttt tagatttatt gtctttagac ctaattgagc gcgccgagcg
     1021 attgaaatac aaaaccactt gctatgcggg tggagcaata aagggaaata gtttagcccg
     1081 tacaaagtag atgtgtaaat accacattgt attcacacca aagtatagaa gaaaaataat
     1141 ttataatcaa tatcgagcca atattcagga gatcatcaag agattgtgca aatctaaaat
     1201 ataaatttgg gtatcgacac ttttgggtgg aaggatacta tgtgacaaaa gtaggacaaa
     1261 atgaagcgac catcaaaaag tacattcaag aacaagaaaa acatgatagt gcgttagata
     1321 agctgagtgt acgagagtaa gacgatcctt ttaagggcta aggagtagta cataaacctt
     1381 ttaagaggtg ggcaaaactg gcaaaggcaa tatggcttga acataaaata tgaaagccag
     1441 cgtctttagg cgctggccgg tgatttgagc ttatagctct agagaaaacc acccttttga
     1501 agggtggtta tgattaatga ctttagtcag ttttgctcac aaagcgtgtg gaacaaaaaa
     1561 cgggtgtttc tacgggtaga tatttatttc ttgaatggag gtgtcccata catttttggt
     1621 gttactggtt ctaattttgg tgcagcgcct tcgatcttag gttctttcaa ccattgatat
     1681 tcgccttcgc catccagtgc tttgccacta gcccaagaac cttcactact ttggtctccc
     1741 tctgagaagt tatagaagtc ataagcaatg tcttttcgtt ctttgtcacg cgggaaagta
     1801 cttgggacta agattgaacc ttctttttct tctaattctt tcactgcaca ttcccataga
     1861 ttttgatgat agctatctct tgcaaggaga acagacagca tatcttttac gccacgatca
     1921 tcgatcattt cgtaaatacg tgctacttga agtcttcctt gtgattcggc atttaaatta
     1981 gctctaaagt ctgctaacaa gttgccactc gcaatgatat aggcaccgct ccatggattg
     2041 cccacgctgt cagcaggacg aggtcctaag ccagctacga tcgcgtgctg tggattcatt
     2101 ccagagataa tggcaccgat tgttggatct ttttgcatcg ctgcttcttg atcttcaaca
     2161 ggcgcaccct ctaataaacg agcaaccatt gtcgccaaca tttccacgtg gccgatttct
     2221 tctgttgcaa tgtccatcag catatcttta tatttttctt ctcctcggca gctccagcct
     2281 tggaacaggt actgcatagc tacactcatc tcgccatatt ggccgccgat cagctcctgc
     2341 agatatttcg cgattaatgg attcggacga tctggttttg cttcaaactg caattctttc
     2401 tggtgtctaa acatcaaaat tcctcctaat ctatttttaa tacattctca ttctgctacg
     2461 aaagattaaa aaattcaaag aaag
//
LOCUS       DAYYGO010000198         2478 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000198 DAYYGO010000000
VERSION     DAYYGO010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.207"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            152..1252
                     /locus_tag="ACHA5T_002783"
     CDS             152..1252
                     /locus_tag="ACHA5T_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM8888297.1"
                     /translation="MAKYYNKKILIMANNDDGLYRFRGMLIKNLVDQGNEVIVSLPKG
                     KNIPKLIELGCQIIETPIDRRGINPKTDFKLFFNYCNILKKIKPDMVIAYTIKPNIYG
                     GLACRLRKVSYALNITGLGTAFQKKGVLKSLIVFLYRLAAKKSKVIFFENFENMKTFQ
                     TLQIAKQSKYKLLNGAGVDVEHFSYLEYPKDEQVHFLFIGRIMKEKGVDELFEAMKNL
                     YSEEKNCVLDVLGYFEEDYGIKIEKYEKEGWLKYHGYQSDIRPFVERAHCFVLPSWHE
                     GMANTNLESAASGRPIITSDIPGCKEAVIESKSGYLCERKNSCSLYNSMNKFLKLSYE
                     QRINMGKMGRNYMIKKFNKKKVVDETIKSLFS"
     gene            1259..1654
                     /locus_tag="ACHA5T_002784"
     CDS             1259..1654
                     /locus_tag="ACHA5T_002784"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8888298.1"
                     /translation="MFCKVCDRKEGFCGFRIDIKELAKVSDIFLFTTLQEGMPRSMME
                     AMASGLPCIASKIRGNVDLLVEGKGGYLVPAKDAELVAECLRELINNKSLRKYMGNEN
                     LSRIKEFEVSIVKKRIKEIYEEVIGESVK"
     gene            1651..>2478
                     /locus_tag="ACHA5T_002785"
     CDS             1651..>2478
                     /locus_tag="ACHA5T_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8888299.1"
                     /translation="MKNPMFSIIVPIYCVEKYLKDCLDSIRNQTFKNFEAILVDDGSP
                     DECPEICDYYSQIDGRLKVIHKKNGGIIDARKTGVTSAMGEYIVFLDGDDWLANTYLE
                     KYAEVITKYNPDIICSGYFASRSNKIRKRELSCSFGYYDKDKIINLIYPKLIEDEHGE
                     YFRPQLWAKAFRTTLYKEQQQVSGQVSVGEDHACTKPCIYHANSIYMMEKCLYYYRIN
                     ENSITKKPQPYYWEGPKAIGEHFEKQIDMNKYDFQDQVYRNVTHNLFNVAVSQFNKQD
                     "
BASE COUNT          984 a          272 c          468 g          754 t
ORIGIN      
        1 gtataaatca aagcgagtat attagaatta tataacatat gttaaaaaaa cgtatagcta
       61 ataaaagtat gattttaaaa atttgtgtag agttttaaat atactgtttc aatacgagaa
      121 aattataaaa aagtataagg aaaagagagt tatggcaaag tattataata aaaaaattct
      181 tataatggca aacaacgatg atggtcttta ccgttttcga ggaatgctca taaaaaactt
      241 agtagatcaa ggtaatgaag ttattgtatc tcttccaaag ggcaagaata ttcctaaatt
      301 aattgaactt ggatgtcaaa ttattgaaac tccaatagat agaagaggaa ttaatccaaa
      361 aactgacttt aaactatttt ttaactattg taatattttg aaaaaaataa agccagatat
      421 ggtcattgct tatactatca aaccaaatat atacggtgga ttggcatgtc ggttaagaaa
      481 ggtttcttat gcattaaata taaccggatt aggaacggca tttcaaaaaa agggggtttt
      541 gaaaagtctt attgtatttt tgtatagatt agcagctaaa aaatctaagg tcatattttt
      601 cgaaaatttt gaaaatatga aaacatttca gactttacaa attgctaaac aaagtaaata
      661 caagctatta aacggtgcag gagtagatgt agaacacttt agttatttag aatatcctaa
      721 agatgagcaa gttcattttt tatttattgg aagaattatg aaagagaaag gtgtagatga
      781 actttttgaa gcaatgaaaa acttatatag tgaagagaaa aattgtgtac ttgatgtcct
      841 tgggtatttt gaagaagatt acggcataaa aattgaaaaa tacgaaaaag agggatggtt
      901 aaaatatcat ggatatcaat ctgatattcg tccctttgta gaaagagcac attgctttgt
      961 tttgcctagt tggcacgaag gaatggcaaa tactaacctt gaatcagcag catcagggag
     1021 accaattatt acaagtgata tcccagggtg taaagaagca gttattgaat cgaagtctgg
     1081 ttatttatgt gaaagaaaaa atagttgtag tctctataat tcgatgaata aatttctaaa
     1141 attatcatat gagcagcgca ttaacatggg aaaaatgggg agaaattata tgattaaaaa
     1201 atttaataag aagaaagttg tcgatgaaac aattaaatca ttatttagtt aaaaagatat
     1261 gttttgcaaa gtatgtgata gaaaagaggg attctgtggc ttcagaatag atatcaaaga
     1321 attggcaaaa gtttctgata tttttttatt tactacatta caagaaggaa tgccaagatc
     1381 tatgatggaa gctatggcaa gtggattgcc atgtatagca agtaaaatcc gaggtaatgt
     1441 agacctttta gtggaaggaa aaggtggata tcttgttcca gctaaagatg cagaattagt
     1501 agcagagtgt ttgagagaac taatcaataa taaatctctt agaaagtata tgggaaatga
     1561 gaacctttcc agaattaaag aattcgaggt tagtatcgta aaaaaaagaa tcaaagaaat
     1621 atatgaagaa gttataggtg agagtgtaaa atgaaaaatc caatgttttc aataatagta
     1681 cctatctatt gcgttgaaaa atatttgaaa gattgcttag attcaataag aaatcagaca
     1741 tttaaaaatt ttgaagcaat actagttgat gacggtagtc ctgacgaatg cccagaaata
     1801 tgtgactatt attcccaaat agatggacgt ttaaaagtaa ttcataagaa aaacggtgga
     1861 ataatagatg cacggaaaac aggagtcact agtgctatgg gagagtatat agtttttcta
     1921 gatggtgatg attggcttgc aaatacttat ttagaaaaat atgcggaagt aattactaag
     1981 tacaatccag atataatatg tagtgggtat tttgcatcac gttccaacaa aattagaaaa
     2041 agagaattgt cttgctcgtt tggatattat gacaaagata aaattataaa tttgatttat
     2101 cctaaattaa ttgaggatga acatggagag tattttagac cacaattatg ggcaaaagct
     2161 tttagaacta ctctgtacaa ggaacaacaa caagtatctg ggcaagttag tgttggtgaa
     2221 gatcatgcat gtacaaaacc atgtatttac catgcaaatt cgatatatat gatggaaaaa
     2281 tgtttatatt attacagaat aaacgaaaat tctataacaa aaaaaccaca accatattat
     2341 tgggaaggac caaaggctat tggagagcat tttgaaaagc agattgatat gaataagtat
     2401 gattttcaag atcaagtata tagaaatgtt acacacaatt tatttaatgt tgctgtttca
     2461 caatttaata aacaggac
//
LOCUS       DAYYGO010000199         2410 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000199 DAYYGO010000000
VERSION     DAYYGO010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.208"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..1675)
                     /locus_tag="ACHA5T_002786"
     CDS             complement(62..1675)
                     /locus_tag="ACHA5T_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8888300.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(1816..>2410)
                     /locus_tag="ACHA5T_002787"
     CDS             complement(1816..>2410)
                     /locus_tag="ACHA5T_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8888301.1"
                     /translation="EITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSI
                     HLLSLLVTYCLQKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRY
                     LEEHLSENFLLEQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENEL
                     TYTEIADILGFHSIHHFSSAFKKYTGLSPSKYYLSLK"
BASE COUNT          736 a          447 c          430 g          797 t
ORIGIN      
        1 catttttact cctcaagtaa aaataactcc tagaccgcgg ctacccaccg cggtcgtttt
       61 tttattcttc aatcaaaatc gctttgatac ccaaacctgc caagattcgg gcagaagtat
      121 aagtattcaa tcctcttctg aaagtaatgt aaactttttt atcttttggg atttcattga
      181 ttcgatctcg taactcattc attggaatat tttttgttgc cgagatactg ccagaaggtg
      241 ctttattagg ttcacgtata tctaaaaaga acgcttcttt caaatcgttt tcgggtatat
      301 cacttgcttt gatcgtttct acgatattcg tcatttgatt gattgcgaca taaccagcta
      361 tattcaacgg gtcacgggta gtagaatacg gtggagagta aggcaactcc aaatcaggca
      421 aatcaaagac tgtcaagttt ccggtgattg ctacagaaag ttctcccatc cgtttatcga
      481 cacctttttc tccaaatgct tgtccaccta aaatacgacc gctatctgca tcaaaaatca
      541 gctttagatt taatcttgta gcttctggat agaaataggc atggtcaaag ggagtgataa
      601 aaacagtttt ataatttgtt atgcctgatg cttttaattc atgttctgtc attcccgcat
      661 aacttgctgt atgatcaaag atttttgcga cacctgcacc aatatatccg cgatagcgca
      721 taggcgtacc atttaaaata tctgccagca tatgtccttg acgattggcc gcactggaaa
      781 gcatactttg gatcggctga ccagtcacta cactagtcgt ttcgataata tcgccaatcg
      841 catagatgtc cggcagattg gtttgtaact ggtcatcaac aatgatctga cctgtatctg
      901 ataattgtat gccggttgct ttcaccactt cattcttcgg agaaacacca acagcaaaaa
      961 ttagcatatc agcagaaagg acggagccat ctgataatac tatttctcga cctttatcac
     1021 ggatctcagt aactcttgtt tctaaatgaa cggctaatcc ttctttcagc aatttgtcat
     1081 aaactaaatt agcaatttct ggatcataag ggaaagcgac ttgtggcaat tgatcgatta
     1141 aagtcacgtc taagccgcga tgcttgaaat tctcagctaa ttccagaccc ataacacctg
     1201 caccaagaat gacgacatgt tgtggatttt tttcttcaag gaacgatttg attcgatccg
     1261 catccgtcac actacggagg acaaagccgt tttctgcttc aaaaactccg ggaatatccg
     1321 gataatctgg tctagcacca gaagagatga tcaacttatc gtaatctgtt tttatttctt
     1381 catttgtgga taggtcttta actgttaatc gcttagtaga aggatcgatt gctgttactt
     1441 cgtgtttagt aaagacatcg atgttgtttt ttgttttcaa tatttctggt gtacgttcga
     1501 tcagcgagtc acggtcagtg atcacacctc ctaaataata aggcaaagca caacttgcat
     1561 aagaaatatt ttctccgcgt tcatagataa tgatttcgtg cgcttcattc aatcgacgga
     1621 aacgagtggc aaatgaagga ccgccagcta caccgccaat gacaacaact ttcataaaaa
     1681 catctccttt tctacgttga tcgtactatg ttatggtaaa aaacagcaaa taattgtgcc
     1741 ggaaaattat tgtttatttt gaatagacag aaaatataga gaaataattt gtggataaaa
     1801 cctaaaaatt tttttctatt ttaaggatag ataatacttt gaaggggata accctgtata
     1861 ttttttaaaa gctgaagaaa aatgatggat cgaatgaaat cctaatatat ctgcaatttc
     1921 tgtataggtt aattcatttt ctagcacgag acgttttgct ttttccattt ttagttcttt
     1981 aaaatagcta ataacacttt ttccattcac ttttctaaaa tgttttttta agctggctgg
     2041 actgataaaa aaaatttggg ctaattgttc aagcaaaaaa ttttctgaaa gatgttcttc
     2101 taaatatcgt atgattggat caactaattc atctgaataa agaatatgct tttttttagg
     2161 aatgactttc tcatttgaag gtattgtcaa taaagcaagt tcctgttgat aatgccttgg
     2221 taaggaagac aattgaattt tttgcagaca ataagtaact aataaggaaa gcaaatgaat
     2281 gctcatctgt tggcatagta tgaaaaaaga attgatttcc tgatcatagg aaatagctgg
     2341 cttactatgt tttagataat caattttgaa ctgttcattt ttcagaaaag taatgataga
     2401 tgttatttct
//
LOCUS       DAYYGO010000200         2400 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000200 DAYYGO010000000
VERSION     DAYYGO010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.209"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            207..2069
                     /locus_tag="ACHA5T_002788"
     CDS             207..2069
                     /locus_tag="ACHA5T_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8888302.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2400
                     /locus_tag="ACHA5T_002789"
     CDS             2069..>2400
                     /locus_tag="ACHA5T_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8888303.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          866 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
//
LOCUS       DAYYGO010000201         2369 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000201 DAYYGO010000000
VERSION     DAYYGO010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.210"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1192
                     /locus_tag="ACHA5T_002790"
     CDS             <1..1192
                     /locus_tag="ACHA5T_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFM8888304.1"
                     /translation="SDADKYNPEGQKVTTELNKEPDASEGIKNKEDLPKDTKYTWKEK
                     VDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEGEKVEVGGTVDL
                     TDNVTNLPTLPEGTTVTDVTPDGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDN
                     RSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGIV
                     VVTYPDGSKEEVEVTISVEDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPNGSQ
                     YHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNEKDSEKAVSKDN
                     KTDNQGSKQNKNRGKSSPQKQSSKAYPKTGEIDSNIFTISGGLILLGTLGLLGYKNRK
                     KENE"
     gene            1326..>2369
                     /locus_tag="ACHA5T_002791"
     CDS             1326..>2369
                     /locus_tag="ACHA5T_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8888305.1"
                     /translation="MKKIVIIGSNHSGLAAARFLNLCDSNYEVILIDKKSEISYLECG
                     TSLFIKREVSNLEDLFYQNRRELNSKIKKLFLETAVDKIDIQNKKIICVKKDGTRFSQ
                     EYDKLILATGSKQIMLDVPGNNLANIFSIKNFPNAKEIFKALDDNTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGKKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHIDNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTAR"
BASE COUNT          932 a          321 c          467 g          649 t
ORIGIN      
        1 atctgacgct gacaaatata atcctgaagg tcaaaaagtg actacagaat tgaataagga
       61 accagacgca tctgagggaa ttaagaacaa agaagattta ccaaaagata ctaagtatac
      121 ttggaaagaa aaagtagatg ttagcgcagc tggaaataaa aaagggactg ttgtagtgac
      181 atatccagat ggatcatctg atgaagttga agtagatgtt acagtgacag acaatcgttc
      241 tgacgccgat aaatatgagc caacagtaga aggcgaaaaa gtagaagttg gaggcacagt
      301 agatctaaca gataatgtta ctaacttgcc aacattacca gaaggaacaa cagtaacaga
      361 tgtcactcct gatggtacga ttgataccaa cacaccaggt aattacgaag gggttatcga
      421 agtaacgtat ccagatggta cgaaagatac agtgaaagtt ccagtagaag tgacagacaa
      481 tcgttccgat gccgataaat acaatcctga aggacaaaaa gtgactacag acttaaataa
      541 agagccagac gcatctgagg gaattaagaa caaagaagat ctaccaaaag gaactactta
      601 tacttggaaa gagaaagtag atgttagtac agctggaaac aaaaaaggga ttgtagtagt
      661 aacatatcca gatggatcta aagaagaagt agaggttact atttctgtag aagataaaaa
      721 agcaccgaat aaacctcaag ttgatcctat tacagagggt gaccaaattg ttactggtaa
      781 aactgaacca aatgcagagg tgacagttac attaccaaac gggagtcaat accatgggac
      841 agctgataaa aatggtaatt ttacagttaa agttcctaaa ttagaggcag gaacaaaagt
      901 gatagtaact gcaactgatg aatctggtaa tactagtgaa cctactaatg tagttgtttc
      961 ttcaaacgaa aaagacagtg aaaaagccgt tagtaaagat aataagactg acaatcaagg
     1021 tagcaagcaa aacaaaaata gaggcaaaag cagtcctcaa aagcagtcct caaaagctta
     1081 tcctaagacg ggagagattg atagtaatat ctttactatt agtggtggtt taatcctgtt
     1141 aggaacttta gggttattag ggtataaaaa tcgaaaaaaa gagaatgaat agaaaatcaa
     1201 tttcttaaat cttctctgaa aagaggaaga atctttttct tcctcttttt gttttttatt
     1261 ttgggagata ggttgttagt taaaaatgaa gatacttttc atagaaggga aaagaatgtg
     1321 attaagtgaa aaaaatagta attattggat caaaccattc ggggctagct gcagctcgat
     1381 ttctaaactt atgtgattca aattatgaag ttattctgat agataaaaaa tcagaaatta
     1441 gttacttaga atgtgggaca tcgttgttca taaaaagaga agttagcaat ttagaagatc
     1501 ttttttatca aaatagaaga gaactaaatt ctaagataaa aaagttgttt ttggagacag
     1561 cagttgacaa aattgatatt caaaataaaa agattatttg tgtaaaaaaa gatggcacaa
     1621 gattttccca agaatatgac aaacttatac tagctacagg ttcaaagcaa attatgttag
     1681 atgtaccggg gaataattta gctaatattt tttcgataaa gaattttcca aatgctaaag
     1741 agatttttaa agctttagat gataatacta ttaaaaaagt aggaattatt ggagcaggat
     1801 atattggagt tgaaattgct gaagccataa gaaaaagagg gaaagaagta tatcttttcg
     1861 atgttgcaga tagagtgttg tcgacttatt acgaccgtag tttttcagat aaagttgaag
     1921 aaatactatc aaaaaatgga atacatcttt gtctatcaga aagtcctaaa aaatatttag
     1981 gaagaaaaaa gattgaaaaa atagtcacta caagtggtac ccaatactct atggatatgg
     2041 tcgtacaagc tataggattt ctatctaata gtccaattgg caaaaaggaa ctcttgagag
     2101 atgttagtgg agcatatcta actaatgaat accagcaaac aaatataaaa gatatatatg
     2161 ctattgggga ttgtgctaca acttttttta cgtccattaa tagaagtagt gttgattttt
     2221 ctatttctaa tgctctaaga acagcttata ttgcaactca acatattgac aatcaagatt
     2281 ttacaccttc tggttcccaa tttacaaatg gcttttctat atttaattac aatttttacg
     2341 cagcaggttt gaatttaaaa acagcacga
//
LOCUS       DAYYGO010000202         2340 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000202 DAYYGO010000000
VERSION     DAYYGO010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.211"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(365..1657)
                     /locus_tag="ACHA5T_002792"
     CDS             complement(365..1657)
                     /locus_tag="ACHA5T_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM8888306.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
     gene            complement(1931..2191)
                     /locus_tag="ACHA5T_002793"
     CDS             complement(1931..2191)
                     /locus_tag="ACHA5T_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888307.1"
                     /translation="MKKIDLINMIGMLIGILVNIVIFTDWLGVLFSNLIPILIIGICG
                     IILSILELFESRNTMNRIFACIILIVNLLPMVYFTFLYFALG"
BASE COUNT          781 a          441 c          355 g          763 t
ORIGIN      
        1 tatgggctac tctgtgatac ttattagcca ttaaactatt ttacccacat agcatatatt
       61 ttgaaatcgt tcattccgta aattcaacta aatatgccta aaaaaataaa aaatctaaga
      121 tagaataaga tttattcagt cttagatgga tttatagcaa ctttttcgta acaactttaa
      181 aaaatacgaa aagaaagagc tgttttactc tgactacgtt gctaaattgt cgtttaatca
      241 ttcatttaaa ccaaatcttt ttttatagaa ttcgttttaa tcctcctgaa tccaaaaact
      301 atgaaaaccg taattaaata gttaagagtt tttaaccacc ataattctct cggctatttt
      361 gaaatcagat aaattttgat cggacacata gttgaataaa cctaccgttg ttacggaagg
      421 taaatcgcat acttttcatt ctagaggaga aggtctcgtt gatcattaac tgattaagca
      481 ccatagccca gttttgaatt cgaccacctg accacttcgc gtgtaattct ttcgtgcgta
      541 aataaagtag ttttaggagt gcattctcat tagagaacgc tccttttttt gtgacttttc
      601 tgaagctgga gtggacactt tcaaccgcat tggtagtgta cataattttt cgaatggcac
      661 taccataatc aaatagttgt tcaacatgtg caaagttccg tttccagaca tctacagcac
      721 cagaataatg agaccaccga ttttgaaaac tgtcaaaagc agcatgtgca gcgtttagag
      781 aagaagcacc gtagaacttt ttcatatctc ggcagacttc cttatagtcc ttacttggaa
      841 tatagcgcaa tgcatttcga acaagatgaa caatacagcg ctgaacaatt accgatggaa
      901 agatcgcttt tgcgccttct tcgaggccag aaacaccgtc catcgaaatg aaaaagacat
      961 cttcgacacc acgtgctttc agttcatcaa atacttgcat ccagcgattt ttagattctg
     1021 tttgatttaa ccataatcct aaaatctcct tatttccttt gagatcatag ccaagaatgg
     1081 tgtatacagc atattctttg gcttcataat tttctcgtaa agtaacatac atacaatcaa
     1141 cgaatagaaa ggcataacac tttgctaggg ggcgggcttg ccattcttcc aattcaggaa
     1201 ggacagcgtc agtgatatct gaaatcattt catgggaaat atcaaagcca taaatagctt
     1261 cgacggttgc ggcaatatct cgttgactca ttcctcgtgc atacatggaa agaaccttcc
     1321 cttcgatgtc ggagacatct cgttttctct taggaattaa ctctggttca aaggaagctt
     1381 cccggtctct aggaacatca atagctactt caccaaaact ggttttaagc gttttagttc
     1441 catagccatt tcgacggtta tcgtgttcct taggctcttt agaatgggca tcataaccta
     1501 aatgattatt caattctcct tgaagcattt tttcaaaaag gggcccaaac acatctttca
     1561 aagcatcttg catgtcatcg acagattcag gttgataggc attcagaatg gattcagcta
     1621 acttttcggc atcaggattt cttttctttc tagccatcgt gtaatcacct cttgatctta
     1681 ttgtagaaaa agaaaaaccg cgaagcaacc acctgcctag gattaatggt tacttacacg
     1741 gtttatatta cactctcatc attaaaaaag tatctttcga tattactaaa caatgaaatt
     1801 atctatttga aatttaaatc tgataatata ttgagtgttc caatgagggc tttgatccta
     1861 ttatccatat ctttttcagt tttaatattg atacacatta taagaaaaag aaacttttgg
     1921 ttaatttatt tcatcccagt gcaaaatata aaaaagtaaa atagaccata ggcaatagat
     1981 taacaattaa tataatacaa gcaaatattc tattcattgt atttctgctt tcaaaaagtt
     2041 ctaatattga aagtataatt ccacaaatgc ctataataag tatgggaata agattagaaa
     2101 ataacacccc gagccaatct gtgaaaatta ctatatttac taatatccct attaacatac
     2161 ctatcatatt tattaaatca atttttttca ttagatattt ctcctttatt aaatctgaag
     2221 tacttatatt aaaataattc taaactattt acctcaaaaa gtgcaataaa ttatttctga
     2281 acaataaaat gatagtcaaa acaaaaaaat attgttaatc gttcagagcc atccgagagt
//
LOCUS       DAYYGO010000203         2313 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000203 DAYYGO010000000
VERSION     DAYYGO010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.212"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            191..445
                     /locus_tag="ACHA5T_002794"
     CDS             191..445
                     /locus_tag="ACHA5T_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8888308.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="ACHA5T_002795"
     CDS             446..763
                     /locus_tag="ACHA5T_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFM8888309.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1322..2257
                     /locus_tag="ACHA5T_002796"
     CDS             1322..2257
                     /locus_tag="ACHA5T_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888310.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          846 a          313 c          434 g          720 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaaggcaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accgtaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1141 gaggaagtcc aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat
     1201 acatccctgc tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa
     1261 tgggataaaa tataggtaca ataactattt gatgctaaat ataattaatg gaggagagat
     1321 agtgaacata cttaatgaaa aaagatatgt atggtttgct ggtaaaaaat atattactac
     1381 tttcaaaaga ttatccgagt taatgcagca gactgttaaa agtgctatgc ctgaaggata
     1441 caagtttaat tttgttcaat cttcagtatc tactgaaagt atctattttt caattctttc
     1501 tgagagtagg ctttttcatt ttagaataag ttctcatgta aagaaaactg atgttgatat
     1561 atatacgttc cgtacaaata attataaaaa tttaaatgat ttgagtagaa gtatcagtga
     1621 atatctcaag gggaatagct atctcaagat tcgattttca catatattaa ttttactacc
     1681 atatttgaat gagaatttca ccctaattca agtagataag tctttcattg cctcaggtaa
     1741 tcaaggaaat atgaatatta caagtaacaa aacgtcacgt gtgctattgg cgttgatggg
     1801 ttacggaatt ctttatagag atgaccaaaa tggccagatt ctaataaatc gggctgggcg
     1861 ttcattatta atgaaatata gaaaatttat accggaagat tttcaaaact tacagttaga
     1921 aaacatgcgt ttagaagatt tatttagatt agcagaagat gataagtata gatttgaagt
     1981 tttgaaaatt caaaagaatg aactattgaa taagttatat cctgagaatg cgataaaagc
     2041 acattttggt gatgctaatc atatgtatag ttggttcatt cctaaaagat ttagagggaa
     2101 aatcaatgct ggagatcaag tgaaagtact caatcgaaat agtacagatg tcgtaatgat
     2161 aacagatatc tatgtagaag atgaagatat aatcaaatat atgaagcctg tgataaaact
     2221 tgttaataaa aataaaggaa cccgtggtgc tagttaattt tttcctagag aaaaatgtct
     2281 aaacatgttg gtaaatcaac aaaaaaacgc ata
//
LOCUS       DAYYGO010000204         2273 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000204 DAYYGO010000000
VERSION     DAYYGO010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.213"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..>2273
                     /gene="esp"
                     /locus_tag="ACHA5T_002797"
     CDS             5..>2273
                     /gene="esp"
                     /locus_tag="ACHA5T_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFM8888311.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLVGVGLVLGTT
                     ELVQAQDEISPSTPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEEPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSASAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGKKVADLAIERRKDHENYGVGSITDNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSILDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDI"
BASE COUNT          822 a          349 c          465 g          637 t
ORIGIN      
        1 aataatggtt agcaagaata ataagagagt atttctagaa aaaacaaaga aacgagtatt
       61 gaagtacagt atcaaaaaat taagtgttgg ggttgcatct gttttggttg gtgttggtct
      121 tgtcttggga acaactgaac tagttcaagc acaagatgaa atttctccaa gcaccccact
      181 tgaaacagcg ataagctccg tacaagtagg tgacaaagta gcttcgggga atacttttca
      241 ggagaatcct ggttatacga aaaattataa cttttcggat ttacaattta gtcctcaaga
      301 actaactgga gacacactaa aaggcaatac aattggattt gaagtttacg gaaaacataa
      361 tattgcagca tcaactaaaa actgggaaat ccgtcttcaa ttagatgaac gattagctaa
      421 atatgtagaa aaaattcagg tcgatccaaa gaaaggaatt ggatcttcta gacggacctt
      481 tgtaagaatt aatgattcgc tgggtagacc tacaaatatt tggaaggtca attatattcg
      541 tgcaagtgat ggattatttg ctggggctga aacaactgat acacaaactg ctcctaatgg
      601 ggtaattacg tttgaaaaga gcttagatga aatttttaaa gagataggca tagacaatct
      661 caagaccgat cgtttaatgt atcgaatcta cttggtaagt catcaagacg atgataaaat
      721 tgtaccaggt attgatagta cgggctactt tttaacagat tcagatgatt tctataatag
      781 cttagatgta tctgaaaata atcctgacca gtttaaacat ggatcagtaa gtgcaaaata
      841 tgaggaacct aatactcaaa caaaagatgg ttcaggatcc acaggtgcta atggagctat
      901 aatcttagat cataagttaa cgaaaaatta taatttctca tattcagctt ccgcaaaggg
      961 cacgccttgg tatgctaatt acaaaattga tgaacgatta gttccatatg tagcgggtat
     1021 acagatgcac atggttcagg ctgacaaagt aacatatgat gtttcttttg aatcagggaa
     1081 aaaagtggct gatttagcaa ttgaacggcg caaagatcat gaaaattatg gtgtaggttc
     1141 aattactgat aatgatctta ccaaacttat tgattttgct aatgcaagtc cacgtccagt
     1201 cgttattcga tatgttttac aattgactaa gccattagat gaaatcttag aagatatgaa
     1261 agcaacagca caagttgaag aaaataaacc atttggtgaa gatttcatct ttgattcttg
     1321 gttgtcggat acgaataaaa aattaatcca gaacacttat ggaacaggtt attattattt
     1381 gcaagatatt gatggtgatg gaaaccctga cgataaagaa gagagcggag acacgaatcc
     1441 atatatcggg aaacctgaat tagaagaagt atatgatgtt gacacaacag ttaaggggaa
     1501 agtattcatc cacgagttag cgggaacagg tcacaaagcc caacttgttg ataaagaagg
     1561 tactgtatta gcagaaaaaa ctatcgctcc aaatgaaaaa gatggggctc caatttcaga
     1621 tactgtagaa tttgaattta cgggtgtaga ttcaagtaaa ttaatcgcga aagatgaatt
     1681 aaaaatccaa atcgtttctc caggttttga taaaccagaa gaaggttcaa ccgttattaa
     1741 ggaatcacca aaagcggttg ataaacaaac cgtggtagtt ggatttaaac cagatgctaa
     1801 agaatcaatt cggaataata aaaacttacc tgaagatgca gagtattcat ggaaaacaga
     1861 gcctgatact tctaacgtta ctgatagtac gaaaggtatt gtaactgtta agatcggaaa
     1921 tcgaactttc gacgtggatg tagagtttgc tgtaaaagct tctcaagcta tggaaaatga
     1981 tgcaacatac gtacctataa caacaactcc agaaacgaca gttcaaagtg gaaaacctac
     2041 atttgataaa ccagatgttc ctttagctaa agatgccttt tcaattttag atgtctataa
     2101 taaggacttc ggtaatgcaa gtgttgatgc aaatactggt attgttacat tcactccagc
     2161 taaaggtgta ggagaatcgg agccgattac tggaacaatt cctattaaaa ttgtttacca
     2221 agatggttct gtaggcacga ccgatttagc agtaactgta agtaaagata ttt
//
LOCUS       DAYYGO010000205         2271 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000205 DAYYGO010000000
VERSION     DAYYGO010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.214"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..189)
                     /locus_tag="ACHA5T_002798"
     CDS             complement(<1..189)
                     /locus_tag="ACHA5T_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8888312.1"
                     /translation="MESGLSHFEKVGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFT"
     gene            complement(524..754)
                     /locus_tag="ACHA5T_002799"
     CDS             complement(524..754)
                     /locus_tag="ACHA5T_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFM8888313.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLTKLRYEDYLDIGFQLTDKGKKFIDDYKKRF"
     gene            complement(769..1719)
                     /locus_tag="ACHA5T_002800"
     CDS             complement(769..1719)
                     /locus_tag="ACHA5T_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFM8888314.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(1716..>2271)
                     /locus_tag="ACHA5T_002801"
     CDS             complement(1716..>2271)
                     /locus_tag="ACHA5T_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM8888315.1"
                     /translation="GITFSSFLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITI
                     GVSVLVLVTVVLFFKELLITSFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGT
                     ILIVAMLITPAATAYLYANSLKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTA
                     AFLFVISFLISPKQKFLRKKERKL"
BASE COUNT          730 a          426 c          288 g          827 t
ORIGIN      
        1 ggtaaactct tcattataca tattaataag cgcattaata tgcggctgaa taatttcttc
       61 aaataacgct tccttagaag taaaatgttt ataaaaagca cccgtagtta cgcctgcggt
      121 caagcacagt tgccttatct tagtttgctg aaatcctact ttttcaaaat ggcttagtcc
      181 actttctatt aatttttggt gagttgtaat ataatctctg gtttctatgg gtacttcctc
      241 ctaataagat gaattaacta tattatctta cacaggactt gaagtttcaa tcgttaacgt
      301 ctattactga actcattcct tagagctaaa ctaatgagaa agagcaaaat aactgataca
      361 aaaagcaatc ctacaagaat taatagactc atctaaatca ttcacctttt ctaaaataat
      421 ccgagaaaga tttaaaaaaa ttatatccag ctattaggat aagtcctatc ataatcactc
      481 caatagccat tttcttatcc tccctaaaaa taaagccttt tacttaaaac ctttttttgt
      541 aatcgtcaat aaattttttc cctttatcag ttaattgaaa tcctatatct aaatagtcct
      601 catatctaag tttagtcagc atctcactta cagatgctgg actaacactt agaacttttg
      661 ctaaatcatt attttttaat ttcttttttc tttttgataa ttcctgaatt gctagtaaat
      721 aaattagtat agtagttgtt ttaatttgta ccatacctac cctccaaatt atttcgaaag
      781 gccttcagca attttatcca ggttccattt catcatcgca tagtaactat caccatcttg
      841 tcctttttct gcaattgaat cagtaaaaat cgttgaatag ataggaatat tggtatcttt
      901 tgatactgtt ttcattggtc tatcgtccac actgctttct acgaataagg agggaacttt
      961 tgttgtgcgt aatttttcaa ctaagtgttt tatttgatct ggtgttcctt cttcttcagt
     1021 gttgatttcc caaatgtaag cagaaggcac attatacgct ttcgagaaat atttaaagca
     1081 tccttcactt gtaacaatca tttttttatc attcggaatt gaagcaaatt tagatttagc
     1141 ttctttgtct agagaatcca atttttcaat atacttatct agattttctt tatagaattt
     1201 tttattatca ggatcttttt ccgctaactg tttttcaata tttttagcgt aaataatacc
     1261 gttttctaaa tttaaccaag catggggatc ttccttccct ttctcactct gaccctctaa
     1321 gtaaataaca tctatgccat cacttgctgc aaaatagtct ttgttttcct ctttgttcgc
     1381 attttttact aattttgtaa accaagcatt tcctccagtt tccaagttaa caccgttata
     1441 aaaaatcaaa tctgcttttg aagttttttg aacatcttca ggcaaaggtt catattcgtg
     1501 gggatctttc ccaataggta cgatactgtg taaatcaatt ttatcttttg ctatattttc
     1561 agtaatatct gctaaaatcg aattagtaac tactaccttt aatttttcgt tcgaagccac
     1621 tgtatctttg tctttactat ttgttgaaca tgccccgaat ataaaaagag caatcaaaat
     1681 ccccagactt cccagtactg ctttccattt tctcatcata actttctctc cttctttctt
     1741 aaaaattttt gttttggtga gattagaaaa ctaattacaa ataagaatgc cgctgttaaa
     1801 acaatacttg atcctgctgc tatgttaaaa ctataaccaa tgaacaaacc tagcaatgat
     1861 gagcacgctc cgactgtcgc cgaaataaat atcatagttt ttaaactgtt tgcatataaa
     1921 tatgctgtag cagctggtgt aattaacatt gcaacaatta atatagttcc cacactttgc
     1981 atagctgtta ctgctactaa agttaataga atcattaata gataatgata aaaactgaca
     2041 ttcatcccca ttgcttttgc cattaatgga tcaaaagaag tgattaataa ttctttaaaa
     2101 aatagaacaa ctgtaaccaa cactaaaaca gaaacgccaa ttgtaatcca cttatcaata
     2161 tcttgtacag ctaaaatatt tccaaataag atatgaaata aatccgttga gctatttgca
     2221 actcctataa ggataactcc taatgctaaa aaagaactga aagtaatacc a
//
LOCUS       DAYYGO010000206         2205 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000206 DAYYGO010000000
VERSION     DAYYGO010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.215"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..479
                     /locus_tag="ACHA5T_002802"
     CDS             <1..479
                     /locus_tag="ACHA5T_002802"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8888316.1"
                     /translation="KEIKELVNVYREHKPDINFLENADTLLTELRSRGYKLGIITDGY
                     AITQRNKLDALKVDYLVDYICITDELGRELWKPHPISYLLLLEKFNLKAQECIYIGDN
                     ELKDFITAKSIGMQTIKIERVNGIYRESSLKFKRFYQADKKIRSLWEIKNFLGVIS"
     gene            476..1594
                     /locus_tag="ACHA5T_002803"
     CDS             476..1594
                     /locus_tag="ACHA5T_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HFM8888317.1"
                     /translation="MKSKRILLASPHMSEEGYEKEYVKEAFDTNWVAPLGANVNGFEK
                     ELAEYVGASHAAALSSGTAAIHLALKAAGVGKDDIVICQSLTFSATANPILYQGAIPV
                     FVDSDYETWNMKPEHLRTALEKYPNTKAVLVVHLYGLSANLDEITKICKEYDVTLIED
                     AAESLGTTYKGKYTGTFGDYGIYSFNGNKIITTSGGGMLVSNDKEKVEKVRFWATQSR
                     EKARHYQHNEIGYNYRMSNIVAGIGRGQLKVLNERIEKKRYIHSFYKKELSKLSNIQF
                     MPENNWNEPNYWLSVITIKGINANHLIDELDKNNIEARPVWKPMHLQPVFKNYDYIGD
                     DVAETLFDLGVCLPSDTKMTDEDLKYVVSTIKRVINDA"
     gene            1584..2204
                     /locus_tag="ACHA5T_002804"
     CDS             1584..2204
                     /locus_tag="ACHA5T_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFM8888318.1"
                     /translation="MMRKKTFYQLFGKRILDILLSGIALIVLSPIILIVGFLVRIKLG
                     SPIIFKQERPGKSEKIFSMYKFRTMTDERDHNGEYLPDEIRLTKFGKMLRATSLDELP
                     ELWNILKGDMSIVGPRPLLVEYLPLYSEKQRKRHNVRPGLTGLAQVNGRNAISWEEKF
                     DLDVYYVDKISFFNDLIIIIQTCKKVIKKENINTINNNIPEKFKGS"
BASE COUNT          835 a          286 c          432 g          652 t
ORIGIN      
        1 aaaaagaaat taaagaactg gtaaatgtat atcgagaaca caaaccagac ataaattttt
       61 tagaaaatgc agatacttta ttaacggaat taagaagtag aggatacaag ctggggatta
      121 ttacagatgg gtatgctata acccaacgaa acaaattgga tgctttgaaa gtagactatt
      181 tggtagatta tatttgtatt actgacgaat taggccgtga attgtggaaa ccacacccta
      241 tctcatatct tttattgtta gaaaagttta atttaaaggc acaagagtgt atatatattg
      301 gcgataatga gttgaaagat tttataactg ccaaatcaat aggaatgcaa acaattaaaa
      361 tagaaagagt aaatggtatt tacagagaaa gcagtttgaa gttcaaaaga ttttaccaag
      421 ctgataaaaa aatacgttcg ttatgggaga taaaaaattt tttgggagtg atttcatgaa
      481 aagtaaaaga attctattag cttctccaca tatgagtgaa gaaggatatg aaaaagagta
      541 tgtaaaagaa gcatttgata caaattgggt tgctccttta ggtgcgaatg tgaatggatt
      601 tgagaaagaa ttagcagaat acgtgggagc tagtcatgct gccgcattat cttctggaac
      661 agcagctata catttagctt tgaaagcggc tggggtcggt aaagatgaca tagtgatttg
      721 tcagtccttg acgttctcag ctactgctaa tccaattctc tatcaaggag ctatacctgt
      781 atttgttgat agtgattacg aaacttggaa tatgaaacct gaacatttgc gaacagcact
      841 agaaaaatat cctaacacaa aggctgtatt agtagtacat ttgtacggtt tatcagctaa
      901 tttagacgaa attactaaaa tatgcaaaga atacgatgtt actctaattg aagatgctgc
      961 agaatcatta ggaacaacat ataaaggaaa atatactggt acatttggcg actatggaat
     1021 ttattctttt aatggaaata agataatcac aacttcaggt ggcggaatgc ttgtatccaa
     1081 tgacaaagaa aaggtggaaa aagtgagatt ttgggctacg caatctcgtg agaaggcacg
     1141 ccattatcaa cacaatgaaa ttggctataa ttatcgaatg agcaatatcg ttgctggtat
     1201 cggtcgagga caattgaaag tattaaatga aagaattgag aaaaaacgat atattcatag
     1261 tttttataaa aaagaattgt ctaaattgtc aaatatacaa ttcatgccag aaaataattg
     1321 gaatgaaccg aattattggt taagtgtcat aacaataaag ggaataaatg cgaatcatct
     1381 tattgatgag ttagacaaaa ataatataga agcccgccca gtatggaaac ctatgcattt
     1441 acaacctgtt tttaaaaatt atgattatat tggcgatgat gtagcagaaa ctttatttga
     1501 cttaggagta tgtttaccat cagatacaaa gatgacagat gaagatttaa aatatgtagt
     1561 atctacgatt aaaagagtga tcaatgatgc gtaaaaaaac tttctatcag ctttttggga
     1621 aaagaatttt agatatccta ttgtcaggaa ttgctctcat cgttttatcg ccgataatct
     1681 taattgtagg ttttttagta agaattaaac ttggttctcc aattatcttc aaacaagaac
     1741 gaccaggaaa aagtgaaaaa attttttcca tgtataaatt tcgtacaatg actgacgaaa
     1801 gagaccacaa tggagaatat ttacctgatg aaattcgact aactaaattt gggaaaatgc
     1861 ttagagcaac aagtctggat gagctacccg aactttggaa tattttgaaa ggtgatatgt
     1921 cgatcgtagg acctcgtcca ttattagtgg agtatttgcc actatatagt gagaagcaac
     1981 ggaaaagaca caacgtaaga ccaggattaa ctggactggc tcaggtcaat ggaagaaatg
     2041 ctattagttg ggaagaaaag tttgatttag atgtttatta tgttgataaa attagtttct
     2101 ttaatgatct tattattata attcagacat gtaaaaaagt aatcaagaaa gagaatatta
     2161 atacaatcaa taacaatatc ccagaaaaat ttaaagggag ttaaa
//
LOCUS       DAYYGO010000207         2204 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000207 DAYYGO010000000
VERSION     DAYYGO010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.216"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            546..1577
                     /locus_tag="ACHA5T_002805"
     CDS             546..1577
                     /locus_tag="ACHA5T_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8888319.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            1686..2111
                     /locus_tag="ACHA5T_002806"
                     /pseudo
     CDS             1686..2111
                     /locus_tag="ACHA5T_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
BASE COUNT          787 a          379 c          353 g          685 t
ORIGIN      
        1 ctatatgaat ttttatgaaa aatttcatat aatttccact cgttgtgatg gtttaattta
       61 tccaatccat caataagcaa acaacttata taaaaaagga ttaattctca atataattgc
      121 tcctaccata gaataaatct tcatgtaaag taatgaagta ccaactattt ctaatacttc
      181 tttaagaatt atcccaacaa tatcgttaga caaaccatta ttgaataaat ggagagccaa
      241 tcgattctga aaaaatgttt tctaattttt cggaaataaa agaaagagcc tcctgccatt
      301 agaacgaacc aaatccattt cattgctcat gaagtaacca catagctaaa aagaggataa
      361 atcaattaaa agatcaaaag aatgactgca ctaataaaat aactaaaaat gtatcttctt
      421 acttcatatc ttttagctag tacttagtta aaaatttgct tcctaagatc aagacaaact
      481 gtctatcttc atttgatgaa atgcggtaaa atatagaaaa tgaacactat tggagggaat
      541 ttgtaatgga cagcaaaact agaaaacaac tgcaagaaaa gttaaagcaa cgtacacact
      601 tgctcccttc cgtctttcaa cagtttttag aagaaaatcg ttcttccctt tcattaggca
      661 gtcgctatga atacgcaaaa gattttcatc tgtttactga ttatttgaga aaagtatttt
      721 ctgaagaagt ggatgatcag ctgattatcc atttagaaaa acaagattat cagaatttct
      781 tagctaaaat ctatcgacat actcgttcgt tccaaactac agcgaaccaa gaaacggagc
      841 aattgtttga aaatagctat tacgggattt caaggaagca atattcatta aatcattttt
      901 tacgattttt gtatcaaaaa ggactgattg aagaagagat ttctcttttg ggtacaagtt
      961 taccggaaac aaccaaagca gctccgcgat atatcgatgc agaacttttc aaagattttg
     1021 agcatttgtt tatcccgatt gaaggctttc aaacaagcag agaagctagc tttgaagaaa
     1081 agaatagacc gaggaacctt gcaattactg ccattcttct gtattgcgga ttaaagatcc
     1141 atgaagtagt agaacttaaa cgtaaagatg tatctttgac caagcagatt cttaatctga
     1201 atagaaaaga aaacagactg aataaagtac caataccaaa agaagcgatg ccatttctgg
     1261 agaactattt taagttaaca gcacaaaata cggacgaaaa ttcttttgtt tttcgttcct
     1321 cacatgatca gcaaatttct cctcggacta ttcgccaaat tttgagcaaa attacagtat
     1381 ataaaaacgt atcgccagaa ctatgcagaa aaacatatcg ttattatgca gaactatatt
     1441 tagatgatgc tgacgcggta aattatttat gtggtaatcg atacctctca tcccattctt
     1501 ttcaagatat ctgggaaaag atggttgatt tttcttatca cgaattagcg gcattcgttc
     1561 gggtttcttc catataaaac aaatgtatga tctttttttc acaataaact atgttattat
     1621 ataaaggatt atgttacgat tatcttgaat ttgtatatta cttaacaaaa gataatggag
     1681 tgtgaatgat ggagaccata acgtattccc ctttgaactt atttacgaat tttcaagaag
     1741 ctgcagctaa cttttccgag accccgatta tctttgatga gccactttca gctttcccag
     1801 aattaggact tgaaacaact tacaaaaaat gctgcgaaag tgtgttgaaa cgggcttatc
     1861 aattggctca tcttggagtg aaatccggag acaaagttat tatttataag agttccgctt
     1921 ttgacacata ccttctagct gtttctgtgt cttaccttgg tgcagtgcct gtcatgactt
     1981 cttatcatct accaacaaca acaatggaag tatttatcga tcgtttagaa gaccctttca
     2041 ttttatttga tgatgagaca aaaaagcgag tgagagaaat ttcaaatgga acaaaatcaa
     2101 aacagattcc taacccgtgg tgctagttaa ttttttccta gaggaaaatg tctaaacatg
     2161 ttggtaaatc aacaaaaaaa cgcatatagt agtctcaaaa ctcc
//
LOCUS       DAYYGO010000208         2192 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000208 DAYYGO010000000
VERSION     DAYYGO010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.217"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..443
                     /locus_tag="ACHA5T_002807"
     CDS             <1..443
                     /locus_tag="ACHA5T_002807"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM8888320.1"
                     /translation="LVPRIFGLTPYIIEDKEMSPYYAQGNLIYVRPKNTNEILVGDII
                     TYYENSGKKISTRRVIAVENNHEDFYTKADTKTYIEPGVVKKRNIIGSPIFQIPYIGL
                     VLSKTAFAWIQGLFFTIAFCLTGITAWNTFVELKKKRRRTQKFL"
     gene            571..1254
                     /locus_tag="ACHA5T_002808"
     CDS             571..1254
                     /locus_tag="ACHA5T_002808"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFM8888321.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1563..1636
                     /locus_tag="ACHA5T_002809"
     tRNA            1563..1636
                     /locus_tag="ACHA5T_002809"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1597..1599,aa:Arg,seq:tct)
     gene            1795..2154
                     /locus_tag="ACHA5T_002810"
     CDS             1795..2154
                     /locus_tag="ACHA5T_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFM8888322.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
BASE COUNT          733 a          341 c          448 g          670 t
ORIGIN      
        1 tcttggttcc ccgaattttt ggtttgactc cttatattat tgaggacaaa gaaatgagtc
       61 catattatgc acaaggaaat ctgatatatg tacgaccgaa aaatacaaat gagattttag
      121 tcggagatat tattacatat tatgaaaatt caggaaaaaa aatctcgacg cgtcgcgtaa
      181 tagccgttga aaacaaccac gaagattttt atacaaaagc tgatacaaag acttatattg
      241 agcctggagt agtaaaaaag agaaatatta tcggtagtcc catctttcag attccatata
      301 ttggactagt cctttcaaaa acggcatttg catggattca ggggttattc tttacgattg
      361 ctttttgcct aacaggaatc acagcatgga atacctttgt agagttgaaa aagaagcgga
      421 gacgaacaca gaaatttttg taagaatgtt aaaaacataa atgtattcca gagatccaac
      481 gactcttctg aacatgaaac acgagaaaac actcatgcag cttgtagatt tagtcagttg
      541 ttcgtataat gtgtgaagag gaggagtagt atgcgcaatg aaatgatgca tcgtcctgta
      601 gtcaaagaag aactattgga ttttatgcgt acccagcaaa aaaaattacc gggtgagcta
      661 ggcaaactgg aagaagaagc tcatgtagca gaagtaccag ttattcccca tgaaacagtt
      721 gtatttttga aatttttact tggacagctt cagcctgaac gaatcttgga gatcggagca
      781 gctatcggtt tttcatcaag tgtcatggcc acgacaatcg gagaaaacgc tcatgttacg
      841 acaattgatc gtttcgatgt catgatcgag aaagctaaaa aaaactatga acgtctagga
      901 ctgacagata aagtgacttt gcttgaaggt caagcagcag atatcttgcc ggagttgtct
      961 ggtccgtatg actttatttt tatggatagt gcaaaatcaa aatatattga atttttacct
     1021 gaatgcttac gtttgttaag aaaaggtggc gttttgatgg tagatgacat cttccaagga
     1081 ggaacgatct tgctcccaga tgaagagatt ccccgtggca aacgagctat ccatcgtaaa
     1141 ttaaatgaat ttcttcgtgt tgtgatggat catcccgatt tgacatcgac aatcttgcca
     1201 ttaggtgatg gtgtgatctt aatgacaaag gaatcagaaa caatcgagtt gtgatacatg
     1261 attatggcag cctgtagata atcgattgaa tcgagggctc aattcgaact tgatgtaaaa
     1321 gtttctatat ttgtaagtca attaaaggat acaggaaatc taaataaaca ggagtagcat
     1381 gaagtcaatg tagctcctgt tttcaattga taaccttaga acagctgaag gagaatgtct
     1441 cgttttaaaa gattgattta gtagttgggc caaaataatt cattatttgc taattaaata
     1501 actatttcgt attgacagaa gcaatttaat tatctataat tattgatgtt ccgctgattc
     1561 ttgtcatagt agctcagctg gatagagcat ccgccttcta agcggacggt cgggggttcg
     1621 aatccctcct gtgacgctaa aaaaccgtta gaaactgagg tttctaacgg tttttctttg
     1681 ttttttgtgc caatattgtt tgttttcaaa ggcaaataaa ataaaaaaac atccttatcg
     1741 agtacaatta taaggagcaa aaaaataaaa ctcatcagaa aagaggaaga agatatgacg
     1801 atactatcta gaattttagt gactctagtt gcaattgaat ttttttatat catgtatatc
     1861 gaaacggtga gaactgattc tgacacgaca agtagggttt ttaaaatgag taaagaagag
     1921 ttaagcagaa aatctgttca aacattgttt aagaaccaag gagtgtacaa tggactatta
     1981 ggagtgggac tactttatgg agcttatctt tcttccgcat ctaaagaaat cacaagtatg
     2041 ctattaatat ctattttttt tgttgcatta tatggcagtt tgactagcga tcgttggatt
     2101 ttactcaaac aaggtgggct agctctgttg gcattgcttt ctattttctt ataaatatag
     2161 aaaaaataag aatttatcta acaggaaact tt
//
LOCUS       DAYYGO010000209         2174 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000209 DAYYGO010000000
VERSION     DAYYGO010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.218"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..157
                     /locus_tag="ACHA5T_002811"
     CDS             <1..157
                     /locus_tag="ACHA5T_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HFM8888323.1"
                     /translation="NKIQDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKW
                     IQKQIAY"
     gene            130..807
                     /locus_tag="ACHA5T_002812"
     CDS             130..807
                     /locus_tag="ACHA5T_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFM8888324.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            758..1939
                     /locus_tag="ACHA5T_002813"
     CDS             758..1939
                     /locus_tag="ACHA5T_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFM8888325.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIF
                     K"
     gene            1952..>2174
                     /locus_tag="ACHA5T_002814"
     CDS             1952..>2174
                     /locus_tag="ACHA5T_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8888326.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITH"
BASE COUNT          790 a          286 c          445 g          653 t
ORIGIN      
        1 gaataaaata caagatcctc agtttataaa aagcttgaaa aatgttaaga acatctacgg
       61 tgacggcaac agttctagaa gaataacaaa aacattgaat caaatattga atgatgggga
      121 agatattaag tggattcaaa aacaaatagc ttattaaaat atactagtga tccatacatt
      181 attattgggg ctggcggaca cgcaaaagtc attattgaaa ccttaagatt agaaaataag
      241 ttaatatatg gattttttga tgataagcaa aataaaatgc aagatctgaa atatcttggg
      301 aaaataaacg aattgaatag ctgggcggat agcaaaaaaa aaatctataa atttattgta
      361 gctattggtg actgtgtagt aagaaaaaag attataaaaa aattgaatat gccaagtgag
      421 caatatggaa tagctgttca tccaagtgct atagtggcaa aagaaatttc aattggttat
      481 ggaactgtta tttttgcgaa tgctgtaatt aacccagatg ctgtaattgg agagcatgct
      541 ataataaaca caggtagcat tgttgagcat gattgtagaa ttaataacta tgttcattta
      601 tctccaggtg tatgcttagc gggtggagta catgtaggtg taggaactca aataggaatt
      661 ggttctcaat gtatccagat gaagaaaatt ggatcttggt gtatgatagg cgcagggagc
      721 actattgtta aggatattcc ctcacattcc ttagcttatg gcaatccagc taaaattaag
      781 aaagaaggaa ttaattttga attctagaat tttattatca agtcctcata tgggaggaac
      841 agaagaaaaa tatgttgcta aggcttttaa agataattgg atagcgccat taggtgaaaa
      901 tgtggataaa tttgaagaaa gtattacaaa atatactggt tctaagggcg gagcttctgc
      961 gttaagttca ggtacagctg ccatacatct tgccttaaga ctgcttaatg ttggaaaaaa
     1021 cgatattgtc tttgctcctt catttacttt tatagctaca gtaaatccta ttttatatca
     1081 aggggctgta ccagtgttca ttgactcaga acctaataca tggaatatga gccctgttgc
     1141 actagagagc gcattagaag aagctgaaag aaacaaccaa ttacctaagg caatcatcgt
     1201 agtgaattta tatggtcaaa tggctgatta tgaacgaatt ttagctattg caaaccaatt
     1261 taatgttcca gtgatagaag atgcagcgga atcgttaggt tctacttata agggaatttc
     1321 gtcaggctta tttgggacaa ttgggatcta ttcatttaac ggtaataaga tcatcacaac
     1381 ctcaggtgga ggagctctag tgtctagtga taaaaaatat gttgagcatt caagattttt
     1441 agcaacacaa gcaagagatc aagcaccgta ttatcaacat agcgaaatag gttataatta
     1501 ccgtctaagc aatatttctg ccggtatcgg acgtggacaa atggaagtat tgaatgatag
     1561 agtagctaaa cgaagagaaa ttttcaatcg atataaggaa gctttttctg gaattgatag
     1621 aattgaaatg atgccagaag tggatggcat ggtgccaaat cgttggttgt ctactttaac
     1681 gatcaatgat tataattcta ttgatcaagt agttaacgta ataaataaac taaatgaaaa
     1741 aaacattgaa acaagagctt tgtggaaacc actacatttg caaccagtat ttcaaggggt
     1801 aaaaatgtat taccatgata ataatcttag tgtgtcagaa aagttattta atagcggctt
     1861 atgcttacct tctggaagta atatgacaga tagtgaacaa gaatttgtta ttgatgagtt
     1921 taaaaaaata ttcaagtagg tgtgatattt tatgaacata ggaattattg gtttaggttc
     1981 catggggaaa agaagactta gactcattca agaacacttt aatgatattt tactatgtgg
     2041 tgttgatact aatgaggcga gacgaaagga agttgaaaag gaatttggaa taccaactta
     2101 taatagtcta acagaggcaa aaaatttgtt tgaaataaat tcagtttttg tttgtacatc
     2161 ccctataacc catg
//
LOCUS       DAYYGO010000210         2151 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000210 DAYYGO010000000
VERSION     DAYYGO010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.219"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(169..495)
                     /locus_tag="ACHA5T_002815"
     CDS             complement(169..495)
                     /locus_tag="ACHA5T_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888327.1"
                     /translation="MNKVNMNKKFIIIVIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(541..903)
                     /locus_tag="ACHA5T_002816"
     CDS             complement(541..903)
                     /locus_tag="ACHA5T_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFM8888328.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGADAVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVALAFIPVVFGAVAVIMGYLYRDKDEQMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(940..1154)
                     /locus_tag="ACHA5T_002817"
                     /pseudo
     CDS             complement(940..1154)
                     /locus_tag="ACHA5T_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            1282..1617
                     /locus_tag="ACHA5T_002818"
     CDS             1282..1617
                     /locus_tag="ACHA5T_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8888329.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(1693..>2151)
                     /locus_tag="ACHA5T_002819"
     CDS             complement(1693..>2151)
                     /locus_tag="ACHA5T_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888330.1"
                     /translation="NEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMT
                     EKELTNTVCERLRNVGNNSVENALKENRGYFVKQGFTLERASKDIKRLYNVKASSSFL
                     VYLAKGAGIDEVIKELILSGVELVPLYQFTSVTRDDGRRVRKRKDPIVLN"
BASE COUNT          652 a          438 c          301 g          760 t
ORIGIN      
        1 ttcttttata ttgttataac agtgaataac ataaaaattc ccgacaatca ttgtaatatt
       61 tttttgtcac ctgtattagt atttaattta taggaaacta taaaaactcc ctagtgaatt
      121 tacacctttg ggagttcctg tttggacggt atgcataata catttaaatc aatttaacat
      181 taacacctca gcatatggta tctccacata tatacggtct ttctttggat agtactcaat
      241 atttatgttg atagcacttc cattgtctaa tatagcttta gcccggtatg tgttatctcc
      301 attttcatga gtaatcgtca catcatcaca tgatcgattt aagttgtact gttcttgtaa
      361 aatctgtgtt accaatggtt tcgatgactt ctctaatcca gatgtacctg tcatttttga
      421 aaaaatgata cctataatag ccaagacaac gataactata atgataaact tcttgttcat
      481 atttacttta ttcaactttt tatctcctat ctccaaaata atttgctctt atccctctta
      541 tcagtacatt gacatcccta aaagtacacc aaagattgct ccagctaccc ctgctatcat
      601 caaaatcgta cccatttgtt catctttatc tcgatataag tagcccataa taacagcaac
      661 agcaccaaat acaacaggaa taaatgctaa cgctactacc atagaaatcc aaccaataat
      721 caaatacagc ttccctgtct cttgtccatg tacgatttgt tccttcattt cattaattgt
      781 ctgctcagta cttttcttta ctgcgtctgc tccagttgtc tcatctgttt cttgtagtgc
      841 tccgcaaaac tggcaatact tactttcatc tgttatttct ttgccacatt ttctacaata
      901 catgtcttct cctcctaaaa acattcaatt gtttattagt taactgttta ctttattaaa
      961 tacatcagaa aacagctcat tcattggtac acttaatatc tcagaaagca tcttcatttg
     1021 atagggttta ggaatcgttc tccctgtttg ccacgaactt acacattgct gtgtaacatt
     1081 catttttttg ctaactccgt ttgagaaagt cctttttctt ttctgtatcg gctgagatta
     1141 cttacttttt tcatgattct cccctcctgt tttattatat acaacaatat gatgtatttc
     1201 aactcataaa atataaaaaa acttcaaata ccattacaaa tacaacaact tgttgtacta
     1261 tactgagatg ggggtgtatt aatgaaacag aaacaatatg caaaggtcat tagagaaaag
     1321 cgaaaagctc tcgggtggac acaaaaagaa ttagctgaaa aaatcttctc tacacaacag
     1381 gcagtagcaa gatgggaaaa ttctgtaaca gagcctaacc tcgatagctt aaccgctctt
     1441 tctagagccc taggaacccc tgtaagccat tttctagaca atgttgtcgt agattatgag
     1501 gaagagtttt tggctctcta tcgttctctt agcactgaag atgcagttcg aacaattgac
     1561 tatatgaagc tattaaaaag gcaagaaaat gaacgcaatc agctattgaa agattgaaaa
     1621 ataaataacc caccgaactt taacggtggg tgtattgaga tgaaataaga ttaagagcta
     1681 tggataacct gtctagttta atacaattgg gtcttttctc ttccttactc tccgtccatc
     1741 atctcttgtt acacttgtaa attgataaag aggaactaat tcaactcctg ataatatcaa
     1801 ttcttttatt acttcatcta tccctgctcc ttttgctagg tagactaaaa aactactact
     1861 tgctttcaca ttgtatagtc tcttaatatc cttagaagct cgttcaagag tgaatccttg
     1921 tttaacaaag tagcctctgt tttcttttaa agcattttcc acgctgttat tcccaacatt
     1981 acgtagacgt tcacaaacag tgttagtaag ttctttctct gtcattagat atgttccttg
     2041 ccacgataag ccctgtaaaa cttcatgtgc ttcatctaaa atcaattgtt tttccttgct
     2101 aagttctgat cgggaatttt gaggaaatac tcttttagcc tctttttcgt t
//
LOCUS       DAYYGO010000211         2133 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000211 DAYYGO010000000
VERSION     DAYYGO010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.221"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..672
                     /locus_tag="ACHA5T_002820"
     CDS             <1..672
                     /locus_tag="ACHA5T_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFM8888331.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
     gene            complement(895..1119)
                     /locus_tag="ACHA5T_002821"
                     /pseudo
     CDS             complement(895..1119)
                     /locus_tag="ACHA5T_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1673..1759)
                     /locus_tag="ACHA5T_002822"
                     /pseudo
     CDS             complement(1673..1759)
                     /locus_tag="ACHA5T_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          764 a          346 c          337 g          686 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt agctaagtgg tgagtagttc acgtaggtat
      841 ttttattata tatagattta acatatgtcg gtcggttgat tgatatagct aaagttataa
      901 ctcaaccgaa gccgattcat ctacacgaat aaagttgcga gtgttctcgg ctaattcata
      961 aaaattgtta tgtgttccat atagcaatac tggaataact ttattttctt gaatctcata
     1021 agcaactcta tacacggtga ccgcatactt aaatccatta gaccaattac ctgataaacc
     1081 tccagttttt tgctcttact taaatggatc aatggcaatt tcatcacaaa cctatagagg
     1141 atgagtgact tctgcctaat tacgttaaaa aatattgaaa gtaactctat gtagcaaaaa
     1201 atatctagaa tttcatttcc tcaaaactga tcgatattgt atctaaaaat atatactacc
     1261 taaaataggt agtatatatt aaaatatgtg cttttgttag aaaaatcaat acataaatta
     1321 tcatatctgt atatgtcatt taggtatttc agttatgtta taatagtttt gctgtctgct
     1381 accccggcct aatctggcaa ggggaggagg agatataatg aaataccttt ttgattttgt
     1441 ttttgcaatc aagccacttg cagatgtagt gtgcagttac ctctgcaaat ggcttgattg
     1501 caaaattgcc aaaaaagaca gtaagcctac ccgttaattc tataatatag aaacgtaaaa
     1561 aaaaccccat cagcctgcac gctgatgggg tttggtgata taatgaaata cctctttgtt
     1621 actcataatt ataccataat tagtcacgaa tgcaaccaat agaaaaaact acttagaacg
     1681 gtagatcatc gctaataaca atgcattgtc caccaaatgg attttgtgaa gcattaaata
     1741 cattgttatt aattgtcgct gcttttaact aggcctctcc ccttctatta taaaagaaga
     1801 tatatatcat ttcttaatta cctactacct taaaccaaaa tttaagtact acctaattta
     1861 agtagtactt aaacaatcta aaaaatagtt atcccaattg ttatgctttt ataatacgat
     1921 tatttgtaac aaaatgaata ttatataatt aaacactacc atatttaggt agtgtttaat
     1981 tattagcaaa acaaaaaata tttattctat tctcttaact tcaactaagt tatgctgcac
     2041 acctttatat aaatcatatg caactcatta gctagacaaa ctacaagcat gtagttttat
     2101 ctgttaatta tgtactacct aaataaagta gtt
//
LOCUS       DAYYGO010000212         2115 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000212 DAYYGO010000000
VERSION     DAYYGO010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.222"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(78..1004)
                     /gene="cysK"
                     /locus_tag="ACHA5T_002823"
     CDS             complement(78..1004)
                     /gene="cysK"
                     /locus_tag="ACHA5T_002823"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFM8888332.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(1144..2031)
                     /locus_tag="ACHA5T_002824"
     CDS             complement(1144..2031)
                     /locus_tag="ACHA5T_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8888333.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          584 a          473 c          356 g          702 t
ORIGIN      
        1 tcatcataaa tttttgtgtg ttcttttcat ctttaaaagt tttttacata aacctatctg
       61 gagaaaaacc aagtgcctta tatctcttgg taaagggctg tagataaata acgttcacca
      121 ttgtcgggaa ccacagctaa taccttcttg ccttttccta attcttttgc gacttttagc
      181 gcggcagcaa ttgcagcgcc agatgagatg cctactaaaa tcccttcttt tcgcccaact
      241 tctctagctg tttccatcgc ttcgtctcca ctaatagaaa ggactttatc atacacagaa
      301 gtatccagtg ttttaggaac aaaacctgta ccgattcctt ggattttgtg aggtcccgct
      361 tctttccctt ctaaaatagc agattctgct ggttctaccc cgattaattc gattttcgga
      421 tacacacgtt ttaattctcg gccagcgcca gtgatcgttc ctcctgtacc gatacctgca
      481 acaaacgcgt ccaagccatt tactccaaat gcttgatgta tctcaggacc agtcgtcttc
      541 tcatgcacaa gaggatttgc ttcattttca aattgtaatg gtaaaaagta gccgttttct
      601 tttgccaaac gttctgcttc tctgattgat ccagaaatac catccgcacc aggagtcaaa
      661 atcaactctg cgccgtaccc tttcatcaat aaacgtcttt cgatactcat cgtctcaggc
      721 atcacgatga ttactttata tccttttgca actcctacca tcgacaatcc gattcctgta
      781 tttccagaag taggttcaat gatagtatca cctggtttca gtagaccatc gtgttctgct
      841 ttttctatca tacttaaagc aatacgatct tttacacttc ctcctggatt aaaaaactca
      901 agctttacaa aaacatctgc tgaatcttct ggtacgattt tatttaattt gacgattggc
      961 gttttgccaa tcaattcggt gactgagtta tagatagctg tcatcttcaa ttcctccttg
     1021 tctttgctct tcttacttat cataatacaa tctgttcatt tatggcgagt gattcgtctt
     1081 tgactagcaa aaaaaagaca aagatatacc atatatggta atttctttgc ctttttatct
     1141 aaatcaaacg tttagttctt gaccaactaa taaagaataa ctggacatag acatgccatt
     1201 tttcgccatt agatcatcaa cagacatgcc aaatttttgt gcaatatccc atagcgtatc
     1261 acctgacacg actgtatatg tctgaccatt atttgtattt acatctgttg ttgaagatga
     1321 tgacgttgaa gtcgtcgtag tagtcgttgt tgttgtactt gtggttgttg tagccgaacc
     1381 tgttgatggc gtatcaaaac gggttaaatt ataagtatta atcaatgagt tcaatttggc
     1441 cccataattt ggatcggtag cataacgacc tgtcaagtac gctgttgcat cataaaaact
     1501 tgttgtattg cttttccaca cgccagcata atgataatcg cccgtggaaa aactggttga
     1561 acgaagaaca ttcgcatggt cttggaagga ttcccaataa gaagaatatt ttcggaatgg
     1621 ctcattcatc gttacccatt gtccgtttac atattctttg gttgccatat aaacgacctg
     1681 tccgttatag cttcctttca caccgaatag attataattt ggcgcactag ctaatccgga
     1741 agtgccatat gcactttcca acaacgcttg agcgatcatg actgaagcat acaagtcatt
     1801 acttgcagca acttctgacg cagaccagcc gatttgatcg ataaacgctt gaggatcagc
     1861 gactgtttga gatccttgtt catctgcatg aacatgttgc ggtaccgctg taaccatcat
     1921 cggcccaact aaaagactcg ttccaattaa aggagcaatc gcttttttat tgatcaccat
     1981 ttttctttta ggtctatggt gccgggcact gcgggtcttc agctcttgca ttcaaattcc
     2041 tcctcaaaat aaaaaaactt aataaaaagt tctcttctta aaacaaactt aattaattaa
     2101 attatacttt taatt
//
LOCUS       DAYYGO010000213         2080 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000213 DAYYGO010000000
VERSION     DAYYGO010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.223"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>2080
                     /gene="acm"
                     /locus_tag="ACHA5T_002825"
     CDS             <1..>2080
                     /gene="acm"
                     /locus_tag="ACHA5T_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFM8888334.1"
                     /translation="SMLFIITNITSLIPVHVYADAGRDISSNVTSLTVDPTNITDGGN
                     IKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVGTLTVRKDSA
                     QVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATVNVTKPASGS
                     SSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRLDPSTFEINQ
                     IHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRKIMVSYKTII
                     ENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGELKIFKYIND
                     TEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVKEKEAPNWID
                     FDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPSIYFKLFRAS
                     TNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGNDYVPSGYQK
                     IENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQHKEVSEKDD
                     WRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNISIPGSHITP
                     WKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITNKSKSILPKT
                     SSKKSSFAVII"
BASE COUNT          817 a          343 c          403 g          517 t
ORIGIN      
        1 atcaatgtta tttattatta ctaatataac atcactgatt cctgttcatg tctatgcgga
       61 tgcaggcaga gatatcagca gtaatgtcac ttcgttgacc gtggacccaa cgaatattac
      121 agatggagga aacataaaag taaaattctc gtttgacgaa aaaaaacaaa atatccaacc
      181 aggtgattat ctttggatca attggccaag tgaaggaaat attcgcggtg aaggattcca
      241 aaaagaaatc ccattaatga ttgaaaataa aaatgttgga actttaacgg tcagaaaaga
      301 ttctgcgcag gttgtgttta atgaaaatat taaaaaccta gactcagtag aaggttgggg
      361 gcaatttgag atacaggcca gaaacgtaac cgataccggt gaagaaaata cgggaccatt
      421 caccgtgacg agcggtgata aaacagctac tgtaaatgtg acaaagccag cttctggttc
      481 ttcttctagc gttttttatt ataaaacagg agacatgcta cctgaagata ctaagcacat
      541 tcgatggttt ttgaatatta acaataacgg cacttatgtt gaacaaccag ttaaaatcag
      601 tgatgaaatc caaagtggac aaagattaga tccaagtacg tttgaaatta atcaaataca
      661 cttaggtgaa cagaaggttt atagaggaga agagggaatc caacaatttt tacaagattt
      721 ccccagcgct acatttaatt tttcagtaac tgataattac attgaaataa ctataccaaa
      781 aaactttgtc aatcttagga aaataatggt gagttataag acgatcatag aaaacccaga
      841 acaaataaat tttgagaatc attcggaagc ttggtttaaa gaatttaata aacctgctgt
      901 tgatggagaa agtttcaatc atacagttaa aaatatttca gcttctggtg gagtcaacgg
      961 tacagttaga ggcgaattga agattttcaa atatatcaat gacacagaga ttggtatccc
     1021 aaatgtgact ttcgaattaa gacgagcaga tgaacagcct attcaaggac agtctagtat
     1081 cctgttgact tcgaatgaac agggagaagc tagtatcaaa ggcctgcaag taggagacta
     1141 tgttgtaaaa gaaaaagaag cacccaattg gattgatttt gatccactaa gttcaaatga
     1201 actcaaattc tccataaacg aaaatgatac agagggagtc agtttaccta tttataacaa
     1261 gaaaaaggtt acgaacatca cagcaactaa aatttggaac ggtggaacca ctccaagacc
     1321 gagtatttat tttaaattat tcagagcaag tacaaacaat tgggaaccag ttcctgatgc
     1381 agaaacaaaa cgattagaca atggaataac ttcagtgact tgggaagata tccaacagta
     1441 tgatgatagt ggaaacgaat atacgtttaa agtgcaagaa gtagatcaaa acggaaatga
     1501 ttatgtccct tcaggttatc aaaaaattga aaacgggcta gtcgttacaa atgagaataa
     1561 agaagtcatt tcagtctcag gacaaaaaac atgggaagac aatgagaacc aagacggcaa
     1621 acgaccgacg acgatcacag taaatctatt agccgacggt caaccgatcc aacacaaaga
     1681 agtaagtgaa aaagatgatt ggcgctatcg tttcacaaat ctgccaaaat acagagacgg
     1741 gcaagaaatc atctatactg taacggaaga caacgtgccc gaatacagta cgacgattga
     1801 cgggtataac atcaaaaaca gttatacacc ggaaaaaaca aatatatcta taccaggtag
     1861 ccatataaca ccgtggaaaa attttccaga gaagacagag agagagaaaa ttagaaatct
     1921 attctctcaa aaattgcaaa tatttgctca tacaggagaa tccagcgaac aaagaagcaa
     1981 ttatattagt aagagagctg tacctaaaca gataacaaat aaatcaaaaa gcattcttcc
     2041 taaaacaagt agtaaaaaat ctagttttgc tgtaatcatt
//
LOCUS       DAYYGO010000214         1962 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000214 DAYYGO010000000
VERSION     DAYYGO010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.224"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..633
                     /locus_tag="ACHA5T_002826"
     CDS             133..633
                     /locus_tag="ACHA5T_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFM8888335.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            730..>1962
                     /locus_tag="ACHA5T_002827"
     CDS             730..>1962
                     /locus_tag="ACHA5T_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFM8888336.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMI"
BASE COUNT          636 a          266 c          446 g          614 t
ORIGIN      
        1 gagttttcca aactgattgg cttcttggtg attgctttga ttgcggtagg tctggtcttt
       61 aatgcttctg gtgtgaagga tgtcttactc aatttgttta accgaattgt cggtgcctga
      121 gggggaatca caatggaaca gatacgtgtt tatattgcga accttggaaa atacaatgaa
      181 ggtgagttgg tgggggcatg gtttacccca cccgtcgatt ttgatgaagt gaaagaacgg
      241 attggattaa atgacgaata tgaagagtac gcaattcatg attatgaact gccttttgag
      301 attgatgagt atacaccaat tgaggaaatt aatcggttgt gtggtttagc ggaagagctt
      361 aaaggaacgc caattggtga ggtcgcttca gagattcaac atgccttctt caattctttc
      421 gaggaaatgg tagagcatgt ggatgatatc atctattatc ccgattgtaa tgacatgagt
      481 gatgtagcgt attacttgat tgtagaagct ggtgatttag gcgaagtacc agaacacttg
      541 aagacgtata ttgattttga tgcgtgtggt cgagacttgg agattggcgg gaattatttg
      601 gttacgagtc gaggggtttt tgaactaatg taaatcattt gttagaattg accagaaatt
      661 tctatcgtac ttttactttt ctatttgata tactgaaata gaaaatattt gttgataggg
      721 gtgttactaa tggttggcaa taagtggtac aaatgtgatc ttcatattca ttcaactgcg
      781 agtcagtgct ttcaggataa aaaggttact gctaaggaat gggtagaaga atgtttaaac
      841 aaaggactcg attgtgttgc attaactgat cacaatacag gggaaaatat tgatgaatat
      901 aagaaagtgg ctatcgaaaa tggcttagat ttttttcctg gagtggaatt aacgtgtgga
      961 gaaaatggaa ctcatttact agtcctattt gataaaagtg catgtacaaa tgatatctct
     1021 gatttcttaa ccaagatggg tataaaaaga gaggattggg ccagcgctgc agcttgctca
     1081 atgaaatcag ttttagaagt tattgatttt gctgagcacg agggcttgct cgttattcct
     1141 gcacatatag atgagtttaa tggaatttct tcagcaaaat ttggaaatct aggaaagata
     1201 tttgaatcag aaaatatata tgcagtacag acggttcaaa aagaattttt tgaaaatcgc
     1261 tcacaaatga ttccctcatc taagcgtgac actatctatg atagtgtgaa tgccagatat
     1321 gatggtagag ttggaaaaga tactttggaa tcttggtata aatcagttgt tgaggcagaa
     1381 aaaaataata tgacatttct tagcttttct gataacccac actcgaaagg aaattcaaaa
     1441 catggacttt ggggaatagg cacaagatat tcctatataa aaatgaagga cgaacctgac
     1501 ttgggatcac ttcgcgatgc tttaatgcta ggcgaaactc gagttcattc tgatttttct
     1561 aatttctcca ttaatgaaaa tgaagtttta ttggaaaaat taagcttttc tgggaccact
     1621 cttagcacaa aagaagttgt agtcgaattc agcgataatt taacttcaat tattggagga
     1681 agaggaacag gcaaatcttg tattacaaga ttcttggtct atgtccttgg aaaggaagca
     1741 gaacttgatc aattttctga aatacaaagt gattatcaga actttgcaca aattgaacat
     1801 aatggctctg gaatattttt gaaggataca attgtgaaac tgaatatttt ctataagggg
     1861 acaaaatatc aaataataag aacacaggat agacattcta tttatgaatt tacacaagag
     1921 aatggtctgg ttgaaaaaga aacagaacgg ttgagaatga tt
//
LOCUS       DAYYGO010000215         1957 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000215 DAYYGO010000000
VERSION     DAYYGO010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.225"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..186
                     /locus_tag="ACHA5T_002828"
     CDS             <1..186
                     /locus_tag="ACHA5T_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFM8888337.1"
                     /translation="QDHSEWAYISRNLKYRLRKLLKEESQNDELTNHLRETFKESAND
                     LKIELDTWLLGLDVTEK"
     gene            386..568
                     /locus_tag="ACHA5T_002829"
                     /pseudo
     CDS             386..568
                     /locus_tag="ACHA5T_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313295.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1149..>1957
                     /locus_tag="ACHA5T_002830"
     CDS             1149..>1957
                     /locus_tag="ACHA5T_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM8888338.1"
                     /translation="MANENQLSEIQSKIEGRGAEKRTTINQLEKLNRISNRRLSVDDK
                     VHPLKLSWSIDRITIVGKLNDTIAYWTQNGVVFVDFEQLMRLNENQGYLKSVGTNGWQ
                     LFDQYGEDIAYIEMLKFHPDKGRIDFNPNKIGEFLKGSMKQFIHDLFLDAHFSRADIA
                     CDILGVPDDFITQYRIVDPVSFKPIYGRSGKLETAYWGSRASERQVRMYNKKLEQERK
                     KQIVPKEIETWWRIEMQLRRGKATDWHAMVRESLDSFASPHYLPGDVKPVD"
BASE COUNT          647 a          304 c          456 g          550 t
ORIGIN      
        1 caagatcatt cagagtgggc gtatattagc agaaatttga aatatagatt gcgcaaattg
       61 ttgaaagaag aaagccaaaa cgatgagttg acgaatcatt tacgagagac gtttaaggaa
      121 tctgcaaacg acttgaaaat agaattagac acgtggcttt tgggtttgga cgtgacagag
      181 aagtgatacc gaataagaaa agtcagatga gtgagagtaa atttacgtca atgtttaatg
      241 acgtgagttt acgataagcg attattttgc gtgaagcaaa ataaagcgta aaaggagttg
      301 agagcgtgac acgctttcgg ctcctttttt cgttgtcact tgtgacagcg aataccctcg
      361 gagagcattt ggaattgtga cagccatgtc acaattagga aaatgttact ctttgtcaaa
      421 gagtaacaga aaggtttaca ctctttttat tagcgacagc gcgaaggagg ggtttaactt
      481 ggggtttagt atttcgttca aaaagggaac aaagaataca agcatttctc ataataatcg
      541 tgagttaact gatagccaaa aaatcaatgg tttgtctggt agaaaataat tagcagattt
      601 acaagtggga aataaaaagc tgaaagaaga aaattgcatt tcaaactaca ttttctacaa
      661 tagctctaaa atggcatatt ttgcgttctg agcggtttta gcgtttattt cgtttagtta
      721 tcggcacaat cgttaaaaca cgttagagga gcgaaaatgg agcctgtaag gcgactggta
      781 gcggaacgat cgtgttagat tgtgaaaacc gatagctgaa tgaaataaac agcgggtgcg
      841 ccctttccat ttcggcagga ggaggctcaa ggggttcggg gaatgaaatt ccctgaggtc
      901 tttttatttt tttaggagga gtgctataat caaagaaaac gagaaaaaag agcaaaataa
      961 aagccgagca gtgcgctaac actctcgact atttacggaa actacttcac ctagctccgt
     1021 aactcaggtt cattatacga atatttttag gtttcttcaa gctagagatt tttggatatg
     1081 aatttttagg gttacgtgaa ctctggaaat tctttccaaa agtctctttt ttcgtctgga
     1141 ggacgaaaat ggcaaatgaa aatcagttga gtgaaattca atcaaaaatt gaaggtcgag
     1201 gggcagaaaa aagaacaacg ataaatcagc tcgaaaaact taaccgtatt tctaacaggc
     1261 ggttaagtgt tgacgataag gtacaccccc taaaattaag ttggtcaatc gatcgaatta
     1321 caatcgtcgg aaaactgaat gataccatag cttattggac gcaaaatgga gttgtgtttg
     1381 tcgattttga gcaattaatg cgtttgaatg aaaatcaagg gtatttgaag tcggttggta
     1441 caaatggttg gcaattattt gaccaatatg gagaagatat cgcctatatc gaaatgttaa
     1501 aatttcaccc tgacaaaggt cgtattgatt ttaatccaaa taaaattggc gagtttttaa
     1561 aggggtcgat gaaacaattc attcatgatt tgtttctgga tgcgcatttc tcacgtgcag
     1621 atattgcttg cgatattctc ggtgttcctg atgattttat tacccagtat cgaatcgttg
     1681 accctgtgtc ttttaagcca atttatggcc gttctggcaa attagaaaca gcgtactggg
     1741 gtagtcgtgc cagcgagcga caagtgcgga tgtacaacaa aaagcttgaa caggaacgaa
     1801 aaaaacagat tgtaccaaaa gaaatcgaaa catggtggcg tatagaaatg cagctaagac
     1861 gtggcaaagc aacagattgg catgcgatgg ttcgggaaag tttagatagc tttgcaagtc
     1921 ctcattactt gcctggagat gtaaaacctg ttgatag
//
LOCUS       DAYYGO010000216         1956 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000216 DAYYGO010000000
VERSION     DAYYGO010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.226"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            270..836
                     /gene="amaP"
                     /locus_tag="ACHA5T_002831"
     CDS             270..836
                     /gene="amaP"
                     /locus_tag="ACHA5T_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFM8888339.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            849..1037
                     /locus_tag="ACHA5T_002832"
     CDS             849..1037
                     /locus_tag="ACHA5T_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFM8888340.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1050..1607
                     /locus_tag="ACHA5T_002833"
     CDS             1050..1607
                     /locus_tag="ACHA5T_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8888341.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1639..1881
                     /locus_tag="ACHA5T_002834"
     CDS             1639..1881
                     /locus_tag="ACHA5T_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8888342.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          713 a          305 c          404 g          534 t
ORIGIN      
        1 aaatagaatc ttgtaataat aactgagcat aatctttgca atttttaatc ttgaataata
       61 tcctttaagt atagccagca atcaaaagta aatagagaat aagtaagcaa ttgttatttc
      121 agtatcagac tggcaataaa acgccgttat gatccagcaa tgtttggtag cgctttattt
      181 actgatgcta tgctaactgt tcgctatcta tactgtgatt gtcggtagag ttatttacag
      241 ggtaaaaata agtttatagg aggtggaaaa tgaataaggg gttaaaaata atcggtgtta
      301 tcatgagttt actgctactt tcagtatttc tagcgattgc gttgatcaat gcgccgtata
      361 ttatgcctga acaattggaa cgatttcgct tctttacttt aacgaattac tacatgcaac
      421 aatatatttt ctgggcagct gtagtatttg ccgttcttgc tattatcatc ttgctagtcg
      481 ttcttttcta tccaaagtca agaggaactt tcgttatgaa acgggaagat ggcaaactaa
      541 caatcgacaa aaaagcgatc gaagggttgg tacgttccca tttacacgaa gaagagttca
      601 ttcattcacc taaagtacgg atacgttcaa cgaaaaatcg aatccatatc catgtcaacg
      661 gagacttgaa acgaacttct tcattagtag ggaaaacagg tgtattgatg caagatatcg
      721 aagaagaagt aactaaagta ctcggaacaa aagaaacggt caaagtggca gtcacataca
      781 gcggttatga agaacaagaa gatacaagag actacaagca ctctcgagtg gaataggagg
      841 gccaaataat gaaagaacta ttttcagcat acaagctccc tattatcttt gggggtcttg
      901 gactactgct agctgtttta tggttaacga ttggattttt caagacattg ttgatcataa
      961 tcatgacagc aattggcgca ggaatcggtt tttatttgaa acagtcagga atagttgatc
     1021 aattttttaa atcataggag gaaacaaaga tggaaaacaa aaacatgaat acaacaaatg
     1081 gatcagtttt ggcaaatgaa aaaggagaaa accgcatgat gaatggtgaa ttgacttatg
     1141 aagacaaagt catccaaaaa atcatcggtt atgcattgaa agatgtagat ggtctattga
     1201 cattagatgg cggatttttc tcaaatatgg ctgaaaaatt agtcaacaca gataacgtaa
     1261 cagcaggtat caatacagaa gtaggtaaga aacaagtagc tgtggatcta gatgtgatcg
     1321 tagaatacgg taaagacatc gaagatattt acaaccaaat caaagaattg atcagtactg
     1381 aagtaaacaa tatgacacat ttagatgtta tcgaagtgaa tgtgaatgtt gcagacatca
     1441 aaacacaaga agaataccaa aaagatcaag aaacagtaca agacaaagta acagaagcag
     1501 caaaatccac aggacaattt gcttcacgcc aaacagataa agcaaaatct gctgtaggca
     1561 aaggtgcaca aaaagtcaaa gaaaataacg aaccacgtgt acaataagaa gtaagaagtc
     1621 agttaggagg aatttaaaat gggatttatt tgggcattaa tcgtcggcgg tatcattgga
     1681 gcaattgcag gagcaatcac gaataaagga tcatcaatgg ggatcatcgc aaacatcatt
     1741 gctggtttag taggttcagc aattggacaa gctttactag gaacttgggg accaacaatt
     1801 gcaggaatgg ctatcattcc atcattgatc ggtgcaatca ttttagtatt agtcgtttca
     1861 ttctttgcac gtaaattata agcaaattga agaaaaactt atgttgaaat gaagaacaaa
     1921 tttgagaacc gtagcgacac tcgctgcggt tctttt
//
LOCUS       DAYYGO010000217         1909 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000217 DAYYGO010000000
VERSION     DAYYGO010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.227"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            150..1451
                     /locus_tag="ACHA5T_002835"
     CDS             150..1451
                     /locus_tag="ACHA5T_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM8888343.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            complement(1555..1755)
                     /locus_tag="ACHA5T_002836"
     CDS             complement(1555..1755)
                     /locus_tag="ACHA5T_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8888344.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT          616 a          382 c          331 g          580 t
ORIGIN      
        1 cgtacaaaaa aaccgtcttt tcttggctct ataatttcta ggactgatag aaaatcattc
       61 tatcggtccc ttttgagcat gaaaaaacaa aacatacaga agctccgtta gaattgaatc
      121 accacaacac aatcaaagga gcgttctgta tgcctgatct tcatcatatc aaactactac
      181 ttggaatcaa agataaaaac attttcatca caaatgtgga atcgaagagc attaagaata
      241 tcaagagtct tgttgtttct gcgacgttaa gtaaagaaat tcgtcgctgt cctttgtgta
      301 aacagatgaa tcatgaaggc atgatcgtta agaacggaaa gaaaaaatcg ctcattcaac
      361 ttaataaatg tgcgaaccaa ctgacttatc ttgctttagc gaaacaacgc taccactgtc
      421 gtggctgcca cacctatttt acggcgaata cctacattgt cgatagaaac tgctttatcg
      481 ctaaacaagt gcgctataaa attttagaag agctaactga aaaacaagca atgacgacaa
      541 ttgctaagca ttgcggcgtt tcttggtcga ccgtttctcg gacactcgct tctctgatcc
      601 ccatgacaaa agtaaaaaga aattggttac cgcgttgttt attaatggat gaatttcgtt
      661 cactgaaaaa tcaagtcggt ccctactcct tttcttgtat ggatggcgat actggaaaat
      721 tactggatat tcttccttca cggaagaaaa aggctctcgt ttcttatttt atgcaatttg
      781 aacgacgcgc acgattgaag gtaaaggtca tcgtgacgga catgaatgtt tcttatgcct
      841 ctctgataaa ggaatgtttt ccaaaggcaa aactcgttgt ggatcgtttt cacattgtca
      901 aacatctcat tcgtaaattt gaggatattc gcgtacgaat catgaaaagc tttgatcgaa
      961 acgatccaac acaagccaaa tattatcgac aattaaaggc gttgtctcgg cttctgatca
     1021 aacgacagga caagcttgtt tatgacaaat ggaccaaatg gcgcaatttt ggttgggcgt
     1081 atctcaccga aagtgaagtg gtggatcgtc tgctttcgat ctctgacgaa ttacgaatcg
     1141 cctattttta ctatcaagaa attcttcaag cgtttcacga caaagaggcg gacactttct
     1201 ttaaacttgt aagaacgatg cccaattccg ttccaaaaga actgcattct ataaaaaaag
     1261 cttttataaa ctatgaatcc ggcattcgtt tagccctcga attgccttat tcaaatgcca
     1321 aaatagaaaa tctgcatacc cacatcaaag cattgaaacg agtcgcttat ggctttcgaa
     1381 gctttcggaa aatgaagacc agaattttct tactaaataa tctgattacc tatgaatcaa
     1441 aaaacatcta aagtagaaaa ggaattctac cttagatgtt cttatctcac tctatcagtc
     1501 ctatttgaca aagagccctt ttctttcgaa aagacggtta aaagtcaatt tttattaagc
     1561 tttaacaatg tttgttgctt gagggccacg ttgaccatct tcaacatcgt aagacactgc
     1621 ttggccttct tctaaagttt tgaagccgtc accttggata gctgaaaaat gtgcaaatac
     1681 atcatttcca tcttcaccag tgataaagcc aaaacctttg tctgcgttga accattttac
     1741 tgtaccgtta ttcataaata aattcctcct gatacgtatc aatgtacgtt ttttcgttgc
     1801 aataaatatt tgataggcaa aaggagtttt atctatgtag atatatcgct caaattacgt
     1861 ttcaaaatag attactcatt taatatatca tgctttttct aataacaca
//
LOCUS       DAYYGO010000218         1858 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000218 DAYYGO010000000
VERSION     DAYYGO010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.228"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            226..423
                     /locus_tag="ACHA5T_002837"
     CDS             226..423
                     /locus_tag="ACHA5T_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888345.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            529..1011
                     /locus_tag="ACHA5T_002838"
     CDS             529..1011
                     /locus_tag="ACHA5T_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888346.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1069..1665)
                     /locus_tag="ACHA5T_002839"
     CDS             complement(1069..1665)
                     /locus_tag="ACHA5T_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM8888347.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
BASE COUNT          581 a          274 c          370 g          633 t
ORIGIN      
        1 atattttaat atgtaggaaa taattagttt ttaaggaata tgttttaatt ttttgaaaaa
       61 aattcttcta aatacaacag ctgttttaat tgtgaaaacg aaaacagtac gctataatgc
      121 ctgtaaggtt gatttttaac tgtaaataaa aatcaatgag ttaaatctcc aaagaaatag
      181 cagtggtaat gtggagaaaa gttccagcag tttaggagga ttttgatgac ggaagaaatg
      241 attaatttag gagaacaata tgcatgcaaa ccaattggtt ttacaaaaac ggtcattggt
      301 gaagttgtat caaaaatgac aaactgcgcc gtggtcaaag ttgcccaatg tgctgcagag
      361 gaccaagaat tattagatga aaaagcaagc atggttgttg caaaatatga tacatttgaa
      421 taaagaaaac aattggacgt gcggcgtgct gcaagtccaa tttctttgag aaggtaaatc
      481 agtattcttt tcctattttc tggagaagaa aaagcgaggc gagttgtcat ggtaacattc
      541 tttacggttc tattgttttt attcgttgtt gtgatgtttt tatttctttg gctggttcga
      601 aaagaaatta tttatcggac tgtgcgaaat cgatgggtct acttggtgat acccttctta
      661 gtgtcactag tcatttggta tacactcatc agtcatccta cagcagatga attggcaaaa
      721 gggatcttgt cagcaatgat ttttattagt tttttgttgg acagtagagg aatcacagaa
      781 gaaggacttg ttttgaatag ctttgacaaa aaaggtgttc ctttttctga gatcaataaa
      841 attgtattat atcagccaaa aggttctaaa atcgtaaaaa tgaatttttt ccgaaacgga
      901 tggcgaggac cgatgctgaa attttctgca tcattagaag aattagttcc atttttgtcc
      961 caacgcttaa acgatgaggc agagattgat attatgattg atccagaata aaaacagagg
     1021 ttgcgacaag atgtttgtca caacctctgt tttttatttc aaataaaatt atccaaatac
     1081 gcggaagcga tcggcatcgt tcatgtattc tttttcctca gccggtgttt ctggtgaacc
     1141 gaatactaat tgcgaacgta gtttccagtt tgaagggata ttccattctt ttgcaactgc
     1201 ttcgtcaata actgggttgt aatgttgcaa gtttgcgccc aatcctttat caactaatgc
     1261 tacccaagta ttagctgttg cgattccgtt tgattgttct gaccaatctg ggaagttgtc
     1321 tgcatataat tcaaattgtt cttgtagacc ttttaccacg tcagtatctt tgaagaataa
     1381 tactgtacca taaccatttt tgaaaccagc taatttgttt tgtgtatttg ggaaagcttc
     1441 tgctggagtc aatgggcgaa gtgcttcttc agtgatttcc caaagttttt catgtgcatc
     1501 tccgaaaagg ataactgcgc gtgttgattg agcattgaaa gcagttgggc tttctttaat
     1561 tgcttctttg atcaaagttg ttaattcttc attagaaagt gaaacattac gtcctagatg
     1621 atagattgaa cggcggtttt tcaaagtgtc agtaaaagtt gtcataataa aatttctcct
     1681 ttgatttgcg ttttgcattt ttttaattga ttacgaaatg aagtatagca ccttacaaaa
     1741 agtaagtaaa cagatatgct cacaaaaaat aagcgaagtt caaaaaacaa ttttaataag
     1801 aaaaatcgtt atgtaaggat tttcttagtt atcgctttca ttgttgtgtt ttaggaat
//
LOCUS       DAYYGO010000219         1844 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000219 DAYYGO010000000
VERSION     DAYYGO010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.229"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            23..268
                     /locus_tag="ACHA5T_002840"
     CDS             23..268
                     /locus_tag="ACHA5T_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8888348.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            344..709
                     /locus_tag="ACHA5T_002841"
     CDS             344..709
                     /locus_tag="ACHA5T_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFM8888349.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(770..1333)
                     /locus_tag="ACHA5T_002842"
     CDS             complement(770..1333)
                     /locus_tag="ACHA5T_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888350.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
BASE COUNT          569 a          349 c          284 g          642 t
ORIGIN      
        1 tactataatg aaaggtgatt aaatgagtat cgaagaagct attttactgg atactttacg
       61 ttgtcgtctg aatttcttaa gaactggccg atctttatct caagtagctg tttctagcgc
      121 gttaagtatt cctttattca aatatcgtaa gtacgaagaa ggagaagaaa ttccaagcat
      181 cattgaatta atctatatcg ctgactttta cgatatttcc gttgattacc taattggtcg
      241 ctcagaaaca gcaagcaaac tgaagtgatt ttttccagcc ttggtgctgg ttttttcatt
      301 taacgtctaa ggttaacaat ctatatataa aggagacatt tctatgcgat tatggcatga
      361 aaaattgatt tcaaagcttc ctcgtcaaca gcttttagga caacatcggg aatgctgtgc
      421 attaagagga aatggctggg gaaagaaaca tgctactgtg gattatgttt ttcactattc
      481 tccttataga ttattccaat atcatcagct tgtcatggat gaaatggaaa aacgtggtta
      541 cagcccagca ccagagtgga aaaatcctat gtatcgtggc aaatcatgcg aagcattttt
      601 tgatttacct actgtagagc gcaacgtgcc gatttatccg gaacacgatg aagtttatct
      661 agcagaatgt ttagcaaatt tggagcaaaa aggaatttat cttacctaat ataactagaa
      721 aagccttcta tctcctctta cttcattaaa aagctttaac catcttagat caagctaaaa
      781 tgtcgtaagt tttagggtta tcttcatcct cacgtgcaaa actaaaagcg tactctagtt
      841 ttggttcatc gccaacttcc ctagacataa atgaacatct cctgacgtgg tttaaaagta
      901 ccgcatcttc gacgtctgta tacgatgcac agcacagatt gcttcttttg atctttcttt
      961 tgatttttct gttgcttctt ctacaccttc ttcgattact acatcgatgc ttacaattgc
     1021 ttctgcctaa tattcccatg catcgtcgat ttaagcctct gtagtcactg tgatcgtttc
     1081 cacattttaa ctcctctgtc gtggcttcag ttgattttgt gatcagttca gttgctaagc
     1141 cagagtcaga acaacctgcc attgctagcg ttaatattga tagtaaaaat agttttttta
     1201 tcataaggtt ccccccctga aaatagacta aaataataac gctttctttt tttgttttca
     1261 gacgaaaatt aataatttct aaatattctt taaaaaagct tataactatt atgccttttt
     1321 taatatataa cacagttatt tttaaaaata agaatgctaa atcactctac aataacggcg
     1381 aaagcacaaa aaataaaaaa attattacgt tattaaaaaa acagatgaaa gatcaggttt
     1441 tattagatct cattctttca tcacttgaag ggatttaatg aactactttt tcctctttgt
     1501 cgtttttata tattcgtatt ccttcaaaca gtaggatagc ctctttcaat gcttgatatt
     1561 tgaactgtcc gttacttaaa aatttagatg tgccgccctc ttcctagaac atattctttt
     1621 ttactttttc attaattgac agaccaatcg aacgaaactg taaatttcga taggataatg
     1681 attggtcaaa ctcattcatt ttcaagacat ctcgattcat attcaagata ggaatctttt
     1741 tatcaacatc actttattcg tattttcctc taccataaaa gcttcacgct ccacaaaatt
     1801 gttttataca ctactattca gcccattcaa cgaccaatga attt
//
LOCUS       DAYYGO010000220         1843 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000220 DAYYGO010000000
VERSION     DAYYGO010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.230"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..560
                     /gene="wzy"
                     /locus_tag="ACHA5T_002843"
     CDS             <1..560
                     /gene="wzy"
                     /locus_tag="ACHA5T_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy"
                     /protein_id="HFM8888351.1"
                     /translation="GGLSKLTLKDFTSVFDSDDSPLVDSFFEMGGTMSIITFFLISGN
                     GIYPFANTYLTSLLGSISTRMLSMFGIKFVLLADWFSQDYLGITWGTGFSMIAEAYVN
                     GGYIGGIVYIFIIGIVIGKILSNSIDNQDIENHPMKMFLAITTANIIMGFPRAASYLI
                     VKNFVYGVLIILLINFFLQQFNKRL"
     gene            606..>1843
                     /locus_tag="ACHA5T_002844"
     CDS             606..>1843
                     /locus_tag="ACHA5T_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFM8888352.1"
                     /translation="MERTARLKKNTIWALVFQITSIVCGMILLRLILTKYGSEVNGMI
                     SSINQFLQIISFLQLGVGAVMQSTLYKPLAEKDNLMISQILASGGKFYRQIAYILLVY
                     VGLLLAVYYVLVPNEFGRSYTMAMIATLSINSFAQYYFGIVNTLLLSADQKIYITYIA
                     QTIAFISNTVACALLISLNTEILMVKLITAIIYLACPIYYRWYVDRNYNIKRNVSYDK
                     EPIPQKWNGVAQHVASVVLDSTDVVVLSIFSNLIYVSIYNVYNLVIYGVKQLFMAMTN
                     GVQALLGELWVRKEIEVLYKTFAWFEWIVHTLTVFIFGCTGILIVPFIQVYTRGVTDA
                     NYNQLSFAALLVTANALHCLRTPYHVMIKAAGKYKETQNSFIIASLLNLGLSLILVKP
                     LGLVGVALGSLISITYHTLW"
BASE COUNT          586 a          258 c          327 g          672 t
ORIGIN      
        1 gtggaggact aagtaaactt actttaaaag attttacatc tgtatttgat tcagatgatt
       61 ctcctttagt tgattctttt tttgaaatgg gggggactat gagtattata acttttttcc
      121 taataagtgg taatggaata tatccctttg ctaatactta tttgacctca ctcttaggct
      181 ctatatcaac aagaatgcta tcaatgtttg gaataaagtt tgttttatta gctgattggt
      241 tttctcagga ttatttagga atcacttggg ggactggatt ctcaatgatt gcagaagcct
      301 atgtcaatgg tggatacatt gggggtatag tctatatttt tattattggt atagtcatcg
      361 gtaaaatcct ttctaatagt atagacaacc aagatattga aaatcatcct atgaagatgt
      421 ttttggctat aactacagca aatattataa tgggttttcc tagagctgct tcatatttaa
      481 ttgtgaaaaa ttttgtgtat ggtgttttaa ttatattgtt gataaacttt ttcctacagc
      541 aatttaataa acgtctttaa tatttaagct taataaaaag ataaaacaag tcaggagttg
      601 caaaaatgga aagaactgct cggctcaaaa aaaacactat ttgggctctg gtgtttcaaa
      661 taacttctat tgtctgtggt atgatcttgc tacgtttgat attaacaaag tacggatccg
      721 aggttaatgg aatgattagc tctattaacc aatttctaca aattatttct tttttacaat
      781 taggagtagg cgctgtaatg caatcaactc tatacaaacc actagcagaa aaggataatt
      841 taatgataag tcaaatctta gcttctggag ggaagtttta tcggcaaatt gcatatattc
      901 tactagtata cgtgggtttg ttacttgcag tctattatgt tctcgttcct aatgaatttg
      961 ggagatcata tacaatggca atgattgcaa cattaagtat taattcattt gctcaatatt
     1021 attttggtat tgttaatact ttgttattaa gtgctgacca gaaaatttat attacgtata
     1081 ttgcacagac aatagcattt atttctaata cagttgcatg tgctttatta attagtctga
     1141 acacagagat ccttatggta aaacttatta ctgctataat ttatcttgct tgtccaatat
     1201 attatagatg gtatgtggat cgaaactata atataaagag gaatgtgtca tatgataagg
     1261 aaccgatccc tcaaaaatgg aatggggttg cacaacacgt agcttctgtt gtactagata
     1321 gtactgatgt tgtagtgtta tctatatttt ctaacctgat atatgtatct atatataatg
     1381 tgtacaattt agtaatatac ggagtaaaac agctattcat ggctatgaca aatggcgttc
     1441 aggccttact aggggaactt tgggtcagaa aagaaataga agttctttac aaaacatttg
     1501 catggttcga atggattgta catactttga cagtttttat ttttggctgt accggtatac
     1561 taatcgttcc atttattcaa gtatatacaa gaggagtaac agatgctaat tataatcaat
     1621 tatcattcgc agctttactt gtaactgcta acgcacttca ctgtctgcgt acaccttatc
     1681 atgtaatgat aaaagcagct ggtaaatata aagaaactca aaatagtttt ataattgctt
     1741 ctttgttaaa tttaggttta tcattaattt tggttaaacc actagggcta gttggagtag
     1801 cattaggatc tctaatttca atcacatatc atacactttg gag
//
LOCUS       DAYYGO010000221         1742 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000221 DAYYGO010000000
VERSION     DAYYGO010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.231"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1598
                     /locus_tag="ACHA5T_002845"
     CDS             <1..1598
                     /locus_tag="ACHA5T_002845"
                     /inference="COORDINATES: protein motif:HMM:NF019348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="McrB family protein"
                     /protein_id="HFM8888353.1"
                     /translation="IPVFSINNSDSVIKDETSFENQLLNGKLLGKIINFSKDNEDYPL
                     NVFWENTDGSRVLFGEIIGQNNSMYGVAYRIEKDNFYKKFIDEGSKNYVGYDDFINQT
                     KDIIFVPYLLLQKIIETKKDINEGIENISDSKISPVSSIITEQTQIDISELEDKHAIQ
                     QNDHFSEHNEFIERFNQLTIRSKLHYSKRDLWNFHTAMLGDSLVVLAGLSGTGKSQLV
                     TSYAKALQLSDTQIKFISVRPFWEDDSDLLGYADTVNSVYRPGDSGLIDALLEASRHK
                     ENIYMVCFDEMNLARVEHYFSQFLSVLEMEPNTRKIKLYNEDLENKLYNSSTYPSTIE
                     VGQNILFVGTINTDESTHQFSDKVLDRSNIISLELVPFHEISEVNDYIQSDQKKRNQL
                     KYDDFKNFKNSNPTNGLTQKEKEMFWKLHLAINSKDKNIGIGWRILKQIDEYLKNLPV
                     QNDLSRKEAIDIQLVQRVLSKIRGSDEQLSELVGKWNENDLKQVGEFEQIINEYPESS
                     DFKYTKKILLQKARELNLHGFTL"
BASE COUNT          684 a          233 c          278 g          547 t
ORIGIN      
        1 ctataccggt cttttctata aataacagcg attctgtaat caaagacgag acaagttttg
       61 aaaaccaatt actaaacggc aaactactcg gaaaaattat aaatttctca aaagacaatg
      121 aagattaccc tttaaatgtt ttttgggaga acactgatgg atcaagagtt ttatttggtg
      181 aaataatcgg acaaaacaat tcaatgtacg gtgtagcata taggatagaa aaagataatt
      241 tttacaaaaa gtttattgat gaaggtagta aaaactatgt aggttacgat gattttataa
      301 atcaaacaaa agatataatt tttgtgccct atctattact tcaaaaaatt atagaaacta
      361 aaaaagatat taacgaaggc atagaaaata tctctgattc caaaatatct ccagtaagtt
      421 caattattac tgaacaaacg caaatagata tatctgagtt agaagataaa cacgctattc
      481 aacaaaatga tcacttctct gaacataatg aatttataga aagatttaat cagctcacaa
      541 ttcgtagtaa attacattac tcaaaacgag acttatggaa ttttcatact gctatgttgg
      601 gtgatagttt ggtggtatta gctggactaa gtggtaccgg taaatcacaa ttagtaacat
      661 cctatgcaaa agcacttcaa ttatcagata cacaaataaa gtttatttct gtgagaccct
      721 tttgggagga tgattctgat ttattgggct atgctgatac tgtaaattct gtctatcgtc
      781 caggagatag cggtctaatt gatgcactat tagaagcatc aagacataaa gaaaatattt
      841 atatggtttg ttttgatgag atgaatttag ctcgggttga acattatttc tcccaatttc
      901 tttcagtttt agaaatggag ccaaatacta ggaaaatcaa actgtataac gaagatctag
      961 aaaataaatt atataactct tcaacctatc cttctacaat tgaagtagga caaaacatat
     1021 tatttgtagg aacaataaat acagatgaat ctactcatca attctcagat aaagttttgg
     1081 atagatcaaa cattatttca cttgagttag taccattcca tgaaattagt gaagtaaatg
     1141 actatattca atcagatcaa aagaaacgta atcaacttaa atatgatgat ttcaaaaatt
     1201 ttaaaaactc taaccctact aatggtttga cccagaaaga aaaagaaatg ttttggaaac
     1261 tccatttagc aattaattct aaagacaaaa atatcggtat cggttggaga atattaaaac
     1321 aaattgatga atatttaaaa aatcttccag tgcaaaatga cttatcaaga aaagaagcta
     1381 ttgatattca gcttgtacag agagtattaa gtaaaatcag aggttctgat gaacaattat
     1441 cagaattagt tggtaaatgg aatgagaatg atttaaaaca agtgggtgaa tttgaacaaa
     1501 taattaatga atatcctgag tcttcagatt ttaaatatac taaaaaaatt cttttgcaaa
     1561 aagctaggga gttgaattta catggattta ccctttagtc tgaaattaaa ctttggttat
     1621 aaaagtgaag aacaagaata tgaactttta ttttttcaag atgaggcatc gttattagag
     1681 tatacaccgg ttaaggtaaa agaattttct aatctaagct ttacatttaa cagtatagac
     1741 aa
//
LOCUS       DAYYGO010000222         1735 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000222 DAYYGO010000000
VERSION     DAYYGO010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.232"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..182)
                     /locus_tag="ACHA5T_002846"
     CDS             complement(<1..182)
                     /locus_tag="ACHA5T_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8888354.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="ACHA5T_002847"
     CDS             517..951
                     /locus_tag="ACHA5T_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8888355.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="ACHA5T_002848"
     CDS             974..1294
                     /locus_tag="ACHA5T_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888356.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="ACHA5T_002849"
     CDS             1435..1626
                     /locus_tag="ACHA5T_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888357.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
BASE COUNT          658 a          260 c          363 g          454 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataat
//
LOCUS       DAYYGO010000223         1733 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000223 DAYYGO010000000
VERSION     DAYYGO010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.233"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1118
                     /locus_tag="ACHA5T_002850"
     CDS             <1..1118
                     /locus_tag="ACHA5T_002850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8888358.1"
                     /translation="SGEYIIDKEVGNMRKLTFVIGIMGKGGAERVIANLANNFIQDNW
                     EIDIITIYGNRQDYELNRKIQVHPIICKSSFRAFRPLERFKELRKKIREIQPDLVISF
                     LADVNIHVIISLLGVKQKLIVSERNDPQRNPEEPWIRKIRDFLYKNVDGIVFQTPDAK
                     KYFSKINDKKVKTIIIPNPIKENLPYYNKTTSSKKFITACRLDKQKNLPLMIDAIGKL
                     IHEGYECELDIYGEGGLKTELESYINHNGLTEFVHLKEFTNKIHEKMQNAAGFVISSD
                     YEGISNSMLEALAIGVPVIATDCPVGGARMYLGDRNEIGHLVECNNLDKLTDAMRRVL
                     LDRESVVRKAQKGIKLRKELTSKNISNIWYEYLNQFF"
     gene            1137..>1733
                     /gene="wzy"
                     /locus_tag="ACHA5T_002851"
     CDS             1137..>1733
                     /gene="wzy"
                     /locus_tag="ACHA5T_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy"
                     /protein_id="HFM8888359.1"
                     /translation="MKEKINILMFSLTLVAFTIFCAFSLNQNYWLNWLGILIYLYAIC
                     TWHWRKGDSIFSLYTIFFTLFLLFSYGQCIMWAFGIGTDRGIGTTVVAYGTGIIPSES
                     DMIMVKWYSCISMFVFHIGALLFTRKTTLRKVSWYESGDADADRKFLFKIGCIISIIV
                     VPIVFVSKLLEVRIALVHGYNALYYGDYATQSGYLQIIL"
BASE COUNT          640 a          200 c          338 g          555 t
ORIGIN      
        1 gctctggaga atatataatc gataaagagg taggaaatat gaggaaattg acctttgtga
       61 taggaattat gggaaaagga ggggcagaac gagtaattgc gaatttggca aataatttta
      121 ttcaagataa ctgggaaatt gatattataa ctatatatgg gaatcggcaa gactatgaac
      181 tgaatagaaa aatacaggta catccaataa tatgtaaaag ttcgtttcgt gcatttagac
      241 ctttggaacg ctttaaggaa cttaggaaaa aaataaggga aatacagcct gatctggtta
      301 tttcgttcct tgctgatgta aatatccatg tcattatttc attgttagga gtaaaacaaa
      361 aattaattgt ttctgaacga aatgatccac aacggaatcc agaagaacca tggattagga
      421 aaattagaga ttttctttat aaaaatgtag atggaattgt ttttcaaaca ccagacgcaa
      481 aaaaatattt tagtaagatc aatgataaaa aagtaaaaac aattataatt cccaacccaa
      541 taaaggaaaa tttaccttat tataataaaa ctacaagtag taaaaaattt attactgcat
      601 gtagattgga taaacaaaaa aatttgccct taatgattga tgcaatagga aaacttattc
      661 atgaaggata tgagtgtgaa ctggatattt atggagaagg gggattaaaa accgagcttg
      721 aaagttatat caaccataat ggattaacag aatttgttca tctaaaagaa tttacaaata
      781 aaatacatga aaagatgcaa aatgcggcag gatttgtaat ttcttctgat tatgaaggta
      841 tatctaattc tatgttagaa gcactggcta ttggagtgcc agttatcgct acagattgtc
      901 cagtaggtgg cgcaagaatg tatttaggtg atagaaatga aataggtcat cttgttgaat
      961 gtaataatct agataagtta acagatgcaa tgcgtagggt acttttggat agagaaagtg
     1021 tggtaagaaa agcgcaaaaa ggaattaaac taagaaaaga actgacatct aaaaatattt
     1081 caaatatatg gtacgaatat ttaaaccagt tcttttaacc aaaaggagga tattgtgtga
     1141 aagaaaaaat aaatatattg atgttttcgt taacgttagt cgcgtttaca atattttgcg
     1201 ctttttcatt aaatcagaac tattggctaa attggcttgg aatattgatt tacttatatg
     1261 caatctgtac atggcattgg agaaaaggag attcaatatt ttcattatac acgatttttt
     1321 tcactctttt tttattattt agttatgggc aatgcattat gtgggcattc ggtattggaa
     1381 cagatcgtgg aatcggtaca acagtagtag cttatggtac ggggattatt cctagtgaat
     1441 cagatatgat aatggtaaaa tggtattctt gtatttctat gtttgtattt cacataggag
     1501 cactcttatt tacgagaaaa accacactaa gaaaagttag ttggtatgaa tctggagatg
     1561 ctgatgccga tagaaagttt ttatttaaaa tagggtgtat catttctata atagtagtac
     1621 cgatagtttt tgtttctaaa ttactagagg tacgtatagc attagtccat ggttataatg
     1681 cattatatta tggtgattat gctacacaaa gtggatattt acaaattatt cta
//
LOCUS       DAYYGO010000224         1726 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000224 DAYYGO010000000
VERSION     DAYYGO010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.234"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..186)
                     /locus_tag="ACHA5T_002852"
     CDS             complement(<1..186)
                     /locus_tag="ACHA5T_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888360.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHR"
     gene            complement(188..1615)
                     /locus_tag="ACHA5T_002853"
     CDS             complement(188..1615)
                     /locus_tag="ACHA5T_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFM8888361.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKEN"
     gene            complement(1608..>1726)
                     /locus_tag="ACHA5T_002854"
     CDS             complement(1608..>1726)
                     /locus_tag="ACHA5T_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HFM8888362.1"
                     /translation="DQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
BASE COUNT          671 a          291 c          190 g          574 t
ORIGIN      
        1 ccggtgattc acaaaaacaa catcattaaa tagccctaat tcttttatac gctcactata
       61 tcttctagcg ttttcaaatt cagaataaat aaataaatct ccttttgatt ctcggtaaaa
      121 attttgataa acatttaata tattaatcaa atgtaaagga gtccaagcaa tgaaagctgt
      181 cttcatatta attctccttt tttaaataca agtaagaaat gcttcccaaa taataccaga
      241 aaacaattga aatgacatcg cgcttattga aaagtacatg agcctccacc atttcatcta
      301 taaaaataga tagtataaaa attaaaacaa taccaagaaa cgcataattc actttacttc
      361 taaaaaatgt tttgagtaaa ctaaagatat ttaacacata gaaagcatag atacatataa
      421 aagcaattat accacttgat acaagtactt gaagaaatcc attatgcatg tttccatgtg
      481 ctctttttag ttcattcaca tctgtagtat caagatctac ttttttcaaa aattttgcat
      541 cgctattccc atagaaatcg gtctctccat aaccaaataa caaagttggt cttataactt
      601 taagcgcagc tttccaaatc gtaaaacgac cagctgaaat ttcggagtta tcactagaat
      661 gctgtaatat tacttctttt tctggaagtg gagttttatc tgattcattt gactctataa
      721 cttctacttt tctatcaatc gatacaggaa ttgttgctaa aaaatcctta gcataattag
      781 taccaaaaga aaaatatacc aatagaacta ataatatgct taccatccat ttgattttta
      841 caggtagtac ttctctaatg ctctttctaa attttgaatt ggcaaacaac aaaagaaata
      901 atactaaaaa agaacaaatt actacctggg cacctctaga cgaagaaagt gtaatataaa
      961 atatttgtac gataaaattc atcatataaa aaacacgaat cgtttttgtt atttttgaaa
     1021 ttaatacact aattaaactt aaaataatag aagtatatcc aaacatagct ccaacatttg
     1081 gacttgtata gacaccaaat aaccgatgtt caataaaacc ttgtcttgca agtaatcctg
     1141 gcgttcctgt tgggacatga taagaaatca aaaaaataaa ttgatagata gatattagcg
     1201 cagctaaaaa aattatgata atgaaaacat tattaaaata caccattttt ttcttttttt
     1261 cctcttcact ttgtgaaaag tctgcaggaa aaattactat caaagtaatc agcaaatatc
     1321 caaaattata aatagatgga attaaaaaat tttctctgtt aatgattata gttaagataa
     1381 aactaatcat tgctccaatc agtagccaag aaaaacgcat tttaaaaaaa tttttcttta
     1441 taaaaacctc atacaaaact ataattccac cccaagcaaa agctattttc atcgggatat
     1501 tagttacatt aaaagtacta ggcataaaat aaagcagaca aatgattaaa tttattactc
     1561 gaaacgcatc tcgacttaat aaactcttct ctaaaacagg tactattcta ttcatctata
     1621 tttccaactt tcaaaatttc tttatcaatt ctatttaata gtttatcaga gtaatccgat
     1681 ttaacttttc ttacaaataa tttttcactt tcttttattt gatcta
//
LOCUS       DAYYGO010000225         1685 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000225 DAYYGO010000000
VERSION     DAYYGO010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.235"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(98..1576)
                     /locus_tag="ACHA5T_002855"
     CDS             complement(98..1576)
                     /locus_tag="ACHA5T_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8888363.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT          537 a          304 c          231 g          613 t
ORIGIN      
        1 atatctagtt atatctagtt atatctagtt accttttctt tgaaaaaaac ataaatcagc
       61 taataaatat gtgagacaaa gctgccatcc ataaatacta gctgattttt tcagttaaat
      121 gtctattttt ttctctgatg atggtatttc caaaatattc ccagtcatct ggagtcggtg
      181 gatttttcca aattatctgt tctttattaa atttttcaag taaacaatca gataaataaa
      241 tattcgtacg agaagtgatt cgactctcaa tcacaatatt gtaattttta aatcctttca
      301 cttgagaaat aatgtaatcg gtataagcag atccttgcga aaaatcaata cagacatgaa
      361 tcggtgcttc aaaatatgat accggacaaa taccgaccaa taaaaagaga taatcataaa
      421 ataagcgaat catgaaacgt tcatcttttc ccaagaaata gcgtttattc tcttgtatga
      481 ccttttggat tgctgtactg aatattggat acgtctcgaa gaaaaactgt agctgctttt
      541 tagacgtaaa gctagtcatc tcaaagtcga atatccaatt ttttcgattg acatgcatca
      601 tacccatacg aagttctttt cgtatatata gtctttcttc ttcagagatt tcaatcgaaa
      661 atggtaattg atcaatcaat tccactgaca ttttttgact aatcatgtgg atatcatgaa
      721 aatgagcttc cgcaagtcca aatgcgccat ctaattcaaa ttcttcttcc gtgatcaaaa
      781 acaacatgat ataagaaatc tcatcaatga tctgctcttt agggatatga agaaaagaaa
      841 tatgtgataa atccttgatg atcttcattc cttctgagct cttaaagtat tcatctgaaa
      901 agaaacgttt tccgattggt tgattcccat aggccctcaa caaactaata gtggttagca
      961 aattgaattt gattcgtttt gttgcagaca gttttaaatt gaacagatat tcaaaatatt
     1021 gcacgatact ctttgcttca ttcatgacgc tttctctaaa tggcatagaa aaaccattaa
     1081 agaccccgaa atatacccca aataatacgt tccgtatgtt tatttcttcc ccaactaata
     1141 tgtttttctt taactttatt tgatgctgtt caagaaaaga gactaattct cttcttttta
     1201 cataggctct tgatatgctt aaaaaatgat tctctgcaaa tttttcgatc gttacttttg
     1261 aatccacgat ctccgttaac agtcgagcaa tttttgatcg ttgaaagtag attcttctta
     1321 aatttttgat atccgtcaaa ttcagacgtt cgacatggat ctctgcttct tctgaaattt
     1381 ggatctctgg ctttgttttg aattcagtaa attctttatt taattctatg atataatttc
     1441 ttgttttaaa tttagacaaa cctaatgttt catataactt aggcagcact acataattct
     1501 ttttctcaaa ttctaaatat ctagctagca gcagtttttc tctatcttcc tcttctataa
     1561 ggtaatccaa cttcatatcc tacacctcaa aaaatgcctc ataccaatta cttgatatgc
     1621 agatgaaagt tcatcttttc acctatactg attttatgat ggattatgct tgttttttca
     1681 tcagt
//
LOCUS       DAYYGO010000226         1680 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000226 DAYYGO010000000
VERSION     DAYYGO010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.236"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..972
                     /locus_tag="ACHA5T_002856"
     CDS             64..972
                     /locus_tag="ACHA5T_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFM8888364.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FERWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            972..1592
                     /locus_tag="ACHA5T_002857"
     CDS             972..1592
                     /locus_tag="ACHA5T_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888365.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
BASE COUNT          602 a          246 c          377 g          455 t
ORIGIN      
        1 cagtattata gtgcaagtgc aatttcttcc attgtcatac caccaaaata gggaggtgat
       61 ttaatggcaa agtggaatgt catattaagt acaacagaac cctataatta cgtggggatg
      121 attcaagttc gacaaggcaa taagaataca gaggttatgg aagcgactat agttgaaaat
      181 ggtcttccct acgatttatc agaatgtaag gtatattttg aatcagttgt aggtgggaaa
      241 tatccagtcc aattagaaac aaaaattgtg gatgctaaaa aagggaaaat taactatatt
      301 tttgataaat attccatgca gtgtttacat cgacaaacag ccaatttcat tatatttaaa
      361 ggagaagact tgattggaac aactcaagac ttctcttatt ttgtcattaa tgctgtttca
      421 aaaacagaag gagaaatggg ttcttattgg caatcaatcg aagatttaat tgcggatatg
      481 acggacttta ttaatgaaaa taaaggcgat tttacggact ggatgaatga aaggaaagaa
      541 gaatttgaac gttggagaca agaacaggaa cacagtttcc aagattggag agaaggacaa
      601 gaatcagact atttggcatg gtttgaatca atcaaggata ttttaaagac gattgatcca
      661 ggtggagtca tgttagccga actaacggat gcgcgagtag atattcaagg agttcgtcat
      721 gcgtctattt cagagcgttt gctggcagat atggactatt tatatcagaa attgcgagca
      781 acgcttttca cgattgaata cggggagatt gaagtgactg atattttgca ggatgatctc
      841 ttttcagata atcatgaagt cgaaaaagtt ggaactgtag cattcccgat tgaagaagga
      901 gccttgatca tcgcaaccgt tgatgatcca aagcaaaatg tgttcactct tgagaaagtt
      961 ggggtgatct aatggctaaa actaaacgaa tgatggaaac ggatgaaaaa acgggtgtac
     1021 aacgccaatt ctttccaatc acacatgctt ccgcagttct tgggttagaa gaaataatag
     1081 caggagaagc aacagtttta tctgttaatg gaaaaattgg agccgtcgtc attactaaag
     1141 aagatttggg attagagaat gttctcacag aattacccta tgcaagtgaa gaagatgacg
     1201 gcattatcac ggcagaaatg tatcaaaaaa ttttaaacag tggagaaggt gactacgtgt
     1261 tgccagttgc cactatcgac cgtttgggtg gcataaaagt tggtgaatta ttgacgattg
     1321 atgagacagg gaaagtctct gcagtcagac aatctgatgt caatttttcg ctggagttaa
     1381 aagaaaaact cgattcactg aaaaattata ctgctggaga aaacatcact attgatgaag
     1441 atggaacaat tagttctaca ggtggtagtg ggactggtgg tgtcaatcag tcatatgtag
     1501 atcaaaaatt ccaagaagct gtaaatcaag cagaaaatta cacaaacgaa agaattccaa
     1561 attttacttt tgaaaaaatt ggggaggtat agagaatgac agatatcgta aaaataaaac
     1621 aaagcaatgt acaggtttat ccacaaactc attggaatgc tatagaaggc aaaccaacaa
//
LOCUS       DAYYGO010000227         1677 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000227 DAYYGO010000000
VERSION     DAYYGO010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.237"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..972
                     /locus_tag="ACHA5T_002858"
     CDS             64..972
                     /locus_tag="ACHA5T_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFM8888366.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            972..1589
                     /locus_tag="ACHA5T_002859"
     CDS             972..1589
                     /locus_tag="ACHA5T_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888367.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
BASE COUNT          620 a          226 c          348 g          483 t
ORIGIN      
        1 cagtattata gtgcaagtgc aatttcttcc attgtcatac caccaaaata gggaggtgat
       61 ttaatggcaa agtggaatgt catattaagt acaacagaac cctataatta cgtggggatg
      121 attcaagttc gacaaggcaa taaaaacact gaagttatgg aagctacaat tgtcgaaaat
      181 ggtcttccat atgatttatc agaatgtaag gtatattttg aatcagttgt tggcggaaaa
      241 tatccagtcc aattagaaac aaaaattgtg gatgctaaaa aagggaaaat caaatatatt
      301 tttgataaat attccatgca gtgtttgcat cgacaaacag ccaatttcat tatatttaaa
      361 ggagaagact tgattggaac aactcaagac ttctcttatt ttgtcatcaa tgctgtttca
      421 aaaacagaag gagaaatggg ttcttattgg caatcaattg aagatttaat tgctgatatg
      481 acagatttta ttaatgaaaa taaaggcgat tttacggact ggatgaatga aaggaaagaa
      541 gaatttgatc gctggagaga ggaacaggag aatagtttcc aagattggag agaaggacaa
      601 gaatcagatt acttgtcatg gtttgaatca atcaaggata ttttgaagtc tatcgatcca
      661 ggtggagtaa tgttagccga attaatggat gcacgtgtag atcttcaagg tgtacgccat
      721 gaatcgattt ctgaacgttt cctatccgat ctaaattatt tgtatcaaaa aatgaaagca
      781 gcacttttca cgattgaata cggtgaaatt gaagtgactg atattcttca agacgatctc
      841 ttttcagata atcacgaagt cgaaaaaatt gaaacagtag aatttccaat tgaagaagga
      901 gctttgatta ttgcaactgt tgatgatcca aaacagaatg ttttcactct tgagaaagtt
      961 ggggtgattt gatggttaaa gtaaagagaa tgatggaaac tgatgaatct ggtgttaaac
     1021 gtcaatttat gccaattaca catgtctctg cagttttagg cttagaaaaa atgatatcag
     1081 gacaatcaaa agttctttcg gttaatggta aatatggtgc agtcatttta acgaaagctg
     1141 atttaggatt agagaatgca attactgaac taccttatgc aagcgagaca agcgatggta
     1201 ttcttactgc tgaaatgttt caaaagatcg taaatggaga aggtggaaca tatattttgc
     1261 cgattgctac tcctgaacaa ttaggaggta taaaagttgg ggaactctta gaagttactg
     1321 aagaaggtgt tttatctgca actaagcaaa cagacttcaa tttctctgaa gaattaaaag
     1381 gaaaattaga atctctaaaa atattaaaag caggtgcaaa tatatcaatt gcagaagatg
     1441 gaacaattag ttctacaggt ggtagtggga ctggtggtgt taatcagtca tatgtagatc
     1501 aaaaattcca agaagctgta aatcaagcag aaaattacac aaacgaaaga attccaaatt
     1561 ttacttttga aaaaattggg gaggtataga gaatgacaga tatcgtaaaa ataaaacaaa
     1621 gcaatgtaca ggtttatcca caaactcatt ggaatgctat agaaggcaaa ccaacaa
//
LOCUS       DAYYGO010000228         1673 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000228 DAYYGO010000000
VERSION     DAYYGO010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.238"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(540..1565)
                     /locus_tag="ACHA5T_002860"
     CDS             complement(540..1565)
                     /locus_tag="ACHA5T_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8888368.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
BASE COUNT          521 a          267 c          322 g          563 t
ORIGIN      
        1 tctaaaataa tttgtctaac tagaagaaaa gtgggaaagg atcttaacaa catcggttcg
       61 tatccaatta aggtatcgga acgatcatct tccaagagaa gtcaatcaac aaaagtaaag
      121 atagatcatt ttttgaatgc aaaataaatt gatgggaaga tagagcggtt atttttaata
      181 ttttgctagc tattattgaa taattcctcg aataaaacaa aaccagtaga catgtctgtt
      241 attctattta tgttttagag ataagtatcc ccaaataaca tggattgaaa taagggtaaa
      301 tgtgtttaaa aaaacgaact ataaaaactt atacacaacg tggttaaaaa tagttttttc
      361 ttccctgtgg aaaaaatagt ttaacacaaa cattatagtt atccacagct atctaaaaca
      421 gatggaaaat aagaaaaatt cgctaaaata ccgggagggg gtaacgaggg atgagtgatt
      481 gaaaaaagaa cagagtaact gacttatcct cagtgtttct gttcttttat aattctgttc
      541 tacatttctt gttgttggat cgaatctgtg agagtaatct cggttgaaag aatgattctt
      601 ttttcttcag ggtattggtg gtggattagt tggaataaaa gttctgtggc tttgattcct
      661 aactgatttg tcggaatgtc tatccgagtc aactgtggcg aaaataggga ggacaataag
      721 ggatcactga atcctgcgat cgaaagatca tcaggtatct gctttccaga ttgttctgcc
      781 actttgtaaa tactcaatga ttttaatgca tcggtagaaa aaatcgcagt tggtctttct
      841 cgtagcttca ttaaatcatt cattgctgcc atgctatctt ctaagcgaaa gccgccgtct
      901 ataatccatt cattacggat atctaggtgg tgatcaaata agcattgacg gtaaccattg
      961 agacgatcat tggatacata aacttctgtc ggagcatgga gacagccgat atgacggtgt
     1021 ccattcttga tcagtgtttc caccaaatcg taactatcct ggaaattatc cgtgtccact
     1081 gagtagatgt tcgtgtagtc gtttccgact tttccgatta ccacaatagg aatattgtat
     1141 tgatccaatt caaaaagaag tgtttcatcc atcgtagagc ttaaaataat aattcctcgg
     1201 atcatttttt cttggatctt tttcgcgcat ttttctagct cttcttctgg tgtttttgcc
     1261 gtttgtagaa tcaagtcgaa gttattttgt tcagaaacgg cagaaatgga ttgaataaca
     1321 tctgaaaaaa aaggattgcc ataggtcgta tgagaagtac gatgagataa tgctagaatc
     1381 gcgtcaaaac catttcctga cagtcctctt gcaattttgc tcggttcata attcaattct
     1441 ttgattgctt gaagtacact tgcttttgct ttatcagaga tatttggctg gttatttaat
     1501 actctggaga cagttgattt tgagacatta gcttttttcg caatgtcata aatagtaggt
     1561 ttcaatttta tatgcacctc ctttttggga gcgttttaga ataattccat agtacaatag
     1621 acattctttt ttgtctatga ttttgaaaat ttaggaatag cttgcatgat cat
//
LOCUS       DAYYGO010000229         1667 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000229 DAYYGO010000000
VERSION     DAYYGO010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.239"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..291
                     /locus_tag="ACHA5T_002861"
     CDS             <1..291
                     /locus_tag="ACHA5T_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFM8888369.1"
                     /translation="EIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFPM
                     KMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            284..580
                     /locus_tag="ACHA5T_002862"
     CDS             284..580
                     /locus_tag="ACHA5T_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888370.1"
                     /translation="MAKLIVLGGLIYGGYWLYQYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHTGYRHYWERID"
     gene            655..1653
                     /locus_tag="ACHA5T_002863"
     CDS             655..1653
                     /locus_tag="ACHA5T_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFM8888371.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKATIETDTTIETKKSKTQS
                     TEQTTTEMKQSVETSASSITESSSEEVKNTLWDTNKASKLETFVTQWGKTLGQEYKSY
                     NLQNNVSLYGTPLPQAVINGDWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVETGE
                     YLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFSDIVNGQTPQTP
                     VVEESTQQATTTTTQSTSSSGISSWEAAKESVLNNKALWLYDSPDKNVSNTNLISTNE
                     YMKDLMQDENGSYYSINIVTDDDGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK"
BASE COUNT          641 a          230 c          327 g          469 t
ORIGIN      
        1 gagattgatg aaacttgttt gagtaactct tataaaagta tgaatgactt ttttgaggaa
       61 atggaactgg attcagaatt gctctatcaa ttttatcttg cttttcgagg gcaacagatt
      121 tcttttccaa tgaaaatgta cgatcgtgag ttagttagaa aaagaatcga aaacatgata
      181 gaacaggaaa aaactgtaga tatccgtcaa ttgacagaag tttatggttt ctctgcaaga
      241 tggattcaaa aagtaattaa acaaaaagaa aggagacgag tacatggcta agctgattgt
      301 attaggtggt ttaatttacg gtggatattg gttatatcaa tatatgcttg cgcatccgaa
      361 tatagataaa ggaaaagtaa ttaagacagt tggggtaatc atagttggac tcatagctct
      421 aactaagaaa aataatacta atagcccaaa aataatgaga acttatcgct gtagaggatg
      481 tggaagggaa atttctacta gttttaatcc atctgagagt cctcaaggaa gagaccgctg
      541 ccatacgggc tatcgacatt attgggaaag aatcgattaa aagattacca tttagatcaa
      601 ttaattgtct gatagaataa gtacgtgagg gagaaattga aagggggaaa tataatgaaa
      661 aaatggctgt tattactaat aggaatggga attttattca ctggttgtac atctgagaat
      721 acgaggacta aagcgactat agaaactgat acaacaattg agactaaaaa aagtaaaacg
      781 caatcaactg aacaaactac aacagaaatg aagcaatctg tggaaacaag tgcaagttca
      841 atcactgaat cttcttctga agaggttaag aatactttgt gggatacgaa taaagcttcc
      901 aaattagaaa cttttgtcac acaatgggga aagacattag gtcaagaata taaaagctac
      961 aatcttcaga ataatgtaag tctgtatggc actcctttac cacaagccgt gattaatggc
     1021 gattggaaaa tggcaatcaa tgaagcacca gttaccgtac aatggtcaga agatggaact
     1081 ggtcatgcag atttcaattt agtagcagtt tattctgatg tagaaactgg agaatattta
     1141 gggcagcatg tttacttttt cggttttcaa aatggacaac ctaaagttta tttaacgcaa
     1201 caaaatcaag gtaatgaaaa taattatctg tactttaacg aaactcaaaa tcaacagtta
     1261 aaacaaggtt tcagtgatat tgtgaatgga caaactcctc aaacaccagt agtagaagaa
     1321 tctactcaac aagcaacaac tacaacgaca caatctacaa gtagttcagg aatttcaagt
     1381 tgggaagctg ctaaagaatc tgtgctaaat aataaagctt tatggttgta tgattctcct
     1441 gataaaaatg tatcaaatac taatttaatt agcacaaatg aatatatgaa agatctaatg
     1501 caagatgaaa atggaagcta ctattcaatt aatattgtaa cggatgatga tggaaacacg
     1561 accatggctg gtggaaaaca atttcgtgtt tatacagatg gaagaatttc tcaacgtatt
     1621 ggaatgacta acaattttca aatgattaaa taattgaaaa tattatt
//
LOCUS       DAYYGO010000230         1664 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000230 DAYYGO010000000
VERSION     DAYYGO010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.240"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..505
                     /locus_tag="ACHA5T_002864"
     CDS             131..505
                     /locus_tag="ACHA5T_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM8888372.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACHA5T_002865"
     CDS             complement(619..1248)
                     /locus_tag="ACHA5T_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8888373.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ACHA5T_002866"
     tRNA            complement(1382..1454)
                     /locus_tag="ACHA5T_002866"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          283 g          557 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DAYYGO010000231         1641 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000231 DAYYGO010000000
VERSION     DAYYGO010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.242"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            128..1594
                     /locus_tag="ACHA5T_002867"
     CDS             128..1594
                     /locus_tag="ACHA5T_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFM8888374.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          460 a          296 c          337 g          548 t
ORIGIN      
        1 attgccatta aaaattactt gcttttatag aaaattagtt tatatttctt aaataagttt
       61 tttagttaaa tttttcggta aagatgtata atattttctg aaaatttaaa gaattggggt
      121 atagattatg gaaaaacagc aagacaaggg cttcttcggg cagccgaagg gattgtcaac
      181 tttgttcttc actgagatgt gggaacgatt tagttattac ggtatgcgtg cattacttat
      241 ctattatatg tacgatacag ttgccaacgg gggacttgga ttaccacgag caacagcatt
      301 agctatcatg tcgatctacg gatctttgat ctacatgtct agtatcgtcg gcggatgggt
      361 atctgaccga gtgttaggag caaaacgcac tgtattcttc ggtggagttt tcattatgct
      421 tggccacatc gtcttggctt taccatttgg tgtaagtaca ttgtttgttt cgatggcatt
      481 gattattatc ggaaccgcct ttttgaaacc aaatgtttct ggcatggtcg gacatcttta
      541 ttcaaagact gacttgcgaa gagatgcagg attttcgatt ttttacatgg gtgtcaactt
      601 aggggcatta ttagcaccac taatcgttgg tacaatcggt caagaatata actatcattt
      661 agggttctct ttagctgcta ttgggatgtt ctttggatta ttacaataca tcatccaagg
      721 acgcaagaca ttagacggag tgggtacaac accgccgaat cctttgacaa ctgaagaacg
      781 taaaggattc atccgtaact tgatcatcgc acttgttgtt atcgctgcag tattcggcgg
      841 ggcacaacta actggtcatc tgacaattga cttttttgtc aatacaatca gtgtattggg
      901 gattatctta ccgttgtatt actttatcaa aatgctgact tcaaaagatg taactgctga
      961 agaaaaacca agagtatggg cttatatccc attattcctt gcagcaatca ttttctggtc
     1021 attacaagaa caaggttcat ctatcttagc tttatttgct agtgaacgta cacaatctac
     1081 gctatttggt gtaacgatcc aagcaagctg gtatcaatca ttgaatccaa ttttcatctt
     1141 tattctgaca ccagtattcg taacgatgtg gacgaaactt ggtgaacgtc aaccatctac
     1201 agtagtgaaa ttctctcttg gacttttatt tgcaggatta tcgtacttgc tattgatgtt
     1261 gccgggtatg ctttatggta caagtggaaa agtaagtcca ttgtggttgt tgggaagttt
     1321 cttcatcgtg gttattgctg aattgtgtct ttcaccagtt ggtttgtctg tgacaacaaa
     1381 attagcacca cgagcatttg aatcacaaac aatcgctatt tggttcttag ctgatgcttc
     1441 ttcacaagca atcaatgcac aaattgcacg cttctacaca ccgggaacag aatctgctta
     1501 cttcggtatt atcggtgcag tagcaatcgt cggtggtatc ttgctattta ctgtgaaaga
     1561 accaatcaaa aaactaatgg gctctattca ttaatcaatg aacataaaaa caggaattgc
     1621 ttatttgcag cagttcctgt t
//
LOCUS       DAYYGO010000232         1640 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000232 DAYYGO010000000
VERSION     DAYYGO010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.243"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            416..>1640
                     /gene="ltrA"
                     /locus_tag="ACHA5T_002868"
     CDS             416..>1640
                     /gene="ltrA"
                     /locus_tag="ACHA5T_002868"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFM8888375.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRL"
BASE COUNT          586 a          299 c          388 g          367 t
ORIGIN      
        1 aacttggtgg tgaaagtcca ctacaggctt ggcagtagga actgttagcg aaagacaagg
       61 cggcaatcgt gaggttgtgg ctgaaggaag tcggacgcaa actcctgaac tgacgaacag
      121 aaactagata caaggctgaa ttaggtcgga taagtcttca tcacaagaca aagtccaata
      181 ctgcccgaaa cctatacagt aaatctagca gttacatggg aggaaagttt tcactcttac
      241 cgggggagat ctcatgaggg tgttgtgggc gttgaatgaa aacaaaccag cacaacgatc
      301 atggcgacat gatggtgaat catgagaagt cagccgaggt catagtagta acgacactga
      361 ccgttacgaa ggactgaaca ataagaatct tgaataattt aggaggtgtg agaagatgcg
      421 acgaccgcag aaaacaagaa aacttggcta tcatcaaaga gctagtgtgg aacacgaagg
      481 gtatgatgaa gtgcgcagca cttcttgcgg tgaacagagg aaacaagatg gtgtagaaaa
      541 tctgtttggg agaattctag atcggaataa tctcaatcaa gcgtatcttc aagtagtcag
      601 aaataaaggg gcggctggtg cagatgggat gaccgtcgat caactgcttc cttacttgaa
      661 agagcaccgc gaggaattac tatccaattt acgccaaggg aactataaac ctcagcctgt
      721 tttgcgagta gaaataccga aaccaaacgg cggtgtaaga aaactaggaa ttcccacggt
      781 tattgatcgt atgcttcaac aggcaatcaa tcaagtgtta caaccgctat ttgaaccaga
      841 attctcagat aatagttatg ggtttcgccc aaatcgcagc gcacatgacg cagtgaagaa
      901 agcaaaatct tattatgaac aaggttaccg atatgtggtg gacattgata tgaaagccta
      961 ctttgacacc gtaaatcacg ataagctgat gtatttcatt gagcggaaag taaaggacaa
     1021 acaagtccta aaactgatcc gtcaatactt aatgagtggt gtaatggaaa atggactcat
     1081 cacaacaact gaagaaggaa cgccccaagg aggcaatctc tctcccctgt taagtaacat
     1141 ctacctgaac gaatttgatc aagtgctaga aaatctagga cataaatttg ttcgatacgc
     1201 cgatgactgc aatatctatg ttagaagcaa acgagcagga cagcgtgtga tgaagaaatc
     1261 cattcaattt ctcgaagaac aactaaagtt gacggtaaat cgagaaaaga gtgcggtagg
     1321 aagtccattg aaacgaaagt ttcttggatt ctgcattctg ccaacaaaga aaggtattaa
     1381 aattcgccct cacgcaaaag caaaacagcg tgtgaaaagc aagctcaaac aactcactaa
     1441 aagaaaccgt ggaagaagta tccaacttat tttaaaagaa atcaagcaac taatgactgg
     1501 ttggatcaac tactatggaa ttggtgagat gaaagaattc atgagggagt taaacggttg
     1561 gttgaagcga agaatcagac aatatatttg gaaacaatgg aagaatccaa gaaccaaaag
     1621 aaaaaatctg attcgtttag
//
LOCUS       DAYYGO010000233         1632 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000233 DAYYGO010000000
VERSION     DAYYGO010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.244"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(69..446)
                     /locus_tag="ACHA5T_002869"
     CDS             complement(69..446)
                     /locus_tag="ACHA5T_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888376.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(746..1627)
                     /gene="galU"
                     /locus_tag="ACHA5T_002870"
     CDS             complement(746..1627)
                     /gene="galU"
                     /locus_tag="ACHA5T_002870"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFM8888377.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          449 a          365 c          242 g          576 t
ORIGIN      
        1 ttaaataaat ctctcatctc aatagtttat ccattatttt atcttttttt gcgtatacct
       61 actaaatatt accgtcttct tctcgttctt ctactttgtt cgagagaatc tgtactagaa
      121 aaagtagttg tatgtctacc ttttctgcct atccgagact ttcttttcat atatgggata
      181 taaatgatta gatacagtac agcaaaaaat aaagcaaata cgcaagcttc ccaactacct
      241 aacgccaatt caaccatcaa ggtagcaacc ccaacggatg ctgcatacca gataaaaatc
      301 agaaacgttt ctttttttct gaccatgcga aacatgataa atagactaat aagcaatagt
      361 ggcaacacaa atatccaatt gcttccttcc atcagccact tgagtatatc aagcagcgac
      421 tgtgcaacag cgactaattt tcccatagtg aacacacacc tttttttata attaaaaaat
      481 tcgccttttt attctactat gaatcttctg gcttggcttt catttctctt atgtaatcat
      541 tttcttttat acaacttaaa aaaaaattaa accattagca taatattttc tgaatctatt
      601 cacgaaagtc tttttctatc tttcgttttc cacgactcaa ctgatctaga gtcaaaaaac
      661 taaaacgaca gttgcgcaaa tgacgcacaa gctgccgctt taataacaaa tagcctttca
      721 ttctcagcag gccacttatt tccagttaac tttctgttct tagcttttta ctcaactgga
      781 taatatatgc acttaatgaa gtcccaattt ccgaatgttg aagtccatat tcaatatttg
      841 tcttcatata tcctaactta tcgcctacgt cgtatctttt tcctttaaac tctctcgcat
      901 agaccgcctc tgtctggtta agagtctgaa gtgcatccgt tagctgtatc tcgtttcctg
      961 ctcctggttc ttgagtttct aatatctcaa atattttagg agtcagtaga taacgtccga
     1021 taatggctaa attacttggt gcttttccca tttcaggttt ttctacaaac ccttttactc
     1081 tgcaacgacc cttttcgcta tctgttttag gatcaataat tccgtattta gatgtttcat
     1141 tttctggtac acgcatgacg gcaacagcgg aagcttgtgt ttcttcataa tccatcatca
     1201 attgttgagt caatgggata tcatctttca tcaaatcatc ccccagcatg acgacaaacg
     1261 gttcatttcc tataaatgct tttgcttgaa gcacggcatc ccccagtcct cttggatgag
     1321 attgacggat gaagtgaaga ttgacttgag tcgtttcttc aactaaagcg agtaattctg
     1381 ttttttcctt ttcccgcaaa ttgttttcca attcgatatt tgcatcaaag tgatcttcaa
     1441 tgggtctttt ttcttttccc gttacgatca agatgtcttc gattcccgaa tccaatgctt
     1501 cttccacaat aaactggatc gttggtttat ccacgattgg aagcatttct tttgccatgg
     1561 cttttgttgc cggcaaaaaa cgtgttccta gcccagcggc aggaatgact gcttttttga
     1621 ccttcacttg ct
//
LOCUS       DAYYGO010000234         1549 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000234 DAYYGO010000000
VERSION     DAYYGO010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.245"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(136..426)
                     /locus_tag="ACHA5T_002871"
     CDS             complement(136..426)
                     /locus_tag="ACHA5T_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888378.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(544..804)
                     /locus_tag="ACHA5T_002872"
     CDS             complement(544..804)
                     /locus_tag="ACHA5T_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888379.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(941..1354)
                     /locus_tag="ACHA5T_002873"
     CDS             complement(941..1354)
                     /locus_tag="ACHA5T_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFM8888380.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
BASE COUNT          581 a          247 c          229 g          492 t
ORIGIN      
        1 tgttactatg aataacataa taagaaagta aagtggattg atttgagatc tttttatact
       61 tcaaaaaaga taacacccaa ctttcaaaaa tatgttttgg aagttgggac aaacgagtta
      121 atacatttta atacattatt ttttattcaa aaaaaatagc acaatggcaa agatcgtaag
      181 agagatcata agccccaatg aatgactaaa aagaaaagca gaatcacatg ccatatcatc
      241 ccatatttct tcaagaaaat aaataagcgg gaaaaaggga aggaatagta gaacatcgat
      301 ccagcaccat ttttttcttc ttttgaaata acataaaaca ataaacatga acgtgcaggg
      361 aagtaaaaac aaagctttac tcataactaa caaaatacca gctcctttta tttttttctc
      421 ccccacattc aaagagtaac aatggtatgt acttaataat agaaaaatgg atcaacttgt
      481 atgaataaca gctgaaaaga tctgcaccaa ataaaaaact tccagccaaa actgactgga
      541 agtttatttt tttctgaaat attctaacac aaatatgaaa aagatggccg aagctaataa
      601 gccgatttcg tagcaaaaca atgagatacc agagtcgtaa taataaaatc ctctaatctt
      661 atggataaaa tagatcatcg gacaagcaga taagagtaag tatttgttag gccttttgaa
      721 taagtaaaag aaagcactgg tcattatgat aaatggtatc caaaacaaaa gtttacttaa
      781 gacaatcaaa tgatcaactc ccatattttt taccaagaga gatcataaca tctttatcgt
      841 aaaaacaaaa tatttttttg ctcattttga aaatttaaac atactcgttc ttatccaaat
      901 atataaaaat cgcccctcat tcatcgagag gcgataagtt ctaatttaac tcaatttttt
      961 ttgtttcttt tggctgataa gctgttttag gtaataccaa tttcagcaca ccgtttttaa
     1021 aggttccagt gattttttct tcatcaacat taggaagata gaatgaacgt ttgtaagaga
     1081 ccgaatgtct ttctttccgt acatagttgc catcttcttt atcttctgta tgggactcat
     1141 gattggcgga gatcgtcaaa gtattatctg aatattcaac tgtcgtgtct tctttgtcac
     1201 agccaggcat atcagcagtt agtttgtatt ctttttcatt ttcgactaaa tctacttttg
     1261 gataacttga aacacctaaa aaatcattga atgctggact aaacacgtca ttgaaatcag
     1321 ggaacatgtc gcgaatgcta ggtacatttg ccatttttga tcatcctttc aataagtatt
     1381 ttttatttat ccctatttac gtaataactc tattttgaaa aaaatgcaag tataatggtt
     1441 agctttgtga atatttcatc taatacgata caaaggctat ctttctaatt tgaaaaaggg
     1501 atcattaaaa taataagaag aaaatatgtc aaaaggtaaa aaagtaaat
//
LOCUS       DAYYGO010000235         1538 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000235 DAYYGO010000000
VERSION     DAYYGO010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.246"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            35..577
                     /locus_tag="ACHA5T_002874"
     CDS             35..577
                     /locus_tag="ACHA5T_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888381.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            733..1182
                     /locus_tag="ACHA5T_002875"
     CDS             733..1182
                     /locus_tag="ACHA5T_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFM8888382.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDGMATLFLTKIIRCCECCGVMATIALDNEHPRI"
     gene            complement(1212..>1538)
                     /locus_tag="ACHA5T_002876"
     CDS             complement(1212..>1538)
                     /locus_tag="ACHA5T_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8888383.1"
                     /translation="GKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVE
                     FVHQYQFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSD
                     TSGEAA"
BASE COUNT          583 a          218 c          302 g          435 t
ORIGIN      
        1 atatttcatc aacgtttcat aaaaggtgtg tagaatgtta ttgaaagtag ctataaaatg
       61 ctattgtgta aaaaataaag gaggaataat ttatgaaaaa atagtaccaa gaggagcatg
      121 cctattactt gcattatgtt tgagcttaag cgcagttggg tgtcaaaaaa gtaatgaaaa
      181 caataaaata aaagaaggac aatccataag ttctgaagag gaaggcatgt caattaatgc
      241 aaatggcaaa aatgaaactt ttaagccatc agattataca ttggaggcaa agaaagaata
      301 tgtttatgaa tatttagggc ttaaatttaa actctcagac aaatttagaa attatatagc
      361 tgataaaaag atagcgatgt tagatgatca aagtcctata gataaagaac taaagtatgc
      421 tattttgaca tttgagaaaa tgacagaaga acaaaagaat gcagttatag aaaagatggg
      481 cgatggatat aaaaattggc aaaatgagct tgaaagaatt gggactattg gtatatttga
      541 aaaaaataca tcagaagaaa aaaatctaaa attataaaat gtgatactca tactaaaata
      601 ggagtttcaa gtgatggaaa atacgattgc tatttaagca caaatagcgg agctgaaagt
      661 aatcttctgg atgaattaaa aagaactgag attcaaatta tagataaaaa agaacgtcct
      721 aaaaatggtt ttgtgctatc agaaaaaact gatttagaaa atacagaagc atttaacaaa
      781 gaatcggtta aagatttacg taaactacca acaaaggaca taaatggcaa ggattttaca
      841 agtaaagatt ttgagaagta tgatcttact atggtaaatg tatttgcaac ttggtgtaca
      901 gcttgtgtaa aggaaattcc tgatttggtt gaggttcaga atgaaatgaa aagcaagggt
      961 gtaaatatcg ttggagttgt tacagatcct gttgatgata acggtgaaaa caaggaagca
     1021 attgaaaagt caaagttaat acatgaaaag acaaaggctt catatccttt cttaatgcca
     1081 gatggaatgg caacactttt tttgacaaaa attataaggt gttgtgaatg ctgcggtgtt
     1141 atggctacga ttgcccttga caatgagcat cctcggatct gattttctcc ggcagaagca
     1201 caatcattca attacgctgc ctctccagag gtatcagatc cgcgctcatt atcaaggatt
     1261 cgcttcgcca atctctgttg tcgaatttgg atcggtgttt catacgccaa cgtgccatgt
     1321 aagcgtagat agttccacca atgcacataa tcaaacaatt ctagacgtag ctgtgccagt
     1381 gtctcaaatt ggtattgatg cacgaattct actttgacag atttataggt tgattccaca
     1441 acggcattat catacggaca acccttccta ctcaatgaac gagtaattcc aaaaccattc
     1501 agaatctcat gaatggtttg gttatcaaat tcctttcc
//
LOCUS       DAYYGO010000236         1510 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000236 DAYYGO010000000
VERSION     DAYYGO010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.247"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            66..1235
                     /locus_tag="ACHA5T_002877"
     CDS             66..1235
                     /locus_tag="ACHA5T_002877"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM8888384.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
BASE COUNT          541 a          272 c          243 g          454 t
ORIGIN      
        1 tcaagataca agctatttta gctttattct ttctgtaaaa aagaaaggag actaaataaa
       61 ctattatgaa aaaaggaaaa catgctaaaa aaagaaaata ttcattttta atgatcgttg
      121 tcgtgttgtt tagccttagt gcgttctcta caggaactat tcttggatgg caattttaca
      181 ataatgaaca ctctcagata gttgcccatg ctcaatcagc tcaaaaaaag ttgatactga
      241 ctaccaatga acaacagctc gttgaaaaaa tcgatcaact attaagaaaa aatgattata
      301 ttggctcaat ttacgtaaag aaaaataatc gcatcatcat ggaaaaaggc tatggctatg
      361 cgaataaact cacgcaacaa ccaaatgatc cttctcttta ttatcagatt ggttcggttc
      421 aaaaagcatt gactactctt ttgattttaa aacaagtcga gcgtgggcac ctttctttag
      481 atactaagct ggctcaattc tatccacaaa ttgctggcag ccagcaaatt actatccgtg
      541 acttgctgta tatgcgctca ggcttgcacc gaactgcttc tccaaccata ccgatgactg
      601 atgaagaagt cgttcaattt gcgattcagc acttaaaatt tacagattat cagacctctc
      661 attatgattc attgaacttt accatattga ctggaatttt gatcaaatta acaaatcaac
      721 cgtatgaaaa attattgacc gatgatatca tcgaaccttt aggattacag catacaaaat
      781 tttatgacca agtcaaaaat tcatccaatc atgcactatc ctatcaaatg tctaaagagg
      841 atgattactt taaaccactg acagaatctg aaacagagat ccataacgaa ttagggactg
      901 gtaatatcag tatgtccgtc tatgatttaa atacgctgtt tacacaagtt ttatctggaa
      961 aactagtttc caaagactta ctcttctcat tatggcgtga aaatgcgaat ggacatccat
     1021 atagtggtgg ggtatatagc ggagataatt atatcctgtc gcaaggaaat atcgactctt
     1081 ttgatacatc tgctatattt agcaaagatt cacaggatgc tatcatcatg gaaagcaata
     1141 taaaagctga caaaactatc aaactgccta ctgctgatct aagaaaccaa atctatgacc
     1201 tgatggaagg aactactaag ttggcaaata gctgatatga tgaaaagata gcaaatacta
     1261 cttctttgaa tagcatttgc ttaaaaaaag tactcgacta aacgtaggaa agagtgtggg
     1321 acaaatctaa aaatcaattt ttgctacaga ctttaattcc aaataaacag aacagaaaga
     1381 ggtgttcttc tttctgttct gtccaaatta ttctaagatt tctctttacc aacattattt
     1441 gacaaagggc ctattaaatc taaaatagta tctttagatt gctaaaatat gtgctagcaa
     1501 atcattcatg
//
LOCUS       DAYYGO010000237         1507 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000237 DAYYGO010000000
VERSION     DAYYGO010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.248"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            173..>1507
                     /locus_tag="ACHA5T_002878"
     CDS             join(173..614,614..>1507)
                     /locus_tag="ACHA5T_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFM8888385.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSI"
BASE COUNT          618 a          200 c          259 g          430 t
ORIGIN      
        1 aattcacatg attatcgaat tttattaagt tagtttttct tgtaaatctc tgttcccaat
       61 gaaactcgag acacttattt taaagaggaa acaatgtggt atttaaatag ccctcttgtt
      121 gatgtgcacc ccaaaagtta gactagaaat ctaattaaaa ggggtgcttt ttttgcggaa
      181 atatacattt gtttttaaga aaaaagtagt ttcagactat ttaaataacg aaggcggcta
      241 caaatatctt gcacataaat atcaaataaa tcgtacactg gttagacatt gggtaaggat
      301 ttataactat catggttggg aaggcttggt tggaggtggc aaaagctaca ctacaaaatt
      361 taaacttgat gttatagaat atatggaaac aaatggtctt tctatccaag aaactgctaa
      421 aaaatttaat atcggttcaa atagaactct aagtaaatgg atagagcaat atgaagaagg
      481 cggtgcttct tcacttgaga gccaaaaaag gggcagaaaa attagtatga attccaagct
      541 aaacattcct aaaaaactta aagatgagtc tcttgaagaa gaagttattc gtttaagagc
      601 agagaacgca tatttaaaaa agttagaaac cttgattcaa gaacaagatt tgagcaagaa
      661 gaaatcaagg ttaaaacaat ccttcagctc aaaaaagaat ttaaattgaa tctattactt
      721 tcaattgcac aattagcgaa gtcaacttat tattactggg taaaaaaatt agataagcca
      781 gataaatata gcaagataaa gcaagaaatt acagcgattg taaaagaatc aagaaactct
      841 tatggttatc gtagagtcac tttagcgtta aagatgaaag gatatacaat caatcataaa
      901 acagttagaa aattaatgtc ccaaatggga cttacctgtc aaatccgaat aaagcgatat
      961 aaatcttata aaggaacagt aggaaaaatt gccaagaatg tgttaaaacg aaatttttca
     1021 gtagatacac ccaataaaaa atgggtgaca gacgtcactg aattcaagat aaaaggaaga
     1081 aaaatctatc tatctcctat acttgattta tttaacggag aaataattag ttatagtata
     1141 tcaactagtc caacatataa actgattgaa gaaatgcttc aacaagcaat taaaaaaaag
     1201 ggaactgagg gttcactgat tctacattca gatcaaggat ggcagtatca gatgccacaa
     1261 tatcaaaaaa aattaaaaga gaataacatt attcaaagca tgtctagaaa aggaaattgc
     1321 ttagataatt ctgtaataga aaattttttt ggtgtgctta aatcagagtt tttttatcga
     1381 gaaaaattcc gatcaattga gatatttcaa agtaaattaa atgagtatat taggtggtat
     1441 aataacaaaa ggataaaact aaagttaaat ggactatctc cagtagaata tcggaaacag
     1501 tccatta
//
LOCUS       DAYYGO010000238         1504 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000238 DAYYGO010000000
VERSION     DAYYGO010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.249"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(280..353)
                     /locus_tag="ACHA5T_002879"
     tRNA            complement(280..353)
                     /locus_tag="ACHA5T_002879"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(317..319),aa:Ile,seq:cat)
     gene            complement(542..>1504)
                     /locus_tag="ACHA5T_002880"
     CDS             complement(542..>1504)
                     /locus_tag="ACHA5T_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM8888386.1"
                     /translation="PINGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRN
                     EAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKA
                     TFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSE
                     MGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIE
                     NANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRADVNQAYNTSGNVGYHVS
                     FNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          442 a          298 c          270 g          494 t
ORIGIN      
        1 tttaaagcgt aacacactta tggggcagtg gtttttgggg aacgctttaa actcttcttt
       61 attattatct cacaatataa cccaaatgtc tttctattta aaaatcaaag taaaactttt
      121 caaatataca gaaatataac tatgtgaaac atcctttcat taatccataa aaggatacat
      181 aaaaaagcca ctcatttgag tggcaatgaa gaaaagcttt agcttgtata atactcttca
      241 aaaaattcta acacagaacg attcaaatgg ctacgttaat gtaccctgta ggactcgaac
      301 ctacgaccgg acggttatga gccgtctgct ctgaccaact gagctaaggg tactggttgt
      361 tgccacataa agccataaac aatcaaccag tagaatgtgt ggcaacaaac ctgttatcgc
      421 atagcttgga gtgtgactat tatgggtgat agtgaagata tgcgataaca tcactatttt
      481 atcgaatgat ttttatagtt gtcaatatag ttatgtaatg ctcctcaacg aggagctatt
      541 tttatcgttt aggaatattt aaataccaac gtttgtcatg gaaatcttgc gcaccgcctt
      601 tagtgtttcc ctctggatca ttcgttgccc gcatcatgac atagactttc ttattaggga
      661 agttacgcat gttaaaagat acatgatagc caacatttcc agaagtatta taagcttgat
      721 ttacatctgc tctataaatt ccatcagctc ttactcgagc taattctttc ccagtattgt
      781 aatccataat gaagatatac tcgtatttat agttagcaat gtgccatcca gctacatgca
      841 aatttgcatt ttcgatttct ccaaactgat caatgtgggc gtaattcgtt ccatctgtca
      901 gtgtaggatt agctgcacca gctcgtgttg gatcaatgac aggcttgttt tcagaagttg
      961 ttggattttc atcggtaaat ccatgagcta aatcatatgc taatttttct ttacttacgc
     1021 ccatttcaga aagataaccg taaggatctg tatgatcgcc ccagatgttt tgtgttaccc
     1081 ataaatgcga tttgattccc ggttggttat aaggagtgtc caacgttaat ggaataccat
     1141 atttcattgc tgaatctcta gccaattcaa cgtatgcctt gtagtttttc tcaaacgttg
     1201 ctttatcatg tgtgtgttgt aactcaatct gcacaggact gttggcatta gcatacgaac
     1261 cagcaccgta ctgtacataa ccaggttgac cgacttgata aacaattccg ccgtctccca
     1321 caatataagc agtataagcg ctagtccatg aacgttgcat atactgcgct tcattgcgtc
     1381 ctgttgctgt ttcattagcc gtttcatgca gtaaaatata ctgattattt gctacttgtg
     1441 agctaccttc atttgcgccc aaattaaatt cattgttgat agtataggca aatccgttaa
     1501 ttgg
//
LOCUS       DAYYGO010000239         1499 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000239 DAYYGO010000000
VERSION     DAYYGO010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1499
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.250"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1486)
                     /locus_tag="ACHA5T_002881"
     CDS             complement(<1..1486)
                     /locus_tag="ACHA5T_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888387.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSFYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLE"
BASE COUNT          466 a          290 c          178 g          565 t
ORIGIN      
        1 tttccagaag tggaaggata tcttcattca atttttgaat cacagaagtt gcattaaaaa
       61 cgtctaatcg atagacattg cttctcttat gattaaagtt tcctaacgct ttctcttcct
      121 ttaacctatt taggtattcg agcatcttca aaggaacatc ttcatcatct aattttttta
      181 tcaatcctat aaaagtcata agtgatagta acttattaca tttatctatg tcataagttt
      241 taccatagta aaaactcatt tttttagcta attgttcccc cgacaaaata caagtcacac
      301 tttccttact atcatcaaaa aaattaacat cagaaagcaa gacatcatag acacgtcgca
      361 tttcctctag ataattcctt attgtcctga aaagtttgtc tttcattgat tcaagagtgt
      421 caaaaagtac ttttgattcc tctttcaatt ttcgaaaatg tgtaggatac aagtctaggt
      481 tctctaatag ccaattaaat gtttctaccc gactgtcttt ctttaaaatt agcatcacaa
      541 ctccaataaa attttcactg atatcacaat tactggaatg acaataaaaa atggaatgcc
      601 cagttttact ggtaaaaata ctagcagagg gattatgatc gttgtgaaac agacatctta
      661 agtttttact tgacttaagt aatttattca tatcaatggt taacaatctt tcataaatct
      721 cattaaaaga catgtcggag gaatcaaaca ggcagtcttt aaaccactgt ttcatttctt
      781 ctttgtcgtc attttttatc aacttaacaa aatcggtttc attgactagt tcatactctc
      841 tccctgaatc actttgacaa tttttagaac atgtcatggt aaaatcaatc ggaagctcag
      901 taatatctaa catgttgtct tcattaatta ttatcactgc atctgactta tatcctccaa
      961 aaaacattct attcgaagca tttacattgt catcacaccc ggggatgttc tttttcaagt
     1021 attcgtaaac agcaaccatt ttaccatagt cagtaattgc ttcttttaat agaaagacaa
     1081 tcttgtattt ttctagattt tcagagtacc gaatactttt ttgtatcgca aacgcagaat
     1141 tatgaactaa attgatagat aaaacctttt ctatgcttaa gtatctacta tcatctttac
     1201 tcaaatctac taccttcccc ttctcatctt taacagtgtt atcgacatcc agaataatta
     1261 ctctggtttc cacaaaactc tccttgtcat tgcctttatt ctttgtcttg cttggaataa
     1321 atgattgtga tgaatttagt atctctacaa actcatctct tgtaacttct ctatagttat
     1381 ctaaacacct atctcttaca tcagtttttt ctttttttgt aactggcttc tctaataatt
     1441 tttctttatc tacataaatt ggaatattaa tcattgatgt ttccatttgt gtttcctcc
//
LOCUS       DAYYGO010000240         1462 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000240 DAYYGO010000000
VERSION     DAYYGO010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.251"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            77..487
                     /locus_tag="ACHA5T_002882"
     CDS             77..487
                     /locus_tag="ACHA5T_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8888388.1"
                     /translation="MIKVYTSPSCTSCRKARAWLKANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            501..857
                     /locus_tag="ACHA5T_002883"
     CDS             501..857
                     /locus_tag="ACHA5T_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8888389.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          540 a          209 c          271 g          442 t
ORIGIN      
        1 tattttatta gctaataatt ggtattttaa aagaaattac tgtagaattg gattataatt
       61 aaaaaggagt gagaaaatga taaaagtata tacttcgcct agctgtactt cctgtagaaa
      121 agcaagagca tggttgaaag cgaatcaact tgaatttgaa gagaagaata tttttgccga
      181 accgttaaca gaaagtgaga tcaaaaagat cttggcagcg actgaaggag gagttcaaga
      241 tattatttcc actcggtcga aaatttacga aaagctgggc atagatttta acgaattaac
      301 tttaaagcaa atgatcgtct tattcaaaga atatccttca ttattgagac gacctatttt
      361 gattgatgaa aaaagaatac taatcggatt taatgaagat gagatccggt catttgtccc
      421 tcgtgaaatt cgatgtgttg ccagaaatct gatctattta caaaacaata acatcttaaa
      481 ttcataggga gggagccttc gtggaagtga aagtctatgg atcagcgcac tgtgtaacaa
      541 gtctgaaggc gaaacaatgg ttagaacacc atcaattgcc ttatcaattt gttgacttag
      601 aagaacgcga tttaagtaca gaagaagcag aagaattatg cagttttgaa gaagtgcaag
      661 cagaacagct atttgcgact tggtctgagg cttttcacaa cattgcgatt gacctttcct
      721 gtgtaggaaa aaaacaactg atttttttat gcacacaaca taccaatctc ctcagacgtc
      781 ctttgattat tatcgatgaa gttctattta ttggatacaa tgagcagttg atggaacagc
      841 ttatcttaaa taaatagact ttaactgttt taatcaagtc actgagcatt tcatacagtt
      901 gtaacagtcg aacacttagc atcaagcgtg ttatgtgttc tttttttgac tttatttttc
      961 tactatagag acatgctcaa tttatgtggt caaaattcaa atctaggtgg ggatatagag
     1021 ttaaaaatat attgacatgt gtaaatttac atgatatatt catttctgtt gttttaagta
     1081 gcaaagcaat aaaatagtat tttaaaaaac atataaaata gttgttgaca tgttagaaat
     1141 gacatgataa gataactgag ttgttaaaaa agaaccactt gtgaagcttg attctatcga
     1201 aaaaaacttc aaaaaagttc ttgacaaata acaaatgata agttatgata aatgagttgc
     1261 tgtaagattt cgaaaaacga aatcaaaaaa acttttaaaa aagagttgac aattacttgt
     1321 ctctctgata tgatataaaa gttgctacgg cagccaaaaa cgaatcgggg aacactcctg
     1381 atgaagtaga cctttgaaaa ctgaacaaag taagacaaac caaatgtgta gggcgtcttg
     1441 attcaattca agacaacaaa ca
//
LOCUS       DAYYGO010000241         1431 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000241 DAYYGO010000000
VERSION     DAYYGO010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.252"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            173..1405
                     /locus_tag="ACHA5T_002884"
     CDS             173..1405
                     /locus_tag="ACHA5T_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFM8888390.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
BASE COUNT          470 a          268 c          309 g          384 t
ORIGIN      
        1 ttagtttttg attttcttca caagctgaaa tagcaagtag taacatatat catgaaactt
       61 aaccttaaaa aaataacctc ctttttttat ttaaagcctc attgtttgaa tcataattaa
      121 atggtatcat gctaatagtt accagaaaaa atggtgagga ggaattttta ttatgttatc
      181 tgattttaat gaacttctaa aaaaatatgc acgcttaatt gcagaaactg gcgtagccac
      241 agaaaaagga cacacagttg ttttacagat cagcgtggaa caggcacccc ttgctcgatt
      301 gatcacacaa gaagcttata aactaggagc cgctgaggtt atcgttcaat ggactgacga
      361 ccagatccaa agagaatttc ttttacatgc tgcaacggat cgtattgaga atgtaccaca
      421 atctaaaatc gatcaagcgg atgaatggct tgaaaaagga gcaagcagga tcagcgttgt
      481 ttctgccgat cccgatgcat ttgctggtgt agatagccat cgagtagcgt cttatcaatc
      541 agcgaatggc aaagcactaa tgaatttacg caaggcaact caagcaaata aagtcagttg
      601 gactgtcgtg gctgcggctg gtaaacaatg ggcagcaaaa gtcttcccag aactgccaga
      661 agaaaaacaa gttgaggcat tatgggatca gattttcaaa acgactcgtg tctatgaaga
      721 agaccctgtc ttagcatgga agaagcatga tgaaaaatta gcaaaaaaag cagaagaatt
      781 gaatcgagaa caattttctg cccttcacta tactgctccc ggtacagata tcattatcgg
      841 attgcctaaa aaccatcttt gggaaggcgc tggaagctat aatgctcgtg gagaaaaatt
      901 catggcaaat atgccgacgg aagaagtgtt cacagcacct gacagtcatc gagtagatgg
      961 ttacatttca agtacaaagc cactgagtta tgctggcacc atcatctcgg gtatgaaatt
     1021 tacttttaaa gatggaaaag ttgttgattt ttctgccgaa caaggagaag atgtcttagc
     1081 aaaactgctt gatacagatg aaggtgcgcg tcgtttagga gaagtcgcac ttgtacctga
     1141 cccttcacct atttctcaat ctggcatcat ctttttcaat acattatttg atgaaaacgc
     1201 atcgaatcac ttagcactag gttctgctta tgccttcagt gtgaaaggcg gtacagaaat
     1261 gtccgatgaa gaattagcag aagctggatt gaatagaagt caaacccacg tagatttcat
     1321 ggtaggatct gacaagatgg atatcgacgg aatccgtgaa gatggctcta cagtaccgat
     1381 tttccgaaat ggcgattggg cttgagtata ctttcgcaca catgcaaaaa a
//
LOCUS       DAYYGO010000242         1428 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000242 DAYYGO010000000
VERSION     DAYYGO010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.253"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1087
                     /locus_tag="ACHA5T_002885"
     CDS             <1..1087
                     /locus_tag="ACHA5T_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8888391.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1294..>1428)
                     /locus_tag="ACHA5T_002886"
     CDS             complement(1294..>1428)
                     /locus_tag="ACHA5T_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFM8888392.1"
                     /translation="HNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI
                     "
BASE COUNT          506 a          251 c          269 g          402 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaaccac cggtggcaaa tccatgtaaa catggtttat
     1141 tttttacact gttttcaaac aaacccttgc tatttcttcg gttcgtcctc gtccattttc
     1201 gataccgaca gctagcttat aacccaaggt atatcaacat aaaaaaataa aaataggcta
     1261 tgacagcagc gtgacagcaa aaaagatgga aagttaaata tcaaacctcg taatttcatt
     1321 tgcgagtgct tgatttgttt ctggatataa gtgtccatat ttgtccaaag tagttttaat
     1381 agaggcatga cctagacgct tagatattgc atagatattt ttattatg
//
LOCUS       DAYYGO010000243         1413 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000243 DAYYGO010000000
VERSION     DAYYGO010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1413
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.254"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..965)
                     /locus_tag="ACHA5T_002887"
     CDS             complement(<1..965)
                     /locus_tag="ACHA5T_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HFM8888393.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPI"
     gene            complement(970..1155)
                     /locus_tag="ACHA5T_002888"
     CDS             complement(970..1155)
                     /locus_tag="ACHA5T_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM8888394.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
BASE COUNT          435 a          268 c          172 g          538 t
ORIGIN      
        1 tgaattggtt ttactcctgc aatttcagct agttttttta ttctgcttaa aatagttctt
       61 ggagatggcg gtaatccatc aaaagaaaaa accaattcta tatttttccc aattgattgt
      121 tgagtttttt tccatgtttt taaatcttca acggtttgtc tgtctaacga aattatccga
      181 atacttgatg tattttttgt ttcagcaaat tcataatctt gtcttgtacg gatgtaaatt
      241 gacttattta cgctacatct gccatttatt aagtcaaagt ctttccattg taacgctaag
      301 agctcctcac tccttacacc tgtaacaaaa ataaaacgca tcaaacgttt ataaaattct
      361 tcataaaaat ccccgtgata aacactatta tatactttct taaattcatc aactgtccaa
      421 aattctactt gaggacgctg agtttttatt ttacctatag tatctacagg attttcttca
      481 ataatttcaa gtacaattgc ccttttaaaa actactctca acttattgaa tatctgttta
      541 atataatttg gtgataatgg ttgctcaatg ccctttttta caacatggat tttcgataaa
      601 aactgttgaa attcctgtat atgaattggt cgaatcttta caacctgcat ttctccaaaa
      661 aaatccaatg cacgttgtaa aatatgatct gttttcatat atgttttttc tacagttccc
      721 aacttatacc aaggaaaaaa atactctcga taaaaatcat gaaacgttat tccttgcaga
      781 taaattcctt gtgaatcaga aaaactttgt tttaactctt cgagtcccct tctagcttct
      841 ccttgagttt tgaagcctct tctggtcact tgaattcttt ttccaaagcg atcttgtcct
      901 aaagttgcag aaaaaaacca agtgcctctt tttctgtctt tataaacatt cgcaattttc
      961 ctcattttac tattcctccc aagaaatatg gagaacttct tctaaaattg aaataggaat
     1021 tctttttatt tttgaattac gataaaaaaa gtatcctttc tcaactaaaa gttctttaca
     1081 ttccttgaag attttattag catagtactg agaaaaacct agattaatca aatcttttcg
     1141 agtagctgtt ttcattatag caccttcctt actttttttc atttatttct atcagctgct
     1201 tttgtcaaaa gcacatagga atttcacctc tccccaagtt tacggcgagc cttacggaag
     1261 tatcattatc ccaactttaa atcatcgctt tttattgact ataaaaatta ttttagaata
     1321 tttatcgctc cttctttaca gcaagaaatc atggcgcaac taaaatatgc tccttaaagt
     1381 gttaacgtac tgatagaatc tatttaattt tca
//
LOCUS       DAYYGO010000244         1364 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000244 DAYYGO010000000
VERSION     DAYYGO010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.255"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>1364
                     /locus_tag="ACHA5T_002889"
     CDS             82..>1364
                     /locus_tag="ACHA5T_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFM8888395.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          465 a          262 c          257 g          380 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taat
//
LOCUS       DAYYGO010000245         1352 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000245 DAYYGO010000000
VERSION     DAYYGO010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.256"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1352
                     /locus_tag="ACHA5T_002890"
     CDS             <1..>1352
                     /locus_tag="ACHA5T_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888396.1"
                     /translation="FYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALT
                     IIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQ
                     IKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSI
                     IQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLF
                     QKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAA
                     KENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGES
                     WEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDV
                     NAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKK
                     "
BASE COUNT          469 a          200 c          243 g          440 t
ORIGIN      
        1 gtttttatta tggagaaaag ttttttaaaa agatagatgc tgctttttta tttagttgtt
       61 tatatacttt tgcaacttat cgaacagttg atatctatta tcgatccgca attgcagaag
      121 caatcgctct tacgattatc ccagctgttt tatattatgc ttatggattg atattcaata
      181 gagaaaaaaa agtcatcgct ttatctgtca gtataagtct acttgtgtat actcatgtct
      241 taactacact attgtgtagt gcattgattg cttgtatgat tatagttcgt ttgtttttaa
      301 gcaagcaaat aaaaaacgat ataaaatttg tggtcgtgaa tttagtaaag gctgtttttt
      361 tgacttgtgc attaactagc tatttttggt atcctatgtt gcagcagatg aattatcaag
      421 aaatacacag tcccttcaaa acagatttgc agctagaagc actttctatt tcagatagta
      481 tcattcaagc gttaaataac gatattacaa catacactat ggggttatta ggattggttg
      541 cgttgatttt tcctttattc atactgaaag aaatggagaa aaaagaaaaa atcatctatg
      601 ttggcgtagt ttctacatgg gtatgcgtaa ctaatttatt tccatggttt ttgtttcaaa
      661 aaacaccgat aagcttgctc cagtttcctt ggcgtatctt aggtatccaa atcttttttt
      721 cttcaatgat cataacgctt gtttttatga aaaagactat gaataaaaaa tattcatggt
      781 taatcattgg cgctactgtg gtgtttctac tcgtcacgtc tatagcagca aaagaaaatt
      841 acgcaaaagt tatccaatca aaacacggtc atattgttat caatgaagaa acactcccac
      901 gttatacaac atcagatatg ggaggattat acgattacgc cccaacagaa gcgataaaag
      961 aaaggcctca cttagaaaaa catgaagtaa aagtaggaga atcgtgggaa aaagggaagt
     1021 ataaagctgc agataataac attacttaca cagtggcaag caacaaaaag caaaaagtga
     1081 cagtaccagt atatgcttat ttaggtacac aggtaaaagt aaacggtaaa gtcgtaaatg
     1141 actcttataa aaaacatggc ttagtgaatg tggatgtaaa tgctggagaa aataagatag
     1201 aaattacaac aaaatatact ccaactgctc tcatggcttt atggatcagt attctgactt
     1261 attgtttagt ggtgtattca agttttaaac gatttgcacc taataagttg aaagattctc
     1321 gatatgtgac taaaaaggag ataaacaaga ag
//
LOCUS       DAYYGO010000246         1349 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000246 DAYYGO010000000
VERSION     DAYYGO010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.257"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            30..233
                     /locus_tag="ACHA5T_002891"
     CDS             30..233
                     /locus_tag="ACHA5T_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8888397.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            249..587
                     /locus_tag="ACHA5T_002892"
     CDS             249..587
                     /locus_tag="ACHA5T_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFM8888398.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            574..753
                     /locus_tag="ACHA5T_002893"
     CDS             574..753
                     /locus_tag="ACHA5T_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888399.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(796..1266)
                     /locus_tag="ACHA5T_002894"
     CDS             complement(796..1266)
                     /locus_tag="ACHA5T_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888400.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
BASE COUNT          488 a          183 c          263 g          415 t
ORIGIN      
        1 gaggaaattg gttagaaagg agttttagta tgactgacat tgcagaaatc actcaacgag
       61 atagagaaaa aatcaaagaa tatgtcgaaa gttcaaagtt cttaacttac accatgcttg
      121 ctgaaagatt tggaattagc aaaagctact tatctttaat tttaaacggt aaaaagactt
      181 ctgcagaagc aaacagaatt atagattcga ttatcactat gtacgaattg taaagggagg
      241 aacagctaat gcaatatcta gaagcaaaaa tcccaattcc agaaggctat gtaattatct
      301 cccaagtgga ttatgaggag ttaaaaaaag ctgatgatac tggtagatgg atgacgttgc
      361 cagaagtgct agaacggatt aacagaaaat atgattggtt tacttctaga gttttaaaga
      421 acccaagata tagaaatatt atcgatatcg agaaaaacaa aaatggattt gtctattatc
      481 cagttgaagg aagagacaca tatctatttt taagaagtaa aacacttgaa tttttagaaa
      541 caaatttttc ggaaatctta aggaggcaag cggatgggaa aatttaatag agcgctagtg
      601 ttcagcgcac cgctaatcat ctacgcttta ggactttggg gaagcaggca agcgttgata
      661 ggaacgattg tttacatggt ttggattttt atggggcttg atgaagctga agctgagtac
      721 agagcgaaaa agccaaccga gggggctgac taaaaatgaa tagaaaagag aaactagaat
      781 gtatattact attactcagt ttaattcttt cactaatttc tctattggga agtttgtatt
      841 tttgatatca acaattttgt ggatttgggt tctatttaga gtaaatatca tttttgtttg
      901 gtgattaagg agtttattaa aattaatttt ccctacttca aatgcaaggt aacaagatat
      961 agcactttta ggaggaatat ttagcggtac agcttcagag tatattctat tatcatcgac
     1021 tgatcctcct ttagcaatta atttcttaaa tggagtagct gaccatctgc ggtttaagtt
     1081 agcggatgaa agttctaaat taactaacat agctggttct gttgaatagt tagaaatgat
     1141 tacttttgta taaatcatat ctgcagcgaa gtaagaagca ttaagttcaa cttctagttg
     1201 agggcgcatt ccttttattt ttatccctgt taaaaacgta ctcaatataa aacttattat
     1261 tgacatccat tgaaaaattg ttaagtgaaa aaaattcaaa ataaacacca ccagttttta
     1321 actaaattat accaaaaagg agagaagaa
//
LOCUS       DAYYGO010000247         1308 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000247 DAYYGO010000000
VERSION     DAYYGO010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.258"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1308
                     /locus_tag="ACHA5T_002895"
     rRNA            <1..>1308
                     /locus_tag="ACHA5T_002895"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          349 a          287 c          399 g          273 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
      901 atctccccca agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
      961 tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag
     1021 ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt tggaaatttg
     1081 agaggagctg tccttagtac gagaggaccg ggatggactt accgctggtg taccagttgt
     1141 tctgccaagg gcattgctgg gtagctatgt agggaaggga taaacgctga aagcatctaa
     1201 gtgtgaagcc cacctcaaga tgagatttcc catttcttta agaaagtaag atccctgaga
     1261 gatgatcagg tagataggtc aggagtggaa gtacagtgat gtatggag
//
LOCUS       DAYYGO010000248         1240 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000248 DAYYGO010000000
VERSION     DAYYGO010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.259"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(43..1169)
                     /locus_tag="ACHA5T_002896"
     CDS             complement(join(43..941,941..1169))
                     /locus_tag="ACHA5T_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8888401.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAGSHILSENLNSLVQFIEKWCKDYTVS
                     LLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKE
                     GISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIISKENLLNQDFSTETICEKWAAD
                     ITYIPTKKNGWCYLSSIMDLHTKKIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMIL
                     HTDLGSQYTAREVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDF
                     EEANRALFSYIEGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
BASE COUNT          349 a          212 c          201 g          478 t
ORIGIN      
        1 tggatttgag tcaatatctt ggacacattt tagtagagac ggttaagcca ttttttcttt
       61 tgctaattct tcaaattcct gtggggttag atagtgaatc gagctatgga ttcgatttcg
      121 gttataaaat ccttcaatgt agctaaatag cgctcgattt gcttcttcaa aatctgagta
      181 gctagtcgtg tagacttctt cttttttcaa tgaggcatgg aaagattcga ttccagcatt
      241 atcataaggt gttccttttc tactataaga atgccttatt ttgttggttt taagccattg
      301 ttccacttct cttgctgtgt attggctgcc caagtcagtg tgtagaatca ttccttctgg
      361 aatgtgataa tgtatttttg ctttattcaa tgtttgaatg acacaatcca cagtcattcg
      421 ttttgaaaat gtataactaa taattttttt cgtgtgtaaa tccatgattg aagataagta
      481 acaccaacca tttttcttag taggaatgta cgtaatgtca gctgcccatt tctcacagat
      541 cgtttcagta gaaaagtcct gatttaagag attctctttt gaaatgatcg gtttattaga
      601 cctttgaggt ctatattttt taactacaat cgaccttaaa tttaattgtt tcattagctt
      661 ttggacatgt ttaagagata ctgaaatccc ttcttttaat aaaacttgat ggatctttgt
      721 ggcaccatag cgttgtttat tcgtaaaaaa aattgttgaa atcttctttt tcaacttatt
      781 atttctgatt tctgtagctg tcaacggttt attcttataa aaataataga cacttctagg
      841 gatttctaat aaccgacata acaaagaaac agtgtaatcc ttacaccatt tttcaataaa
      901 ttgaacaagc gaatttaggt tttccgagag aatatggctg cggctttttt taagatttca
      961 agttcctcat ttaaacgagc attttctttt ctcagttcag ctacttcttt tccagtcagt
     1021 cctgtggact gatcaggtaa atataaattc ttccatttgt aaatcgttgc ttcagataag
     1081 ccatattctt tcgctaaccc tcgaacagaa cgtccagttt gattcagttc aacaatcatt
     1141 tgtttaaaat tttcttcata tcgggtcata aaaattcatc ctctcgtaga taagtttaat
     1201 ttattcttgt ctctacgaaa agctgtctaa gatttgatac
//
LOCUS       DAYYGO010000249         1228 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000249 DAYYGO010000000
VERSION     DAYYGO010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.260"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(174..788)
                     /locus_tag="ACHA5T_002897"
     CDS             complement(174..788)
                     /locus_tag="ACHA5T_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8888402.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
BASE COUNT          400 a          240 c          213 g          375 t
ORIGIN      
        1 taacaaactt taaacaaaac aagcttaaaa tcaaagatga tccatcttaa aataactaaa
       61 acaaaaaaag tagtcgttgt taatcctcat taataatcgt tttctttttt tcaaacgctc
      121 catttttatg tggaacatta tttttttaag gaactttgtc aacatgtgga taactatttt
      181 ccacttgtgt cctggactag ctgttttgct gtaaaataca aaacttgctg cagcaaaaag
      241 atcgttgcaa gagaggctgt tccgcagatc gctggatcta gatttggcaa agtcaagact
      301 gcatcatgta aattggcgtg gttgtccaaa accaaatcag caaattcaaa taatcgcaaa
      361 ccactgctat gttgcgattt gatacttcgt gacaaatcaa atccagtaac aatgattaat
      421 ggatgaccat tttctttgat ccactgagct agttcgatga ttgccgggtt tcttccttca
      481 ttcgaaagaa gaagaaaaat gtcttctgat ttgacagaaa gctgatcaat aattgcctgt
      541 cctgcacctt ctagcgtttc atagacacct ccggctggat tttcaatcaa tatacttggt
      601 acaagatccg gggtttgatc ccaaaattca aatgctacac cacttagtgt gcggctagca
      661 tatatctgca atctaccacc ttgattaatt tgtttcgcga tccaagtgcc agcttgaagc
      721 atcagcggct cttctatcct taatttctct tccaattggt gataatagtc aaaataacat
      781 aaactcattt atctctcccg cctagctata cttgacatga atagaaacag ctgcctaatc
      841 taaggaagta cataaaaaaa cagtagttag acagtaacac atgtggtctt tgctgtttca
      901 aacacaaaaa aggcattgaa cgaatacgcc caataccttg acatagcaaa taaaacaacg
      961 tgcagaccat ggaaaatatc tacatagggg tagaattttc accctagatt aggtacaata
     1021 aattaggtcg atgcactgca ttgactatgg caaaatgatc ggtcagggtg tatgtgtgtg
     1081 actggtcatt ccctgattct tagcgccaac taagggtcag ggagcctttt tttattcagc
     1141 tgtttctatc tgaatttatt gtaagtaatc tcttatccaa gtgcaagcaa gaaaatacaa
     1201 aaaaacattg gtatgattta tatatata
//
LOCUS       DAYYGO010000250         1114 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000250 DAYYGO010000000
VERSION     DAYYGO010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.261"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..75
                     /locus_tag="ACHA5T_002898"
     CDS             <1..75
                     /locus_tag="ACHA5T_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFM8888403.1"
                     /translation="EFLFPKFLEDSSELQDIYNYLYRR"
     gene            80..1027
                     /locus_tag="ACHA5T_002899"
     CDS             80..1027
                     /locus_tag="ACHA5T_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFM8888404.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          356 a          170 c          237 g          351 t
ORIGIN      
        1 gaatttttgt ttcctaaatt tttagaagat tcttcggagc ttcaagatat ctataattat
       61 ttatatcgga ggtaaaagga tgagctgtta tcttatcacc ggtggggctg gcttcattgg
      121 ttctacctta gccaatcatt taggagaaga acatgtggta atcgtcgtcg atgacttatc
      181 aatgggaaaa gtagaaaatc ttgatatgac gaaaaatatc acttttattg agggagatgt
      241 cgctgagaaa tccttgatgg aagagatcat gaaagctaat aaatttgatt atatctttca
      301 tttagcagca gttgctagtg tggcagattc agttgcccga ccagtagaaa cacatcgtgt
      361 gaattttgaa agtgtattaa tgattttaga attgatccgc aagtatcaac ctgatttaaa
      421 acggatcgtg tttagttctt cagcggctgt ttatggggat gaaccgactt taccaaaaaa
      481 agaagagtcc gtgattcgcc cattgacgcc ttatgcggtt gataaattcg ctgcagaaca
      541 atatgtgcta gattattgtc atttatatga tgtacccggt agcgcagttc gttttttcaa
      601 tgtctatggt ccaaatcaaa atccgaattc tccatacagt ggtgtgattt ctattctcgt
      661 agatcgctat aagaaacaaa tcgtaggaga agcaacttca tttacattat ttggtgatgg
      721 aagccagtct agagactttg tttatataga tgatgtaatc caagcattac ttttagttgc
      781 gaatgaggaa gctgcactgg ggaaacaatt caatgttgga acgggtaaag caaccacact
      841 tttggaattg attcatgcaa tcaatgaaat tctcggtgta gaattgacac ttgaatacaa
      901 agaagagcgt tcgggtgata tccatgactc tctagcagat attactaaga tccagtcaat
      961 tggctatcaa ccgaaatatg gtattttaag tggaatgact aattatttga aaactgaaat
     1021 caattaaaac atactaagat aagattagag ggtaaattca ggctctttgt caataaggac
     1081 tgatgagttt tgtaaaatca aatctgggat agaa
//
LOCUS       DAYYGO010000251         1075 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000251 DAYYGO010000000
VERSION     DAYYGO010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.262"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..945
                     /locus_tag="ACHA5T_002900"
     CDS             <1..945
                     /locus_tag="ACHA5T_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFM8888405.1"
                     /translation="INDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNVAANMKNNY
                     NGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKALFEKNYRA
                     YIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYLSQMGVSKE
                     KLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGEIENANLHV
                     AGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYHVSFNMRNF
                     PSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          374 a          187 c          216 g          298 t
ORIGIN      
        1 attaatgacg agtataattt agcgccgaat caaggagact ccagattagc aattcctaac
       61 aaaattattt tgcacgaaac tggaatagat gcaccagcaa gaaacgtagc cgccaacatg
      121 aaaaataatt ataacggaag caattcttat actacagatg ttattggtga cggtgggatt
      181 gtttaccgtg tgggtgagca aggatatgtt tcgtggggag ctggtaacgc taatccttat
      241 gcgcctgtac agattgaatt acagcgcaca tatgataaag cattgtttga aaaaaactat
      301 cgagcttata ttgaatatac aagagatagt gcaaaaaaat atggaattcc attgactctt
      361 gatcaaggaa cttctttatt tacaaaagga atcatttctc atttgtgggt gacaaattat
      421 gtttggggga accacacaga tccatatggt tacttatcgc aaatgggagt tagcaaagaa
      481 aagcttgctt atgatttagc tcatggattt accgatgaaa atccaacaac ttctgaaaac
      541 aagcctgtca ttgatccaac acgagctggt gcagctaatc ctacgctgac agatggaaca
      601 aatcacgccc acattgatca gtttggggaa atcgaaaacg caaacttgca tgtggctgga
      661 tggcacattg ctaactataa atacgagtat attttcatta tggactacaa tactgggaaa
      721 gaattagctc gagtaagagc tgatggaatt tatagaccag atgtaaacca agcttataat
      781 acttctggaa atgttggtta tcatgtatct ttcaatatgc gtaattttcc tagtaagaaa
      841 gtctatgtaa tgatgcgggc aacgaatgat ccagagggaa acactaaagg cggtgcgcaa
      901 gatttccatg acaaacgttg gtatttaaat attcctaaac gataaaaata gctcctcgtt
      961 gaggagcagt acataactat attgacaact ataaaaatta ttcgataaaa tagtgatgtt
     1021 atcgcatatc ttcactatca cccataaata gtcacactcc aagctatgcg ataac
//
LOCUS       DAYYGO010000252         1047 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000252 DAYYGO010000000
VERSION     DAYYGO010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.263"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            91..831
                     /locus_tag="ACHA5T_002901"
     CDS             91..831
                     /locus_tag="ACHA5T_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888406.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT          387 a          182 c          201 g          277 t
ORIGIN      
        1 ttttcttaaa ataatcagcg cattgctact gaaaacaatg aatctgtgct attattgaga
       61 aaagttccat ttttgagaag gatggtttga atgaaagaaa tcagacgaaa acatttccgt
      121 tttcctgcgt acgatgatga gttaggcgta aaattaacca acgacaagaa gcgagtatta
      181 tttgacggac aagaagatct tttgactgaa cagaatcagt ccccgtttga acagatgaaa
      241 gaatttcatt tcacgaacac gaacaccgag aaaatccgtc gcaagcgtcg tgcaacaaat
      301 ggattgaagt cagctccgcg taaggaaaat ttggaaaagc ataaaggaaa attaccggat
      361 tataccggcc attaccaatc agaaatcaca tcgacaggac gaaaaaaact ttttggtgat
      421 tcctctcgtt ctgtcagaca aagtgaaaca aggacagagt ccaagcatac tacacgaaac
      481 gcatctgttg acacgaaggt gcgggagcgt tcttattttg ttcctaaata tgtacccgca
      541 tcgattatcc cagaccctag agaaagcaaa atttctgagg atcatttaat cgaatcgatg
      601 aaaaaaacgg atgactctta tctgttgttc gatacagaac cgacgccgta tcaagaaaaa
      661 agaaatggtg agccgtctgt acgaacattc aatcgaacaa aaacgttaga ggaaacaaag
      721 cagtcagaag aaaaaaaatc gaaaaaaagc catggtatct tagaacgctc attacaagga
      781 ttgatcgatg atcaaggaaa ctctttggga gaaaatagct attttaaata agtgatatac
      841 aaatttatat tcggctacag ttggaaagga aacgctgcgg cgtttcctta ttttatactc
      901 tatccgaaag tttatatcgt atcctcaaat ttttttgagt ttaagcgaat tttttctccc
      961 aataagtaag aaaataatgg caaaataaaa aaacaaacta ttttcgcaga tcgtttgcaa
     1021 aaatagttta tttttttata aagaaga
//
LOCUS       DAYYGO010000253         1043 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000253 DAYYGO010000000
VERSION     DAYYGO010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.264"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..980
                     /gene="bsh"
                     /locus_tag="ACHA5T_002902"
     CDS             6..980
                     /gene="bsh"
                     /locus_tag="ACHA5T_002902"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFM8888407.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          375 a          131 c          182 g          355 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaa
//
LOCUS       DAYYGO010000254         1037 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000254 DAYYGO010000000
VERSION     DAYYGO010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.265"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(20..91)
                     /locus_tag="ACHA5T_002903"
     tRNA            complement(20..91)
                     /locus_tag="ACHA5T_002903"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="ACHA5T_002904"
     tRNA            complement(113..200)
                     /locus_tag="ACHA5T_002904"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="ACHA5T_002905"
     tRNA            complement(225..298)
                     /locus_tag="ACHA5T_002905"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="ACHA5T_002906"
     tRNA            complement(312..382)
                     /locus_tag="ACHA5T_002906"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="ACHA5T_002907"
     tRNA            complement(384..459)
                     /locus_tag="ACHA5T_002907"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
     gene            complement(478..551)
                     /locus_tag="ACHA5T_002908"
     tRNA            complement(478..551)
                     /locus_tag="ACHA5T_002908"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(515..517),aa:Met,seq:cat)
     gene            complement(564..653)
                     /locus_tag="ACHA5T_002909"
     tRNA            complement(564..653)
                     /locus_tag="ACHA5T_002909"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(615..617),aa:Ser,seq:tga)
     gene            complement(677..750)
                     /locus_tag="ACHA5T_002910"
     tRNA            complement(677..750)
                     /locus_tag="ACHA5T_002910"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(714..716),aa:Ile,seq:cat)
     gene            complement(764..837)
                     /locus_tag="ACHA5T_002911"
     tRNA            complement(764..837)
                     /locus_tag="ACHA5T_002911"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(801..803),aa:Met,seq:cat)
     gene            complement(865..937)
                     /locus_tag="ACHA5T_002912"
     tRNA            complement(865..937)
                     /locus_tag="ACHA5T_002912"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(902..904),aa:Ala,seq:tgc)
     gene            complement(957..1030)
                     /locus_tag="ACHA5T_002913"
     tRNA            complement(957..1030)
                     /locus_tag="ACHA5T_002913"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(994..996),aa:Pro,seq:tgg)
BASE COUNT          281 a          285 c          246 g          225 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca gtgtttttag tttttaattg
      481 cgggagcagg atttgaacct acgaccttcg ggttatgagc ccgacgagct accagactgc
      541 tccatcccgc gataatatga aaaaggagga taagggattc gaacccttgc acggttttac
      601 ccgcctgacg gttttcaaga ccgttccctt cagccggact tgggtaatcc tccatatgaa
      661 acttacaacg ccacaatgga ccttgtagga ctcgaaccta cgaccggacg gttatgagcc
      721 gtctgctcta accaactgag ctaaaggtcc aggctcttaa aaatcgcggc ggaggggatc
      781 gaacccccga cctcccgggt atgaaccgga cgctctagcc agctgagcta caccgcgaaa
      841 aagatattca ttatttaaat gaaatggagc ctagcgggat cgaaccgctg acctcctgcg
      901 tgcaaagcag gcgctctccc agctgagcta aggccccgtg tttaaattta taattatcgg
      961 gaagacagga ttcgaacctg cgaccccttg gtcccaaacc aagtgctcta ccaagctgag
     1021 ctacttcccg tttaatt
//
LOCUS       DAYYGO010000255         1019 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000255 DAYYGO010000000
VERSION     DAYYGO010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.266"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..453)
                     /locus_tag="ACHA5T_002914"
     CDS             complement(<1..453)
                     /locus_tag="ACHA5T_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8888408.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIK"
     gene            complement(470..874)
                     /gene="tnpA"
                     /locus_tag="ACHA5T_002915"
     CDS             complement(470..874)
                     /gene="tnpA"
                     /locus_tag="ACHA5T_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFM8888409.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT          307 a          175 c          166 g          371 t
ORIGIN      
        1 tttgataatc ccttttggtt gtctatgaag cttaattctt actaatgact tcaatttagg
       61 aactttgatg aatttactat caatcaaagc aactgtacca ttttgattat tcgttgtata
      121 actctgaaca ggatttttct tacttttgaa acgtggaaat ccaacggatt tatcccgaaa
      181 aaaattcttg tatgctttat ctaaattaag ttgggcattg gctaaagcaa ggctatcaat
      241 ttctttcaaa aacggaaatt ctttcttgta tttagctggt gtcggaaaag tcattttttt
      301 agaagaatca tttttaactt cttcatacgc cttttttcgg tcatcaagca ttagattgta
      361 gaccttacgg acacaaccaa aagatttggc aaagaagatt tcttgttcct ctgttggata
      421 gattctgaat ttgtatgctt ttagttgttc cataaagttc acctactttc tatttatgat
      481 tgtttccttg attttggata tacttcttaa tcacatcaat tggtgcgcca ccagttgtca
      541 gaagacaaaa acttttagac cagaacattt ctttccaaag aaattgtttg actcttggaa
      601 agtcacgttt tatcaatctt gagctggcac ttttataggc attgatgaat tttgtcaatt
      661 ctgttttagg ttgagctttg aacataatat gaacgtggtc tttatcatga ttccactcca
      721 ctaaagtaat gtgatacgat tctgaaattc tttcaaaagt agttttggca taatcagata
      781 tttcatcatc aatcacttgt ctacgatatt tcgttactaa aacaaggtgg taataaagaa
      841 gaaatactga atgattattt gtatctaatt ccatgttata tcaactcatt tcttgtatag
      901 actgattgta acatggaaca aaataaaaag gcaatacgcc ttagttgtcg gtcttcgagt
      961 taccaacggt aaccctcata ccgaacggca ttcatcaccc acctatagag gatgggcga
//
LOCUS       DAYYGO010000256         1010 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000256 DAYYGO010000000
VERSION     DAYYGO010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.267"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..569
                     /locus_tag="ACHA5T_002916"
     CDS             <1..569
                     /locus_tag="ACHA5T_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFM8888410.1"
                     /translation="VSGDTIRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQE
                     NELAVSLPEVLKVRDSGIVYYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIA
                     NYFSVSETKLRESFKNEMHITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSH
                     FSRVFKKYAGVSPKQYQLGLVDNFNNIS"
     gene            666..>1010
                     /locus_tag="ACHA5T_002917"
     CDS             666..>1010
                     /locus_tag="ACHA5T_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888411.1"
                     /translation="MSCFVESERLSNGNNSVLNEYDKVVINKVKSLCEEARESISHIN
                     GSNFKQTLFNLIKLDAKISSYLFFLIHDEFMDYQKLDQLIERESWEDYYVGLTFSEKL
                     NNYKLIDYKYLSE"
BASE COUNT          370 a          108 c          184 g          348 t
ORIGIN      
        1 gtgttagtgg agatacgatt cggtctgaaa aaaattattc tatcattgtt tttgaaaagt
       61 tagcacaaac gtctataaca ttaggaatgg atataataga agcgtatcaa agtagagatg
      121 ctttgataca agaaaacgaa ttagctgtat cgttacctga ggtcttaaaa gtgagggatt
      181 ctgggattgt ttattatact aaggaaatcg gaaaaactaa gatagagcat ctttctccct
      241 taatttcttc tgtcgttcaa ttcatcggct taaatatcta taaaagaatt actgtaaagg
      301 aaatagctaa ttatttttct gtaagtgaaa ctaaattaag agaaagcttt aagaatgaaa
      361 tgcatattac gatttataat tatatctcta agcgaaaaat ttctacggct aaaataatgt
      421 tgaaatcaaa ccatactatc agtgaagttt ctttaggttt aggattttca gattcttcac
      481 acttttcgag agtattcaaa aagtacgcag gtgtatctcc taagcaatat caattaggtt
      541 tagtggataa tttcaacaac attagctgag aggttttatt ttattatgag actatatcat
      601 ttacgtagtt tggaggagtt gaggaaattg gattttaaca aaaaattttt tgggaaattt
      661 ctgatttgag ttgtttcgta gaatctgagc gtctaagtaa tggtaataat agtgtgttaa
      721 atgaatatga caaagtagta attaataaag taaaaagttt atgtgaagaa gctagagaat
      781 ctatcagtca tattaatggt agcaatttta agcaaacatt atttaatctg attaagctag
      841 atgctaaaat atcaagttat ttattctttt taatacatga tgaatttatg gattatcaaa
      901 aattggatca gttaattgaa agagaaagct gggaagatta ttacgtgggt ctaacttttt
      961 cagaaaagtt aaacaactac aagttaatag attataagta tttgagtgaa
//
LOCUS       DAYYGO010000257         1001 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000257 DAYYGO010000000
VERSION     DAYYGO010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.268"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..459
                     /locus_tag="ACHA5T_002918"
     CDS             <1..459
                     /locus_tag="ACHA5T_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888412.1"
                     /translation="AKYLKTHVEAIDGKEALVQTDGDQAIAGHKNFSGSVTINNKRVL
                     TTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGNDSVILTVPEGYR
                     TPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVITYSI"
     gene            473..904
                     /locus_tag="ACHA5T_002919"
     CDS             473..904
                     /locus_tag="ACHA5T_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888413.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            906..>1001
                     /locus_tag="ACHA5T_002920"
     CDS             906..>1001
                     /locus_tag="ACHA5T_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFM8888414.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITEE"
BASE COUNT          373 a          146 c          212 g          270 t
ORIGIN      
        1 gctaaatact taaaaacgca tgtagaagct attgatggta aagaagcatt ggtgcagaca
       61 gatggagacc aagccattgc aggacataag aatttttcag gttctgtaac aatcaataat
      121 aaacgtgttt tgacgactga tgataacaga tatgaagtgg taaatctggt tgtcactaac
      181 ggtaatacag ggacagcaaa gctttatcgt gaaggaaaaa cagtttctat atattttgtg
      241 gctttaaacg gaaaaagtag tggcgggaat gattcggtta ttttaactgt tccagaaggc
      301 tatcggacac caattagttt tgaacaactg gttgggtcaa tagaccgttc tactttgaac
      361 agtgctcaac tatctattgg cgcagacgga gccattaaat ggagaagaaa ttcaagttat
      421 ggatcagctt attcatttgt tatcacttat tcaatttaag ggaggaaatc taatgaaagt
      481 agtttacaaa tcaatcaagc cttacggatt cgagcaaatc attttgaaca atcaagaaaa
      541 tatccctgaa aactgtacag agattaaacc accagttcct aactggagac caagatttga
      601 ttttgataaa aaacagtggg ttgagttagc tactgaagaa gagaaaaaag ggaatgctgt
      661 tgatagcgta gatgaattgg ctaatattaa ggcattatac gaaacgctga aggcagaaaa
      721 tgatgaattg aaacagctaa attctaaagc aatgctaaac aatgtagcaa tcaagcaaga
      781 aaatgtctta ttgaaagaaa agtcagaaag tttagcgcag ttgaattcaa aaacgatgct
      841 tgcttctgta caaaatacca aggaaatcga agaaatcaaa aaacaattac aaggtgggaa
      901 gtaacatgta ttcatatgaa gatatcaaac taatgtatga ctggggctgt ttcactaacg
      961 aacaagtaat ggttttcgtt ccgttgtgca ttactgaaga a
//
LOCUS       DAYYGO010000258         1000 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000258 DAYYGO010000000
VERSION     DAYYGO010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.269"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            369..1000
                     /locus_tag="ACHA5T_002921"
                     /pseudo
     CDS             369..1000
                     /locus_tag="ACHA5T_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          181 c          183 g          294 t
ORIGIN      
        1 tttcaaagtt agtagagaaa atgtagttat atatttaaat ttttaccaga aagaaatgaa
       61 caccttcaat tatctaaatc tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt
      121 aaacgaatta ctattcatgg tcttagacac acacatgcta gcttgctttt agcaagctag
      181 tgcaaatatt aaagatgtgc aagagcgttt aggccactct tctattcaaa tcattatgga
      241 tctatatatt catattacag acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaata
      301 taggctctat aatttatggg actgatgaat caaaattgat tcttcagtcc cattgttatt
      361 ttaggtatat gattcgtaca ttgaaagagt tgaaaaacta tcttccgaag ccgtcaaaaa
      421 ggaccctgcc aaaagtattg atggtggatg aatttcgttc gcacgcttca attgtggata
      481 aaatgagctt tatttgtgcg gatggcgaga ctggaaaact cattgatatt ttgcctacac
      541 gaaaattgtc tcgattaaca cactatttta gtcgctgtgc aaatcctgaa gcggtggaat
      601 atttagtgac agatatgaac gccgcctact tccaactgac aaaacgtgtc ttaccaaatg
      661 cgaaagtcgt cattgattgt tttcatatca tcaaacattt gaatcaggcg ttcaatgatt
      721 ttcgtgtaca agaaatgaaa agattacacc agttagggca acgacgggaa gccgagaaag
      781 tgaaaaagaa ctggcgcttt cttctaaaaa accgtcaaaa aatcaatcac tatgattaca
      841 aaacatggcg cagttttcgt gcacctaaat acccgctact cactgaagca atgatgattg
      901 atcgattgct ggcattttca acgcctttaa aggacgccta tacttttttc catgagctga
      961 ccgaagcctt tagagacaag gaatcagact tattttttac
//
LOCUS       DAYYGO010000259          959 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000259 DAYYGO010000000
VERSION     DAYYGO010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.270"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(107..511)
                     /locus_tag="ACHA5T_002922"
                     /pseudo
     CDS             complement(107..511)
                     /locus_tag="ACHA5T_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
     gene            complement(570..>959)
                     /locus_tag="ACHA5T_002923"
     CDS             complement(570..>959)
                     /locus_tag="ACHA5T_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8888415.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          287 a          170 c          159 g          343 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttccattggg agcgtcaata attttgtgta
      541 aataaattgt cctcctgcaa aataattagt tactcagtaa acattgaaac taatgtatcg
      601 gttacctgtt gaaaaccttt atggcttctg tttagaaatt tttgattgta tgtatcaaaa
      661 atgctgacta gaaagcgttc tagtgattct tcattttgaa actgctcttt tctacggctg
      721 tatcttttaa tttgcttatt gaaagactcg attagattgg ttgagtaaat ggttctacga
      781 atgctaggtg gaaaatcata aaaagttaat aagtcttggt tttctatgag tgactgcgtc
      841 actttaggat agtttttctt ccatttctca atcatgccgg ataagaaggt attcgcttct
      901 tcttttgagt tagcttgata aacagcctta aagtcatcac agatttcttt tcggtcttt
//
LOCUS       DAYYGO010000260          943 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000260 DAYYGO010000000
VERSION     DAYYGO010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.271"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>943
                     /locus_tag="ACHA5T_002924"
     rRNA            <1..>943
                     /locus_tag="ACHA5T_002924"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DAYYGO010000261          940 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000261 DAYYGO010000000
VERSION     DAYYGO010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.272"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..>940)
                     /locus_tag="ACHA5T_002925"
     CDS             complement(37..>940)
                     /locus_tag="ACHA5T_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888416.1"
                     /translation="LKQYYPLSAYEKKKLFHFLAINLLAEQADSLDIASNQSLFFSDI
                     GFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNEDETIEWLNERFIESFENQF
                     NLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFFRENYPAFYCYLIEYINDL
                     TSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIIDFSYGAAYNQFIKKNLSLY
                     INLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDPPRAVDWGNLTKILLTIQE
                     EKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          305 a          160 c          145 g          330 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatcaa gtggaaattc
      541 atcagcttta ttaatagtgt aatgcaaaag tttaaaatgt attttggtta actctcttcg
      601 gatcaattgg ctcgtctctt ctcgcaacag attaaactga ttttcaaaac tttcaatgaa
      661 tcgttcattc aaccattcga ttgtttcatc ttcatttttt ctcaagtatt tttgatccaa
      721 tttatcatga agataaagcc aagagagtgt cttattgata aaaataacgg atttttctcc
      781 taatactcct tttaagtggt aaaatcctat atccgaaaaa aatagcgact gattgctagc
      841 aatatctaaa gaatcagctt gttcagccaa caggttgatt gctaaaaagt ggaataattt
      901 ctttttttca taagcagaca gaggataata ctgttttaaa
//
LOCUS       DAYYGO010000262          919 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000262 DAYYGO010000000
VERSION     DAYYGO010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.273"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..819
                     /gene="fabI"
                     /locus_tag="ACHA5T_002926"
     CDS             64..819
                     /gene="fabI"
                     /locus_tag="ACHA5T_002926"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFM8888417.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
BASE COUNT          288 a          174 c          183 g          274 t
ORIGIN      
        1 tagctattat tctctatttt tttacgacta tcaaattttt atcagaaaca aaggagagtc
       61 attatgtcat ttctagaagg aaaaaaaatc gtcattatgg gcgtcgctaa caaaaaaagc
      121 attgcttggg gttgtgctga agcaatgttg aaacaagggg cagaaattat ctatacttac
      181 caaaatgaac gaatgaaaaa atcattagtc aaattggtcg gcgaagaagc attcactgta
      241 gaatgtgatg tttctagcga cgaaagcatc gaaaaagcaa tgaatgccat cggtgaatat
      301 gctggggaga tccacggcct tgtccatgca gttgcttacg ccaacaaaga agaattgtcc
      361 ggaaatgtgt ctgatatttc cagagacggc tatcaattag ctcaagatat cagcagctac
      421 tctttaattg ctgtatcaaa atacgcacaa ccgtttcttg caccaaacag cgggatcgtt
      481 agcatgactt atctaggttc agaacgcgca atcccgaatt ataacatgat gggtattgcg
      541 aaagcttctc ttgaagctgc tatccgttat ttggcagctg agttctcgcc taaaggaatt
      601 cgggtcaatg gaatctcagc tggggcaatc aaaacattag ctgtcacagg tgttaaagac
      661 tatcaaaaac tgatccagtt atctgaatct cgtacgcctg atcagcaagg tgtgacgatc
      721 gaagaagtcg gtaatgcttg tgctttctta atcagcccac tttccagcgg aatcatcggt
      781 gatattatct acgttgacaa aggtgtccat ctttcataaa cttttgttgc attctttaaa
      841 gtaaaacata taaaaatcag gaaaaaactt tttaaagtct cttcctgatt ttttcttttt
      901 acgttttttt caactcatt
//
LOCUS       DAYYGO010000263          905 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000263 DAYYGO010000000
VERSION     DAYYGO010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.274"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..905
                     /locus_tag="ACHA5T_002927"
                     /pseudo
     CDS             1..905
                     /locus_tag="ACHA5T_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          330 a          157 c          172 g          246 t
ORIGIN      
        1 atcttacgcc tgaacaaaaa gaattgttag aactacgtaa aagaaaccag caattagaaa
       61 tggaaaatga tattttaaag caagcagcgc tgatattcgg acgaagagac aagtaatcga
      121 tgcgaataag catctttacc ctatatcagc gatgtgcaga atattaggtc tatcacgtca
      181 gtcctattat tatcaatcaa aaccaaagaa agacgaatca gaacttgaag aagtagtcgc
      241 tgaagaattt atccgcagcc gaaaggccta cggctcaaga aaaataaaaa aagccttatc
      301 aaaacgaggc attcagatca gccgacgaaa aattagtaga atcatgaaaa atagaggatt
      361 aaaatcgagc tatactgttg cttattttaa agtacatcat tctacttgca atgaagccaa
      421 aacgacaaac gtattgaatc gtaaattctt aagagacaac ccattagaag cgatcgtaac
      481 agacttgact tatgtacgag tcgggaaaaa atggaattat gtctgtttca ttttggatct
      541 gttcaatcga gaaattctcg gctattcttg tggagaacat aaagatgccg ttctagtaaa
      601 aaaagcattt agccgtatca aacaacctct gacagaggtt gagatttttc atactgatcg
      661 tggaaaagag tttgataacc aagctattga tgaattatta acaacttttg acatcaatcg
      721 atcattgagt cataaaggct gtccttttga taatgccgta gctgaatcaa cttataagtc
      781 gttgaaagta gaatttgtct atcaatacac atttgaaacc ttacaacaat tggatttgga
      841 gttatttgac tatgtcaatt ggtggaacca ccttcggttg cacggtacac ttggctacga
      901 gacac
//
LOCUS       DAYYGO010000264          902 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000264 DAYYGO010000000
VERSION     DAYYGO010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.276"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            95..415
                     /locus_tag="ACHA5T_002928"
     CDS             95..415
                     /locus_tag="ACHA5T_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8888418.1"
                     /translation="MNTYEIVKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            433..864
                     /locus_tag="ACHA5T_002929"
     CDS             433..864
                     /locus_tag="ACHA5T_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFM8888419.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
BASE COUNT          323 a          124 c          156 g          299 t
ORIGIN      
        1 gatatagtat agctcattac tcagaattag tcaaattatt tctgcgtatt tttctaaact
       61 ttttttgtaa acatttagaa aggttgtttt atcgatgaat acctatgaaa tagtaaaaga
      121 cttggcgaag aggaaaaaga tatctatacg gcagttagaa attaattttg gatattctaa
      181 cggttatcta gcaaagtgga aaaataatac tcctaattca gatgaattaa gtcggattgc
      241 tgactatttc gatgtctctg tagactacct tctaggtaga gaagaaagag aaacccctaa
      301 acatgtggat ttatcagaag acgatactgt tttttctttt gatggaaaag aaatatctaa
      361 ggagacaatg cgtaaagcga ttgcaattgc taaagcttta gaggaaaatg aatagttgga
      421 gtgatggttt gtatgtattt gaaattgaaa gaaatgttga gcgaatacaa tttgaaagta
      481 gtttatatgg agatgaaaga gcctggtttc tactatccaa aacccagaat catttttttg
      541 aatgaaaatc tatacggtga aactgcagaa gcctttcatt tatctcatga acttgcacat
      601 ttcagcgcct ctcattttga attttcagtt ttgtacgata cttctacaac ttttcactca
      661 aaatttgaaa cagaagcaga taaaatagct attctaattt tattaaatat ctatattgag
      721 aacgaactga ctgatgaatc tcagtttaat ctcgaaaaat ttatggagta ttattctatt
      781 cagaataagc tcagatacac ttgttatgct gtctgccaat gctattttaa gaaaaaatat
      841 tcttacgcaa ggcaatatgt atgaatacat caagaattat taaaaaaacc gatttcagca
      901 aa
//
LOCUS       DAYYGO010000265          877 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000265 DAYYGO010000000
VERSION     DAYYGO010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.278"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            136..309
                     /locus_tag="ACHA5T_002930"
     CDS             136..309
                     /locus_tag="ACHA5T_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888420.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
BASE COUNT          290 a          144 c          106 g          337 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaaatgc actccacacc ttcatactct
      841 atcatttttt ataaacgata tatttttatt gtttttc
//
LOCUS       DAYYGO010000266          869 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000266 DAYYGO010000000
VERSION     DAYYGO010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..869
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.279"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(56..128)
                     /locus_tag="ACHA5T_002931"
     tRNA            complement(56..128)
                     /locus_tag="ACHA5T_002931"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(93..95),aa:His,seq:gtg)
     gene            complement(131..204)
                     /locus_tag="ACHA5T_002932"
     tRNA            complement(131..204)
                     /locus_tag="ACHA5T_002932"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(170..172),aa:Trp,seq:cca)
     gene            complement(211..291)
                     /locus_tag="ACHA5T_002933"
     tRNA            complement(211..291)
                     /locus_tag="ACHA5T_002933"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(255..257),aa:Tyr,seq:gta)
     gene            complement(293..368)
                     /locus_tag="ACHA5T_002934"
     tRNA            complement(293..368)
                     /locus_tag="ACHA5T_002934"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(333..335),aa:Phe,seq:gaa)
     gene            complement(404..476)
                     /locus_tag="ACHA5T_002935"
     tRNA            complement(404..476)
                     /locus_tag="ACHA5T_002935"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(441..443),aa:Asp,seq:gtc)
     gene            complement(486..558)
                     /locus_tag="ACHA5T_002936"
     tRNA            complement(486..558)
                     /locus_tag="ACHA5T_002936"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(523..525),aa:Val,seq:tac)
     gene            complement(574..645)
                     /locus_tag="ACHA5T_002937"
     tRNA            complement(574..645)
                     /locus_tag="ACHA5T_002937"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(610..612),aa:Glu,seq:ttc)
     gene            complement(660..749)
                     /locus_tag="ACHA5T_002938"
     tRNA            complement(660..749)
                     /locus_tag="ACHA5T_002938"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(713..715),aa:Ser,seq:gga)
     gene            complement(789..862)
                     /locus_tag="ACHA5T_002939"
     tRNA            complement(789..862)
                     /locus_tag="ACHA5T_002939"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(826..828),aa:Asn,seq:gtt)
BASE COUNT          242 a          238 c          199 g          190 t
ORIGIN      
        1 tgagggagtc aaagtccctt gccttaccgc ttggctatac cccaagaata aaaagggcga
       61 atgatgggaa tcgaacccac gaatgccgga gccacaatcc ggtgcgttaa ccacttcgcc
      121 acattcgcca tggcaggggc agcaggaatt gaacccacac taacggtttt ggagaccgct
      181 gttctacctt taaactatgc ccctataaaa tggaggagag tggattcgaa ccactgaacc
      241 ctaaggaacg gatttacagt ccgtcgcgtt tagccacttc gctactcctc catggtggcg
      301 cgggacagaa tcgaactgcc gacacacgga gcttcaatcc gttgctctac caactgagct
      361 accgcgccat attttaaaat tattaattga ataacaagaa cgacggttcc gacgggaatc
      421 gaacccgcga tctcctgcgt gacaggcagg catgttaacc cctacaccac ggaaccagtt
      481 cattatggag gttaacggga tcgaaccgct gaccctctgc ttgtaaggca gatgctctcc
      541 cagctgagct aaacctccaa cattcaatta aaatgacccg tacgggattc gaacccgtgt
      601 taccgccgtg aaagggcggt gtcttaaccg cttgaccaac gggccaggta aatttgctac
      661 ggagagtaag ggattcgaac ccttgagaca gtttggcacc atctacatga tttccaatca
      721 tgctccttcg gcctctcgga caactctcca gagtgaaaga agctcaatac tctttctcaa
      781 aatgagaact ccggcagtag gactcgaacc tacgacatca tgattaacag tcatgcgcta
      841 ctaccaactg agctatgccg gaataacat
//
LOCUS       DAYYGO010000267          850 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000267 DAYYGO010000000
VERSION     DAYYGO010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..850
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.280"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>850
                     /locus_tag="ACHA5T_002940"
     CDS             <1..>850
                     /locus_tag="ACHA5T_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM8888421.1"
                     /translation="VISYIQSYFTINFNHFKYLILSFISTFLNIAISVALMQTLYFDD
                     KYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYALKISLPLIPHSLANIVLA
                     QFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWVPWFYGEMSKNNEKKVKK
                     VSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVPITLGYFFQFLYSLPVNA
                     EFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTTVFTYFLLFIFHYNIAKK
                     ITNKQL"
BASE COUNT          268 a          107 c          142 g          333 t
ORIGIN      
        1 ttgtaattag ttatatccag tcttatttta cgattaactt taaccatttt aaatatttga
       61 ttttatcttt tatatctact tttttgaata ttgcaatctc tgtagctttg atgcagacat
      121 tatattttga tgataaatat gttggcagag tagtcggcgg aacactgtca tttgtgttga
      181 taggtttagt aatttattta agaataatga taaagggtgc tcattttttt gataaaaaac
      241 agtggagata cgctttaaaa atctctttgc cgctaatccc acattcctta gcaaatatag
      301 ttttagcaca attcgataga attatgatta attcttattt aggtcctgct gcggggggaa
      361 tatattccta cattagtaac ataggtatta ttctaagcgt aatatggatg tctactaata
      421 acgcctgggt tccatggttc tatggtgaga tgagtaaaaa taatgaaaaa aaagtgaaga
      481 aagttagtaa ctattattta gtagtcttta ctttgattac gatgattgta atggtagttt
      541 cgattgattt agcaaaattt atggctccga aagactatta ttctgggtta tctttagtgg
      601 ttccgataac cttaggctat ttctttcagt ttttatatag tttaccagtt aatgctgaat
      661 tttatgagaa aaaaacatcg tttattgcga ttggtacagt agcaagtgct atagttaata
      721 tactattaaa catgattttc atacctaggt taggaataat tgctgctgga tacacaaccg
      781 tatttacgta ttttttactt tttatttttc attataatat cgctaaaaaa attacgaata
      841 aacaattatt
//
LOCUS       DAYYGO010000268          846 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000268 DAYYGO010000000
VERSION     DAYYGO010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..846
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.281"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..300)
                     /locus_tag="ACHA5T_002941"
     CDS             complement(<1..300)
                     /locus_tag="ACHA5T_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016438386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HFM8888422.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRY"
     gene            complement(527..>846)
                     /gene="mef(H)"
                     /locus_tag="ACHA5T_002942"
                     /gene_synonym="mefH"
     CDS             complement(527..>846)
                     /gene="mef(H)"
                     /locus_tag="ACHA5T_002942"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
                     /protein_id="HFM8888423.1"
                     /translation="LFLELPAIVSYWVVTVMCFVAMGVSTMFVVQIYTLVQTQTPPQL
                     VGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVASIASIMISIYSKRIFQK
                     LEI"
BASE COUNT          294 a          167 c          115 g          270 t
ORIGIN      
        1 ataacgtaat aaaatcgcac tacgctgttt ttctgacagt ctatgtaaaa ctgtaccaag
       61 tgtttcatca gatacataaa ttgtaaagtg caagacttta aatgatacac aatcataaaa
      121 acaggtatca atcgttccac gttcaacttg tttcgtttcg gctatttcac aaaacaatac
      181 ttcacgttta gaatgtcttg caagctgtct gtttcgactt ttcactttgt tcttaatgac
      241 aatcagcatt aaagaattga actgtaatct gacgatatgc tcgaaagaag atgtcctcat
      301 aatctcaccc cctttctgct aatgctgaaa tatataaacg gaataccgtt tatacccttt
      361 agaaatacag aaaaatggta agaaaaaagc ccacacaaaa catatatttg tgcgagcctt
      421 aaatactgca atattactct ttcttttata acactatcct tttagcagat ttcaaatttt
      481 cttttatata ttgtttttag cctaacatac tatttaaccc cttgtattat atctctaact
      541 tttgaaaaat tctttttgaa tatattgaaa tcatgattga agcaatagaa gctacaaaca
      601 taacaaccca tgtttcttgt ataaatacat cgaataaaat accgtaaacc atttgtccaa
      661 taggttgacc gcacatagca actgctacca gcgtagccat tatctttcct actaattgcg
      721 gtggtgtttg tgtctgaaca agagtgtaaa tttgaacaac aaacatagta gatacaccca
      781 tagccacaaa gcacatcact gtaactaccc agtaggaaac tattgcaggc aattctaaaa
      841 atagcg
//
LOCUS       DAYYGO010000269          824 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000269 DAYYGO010000000
VERSION     DAYYGO010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..824
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.282"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..482
                     /locus_tag="ACHA5T_002943"
     CDS             <1..482
                     /locus_tag="ACHA5T_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888424.1"
                     /translation="EVFVGDKCIFFDQPLLSDGYGIDETDIFAKVNSIFSGKKETLIK
                     LHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTLLISPISTISFSPSMMFEKTINSI
                     MLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLSEEKILLPENWAELRKLVEYGII"
     gene            469..>824
                     /locus_tag="ACHA5T_002944"
     CDS             469..>824
                     /locus_tag="ACHA5T_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM8888425.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMN"
BASE COUNT          277 a          113 c          122 g          312 t
ORIGIN      
        1 aagaagtttt tgtcggagat aaatgtatct tttttgatca acctttatta tctgatggtt
       61 atggcataga tgaaacagat attttcgcta aagtaaatag tatattttct ggaaaaaaag
      121 aaactttaat taagcttcat cctagagcaa ggacaaatcg ttatggtgaa aaaaacatat
      181 tgaattccag tttaccgttt gaagtcttgc tacttgaaaa tgacttctct tcaacacttt
      241 tgattagtcc tatatctaca atttcttttt caccatctat gatgtttgaa aagacgatca
      301 actctattat gttggctaaa ttaattttaa atgaatatac agcattatct gaagataaaa
      361 aagaaacatt gacaaatatt gttcttttct gtgaacatta taaccgtcta tcagaagaaa
      421 aaattttact cccagaaaac tgggcagaat taagaaagtt ggtagagtat gggattattt
      481 aacacacttt ccaagtcaaa attagttaag tccagtttct ggtatactat agggaatttt
      541 tttattaagg gaatttcatt cataactatt cctatttttg taaatattac tactcaagca
      601 gaatatggtt tgattaataa tttcacttca tttgtatcaa ttttttcgat tcttgttggg
      661 ctaagcctaa attcggcaat taataatgca agttttgact ataaagatga cataaatgaa
      721 tttatgtcat cggtactttc tctagctaca ctttcgttaa ttgtgttctt attaataggg
      781 aacatatatt ttttgttttc aaatgctttt tttgatatga acca
//
LOCUS       DAYYGO010000270          797 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000270 DAYYGO010000000
VERSION     DAYYGO010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.284"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..651
                     /locus_tag="ACHA5T_002945"
     CDS             <1..651
                     /locus_tag="ACHA5T_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HFM8888426.1"
                     /translation="SEKVIDICENKILTSAFFEQNGFKTPEILNSSEITKFPVFIKPL
                     NGSSSVNTFIVNNEKELLFFSEYIDNPLIQEYILGEEYTIDVCLNFDSTPVSIVPRKR
                     IATRGGEILKGQIIKDRELIETTKKMLEKLNAIGHITIQCIKNEKGIFYLEINARYGG
                     GAPISIKSGANSPEYIIKMIDSKKNLRYRENYLDKMIALRYDQAIYLDEKGNVYND"
BASE COUNT          311 a           90 c          139 g          257 t
ORIGIN      
        1 tcagaaaaag tcatcgacat ttgtgagaac aaaattttaa cttctgcttt ttttgaacaa
       61 aatggtttta aaactcctga aattttaaac tcttctgaaa ttacaaaatt tcctgtattt
      121 ataaaacctc tgaatggtag ttcatctgtt aacacattta ttgtaaataa tgaaaaagaa
      181 ttgttgtttt ttagtgaata tatagataat cctcttattc aagagtatat tttaggtgaa
      241 gaatatacta tagatgtttg cttaaacttt gattcgacac cagtaagtat tgttcctaga
      301 aaaagaatag caactagagg tggggaaata ctgaagggtc agattattaa agatagagaa
      361 ctaattgaga ctacgaaaaa gatgttggaa aaattaaatg caatcggaca tattactatt
      421 caatgtataa aaaacgaaaa gggaatattt tatttagaaa taaatgcaag atacggtgga
      481 ggagccccca ttagcattaa atcaggagct aattcccctg aatatattat aaagatgata
      541 gattcaaaaa aaaatttgag atacagagaa aattatttag ataaaatgat agcactgaga
      601 tatgatcaag cgatttattt agatgaaaaa ggaaatgtat ataatgatta aagcggtgat
      661 ctttgattta gatgacactc ttgtgtctga atatgattat gtaaaaagtg gatacaaatg
      721 tgtagctaga agattagcag tgcaaaaaat tttcccggct acttttgacg aaatatatag
      781 agtcctttta gatcttt
//
LOCUS       DAYYGO010000271          787 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000271 DAYYGO010000000
VERSION     DAYYGO010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.285"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..717
                     /locus_tag="ACHA5T_002946"
     CDS             1..717
                     /locus_tag="ACHA5T_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFM8888427.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
BASE COUNT          282 a          151 c          159 g          195 t
ORIGIN      
        1 ttgaaaaaaa ggattttagt aacaattggt ctagcttttt tgttagctgg ttgtaccaac
       61 gggaatgaat cgaaaaaaga atcaacaaaa gaaacagcga aagcaacagt tgcaactact
      121 acaacaacta gcacagagag caagtcaact gcagcaagta cagaaacagc agatacctct
      181 acttcgtcag agtcttccaa aacgactgaa acaactcaaa catctacttc aagttcttct
      241 gtagaggctt catcttcctc ttcagaaagc agcgaacaag ttgaacaaac actgtggaat
      301 gcagacaaat ccgcacaatt agaggcattt atgaccaaat ggggccaaac aatgggacaa
      361 caatacaaaa gttatacgaa tcaaatgagt gtggacttat atggtcttaa agtccctcaa
      421 gctattttga acggggagtg gaagatggct attggtggag tgcctgtttc tgcagaatgg
      481 tcagaaagtg ggacaggtca agcagactat caaatcacag ccgtctattc cgatgcagag
      541 acagaaccat atctgaaaaa gcacgtctat ctattcgggt ttcagcaaaa tcagccaaaa
      601 gtattggtga cacaacaaaa tcaggggaat cccgataatt atctttactt caacgagaca
      661 gcgaataatg aattgaaaaa tggctttaat caaatcgttt atggacaagc aaattaatga
      721 gataaacaag gggttgtgac atgtctttta tcacaacccc ttgctcagtt tatttgttag
      781 tctttta
//
LOCUS       DAYYGO010000272          783 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000272 DAYYGO010000000
VERSION     DAYYGO010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.286"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            44..>783
                     /locus_tag="ACHA5T_002947"
     CDS             44..>783
                     /locus_tag="ACHA5T_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFM8888428.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEG"
BASE COUNT          308 a          127 c          147 g          201 t
ORIGIN      
        1 ttgttaaatt tgtttacgaa tataaaagaa aggaatgaga aatatgaaca caccgcaaat
       61 ttttaatttc gaacaaaacg aagttcggac aattttagta aatgatgaac catattttgt
      121 aggcaaagac gttgcgagtg ttttgggtta ttcaaacact aaagatgctt tgtcgcggca
      181 tgtagatttg gaagataaga tggggtcgcg aatcacgacc tcaggtcaat caagagagat
      241 gacaatcatc aacgaatctg gtttgtacag tttaatctta aaatcaaaac ttccctctgc
      301 caaaaaattt aaacgttggg taacaagtga agtgttgcca gcaattagaa aacatggagg
      361 ttatctaact ccagaaaaag tagaagaagc tttgcttaat ccagatacaa tcattcaatt
      421 agcaactcaa ctaaaagaag aaagaactgg aagattaatc gcagaacaaa agattgcaga
      481 gtacgaacct aaaatctcct atttagatag catattatct tctacagatt cagtaacaat
      541 tagtcagatt gcagcagatt atgggatgtc tccacaacag atgaataaat tacttcataa
      601 actaggtatt cagaaaaaag tcggtaacca atggttattg tgcaaaaaac acatgaacca
      661 aggatacaca aaatctcata caactgagat cccgaaagcc gatggtggca ctaaaattgt
      721 aatgaatacc aaatggacac agaaagggcg tctatttatc tacgaattac taaaaaaaga
      781 agg
//
LOCUS       DAYYGO010000273          774 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000273 DAYYGO010000000
VERSION     DAYYGO010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.287"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            411..641
                     /locus_tag="ACHA5T_002948"
     CDS             411..641
                     /locus_tag="ACHA5T_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888429.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
     gene            647..>774
                     /locus_tag="ACHA5T_002949"
     CDS             647..>774
                     /locus_tag="ACHA5T_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM8888430.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL"
BASE COUNT          268 a          123 c          128 g          255 t
ORIGIN      
        1 gaacactttt gtctcacgct ctaaacacgt ataaacagcg gaagcagaag caactccttc
       61 ggaaataagc tgaaattcac aaaaatttga aaagcaattt ccgtgaattt cttcttattt
      121 cttggagtta aacgcttctg ttccagcctc tttttatctg tctttcacta atgatcagtt
      181 gtagataagc aagttgcttt ttcttacatc ataccgccca tcaagacaaa cgtttttagc
      241 ttgtacgtac tggacaaaaa tcccattata tcaagaaaaa acaacgttga taatacttaa
      301 aattattagg ctagaacgga ctagtaagag ttttttgccc cttttttgcc cctcaaatat
      361 cgctttacag ccagaacata agttcgtata atcgttttga ggtgattttt atgatggaag
      421 actttattag aaaaaatatc agtgatgaat atgcagactt ttatgagcaa agcagcaaaa
      481 aagataaatt ccagatggat gtttcaattt tagctatatt agctttttcc gaaaataaac
      541 aacctgtaac tgcaaaaaaa gaaacagtat tctctgaagg caaaataaaa actcgatata
      601 tattagaggt agagactaag tttaaaaata gatcggagta atggttatgc tttttaatga
      661 gacgcaatta tggtttaaat ttgatccttc gaatagattt gtcaaagatt tttataaggt
      721 gtgggattca gaagttttct ttttagcaat cgaagatagc ttattaatta atct
//
LOCUS       DAYYGO010000274          771 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000274 DAYYGO010000000
VERSION     DAYYGO010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.288"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            116..482
                     /gene="ssrA"
                     /locus_tag="ACHA5T_002950"
     tmRNA           116..482
                     /gene="ssrA"
                     /locus_tag="ACHA5T_002950"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT          251 a          126 c          150 g          244 t
ORIGIN      
        1 aaaggggcag ttattatata aataatggga tatgattcgc ttttttattg aatttatccc
       61 aatatatgct atactaattt tgtatgaaaa atacacgtaa cccttgttag ctaacgggga
      121 cgttacggat tcgacaggca cagtcgagct tgaattgcgt ttcgtaggtt acgtctacgt
      181 aaaaacgtta cagttaaata taactgctaa aaacgaaaac aactcttacg ctttagctgc
      241 ctaaaaacag ttagcgtaga tcctctcggc atcgcccatg tgctcgagta agggtcctaa
      301 ctttagtggg atacgtttca actttccgtc tgtaagttga aaaagagaac atcagactag
      361 cgatacagaa tgcctgtcac tcggcaagct gtaaagtgaa tccttaaatg agttgactat
      421 gaacgtagat ttttaagtgg cgatgtgttt ggacgcgggt tcgactcccg ccgtctccat
      481 atatgtatgg ttgagacaga taaacatgta tgaaaaacgt tgttaaatca acgttttttt
      541 attttggtca aggagagaaa agtagagaaa tacaaaattt tttgcattga ttttgcattc
      601 aaaaaaacat tcaaacattt agaaatcccc aatacgtatt caggaaaatc tattttaagg
      661 tttacaaaaa gaacttttgt tcgtacactc tttttgaggt gaacttttat gctgatggaa
      721 ggaaaaacac aattatggtt taagcatgtt ccttcaaata agttttttta a
//
LOCUS       DAYYGO010000275          762 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000275 DAYYGO010000000
VERSION     DAYYGO010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..762
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.289"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            7..>762
                     /locus_tag="ACHA5T_002951"
     CDS             7..>762
                     /locus_tag="ACHA5T_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8888431.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEENTLCSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFLNRLTPYALILIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQK"
BASE COUNT          259 a          170 c          133 g          200 t
ORIGIN      
        1 aaacaaatgc cttacattct tgcattagat gtttcaatgg gacacagcta cagtgtccta
       61 tacaaagacg acacctgctt atttgaagac gagatcgaac acactcaaag aggatttcat
      121 gcattactcg aagagatcca gcacttgcct gaacgtccat tgattgtttt tgaagcaacg
      181 gggatttatt ccagaccagt cgaaaagttc tttcaagatc atcacttttc ttactgtttg
      241 ttaaacccgt tagaagcgaa aaaacagatg gaagaaaaca ccttatgcag ttggaaaaca
      301 gataaatcgg atgcccatcg gctcgcacag actcatctta aaaatcaacg tcagccaaaa
      361 gaagtacaaa gcaattttta cttagagatg cgcgatttag cccgttttta tcaagaaatt
      421 gagaagaaaa tcactcgtct gcgcatggat ttacacaact gccttcagtt gacttttcca
      481 gagcttgaac agttcttttt aaacaggctg accccttacg cattgatatt gatccgtctt
      541 tttccccatc cagattttgt attggcatct actcggacaa aaataaaaaa taaactgatc
      601 aacgaaacac ggaaaaaaat ctccgcgaat cgtgccgagc aaaaggcaga tcaaatcatc
      661 cactatgcac agtgtgccta tccagctgtt gaaaaagaca gtatccactg tcaaaaaacg
      721 atctattacg ctgaacttct ccaagattta ctcgaacaaa aa
//
LOCUS       DAYYGO010000276          717 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000276 DAYYGO010000000
VERSION     DAYYGO010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.290"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>717
                     /locus_tag="ACHA5T_002952"
     CDS             <1..>717
                     /locus_tag="ACHA5T_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888432.1"
                     /translation="HYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMS
                     ILPLILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKK
                     IEIFLIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIG
                     SIDSELLSFGIGLTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQN
                     TPLNIIQAPWRFMIFSTLFIS"
BASE COUNT          233 a          100 c          102 g          282 t
ORIGIN      
        1 tcattatgta atgtatgaaa ttaccaaaaa acatgttact agctcctttt tcgctattat
       61 atatacaaca tcttctttta ggagtgtaga aatatttctt cgaggggcaa tgggggaatt
      121 actcgcaatg tcaatacttc ctcttatttt actcgggttt ataaagcttt acgactcaaa
      181 aaaagaatct tgggttatgt tggcgattag catgacttta ttaatttaca ctcatgttct
      241 ttctgtagct atgactatgg taatgattat aattgcttta attattcaat tttatcggaa
      301 aaagaaaata gaaatcttct taattattaa attaataaaa gctgctatag ttactttact
      361 tctttcacta gcgtttatag gtcctatgat tgagcagaca ttgtatcaag atctgaatag
      421 accttatgtg gatatattac aaaaaagagc aatttatctg ggtaaagagt ttttgattgg
      481 aagtatagat tcagaattac tatcatttgg tattggctta actttgctta tttgtttaat
      541 tatactaaca tttaacttta aaaaattagg aatcttatct aaaatcactt atataatggg
      601 tataatatct attctacttt gtacaaaaat atttccatgg tttgccttac aaaatactcc
      661 tttaaatatt attcaagctc cttggcgttt tatgatattt tcaacattgt tcatatc
//
LOCUS       DAYYGO010000277          711 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000277 DAYYGO010000000
VERSION     DAYYGO010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.291"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(245..358)
                     /locus_tag="ACHA5T_002953"
                     /pseudo
     CDS             complement(245..358)
                     /locus_tag="ACHA5T_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          261 a          106 c          121 g          223 t
ORIGIN      
        1 aaagagtata cgaacaaacg ttctttttgt aaaggtggaa tttattatac taaataaata
       61 ggtgaacaag tacttgtgcc aaattatgtg ccaaaaaaaa tcgaatttaa taaaacacaa
      121 acaaaaagaa atcctattat gataacattt cttataataa caaacacaac agaagacgtg
      181 taatagttag tcaggaacgt acaaataacc cctgtatcct ttgcggtaca gggggttatt
      241 ttgtataagt agacattgta gtttctaaac tgctatgtcc taaacgtttc gatacgctaa
      301 gtatatttac tccttgataa agtaaaatag atgcatgcgt atgtcttagt ccatcaataa
      361 acagtcgaag agaaaatagg gaaaaattga ttactaaaga aaaacttact aggtcgacgg
      421 tgtagttgat tttcatactt caatagattc gaattaaacg atttcgattg gcttatacca
      481 gtcctatcca acgaaagata tatcgttctt aactctagcg gtataacttg aagctttacg
      541 taaagccaat gggaatgaac tggagatgac tttttattat ttttagtaat agctgatggg
      601 ttaatactta caactttcct tttcaaagta ccatataaaa aaacggtggg gttgtttagc
      661 gaaataaagg atctaagcca atagcttaga tcctttattc tatgctttat a
//
LOCUS       DAYYGO010000278          705 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000278 DAYYGO010000000
VERSION     DAYYGO010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..705
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.292"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            298..>705
                     /locus_tag="ACHA5T_002954"
     CDS             298..>705
                     /locus_tag="ACHA5T_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8888433.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNL"
BASE COUNT          234 a          138 c          111 g          222 t
ORIGIN      
        1 taaaaacacc agatttcaaa tttcacacaa tttaaactag atcaatcatc aaccttcttc
       61 aaattgttag aaccctttcc cgaggagctg cagcataagc aaaaaaacaa acaggaagcc
      121 aaatggctaa gacatttttt ctattagtct agttgcttct cgtcgcaagt ttaagacttt
      181 agaaagttat ttctaatgat gtttgggttg ttttgatcaa tgcttatgca cagctcctta
      241 cctattattt atctcaacag atctaacaaa ttaatggact atttaaggag tttttttatg
      301 cttacgatct attcttctcg cacacatagt taccatctga tggcaaactg gctgaaagac
      361 catcaaatgc cttacagaga acgaattttc acacctaaac agcctttaac aaggaaagag
      421 atattttatt tattgtcatt aacagaaaac ggatttgacg atctattagc taaacgctcg
      481 attgattata aaaatttgaa agaccaaatc gaccaatgta cgacctcaga gcttgtcgat
      541 ctgatcgtct cgcattttac tttgttacaa cgtcccatca ttactgacgg aaagaaatta
      601 ttagtcggat tcaatgaaga tagcatgcgt ctttttatcc ctgtacaatg gcgcagaagt
      661 caactgaaat ctgtctataa cgattttaca tgggtaagca atcta
//
LOCUS       DAYYGO010000279          704 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000279 DAYYGO010000000
VERSION     DAYYGO010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.293"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(60..530)
                     /locus_tag="ACHA5T_002955"
     CDS             complement(60..530)
                     /locus_tag="ACHA5T_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFM8888434.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
BASE COUNT          266 a          108 c          111 g          219 t
ORIGIN      
        1 atctaactta ggtggcactt tttctatgtg ttaaattaca ggacgtctct ttttagcgct
       61 tagtttaaaa aataaattga ataattcaat agagttgcga atagtaccca taaataataa
      121 ggaatcatga gccacattgc caggcgatga tgcagccaca actgatacat aagcactgac
      181 aagaaaataa ctaaaagtgt aatatcgatt gctgcaatga aaatatttct taaattaaaa
      241 aatagagcag accatataaa attaaaaatg aactgaatca caaaaagacc gacaagattg
      301 tttcgctgtt ttttgttgtt gtgattaagt aacaaataaa gagaaatacc catcaatata
      361 tacagtaagg tccacatcgg cccaaatatc cagctaggtg gtgcaaatgg tggaagttgc
      421 aaagaatagt aatattttcc gggatttccg ctaagcaacc ctgataaaaa gcctaatatg
      481 acaattccta taacacaaac ccaaaaacga atatctttta tcatcttcat tttactaact
      541 cctttctata atattagttt accatgaagg ctcacagcaa gacctgtatt tagtttgtga
      601 agtaagaaaa aaattgataa ttgttgttat taaaaagttg aacatttgtt tatatgaatg
      661 atgtttttga aaaagatcat atgatttgat tctcatgttt tcta
//
LOCUS       DAYYGO010000280          700 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000280 DAYYGO010000000
VERSION     DAYYGO010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.294"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            37..>700
                     /locus_tag="ACHA5T_002956"
     CDS             37..>700
                     /locus_tag="ACHA5T_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8888435.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFK"
BASE COUNT          282 a          108 c          113 g          197 t
ORIGIN      
        1 aatatcagtt aaaataaaag ttaaggagca ttgtgtatga caattaaaac tagtctccaa
       61 aatcaagctt accaatctat tcgccgacaa attatctatg ctgacttaga acctggaaaa
      121 aaagtatcag aaaaaaaatt agaagaaaca ctttctattg gtcgtacacc aatacgtgaa
      181 gcactgattc aactccgaca acaaggtctt gttgatacaa ttcctcaaag cggaaattat
      241 atctcattaa ttgatttaga ttcggccaaa aatgctcgat ttgtcagaga acatttggaa
      301 cgacaaatta tgttagagtg ttgtgccaaa atgaataacc aaaaaaaacg gattttagaa
      361 actataattt cagaacaaga aaaagctgtt agtcaaaaaa atgctcgtag tttttttcat
      421 aatgataatt tattccataa agcttgtttt gaaattgcag agcgagaaga agtatggcaa
      481 tggttagaaa ataacaatac acacttagaa cgatttcgat ggctacgagt aaaaacggaa
      541 ggattaaagt gggaaaaaat tatggaacaa cattatcaac tatttgaggc attaacaaaa
      601 caagatccag aagaagctaa ctttctaaca tcgttgcatc ttcatttaat gcttaatgag
      661 caaaatattg ttttagctac tcatccaaaa tatttcaaaa
//
LOCUS       DAYYGO010000281          698 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000281 DAYYGO010000000
VERSION     DAYYGO010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.295"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            41..277
                     /locus_tag="ACHA5T_002957"
     CDS             41..277
                     /locus_tag="ACHA5T_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8888436.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            274..672
                     /locus_tag="ACHA5T_002958"
     CDS             274..672
                     /locus_tag="ACHA5T_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFM8888437.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT          252 a          112 c          125 g          209 t
ORIGIN      
        1 acttactata gaatgagaat ggtatagagg gtggcgaaat atgaaaaaaa tgaaaaagta
       61 taaaattaga aaatctggca atagcgatgt aacaactatc ccaccagaag taaaagaatt
      121 catgggtgtg cagacaggtg acgcaatcag ttatgttttt caatctgatg gttctgtacg
      181 aatgatcaaa gcacaagaag aacctgatat tgattcatta gttgattcga tcatgaatca
      241 atatgaagac gcgttaaaag atttggttga tttatgaggt atctttcgac taaagaaatt
      301 atcaaaatca acgcgaaagt tattttacgt cactcgccag gcgaaatgat cggtgtaaaa
      361 gatgcaaatg cattagatat ggccgtcaaa cagccttcac aagcagtatt caatcaagaa
      421 ctctatcctg aaatctatga aaaagcggcg attttagcaa ttaatttagc taaaaaacat
      481 ccttttcata atggcaataa acgaactgct cttgttgcca tgttgttgtt tcttcaattg
      541 aataattgtc cagttgcttt ttctcgtcaa gaagctgttg attttatcat aatgattacc
      601 actagttcat tggatttcga ttcactaaag tccaagataa ccagttattt acgacaaacc
      661 gcaataaaat gaacgaatta aaaactttat ttctcaat
//
LOCUS       DAYYGO010000282          690 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000282 DAYYGO010000000
VERSION     DAYYGO010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.297"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(35..>690)
                     /locus_tag="ACHA5T_002959"
     CDS             complement(35..>690)
                     /locus_tag="ACHA5T_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8888438.1"
                     /translation="KDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWH
                     PWEDYRNFFVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFT
                     LIIEHETGIVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQ
                     LYDEVMHDNNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
BASE COUNT          237 a          121 c           78 g          254 t
ORIGIN      
        1 attgacccta acccaaaaaa aattactttc atcattaatt tacctcaatg tcgttaatta
       61 agttaataat atacttatct ttttcaaaag tatattgggg ttccattttc ttttccaaaa
      121 gcgtgaaaaa ttctttaatc tcctctaaat atgcatcttc aataatagta tgtgaataat
      181 tattatcatg cataacctca tcgtacaatt gaatatctgt catactagaa gtttcttcat
      241 tccatgaaaa tagagaatcc gggttacctt cccattctat taaaatcttt tcaccaatta
      301 tagttaaatt tcttttagct actcttgaaa caacatctac acttaaagtt ccaactatac
      361 cagtttcatg ctcaataata agagtgaaac tgtccgaata atcaatatca agattggata
      421 atttctgggt cattacagta aacgatttaa ctttcccaaa tgtatctata atccaaggta
      481 attcaatagc gaatatttct ctacacccat tagttttttt gtttgaaaca aaaaaattgc
      541 gataatcttc ccaaggatgc cagtcgggta aatactgtcc tacatggtat tgataagaaa
      601 tttttttatt atgcgtcact ttagacttaa tatattgaat ttcttttctc ttcaaaaatg
      661 tagaagatag ataaagtaat ctatctttct
//
LOCUS       DAYYGO010000283          684 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000283 DAYYGO010000000
VERSION     DAYYGO010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.299"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            94..>684
                     /locus_tag="ACHA5T_002960"
     CDS             94..>684
                     /locus_tag="ACHA5T_002960"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFM8888439.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSN"
BASE COUNT          266 a           86 c          118 g          214 t
ORIGIN      
        1 aaatatttct ttgaaaacgt agtaacaggc aaagaaaact ggaatgattt atatcatgca
       61 aacgaagtat tgaaaatagc tgaggagaaa taaatggaaa ttttatttac tatttgtggt
      121 agagctgggt ctaaggggtt taaaaataaa aatttgaaat atttggcagg aaagccccta
      181 gtccatttcg ctttagcaat gatagatatc tatcaacaaa aacatcctga acataattgc
      241 acgacttgtt tgaatacgga cagtaatgag ttagtagaat tgacgactaa gttaaatccg
      301 tctgtgaaaa ttgtagaacg taaacctgaa ttagctacag atactgtagg gaaaattgaa
      361 gtgatacgtg attcattctt acaactagaa agagaagata atatatttga tttaattata
      421 gatttagatt tgacttcacc tttacgtgaa gtttctaatc ttgaagaatt aatagatgaa
      481 catctaaaaa atttagataa agacgtctac ttttcagttg tttctgcaag acgtaatccc
      541 tattttaata tggtaaaaag aattgaaaat gaagttacta tagttaataa atcaagatac
      601 acatctaggc aacagacacc tgaagttttt gaaatgaatg cctcaatgta ttcttttaaa
      661 cctgaatttc taaaaaaaag taat
//
LOCUS       DAYYGO010000284          644 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000284 DAYYGO010000000
VERSION     DAYYGO010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.301"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>644)
                     /locus_tag="ACHA5T_002961"
     CDS             complement(<1..>644)
                     /locus_tag="ACHA5T_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888440.1"
                     /translation="KEAKNLCIFVLLLHGRVINLVNRKSTTKFANSFWFLENINETVE
                     KNGVYKGDFHFQNAETIEREHHYFDLLVNTVVELAKWAKSNETYSQVLKRIMSQETSF
                     IYELSKSNLKYTYNEDELEILDNPENSQWIKELKREDFIETLRILKSLFDLKLAKATI
                     MGIERDSYYWEEIGLYAPQIEGEDKEKRIDNIINYINSLSEPLKCRGLDFIGTLG"
BASE COUNT          202 a          119 c           65 g          258 t
ORIGIN      
        1 cctaatgttc ctataaaatc aagtcctcta cattttaaag gttcacttaa actattaata
       61 tagttaataa tgttgtctat ccttttctct ttatcttcac cttcaatctg gggagcatat
      121 agtccaattt cctcccaata atatgaatct ctttcaattc ccatgatggt agctttggct
      181 aatttcaaat caaataacga ttttagaatt cttagtgtct caataaagtc ttctctcttc
      241 aactctttaa tccattgtga attttctgga ttatctaaaa tttcaagttc atcttcatta
      301 taagtatatt tcaaatttga cttgcttaac tcatagataa atgaagtttc ttgactcata
      361 attcttttaa gtacttgact ataagtttcg tttgactttg cccatttcgc taattctacc
      421 actgtattca ctaataaatc gaaataatga tgttctcgtt caatagtctc agcattttga
      481 aaatgaaaat cacctttata aactccattc ttttccactg tttcattaat attttctaaa
      541 aaccagaatg agtttgcaaa tttagtcgta ctttttcgat taactaagtt aataactctt
      601 ccatgtaata ataagacaaa aatacataaa tttttagctt cctt
//
LOCUS       DAYYGO010000285          588 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000285 DAYYGO010000000
VERSION     DAYYGO010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.302"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(65..150)
                     /locus_tag="ACHA5T_002962"
     tRNA            complement(65..150)
                     /locus_tag="ACHA5T_002962"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Leu,seq:taa)
     gene            complement(167..238)
                     /locus_tag="ACHA5T_002963"
     tRNA            complement(167..238)
                     /locus_tag="ACHA5T_002963"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(204..206),aa:Gly,seq:gcc)
     gene            complement(250..322)
                     /locus_tag="ACHA5T_002964"
     tRNA            complement(250..322)
                     /locus_tag="ACHA5T_002964"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(287..289),aa:Thr,seq:tgt)
     gene            complement(340..421)
                     /locus_tag="ACHA5T_002965"
     tRNA            complement(340..421)
                     /locus_tag="ACHA5T_002965"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(385..387),aa:Leu,seq:tag)
     gene            complement(429..501)
                     /locus_tag="ACHA5T_002966"
     tRNA            complement(429..501)
                     /locus_tag="ACHA5T_002966"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(466..468),aa:Lys,seq:ttt)
     gene            complement(505..577)
                     /locus_tag="ACHA5T_002967"
     tRNA            complement(505..577)
                     /locus_tag="ACHA5T_002967"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(542..544),aa:Val,seq:tac)
BASE COUNT          149 a          169 c          138 g          132 t
ORIGIN      
        1 cccctaacct tttgatccgt agtcaaacac tctatccagt tgagctatgg gcgcatattt
       61 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
      121 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      181 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      241 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      301 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      361 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      421 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      481 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      541 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DAYYGO010000286          576 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.303, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000286 DAYYGO010000000
VERSION     DAYYGO010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.303"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            80..>576
                     /locus_tag="ACHA5T_002968"
     CDS             80..>576
                     /locus_tag="ACHA5T_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFM8888441.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
BASE COUNT          217 a          106 c           93 g          160 t
ORIGIN      
        1 aaaaatccat acaaaaaagg aagtcccttc tgtagaataa agttaacgac aaccaattca
       61 cagaaaagag gacttcccta tgaatgattt tactacagaa attgtgcaaa ctctagtcac
      121 taaaggcgat ttaaatgaat tattccgttc gcacttagaa aaagcgataa acacactcct
      181 acggactgaa ttaacggctt ttttagatta cgaaaaatat gatcgcactg gttttaattc
      241 aggtaattcg agaaacggtt cttactttcg atcaatcaaa accgaatatg gtgaattaac
      301 attggaaata cctagagatc gtaatggtga gtttaaacaa caaactttac cagcctacaa
      361 aagaacaaac gatacattgg aaaccactat tatccattta ttcgaaaaag gtgttacgat
      421 gtctgaaatt gctgatttga tcgaaaaaat gtacggtcat cactatactc cacaaaccat
      481 gtccaacatg actaaagttc tgactgaaga agtaaatgcc tttaaatcca gagccttaaa
      541 tgataagtat gtcgctattt ttatggacgc tactta
//
LOCUS       DAYYGO010000287          575 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.304, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000287 DAYYGO010000000
VERSION     DAYYGO010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.304"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            258..575
                     /locus_tag="ACHA5T_002969"
                     /pseudo
     CDS             258..575
                     /locus_tag="ACHA5T_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT          195 a          105 c           82 g          193 t
ORIGIN      
        1 ttttacactc tctattattc ttttcataaa tattgtaaat agaagtatta aaaatatata
       61 caaaaagtat tatttcgaga aagataaata ttttcaaaaa ccttttcata atcctcaccc
      121 tttcttacac tgataaatca tgcgtctaat attcaaatta tactcgatat gctttagcta
      181 atcagccaac tctgtttgtc tactatcatt atatcaatag acaatatctg ctcccgtctt
      241 tatgatcgtg ccaaagcaat caaaagaaaa ttgcgaatca cttagatcga aagtttactc
      301 aaacaaaagc attgtcagcg gttgtcacgg atttaactta tgttcgggtc gggacaaatt
      361 gggcttatat ttgtttgatg attgatttat tcaatcgtga aattattggc cattcttgcg
      421 gaaataaaaa agatgcgcag cttgtaaaaa gggccattca aagcattcct tactcacttc
      481 aagaaatcga actcttccat acggatcgtg gaaaagaatt tgataaccaa accattcaga
      541 atctcatgaa tggtttggtt atcaaattcc tttcc
//
LOCUS       DAYYGO010000288          569 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.305, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000288 DAYYGO010000000
VERSION     DAYYGO010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.305"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>569
                     /locus_tag="ACHA5T_002970"
     rRNA            <1..>569
                     /locus_tag="ACHA5T_002970"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          139 a          131 c          173 g          126 t
ORIGIN      
        1 gagagtggaa ttccatgtgt agcggtgaaa tgcgtagata tatggaggaa caccagtggc
       61 gaaggcggct ctctggtctg taactgacgc tgaggctcga aagcgtgggg agcaaacagg
      121 attagatacc ctggtagtcc acgccgtaaa cgatgagtgc taagtgttgg agggtttccg
      181 cccttcagtg ctgcagctaa cgcattaagc actccgcctg gggagtacga ccgcaaggtt
      241 gaaactcaaa ggaattgacg ggggcccgca caagcggtgg agcatgtggt ttaattcgaa
      301 gcaacgcgaa gaaccttacc aggtcttgac atcctttgac cactctagag atagagcttc
      361 cccttcgggg gcaaagtgac aggtggtgca tggttgtcgt cagctcgtgt cgtgagatgt
      421 tgggttaagt cccgcaacga gcgcaaccct tattgttagt tgccatcatt cagttgggca
      481 ctctagcaag actgccggtg acaaaccgga ggaaggtggg gatgacgtca aatcatcatg
      541 ccccttatga cctgggctac acacgtgct
//
LOCUS       DAYYGO010000289          568 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.306, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000289 DAYYGO010000000
VERSION     DAYYGO010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.306"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            145..543
                     /gene="spxA"
                     /locus_tag="ACHA5T_002971"
     CDS             145..543
                     /gene="spxA"
                     /locus_tag="ACHA5T_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFM8888442.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          200 a           88 c          111 g          169 t
ORIGIN      
        1 tagataactt tcattttttt aaaaaaatta tgaaaaaaag gtagctattt aaaagttgat
       61 tatgtataat agaaacagat gggagattat tatgatcttc tgttacaaaa aagtgattct
      121 tatcacgaaa aggagtggtg cacgttgtta acattatata cttccccaag ttgtacttcc
      181 tgcagaaagg ctcgtgcgtg gttgcaagaa catcagattc catttgttga acgtaatatt
      241 ttttcagaac cattgaacag ttctgaatta aaagcgattt tacaaatgac agaagacgga
      301 acagaagaaa tcatctcgac acgttccaaa gtattccaaa aactgaacat ggatttagat
      361 gatctgccgt tacaagaatt attggaactt gtgcaaaaca atcctggatt gcttcgccgt
      421 ccaatcatga ttgatgataa acgtctgcaa gttggtttta atgaagatga aattcgtcgg
      481 ttcctaccgc gcgatgtacg acaattagaa ttacgtcaag cacaattgat ggctggatta
      541 tagaaggata atacgaaact tggaaaag
//
LOCUS       DAYYGO010000290          559 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.307, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000290 DAYYGO010000000
VERSION     DAYYGO010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.307"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            168..362
                     /locus_tag="ACHA5T_002972"
     CDS             168..362
                     /locus_tag="ACHA5T_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888443.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
BASE COUNT          236 a           70 c           89 g          164 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcat
//
LOCUS       DAYYGO010000291          534 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.308, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000291 DAYYGO010000000
VERSION     DAYYGO010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.308"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>534
                     /locus_tag="ACHA5T_002973"
     CDS             <1..>534
                     /locus_tag="ACHA5T_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFM8888444.1"
                     /translation="DGFKACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDL
                     KPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNT
                     SAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINWARGGQVGSWFADGTYGHTGVIR
                     GLANGRMQTYEQNTELG"
BASE COUNT          163 a           80 c          137 g          154 t
ORIGIN      
        1 tgatggattt aaggcctgta ctgatccaag acaagcagca tatgcttttg aacgaaacta
       61 tgaacgtccg gcagcgacac atcctgaacg tcaagattat gcagttaact ggtataacaa
      121 atttaaagat ttaaaaccag gaggagctac tggagaagca ggactaaaac atttagagtc
      181 tttgattgga caacgaattg gcaatggtca gtgttatggc ttgtctgcag aatattcagg
      241 atatctgggt ggttgtggca tgggtgctgg aacaaaatat ggtttaactc atgtgatagg
      301 aaatacttct gcagcatctg atattggtat tgcctatgat tggtctgctg ttggttggaa
      361 agtgattcaa aaccctagat atgatcaatt agtagttggt gcaattatta attgggcaag
      421 aggtggacaa gtgggttcat ggtttgcaga tggaacttat ggacatactg gcgttatccg
      481 aggcttagct aatggtcgta tgcaaactta tgaacaaaac acagagttag gtat
//
LOCUS       DAYYGO010000292          514 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.310, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000292 DAYYGO010000000
VERSION     DAYYGO010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.310"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..132)
                     /locus_tag="ACHA5T_002974"
     CDS             complement(<1..132)
                     /locus_tag="ACHA5T_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFM8888445.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          186 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaa
//
LOCUS       DAYYGO010000293          507 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.311, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000293 DAYYGO010000000
VERSION     DAYYGO010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.311"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            117..>507
                     /locus_tag="ACHA5T_002975"
     CDS             117..>507
                     /locus_tag="ACHA5T_002975"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8888446.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFS"
BASE COUNT          195 a           58 c           89 g          165 t
ORIGIN      
        1 tttcctattc ttaaaatgat gtttgtactt acttaaatta ggtataatta gtatatgttt
       61 tgtgagaaat ttagacttac aataagcagt gaatttataa tcaacgtata ggagacatgg
      121 atgaaatgaa actaattggt atagatttag acggtactct tttgaattct gaccagagca
      181 ttagtcaaaa aaatgcaaga acaatgaaac atttaccgga agactgtttt ccattcattt
      241 gttctggaag agaagtagaa gacattaata atattctcaa aaaatctggt ttaaggatac
      301 cagcagtagg gttaaatggt tctgttggat ttgatggaga aaaaaagata tttgagtctt
      361 ccttcgataa aaatgatgta aaaaaaattg attctgtcct atcgaaattt ccaaccaaaa
      421 tatatacaaa tctcggaagt tatgaatctc agaattataa aaataaaatg aaaaaaattt
      481 ttcacgaggt agggagtgaa ttttcta
//
LOCUS       DAYYGO010000294          487 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.312, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000294 DAYYGO010000000
VERSION     DAYYGO010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.312"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>487)
                     /locus_tag="ACHA5T_002976"
     CDS             complement(<1..>487)
                     /locus_tag="ACHA5T_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8888447.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IK"
BASE COUNT          142 a           92 c           86 g          167 t
ORIGIN      
        1 gttttatccg ttcattattg tagtaataga tatagctatc gatggctgat tttaattcct
       61 cgaaacagtt ataaactttc ccgtgaaaaa tctcttgttt taataagcca aaaaaattct
      121 ccataagtga attatctaag cagttccctt tccgagacat gctttgaaag attttgtact
      181 ccttcaattt gtttctataa gctttcattt gatacgccca accttgatct gagtgaaacg
      241 tacgacgata gggacaatcg ttagtggtct ggatagcttc ctccaaacct tccattaccg
      301 ctaatgcatt gggcttttca gagatacgat aggataaaat ctcactattg aacatatcca
      361 taaaaggatc tagatacaat ttcttttgac ggataatgcc tttggcatcc acttcatggt
      421 atttaaattc agttgtatcc gtcgtgattt tctgatggca gatactggtg tagaaacgac
      481 gatggat
//
LOCUS       DAYYGO010000295          477 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.313, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000295 DAYYGO010000000
VERSION     DAYYGO010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.313"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          169 a           66 c           79 g          163 t
ORIGIN      
        1 tgaggctgtg acaaatgtca ttttttgttt cgctaaaaac gagtgaaaaa atgttgataa
       61 ctccaatcat tcgtttaaga ttctaggaat cattcaggaa tgctgtgaaa ccgatacttt
      121 ataattataa agaaggtgat ttcaaattcc gaagaaggaa tttgaagtca cctttttact
      181 tatgtcacag tctcattcag taatgcggga taaggctcct tattccgcta tttgaatact
      241 ctgaagttta atctaagaaa gagtaatatt ttgaaaacat gcttcatcgc atagaaatga
      301 acttaatttc agaaagaata catacatatc tttttatatg ttttcagctg ctgaatggtt
      361 aaaaagcaca aatatccgaa aaaaacatga agaaatgtac tttttttgtt gtaatgtgca
      421 tgaaattctt taagtaaact agttaacata agtttagtaa gattcctatt ctgtgaa
//
LOCUS       DAYYGO010000296          476 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.314, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000296 DAYYGO010000000
VERSION     DAYYGO010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.314"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            280..>476
                     /locus_tag="ACHA5T_002977"
     CDS             280..>476
                     /locus_tag="ACHA5T_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8888448.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTL"
BASE COUNT          173 a           76 c           68 g          159 t
ORIGIN      
        1 aggttttaga aaagatttat tatacgtaaa aagctcaaga cattttctta gccataagga
       61 catgtttgat atttaagaag atatatgaat tttcttttct aagtataaag ataactatcc
      121 attatcaact aaaaaaacat ttttgaaaat aaacatcagc agtcctatca atgcatgtga
      181 gcttatagaa gttattcgca gcgcagcata attgcattag cttcttttcg tgttttttgc
      241 ctcttttccc tttaaatctt aatggaggag gtgtgcaata tgtcagataa cgacgaacgc
      301 gtaaatgccc agttgattcg attctttgaa aaggtcattt tgaacaccgc taatacttat
      361 tataaaaaac aagaagaaat tcaaaaacat gaacaacttg atcaactccc accatatttg
      421 tttactaaag atactataaa atttaaacaa ccgattaatg ttttaaatgt gactct
//
LOCUS       DAYYGO010000297          448 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.315, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000297 DAYYGO010000000
VERSION     DAYYGO010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.315"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..>448
                     /locus_tag="ACHA5T_002978"
     CDS             22..>448
                     /locus_tag="ACHA5T_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8888449.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYIEKRSTEGWTPDVIIGRAERTFS
                     CSVSTLYRRFKTGEFNVLHLPMQGKRKPNGYKEKRGKQAF"
BASE COUNT          162 a           85 c           91 g          110 t
ORIGIN      
        1 atacaaggag agtgaacata aatgacttat acccatctta caccaaacga gcttgtaatg
       61 atagaagcat attttcatca agaaactccg gttgctatcg ttgcgaagca gcttaaacgt
      121 ggacgccaaa caatttacaa tgtctataac tttctcaaat gtggtggaac agcacttgaa
      181 tactttgaac aatacaaaga aaataagcga cgttgtggga gaaccgaaat catttttcct
      241 gctgaggaaa aagaatacat tgaaaaaaga tcaactgaag gttggacccc agacgtcatt
      301 attggccgtg cagagcgaac cttctcttgt tcagtaagta ccctctatcg ccggtttaag
      361 acgggagaat tcaatgtttt acatttaccg atgcaaggaa aacgaaaacc aaatggttat
      421 aaggaaaaac gtggaaaaca ggcattca
//
LOCUS       DAYYGO010000298          444 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.316, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000298 DAYYGO010000000
VERSION     DAYYGO010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.316"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          207 a           56 c           68 g          113 t
ORIGIN      
        1 aattccttag gcagcttttg ctgtgtagaa aggaataaat aataacgaaa gttcaaaagc
       61 taatgccgac tattctagac attaaagaag acgaaaacga acgataaaga aagaaaagtt
      121 atgaataatc gtttattgaa aaaaacttga aaaaatacaa aaaattcgtt gaccaagcta
      181 aatttagttg gtatattatt acttgtccaa aagacagaag aaaatattta ttcaaaaaca
      241 taacaaaaaa gttattgaca tgttactaac gacatgataa gataactgag tcgttaaaaa
      301 agaaccactt gcgaagcttg attctatcga aaaaaacttc aaaaaagttc ttgacaaata
      361 acaaacgata agttatgata aatgagttgc cgaaagattt cgaaaaacga aatcgaaaaa
      421 aacttttaaa aaagagttga caat
//
LOCUS       DAYYGO010000299          432 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.317, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000299 DAYYGO010000000
VERSION     DAYYGO010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.317"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>432)
                     /locus_tag="ACHA5T_002979"
     CDS             complement(<1..>432)
                     /locus_tag="ACHA5T_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888450.1"
                     /translation="ADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNIL
                     LALVRYVNWEGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRK
                     AFPLGLKEEDKKSAETKPLFQLVCQGLEEQKGILDLQEEDV"
BASE COUNT          124 a           89 c           54 g          165 t
ORIGIN      
        1 agacatcttc ttcttgtaag tctagaattc ctttttgctc ttccaatcct tgacagacta
       61 attgaaataa cggcttcgtt tctgcgcttt tcttatcttc ttctttcagt ccaaggggaa
      121 atgcctttct tccgattgcg atctctattc ctcgtatgat aaaggacttt tcatcttccg
      181 aatatcttct tttaaaaaaa tgagtctcta cataatcaat caactcgtta caagcttctt
      241 ctacatacgg atttttattt tcatagcctt cccaattgac ataacgtact aaagctagca
      301 agatgttccg cgagtcaaat tctgggataa tgatttcttt atttttcaaa taatcatttt
      361 ctttaaaaaa atccaatacg atttttttga ttgtatatac tttgctcaaa gaaataaatt
      421 caagatcagc ca
//
LOCUS       DAYYGO010000300          402 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.319, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000300 DAYYGO010000000
VERSION     DAYYGO010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.319"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>402
                     /locus_tag="ACHA5T_002980"
     CDS             <1..>402
                     /locus_tag="ACHA5T_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HFM8888451.1"
                     /translation="VLFPNGDFPSRSRFTRLSSNLAYTIKIIRYFFIKKLTKGELVGI
                     IDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLKIHMIVTKTGFPITYSITN
                     PGVHDVKVLETLSEEANLPNILGDKGYISHK"
BASE COUNT          137 a           76 c           66 g          123 t
ORIGIN      
        1 ccgtcttatt tcctaatggt gactttccta gtaggagtcg attcactcgc ttaagttcaa
       61 acttagctta cactatcaag attattcgat actttttcat caagaagctc accaaaggtg
      121 aactagtcgg aattatagat agttttccta gtccattatg taaaccagtt agaaatagac
      181 aagcaaaact attgaatcaa atagccaaag ttggctataa ctcaacaaaa aaatcttatt
      241 tttatggtct taaaattcat atgatcgtta ctaagactgg ctttccgatt acttactcaa
      301 tcacgaatcc aggtgtccat gacgtgaaag tgttagagac tttatcggaa gaagcgaatc
      361 ttcccaatat cctaggggac aaaggatata ttagccataa aa
//
LOCUS       DAYYGO010000301          401 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.320, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000301 DAYYGO010000000
VERSION     DAYYGO010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.320"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..117
                     /locus_tag="ACHA5T_002981"
     CDS             <1..117
                     /locus_tag="ACHA5T_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8888452.1"
                     /translation="KFIFNKTDKEIGKVLGITRQGATNLKHRLYKKIQKMLV"
     gene            215..>401
                     /locus_tag="ACHA5T_002982"
     CDS             215..>401
                     /locus_tag="ACHA5T_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8888453.1"
                     /translation="MNTLKEDFILAKNGNEEAVVAILKRFSALVHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIK"
BASE COUNT          154 a           56 c           68 g          123 t
ORIGIN      
        1 aaatttatct ttaataaaac tgataaagaa attggaaagg ttctgggtat tacaagacag
       61 ggtgcgacaa atttaaaaca tcgactttac aaaaaaattc aaaaaatgct ggtttaatat
      121 ctgcataata actaattttg tatgcttctt tgctcgtcct cctccttccc caaaaaataa
      181 atagattata agttcatggg aagaaggtaa atttatgaat actttaaaag aagatttcat
      241 tttagcaaag aatggaaatg aagaagcagt tgtggccatt ctcaaaagat ttagcgcttt
      301 ggtccataag caatcctgga gaactggaaa atatgatcaa gattgttatc aagaatgtat
      361 gcttgcaata tatctagcta tctcgaaatt cgaaattaaa g
//
LOCUS       DAYYGO010000302          398 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.321, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000302 DAYYGO010000000
VERSION     DAYYGO010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.321"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          116 a           72 c           53 g          157 t
ORIGIN      
        1 gtttaagaaa gtttaaacat tgcgcaattt aattatttta gctgtttctt ttcataaaaa
       61 aacatgttgt gactctgaga caaatcatag tccatagtga tgtctctaga gtaactacgc
      121 ctaatattac gaggcatcat attaatctat cagcactagt acctaaacta gcatcccttt
      181 cgaatatttt ttcttatata ataaagtttg ctttcactcc tctaatctca ccaaatcgac
      241 tggcttgcgt tgtgtgatac taggaactga ttggtttaga acgacttttc tattgtaatc
      301 atatttttcg ccatcctcgt tgtcttctaa tccttcaatg aatgatccct aatgtgtgta
      361 ttaaataatt taattttttt gttcctattt atttaaca
//
LOCUS       DAYYGO010000303          394 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.322, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000303 DAYYGO010000000
VERSION     DAYYGO010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.322"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            181..330
                     /locus_tag="ACHA5T_002983"
     CDS             181..330
                     /locus_tag="ACHA5T_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888454.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
BASE COUNT          127 a           64 c           67 g          136 t
ORIGIN      
        1 ttgaataacc gcgattgatt cactttttgt tttcttgttt atttgctttt atctgcaact
       61 tttgacttta aaatttctta actatgaaaa cctttcaatt ggtggtatac ttataaatgg
      121 aatttcgtta tcaacgcgtt cgttgccttt cagcaagtat ttaaagaaag gagaatggct
      181 atgacattct ataacaaaga tatacaatat cgagtaacct tggatacaga tttgaatcta
      241 tttgtcgttt ttgacaaaga aaataccaat catgtagcga caggtgttac gatcgaacaa
      301 gcagtgcaag aattaaaaaa atctgcataa ggagtggaaa ccctcatatg gacgattctc
      361 gttcttctct ttaaacgcat ctactggtag ataa
//
LOCUS       DAYYGO010000304          369 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.325, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000304 DAYYGO010000000
VERSION     DAYYGO010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.325"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..261
                     /locus_tag="ACHA5T_002984"
     CDS             <1..261
                     /locus_tag="ACHA5T_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFM8888455.1"
                     /translation="LSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIYGIEAEA
                     NMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEHLVLQH"
BASE COUNT          131 a           55 c           59 g          124 t
ORIGIN      
        1 ctatcacctt ctatgaaaag atttgtatta tggcatgaaa tagcccacgt tagactccat
       61 aaaggtattt gcacagcagc ttttagagca aacaacctta gttgtatgat ttatggtata
      121 gaagctgaag ctaatatgtt tgcaattgaa atgttaacta gaacaataga tgaacaagat
      181 ttatctgata tgagtcagtt ccaaattatt gaatatttag ggcttcctca taatcttgaa
      241 catttagtat tacaacatta aaaataaaaa agcccatgct ttcacatgga ctctttcctc
      301 atttctgagt aaactctaca ataattatat caaagaaatg aggagttttg aatgttatta
      361 ttgattgga
//
LOCUS       DAYYGO010000305          368 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.326, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000305 DAYYGO010000000
VERSION     DAYYGO010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.326"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..272)
                     /locus_tag="ACHA5T_002985"
     CDS             complement(<1..272)
                     /locus_tag="ACHA5T_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888456.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKT"
BASE COUNT          106 a           69 c           52 g          141 t
ORIGIN      
        1 aaagttttga tttgttgcgt gtgttccacc aatttttctg tatgtgtatc taatctttca
       61 tcgtgtttct ttagttcttc atgaatcatc aatcgatctg atttgctcga ttctaaatct
      121 ttagtcagca aatctaaatt gtggcttact tttgaaagag tctcagtaat tttcgagaaa
      181 gatgcagcaa tcggtctaat tactaataaa atcaaagaaa cgatagcggt tattgatcct
      241 gctatcattc cccattcccc taaattaatc atgtgacaac tcctttacct taaataaaaa
      301 cgcatcaatt aagatgcgct cttatcttta ttaatgattt tatctgcttc ttcgtctgta
      361 atgcataa
//
LOCUS       DAYYGO010000306          364 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.327, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000306 DAYYGO010000000
VERSION     DAYYGO010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.327"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>364
                     /locus_tag="ACHA5T_002986"
     CDS             <1..>364
                     /locus_tag="ACHA5T_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFM8888457.1"
                     /translation="EDIFAYILEQAVKAGFVTEDNLYLDSTHIKANANKHKFTKEMTH
                     DEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPESGYYVKGEREK
                     QFAYSAHTSCDDNGFILST"
BASE COUNT          137 a           62 c           76 g           89 t
ORIGIN      
        1 tgaggatatt tttgcctata ttttagaaca agcagttaaa gcaggttttg taacagagga
       61 taatttgtat ctggattcta cccacatcaa agctaacgcc aataaacata agtttacgaa
      121 agaaatgacc catgatgaag cgaaagccta tcaggatgaa ttagaagatg agatcaatcg
      181 tcaacgaata gaggctggaa aacgcccttt tactttggac ttagaaaagg aagtgaagct
      241 gaaagaaaga aaaataagta aagctgatcc ggaaagcggc tactacgtca aaggcgaacg
      301 agaaaaacaa tttgcttatt ctgcccatac ttcatgcgac gacaatggat ttattcttag
      361 caca
//
LOCUS       DAYYGO010000307          360 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.328, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000307 DAYYGO010000000
VERSION     DAYYGO010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.328"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>360
                     /locus_tag="ACHA5T_002987"
     CDS             <1..>360
                     /locus_tag="ACHA5T_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8888458.1"
                     /translation="DRRLYKLTQYFLRFPRKARLKVKYLVMDMNASYCQLLKTVFPNA
                     EIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYRRLKRYWKLLLKDSTTLEP
                     LKRHYHRLFKRPISQTE"
BASE COUNT          117 a           88 c           62 g           93 t
ORIGIN      
        1 aagatcgcag actttacaaa ctaactcaat acttcctgcg ctttcctaga aaggcccgtt
       61 taaaggtcaa gtacctagtg atggacatga atgccagcta ttgtcagttg cttaaaacag
      121 tctttccaaa tgcggagatc gtaactgacc gctttcatat tgtccaacac atcaatcgca
      181 gcttcaatca attacgtgtc cagatcatga atcgttttcg aacctcacac tctgaaaatc
      241 agaagaagta ccgtcggctc aagcgatatt ggaaactctt actcaaagat tccacgacat
      301 tggagccact caaacggcat tatcatcgac tgtttaaacg accaattagt caaacagaaa
//
LOCUS       DAYYGO010000308          325 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.330, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000308 DAYYGO010000000
VERSION     DAYYGO010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.330"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>325
                     /locus_tag="ACHA5T_002988"
     CDS             <1..>325
                     /locus_tag="ACHA5T_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFM8888459.1"
                     /translation="KQGFTDIINGQTPQTPVVEESTQQATTTTTQSTSSSGISNWEAA
                     KETVLNNKALWIYESPDKNEANTNIIESTQYRKDLMQDENGEFYPVDISTDDIGNTVM
                     NGGKQ"
BASE COUNT          140 a           49 c           53 g           83 t
ORIGIN      
        1 taaaacaagg atttacagat atcattaatg gacaaactcc ccaaacacca gtagtagaag
       61 aatctactca acaagcaaca actacaacga cacaatctac aagtagttca ggaatttcaa
      121 attgggaagc tgctaaagaa actgtactaa ataataaagc gttatggatt tatgagtctc
      181 cagataaaaa tgaagcaaat actaatatta ttgaatccac ccaatataga aaagatttaa
      241 tgcaagatga aaatggagaa ttttatccag ttgatatttc tactgatgat attggaaata
      301 cggtaatgaa tggaggaaaa caatt
//
LOCUS       DAYYGO010000309          316 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.332, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000309 DAYYGO010000000
VERSION     DAYYGO010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.332"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            15..>316
                     /locus_tag="ACHA5T_002989"
     CDS             15..>316
                     /locus_tag="ACHA5T_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFM8888460.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYV"
BASE COUNT          112 a           34 c           60 g          110 t
ORIGIN      
        1 gaaggagtat aaaaatgaac aaacataata gaaaaataac gaataacaaa gaactgatct
       61 tgttactaat agggatttgg ggtatctttt tactctttaa tatgtatatg ccgactatga
      121 gagcggatga cgtagtttat gcaagtagat tagatgaact tggctatttg ggtgcttcga
      181 tagaacatta taagacatgg agttcaagaa ttattataga actattctta atgttctttt
      241 ctaaacactt tatgctatgg aaattattaa attctacgat tatgttagga attgtagtat
      301 tactttgcaa gtatgt
//
LOCUS       DAYYGO010000310          302 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.334, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000310 DAYYGO010000000
VERSION     DAYYGO010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.334"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          121 a           42 c           49 g           90 t
ORIGIN      
        1 ttttttcaaa aatatggtat gcttcttagt gaattcaaat ataaaagagt ttaaagcgta
       61 atacacttat ggggaagtgg tttggggtgc gctttaaact cttctttatt attatctcac
      121 agcatgctta aaaggtctat ggattaagtt atgtaaaaaa gtgcctcttt aaacgtccta
      181 aaatccaaac aaggatagca taatgtacac tatcgttgat ccagaaaaca ggtaaataaa
      241 ttttgcaaaa gcaataagtt aaaaacatta caaaaaagac aaggatatca catcaagaaa
      301 aa
//
LOCUS       DAYYGO010000311          281 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.339, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000311 DAYYGO010000000
VERSION     DAYYGO010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.339"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>281
                     /locus_tag="ACHA5T_002990"
     CDS             <1..>281
                     /locus_tag="ACHA5T_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HFM8888461.1"
                     /translation="VGYYLRYNLSYREVQEILYDRGINVSHTTIYRWVQEYGKLLYQI
                     WKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWLRKKRDT"
BASE COUNT          100 a           43 c           54 g           84 t
ORIGIN      
        1 gtgggctact atcttcgtta taaccttagc tatcgtgaag ttcaagaaat cttatatgat
       61 cgtggcatta acgtttctca tacgacgatt tatcgttggg tgcaagaata tggcaaacta
      121 ctctatcaaa tttggaaaaa gaaaaataaa aaatcctttt attcatggaa aatggatgaa
      181 acgtacatca aaattaaagg aaaatggcat tatttgtatc gagccatcga tgcagatggt
      241 ttaaccttgg atatttggtt acgtaaaaaa cgggacacac a
//
LOCUS       DAYYGO010000312          275 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.340, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000312 DAYYGO010000000
VERSION     DAYYGO010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.340"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>275
                     /locus_tag="ACHA5T_002991"
     CDS             <1..>275
                     /locus_tag="ACHA5T_002991"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888462.1"
                     /translation="FIKIIRSLSLQGYTPISGWIFENDILSQELPFLHEDTTVVLSKN
                     DIRINLVYNNSMEKEYFSNTLDIPVKTSSKRNKPDIRLDIILQDKYY"
BASE COUNT          104 a           46 c           33 g           92 t
ORIGIN      
        1 tttataaaaa ttattagatc tctatccctg caaggctaca ctccaatttc tggatggatt
       61 tttgagaatg atattctttc acaagaattg ccgtttttac atgaagacac taccgtagta
      121 ttgagtaaaa atgatatacg tattaatctt gtatataata attccatgga aaaagaatat
      181 ttctccaata cactagatat cccagtaaaa acctcttcaa aacgaaataa acctgatatt
      241 agattagaca ttatccttca agataaatac tatat
//
LOCUS       DAYYGO010000313          267 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.341, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000313 DAYYGO010000000
VERSION     DAYYGO010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..267
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.341"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(35..>267)
                     /locus_tag="ACHA5T_002992"
     CDS             complement(35..>267)
                     /locus_tag="ACHA5T_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8888463.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
BASE COUNT           78 a           39 c           47 g          103 t
ORIGIN      
        1 tagaaggact gaccttctgt ctcagatttt tttgttattt cacttgaatc aattgattga
       61 tcaaaaaaat cctgattttc atgttctcaa atgatttaaa tccataagat aatcgtttca
      121 gtgtcttgat atggggaatc ttggcctcta ttttgccatt agaatacggg taaatcatag
      181 cattgtaaat accttcttca tacgttaaaa ggttttgaag tttcgtgcga agttgtgcat
      241 caagtgtttc aggaagattt tttaata
//
LOCUS       DAYYGO010000314          261 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.342, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000314 DAYYGO010000000
VERSION     DAYYGO010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.342"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>261
                     /locus_tag="ACHA5T_002993"
     CDS             <1..>261
                     /locus_tag="ACHA5T_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888464.1"
                     /translation="PGQAATITIGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPG
                     INATTTAVATTTANGLMSSTDKTKLDGIAAGAQKNPGNATTTT"
BASE COUNT           87 a           54 c           56 g           64 t
ORIGIN      
        1 tcctggccaa gcagcaacaa ttaccatagg aaccgtttct agtggttcaa ccgcttctgt
       61 tacgaatgta ggaacatcat ctgcagctag atttaatttt gttttaccaa agggagataa
      121 aggtgatcct ggaataaatg ccacaactac agctgtagct acaactacag caaatgggtt
      181 gatgtcgtcc acagataaaa ctaagttgga tggaattgca gctggagcac agaaaaatcc
      241 aggtaatgct acaactacga c
//
LOCUS       DAYYGO010000315          253 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.344, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000315 DAYYGO010000000
VERSION     DAYYGO010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.344"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>253
                     /locus_tag="ACHA5T_002994"
     CDS             <1..>253
                     /locus_tag="ACHA5T_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFM8888465.1"
                     /translation="GFTKQSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSY
                     PLVGPATWDGKVYVQNLFNAAGIKEPITSLDAQVRLLIWT"
BASE COUNT           80 a           40 c           58 g           75 t
ORIGIN      
        1 ggatttacga aacaatctgc atgtggaata ttggggaaca ttcaacaaga aacaggagga
       61 acttttgatc ctgatactgt tcaaataggt ggaccagcat acggattagt tcagtgggat
      121 ggttcttcat atcctcttgt tggtccagca acatgggatg gaaaagttta tgttcaaaac
      181 ttatttaatg ctgcaggtat taaagaacca ataacgagct tagatgcaca agttcgattg
      241 cttatttgga cat
//
LOCUS       DAYYGO010000316          252 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.denovo.345, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000316 DAYYGO010000000
VERSION     DAYYGO010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.denovo.345"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(32..>252)
                     /locus_tag="ACHA5T_002995"
     CDS             complement(32..>252)
                     /locus_tag="ACHA5T_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFM8888466.1"
                     /translation="NKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIITGF
                     GAFLGSVLGVSNRTYKMFSAESEEGGNK"
BASE COUNT           73 a           61 c           41 g           77 t
ORIGIN      
        1 aggctcatcg cagtaatagt aattttcttt ttcatttgtt tcctccttct tcgctttcag
       61 ccgagaacat tttgtaggtt cgatttgata cacccaacac actccctaaa aacgcgccaa
      121 aaccagtaat gatgacaaca cagatatctg tgtactgcca attgagcgct ttaccaacta
      181 accccacgaa agtagctagt gcgggaataa ttaccagtgc gaaccatttt agtacttcga
      241 acgttttatt at
//
LOCUS       DAYYGO010000317        21675 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.guided.044, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000317 DAYYGO010000000
VERSION     DAYYGO010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.guided.044"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            26..889
                     /locus_tag="ACHA5T_002996"
     CDS             26..889
                     /locus_tag="ACHA5T_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB6/TrbL-like conjugal transfer protein,
                     CD1112 family"
                     /protein_id="HFM8888467.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            926..1462
                     /locus_tag="ACHA5T_002997"
     CDS             926..1462
                     /locus_tag="ACHA5T_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HFM8888468.1"
                     /translation="MSTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVERL
                     ASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTRGN
                     AEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFARQK
                     TPGWDVWGNEVECDLILT"
     gene            1476..1898
                     /locus_tag="ACHA5T_002998"
     CDS             1476..1898
                     /locus_tag="ACHA5T_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HFM8888469.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            1828..4227
                     /locus_tag="ACHA5T_002999"
     CDS             1828..4227
                     /locus_tag="ACHA5T_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4-like conjugal transfer ATPase, CD1110
                     family"
                     /protein_id="HFM8888470.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            4259..6250
                     /locus_tag="ACHA5T_003000"
     CDS             4259..6250
                     /locus_tag="ACHA5T_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFM8888471.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            6273..6524
                     /locus_tag="ACHA5T_003001"
     CDS             6273..6524
                     /locus_tag="ACHA5T_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HFM8888472.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            6514..7743
                     /locus_tag="ACHA5T_003002"
     CDS             6514..7743
                     /locus_tag="ACHA5T_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1107 family mobile element protein"
                     /protein_id="HFM8888473.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            7740..9821
                     /locus_tag="ACHA5T_003003"
     CDS             7740..9821
                     /locus_tag="ACHA5T_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFM8888474.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            9970..13890
                     /locus_tag="ACHA5T_003004"
     CDS             9970..13890
                     /locus_tag="ACHA5T_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YodL domain-containing protein"
                     /protein_id="HFM8888475.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKID
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLTERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            13891..14835
                     /locus_tag="ACHA5T_003005"
     CDS             13891..14835
                     /locus_tag="ACHA5T_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888476.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            14839..15042
                     /locus_tag="ACHA5T_003006"
     CDS             14839..15042
                     /locus_tag="ACHA5T_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HFM8888477.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMLVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(15342..15578)
                     /locus_tag="ACHA5T_003007"
     CDS             complement(15342..15578)
                     /locus_tag="ACHA5T_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8888478.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(15761..16132)
                     /locus_tag="ACHA5T_003008"
     CDS             complement(15761..16132)
                     /locus_tag="ACHA5T_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HFM8888479.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(16129..17457)
                     /locus_tag="ACHA5T_003009"
     CDS             complement(16129..17457)
                     /locus_tag="ACHA5T_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM8888480.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(17418..17747)
                     /gene="mobC"
                     /locus_tag="ACHA5T_003010"
     CDS             complement(17418..17747)
                     /gene="mobC"
                     /locus_tag="ACHA5T_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFM8888481.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(18005..18376)
                     /locus_tag="ACHA5T_003011"
     CDS             complement(18005..18376)
                     /locus_tag="ACHA5T_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8888482.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            18521..18706
                     /locus_tag="ACHA5T_003012"
     CDS             18521..18706
                     /locus_tag="ACHA5T_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFM8888483.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            18864..19031
                     /locus_tag="ACHA5T_003013"
     CDS             18864..19031
                     /locus_tag="ACHA5T_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888484.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            19649..20308
                     /gene="vanR-B"
                     /locus_tag="ACHA5T_003014"
     CDS             19649..20308
                     /gene="vanR-B"
                     /locus_tag="ACHA5T_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-B"
                     /protein_id="HFM8888485.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            20311..21654
                     /gene="vanS-B"
                     /locus_tag="ACHA5T_003015"
     CDS             20311..21654
                     /gene="vanS-B"
                     /locus_tag="ACHA5T_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-B"
                     /protein_id="HFM8888486.1"
                     /translation="MERKGIFIKVFSYTIIALLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQAQSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
BASE COUNT         5260 a         5828 c         5963 g         4624 t
ORIGIN      
        1 gggcgcggga aaggaggtgc tacccgtgat tagtgaaatc attgaagaat ggataaaagg
       61 catcctcatg gacggtatca cgggcaacct gtccgggctg ttcgatacgg taaatgccaa
      121 agtcggagaa atcgccgccg acgtgggacg aaccccgcag gcatggaaca gcggtatttt
      181 caatatgctg cacagcctgt cggaaaccgt gatcgtcccc atagccgggg ccatactcgc
      241 ccttgtcatg tgctatgagc ttatccagat gataacggaa aagaacaata tgcacgactt
      301 tgacagctcc atgttcttcc gctggatatt caaatccgcg tttgcaatcc tgatcgtaac
      361 gaacacatgg aacatcgtca tgggcgtatt cgacgccacc caaagcgtcg tcaatcaaag
      421 cgccggtgtg attatcgggc agacatccat tgattttaac accctgatac cagaccttga
      481 aagccaattg gaggctatga gcgtgggcgc tcttttgggg ctgtggttcc aaacccttgt
      541 tgtgggcctc accatgaata tcctttccat ctgcattttc cttgtgacct atggccgcat
      601 ggtggaaatc tatgttgtca cgtcgctggg gcctatcccc cttgccacaa tgggcaacgc
      661 ggaatggcgc ggcatgggcc aagggtatct gaaatccctg ctggcgctgg gcttccaagc
      721 ctttttaatc atggttgtgg tgggtatcta cgcggtgctg atacagaata tcggttccgc
      781 ttccgacata tccggcgcaa tctggggctg tatgggctat acggtgctgc tgtgcttctg
      841 cctgttcaag accggcagta tatcaaaggc cgtattcggg gcgcattgat cccaaaatcc
      901 aagcatacac aggaggaact tgcttatttc aacgtacaac atcatttacg ctgacccgcc
      961 gtggcggtat gactgcaaaa acgtacaggg cgcagcggaa aatcactatt ccaccatgag
     1021 cattgacgag ctgtgcgccc tgccggtgga acggctggcg tccaaagact gcctgctgtt
     1081 tttgtgggcc acattcccca tgctgccgga agctctgcgg ttaatcaaag cgtggggctt
     1141 ttcattcaag accgtggctt ttgtatggct gaaacagaac cgtaaaagcc ttacatggtt
     1201 ttacgggctg ggccgctgga caaggggaaa cgcggaaatc tgcctgctgg caacacacgg
     1261 caagccgaag cgtcgttctg cctctgtcca tcagtttatt atcagcccga ttgaacaaca
     1321 cagcaaaaag ccggatgtga cccgtgaaaa aatcgtagag ctggcgggcg atttgccccg
     1381 cgtggagctg tttgccagac agaaaacccc cggctgggac gtgtggggga acgaggtgga
     1441 atgtgacctt atcctgacgt gaaaggaggt gccaaatggc ttatgtaacc gtccccaagg
     1501 acttaaccca tgtgaaaagc aaggttgtgt ttgggcttac caaacggcaa ctgatctgtt
     1561 tttccggggc gctggtgatc ggcgtcccct tatattttct cacccgtgac tatcttacca
     1621 acagcgcggc ggccctgctc atggtatttg ccatgctgcc gggctttttg cttgccatgt
     1681 acgagcgtca cggccagccc cttgaagtgg tgattatgca gatcatccag tgctgcttca
     1741 tccagccaaa ggagcgtccc tatcagaccg acaacaccta caccgccctt gtgcggcaat
     1801 accagatgga acaggaggta aaggccattg tccaaaaagg aaatcccaag agaaaccgaa
     1861 aaaacaaagc tgacccgcgc ccagcgaaaa gaaattgacg cggtaatccg taaatataag
     1921 ggcgacggca ggccccacac ggcgcagcag agcatcccct acgaggtcat gtacccggac
     1981 ggcgtgtgcc gggtatcgcc cggcgtcttt tccaagtgca ttgagtttgc ggacatcagc
     2041 tatcagcttg cccagccgga cacgcagacg gctatctttg aaaaactgtg cgacctgtat
     2101 aactatgtgg acgcctccat tcatatccag ttttctttcc tcaatcggaa agttgacccg
     2161 gtgcagtacg ccaagagctt tgagatcgcg ccgcaggggg acgacttcga cgacatccgg
     2221 gccgagtaca ccggcattct gcaaaagcag cttgcaaacg gcaacaacgg catggtaaag
     2281 accaaatacc tgacattcac catcgaggcc gaaagcgtga aagcggcgcg agccagattg
     2341 aagcgcatcg gctttgacct gttaggctat ttcaagagca tgggggccgt agcccatgtg
     2401 atggacggct gggaacggct gaacctgctg catggcgtgt accacccgga cggcgagata
     2461 ttcaattttg actggaagtg gctggccccg tccggcctct ccaccaagga ttttattgcc
     2521 ccgtcctccc tgtgcttcgg caatgcaaag acattcggca tgggcggcaa atacggcgcg
     2581 gtaagtttcc tgcaaatcct gtccccggag ctgtcggacg atatgctggc cgacttcctc
     2641 aatacggaaa gcggtgtttt agtcaatctc catgtgcagg ccattgaaca gaccaaggcg
     2701 atcaagacca tcaagcgcaa aattaccgac cttgacgcta tgaagattgc ggagcagaaa
     2761 aaggccgtcc gcagcggtta cgatatggac attcttccca gcgaccttgc cacctacggc
     2821 gaggacgcca agaagctgct gacaaagttg cagacccgca atgaacggct gttccagttg
     2881 acgttccttg tcctcaacgt ggccgacacc aagcaaaagc tgaataacga cgtgttccaa
     2941 gccgcaggcg tggcccaaaa gcataactgt ccccttgtcc ggctggacta ccagcaggaa
     3001 cagggccttg caagcagcct gccgctgggc gtcaatcaga tcaagataca gcgcagcctt
     3061 acgacttcca gcgtggccgt gttcgtcccc tttgtgacgc aggagctttt tcagggcggc
     3121 gcggccatgt attacggcat caacgccaag tcccgcaaca tgattatgct ggacaggaag
     3181 caggcccgtt gccccaatgc cttgaagctc ggcacccccg gcagcggcaa atccatgagc
     3241 tgtaaatccg agatcgtcag tgtgttctta acgacgcctg acgatatttt tatttcagac
     3301 ccggaggccg aatattaccc ccttgtcaaa cggctccacg gacaggtaat ccggctgtcg
     3361 cctaccagca aggactttgt gaacccgctg gacatcaacc tgaattattc ggaggacgat
     3421 aacccgctgg ccctgaaatc cgactttgtg ctgtcgttct gtgagcttgt catgggcggc
     3481 aagaacgggc tggaagccat tgaaaagacc gtgatagacc gggctgtgcg cgtgatctac
     3541 cggccctatc tggcagaccc ccgcccggag aatatgccga tcctgtccga cctccacaaa
     3601 gcgttgttag atcagcatgt cccggaggct gaccgggtgg cgcaggccct tgacctgtat
     3661 gtgtccggct ccctgaacgt atttaaccac cgtaccaacg tggacatcgg caaccggctt
     3721 gtgtcctttg acattaagga gctgggcaag cagctcaaaa agctggggat gctcattgtc
     3781 caagaccaga tatgggggcg cgtcacggcc aaccgaagcc aaggaaaggc cacatggtat
     3841 tttgccgacg agttccattt gcttttgaaa gaggaacaga cggcggccta ttctgcggag
     3901 atttggaagc ggtttaggaa atggggcggc attccaacag gtgccaccca gaacgtcaaa
     3961 gacctgcttt cctccccgga aattgaaaat atccttgaaa acagcgactt cattacgctg
     4021 ctcaatcagg cgtccgggga tagaaaaatc cttgcggaac gcttgaattt gtccaccgaa
     4081 cagcagaagt acatcgacaa ttccgaaccc ggcgagggcc ttttgatctt tgaaaacgtg
     4141 gtgctgccct ttaccaaccc catcccgcac aacacccagc tttacaagat tatgacgacc
     4201 cgcttaaacg aggtggcggg agtatgaccc ggcgacgcca caaaggaggt gaaccaccat
     4261 gaaagagctg aaagccaagg cgaaagtgac gcaggccatg acccgcgacg ggctgacgct
     4321 ggaaaatcag gccaccggcg aggtggagaa catttccagc cgggaggccg aacaggattt
     4381 gtccgcgccg gggccgggcg gcaccgccga taagctgctg gaacgggccg acgccatcca
     4441 tgacggccat aaggccaaga aagccgcgaa gcaggccggg gctgtggccg ccgaggggga
     4501 cgctgccctg caccggccat ccgcccggct ccaactgtcg gaggaagaac gggccgaccc
     4561 ggatttacag ccctatatcc gaaaatcgga caaacgggcc gataagctgg ataaggcccg
     4621 cgccgccatc cccaagaagc gcgtcccggt caaggaaacc gtctttgacg aggcaagcgg
     4681 caaagcaaag accgtcctgc actttgacaa acaggacaag gggcctccct ccctgaaacc
     4741 caatccggca ggccgtcctg tttccgaggt ttttctattc ggccacggga aaatccatga
     4801 agtcgagcat gaaaacgtgg gtgtggaggg cggccataag ggggaggaac ttgccgagcg
     4861 gcaggccgga aaagccatcc gcagcaccat ccggcaccac aggctgaaac cctactggga
     4921 cgcggaaaag gccgagcgca ggctattgtc tgccaacgcg gagtatttct accagaaatc
     4981 tttgcgggat aacccggaga tcgcacaggc ggccagcaac cccatttccc ggttctggca
     5041 gaaacagcag atcaagcgcc agtacgccaa ggccgcccgg acagcgggcc agaccgcagg
     5101 cgcggcggca cagggcgcgg ccaagacagc cggggcctcc acgaaagcgg ccaaggccgc
     5161 agccaaagaa agtgaacggg cggcctcctt tgtggcccgc cactggaaag gggcgctggc
     5221 cgtccttgtg ttggggcttt tgatgctgct tatcatcggc ggcttacagt cctgcaccgc
     5281 catgttcggc agcgccggaa gcggcatcac ggcgtcctct tacttttccg aggatagcga
     5341 tatgttggcc gccgaggacg cctacgcgga gctggaagcc gagctgcaag actatctgga
     5401 caactacgag gccacccatg actatgacga ataccatttt gaccttgacg agatcagcca
     5461 tgacccctat gtgctgattt ccattctgtc ggcgctccat gacgggatat ttaccatcgg
     5521 ggaggtacag ggtgatttgt ccatgctctt tgacaagcaa tatatcctga cagaaaacgt
     5581 cgtggtagaa acccgttacc gcaccgaaac cgatacatgg acggacgcgg agggcaacac
     5641 ccatacagac acctaccaag tgccgtatga ctattacatc tgctacgtca cgctggaaaa
     5701 cttcgacctg tcccatgtgc cagtctatat catggacgag gaaacgctaa gtttctattc
     5761 cctgtatatg tccacgctag gaaaccggcc tgatctgttt tccggcagcc agtaccccaa
     5821 cgcctccacc ctgacgccgc cgacctacta cgacgtaccc ccggaggctt tggaggatgc
     5881 cacctttgcc gccatgctca aagaggcgga aaaatatatc ggttatccct atgtgtgggg
     5941 cggcagctct cccagcacca gctttgattg cagcggcttc atttcgtggg taatcaatca
     6001 tagtggatgg aacgtgggac ggctgggggc gcagggccta tataacatct gcacccctgt
     6061 ttccagttcg caggccaagc ccggcgacct tgtatttttc aagggaacct acgacacccc
     6121 cggcgtgtcc cacgtcggcc tgtatgtggg caacaatgtg atgctgcatt gcgggaaccc
     6181 gatttcctac acgaacctta cttcaaacta ctggcaatcc catttttaca gttacgggcg
     6241 tttaccttaa cgccagaaag gagctttcaa ttatggcaac caaacttgac cgcatcgaaa
     6301 aggacatcca gaaaaccaag tctaagatcg cggagtttca gaaacagctt cgggagctgg
     6361 aaacgcagaa aaccgaacag gagaatttgc agatcatcca gcttgtgcgc ggcatgaaca
     6421 tgaagccgga ggaatttgcc gcgttcttac gcagcggcgc aatgcaggcc gcccctgccg
     6481 caacccctta ccatgaacag gaggacaatg cccatgaaga ataagaccag acggatttcc
     6541 cgtattctcc ccgcacttct ggccgtgatg ctctgcgtca cggcctttcc cgttaccgcc
     6601 cttgcaggcg gcaatgaccc ggcaccggcc ccgctgccgg aggctacaac cgaacccgct
     6661 acgggcggtt tggagcctga aacagacgaa accggcaccg ccacggacgg gacagaaacc
     6721 gaggccgaaa aagataccgg ccctctgacc ggcaaggaca tcaccgacct gttttccgcg
     6781 ctgttcggtt ccaaggtgag cattgccgcc acggacgacg gcatccagat caccaccggc
     6841 accgacaaaa aagaacctga acagaccggc accgtcacca ccaacggcgg caggctgaat
     6901 gtccgcaccg gcgcggggct tgacaagacc gcgttcaccc agcttcccaa tgggacgacg
     6961 gttgaggtag tcggcacaga cggcgactgg attaaaattc tcttgccgga gcgcatcggc
     7021 tatgtccatt ccgactatat gaccgtcagc gaaaaggaag tagcggctac cggcgagggc
     7081 ggtttttccc tctccattga cccggaggaa attgcttctc tgctggaact gttcaatggc
     7141 ggcggggcca gcgccgccct gtccccggac gggaacctgt cactgattga cgacatcggc
     7201 agcgccgcga aaaatggcaa gcagtttatc accgtggaaa ccaaaaacgg caatgtgttt
     7261 tatctgatta ttgaccggga cgacgaggga aaagaaactg tccatttctt aaatcaggtg
     7321 gatgaagccg atctgctgac gctgatggag gacggggaaa ccgtcacccc cgctgctgtc
     7381 tgctcctgta ccacgaaatg caaggccggg gctgtgaaca cgaattgccc ggtatgtaaa
     7441 accaacctga ccgagtgcag cggcccggag ccgcaggaac cccagccgga ggaacccgaa
     7501 gccccggagg aagaacccaa gggcggcgcg ggcggcctga tcgtgttcct gctggtggcg
     7561 ctggcgggcg gcggtgcggc cctttactat ttcaagttca aaaagcccaa ggctgaaacc
     7621 accggcagcg acgatttggg cgagtatgat tttggggagg acgaggattt ggaggacgag
     7681 gaacccgaaa ccgagtttga cccggaagct gattttgtga gtgaacagga ggacgaggaa
     7741 tgacatataa acttgtaatc gcggaaaaac ccagcgtggc ccgcagcatt gcgggcgtga
     7801 tcggggccac ggagaaacac gacggctatt tggaggggaa cggctatctg gtgagctggt
     7861 gcatcggaca tctggtgtcc tttgccgacg ccggacgcta tgacgagcgt ttcaaaaaat
     7921 ggcgctatga ggatttgccc attctcccgg aaacatggca gtacatcgtc cccgacgaca
     7981 agaaacagca gtttgacgtt ctctgttcgc tgatgcagcg ccccgacgtg accggccttg
     8041 tgtgcgccac cgacgccggg cgcgaggggg aattgatttt ccgtttcgtc taccagatgg
     8101 cgggatgcca aaagcccttt gagcgccttt ggatttcctc tatggaggac agtgctatta
     8161 aggacggctt tgcccacctg aaaccgggcg gcgactatga tccgctgtat caatcggccc
     8221 tgtgccgggc caaggccgac tggctgatcg gcattaacgc cacccggctt ttttccgtcc
     8281 tgtaccacaa gaacctgact gtgggccgcg tccagacgcc gaccttaaaa atgctggtgg
     8341 acagggacgc caagattacc gatttcaaga aagagaaata tcacatcgtc catatcacgg
     8401 cgggcggcgc ggacgcggta agcagccgct tttccgacgc tgccgaggca aacaccgtca
     8461 aggcggcttg tgcaaaagcg caggccgttt gcgtttccgt gacgcgggag aaaaagacgg
     8521 aacagccgcc caagctctat gaccttacca ccttgcagcg ggaggctaac cggcttttcg
     8581 gctataccgc caagcagacc cttgactatg cccaggcact gtatctaaag aagctgctga
     8641 cctatccgag aacggatagt caatatctta ccgacgatat gctgcccgcc gtggaaagcc
     8701 ttgtgtccgg cctgtgggag ctggtgccgt ttgccaaggg cctgaacctt tccccgcagt
     8761 ttgaccgcat cctgaacagc aaaaaggtgt ccgaccacca cgccattatc cccaccgctg
     8821 aatttgtgaa gcagggcttt ggcactctgg ccgagagtga aaaaaagctg ctgtccctga
     8881 tctgctgcaa gctcttatgt gccgtggccg ggccgcatat ctatgaggcc gtcaccgcca
     8941 catttacctg tgccgggcag gagtttaccg ccaagggcaa gaccgtcctt tcccccggct
     9001 ggaaagagat tgaacgccgg ttccgttctt ccttaaagac cgacgccgac gaggacgccg
     9061 aggcggtgcg ggagctgccg gagctttgcg agggccagac ctttgcagac gtggccgcct
     9121 ctgtcaccga gcatttcacc acgccgccca agccctacac cgaagatacg cttttgtccg
     9181 ctatggaacg ggccggggcc gaggatatgc cggaggatgc cgagcgcaag ggattaggca
     9241 caccggccac ccgcgccgcc attctggaaa agctggtgca gatgggcttt gtccagagaa
     9301 aaggcaagca gctcattccc accaaggacg gcatcaatct ggctgtggtg ctgccggagg
     9361 cgcttacttc tccccagctt actgccgaat gggaaaaccg gctgaccgag atcgccaagg
     9421 ggcaggccga tcctgacgaa tttatggacg gcatcgagac gcaggcgcgg gagctggtgc
     9481 agacctattc ctgtatcagt gaggaaaagc agaaattgtt tcaggccgag cgtgttccta
     9541 tcgggacgtg tccccgctgt ggtgaggctg tctatgaggg caagaaaaac tactactgcg
     9601 gaaaccgggc ctgccagttc gtcatgtgga agaatgaccg attttttgag gaacgcaaga
     9661 aagcgttcac cccgaagatc gccgctgcgc tgctcaaaac cggcaaagtc aaggtaaagg
     9721 gcctctattc cgtaaagacc ggcaaaacct acgacgggac ggtgcttttg gctgataccg
     9781 ggggcaagta cgtcaactac cgcattgaac acaggagcta aaggacagta acacagcaag
     9841 catagggagt gtggctacac tcccggtaaa atcccccggc cgcgtccctg ccgggccggg
     9901 gatttttgct tgttggggaa gtccccaacc ccctattttt tgaaacagaa cagagaaagg
     9961 agtgaccgcg tgaacgaagt attttctatc caagtccata accggcagtt tttggaacag
    10021 ggcaaacaag gcgtctggct ggatttgccc accacaaccg aaacgctgca agccgccctg
    10081 cgggatgttg gcattactgc cgacaatccg caggattttt ttatcaacgg gtattcctgc
    10141 ccggaggaca gacaccttgc cattccctat gacatggtgc tggccgccga cgtggacgag
    10201 ctgaatttcc ttgccgcccg gctgggccag cttgacgccg ccgagatcgc agagctgaac
    10261 gccgccctgc aaaatccccg tggcggcttt gagaatatcg gccagatcat cgactacacc
    10321 gaaaatgtgg attactatgt gcatcttccc gacgttaggg gggatggaca gttgggcgac
    10381 tactacctga accgttccgg catggtggat atgccggagg aatggaaagc cgggattgat
    10441 acggcgcagt ttggacgcca cattgcccag caggaacaag gcgtttttac ccagtacggc
    10501 tatctggtga gaagcggcga cgaatggcag cgcgtccatg agggccagcc ggtgccggag
    10561 gaataccggg tgatgcacta cccccagccg gaagctgacc gggaggaacc gcaggcccag
    10621 cagccggagg catccgaaag ccccgacaaa gcgccgcccc cggttatccc gatcatgctg
    10681 gacggcatga acagcaaaga acgcatgaaa gtgattactg accgtctgga aaccggcgta
    10741 caggagcttt ttgaaagtag ccgctatatg gcttacttat ccgctatgtc caagatgcac
    10801 gattacagtc tgaacaatac gctgctgatc gccatgcagg gcggttcgat tgtggcgggc
    10861 tataacaggt ggcaggatga atttgaccgc catgtgaagc ggggcgagaa aggcattaag
    10921 atactggccc cggccccgtt caaggttaag cagcagatgg agaaaatcga cccggccacc
    10981 cagcagcccg tgatcggcgc ggacggcaag cccgtgaccg aagaacagga agtgacggtt
    11041 ccggccttta aggtggtgac agtctttgac atcagccaga ccgagggcaa ggattttccc
    11101 atgccctata tcagtgaact taccggcgac gtgcagcagt atcaggattt ctttgccgcg
    11161 ctggaacgga catcacccta tcccataagc attgagccga tttcagacgg ggccaagggc
    11221 cgctgcttct acgcaaaaca gcgcattgcc atcaacgagg gcatgagcga attgcagacc
    11281 ctaaagaccg ccatccatga gattgcccat gccaagctcc acgccattga ccgggaggcc
    11341 cccgccgagg aacaggcggc ccggccagac cgcagcaccc gcgaggtaca ggccgaaagc
    11401 gtggcctata ccgtctgcca gtattacggg ctggacacgt cggactattc ttttggctat
    11461 gtggccgatt ggagcagcgg cagggagtta tccgagctga aagcctccct tgaaaccatc
    11521 cgggccacgg ccagcgaatt gataaccgag atcgacggcc attttgccca gcttcagcag
    11581 gagcgcgagg cccggcagga acaggaggcc gtcacggttg acgttacccc ggaggcagcg
    11641 gccccggcgc aggccgagga agaacaggcc ccggcccttg atccggcagc cgagccgctt
    11701 gtgactgtgc tatggagcga aagcccccag ctaaaggacg gccagcaaat gccgttgcat
    11761 gaggccgacg cgctgtttgc ccgtctggat gcagcatatc cggcagaggc aggctatgac
    11821 aaaaccgcgt tccgcattga ctgtatcttt cagggcgagc cggacagcta cgaggggcgg
    11881 caggattttg gagataggga cggttccctg atcgaacata tccagagcta ccaccaattt
    11941 tacgcgcagg acgaaaactg gaaaaagcat gtaatcgcta caaccggcat agagggctgg
    12001 gagcaggaca aggcccatcg ggatatgctg ttaggcgagt ttgtccccta tatgaagctg
    12061 cattgcagcc ttgcccaaat ggaacagacg gcgcagggcc gcttacagtc cggggaaacc
    12121 ctgacgccgg aggaaacggc ctatttgggg gcgcttctct cttatgtgca ggagtgccgc
    12181 cccatgctca atcagggcca gtatcagttg ccggagccgc cgaaactggc tgacttcgac
    12241 cagagcttgc aggactacaa agcacaggta caggccgaga tcgcgcagga ggccgccgca
    12301 gccggtatga ccgtggagga atacgcggcg gctggctatg aagcacccca acaggacacc
    12361 ttttccatct accagcttcg aggcggggag gaaacccgcg actatcggtt tgagccatac
    12421 gagcgtttac aggccgcagg gctggccgtg gacagtgcca actatgacct tgtttatacg
    12481 gcaccgctgg ataaaagcac gtctttggag gatatttacc gcacattcaa catcgaccac
    12541 ccggcagatt ttaggggaca cagcctttcc gtttccgacg tggtggtgct gcaccagaat
    12601 ggacaggaca ccgcccacta tgtagacagc ttcggctata tggaagtgcc ggagtttttg
    12661 cgggaaaatc ccctgcgtac cgccgaactg tctacggagc agaacgaaaa catgattgac
    12721 ggcattatca acaatacgcc gcccgaaccg cagccggagg aacccgaaca gctatctttt
    12781 gaggatgtgg cagttgcttc cggccagaca caggaggaac ccccggaggc tgcgcccccg
    12841 caaacggctg tccgctatta tcccatcaat gagggagccg cccgccgcgc caaggaagcc
    12901 atgagctttt ctgactaccg gcccggcagc gccacggcgg aataccggca ctatgtcgat
    12961 caggccgtgg aaattgcgga gcgccagaaa aagcgtgtgg agccagagta tcacgaaaag
    13021 atagacagcc tccttgacac ctacgcccgc aagctggccg agaacatgaa taagggctat
    13081 gagattaccg cccgcgtccc ctctgtcctg attgccgggc cgtccaattt cccggtacgg
    13141 gcgaaagaaa agcagaacgc cgccagtgac cgcaacatgg aggaatttca gtacattcag
    13201 ggcttgcttg acaaaatccg cagcaccggc atgggtggta tcagcgccga cgatccgcag
    13261 gccgtttcca agctggaaaa gaaactggaa aagctggaag cgtcgcagga gttgatgaag
    13321 gccgtcaatg cctactaccg caagcatggg acgctggacg gatgccccca tctgaccgaa
    13381 agaggcattg aaaacctgaa agcggatatg gcgtcgggct ggcactatga gaaaaaaccg
    13441 ttccagtcct ggcagctctc caacaataac gccgagatac gccggttaaa gggccgcatt
    13501 gaggaattga cccgccaaaa ggaggcggcc tatgtgggat gggagtttga cggcggcact
    13561 gtggagatca accgggtggc caaccgcctg caaatcttct ttgaggggaa gccggatgct
    13621 gctgtacgcg acgagctgaa aagcaacggt ttccgctggt cgcccaaggc cggggcatgg
    13681 cagcggcagt taaacgacac cacaatccgc gtcgctgacc gtatcaagtg catccagccc
    13741 ctttccggcg aaaagcccag cgccttacag cttgcggccc gcagggagaa agaaaagccc
    13801 tctatccggg cgcagctcaa cgcggccaag accgaacagg ccaagcagcc accggcgcgg
    13861 gaaaaatcaa agggattgga ggtagagtaa atgcagaagt ttgaaaatgt ggacgttctg
    13921 gccgcgctgg aacagatcat gcggcagaac accgcgtttt atcaaagtga ctttggcatt
    13981 gacaaagaca ttatcaggcg ggatgccgcc agcgatcagg ccgcagacaa gaccctgctc
    14041 tggatgtccc ggccctccgg gacgtactgt ttccgggaac gtgatgtttt cctgaaagac
    14101 acccgacagt acaacacatg gaaattttac ggggagcaga cccgtgacaa gatactggcc
    14161 tacgcggtgg agctgaccgg cacacagggc ggcactatcc ggggcaatct ctatgagctg
    14221 gactatccgc agcatttccg gcacgtcatt gaaaccgccc agccggtgag tgtgaaccgg
    14281 ctcttttatg agcatggcac ccgtgacatc ccggagagcc agcgttttga cggaagccct
    14341 gaccgggtat tgggaaactt tttgtactat gaggcccagc cccatgaccc ggctgtgctg
    14401 caggaggcgc tacggacgga gcagcacagc cgggggcggg ccgtccccgg cgatttcaaa
    14461 gtccacgtca ccgctttgca tgacagccgg attgaaaccg aggcccggcg tattgtggac
    14521 agcctgaaag atctgtccgc gcctaacagc cccaataaaa cgcatttcat ggtggagatc
    14581 tccccgcact ttgcggcgat tgcttccacc aaggataatg accggctctt tgccatgctg
    14641 ccctataaat ccctgtgttt taccgggatg aaaggcaggc atggcgtgta tgccgtgatt
    14701 aaccgggacg agcgccggga tgtgagtatt cgcaggcccc ggccctctat ccgcaagcag
    14761 ctatccgaca gcaaacaggc cgccagccca aaaaaggcgg cggccagaac caaaaagaat
    14821 gaattggagg tataaggaat gatgcacttt accgtggagg aagaaaacct gatctgtatg
    14881 taccacaatg cagaccggcg cagaacgata aacaggctca ccgccgtcat gctggttatg
    14941 gatggggata tgcgggcgca ggcccagcag accctttcca agctggaacg catgacggat
    15001 gccgactatg acggccagaa gttccacttc acagacgaat aacgaacagc agacgccggg
    15061 cgcggcggcc atgtggtcgc ttgcgtccgg cgtcttttct ttttgcctat tcctacgcat
    15121 ttagaacccg gtattgtcgg ggataggatg aagtattcgc ttgcccctgc aaacatgcgg
    15181 gagaagcctt gcagcgggcg gttttcaatc ccttaaatgc gtaggttgca gctataatta
    15241 gacccctgaa acagatatag ccgcgcagcg ggtgtatctg tttcaggggt gtgcaggtga
    15301 tagccgcaaa gcggcgtaag agggtgcagc cctcttgccg tctatctctg ttccagcggg
    15361 aagctcggca atgcttccag cagtttcagg gaaatgcgct gccgtatatc ttcgtcaatg
    15421 actttcttct ttttgccgtt tgcctgtctg atctccaccg tggaaagctc gttgatgtag
    15481 tcagcataat gggccaacac tttttccgtg gcccacctct caccggcaac ggcggctttg
    15541 atcgtttcat agtccagcag cttttcttgt ccgtccatcc tgataccccc gtcagactga
    15601 ttttttctat gataccatat tcggaagcat ggaaccagag aaaacggcgc ttttctgctg
    15661 gtaaattcta cgcatttaga acagcacttc ggcggggata ggataaaaca cccacatacc
    15721 ccgctgacca tgccggaaaa gccttgaaat ataagggctt ttacagctct aagtgcgtag
    15781 gtttccagta tcttttccgc tggcgctcgg cctctttgcg gcggcgcatc cgtttggagc
    15841 aggccgcgca gtatttggcc cggttagact tcggcgtgaa cgtggccccg cactcacagc
    15901 agcgtttcac ttcgtcccgg tctttggtga tctcggcata cagggccgca tccagcggga
    15961 ggacagcggc cttaaaccag cggcacagaa gcgaatagga aatgctctgt acgcaaatac
    16021 attcttcgcc gtcgtccagc aacaggcagt tgccgtcaca atagttgcag cactcatgcg
    16081 tcagcttccg ggccttgcga tattgagggt aattcattcg tggaatactc atagctcttg
    16141 cgtccattcc tttcccggcg tcatacgcaa aatgccgtcc acattctgct tgatgatgcc
    16201 gtattcctgc atctgctttt tcgcttcgcc gtacttctca tacagccgtt ccttttcctc
    16261 ggccaatcgc ttgtactcgg ctttcagggc gtacagatcg gggagctttt tcacaccctg
    16321 tccctttagg gcctttgccg cagcttcata caaaatcaac gtgctttcat gctcacgccg
    16381 gaattttgcc ttatccgctg cattgcggta ttccatagcc acgggccaga gctgcttgta
    16441 ggtagaaata ttctttatca gcaccgccat atccgcaagc cggtgttcca tagctttgag
    16501 ggtagcggcg gcctcgtcgc tggcggcggt gatctcggcc actttgcttt ccaaatccgg
    16561 gtaggtgtct atcccatgtt cagtcaggaa attcaaagtc tttgcggcct gcttcaaatt
    16621 gtggagcttc gcccacctct catagcctgc cgactgctgg gccttaatgt tgttttccag
    16681 atcaatccgc aaagaaatcc ccttgcgctc tttgggcgct ctggtgataa cgcggccttt
    16741 gatacgctct ttgatggctt cctcggtgta ggcttccccc aatgtcttgc agcgggtaaa
    16801 gcgttcctgt ccgggagcgc ggaaagaaat aaactttccc ggcttgatct catagccttg
    16861 cgcctgcatg agccgcagga aatcatcaaa atcttttgcc tgcgggatag cggcgtcaat
    16921 ggctgctttg agcttcgctt tccagctcgt ccctttccgc tgggcgtccc actcggcata
    16981 gctcttgccc ttactctggc cgggcttcac cacggacaat ccgttttcct tgcacagcct
    17041 gtcgctggtg cgccggatat aggcatagct ctgtttgttg gaaatgtact tgcgctgatt
    17101 taccatatcc acggcacaga aaataatgtg attgtggacg tggcccttat caatatgggt
    17161 agtcagcaca tagggatatt tcccttgcag cacttcctcg gcaagctgct tcccgatctc
    17221 atgggcctgt tccggcgtgg cttcgccggg ggcaaaggat tgtatcaggt ggtggcccag
    17281 attattgccc ttttggtatg cctgcgccag cagcatttca aattcaatgt ccgctgtttc
    17341 ataagagcag gcaaaggagg ccaccagcag cttgccgtca gttttgtccg ggttctcgat
    17401 gtagtcaagg gcttttttca gcgttgactt gataggatgt atctttgtaa ctgccatatc
    17461 tgcgccagcg ccccctttat atcctcgatg tcctgtgcgt acaccggccc cataccgttc
    17521 agcctctttg caatctgatt gacgttgacg ccgatcttct gtatctcggc ggtctgcgcc
    17581 cggatgtcgc tggtgtccat gttgacgata tagccgtcaa tgagcatctt ccgggcgtag
    17641 gctgaaaagt tcttcgtatg cagcagcgcc attttctgtt caatcatagc ccgttcctcc
    17701 ggcgtcacag ggacgcgcag cacgattttt cgctttctgt ttgccatgct tcaccgtcct
    17761 ttccattcag gggtttgggg atttccccaa caagcaaaac ggacgcggcc cggcagggac
    17821 ggggcaggcc gtttttccgg gagtggctac actccctatg cttgctacgt tactgtttct
    17881 ccactaacaa gacgtttcca aagccccaaa ctacccgcaa aatagccctc tggaaagaaa
    17941 aaagtgcaaa aaaatagaag ctacgctgtg cagctcccgc taaaagtaaa acagaggatg
    18001 ccagttacac atcctccgtt tcattcttct tcggcttttc aattagtatg gcgctgtcaa
    18061 tcgcgccctc gatgatcggc aggtattctt cggggcagag tttcagcttg tggctgaccc
    18121 tttgccgcag ttcgctttcc tcccgcatga tctccgggtt aaagtagcgt tccacaggaa
    18181 gcccgcaggc tttaataagc tgtatgacaa ccggcagact gggaatggcc ccgtcgtttt
    18241 cgatgttggc aagatagcgc cagtcaatat ttattttctc tgccattgct ttgcgcgaaa
    18301 cgcgctttgc ttgccgggcc gctttcacat ccgcgccgaa agtttcaaag ccgggacaat
    18361 cttcaatatt cgccatatcg cacctcccac ttacattgta tatttcatac tctgcccgtg
    18421 gaatgttggt atatgcacct ttcttaaagt ttatatttca cattttgttt cttttattct
    18481 tcgagcaaaa gaattataat gtaccctgtg gaggtgtact atggactaca tgacgttgaa
    18541 agaggcaggc gagaaatggg gtgtgccccc acgatggata aactactatt gtgctgcggg
    18601 acgcatcccc ggcgctgtaa agatggcgac gatctggctc attcccaaaa ctgcggaaaa
    18661 gccgattgac aggcgtacaa agaaaggaaa ggcggttact gaatagtata atgaaaccgg
    18721 cgcattttgg atatggtttt gaagagagag aaagcatggt attttgtcac ggaaaaacta
    18781 taagatgata gatggcaggc tgctccagac cgataaaaag tattctcatc tgaaacagaa
    18841 agaaaagatt tacacatgga tgtatgaaga agcgaaactt tatcatgaca gaaatggaaa
    18901 atgcccgaaa caaagacgag gaagctgctg ctgtatcagc cgtatatggt agaattgaaa
    18961 aggccggcat ctggattctt tatggtgaag tctccagaca ttggataact gctggtctaa
    19021 tgcgcctttg agtatggggt gattgatttt aataccgggg aggtacggct ttttgaggaa
    19081 aatattctgg gatttacatt tggcgattat tacatttcct gtaactgcaa ggacacggat
    19141 gatgaagata tgaggaatat agacttgctg attttctact gtcttgaacg gtattaggac
    19201 tgaatctgct gcgcggagtg cggcaagagc gaccggaggg agtacacaca ggccctgcct
    19261 gtgtgaaaag ccataccgca aggtgcattc gcaaaaaaga ggccgcgcat tttgctgagg
    19321 cttggggtgt ccccaacaag caaaacgaga acgccgcagg cgggcaagtg gtggatcaga
    19381 atatccgtaa aggatattct gatccattgc ttgccagtat tatcgggatg ttttgacaac
    19441 cgctgtaatc gaaacatccc aaacattaag aaggtgttaa ttattattat cttgatttaa
    19501 ttcgtttaaa ccaataatat agatacatac accatgaaaa gaagcgagta tcccaatgac
    19561 ggatgattaa aatccgaaaa gaaatcgctt tgaaactaca gggaaactac agactgttat
    19621 gttatcttct taaaatggag ggatttttat gtcgatacga attctacttg tcgaggatga
    19681 tgatcatatc tgtaatacag taagggcgtt tctggctgag gcaggatatc aggtggatgc
    19741 ctgcacagat ggaaatgagg catacaccaa gttttacgaa aacacttatc aactggttat
    19801 tcttgatatt atgctgcccg gtatgaacgg gcatgaactt ttgcgtgaat ttcgtgcgaa
    19861 aaatgatact cccattctga tgatgacagc cctgtcggat gacgaaaacc aaatccgggc
    19921 gtttgatgca gaggcagacg actatgtaac aaagccattc aagatgcaga ttttactaaa
    19981 gcgggtggaa gccctgctgc ggcgcagcgg tgcgctggca aaggaaatcc gtgtcggcag
    20041 gctgacactt ctgccggagg atttcacggt actttgtgac ggtacagagc tgcccctgac
    20101 acgaaaagaa tttgaaatcc ttttgctgct ggtgcagaac aaaggcagaa ccttaacgca
    20161 tgaaatcatt ttgtcacgta tatggggata tgactttgag ggtgatggca gcacagtcca
    20221 cactcatatc aaaaatttgc gggccaagct gccggaaaat atcatcaaaa ccattcgcgg
    20281 tgtaggatac cgattggagg aatcataata atggaaagaa aagggatttt cattaaggtt
    20341 ttttcctata cgatcattgc cctgctactg cttgtcggtg taacagcaac actgtttgca
    20401 cagcaatttg tgtcttattt cagagtgatg gaattacagc aaacagtaaa atcctatcag
    20461 ccattggtgg aactgattca gaatagcgat aggcttgata ttcaagaggt ggcagggctg
    20521 tttcactaca ataaccaatc ctttgagttt tatattgaag ataaagaggg aagcgtactc
    20581 tatgccacac caaatgccaa tacatcaaat agttttagac ccgactttct ttatgtggta
    20641 catagagatg ataatatttc gattgttgct caaagcaagg caggtgtggg attgctttat
    20701 caagggctga caattcgggg aattgttatg attgcgataa tggttgtatt cagcctttta
    20761 tgcgcgtata tctttgcgcg gcaaatgaca acgccgatca aagccttagc ggacagtgcg
    20821 aataaaatgg caaacctgaa agatgtaccg ccgccgctgg agcgaaagga tgagcttggc
    20881 gcactggctc atgatatgca ttccatgtat gtcaggctga aagaaaccat cgcaaggctg
    20941 gaggatgaaa tcgcaaggga acatgagttg gaggaaacac agcgatattt ctttgcggca
    21001 gcttctcatg agctaaaaac gcccatcgcg gctacaagcg ttctgttgga gggaatgctt
    21061 gaaaatatcg gtgactacaa agatcattct aagtatctgc gcgaatgcat caaaatgatg
    21121 gataggcagg gcaaaatcat ttccgaaata ctggagcttg tcagcctgaa tgatgggaga
    21181 atcgtaccca tagctgaacc gttggacata gggcgcacgg ttgccgagtt gctgcccgat
    21241 tttcaaacct tggcagaggc aaacaaccag cggttcgtca cagatattcc agccgggcaa
    21301 attgtcctgt ccgatccgag gctgctccaa aaggcactat ccaatgtcat attgaatgca
    21361 gttcagaaca cgccgcaggg aggcgaggta cggatatgga gtgagcctgg tgctgaaaaa
    21421 tgccgccttt ttgttttgaa catgggcgtt cacattgatg atactgcgct tccaaggctg
    21481 ttcaccccat tctatcgcat tgatcaggcg caaagcagaa aaagtgggcg aagcggttta
    21541 ggacttgcca tcgtacaaaa aacgctggat gccatgagcc tccagtatgc gctggaaaac
    21601 acctcggatg gcgttttgtt ctggctggat ttaccgctca catcaacatt gtaaatattt
    21661 aaaacttaaa tgatt
//
LOCUS       DAYYGO010000318        16352 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.guided.064, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000318 DAYYGO010000000
VERSION     DAYYGO010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.guided.064"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..806
                     /locus_tag="ACHA5T_003016"
     CDS             <1..806
                     /locus_tag="ACHA5T_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM8888487.1"
                     /translation="SYKIIAENTGISEYQLKRIFTFIAGMSLMDYIKNRRLALANRDL
                     LDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISRYGIQKSFPRLTFFIDVRGGK
                     SMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQTITETQREEMHKLGDLYPNQV
                     INVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEMLAIEEHTWAIFPNKGPFPQTL
                     QDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVYSEIWIAVKAKK"
     gene            906..1388
                     /locus_tag="ACHA5T_003017"
     CDS             906..1388
                     /locus_tag="ACHA5T_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM8888488.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(1651..2118)
                     /locus_tag="ACHA5T_003018"
     CDS             complement(1651..2118)
                     /locus_tag="ACHA5T_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFM8888489.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            2241..3092
                     /locus_tag="ACHA5T_003019"
     CDS             2241..3092
                     /locus_tag="ACHA5T_003019"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8888490.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            complement(3343..5301)
                     /gene="nagE"
                     /locus_tag="ACHA5T_003020"
     CDS             complement(3343..5301)
                     /gene="nagE"
                     /locus_tag="ACHA5T_003020"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFM8888491.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(5298..6170)
                     /locus_tag="ACHA5T_003021"
     CDS             complement(5298..6170)
                     /locus_tag="ACHA5T_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8888492.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(6512..7384)
                     /locus_tag="ACHA5T_003022"
     CDS             complement(6512..7384)
                     /locus_tag="ACHA5T_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8888493.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            7512..8081
                     /locus_tag="ACHA5T_003023"
     CDS             7512..8081
                     /locus_tag="ACHA5T_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFM8888494.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLLVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            8148..8885
                     /locus_tag="ACHA5T_003024"
     CDS             8148..8885
                     /locus_tag="ACHA5T_003024"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8888495.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            9116..10384
                     /locus_tag="ACHA5T_003025"
     CDS             9116..10384
                     /locus_tag="ACHA5T_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFM8888496.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            10871..11335
                     /locus_tag="ACHA5T_003026"
     CDS             10871..11335
                     /locus_tag="ACHA5T_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8888497.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            11319..11948
                     /locus_tag="ACHA5T_003027"
     CDS             11319..11948
                     /locus_tag="ACHA5T_003027"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM8888498.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGVEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            12302..13780
                     /gene="msr(C)"
                     /locus_tag="ACHA5T_003028"
     CDS             12302..13780
                     /gene="msr(C)"
                     /locus_tag="ACHA5T_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFM8888499.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            13827..14273
                     /locus_tag="ACHA5T_003029"
     CDS             13827..14273
                     /locus_tag="ACHA5T_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFM8888500.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(14472..14987)
                     /locus_tag="ACHA5T_003030"
     CDS             complement(14472..14987)
                     /locus_tag="ACHA5T_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8888501.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            15104..16306
                     /locus_tag="ACHA5T_003031"
     CDS             15104..16306
                     /locus_tag="ACHA5T_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8888502.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         5150 a         2921 c         3408 g         4873 t
ORIGIN      
        1 tttcctataa aataatagcc gaaaatacag gaatctctga atatcaatta aaacgcatct
       61 ttacgtttat tgcagggatg tcgctaatgg actatatcaa aaaccgtaga ttggctttgg
      121 ctaatcgcga tcttctagat ggatcaagag tgacagaaac ggcgtttaga tatggatatg
      181 aaacagtcga aggtttttca agagcttttc gtgattggtc aggatatctg ccttcagaaa
      241 tcagcagata cggcatacaa aaatcttttc cacgcttgac attttttatt gatgtcagag
      301 gaggaaaatc gatggaattt cgtatcgaaa agaaagaaca gttttatgtg gcaggtgtgg
      361 cgaaaaaagt tcctgtcaaa tttgaaggag tcaatcaaga aatacaaaag ttagcgcaga
      421 cgatcacgga gactcaaagg gaagagatgc ataagttagg agatttatat ccaaatcaag
      481 taatcaacgt ttcttatgga tttgatggag accgcttgga agaaaagggc agtttgatgc
      541 acatgattgg ctttgctacg acaaaagaaa atccgtacgc tgatttagaa atgcttgcta
      601 tagaagaaca cacttgggcc atttttccta ataaagggcc atttccccaa actttacaag
      661 atacttgggg gaaaatctat gcagaatggc ttccttcttc agattatggg ttagtcgaag
      721 ctcccgagat ctcttttaca aaatggggag aggatttcag taatgtttac agtgagattt
      781 ggattgcagt gaaagcaaaa aaatagttaa aaaataaaaa tatccctaga aaaaagagcg
      841 taatcgtgtc atttcccttt aattttatgt aagatgaact taatataata gaaagggtgg
      901 gagaaatggc aaagtatagg atcgaagaaa aaccagattt tacggtgacg ggtattggtg
      961 tggaattgaa gagtagcaat acggattttg tcggtttggc tgcagagaaa caagcatttg
     1021 agcaaaaagt tatcggtaat ggaacaatag atgcattgaa acaagctgct gaaaatcatt
     1081 atttctttac agtcaacgaa ccctataacg ggaaaatgat gtactatgtc ggtgttcaaa
     1141 cgagtcagga tgttccaaac actacgcgaa tcatcccatt cccaaaagga acgtatcttg
     1201 ttgtaagcgc tttagcagaa tcgcctgaag aattaagaaa tgatttgacg aatacggcct
     1261 ttgggcatgt tttaagagaa gtagaagatt acgcctatgt tgggggtcca aatgctgtag
     1321 tcgaaatggg agttaccaac gggaaatata tgggagaaat ctggataccg gtagtcaata
     1381 aaccatgata atatagaggt cgtgaaagta gcgctttgac ctgagcatta gagaggaaaa
     1441 atgggaaata gctttccata ttttgcattt ttcagaacta atgccgaggg tcagctactt
     1501 gaacaccgtt tatcaagacg tcatgacttc agcgctttga cctgagcatt agagaggaaa
     1561 aaggggcatg acaaggcttt tgtcccccgg agaataaaag aatttatgta gacgattccg
     1621 atcagaaaaa tgcaggcaca gtagagagaa ttataaaaat ctctctacga ggcctgtgtt
     1681 ttctggtctt ttttcttttt ccaaaacggc tattttatgt atcagactat caagtgtgat
     1741 cttagccatt tcttcttctg cctttttctg cagccgagcg tagctttctt ctaaagcttc
     1801 ttggatattc gcaccaacga cgcaatttgg atttgttttt ggatcgactt ggataagctg
     1861 tgtattacct tcgatgctgc gatagacatc tagtaaagaa atctgatctg gacttttggc
     1921 aagggaaggc tgcggttttc cggtttttgt aaagatcaaa ccggattttt tgagctgact
     1981 catgatttta cgtacatttg ctgcatttgt ttcgatacta ctggcaatca tctcactaga
     2041 aagtgatcgg gtatcttgaa acatttcaat ataggcaagt acatggattg catcactaaa
     2101 ttgcagagaa tacttcatca gttgtcatcc tttcattttt tttgcttgac ttatcttacc
     2161 acactcttta tgataaaggt gtaatttata aatgtacagc tgtaaatctt aacaaataaa
     2221 aagaataggg agtggcaaat atgcattatt tagtaacagg agcaactgga ggttttggtg
     2281 gttatgcgct ggaattctta aaaaagatgg ttccacaaaa tgaaatctat gtactagtac
     2341 gtagtgaaga aaaaggagaa gcactgaaag aagctggatt taatatacgt attggtgatt
     2401 atgatgattt agactcaatg aaagaagcgt tgaaaggaat agatcgattg ctttttgtct
     2461 ctggcgttcc aggaaatcgt caagccgaac ataagaatgt agtgaaggca gcacaagcag
     2521 ctggggtatc ttatatcgct tatacaagtt ttgcagatgc cgatcactcg atcagtgtat
     2581 tagcgccaga tcaccaattc actgaaaaaa tgattaagga ttcaggtatt tcccatacat
     2641 ttttacggaa taattggtat gtagagaatg aattacctat cattgggcag gctttaaaaa
     2701 ctggtcaatt cgtttatgct gcaggaaatg gaaaaacagg ttgggcattg aaacgtgaat
     2761 atgcagaagc tgcagcaaaa gtactagtag aaagtgactc tccagaaatc ttagaattat
     2821 ccggcaatct aactgactat gaagaattag ccaaggcatt agagcgtgcg acaggtaaag
     2881 agttagaaat tattgaagca agtgatgcag cgtttgtcga aaatctaaaa gaagccggat
     2941 tccctcaaga agcagctgac atgtttttag cgatccaaca tgatattaaa aaggatcagt
     3001 tagctatttc ctctgatgat ttagaaaaag tattgggaaa accattaact tctcttgaag
     3061 cagcattaaa agaaatattg gatcaggaat aatgtataaa gaggttgtga aaaagccgat
     3121 tagttttgag tatgaagaaa aattttgtga aaaagctgtt ctgcatcaag taataagaag
     3181 agtcaaataa aaaatagctc ttcatatgtt tcgtttagct ggacttatta ccgcggagtt
     3241 ggcaggagaa cgccgttttt attcggaaaa aggagctgag acaagatttt tgtctcagct
     3301 cctgtttgtt tatcgataaa aaagtctggg gaagacgatg gctcaggctt tgttggaaac
     3361 agagccgatt ttatcatttg cttttacatc acgtgcgggt tcgatttcta agctttggac
     3421 tttgtcgcca tttgtgaata caacgaccat atccgtgttt ttgcctgcag attgaatagc
     3481 ggcaagatca actaaagcaa tcaattgacc tcttgcgact ttttgacctt cttctacata
     3541 taaagtgaat ggttcgcctt ttaagtcaac agtgttgatg cccatatgaa gcaagacttc
     3601 gataccagca tctgtttgga ttccgacagc atgtttcgtt gggaaaatgt ttgtgatcgt
     3661 accagcgatt ggagagaaga tttctccatt ttctggtaat acagcatagc cgtcacccat
     3721 cattttttcc gcgaatacat catctgaaac ttcagtgatt ggaatcagtt tcccatttgc
     3781 gatagcgtaa atatcttgtt cgatcgtttc accggcaaat gtttctgttt taaccacggt
     3841 gtttgtttgt gcaggtcgtg ctgcagcacc accatgtagt tttgccatct catctgcaac
     3901 gaattgtact tctgtaccta cgatgacttg cgcatttttt ccgtcgatga tcttcatacc
     3961 aggtacgcca gtggctttga ttttattttg atcgatcagg cctgtatctt tgacttgcaa
     4021 acgaaggcgt gtagtacagt tatcgatcga agttacattg tcggctccgc ctaatgcagc
     4081 gtagattttg cttgcttgtg ccgcatattt attgtctgaa ttagtcgttg cagtatcgtc
     4141 atctgaaaga tctgcttcct cgcggccagg agtcatcaga tggaattttt tgatagcaaa
     4201 gttaaagcca aagtagtaaa tcactgctgt tactaatcct tggacgatca acatcaatgg
     4261 ctgattcgca atcggatttt tcaaagatag gaagtaatcg acaaatccgg cactaaaggc
     4321 aaatcctgct gtccaatgga agaatgcact cactgctaaa gaaatcccag tgaaaattgc
     4381 atgcaagaca tataatggcc aagcaacgaa cataaatgaa aattcaagtg gttctgtcac
     4441 accagtaaag aatgaagcaa aagctgctgc tagcatcaag gaagctgttt ttgctttgtt
     4501 ttctggacgt gcatttctgt aaatagcata tgctcctgct ggaagcccga acatcatgat
     4561 tgggaaaaat ccagcttgat acattcctgt tacgcctttt gttccttcag aagccaaaaa
     4621 tttaccaata tcattgattc ctgctacgtc aaaccagaac acggagttca ttgcttgatg
     4681 caatcctgta gggatcagta aacggttgaa gaagccataa agacctgcac caccccagcc
     4741 taaactcaaa atgaatttag caaaagaaac taaagcagag taaaccggag gccatacgag
     4801 cagtagcact gcactgatca gcaacatgac aaaggcagcc aagattggca ctagacgctt
     4861 gccgctaaag aaagataaag ccatcggtaa ttttgcttcg tggaaacgat tataaagagc
     4921 tgccgcaatc aatccggcaa taataccaat aaatacgtta ctgatctgag caaaagatgg
     4981 atcaactgat tctggtttga tattcaataa agtagctact gaatcagttt ttaaaacatt
     5041 tactggaact tcaaatgcga ccaaacctgc caaggcagca gcacctgatt tgtccactga
     5101 catccctagt gctaacccaa cggcaaacag caaaggtaat tgacctaaaa ctgcgttacc
     5161 gccagcttgt aaaaaatgag cgacaatgtc gttactataa gatgcccacc agtttccgat
     5221 ccctactagt aaagcagcag caggtaagac cgcaactgga agttgaaggg aacgtcccat
     5281 tcgttgtaga tatgctttca atttttttcc tcctcatgat tgttaacatt tgtttgtacg
     5341 attgcgcttc ttaaacgctc gatatggatg gccaagtaac ccagttcttc tttcgtgatc
     5401 gcgagctggt attctttggt cagcaacaca cggatttttt tagaaatctt atagctttct
     5461 gggtattttc gtttgaccat ttctacgatt tccgtgtcta aaacagcata ttgttgcgca
     5521 tgggttcgtt gtagaagcag tcgtaaatga ttcaccagtc ttgaataatt cagtgaaaat
     5581 ggttctgaat ggatatctgt ttccaattct gattccacta aatggatgat gtcagataca
     5641 atcgtcactt cacggatact gcgatggtta ttgttccgac cgcttcgtcc gctgtgaata
     5701 tggattgcga tatatcctgc ttcgtcataa gtatatggaa tcccaagctc ttttgtcaga
     5761 tactctactg cccattgagc aatactgaat tcatcactgt aaagcacttc gatttctctt
     5821 aaaagtttgt tgcggatgac gataccgtta ttaatgttct ccgcagaaaa agcaatgtgg
     5881 tcggtcaaag cgatcaatac atgttcattc aacttttcca tcaaaacagt ttccgcatga
     5941 tcaatgatcg tttctgctgc aaataaaaag cgttcatcaa tctgattaag taaattttgc
     6001 aacttgattt gaccttctgg ttccatgaca aatatccgtt cgacatcacg atcaaaaatc
     6061 aaatcatttc tttttctgtc aaatccaatc ccttttccaa tagctacttt ttcctgtcca
     6121 ccgtcatcta ccaatactgc attttgatta aagatctttt tgattttcat gcgcgcaccc
     6181 tttttgaaga tttggttttc acgtttgcta gaccagttga ttgcggccaa acaaaaatag
     6241 acacgaaaaa cctcctaggg aaaccatgtt cctcagagat ttttcatgcc tagtatgatc
     6301 tagtcacatg aaacgaatta ttgttaacgt aatcaatttt atatagacag ttatgttttg
     6361 tcaattcttt ttttataatt atagtaattt actggaatta tcagaaataa aaggaagaaa
     6421 taaattaaga aacttatgtt atttaatcgt tggatctttt gcttatctta ttcgttgtta
     6481 aaaagctgaa agcgaaggaa aaaaactcta cttaactgta tttctccgtt aggacatcta
     6541 aaaatttacg agcagcagga gaaagaggct ggtctttttt ccaaatgaga ttcagtgaag
     6601 cttgtaatgg cggatcgagt gggatgaata ttttttccgt cccataagta ttgatgatcc
     6661 catctagagc taatgcacca aaagtcccgt tatcgaccat caaagaagca ttgtacaata
     6721 agttatatgt tccgacgatc gataactgat ctatgctgaa acctagccat tcagctagtt
     6781 gactgtccac tgacgtttgc cgggaaatga tcaaaggaac agttttcagt tcctgcggat
     6841 ggatgacggt ttgctttgct aaaggatgat ttcgatgtat caaaagtccc caacgatctt
     6901 gggctggcag tcgaaatgac tggtattttt gtttgtttgt cggttcgatt acgagtccaa
     6961 aatcaagtaa tccgctatcc agtttgtcta tgatatcatc tgcattcccg ctatataaat
     7021 gaaactggat atcaggatgc gccttgatca acgaatgaag gctatcagaa atgaaagaga
     7081 ttgctttagt ttcgcctccg ccaatgtaga tttctccaga gatgattgcg tcatttgtta
     7141 aattgttcgc cgttttttct accaacgaca agatttcttt tccttttgaa taaagatatc
     7201 gaccagaatc cgtcaactgt atctctcggg agccccgagt gaaaagtgtc acaccaagtt
     7261 cttcttctaa atctttcagt tgttttgaga gagtaggttg cgtgatatgc agagattttg
     7321 ctgctcggct gatatttttt tctctagcta ctgctaaaaa atacgtcagt atacgaatat
     7381 ccattttttc cctcctgttt ttaaaaaagc ataccactaa gtatagttaa aaggaatagt
     7441 taagtatgtt ttataagtat tagacatgat aagccgtccg ttataaagta aggataaaag
     7501 gagggagcaa aatgacaagc ctcatcgaaa aacaaatcgt caataaagag attcgagtaa
     7561 cggatccgct ctttgaagtg atccatcaga tccaagctga aaacgaagag cctttagcag
     7621 cgctgaatac tgggtaccat gaaccagcag atatccgcaa acggctagaa aatatcatta
     7681 gtgacaaagt agatgatacg gtgactgttc tgctgccttt ttatacagat ttcggcaaac
     7741 atattagtat agggaaaaat gtctttatca atcgtcaagt gatgttcgtt gacttaggtg
     7801 gcatctgttt agaagattca gtattgattg gtcctcgtgt caacctcatc acggtgaacc
     7861 atctaaccga tccaaaagaa cgaagaggct tgttagtaaa gccaatccac atcaagaaaa
     7921 atgcttggat cggagcagga gcaactattc tgccaggcgt aacgattggc gagaatgcca
     7981 ttgttgcagc gaatgcaact gttacgaaag atgtcccaga caataccatc gttgcaggaa
     8041 tccctgccaa aatcgtcaaa cctgtcgaac ggattatttg aacagtaaga tgaaataaga
     8101 gatagtgagt ataagcaaga gatttttcaa aacaggagga gaatagaatg aatgatttat
     8161 cagaaaaagt gattgtcatc atgggcgctt caagcggaat cggtgaagca acaacgaagc
     8221 tattagcaga aaaaggggct aaactagtta tagctgcacg ccgagaagat cgcctaaaag
     8281 caataaaaga atctttacca gaagctgagc tatatattca aacagcagat gtaagagatt
     8341 ttgcacaagt ccaagcagtt atcgatttag cgatggaaaa gttcggtcga atcgatgtat
     8401 tgtacaataa tgcaggaatc atgccaaccg ctccattagt tgaaggtcac cgtgatgaat
     8461 ggcaaaatat gcttgatatc aacatcatgg gtgtgttaaa tggaatttcc gccgtacttc
     8521 caatcatgga aaaacaaaaa tctgggcata tcatttcaac tgattctgtg gcgggacatg
     8581 ttgtttatcc tgattccgcc gtttattgcg gaacaaaatt tgctgtccgt gcaatcatgg
     8641 aaggattacg tcaagagcaa cggcaaaata acatcaaatc tacgatcatc tctccaggag
     8701 ctgttcaaac tgaattatat caaaccatca gcgacaaaaa ggctgctctt gaacttcatg
     8761 aagcgcaaaa agaatgggga ttgacctccg aagatatagc ttctgctgta gcatttgcga
     8821 tagaaacgcc agaccggatg tctgtcagta acatgattat ccgtccgacg acacaggaag
     8881 tataatccat aacggataat atccaaagag aattacttgt taaatagtta ttcaaacgac
     8941 catcaattta aggcgtgaat ggagaagaat attcttcatt tacgtctttt tatgtagaaa
     9001 aagcaaattt atcaaaaaga aacaaatgtt atttaaatat ggacattgac ttgcttttac
     9061 ttttttagtt acactgttat ttaggaaaag tttcataatt caaaggagaa aaaagatgaa
     9121 agcaaagaat ttattattta gcgttattct tttaggaaat ctagcagttg ccaacacaac
     9181 agtgctagct gaagaatcga cagaaactgt cgcaactcag acatcgagtt cttcagaaac
     9241 aggaattgaa aacgatacag agagtacttt gcagtcagct actgtctcag aaaatacaat
     9301 ttcaaccgaa gaaccaacaa ctgaaacaac agaacaaaca caaaccgaag acagtacatc
     9361 agcagaagct cagaacaatc cagagaatgc agaagaaatc acgaaccaat tgacagatgg
     9421 gcaaacattg acacataccc atcaagcaga tttgtctgaa ttcgaaaact atactggcta
     9481 tgctttttat gctcgctcat ctgctaattc tcaacaagcg ttcattgata gtattgcctc
     9541 aactgcacaa agtttggcaa gtgccaatga cttatatgca tcagtcatga ttgctcaagc
     9601 catcgttgaa agtggctggg ggaacagtac tttggcttct gcccctaatt acaatctatt
     9661 tgggattaaa ggaagctata atggccaatc tgttacgatg cctacttcgg aatatgtgaa
     9721 tgggcaatgg attacggtga atgccgcttt tagaaaatat ccttcgtaca aagaatcatt
     9781 gcaagataac gtaacggtat tgaagacgac ttcttttcag ccaggcgttt actactattc
     9841 cggtgcatgg aaaagcaata cgaactctta caaagatgct acggcttggc taacaggtcg
     9901 ttatgccact gcgcctaatt atggttctac attgaacaat gtaatcgaaa cctataatct
     9961 gacacagtat gatacagcac caacttcaac ttctccgatg tatcgtctgt ataaccgtca
    10021 tacaggagaa catttatata cattgaatgc cggagaaaaa aattatctgc caactgtagg
    10081 ctgggaatat gaagggattg cttggcaagc accaaacagc ggacaacctg tctaccgttt
    10141 atacaatcct tacagtggag accatcatta cacaatggca caatctgaga tcaactcttt
    10201 aacaaaaatc ggttggcgtt atgaaggtct aagtttttat tctggtggtt ccaagccaat
    10261 ttatcgacta tttaatccaa atgaaaaaac tggtacccat cactatacct tgagtgctaa
    10321 ggaaagagat tggctaacac cgatgggctg gagatatgaa ggaataggat tttatggtta
    10381 ttaaaaagag gtagtgaaaa agctgccctg catcaagtag taagaacagc caaacaaaaa
    10441 cagattttca tgttttttgt ttggttgaac ttattaccgc agatgcaagt ttttgaacac
    10501 cgtttactca aaaaaggttg tgacaacatc ttgtcataac ttttttatta ctgtttaagc
    10561 atactgatcc aatttctaat attcgacggg attcttgcaa aaggcgattt cggaaatttt
    10621 tggcggtgta aaattttcta ctgcagaaga gctatcttct atagtttgat aagaatcaat
    10681 aatcttggtt ttagcgttgt gctttcttta tcattttttc cgttcattct acaaaaaatt
    10741 tcttttctct atacttttct attgagagcg ttttttttat tacacagctg attctttgac
    10801 ttttttcttt ttttattcta ttattgacaa gtcaatcgtt gacgagtcaa ttattagaaa
    10861 ggaggaaatt atgtctttag aagagattag tcacctttta tatcaaatca aattagccga
    10921 tcaaaaaatc gtccagatct ttgaagaaaa tatcggtata agtcttacta gatacgagat
    10981 catgatgaga ctgaaagaag aaaatccatt gctacaaagc agtttgcaag aaaagttgca
    11041 gattgatcaa ggagcagtta ctcgtcatct gaaggtattg gaagcaaatg gctatgtttc
    11101 gcgtcagaga aatcctcaga acaaccggga agtgtatgta gcgctaacag aaaaagccca
    11161 gaagaaaatc gagcattgtg aaatggacca taaagataca ggttccgtgc tatccccggc
    11221 tttcagcgat gtagaaatga aacaatttct aacactttta gataaattga atacccaact
    11281 ttgcaatata acaacagaaa cgaaagaggt taataataat ggaacaaaaa aatgattttt
    11341 caacaattat gactggtaga agatccgttc gcgtatatga tgaaacatac aaaatcccac
    11401 acgaagaaat gctggaaatg atcaaagaag cgacgactgc accttcttct gtcaatatgc
    11461 aaccatggcg ctttgtggtc gctgaaagcg aggaagcaaa agaaatcttg cgtcctttga
    11521 tccgtttcaa tacgagacaa aatgatactt cttctgcaat ggtcatgatt tttggcgata
    11581 tgcaatgtta cgaatatggc gaagaaatct ataaccaagc ttacgaatca gggaagatgc
    11641 caaaagaagt cagagatcaa caattagctg cgatcattcc ttattacaag agtttttcaa
    11701 gagaagaaat gaacgatgta gtcaaagtcg attctagctt agcggctatg caattcatgt
    11761 tagttgcgcg tgatcatggc tatgaaacaa atccaattgg tggagtcgaa gcagatcagt
    11821 tggcagaggc atttggtttg gataaagacc gttatgttcc agttattatt ttatcaatag
    11881 gaaaagcggt agaagaaggt tacgagtctg ttcgtttagc accagaaaag atcactacgt
    11941 tcgaataaaa ggttttcagt tactattcag tagctattgc tttttttgaa tcgtgtgtgg
    12001 taacctaata aaaaaggagg tcggaaaaat gactgcatcg atgaaattac gtttcgaact
    12061 tttgaataac aactaaataa attcaaaaga catgtataaa caacaatttc atggatggag
    12121 tatgtccatt ttttcgaacg acagtagaat aggtgaaagc tatgacagat ttggtgattt
    12181 agtcatagtt atttcgttca gagtaggaaa agatgtgatg cagttttttt gcaaacagat
    12241 ttttctgctc ttttttagta tcgataattg ttgttttaca tgaaatagga gtgtaaaaca
    12301 tatggaaaat ttagcagtaa atataacaaa tctacaagtc agctttggca accagctaga
    12361 actatctatt gattctcttc gtgtctatca gcaagatcgt atagggatca taggagaaaa
    12421 tggtgtcggt aaatcgactt tgctcaaact aatagccggt gaactttttc ccgatcatgg
    12481 gaaaatccaa acagagatca ccttcaacta cctgcctcaa ttaacctatc tcgctgaagc
    12541 aaaggaccta aatttggaat tagccagtca tttccagtta agactggaag aaacttcaga
    12601 gcggaaatgg agcggagggg aagaacgaaa gatcgagttg atacgtcttc tttcttctta
    12661 tgaacaaggg atgcttctag acgagccaac aacccatcta gatagaaaaa gtattgatcg
    12721 actgattgaa gagcttcgtt attattatgg tacgctggtt tttgttagtc atgatcgcta
    12781 ttttctagat gagctagcat cgaaaatctg ggaagtaaaa gacggagaaa tccgagagtt
    12841 ttcggggaat tatagtgcct atctcactca aaaggaattg gagaaaaaga ctcagctacg
    12901 agaagcagag tcgatcatga aagagaaaaa acgattggaa aaatcgatcc aagaaaagaa
    12961 aaaacaagcg gaaaagttgg aaaaagtgtc cagtaaaaag aaaaagcaac aaatcagacc
    13021 ggatcggttg tcttcctcta aacaaaaaga cagtgtacaa aaagccatcc aaaagaatgc
    13081 gaaaacatta gagagaagac tccaaaaaat aggagaaaca accaagccgc agcagatgaa
    13141 acaaatccgt tttccagtac caaaatctct tgaactccac agccgctatc ccatcatggg
    13201 acaaaatgtc caattggaaa gaagtgggag aacattactg gtaaatggtg attttcagtt
    13261 ttctttaggt aaaaaaatcg cgattgtcgg tgaaaatggt tcaggtaaga caaccttatt
    13321 agaacatatc cgcaaacaag gagaaggaat ccttctctct ccgaaagtaa gctttcaagt
    13381 atatcagcaa aagggttatc aaatgacatc tgaagaatcc atcattcgtt ttgtcatgag
    13441 acaaacagag ttttcagaat cgcttgtccg tagtttgctg aatcacttag ggtttgctca
    13501 ggaaactttg acgaaaccgt tatgtacatt aagtggggga gaagcgaccc gtctgacgat
    13561 tgctttgctt tttactaagc caagtaatgt gttgctgtta gatgaaccga ctaattttat
    13621 tgatatggca acgatcgaag ctttagagaa gctaatgcaa atatatccgg gaacgatttt
    13681 gtttacttct catgattctt actttgtcga gcgtacggct gatgaagttt atgaaataaa
    13741 agggcagaaa ataaaaaaag tacttacgag aaatttttaa atgtgctacg atgattttcg
    13801 tgacaaattt aaaggagtgg tacagattgc ctacaaataa aaaagaagga ataattttta
    13861 caacaatgat gtgttttttg atggtcttgg ggatgagcgc gtataattta tggctgcatc
    13921 aagatcttca tttatcaagt ttgttgattg gtctagtgcc tggattcgtg gtggctttca
    13981 ttttggatgt cttcatcatc ggtgtgctgg cgaaaaaaat tgcctttaaa ctaccagtaa
    14041 atccagaaag caaggtccaa atgatcttaa ctatttcatg cttgatggtt ttaggaatgg
    14101 tgacatgtat gtctttattc ggtgtgatca tggaacaagg aatacctgat caattattct
    14161 ctgcttatat gaatgcttgg aaaatgaatg tgattgccgc tttaccgttg cagttgatca
    14221 tcgttggccc gctttcaaga agtgtgctaa aagcaattca aagtaatgaa tgatggaaca
    14281 ctgttatttg aaaaaaggcc ttggcatgct ttttgtcata gtctcttgaa tgcagttcta
    14341 gaaaaatagt gatccgaacg ataggttttg gcctgtcgtt cggatcgctt tttcttttcc
    14401 aactgtattg ctcttttatt gaagataatt tgctcttcaa tagatatttc tagaaaggtt
    14461 ctgtttatcc cttactcaaa aaactgctgc atttgttctc ttacttgctg acaccattgg
    14521 agttcctgct gacaaagcat aattctgcgc tggatcacaa cataacgtcc aaattcttgt
    14581 ttcttgtcta gcgagaggtc attcaatttt ttttgatttt tttgcatgat tttgttcagc
    14641 tgttgttctc tagtgaatag cagtccagta gctgttgttt tatcgagcgt cgaaaaagca
    14701 tagatttttg ctagaaattc atctttttgc gtaggagcgg gagtttcctc tttgatccat
    14761 tcctctacaa gagcacgtcc ttcatcagtc aagtggtaga tctttttttg agagtgctcg
    14821 tctttgtcca aaacggtcac ataaccctgc tggtctaact tggcaagcgt tgtatagatc
    14881 tgactgtgat gggcttccca aaaaattgtg atcagctgct tcaattcata cccagaacgt
    14941 ggtttggata aaagcacgca taataatata taggctaaag tattcataaa aatcctacct
    15001 ctcgtcttat tttctttatt atatcacagt aaagtttcca cgttttacat gtgcatattt
    15061 acatgtaaaa taaatcatgt atgatgctaa tagagagaaa gggatggaaa tattggagaa
    15121 tcaaatagat atacaagaac aaacaaatga agaaacaaag gggaaaagtt cagcgctagt
    15181 tgtagcagct acctgtatga tcgcttttat gggagtgggt ctagtagacc caatcttaaa
    15241 aacgattgct gcacagttaa acgctacgcc agctgaaacg accctgttat ttactagtta
    15301 catgttagtc actggtgtgg tgatgctatt tacaggtttc ttatctagtc ggatcggttt
    15361 gaaaaaaaca ttgatgacag gattgtttat catcgttgta ttcgctggtc ttggcggatt
    15421 atctgggaat atcggaatgc tgattgggtt acgtgccggc tggggtgtcg ggaatgcttt
    15481 atttgtctct actgctttag caacgatcgt cagtatttta gttggtaaca cagaaaaagc
    15541 aatcatgatg tatgaagcag cacttggctg cggaatggct gttggccctc tagtcggtgg
    15601 ggtcttgggc agtggttcat ggcgttatcc attttttggg gtagcatttt tgatgttttg
    15661 cggttttata gccctagcgg tacttttacc tgaaactgag aaaccaaaga gaaaagtagg
    15721 cctttgggaa ggtgtcaaag cgttaagcaa tcataaactt cgttcagttg gactaatcgc
    15781 attgttatat aatttcggat tctttacttt acttgcgtat gcgccatttt tattgattgg
    15841 ctattctgaa ttagaagtcg gttttgtatt ttttggctgg ggtgtgcttc tggctattgc
    15901 ttcgattttc gttgcaccgg ctcttgagcg gaagtttacg acttatcgga caattttagc
    15961 agcatttgtc ttattcatta tttgtttgct gttattagga gctggaactt ctatgccagt
    16021 ccttgtaccg atcagcattg tgttagcagg atttttccaa ggaattatca atacactgtt
    16081 gacgacgatt gctatggaaa taccaggtct gcaacgaaac gttgcttcgt ccagctatag
    16141 ttttgtccgc tttttcggtg gagctttagc accatttatt gcaggtgtga ttggtgaaca
    16201 tattggtgaa agctatgcgt tttattttgc aggtgccatt gttcttatca gtgtatgttg
    16261 tatcatttat caccgtaatt attttgctac aaatgaagaa gaataagaaa tcagaggttg
    16321 tgaaaaagct gtcctgcatc aagtcataag aa
//
LOCUS       DAYYGO010000319         8061 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.guided.118, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000319 DAYYGO010000000
VERSION     DAYYGO010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.guided.118"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(134..577)
                     /locus_tag="ACHA5T_003032"
     CDS             complement(134..577)
                     /locus_tag="ACHA5T_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFM8888503.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="ACHA5T_003033"
     CDS             complement(795..2135)
                     /locus_tag="ACHA5T_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8888504.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="ACHA5T_003034"
     CDS             2404..3300
                     /locus_tag="ACHA5T_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFM8888505.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="ACHA5T_003035"
     CDS             complement(3450..4391)
                     /locus_tag="ACHA5T_003035"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFM8888506.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="ACHA5T_003036"
     CDS             4517..5638
                     /locus_tag="ACHA5T_003036"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFM8888507.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /gene="eat(A)"
                     /locus_tag="ACHA5T_003037"
     CDS             5896..7398
                     /gene="eat(A)"
                     /locus_tag="ACHA5T_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFM8888508.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7465..7992
                     /locus_tag="ACHA5T_003038"
     CDS             7465..7992
                     /locus_tag="ACHA5T_003038"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8888509.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
BASE COUNT         2672 a         1504 c         1597 g         2288 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataaaaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag g
//
LOCUS       DAYYGO010000320         4777 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.guided.160, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000320 DAYYGO010000000
VERSION     DAYYGO010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.guided.160"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            149..955
                     /gene="vanY"
                     /locus_tag="ACHA5T_003039"
     CDS             149..955
                     /gene="vanY"
                     /locus_tag="ACHA5T_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-B/VanY-G family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HFM8888510.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGRR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            973..1800
                     /gene="vanW-B"
                     /locus_tag="ACHA5T_003040"
     CDS             973..1800
                     /gene="vanW-B"
                     /locus_tag="ACHA5T_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HFM8888511.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGEPFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            1797..2768
                     /locus_tag="ACHA5T_003041"
     CDS             1797..2768
                     /locus_tag="ACHA5T_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HFM8888512.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            2761..3789
                     /gene="vanB"
                     /locus_tag="ACHA5T_003042"
     CDS             2761..3789
                     /gene="vanB"
                     /locus_tag="ACHA5T_003042"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HFM8888513.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGTFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            3795..4403
                     /locus_tag="ACHA5T_003043"
     CDS             3795..4403
                     /locus_tag="ACHA5T_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HFM8888514.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(4469..4642)
                     /locus_tag="ACHA5T_003044"
     CDS             complement(4469..4642)
                     /locus_tag="ACHA5T_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888515.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT         1344 a         1014 c         1269 g         1150 t
ORIGIN      
        1 tgaccgacag gtataaccct gccggtcttt ttttgctgct acaggaaaac tacagactgg
       61 ctacagggaa agtacagatg cgcttgccat aataacaatc gtaccagcca caaatcgtag
      121 ttttattgca aaggaggcat tcgatcaaat ggaaaaaagc aactatcatt ccaatgcgga
      181 tcatcacaaa cggcatatga aacaatccgt ggaaaagcgg gcttttctat gtgcgttcat
      241 tatttcgttc acagtctgca cgctgttttt ggggcggaga ctggcttccg tattggaggc
      301 aacacagata ccgcccatcc ctgcaactca tacaggcagc agcactgacg tagtggagaa
      361 tttggaggaa aacgctcttg ccaccgccaa agaacaggga gatgaacagg aatggagcct
      421 gattttagtg aacaggcaga accccatccc cgcacagtac gatgtggagc ttgagcaact
      481 atcaaatggt gagcggatag atattcggat ttctccctat cttcaagatt tgtttgatgc
      541 cgcaagaact gatggagttt acccgattgt cgcatccgga taccgaacaa cagaaaaaca
      601 gcaagaaatt atggatgaaa aaattgccga atataaggcg aaaggctaca cctctgcaca
      661 ggctaaagcg gaagcagaaa cttgggtggc cgtgccggga acgagcgagc atcagcttgg
      721 tcttgctgtg gatatcaatg cggacggaat tcattcaaca ggcaacgagg tttatagatg
      781 gctggatgaa aacagctatc gttttggttt tattcgtcgc tacccaccag acaagacaga
      841 gataaccggt gtgagcaacg agccgtggca ttaccgatat gttggcatcg aagctgccac
      901 agagatgtac aaccaagggg tttgccttga ggaatattta aaaccagaaa aatgagaaaa
      961 ggatgttatg ttatggacag aaaaagattg acacagcgtt tcccgttcct gcttccaatg
     1021 agacgggcgc agagaaaaat gtgcttttat gcgggaatga gatttgacgg ctgtcgctat
     1081 gcgcagacga taggggaaaa atcgctttcc catttgctct ttgaaacgga ttgcgcatta
     1141 tacaaccaca ataccggatt tgacatgata taccaggaaa acaaggtgtt caacttaaag
     1201 ctggcagcaa agaccttaaa cggcttattg ataagaccgg gggaaccctt ttctttctgg
     1261 tggctggtac gccatgcgga caaagatacc ccctataaag acggccttac ggtgaccaat
     1321 ggtaaactca ccaccatgtc gggcggcggt atgtgccaga tgagcaattt actattttgg
     1381 atgttcctgc atacgccatt gacaattatc cagcgcagag gtcacgaagt aaaggagttt
     1441 ccagagccaa acagtgacga gattaaaggg gtggatgcaa ccatttcaga gggctggatt
     1501 gatttaaaag tgcgaaacga taccgactgc acctaccaaa tatgggtgac cttagatgat
     1561 gagaaaatca tcggtcaggt gtccgccgac aaagagcccc aagcattata caaaattaca
     1621 aatggcagta tccagtatgt ccgtgaaagt ggcgggattt atgaatatgc ccaggttaaa
     1681 cggatgcaag ttgccttagg taccggggaa ataatagatt gcaagctgct ttatacaaac
     1741 aaatgcaaaa tctgttatcc cctcccggaa agtgtggata ttcaggagga gaaccaatga
     1801 gaaaaagtgt gggcattact gtttttggat gcgaacagga tgaggcaaat gctttccgcg
     1861 ctttatcgcc ggattttcat attatcccta cgctgattag cgatgcgata tcggcagaca
     1921 acgcaaaatt ggccgctggc aatcaatgcg ttagcgtagg ccataagtcc gaggtttccg
     1981 aggcgacaat tcttgcgctg agaaaggtcg gggtaaaata catttctacc cgcagcatcg
     2041 gctgcgatca cattgatacg actgccgccg agagaatggg aatctcggtt ggcacggttg
     2101 cgtattcgcc ggacagcgtt gcggattatg ctttgatgct gatgctgatg gccatacggg
     2161 gtgcaaaacc caccatgcac gccgtggcgc aacaagattt cagattggat cgtatccggg
     2221 ggaaagaact gggggatatg actgtgggag ttattggaac cggccatatc gggcaagcgg
     2281 tcgtcaaaag gctgcgggga tttggatgcc atgtgctggc ctatgataac agccgaaaaa
     2341 tggatgcaga ttatgtccag cttgatgagc ttctaaaaaa cagcgatatt gttacgctcc
     2401 atgtgccgct ttgtgcggat acccgccatc tgatcggtca gaagcaaatt ggagagatga
     2461 agcaaggcgc atttttaatc aacaccgggc gcggagcgct tgtcgatacc ggggcgctgg
     2521 tggaggcgtt ggaaagcgga aagctgggtg gtgcggcact ggatgtgttg gagggcgagg
     2581 atcagtttgt ttataccgat tgctcgcaga aagtgcttga ccatcccttt ttgtcgcagc
     2641 ttttaaggat gccgaatgtg atcatcacac cccatacggc gtactacact gagcgtgtgc
     2701 tgcaggatac tacagaaaaa acaatcagga attgtctcaa ttttgaaagg agtttacagc
     2761 atgaatagaa taaaagtcgc aatcatcttc ggcggttgct cggaggaaca tgatgtgtcg
     2821 gtaaaatccg caatagaaat tgctgcgaac attgatacgg aaaaattcga tccgcactac
     2881 atcggaatta caaaaaacgg tgtatggaag ctatgcaaga agccatgtac ggaatgggaa
     2941 gccgacagtc tccccgccat actctccccg gataggaaaa cgcatgggct gcttgtcatg
     3001 aaagaaagcg aatacgaaac acggcgtatt gatgtggctt tcccggtttt gcatggcaaa
     3061 tgcggggagg atggtgcgat acaggggctg tttgtattgt ctggtatccc ctatgtgggc
     3121 tgtgatattc aaagctccgc agcttgcatg gacaaatcac tggcctacat tcttacaaaa
     3181 aatgcgggca tcgccgttcc cgaatttcaa atgattgata aaggtgacaa gccggaggcg
     3241 ggtgcgctta cctaccctgt ctttgtgaag ccggcacggt caggttcgtc ctttggcgta
     3301 accaaagtaa acggtacgga agaacttaac gctgcgatag aagcggcagg acaatatgat
     3361 ggaaaaatct taattgagca agcgatttcg ggctgtgagg tcgggtgtgc ggtcatgggg
     3421 aacgaggatg atttgattgt cggcgaagtg gatcaaatcc ggctgagcca cggtaccttc
     3481 cgcatccatc aggaaaacga gccggaaaaa ggctcagaaa atgcgatgat tacagttccc
     3541 gcagacattc cggtcgagga acgaaatcgg gtgcaggaaa cggcaaagaa agtatatcgg
     3601 gtgcttggat gcagagggct tgcccgtgtt gatctttttt tgcaggagga tggcggcatc
     3661 gttctaaatg aggtcaatac cctgcccggt tttacatcgt acagccgcta cccacgtatg
     3721 atggccgccg caggaatcac gcttcctgca ctgattgaca gcctgattac attggcgtta
     3781 aagaggtgat aagtatggaa aatggttttt tgtttttaga tgaaatgttg catggcgtcc
     3841 gttgggatgc caagtacgcc acatgggata acttcacagg aaaaccggtg gatgggtatg
     3901 aggtgaatcg catcatcggc acaaaggccg tggcgtttgc tctgcgcgag gcacaaatcc
     3961 atgcggctgc gcttggctat ggcttgcttt tatgggatgg atatcggcca agaactgcgg
     4021 tggactgctt cctgcgttgg gcagcgcaac cggaggacaa tctcacaaaa gaaaaatttt
     4081 accccaatat agagcgagcc gagttgatta caaagggtta tgtggcctca caatccagcc
     4141 atagccgtgg aagcgcaatt gatcttacgc tctaccacct ggatacaggg gaacttgttt
     4201 caatgggaag taacttcgat tttatggacg aacggtcgca tcatacagca aaagggatag
     4261 gggatgcaga ggcacaaaat cgaagatgct tgcgtaaaat catggaaagc agcggatttc
     4321 agtcttatcg ctttgaatgg tggcactata agttgattga tgagccatac cccgatacct
     4381 attttaattt tgccgtttca taatgaaagt attggttttg tatcagattt tggatttgat
     4441 aaaatctatg ctggtcatgg acaatctttt actggaaagt ggttaaagca tcttttgtaa
     4501 tacattgcaa attatagtat tcaatctgcc cagcggcaga aaagaaaaaa accgctgttg
     4561 ggcagacctt tcccacgccc attttgtatt ttgctggcgg cggttttgag aaatcaaggc
     4621 cgccgttctt ttctggcagc aaaaggtgtg gcggcccata ggaggatgcc gctatgctgt
     4681 tttgctattg ccatgctcta atccacttcc accactcaaa aaaagccttg cccctccatg
     4741 aactgaaccc caaaagttga actatttaat ggactgt
//
LOCUS       DAYYGO010000321         4452 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.guided.165, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000321 DAYYGO010000000
VERSION     DAYYGO010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.guided.165"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..237
                     /locus_tag="ACHA5T_003045"
     CDS             <1..237
                     /locus_tag="ACHA5T_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFM8888516.1"
                     /translation="ACNNAYCINEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFE
                     CYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI"
     gene            218..952
                     /locus_tag="ACHA5T_003046"
     CDS             218..952
                     /locus_tag="ACHA5T_003046"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8888517.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            985..1893
                     /locus_tag="ACHA5T_003047"
     CDS             985..1893
                     /locus_tag="ACHA5T_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFM8888518.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            1890..2432
                     /gene="sat4"
                     /locus_tag="ACHA5T_003048"
     CDS             1890..2432
                     /gene="sat4"
                     /locus_tag="ACHA5T_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HFM8888519.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            2525..3319
                     /locus_tag="ACHA5T_003049"
                     /note="aph(3')-IIIa"
     CDS             2525..3319
                     /locus_tag="ACHA5T_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFM8888520.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            3595..3726
                     /locus_tag="ACHA5T_003050"
                     /pseudo
     CDS             3595..3726
                     /locus_tag="ACHA5T_003050"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
     gene            3802..4295
                     /locus_tag="ACHA5T_003051"
                     /pseudo
     CDS             3802..4295
                     /locus_tag="ACHA5T_003051"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT         1538 a          674 c         1002 g         1238 t
ORIGIN      
        1 gcatgtaata atgcttattg cattaacgaa aagaaagcta taaaactgct tgaaactttt
       61 gaatataaac ctgaaaaata tgccgaaaga gttaatcata tttttgaagt actcggtctt
      121 tctctttttg aatgctacga catgaccgag aaactttata aagaagtgaa tgaaattgta
      181 tcggagataa ataacttttt aaacgaggag agttcagatg aaagaaaaca aatatgatga
      241 taatatattt tttcaaaaat acagtcaaat gagtcgctcg cagaaaggac tggctggtgc
      301 gggagaatgg gagactttga aaaagatgct acctgatttt aagggtaagc gtgtgcttga
      361 tttaggatgc ggctatggat ggcactgtat atatgcgatg gaaaacggtg cttcctctgt
      421 agtaggtgtt gatatttctc ataaaatgct cgaagtagca aaaggaaaaa cccattttcc
      481 acagattgaa tatgaatgct gtgccataga agatgtggat ttcccagagg agagctttga
      541 tgtaatacta agttcgcttg cgtttcatta tgtagcagac tatgagaatt taataaaaaa
      601 gatatatagg atgctgaagg ctggtggcaa tttagttttt acagttgaac atcctgtttt
      661 tactgctcat ggaacacaag actggtatta taacgaaaaa ggagaaatac tgcatttccc
      721 ggtggacaat tattattatg agggcaaacg gacagctatg tttttggaag aaaaggttac
      781 aaaatatcat agaacactga ccacatatct aaatacactg ctttcaaata gttttataat
      841 aaatcagatt gtggagccac agccgccaga gaacatgatg gatattccgg ggatggcgga
      901 tgaaatgcga cgcccaatga tgctgattgt atcggcaaaa aagaagatgt aataatatag
      961 aaaaaataaa cgaggagtat gtaaatgaga tcagaaaaag aaatgatgga tttagtactt
     1021 tctttagcag aacaggatga acgtattcga attgtgaccc ttgaggggtc acgcgcaaat
     1081 attaatatac ctaaagatga atttcaggat tatgatatta catattttgt aagtgatata
     1141 gaaccgttta tatctaatga tgactggctt aatcaatttg ggaatataat aatgatgcaa
     1201 aagccggagg atatggaatt attcccacct gaagaaaagg gattttccta tcttatgcta
     1261 tttgatgatt acaataaaat tgatcttacc ttattgccct tggaagagtt agataattac
     1321 ctaaagggcg ataaattaat aaaggttcta attgataaag attgtagaat taaaagggac
     1381 atagttccga ctgatataga ttatcatgta agaaagccaa gcgcaaggga gtatgatgat
     1441 tgctgcaatg aattttggaa tgtaacacct tatgttatta aaggattgtg ccgtaaggaa
     1501 attttatttg ctattgatca ttttaatcag attgttcgcc atgagctgct gagaatgata
     1561 tcatggaagg tcggcatcga aacaggcttt aaattaagtg taggcaagaa ctataagttt
     1621 attgaaaggt atatatccga ggatttgtgg gagaaacttt tgtccaccta ccggatggat
     1681 tcctatgaaa acatatggga agcattattt ctatgccatc aattgttcag ggcggtatcc
     1741 ggtgaggtgg cggaaaggct tcattatgcc tatccggagt atgataggaa tataacaaaa
     1801 tataccaggg acatgtataa aaaatacact ggtaaaaccg gctgcctgga tagcacatat
     1861 gccgctgata tagaagagag gcgggaacag tgattacaga aatgaaagca gggcacctga
     1921 aagatatcga taaacccagc gaaccatttg aggtgatagg taagattata ccgaggtatg
     1981 aaaacgagaa ttggaccttt acagaattac tctatgaagc gccatattta aaaagctacc
     2041 aagacgaaga ggatgaagag gatgaggagg cagattgcct tgaatatatt gacaatactg
     2101 ataagataat atatctttac taccaagacg ataaatgcgt cggaaaagtt aaactgcgaa
     2161 aaaattggaa ccggtacgct tatatagaag atatcgccgt atgtaaggat ttcagggggc
     2221 aaggcatagg cagcgcgctt atcaatatat ctatagaatg ggcaaagcat aaaaacttgc
     2281 atggactaat gcttgaaacc caggacaata accttatagc ttgtaaattc tatcataatt
     2341 gtggtttcaa aatcggctcc gtcgatacta tgttatacgc caactttgaa aacaactttg
     2401 aaaaagctgt tttctggtat ttaaggtttt agaatgcaag gaacagtgaa ttggagttcg
     2461 tcttgttata attagcttct tggggtatct ttaaatactg tagaaaagag gaaggaaata
     2521 ataaatggct aaaatgagaa tatcaccgga attgaaaaaa ctgatcgaaa aataccgctg
     2581 cgtaaaagat acggaaggaa tgtctcctgc taaggtatat aagctggtgg gagaaaatga
     2641 aaacctatat ttaaaaatga cggacagccg gtataaaggg accacctatg atgtggaacg
     2701 ggaaaaggac atgatgctat ggctggaagg aaagctgcct gttccaaagg tcctgcactt
     2761 tgaacggcat gatggctgga gcaatctgct catgagtgag gccgatggcg tcctttgctc
     2821 ggaagagtat gaagatgaac aaagccctga aaagattatc gagctgtatg cggagtgcat
     2881 caggctcttt cactccatcg acatatcgga ttgtccctat acgaatagct tagacagccg
     2941 cttagccgaa ttggattact tactgaataa cgatctggcc gatgtggatt gcgaaaactg
     3001 ggaagaagac actccattta aagatccgcg cgagctgtat gattttttaa agacggaaaa
     3061 gcccgaagag gaacttgtct tttcccacgg cgacctggga gacagcaaca tctttgtgaa
     3121 agatggcaaa gtaagtggct ttattgatct tgggagaagc ggcagggcgg acaagtggta
     3181 tgacattgcc ttctgcgtcc ggtcgatcag ggaggatatc ggggaagaac agtatgtcga
     3241 gctatttttt gacttactgg ggatcaagcc tgattgggag aaaataaaat attatatttt
     3301 actggatgaa ttgttttagt acctagattt agatgtctaa aaagctttaa ctacaagctt
     3361 tttagacatc taatcttttc tgaagtacat ccgcaactgt ccatactctg atgttttata
     3421 tcttttctaa aagttcgcta gatagagttc tatattagag atgtaaagag ttttggtgaa
     3481 gaggtttgtg agtatggctt ttataaccag ggtgcaatga gaagcactaa cagcagctag
     3541 cttaagaacg cataggatat actttttatg gaagtcacag aggaggaata aaatttgaac
     3601 aggattaaca gagtaacctc tattcttatt cagctgcaat caaaaaaaat aattcctgcc
     3661 aaagaaattg cacagcgatt taatataagc ttaaggacag tttacagaga tatccggaca
     3721 cttgaagaag ccggaatacc aattggatct gaggccggaa aaggatattt tcttgtagag
     3781 ggcttcctac ttccgccggt aatgtttaca gcggcggaag tgggtgcatt gattacagca
     3841 gggaaatttt taaattgcca tggagatgaa tcatttataa aggattttga ttcagctatg
     3901 tataaaatca aatctatttt gaagcatggt gaaaaaaact atgcacagga gttggagaac
     3961 agtattaacg tgtacagcac atctggacag aaaaatacat tggcggataa cgttattgca
     4021 gcaattcaga ctgcaatctg caataaaagg gtaatatcaa ttcaatatcc tgcatcagga
     4081 gggcaagaac cggaaagcag aatgatagag cctatatcac tggggtttta tgaacagaac
     4141 tggtacttaa tcggttttgc aggctaagaa atgaatatag gaattttcgt gtggatcgga
     4201 ttaaaagcat ttgcatagaa gaagagctct gtcaaagcca tgatggctct ttggaacaaa
     4261 tattaaaaca aatgctttca tataaaaaac tatatgccct acaatgccgt tgtaaaaatt
     4321 ggggaaaagg gatatattga ttttatatct ttctcaaaat aaatataaaa gccctttaaa
     4381 gagggctttt atatattaat cacaaatcac ttatcacaaa tcacaagtga ttaatcacaa
     4441 atcacttgtg at
//
LOCUS       DAYYGO010000322         3925 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.guided.174, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000322 DAYYGO010000000
VERSION     DAYYGO010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.guided.174"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..95
                     /locus_tag="ACHA5T_003052"
                     /pseudo
     CDS             6..95
                     /locus_tag="ACHA5T_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            409..456
                     /locus_tag="ACHA5T_003053"
                     /pseudo
     CDS             409..456
                     /locus_tag="ACHA5T_003053"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            472..2391
                     /gene="tet(M)"
                     /locus_tag="ACHA5T_003054"
     CDS             472..2391
                     /gene="tet(M)"
                     /locus_tag="ACHA5T_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFM8888521.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYTSGKLLEALELEQEESIRFHNCSLFPVYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIEIKEPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVA
                     QLPLGSGVQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2510..2677
                     /locus_tag="ACHA5T_003055"
     CDS             2510..2677
                     /locus_tag="ACHA5T_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFM8888522.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(2737..3090)
                     /locus_tag="ACHA5T_003056"
     CDS             complement(2737..3090)
                     /locus_tag="ACHA5T_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8888523.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            3595..3894
                     /locus_tag="ACHA5T_003057"
                     /pseudo
     CDS             3595..3894
                     /locus_tag="ACHA5T_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
BASE COUNT         1315 a          605 c          770 g         1235 t
ORIGIN      
        1 tctgatttgt ggtaaatcag gcaaaggcaa cgcaggtatc tcaatttgat ttggtacttg
       61 aaaagaacgg gagtaattgg aagattatag aataacaaat attggtacat tattacagct
      121 attttgtaat cacgtactct ctttgataaa aaattggaga ttcctttaca aatatgctct
      181 tacgtgctat tatttaagta tctatttaaa aggagttaat aaatatgcgg caaggtattc
      241 ttaaataaac tgtcaatttg atagtgggaa caaataattg gatgtccttt tttaggaggg
      301 cttagttttt tgtacccagt ttaagaatac ctttatcatg tgattctaaa gtatccggag
      361 aatatctgta tgctttgtat gcctatggtt atgcataaaa atcccagtga taagagtatt
      421 tatcactggg atttttatgc ccttttgggc ttttgaatgg aggaaaatca catgaaaatt
      481 attaatattg gagttttagc tcatgttgat gcgggaaaaa ctaccttaac agaaagctta
      541 ttatataaca gtggagcgat tacagaatta ggaagcgtgg acaaaggtac aacgaggacg
      601 gataatacgc ttttagaacg tcagagagga attacaattc agacaggaat aacctctttt
      661 cagtgggaaa atacgaaggt gaacatcata gacacgccag gacatatgga tttcttagca
      721 gaagtatatc gttcattatc agttttagat ggggcaattc tactgatttc tgcaaaagat
      781 ggcgtacaag cacaaactcg tatattattt catgcactta ggaaaatggg gattcccaca
      841 atctttttta tcaataagat tgaccaaaat ggaattgatt tatcaacggt ttatcaggat
      901 attaaagaga aactttctgc cgaaattgta atcaaacaga aggtagaact gtatcctaat
      961 atgtgtgtga cgaactttac cgaatctgaa caatgggata cggtaataga gggaaacgat
     1021 gaccttttag agaaatatac gtctgggaaa ttattggaag cattagaact cgaacaagag
     1081 gaaagcataa gatttcataa ttgttccctg ttccctgttt atcacggaag tgcaaaaaac
     1141 aatataggga ttgataacct tatagaagtg attacgaata aattttattc atcaacacat
     1201 cgaggtcagt ctgaactttg cggaaaagtt ttcaaaattg agtattcgga aaaaagacag
     1261 cgtcttgcat atatacgtct ttatagtggc gtactgcatt tgcgagattc ggttagaata
     1321 tcggaaaagg aaaaaataaa aattacagaa atgtatactt caataaatgg tgaattatgt
     1381 aaaatcgata aggcttattc cggggaaatt gttattttgc aaaatgagtt tttgaagtta
     1441 aatagtgttc ttggagatac aaaactattg ccacagagaa aaaagattga aaatccgcac
     1501 cctctactac aaacaactgt tgaaccgagt aaacctgaac agagagaaat gttgcttgat
     1561 gcccttttgg aaatctcaga tagtgatccg cttctacgat attacgtgga ttctacgaca
     1621 catgaaatta tactttcttt cttagggaaa gtacaaatgg aagtgattag tgcactgttg
     1681 caagaaaagt atcatgtgga gatagaaata aaagagccta cagtcattta tatggaaaga
     1741 ccgttaaaaa aagcagagta taccattcac atcgaagttc caccgaatcc tttctgggct
     1801 tccattggtc tatctgtagc acagcttcca ttagggagcg gagtacagta tgagagctcg
     1861 gtttctcttg gatacttaaa tcaatcgttt caaaatgcag ttatggaggg gatacgctat
     1921 ggctgtgaac aaggattgta tggttggaat gtgacggact gtaaaatctg ttttaagtat
     1981 ggcttatact atagccctgt tagtacccca gcagattttc ggatgcttgc tcctattgta
     2041 ttggaacaag tcttaaaaaa agctggaaca gaattgttag agccatatct tagttttaaa
     2101 atttatgcgc cacaggaata tctttcacga gcatacaacg atgctcctaa atattgtgcg
     2161 aacatcgtag acactcaatt gaaaaataat gaggtcattc ttagtggaga aatccctgct
     2221 cggtgtattc aagaatatcg tagtgattta actttcttta caaatggacg tagtgtttgt
     2281 ttaacagagt taaaagggta ccatgttact accggtgaac ctgtttgcca gccccgtcgt
     2341 ccaaatagtc ggatagataa agtacgatat atgttcaata aaataactta gtgtatttta
     2401 tgttgttata taaatatggt ttcttgttaa ataagatgaa atatttttta ataaagattt
     2461 gaattaaagt gtaaaggagg agatagttat tataaactac aagtggatat tgtgtcctgt
     2521 atgtggaaat aaaacacgat taaagataag ggaagatact gaattaaaaa aattccccct
     2581 ctattgtccg aaatgcagac aagaaaattt aattgaaata aagcagttca aagtaactgt
     2641 gattacagag ccagacgcaa agacgcagag ccgataaaat gagattaata caatctcatt
     2701 ttatcggctc tttccgttat gtatggattc ttttaattag tcttcgatgt ttcttgcttc
     2761 gttgataccg ctggctaaag attccattaa ggatagttct ttgtctgtaa agctatccat
     2821 gtatttctct atctgtaatc gtcgggtgct ttttaccaag ttattagcag gtaagaaaaa
     2881 ttcatcaacg gaaacatgaa gtaacgatac aaggtcataa agaacttgta tgctggggtg
     2941 ttgcccttta ttttcaatat tagttaagta ccgtgggtca atttcaatca atgctcccac
     3001 ttgttcacga gttaaacctc gtttcaatcg agcttcttta atggctaaac caaaggctct
     3061 aaaatcatat ttatcttctt ttttacgcat agtagaccac ctctatacat tttattgttc
     3121 ctactgaatt aaaaacaggt atagaaaaac gtgttatatg gtttataggt ttatatttaa
     3181 taaaaagcac tactaaacgc caataaaaaa aaccgttata tggtagtgct atttacgctg
     3241 ttaaaatatt gtatattact tccaaatggc ggtttgttgg aggtcaacgt cgccatgaag
     3301 tacatcatat acaataaatt tccttacatt gggttcttgt caaaaaaagt cgtctatctg
     3361 caatagataa gtacgtccac caatgtggtt ttataaatca tatagataga ataacagaag
     3421 catgtaaaca gagaaataaa tctgtttata tgcttttttg gctattcaga acttttttac
     3481 aaagtttatt tatcagtaat gcaacaaatc cccctttcac attgggacta agagtgaaag
     3541 gagataaacg agcaaggctc acttcctttc ctagacagaa agggggtgag aaacatgaaa
     3601 ccatcttctt ttcagaccac aatagaaaat cagtttgact atatctgtaa acgtgctatg
     3661 gaagacgagc gaaagaatta tatgctttat ctttcaagga ttgcaaagcg tgaggtgtcc
     3721 ttttcggatg ttggcgatta tcttgttagc cagtttgcga caacagataa ctattcaact
     3781 gactttcaga tttttacact caatgggtta tcagtaggcg ttgaaaatga tttgttgagt
     3841 gaagcattac gtgagttgcc agacaagaaa cgtgaaattc tactgctgtt ttacttatgg
     3901 gaatttcgct atttttagaa ttgcc
//
LOCUS       DAYYGO010000323         3510 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.guided.182, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000323 DAYYGO010000000
VERSION     DAYYGO010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.guided.182"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..653
                     /locus_tag="ACHA5T_003058"
     CDS             <1..653
                     /locus_tag="ACHA5T_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM8888524.1"
                     /translation="LCLIGYTGTRYANEIILWGGETDIDTINENLTQLDHTLTDKEQA
                     ITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQNLESQLVSKDAQLQ
                     SKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNTVNGLNQQIATLTQE
                     KTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTADKSQQIVEEHLRK"
     gene            980..1477
                     /gene="dfrG"
                     /locus_tag="ACHA5T_003059"
     CDS             980..1477
                     /gene="dfrG"
                     /locus_tag="ACHA5T_003059"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFM8888525.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(1549..3501)
                     /locus_tag="ACHA5T_003060"
     CDS             complement(1549..3501)
                     /locus_tag="ACHA5T_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFM8888526.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
BASE COUNT         1198 a          582 c          607 g         1123 t
ORIGIN      
        1 ttctatgtct aattggctac acaggcacgc ggtacgcaaa tgaaattatt ttgtggggtg
       61 gagagacaga tattgataca atcaatgaga atctgacaca gcttgatcat acattgacag
      121 ataaagagca agcgattaca gatcttaccg ctcgactagc acaaaaagaa cagacattgc
      181 aagattcaaa ggcagatgtc caagaatacc aaacaaaagt aaatgaatta gaaaaccaaa
      241 aaaatcaatc ggcagcagaa aaacaaaact tagaaagtca attagtcagc aaggatgcac
      301 aactacaaag caaacaaaat gaaatcaatg tcaaactaga tgagatcaac caaaaaaatg
      361 atgaaatcaa tcatctgaat gcagattgga cagcaagact acaagaagca caagcaaaac
      421 tttcagaatt acaaaatact gtaaatggtt tgaatcaaca aatcgcaaca ctgacacaag
      481 aaaaaacaaa tgtagaaact cagttaacag atacacaaaa gaaattagac gaagcaactg
      541 ctgaaaactc aagcttgaaa caatatattc aaaagttaga aaaagcaaaa gatgaagtgg
      601 aagatacagc tgataaatct cagcaaattg ttgaagaaca tttgcgtaaa taatctttct
      661 taacaagtaa acctaaatta gtaaagaagc aacagtaatc tatactaagt agttcagttt
      721 tggagtacaa aatgacaaaa tgtacttcaa aatggactac ttttttcatt ttaacttaca
      781 taccaaatta catacaaaat gatttataaa atgcaattga aaatgataat tgaatgaaaa
      841 tttgtgattt tgtggtatta tgataatata acttatagaa aaaattgctt attcaactaa
      901 cgggcaggat aatgagaaaa ggttttttga tagaaataag aaattagatg gtaaattagt
      961 taaaaaggct gtgttataaa tgaaagtttc tttgattgct gcgatggata agaatagagt
     1021 gattggcaaa gagaatgaca ttccttggag gattcccaag gactgggaat atgttaaaaa
     1081 tactacaaag ggacatccga taatattagg taggaagaac cttgaatcaa tcggaagagc
     1141 cttacctgac agaagaaata ttattctgac gagagataag gggtttacct ttaatggttg
     1201 tgaaattgtt cattcaatag aagatgtttt tgagttatgt aaaaacgaag aagaaatttt
     1261 tattttcgga ggagaacaga tttataattt gtttttccct tatgttgaga aaatgtacat
     1321 cacaaaaata catcatgaat tcgaaggaga tacttttttt ccagaagtga attatgagga
     1381 atggaatgag gtatttgccc aaaaagggat aaagaatgat aaaaatccgt ataactacta
     1441 ttttcatgta tatgaaagaa aaaacttatt gagttaacgg gtgcgttaat tgaagaagaa
     1501 cagtaaagat aaagcacaag agcttgggat tactccaagc tctttttgtc atttgaaata
     1561 aattatatct ttccaatcaa attgtttatc tgtaatccct aatctttgag caggtgtcaa
     1621 aaccttttta tccgctgatt taaaagggcg acaaaagttg tagtaagttc ttagaattgt
     1681 aatcgcaaat tgagcatact tcggattgaa gttagcgtaa atatagctct tcttatcacc
     1741 acgagctgtt gttaatggtc tttcaagaga tgatatataa cgtcttatct gttgaataaa
     1801 gctattggtc gaatggtcat taacatttaa tagcatcttt gcgatttcgt ttggttccag
     1861 agcagataaa tccgttctac aatcaactga ataaaatcct ttatccttag tggctaatgg
     1921 atgtttgata gggttatcgg catatttgcg atatctttct ccatctttac taatagcctc
     1981 ttcgtggaat tgatgacttg ttagtaattc agttagataa tgagatgcca aagtccgtag
     2041 aaactttgtt ttgaatcctc tgatatctcc ccaatcgagc agttctgctt ttgcttgtcc
     2101 gaattctttt aagcattgtt ttctgctttt cgtcttgtta acttggcaaa taaaatgatg
     2161 tgcatcgctc aaccttaatt ctttcgtgaa tactctataa aaggcgtttc taatagagaa
     2221 gtcatcatca ctaatcattc gccattcgtc ggcattaacc atttgtttaa ttaaccaata
     2281 gtgagcagtt gtagtataag gagcatcaac atttaaacca tcgacaaatc tactcctatt
     2341 agtaattttc cctaattcgt gaagataagc attcctattt tcgctatcgt tagcggatgg
     2401 ttcttgtgga taataagacc aatctaacct atcattttta cgactaaaag tattgagatg
     2461 gtcttctttg aattttcttg tatcttcatt taattcatcc attgaaatat tccaatcata
     2521 ggcaacatca gaacggaaca catatcttga taagacctca gcagatacaa ctatatacgt
     2581 ttgcatatta aggtcttcaa accccgtata tttctttgaa ccttgtcctt tcttacggac
     2641 gttatttagg tagtagtgca tcttgtccgt attaagccac atctcgttaa atttctttgt
     2701 ttgaaggggt tgtgtctcat atcgttctaa aaactccaaa caacgcctat acagccactc
     2761 cagcttatga tagtaggttc caactccaat tcctaaaata tcacaagtac gactgactga
     2821 aacccttcca acaaccatct tagcgaacat tggaagaatg gtgttctttt gttggtgata
     2881 agtagtggtt tcttctcttt ttggtagaac attagtgaac ttcttacacg ccttacattg
     2941 atagcgttga gatttgcccc tacttttccc ccttttgtaa aattgcttcg gctcattgaa
     3001 tggggttgat tcttcttccg agcagccttc tttatgaaat tgataatctg gctcaacatc
     3061 ttggatgcta ttgatttcta taaggcgttt aatttcttca acaacagacc aattagataa
     3121 aggggttact gtacagtttt gcgatacacc tctgccaata ggattgtcat tgcagtacat
     3181 acctttatga cctttatctt tggaactacc agtcatttta tatcgacttg gtttcccttt
     3241 tacatctttg aatttgtgct gttctttccc aaaattacaa caaaacggat tgatgcagta
     3301 attatattga atgggatatt cagaaccgtt ccatttaaaa gacgttggca agaacaacat
     3361 tttgttatac cttgttgcaa atcgttttgg agttaataaa tcatattgtt tagccctatc
     3421 ttttaattct tcgtttgaaa tcggaatttt aacatctaca atttcatctt ccttcgtggc
     3481 taatctttta agcttggtca ttgtctatca
//
LOCUS       DAYYGO010000324         2773 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.guided.198, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000324 DAYYGO010000000
VERSION     DAYYGO010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.guided.198"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            112..195
                     /locus_tag="ACHA5T_003061"
     CDS             112..195
                     /locus_tag="ACHA5T_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFM8888527.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            320..1057
                     /gene="erm(B)"
                     /locus_tag="ACHA5T_003062"
     CDS             320..1057
                     /gene="erm(B)"
                     /locus_tag="ACHA5T_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFM8888528.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1320..2183)
                     /locus_tag="ACHA5T_003063"
     CDS             complement(1320..2183)
                     /locus_tag="ACHA5T_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFM8888529.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2185..2457)
                     /locus_tag="ACHA5T_003064"
     CDS             complement(2185..2457)
                     /locus_tag="ACHA5T_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFM8888530.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2474..2683)
                     /locus_tag="ACHA5T_003065"
     CDS             complement(2474..2683)
                     /locus_tag="ACHA5T_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFM8888531.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
BASE COUNT          851 a          489 c          443 g          990 t
ORIGIN      
        1 ccttatttga tagtagattt aaaactttgc aacagaacca aaattttgta taataggaat
       61 tgaagttaaa ttagatgcta aaaatttgta attaagaagg agggattcgt catgttggta
      121 ttccaaatgc gtaatgtaga taaaacatct accgttttga aacagactaa aaacagtgat
      181 tacgcagata aataaatacg ttagattaat tcctaccagt gactaatctt atgacttttt
      241 aaacagataa ctaaaattac aaacaaatcg tttaacttct gtatttattt atagatgtaa
      301 tcacttcagg agtgattaca tgaacaaaaa tataaaatat tctcaaaact ttttaacgag
      361 tgaaaaagta ctcaaccaaa taataaaaca attgaattta aaagaaaccg ataccgttta
      421 cgaaattgga acaggtaaag ggcatttaac gacgaaactg gctaaaataa gtaaacaggt
      481 aacgtctatt gaattagaca gtcatctatt caacttatcg tcagaaaaat taaaactgaa
      541 cactcgtgtc actttaattc accaagatat tctacagttt caattcccta acaaacagag
      601 gtataaaatt gttgggaata ttccttacca tttaagcaca caaattatta aaaaagtggt
      661 ttttgaaagc catgcgtctg acatctatct gattgttgaa gaaggattct acaagcgtac
      721 cttggatatt caccgaacac tagggttgct cttgcacact caagtctcga ttcagcaatt
      781 gcttaagctg ccagcggaat gctttcatcc taaaccaaaa gtaaacagtg tcttaataaa
      841 acttacccgc cataccacag atgttccaga taaatattgg aagctatata cgtactttgt
      901 ttcaaaatgg gtcaatcgag aatatcgtca actgtttact aaaaatcagt ttcatcaagc
      961 aatgaaacac gccaaagtaa acaatttaag taccgttact tatgagcaag tattgtctat
     1021 ttttaatagt tatctattat ttaacgggag gaaataattc tatgagtcgc ttttgtaaat
     1081 ttggaaagtt acacgttact aaagggaatg tagataaatt attaggtata gggcacctct
     1141 aataactacc aattaattca cctcaaaatg aaattagaaa aaaacagcga aatttttaaa
     1201 tctatttctt atcgatacaa attcctcgta ggcgctcggg accccaacat gaggaaatgt
     1261 ttttataaaa aaagagtagt taccgttttt tggtaactac tcttttcctt ttttaaggtt
     1321 taaatacctg gaagtttagg tgttttgggt attggtggtg taggtagctg caagctttcc
     1381 attttttgtt gaattgcttt aaattcaggg gtttcttggt gttgattttg aaccattttt
     1441 tgctctattc tctccaaagt cggttgaatt tctttccctg atactttacg atttaattct
     1501 ctttctaagg tctctttcgg actaatggaa ggcgtttcta agcttgaata gagtttcact
     1561 ccttctctat tgtaaagcct tatatcgcta aataagcccg ttttatgaag ggtttctaaa
     1621 ttggtcggta agtttttgac aacaatatca tgcgcttgtt ttggtgttgc cctagctgtc
     1681 attggatcat ctgcatacat ggtttcatat cgttcaatcg ttcctaagta tgattcaatt
     1741 ttcggtactg ccatgacgta tatttttgtt tcataacctt tagattgaag cattgtggct
     1801 gtttgaatgg gaacgtctgt tgttcgtcct gttccttcaa tcactaaatt atagccttga
     1861 tcgcttaaac ggctaataag tgcttctgtc atgcgattcg aataaggggt aacgtgtttt
     1921 actacgtctt tttcatagag tttcactaat tcgtcaaaat ttgggtgctg ttgtttaaag
     1981 gtatcattat caatgacaat aacattccct tgtgtttctt caaaaattgc tgatcgcaaa
     2041 ctggttttcc ctgaccctgg ttgcccacca agtaaaaaag cggttggcga ttcaaccgct
     2101 agtctatttt tagttagtcg ttcaacattc ttttctaaac gatcttcaaa ttgcttttcg
     2161 gtgaagtcag taatattcgc catgttaagc cactttctct ttattcaaaa tttgttttga
     2221 atattgattc attgaccgcc aatactcatg aatagcttgt aattcttcaa atggttgttg
     2281 aaataaatca acttcttgtg ccacttctaa ttgatttaaa agattcactt ctagtaaacg
     2341 agtattttta gtatctagtt catgattcaa aacataattt aatacccgat tataaacact
     2401 tgccgataac tcattaatga tttcaatttc aaatgttttt tcatacgtaa ctgccatttc
     2461 attttcactc cttttaaagt ttgtcaggta aatatttccg tatatattct tctaaggctt
     2521 gatccataac ttcttttacg tttccgccat tctttgctgt ttcgattttt atgatatggt
     2581 gcaagtcggc acgaacacga accgtcttat cccccattat atcttttttt gcgctgattg
     2641 gcgtatcatt tcgttttgct ttctgtgcgc ctaaatttcc cacgatcgct cacttctttc
     2701 tatttcttct tactatttat tttatcatca cctatcacaa atcacaagtg atttgtgatt
     2761 aatcacttgt gat
//
LOCUS       DAYYGO010000325         2494 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.guided.205, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000325 DAYYGO010000000
VERSION     DAYYGO010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.guided.205"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..602
                     /locus_tag="ACHA5T_003066"
     CDS             <1..602
                     /locus_tag="ACHA5T_003066"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8888532.1"
                     /translation="LILDVSTKMFCEKGYDNTSLQDIINETKLSKGAIYHHFDSKEDI
                     LKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSVLLTVSPQLLNNP
                     RFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMVLSNVWLNPLVEM
                     TDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            709..1947
                     /gene="mef(H)"
                     /locus_tag="ACHA5T_003067"
                     /gene_synonym="mefH"
     CDS             709..1947
                     /gene="mef(H)"
                     /locus_tag="ACHA5T_003067"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
                     /protein_id="HFM8888533.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            2174..>2494
                     /locus_tag="ACHA5T_003068"
     CDS             2174..>2494
                     /locus_tag="ACHA5T_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016438386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HFM8888534.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDR"
BASE COUNT          814 a          345 c          439 g          896 t
ORIGIN      
        1 aactcatttt agatgtttct acaaaaatgt tttgtgaaaa aggatatgat aacacatcat
       61 tgcaagatat tatcaatgaa acaaaacttt ctaaaggtgc tatttatcat catttcgatt
      121 caaaggaaga tattttaaaa gcaatttttc aaagaattgg aaatgaaaat gcagtaatat
      181 ttgctaaaat tagagataac gatagtttaa acggtttaga aaagttaaga gaaatattta
      241 agacagcggt attcaattct aaccaaagcg ttttattgac agtttctcct caactattaa
      301 acaatcctcg ttttcttgct atgcaaatag aacaaatgta tgaaattgta gtacctgaat
      361 ttgttgaacc cgttttagaa caaggtatca aggataagtc tttggagatt gaaaattcac
      421 atgaaattgc agaagttatc atggtattaa gtaatgtatg gctcaaccca ttagttgaaa
      481 tgacggataa agagggtatg gaaaaacgtt gcaatacatt caatgccctt ttaaatggaa
      541 taggcataga cacattatta gacgaagaaa caatttctgc atttatacag ttttgtaaat
      601 aactgtattt gttatcccta tacgatagac tgctctataa aagagcagtt tatttaaacc
      661 attatataaa ccgacggtct gtttgattaa ataaaaagga gagattttat gcaagcagaa
      721 aaaaaacttt ttaatcgtga ttttatactt gttgttatag gacagataat ttctttattt
      781 ggaaacgcta tcctacgatt tgcattaccg tactatttgt taaaagaaac tggttcatca
      841 gctttgtttg gtattgctac agcctgctct tttttaccta tggttatttt gtcgctggta
      901 ggtggtatta ttgctgacag gacaaataaa agaaacatta tggtcatact agattttgta
      961 acagcaatca taattttagt attttatttg tcattgggaa aaattcctat catacctttg
     1021 tttattgttg ctttgatgtt actttatggt atttctggtg catatcaacc agcggtacaa
     1081 gcaagtattc ctctattggt atcatcagat aaacttaccg tcggtaatgc tataataaat
     1141 caagtaaata ctttagcaaa ctttatcgga ccagctataa gcggagtagt ttttcaattt
     1201 tatggtatca atcccatttt aattgttagt attatatgtt ttactttttc agccattatg
     1261 gaaattttta tccatattcc acaccaaaaa agacctcgtg aagtaggtgt aattgcagtt
     1321 gctaaaaacg atttaaaaga tagttatacc tttttgaaaa aggagaaacc catttttata
     1381 tccgtaatct ttttggtttg cattttcaat ttggttttaa gtgctgtttt aattgttggt
     1441 ctgcctatct taatttctga agttttaaaa atgccagata cactatatgg tttttcacaa
     1501 tcagcattat cattaggtgg tttatgtggt ggtattttaa cagctcttgt tgcttcaaag
     1561 ttaaaactaa aaaaccttta taccttgtta cttatttgtt ctgtttctgt tttacttatg
     1621 ggaatttcgc tatttttaga attgcctgca atagtttcct actgggtagt tacagtgatg
     1681 tgctttgtgg ctatgggtgt atctactatg tttgttgttc aaatttacac tcttgttcag
     1741 acacaaacac caccgcaatt agtaggaaag ataatggcta cgctggtagc agttgctatg
     1801 tgcggtcaac ctattggaca aatggtttac ggtattttat tcgatgtatt tatacaagaa
     1861 acatgggttg ttatgtttgt agcttctatt gcttcaatca tgatttcaat atattcaaaa
     1921 agaatttttc aaaagttaga gatataatac aaggggttaa atagtatgtt aggctaaaaa
     1981 caatatataa aagaaaattt gaaatctgct aaaaggatag tgttataaaa gaaagagtaa
     2041 tattgcagta tttaaggctc gcacaaatat atgttttgtg tgggcttttt tcttaccatt
     2101 tttctgtatt tctaaagggt ataaacggta ttccgtttat atatttcagc attagcagaa
     2161 agggggtgag attatgagga catcttcttt cgagcatatc gtcagattac agttcaattc
     2221 tttaatgctg attgtcatta agaacaaagt gaaaagtcga aacagacagc ttgcaagaca
     2281 ttctaaacgt gaagtattgt tttgtgaaat agccgaaacg aaacaagttg aacgtggaac
     2341 gattgatacc tgtttttatg attgtgtatc atttaaagtc ttgcacttta caatttatgt
     2401 atctgatgaa acacttggta cagttttaca tagactgtca gaaaaacagc gtagtgcgat
     2461 tttattacgt tattttcaag gaatgaacga caga
//
LOCUS       DAYYGO010000326         1663 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.guided.241, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000326 DAYYGO010000000
VERSION     DAYYGO010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.guided.241"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            77..426
                     /locus_tag="ACHA5T_003069"
                     /pseudo
     CDS             77..426
                     /locus_tag="ACHA5T_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(698..988)
                     /locus_tag="ACHA5T_003070"
     CDS             complement(698..988)
                     /locus_tag="ACHA5T_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888535.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1390..1626
                     /locus_tag="ACHA5T_003071"
     CDS             1390..1626
                     /locus_tag="ACHA5T_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8888536.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
BASE COUNT          555 a          286 c          283 g          539 t
ORIGIN      
        1 atatttccta atagagtata acatagcttt aagcattact taaagataac ttaaaagtta
       61 tcttttaaat acttaattgt taaatacctt atttgatgca aaagaaatct acgatagtca
      121 acaaaaaaat tttcattata tccagaaaaa tttttcttag ttaaggacct atggattgca
      181 gtaatgcaga attcgtcgaa taaactgcca aagacataca atcatattgc ttttaaaatt
      241 gacgaacaag atatagattc gtttgtctca aaaatccaaa tgttaggttt aactgtcgaa
      301 ccaggacgtt ctcgtgtgaa aggcgaggct tcttcaattt acttttacga ctatgataat
      361 cacctcttcg agttacatac cggtactctc caggaacgtt taaaatccta caattccact
      421 acctgagact agtaatgact agtctctttt tatggcctct gatcccccca ttgagcgaat
      481 actaataggg cttcgaattg atctgttcaa tctttaagca atatccctca aatgagtttg
      541 gaaagtttag tgattcatca caaatactta aatacatgta gttatatata gattgaggtt
      601 cttgtgagcg tatgctcgtg tgggttcaaa tcccatcgtc tgcatccatc tttccaaaac
      661 aagcggctag gacatttttg tctcagccgc ttgttttcta gtttattctt tcatcttgtc
      721 ggaccttttt tcttgggtat agacttttct catattctac cacaattttg ggtttcccta
      781 acacatgata ggatctcaca acaaattgct ttctattatt ataataacct gtaacgacta
      841 acttcatccc ttcgtctact tcagctagga agttcaaact atgggaagca atcaaacaat
      901 tgaccccatt taaactgaaa cggatcaacg gtctggcact aacttgcaaa gtacggatct
      961 tatctactgt tccatatttt ttctccataa ccttcacctc aataccatta tacgaacata
     1021 cattcccttt gtaaagaaga aactctgttt tatctttttg actttttgtc tattaccttt
     1081 cttaaaatca tattggcact tcctgcgaag atgaaggaca tgtactaaaa aaaagaggca
     1141 gcagtataga tttaagtagt tcagttttgg agtacaaaaa gtgaaaatgt aaacctaaat
     1201 gagtatcaaa ttgcaactga atttgatact cgttttttat tgttaaatca acgtttgttg
     1261 ttaatttaca ccacaaaatg taagttaaaa tgtatataaa aatgcgaacg tgaaataatt
     1321 attgtaggaa tgcaaattgg tgtgttaaaa tggttacaat ttactaagca aaaaggggtg
     1381 cattttccta tgcaatttct tgagccaaaa aataagaacg ctaagtctgt ggattgggaa
     1441 atatcagagc aagtaagggt gatagttaag caatacgctg aatatgcaga gcgtactgaa
     1501 agcgaagcag tggatgaatt cttattgaac attttagacg ataagaagtt tattgaatgg
     1561 attgccaaca agcgaagtaa taagcggatt gttgaaaaaa tgggtatcaa agatagagtg
     1621 ggttgataga caatgaccaa gcttaaaaga ttagccacga agg
//
LOCUS       DAYYGO010000327          877 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8341
            SAMEA115709692-rid22597393.guided.277, whole genome shotgun
            sequence.
ACCESSION   DAYYGO010000327 DAYYGO010000000
VERSION     DAYYGO010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709692
            Sequence Read Archive: ERR13199410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290508.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290508.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:30:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,012
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 51 of 88
            Pseudo Genes (internal stop)      :: 20 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709692-rid22597393.guided.277"
                     /isolate="VRE8341"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            264..812
                     /gene="aac(6')"
                     /locus_tag="ACHA5T_003072"
     CDS             264..812
                     /gene="aac(6')"
                     /locus_tag="ACHA5T_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFM8888537.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          312 a          128 c          183 g          254 t
ORIGIN      
        1 ttatttgtca attcataatt ggtttttttt aaagcaaaag ataatctata tcatgatgaa
       61 caaacagagt tttttcctcc aattgatggc tttcctgatc tttcctattt tgaataaagt
      121 gtttctgtat aattttttat tttataagta agataaataa tgaaagaata gagatactga
      181 ttggtagcta aagatttttg agcgagaaca tttcgttttt tcaaaaggac tgttagaata
      241 agcaagaaca agggggaaga cgtatgataa tcagtgaatt tgaccgtaat aatccagtat
      301 tgaaagatca gctttctgat ttactgagac tgacttggcc ggaagaatat ggagacagct
      361 cggcagaaga agtagaagaa atgatgaatc cagaacgaat cgcggtagca gcggtagacc
      421 aagatgagtt agtaggattt attggtgcaa tccctcaata cggtatcaca ggttgggaat
      481 tgcatccatt agttgtagaa agctcccgac gaaagaacca aataggtact cgattagtca
      541 attacttaga aaaagaagta gcttccagag gaggaatcac gatttattta ggtacggatg
      601 atttagacca tggaacaacg ttaagtcaaa cggacctgta tgagcataca tttgataaag
      661 tggcttccat ccagaacctt cgtgaacatc cgtatgaatt ctatgaaaaa ttaggttata
      721 aaatcgtagg tgtcttacca aatgcaaatg gctgggacaa accggatatt tggatggcaa
      781 aaacgattat tcctcgacca aattctcaat aataaaaaga ggttgtgaca gaagtaatca
      841 gcttcaagaa ataagaagga atttacgaaa attactt
//
